LOCUS       DAJSKJ010000001        79727 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000001 DAJSKJ010000000
VERSION     DAJSKJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.001"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..355
                     /locus_tag="ON345_000001"
     CDS             <1..355
                     /locus_tag="ON345_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR3070128.1"
                     /translation="CPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGT
                     AATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGIVNVDLIAYYVSTVEASEIT
                     PGEADAVVNVTLDYR"
     gene            435..1130
                     /locus_tag="ON345_000002"
     CDS             435..1130
                     /locus_tag="ON345_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR3070129.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1160..3676
                     /locus_tag="ON345_000003"
     CDS             1160..3676
                     /locus_tag="ON345_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR3070130.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPEHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVALEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADHQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3687..4777
                     /locus_tag="ON345_000004"
                     /pseudo
     CDS             3687..4777
                     /locus_tag="ON345_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4820..5680)
                     /gene="rsmI"
                     /locus_tag="ON345_000005"
     CDS             complement(4820..5680)
                     /gene="rsmI"
                     /locus_tag="ON345_000005"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR3070131.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            5744..7780
                     /gene="lpoA"
                     /locus_tag="ON345_000006"
     CDS             5744..7780
                     /gene="lpoA"
                     /locus_tag="ON345_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR3070132.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7738..8133
                     /locus_tag="ON345_000007"
     CDS             7738..8133
                     /locus_tag="ON345_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR3070133.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQALRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8153..8743
                     /gene="diaA"
                     /locus_tag="ON345_000008"
     CDS             8153..8743
                     /gene="diaA"
                     /locus_tag="ON345_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR3070134.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8753..9328
                     /gene="yraP"
                     /locus_tag="ON345_000009"
     CDS             8753..9328
                     /gene="yraP"
                     /locus_tag="ON345_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR3070135.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9442..10482)
                     /locus_tag="ON345_000010"
     CDS             complement(9442..10482)
                     /locus_tag="ON345_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3070136.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10555..11190)
                     /locus_tag="ON345_000011"
     CDS             complement(10555..11190)
                     /locus_tag="ON345_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR3070137.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11318..11835
                     /gene="yhbO"
                     /locus_tag="ON345_000012"
                     /pseudo
     CDS             11318..11835
                     /gene="yhbO"
                     /locus_tag="ON345_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
     gene            complement(11815..12258)
                     /locus_tag="ON345_000013"
     CDS             complement(11815..12258)
                     /locus_tag="ON345_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR3070138.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12309..12611
                     /gene="yhbQ"
                     /locus_tag="ON345_000014"
     CDS             12309..12611
                     /gene="yhbQ"
                     /locus_tag="ON345_000014"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR3070139.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12598..13101)
                     /locus_tag="ON345_000015"
     CDS             complement(12598..13101)
                     /locus_tag="ON345_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3070140.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13095..13619)
                     /locus_tag="ON345_000016"
     CDS             complement(13095..13619)
                     /locus_tag="ON345_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR3070141.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13828..14823
                     /locus_tag="ON345_000017"
     CDS             13828..14823
                     /locus_tag="ON345_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3070142.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14832..15710
                     /locus_tag="ON345_000018"
     CDS             14832..15710
                     /locus_tag="ON345_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3070143.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            15791..16798
                     /locus_tag="ON345_000019"
     CDS             15791..16798
                     /locus_tag="ON345_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR3070144.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVVAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16916..18160)
                     /gene="mtr"
                     /locus_tag="ON345_000020"
     CDS             complement(16916..18160)
                     /gene="mtr"
                     /locus_tag="ON345_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR3070145.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18314..20203)
                     /gene="deaD"
                     /locus_tag="ON345_000021"
     CDS             complement(18314..20203)
                     /gene="deaD"
                     /locus_tag="ON345_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR3070146.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20196..20276)
                     /gene="yrbN"
                     /locus_tag="ON345_000022"
     CDS             complement(20196..20276)
                     /gene="yrbN"
                     /locus_tag="ON345_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR3070147.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20383..21267)
                     /gene="nlpI"
                     /locus_tag="ON345_000023"
     CDS             complement(20383..21267)
                     /gene="nlpI"
                     /locus_tag="ON345_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR3070148.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21376..23511)
                     /gene="pnp"
                     /locus_tag="ON345_000024"
     CDS             complement(21376..23511)
                     /gene="pnp"
                     /locus_tag="ON345_000024"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR3070149.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTSVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23758..24027)
                     /gene="rpsO"
                     /locus_tag="ON345_000025"
     CDS             complement(23758..24027)
                     /gene="rpsO"
                     /locus_tag="ON345_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR3070150.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24176..25120)
                     /gene="truB"
                     /locus_tag="ON345_000026"
     CDS             complement(24176..25120)
                     /gene="truB"
                     /locus_tag="ON345_000026"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR3070151.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25120..25521)
                     /gene="rbfA"
                     /locus_tag="ON345_000027"
     CDS             complement(25120..25521)
                     /gene="rbfA"
                     /locus_tag="ON345_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR3070152.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25685..28357)
                     /gene="infB"
                     /locus_tag="ON345_000028"
     CDS             complement(25685..28357)
                     /gene="infB"
                     /locus_tag="ON345_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR3070153.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28382..29869)
                     /gene="nusA"
                     /locus_tag="ON345_000029"
     CDS             complement(28382..29869)
                     /gene="nusA"
                     /locus_tag="ON345_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR3070154.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29897..30349)
                     /gene="rimP"
                     /locus_tag="ON345_000030"
     CDS             complement(29897..30349)
                     /gene="rimP"
                     /locus_tag="ON345_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR3070155.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30556..30632)
                     /locus_tag="ON345_000031"
     tRNA            complement(30556..30632)
                     /locus_tag="ON345_000031"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30596..30598),aa:Met,seq:cat)
     gene            30980..32323
                     /gene="argG"
                     /locus_tag="ON345_000032"
     CDS             30980..32323
                     /gene="argG"
                     /locus_tag="ON345_000032"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR3070156.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPTEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(32331..33956)
                     /locus_tag="ON345_000033"
     CDS             complement(32331..33956)
                     /locus_tag="ON345_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR3070157.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34415..34501)
                     /locus_tag="ON345_000034"
     tRNA            complement(34415..34501)
                     /locus_tag="ON345_000034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34465..34467),aa:Leu,seq:gag)
     gene            complement(34516..34848)
                     /gene="secG"
                     /locus_tag="ON345_000035"
     CDS             complement(34516..34848)
                     /gene="secG"
                     /locus_tag="ON345_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR3070158.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35076..36413)
                     /gene="glmM"
                     /locus_tag="ON345_000036"
     CDS             complement(35076..36413)
                     /gene="glmM"
                     /locus_tag="ON345_000036"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR3070159.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36406..37254)
                     /gene="folP"
                     /locus_tag="ON345_000037"
     CDS             complement(36406..37254)
                     /gene="folP"
                     /locus_tag="ON345_000037"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR3070160.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37344..39278)
                     /gene="ftsH"
                     /locus_tag="ON345_000038"
     CDS             complement(37344..39278)
                     /gene="ftsH"
                     /locus_tag="ON345_000038"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR3070161.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39378..40007)
                     /gene="rlmE"
                     /locus_tag="ON345_000039"
     CDS             complement(39378..40007)
                     /gene="rlmE"
                     /locus_tag="ON345_000039"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR3070162.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40133..40426
                     /gene="yhbY"
                     /locus_tag="ON345_000040"
     CDS             40133..40426
                     /gene="yhbY"
                     /locus_tag="ON345_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR3070163.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40582..41058)
                     /gene="greA"
                     /locus_tag="ON345_000041"
     CDS             complement(40582..41058)
                     /gene="greA"
                     /locus_tag="ON345_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR3070164.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41306..42739
                     /gene="dacB"
                     /locus_tag="ON345_000042"
     CDS             41306..42739
                     /gene="dacB"
                     /locus_tag="ON345_000042"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR3070165.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42779..43951)
                     /gene="cgtA"
                     /locus_tag="ON345_000043"
     CDS             complement(42779..43951)
                     /gene="cgtA"
                     /locus_tag="ON345_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR3070166.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43967..44932)
                     /locus_tag="ON345_000044"
     CDS             complement(43967..44932)
                     /locus_tag="ON345_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR3070167.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45059..45316)
                     /gene="rpmA"
                     /locus_tag="ON345_000045"
     CDS             complement(45059..45316)
                     /gene="rpmA"
                     /locus_tag="ON345_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR3070168.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45337..45648)
                     /gene="rplU"
                     /locus_tag="ON345_000046"
     CDS             complement(45337..45648)
                     /gene="rplU"
                     /locus_tag="ON345_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR3070169.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45907..46878
                     /gene="ispB"
                     /locus_tag="ON345_000047"
     CDS             45907..46878
                     /gene="ispB"
                     /locus_tag="ON345_000047"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR3070170.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47106..47384
                     /gene="sfsB"
                     /locus_tag="ON345_000048"
     CDS             47106..47384
                     /gene="sfsB"
                     /locus_tag="ON345_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR3070171.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47432..48691)
                     /gene="murA"
                     /locus_tag="ON345_000049"
     CDS             complement(47432..48691)
                     /gene="murA"
                     /locus_tag="ON345_000049"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3070172.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48746..49000)
                     /gene="ibaG"
                     /locus_tag="ON345_000050"
     CDS             complement(48746..49000)
                     /gene="ibaG"
                     /locus_tag="ON345_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR3070173.1"
                     /translation="MENNEIQSLLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49160..49453)
                     /gene="mlaB"
                     /locus_tag="ON345_000051"
     CDS             complement(49160..49453)
                     /gene="mlaB"
                     /locus_tag="ON345_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR3070174.1"
                     /translation="MSESLSWMQTGDTLALFGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49453..50088)
                     /gene="mlaC"
                     /locus_tag="ON345_000052"
     CDS             complement(49453..50088)
                     /gene="mlaC"
                     /locus_tag="ON345_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR3070175.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50107..50658)
                     /gene="mlaD"
                     /locus_tag="ON345_000053"
     CDS             complement(50107..50658)
                     /gene="mlaD"
                     /locus_tag="ON345_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR3070176.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50663..51445)
                     /gene="mlaE"
                     /locus_tag="ON345_000054"
     CDS             complement(50663..51445)
                     /gene="mlaE"
                     /locus_tag="ON345_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR3070177.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51453..52262)
                     /gene="mlaF"
                     /locus_tag="ON345_000055"
     CDS             complement(51453..52262)
                     /gene="mlaF"
                     /locus_tag="ON345_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR3070178.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52472..53449
                     /locus_tag="ON345_000056"
     CDS             52472..53449
                     /locus_tag="ON345_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR3070179.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53463..54449
                     /gene="kdsD"
                     /locus_tag="ON345_000057"
     CDS             53463..54449
                     /gene="kdsD"
                     /locus_tag="ON345_000057"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR3070180.1"
                     /translation="MSHVELQPGFDFQQVGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54470..55036
                     /gene="kdsC"
                     /locus_tag="ON345_000058"
     CDS             54470..55036
                     /gene="kdsC"
                     /locus_tag="ON345_000058"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR3070181.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55033..55608
                     /gene="lptC"
                     /locus_tag="ON345_000059"
     CDS             55033..55608
                     /gene="lptC"
                     /locus_tag="ON345_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR3070182.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55577..56134
                     /gene="lptA"
                     /locus_tag="ON345_000060"
     CDS             55577..56134
                     /gene="lptA"
                     /locus_tag="ON345_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR3070183.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56141..56866
                     /gene="lptB"
                     /locus_tag="ON345_000061"
     CDS             56141..56866
                     /gene="lptB"
                     /locus_tag="ON345_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR3070184.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIKHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56914..58347
                     /gene="rpoN"
                     /locus_tag="ON345_000062"
     CDS             56914..58347
                     /gene="rpoN"
                     /locus_tag="ON345_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR3070185.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58370..58657
                     /gene="hpf"
                     /locus_tag="ON345_000063"
     CDS             58370..58657
                     /gene="hpf"
                     /locus_tag="ON345_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR3070186.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58775..59266
                     /gene="ptsN"
                     /locus_tag="ON345_000064"
     CDS             58775..59266
                     /gene="ptsN"
                     /locus_tag="ON345_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR3070187.1"
                     /translation="MTNDDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59312..60166
                     /gene="rapZ"
                     /locus_tag="ON345_000065"
     CDS             59312..60166
                     /gene="rapZ"
                     /locus_tag="ON345_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3070188.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60163..60435
                     /gene="npr"
                     /locus_tag="ON345_000066"
     CDS             60163..60435
                     /gene="npr"
                     /locus_tag="ON345_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR3070189.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60649..61281
                     /gene="yrbL"
                     /locus_tag="ON345_000067"
     CDS             60649..61281
                     /gene="yrbL"
                     /locus_tag="ON345_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR3070190.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61278..62006)
                     /gene="mtgA"
                     /locus_tag="ON345_000068"
     CDS             complement(61278..62006)
                     /gene="mtgA"
                     /locus_tag="ON345_000068"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR3070191.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFLEHWGFNVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62003..62656)
                     /gene="elbB"
                     /locus_tag="ON345_000069"
     CDS             complement(62003..62656)
                     /gene="elbB"
                     /locus_tag="ON345_000069"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR3070192.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62886..65222)
                     /gene="arcB"
                     /locus_tag="ON345_000070"
     CDS             complement(62886..65222)
                     /gene="arcB"
                     /locus_tag="ON345_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR3070193.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65318..66247)
                     /locus_tag="ON345_000071"
     CDS             complement(65318..66247)
                     /locus_tag="ON345_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR3070194.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            66922..71382
                     /gene="gltB"
                     /locus_tag="ON345_000072"
     CDS             66922..71382
                     /gene="gltB"
                     /locus_tag="ON345_000072"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR3070195.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71395..72813
                     /gene="gltD"
                     /locus_tag="ON345_000073"
     CDS             71395..72813
                     /gene="gltD"
                     /locus_tag="ON345_000073"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR3070196.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            72997..74109
                     /locus_tag="ON345_000074"
     CDS             72997..74109
                     /locus_tag="ON345_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR3070197.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSTRNLWQFKNFYICFQRIEI
                     VQTLSAQFPPTIRQTLSGESCHLAQLAKSFPLPWSTYVRLLSVKNADARSFYEKETLR
                     CGWSVRQLERQIATQFYERILLSHDKSAMLQQHAPTETHILPQQAIRDPFVLEFLELK
                     DEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLL
                     IVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYALAGLP
                     NTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74169..74633)
                     /locus_tag="ON345_000075"
     CDS             complement(74169..74633)
                     /locus_tag="ON345_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR3070198.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74630..75505)
                     /gene="nanK"
                     /locus_tag="ON345_000076"
     CDS             complement(74630..75505)
                     /gene="nanK"
                     /locus_tag="ON345_000076"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR3070199.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75502..76191)
                     /locus_tag="ON345_000077"
     CDS             complement(75502..76191)
                     /locus_tag="ON345_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3070200.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76239..77729)
                     /gene="nanT"
                     /locus_tag="ON345_000078"
     CDS             complement(76239..77729)
                     /gene="nanT"
                     /locus_tag="ON345_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR3070201.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77838..78731)
                     /gene="nanA"
                     /locus_tag="ON345_000079"
     CDS             complement(77838..78731)
                     /gene="nanA"
                     /locus_tag="ON345_000079"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR3070202.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78853..79644)
                     /gene="nanR"
                     /locus_tag="ON345_000080"
     CDS             complement(78853..79644)
                     /gene="nanR"
                     /locus_tag="ON345_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR3070203.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19623 a        21593 c        19785 g        18726 t
ORIGIN      
        1 ctgcccagtg actgggtggg agcacgataa cggcaacgtt gaagcgtctt tccgtttatg
       61 gctggaaacg cgggataatg gcaccgtccc taacttccct aatctggcaa aagtgggctc
      121 ttttgctggc acagcggcaa caggtgtggg tatccgtatt gacgatgcgg aaagcggaaa
      181 ccttatgcca ctgaatgcta tggggaatga taacacggtt tatcagatcc cagcggactc
      241 caatggtatt gtcaatgttg acctcatcgc ttactacgta tcaaccgttg aagcatcaga
      301 aatcacccca ggggaagcag acgctgtcgt taacgtaacg ctggattacc gttaatacgt
      361 tacggcgtta tctgaccttt cagataacgc ccttttcctt cctctttctc gttgtatcag
      421 gttgaaaaat aactatgtca aaacgaacat tcgcggtgat aataaccttg ttgtgtagct
      481 tctgtatcgg ccaggcgctt gcagggggta tcgttttaca gcgaacgcga gtgatctatg
      541 atgccagccg caaagaggct gcgttacctg tcgcaaacaa aggcgcagaa acgccttatt
      601 tactgcaatc atgggtagat aatatagatg gtacaagccg tgccccattt attataaccc
      661 caccgctatt tcgtcttgaa gctggcgatg actcatcact gcgaattatt aaaacagctg
      721 ataacctgcc tgaaaataaa gagtcgctgt tctacattaa tgttcgtgcc attccagcaa
      781 agaaaaaatc agatgatgtt aatgctaacg agttgacgct ggtatttaaa acacggatca
      841 aaatgtttta tcgccccgca cacctgaagg gacgggtaaa cgatgcgtgg acatcactgg
      901 aatttaaacg tagtgaccat tcactcaata tatataaccc aactgaatat tacgtcgtat
      961 ttgccggact ggcagtcgat aaaaccgatc tcacaagcaa aattgaatat atcgcgcccg
     1021 gagaacataa acagttacca cttcctgcat ctggcggaaa gaacgtgaaa tgggctgcaa
     1081 tcaatgatta tggcggcagt tccgggacag aaactcgtcc actgcaataa caaatataaa
     1141 aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac gcacaccgaa
     1201 acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca gcggcatgct
     1261 ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg aaactacaaa
     1321 aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac aactaccagg
     1381 aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa aaattacatt
     1441 taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac tacgtgagct
     1501 gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata gcgtgatcaa
     1561 ctcgcttgaa caaatcattc ccggtacagc tgctgaattt gatttcaatc atcagcgact
     1621 taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg tctccccttc
     1681 tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag gttctgataa
     1741 ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa atggtgctaa
     1801 ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc agacatcaag
     1861 ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt ctcagttgct
     1921 tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta ctggcgttca
     1981 actcgcttcc gacgataata tgctgccaaa cagccagcgc ggatttgccc caacggtacg
     2041 cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg tgatctatca
     2101 aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt ccaacagcgg
     2161 cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta tccagcctta
     2221 ttcttcatta cccatgatgc agcgacctga gcatctaaaa tatagcgcga ccgctggacg
     2281 ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag ccacggcaat
     2341 atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg aagattatta
     2401 tgcgttgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt cgatggatat
     2461 caacagagct gacacccaat tcgataacca gcactctttt catggctatc aatggcgtac
     2521 gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct actatcgcta
     2581 taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga actataacag
     2641 tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg gggtaagtct
     2701 gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca ggaatatctc
     2761 agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc aatacagtcg
     2821 ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc cgttagaacg
     2881 ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc gcccaaccca
     2941 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
     3001 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
     3061 ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat acaattacgg
     3121 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
     3181 cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa actatccggg
     3241 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt accaggaaaa
     3301 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gctcttgagc aaacaacaca
     3361 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
     3421 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
     3481 cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
     3541 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcatca
     3601 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
     3661 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
     3721 tgatatccac atcctgcgct tatgcctcct caggagggtg tggagccgac agcactagcg
     3781 gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca gataacgtga
     3841 caggacggga gtttacctct gcaacgctaa gtagcactaa ctggcaatac gcctgttcct
     3901 gctctgcggg taaggcagtt aaacttgtct atatggtcag ccccgtactt accaccactg
     3961 gacatcagac aggatattac aaactcaatg acagcctgga tattaaaacc acattacagg
     4021 caaacgacat tccaggactc acaaccgacc aggttgtctc tgttaacacc cgattcacac
     4081 agataaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     4141 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     4201 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     4261 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     4321 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaggt
     4381 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     4441 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     4501 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     4561 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     4621 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     4681 cgtcaatatg cgcgcctagc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     4741 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     4801 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     4861 tcttcacgcc atgaatttct gcgaccagcg ccgccgcttt tttcagcggc agttctgcct
     4921 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     4981 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     5041 gcagttcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     5101 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     5161 ggtgggtgga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     5221 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagagggta
     5281 aacccgcggc gcttaacgca gtgatagcag cacacggccc gggtagcggc accacacgaa
     5341 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     5401 cggcatcgga aaccagcgca atgttttgcc cctcttgcag cttcgccagc agcgtttcgg
     5461 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     5521 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     5581 cctctaacgc tcgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     5641 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     5701 tttaatattg agcattgcgt aaaaaaatat cactggatac attatggtac cctcaacatt
     5761 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     5821 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggtacgg cgcaggctga
     5881 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     5941 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     6001 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggt
     6061 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     6121 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     6181 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgctcgg
     6241 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     6301 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     6361 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     6421 cgactggcaa aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     6481 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     6541 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagcgg cgaaaaatat
     6601 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagctgaaca
     6661 acctcagccg caaaccgtgg atggcgttgc cagcccggca caagcctcgg ttagcgatct
     6721 gaccggtgaa cagcctgcgg cccagccggt gcctgtaagc gccccggcca caagcaccgc
     6781 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     6841 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     6901 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     6961 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     7021 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     7081 ggtgctgatc ccacgcagtt cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     7141 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     7201 cgcgggtgtt aacggcggtt ctggtatcgc tttaacgggt acaccgatta ctcccagagc
     7261 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     7321 ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac attgtggcaa cgccgggtga
     7381 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaatgct
     7441 gtacgccagc tcccgcagcg cacaaggcac cgcaggcccg gatttccgtc tggagatgga
     7501 aggtttgcag tacagcgaaa tcccgatgct ggcgggcggt aatctgccgt taatgcagca
     7561 ggcactcagc gcggtgaata acgattattc actggctcgc atgtatgcga tgggcgtcga
     7621 tgcctggtcg ctggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     7681 cggtaatacc ggaagcctga cggcaaaccc ggattgcgtg attaacagga agttatcatg
     7741 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     7801 gcgtgggaag cacaagcgct tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     7861 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggccg gaccaccgtt
     7921 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     7981 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     8041 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     8101 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     8161 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     8221 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     8281 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     8341 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     8401 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     8461 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     8521 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     8581 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     8641 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     8701 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     8761 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     8821 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     8881 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     8941 gattaagaaa gaagcgcgca ttaatgtaac ggcctatcag ggcaaagtgc tgctggttgg
     9001 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     9061 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     9121 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     9181 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     9241 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     9301 aaccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     9361 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     9421 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     9481 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     9541 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     9601 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     9661 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     9721 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta
     9781 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     9841 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
     9901 ccatttttgc accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat
     9961 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
    10021 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcatccc
    10081 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
    10141 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
    10201 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
    10261 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
    10321 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
    10381 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
    10441 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
    10501 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
    10561 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
    10621 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
    10681 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
    10741 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
    10801 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cccccatagc
    10861 actgaccacc agcatatgct gcgcgcccag ccgccgcccg gttaatgcgg tatccactac
    10921 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
    10981 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
    11041 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
    11101 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
    11161 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
    11221 cgctccccgg ctcatgcacc aggcttaata gaccatgagg taattatccc cgattgtgga
    11281 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
    11341 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
    11401 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaagga
    11461 gaagccagcg tgaccatcga taaatccatc gatgaagtga cgccagcgga gtttgatgcc
    11521 ctgttgctac cgggtggtca ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc
    11581 tttacccgtg attttgtgaa tagcggcaaa cctgttttcg ccatctgtca cggtccgcag
    11641 ctgctgatca gcgccgatgt gattcggggg cgcaaactga ctgcagttaa accgatcatt
    11701 attgatgtta aaaacgcggg cgcggaattt tacgatcagg aagtcgtggt cgataaagat
    11761 cagctggtta ccagccggac accggacgat ctgccagcgt ttaaccgcga ggcgttacgc
    11821 ctgctcggtg cctgagtcgc gtagccaaat cattttttta ccaaagccca gcgtgttgtc
    11881 ggtgaatttg atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac
    11941 cggaaagcga cgattgtacg cctggcgcgc gaggtcgctt tcctcacctt ccagcctgcg
    12001 gatctcacct ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac
    12061 cgttccggca accgcagcct gcggcccact catctgcgcg tggcgcgttt tttcttccgt
    12121 caaaatgtag aaggcgactt tctgcgcatc aaacagataa aaagcattag cgcaccacag
    12181 ttccccttcc tgctgcacac accaggtgac aacatgttgt tttgccaacc aacggctgat
    12241 ggtgatgagt gtttccattg ctgttctctt ttatactgtg ggcctgacct taacatgcga
    12301 cgcagacgat gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata
    12361 ccgggatcac cacggatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga
    12421 aagcactgcg cgggaaagga gaactgacgc tggcgttttc tgcgccagtg ggcgaccgtt
    12481 cgctggcact acgggcggaa tatcgcgtta agcaactgac caaacggcaa aaagagcgtc
    12541 tggtagcgga aggcgcaggg tttgcagaac tgttgagcag cctgcaaacc ccggagatta
    12601 aaagcgatta aaatgctcgt gatactcaac cagaccggtt acgccattca gcgcgtcatc
    12661 cgctaaacga tgtacctgga aggcgctttc agtgcccggc cagcggcaac gcagatcgtg
    12721 atgcgccgcc agttcaaagc cgaaacgact gtacagcgcc ggatcgccca gcgtcaccac
    12781 tgcggcatag ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag
    12841 cccctgtcca cggtattttt catcaaccgc caacggtgcc atgccgaccc attgcaggtc
    12901 ttcgccctgc acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc
    12961 tgtcgccacc agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc
    13021 ttccgcatca ctttcgaatg agcgacgcag cagggcatca atacccggcg catcaatggg
    13081 aatttctact cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt
    13141 ttcattcccg cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc
    13201 atttgctcca gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg
    13261 ccttcaatca ccagccgacg ctggaagaag agcgtatctg gatcttgttt acgcgccgca
    13321 atcatcagca gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggctg
    13381 accaccagtt tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga
    13441 atacttaacc agcggccttc aagaaactcc agctcgccat catccagcgc ctgacggaat
    13501 tgccagctta agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc
    13561 ggtacactca acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg
    13621 agttttactc cctgtttcaa caatcatcct attttgccat atcagaaaaa ttacatagcg
    13681 gtataaatca acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt
    13741 aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt
    13801 ttgatagcgc aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg
    13861 gcgcttaagg cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat
    13921 accaatgccc gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg
    13981 agttttgtcc atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg
    14041 gacggttacg cccgttggca gcgcgccgtg gatatggcgg cgcagctggg tgccgacgcg
    14101 ctgatcctcg ccgacctcgc catgctggaa tacgccgccg agcgttatcc gcatattgag
    14161 cgccacgtat cggtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc
    14221 cattttgacg ttgcccgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacag
    14281 ctggcacggg tcacacctgt accactggaa gtgtttgctt tcggcagcct gtgcattatg
    14341 tcggaaggcc gttgctatct gtcgtcgtat ctgacgggtg agtcgcctaa caccgtgggc
    14401 gcgtgttctc cggcccgttt cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc
    14461 ctgaacgaag tgctgatcga ccgttatcag gacggcgaaa acgcaggtta tccgacgctg
    14521 tgtaaagggc gttatctggt ggacggcgag cgctatcacg cgctggaaga accaaccagt
    14581 ctcaataccc tggaactgct gccggagtta atggcggcga atattgcttc ggtgaaaatt
    14641 gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct
    14701 atcgaccgtt gtaaggccga tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg
    14761 ctcgggtcga tgtccgaagg cacgcagacc acccttggcg cgtatcaccg taaatggcag
    14821 tgagaagagc aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagagacgc
    14881 tggaagattt ttatcagcag gccgccacca gcagcgccga cgtgatttat cttggtgaag
    14941 cggtatgcag caagcgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc
    15001 tcgccggaag tggcaagcag attgtgctct ccacgctggc gctggtgcag gcatcctctg
    15061 aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc
    15121 tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga
    15181 actgctacaa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta
    15241 tgccggtgga gctttcccgc gactggctgg tgaatctgct taatcagtgc gatgagctgg
    15301 gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctaccg ctggcctact
    15361 ccgctcgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct
    15421 gcattaagta tccgaacggg cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac
    15481 tcaatggcat tcagaccatg agcggctacg tttacaacct cggtaacgag ctgacatcca
    15541 tgcagggatt ggttgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcgatgc
    15601 tcgacgcctt ccgcgctaat gaaaatggcg ctgcgccact attgttgacg gcaaacagcg
    15661 actgtaacgg ctactggcgg cggctggccg ggctggagct gcaagcgtaa gtatatagct
    15721 cactttgtta acaactttaa ctactcttta atgcagtatt aaagactaat cggtaacaaa
    15781 gtgagctgtt atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccatccc
    15841 cgaaggttct tcagcgcggg aagcgttctc ccactctctc gacctcgccc gtctggctga
    15901 aaagcgcggc tatcatcgct actggctggc ggaacaccac aatatgaccg gcattgccag
    15961 tgctgcaaca tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg
    16021 gtctggcggc gtgatgttgc ctaaccactc accgttggtc gttgccgaac agttcggcac
    16081 gctcaatacg ctctatccgg ggcgaatcga tttggggctg ggtcgtgctc cgggtagcga
    16141 tcagcgaacc atgatggcgc tacgtcgtca tatgagcggc gatattgata atttcccccg
    16201 cgatgtcgcg gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc
    16261 ggtaccaggc tatggcgaaa aaatccccgt gtggttgtta ggctccagcc tttacagcgc
    16321 gcaactggcg gcgcagcttg gtctgccgtt tgcgtttgcc tcacacttcg cgccggatat
    16381 gttgttccag gcgctgcatc tttatcgcag caacttcaaa ccgtcggctc gactggaaaa
    16441 accatacgcg atggtgtgca tcaatattat cgccgccgat agcaaccgcg acgctgagtt
    16501 tctgtttacc tcaatgcagc aagcctttgt gaagttgcgc cgtggcgaaa ccgggcaact
    16561 gccgccaccg attcaaaata tggatcagtt ctggtcgccg tccgaacagt atggtgtgca
    16621 gcaggcgctg agtatgtcgt tggtaggtga taaagcgaaa gtgcgtcatg gcttgcagtc
    16681 gatcctgcgc gaaaccgacg ccgatgagat tatggtcaac gggcagattt tcgaccacca
    16741 ggcgcggctg cattcgtttg agctggcgat ggatgttaag gaagagttgt tgggatagtg
    16801 tgtcttaacg cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag
    16861 cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact
    16921 gatacaccgg cagtaaatta aagctcgata aaatatggac cagtgcgttg ccaacgccaa
    16981 acaccagaat cagcgtaatc atcggcttgc cgccccagac gcggaatttc gggctgccaa
    17041 agcgtttacg cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg
    17101 ctaaaccagc ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa
    17161 caggcggggc aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac
    17221 caaacagatc tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta
    17281 ccgcaaagtt tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc
    17341 ttaatgcctg taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg
    17401 gacgcgggat gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca
    17461 gcgtaccgta caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca
    17521 ggcttggcac gttaccgtga taaccaaacg acgccaggca gaacggcaat gtcatcagca
    17581 gatacggcgc ataagatgca ttgctttctg caacgttgaa caatgtcgca ggctgcacat
    17641 gccccaacag gctgccaaag gtgaggaaga aggtaatgac tttcgccccc agcacaatcg
    17701 ccgtcatgcg gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa
    17761 aaccaaaacc cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca
    17821 gaattgaacc actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg
    17881 aaataccgtt gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac
    17941 tcgaaccgat tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac
    18001 agaaccaggt aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca
    18061 ctggcagaga aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa
    18121 gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat gagggcttct ctccagtgaa
    18181 aaatagtgcg actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag
    18241 aaaaaaagcc ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc
    18301 tgttcagcga tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac
    18361 gcggagcgcg gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt
    18421 cacggcgttc accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac
    18481 caccgccacg acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca
    18541 tcggcttgtt gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt
    18601 tcggcagttc gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctgc
    18661 tgatgtcgcc ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc
    18721 ggcccacttc aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac
    18781 gatcttcacg gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac
    18841 ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt
    18901 caccctgtgc cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt
    18961 cagcagtcgg ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca
    19021 gctgctgctg tactttagcg gcgaattttt ccagacggcg tttgcctagc agttctgcgt
    19081 tcggcagttc tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac
    19141 gacgctcgcg gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac
    19201 gaccgatacg gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc
    19261 tgatacgctc aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac
    19321 gaccatcttt caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca
    19381 gcgcggcgct gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt
    19441 tggtacgaac gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg
    19501 cttcgttttt gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag
    19561 tcacgctgga ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac
    19621 gaatcgcttc cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg
    19681 ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca
    19741 gaaccagacc gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac
    19801 ggcccggagt accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat
    19861 aacgctggcc gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg
    19921 tcattgcttc agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg
    19981 gtgctttcag ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc
    20041 cgctccccgt ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact
    20101 ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag
    20161 ccttcaggcc cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca
    20221 aaattaatgg cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa
    20281 aagtgtgaac cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg
    20341 tgatggcaat caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc
    20401 tgccaggtca tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc
    20461 aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa
    20521 atcccccaga cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc
    20581 gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat
    20641 gttgcccagg tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa
    20701 gtgctgtttc aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag
    20761 ccacagacta cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg
    20821 cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt
    20881 gtaagttgga tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc
    20941 tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc
    21001 cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac
    21061 tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat
    21121 ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag
    21181 gacttcactt ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac
    21241 gaaacaccag cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg
    21301 tcctgttccc ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga
    21361 gggcaaatgg caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg
    21421 ttgagactgc tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc
    21481 cagaactttc accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg
    21541 tttgtcagcg atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa
    21601 tgcgccaaag tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc
    21661 tgcagtgatc tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc
    21721 gatcttcaca gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc
    21781 acggataaca gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat
    21841 ggtatggata cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc
    21901 cattacgccc aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat
    21961 ctctttggtg ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga
    22021 acctgcaact ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag
    22081 tacaacgtag ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt
    22141 gattggcaca cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga
    22201 agaaccgttg gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat
    22261 atccggcatg actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg
    22321 cttcggagaa ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa
    22381 caggaaggta tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt
    22441 acccagcgtt gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg
    22501 agtacgcggc agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc
    22561 gtcgatacgc ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg
    22621 cagaatttca cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat
    22681 ggtttcagat ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta
    22741 agcatcgctc aggcgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac
    22801 cggctccggc tgccagtccc aacgtggttt accggcttct ttcaccagtt cattgatgtt
    22861 ctgaataaca acctgctgtt gttcatgacc gaacactact gcgcccagca tctggtcttc
    22921 gctcagcagt tcagcttcag attcaaccat cagaacagcg gcttcagtac cggcaacaac
    22981 cagatccagt ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt
    23041 gatgtaacct acgcgagcag caccaatcgg accattgaac ggaataccag acagagacag
    23101 tgctgcggaa gcaccaatca tcgcgacgat atccgggtta acttgcgggt taacagaaac
    23161 cacggtggcg ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg
    23221 gcggtcaatc agacgcgcga tcagggtttc gccttcgctt gggcggcctt cacgacggaa
    23281 gaagctaccc gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg
    23341 gaagaagtcc tgacctggtt tggctttttt ctggccaaca acggtaacaa ataccgaggt
    23401 gtcatccatg ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag
    23461 agtcacggtg tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc
    23521 ctttctcttt cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat
    23581 cctcgcgact aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc
    23641 gcgaacctct gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc
    23701 tgccgtcagc ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta
    23761 gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag
    23821 gtagtcgagc agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg
    23881 atcttttttg tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc
    23941 tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt
    24001 agctgttgct tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac
    24061 gccgatctct aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg
    24121 acgttaagcg gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg
    24181 ccgggtattc aaccaccagg cgacgaggcg caacgcggcc ttcatcgtca atttcgccca
    24241 taccgataaa tttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac
    24301 cggatgtacg aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca
    24361 ccaccgggta gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg
    24421 cagccggaat atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca
    24481 ccatccgttc aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc
    24541 cacaaccgag tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc
    24601 agtgaatttc cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg
    24661 taatcgggcg cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt
    24721 taccctgata tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag
    24781 tatccagcgc cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt
    24841 gtccgtcggc atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt
    24901 tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca
    24961 tgccagttgc cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc
    25021 gtttcacttt ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa
    25081 cgccgttaat gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc
    25141 cgggttaaca cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg
    25201 catcccttca accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg
    25261 catcgctttc cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc
    25321 cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc
    25381 caggtcgcga gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt
    25441 aatttcacgc tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg
    25501 gcgaccaaat tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt
    25561 tttaaaatta atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt
    25621 cacatcaggc aacggctgcc ggatgcggcg taaaagcctt atccggccta caacccagac
    25681 aaccttaagc gatggtacgt tggatttcga tgatctcgaa tacttcgatc acatcgccag
    25741 tgcggacatc gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt
    25801 taacgtcatc tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt
    25861 cgcgcagaac gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacaacctg
    25921 caatggcacc aaatttcggt gatttgaata cgtcacgaac ttccgccaga ccgataatct
    25981 gttgtttcag ttccggagac agcataccgc tcattgccgc tttcacttcg tcgatcaggt
    26041 tatagatgac ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag
    26101 aggcatcagc acgtacatta aagccaacca ggatggcgtt ggacgccgca gccagggtgg
    26161 cgtcggtttc ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag
    26221 tagacagttt cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca
    26281 ggacaatgtt cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt
    26341 tagatttctg ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa
    26401 cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc
    26461 cggacaggcc gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca
    26521 gttcgttacg catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct
    26581 tgtgcagagt accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga
    26641 aggattcgat aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt
    26701 ccgcctgcag caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata
    26761 cgtgtacgaa ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt
    26821 cgttcttaac acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca
    26881 ccggtacctg cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt
    26941 cgtcggcagc aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg
    27001 aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa
    27061 cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg
    27121 aacgaatgta gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa
    27181 ccggcgcgcg cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca
    27241 gctcgttttc acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg
    27301 tttcctgatc gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga
    27361 tgacctgaga gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc
    27421 cgatcacaac gtcacggtta acggcctgag caggcttctg gaagccttgc tgcagggaag
    27481 aacctttacg ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag
    27541 cttttgattc agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac
    27601 ggccacggcc gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag
    27661 aagtagtgac gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt
    27721 tttcttccgc catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac
    27781 gacgcgcttc ttcttcagct ttacgcttga gctctgcagc ttcctgctca cggcgggctt
    27841 tttcagcctg ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg
    27901 cagcttcacg tttcgcttgt tcagcagctt cacgcttagc ttgttctgcg gcctcacgtt
    27961 cagctttttg ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt
    28021 ccgcttcacg ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt
    28081 tcacaaaggt gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac
    28141 caggaatgtt aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat
    28201 ttttctgatt caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag
    28261 cagacttccg gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc
    28321 gctctgcggc cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt
    28381 attacgcttc gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggctt
    28441 tttcgtcggt caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt
    28501 ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa
    28561 ggttcagcag atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa
    28621 tggtggccag tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc
    28681 cttcgatttc caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaaac
    28741 cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt
    28801 cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcattacgt
    28861 taagttccca accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg
    28921 cctgcgccag gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca
    28981 cgatagaagc aacgtctgcc ggtgccattg cgttaatcac aaactgcgcc gggttatcat
    29041 cccacaggac gatatcgata cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg
    29101 cgccacgcat acctacacag gcacctaccg gatcgatacg tttatcgttg gttttcaccg
    29161 cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc
    29221 cgatttctgg cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga
    29281 cgaacagttg cgcgccacgc gcttccgggc gaacggaata gagcacgcca cgaacgcggt
    29341 cgccagggcg gaagttttca cgcggcagca tatcttcgcg caggatcacg gcttcagcat
    29401 tgttgcccag atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga
    29461 tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt
    29521 tctgcacgat aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa
    29581 tctgatcttc aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa
    29641 gggtgatttc cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt
    29701 caaaatcacc gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt
    29761 tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg
    29821 ccttttcatt ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt
    29881 cacctcatcc agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg
    29941 cgaacacttc atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga
    30001 taacgccctg ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt
    30061 ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg
    30121 agacttccag gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct
    30181 ggtggctcac atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc
    30241 gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct
    30301 caactggcgc agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca
    30361 agacataaaa aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga
    30421 taaatcgggg ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc
    30481 agaaggattt tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag
    30541 gactctaagg gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag
    30601 cccgacgagc taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt
    30661 tgggtaaaag atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc
    30721 aggcattata gtgatccatg ccacattttg tcaacgttta ttgctattca cgtgaatgaa
    30781 tatccagttc actttcattt tttgaatact tttgccttct cctgctctcc cttaagcgca
    30841 ttattttaca aaaaacacac taaactcttc ctgtctccga taaaagatga ttaaatgaaa
    30901 actcatttat tttgcataaa aattcagtga gagcggaaat ccaggctcat catcagttaa
    30961 ttaagcaggg tgttatttta tgacgacgat tctcaagcat ctcccggtag gtcaacgtat
    31021 tggtatcgct ttttccggcg gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa
    31081 gggagcggtt ccttatgcat atactgcaaa cctgggccag ccagacgaag aggattatga
    31141 tgcgatccct cgtcgtgcca tggaatacgg cgcggagaac gcacgtctga tcgactgccg
    31201 caaacaactg gtggccgaag gtattgccgc tattcagtgt ggcgcatttc ataacaccac
    31261 cggcggcctg acctatttca acacgacgcc gctgggccgc gccgtgactg gtaccatgct
    31321 ggttgctgcg atgaaagaag atggcgtgaa tatctggggt gacggtagca cctataaagg
    31381 aaacgatatc gaacgtttct atcgttatgg tctgctgacc aatgctgaac tgcagattta
    31441 caaaccgtgg cttgatactg actttattga tgaactgggc ggccgtcatg agatgtctga
    31501 atttatgatt gcctgcggtt tcgactacaa aatgtctgtc gaaaaagcct actccacaga
    31561 ctccaacatg cttggtgcaa cgcacgaagc gaaggatctg gaatacctca actccagcgt
    31621 caaaatcgtc aacccaatta tgggcgtgaa attctgggat gagagcgtga agatcccgac
    31681 agaagaagtc acagtacgct ttgagcaagg tcatccggta gcgctgaacg gtaaaacctt
    31741 tagcgacgac gtagaaatga tgctggaagc taaccgcatc ggcggtcgtc acggcctggg
    31801 catgagcgac cagattgaaa accgtatcat cgaagcgaaa agccgtggta tttacgaggc
    31861 tccggggatg gcactgctac atatcgctta tgaacgtctg ttgaccggta ttcacaacga
    31921 agacaccatt gagcagtatc atgcacatgg tcgtcagttg ggccgtctgc tgtaccaggg
    31981 gcgttggttt gattcccagg cgctgatgct gcgtgactct ctgcaacgct gggttgccag
    32041 ccagatcact ggtgaagtta ccctggagct gcgccgtggg aacgattatt caatcctgaa
    32101 taccgtctca gagaacctga cctacaagcc agagcgtctg acgatggaaa aaggcgactc
    32161 ggtgttctcg ccagatgatc gtattggtca attgaccatg cgtaatctgg atatcactga
    32221 tacccgagag aaactttttg gttatgccaa aactggcctg ctctcctcct ctgccacttc
    32281 aggcgtgccg caggtggaga atatggaaaa caaaggccag taattcgaca tcaaccctgc
    32341 ccttcagggg tggggttacc ttgcttatcc tctgtaaact cctttctgag catcacataa
    32401 tcgaaaacat catggtttgc atccacaacc cgtgagtctc ctttatagtg tgcaatcact
    32461 tcctcaagcg tttgcggctg ttccggcttt gttaccccca tccacgcatt aataaggtaa
    32521 ttattgtaag ctgtcgaaaa gatgttgttt tccgttgtgc gatccacgcc atccccaaga
    32581 acggggctat accagataaa catcgggaca tgatatgcct gctggctagc ctccctgcct
    32641 ccatgaaaat agacgttctt cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac
    32701 atgaccgagg cgcggcgatc ttttaataat tcaaaaacct gacctagcaa actatctgtg
    32761 taatgaatgg agttgtcata gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg
    32821 gattgcggat aggcgctaca agccggttca tggcttccat ttaaatgaag aacaatcagt
    32881 ttcttttgct gcgtattttg ctgtaatgct tgcgataaat gcggcaacaa caattcatca
    32941 aatcctctga cataaactgt ttccatggcg cgcatggcga tactggtaac tgctgtacca
    33001 ttctgccgaa aagcggattg cgagcttagc cagaaagtct gaaatcctgc ttgattagcc
    33061 atattaataa tgttgtcggg gtaattatga atgtcatgac tcaaaacaga atcagcagtt
    33121 aaagaaaggg gaaccgacag cgcagtgtaa ggtgcgccgc ttattgcttg attaaacagt
    33181 ttgatctgtt ttctttgtgc ttcaacttgc ggtgtcgtag agcgtgtata tccatacaaa
    33241 gacatattgt cgacacgtac agactccccc acaatcaaca cgtaggtatc aatacctgta
    33301 tccctgactg ataattgaaa atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc
    33361 tctttcgctg ctaaagcaaa atagttgaga ttgaaaaacg gcgtataggt agcaaatcgc
    33421 gacgctagta tatatggact gaacgctttt ttattttttg catctttata agcaaattga
    33481 caagcggaaa ataaactgcc cgaaatgaca atcagcaata atattcctgt cacttttttt
    33541 gtcggcaagg aaacatcata ttttattatt actgccaaaa aaagaaggga taaaaaagca
    33601 aaggcacata gataagggat atacatcccc agcattttga caacttcatc cggatcactc
    33661 tggagcacac taatcgcaaa tccatcattg aaagttgtac caaaggtaca ccatgaataa
    33721 aggctaatac tcatgtctgc cgcagtcagt aacacaaatg gtgcggcaac aagcaggcga
    33781 agcggaaggg atgatatacg ttttacaacc aggatggtca ggacgaagaa cagcaatcgc
    33841 tgaaccatat gcgcgccgga agaggcgact aagttcgtaa taccaaaaac aataacacaa
    33901 agatacaaca accaatgaga tttaaaactt ctagcaaatt tgttgaatac tgtcatccag
    33961 aaatccaagt tcacaaccaa tataaacaat tatcaacaat gttcaatttt atatcagaca
    34021 tgtttttcaa ctctgatcca tatcagaccg aaacactgtt agttttttct ttttaaatga
    34081 tgcggccatt gcgttctcag taattaaata acattatcaa tgcgtctcta aacattacgc
    34141 agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga gaagtaaaca
    34201 tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat gtcagcagaa
    34261 gggaaaagtt tataactaag gcgtaccggc accatcgttt caaggtacca gctacgagta
    34321 aagcaactgg acgagataca gatacctgac aaccattcct cagaccaggg ccaaaacgaa
    34381 aaaagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg acttgaaccc
    34441 gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc accacctcgg
    34501 cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag cagctggttg
    34561 agtttgttcg gttttcaccg gtgcactcag attttcccat tcgctacctt tattggtttt
    34621 gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg ccagcagcgc
    34681 cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag cggaagcgcc
    34741 tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga tcagaccaac
    34801 aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa tcaacctgtt
    34861 ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt ttaatctttc
    34921 ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg caattttatc
    34981 gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgctgag aaaaaaggcg
    35041 acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac tgcatcggcg
    35101 atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt
    35161 aacggttcgg tgccggattt acgcagcaac acgcgtccac ggttgcccag cgcagcttca
    35221 acctctgcgg tcacggcttt aactgactca tgctcaagtg gatcgccgct acctgcggtg
    35281 taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg
    35341 ctcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat gccgtcaccg
    35401 gtagtagttt tatccagtag gatcacatga ccggaattct ctgcaccgat acgccagcct
    35461 ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt
    35521 cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc cacagcgcca
    35581 ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat ctgatcgcca
    35641 tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccatcgcc gtcgaaggca
    35701 ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc
    35761 cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat aacgttcgcc
    35821 cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc acaatccacc
    35881 acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca aaactcgata
    35941 tagcgacccg cggcatcaac gatacggctg gctttaccca gttctgccga atcaacgcag
    36001 ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg cagtttggtg
    36061 ccgtcgatag agaagaattt aatgccatta tcgtagaacg ggttatgcga tgcagatatc
    36121 acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg tgttggcatc
    36181 gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag tgctgactcc
    36241 agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga gccgtggcgc
    36301 gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca
    36361 tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact catagcgttt
    36421 gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg tttctttgac
    36481 gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac aggccagact
    36541 accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg attttcgtga
    36601 catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga
    36661 atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca atttctcttt
    36721 tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat tcacttctgc
    36781 aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct gcatatgcat
    36841 cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt cggaaaggga
    36901 gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga cttctggttt
    36961 ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa ccacaggaat
    37021 aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg tggactcgcc
    37081 accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc
    37141 tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt tgaggatccc
    37201 cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt tcatgatgtt
    37261 atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa
    37321 atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat ggtgttaccc
    37381 gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc attgtcgcca
    37441 gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc
    37501 atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc atctttcatc
    37561 gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata gttacgctca
    37621 atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat atgtttcgct
    37681 ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag cagtggaccc
    37741 aatttctcag agaagcccca ctgggtcacc atgttacgtg ccagattggt cgcaacttta
    37801 atatcgttgg acgcaccggt agaaacatgt tccggcccat agatgatctc ttctgccaga
    37861 cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc gctgattgcg
    37921 tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat aatcgtcact
    37981 ttgtgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg
    38041 taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc cgcacccatc
    38101 atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt gttgccacga
    38161 gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga gaaaccagga
    38221 gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg acgcatgtga
    38281 actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac ctgacggtcg
    38341 aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt cgcggcgata
    38401 acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg gttcagagtc
    38461 tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc tacggcgtcg
    38521 atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa catgtcacga
    38581 acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga gatagtaaag
    38641 aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc ggtacccgga
    38701 ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa gcggctcggc
    38761 tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg
    38821 tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac
    38881 gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat ccagacacca
    38941 atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct tggttcttca
    39001 ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa tttcggatcc
    39061 tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt gatttcacgt
    39121 ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa ggtagagtaa
    39181 tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga catcagcaca
    39241 acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg attaacctct
    39301 tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct ttcaaacttt
    39361 cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac ttcccgcgaa
    39421 cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga gcgaatttcc
    39481 cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact gccacctggc
    39541 gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc acgggggata
    39601 tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac ctggactttg
    39661 ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa atcgccctga
    39721 agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat gatgcggcct
    39781 ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc accaaggtcg
    39841 acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc aagtttaaac
    39901 caggcacggg aacgtaaccc ctttttctgt gcctgttgaa catatttatc gctaaagtgt
    39961 tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta actttcccat
    40021 ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg tgatatatgg
    40081 gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct
    40141 gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc cagttgttct
    40201 gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag cgttagagca
    40261 ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaactaaaa ccttgatcgt
    40321 ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta aaacgctggt
    40381 gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt
    40441 tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca aaatgccacg
    40501 ctctgttcgt tgataaaagg ccgcatagcg gcctttttcc tttctttaca atacatcaac
    40561 atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa attctacttc
    40621 gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca ggccacgagc
    40681 aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat cgccaacgat
    40741 gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag caccaaaaat
    40801 aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg acagcttggc
    40861 ttcgatgtct ttaatacggc cttcgcagaa accctgctgt tcacgagctg cgtggtattc
    40921 ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa tgatttcagg
    40981 acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt
    41041 catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc aagtcttcac
    41101 ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa gcaaaaaaat
    41161 accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatagt cattttaccc
    41221 tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt atgacggctc
    41281 gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg gattgaccag
    41341 ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc aactccccgc
    41401 tggtgccaac cttgccctga tggtgcaaaa agtcggcgcg tcggcccccg ctattgatta
    41461 ccacagtcag cagatggcgc tgcccgccag tacgcagaaa gtgattactg cgctggcggc
    41521 gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca aaggcaatgt
    41581 ggaaaacggc gtacttaagg gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa
    41641 acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga
    41701 tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc ccggctggcc
    41761 atggaatgac atgacacaat gctttagcgc tccgcctgcc gctgccatag ttgaccgcaa
    41821 ctgtttctcc gtctcgctct acagtgcccc aaaacctggt gatatggctt ttatacgcgt
    41881 ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc gaggttctgc
    41941 cgaagcgcaa tactgcgaac tggatgtcgt accaggcgac ctgaaccgct ttacgctgac
    42001 gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttgccgtgc aggatggagc
    42061 cagctatgcc ggggcaattc tgaaagatga gttaaaacag gcaggtatca cctggagcgg
    42121 aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca gtaaacagtc
    42181 ggccccgttg cacgatctgc ttaagattat gctgaaaaag tcggacaaca tgatcgccga
    42241 tacggttttc cgcatgatag gccatgcgcg cttcaatgtg cctggaacat ggcgggccgg
    42301 gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg gaaacaccat
    42361 tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca ccatgatgca
    42421 ggtgttgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca tgctgccgct
    42481 ggcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg
    42541 aaaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg gattcattac
    42601 cacagcgagc gggcaacgaa tggcgtttgt acaatatctt tctggctatg cagtagaacc
    42661 ggcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt tgtataaaga
    42721 tatttatcag aacaattagt caaaaagaaa ccccggcaca tggccggggt tttgctgatt
    42781 aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtca tcatcccagt
    42841 cctcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatca tcccacatga
    42901 attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt tcaatgataa
    42961 aggtcatcac atcccagcag agatctttca cgcccagtcc actcgccgca gagatcagat
    43021 aatatttatc ttcccagccc agcgcttcag caatcgcttt cgctttctct tcagcttctg
    43081 ccttatccag cagatcgatc ttattgaaca ctaaccaacg cggtttcgcc gccagatcct
    43141 ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt
    43201 caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg tgcttcagga
    43261 agcggatacc cagacctgcg ccttccgcag cgccttcaat cagtcctgga atatcggcaa
    43321 caacgaagct cttttcgttg tccattcgta ccacacccag acttggcacc agagtggtaa
    43381 acggataatc cgccactttc ggtttagccg ccgataccgc gcgaataaag gtcgatttac
    43441 ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc agcagcagct
    43501 cacgcttatc acccggcgta ccgttggttt tctgccgtgg tgtacggtta acggacgatt
    43561 tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt
    43621 gtttagtcat atcgcccatg gtttcaccag taccctggtc gattacacgc gtacctaccg
    43681 gcactttaat cgtcacgtct ttaccgcgct taccggtaca atcgcggctt gcgccgttct
    43741 gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg ttcaggtttt
    43801 cgtcggcttc catccatacg tcaccaccat caccaccgtc gccgccatcc gggccgcctt
    43861 tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca cctgcaacga
    43921 ccagaatcga tgcttcatca acaaacttca ttttattctc cgtaaatcat tcgcctgcgc
    43981 ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga tgaccaatgg
    44041 cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa aggtttaaca
    44101 tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa
    44161 acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga gccatcgctt
    44221 ccgccagggc gccatatcct accaatgtat tcagtccgca aaaaattaaa catgcgagct
    44281 gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc gcaattgtac
    44341 ataaagtgta cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat aaaacctttt
    44401 gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac cccaaagata actccccagg
    44461 tgtaatcggt gagctttgta aatatctcga ccagactggt gttaaaaaac atcaccaggc
    44521 cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct ttcaggataa
    44581 atacgctggc aaccatcata ccaactggcg agagttgccc aatcacctgc gaagcggtcg
    44641 gactcaggta ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg
    44701 ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc ggcaacctct
    44761 tcttcaccgc aagaatggca ccaaggccaa tactcgccat caagaaacgg taaaacacga
    44821 ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac gccccccagc
    44881 aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt
    44941 ccctgcaaag cgcatttgcg gattaacgtc gcaccatgcg catttttacc ggttatcgaa
    45001 tgtaaaaagc cccacaacgt gttgcggggc tttcatccgt taccgggacg cgaaaaactt
    45061 attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg aatttcactt
    45121 taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta gcgccagcgt
    45181 ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat tcgccaccga
    45241 aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg gagccgccag
    45301 cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc cagtaatttt
    45361 cacatcagtg aaccactgac gatggccctg ctgcttacga tagtgtttac gacgacgaaa
    45421 cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt tgattacgcc
    45481 gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca tcagcacttc
    45541 agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa cggtctgacc
    45601 ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat aaaactccgc
    45661 ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa tagggcgcga
    45721 atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa gaaaaaaaac
    45781 acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc ctatattcct
    45841 aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa aagcccggct
    45901 tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc gggtgttaat
    45961 gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt aggctattac
    46021 atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc tgcacgagct
    46081 gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt tatccacacg
    46141 gcgactctgc tacacgacga cgttgtggat gaatcagata tgcgcagggg taaagctacc
    46201 gccaacgctg catttggcaa tgccgccagc gtgctggtag gcgattttat ttatacccgc
    46261 gcgttccaga tgatgaccag cctcggttcg ctcaaagtgc tggaagtcat gtcagaagcc
    46321 gtaaacgtca tcgcagaagg tgaagttctg caactgatga acgttaacga tccggacatc
    46381 actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc
    46441 gcgcagtgtt ccgggattct ggctggctgt acgccggagg aggagaaagg cctgcaggat
    46501 tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga ttacaatgcc
    46561 gatggcgaac agttaggtaa aaatgtcggc gacgatctga acgaaggtaa accgacgctg
    46621 ccgctgctgc acgcgatgca tcatggcaca ccagaacagg cacagatgat ccgtaccgcc
    46681 atcgaacagg gtaacggtcg ccatcttctg gaaccggttc tggaagcaat gaacgcttgt
    46741 ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc catcgcagcg
    46801 ttacaggtgc tcccggacac cccttggcga gaagcactca tcggcctcgc gcacatcgct
    46861 gttcaacgcg atcgttaatc ccctcccctc atcccgcgca gcgcgcggga tggttccagt
    46921 aaattccata aaaacactta ttcagctcta taacacccga aaaacagggc tgtgcatctt
    46981 tatatattct gaatattcac actctttaca ggaacttttt agagcaatag gccatcagga
    47041 gtatagtgat gctcgacaga agaagtgttc tgaatgaaag cgactaactt aaggagtgag
    47101 gaaagatgga aagtaatttc attgactggc atcccgctga catcattgcg ggtttgcgca
    47161 agaagggaac gtcaatggcg gcggagtctc gcagaaatgg tttgagttcc tcaacgctgg
    47221 caaacgcatt atcgcgccca tggccgaaag gagagatgat tattgcgaaa gccctgggaa
    47281 ctgacccctg ggttatctgg ccatcacgct accatgatcc gcagacccat gagtttatcg
    47341 acagaacgca gttgatgcgt agctacacta aaccgaaaaa atgagtggtc tggcggtagc
    47401 cccgcgaacg gggctgccag ctctcagacg attactcgcc tttcacacgc tcaatatttg
    47461 cacctaaagc gcgcagtttg tcttcaatgc gttcgtagcc acgatcgatg tgataaatac
    47521 gatcaaccac cgtcgtccct tccgcaatac aaccagccag caccaggctt gctgatgcac
    47581 gcagatcggt tgccataacc tgtgcgccag aaagtttttc aacaccgtga caaataacgg
    47641 tattgctttc gatttcggca tgcgcgccca tacggctaag ctccggcaca tgcataaagc
    47701 ggttttcaaa gaccgtttcg gtgatgaatc cggtcccttc tgccaccagg ttcaacagcg
    47761 tgaactgggc ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt acgttaacag
    47821 ccttcggacg tttgccatgc atatccaggc taatccagtc ttcgccgact tcgatgtccg
    47881 ctccagcgtc acgcagtttc gccagcacgg cgtcgagagt atctggctgc gcgttacggc
    47941 agataatttt gccgcgagaa atcgccgccg ccaccaggaa agtaccggtt tcgatacgat
    48001 ccggtagcac gcgatagaca ccgccgccta aacgttccac accttcgatg acgatacgat
    48061 cggtgccctg accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg gtatcgacga
    48121 tttccggttc acgcgctgcg ttttcaataa tcgtggtacc ttccgccagg gttgcagcac
    48181 acatgatggt caccgttgcg ccaacgctga ctttatccat cacgatatgt gcgcctttca
    48241 gacgaccatc gacggaagct ttaacgtaac cttcttccag tttgatggtc gcgcctaatt
    48301 gttcgaggcc agaaatgtgt agatcaaccg gacgcgcacc gatcgtacaa ccgccaggca
    48361 gtgaaacttg cccctgacca aagcgcgcta ccagcggccc cagtgcccag atagaagcac
    48421 gcatggtttt aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg gcatcaatat
    48481 gcacagaacc attacgttct actttcgcac ccagctggct tagcagcttc attgatgtat
    48541 cgacgtcttt cagttttggg acgttctgga tctctaccgg ttcttccgcc agtagagcgg
    48601 caaaaaggat aggcagagca gcatttttag cgccggaaat tgtgacttcg ccctggagct
    48661 tcgttggccc ctgaacacga aatttatcca tttagtttgt tctcagttaa caattcatat
    48721 ccgctaccgg cgaatcgccc atagctcaaa agccgttcag tttgcgatcg cgcgcccact
    48781 ccgcaggggt atacgctttg atcgacacag catgaatgcg gttatccgca atatattcca
    48841 tcagcgggcc atagaccgtc tgctgttttt taacccgact catgccgtca aacaactcac
    48901 ccacggcaat aacctgaaag tggctgccat cgccggaaac gtggacttcc tggagggaga
    48961 gagcgttcat caacaagctc tgaatttcat tattttccat gggatcttca atcatcagtt
    49021 aataaaccag cgaaacatct tagagcaaag ttgcgctggc ataaataagc aaaaagcctc
    49081 gctgataaat cagacaaggc tcgacttgca ggcaggtttg ccggacaggc ggttaacgcc
    49141 atatccggcc tgaaaaaatt taacgaggca gaacatcagc aggcaaatta tacaatttcg
    49201 cgagggtata cactttgtcg tttaccccct gaagcgtcac gttgttgccc tgctttttcg
    49261 ccagatcgat aagatggagc agcagtgcca gtccccccgt atccacgcgg gacacacggc
    49321 taagatcgat gcaggtaatc cccttcaccg cttcctcacg catttcccaa agcggtagca
    49381 gaacgtcctg atccagctct ccgaataacg ccagcgtgtc acccgtctgc atccagctca
    49441 gtgactcgct cattattttt tctcttccag agtgattttc tgttgagaaa tcgatttcag
    49501 ttgcgcagtc aggccgtcga tacctttggt acgcagcagc gttccccact cgttttgttt
    49561 ggtggtgatc atactgacgc cttcagcaat catgtcgtaa gcctgccagt ttcccgtctg
    49621 ggagttttta cgccactgga agtccagacg caccggcgga cggccattcg ggtcaataat
    49681 ggtaacgcga ataggcacaa tggttttatc gcccagcggc tgttctggcg caatctgata
    49741 ggtttgaccg tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt actcacggaa
    49801 agcggcaaag taggcttcac gttgagcagg ggtcgcactc ttgtaatact ggcccagcac
    49861 cagcgcaccg gcgtatttca cctgtacgta tggcagcagt tcctgatcaa caatggtacg
    49921 cagataatcc gggttggccc gaatttgcgg ttgctcattc ttcaggcgat cgaacgtttt
    49981 ctgcgccgcc tcgtccatca gcttataagg attggtctgg tctgccgcgg ttgccgcact
    50041 cagaggtgca atcaccagca aagcgaccat cattaaacgt ttaaacatgc gtcggttctc
    50101 ctgaaattat ttcgttgtac ccacaggttc agtggtttca ttattacctg gcgcagcagc
    50161 tggcgcatcg ccactattct tattgtcatc gcctttacta ccgtaaagga actgaccaat
    50221 gagatcttcc agcaccatcg cagacttagt gtcctgaatt gtatcgccat ccttcaggat
    50281 agcagtcccc agttccgggt cttcaaaacc gacgtttaat gccagatatt gttcccccag
    50341 caggccggaa gtacgaatgc tcagcgaact ggtatctgga atgtggttat aacgttgttc
    50401 aatttccagc gttacgcgcg gcagataggt tttcgggtcc agcgtaatat ccgccacccg
    50461 acccacaaca acgccaccaa tactgaccgg agagcgcgct ttcaggccgc caatgttatc
    50521 gaacgtcgca taaagcgtgt aggtcggttc agtacgtatg gacgtcacgt tcgccgcctt
    50581 caggcaaaca aacagcgccg ccagcagtgc tgctaataaa aagataccca cccaaatttc
    50641 attttttttc gtttgcatga actcaattcc caaacatcaa tgcggtcagc acaaaatcca
    50701 gccccagaac agccagagac gagtggacaa cggtgcgagt ggttgcccgg ctaatcccgg
    50761 cagacgtcgg gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc gtgatggcga
    50821 acaccacgct cttaatcaga cagttgacca gatccatacg ccagtcgacg gcattttgca
    50881 ttgccgacca gaagaacccg ctatcaatgc ctttccaact gacgccgacc agagatccgc
    50941 cccagatccc cacggcgacg aaaataaccg tcaacagtgg taatgaaata accccagccc
    51001 agaaacgggg agaaataacc cgacgcagcg gatccaccgc catcatctcc atactggaga
    51061 gttgctctgt agcgcgcatc aggccgattt ctgcggttag cgccgaacca gcacgcccgg
    51121 caaacaacaa cgcggcaacc accggcccca gttcacgcag tagcgataac gccaccagca
    51181 tacccagact ggtttccgca ctataagtgg tcagaaccag ataaccttgc agccccaaca
    51241 ccattccgat gaacacgcca gaaaccacaa taatcagcat cgacaggacg ccgacattat
    51301 agagctggcg caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg accagcgcat
    51361 tgaataacat taacccggcc cgcccgaacg ttctcagggt tttaatccct ttatgtccga
    51421 gcgacgccag cgcatttaac agcatgagtg gcttaactcc ctggtaaaag atcagcgtga
    51481 taatcgccgg caggatagcg gaacggaaca ggcccgtcag ctatcccgtc cagaaactga
    51541 cgtacgcgcg gatcaggatt cgcctgcaac gcctgggcac tgccatgagc gacaattttt
    51601 ttgtccgcca ggatccaggc gtgatccgca atacttaaca cttccggcac atcgtgagaa
    51661 accaccacac aagtcacgcc cagcgcgctg ttcagctcag aaatcagctt caccagtacg
    51721 cccatggtga tgggatcttg cccaacaaaa ggttcatcaa acatgatgag atccggctcc
    51781 agcgcaatcg cacgcgccag cgctgcacgc cgcgccatcc caccggaaag ttcagaaggc
    51841 attagtttag ccgctccacg cagccccacg gcctccagct tcatcatcac cgtactatgc
    51901 aacaatggcg cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt gtcaaatacg
    51961 ttcatatcag tgaacaacgc cccggactga aataacatgc tcatccgttt gcgcactgta
    52021 tacaggcgcg aacgagacat cgccggaata ttctcaccat cgaaaaggat ctcaccatga
    52081 tctggtgcga tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat gcccgatggc
    52141 cccatgatcg ccgtgatctt ccctcgcggc acggtcaggg aaatattatc gaagatgcag
    52201 cgattgccac gcgtaaaact gacatcgcgc atatcgacta aattcgccac agactgctcc
    52261 ataattcacc cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt aaccttgaac
    52321 ccaacatatt tacagaatat tacccgccgt ggttagcgaa agctggcatt tgttttactt
    52381 tttagccgca taaagtcaaa attaagaatc cgttacggct ttctgaaaat cttcagcgga
    52441 ccggcgagta tacctgaaga aaggacgtta gatgctttta gctacggcac tgttaattgt
    52501 tggtttactt ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt ctattctttg
    52561 ccgaaccttt ggcatcccgc cgctgatcat cggcatgacg gtggtcagta ttggtacatc
    52621 gttaccagaa atcatcgtct cgcttgccgc gtctctgcac gaacaacgcg atttagccgt
    52681 tggtacagcc ctcggctcaa acattatcaa tattttgctg atcctcggtc tggctgcgct
    52741 ggttcgtcct tttaccgtcc attccgatgt tctgcgtcgt gaattaccct taatgttgtt
    52801 ggtcagcgta gtggccggtt ccgtactcta tgacggacaa cttagtcgca gcgatggtat
    52861 ctttctcctc tttctggctg tgctatggct gctgttcatt gttaaacttg cacgtcaggc
    52921 tgaacgtcag gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg
    52981 cggattgccc gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac
    53041 gcggatggtg gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac
    53101 gatgggtctg acggcaattg ctatcggaac cagcctgccg gaactggcaa ccgcaatagc
    53161 gggggttcgc aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt
    53221 taatattgtc atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccatt
    53281 ggcgtacagt cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg
    53341 ctggcggcgc tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat
    53401 cgtatggctg gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca
    53461 ttatgtcgca cgtagagtta caaccgggtt ttgactttca gcaagtaggt aaagaagtcc
    53521 tggcgattga acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc
    53581 ttgcctgtga aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat
    53641 cggggcacat cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt
    53701 tcgtccatcc tggtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg
    53761 tgattgctat ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta
    53821 agcgtcttca cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg
    53881 ccgcagatgt gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac
    53941 cgaccagcag caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa
    54001 aagcacgcgg ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc
    54061 gtaaacttct gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta
    54121 agaaaacggc cagtctgcgt gacgcgttgc tggaagttac ccgcaaaaat cttggtatga
    54181 ctgtcatttg cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc
    54241 gtgtcttcga tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg
    54301 ggggaatacg tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc
    54361 gccatatcac ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc
    54421 atgatttact gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc
    54481 aggtgcgtcg cttgcgacct gttacggccc tgtcagcgcc gacgttatag caaaagcaga
    54541 gaacattcgt ctgctgatcc tcgatgtcga tggcgtactg tcagatggcc tgatttatat
    54601 gggcaataat ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg
    54661 tgcgctcacc tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga
    54721 agatcgttgt gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat
    54781 cgcctttagc gatctgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgtcgg
    54841 cgatgatctc atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga
    54901 tgcgcatcca ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg
    54961 cgcagtgcgc gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa
    55021 agggcaatcg atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt
    55081 ctggtgatga tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac
    55141 aacaatgatc ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg
    55201 gcactaagct atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg
    55261 tggtttacgc agccggtact taccacgttt gataaggata aaatcccgac atggtccgta
    55321 aaagcagata aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa
    55381 gtcaacgcac ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc
    55441 aatctggtga cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt
    55501 aactccagcg gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa
    55561 aaggttagaa catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc
    55621 cagctcactt ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc
    55681 gatccacatt gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac
    55741 cggtaatgtc atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac
    55801 ccgtccgggc ggcgaacaag gtaaagaagt gattgacggc tacggtaaac cggcaacgtt
    55861 ctaccagatg caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga
    55921 actggcaaaa gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa
    55981 cattaagggc gataagatca cttacctggt aaaagagcag aaaatgcagg ctttcagcga
    56041 caaaggcaag cgcgtaacca ccgttctggt gccatcgcag ctgcaggaca aaaacaacaa
    56101 aggccagacc ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa
    56161 gaaccttgca aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa
    56221 ctccggggaa attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta
    56281 catggttgta ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat
    56341 cagtctgctg cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc
    56401 ctccattttc cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga
    56461 cgacttgtct gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat
    56521 tgagcacctg cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga
    56581 aattgcccgc gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg
    56641 ggttgacccg atctcggtta tcgacattaa acgcatcatt aagcacctgc gcgacagcgg
    56701 cctgggcgtg ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc
    56761 ttatatcgtc agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga
    56821 cgaacacgtt aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt
    56881 gcgacgtttt agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt
    56941 agccaacaac tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg
    57001 acgctggaac ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa
    57061 atcgacactc atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc
    57121 gccgacgcgc tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac
    57181 accatttaca ccgctggtac accatccggc accagcggtg actacattga cgatgagctg
    57241 ccggtctatc agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag
    57301 ctgacaccgt tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat
    57361 gacaccggtt atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag
    57421 atcgacatcg acgaggttga agccgtcctt aagcggatcc aacggtttga tccggtcggt
    57481 gtagcggcaa aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataaaacc
    57541 acgccgtggc tggaagaggc cagactgatc attagcgatc atctcgatct gttagccaat
    57601 cacgacttcc gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc
    57661 gtcaatctga tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct
    57721 gagtatgtca ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc
    57781 aacagtgaca gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac
    57841 gcgcgcaacg atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg
    57901 atcaagagtc tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa
    57961 cagcagcaag ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat
    58021 atcgcccagg ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac
    58081 ctgcatagtc cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc
    58141 gagggcggcg gcgaagcttc ctccacggcg attcgtgcgc tggtgaagaa attaatcgcg
    58201 gcggaaaacc cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa
    58261 ggtatcatgg tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg
    58321 tcaaaccagc gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa
    58381 cattaccgga aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt
    58441 tgccaaactt gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga
    58501 gaaagtcacc cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag
    58561 cgcggaaggt caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca
    58621 gttgaccaaa cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc
    58681 atggcggcct gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc
    58741 cactgtttga gtgggcaggt tcttaggtga aattatgaca aatgatgata caactctaca
    58801 gcttagcagt gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa
    58861 acgcgccctg gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt
    58921 ggtttttgaa gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat
    58981 tgccattccg catggcaaac tggaagaaga tactctgcgc gccgttggcg ttttcgttca
    59041 gctcgaaacg cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc
    59101 cctgctggtg ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa
    59161 acgtctggcg gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct
    59221 gtatcaaatc attacggata ccgaaggtac tccggatgaa gcttagttat tcggtaatgt
    59281 ctcttttaga cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc
    59341 aggttcaggt aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga
    59401 taaccttccc gtagtgttgt tacccgatct ggctcgaacc ctggccgatc gcgagatttc
    59461 tgccgccgtc agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc
    59521 gatgagtaac ctgcctgacg ctttctcacc gcaactcctg ttcctggatg ccgaccgtaa
    59581 taccttaatt cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct
    59641 gtcgctggaa agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc
    59701 ggatctgatt gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac
    59761 ccgtctgctg ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa
    59821 acacggtatc cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca
    59881 ctgggatccg aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga
    59941 ccgccacaca gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt
    60001 acctatgctg gaaaccaaca accgtagcta cttgacggtc gccattggtt gtaccggcgg
    60061 gaagcaccgt tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa
    60121 aaacgtccag tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact
    60181 gttgaaatca caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta
    60241 atgcagggtt ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggccgaagcc
    60301 aacagcgtta ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa
    60361 gcgaccggcc cacaggaaga ggaagcactg gccgccgtta tcgccctctt taactctggt
    60421 tttgatgaag attaatcttc atcactttga catacaactc ccttcaaact ccccccgaca
    60481 ataagaaaat cacgtactga aatcattctc aatcaacgtc atttgtacat tttgtgcgct
    60541 tttcactttc agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag
    60601 ttaatcgacc atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta
    60661 tctgaacaaa gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat
    60721 gcccaacgct gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc
    60781 cgtgagttaa agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg
    60841 cgctatcacg gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgca
    60901 gattttgacg gcaaaccctc cattacgctg accgaatttg ctgaacaatg ccgttacgaa
    60961 gaagacattg cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac
    61021 cgtatcgtga cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca
    61081 gaggttatcc cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc
    61141 tggtcgaagt ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa
    61201 ccggcgctgg ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc
    61261 ctcacttcgc gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac
    61321 tgatacatct gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag
    61381 actttgaaac gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta
    61441 agtttgctcg cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg
    61501 tcgccaaatt cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt
    61561 tctatcccca gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat
    61621 aaaaagagat ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca
    61681 ttgcgctcgt tgtgcgccag ggctttctca atggaagcga cattaaagcc ccagtgctca
    61741 agaaatttct gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc
    61801 atactgaccc agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc
    61861 tgtcgctcga ccattaccgc tgagaaggga acaggcgcaa cactaaacaa cgcgatgccc
    61921 ccgccccaga aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta
    61981 aacaccgtta agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat
    62041 caatgccgct cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa
    62101 tcttattgtc ttcatcaacc acgatatcat cgacaggaca gggcacatgc tccgcgccca
    62161 tctcttccag cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat
    62221 cgaaaatttt cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct
    62281 gatgcatcgc ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagag
    62341 tggcaaaatt gcttaaattc ttcgccgcgc caaccccccc cggcacaatc aacgcatcca
    62401 gttcagcggc atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt
    62461 caatcagcac gttgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa
    62521 cctgctgctt atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca
    62581 acgtcaacac cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta
    62641 cgccaatttt cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt
    62701 aataaaaatg gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg
    62761 ctatcttaac tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa
    62821 tttacggcgc agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc
    62881 cggggtcatt ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc
    62941 cactcttctt tcatctcttc aatccattca ccgacgttat cttcccaggc aggaaggtcc
    63001 ggagactgaa tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca
    63061 cctttaattt tatgtccttc ctctacaatg ccttttttat cctgcgccgt cagattcgac
    63121 tccagcacgc tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc
    63181 agcttcggtc ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct
    63241 gatttactgt tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat
    63301 ttcttgatca tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt
    63361 ccagcattga ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc
    63421 ggtaaatcct cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata
    63481 tctggcaact gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc
    63541 gccgccttgc cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc
    63601 gcaacaatca cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc
    63661 atatcgtctt catcaaacgc atcatcgacc tcttctgcta ccgacggtgc gtggatcgtc
    63721 aacgtaaagg ttgaaccttt gccctgttcg ctggtaaccg taatatcgcc gcccatattt
    63781 ttcgccagac gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca
    63841 tgactgtctt tcacctggta atacatggcg aaaattttat ccagctcatc ctgcggaatg
    63901 ccgataccag agtcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc
    63961 acggtaacct ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc
    64021 tgccgtaaac gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt cggctccagg
    64081 ttaaagcgca atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg
    64141 aagctggtga aatcaaccgg ttgattatca agctggacct tgcgccgttc catcttatcc
    64201 atgtcgataa tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatggtcttg
    64261 agatattttt cctgctcggc ggtgagttcg gtatccagca gaatgcggct cagaccgacg
    64321 ataccgttca gcggtgtacg caattcgtga ctgatggtgg agataaacgt cgttttgtcg
    64381 cggctggccc gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag
    64441 cccatcaaac cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag
    64501 caggctttgc gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga
    64561 cggaacactt tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca
    64621 gcaggtttca ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg
    64681 ttacagccgg aaaactcttt atcttcgtta cgataaaaaa ccaggtcggg tgaagcatca
    64741 aggaaggaac gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct
    64801 ttgatttcaa ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga
    64861 acggcaattt cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa
    64921 tcgcgctcgc gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc
    64981 tccagttgct cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca
    65041 aagaagatag aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc
    65101 gccatttgta ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc
    65161 accagaccta acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc
    65221 attagggaat tccttcacga caacctgccc caataatacc caattctgac agttacgtta
    65281 taaattgtgt gagaaatgca gaattcccct caacgaccta ctccgttgga ggtagccacg
    65341 gacgtcccag cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca
    65401 ttcccgtcca tcgattttcg caccacagag gagcaagcag cgtcgggcga cgggcgctgg
    65461 cggaaatgcg gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga
    65521 gcgtgtaatc ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa
    65581 tgctgccttt cacaatatgc agcggatgca gctttatgcc gtccacgccg gtttcaacca
    65641 ctcgctccag cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt
    65701 gggaacacac cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa
    65761 aatcatggcc gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct
    65821 ccagccacac ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg
    65881 gcacgcagtc cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct
    65941 gctgatacat agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt
    66001 aggccagata gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa
    66061 tggaacgatg ctgctgcgct tcatcggcaa acgaggccac attacagaat gtgcagccac
    66121 cacgcccgat ggtaccgtca cggttagggc agctaaaacc gccgtgcagc gtcagcttat
    66181 gcaccttttg cccataacga cgggtgagat caccaccaaa catattgact aatttctgta
    66241 actgcataat ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca
    66301 attcggcgat gacctggatc aatcgtcccg gcctgctttt atcaactgca taatcaatca
    66361 aaattaccga aatttcatgc ataatcacat aaatcacttt tgcttatctt gtgtcagatt
    66421 tttttatctc cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa
    66481 ggcagtataa aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt
    66541 tatgacagtc aggaattgac tgtttctcta acgacttccc ttttagcctt aaagataaaa
    66601 tccattttaa tttcagtcat ttaataaaga attttgtgct aaagcacatt tctgtaccaa
    66661 taagcttgcc atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt
    66721 tctggatgag cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta
    66781 ttaaccgatg cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaac
    66841 ccccgtcgcc acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag
    66901 agcctggggg aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt
    66961 ttcggcctga tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata
    67021 cacgcactgg cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggtgac
    67081 ggttgcggct tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc
    67141 ggctggcgtt tagcgaaaaa ctacgccgtc gggatgctat tcctgaataa agatcctgaa
    67201 ctcgccgctg ccgcacgccg catcgttgaa gaagaactgc aacgcgaaac cttgtcgatt
    67261 gtgggctggc gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct
    67321 ctgccacgca ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc gcgcgatatg
    67381 gagcgccgtc tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac
    67441 ttctacgtct gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg
    67501 gatctgccgc gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catctgcctg
    67561 ttccaccagc gcttctccac taacaccgta ccgcgctggc cgctggcgca gccgttccgc
    67621 tacctggcgc ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg
    67681 cgtacctata aattccagac gccgcttatc cctgatctgc acgacgccgc accgttcgtc
    67741 aatgaaaccg gctctgactc cagttcgatg gataacatgc tggaactgct gctggcaggc
    67801 ggaatggata tcatccgcgc catgcgtctg ttagtgccac ccgcctggca gaacaacccg
    67861 gatatggacc cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg
    67921 gatggcccgg cgggcatcgt gatgtccgac ggtcgttttg ccgcctgtaa cctcgaccgt
    67981 aacggtctgc gtccggcgcg ctacgtcatc accaaagata agctcatcac ctgcgcctct
    68041 gaagtcggta tctgggatta ccagcctgac gaagtggtcg aaaaaggccg cgtcgggcca
    68101 ggcgaactga tggttatcga cacccgcagt gggcgtattc tgcactcggc agaaaccgat
    68161 gacgatctga aaagccgcca tccatataaa gagtggatgg agaaaaacgt ccgccgactg
    68221 gtaccgtttg aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg
    68281 cttgccagct accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc
    68341 gtactgggcg aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc
    68401 gtgctctcca gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg
    68461 actaacccgc caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc
    68521 ggtcgtgaaa tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa
    68581 tcgccgattc tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac
    68641 cgcgcagata cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc
    68701 aaagagctgt gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgct gctggtgctc
    68761 tccgaccgga atatcgctaa agatcgcctg ccggttccag ccccgatggc ggttggcgcg
    68821 atccagaccc gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc
    68881 gccagcgccc gcgatccgca ccacttcgcc gtgttgctgg gcttcggcgc gacggctatt
    68941 tatccatacc ttgcctatga aacgctgggc cgcctggtag acacccatgc gattgccaaa
    69001 gattatcgta ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc
    69061 atgtccaaaa tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg
    69121 gtcggtctgc acgatgatgt agtgggcctg tgcttccagg gggcggtcag ccgcattggt
    69181 ggggcgagct ttgaagactt ccagcaggat ctgctgaacc tgtcgaaacg tgcctggctg
    69241 gcgcgtaagc ccatcagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac
    69301 gcttacaacc cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc
    69361 gactatcagg aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg
    69421 ctggcaatta cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa
    69481 ctgtttaaac gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac
    69541 gaggcgctgg cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc
    69601 gaagacccgg cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt
    69661 cgcttcggcg ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc
    69721 cagggcgcga agccgggcga aggcggtcag ttgccgggcg acaaagtcac tccttacatc
    69781 gccaaactgc gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat
    69841 atctactcta tcgaggactt agcgcagctc attttcgacc tcaagcaggt taacccgaaa
    69901 gcgatgatct ccgtgaagct ggtttccgaa ccgggcgtag gcaccatcgc gactggcgtg
    69961 gcaaaagctt atgccgattt aatcaccatc gcaggctatg acggtggcac cggcgcaagc
    70021 ccgctgtcgt cagtgaaata cgcaggttgc ccgtgggagc tggggcttgt tgaaacccag
    70081 caggcgctgg ttgccaatgg tctgcgtcat aagatccgtt tgcaggtcga tggcggcctg
    70141 aaaaccggtg ttgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc
    70201 actggcccga tggtggcgct cggctgtaaa tatctacgta tttgccatct gaacaactgc
    70261 gcaacgggtg tagcaactca ggatgacaaa ctgcgtaaga accactatca cggcctgccg
    70321 ttcaaggtta ccaattactt tgaatttatt gcccgtgaaa cccgcgagct gatggcacag
    70381 ctgggcgtaa cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agaactggac
    70441 ggtttcaccg ccaaacagca aaaactggcg ctgtcgaagc tgctggagac cgccgaaccg
    70501 catccaggta aggcactcta ctgcaccgaa aacaacccgc cgtttgataa cggcctgctg
    70561 aacgcgcagt tgctgcaaca ggcgaaaccg tttgtcgatg agcgccagag caaaaccttc
    70621 tggttcgata ttcgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc
    70681 cagacgcacg gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc
    70741 gcaggccaga gtttcggtgt atggaacgcg ggcggcgtgg aactgtacct gaccggggat
    70801 gccaacgact atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt
    70861 ggttccgcct tccgcagcca tgaagcaagt attatcggca acacctgcct gtatggcgcg
    70921 accggtggtc gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc
    70981 ggtgctatca ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt
    71041 attgtctgta ttctgggtaa aaccggcgtt aacttcggtg cgggcatgac cggcggcttc
    71101 gcttacgttc tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actggtcgag
    71161 gtcttaagcg ttgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag
    71221 catgttcagc atactggctc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc
    71281 gccactaaat ttgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcat
    71341 cgtagtcgta gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt
    71401 cagaatgttt atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag
    71461 atccgcaaaa ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg
    71521 caggctgacc gctgcctgtc gtgcggcaac ccatactgcg agtggaaatg tccggtacac
    71581 aactacatcc cgaactggct gaagctcgcc aacgaggggc gtatttttga agcggcggaa
    71641 ctgtcgcacc agaccaacac cctgccggaa gtttgcggac gagtctgccc gcaagaccgt
    71701 ctgtgcgaag gttcctgcac tctgaacgat gagtttggcg cggtgaccat cggcaacatt
    71761 gagcgttata tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg
    71821 aagcaaaccg gtaaaaaagt ggcgattatc ggcgcaggcc cggcaggtct ggcgtgtgcg
    71881 gatgtcctga cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc
    71941 gggctgctga ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc
    72001 cgtgaaatct tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac
    72061 gtgcagctgg acgatctgct gagtgattac gatgccgtct tccttggcgt cgggacttat
    72121 cagtcaatgc gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg
    72181 ttccttatcg ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc
    72241 gtcagcatgg aaggcaaacg cgtggtggtc cttggcggtg gcgacactgc gatggactgc
    72301 gtgcgtacgt ccgtgcgcca gggagcgaag cacgttacct gtgcctatcg tcgtgatgaa
    72361 gagaacatgc cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc
    72421 aaattcaacg tccagccact ggggattgaa gtgaacggta acggtaaagt cagcggcgta
    72481 aaaatggtac gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc
    72541 gttgcaggtt ccgaacatat cgttccggca gatgcagtga tcatggcgtt tggtttccgt
    72601 ccacacaaca tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc
    72661 atcgccccgg aaggcagcga caacgccttc cagaccagca acccgaaaat ctttgctggc
    72721 ggcgatatcg tccgtggttc cgatctggtg gtaaccgcta ttgccgaagg tcgtaaggcg
    72781 gcagacggta ttatgaactg gctggaagtt taagctctgt cccagcgccg cctcctcaga
    72841 aaagagtcgg cgctgaaatt ctcctttctt tctggaaagc gcctcgcata atctgaagaa
    72901 aaccaatgta atacgctcat ctggattgtt accggtagat gcccggcgta tagctgattc
    72961 cattattcaa cgcagcgcca tataaaggga cagataatgg aatcgctctc agagagaacc
    73021 tcaacaggct accagcaaat ccacgacggt attattcacc tggtcgatag cgcccggacg
    73081 gaaacggtac gtagcgttaa cgcgctgatg accgcgacat actgggaaat tggccgacga
    73141 attgtcgaat ttgaacaagg tggcgaggcc agggctgcgt atggtgcgca gctaatcaag
    73201 cgactatcaa aggatttaag tctaaggtat aagcgtgggt tttccaccag aaatttatgg
    73261 cagtttaaga atttttatat ttgttttcaa cgaattgaaa ttgtgcagac actgtctgca
    73321 caattccccc ccacaattcg ccagacacta tctggcgaat cttgccattt agcgcaactt
    73381 gccaaatctt tccccctgcc ctggtccacc tacgtccgtt tactttccgt taaaaacgct
    73441 gacgcccgca gcttttatga aaaagagacg ctccgctgtg gctggtctgt tcgccagcta
    73501 gaacggcaaa ttgcgaccca attttatgag cgaatactac tgtcacatga caaatcagcc
    73561 atgctgcaac aacacgctcc taccgagacg catattcttc cgcaacaggc gatacgcgat
    73621 ccctttgtgc tcgaatttct ggaattgaaa gatgaatatt cagaatctga tttcgaggag
    73681 gcgctgatca accacctgat ggatttcatg ctggaacttg gggatgattt tgcctttgtt
    73741 ggtcggcagc gaaggttacg cattgatgac aactggtttc gtgtcgacct gctgtttttc
    73801 caccgccgtt tacgctgcct actaatcgtc gatctaaaag tgggcaaatt cagctatagc
    73861 gatgccggac agatgaatat gtatctcaac tacgccaaag agcactggac gctaccggat
    73921 gaaaatccgc ccatcggtct ggttctctgt gcagagaaag gagccggaga agcacattat
    73981 gctctggcag gtttgcctaa caccgttctg gcaagcgaat ataagatgca actacctgat
    74041 gagaaacgac tcgcagatga actcgttcga acacaggcgg tgctagagga aggctataga
    74101 cgccgttaat ttcagcggtt tcgaattctg cagacagtgt ctgcagaatc tttaaaacga
    74161 actaagtttt aagccattaa atcagcctta accttcacca ccaccttttt aatctcaccc
    74221 ggctcgccaa caacgcatcc cggcttatgc ggctctcctg gcataaacac ggcgaacatt
    74281 cccggtttta agatgatggt ttgctcgttt tcaatggcgc tgcaaagctg ataatcatcc
    74341 tcatggtgga actcttcaca ctgacgcgcg gtgcctgcca tgccaaacag aatccgttcc
    74401 tcaccgttta ataacagctg gatatcaatg tattgctcgt gcaattccgc ttttttctcg
    74461 acgggcgatt gagtgttaaa cgtcatgaca ttcataaaga tattgtcgcc ctgtaattcg
    74521 taacgacccg gcgctttttc ttgcggtctg gcagctaatg ccagcgttaa cgcgtcctgt
    74581 aacgcaggat gtaacccagc agacggtaat gactgtactt cacccatcat cataattttt
    74641 ctccctgggc caacagcgca gccccaagta aacctgcatc gtggcggtag tgcgccgcca
    74701 gtaaatcaac atgaaatgcc gctggctcct gcgccagata cgtttccacc agcgccagat
    74761 acccttctgc cagcccaacg ctgccaccga ccaccacgca ctggcaatca gttgtggctt
    74821 taatatcagc gatcagcctt gcaagcgtac gtgcggagcg gtgaatcagc tgctgcgcct
    74881 gctcgtcgcc ctgcccggcg cgtgtgaaaa tagttctcgc atccgcgccc gccagttcac
    74941 cctgcgctgc cgccgcaatg ccgcgaccag aagcaatcgc ctccacgcaa cctgtgcgtc
    75001 cacagccaca gaccgggccg tgtggatcgg caagcgtatg cccgatgtgc cccgccagac
    75061 cgccagggcc ggtaagcagt ttgccgccgc tcactacacc gccgccgacg ccagtggaaa
    75121 cggtgataaa gaccatatcg gttatatcgc cttccagcgc ctgatactct gcccatgctg
    75181 cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt aagttgttcc agcgttttga
    75241 ctaacggaaa gtgtagcaat ccaccgagat tatgcggatt cagcgccagc aaactgccgt
    75301 cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc atgagcttgc aacggagaga
    75361 ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt
    75421 cacgacgatc gcggatctgc ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc
    75481 cgatatcaat cgccagcgtg gtcatagcac cgcctttttc atcgctgtgt tgtaccactg
    75541 acaaatgtgt tcaagacgcg taatggcaga accgaccgtc accgcccacg cgccgtggcg
    75601 catcgcatct gccgcctgag caggcgtgtt gtaacgccct tcggcaatca ccctacatcc
    75661 ggcgtcgctc aacgttttca ccagcgccag atccggctct tctggcgttt caggcgtggt
    75721 atagccagaa agcgtagtgc caataatttc ggctcccagc ttttggcatg ccaggccgtc
    75781 ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga tggtgaatac gtgccagcag
    75841 cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc
    75901 cgcctgcgcc agcgcatcaa catcttcaat ataggccgtg atgcgtaccg gagaatcctc
    75961 cagatcgcgt ttcacaattc caataatcgg cacgctcacc accgcacgcg tggcttgcag
    76021 atttgccaca ccttcaatgc gaatggcaac cgcgcccgcc tgttctgccg ctaatgccat
    76081 ggctgcgacg atttcgggtt tatcgagcgg gctgtccgga accggctggc aggagacaat
    76141 caggccaccg ttagcagcga ttttttgatc cagttgtgca agtaacgaca tacatcttcc
    76201 cttagcgaaa ggcccggtac atagaccggg caacaggatt aacttttggt tttgactaaa
    76261 tcgtttttgg cgctgccaaa cggcacggca ccgctgaatg gtttaccgtc gatagcgtca
    76321 tgagtacgca atgcttccgg gcgcagccaa cgctgaacac gagaaggcat atccagacca
    76381 atcagcagga tcaccacaaa tgtcagactg aacgatagcg atgccagcgc agtacccaga
    76441 tccagacgtt gagcgatcaa cgcgccgatg attggggcca gtgcgccgcc caatgcgcca
    76501 acgttgtagg taaagcccag gcccgctgca cgctggtcgg tatcgaaata accgccaatc
    76561 agtttcggta agatcccggc gatcccttgt ccaagcattt gctggaagaa cagtaacaga
    76621 ccgagcaccc agacgtttgc gccgccaatc gcaaataccg gaataatcaa cagctgcgag
    76681 gccaacaggc tacaaacgta ggctttgcgg gttcccagcc agtcaccgag gaagccgccg
    76741 acacagcacc ccaccgcagc cccaaagccg ctaaagaaca gcacattggc gacagtatgc
    76801 gggttataag ccagatcggt tttcagatac gttggcagca gcgcctgaat cggccatgag
    76861 tagaggaaag caaacaacac gaccaccatc agcattacgc ccgttggcca gcgtttaccc
    76921 gtactttgca ccataaagct gataaagatt gcggcgcata acagcccaag aacagcgacg
    76981 atcgcggcat tttgcaggtt accggcgaag caaaaccaca gcgcagtagc cgccgccagt
    77041 gtcattacga tattggcaat gcgatgttca ccacggtaga gaatatctac catcgtgcgt
    77101 actggtgctt tacctgcatg tttctctttc cagtcttccg cttccgggat gtttttacgc
    77161 agccagagag caaagatgat aggcaaaatg ccgataaaga acagcgcacg ccagccccag
    77221 accggaacca ccaggctata gacctgagcg gcaacgacgg cccccacaga gaagcctgaa
    77281 atcagaaaac cactggcttt gttacgcaga tgttttggcc agctttcaat gacataggtg
    77341 gcgctggaac cgtattcacc cgccatcccc atgccgatga ccagacgcgc gataaacatg
    77401 gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg
    77461 gtgaccattg ccagacgacg cccgtagcgg tcacccatag cgccgagcat caggccgccg
    77521 aaccagcgag agataaaggc tgcggagatc agacttgccg cctgcaccgt cgtcagcccg
    77581 aattcaccct gtacttcggt gagtactagg gcgattaaaa cgaaatcaaa accgtcaagc
    77641 agatatccca accaggcagc ggaaaatgcg cgccattgtg cacggttgag atggcgatac
    77701 cacgggatat tctgggttgt agtactcatt gtgagtctcc cgcggtgggc gatgcccaca
    77761 cgctttggta taaaaattgt agggtacaga tgcgtttatt tcccctcacc cggtaggggc
    77821 gagcgagggg aaacaactca cccgcgctct tgcatcaact gctgggccag cgccttcagt
    77881 tctggcagat atttttcatc taccggtcca aacggtttgc ggcacagcgg cacagaaacg
    77941 acatccatat aatggaggac agttttcagg ccgcggaata cgcccgtttt gatcagtaaa
    78001 tcaatgactt tattgcattc agtttgcagt ttctgcgcgg tctggatatc gccttctttc
    78061 agcgccttca cgatcccctg atagcgccag cccatgatgt tgtaggtact accgatacca
    78121 ccatcagcgc ccgccagcag accagaggcg aagatttcgt cgtaaccgtt atagagcaca
    78181 agatcaggat gttcacgacg gatctgctcc atctgataga gatcgccaga ggtctgtttc
    78241 agcgcaccta cgccaggcaa tgtaacaagt gtgttgatct gatccagggt cagttttacc
    78301 ccactcaggg ctggaatgtt gtacaccacc atcggcaaac catccgccga atcaataatt
    78361 gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg
    78421 gagacggcat cgaagccata acgtttagcc gatgccgcaa gttgttggct ttcggcggtg
    78481 ctgacgcaac cgacgtgggc gatgagttta atcttacctt tcgcctcttc ggcgacgatt
    78541 tccagtacct gttcacgctc ggaaaggctt tgtacaaagg cttcgccggt cgaaccaccc
    78601 acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa ccaggcggcg cagactcgct
    78661 ttatccagtg cttgttgttg atcaaaagga gtcaggagtg cagccattac gccacgtaaa
    78721 ttcgttgcca taaatacctc tgaagtgatg cttgtctgat aaacgatata cctttatacc
    78781 tgttatacca gatcaattaa gcaacacccc atacagaaag cttataatgc gatctgcttc
    78841 actaaagtgg cattatttct ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga
    78901 gacgctgttg agatgcgatt gcaacgcacg atcggcttcg tcaggatcat gacggcggat
    78961 cgcatcaaca atcgcaatat gctgttgata actaacgttg ttatgttcgt gcagtgcctg
    79021 atcggtaacc gttgggcgtg cggcaataag ccagtcgagc agggcaacgt ggatcgccat
    79081 gaagattggg ttaccgggga tctccgccag cacgcggtgg aaatcaacgt ctgaacgaat
    79141 gaatgccgcg ttgttatcca gcgactgact gttgatttcc agtgcttttg ccagcaaatc
    79201 gatttgctca tcggtggcat gttcagccgc atagcgcacc agactggatt caaagaacag
    79261 acgtaattgt tcgaaatggg caatcccacc gggatgagaa aggaaatctt tcgccatgcc
    79321 ggaaagctca ccgatgatag tgtccgcaga aggacgcgag acgcgagcgc gttcgccgtt
    79381 gtttatttgc actagacctt tgcgttttaa cgctgccagc gcttcacgca ccgaaggacg
    79441 cccgacgtta aagaacgcca tcagttcgcg ttcagacggt aattgttcac cttcgccaaa
    79501 ttcacgacgg cggatcatct gttccagctc ttcttccacc atttcggaga gttttttacg
    79561 cgccagcggg cggctacgca agttgcgacc aattgcaggt gaagaatctt cggtttgcga
    79621 atcaaatgcg ttcataaggc ccattctgta aggtcagtgt gattaacatc atcagtgaca
    79681 tcctatcaca ggattgaaag taggggaaaa tggcagggtt ttctctt
//
LOCUS       DAJSKJ010000002        67268 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000002 DAJSKJ010000000
VERSION     DAJSKJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.002"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(60..917)
                     /gene="murI"
                     /locus_tag="ON345_000081"
     CDS             complement(60..917)
                     /gene="murI"
                     /locus_tag="ON345_000081"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR3070204.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(862..2706)
                     /gene="btuB"
                     /locus_tag="ON345_000082"
     CDS             complement(862..2706)
                     /gene="btuB"
                     /locus_tag="ON345_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR3070205.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2771..2961)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3075..4175
                     /gene="trmA"
                     /locus_tag="ON345_000083"
     CDS             3075..4175
                     /gene="trmA"
                     /locus_tag="ON345_000083"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR3070206.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4215..4574)
                     /locus_tag="ON345_000084"
     CDS             complement(4215..4574)
                     /locus_tag="ON345_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR3070207.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4574..5224)
                     /gene="fabR"
                     /locus_tag="ON345_000085"
     CDS             complement(4574..5224)
                     /gene="fabR"
                     /locus_tag="ON345_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR3070208.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5555..6955
                     /gene="sthA"
                     /locus_tag="ON345_000086"
     CDS             5555..6955
                     /gene="sthA"
                     /locus_tag="ON345_000086"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR3070209.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6938..7855)
                     /gene="oxyR"
                     /locus_tag="ON345_000087"
     CDS             complement(6938..7855)
                     /gene="oxyR"
                     /locus_tag="ON345_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR3070210.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8122..9495)
                     /gene="argH"
                     /locus_tag="ON345_000088"
     CDS             complement(8122..9495)
                     /gene="argH"
                     /locus_tag="ON345_000088"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR3070211.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9556..10332)
                     /gene="argB"
                     /locus_tag="ON345_000089"
     CDS             complement(9556..10332)
                     /gene="argB"
                     /locus_tag="ON345_000089"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR3070212.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10340..11344)
                     /gene="argC"
                     /locus_tag="ON345_000090"
     CDS             complement(10340..11344)
                     /gene="argC"
                     /locus_tag="ON345_000090"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR3070213.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11498..12649
                     /gene="argE"
                     /locus_tag="ON345_000091"
     CDS             11498..12649
                     /gene="argE"
                     /locus_tag="ON345_000091"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR3070214.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVELQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            13001..15652
                     /gene="ppc"
                     /locus_tag="ON345_000092"
     CDS             13001..15652
                     /gene="ppc"
                     /locus_tag="ON345_000092"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR3070215.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15835..17568
                     /locus_tag="ON345_000093"
     CDS             15835..17568
                     /locus_tag="ON345_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR3070216.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPVAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17783..18634
                     /locus_tag="ON345_000094"
     CDS             17783..18634
                     /locus_tag="ON345_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3070217.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRNLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18621..18962)
                     /locus_tag="ON345_000095"
     CDS             complement(18621..18962)
                     /locus_tag="ON345_000095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR3070218.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18964..19842)
                     /locus_tag="ON345_000096"
     CDS             complement(18964..19842)
                     /locus_tag="ON345_000096"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR3070219.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(19808..22105)
                     /locus_tag="ON345_000097"
     CDS             complement(19808..22105)
                     /locus_tag="ON345_000097"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3070220.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22156..22476)
                     /locus_tag="ON345_000098"
     CDS             complement(22156..22476)
                     /locus_tag="ON345_000098"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR3070221.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22491..23570)
                     /locus_tag="ON345_000099"
     CDS             complement(22491..23570)
                     /locus_tag="ON345_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR3070222.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23879..26380
                     /gene="ptsP"
                     /locus_tag="ON345_000100"
     CDS             23879..26380
                     /gene="ptsP"
                     /locus_tag="ON345_000100"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3070223.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSVLALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26392..27054
                     /gene="fsa"
                     /locus_tag="ON345_000101"
     CDS             26392..27054
                     /gene="fsa"
                     /locus_tag="ON345_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR3070224.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27065..28168
                     /gene="gldA"
                     /locus_tag="ON345_000102"
     CDS             27065..28168
                     /gene="gldA"
                     /locus_tag="ON345_000102"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR3070225.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28442..29059
                     /locus_tag="ON345_000103"
     CDS             28442..29059
                     /locus_tag="ON345_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR3070226.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29086..29991)
                     /gene="yijE"
                     /locus_tag="ON345_000104"
     CDS             complement(29086..29991)
                     /gene="yijE"
                     /locus_tag="ON345_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR3070227.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLVIALLQTCGMVGLVQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(30085..32265)
                     /gene="katG"
                     /locus_tag="ON345_000105"
     CDS             complement(30085..32265)
                     /gene="katG"
                     /locus_tag="ON345_000105"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR3070228.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32594..33484)
                     /gene="metF"
                     /locus_tag="ON345_000106"
     CDS             complement(32594..33484)
                     /gene="metF"
                     /locus_tag="ON345_000106"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR3070229.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33833..36265)
                     /gene="metL"
                     /locus_tag="ON345_000107"
     CDS             complement(33833..36265)
                     /gene="metL"
                     /locus_tag="ON345_000107"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR3070230.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNASETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36268..37428)
                     /gene="metB"
                     /locus_tag="ON345_000108"
     CDS             complement(36268..37428)
                     /gene="metB"
                     /locus_tag="ON345_000108"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR3070231.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37705..38022
                     /gene="metJ"
                     /locus_tag="ON345_000109"
     CDS             37705..38022
                     /gene="metJ"
                     /locus_tag="ON345_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR3070232.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38206..38814
                     /locus_tag="ON345_000110"
     CDS             38206..38814
                     /locus_tag="ON345_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR3070233.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(38875..39087)
                     /gene="rpmE"
                     /locus_tag="ON345_000111"
     CDS             complement(38875..39087)
                     /gene="rpmE"
                     /locus_tag="ON345_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR3070234.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            39290..41488
                     /gene="priA"
                     /locus_tag="ON345_000112"
     CDS             39290..41488
                     /gene="priA"
                     /locus_tag="ON345_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR3070235.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            41644..42669
                     /gene="cytR"
                     /locus_tag="ON345_000113"
     CDS             41644..42669
                     /gene="cytR"
                     /locus_tag="ON345_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR3070236.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAQEVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKCQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            42761..43720
                     /gene="ftsN"
                     /locus_tag="ON345_000114"
     CDS             42761..43720
                     /gene="ftsN"
                     /locus_tag="ON345_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR3070237.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            43813..44343
                     /gene="hslV"
                     /locus_tag="ON345_000115"
     CDS             43813..44343
                     /gene="hslV"
                     /locus_tag="ON345_000115"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR3070238.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            44353..45684
                     /gene="hslU"
                     /locus_tag="ON345_000116"
     CDS             44353..45684
                     /gene="hslU"
                     /locus_tag="ON345_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR3070239.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            45751..46677
                     /gene="menA"
                     /locus_tag="ON345_000117"
     CDS             45751..46677
                     /gene="menA"
                     /locus_tag="ON345_000117"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR3070240.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            46770..47255
                     /gene="rraA"
                     /locus_tag="ON345_000118"
     CDS             46770..47255
                     /gene="rraA"
                     /locus_tag="ON345_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR3070241.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(47340..47585)
                     /gene="zapB"
                     /locus_tag="ON345_000119"
     CDS             complement(47340..47585)
                     /gene="zapB"
                     /locus_tag="ON345_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR3070242.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            48011..48856
                     /gene="glpF"
                     /locus_tag="ON345_000120"
     CDS             48011..48856
                     /gene="glpF"
                     /locus_tag="ON345_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR3070243.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESIDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            48879..50387
                     /gene="glpK"
                     /locus_tag="ON345_000121"
     CDS             48879..50387
                     /gene="glpK"
                     /locus_tag="ON345_000121"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR3070244.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            50523..51533
                     /gene="glpX"
                     /locus_tag="ON345_000122"
     CDS             50523..51533
                     /gene="glpX"
                     /locus_tag="ON345_000122"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR3070245.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            51630..52376
                     /gene="fpr"
                     /locus_tag="ON345_000123"
     CDS             51630..52376
                     /gene="fpr"
                     /locus_tag="ON345_000123"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR3070246.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETREMTKHLRRRPGHMTAEHYW"
     gene            complement(52381..52809)
                     /gene="uspD"
                     /locus_tag="ON345_000124"
     CDS             complement(52381..52809)
                     /gene="uspD"
                     /locus_tag="ON345_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR3070247.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPVYRGMINKLSADLLIVPFIDK"
     gene            complement(52836..53135)
                     /locus_tag="ON345_000125"
     CDS             complement(52836..53135)
                     /locus_tag="ON345_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR3070248.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(53347..53787)
                     /locus_tag="ON345_000126"
     CDS             complement(53347..53787)
                     /locus_tag="ON345_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR3070249.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMVVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAVVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            53888..54487
                     /locus_tag="ON345_000127"
     CDS             53888..54487
                     /locus_tag="ON345_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR3070250.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            54595..55362
                     /gene="tpiA"
                     /locus_tag="ON345_000128"
     CDS             54595..55362
                     /gene="tpiA"
                     /locus_tag="ON345_000128"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3070251.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(55417..56172)
                     /gene="cdh"
                     /locus_tag="ON345_000129"
     CDS             complement(55417..56172)
                     /gene="cdh"
                     /locus_tag="ON345_000129"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR3070252.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLIPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(56279..57268)
                     /gene="sbp"
                     /locus_tag="ON345_000130"
     CDS             complement(56279..57268)
                     /gene="sbp"
                     /locus_tag="ON345_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR3070253.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(57588..58550)
                     /gene="pfkA"
                     /locus_tag="ON345_000131"
     CDS             complement(57588..58550)
                     /gene="pfkA"
                     /locus_tag="ON345_000131"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR3070254.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(58731..59633)
                     /gene="fieF"
                     /locus_tag="ON345_000132"
     CDS             complement(58731..59633)
                     /gene="fieF"
                     /locus_tag="ON345_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR3070255.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(59782..60282)
                     /gene="cpxP"
                     /locus_tag="ON345_000133"
     CDS             complement(59782..60282)
                     /gene="cpxP"
                     /locus_tag="ON345_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR3070256.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTSENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            60432..61130
                     /gene="cpxR"
                     /locus_tag="ON345_000134"
     CDS             60432..61130
                     /gene="cpxR"
                     /locus_tag="ON345_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR3070257.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            61127..62500
                     /gene="cpxA"
                     /locus_tag="ON345_000135"
     CDS             61127..62500
                     /gene="cpxA"
                     /locus_tag="ON345_000135"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR3070258.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(62606..63280)
                     /gene="yiiM"
                     /locus_tag="ON345_000136"
     CDS             complement(62606..63280)
                     /gene="yiiM"
                     /locus_tag="ON345_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR3070259.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(63429..64412)
                     /gene="kdgT"
                     /locus_tag="ON345_000137"
     CDS             complement(63429..64412)
                     /gene="kdgT"
                     /locus_tag="ON345_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR3070260.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(64672..65292)
                     /gene="sodA"
                     /locus_tag="ON345_000138"
     CDS             complement(64672..65292)
                     /gene="sodA"
                     /locus_tag="ON345_000138"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR3070261.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            65577..66611
                     /gene="rhaT"
                     /locus_tag="ON345_000139"
     CDS             65577..66611
                     /gene="rhaT"
                     /locus_tag="ON345_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR3070262.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(66608..>67268)
                     /gene="rhaR"
                     /locus_tag="ON345_000140"
     CDS             complement(66608..>67268)
                     /gene="rhaR"
                     /locus_tag="ON345_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR3070263.1"
                     /translation="DLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGF
                     SASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYT
                     SDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQ
                     YLRQVRVCHAQYLLQHSRRLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
BASE COUNT        16465 a        18128 c        17126 g        15549 t
ORIGIN      
        1 ttccggcctg acaagaggaa atttaaaata attttctgac cgcgcaacat tcaaccaaat
       61 cagcctaaaa ctgccagttt ttcgagcgtt tcgaagccgt aacgctgtaa aacgggcaat
      121 aattgttcag cttctggcgt cattgccata caaaaggcaa tattcgcatc ggcagatttt
      181 gcatccggag cttcatgttc taacagccag gccgttctgc gagcaatcgc tgcgccagaa
      241 tccaccagcc gggttccctc tggcagcacc tgtaagagtt cttcttgtag tagagggaaa
      301 tgggtgcaac ccaatacaac ggtatctggc ggctctttca ttcttaacca cgggcgtagg
      361 atacgtttta gtgcatccag agaaacatct tcgccatgca gcttcgcttc agccaactca
      421 accatctctg ccgagcccag catttctatc tggcattcat tagcgaaacg cgcgatcagc
      481 tcatgagtat aagaacgttt aacagttccg cgggttgcca gtaatccgac aatgccattt
      541 gccgtcagac gtgcagcagg tttaatcgcc ggcacgacac caacaaccgg gaagtcgaac
      601 ttttcgcgta atgcaggaag tgaaacggta ctggcggtgt tgcaagcgac cacagccagc
      661 gcaaggggat aacgttcttg cactgcggtg acaattgcca ccactcgctc aacaataaac
      721 tcttcgcttt tttcgccata cggaaaagcg acgttatcga aagcataaat gtaatggaga
      781 tccggtaaga gatgccggat ctcgtcatag accgacaacc caccgacgcc ggagtcaaac
      841 accagcacgg tgggacgtgg ttcagaaggt gtagctgcca gacaaggtgt attcccgtcc
      901 tgcagtttgg tagccataga ctgtctcata atctttgtcg aacaggttgg ctattttacc
      961 acgaactgtc aggtgagagg tgaccggata cgcaaccgca agatcccaca agctcacacc
     1021 gcccatttta acggtttgat aaggataaga tgagtaatcc ttatcatagc gagtgcctaa
     1081 atactgataa gtaatacccc agtcgaagtc atacaactgc cagtcgagct ggtatttcac
     1141 ctgctgttta gcacggcgta acaacggcgt gtcggtaatc gcattgcgcg catcgacata
     1201 atcataactc acagtatgcg tcagtggtcc ggtatcaaaa ttggcggtcg cctcgacgcc
     1261 cttaatccgc gctttccctt cgttgtaata tttcagggtg tgatcatcat aatcgatcaa
     1321 gtcactgaca tcgttacgat atccggaaat acgccagttc accccagcgg ttaagccttc
     1381 aaacgcgcct tcccactgtt tgcttttctc cgggtccaga ttcggatttc cgtagaagcc
     1441 atacagttgc cccagatttg gtgccttata agatgtcccg taggaagcaa tgaagcgata
     1501 accttcgatg aattcccaac cggcgctggt ttgccaggtt ccatgacgac caaactgtga
     1561 gttatcgtca ctgcgtgctg cgccttcaaa ggtaaaatcg ccgacttgtt gcagcccggt
     1621 cagatagatg ccggtattac gttgatcata tccattctca acataacctg tacccggcgt
     1681 cgtagtctgt ttctgccagt cgacacccgc accaatacta ccgtgaccaa cgatgacatt
     1741 gtttgcccac tggacggtgt attgcttcat ctcatcgagc gtcgccgacg aatcataacg
     1801 accataatgg ggatcgtagt tgtaatcttt gctatggcta tagctggtaa tgagttgtga
     1861 tttaatcagt tcgccgttat agcgcagccc ggcgtcccaa ctttggctat agagtttacg
     1921 ggtatcgagc aacggtgaac cgggagaata atacgcgtca taattggtac ggttatcata
     1981 gccatagccg cgcacaaagc cgctccaggc atcagtaaag ttatgctcca gcgcgccata
     2041 aagcgtttta cttaaaaaac catcgttatc tgtctgcgct tgcgttccgg tattaccata
     2101 ggcaacaaca tcataaccat gagtatgggc ataatcgccc aacagcgtta cccgtgtctt
     2161 atcccccagt tgttgctgcg tagagacatc atagttctga taactattgc ttccccaccc
     2221 tgctgaaatt tccgttccgg gttcatcgcg cgtcgtgatg atattcacca ccccgcctat
     2281 tgcatcggaa ccataaacag cggagcgcgg cccacggata tattcaacac gctggacaag
     2341 cgcaataggg aactggctaa ggtcggcaga accactcacc cccgccagat tcaggcgtac
     2401 gccatcaatt aacaccaaca catgactggc atttgtaccg cgaataaaaa tagatgagag
     2461 ctgacctgaa ccgccgtttt gggtgatatc gacgcccgga agacggcgca gcacatcatt
     2521 gaccgaggtc gactgccagc ggtcgatatc ctgacgggtc acaacggtgg ttggtgcaag
     2581 cacagtgctg cgcggctgtt caaaacggtt agcagtaacg acgagagtat ccgggctggt
     2641 atcctgtgcc caagcggaaa aggctgtgac agaacacgcc gtcagcagcg aagctttttt
     2701 aatcattgta aagcatccac aatagaagaa ggatgccgca ggtttcatca atattacgcg
     2761 atgatgagaa ccagatgcga cgttggccgg caggtcttcg ggcttggagg ggtatctaag
     2821 atactaagag atgatgactt cccaccggat ggcagtgtcc gcataacgca atcatcgcac
     2881 ctttccttac cgctgcgcgt cagctccaga ttcgcactgg attccctatt aactcacagg
     2941 accggcaagt ggatgctaca ggttgtaaca agttactgtc cagacgtagc tcacaaatag
     3001 gaattcatca agatctggac atctgatgag caatccctac aatcgccgcg tactttaatt
     3061 tttcaggata catcatgacc cccgaacacc ttccaacaga acagtatgaa gcgcagttag
     3121 ccgaaaaagt ggtacgtttg caaagtatga tggcaccgtt ttctgacctg gttccggaag
     3181 tgtttcgctc gccggtcagt cattaccgga tgcgtgcgga gttccgcatc tggcacgatg
     3241 gcgatgacct gtatcacatc attttcgatc aacaaaccaa aagccgtatc cgcgtggata
     3301 gcttccccgc cgccagtgaa cttatcaacc agttgatgac ggcgatgatt gcgggtgtgc
     3361 gtaataatcc cgttctgcgc cacaagttgt tccagattga ttacctcacc acgctgagta
     3421 atcaggcggt ggtttccctg ctataccata agaagctgga tgatgagtgg cgtcaggaag
     3481 cggaggccct gcgcgatgca ctgcgcgcgc agaatctgaa tgtgcatctg attggtcggg
     3541 caacgaaaac caaaatcgag ctggatcaag attacatcga cgagcgtctg ccggtcgcag
     3601 ggaaagagat gatctaccgt caggtagaaa acagctttac ccagccgaac gcggcgatga
     3661 atattcagat gctggaatgg gcgctggacg taaccaaagg ctcaaaaggc gatttactgg
     3721 agctgtactg cggcaacggt aacttttcat tagcgctggc gcgcaatttt gatcgggtat
     3781 tagccaccga aatcgctaag ccgtcggttg ctgctgcgca atacaacatc gcagctaacc
     3841 atattgataa cgtacaaatt attcgtatgg cggcagaaga atttactcag gcgatgaatg
     3901 gtgtgcgcga gtttaaccgc ctgcaaggga tcgacttaaa gagttatcag tgcgaaacca
     3961 tttttgtcga cccaccgcgc agcggtctgg acagtgaaac cgagaaaatg gtgcaggcgt
     4021 atccgcgtat tttgtacatc tcctgtaacc cggaaacgtt atgcaagaat ctggaaacat
     4081 taagccagac gcacaaggtc gaacgtctgg ctctgtttga tcagttcccc tacacgcacc
     4141 atatggagtg cggcgtatta ctgaccgcga agtaaaaccc atgccggatg cgccagcatc
     4201 cggcataata ccgattactc agcaacttcc tgcttacggt tacgcatctt cgcgccaatc
     4261 cagaacacca taatgacgga gagtactgcc gggaagaagt tagagccgat atccggatat
     4321 tccgcacgaa ctaccgtgct gtacagcagt acgccgagaa taaaacaggc agctgccaga
     4381 cccggcaagc ctaccggcat agtgcgatta agataacgtt gatgcaggca gtaaaccgtc
     4441 agcaccaggg aaataatcgg gaatacagaa aatggcacaa tggagctaaa cagcgctgcg
     4501 aaagtaccat taatcgataa gccagcaact aacgccagca gcagcgtacc tctatcttga
     4561 tttgcttgtt tcattactcg tccttcacat ttcccggaat aattgcggtt ttctcttgtt
     4621 cacggcgata ccagtaataa gcccctttcg aaatcattcg cagttgcagt accagtcgct
     4681 cttctaattg ccgacgttgt tcgacgccga cgtccaacgc ctcggcaccc gcactgaaga
     4741 caattgtcac cattgcttcg gcttgcgctt cagtaaacgc acgcggcata tggttttcga
     4801 gttccagata gtccgcaagt tccgcaatga agtgctgaat ttcacgcgca acggcggcac
     4861 gaaacgcagc ggaggtgccg gagcgttccc gcaataataa ccggaaggcg ttaggattat
     4921 taccgatgaa ctccataaat gtggagaccg aggtgcggat cacactcccg cctttggcga
     4981 tacgctgacg cgcctggcgc atgagttggc gtagcattaa accgctctca tcaaccatgg
     5041 tcagacccag ttcgtctacg tcgcggaaat gccgataaaa agaggtggga gcaatgcccg
     5101 cttcacgcgc cacttcacgc aaactcaggc tggcgaagct gcgttcagca cttaattggc
     5161 taaatgcggc ttccaccagc gaacggcggg ttttttcttt ttgttgcgct cttacgccca
     5221 tcacgatgtc tgaatccttg ccaaaagtac tgctggcact ataccagaga atgaacatac
     5281 tggattgctc cagtattgtg aatgaacggt aacgcaataa aacatattta ctgcaattct
     5341 ttggccggtt cttttacgta cagcggaaac ctgccgctta aacggagagt atcgtcgata
     5401 aaaatccaat aaaacgtcag ggcaaaagta agaaacagac aaagcaaagg ccgctcagga
     5461 tatagccaga taaatgacgg ggatcaattg gcttacccgc gataaaatgt taccattctg
     5521 ttgcttttat gtataagaac aggtaagccc taccatgcca cattcctacg attacgatgc
     5581 catagtaata ggttccggcc ccggcggcga aggcgctgca atgggcctgg ttaagcaagg
     5641 tgcgcgcgtc gcagttatcg agcgttatca aaatgttggc ggcggttgca cccactgggg
     5701 caccatcccg tcgaaagctc tccgtcacgc cgtcagccgc attatagaat tcaatcaaaa
     5761 cccactttac agcgaccatt cccgactgct ccgctcttct tttgccgata tccttaacca
     5821 tgccgataac gtgattaatc aacaaacgcg catgcgtcag ggattttacg aacgtaatca
     5881 ctgtgaaata ttgcagggaa acgctcgctt tgttgacgag catacgttgg cgctggattg
     5941 cccggacggc agcgttgaaa cactaaccgc tgaaaaattt gttattgcct gcggctctcg
     6001 tccatatcat ccaacagatg ttgatttcac ccatccacgc atttacgaca gcgactcaat
     6061 tcttagcatg caccacgaac cgcgccatgt acttatctat ggtgctggag tgatcggctg
     6121 tgaatatgcg tcgatcttcc gcggtatgga tgtaaaagtg gatctgatca acacccgcga
     6181 tcgcctgctg gcatttctcg atcaagagat gtcagattct ctctcctatc acttctggaa
     6241 cagtggcgta gtgattcgtc acaacgaaga gtacgagaag atcgaaggct gtgacgatgg
     6301 tgtgatcatg catttgaagt cgggtaaaaa actgaaagct gactgcctgc tctatgccaa
     6361 cggtcgcacc ggtaataccg attcgctggc gttacagaac attgggctgg aaactgacag
     6421 tcgcggacag ctgaaggtca acagcatgta tcagaccgca cagccgcacg tttacgcggt
     6481 gggcgacgtg attggttatc cgagcctggc atcagcggcc tatgaccagg ggcgcattgc
     6541 cgcgcaggcg ctggtaaaag gcgaagccac cgcgcatctg attgaagata tccctaccgg
     6601 catttacacc atcccggaaa tcagctctgt gggcaaaacc gaacagcagc tgaccgcgat
     6661 gaaagtgcca tatgaagtgg gccgcgccca gtttaaacat ctggcacgcg cacaaatcgt
     6721 cggcatgaac gtgggcacgc tgaaaatttt gttccatcgg gaaacaaaag agattctggg
     6781 tattcactgc tttggcgagc gcgctgccga aattattcat atcggtcagg cgattatgga
     6841 acagaaaggt ggcggcaaca ctattgagta cttcgtcaac accaccttta actacccgac
     6901 catggcggaa gcctatcggg tagctgcgtt aaacggttta aaccgcctgt tttaacactt
     6961 tatcgaaatg gccatccatt cttgctcgga tggcctctgc cagctgctca tagcggctgc
     7021 gcagcggtga gccaggacga taaaccaggc caatagtgcg gcgtggttcc ggcttaatgc
     7081 acggcagata aacaacccca tcgcgtttgc gctccggcgg cacagccagc gctggcagta
     7141 aagtgatccc gctacctgcc gccaccatgt tgcgcagcgt ctccaggctg gtcgcgcgga
     7201 agtgtgtatc ttcatccgcc ccggcttcaa aacagaaacc cattgcctga tcgcgcaaac
     7261 agtgaccatc ttccagcatc agcagttttt cccctgccag atcggccatc ggtacgcatt
     7321 cgcggttcgc ccacgggtga tcttcataga tagccagcaa cattggctca tcaaacaacg
     7381 gcacttcaat gaatgcttcg ctctctttca ccagcgcgag gatcacgcaa tcgagtttgc
     7441 cgctgtccag ttgcgccagt aactggtggg tctgtgcttc atgcagatac atttccagct
     7501 ttggaaaggt ctggtgcagc atagggataa tatgcggtag caggtacggt ccaactgtgg
     7561 gaatcaaacc aatgtgcagc ggcccggaca tcgtctcgcc ctgctggctt gccatctctt
     7621 taaggacttt cacctcacgc agtacggtac gcgcctgatc taccagcagc attcccgcct
     7681 gggtgaacaa cactttacgg ctggtccgct ccagcaacat cacgcccaac tcatcttcca
     7741 gcttacgaat ttgcccgcta agcgtcggct ggctaacgtg gcaggaatct gccgcacgcc
     7801 gaaaatggcg gtgttcggcc aatgccacca ggtactcaag atcacgaata ttcattatcc
     7861 atcctccatt gccacgatag ttcatggcga taggtagaat agcaatgaac gattatccct
     7921 atcaagcatt ctgactgata attgctcaca gaaacggagc ggcacctctt ttaacccttg
     7981 aagtcactgc ccgtttcgag agtttctcaa ctcgaataac taaagccaac gtgaactttt
     8041 gcggatctcc aggatccgct tttttttgcc ataaaaaagc ccggcgataa gccaggctca
     8101 aatttataca tataaatgtt cttaccccaa ccgcgcctgc gcaaaagcaa ttgcctgcgc
     8161 cacctgctgc ggtgagacgc cgccttttgc cgcacgcttg tcgaggcacg attgcagcga
     8221 cagaatcgga tagacatctt cgtcaatcac cgggctgaat ttctgcaact cgtcgagcgg
     8281 cagatcttcc agcggtttgc cctgacgaat ggcttccacc accgcttcac caacaatatg
     8341 gtgcgcctcg cggaacggta cgcctttcgc caccagataa tccgccagtt cggtggcgtt
     8401 ggcgtaaccc tgctgagccg cttcctggca acgtggacgt ttcacctgaa tgccgtccag
     8461 caccagcgcc gccatatgca ggcagtccag ccaggtatcg agcgcgtcga acagaccttc
     8521 tttgtcttcc tgcatatctt tgttgtaagc cagcggcaaa cctttcagcg tcatcatcat
     8581 gccggttaac gccccctgca cccgaccgca tttaccgcga atcagctcca gcgcatccgg
     8641 gtttttcttc tgcggcatta atgatgaacc ggaagtcacg cggtcagaaa gctccacaaa
     8701 ccccgcttcg ccggtgttaa agaaaatcag atcttcagca aaacgcgaca gatgcaccat
     8761 gccgatagcg gcagcagaaa gcagttccaa cacatggtca cggtcagaaa cgctgtcgag
     8821 actgttacgg gtcgccgaag caaagcccag ccagcctgct aactgttcac ggtcgatttc
     8881 ataggccgtt cccgccagcg cgccacagcc tagcgggctg acatccagac gcttaagcgc
     8941 atcctgcaaa cggctttcat cacgcgccag catctcaaca taggccaggc accagtgcgc
     9001 gaacgtcacc ggctgggcgc gttgcaggtg agtgtaacct ggcattaccg cgtcctgatt
     9061 gttttgtgcg gtttccacca gcgccgattg cagctgccgg ttagccgtca gtaactcgct
     9121 aacggtatct ttgcaccaca gtttcaggtc agtcgctacc tgatcattac ggctacgccc
     9181 ggtatgcagc tttttgccta actggcccac tttgtcgatc agtttgcctt ccacccagct
     9241 atggatatct tcggcgtcgc tttcaaggat ttgttgtggc ctggcgcgaa catcttccag
     9301 caacacgttc agcgcctctt ccagttgcgc ctgctcttct gcggttaaca cgcctaccgt
     9361 gactagggct ttggaccagg ccacagagcc aacaatatcc tgctccgcca gacggtaatc
     9421 aaagcgcagt gagtcgttga attgtttgaa ccgttgatct gctgcctggg taaaacgccc
     9481 gccccaaagt gccataactc tgtttcctta ttttgaaatt caatgccgga aagctgcgcc
     9541 tccggccaac aaaacttaag ctaaaatccg cgtacccatc ggcataccgt taaacagtgc
     9601 cggaagctgc tccgcatgac gccaggaggc gatatctacc ggacggccca gagtgcgggc
     9661 cgcatccagc gccgcgttca ctttcactat catgccgtca gtaataatgc cctgctcaat
     9721 cagttgttct gctttcgcgg cggtcatttc ggcaatgcgt tgccctttgc cgtcgagaat
     9781 gccgctgacg tcggagagca aaatcagatc cgcgcccagc gttgccgcca gcgccgttgc
     9841 cgcctggtcg gcattgacgt tcatcagttg cccttcgtct gttacgccaa tggagctgac
     9901 caccggcaga taaccgttct ccagcaagga gttgataagc ttaggcgaac ctggctgcgc
     9961 cagtccaaca tgacctaact cttcatcaag ctgggtcact ttgacgctgt cgccgtcacc
    10021 gagaaacaaa cctacggccg caatctgatg tttcttcgcc catgccaaca gggttttatt
    10081 tgccgttccc gccagtgctc cggtgataat gtctatctga tcagcaggcg tcacccgcag
    10141 gccgtttttc tttttcaccg gcagattcag ccctttcatc agctcatcca ccacgcaacc
    10201 gccgccgtgc acaatcacca gcggacgctg atgtgactca cgataattca ccagtgcgct
    10261 aaacagacgt tccagcgcct cttcactatc cagcagtacg ccgcccagtt tgataattaa
    10321 tggattcatc attgcaccct taaataagag actgcgtttc cgcatagccg aaacgaatat
    10381 tggcgcactg taccgcttgt gccgccgcgc ctttcagtaa gttgtcttcg gtcgccacaa
    10441 taatcaaatg ctcgccctga acggcaaacc cgatatcgca aaatggcagc ccaacgacat
    10501 ttttcagcgc cggaacgcct ttgtcataca gccgcaccag cggtttatgg gcatacgcct
    10561 gctgtaacac ttgcgcgact tgcgcctggg tcacacccga tttcaggcgg caggtaatgg
    10621 tttcgagaat gccgcgcggg aaattgccca gatgtggggt gaagataacg tcagcaccga
    10681 ggtgtgtggc gatctctggt tgatggcgat gagtaaagac gccatacggt tgcaggctaa
    10741 cttcacaaaa actatttgaa atcgctgcct tacgtcctgc accgctcacg ccgctggtgg
    10801 cgttgatcac cggccactga ttgaggtcaa gaagatcggc atcaatcaac ggtttcagcg
    10861 ccagctgtgc cgccgtcgga taacagcctg gcaccgcaat caaattcgct tcttttaatt
    10921 tattaccgca ccactccgcc agaccgtagg ctgcctgttc caacagttcc gggtattgat
    10981 gggtaaagcc gtaatatttt tcatagaagg tggcgtcgtt aacacgaaac gcgccggaaa
    11041 ggtcgaacac cacgcagccc gcttcaagaa actgcggcgc taaatcgtgg ctaacttcat
    11101 gggcagtggc gagaaacacc acgtccaccc ctgggctaaa ctcgctgata tccgacatcg
    11161 gctgcaacgg cagctcaacg atgcctttta gctgcggatg caaatcggag attaactttc
    11221 ccgcatcatt gctttgcgct gaaacagtca aagcggttat gttcatatgc ggatggcgat
    11281 ttacataggt cactagctct gcgccagcgt agccgctggc acccacaatc agcgtattca
    11341 acatcggggc tattcacctt cttatgtctg gttgccaggt taaacgtaaa acattcacct
    11401 tacggctggt gggttttatt acgctcaacg ttagtgtatt tttattcata aatactgcat
    11461 gaatattgat actatcatga ccagaggtgt gtcaacaatg aaaaacaaat taccgccatt
    11521 tatcgagatt taccgtgctc tgattgccac accttcaata agcgccacgg aagaggcact
    11581 cgatcaaagc aatgcagatt taatcactct gctggcggac tggtttaaag atttaggctt
    11641 caatgtggaa ctgcagcctg tgccaggaac tcgcaacaaa ttcaatatgc tggcaagtat
    11701 cggacagggg gctggcggct tgttgctggc ggggcatacc gatacggtgc catttgatga
    11761 cggtcgctgg acgcgcgatc cgtttacact gacggagcat gacggcaagc tttacggctt
    11821 aggcaccgcc gacatgaaag gcttttttgc gtttatcctt gatgcgctac gcgatgtcga
    11881 cgtcacgaaa ctgaaaaaac cgctctacat tctggcgact gctgatgaag aaaccagtat
    11941 ggccggagcg cgttattttg ccgaaactac cgccctgcgc ccggattgcg ccatcattgg
    12001 cgaaccgacg tcactacaac cggtacgcgc acataaaggt catatctcta acgccatccg
    12061 tattcagggc cagtcggggc actccagcga tccagcacgc ggagttaacg ctatcgaact
    12121 aatgcacgac gccatcgggc atattttgca attgcgcgat aacctgaaag aacgttatca
    12181 ctacgaagcg tttaccgtgc cataccctac gctcaacctc gggcatattc acggtggcga
    12241 cgcttctaac cgtatttgcg cttgctgtga gttgcatatg gatattcgtc cgctgcctgg
    12301 catgacactc aatgaactta atggtttgct caacgatgca ttggctccgg tgagcgaacg
    12361 ctggccgggt cgtctgacgg tcgacgagct gcatccgccg atccctggct atgaatgccc
    12421 accgaatcat caactggttg aagtagttga gaaattgctc ggagcaaaga ccgaagtggt
    12481 gaactactgt accgaagcgc cgtttattca aacgttatgc ccgacgctgg tgttggggcc
    12541 tggctcaatt aatcaggctc atcaacctga tgaatatctg gaaacacggt ttatcaagcc
    12601 cacccgcgaa ctgataaccc aggtaattca ccatttttgc tggcattaaa acgtaggccg
    12661 gataaggcgc tcgcgccgca tccggcactg ttgtcaaact ccagtgccgc aataatgtcg
    12721 gatgcgatac ttgcgcatct tatccgacct acacctttgg tgttacttgg ggcgattttt
    12781 taacatttcc ataagttacg cttatttaaa gcgtcgtgaa tttaatgacg taaattcctg
    12841 ctatttattc gtttgctgaa gcgatttcgc agcatttgac gtcaccgctt ttacgtggct
    12901 ttataaaaga cgacgaaaag caaagcccga gcatattcgc gccaatgcga cgtgaaggat
    12961 acagggctat caaacgataa gatggggtgt ctggggtaat atgaacgaac aatattccgc
    13021 attgcgtagt aatgtcagta tgctcggcaa agtgctggga gaaaccatca aggatgcgtt
    13081 gggagaacac attcttgaac gcgtagaaac tatccgtaag ttgtcgaaat cttcacgcgc
    13141 tggcaatgat gctaaccgcc aggagttact caccacctta caaaatttgt cgaacgacga
    13201 gctgctgccc gttgcgcgtg cgtttagtca gttcctgaac ctggccaaca ccgccgagca
    13261 ataccacagc atttcgccga aaggcgaagc tgccagcaac ccggaagtga tcgcccgcac
    13321 cctgcgtaaa ctgaaaaacc agccggaact gagcgaagac accatcaaaa aagcagtgga
    13381 atcgctgtcg ctggaactgg tcctcacggc tcaccctacc gaaattaccc gtcgtacact
    13441 gatccacaaa atggtggaag tgaacgcctg cttaaaacag ctcgataaca aagatatcgc
    13501 tgactacgaa cacaaccagc tgatgcgtcg cctgcgccag ttaatcgccc agtcatggca
    13561 taccgatgaa atccgtaagc tgcgtccaag cccggtagat gaagccaaat ggggctttgc
    13621 cgtagtggaa aacagcctgt ggcaaggcgt accaaattac ctgcgcgaac tgaacgaaca
    13681 actggaagag aacctcggct acaaactgcc cgtcgaattt gttccggtcc gttttacttc
    13741 gtggatgggc ggcgaccgcg acggcaaccc gaacgtcact gccgatatca cccgccacgt
    13801 cctgctactc agccgctgga aagccaccga tttgttcctg aaagatattc aggtgctggt
    13861 ttctgaactg tcgatggttg aagcgacccc tgaactgctg gcgctggttg gcgaagaagg
    13921 tgccgcagaa ccgtatcgct atctgatgaa aaacctgcgt tctcgcctga tggcgacaca
    13981 ggcatggctg gaagcgcgcc tgaaaggcga agaactgcca aaaccagaag gcctgctgac
    14041 acaaaacgaa gaactgtggg aaccgctcta cgcttgctac cagtcacttc aggcgtgtgg
    14101 catgggtatt atcgccaacg gcgatctgct cgacaccctg cgccgcgtga aatgtttcgg
    14161 cgtaccgctg gtccgtattg atatccgtca ggagagcacc cgccataccg aagcgctggg
    14221 cgagctgacc cgctacctcg gtatcggcga ctacgaaagc tggtcagagg ccgacaaaca
    14281 ggcgttcctg atccgcgaac tgaactccaa acgtccgctt ctgccgcgca actggcaacc
    14341 aagcgccgaa acgcgcgaag tgctcgatac ctgccaggtg attgccgaag cgccgcaagg
    14401 ctccattgcc gcctacgtga tctcgatggc gaaaacgccg tccgacgtac tggctgtcca
    14461 cctgctgctg aaagaagcgg gtatcgggtt tgcgatgccg gttgctccgc tgtttgaaac
    14521 cctcgatgac ctgaacaacg ccaacgatgt catgacccag ctgctcaata tcgactggta
    14581 tcgcggcctg attcagggca aacagatggt gatgattggc tattccgact cagcaaaaga
    14641 tgcgggcgtg atggcagcgt cctgggcgca atatcaggca caggatgcat taatcaaaac
    14701 ctgcgaaaaa gcgggtattg agctgacgtt gttccacggt cgcggcggtt ccattggtcg
    14761 cggcggcgca cctgcccatg cggcactgct gtcacaaccg ccaggaagcc tgaaaggcgg
    14821 cctgcgcgta accgaacagg gcgagatgat ccgctttaaa tatggtctgc cagaaatcac
    14881 cgtcagcagc ctgtcgcttt ataccggggc gattctggaa gccaacctgc tgccaccgcc
    14941 ggagccgaaa gagagctggc gtcgcattat ggatgaactg tcagtcatct cctgcgatgt
    15001 ctaccgcggc tacgtacgtg aaaacaaaga ttttgtgcct tacttccgct ccgctacgcc
    15061 ggaacaagaa ctgggcaaac tgccgttggg ttcacgtccg gcgaaacgtc gcccaaccgg
    15121 cggcgtcgag tcactacgcg ccattccgtg gatcttcgcc tggacgcaaa accgtctgat
    15181 gctccccgcc tggctgggtg caggtacggc gctgcaaaaa gtggtcgaag acggcaaaca
    15241 gagcgagctg gaggctatgt gccgcgattg gccattcttc tcgacgcgtc tcggcatgct
    15301 ggagatggtc ttcgccaaag cagacctgtg gctggcggaa tactatgacc aacgcctggt
    15361 agacaaagca ctgtggccgt taggtaaaga gttacgcaac ctgcaagaag aagacatcaa
    15421 agtggtgctg gcgattgcca acgattccca tctgatggcc gatctgccgt ggattgcaga
    15481 gtctattcag ctacggaata tttacaccga cccgctgaac gtattgcagg ccgagttgct
    15541 gcaccgctcc cgccaggcag aaaaagaagg ccaggaaccg gatcctcgcg tcgaacaagc
    15601 gttaatggtc actattgccg ggattgcggc aggtatgcgt aataccggct aatcttcctc
    15661 ttctgcaaac cctcgtgctt ttgcgcgagg gttttctgaa atacttctgt tctaacaccc
    15721 tcgttttcaa tatatttctg tctgcatttt attcaaattc tgaatatacc ttcagatatc
    15781 cttaaggaat tgtcgttaca ttcggcgata tttttttcaa gacaggttct tactatgcat
    15841 tccacagaag tccaggctaa accccttttt agctggaaag ccctgggttg ggcactgctc
    15901 tacttttggt ttttctctac tctgctacag gccattattt acatcagtgg ttatagtggc
    15961 accaacggca ttcgcgactc gctgttattc agctcgctgt ggttgatccc ggtattcctc
    16021 tttccgaagc ggattaaaat tattgccgca gtaatcggcg tggtgctatg ggcggcctct
    16081 ctggcggcgc tatgctacta cgtcatctac gggcaggagt tctcgcagag cgttctgttt
    16141 gtgatgttcg aaaccaacac caacgaagcc agcgagtatt taagccagta tttcagcctg
    16201 aaaattgtgc ttatcgcgct ggcctatacg gcggtggcag ttctgctgtg gacacgcctg
    16261 cgcccggtct atattccaaa gccgtggcgt tatgttgtct cttttgccct gctttatggc
    16321 ttgattctgc atccggtcgc catgaatacg tttatcaaaa acaagccgtt tgagaaaacg
    16381 ttggataacc tggcctcgcg tatggagcct gccgcaccgt ggcaattcct gaccggctat
    16441 tatcagtatc gtcagcaact aaactcgcta acaaagttac tgaatgaaaa taatgccttg
    16501 ccgccactgg ctaatttcaa agatgaatcg ggtaacgaac cgcgcacttt agtgctggtg
    16561 attggcgagt cgacccagcg cggacgcatg agtctgtacg gttatccgcg tgaaaccacg
    16621 ccggagctgg atgcgctgca taaaaccgat ccgaatctga ccgtgtttaa taacgtagtt
    16681 acgtctcgtc cgtacaccat tgaaatcctg caacaggcgc tgacctttgc caatgaaaag
    16741 aacccggatc tgtatctgac gcagccgtcg ctgatgaaca tgatgaaaca ggcgggttat
    16801 aaaaccttct ggatcaccaa ccagcagacg atgaccgccc gcaataccat gctgacggta
    16861 ttttcgcgcc agaccgacaa gcagtactac atgaaccagc aacgtacgca gagtgcgcgt
    16921 gaatacgaca ccaacgtgct gaagccgttc caggatgtgc tgaatgaccc tgcgccgaag
    16981 aaactgatca tcgttcatct gctgggtacg catatcaaat acaaataccg ctacccggaa
    17041 aatcagggca agtttgatgg caataccgat catgttccgc caggattaag cgcagaagag
    17101 ctggaatcat ataacgatta tgacaacgct aacctgtata acgatcatgt ggttgccagc
    17161 ctgattaaag actttaaagc ggcagacccg aacggattcc tggtttactt ctctgaccac
    17221 ggtgaagagg tttacgacac gccgccgcac aaaactcagg ggcgtaacga agacaacccg
    17281 acgcgccaca tgtacaccat tccgttcctg ctgtggacgt cggaaaaatg gcaagcgact
    17341 catccccgtg atttctcaca ggatgtcgat cgtaaataca gcctggcgga actgatccac
    17401 acctggtcag atttggcggg cttatcttac gacggttacg atccaacccg ttcagtggtg
    17461 aatccgcagt tcaaagaaac tacccgctgg attggtaacc cgtacaagaa aaacgcgctg
    17521 atcgattacg acactctgcc gtatggcgac caggtaggta atcagtaatt atcagccagg
    17581 cgctatttgc cggatgcgac gcttgacgcg tcttatccgg cctacagatc gcggcggtag
    17641 gctggataag gcgtttacgc cacatccggc aatacaactg ccgcaatgtt tacatcgaac
    17701 tcccctcagg ccacactttt caggtggcct tttttaataa ggccagttac tgttttcccg
    17761 tctacatacc taagggaata acatgtatca cgacgtcagc tacctgctct cccgcctgat
    17821 caacggcccg ctgtccctgc gccagattta ctttgccagc agtaacggcc cggtgcccga
    17881 tcttgcgtat caggtcgatt ttccacggct ggaaattgtg ctggaaggtg agtttgttga
    17941 taccggcgct ggagcaacgt tagttcccgg cgatgtgctg tacgttgccg ctggtggctg
    18001 gaatttccca caatggaaaa cacccgctac cacctttagc gtgctgtttg gcaaacagca
    18061 actcggcttc agcgtcgtgc aatgggatgg caaacaatat caaaatctgg cgaagcaaca
    18121 cgtcgcccga cgcggcccac gtataggttc ttttctgcta caaacgctca atgaaatgca
    18181 aatgcagccg caggagcagc aaacggcaag gcttatcgta gccagcctgc ttagccactg
    18241 ccgcaatttg ctcggcagcc aaatccagac cgcctcacgc agccaggcac tatttgaagc
    18301 tattcgcgat tatatcgacg aacgctatgc ctccgcgctt acccgcgaat ctgtcgcaca
    18361 ggcattctat atttcaccaa actatctctc gcacctgttt caaaaaacgg gggccattgg
    18421 ttttaacgaa tacctgaatc acacgcgact ggagcacgct aagacgttat tgaaaggcta
    18481 tgacctgaag gtaaaagaag tggcgcacgc ctgcggcttt gtcgacagca actacttctg
    18541 ccgtctgttt cgtaaaaaca ccgaacgctc gccgtcggaa tatcgccggc agtaccacag
    18601 ccaactgaca gaaaaaccga ctactccaga ataagatggg tttgctgcgg cgcagaaagc
    18661 actttgcgca ccgcgctcat tacccgctgc ggctcacgca ggaatgcgta gatgctgcat
    18721 tgcacatagc ggcaatgttc aaatcgctcg gcaccggcaa gctcgatatc cgtaatcaac
    18781 agagcaacat ccgcccgacg gatatcctcg tccgctaaac gattctccgt tcccagcgcg
    18841 ccctgagttt caatgctgac gccccacttc tctaactggc acaacttttc cagccgttcc
    18901 gccgccatat aagtatgcgc cacgccgctt acgcaggcgg ttactgccac caggtatgcc
    18961 attttaacct cccacggtaa cctgaaatcc ggcccgttct gccatttcgc gcatcgttgt
    19021 cacatcggct gacgacggcg caggcacctc tttcatcgac catgttttcc ccagcaggcg
    19081 gtatttcggt tcgccgtact gatgaaacgg taacagatgg atctgcctga ttttcagcgg
    19141 gatcagtaca tccagcgcct gctgcatatt ctcccggctg agcgtgaaac cagggatcaa
    19201 cggtaaacgc gggatcacgt tgacaccctc actcaccagc aaacgcagat tctccagcac
    19261 gcgtggcagg ttcatcttca ccacatcccg cgcctgagtc gcgtccataa tttttaaatc
    19321 gaacagcact tcatcgcaca atttcgccag tggtaatagc ttgctggctg gcgcgtctcc
    19381 ggcagtttca atggcgcatg acacgcccca caaccgcagc cgctgtaaaa aacgggtagc
    19441 aaactccgcc tgcattaaca cttcgccgcc agaaagcgtg acgccgccgc cggacgtgcg
    19501 aaagaaaatg tcatctttca tcacttcccg ttccagcgcg tcaaggctga tatcgcggcc
    19561 aatccgttca aacgccccgg aggggcattc atccgcatca cgcaaacatt tcgcacagtg
    19621 cagacatttc gcctctctgc gtaccgtctg gattttgccg gagatcgact ccggattagc
    19681 acaccacggg caaagatgcg gacagccttt aaaaaagacc accgtacgaa tgccctcgcc
    19741 gtcattcagt gaataacgct gaatgttgaa aatgcgcgcc acatcatcgc ggcgcgtttc
    19801 cacaacgtta cagctgatgc gctgtccggc ggatgatgtc atcctggatc tccttcgaca
    19861 gttcgacaaa gaaggcgctg tatccggcaa cgcgcaccac cagcccggca taatcttgtg
    19921 ggcgctgttg cgcttcccgc aacgtgtcgg cgttcaccac gttaaactga atatgctgca
    19981 acttaagctg ggtaaacgcc cgtaagaagt cggccagttt gcgtaatccc gcttcacctt
    20041 ccagggtcgc cggagtgaat ttcacgttca gcaacgtacc gttagacagc agcgtattat
    20101 cgagcttact gactgacttc agtaccgctg ttggcccttg tgcgtcctgg cccagcatag
    20161 gtgacaagcc gccgtctgcc agttgttccc cggcaaaacg accgtctggc gtcgcgccaa
    20221 ccaccgatcc caacgggacg tgagcagaaa cggtatacga tcccggcgtg aagtaaccgc
    20281 cgcgcgggtt ctggtatttt tccacttctt tgcagtagtg gcgtaacagt tcggcgctga
    20341 tgttatccac ctcgtcgata tcgttaccgt atttctcgaa gcggttaatt aagcgagcac
    20401 ggactttttc gccttctggc gttgcaaagt tggcttttaa taccgacagc aattcgtcaa
    20461 aacttaaacg ctgttgctca aaaaccatcc ctttcaacgc atggagagaa tcgctcaggt
    20521 tggcgatacc gatcccctgt acgccggaga agttataacg cgcgccgcca tcggtaatat
    20581 cgcgaccttt ttccagacaa tcgctgataa aagacgagag cagcggtaca ggtgcccagt
    20641 cgcgatggcc gatatcacaa atattgctgc cctcaaccat cagggtgatg tagtggctga
    20701 tcttggcgcg gatctgctcc agtaaacctt cataagtcag cgcagcattg ccttcatttt
    20761 catgcaggca gatttccatc actttcagca gattaaacat cgcgatgtca tgcaagccgt
    20821 aggttctgcc gggaatagat aattccacac agcccactac ggaatagtcg cgcgcatctt
    20881 ccagcgaaac gccacggttg aggaacgctg gtaccaccac ttcatcgtta aagatttgcg
    20941 gaataccggt gccgaggcga atggtttcgg cggttttcat caggaacggc gtgtcgatca
    21001 aggcgttagt gcgcacgccg aggttcggtt gcggtaattg cacgctttga taggcgtcaa
    21061 ggcaaaggaa cgaaagcacg ttcaccgcgc tacgtccgtt ctcggttaac ccgccgagca
    21121 gtgcggtata gccggtcggg aaacctgcga aataacgcgc gctgctggtg gagcgcaaca
    21181 gcacgatgtc gttgcatttc acccataaag attcgagcag ttctttcagg aacgccggat
    21241 cttcgccctg ggttaaagat gcctgataga acggcaacat atactggtcg aagcgcccta
    21301 aagacagcga actggcgttg gattcgtatt gcagaatgat gttcatgtac cagaataact
    21361 ggcacgcctg ccagaacgtc tgcggcttat gttcggcgtt atggcgagag atctccgcaa
    21421 tagtcagcag ttcttcgcga cgctgtgcat ctgtgcagtt tgccgccatc gtttccgcca
    21481 gttcggcgta acgcaaaatg tgtttctgcg aggcttccag cagtaacaac gcggcctgat
    21541 aaaagtgatt ctccggctgt tgctgacaat gttgctgcat ctgtgccacc agctcaccca
    21601 gaccgtgatt caacaggcgt gggtaatcaa taataatgtg cccctggcct ttatccgtct
    21661 ggttgatgct gaaaatctgc gtgttggtcg cggcttttac ttcatctgtc atctgcccgt
    21721 tgatgaaatc tttcatcgaa cgtttttccc agtacgggaa caactcttca cggtagatac
    21781 gtttatcttc ttcgctgata gcaaagcggt cctgcggacg cgtcgggaat tgatccagct
    21841 ctttcagcag ccagtaaggg tccatttccg gcgacataat cccggcgcgc ggttttacgg
    21901 tgcggttacc ggcaatcagt tcttcatcac gaatcgaaat ttcaacatgt tcaaggatat
    21961 acgctgttgc tttcgcccgg cgcaatatca ccggttcgcc ttcggtttgc cgatggctgg
    22021 cggtataaag cagcgcccgc tccagcgaga tttcacgggt attggcaaac agtgcagttt
    22081 tgaggcgaga gatacgattc gtcatgagga actcctgtgg gaaagcgaac ccgcccttgc
    22141 gagcgggaga agaacttaag cggtttgcgc caggtgagct tcaattttgc tcataatggc
    22201 gtcagcacgt tttactgcat cgctgatgtt gacgcgcacg atggtcttgc cagcaaaacg
    22261 ctcttcaaac ttgatgccga tatctttggt cagaatcacc atatcagcgc tcgccacatc
    22321 ttccgcagtc agttcatttt ccagaccaat tgacccctgg gtttctactt tcacttccca
    22381 gcctttcgct ttcgcggcac tttccagcgc ctctgctgcc atataggtat gggcaacacc
    22441 tgaaggacat gcggttactg caataatttt cgtcatggct cgttcctcaa ttaattaatt
    22501 tcaaaatcca aatccaggtc gtcttctttt tcatcagtcg aagacccatt tttacgcgcc
    22561 agacttttca gcacgttcac acaaacagcc gtcaccactg ctccgacagc cactgctgcg
    22621 atataaccca gcttgccttc aaccaccggc agcacaatca gaccacccca gcctgcatag
    22681 cactgcgcac cgaccagcgc cgcagttact gcaccacaaa ctgaaccgac catgatggaa
    22741 ggaatgacac gcagcggatc ggcagcggcg aaaggaatcg ccccttcagt aacgccaaca
    22801 cagcccatca ccagtgccgc tttgccggtt tcgcgctctt ctgcggagaa atttttacga
    22861 ccaatcagcg tcgccagccc catccccagc ggcgggatac aaatacccac ggcagcgatg
    22921 gccaccacgg tataaacacc ctgagcaacg caaatcagca tgaaggcata ggccactttg
    22981 ttaaccggac cgcccatatc gaacgccagc atcagaccca tgatcaccgc cagcataaca
    23041 atgctgccct gctgcatccc ctgaagccac tgagtcaggc tgttggtcaa cgcccctaca
    23101 ggctcgccca agccccacat catgatgcct gcggtaatca gtgtgccgac gataggaata
    23161 atgaagatag gcatcaccga gcgcagaact ttatgcaccg gaattttctt caggtaatgc
    23221 accacgatgc cgccgataat cccggcgatc agtgcaccaa agaagcccgc accaaagctg
    23281 ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc aatggaataa
    23341 ccgatgtacg ctgcgaggaa aggcaccatc agcgtcaagc ccgcaacgcc gatatcaaac
    23401 agttttttca gatttggatc ggctacggca tccggcactg cgcctttgcc atacaacatg
    23461 acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg tgaaacgccc
    23521 gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat aagctgctcc
    23581 cgggcttgcg aaagcccatg tccatatttt tgatggagga aacaataggc aatcgcaggc
    23641 ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag tacaaaaatg
    23701 cgatccgcct cataacttgc gataaaccgg aagcatgccg caaaacagcg tcttgtgaga
    23761 cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt tgtcacatgg
    23821 caggccgttg ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga gagaggcaat
    23881 ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc gtccggcaag
    23941 ccacgttgaa acgctgtgta atactttttc atcacaaatt gagtggcata acctgcgcac
    24001 tgaccgcaag ggcaacgcca aaagcgtcct tgcgctgatt ggcaccgata cgctggcggg
    24061 cgataactgc cagttactga tttccggggc cgacgaacag gaagcgcacc agcgtttaag
    24121 ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag ttaaatctga
    24181 cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta tccgcgcccg
    24241 caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt tagatctcaa
    24301 tgcgctgggt aatcttcccg cagccaaagg cgttgacgcc gagcaatccg cactggaaaa
    24361 cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg acggtgccac
    24421 cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc gcgaacattt
    24481 actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcacca gtgcgaatca
    24541 cttttgcgaa gagttcgccc gttccagcag cagctacctg caagaacgtg ccctggacgt
    24601 acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct tcccggcacc
    24661 gggcaaactg acgcagcccg ccatttgtat ggctgatgaa ctgactccca gccagttcct
    24721 cgaactggat aaaaatcacc tcaaaggtct gttactgaaa agtggcggca ccacctcaca
    24781 taccgtaatt cttgcccgtt cgttcaacat tccgacactg gttggtgtag atattgatgc
    24841 ccttactccg tggcagcatc aaacgattta tatcgacggc aacgctgggg cgattgtggt
    24901 tgagccagga gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac aggacgccct
    24961 gcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg gtatccgcat
    25021 tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg gcaatggtgc
    25081 ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca gtgcaccggg
    25141 cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg gacgcagcat
    25201 tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctaa acattcccgc
    25261 agaggcaaac ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat acgcgtcgtt
    25321 atttaccaca caactacgct cgatcctgcg cgcctccgct cacggcagcc tgaaaatcat
    25381 gatcccgatg atctcctcaa tggaagagat cttatgggtg aaagaaaaac tggcagaagc
    25441 caaacagcaa ctacgtaacg aacacattcc gtttgatgag aagatccagc tcggcattat
    25501 gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga ttgatttctt
    25561 tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg ataacgctaa
    25621 ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg attacgccgt
    25681 gcaggcggtg catcgccagg gcaaatggat tggtctgtgc ggtgagctgg gagcgaaagg
    25741 ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga gcgcaccatc
    25801 aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc gcaagttgct
    25861 caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg cgcaattccg
    25921 catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg aaagtgactg
    25981 gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg cgggccgctg
    26041 ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt tctctaccgg
    26101 tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc aatccaccat
    26161 cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc aattcatcat
    26221 tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt tctcgcgcct
    26281 cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg cctctgccga
    26341 cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat catggaactg
    26401 tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt ccccattgcc
    26461 ggggtgacaa ctaacccgag cattatcgct gccagcaagg agtccatatg ggaagttctg
    26521 ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac tatgagtcgt
    26581 gacgcgcagg gtatggtgga agaagcgaag cgcctgcgcg acgccattcc gggcattgtg
    26641 gtgaaaatcc cggtgacttc cgaaggtctg gccgccatta agatgctgaa aaaagagggt
    26701 attactacgc ttggcaccgc agtgtacagt gctgcacaag ggttactggc cgcgctggca
    26761 ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg ggacggcatt
    26821 cgtacggttc aggagctgca agcgctgtta gaaatgcacg caccagaaag catggtgctg
    26881 gcggccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg atgtgaatcc
    26941 atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt agagtcagct
    27001 atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct ctaaaggagc
    27061 aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg atgtgattaa
    27121 tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg gtgacaaatt
    27181 tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg gactggtagt
    27241 agaaattgcg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc tgcgtggcat
    27301 cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa ccctcgatac
    27361 tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga ctatcgcctc
    27421 taccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg agtttgaccg
    27481 ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa tcgtcgcggg
    27541 cgcacctgca cgtctgttag cagcgggtat cggcgatgcg ctggcaacct ggtttgaagc
    27601 gcgtgcttgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca cccaggcggc
    27661 gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa aagcgatgct
    27721 tgccgccgaa cagcatgtag taaccccggc gctggagcgc gtgattgaag cgaacaccta
    27781 tttgagcggc gttggttttg aaagcggcgg gctggcggcg gctcacgcgg tgcataacgg
    27841 cctgaccgct atcccggacg cgcatcacta ttatcacggt gaaaaagtgg catttggtac
    27901 gctgacgcag ctggttctgg aaaacgcacc ggttgaggaa atcgaaaccg tagctgcact
    27961 tagccatgcg gtaggtttgc caataactct cgctcaactg gatattaaag aagatgtccc
    28021 ggcgaaaatg cgaattgtgg ctgaagcggc atgtgcagaa ggtgaaacca tccacaacat
    28081 gcctggcggc gcgacgccag atcaggttta cgccgctctg ctggtagccg accagtacgg
    28141 tcagcgtttc ctgcaagagt gggaataacc tactccaaac tcccggcttg tccgggagtt
    28201 tgaacgcaaa attgcctgat gcgctacgct tatcaggcct acgcaatctc tgcaatatat
    28261 tgaatttgct tgttttttag gccagataag gcgttcacgc cgcatccggc actctgtgcc
    28321 agtaatctga aactcccacg ttgttcagga gttttttatc cgcttctgga cattttctct
    28381 acagaacaat cgttatcccg gaaggtcgaa tctgtgactc ttattttcac ggacgaacca
    28441 gatgaaagct tcactggcgc tgctcagcct gctaacggca tttaccagcc actctttaaa
    28501 atctcccgcc gttccaccaa ccgtggtaca aatccaggcc aataccaacc ttgccatcgc
    28561 agacggtgcc agacagcaaa tcggcagcac gctattttac gatccggcgt atatgcagct
    28621 tacctatcct ggcggtgatg tcccgcaaga acgcggggta tgttccgatg tggtgatccg
    28681 tgcattgcgc agccagaagg tcgatttgca gaaactggtg cacgaagata tggcgaagaa
    28741 ttttgccgag tacccgcaaa aatggcagtt aaagcgcccg gacagcaaca tcgatcaccg
    28801 tcgggtgcct aatctggaaa cctggtttac ccgccacgat aaaacacgcc ccatcagcaa
    28861 gaaccccagt gactatcaag cgggcgatat tgtctcctgg cgattagata acgggctggc
    28921 acatattggg gtagtttcag atggcttcgc ccgcgacggt acgccgctgg tgatacataa
    28981 cattggcgca ggtgcgcagg aagaagatgt gctgtttagc tggcgcatgg tggggcatta
    29041 tcgttatttt gttaaataag cgatcgcccc acatgaagaa aaaattcaga tcctttttac
    29101 actaacggct tcttttttct tacggctcac cagcgccagt gccatcacaa tcagcacaat
    29161 accgctacct tcaacggccc ccggattctc gccgagcagc caccaggaaa agagtacgcc
    29221 gcaaacggga acggccagtg tgcttaagct ggcaatactg gcaggcaagt ttttcaatac
    29281 aaacaaccat aagctccacg ccagtgccgt cgccagaatc gcactgtagg ccagcgccca
    29341 gaacacggtg ggctgccagt caatttcacg ttgcggcacc agtaaagcga ccacactcat
    29401 caccagcgcc gcatagagca tctgccagga tgttaacgac aataaatcca cgcgcggatg
    29461 acgggcatac agacgtttag caacaatcgc gctcgccccc caactgacgc cggagaggat
    29521 tgccagcatg gcacttttca tcgaagagaa atcgagttgc cacggctgca acaccaaaaa
    29581 taagccgaaa gcggcaatca gtatcgcgaa atattgccca cgtcgcaggc gttcaccgag
    29641 aaacaacgcg gcgaaaatca ccacccagaa cggcatggta tagcttagga tcgccacctt
    29701 ccccgctccg ccgctgacca gcgcccactg caccaggcca accatcccgc aggtttgtaa
    29761 cagggcaatg actaaggtgt atttaaacgg tgtcgggcgc attccgcgac cacgtaataa
    29821 aaggacgatg aataaaacga gagcgccgaa aatgcagcgt aaggcggtaa agtcgaaggc
    29881 accgatgtag ctggtgactt gcttcatgaa aatccagcta taactccaga taagtgtgag
    29941 cacaaccagg ccactgattg ccagtgggtt gctctttcct gcggcagaca tgagagatcc
    30001 agtgtgtagt tttcctgtgg acactatacg ccggtaaaga aacttcagcg actgcctgaa
    30061 aggcagtcgc tgaagaaggt cagattacag caggtcgaaa cggtcgaggt tcatcacttt
    30121 cacccatgcc gccacgaagt ctttaacaaa cttctcgtga gcatcgctgc tggcgtaaac
    30181 ttcagccacc gcacgcagga cagagttaga accaaatacc agatccgcac ggctggcagt
    30241 gtatttcact tcgccggttt cgcggtcacg gccttcaaac agctctttcg attcgtcggt
    30301 cgctttccac tcgtaacgca tatccagcaa gttcacgaag aagtcattgc tcaatacgcc
    30361 aacgcggtca gtgaagacgc cgtttttgct gccatcgaag ttggcaccca gtacacgcat
    30421 cccgcccacc agcgcagtca tttccggcgc ggtcagcgtc agttgctgtg ctttgtcgat
    30481 cagcagtgac tcggtggtgg aaacgtccag acgagcgcga tagttacgga aaccgtcagc
    30541 aattggctcc agcagctcaa acatctcaat gtcagtctga tcctgacgcg catcaacgcg
    30601 acccggcgca aacggtacat gaatgctcaa acctgcggcg cttgcggctt tctcaacacc
    30661 aaccacacca gccagcacga tgatatccgc cagcgaggct ttaccagact ctttctggat
    30721 tttctccaga acaggcagag cacgaacggc tgcggcgttc acatcccagt cgcgctgcgg
    30781 cattaatgcc agacgcgcac cgttggcacc accgcgtttg tcgccaccac ggaaggtaga
    30841 agcagatgcc caggccaccg ataccagctc actaacagac agaccagaat ccgcaatcgc
    30901 gaatttcaga tcgataatgt cctgctcggt cgggttgtag atcggctgcg gcagcggatc
    30961 ttgccagatc agatcttctt tcggcacttc cggcccgatg tagcgagatt tcggccccat
    31021 atccctgtgc gtcagtttga accaggcacg ggcaaaggct tcgttgaacg cctgcggatc
    31081 gttgaggaaa cgacgagaga tcttctcgaa ctcaggatca aaacgcagcg tcaggtcggt
    31141 caccagcatt gtcggtttac gtttcttcga cggatcgaac ggatccggga taatttccgg
    31201 tgcgtctacc gcttcgaact ggattgctcc agccggactg cgggtctgta cccactcata
    31261 cttgaacaga ttctcgaaga aatagttgct ccactgggtc ggcgtctggg tccagactac
    31321 ttccagacca gaggtaatgg catctgcgcc aacgccgctg ccgtaagtgc tcgcccagcc
    31381 taaaccttgt tcttcaatcg gtgcagcttc tggatcagga cctacgtttg atgtcggacc
    31441 ggcaccgtgg gttttaccca gcgtatgacc acccgcaatc agcgccacgg tttcttcgtc
    31501 gttcatgccc atgttgccga aggtcgcgcg gatagctgct gccgcagaaa gcggttcgcc
    31561 gctgtgatcc gggccttccg ggttaacgta aatcaggccc atctcggttg cacccagcgg
    31621 tgctttcgcc agcgcttccg gatgacggtg agtcagccag gctttttcat caccccagtt
    31681 aacatccaga tccggttccc agacgtcttc acgaccggca ccaaaaccga aggtacggaa
    31741 gccggagttt tccagcgcca cgttacctgc gaggataaac aggtcggccc aggagatttt
    31801 ctgaccatat ttctgtttga ttggccacaa caggcgacgc gctttatcga ggcttacgtt
    31861 atccggccag gagttcagcg gtgcaaaacg ttgctgacca cgacccgcgc cgccgcgtcc
    31921 atcgattgaa cggtaagtac ctgcgccgtg ccaggccata cgaataaaca gaccggcgta
    31981 gctgccccag tcggctggcc accacggctg agattctgtc agcagggctt tcagatcttt
    32041 tttcaggccg tagtaatcta atttgctgaa ttctttgcgg tagtcaaagt cctcacccag
    32101 cgggttagaa cgattagaat gttggtttaa caggtcaaca cggagttgat ttggccacca
    32161 gtcgcgagtg gttgtgcccg cccccgcgct ctggtcgtga ccgccctgat ggaacgggca
    32221 tttgccagtg gctgtcgtgt tatggatatc gtctgacgtg ctcatcaatg tgctcccctc
    32281 tacagtgtta ccgttacgat acacagcgtt agagagaagt tataattgaa ttaatccacg
    32341 gatgcgatag ttgagatctt acatattgtt ggttaaagag atgtaggtca aattgatcgt
    32401 aattaagctt ttttgattat gtccgaattc ggacaaatgc tttataaaaa agggttttag
    32461 cttcatatcc ttcaagtaat gagggagaaa acaaggctga caaccttatt ttcccgccct
    32521 catttcgagg cagcattttg tgctctgttt aaaatttgtg atcactgtgt gattttcaca
    32581 aaagccacac tatttataaa ccaggtcgaa cacccagcgt atggcaaatc gcgtaactca
    32641 tttcagcgcg gttaagcgta tagaagtgga aatctttcac tccttcacgg cttaaaatct
    32701 tcaccatatc catggcaata ttcgcgccca ccagtttgcg ggtttcggca tcatcatcca
    32761 ggccgtcgaa catttgcgcc atccacgccg gaatacgcac gttggtcata tcggcaaatt
    32821 tcttcgcctg tttaaagtta gataccggca aaattcccgg aataatttcc acatcaatgc
    32881 ccgccgatac acagcggtca cgaaaacgca ggtagctttc gacatcgaag aagaactgag
    32941 taatcgcgcg gttggctccg gcatcgactt tgcgtttcag attaagcaaa tccgcctgag
    33001 cgctttttgc ttccgggtga acttccggat acgccgccac ggagatatcg aaatctgcca
    33061 cttcttttaa cagcgtcacc aggtcagaag catacatttc cggcttacca cttcccggcg
    33121 gcagatcgcc acgcagcgcc acgatatgac gaataccgtt attccagtag tcgcgtgcaa
    33181 tggtgcgcag ctcgtcgggc gtcgcatcaa tgcaagtaag atgcggtgcc gcttccagac
    33241 cagtgcgatc tttaatgcct ttaataatgc tgtgcgtacg gtcgcgctcg ccggagttcg
    33301 cgccataggt caccgataca aacttcggtt tcaggctgct aaggcgatcg atggagttcc
    33361 acagggtctg ctccatttca ctggtacgcg gcgggaaaaa ctcgaacgaa acgttaatct
    33421 gcccctggac ttctgccagg ctctgattca gggcatcccg ctggctggcg tgaaaaaagc
    33481 tcatacctta cctcatcaat cgcttgtagt tatatgttgt gttgtgcaca tctatccatt
    33541 tagacgtcca gatgtaaaga tgaaggaaaa gccgaagggc gtcaaccgaa aaatgaccaa
    33601 tgatggtgag gaaaaatcag ggaagatgaa aaaacttcag gatggtaaga aaaagaaaat
    33661 ccggtatgca aagcaaaccg gactgcaaat ataaactaac catgtactta ttgcttcttg
    33721 ccggattcgt cgtaaacgct tcatccggcc taatcctatg ataattcaat atatttcatt
    33781 agaaaccata gacccgaaaa catgagtacc gggcattatt aaatttctga aattacaaca
    33841 actgtgccag ccggttgata tccgactgaa tcgccccggc ggtgacgtcg cgcccagcgc
    33901 caggcccacg gatcaccagc ggattatcgc ggtaccagcg gctttcgatg gcaaagacgt
    33961 tatcgcatgg cagcagtgat gccagcggat ggtcttcacg caccgcttcc acgcccacac
    34021 gcgctttgcc attggcatcg aaacgcgcca cgtagcgcag caccagcccc atttcgcggg
    34081 ccgcttccag ccgttgcacc atctgctcgt taagttcatc gccattttca aagaaatggt
    34141 cgatgctgcc gccttcgcaa tgggtaggca ccagcgactc cacgcgtacc tgatccggtt
    34201 cgatgttgta acctgcttca cgcgccagaa tcaccagctt gcgcatcacg tctttgccag
    34261 agagatcgtc acgcgggtca ggttcggtta agccctgctg ccacgcctga tccaccagct
    34321 cggtaaacgg cacgctacca tcgaattgca ggaacaacca ggagagcgtg ccggagaaga
    34381 tcccgctgat cgacaaaata gtatcgccgc tgtcgatcag atcgcgcacg gtgtggttga
    34441 tcggcaagcc cgcaccgacg gtggcattgt acagccagtg acgcccggtt ttttcgaagg
    34501 cgtcgtggat ctggcgatat ttattgctgt cgctggctcc cgccagtttg ttggcgctga
    34561 taacgtggaa accgtggctg gcgaaatcaa gatactgatc agcaagctgc tggctggcgg
    34621 taacgtccag caccactaaa tcatcatacg gatgggcgcg catccacagg aacaacgact
    34681 cttcatcctg ctcaaccgct tcatcgttga agaaggctaa cgcgcggctg gcgtccagcc
    34741 cgtcatagct caacaggctg cggcggctgt ccaccacacc tgccagcaca aactcaaagc
    34801 cggtacgtgc cgaaagcgtg ctctgctcac gggcgaacag ttccagccaa cgggaaccga
    34861 tattgccctt accgaacaat accaggccga tgcgtttttc tgcgcggaag acggactgat
    34921 gcagcccctg aatcaggctt tcggtcgggc cggtgcgcag tactgccacc aggctgatgc
    34981 cgtcatcgga ctgccaggta aattcgaccg gctggccttt cagttgctgc cagaagcggt
    35041 ggcaatgcag cgggttacgg gtgacgcctg caccgaccat cgccaccagc gccagcccct
    35101 gacgcaggcg cagttcgcca ggtaatcccg cttcgtcgag gattttcagc gcactgtcgg
    35161 ccacttctga ggtgtagcaa aattgcagca actggcgatc gttatgtacg ccaaccgcca
    35221 gcgggcgtac ctgcgcgcgt ttcaggattt ggtcgatctc tttatgcgcc agtttgaaat
    35281 cctgactggc gggcacctga aactcaatca aacagacatc atcgtggctg gtgacaatac
    35341 gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt ggaaccttga tccggcgtgt
    35401 agctacagcg cagttgcagg tcgatttcgc tgccagaaac cggctgtaaa gtacgggcgt
    35461 gaagaacggg agccgccagg cgcgccagtt cgctggcctc atccagacgc agcaacggca
    35521 gcaggcaggc atctttcact ttacgcgggt cggcactgta taccccggcg acgtcgctcc
    35581 agatggttac gcgagaaaca cccgccagcg caccgatttg tgtcgcggaa tagtcggaac
    35641 cgttacgccc cagcagcacc gtttcactgg cgttgttgcg gctgataaat ccggtcacca
    35701 ccagacgttt gcccggatgt tgcaccagca gctgttgcag caacgggtaa gaaagccctt
    35761 catcaacctg cggttgtgcg gcgcgttcag cgcgtaaaaa ctcgcgggca tcaagccagg
    35821 ccgctggcag cccttgttga ttaagtaccg cagacatcag gcgtgccgac catacttccc
    35881 cgtggcccac cacttccgca tacactgcgt cgttaatacc gctgtcgagc agcgccgcca
    35941 ggcgttccag atcgctgaca aaagcgctaa tgaggctatc ggcttcttca gcgggtagca
    36001 gaccgctaat cagatcgcac tgatagcgac gcagcgtttg ttgaacctga tgcgcagaga
    36061 gacgatcggt ctggcttagt ttcaaccagt taatcaactg gttagtggtg ctaccggcgg
    36121 cggaaaccac catcatatcg tcaggctgag agtactccgc cataatgccc gcgacacgca
    36181 aataacactt cacatcagcc agactactgc caccaaattt atgcagctga cgacctttcg
    36241 cccctgcctg cgcaatcaca ctcattttta ccccttgttt gcagcccgga agccattttc
    36301 caggtcggca attaaatctt cgccatcttc aataccggtg gagatacgca gcagcgtctc
    36361 ggagatcccg gcggcagcac gcgcttctgg tgccatgcct gcatgtgtca tggttgcggc
    36421 gtgagagatt aaactttcca ctccccctaa tgattccgcc agcgtaaaca acgacagccc
    36481 gcccaggaaa cgacgcagcg tctgctcatc gccatccagt tcaaaactca acattgcgcc
    36541 aaagcctttt tgctgacgcg cggcaatttc atgcccctga ttttccggca gcgacgggtg
    36601 atacagtttt ttcaccaacg gctgggtttg cagatatttc acaatcgcct gcgcgttgcg
    36661 ctgcgccagc tccatacgcg gcaccagcgt tcgcaacccg cgtagcagca gatagctgtc
    36721 aaacgcgccg cccgtcacgc caatattgtt tgcccaccag gcaagttcag tgacaacgtc
    36781 cgggtcttta gcaatcacca cgccagccac cacgtcagag tgaccgttca gatatttcgt
    36841 acatgaatgc aacaccagat cggcacccaa tgccagcgga ttttgtaatg ccgggcttaa
    36901 gaaggtgtta tccaccacgc tcaccgcccc gacttccctt gccagatggc agattttcgc
    36961 aatatccacg acgcgtaaca atggattact tgggctttct accagtacca gtttgggttt
    37021 ttctgccagc gctgcccgta atgcctgttc atcgccttga tcaacaaaca acacgcgata
    37081 gcaaccgcgt ttcgccagac tgtcgaacag gcgatagcta ccgccgtagc agtcgtgcgg
    37141 cgcaaccagc agatcgccag gtttcaaaaa gacggtcgtt accaggtgaa tcgcggacat
    37201 gccggtatta gtaagtactg cgccagcacc gccttccagt tctgccagcg cacgctgaac
    37261 cacatcgcgc gttgggttgc cgcgacgcga gtaatcatgc gcgcgcggtt cattaaatcc
    37321 ggtaaagtta taggtgctgg aaagatggat gggagggaca acgcaaccat actgttcgtc
    37381 gtcatttaac ccgctacgca ctgcgatggt ggcctgttta cgcgtcatgt gatgaagttc
    37441 cctgggcttt gtcggtgaaa tgtcaggcac cagagtaaac attgtgttaa tggacgtcaa
    37501 tacatctgga catctaaact tctttgcgta tagattgagc aaatcccaaa tagccgttaa
    37561 aattatatgc attatcacgc cgacaggtgc attacacgat gtcacggtaa cgccggtacg
    37621 gtaaactatg cgggtttacg gtcagtaccc acataaattg tgtggtctgg tctcaattta
    37681 ttgacgaaga ggattaagta tctcatggct gaatggagcg gcgaatatat cagcccatac
    37741 gctgagcacg gcaagaagag tgaacaagtc aaaaagatta cggtttccat tcctcttaag
    37801 gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg tgaacaacct gcgtcacgct
    37861 accaacagcg agctgctgtg cgaagcgttt ctgcatgcct ttaccgggca acctttgccg
    37921 gatgatgccg atctgcgtaa agagcgcagc gacgaaatcc cggaagcggc aaaagagatc
    37981 atgcgtgaga tggggattaa cccggagacg tgggaatact aaagcggaaa tgagcggcgc
    38041 ggagtggaat cgcctgatgc gctacgctta tcaggcctac gtcatattgc aatttattga
    38101 atttgcacga acttgtaggc cggataaggc gttcacgccg catccggcat aaacaacgag
    38161 cacgttgtct gcgacccacc gctttttata catggacgtt taactatgaa aaacaggctg
    38221 ctgatcctca gcctgctggt ttctgtacct gcctttgcct ggcagccgca aaccggcgac
    38281 atcatctttc aaatctctcg ctcatcgcaa agtaaagcga tccaactggc gacccattcc
    38341 gattacagcc acaccggtat gctggtgata cgcaacaaaa agccctacgt ttttgaagcg
    38401 gtcggcccgg tgaaatacac cccgctcaag cagtggatcg cccatggtga aaagggcaaa
    38461 tacgtcgttc gccgcgttga aggcggactg agtgttgaac aacagcaaaa actggcgcaa
    38521 acggcaaaac gttatctcgg taaaccgtat gatttcagct tctcatggag cgacgatcgc
    38581 cagtactgtt cggaagtggt gtggaaggtt taccagaacg cgctgggaat gcgcgtgggc
    38641 gaacagcaga agttgaaaga gtttgatctc agcaacccgc tggtccaggc gaagctcaaa
    38701 gaacgctacg gtaaaaatat tccgcttgag gaaacggtag tttcaccaca agccgttttc
    38761 gacgcgccac aactcaccac ggttgccaaa gagtggccgc tgttttcgtg gtgatacgac
    38821 caaaattcag gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc ggtcttattt
    38881 gctgcccggg atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg tagcaacatc
    38941 acgctgtttg ccagtgaaga acgggtggca cttgctgcac acgtcgaggt tcaggtcatg
    39001 accaacggtg gagcggattt tcattacgtt accgcaagag cagctagcag taatttcttc
    39061 gtatttcggg tgaatatctt ttttcatggg aaaacctcgg tttaaggccg cgtcgctctt
    39121 ccagccctaa cgccagacac cacgcgatgt taaaagtata gcttcaatac gatcatttcg
    39181 tacgaaggcg cgaaatcata cagaaattaa ccagcgtatg caaactgatc cgcactcttc
    39241 tacggcaatg tgtatactaa cccaccgaat ttcaagtcag gatgatgcta tgcccgttgc
    39301 ccacgttgcc ttgcccgttc cgcttcctcg tacctttgac tatctgctgc cagaaggcat
    39361 gacggttaaa gccgggtgtc gcgtgcgcgt gccgtttggc aaacagcagg agcgcatcgg
    39421 ggttgtggtg tcagttagcg atgtcagtga gctgccgctt aacgagctga aagcggtagt
    39481 ggaagtgctg gacgttgagc cggtatttac tcactccgtc tggcggttac tgctctgggc
    39541 ggcagattac tatcatcatc cgattggcga tgtgctgttt catgccttgc cgattttact
    39601 gcgccagggg cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca ctgaacaagg
    39661 ccaggcggtg gatctgaaca gcctgaaacg ctcccccaag caacaacagg cgctggcggc
    39721 gttacggcaa ggcaaaatct ggcgcgacca ggtcgccacg ctcgaattta atgatgccgc
    39781 gttgcaggcg ctacgcaaaa aaggtctgtg tgatttagca agtgaaacac cagagtttag
    39841 cgactggcga acgaactatg ccgtttctgg tgagcggttg cgattgaata ccgaacaggc
    39901 caccgccgtt ggcgcaattc atagcgcggc agatactttt tctgcctggc tgctggcggg
    39961 cgttaccggt tccggtaaaa cggaggttta tctcagtgta ctggaaaacg tgctcgctca
    40021 gggcaaacag gcgctggtga tggtgccgga aatcggcctg acaccgcaaa ctatcgcccg
    40081 ttttcgtgaa cgttttaatg cccccgtgga agttctgcat tccggcctga acgacagcga
    40141 gcggctttcg gcgtggttga aagcgaaaaa tggcgaggcg gcgattgtga tcggcacccg
    40201 ctccgcgctg tttacgccgt ttaaaaatct cggcgtgatt gtcattgatg aagagcacga
    40261 cagctcctac aagcagcagg aaggctggcg ctatcatgcc cgcgacctgg cggtgtatcg
    40321 tgcgcatagc gagcaaatcc cgattattct tggctccgca acgcccgcgc tggaaacgtt
    40381 gtgcaacgtc cagcagaaaa aataccgcct gctgcgcctg acgcgccggg cggggaatgc
    40441 gcgtccggca attcaacacg tgctggattt aaaaggtcag aaggtgcagg caggtctggc
    40501 tccggcgtta atcacccgta tgcgccagca tttacaggcc aacaatcagg ttatcctctt
    40561 tcttaatcgt cggggttttg cgcctgcgct gctgtgccac gactgcggct ggatagccga
    40621 atgtccacgt tgcgatcact actacacgct gcatcaggcg caacagcatt tgcgctgtca
    40681 ccactgtgac agccaacgcc cggtaccgcg ccagtgccct tcctgcggtt ccacgcacct
    40741 ggtacccgtg gggctgggca ccgaacagct tgaacagacg cttgcgccgc tgttccctgg
    40801 tgtgcccatt tctcgtatcg accgcgatac caccagccgc aaaggagcgc tggaacaaca
    40861 actggcggaa gtgcatcgcg gcggcgcgag gatcttgatt ggcacgcaaa tgctggcgaa
    40921 aggtcaccac tttccggatg tgacgctggt agcgttactg gatgtggacg gcgcgctgtt
    40981 ttctgccgat tttcgctcgg cagagcgttt tgcccagcta tacacccagg tcgccggtcg
    41041 tgccgggcgt gcgggtaaac aaggcgaagt ggtgctgcaa acgcatcatc cggaacaccc
    41101 gctattgcaa acgttgctct ataaaggcta cgacgctttt gccgaacagg cactggctga
    41161 gcgacgcatg atgcagctgc cgccgtggac cagccatgtg attgtgcgtg cggaagatca
    41221 taacaatcag catgcgccgc tgttcctgca acaactgcgt aatctgatcc tctccagccc
    41281 gctggcagac gacaaattgt gggttctcgg tccggttccg gcactggcac ctaaacgtgg
    41341 cggtcgctgg cgctggcaga tattgttgca gcacccttcc cgcgtgcgct tgcaacacat
    41401 cattaacggt acgctggcac tcatcaatac aataccggat tcccgtaagg tgaaatgggt
    41461 gctggatgtt gatccgattg agggttaaac cgctcacgat gcgaggcgga tcgaaaaatt
    41521 caatattcat cacacttttc atgaaaattc tgtaaccgtt ttcacgcgct atctgctaaa
    41581 aatgttgccg atgtgaagta aacatggatg tagtacgcct gacgtgccag gcgaggagtg
    41641 agtgtgaaag cgaagaagca ggaaactgcc gcgaccatga aagacgttgc cctcaaggca
    41701 aaagtctcta cagcgactgt ctcccgagca ttaatgaatc ccgataaagt ctcccaggcc
    41761 acccgtaatc gggttgaaaa agcggcccag gaagtgggtt atttaccgca gcctatgggg
    41821 cgcaacgtca agcgtaatga atcccgcacc attctggtga ttgtcccgga tatctgcgat
    41881 cccttcttta gcgaaattat tcgcggtatc gaagttacgg cggcaaatca cggatatctg
    41941 gtgctgattg gcgactgtgc gcatcaaaat cagcaggaaa aaacctttat cgatttgatc
    42001 atcaccaagc aaattgatgg catgttgctg ctgggttcaa ggctgccgtt tgatgccagc
    42061 attgaggaac agcgtaatct gccgccgatg gtgatggcga acgaattcgc accggaactg
    42121 gagctaccta cagttcatat cgacaatctg accgccgcat ttgatgcagt aaattatcta
    42181 tatgagcaag ggcataaacg gattggctgt atagccggtc ccgaagagat gccgctgtgt
    42241 cactaccgcc tgcaaggtta cgttcaggcg ctgcgtcgct gcggcattat ggttgatccg
    42301 caatacatcg cccgtggcga cttcaccttc gaagccggaa gcaaagcgat gcagcagctg
    42361 cttgatcttc cacaaccgcc tactgctgtc ttctgccata gcgatgtgat ggcgctcggc
    42421 gcactttctc aggcaaaatg ccaggggctg aaagtcccgg aagacctttc cataatcggt
    42481 tttgataaca tcgacctgac gcaattttgt gatccgccgc tgacaaccat cgcgcagccg
    42541 cgttacgaaa tcggtcggga agctatgctg ttattgcttg atcaaatgca ggggcaacac
    42601 gttggcagtg gctctcgttt aatggactgc gaacttatca tccggggatc aacacgcgcg
    42661 ttaccttaaa gtaaaccttt aagactgccg tgactggtca aaggcccgcc gcttaagtaa
    42721 catggcgggc tgacgaacga ataaatacag cgaaacgata gtggcacaac gagattatgt
    42781 acgccgcagc caaccggcac cttcgcggcg aaaaaagagc acctcacgga aaaagcaacg
    42841 aaatctgcct gcggtttctc ccgctatggt cgctattgct gccgccgttc ttgtgacctt
    42901 tatcggtggt ctgtacttca ttacgcatca caagaaagaa gagtccgaga cgctgcaaag
    42961 ccagaaagtg accggtaacg gactaccacc aaaaccagaa gaacgctggc gctacattaa
    43021 agagctggaa agtcgccagc cgggtgtgcg tgcgcccaca gaaccttctg ccggtggtga
    43081 agtgaaaacg ccggagcaac tgacaccaga acaacgtcag cttcttgaac aaatgcaggc
    43141 tgatatgcgc cagcagccaa cgcagctggt tgaagttccg tggaatgaac agacgccgga
    43201 acagcgtcag caaacgctac aacgccagcg tcaggcgcag caattagcgg agcagcaacg
    43261 cctggctcag cagtcgcgta cgactgaaca aagctggcag cagcagacgc gtacgtcgca
    43321 agccgcgccg gtacaggcgc aaccgcgcca gtctaaaccg gcttctactc agcagccgta
    43381 ccaggatctg ctgcaaaccc ctgcgcacac gaccgcgcag tcgaagccgc agcaagctgc
    43441 gccggttgct cgtgccgctg acgcgccaaa accgacggcg gagaaaaaag acgaacgccg
    43501 ctggatggtg cagtgcggtt cgttcagagg cgcggaacag gcagagacgg tacgtgctca
    43561 gctggcgttc gaaggatttg actcgaaaat caccaccaac aatggctgga atcgtgtggt
    43621 cattggcccg gtgaaaggca aagagaacgc agacagcacc ctcaatcggt tgaagatggc
    43681 gggtcataca aactgcattc ggctcgccgc cgggggttga aaccctcaaa atccccccca
    43741 tctataattg cattatgccc cgtacttttg tacggggttt gtactctgta ttcgtaacca
    43801 aggggtcagc tcgtgacaac tatagtaagc gtacgccgta acggccatgt ggtcatcgct
    43861 ggtgatggtc aggccacgtt gggcaatacc gtaatgaaag gcaacgtgaa aaaggtccgc
    43921 cgtctgtaca acgacaaagt catcgcgggc tttgcgggcg gtactgcgga tgcttttacg
    43981 ctgttcgaac tgtttgaacg taaactggaa atgcatcagg gccatctggt caaagccgcc
    44041 gttgagctgg cgaaagactg gcgtaccgat cgcatgctgc gcaaacttga agcactgctg
    44101 gcagtcgcgg atgaaactgc atcgcttatc atcaccggta acggtgacgt ggtgcagcca
    44161 gaaaacgatc ttattgctat cggctccggc ggcccttacg cccaggctgc ggcgcgtgca
    44221 ctgttagaaa atactgaact gagtgctcgt gaaattgctg aaaaggcgtt ggatattgca
    44281 ggcgacattt gcatctatac caaccatttc cacaccatcg aagaattaag ctacaaagcg
    44341 taaggatctc ccatgtctga aatgacccca cgcgaaatcg tcagcgaact ggataagcac
    44401 atcatcggcc aggacaacgc caagcgttct gtggcgattg ctctgcgtaa ccgctggcgt
    44461 cgcatgcagc tcaacgaaga gctgcgccat gaagtgaccc cgaaaaatat cctgatgatc
    44521 ggcccgaccg gtgtcggtaa aactgaaatc gcccgtcgtc tggctaagct ggcgaatgcg
    44581 ccgttcatca aagttgaagc gaccaaattc accgaagtgg gctacgtcgg taaggaagtg
    44641 gattctatta ttcgcgatct gaccgatgcc gccgtgaaaa tggtacgcgt ccaggctatc
    44701 gagaaaaacc gttatcgcgc tgaagaactg gcagaagaac gtattctcga cgtgctgatc
    44761 ccacctgcta aaaacaactg gggacagacc gaacagcagc aggaaccgtc cgctgctcgt
    44821 caggcattcc gcaaaaaact gcgtgaaggc cagcttgatg acaaagaaat cgagatcgat
    44881 cttgccgcag caccgatggg cgttgaaatt atggctcctc caggcatgga agagatgacc
    44941 agccagctgc agtccatgtt ccagaacctg ggcggccaga agcaaaaagc gcgtaagctg
    45001 aaaatcaaag acgccatgaa gctgctgatt gaagaagaag cggcgaaact ggtgaacccg
    45061 gaagagctga agcaagacgc tatcgacgct gttgagcagc acgggatcgt gtttatcgac
    45121 gaaatcgaca aaatctgtaa gcgcggcgag tcttccggtc cggatgtttc tcgtgaaggc
    45181 gttcagcgtg acctgctgcc gctggtagaa ggttgcaccg tttccaccaa acacgggatg
    45241 gtcaaaactg accacattct gtttatcgct tctggcgcgt tccagattgc gaaaccgtct
    45301 gacctgatcc cggaactgca aggtcgtctg ccaatccgcg ttgaactgca ggcgctgacc
    45361 accagcgact tcgagcgtat tctgaccgag ccgaatgcct ctatcaccgt gcagtacaaa
    45421 gcactgatgg cgactgaagg cgtaaatatc gagtttaccg actccggtat taagcgcatc
    45481 gcggaagcgg catggcaggt gaacgaatct accgaaaaca tcggtgctcg tcgtttacac
    45541 actgttctgg agcgtttaat ggaagagatt tcctacgacg ccagcgattt aagcggtcaa
    45601 aatatcacta ttgacgcaga ttatgtgagc aaacatctgg atgcgttggt ggcagatgaa
    45661 gatctgagcc gttttatcct ataatcgcgt tcaatcattt tcatcattgt ttgatggggc
    45721 tgaaaggccc catttttatt ggcgcgtatt atgactgaac aacaaattag ccgaactcag
    45781 gcgtggctgg aaagtttacg acctaaaacc ctccccctcg cctttgctgc aattatcgtc
    45841 gggacggcgc tggcatggtg gcaaggtcac ttcgatccgc tggtcgccct gctggcacta
    45901 attaccgccg ggctattaca gatcctttct aacctcgcca atgattacgg cgatgcggta
    45961 aaaggcagcg ataaacctga ccgcattggg ccgctacgcg gcatgcaaaa aggggtcatt
    46021 acccagcaag agatgaaacg ggcgctcatt attaccgtcg tgctcatctg tctctccggg
    46081 ctggcactgg ttgcagtggc atgccatacg ctggccgatt ttgtcggttt cctgattctt
    46141 ggcgggttgt cgatcattgc cgctatcacc tacaccgtgg gcaatcgtcc ttatggttat
    46201 atcggtctgg gtgatatttc cgtactggtt ttctttggct ggttgagtgt catggggagc
    46261 tggtatttac aggctcatac attgattccg gcactgatcc ttccggcgac cgcatgcggc
    46321 ctgctggcaa cggcagtact gaatattaat aacctgcgtg atatcaatag cgaccgcgaa
    46381 aatggcaaaa acacgctggt ggtgcgctta ggtgaagtga acgcgcgtcg ttatcatgcc
    46441 tgcctgctga tgggctcgct ggtgtgtctg gcgctgttta atctcttttc gctgcatagc
    46501 ctgtggggct ggctgttcct gctggcggca ccattactgg tgaagcaagc ccgttatgtg
    46561 atgcgggaaa tggacccggt ggcgatgcga ccaatgctgg aacgtactgt caagggagcg
    46621 ttactgacta acctgctgtt tgttttaggg atattcctaa gccagtgggc agcataactg
    46681 acaaatatca attaacaatt gatgattttg ccaacagccc acatagcgcg atatactgaa
    46741 aattctcgca gcaactgaat gttaagccta tgaaatacga tacttccgag ctttgtgaca
    46801 tctatcaaga agatgttaac gtcgtggaac cgctgttctc caactttggc ggacgggcgt
    46861 cgtttggcgg acaaataatc acggtaaaat gtttcgagga caacgggttg ctgtacgatc
    46921 tgctcgaaca gaatggccgt ggtcgtgttc ttgtcgtcga tggcggtggt tctgttcgtc
    46981 gcgcactggt cgatgctgaa ctggcgcgtc tggcagtaca aaatgaatgg gaaggtctgg
    47041 tcatttacgg cgcggtgcgt caggtagatg acctggaaga gttggatatc ggcatccagg
    47101 cgatggcggc aattccggtt ggtgccgctg gcgaaggcat tggcgaaagc gatgtccgcg
    47161 tcaattttgg cggtgtcacc ttcttctccg gcgaccatct ttatgccgac aataccggga
    47221 ttattctttc tgaagatccg ctggatattg aatgatagaa aaggcaccgc aaggtgcctt
    47281 ttttctgcgt tacctgttgg cctacacagt aaagaaatta cgcggaagat gaagcgtaat
    47341 cagacctctt ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc gttctgctgt
    47401 tctttcagat ggttgttctc acgctccagc tcttcgcgct gatgctgggc attttgaact
    47461 tcctgcgaca gtgagttgtt tttttctttc agctcttcga tttccatctg caacagagtg
    47521 atggtatcaa tcgcctgctg tacttttgct tccagtttct caaacacttc taatgacatt
    47581 gtcatacctc tcctgaattg caaggcgttg atggataaaa aatcctcgtc ccgattaccg
    47641 gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca
    47701 tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg
    47761 gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa
    47821 acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgaa
    47881 gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca
    47941 tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca
    48001 ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg
    48061 taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc
    48121 gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta
    48181 cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct
    48241 gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc
    48301 tttctgcgct gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca
    48361 gactcatcac attgttcgcg gcagcgttga aagtattgat ctggctggca ctttctctac
    48421 ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc
    48481 tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc
    48541 tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt
    48601 gacaggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctggctggc
    48661 gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct
    48721 tttcggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg
    48781 ccatttgcct tgcgatatct gtgttgtgga agaaaaggaa accacaactc cttcagaaca
    48841 aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa aaatatatcg
    48901 ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata
    48961 tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt tgggtagaac
    49021 acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag
    49081 ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta
    49141 ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc
    49201 gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat atccgcagca
    49261 ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc
    49321 atgtggaagg ctctcgcgag cgtgcacgtc gtggtgaatt gctgtttggt acggttgata
    49381 cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct
    49441 ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc
    49501 tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta tacggtcaga
    49561 ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc
    49621 aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca
    49681 ctggctgctt tatgctgatg aacactggcg agaaagcggt gaaatcagaa aacggcctgc
    49741 tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa ggtgcggtgt
    49801 ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt aacgacgcct
    49861 acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg
    49921 catttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc
    49981 tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc
    50041 agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc
    50101 gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtcc gatattctcg
    50161 gcacccgcgt tgagcgcccg gaagttcgcg aagtgactgc attgggtgcg gcctatcttg
    50221 ctggtctggc ggtcggtttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg
    50281 aacgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga
    50341 aaaaagcggt caaacgcgcg atggcgtggg aagaacacga cgagtaatgt aaatgccgga
    50401 tgaagcgttt atgccgcatc cggtagtcct gaaaccgtgc gggggcaacc ccgcacacat
    50461 caataatccc acccttcccc tgtgctacac ttcgcgccat tccttactgc ttagagtttg
    50521 ctatgagacg agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg gcgctggctg
    50581 gctacaaatg gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg gtaaacgcca
    50641 tgcgtattat gctcaaccag gtcaacattg acggcaccat cgtcattggt gaaggtgaaa
    50701 tcgacgaagc accgatgctc tacattggtg aaaaagtcgg tactggtcgc ggcgacgcgg
    50761 tagatattgc tgttgatccg attgaaggca cgcgcatgac ggcgatgggc caggctaacg
    50821 cgctggcggt gctggcagta ggcgataaag gctgtttcct caacgcgcca gatatgtata
    50881 tggagaagct gatcgtcgga ccgggagcca aaggcaccat tgatctgaac ctgccgctgg
    50941 cggataacct gcgcaatgtc gcggcggcgc tcggcaaacc gttgagcgaa ctgacggtaa
    51001 cgattctggc taaaccacgc cacgatgccg ttatcgctga aatgcagcaa ctcggcgtac
    51061 gcgtatttgc tattccggat ggcgacgttg cggcctcaat tctcacctgt atgccagaca
    51121 gcgaagttga cgtgctgtac ggaattggtg gcgcgccgga aggcgtggtt tctgcggcgg
    51181 tgatccgcgc attagatggc gacatgaacg gtcgtctgct ggcgcgtcat gacgtcaaag
    51241 gcgacaatga agagaatcgt cgcattggcg agcaggagct ggcacgctgc aaagcgatgg
    51301 gcatcgaagc cggtaaagta ttgcgtctgg gcgatatggc gcgcagcgat aacgtcatct
    51361 tctctgccac cggtattacc aaaggcgatc tgctggaagg cattagccgc aaaggcaata
    51421 tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc cgcattcagt
    51481 ccatccacta tctggatcgc aaagacccag aaatgcaggt gcacattctc tgagtgattt
    51541 gatcgattga gccttccagt ccttcgggac tggaattttt ttgttcggag aacgaagata
    51601 aggcaagtca atcaaaacag gagaaaaaca tggctgattg ggtaacaggc aaagtcacta
    51661 aagtgcagaa ctggaccgac gccctgttta gtctcaccgt tcacgccccc gtgcttccgt
    51721 ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc gtccagcgcg
    51781 cctactccta tgttaactcg cccgataatc ccgatctgga gttttacctg gtcaccgtcc
    51841 ccgatggcaa attaagccca cgattggcgg cactgaaacc aggcgatgaa gtgcaggtgg
    51901 ttagcgaagc agctggcttc tttgttctgg atgaagtacc agattgcgaa acgctatgga
    51961 tgctggcaac cggtacagcg attggccctt atttatcgat tctgcaacta ggtaaagatt
    52021 tagatcgctt caaaaatctg gtcctggttc acgccgcacg ttatgccgcc gacttaagct
    52081 atttgccact gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc attcagacgg
    52141 tggtcagtcg ggaaacggca gcggggtcgc tcaccggacg gataccggca ttaattgaaa
    52201 gtggtgaact ggaaagcgcg attggcctgc cgatgaataa agaaaccagc catgtgatgc
    52261 tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt gctgaaagag acccgggaga
    52321 tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc ggagcattac tggtaagcgg
    52381 ttacttatcg ataaacggca cgatgagcaa atccgcactc aacttattga tcatcccgcg
    52441 atataccggc atcaaacggt tgataaatga gtggtgatga ccacagacaa ggaggtcgca
    52501 ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt caatacgtag
    52561 ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat ccgacttatt
    52621 cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat acaactcgct
    52681 taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg cgagctccag
    52741 ggctttattc accagtaagg catcttcttc attcccggaa attgccacgc caatgtgttt
    52801 ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg cagtgataac
    52861 tcaaaaataa tcatctcggc ctggcatgag aaggtgaagg ccgcatcaag ctcaacttgc
    52921 ccctccagat ccttaatctt ataggccacg ctggcccctt catctgcaga ggatgcagcg
    52981 gcggcaagct ctttcagttt ggtcataaag gactctgctt cttcgcgggt ggcaaaaaag
    53041 cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc aatcgcacat
    53101 cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat attcgcctct
    53161 ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa gtaatgatct
    53221 tatggattac catttttttt gatattgctt aaggtgaaga gttcagaagg attttccggg
    53281 tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa aatagcagta agagatatat
    53341 taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctgag tatctttacc
    53401 atatttattt tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca tcatcaccag
    53461 aatcaacgtc gggacaaaac gccccaccgc ccattgccag acacccggta aaatcgccca
    53521 gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc cggaacgccc
    53581 gcgatcatgc aagcgcttaa cagttactac cgctgttggc caaagcaagc acacaaggca
    53641 aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa gcaccaccat
    53701 gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac gccctttaaa
    53761 tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt ttccaggatt
    53821 ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat aacaaaaggt
    53881 actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat taaccgttac
    53941 aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc tgctggctgg
    54001 agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca gccagaatcc
    54061 tagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag ccaatctcac
    54121 tcgtgctgca agtaaaatta acgagaactt gtatgcttct acagcgctgg agcgcggtac
    54181 cttaaaaatc aaaagcattc aaatgacctg gctacccatc caggggccag agcaaaaagc
    54241 agcgaaagcg aaagctcagg aatacatggc agcagtgatc cgcacactca ccccattaat
    54301 gaccaaaaca caaagccaga aaaaactgca gtcgctgcta acggcgggga aaaacaaacg
    54361 ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaacg gcgaaaaggg
    54421 gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg aaggtttgaa
    54481 taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc acagacctgc
    54541 tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat taaaatgcga
    54601 catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt tcacgagctg
    54661 gtttctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc aatcgcacca
    54721 ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag gcagccacat catgctgggt
    54781 gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacttc tgccgctatg
    54841 ctgaaagaca tcggcgctca gtacatcatc atcggccact ctgaacgtcg tacttaccac
    54901 aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca gggcctgact
    54961 ccggttctgt gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac tgaagaagtt
    55021 tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg ctgcggcatt cgaaggtgcg
    55081 gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac tccggctcag
    55141 gcacaggctg ttcacaaatt catccgtgac cacatcgcta aagttgacgc taacatcgct
    55201 gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc agaactgttt
    55261 gctcagccag atatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc tgacgctttc
    55321 gcagtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca ggtgccggat
    55381 ttcatatccg gcacttactt tccttaactc ttcgccttaa cgcaaaatct cacactgatg
    55441 atcctgaatt tcctcggctg aagcacggtt aagcgtcagt agatttcgtt gtgtcgccag
    55501 caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc ccatatgttc
    55561 ccgcgcctca ggtacttctt ctgccagcat cataaatggg ctgcgttgta ccagttcgct
    55621 ttccgttacc cgacgcgcca ggtattcatg cccgcgcaaa ccacctggca gtggcaacca
    55681 gcggctgctg atgttcgcca gattgttatc cagttgttcg cgcacatcag gacgaataca
    55741 agagatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga tcgccaaaga
    55801 aaccgcgcga tcgggaaccg gctggccgta ttttttgctc ataaaatcac gcgcctgcca
    55861 ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac aaaggacttt cagtaccgtt
    55921 aatacgatac gttggtatca acagatattg cagtggacca ttaagatctt ttaaaaccac
    55981 gtatccggca ttgggtttga cttccgcaca tggcgaagga ttttgatttt gctgctgatt
    56041 gggcaaacat tcctcaagga caatcttacg taatgtatcc gactcttcac cggttaattt
    56101 ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa aaagaagacc
    56161 cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac gcaaaatcat
    56221 gacaaataaa aaacccggtc acgagaaacc accgggtttg ataattatcc tggggaaatc
    56281 agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa atgctctttt tgcgctttcg
    56341 tccagccgcc gaactcttca tcaatggtga ataacttcag ctttggaaac gcattttcgt
    56401 actttttcgc cacctcagcg tcgcgcggac ggtagtagtt tttcgcggca atttcctgac
    56461 cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg ccttttttct
    56521 cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca ctcggcgtga
    56581 cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttcg ttttcccagg
    56641 cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt agtggagccg cgcgcgccag
    56701 aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct tttgcctgat
    56761 cgttgttgtt gtgatgcagc gcgtagcccc aggcagccaa gtagttccag cgtgcgccac
    56821 cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga tcgttccagt
    56881 catggatctg cttcggattg cccttacgca ccaggaaaac aatggtggaa gtgtacggtg
    56941 cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccgcgt tccgcaattg
    57001 cgtccacgtc ataggccaga gccagcgtga caacatcagc ttcaataccg ttgattaccg
    57061 acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat caccacgtta tcaccagttt
    57121 gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg cgcgttggat
    57181 catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc gccagcaaaa
    57241 atgttaaccc tacgccccac ttgttcatcg cccgactctc ttatgttgtg ttgtgatgag
    57301 caaagcgtgc cagaaggtta accaaacatt aaagaataaa aaaagattgg ctataacttg
    57361 cgggcatatg ttgagggatt aaaaaggcgg aagaaatccg cctcatattg ctgacaaagt
    57421 gcgctttgtc catgccggat gcggcgcgaa cgccttatcc ggcctacaaa agtttgcaaa
    57481 ttcaataaat tgcagaattc atgtaggcct gataagcgaa gcgcatcagg catttttgct
    57541 tctgtcatcg gtttcaggct aaaggaatct gcctttttcc gaaatcatta atacagtttt
    57601 ttcgcgcagt ccagccagtc acctttgaac ggacgcttca tgttttcgat agcgtcgatg
    57661 atgtcgtggt gaaccagctg ttcgttctgg atacctacac aacgaccgcc gtaacctgcc
    57721 agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc gtaaggcacc
    57781 ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg accggtttct
    57841 ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat cgccacgatc
    57901 gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc ttcacggctg
    57961 aattcaactt ccggaaccac aacgaattca cagccaccgg caatggccgc agccaacgtc
    58021 agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg agaagaagag
    58081 gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa gaaaccgata
    58141 gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat gcacgggaag
    58201 cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgccgat aaccaccagc
    58261 gcgtcgatac cacgtttttt caggttttcg atagccacgg cgcggatgtt ctcgtcgcgg
    58321 aattccggga aacgcgcaga accgaggaac gtaccgccac ggttgatcat gtcagacacg
    58381 ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc gtcataaatg
    58441 cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat tgcggcgttc
    58501 atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat gactacctct
    58561 gaactttgga atgcaaaata aaatctgttg ccggaagtct tcttgcacat cgaagtgatc
    58621 caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag gtcaggccaa
    58681 atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga ttatgaaagc
    58741 atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat aattacatcc
    58801 gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat atgtgcctga
    58861 accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt cggccctgac
    58921 tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac gatatcaata
    58981 atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac cgcctcatat
    59041 cccatgcgta acgcgctata taaaatatag atgccgattc ccaatgcaaa cagagcatcg
    59101 gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc gttcatcata
    59161 acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt ccggcgcacc
    59221 acccaacgct gaaacgagac aaggataatc gtacaaatta gcgccacaat tgtcacgata
    59281 accccgacgc ctggatctgt catcggtgtt ggagatatca gatgttgaat acccgtcaaa
    59341 aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc gagggactca
    59401 gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata tcgcaccacc
    59461 agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc ggcgagaata
    59521 ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcaa cgaagccatc
    59581 gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt cataaatact
    59641 cccgctatca actgacgcta gtataacgga agcaaatcat ctgcaatgca ttaagcagca
    59701 ggcaaattga ggataaaaaa aacccccaca tcatgtgggg gaagacaggg atggtgtcta
    59761 tggcaaggaa aacagggttt actactggga acgtgagttg ctactactca atagcttcaa
    59821 cgatgaactt ttttgccatt gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt
    59881 ctcgtttaaa accgcttgct gctccggcgt taacaggcga tacatttggt tgcggacttt
    59941 tgccatctca acctgacgag caatttgctc attcgccatt ttttctgcct gtgcgcgcac
    60001 agcgttttca tcaaaatttt ctgaggtgac aaggcgatgc attgtctcca gttcgctaac
    60061 attaacagga ggctgttcgt gccgggcctg ttgcataaga tctcgcatct gctgacgctg
    60121 atgttcggtt aaacttatgc cgtcgaacat atggctctgc gtactgcgct gcgtaagttc
    60181 ttcacccgga tgccagttat cgcctgaacc gacttcagca gcgtggctta atgaactgac
    60241 tgccagcgtt gaggccatga cggcagcggt aactatgcgc atcatttgct cccaaaatct
    60301 ttctgtcgcg attcaacgat agagagttta cgattcaggc tgcaaacatg cgtcaggggg
    60361 tgtaaaacaa cgtaaagtca tggattagcg acgtctgatg acgtaatttc tgcctcggag
    60421 gtatttaaac aatgaataaa atcctgttag ttgatgatga ccgagagctg acttccctat
    60481 taaaggagct gctcgagatg gaaggcttca acgtgattgt tgcccacgat ggggaacagg
    60541 cgcttgatct tctggacgac agcattgatt tacttttgct tgacgtaatg atgccgaaga
    60601 aaaatggtat cgacacatta aaagcacttc gccagacaca ccagacgcct gtcattatgt
    60661 tgacggcgcg cggcagtgaa cttgatcgcg ttctcggcct tgagctgggc gcagatgact
    60721 atctcccgaa accgtttaat gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc
    60781 gttcgcactg gagcgagcaa cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg
    60841 atgccttagt gctgaatcca ggccgtcagg aagccagctt cgacgggcaa acgctggagt
    60901 taaccggtac tgagtttacc ctgctctatt tgctggcaca gcatctgggt caggtggttt
    60961 cccgtgaaca tttaagccag gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta
    61021 ttgatatgca catttccaac ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt
    61081 ggtttaaaac cttgcgtggt cgcggctatc tgatggtttc tgcttcatga taggcagctt
    61141 aaccgcgcgc atcttcgcca tcttctggct gacgctggcg ctggtgttga tgttggtttt
    61201 gatgttaccc aagctcgatt cacgccagat gaccgagctt ctggatagcg aacagcgtca
    61261 ggggctgatg attgagcagc atgtcgaagc ggagctggcg aacgatccgc ccaacgattt
    61321 aatgtggtgg cggcgtctgt tccgggcgat tgataagtgg gcaccgccag gacagcgttt
    61381 gttattggtg accaccgaag gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat
    61441 tcgtaacttt attggtcagg ccgataacgc cgatcatccg cagaagaaaa agtatggccg
    61501 cgtggaactg gtcggtccgt tctccgtgcg tgatggcgaa gataattacc aactttatct
    61561 gattcgtccg gccagcagtt ctcaatccga tttcattaac ttactgtttg accgcccgct
    61621 attactgctg attgtcacca tgttggtcag tacgccgctg ctgttgtggt tggcctggag
    61681 tctggcaaaa ccggcgcgta agctgaaaaa cgctgccgat gaagttgccc agggaaactt
    61741 acgccagcac ccggaactgg aagcggggcc acaggaattc cttgccgcag gtgccagttt
    61801 taaccagatg gtcaccgcgc tggagcgcat gatgacctct cagcagcgtc tgctttctga
    61861 tatctctcac gagctgcgca ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg
    61921 ccgtcgtagc ggtgaaagca aggaactcga gcgtattgaa accgaagcgc aacgtctgga
    61981 cagcatgatc aacgatctgt tggtgatgtc acgtaatcag caaaaaaacg cgctggttag
    62041 cgaaaccatc aaagccaacc agttgtggag tgaagtgctg gataacgcgg cgttcgaagc
    62101 cgagcaaatg ggcaagtcgt tgacagttaa cttcccgcct gggccgtggc cgctgtacgg
    62161 caatccgaac gccctggaaa gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc
    62221 ccatacgaag attgaagtgg gctttgcggt agataaagac ggtatcacca ttacggtgga
    62281 cgacgatggt cctggcgtta gcccggaaga tcgcgaacag attttccgtc cgttctatcg
    62341 gaccgatgaa gcacgcgatc gtgaatctgg cggtacagga ttggggctgg cgattgttga
    62401 aaccgccatt cagcagcatc gtggctgggt gaaggcagaa gacagcccgc tgggcggttt
    62461 acggctggtg atttggttgc cgctgtataa gcggagttaa actccgcatt tgtaggcagg
    62521 ataaggcgtt tacgccgcat ccggcatttg agcaggatgc ctgatgcgac gctgatagcg
    62581 tcttatcagg cctacactcc gggggttatt ttccccacaa ccggcgggaa aaatcttcga
    62641 tcttgccgct cagtcggcgc ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg
    62701 cggagagtaa acggtgatat tgatcgtcat caaacggcat atgccatgcg atggcggcag
    62761 cctcctgcac ggtcacatcg ctgacacggg aaaccaactc cagcggcgcg tccgcagaca
    62821 ctttccctgg cgctatcacg ctatacaacc accccacctt acctgtgttt tgcatcaact
    62881 gcgctatatc gctgatatca aaatggtaat tgagtttata gcaaggcgag cgcggctggc
    62941 tgacctgaat taatgcctct ccccagcgga aaatatcgcc catataaaca ttactttccg
    63001 tcaggccgtc ggttgagagg ttttcaccaa acgcaggcgc aacaaacaac tccgcctgtt
    63061 ccggaaattc ccgcgcccag tagagataat gctcacgagg ataatgacac agcgctctgt
    63121 ctggcccgcc gtgaaccttt ttctccgcct gctcgtcacc ttccagcccc agctccgtca
    63181 gcatcaactc accatcaacc tggattttgg caattgcgct gggtttgcct tcgggataag
    63241 cctgaatctt gcctgtgtat acatcaaccg gatatcgcat ctgttccctc tccacgagaa
    63301 atttccccat tagaccatat ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc
    63361 aggcaggaac tcaatagcaa accggacgaa cgttagttca tccggctgtg gggattaagg
    63421 aagcaggttt atttcaccgt acctaaaatt tcgattttcg ctgctctggc tttgacttta
    63481 cgtgaccaga tagaggtaag aattggcacc agaatcgaag tcacaatgac cgccgtcgct
    63541 accagcgaag ttgctgccgg agccattggt ttaaacgcag gcaccatttc tgcaatcagc
    63601 acaggtgtcg ctaccgcggc ccctgcggaa ctggaagcgg caattccggc agtgccatcg
    63661 ccaccgccaa tcaatttatc ggcgataatc aacggaatac cggtcacgat aattactgcc
    63721 acacccaaca ggatccccag caaaccagtc tgggcaatca cagtcaaatc aatggtattg
    63781 cccagcgcga aggcaaagaa tggaatcagc gtttgcaccg ctttgctgaa aaattctcgt
    63841 aattcagggt caaggttccc cagggcaaag cccaccagga acggtaatac tgcgccgacg
    63901 aaaacatgcg gttcaaacga ggcaatccct gcagtgccca gaataatcat cgtcatgagc
    63961 ggcccggact ccaacgacat caacacaaat gccccagctt cttcttttgt gccgtactgc
    64021 tgcatgatgg aagcgtaaag tccgccattg gtcatatcca tcgccgccac cagcgccagc
    64081 gttgaaagtc cggcaaagaa tccaacttca acaccatgtt ccggaataat gcgcgaggca
    64141 attgccgcaa ccacccacgc gacggcaatt tttgttacca ccagcgtacc ggatttacgc
    64201 aataccgttc ccgtcgcgct taattttatt gacgccccca tgcaaaaaaa ccacaccgcc
    64261 agaatgggca ccgtaccggt aatcatcccg ttggtgaatg atccaaaata tttccccgcc
    64321 cccggcgaga aggtgtggca cagtgcgcca aggaatagcg ggacgagcat catccccccc
    64381 gggattttct caatcgagcg ttttatctgc atttccatca cctgtcggca tatttttaag
    64441 ggctgatgcg taattcctca atggagcatc agtgttgatg gaaacgaaga tagacattca
    64501 tttaatcaat aaaagtgatc aaaaccacat caattgaaac actgttttat ttttatcgga
    64561 tcattgtttt tattttgaaa ttgatcacaa aaaaaccacc gccgttggcg atggttcatt
    64621 ttttaagctg atatgcggcc tatacgcctc attgcagcag gcggcaaatg attatttttt
    64681 cgccgcaaaa cgtgccgctg cttcgtccca gttcaccacg ttccagaact ctttaatgta
    64741 gtccggacgg cggttctgga atttcaggta gtaagcatgt tcccacacat ccaggcccag
    64801 aatcgggaag ccggaagcgc cagaaatagc ttcacccatc agcggagaat cctggttagc
    64861 agtagaaacc accgccagtt tatcgccttt cagcaccagc catgcccagc cggaaccaaa
    64921 gcgggaagct gccgcttttt caaattctgc tttgaagtta tcaacggagc cgaagtcacg
    64981 ttcgatagcc gctttcaggt caccctgcag ggtggtgcct tttttcagac ctttccagaa
    65041 caggctgtgg ttagcgtgac cgccagcgtt gttgcgcagt acggttttct tgtctgctgg
    65101 cagctggtcc agtttggtga tcagctcttc aaccggcagg ttggcaaatt ctggcaggct
    65161 ttccagcgcc gcgttggcgt tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc
    65221 catggtctgc ttatcgaagt gcggttccag ggcatcgtaa gcatacggca gggatggcag
    65281 ggtatagctc atattcatct ccagtattgt cgggcggccg attgttaatg ccgcgtaagc
    65341 agttggttca ttatagttaa ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc
    65401 gtaagggtat ggttttgcag gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc
    65461 cgtgattgcc agtaaatcga caacggcggc aacaggcgaa aggttaatcg acagcacgat
    65521 ttttacactc atctcgtcgg agatgtgaag cgacgaaaaa tgatgaggat aagaagatga
    65581 gtaacgcgat tacgatgggg atattttggc atttgatcgg cgcggccagt gcagcctgtt
    65641 tttacgctcc gttcaaaaaa gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg
    65701 ggattgtttc gtggattatt ctgccgtggg ccatcagcgc cctgttacta ccggatttct
    65761 gggcgtatta cagctcgttt agtctctcta cgctgctgcc tgtttttctg ttcggcgcta
    65821 tgtgggggat cggtaatatc aactatggcc tgaccatgcg ttatctcggc atgtcgatgg
    65881 gaattggcat cgccattggc attacgttga ttgtcggtac gctgatgacg ccaattatca
    65941 acggcaattt cgatgtgctg attaataccg aaggcggacg catgacgttg ctcggcgttc
    66001 tggtggcgct gattggcgta gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga
    66061 tgggcattaa agccgaagag ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg
    66121 gcattttctc tgccgggatg tcctttgcga tgaacgccgc aaaaccgatg catgaagccg
    66181 ctgccgcact aggcgtcgat ccactgtatg tcgctctgcc aagctatgtc atcatcatgg
    66241 gcggcggcgc gatcatcaac ctcggtttct gttttattcg tctggcaaaa gtgaaggatt
    66301 tgtcgctaaa agccgacttc tcgctggcaa aaccgctgat cactcacaat gtgttactct
    66361 cagcactggg cgggttgatg tggtatctgc aattcttttt ctatgcctgg ggccacgccc
    66421 gcattccggc gcaatatgac tacatcagtt ggatgctgca tatgagcttc tatgtattgt
    66481 gcggcggtat cgtcgggctg gtgctgaaag agtggaacaa tgcaggacgc cgtccggtaa
    66541 cggtgttgag cctcggttgt gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca
    66601 tggcgaatta atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt
    66661 cccgggtaaa caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat
    66721 cactgattaa ccggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgtc
    66781 gcagatattg attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacgcgct
    66841 cactgcacga tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg
    66901 ccagccgggt aatcagctta tccagcaacg tttcgctgga tgttggcggc aacgaatcac
    66961 tggtgtaacg atggcgattc agcaacatca ccaactgccc gaacagcaac tcagccattt
    67021 cgttagcaaa cggcacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg
    67081 cctgcgccat ccccacgcta cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc
    67141 ccggaatcgc tccctgccag tcaagattca gcttcagccg ctccgggcaa taaataatat
    67201 tctgcaaaac cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatataaa
    67261 agagatcg
//
LOCUS       DAJSKJ010000003        66231 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000003 DAJSKJ010000000
VERSION     DAJSKJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.003"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..473)
                     /gene="folK"
                     /locus_tag="ON345_000141"
     CDS             complement(<1..473)
                     /gene="folK"
                     /locus_tag="ON345_000141"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR3070264.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLS"
     gene            complement(470..1888)
                     /gene="pcnB"
                     /locus_tag="ON345_000142"
     CDS             complement(470..1888)
                     /gene="pcnB"
                     /locus_tag="ON345_000142"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR3070265.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1927..2853)
                     /gene="gluQRS"
                     /locus_tag="ON345_000143"
     CDS             complement(1927..2853)
                     /gene="gluQRS"
                     /locus_tag="ON345_000143"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR3070266.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2890..3345)
                     /gene="dksA"
                     /locus_tag="ON345_000144"
     CDS             complement(2890..3345)
                     /gene="dksA"
                     /locus_tag="ON345_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR3070267.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3523..4227)
                     /gene="sfsA"
                     /locus_tag="ON345_000145"
     CDS             complement(3523..4227)
                     /gene="sfsA"
                     /locus_tag="ON345_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR3070268.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4242..4772)
                     /gene="thpR"
                     /locus_tag="ON345_000146"
     CDS             complement(4242..4772)
                     /gene="thpR"
                     /locus_tag="ON345_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR3070269.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4846..7275
                     /gene="hrpB"
                     /locus_tag="ON345_000147"
     CDS             4846..7275
                     /gene="hrpB"
                     /locus_tag="ON345_000147"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR3070270.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGRASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSML
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7471..10005
                     /gene="mrcB"
                     /locus_tag="ON345_000148"
     CDS             7471..10005
                     /gene="mrcB"
                     /locus_tag="ON345_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR3070271.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10225..12468
                     /gene="fhuA"
                     /locus_tag="ON345_000149"
     CDS             10225..12468
                     /gene="fhuA"
                     /locus_tag="ON345_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR3070272.1"
                     /translation="MTRSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12519..13316
                     /gene="fhuC"
                     /locus_tag="ON345_000150"
     CDS             12519..13316
                     /gene="fhuC"
                     /locus_tag="ON345_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR3070273.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13316..14206
                     /gene="fhuD"
                     /locus_tag="ON345_000151"
     CDS             13316..14206
                     /gene="fhuD"
                     /locus_tag="ON345_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR3070274.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14203..16185
                     /gene="fhuB"
                     /locus_tag="ON345_000152"
     CDS             14203..16185
                     /gene="fhuB"
                     /locus_tag="ON345_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR3070275.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16343..17623)
                     /gene="hemL"
                     /locus_tag="ON345_000153"
     CDS             complement(16343..17623)
                     /gene="hemL"
                     /locus_tag="ON345_000153"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR3070276.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSIEDINNTIDAARRVFAKL"
     gene            17848..19269
                     /gene="clcA"
                     /locus_tag="ON345_000154"
     CDS             17848..19269
                     /gene="clcA"
                     /locus_tag="ON345_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR3070277.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19293..19358
                     /locus_tag="ON345_000155"
     CDS             19293..19358
                     /locus_tag="ON345_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070278.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19351..19695
                     /gene="erpA"
                     /locus_tag="ON345_000156"
     CDS             19351..19695
                     /gene="erpA"
                     /locus_tag="ON345_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR3070279.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19742..20365)
                     /locus_tag="ON345_000157"
     CDS             complement(19742..20365)
                     /locus_tag="ON345_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR3070280.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20403..21203)
                     /gene="btuF"
                     /locus_tag="ON345_000158"
     CDS             complement(20403..21203)
                     /gene="btuF"
                     /locus_tag="ON345_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR3070281.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASTRIILAAQQLCNALSQVD"
     gene            complement(21196..21894)
                     /gene="mtnN"
                     /locus_tag="ON345_000159"
     CDS             complement(21196..21894)
                     /gene="mtnN"
                     /locus_tag="ON345_000159"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR3070282.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21978..23495
                     /gene="dgt"
                     /locus_tag="ON345_000160"
     CDS             21978..23495
                     /gene="dgt"
                     /locus_tag="ON345_000160"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR3070283.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTGHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23625..25049
                     /gene="degP"
                     /locus_tag="ON345_000161"
     CDS             23625..25049
                     /gene="degP"
                     /locus_tag="ON345_000161"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR3070284.1"
                     /translation="MKKTTLALSVLALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25227..26357
                     /gene="cdaR"
                     /locus_tag="ON345_000162"
     CDS             25227..26357
                     /gene="cdaR"
                     /locus_tag="ON345_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR3070285.1"
                     /translation="MAQDIVARTMRIIDTNINVMDARGRIIGSGDRERIGELHEGALL
                     VLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLRKYGELVCMT
                     AEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDLNQPRVVAIV
                     EVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNSFGRWDAEDH
                     RKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQRMPESRCYF
                     YQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHNVQPLATSKA
                     LFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26446..26832)
                     /locus_tag="ON345_000163"
     CDS             complement(26446..26832)
                     /locus_tag="ON345_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR3070286.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26994..27806)
                     /gene="yaeI"
                     /locus_tag="ON345_000164"
     CDS             complement(26994..27806)
                     /gene="yaeI"
                     /locus_tag="ON345_000164"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR3070287.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRRVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27861..28685)
                     /gene="dapD"
                     /locus_tag="ON345_000165"
     CDS             complement(27861..28685)
                     /gene="dapD"
                     /locus_tag="ON345_000165"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR3070288.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28716..31388)
                     /gene="glnD"
                     /locus_tag="ON345_000166"
     CDS             complement(28716..31388)
                     /gene="glnD"
                     /locus_tag="ON345_000166"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR3070289.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDSFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31450..32244)
                     /gene="map"
                     /locus_tag="ON345_000167"
     CDS             complement(31450..32244)
                     /gene="map"
                     /locus_tag="ON345_000167"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR3070290.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEVE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32612..33337
                     /gene="rpsB"
                     /locus_tag="ON345_000168"
     CDS             32612..33337
                     /gene="rpsB"
                     /locus_tag="ON345_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR3070291.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33595..34446
                     /gene="tsf"
                     /locus_tag="ON345_000169"
     CDS             33595..34446
                     /gene="tsf"
                     /locus_tag="ON345_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR3070292.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34593..35318
                     /gene="pyrH"
                     /locus_tag="ON345_000170"
     CDS             34593..35318
                     /gene="pyrH"
                     /locus_tag="ON345_000170"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR3070293.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35610..36167
                     /gene="frr"
                     /locus_tag="ON345_000171"
     CDS             35610..36167
                     /gene="frr"
                     /locus_tag="ON345_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR3070294.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36259..37455
                     /gene="ispC"
                     /locus_tag="ON345_000172"
     CDS             36259..37455
                     /gene="ispC"
                     /locus_tag="ON345_000172"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR3070295.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANTREVARKE
                     VMRLAS"
     gene            37644..38402
                     /gene="ispU"
                     /locus_tag="ON345_000173"
                     /gene_synonym="uppS"
     CDS             37644..38402
                     /gene="ispU"
                     /locus_tag="ON345_000173"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR3070296.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38416..39273
                     /gene="cdsA"
                     /locus_tag="ON345_000174"
     CDS             38416..39273
                     /gene="cdsA"
                     /locus_tag="ON345_000174"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3070297.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRLQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39285..40637
                     /gene="rseP"
                     /locus_tag="ON345_000175"
     CDS             39285..40637
                     /gene="rseP"
                     /locus_tag="ON345_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR3070298.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVYERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40667..43099
                     /gene="bamA"
                     /locus_tag="ON345_000176"
     CDS             40667..43099
                     /gene="bamA"
                     /locus_tag="ON345_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR3070299.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43221..43706
                     /gene="skp"
                     /locus_tag="ON345_000177"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43221..43706
                     /gene="skp"
                     /locus_tag="ON345_000177"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR3070300.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43710..44735
                     /gene="lpxD"
                     /locus_tag="ON345_000178"
     CDS             43710..44735
                     /gene="lpxD"
                     /locus_tag="ON345_000178"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR3070301.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44840..45295
                     /gene="fabZ"
                     /locus_tag="ON345_000179"
     CDS             44840..45295
                     /gene="fabZ"
                     /locus_tag="ON345_000179"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3070302.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45299..46087
                     /gene="lpxA"
                     /locus_tag="ON345_000180"
     CDS             45299..46087
                     /gene="lpxA"
                     /locus_tag="ON345_000180"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR3070303.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            46087..47235
                     /gene="lpxB"
                     /locus_tag="ON345_000181"
     CDS             46087..47235
                     /gene="lpxB"
                     /locus_tag="ON345_000181"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR3070304.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47232..47828
                     /gene="rnhB"
                     /locus_tag="ON345_000182"
     CDS             47232..47828
                     /gene="rnhB"
                     /locus_tag="ON345_000182"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR3070305.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47865..51347
                     /gene="dnaE"
                     /locus_tag="ON345_000183"
     CDS             47865..51347
                     /gene="dnaE"
                     /locus_tag="ON345_000183"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR3070306.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDCFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51360..52319
                     /gene="accA"
                     /locus_tag="ON345_000184"
     CDS             51360..52319
                     /gene="accA"
                     /locus_tag="ON345_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR3070307.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52418..54559
                     /gene="ldcC"
                     /locus_tag="ON345_000185"
     CDS             52418..54559
                     /gene="ldcC"
                     /locus_tag="ON345_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR3070308.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54616..55005
                     /locus_tag="ON345_000186"
     CDS             54616..55005
                     /locus_tag="ON345_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3070309.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            55070..56368
                     /gene="tilS"
                     /locus_tag="ON345_000187"
     CDS             55070..56368
                     /gene="tilS"
                     /locus_tag="ON345_000187"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR3070310.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56417..56677)
                     /gene="rof"
                     /locus_tag="ON345_000188"
     CDS             complement(56417..56677)
                     /gene="rof"
                     /locus_tag="ON345_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR3070311.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56664..56864)
                     /locus_tag="ON345_000189"
     CDS             complement(56664..56864)
                     /locus_tag="ON345_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR3070312.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            57030..57575
                     /locus_tag="ON345_000190"
     CDS             57030..57575
                     /locus_tag="ON345_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR3070313.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLHNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAHIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57572..57994
                     /gene="arfB"
                     /locus_tag="ON345_000191"
     CDS             57572..57994
                     /gene="arfB"
                     /locus_tag="ON345_000191"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3070314.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            58008..58718
                     /gene="nlpE"
                     /locus_tag="ON345_000192"
                     /gene_synonym="cutF"
     CDS             58008..58718
                     /gene="nlpE"
                     /locus_tag="ON345_000192"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR3070315.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDELEMLDREGNPIESQFNYTLEPAQSSLPLTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58873..59696)
                     /locus_tag="ON345_000193"
                     /pseudo
     CDS             complement(58873..59696)
                     /locus_tag="ON345_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59750..61468)
                     /gene="proS"
                     /locus_tag="ON345_000194"
     CDS             complement(59750..61468)
                     /gene="proS"
                     /locus_tag="ON345_000194"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR3070316.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61580..62287)
                     /gene="tsaA"
                     /locus_tag="ON345_000195"
     CDS             complement(61580..62287)
                     /gene="tsaA"
                     /locus_tag="ON345_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR3070317.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62284..62688)
                     /gene="rcsF"
                     /locus_tag="ON345_000196"
     CDS             complement(62284..62688)
                     /gene="rcsF"
                     /locus_tag="ON345_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR3070318.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62806..63621)
                     /gene="metQ"
                     /locus_tag="ON345_000197"
     CDS             complement(62806..63621)
                     /gene="metQ"
                     /locus_tag="ON345_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR3070319.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63661..64314)
                     /gene="metI"
                     /locus_tag="ON345_000198"
     CDS             complement(63661..64314)
                     /gene="metI"
                     /locus_tag="ON345_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR3070320.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64307..65338)
                     /gene="metN"
                     /locus_tag="ON345_000199"
     CDS             complement(64307..65338)
                     /gene="metN"
                     /locus_tag="ON345_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR3070321.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65526..66101
                     /gene="gmhB"
                     /locus_tag="ON345_000200"
     CDS             65526..66101
                     /gene="gmhB"
                     /locus_tag="ON345_000200"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR3070322.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15606 a        16538 c        18266 g        15821 t
ORIGIN      
        1 ttgcttaatt tgtcaaatgc tcttgtatgt aagacttcac gcaacgtctc cccatcagga
       61 aacgccagct ccggtgcaat ctcaaacagc ggccacagca taaatccacg attcttcata
      121 tcgtagtgcg gaacggtcag gcgttcggta tttatcactt cattaccaaa cagcatgata
      181 tcgagatcca gcgtgcgtgg tccccagcgt tcagctttac gaacacgacc ctgttgcagt
      241 tctatgcgct gtgtatggtt gagtaactct tccggggcga gcgaagtttc aagcgccacg
      301 gcggcgttga ggtaatcggg ttgatcttgc ggccccagcg gtggggtgcg gtaaaacgaa
      361 gaaacggcaa gaatgtggct ttcagggata tcgcctaatg ctttcagggc agcattgacc
      421 tgctccagcg gagaggccag attgctgcct atggcaatat acgccactgt catgcggtac
      481 cctcacgacg cggtgcgcgt ttgcgtggac gacgagtacg acgacgcggt gacggttctt
      541 catccagctc gttaagcatc cctttttgat ctggtggtgc ggaaacctgg aactcacccc
      601 accatttcac cagacgctgc agttcagcat tacgttcaac ttcagctcgc aaggccaaca
      661 ggtcataagc cgcacggaac ttaggatgct ccagcagttt ccatgcgcgt ttaccctgac
      721 gacgggacat acgcaactgc aactgccaga tatcgcgggt taacgtcgtc agacgtttcg
      781 ggattgccag tgaacggcag gcttcgtcca gcacgtcgtt catcgccagc gcgaaagcgt
      841 cgtgataggt caggccgctt tcctgggcga tcttctgtgc cgtctccagc agtgggtacc
      901 agaacatggc ggcaaacaag aacgccgggt tcacgcgcat atcgttatgg atacgcgtat
      961 cggtattctt cagcacctgt tcaatgatcc gctccatcgg gctgtcgcca ttttccgtga
     1021 agtagcgggt aatggtcggg aacagcggct ggaacagatg atattcacac aacagcttat
     1081 aggtttcgta accgtagccc gcttgtagca gtttaagcga ttcttcaaac aggcgtgccg
     1141 gtgggatatc gttcagcagg gtagcgaggc gagggatcgg ttctgcggtt tccgggctga
     1201 tgcgcatacc caatttggcg gcaaaacgta ccgcgcgcag catacgtacc ggatcttcac
     1261 ggtagcgcgt ttccgggtta ccaatcagac ggataacgcc gtccttcaga tccttcatac
     1321 cgccaacgta atcacggacg gtaaaatccg ctacgctgta atagaggctg ttgatagtga
     1381 aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat gttgtcgcgc agcaacatgc
     1441 cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt accttcgtgg tgtccacgga
     1501 aggtcgcaac ttcgataatc tccgggccaa acattacatg agccagacgg aaacggcgac
     1561 ccaccaggcg gcagttacgg aacagtttgc gcacctgctc aggcgtggcg ttagtggtta
     1621 cgtcaaaatc tttcggcttt ttgccaagta acaggtcgcg cacgccgccg ccaaccagcc
     1681 aggcttcgta tcccgctttg ttgagcctgt acattacctt cagggcattt tcactgatat
     1741 ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt cacctgtgga cgggcgactg
     1801 cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg gcaaaaatta gcgactcggg
     1861 taaaaatagt acacctcggt agtgtcaaac atcattcagg acaaaaaaat agcggctaat
     1921 catagctcag catgacgcat ttgagaatgt tgaatttaca attgccgact cgggcacggc
     1981 ggtcagcgtc cagtttttga cggctgactg aaggatttgt tcgacgctga aatcctgcca
     2041 gtgtgcttct gcctgctgac ccagaaattg aagtgccgcg attagtaccg gacgtggatc
     2101 gcctttcggc aacgcaggcg catgattctg cttggaaagt ttagcgcctt gtggattaag
     2161 cgccagcggc agatgaatgt aatctggcac tttccagcca aaaagctggt acagcgagat
     2221 ttgccttact gttggttcaa tcagatcagc cccacgcact atttctgtaa cgccctggaa
     2281 atgatcatca accacaacag ccaggttgta ggcgaacaac ccatcacggc gatgaatgat
     2341 aaaatcttcc cgtgccagtt tttcgtcggc gtgaataatg ccgcgcagct ggtcagtaaa
     2401 ttgcgtgacc ggatgctgct ggcggatacg cactgcggcg ttgtctggtc catgatgcaa
     2461 cacccggcaa tgaccgtcgt aaataccgcc aatgctttga atacgcgcac gcgtacaggt
     2521 gcagtaataa cttagtcctt gttcatgtaa ccaggcgagt gcttcacgat aggcgtcgtg
     2581 acgttgcgat tgccagagaa catcgccgtc ccagtgcaga ccgtaatgtt ccagctggcg
     2641 caggatagtt tctgcggcac cgggaacttc acgaggcggg tcgatatctt ctatgcgtac
     2701 cagccagcga ccttgccggg cgcgagcctg caaatagctg ccgagcgcgg cgatcagaga
     2761 gccaaaatga agctcgccgg aaggagaggg ggcgaagcgg ccaatatact gtgtgtctgt
     2821 catctctttg aacaaaaaat aaggcgggag catttcccgc ctgtggtaaa cgtgatggaa
     2881 cggctgtaat tagccagcca tctgtttttc gcgaatttca gccagcgttt tgcagtcgat
     2941 gcacagatcg gctgtcgggc gcgcttccag acggcgaata ccaatttcaa caccgcagga
     3001 ttcgcagtag ccgaaatctt cgtcttccac ttttttcagc gtcttctcga tctttttgat
     3061 cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg aactcttctt cctgggctgc
     3121 acggtctacc gggtccggga agttggctgc ttcatcctgc atatgtgtaa cggtgcgatc
     3181 gacttcatcc ctgagttgat tacgccatgc ttccagaata cgacggaagt gcgccagctg
     3241 ggcttcattc atatactctt cgcccggctt ctcctgatat ggttccaccc cagcgatggc
     3301 gagaatactc agggacgatg ttttacggtt ttgcccttct tgcatgttgc ttctccttaa
     3361 cacgcactat cgatccccat gttcggggga aaaatgaggc cgctataaat agcagatgct
     3421 tttccggata gcaattatct aaacgtaaca cttgacaact gtgtgaggaa aagcgtattt
     3481 gcgcacgcga tcagaatgta aattaaccag ttacttactt tactacaatg taaccggcag
     3541 tgatttttta agagccatgc cttcagcaga aatttccgct ttgtaagcca gaatttctac
     3601 ccccctctgt tgagcttctg acaatagttg cgcgtatttc tcatcgatgt ggcgcgcggg
     3661 tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaag ataaccgcac gctggccttc
     3721 agccgctacg ctcatcaact cccgaaggtg tttctgacct cgttcagtga ccgcatcggg
     3781 aaaatatccc tgttcgttct ccgctaacgt aaccgatttc acttcaatat agcagtctgg
     3841 acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt tctgcgccgt attttacttc
     3901 gcttttcagc gagctatagc ctgacagttc tgaaattgat tcattaagga tagcctcttt
     3961 cgtcaacctg ttagcccaaa gcgtgttgac gcaaataaat gcgccgctct ggctttgagt
     4021 taattcccag gtgtgtgggt atttccgttt ggtgttgtct gaagtcgaat accagacggt
     4081 atcgccaggc gttgcacaac cggtcatcgc acccgtattc gggcagtgta gcgttaattc
     4141 gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg taacgctgaa ttagcgtcgc
     4201 gcgctgtaga gggggagaaa attccattgc gacaatcctt gttattgcgt tagcgcccag
     4261 cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg aggaggcgta aagggtgaac
     4321 tccgtcaccg catacgacca gttaaaacct ggcggcggga ttgtcaccgc ctcgctggcg
     4381 tcgcgcaata aggtaatatg tggatgaaac ggacgattgc tttgaaaaca accgctgcgg
     4441 gcagcctgtg aacggagcat attcgccagc tggattaagc cgcgtggcgg ctggcgcatc
     4501 cctaaccaca ccacacgcga acgcagccat tgtccggcgt catcaagcgt gagtgtgaaa
     4561 ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct tctctttctc ggcgctcact
     4621 tcgcctaaaa atgccagagt cagatgcaaa ttatcggcgg cgaccggacg tcccgcctca
     4681 ggtgggaagt gtgtggcgcg ccaatggata atctgttcgc ggatttctgc aggtaagtcg
     4741 atagcaaaga acagacgttg cggttcagac atgtgaggca ctcggttatg aattaccgcg
     4801 atgctacaat gtggcgcgaa gaatgttaac cctctggagc gttttgtgtc gtcgttgccc
     4861 gttgctgccg tcttacctga attacttacc gctctcgatt gtgcgccgca ggtattatta
     4921 agtgcgccga ccggggccgg gaaatcaacc tggctgccgc tgcaactgct ggcgcatccc
     4981 ggcattaacg ggaaaattat cctgctggag ccgcgtcgtc tggcggcgcg taacgtcgcg
     5041 caacggctgg cggagctgct taacgaaaag ccaggcgata ccgttggcta ccggatgcgt
     5101 gcgcaaaact gcgtcgggcc gaatacccgc ctggaagtgg ttaccgaagg cgtgctgacg
     5161 cgcatgatcc agcgtgaccc ggaactgagc ggtgttggac tggtgatcct tgatgaattt
     5221 catgagcgca gcttgcaggc ggatttggcg ttggcgctgt tactcgatgt gcaacaaggt
     5281 ctgcgtgatg accttaaact gctgattatg tcggctaccc tggacaacga ccgcttgcag
     5341 caaatgctgc cagaagcgcc tgtcgtcatc tcagaagggc gctcgtttcc ggttgaacgc
     5401 cgttatttac cgctgcccgc gcatcagcgt tttgacgatg ccgttgcggt agccaccgct
     5461 gaaatgctgc gtcaggaaag cggatcatta ctgttatttt tacctggcgt cggagaaatt
     5521 cagcgtgtgc aggaacaact ggcttcgcgc atcggcagtg atgtattgct ctgcccgctg
     5581 tatggcgcgt tgtcgctgaa cgatcagcga aaagcgatcc tcccggcacc gcaagggatg
     5641 cgcaaagtgg tgctggcgac caatattgct gaaaccagtt taaccattga aggtattcgt
     5701 ctggtggtgg attgtgccca ggagcgtgtg gcgcgttttg atccgcgcac ggggcttacg
     5761 cgactgatta ctcaacgcgt tagccaggca tccatgacgc agcgtgccgg gcgcgccggg
     5821 cgtctggagc cgggtatctg cctgcattta atcgccaagg aacaagcaga acgcgctgcc
     5881 gcgcaaagtg aaccggaaat cttacaaagc gatctttccg gtttgctgat ggaattactg
     5941 caatggggat gcagcgatcc ggcgcagatg agctggctgg atcaaccgcc aacggtgaat
     6001 ctactggccg cgaaacgcct gttacaaatg ctgggggcgc tggagggtga acggcttagt
     6061 gcgcaggggc aaaaaatggc agcgctgggt aacgatccgc gtttagcggc aatgctggtt
     6121 agcgcgaaga acgacgacga agctgctacc gcggcaaaaa ttgccgccat tctcgaagag
     6181 ccgccacgga tgggcaatag tgacctgggc gtggcgtttt cgcgcaatca accagcctgg
     6241 cagcaacgta gtcagcaact gttaaaacgc ttaaacgtac gtggcggtga ggcagacagt
     6301 tcgcttatcg cgccgctact tgccggggcg tttgccgatc gcattgctca tcgccgtggg
     6361 caagatggac gctatcaact ggcaaacggc atgggagcga tgctcgatgc cgacgacgcg
     6421 ctaagccgcc acgaatggtt gatcgcaccg ttattattgc agggccgcgc ctcgccggat
     6481 gcgcggattt tactggcgct accggtcgat attgatgaat tagtacaacg ctgtccgcag
     6541 ctggtgcagc aatctgacac cgtggaatgg gatgacgcgc aaggtacgct gaaagcctgg
     6601 cgtcggctac aaatcggtca gttgacggtg aaagtgcagc cgctggcgaa accctcggaa
     6661 gacgagttgc atcaggcgat gcttaatggc attcgtgata aaggtttaag catgctcaac
     6721 tggacggcgg aagcggaaca gctacgcttg cgtttgttat gcgccgcaaa gtggttgccg
     6781 gaatatgact ggccagcggt tgatgatgaa agtttattgg cagcgctgga aacgtggctg
     6841 ctgccacata tgactggcgt acattcacta cgcggcctga aatcactcga catttatcag
     6901 gcactacgcg gattacttga ttggggaatg cagcaacgtc tggatagtga attgcctgcg
     6961 cattacactg tgccgacggg aagccggatc gccattcgtt atcatgaaga taacccgccc
     7021 gcgctggcgg tgagaatgca agagatgttt ggcgaggcca ccaatccgac gatcgcccag
     7081 gggcgcgtgc cgctggtgct ggagttgctt tcacctgccc aaaggccatt acaaatcaca
     7141 cgagatttga gcgccttctg gaaaggagcg taccgtgagg tgcaaaaaga gatgaaaggg
     7201 cgttatccca aacatgtctg gccggacgac ccggcaaata ctgcaccgac gcgacggacg
     7261 aaaaagtatt cgtaaggttg gttttctccc tctccctgtg ggagagggcc ggggtgaggg
     7321 catcagcgcg cacgttcacc ctcaccctaa ccctctccct caagggagag gggaccgatc
     7381 gagcacaaat tttgagagat atcttcttct gtcttgtaac agaagaacag aaaatcgggc
     7441 ttttgcgcct gaatattgcg gagaaaaagc atggccggga atgaccgcga gccaattgga
     7501 cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa gccgtcgtcg ttacgaagat
     7561 gacgatgatt acgacgatta tgatgactat gaggatgaag aaccgatgcc gcgcaaaggt
     7621 aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg gctggctatg gctactgcta
     7681 aaactggcta tcgtttttgc cgtgctgatc gccatttacg gcgtttatct cgatcaaaaa
     7741 attcgtagcc gtattgatgg caaggtctgg caactgcctg cggcagttta tggccgaatg
     7801 gtcaatcttg agccagacat gaccatcagc aagaacgaga tggtgaagct gctggaggcg
     7861 acccagtatc gtcaggtgtc gaaaatgacc cgtcctggcg aatttaccgt gcaggccaac
     7921 agcattgaga tgattcgccg tccgtttgat ttcccggaca gtaaagaagg acaggtgcgc
     7981 gcgcgtctga cctttgatgg cgatcatctg gcgacgatcg tcaatatgga gaacaaccgt
     8041 cagttcggtt tcttccgtct tgatccgcgt ctgatcacca tgatctcttc gccaaacggt
     8101 gagcagcgtc tgtttgtgcc gcgcagtggt ttcccggatt tgctggtgga tactttgctg
     8161 gcgacagaag accgtcattt ttacgagcat gatggaatca gtctctactc aatcggacgt
     8221 gcggtgctgg caaacctgac cgccggacgc acggtacagg gtgcgagtac gctgacgcaa
     8281 cagctggtga aaaacctgtt cctctccagc gagcgttctt actggcgtaa agcgaacgaa
     8341 gcttacatgg cgctgatcat ggacgcgcgt tacagcaaag accgtattct tgagctgtat
     8401 atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa tccgcggctt cccgctggca
     8461 agcttgtatt actttggtcg cccggtagaa gagctaagcc tcgaccagca ggcgctgtta
     8521 gtcggtatgg tgaaaggggc gtccatctac aacccgtggc gtaacccaaa actggcgctg
     8581 gagcgacgta atctggtgct gcgtctgctg caacagcaac agattattga tcaagaactc
     8641 tatgacatgt tgagtgcccg tccgctgggg gttcagccgc gcggtggggt gatctctcct
     8701 cagccagcct ttatgcaact ggtgcgtcag gagctgcagg caaaactggg cgataaggta
     8761 aaagatctct ccggcgtgaa gatcttcact acctttgact cggtggccca ggacgcggca
     8821 gaaaaagccg ccgtggaagg cattccggca ctgaagaaac agcgtaagtt gagcgatctt
     8881 gaaactgcga ttgtggtcgt cgaccgcttt agtggtgaag ttcgtgcgat ggtcggaggt
     8941 tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg cgcgtcgttc gattggttcc
     9001 cttgcaaaac cagcgactta tctgacggct ttaagccagc cgaaaatcta tcgcctgaac
     9061 acgtggattg ccgatgcgcc aatcgcgctg cgtcagccga atggccaggt ctggtcaccg
     9121 cagaacgatg accgtcgtta tagcgaaagc ggcagagtga tgctggtgga tgcgttgacc
     9181 cgttcgatga acgtgccgac ggtaaatctg gggatggcgc tggggctgcc tgcagttacg
     9241 gatacctgga tcaaactggg cgtaccgaaa gatcagctgc atccggttcc ggcgatgctg
     9301 ctgggggcgt tgaacttaac gccaatcgaa gtggcgcagg cgttccagac catcgccagc
     9361 ggtggtaacc gtgcaccgct ttctgcactg cgttcggtaa tcgcggaaga tggcaaggtg
     9421 ctgtatcaga gcttcccgca ggcagaacgc gctgttccgg cgcaggcggc gtatctgaca
     9481 ctatggacca tgcagcaggt ggtacaacgt ggtacgggtc gtcagcttgg ggcgaaatac
     9541 ccgaacctgc atctggcagg gaaaacaggg actaccaaca ataacgtaga tacctggttt
     9601 gcaggcattg acggcagcac ggtgaccatc acctgggtag gccgtgataa caaccagccg
     9661 accaaactgt atggagccag cggggcaatg tcgatttatc agcgttatct ggctaaccag
     9721 acgccaacgc cgctgaatct tgttccgcca gaagatattg cagatatggg cgtggactac
     9781 gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc cggtctggac cagcgatccg
     9841 caatcgctgt gccagcagag cgagatgcag cagcagccgt caggcaatcc gtttgatcag
     9901 tcttctcagc cgcagcaaca gccgcaacag caacctgctc agcaagagca gaaagacagc
     9961 gacggtgtag ccggttggat caaggatatg tttggtagta attaacatct aagcgtgaaa
    10021 taccggatgg cgagttgcca tccggtaaaa taacatccca tctaagatat taaccctttc
    10081 ttttcatctg gttgtttatt aacccttcag gaacgctcag attgcgtacc gcttgcgaac
    10141 ccgccagcgt ttcgaatatt atcttatctt tataataatc attctcgttt acgttatcat
    10201 tcactttaca tcagagatat accaatgacg cgttccaaaa ctgctcagcc aaaacactca
    10261 ctgcgtaaaa tcgcagttgt agtagccaca gcggtaagcg gcatgtctgt ttatgcacag
    10321 gcagcggttg aaccgaaaga agacactatc accgttaccg ctgcacctgc gccgcaagaa
    10381 agcgcatggg ggcctgctgc aactattgcg gcgcgacagt ctgctaccgg cactaaaacc
    10441 gatacgccga ttcaaaaagt gccacagtct atttctgttg tgaccgccga agagatggcg
    10501 ctgcatcagc cgaagtcggt aaaagaagcg cttagctaca cgccgggtgt ctctgttggt
    10561 acgcgtggcg catccaacac ctatgaccac ctgatcattc gcggctttgc ggcagaaggc
    10621 caaagccaga ataactatct gaatggcctg aagttgcagg gcaacttcta taacgatgcg
    10681 gtcattgacc cgtatatgct ggaacgcgct gaaattatgc gtggcccggt ttccgtgctt
    10741 tacggtaaaa gcagtcctgg cggcctgttg aatatggtca gcaagcgtcc gaccaccgaa
    10801 ccgctgaaag aagttcagtt taaagccggt actgacagcc tgttccagac tggttttgac
    10861 tttagcgatt cgttggatga tgacggtgtt tactcttatc gcctgaccgg tcttgcgcgt
    10921 tctgccaatg cccagcagaa agggtcagaa gagcagcgtt atgctattgc accggcgttc
    10981 acctggcgtc cggatgataa aaccaatttt accttccttt cttacttcca gaacgagccg
    11041 gaaaccggtt attacggctg gttgccgaaa gagggaaccg ttgagccgct gccgaacggt
    11101 aagcgtctgc cgacagactt taatgaaggg gcgaagaaca acacctattc tcgtaatgag
    11161 aagatggtcg gctacagctt cgatcacgaa tttaacgaca cctttactgt gcgtcagaac
    11221 ctgcgctttg ctgaaaacaa aacctcgcaa aacagcgttt atggttacgg cgtctgctcc
    11281 gatccggcga atgcttacag caaacagtgt gcggcattag cgccagcgga taaaggccat
    11341 tatctggcac gtaaatacgt cgttgatgat gagaagctgc aaaacttctc cgttgatacc
    11401 cagttgcaga gcaagtttgc cactggcgat atcgaccaca ccctgctgac cggtgtcgac
    11461 tttatgcgta tgcgtaatga catcaacgcc tggtttggtt acgacgactc tgtgccactg
    11521 ctcaatctgt acaatccggt gaataccgat ttcgacttca atgccaaaga tccggcaaac
    11581 tccggccctt accgcattct gaataaacag aaacaaacgg gcgtttatgt tcaggatcag
    11641 gcgcagtggg ataaagtgct ggtcacccta ggcggtcgtt atgactgggc agatcaagaa
    11701 tctcttaacc gcgttgctgg gacgaccgat aaacgtgatg acaaacagtt tacctggcgt
    11761 ggtggtgtta actacctgtt tgataatggt gtaacacctt acttcagcta tagcgaatcg
    11821 tttgaacctt cttcgcaagt tgggaaggat ggtaatattt tcgcaccgtc taaaggtaag
    11881 cagtatgaag tcggcgtgaa atatgtaccg gaagatcgtc cgattgtagt tactggtgcc
    11941 gtgtataatc tcactaaaac caacaacctg atggcggacc ctgagggttc cttcttctcg
    12001 gttgaaggtg gcgagatccg cgcacgtggc gtagaaatcg aagcgaaagc ggcgctgtcg
    12061 gcgagtgtta acgtagtcgg ttcttatact tacaccgatg cggaatacac caccgatact
    12121 acctataaag gcaatacgcc tgcacaggtg ccaaaacaca tggcttcgct gtgggctgac
    12181 tataccttct ttgacggtcc gctttcaggt ctgacgctgg gcaccggtgg tcgttatact
    12241 ggctccagct atggtgatcc ggctaactcc tttaaagtgg gaagttatac ggtcgtggat
    12301 gcgttagtgc gttatgatct ggcgcgagtc ggcatggcgg gctccaacgt ggcgctgcat
    12361 gttaacaacc tgttcgatcg tgaatacgtc gccagctgct ttaacactta tggctgcttc
    12421 tggggcgcag aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt tctcttttgg
    12481 ggcacggatt tccgtgccca tttcacaagt tggctgttat gcaggaatac acgaatcatt
    12541 ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc acgcttttgc
    12601 atccgctgtc gttaaccttt cctgccggga aagtgaccgg tctgattggt cacaacggtt
    12661 ctggtaaatc cactctgctc aaaatgcttg gccgtcatca gcctccgtca gaaggggaga
    12721 ttcttcttga tgcccagccg ctggaaagct ggagcagcaa agcgtttgcc cgcaaagtgg
    12781 cttatttgcc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa ctggtggcga
    12841 ttggtcgtta cccgtggcat ggcgcgctgg ggcgctttgg tgcggcagat cgcgaaaagg
    12901 tcgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg gtcgatagtc
    12961 tctctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag gatagccgtt
    13021 gtctgttgct cgacgaaccg acttcggcgc tggatatcgc ccaccaggtt gatgtgctgg
    13081 cgctggtgca ccgtttaagt caggaacgtg gcctgacggt cattgccgtg ctgcacgata
    13141 tcaatatggc ggcacgctac tgtgattatc tggtcgccct gcgcggtggt gaaatgattg
    13201 ctcagggaac gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt tatggcatcc
    13261 cgatgggtat tttgccgcat ccggcgggtg ctgcacctgt gagttttgtt tattgatgag
    13321 cggcttacct cttatttcgc gccgtcgact gttaacggcg atggcgcttt ctccgttgtt
    13381 atggcagatg aataccgccc acgcggcggc tattgacccc aatcgtattg tggcgctgga
    13441 gtggttgccg gtggaattac tgctggcgct cggcatcgtg ccttacggcg tggcggatac
    13501 catcaactat cgcctgtggg tcagcgaacc accattgccg gactcagtga ttgacgtcgg
    13561 tttgcgtaca gaacctaacc ttgaactgct gaccgaaatg aaaccgtcgt ttatggtctg
    13621 gtcggcagga tatggccctt catcagaaat gctggcacgt attgcgccgg gtcgcggatt
    13681 taacttcagt gacggcaaac agccgttggc gatggcgcgt aaatcgctga cggaaatggc
    13741 agatttactt aacctgcaaa gcgcagcgga aacgcattta gcgcaatacg aagactttat
    13801 ccgcagcatg aaaccccgct ttgtgaagcg tggtgcacgc ccgttactgc tgacgacgct
    13861 tatcgatccg cgccatatgc tggtcttcgg tccaaacagc ttgttccagg aaattctcga
    13921 tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca gtaccgccgt
    13981 cagtatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg atcacgacaa
    14041 cagcaaagac atggatgcgc taatggcaac cccgctgtgg caggccatgc cgtttgtccg
    14101 cgccggacgc tttcagcgcg taccagcagt ctggttttat ggtgcgacac tctcggcaat
    14161 gcactttgtg cgcgttctgg ataacgccat cggaggtaaa gcgtgagtaa acgaattgcg
    14221 ctttttccgg cgttattgct ggcgctgtta gtgattgtcg ctacggcgct cacctggatg
    14281 aacttctcgc aggcgctgcc gcgtagccag tgggcgcagg ctgcctggtc gccagacatc
    14341 gatgtcatcg agcagatgat ttttcactac agcttgttgc cgcgtctggc gatttcgctg
    14401 ctggtgggcg cgggcctggg gctggtgggc gtgctgtttc agcaagtgct gcgtaacccg
    14461 ctggcggagc cgacgacgct tggcgttgct acaggcgcgc aactgggaat taccgtcacc
    14521 acactttggg cgatcccagg tgctatggcg agccagtttg ccgcgctggt gggcgcttgt
    14581 gtcgttggct taatcgtctt tggcgtcgcg tgggggaaac ggctgtcgcc ggtaacgctg
    14641 attctcgcgg ggctggtagt gagcctttat tgtggggcaa tcaatcagtt actggttatc
    14701 ttccatcatg accagttgca aagcatgttc ctgtggagca ccggaacgct gacgcaaacc
    14761 gactggggcg gtgttgagcg tttatggccg cagttgctgg gcggtgtgat gctgacgttg
    14821 ctgctacttc gtccgttaac cctgatgggg cttgatgatg gcgtggcgcg caatctcggg
    14881 ctggccttgt cgcttgcgcg tctggcggcg ctgtcgctgg cgattgtcat cagtgcgctg
    14941 ctggtgaacg ctgtggggat tatcggcttt atcgggttgt tcgcgccgct gctggcaaaa
    15001 atgctggggg cgcggcgtct gctgccacga ctgatgctgg cgtcgttgat tggtgcgctg
    15061 atcctctggc tttccgatca aatcatcctc tggctgactc gcgtgtggat ggaagtgtcc
    15121 accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggctgtt gccgcgttta
    15181 cgcagcatta gcgcgccgga tatgaaggtc aacgatcgtg tcgcggctga acgccaacat
    15241 gtgctggcgt ttgcccttgc tggcggtgtg ctgctgataa ttgctgtggt ggtggcgctg
    15301 tcgtttggtc gtgatgcgca cggctggacg tgggcgagcg gggcgctgct cgatgattta
    15361 atgccctggc gctggccgcg aattatggcg gcgctgtttg cgggcgtcat gctggcggta
    15421 gcgggctgta ttattcagcg gctgaccggc aacccgatgg caagcccgga agtgctgggg
    15481 attagctccg gcgcagcgtt tggcgtggtg ttgatgctgt ttctggtgcc gggtaatgcc
    15541 tttggctggc tgttacctgc aggcagtctc ggggcggcgg tgacgctgtt gatcattatg
    15601 atcgccgccg gacgcggtgg attttcccca caccgcatgt tactggcggg gatggcgtta
    15661 agtaccgcgt tcaccatgct tttgatgatg ttgcaggcaa gtggtgaccc gcgaatggcg
    15721 caagtgctga cctggatttc cggttcgacc tacaacgcga ccgatgcgca ggtctggcgc
    15781 acgggaattg tgatggtaat tttgctggcg attaccccgc tgtgccgccg ctggctgacc
    15841 attttaccgc taggtggtga taccgcccga gccgtaggaa tggcgctgac gccgacgcga
    15901 attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacgatgac tattggaccg
    15961 ttgagttttg ttggtttaat ggcaccgcat attgcgcgga tgatgggctt tcgacggacg
    16021 atgccacaca tcgtaatttc agcgctggtg ggtggtttac tgctggtatt cgctgactgg
    16081 tgtgggcgga tggtgctgtt tccattccag atcccagcgg ggctgctgtc gacctttatc
    16141 ggcgcgccat attttatcta tttgctgaga aagcagagcc gttaattttt ccatataacg
    16201 gccctgtacg cctggacgga taaagcgttt acgcgacaac ggatgcccga tgcgacgctg
    16261 gcgcgtctta tcgggcctac aaacggcccc gaatcgtagg tcggataagg cgttcacgcc
    16321 gcatccgaca aaccatgctg gatcacaact tcgcaaacac ccgacgtgca gcatcgatgg
    16381 tgttattgat atcttctatg ctgtgcgcca cggacataaa gcccgcttca aacgctgacg
    16441 gtgccaggta aacaccttcg tccagcatca tatggaagaa acgcttaaag cgttccacgt
    16501 cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag aaaataccga
    16561 acatgccgcc aacgtggtta acgaccagcg gaattccggc ttcttctgcc gcttccagca
    16621 gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaacgccc ggctgcgcga
    16681 cttcattcag acaggcgaaa cccgctgcca tcgcaattgg gttaccggaa agcgtacccg
    16741 cctgatagac cggacccgtc ggggccagcg catccattac atcacgacga ccaccgaatg
    16801 cgcctaccgg cattccaccg ccgatgattt tgcccaggca ggtgagatcc ggttccacac
    16861 cgtaataatc ctgtgcgcca gctagcgcta cgcggaagcc agtcattact tcatcgatga
    16921 tcagcaatgc gccaaattcg tcgcacagcg cgcgcagacc tggcaggaac tctggcagcg
    16981 gtggaacgca gttcatattg cctgccaccg gctcgacgat aatacaggca atctcttgcg
    17041 ggtattgctc aaacgcggcg cgtacagaag ccagatcgtt ataagtacag gttaaggtat
    17101 gtttggcgaa atctgccgga acacccggcg agtttggctg gcctaacgtg agtgcgccag
    17161 aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca aatttaatga
    17221 ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc gcttcagtgc
    17281 cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc agttgcgcca
    17341 ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca
    17401 cggcattgcg gatagccgga tggttatggc ccagcaccat cggcccccag gaaccgacat
    17461 aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct ttttcgataa
    17521 acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga
    17581 tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt tcctgattcg
    17641 tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata ctcgtcaaac
    17701 atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt ttgtgcattt
    17761 gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta
    17821 tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt agaaacaccg
    17881 caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg cgataaaacc
    17941 ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttgtcgggct ggcagcggtt
    18001 gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct ggtacatact
    18061 gctgataatt atccgcttct gttaaccgtc gcttttctct gttcggcggt gctggcgatg
    18121 ttcggctact ttttggtgcg caaatacgcg ccggaagcag gtggttcggg gatcccggaa
    18181 attgaagggg cgctggaaga tcaacgtccc gttcgctggt ggcgtgtgtt gccggtgaaa
    18241 ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga aggaccaacc
    18301 gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct gaaaggtgat
    18361 gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc ggcctttaac
    18421 gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt tcgctatacg
    18481 ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat gtaccggatt
    18541 tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc gcttaatacg
    18601 ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat ttttaataaa
    18661 tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg
    18721 gtactgatgg gtggtgcgat tggcggtctg tgtggattgc tggggtttgt ggcaccagca
    18781 acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt cagcatggga
    18841 atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt ctcttccggc
    18901 gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg aaccgctttc
    18961 ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac gtttgctatt
    19021 gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg gatcattctg
    19081 gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac cggtcttggc
    19141 gcaacactat tagcgcaatt taccggcggg aaaccgctat actcggcgat tcttgcgcgc
    19201 acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc agccagcgag
    19261 aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg ttagaatttg
    19321 cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact gccgctggag
    19381 tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga taacccgaat
    19441 ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta tggtttcacc
    19501 tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt tggcctggtg
    19561 gttgatccga tgagcctgca atatctggtc ggcggttccg ttgattatac cgaaggtctg
    19621 gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct
    19681 tcctttagta tctaatctgt tgttcatgtg ccggataaac tatccggcac atcttctctc
    19741 ttcaacgccc attctcatcc agcgcaaatg tcggtagctt aagatgccaa cgaatagccg
    19801 ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc agtggtacgg
    19861 aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca gttgcgtaga
    19921 tttctgtacg taaaatcatg gggatttcgc gggccagtac atcacgaatg atcccgccgc
    19981 caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc gcattaaagg
    20041 ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc accggcaaca
    20101 tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg ctggtgacca
    20161 ttgcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga tccagcgcca
    20221 tgtcgcgaat tgttccgccg cctactgcgg taaccacgcc cagtaccaga acaccaaaag
    20281 ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc gctgtgccga
    20341 ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga aaaggtcagg
    20401 cactaatcta cctgtgaaag cgcattacag agctgttgtg cagcgaggat aatacgtgtg
    20461 cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag ctgttcaccc
    20521 cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat cgcctgtggc
    20581 gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct gtctttaaag
    20641 atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt tccactggta
    20701 aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc agcatattgc
    20761 gctttcaatt gcgcgtactg atccagcagg gattgcgcgg cttgttcggc cttgtctggt
    20821 tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca
    20881 tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg ctcggcatta
    20941 cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc cagattcatc
    21001 ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg
    21061 ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc gggagaaagc
    21121 gtgatgacgc gcggcgcggc gttgagccac agtggcgcaa gaaaagacag ggcgaccagc
    21181 gccctgaaca gtgacttagc catgtgcaag tttctgcacc agtgactcaa ccatcaggct
    21241 ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact gttgatcggc
    21301 cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc agacatgggc
    21361 gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc ggattttcgc
    21421 cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt
    21481 aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt cagctttaaa
    21541 gcctgccgga cagcctggta actgaccgta ttcataacca aatgccgtga catccgcgtc
    21601 gtgataacgt gcttcgtccg agacaacgat atcgcccact ttcaacgttg gtgccaggcc
    21661 accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca gcaaagtggc
    21721 acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct cggttccatt
    21781 cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt tttcgatttt
    21841 gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt tcatagattt
    21901 actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg aagcgtattt
    21961 ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac tggcatcgtc
    22021 gttaccgttc accgcagggc gttaaaaccg gacatgagat cctgcggatc ttcgagagcg
    22081 atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt
    22141 ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc
    22201 aggtggggcg ctacatcgcc aaagaaattc taagccgtct gaaagagctt aaattactgg
    22261 aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag atgtcatgcc
    22321 tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcggcg ataaatgatt
    22381 ggtttcgcca gcgtttgcac ccggaagatg ccgaaagcca gcctctgact gacgatcgct
    22441 gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag ctgcggcgca
    22501 agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt ctggtgcata
    22561 cattgatgag gatgaatctc acctgggcac aggttggcgg tattttaaaa tatacccgtc
    22621 cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa aagccgggtt
    22681 attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat ttggcgcttt
    22741 acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc tattgtgtgg
    22801 cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt tatcatcatt
    22861 tgcacgaggc gtggggtcag catgagaaag gttcgctctt ttcgctagtg gttgaaaatg
    22921 cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag ttttttatgt
    22981 atttacgggt aaacacccta aataaactgg taccatacgc ggcacaacga tttattgata
    23041 atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat gccagcgaat
    23101 gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt agccatccag
    23161 atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta gagatttatc
    23221 gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa gaacgggtga
    23281 aacgtttccc tattgaatcg cgcttattcc acaaactctc gacgcgccat cggctggcct
    23341 atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta tgggaatatt
    23401 attaccgttg ccgcctgttg caggattata tcagcggtat gaccgacctc tatgcgtggg
    23461 atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa gacgaacaat
    23521 aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa cgaatctgaa
    23581 gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa aaaaccacat
    23641 tagcactgag tgtactggct ctgagtttag gtttggcgtt atctccgctc tctgcaacgg
    23701 cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca ccgatgctcg
    23761 aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc gttaatacgc
    23821 cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc tgccaggaag
    23881 gttcaccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat ggcggcggcc
    23941 agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat aaaggctatg
    24001 tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag ctgagcgatg
    24061 gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc gcgctgattc
    24121 agatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat gcactgcgcg
    24181 tgggtgatta caccgtagcg attggtaacc cgtttggcct gggagagacg gtaacttccg
    24241 ggattgtctc tgcgctgggg cgtagcggcc tgaatgccga aaactacgaa aacttcatcc
    24301 agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac ctgaacggcg
    24361 aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc ggtatcggtt
    24421 ttgctatccc gagcaacatg gtgaaaaacc tgacctcgca gatggtggaa tacggccagg
    24481 tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg gcgaaagcga
    24541 tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat tcctccgctg
    24601 caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag ccaatcagca
    24661 gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa ctgaccctgg
    24721 gcttgctgcg cgacggtaag caggtcaacg tgaatctgga actgcagcag agcagccaga
    24781 atcaggttga ttccagctcc atcttcaacg gcattgaagg tgctgagatg agcaacaaag
    24841 gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg cactccggct gcgcagatcg
    24901 gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa aacatcgctg
    24961 aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt cagcgcggcg
    25021 acagctccat ctacctgtta atgcagtaat ctccctcaac cccttcctga aaacgggaag
    25081 gggttctcct tacaatctgt gaacttcacc acaactccat acatcttcat catcctttag
    25141 gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca taacggagga
    25201 cttatggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt accatgcgca
    25261 tcatcgatac caatatcaac gtaatggatg cccgtgggcg aattatcggc agcggcgatc
    25321 gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag ggacgagtcg
    25381 tcgatatcga tgacgcggtg gcacgtcatc tgcacggtgt gcggcaaggg attaatctac
    25441 cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc
    25501 tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt
    25561 tgatgcactt gttggctcag gatagccgtt tgcgggagga actggtgatg aacctgattc
    25621 aggcagagga gaatactccc gcacttactg aatgggcgca acggctgggg atcgatctca
    25681 atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggt gtggacagcg
    25741 caatggcgga gttacaacaa ctgcaaaacg cgctgactac gcccgagcgt aataatctgg
    25801 tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac tcttttgggc
    25861 gctgggatgc agaagatcat cgtaagcgag ttgaacaact gattacccgc atgaaagagt
    25921 acggccagct gcgttttcgc gtttcactgg gcaactattt taccggtcct ggcagtattg
    25981 cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg atgccagaaa
    26041 gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt ttgcgtggcg
    26101 actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg gacaataacg
    26161 gcttgctgcg acgaacgctg gcggcgtggt ttcgtcacaa tgtgcaaccg ctggcaacgt
    26221 caaaggcgtt gtttattcat cgtaataccc tggagtatcg gcttaatcgt atatcggaac
    26281 tgaccgggct tgatttgggc aattttgatg acaggttgct gctgtatgtg gcgttacaac
    26341 tggatgaaga gcggtaggtt atgcgttaag gttgaggcgg cgctggctca ttcccctcag
    26401 cccgaagggg agaggggaat gcccgaatta accgtcaatt cgggattatt tacgcgttag
    26461 tttttccaga tcggcttcaa tctcgctgat cttattggtt acgactgact ccaggtgacg
    26521 taagtcgtcg aggatcttac gcttaagatc aacttcgctg cggtcacgct ggcagatttg
    26581 atcaagctca tcaatgatat accgtagatt cgggctgatt tcctggactt ctttataacc
    26641 ctgacccaca ccatcagcga cgaccgtttt acgctgacgc ggatatttaa acttaacgct
    26701 cttggcgaaa aactcgcctt tgtctttctg gaaatagatt ttcagaatat cgttgttggc
    26761 ttcctgccgg aggctgtaac gatcaatttc ttcaggattg gtaataccca gacttttcag
    26821 attgtcgtac atagcgttac ctcaaaatga gtcagtaaat tgtgcttatt ttagcatttg
    26881 gcctggcccg cccggctcga ctgtgatcgc agaaagcctt tttcttccag acttctatca
    26941 tggcgtaaaa atcaaaaaat tacctgcttt attgtggtga taaaattcac gttctacacc
    27001 agttccagca tcgttacttc cgggcggcag ttcagacgca aaccatacaa actgcccacg
    27061 cgacgggttg tgtagatgtg tcgttcgcca aaggcattta atccggcgac gtaacgttta
    27121 tcttcgacag gggcaaaagg ttcaccaacc aacggtacgc gcagttgccc gccgtgagta
    27181 tggccgcaca gcatcagatc ccagggttca tcacgcatga cttctttgct gtcgggatta
    27241 tgtgccagca tcagtcttgg cagatttgct tcgctggcgg gaggtggttt gcattgtccg
    27301 gcccataagt caccggtgcc taccagttcg aattgcctgt tcggcgtggc gatcaccgta
    27361 gcctggttaa acaacaccgt gatgcccact gatttcaacg tctcgccaat taagtgattt
    27421 ttttctgtac caacagggcg atcgtggttg ccgaagcagg caaacgtcgg cgcacattcg
    27481 gcaagggggg agaggacatc actaaacgcc gaaaaattca gcggcatatc aaataatacg
    27541 taatcgccgc ccagtaatat caaatcgggc ttttgttcta tgccaagagc aatcgcgtca
    27601 gaaatcaggc ttaaaggaac aaaacgagag taatggagat cggccagaaa aagaattttg
    27661 aatggtgctg cgttgtcttt aaaaaaggcg aggcggtgac ggattaattc aaaccagcct
    27721 ggctcccagt aatgcatata accgaaacct gagctcgtgg ctatcgtcgc ggcagtagcc
    27781 tgcaacaatc ggcggcgtga aatcatcgct catccctgca aaaaaaatcg ggcagcgtcg
    27841 tgctgcccgt gtgcatactt ttagtcgatg gtacgcagca gttcgttaat gccgactttg
    27901 ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac agtagaggct gtatttgcca
    27961 tcttttgacg gcagattacc tgaaacaacc acagaccccg ccggaacgcg accgtagtgg
    28021 atttcgccgg tttcacggtc gtaaatacgg gtgctctgac caatgtatac gcccatggaa
    28081 atgacggaac cttcttcgac aatcacccct tcaaccactt cagagcgtgc gccaatgaag
    28141 cagttatctt caatgatggt tgggttagcc tgcagcggtt ccagcacgcc gccgatgccc
    28201 acgccaccgg aaaggtggac gtttttacca atctgcgcac aagaaccgac ggtcgcccag
    28261 gtatcaacca tggtgccttc atcaacatat gcgccgatgt tgacgtaaga cggcatcagc
    28321 acggtgttac gggcaataaa cgcaccctga cgtaccgccg ctggtggcac aacgcggaag
    28381 ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt tcatcggcac tttgtcgaag
    28441 tagcggcttt ctgccccttc gatcacctga ttatcattaa tacggaaaga gagcagcacc
    28501 gcttttttca accactgatg cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt
    28561 gcaccggaat ccagcagggc gatcacctga tttaccgctt cgcgggtaac ggtgtctgcg
    28621 ttggctggcg tgatctcggc acggcgttca aaagcggttt caataatgtt ctgtaactgc
    28681 tgcattgtta aactcttttc atatcagtaa acacatcacc ctttatcgtt tggattgagg
    28741 gcctctgtca accgctgatg cacttcctgc tgcaactcgt tattaagtgc acgccggtca
    28801 gcggtggcaa ttatgaataa atcttctact cgctcgccaa tggttgtaat tcgggcacca
    28861 tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc tcgccagcag tcctggctgg
    28921 tcgagcgcga taagctcaag gaacgatttg cggtcggtat gggtcggcaa aaacgttact
    28981 tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc gacggggctg cggtggctgc
    29041 cagctacttt gcgtcagtac ttgctccaga ccaaaccgaa taacctcatg acgatctgcg
    29101 gacagcgggc tgccatcggg ttccagcacg ataaaggaat ccatcgccat accgtcgcga
    29161 gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc ggcacagacg
    29221 gcggcaaaca gataggggcg gtccgggctc cagataaaaa tctcggtgcc tccacgcgta
    29281 gcctgcgggc taagcaatac cagcggtttg cttaaatcat gctgtaataa atggcgggca
    29341 tgccaggcca gttgatttgg gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa
    29401 atttggtgca gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag tgcctggagt
    29461 tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc
    29521 ttttcggtgg caaaatagag ttcgcgcaac agactttgct tccagctatt ccacagcgtt
    29581 tcgttggtgg cgcaaatgtc agccactgtc aggcatacca gatagcgcag acgattttcc
    29641 gtttgaactt cttcggcaaa ctgcttgatg acttcagggt cctgaatatc gcggcgttgt
    29701 gctgtcaccg acatcagcaa gtgctggcga accagccagg caaccagctg tgtttcgcgt
    29761 gagttcagcc cgtggagttc tgcaaaatgc actacatcct gagcaccgag aatggagtgg
    29821 tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt
    29881 gacggcaggc gcggccagac gtccacacac aacggatggc gctggcgcgt ttcttcactg
    29941 gcaaaactct ccagtttcag catcacgcgg atagtatgtt catccaccgt gtaggcgtgg
    30001 aacagatcaa actgcatctg cccgacgata tgcgaccatt gcggcatata cgcgccgagc
    30061 acgctatggc gatgcattgg caatagcccg cgccgcaccg ctccggggtg acgcagaatg
    30121 ctcaaaaaca gttttcgtgc ttccggaata ttacacagcg gttgttgcag atggcgacgg
    30181 gcatggcgta actggcgcag cgtggtggag taaatgccgg tgatcgcact gttgcgcacc
    30241 atggtgtaga acatacgcaa gatggcttcc ggctggcgca taaatagtgt ttcatcacgc
    30301 aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga ttggacgtgg tttttcgtcg
    30361 gcgggaaggg cgaggatggc ttcatcgaac agttgcagca gcatctggtt gagttcactg
    30421 acgcggcgtg taacgcggaa gtaatccttc atcatccgct cgaccggttc gttgccttca
    30481 ccactgtaat tcagacgctg agcgacgcta agttggcgat cgaataacag gcgattatcg
    30541 taacggctga cgaccagatg cagggcaaag cgaatacgcc acagtatatg cagacattcg
    30601 tttaattccg cccgctccgc tgaggttaag aagccaaacc caaccatttc atccagcgat
    30661 gttgcgccaa aatgacggcg ggccacccat tgcagagtgt ggatatcgcg caagccgcca
    30721 gggctacttt tgatgtctgg ttcaagattg tagctggtgc cgtggtaacg ctgatggcgc
    30781 tggttctgtt cttcaacttt cgccgcgtag aacttgtcgg aaggccagaa tccttcgcta
    30841 aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc caattaataa gcgggattcg
    30901 attaaattgg tggcgacggt taaatccgat aacccttcca gcatgcactc ttcaagcgtg
    30961 cgcacactat gaccgacttc cagctttaca tcccagagca gcgttaacag ctcgcccact
    31021 ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa
    31081 agtggatgca gctcgccacg accgtagcca ccgacggcga ccaatgccag gtcggcaatc
    31141 tggctgaatc ccgcttcaat ccataatcgt tgcaggagct ggtcgataaa ctcggtgcgc
    31201 gcctcaatca actgttctgc agagatccca ttgtcaaagg catcacccag ccaacgctgg
    31261 aaagtatcga tatgggcttt tatcccaccg acggttaatt catcacgggg ccagacgcat
    31321 ggattttgcg gttgaccggg cagggtgggg agagcggtgt ttgcgtactg ttctggaagg
    31381 gtattcattg tgcgccaccc ataagattaa attatcgcat taaaaaagcc ggcattcgcc
    31441 ggcttcatct tattcgtcgt gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt
    31501 cagaatttcg cagccgttat cagtcaccac aatagtatgc tcatattgtg cagacaagct
    31561 gcgatctttg gtttttaccg tccagccatc tttcatggtg cggatctctt ttttacccgc
    31621 gttgaccatt ggctcgatgg tgaacgtcat cccaggtttc agtacgacgt tggtttcacg
    31681 ggagtcatag tgcagcacct gcggttcttc atggaagccg cgaccaatac cgtgtccgca
    31741 atattcacga acgacggaga agccttctac ttcgacaaac ttctgaatcg ccgcaccgat
    31801 ttcgcgcagg ttaatgcctg gtttgaccat gcgcagcgcc aggtacaggc tttcttgcgt
    31861 gatgcggcac agacgttcgc ccatgatggt cggcttaccg acgataaaca ttttcgaggt
    31921 atcgccgtgg aaaccatctt tgattacggt gacatcaatg ttaacgatat cgccatcttt
    31981 cagcagcttc gcatcgtccg ggataccgtg gcacaccact tcattaatag agatgcaaac
    32041 ggatttcgga tagccgtgat agccgaggca ggcagaaacc gcgtgttgtt cattaacaat
    32101 gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa catacggttc
    32161 gatcatctcc agcacttcgg cagccagtcg gccagcgacg cgcatttttt cgatatcttc
    32221 tggggtcttg attgagatag ccattaattc tgtccatcag cgtcggtgat accgacaata
    32281 tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat tctgaatttc
    32341 gccaaacgtg ccactgaacg ttttctataa tagaaaattc aacgtctgat gctgtacaca
    32401 gcgccaacaa ttattggtgt ccatgacgta tttgtggtat aaagcgcgcc ggacttccga
    32461 tccatttcgt atacacagac tggacggaag cgacaatctc actttgtgta acaacacaca
    32521 cgtatcggca catattccgg ggtgcccttt ggggtcggta atatgggata cgtggaggca
    32581 taaccccaac ttttatatag aggttttaat catggcaact gtttccatgc gcgacatgct
    32641 caaggctggt gttcacttcg gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt
    32701 catcttcggt gcgcgtaaca aagttcacat catcaacctt gagaaaactg taccgatgtt
    32761 caacgaagct ctggctgaac tgaacaagat tgcttctcgc aaaggtaaaa tccttttcgt
    32821 tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct gcgaccagtt
    32881 cttcgtgaac catcgctggc tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc
    32941 catcaaacgt ctgaaagacc tggaaactca gtctcaggac ggtactttcg aaaagctgac
    33001 caagaaagaa gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg
    33061 tatcaaagac atgggcggtc tgccggacgc tctgtttgta atcgatgctg accacgaaca
    33121 cattgctatc aaagaagcaa acaacctggg tattccggta tttgctatcg ttgataccaa
    33181 ctctgatccg gacggtgttg acttcgttat cccgggtaac gacgacgcaa tccgtgctgt
    33241 gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc aggatctggc
    33301 ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa ggcttgataa ctcccccaaa
    33361 atagttcgag ttgcagaaag gcggcaagct cgagaattcc cgggagctta catcagtaag
    33421 tgaccgggat gagcgagcga agacaacgca tctgcggcgc gaaatatgaa gggggagagc
    33481 ccttatagac caggtagtac acgtttggtt agggggcctg catatggccc cctttttcac
    33541 ttttatatct gtgcggttta atgccgggca gatcacatct ccgaggattt tagaatggct
    33601 gaaattaccg catccctggt aaaagagctg cgtgagcgta ctggcgcagg catgatggat
    33661 tgcaaaaaag cactgactga agctaacggc gacatcgagc tggcaatcga aaacatgcgt
    33721 aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga cggcgtgatc
    33781 aaaaccaaaa tcgacggcaa ctacggcatc attctggaag ttaactgcca gactgacttc
    33841 gttgcaaaag acgctggttt ccaggcgttc gcagacaaag ttctggacgc agctgttgct
    33901 ggcaaaatca ctgacgttga agttctgaaa gcacagttcg aagaagaacg tgttgcgctg
    33961 gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg ctgcgctgga aggcgacgtt
    34021 ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg ttgctgctaa aggcgctgac
    34081 gaagagctgg ttaaacacat cgctatgcac gttgctgcaa gcaagccaga attcatcaaa
    34141 ccagaagacg tatccgctga agtggtagaa aaagaatacc aggtacagct ggatatcgcg
    34201 atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg catgaagaaa
    34261 ttcaccggcg aagtttctct gaccggtcag ccgttcgtta tggaaccaag caaaactgtt
    34321 ggtcagctgc tgaaagagca taacgctgaa gtgactggct tcatccgctt cgaagtgggt
    34381 gaaggcatcg agaaagttga gactgacttt gcggcagaag ttgctgcgat gtccaagcag
    34441 tcttaattat caaaaaggag ccgcctgagg gcggcttctt tttgtgccca tcttgtaaat
    34501 tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta gtattaattc
    34561 atttcaatcg ttgacagtct caggaaagaa acatggctac caatgcaaaa cccgtctata
    34621 aacgcattct gcttaagttg agtggcgaag ctctgcaggg cactgaaggc ttcggtattg
    34681 atgcaagcat actggatcgt atggctcagg aaatcaaaga actggttgaa ctgggtattc
    34741 aggttggtgt ggtgattggt gggggtaacc tgttccgtgg cgctggtctg gcgaaagcgg
    34801 gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg aacggcctgg
    34861 caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc gctattccat
    34921 tgaatggcgt gtgcgacagc tacagctggg cagaagctat cagcctgttg cgcaacaacc
    34981 gtgtggtgat cctctccgcc ggtacaggta acccgttctt taccaccgac tcagcagctt
    35041 gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa gttgacggcg
    35101 tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat gtacgagcaa ctgacttaca
    35161 gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc ggccttcacg ctggctcgtg
    35221 accataaatt accgattcgt gttttcaata tgaacaaacc gggtgcgctg cgccgtgtgg
    35281 taatgggtga aaaagaaggg actttaatca cggaataatt cccgtgatgg ataaataagg
    35341 gtaagattcc gcgtaagtat cgcgggggcg taagtctggt tataaggcgt tattgttgca
    35401 ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta caaaagtacg tgaggatggc
    35461 gagcactgct cggggccaaa atggcaaata aaatagccta ataatccaga cgattacccg
    35521 taatatgttt aatcagggct atacttagca cacttccact gtgtgtgact gtctggtctg
    35581 actgagacaa gttttcaagg attcgtaacg tgattagcga tatcagaaaa gatgctgaag
    35641 tacgcatgga caaatgcgta gaagcgttca aaacccaaat cagcaaaata cgcacgggtc
    35701 gtgcttctcc cagcctgctg gatggcattg tcgtggaata ttacggcacg ccgacgccgc
    35761 tgcgtcagct ggcaagcgta acggtagaag attcccgtac actgaaaatc aacgtgtttg
    35821 atcgttcaat gtctccggcc gttgaaaaag cgattatggc gtccgatctt ggcctgaacc
    35881 cgaactctgc gggtagcgac atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta
    35941 aagatctgac caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca gtacgtaacg
    36001 tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa agataaagag atcagcgaag
    36061 acgacgatcg ccgttctcag gacgatgtac agaaactgac tgatgctgca atcaagaaaa
    36121 ttgaagcggc gctggcagac aaagaagcag aactgatgca gttctgattt cttgaacgac
    36181 aaaaacgccg ctcagtagat ccttgcggat cggctggcgg cgttttgctt tttattctgt
    36241 ctcaactctg gatgtttcat gaagcaactc accattctgg gctcgaccgg ctcgattggt
    36301 tgcagcacgc tggacgtggt gcgccataat cccgaacact tccgcgtagt tgcgctggtg
    36361 gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg aattctctcc ccgctatgcc
    36421 gtaatggacg atgaagcgag tgcgaaactt cttaaaacga tgctacagca acagggtagc
    36481 cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct tgaggatgtt
    36541 gatcaggtga tggcagccat tgttggcgct gctgggctgt tacctacgct tgctgcgatc
    36601 cgcgcgggta aaaccatttt gctggccaat aaagaatcac tggttacctg cggacgtctg
    36661 tttatggacg ccgtaaagca gagcaaagcg caattgttac cggtcgatag cgaacataac
    36721 gccatttttc agagtttacc gcaacctatc cagcataatc tgggatacgc tgaccttgag
    36781 caaaatggcg tggtgtccat tttacttacc gggtctggtg gccctttccg tgagacgcca
    36841 ttgcgcgatt tggcaacaat gacgccggat caagcctgcc gtcatccgaa ctggtcgatg
    36901 gggcgtaaaa tttctgtcga ttcggctacc atgatgaaca aaggtctgga atacattgaa
    36961 gcgcgttggc tgtttaacgc cagcgccagc cagatggaag tgctgattca cccgcagtca
    37021 gtgattcact caatggtgcg ctatcaggac ggcagtgttc tggcgcagct gggggaaccg
    37081 gatatgcgta cgccaattgc ccacaccatg gcatggccga atcgcgtgaa ctctggcgtg
    37141 aagccgctcg atttttgcaa actaagtgcg ttgacatttg ccgcaccgga ttatgatcgt
    37201 tatccatgcc tgaaactggc gatggaggcg ttcgaacaag gtcaggcagc gacgacagca
    37261 ttgaatgccg caaacgaaat caccgttgct gcttttcttg cgcaacaaat ccgctttacg
    37321 gatatcgctg cgttgaattt atccgtactg gaaaaaatgg atatgcgcga accacaatgt
    37381 gtggacgatg tgttatctgt tgatgcgaac acgcgtgaag tcgccagaaa agaggtgatg
    37441 cgtctcgcaa gctgaggata atccggctac agagagtcgc gctatttgtt agcgtagggc
    37501 ttcagtgata tagtctgcgc catctgatcg taagtagttg gctttataag gtcaggtatg
    37561 ccgtggtttt acacggcttt tttttgtata ggcttcagta ttcctgagta ccgtaaaccc
    37621 tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca accacttagc gaaaaattgc
    37681 cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa
    37741 agcaagggaa gattcgtgcc tttgggcata aagccggggc aaaatccgtc cgccgggctg
    37801 tctcttttgc ggccaataac ggtattgagg cgttaacgct gtatgccttt agtagtgaaa
    37861 actggaaccg accagcgcag gaagtcagtg cgttaatgga actgtttgtg tgggcgctcg
    37921 atagcgaagt aaaaagtctg caccgacata acgtgcgtct gcgtattatt ggcgatacca
    37981 gtcgctttaa ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta acagccggga
    38041 ataccggtct gacgctgaat attgcggcga actacggtgg acgttgggat atagtccagg
    38101 gagtcaggca actggctgaa aaggtgcagc aaggaaacct gcaaccagat cagatagatg
    38161 aagagatgct aaaccagcat gtctgtatgc atgaactggc ccctgtagat ttagtaatta
    38221 ggactggggg ggagcatcgc attagtaact ttttgctttg gcaaattgcc tatgccgaac
    38281 tttactttac agatgttctc tggcccgatt tcgatgaaca agactttgaa ggggcgttaa
    38341 atgcctttgc taatcgagag cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct
    38401 gatggggggt cgcttttgct gaagtatcgc ctgatatctg cttttgtgtt aatacccgtc
    38461 gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg ccatcgtaac gctggtggtc
    38521 tgcatgctgg cagcgtggga atggggacag cttagcggtt ttaccactcg tttgcagcga
    38581 gtatggttgg cggtgttatg cgggttattg ttggcgctga tgctttttct gttgccggaa
    38641 tatcaccgaa atattcatca accgctggtt gaaatctcac tttgggcttc gctgggttgg
    38701 tggattgtcg cgctattgct ggtgctgttt tacccaggtt ccgcagcaat ctggcgtaac
    38761 tctaaaacat tgcgccttat ttttggcgtg ctaaccattg ttcccttctt ctggggcatg
    38821 ctggcgttac gggcctggca ctacgacgag aatcattaca gtggcgcaat atggctgctc
    38881 tatgtcatga tcctggtatg gggcgctgac tctggcgcat atatgtttgg caaattgttt
    38941 ggtaaacata agctggcacc gaaggtttct ccgggtaaaa cctggcaagg ctttataggt
    39001 ggactagcga ccgcagcggt aatctcatgg ggttatggca tgtgggcgaa tctcgacgtc
    39061 gctcccgtca ccttactcat ttgctctatt gtcgcagcgt tagcctcagt gctcggcgat
    39121 ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg acagcggtca tttaattcca
    39181 ggacacggtg gtattttaga tcgtattgat agcctgacgg ctgcggtacc ggtctttgct
    39241 tgcttgttgt tactggtatt caggactctt taacggaagg taatatgctg agttttctct
    39301 gggatttggc ttcgttcatc gttgcactgg gtgtacttat caccgtgcat gaatttggtc
    39361 atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg tttctcaata gggtttggta
    39421 aggcgctctg gcggcgaact gataagctcg gcaccgaata tgttatcgcc ctgatcccgc
    39481 tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc ggtcgttccg gaactccgcc
    39541 accatgcctt caataataaa tctgttggcc aacgagcggc gattattgcc gcaggtccgg
    39601 ttgcaaactt catttttgct atctttgcct actggctggt ttttattatt ggtgtgcctg
    39661 gcgtacgtcc ggtggttggt gaaatagcag ccaattcgat agctgcggaa gcacaaattg
    39721 caccaggtac ggaactaaaa gccgtagatg gtatcgaaac gcctgattgg gatgccgtgc
    39781 gtttgcagtt ggtcgataaa attggcgatg aaagcaccac cattacagta gcgccatttg
    39841 gcagcgatca acggcgggat gtaaagctcg atttacgtca ctgggcgttt gagcctgata
    39901 aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg gccgcaaatt gaacctgtac
    39961 tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg tttgcaagca ggcgacagga
    40021 tcgttaaagt cgatggtcag cccttaacgc agtgggtgac ctttgtgatg cttgtccggg
    40081 ataacccggg taaatcctta gcgttagaaa tcgaaaggca gggaagtccc ttgtctttga
    40141 cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat tggttttgtc ggtattgagc
    40201 cgaaagtcat tcctttgcca gatgagtata aagttgtacg ccagtatggg ccgttcaacg
    40261 ccatcgtcga agccacggac aaaacgtggc agctgatgaa gctgacggtc agtatgctgg
    40321 gaaaattgat caccggtgat gtgaaactga acaacctcag tgggccgatc tctatcgcca
    40381 agggggctgg gatgacagcg gaactcgggg tagtttatta cctgccgttt cttgcgctta
    40441 ttagcgtgaa cttagggata attaacctgt ttccgttgcc cgtacttgac ggggggcatc
    40501 tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt atacgagcgg gttcaagact
    40561 tttgttatcg cattggctcg attctgctgg tgctgttaat ggggcttgca cttttcaatg
    40621 atttctctcg gttatgagag ttagttagga agaacgcata ataacgatgg cgatgaaaaa
    40681 gttgctcata gcgtcgctgc tgtttagcag cgccaccgta tacggtgctg aagggttcgt
    40741 agtgaaagat attcatttcg aaggccttca gcgtgtcgcc gttggtgcgg ccctcctcag
    40801 tatgccggtg cgcacaggcg acacggttaa tgatgaagat atcagtaata ccattcgcgc
    40861 tctgtttgct accggcaact ttgaggatgt tcgcgtcctt cgtgatggtg atacccttct
    40921 ggttcaggta aaagaacgtc cgaccattgc cagcattact ttctccggta acaaatcggt
    40981 gaaagatgac atgctgaagc aaaacctcga ggcttctggt gtgcgtgtgg gcgaatccct
    41041 cgatcgcacc accattgccg atatcgagaa aggtctggaa gacttctact acagcgtcgg
    41101 taaatatagc gccagcgtaa aagctgtcgt gaccccgctg ccgcgcaacc gtgttgacct
    41161 aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag caaattaaca ttgttggtaa
    41221 ccatgctttc accaccgacg aactgatctc tcatttccaa ctgcgtgacg aagtgccgtg
    41281 gtggaacgtg gtaggcgatc gtaaatacca gaaacagaaa ctggcgggcg accttgaaac
    41341 cctgcgcagc tactatctgg atcgcggtta tgcccgtttc aacatcgact ctacccaggt
    41401 cagtctgacg ccagataaaa aaggtattta cgtcacggtg aacattaccg aaggcgatca
    41461 gtacaagctt tctggcgttg aagtgagcgg caaccttgcc gggcactccg ctgaaattga
    41521 gcagctgact aagatcgagc cgggcgagct gtataacggc accaaagtga ccaagatgga
    41581 agatgacatc aaaaagcttc tcggtcgcta tggttatgcc tatccgcgcg tacagtcgat
    41641 gcctgaaatt aacgatgccg acaaaaccgt taaattacgc gtgaacgttg atgcgggtaa
    41701 ccgtttctac gtgcgtaaga tccgttttga aggtaacgat acctcgaaag atgccgtcct
    41761 gcgtcgcgaa atgcgtcaga tggaaggtgc atggctggga agcgatctgg tcgatcaggg
    41821 taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc gataccgata cccaacgtgt
    41881 tccgggtagc ccggaccagg tagatgtcgt ctacaaggta aaagagcgca acaccggtag
    41941 cttcaacttt ggtattggtt acggtactga aagtggcgtg agcttccagg ctggtgtgca
    42001 gcaggataac tggttaggta caggttatgc tgttggtatc aacgggacca aaaacgatta
    42061 ccagacctat gctgaactgt cggtaaccaa cccgtacttc actgtagatg gtgtaagcct
    42121 cggtggtcgt ctcttctata atgacttcca ggcagatgac gccgacctgt ccgactatac
    42181 caacaagagt tatggtacag acgtgacgtt gggcttcccg atcaacgaat ataactcgct
    42241 gcgtgcaggt ctgggttatg tacataactc cctgtccaac atgcagcctc aggttgcgat
    42301 gtggcgttat ctgtactcta tgggtgaaca tccgagcacc tctgatcagg ataatagctt
    42361 caaaacggac gacttcacgt tcaactatgg ttggacctat aacaagcttg accgtggtta
    42421 cttcccgaca gatggttcac gtgtcaacct gaccggtaaa gtgaccattc ctggatcgga
    42481 taacgaatac tacaaagtga cgttagacac ggcgacttat gtgccgatcg atgacgatca
    42541 caaatgggtt gttctggggc gtacccgctg gggttatggt gatggtttag gcggcaaaga
    42601 gatgccgttc tacgagaact tctatgccgg tggttccagc accgtgcgtg gcttccagtc
    42661 caatacaatt ggtccgaaag cagtttactt cccgcatcag gccagtaatt atgatccgga
    42721 ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac ctgtgtaaat cggatgatgc
    42781 tgtaggcggt aacgccatgg cggttgccag cctcgagttc atcaccccga cgccgtttat
    42841 tagcgataag tatgctaact cggttcgtac ttccttcttc tgggatatgg gtaccgtttg
    42901 ggatacaaac tgggattcca gccaatattc tggatatccg gactatagtg atccaagcaa
    42961 tatccgtatg tctgcgggta tcgcattaca atggatgtcc ccattggggc cgttggtgtt
    43021 ctcctacgcc cagccgttca aaaagtacga tggagacaag gcagaacagt tccagtttaa
    43081 catcggtaaa acctggtaag tgttctccac aaaggaatgt agtggtagtg tagcgatgac
    43141 tttaggcgat caatataaga tcgccgggcc acgcaaagaa ctgcaccctc cggtgcaaat
    43201 gggatggtaa ggagtttatt gtgaaaaagt ggttattagc tgcaggtctc ggtttagcac
    43261 tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt caacatgggc agcctgttcc
    43321 agcaggtagc gcagaaaacc ggtgtttcta acacgctgga aaatgagttc aaaggccgtg
    43381 ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa aatgaaaaag ctgcagtcca
    43441 tgaaagcggg cagcgatcgc actaagctgg aaaaagacgt gatggctcag cgccagactt
    43501 ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg
    43561 gcaaactggt tactcgtatc cagactgctg tgaaatccgt tgccaacagc caggatatcg
    43621 atctggttgt tgatgcaaac gccgttgctt acaacagcag cgatgtaaaa gacatcactg
    43681 ccgacgtact gaaacaggtt aaataagtaa tgccttcaat tcgactggct gatttagcgc
    43741 agcagttgga tgcagaacta cacggtgatg gcgatatcgt catcaccggc gttgcgtcca
    43801 tgcaatctgc acaaacaggt cacattacgt tcatggttaa cccaaaatac cgtgagcatt
    43861 taggcttgtg ccaggcgtcc gcggttgtca tgacccagga cgatcttcct ttcgcgaaaa
    43921 gtgccgcgct ggtagtgaag aatccctacc tgacttacgc gcgcatggcg caaattttag
    43981 ataccacgcc gcagcccgcg cagaacattg cacccagtgc ggtgatcgac gcgacggcga
    44041 agctgggtaa caacgtatcg attggcgcta acgcggtgat tgagtccggc gttgaactgg
    44101 gcgataacgt gattatcggt gccggttgct tcgtaggtaa aaacagcaaa atcggtgcag
    44161 gttcgcgtct ctgggcgaac gtaaccattt accatgagat ccagatcggt cagaattgcc
    44221 tgatccagtc cggaacagtg gtaggcgcag acggctttgg ttatgccaac gatcgtggta
    44281 actgggtgaa gatcccacag attggtcgcg taattattgg cgatcgcgtg gagatcggtg
    44341 cctgcacaac catcgatcgc ggcgcgctgg atgacactat tattggcaat ggcgtgatca
    44401 ttgataacca gtgccagatt gcacataacg tcgtgattgg cgacaatacg gcggttgccg
    44461 gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta ctgcatgatc ggcggagcca
    44521 gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac ggttacgggc atgggtatgg
    44581 tgatgcgtcc catcactgaa ccaggcgtct attcctcagg cattccgctg caacccaaca
    44641 aagtctggcg caaaaccgct gcactggtga tgaacattga tgacatgagc aagcgtctga
    44701 aatcgcttga gcgcaaggtt aatcaacaag actaacgttc catcttttgt tcgccaaact
    44761 ttacggcctg tctcattctt acgattgcgg caggccgtgt tattattgtc gtttcttata
    44821 ttttgacagg aagagtatct tgactactaa cactcatact ctgcagattg aagagatttt
    44881 agaacttctg ccgcaccgtt tcccgttctt actggtggat cgcgtgctgg attttgaaga
    44941 aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat gagccattct tccagggcca
    45001 tttccctgga aaaccgattt tcccgggtgt gctgattctg gaagcaatgg cacaggcaac
    45061 aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg ggtgagctgt actacttcgc
    45121 tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct ggcgatcaaa tgatcatgga
    45181 agtcactttc gaaaaaacgc gccgcggcct gacccgtttt aaaggggttg ctctggtcga
    45241 tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt agccgggagg cctgatacgt
    45301 gattgataaa tccgcctttg tgcatccaac cgccattgtg gaagagggcg cgtcaattgg
    45361 cgcgaacgca cacattggtc ctttttgtat cgttggaccc catgtcgaaa ttggtgaggg
    45421 taccgtactg aaatctcacg ttgtcgtgaa tggtcatact aaaattggcc gcgataatga
    45481 gatttatcag ttcgcctcca tcggcgaagt taaccaggat ctgaaatatg ctggcgaacc
    45541 gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa agcgtcacca ttcatcgtgg
    45601 cacagtccag ggcggtggat tgacgaaggt gggcagcgac aacttactga tgatcaacgc
    45661 gcacattgcg cacgattgta cggtaggtaa ccgctgtatt ctcgccaaca acgcaacgct
    45721 ggcgggtcac gtatcggttg acgacttcgc gatcatcggc ggcatgaccg cagtccatca
    45781 gttctgcatc attggtgcgc acgtgatggt tggcggctgc tccggtgtgg cgcaggacgt
    45841 ccctccttat gtcattgcgc agggtaacca cgcaacgccg ttcggtgtca atatcgaagg
    45901 gctgaagcgc cgcggattca gccgtgaggc gattaccgct atccgcaatg cgtataagct
    45961 gatttatcgt agcggtaaaa cgctcgatga agtgaaaccg gaaattgctg aactggcgga
    46021 aacatatccg gaagtgaaag cctttaccga tttctttgca cgctcaacgc gcggtctgat
    46081 tcgttaatga ctgaacagcg tccattaacg attgccctgg tcgccggaga aacctccggc
    46141 gatatcctgg gggccggttt aatccgcgct ctgaaagaac atgtgcccaa cgcccgcttt
    46201 gttggtgttg ccgggccacg aatgcaggct gaaggctgcg aagcctggta cgaaatggaa
    46261 gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc tgcgtcgctt actgcatatt
    46321 cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag atgtttttgt tggtattgat
    46381 gcgcctgact tcaatattac tcttgaaggt aacctcaaaa agcagggtat caaaaccatt
    46441 cattacgtca gtccgtcagt ctgggcgtgg cgacagaaac gtgttttcaa aataggcaga
    46501 gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag cgttttatga caaatacaac
    46561 gtaccgtgcc gctttatcgg tcataccatg gctgatgcca tgccattaga tccagataaa
    46621 aatgccgccc gtgatgtgct ggggatccct cacgatgccc actgcctggc gttgctaccg
    46681 gggagccgtg gtgcagaagt tgaaatgctt agtgccgatt tcctgaaaac ggcccagctt
    46741 ttgcgccaga catatccgga tctcgaaatc gtggtgccac tggtgaatgc caaacgccgc
    46801 gagcagtttg aacgcatcaa agctgaagtc gcgccagacc tttcagttca tttgctggat
    46861 gggatgggcc gtgaggcgat ggtcgccagc gatgcggcgc tactggcgtc gggtacggca
    46921 gccttggagt gtatgctggc gaaatgcccg atggtggtgg gatatcgcat gaagcctttt
    46981 accttctggt tggcgaagcg gctggtgaaa actgattatg tctcgctgcc aaatctgctg
    47041 gcgggcagag agttagtcaa agaattattg caggaagagt gtgagccgca aaaactggct
    47101 gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc acgcgatgca cgataccttc
    47161 cgtgaactgc atcagcagat ccgctgcaat gccgatgagc aggcggcaca agccgttctg
    47221 gagttagcac aatgatcgaa tttgtttatc cgcacacgca gctggttgcg ggtgtggatg
    47281 aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc tgcggtgatc cttgacccgg
    47341 cgcgcccgat tgccgggctg aatgattcca aaaagctgag cgaaaaacgc cgtctggcgc
    47401 tctatgaaga gatcaaagag aaagcgttga gctggagtct gggccgcgcg gaaccccacg
    47461 aaatcgacga gctgaacatt cttcatgcga ccatgctggc gatgcagcgt gccgtcgctg
    47521 ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa ccgctgcccg aaattaccga
    47581 tgcctgcgat ggctgtggtg aaaggcgata gccgcgtacc ggaaatcagt gccgcgtcta
    47641 tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct ggatattgtt ttcccgcaat
    47701 atggttttgc ccaacacaaa gggtacccaa ccgcttttca tctggaaaaa ctggctgaac
    47761 acggcgcgac cgaacaccat cggcgcagct ttgggcctgt caaacgcgca ctgggacttg
    47821 cgtcctgatt cttgtgtcga gattaagtaa accggaatct gaagatgtct gaaccacgtt
    47881 tcgtacacct gcgggtgcac agcgactact cgatgatcga tggcctggcc aaaaccgcac
    47941 cgttggtaaa aaaggcggcg gcgttgggta tgccagcact ggcgatcacc gatttcacca
    48001 acctttgtgg tctggtgaag ttctacggag cgggacatgg cgcagggatt aagcctatcg
    48061 tcggggcaga ttttaacgtc cagtgcgacc tgctgggtga tgagttaacc cacctgacgg
    48121 tactggcggc gaacaatacc ggctatcaga atctgacgtt gctgatctca aaagcgtatc
    48181 agcgcgggta cggtgccgcc gggccgatca tcgatcgcga ctggcttatc gaattaaacg
    48241 aagggttgat ccttctttcc ggcggacgca tgggcgacgt cggacgcagt cttttgcgtg
    48301 gtaacagcgc gctggtagat gagtgtgtcg cgttttatga agaacacttc ccggatcgct
    48361 attttctcga gctgatccgc accggcaggc cggatgaaga aagctatctg cacgcggcgg
    48421 tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac caacgacgtg cgctttatcg
    48481 acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat ccacgactgc tttaccctcg
    48541 acgatcctaa acgcccgcgt aactattcgc cgcagcaata tatgcgtagc gaagaggaga
    48601 tgtgtgagct gtttgccgac atccccgaag cccttgccaa caccgttgag atcgccaaac
    48661 gctgtaacgt aaccgtgcgt cttggtgaat acttcctgcc gcagttcccg accggggaca
    48721 tgagcaccga agattatctg gtcaagcgtg caaaagaggg cctggaagag cgtctggcct
    48781 ttttattccc tgatgaggaa gaacgtctta agcgccgccc ggaatatgac gaacgtctgg
    48841 agactgaact tcaggttatc aaccagatgg gcttcccggg ctacttcctc atcgttatgg
    48901 aatttatcca gtggtcgaaa gataacggcg taccggtagg gccaggccgt ggctccggtg
    48961 cgggttcact ggtggcctac gcgctgaaaa tcaccgacct cgatccgctg gaatttgacc
    49021 tgctgttcga acgtttcctt aacccggaac gtgtctccat gcctgacttc gacgttgact
    49081 tctgtatgga gaaacgcgat caggttatcg agcacgtagc ggacatgtac ggtcgtgatg
    49141 cggtatcgca gatcatcacc ttcggtacaa tggcggcgaa agcggtgatc cgcgacgtag
    49201 gccgcgtgct ggggcatccg tacggctttg tcgatcgtat ctcgaaactg atcccgcccg
    49261 atccggggat gacgctggcg aaagcgtttg aagccgagcc gcagctgccg gaaatctacg
    49321 aagcggatga agaagttaag gcgctgatcg acatggcgcg caaactggaa ggggtcaccc
    49381 gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc gaccaaaatt accgattttg
    49441 cgccgcttta ctgcgatgaa gagggcaaac atccggtcac ccagtttgat aaaagcgacg
    49501 ttgaatacgc cggactggtg aagttcgact tccttggttt gcgtacgctc accatcatca
    49561 actgggcgct ggagatgatc aacaagcggc gggcgaagaa tggcgagccg ccgctggata
    49621 tcgccgcgat cccgctggat gacaagaaaa gcttcgacat gctgcaacgc tcggaaacca
    49681 cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct gatcaagcgt ctgcaacctg
    49741 actgcttcga agatatgatc gcactggtgg cgctgttccg cccaggtccg ttgcaatcag
    49801 ggatggtgga taactttatc gaccgtaaac atggtcgcga agaaatctcc tatccggacg
    49861 tacagtggca gcatgaaagc ctgaaaccgg tactggagcc aacctacggc atcatcctgt
    49921 atcaggaaca ggtcatgcag attgcgcagg tgctttctgg ttataccctc ggtggcgcgg
    49981 atatgctgcg tcgtgcgatg ggtaagaaaa agccggaaga gatggctaag cagcgttctg
    50041 tatttgctga aggtgcagaa aagaacggaa tcaacgccga actggcgatg aaaatcttcg
    50101 acctggtgga gaaattcgcg ggttacggat ttaacaaatc gcactctgcg gcctatgctt
    50161 tggtgtcata tcaaacgtta tggctgaaag cgcactatcc ggcggagttt atggcggcgg
    50221 taatgaccgc cgatatggac aacaccgaga aggtggtggg cctggtggat gagtgctggc
    50281 ggatggggct gaaaatcctg ccaccagata taaactccgg tctttaccat ttccacgtca
    50341 acgacgacgg cgaaatcgtg tatggtattg gcgccatcaa aggggtaggt gaaggtccga
    50401 ttgaggccat catcgaagcc cgtaataaag gcggctactt ccgcgaactg tttgatctct
    50461 gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct ggaaaaactg atcatgtccg
    50521 gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat gaactcgctg ggtgatgcgt
    50581 taaaagcggc agatcaacac gcgaaagcgg aagctatcgg tcaggccgat atgttcggcg
    50641 tgctggccga agagccggaa caaattgaac aatcctacgc cagctgccaa ccgtggccgg
    50701 agcaggtggt gttagatggg gaacgtgaaa cgttaggtct gtaccttacg ggacacccta
    50761 tcaaccagta tttaaaagag attgagcgtt atgtcggagg cgtaaggctg aaagacatgc
    50821 acccgacaga acgtggtaaa gtcatcacgg ctgcggggct cgttgttgcc gcgcgggtta
    50881 tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct ggatgaccgt tccgggcggc
    50941 tggaagtgat gttgtttact gacgccctgg ataaatacca gcaattgctg gaaaaagacc
    51001 gcatacttat cgtcagcgga caggtcagct ttgatgactt cagcggtggg cttaaaatga
    51061 ccgctcgcga agtgatggat attgacgaag cccgggaaaa atatgctcgc gggcttgcta
    51121 tctcgctgac ggacaggcaa attgatgacc agcttttaaa ccgactccgt cagtctctgg
    51181 aaccccaccg ctctgggaca attccagtac atctctacta tcagagggcg gatgcacgcg
    51241 cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag cgatcgttta ttaaacgatc
    51301 tccgtggcct cattggttcg gagcaggtgg aactggagtt tgactaatac aggaatacta
    51361 tgagtctgaa tttccttgat tttgaacagc cgattgcaga gctggaagcg aaaatcgatt
    51421 ctctgactgc ggttagccgt caggatgaga aactggatat taacatcgat gaagaagtgc
    51481 atcgtctgcg tgaaaaaagc gtagaactga cacgtaaaat cttcgccgat ctcggtgcat
    51541 ggcagattgc gcaactggca cgccatccac agcgtcctta taccctggat tacgttcgcc
    51601 tggcatttga tgaatttgac gaactggctg gcgaccgcgc gtatgcagac gataaagcta
    51661 tcgtcggtgg tatcgcccgt cttgacggtc gtccggtgat gatcattggt catcaaaaag
    51721 gtcgtgaaac caaagaaaaa attcgccgta actttggtat gccagcgcca gaaggttacc
    51781 gcaaagcact gcgtctgatg caaatggctg aacgctttaa gatgcctatc atcaccttta
    51841 tcgacacccc gggggcttat cctggcgtgg gcgcagaaga gcgtggtcag tctgaagcca
    51901 ttgcacgcaa cctgcgtgaa atgtctcgcc tcggcgtacc ggtagtttgt acggttatcg
    51961 gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg cgataaagtg aatatgctgc
    52021 aatacagcac ctattccgtt atctcgccgg aaggttgtgc gtccattctg tggaagagcg
    52081 ccgacaaagc gccgctggcg gctgaagcga tgggtatcat cgctccgcgt ctgaaagagc
    52141 tgaaactgat cgactccatc atcccggaac cgctgggcgg cgctcaccgt aacccggaag
    52201 cgatggcggc atcgttgaaa gcgcaactgc tggcggatct ggccgatctc gatgtgttaa
    52261 gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat gagctacggt tacgcgtaat
    52321 tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt tttatcgcca cggtttgagc
    52381 aggctatgat taaggaagga ttttccagga ggaacacatg aacatcattg ccattatggg
    52441 accgcatggc gtcttttata aagatgagcc catcaaagaa ctggagtcgg cgctggtggc
    52501 gcaaggcttt cagattatct ggccacaaaa cagcgttgat ttgctgaaat ttatcgaaca
    52561 taaccctcga atttgcggcg tgatttttga ctgggatgag tacagtctcg atttatgcag
    52621 cgatatcaat caacttaatg aatatctccc gctttatgcc ttcatcaaca cccactcgac
    52681 gatggatgtc agcgtgcagg atatgcggat ggcgctctgg ttttttgaat atgcgctggg
    52741 gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc gacgaatatc ttgataacat
    52801 tacaccgccg ttcacgaaag ccttgtttac ctacgtcaaa gagcggaagt acaccttttg
    52861 tacgccgggg catatgggcg gcaccgcata tcaaaaaagc ccggtaggct gcctgtttta
    52921 tgattttttc ggcgggaata ccctcaaggc tgatgtctct atttcggtca ccgagcttgg
    52981 ttcgttgctc gaccacaccg ggccacacct ggaagcggaa gagtacatcg cgcggacttt
    53041 tggcgcggaa cagagttata tcgttaccaa cggaacatcg acgtcgaaca aaattgtggg
    53101 tatgtacgcc gcgccatccg gcagtacgct gttgatcgac cgcaattgtc ataaatcgct
    53161 ggcgcatctg ttgatgatga acgatgtagt gccagtctgg ctgaaaccga cgcgtaatgc
    53221 gttggggatt cttggtggga tcccgcgccg tgaatttact cgcgacagca tcgaagagaa
    53281 agtcgctgcc accacgcagg cacaatggcc ggttcatgcg gtgattacca actccaccta
    53341 tgatggcttg ctctacaaca ccgactggat caaacagacg ctggatgtcc cgtcgatcca
    53401 cttcgattcc gcctgggtgc cgtacaccca ttttcatccg atctaccagg ggaaaagtgg
    53461 tatgagcggc gagcgtgttg cgggaaaagt gatcttcgaa acgcaatcga cccacaaaat
    53521 gctggcggcg ttatcgcagg cttcgctgat ccacattaaa ggcgagtatg acgaagaggc
    53581 gtttaacgaa gcctttatga tgcataccac cacctcgccc agttatccca ttgttgcttc
    53641 ggttgagacg gcggcggcga tgctgcgtgg taatccgggc aaacggctga ttaaccgttc
    53701 ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg ttgcgggaag agtctgacgg
    53761 ttggtttttc gatatctggc aaccgccgca ggtggatgaa gccgaatgct ggcccgttgc
    53821 gccaggcgaa cagtggcacg gctttaacga tgcggatgcc gatcatatgt ttctcgatcc
    53881 ggttaaagtc actattttga caccggggat ggacgaacag ggcaatatga gcgaggaggg
    53941 gatcccggcg gcgctggtgg caaaattcct cgacgaacgt gggatcgtag tagagaaaac
    54001 cggcccttat aacctgctgt ttctctttag tattggtatc gataaaacca aagcaatggg
    54061 attattgcgt gggttgacgg aatttaaacg ctcttacgat ctcaacctgc ggatcaaaaa
    54121 tatgctaccc gatctctatg ctgaagatcc cgatttctac cgcaatatgc gtattcagga
    54181 tctggcgcaa gggatccata agctgattcg taaacacgat cttcccggtt tgatgttgcg
    54241 ggcattcgat actttgccgg aaatgatcat gacgccacat caggcatggc agagacagat
    54301 taaaggtgaa gtagaaacca ttgcgctgga acaactggtc ggtagagtat cggcaaatat
    54361 gatcctgcct tatccaccgg gcgtaccgct gttgatgcca ggagaaatgc tgaccaaaga
    54421 gagccgcacg gtactcgatt ttctactgat gctttgttcc gtagggcaac attaccccgg
    54481 ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac ggcgtttacc gcgtacgagt
    54541 cctaaaaatg gcgggataac ttgccagagc ggcttccggg cgagtaacgt gctgttaata
    54601 aataaaggag acgttatgct gggtttaaaa caggttcacc atattgcgat tattgcgacg
    54661 gattatgcgg tgagcaaagc tttctactgc gatattcttg gtttcacgct gcaaagcgaa
    54721 gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg cgcttaatgg gcaatatgtg
    54781 attgagcttt tctcttttcc gttcccgccg gagcgcccca gccgaccgga agcttgcggc
    54841 ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg cagtggcgca ccttgaaagc
    54901 cataacgtga agtgtgaagc catccgtgtc gatccataca cgcaaaaacg cttcaccttc
    54961 tttaacgatc cggacgggct gccgttggaa ctgtatgagc agtaaggctt gtcatcgccg
    55021 catttgcccg gtaacgtgcc gggcattgct actgtaaaat cgcaccatca tgacactcac
    55081 gctcaataga caacttctca cctcacgcca gattctggtg gcctttagcg gcgggcttga
    55141 ctccaccgtt ctgctgcatc agttggtgca gtggcggacg gaaaatccgg gcgtcactct
    55201 gcgcgctatt catgtgcatc acggtttaag tgccaatgcc gatgcctggg ttacgcattg
    55261 cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc gaacgcgtac aacttgcgca
    55321 agaggggctg gggattgagg cccaggcgcg gcaggcgcgt tatcaggcat ttgcccgtac
    55381 cctattgccc ggcgaagtgc tggtcaccgc gcaacatctc gacgatcaat gtgaaacctt
    55441 tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt tcggctatgg cggaagtttc
    55501 ggagtttgcc ggaacacggc ttatccgccc gttgcttgcc cgcacgcggg gggaactggc
    55561 gcagtgggca ttggcgcatg gtttacgctg gattgaagac gaaagtaatc aggacgactc
    55621 atacgatcgt aactttctgc gcctgcgcgt agtgccgtta ttgcagcagc gttggccgca
    55681 ttttgcggaa gcaacggccc gcagcgccgc actttgtgct gaacaagaga gtctgttgga
    55741 tgaactgctg gcggatgatt tagcacactg ccaaacgtcg caggggacgc tgcagattgc
    55801 gccaatgctg gcgatgagtg atgcccgccg cgcggcgatt atccgtcgct ggctggcagg
    55861 gcagaatgca ccaatgcctt cccgcgacgc gctggtgcgg atctggcagg aagtggcgct
    55921 ggcgcgggaa gatgcctccc cctgtttacg cttgggcgcg tttgaaatcc gccgatatca
    55981 gtcgcaactg tggtggatta aatccgtcac cgggcaaagt gaaaccattg tgccgtggca
    56041 gacgtggcta caaccgctgg aattaccggc ggggctggga agcgtacagc ttactgcggg
    56101 aggcgatatt cgccctccgc gtgcagacga agcggtcagc gtgcgtttca aagcggcagg
    56161 attgctgcat attgtcgggc gcaacggcgg acgtaagctg aagaaaatct ggcaagagtt
    56221 aggcgtgccg ccgtggctac gtgacaccac gccactgctg ttttatggcg aaacgctgat
    56281 tgcggcggca ggggtatttg tgacgcaaga aggtgtggct gaaggtgaga atggcgtaag
    56341 ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga taagacgcat caaacgtcgc
    56401 atccggcgaa agtcaatcag gactcgctca ccaccaccgt accgatttcc gggtggctaa
    56461 agctggtaat tttatccaga cgtagctcac gggtttcgcc agcggcctcg acgaccagat
    56521 actccacatt tttgcgggag actaaatcgc tggcttttgc ctgcaatttt tcgccatctt
    56581 tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag ctcaagatta tcgtaatcat
    56641 cacaattgat tggttgatac gtatcattca ttgacataat cgctcaccag taagtttgcc
    56701 gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg cgtcgtcggc agccatttca
    56761 ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt atcctaagtc tccgctggcg
    56821 atttccgcgt accgcttgcg tattaactca caatattttt ccacatgccc tcctgtcagc
    56881 actctgactt aaccgtggat gcaagtctaa gcctacgaag ataaactctg tttcgcaagg
    56941 tgactatacc acactcattt ctgcaatatc agcgccgcaa ctgcacgtat tccgttacaa
    57001 tggcctcctg attcgaaagg agttttctta tggcgcttaa agcgacaatt tataaagcga
    57061 cggttaatgt ggccgatctc gaccgcaacc agtttctcga tgcctctctg acgctggcgc
    57121 gccacccttc agaaacccag gagcgtatga tgctgcgctt gctggcgtgg ctgaaatatg
    57181 ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga tgatgagccg gaagcgtggc
    57241 tgcataacga tcacctgggc attgatttgt ggattgagct ggggctgccg gatgagcggc
    57301 ggattaagaa agcctgcacc caggccgcag aagtggcgct gtttacctat aatagtcggg
    57361 cggcgcatat ctggtggcag caaaatcaga gcaaatgtgt gcagtttgcc aatctttccg
    57421 tctggtatct ggacgatgaa caactggcga aagtaagcgc ctttgccgat cgtaccatga
    57481 cgctgcaggc aacgattcag gatggcgtga tctggttatc ggacgataag aataatctgg
    57541 aagttaactt aaccgcctgg caacaacctt catgattgtg atttcccgac atgttgctat
    57601 tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag ggcgcgggcg ggcagcatgt
    57661 taataagacc tcaacggcca ttcatctgcg ttttgacatt cgggcgtcca gcctgccaga
    57721 gtattacaaa gagcgtctgc tcgccgccag ccatcatttg atcagcagtg atggcgtgat
    57781 tgtcattaag gcacaggaat accgcagtca ggaactgaac cgcgaagccg cgctggcccg
    57841 gctggtggca gtgattaaag atttaacaac agaacaaaaa gcccgacgac ccacgcggcc
    57901 cacccgtgca tcgaaagagc gcaggctggc atcgaaagca caaaaatcaa gcgtgaaggc
    57961 gatgcgcggc aaagtgcgca gcggtcggga ataaaaagaa ggaatggatg gtgaaaaaag
    58021 cgatagtgac agcgatggct gtaatcagcc tctttactct gatgggatgt aataatcggg
    58081 ccgaagttga tgcgctttct ccggcgcagg ctgccgaact gaaaccgatg ccgcaaagtt
    58141 ggcgcggcgt gctgccgtgt gctgattgcg aaggaatcga aacctctctg ttcctcgaaa
    58201 aagacggaac atgggtgatg aatgagcgtt atctcggtgc tcgtgaagaa ccttcctcct
    58261 tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct ggtattaacc gacagtaaag
    58321 gtgaaaagtc atattatcgg gcgaaaggcg atgagctgga gatgctcgat cgtgaaggta
    58381 atccaattga atcgcagttc aactatacgc tggaaccggc acaatccagt ttacccctga
    58441 cgccgatgac cctgcggggc atgtattttt atatggctga tgcggcgacc ttcactgatt
    58501 gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga gctggagcgt agctacctgg
    58561 ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt agaaggtcac tttacgcttg
    58621 aggctaatcc ggatactggt gcgccaacca aagtattagc gcccgatacg gcgggtaaat
    58681 tttacccgaa taaggattgc agtagtttgg ggcagtaaac ctgcttaagg cagaaacaaa
    58741 cgcaaaattg cctgatgcgc tgcgcttatc aggcctacag catataatgc aacattttga
    58801 attagcacga ttttgtaggc cggataaggc gttcacgacg catccggcat tttgtgcacg
    58861 aggctggctt tatcactgag tcagcccaac aaagcgaccc gccttgatat aaatccctgg
    58921 cttcaggtgc ccaacatact gtaactgcgt ttctggctta aacgcagaga catcgccagt
    58981 acgcatatgc atcagatcgg cagggctaat ccagcccgat tgcgccagtg taagtggatg
    59041 cccggctttg gcaaaggcat ccgtgacaaa ttccgaacaa aaccacgact ttttgtctcc
    59101 ttcgcccaca ctgcttaact gcgctttcgc caggccgctg acgcactttg gcgaaaatcc
    59161 tcggagaacg gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca
    59221 atgccgcgat agttataacc gcgatctttg attttattgg caaacgcggt gatatctgtg
    59281 gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc
    59341 gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca
    59401 cccagaaaga ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag
    59461 gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt
    59521 tcttttattg attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt
    59581 gcctcggcat ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc
    59641 gaaagtaaaa gaaagctggg gagaaacagg cggcagtacg cctttggttt atccatttta
    59701 tacaatccat gtaaaaaaag ggccctgaaa ttcaggaccc tttctggcat cagcctttaa
    59761 tctgtttcac cagatattcg acgatgtcac cagtcttaat taactgtttc tcgccgttgc
    59821 gacgatattt atattcgata tcgtcgttgt cgaggttacg gtcgcccagc acaatagtgt
    59881 gcggaatacc gatcagttcc atatcagcaa acatcacgcc cggacgctct ttgcggtcat
    59941 ccagcagcac ttcgatacct tgtgcacgca gttcgctgta cagtttctca gcaagctctt
    60001 gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc cacctggaac ggcgcgatag
    60061 cgtcaggcca tacgatgcct cgttcgtcgt agttctgctc aatcgccgca gctaccacac
    60121 gcgttacccc gataccgtag caacccatcg tcaggatttg gttacggcca tcttcaccct
    60181 gtacggaggc tttcagtgct tcggagtact tggtacccag ctggaagatg tgaccaactt
    60241 cgataccacg tttgatcagc agcgtaccct ggccatccgg gcttggatcg ccagccacca
    60301 cgttacggat atctgcaact tccggggtag cgacatcgcg atcccagttg atgccgaagt
    60361 agtgtttacc atcgatgtta gcaccagcag cgaaatcact catcgccgca acggtacggt
    60421 caatcaccac cggaatcggc atgtttaccg gacccagtga acccggaccg gctttaacca
    60481 cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct tgcaacctgc ggcagttttt
    60541 ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag cagcgcaacc agcgggaagc
    60601 tactgccttc aaccgcttta accagcagag tcttaaccgt tttctcaatc ggcagattga
    60661 actgttcaac cagttccgcg atggttttcg cgttcggcgt atcaaccagc gtcatttcct
    60721 gggtagcagc agcgcgcggt tctttcggcg cgatagcttc tgccagttca atgttcgctg
    60781 catagtcaga ggtgtcggag aagaccacat cgtcttcacc gctctgcgcc agcacctgga
    60841 attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc ttgtacggcg cggaaatcca
    60901 gccccatgcg gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca
    60961 gggattcctg agaagtatgg aaagagtaag catctttcat caggaattcg cgggaacgca
    61021 tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt ctggatctga tagaagttca
    61081 gcggcagctg tttgtaagag ctaagctcgt tacgaatcag gtcagtgata acttcttcat
    61141 gagttgggcc gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc agttccggac
    61201 cgtactgttc ccaacgacca ctctcttgcc acaaatcggc tggctgaacc accggcatcg
    61261 acacctcgat cgcaccggcg ttgttcatct cttcacgcac gatgttttcg acttttttca
    61321 gaacgcgcac gccggtcggc agccaggtat ataacccgga ggccagcttg cggatcatcc
    61381 cggcgcgcag catcagctga tggctgatca cctcggcgtc ggcaggtgtc tccttgagag
    61441 tggagagcag gtattggcta gtacgcatgt tgttacggtt ccagttggaa ggtagaacag
    61501 gctcaaggcg agcctgggac aaaaaaaagt gatttagtct accagtgcaa aagaaatgtc
    61561 aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa cctgcgtcgg
    61621 tgacgcgcca gcgaacgtta aaatcatgta gccagacggc ataggttttg cccgtttcct
    61681 cacctttacg ataggccggg cgcgggtcct gcgccagtac ttcgcggata aacagcgtta
    61741 actgcggata acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg gtaaaactca
    61801 ccgccatctc tgccgctggc gcactttgcg catagctggc actggcatcg ggaagcgatt
    61861 cggcaaaggg gagatacggt ttgatatcca ccaccggcgt accatcgacc agatccagac
    61921 tgccgagctt cagaatcacg ctgtctttat ggcaaacaac ctctttcagc tctaccagcg
    61981 acataccaat tgggttagga cggaaagtag agcgtgttgc gaaaaccccc attctggcgt
    62041 taccgccgag gcgcggtgga cgcacagtcg gacgccagcc gccttccatc gtttgatgaa
    62101 agacgaaaag gatccacaaa tggctgaacg cttccaggcc gcgaacggcg tcggcctggt
    62161 tgtagggagc aatgagatgc agttcgccgt tggcgctttt taccagacct ggctggcgcg
    62221 gaacggcgaa tttttcttta tagggcgagc gaataacgcc tatttgctcg aactggaaac
    62281 tgctcatttc gccgtaatgt taagcgcaga accgatacat acagcctgac gatagcagcc
    62341 tggcgtaccg ctggtgactt cgcagctatg cagtaatacc gcattggcct tcattttcga
    62401 ggcgttgatt tgcatccgct tacgtgcggt tggaatgctc ggcggagagt cctgattgga
    62461 ggcctggcaa gaatcgccac tgacttcacc gagatcgcgg aacggtttgc cgactaattc
    62521 ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt ttgcaggctc
    62581 cgctttcggc tggggtgcag tgctttgaac gggttcgaca ggggatctgc ttaacatgga
    62641 acagccgctt agcatgagtg ctactaaaca gatcggtaaa gcacgcatag tatttcctca
    62701 atgtatgatc aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa gacgggcaag
    62761 cgcccgtcct gaatgatatt acaaattgtg gaaacagcct aaaaattacc agcctttaac
    62821 agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact tcatcagact gataagcctg
    62881 gacgaatttc ttcacgttct cggcgtcttt gttatcttca cgcgttacga tcaggtttac
    62941 gtacggggag tctttatctt cgacaaagat accgtctttc gctggagtca ggccaatctg
    63001 gctggcatag gtggtattga taactgccag agcgatttgc gcgtcgtcca gagatcgagg
    63061 cagctgcggt gcttccagct caacaatttt cagatttttt gggttctcaa caacatcaag
    63121 aacggtcggc agcaggccaa cgccatcttt cagtttgatc aagcccactt tttgcagcag
    63181 cagcagtgaa cgaccaagat tagttggatc gtttggcacg gcaacctgcg aaccatcctg
    63241 cagttcatcc agtgatttga ttttcttgga gtaaccagca atcggataaa caaaagtgtt
    63301 gcctactgcg accagtttgt agccacgatc tttcagttgc tgatcaaggt acggtttatg
    63361 ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct tcgtttggca gaacatagtc
    63421 gttgaaggtt accagctcaa cgtccaggcc atatttgtct ttcgcaactt tctgcgcgac
    63481 ttctgctacc tgctgttcgg caccaacaat cacgccgact ttaatgtggt ttggatcttt
    63541 ttcatcctga ccgcagccta ccagtgccag tgatccaatc agggctccca ctgccgcaaa
    63601 ggttttgaat ttgaacgcca taccttattc cttcttcaat tatttatgtt gtgttgaacg
    63661 ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg aactgaatta aataaaccag
    63721 aatgaccagc aataccagta ccgtattcat caccgtcgcg ttatagccga tgtagccata
    63781 ctgatagcca atctgaccta aaccaccggc accgactgca ccacccatcg cggaatagcc
    63841 gaccagggta atcagggtga tagttgccgc attcaccaga cccggcagtg cttccggtaa
    63901 cagcacctta cggacgatct gcatcggcgt ggcacccatt gcgcgggaag cttcaattaa
    63961 cccggttggg atctccagca gagcgttctc gaccatacgg gcaataaacg gtgctgcacc
    64021 aacggttaac ggaacaatcg ctgcctgcaa accaatcgat gtaccgacaa taacgcgggt
    64081 aaacggaatc atccatacca gcaagataat gaacgggatg gaacggaaaa tgttcacaat
    64141 cgcagaaacg gtacgataca gcttcgcgtt agcaataatt tgccccgggc gcgtaacata
    64201 aagcaggacg ccgaccggca gaccaatcac aaagccaaaa aatccggata cgaaggtcat
    64261 tgccagcgtt tcccatacgc cacgaaccag caaccacatc atcggctcag acataaccca
    64321 gtacctctac ttttacatgg tgttcctgca gccaggcaat ggcggcttgc gtatcttgtt
    64381 gtgtgccgtg catttcagtc agcatgatgc cgaacttaac gccaccggcg taatccatct
    64441 gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa agcagcgggg
    64501 catcgaccga ttgaccggta aactccaggc gcaacatcgg cacgcagtca gtaaatggct
    64561 ccgcttgcag acgttcctgg taatcttccg ggatatccag atgcagggtc gactgaataa
    64621 acttctgcgc cagcggcgtt ttcggatgcg agaacacttc acttaccgtg tcctgctcga
    64681 tcagttcgcc attgctgatg accgccacgc aatcacaaat acgcttcaca acgtccattt
    64741 cgtgggtgat caacagaatc gtcaaaccca gacggcggtt aatgtctttc agcagttcga
    64801 gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac agcaatactt
    64861 tgggattgct ggctaatgca cgggcaatcg ccacacgttg tttctgccca ccggaaagat
    64921 tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat tccgtcacgc
    64981 gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc acgttgccaa
    65041 aaacagtacg cgaagagagc aggttaaaat gctggaaaat cataccaatc tggcggcgag
    65101 ctttggtcaa ctcggattct gacagcgtgg tcagttcctg gccatcgacc agcacgctac
    65161 cctcggttgg gcgctccagc aggtttacac aacgtataag cgtactctta cccgcgcctg
    65221 aggcaccgat aacgccataa atttgtccag ctggcacatg caggctgacg ttgttcaacg
    65281 cctggatggt gcgggtgccc tggtggaaca ctttggtgat attcgaaagt tttatcattg
    65341 attatttatt atcgtcatta agttagtcgt ggcatctcga atgcctgaaa cgggcaacgc
    65401 cgtcaatgaa atggatgtta aggcatccag acgtctaaat caatcaggtt tatgcgaaga
    65461 gcactttctt gcaggtcgaa acatgcgata ctagcgtccc atgccttatt aaggagctat
    65521 aaaaggtggc gaagagcgta cccgcaattt ttcttgaccg tgatggcacc attaatgtcg
    65581 atcacggcta tgtccatgag atcgacaact ttgaatttat cgacggtgtt attgacgcca
    65641 tgcgcgagct aaaaaaaatg ggctttgcgc tggtggtagt aaccaaccag tctggcattg
    65701 ctcgcggtaa atttaccgaa gcacagtttg aaacgctgac cgagtggatg gactggtcgc
    65761 tggcggaccg agatgtcgat ctggatggta tctattattg cccgcatcat ccgcagggta
    65821 gtgttgaaga gtttcgccag gtctgcgatt gccgcaaacc acatccgggg atgcttttgt
    65881 cagcacgcga ttatttgcat attgatatgg ccgcttctta tatggtgggc gataaattag
    65941 aagatatgca ggcagcggtt gcggcgaacg tgggaacaaa agtgctggtg cgtacgggta
    66001 aacctattac acctgaagca gaaaacgcgg cagattgggt gttaaatagc ctggcagacc
    66061 tgccgcaagc gataaaaaag cagcaaaaac cggcgcaatg attaaaagat gagcggttga
    66121 aataaaaatg catttttccg cttgtcttcc tgagccgact ccctataatg cgcctccatc
    66181 gacacggcgg atgtgaatca cttcacacaa acagccggtt cggttgaaga g
//
LOCUS       DAJSKJ010000004        54795 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000004 DAJSKJ010000000
VERSION     DAJSKJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.004"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..392
                     /locus_tag="ON345_000201"
                     /pseudo
     CDS             1..392
                     /locus_tag="ON345_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            389..868
                     /locus_tag="ON345_000202"
     CDS             389..868
                     /locus_tag="ON345_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR3070323.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(922..1872)
                     /gene="corA"
                     /locus_tag="ON345_000203"
     CDS             complement(922..1872)
                     /gene="corA"
                     /locus_tag="ON345_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR3070324.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2242..3006
                     /locus_tag="ON345_000204"
     CDS             2242..3006
                     /locus_tag="ON345_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR3070325.1"
                     /translation="MAHQLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3153..5315)
                     /gene="uvrD"
                     /locus_tag="ON345_000205"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3153..5315)
                     /gene="uvrD"
                     /locus_tag="ON345_000205"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR3070326.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5399..6115)
                     /gene="yigB"
                     /locus_tag="ON345_000206"
     CDS             complement(5399..6115)
                     /gene="yigB"
                     /locus_tag="ON345_000206"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR3070327.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6115..7011)
                     /gene="xerC"
                     /locus_tag="ON345_000207"
     CDS             complement(6115..7011)
                     /gene="xerC"
                     /locus_tag="ON345_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR3070328.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7008..7715)
                     /locus_tag="ON345_000208"
     CDS             complement(7008..7715)
                     /locus_tag="ON345_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR3070329.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7712..8536)
                     /gene="dapF"
                     /locus_tag="ON345_000209"
     CDS             complement(7712..8536)
                     /gene="dapF"
                     /locus_tag="ON345_000209"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR3070330.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8573..8776)
                     /locus_tag="ON345_000210"
     CDS             complement(8573..8776)
                     /locus_tag="ON345_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3070331.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9219..9539
                     /gene="cyaY"
                     /locus_tag="ON345_000211"
     CDS             9219..9539
                     /gene="cyaY"
                     /locus_tag="ON345_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR3070332.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9579..12125)
                     /gene="cyaA"
                     /locus_tag="ON345_000212"
     CDS             complement(9579..12125)
                     /gene="cyaA"
                     /locus_tag="ON345_000212"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR3070333.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            12512..13453
                     /gene="hemC"
                     /locus_tag="ON345_000213"
     CDS             12512..13453
                     /gene="hemC"
                     /locus_tag="ON345_000213"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR3070334.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTCLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            13450..14190
                     /gene="hemD"
                     /locus_tag="ON345_000214"
     CDS             13450..14190
                     /gene="hemD"
                     /locus_tag="ON345_000214"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR3070335.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            14212..15429
                     /gene="hemX"
                     /locus_tag="ON345_000215"
     CDS             14212..15429
                     /gene="hemX"
                     /locus_tag="ON345_000215"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR3070336.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            15432..16628
                     /gene="hemY"
                     /locus_tag="ON345_000216"
     CDS             15432..16628
                     /gene="hemY"
                     /locus_tag="ON345_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR3070337.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQHIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            17307..17468
                     /locus_tag="ON345_000217"
     CDS             17307..17468
                     /locus_tag="ON345_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070338.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(17552..18787)
                     /locus_tag="ON345_000218"
     CDS             complement(17552..18787)
                     /locus_tag="ON345_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR3070339.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(18934..19010)
                     /locus_tag="ON345_000219"
     tRNA            complement(18934..19010)
                     /locus_tag="ON345_000219"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18974..18976),aa:Pro,seq:tgg)
     gene            complement(19053..19139)
                     /locus_tag="ON345_000220"
     tRNA            complement(19053..19139)
                     /locus_tag="ON345_000220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19103..19105),aa:Leu,seq:cag)
     gene            complement(19160..19235)
                     /locus_tag="ON345_000221"
     tRNA            complement(19160..19235)
                     /locus_tag="ON345_000221"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19200..19202),aa:His,seq:gtg)
     gene            complement(19294..19370)
                     /locus_tag="ON345_000222"
     tRNA            complement(19294..19370)
                     /locus_tag="ON345_000222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19334..19336),aa:Arg,seq:ccg)
     gene            complement(19473..20858)
                     /locus_tag="ON345_000223"
     CDS             complement(19473..20858)
                     /locus_tag="ON345_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3070340.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(21049..21789)
                     /gene="rffM"
                     /locus_tag="ON345_000224"
     CDS             complement(21049..21789)
                     /gene="rffM"
                     /locus_tag="ON345_000224"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR3070341.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(21792..23144)
                     /gene="wzyE"
                     /locus_tag="ON345_000225"
     CDS             complement(21792..23144)
                     /gene="wzyE"
                     /locus_tag="ON345_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR3070342.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(23141..24220)
                     /gene="rffT"
                     /locus_tag="ON345_000226"
                     /gene_synonym="wecF"
     CDS             complement(23141..24220)
                     /gene="rffT"
                     /locus_tag="ON345_000226"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR3070343.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(24217..25467)
                     /gene="wzxE"
                     /locus_tag="ON345_000227"
     CDS             complement(24217..25467)
                     /gene="wzxE"
                     /locus_tag="ON345_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR3070344.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(25469..26599)
                     /gene="rffA"
                     /locus_tag="ON345_000228"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(25469..26599)
                     /gene="rffA"
                     /locus_tag="ON345_000228"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR3070345.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(26604..27278)
                     /gene="rffC"
                     /locus_tag="ON345_000229"
                     /gene_synonym="wecD"
     CDS             complement(26604..27278)
                     /gene="rffC"
                     /locus_tag="ON345_000229"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR3070346.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(27256..28137)
                     /gene="rfbA"
                     /locus_tag="ON345_000230"
     CDS             complement(27256..28137)
                     /gene="rfbA"
                     /locus_tag="ON345_000230"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR3070347.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(28156..29223)
                     /gene="rffG"
                     /locus_tag="ON345_000231"
     CDS             complement(28156..29223)
                     /gene="rffG"
                     /locus_tag="ON345_000231"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR3070348.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(29223..30485)
                     /gene="wecC"
                     /locus_tag="ON345_000232"
     CDS             complement(29223..30485)
                     /gene="wecC"
                     /locus_tag="ON345_000232"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR3070349.1"
                     /translation="MSFTTISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(30482..31612)
                     /gene="wecB"
                     /locus_tag="ON345_000233"
     CDS             complement(30482..31612)
                     /gene="wecB"
                     /locus_tag="ON345_000233"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR3070350.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHIEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(31668..32714)
                     /gene="wzzE"
                     /locus_tag="ON345_000234"
     CDS             complement(31668..32714)
                     /gene="wzzE"
                     /locus_tag="ON345_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR3070351.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(32726..33829)
                     /gene="wecA"
                     /locus_tag="ON345_000235"
     CDS             complement(32726..33829)
                     /gene="wecA"
                     /locus_tag="ON345_000235"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR3070352.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPITALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLSSIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(34069..35328)
                     /gene="rho"
                     /locus_tag="ON345_000236"
     CDS             complement(34069..35328)
                     /gene="rho"
                     /locus_tag="ON345_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR3070353.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(35413..35514)
                     /gene="rhoL"
                     /locus_tag="ON345_000237"
     CDS             complement(35413..35514)
                     /gene="rhoL"
                     /locus_tag="ON345_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR3070354.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(35655..35984)
                     /gene="trxA"
                     /locus_tag="ON345_000238"
     CDS             complement(35655..35984)
                     /gene="trxA"
                     /locus_tag="ON345_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR3070355.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            36115..37380
                     /gene="rhlB"
                     /locus_tag="ON345_000239"
     CDS             36115..37380
                     /gene="rhlB"
                     /locus_tag="ON345_000239"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR3070356.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            37514..38998
                     /gene="gppA"
                     /locus_tag="ON345_000240"
     CDS             37514..38998
                     /gene="gppA"
                     /locus_tag="ON345_000240"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR3070357.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(39045..41066)
                     /gene="rep"
                     /locus_tag="ON345_000241"
     CDS             complement(39045..41066)
                     /gene="rep"
                     /locus_tag="ON345_000241"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR3070358.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            41283..41732
                     /locus_tag="ON345_000242"
     CDS             41283..41732
                     /locus_tag="ON345_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3070359.1"
                     /translation="MAINFSPKVGEILECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            41931..42212
                     /gene="ppiC"
                     /locus_tag="ON345_000243"
     CDS             41931..42212
                     /gene="ppiC"
                     /locus_tag="ON345_000243"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR3070360.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(42299..43774)
                     /gene="ilvC"
                     /locus_tag="ON345_000244"
     CDS             complement(42299..43774)
                     /gene="ilvC"
                     /locus_tag="ON345_000244"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR3070361.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            43924..44817
                     /gene="ilvY"
                     /locus_tag="ON345_000245"
     CDS             43924..44817
                     /gene="ilvY"
                     /locus_tag="ON345_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR3070362.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNNK"
     gene            complement(44869..46413)
                     /gene="ilvA"
                     /locus_tag="ON345_000246"
     CDS             complement(44869..46413)
                     /gene="ilvA"
                     /locus_tag="ON345_000246"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR3070363.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(46416..48266)
                     /gene="ilvD"
                     /locus_tag="ON345_000247"
     CDS             complement(46416..48266)
                     /gene="ilvD"
                     /locus_tag="ON345_000247"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR3070364.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(48331..49260)
                     /gene="ilvE"
                     /locus_tag="ON345_000248"
     CDS             complement(48331..49260)
                     /gene="ilvE"
                     /locus_tag="ON345_000248"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR3070365.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(49280..49543)
                     /gene="ilvM"
                     /locus_tag="ON345_000249"
     CDS             complement(49280..49543)
                     /gene="ilvM"
                     /locus_tag="ON345_000249"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR3070366.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(49540..51186)
                     /locus_tag="ON345_000250"
     CDS             complement(49540..51186)
                     /locus_tag="ON345_000250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR3070367.1"
                     /translation="MNGAQWVVHALRAQGVSTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(51189..51239)
                     /locus_tag="ON345_000251"
     CDS             complement(51189..51239)
                     /locus_tag="ON345_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070368.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(51326..51424)
                     /gene="ilvL"
                     /locus_tag="ON345_000252"
     CDS             complement(51326..51424)
                     /gene="ilvL"
                     /locus_tag="ON345_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR3070369.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            51777..53297
                     /locus_tag="ON345_000253"
     CDS             51777..53297
                     /locus_tag="ON345_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR3070370.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLHYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEKT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(53322..53660)
                     /gene="maoP"
                     /locus_tag="ON345_000254"
     CDS             complement(53322..53660)
                     /gene="maoP"
                     /locus_tag="ON345_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR3070371.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            53779..54618
                     /gene="hdfR"
                     /locus_tag="ON345_000255"
     CDS             53779..54618
                     /gene="hdfR"
                     /locus_tag="ON345_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR3070372.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(54714..54789)
                     /locus_tag="ON345_000256"
     tRNA            complement(54714..54789)
                     /locus_tag="ON345_000256"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54754..54756),aa:Trp,seq:cca)
BASE COUNT        13470 a        14780 c        13848 g        12697 t
ORIGIN      
        1 aatgtatcga aaatggcatt aattcaactc cctcgacagg ataacaacct cttatcctgc
       61 aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac ttcgaggaca
      121 gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata tctcttttaa
      181 gttttcaaaa atacgctata ggatggagtg caacaattca ttttgtagca caggaccact
      241 tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga ttttttcgca
      301 tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc accaatttta
      361 acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa ttatattatg
      421 tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac agattttgta
      481 tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc catggactga
      541 cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc aacatcccct
      601 accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct tcactgacta
      661 taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa
      721 tgataattgc atcgtgaaaa attacttaat ggttattaat gaataccctt atcgtaacac
      781 tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata aaatagagcg
      841 agttttcaat ccgcatgact ttaaataaga aaatttttag aaataagccg gataaaaaaa
      901 tagctttatc cggcactcat attacagcca gttcttgcgc ttaaagtaca gatacggtgc
      961 caggcccgcg aggatcataa agataatcgc gcctgggtag ccgaaactcc acttcagttc
     1021 tggcataaac tcaaagttca tcccatagct ggaagcaacg agcgtcggcg gcaggaatac
     1081 cacggatacc accgagaaga ttttgatgat gcggttctgc tcgatgttga taaaacccat
     1141 tgccgcctgc atcaggaagt tcaccttctg gaacagggat tcgttatgcg gcagcaggga
     1201 ttcgatatct cgcaggattt cacgcgcctg ctccagttgc ccacccggta aacgcgcttt
     1261 acgcaccagg aagttgagcg cgcgctgggt atccatcaga cacaggcgaa ctttccagcc
     1321 gatatcttcc agttccgcca gcgtggagag cgcctcgtcg tactcatcgc cctgatgccc
     1381 ttccataatc acccggctca actgctccag gtcgctatag atattttcaa tttcatctgc
     1441 caactgttcg attttggttt cgaacagatc cagcagcaac tcgtaggcgt taccgtctac
     1501 catcgactgg ctacgggcac gcatacgata cagacgaaaa gcgggcagtt cacgctcacg
     1561 cagagtaaac agacgaccat cacggatggt aaatgccaca gtggagttac cggcgtgatc
     1621 ttccgcatct tcaaagaaga agaaggagtg aatatgcagg ccgtcgtcgt cttcaaagaa
     1681 acgtgccgat gcttcgatgt cttccagttc cgggcgggtt gccaggctct ggccaagttc
     1741 agattgtacg cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt
     1801 tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt ccagttgaaa
     1861 tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac
     1921 agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg gaaaaattga
     1981 ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag ttgatcctgg
     2041 atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt
     2101 gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca gaacaccaca
     2161 gaacaatagt tgaacttcgc gctttttggt actcgtcaag gtcgctaaca ggataaaaaa
     2221 atcccacatc caggacctat aatggcgcat cagctactca ttggtaaagg aatgatcacc
     2281 ttgaatctca aacggatttt tctcgccctc accttacttc ccttgtttgc tgttgccgct
     2341 gatgattgcg cactctcaga tccgacgctg accgtacagg cgtataccgt taatcctcaa
     2401 acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg aactttacat
     2461 gctctgctgg cggatatgaa tagtcagggt caggtgcaga tggcgatgaa cggcggcatc
     2521 tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca gaaggtggcg
     2581 ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt gttttatgtc
     2641 gcgggagata aagtcggcat cgttcgtctg gatgccttca aaaccagtaa agagattcag
     2701 tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc gcgtattcat
     2761 cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca tgggaatgcc
     2821 gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta tgccaaagcg
     2881 aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat gtatatgaaa
     2941 ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt agagcggaaa
     3001 ggttaaaacg atagttttgt gtcagatact gaagatggcg cagatgccgg atgcggcgta
     3061 aacgccctat ccggcctaca tgacgttgca atttattgaa tctgaatgat tttttaggcc
     3121 aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg ggcataagcc
     3181 gccaccagcc atttaatacc ctggccctga aatgccacct gcaaacggct atgctcaccg
     3241 ctgccttcca tattgacaat ggtgccttca ccaaacttag cgtggcgtac gcgctggccg
     3301 agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg gctgaccggg
     3361 cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcag ctcgccaata
     3421 aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc cgcgtaggtc
     3481 agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg ttcttcttcc
     3541 agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc cataccgacg
     3601 ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag ttgtaccgca
     3661 tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga taggaacgcc
     3721 tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt tgccgtcacc
     3781 agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg ctcgtacatg
     3841 gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg catatcggca
     3901 gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct ggcagcacgt
     3961 ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt taactggcga
     4021 tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg cgttggcgta
     4081 ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg cagatacgag
     4141 agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat acggtacggc
     4201 atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact gcggtagaga
     4261 atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg gttaaccaca
     4321 aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc accgtccgcg
     4381 ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag ggcgttagcg
     4441 gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaatagt ttcggcaccg
     4501 gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca gccgtagatt
     4561 gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag caggcggatc
     4621 cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt ggtaaaacgt
     4681 tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc gcgcagcagc
     4741 agctcggcga agtccaccag gcccgcgcgg tcacacgctt cctgatacgc ctgatacacc
     4801 ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg acgcaggcct
     4861 tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg cttctcgtcg
     4921 aggttcatgg ctttgatcag acgcttaagc aggcgcagct ggtcctcgct atcgaggatc
     4981 tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg gtgcgccagc
     5041 ccgtggaagg tgccgaccca catgccgccc tggctggtgc ccatcagttg cccgatacga
     5101 tgacgcatct ccgccgccgc tttgttggta aacgtcaccg ccataatcga gtatggcgag
     5161 cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt cttaccactg
     5221 cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc gcgctgtttg
     5281 tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta aaaataggtt
     5341 gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga tttgctgatt
     5401 atatcagcga ggtcagagat gccaaccggg aaatttccag atgcggcagt aaacggctgt
     5461 cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc attccgctgc
     5521 gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc tcgccgatcg
     5581 gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt gagcgcccgt
     5641 gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt tccggctggg
     5701 cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt aaggtgtcgt
     5761 gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttgatc attgctgcgt
     5821 gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct tgttcaatcg
     5881 aacgaaaacg ccagcgcgtc acgtcgtgat aaatctcggg ttccgcttcc cgtaccgcct
     5941 ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga taattttgca
     6001 caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg taaagggtat
     6061 catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa cgcattattt
     6121 cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt gaaaatcaag
     6181 atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct gcacaccacg
     6241 aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt tatgtgggtg
     6301 aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct gcacattacg
     6361 cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc taccaaacag
     6421 gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa tcggcaggcg
     6481 gcgctctttg ctgcctttcc ccataaccca cacttcacca gactccaggt cgaggtgttt
     6541 aatatccagc cccaccagct cagaaagacg cagacccgcg ccgtacatca cttccagcat
     6601 tgcacggtcg cgtacagcga ggggatcatt gatatcaata tccagcagcc gattcatatc
     6661 gtcgacgtcg atgtttttcg gcagatgacg cggcgctttc ggtgccgaaa cacctttcgc
     6721 cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac gtagcgcaga
     6781 aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta cagcaaaatt
     6841 gcgcaccatc gtcacatcac attgctgcca gctttgcagg ccgttttcgc tggcaaaatt
     6901 gatgatcgcc tcaagctgac gctggtagtt aagcagggtt atcgggctaa gctggcgctc
     6961 cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca tacgcgttca
     7021 atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa ctgcgttcct
     7081 tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacgacacc caaatcagca
     7141 tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg tagcaccacc
     7201 agcagctctg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa acgctgaata
     7261 cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga cggcgcacct
     7321 aagcgccagc gatccggaaa caggcgcaga ctcgcacctg ccaggccgag atcgcgcgcc
     7381 cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag actgcgctgc
     7441 aggtagagta ggcgataaaa caggccttcg ttggcgatag cctgttccat caacagcgcc
     7501 atgttctctt ccagaacatg aatatgatta cgtgcgcggg ccatgtgcca ctcgaccaac
     7561 gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg cgcattacgg
     7621 ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc aagctccgtg
     7681 agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatccg tcgtagacat
     7741 gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg atatcaagac
     7801 gaccgccggg gagttccacg cgtacttctt cggccagcaa accttgctga atccctactg
     7861 caaccgccgc acacgcaccg ctgccgcagg cctgggtttc tcctgcccca cgctcataaa
     7921 cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg gcgcgctccg
     7981 gaaaacgctc gtggctttcc agaacaggac caagcgtttc taccgccgcg gtatcgacat
     8041 catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat aagattgtct
     8101 gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac ggcacggcgg
     8161 aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg gtgacggtca
     8221 gaaccatccg tccgttggcg gtgctgacgc ggatatcacg cttattggtc agtcctttca
     8281 gacgcacaaa acgggcaaag cagcgcgcac cgttgccgca ctgcgccact tcactgccat
     8341 cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc tcaaccacca
     8401 gcagttggtc aaaccctacc cccaggtgcc gatcagccag gcgacgaatc agctccggtg
     8461 aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca aggccatgca
     8521 ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac ttttagtaat
     8581 tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc gtggattgcg
     8641 tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc gggaaataga
     8701 gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca gtgagtgcct
     8761 taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct ttctatcatc
     8821 gcaggagaag acgtaaaagc aagcgtttag cgactcttta tgaagtctga aaagtgagga
     8881 aatgctgcga gtataacgct tactcgtggg gatttatgtt ttgctggtag tttcgtgatc
     8941 ggcacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt gttgtctttt
     9001 ttagttccgt cagcgtgagg atgctcgctc ggtaaacctg atcataatgt ttaccagaaa
     9061 cagggaaaga cgtggtgtaa ctgcgcccta atgaagagac aaatctttcg ctacacagag
     9121 acatcattat tccggaacgc agccgcagag gtgcaaactt tgaagccctg accccatgcc
     9181 gctatactgc cgccatcact taaaaacagg atacaacaat gaacgacagt gaatttcatc
     9241 gcctggctga tcaactctgg ctgaccattg aagaacgcct ggacgactgg gatggcgaca
     9301 gcgatatcga ctgcgaaatc aacggcggcg tactgaccat tacctttgag aatggcagca
     9361 aaatcattat caaccgccag gagccgctgc accaggtatg gctggcaacc aaacagggcg
     9421 gctaccattt tgacctgaaa ggcgatgagt ggatttgtga tcgcagcggc gaaaccttct
     9481 gggatttgct ggaacaggcg gcgacacagc aagcgggtga aacagtcagt ttccgctaag
     9541 attgcatgcc ggataagcct cgctttccgg cacgctcatc acgaaaaata ctgctgtaat
     9601 agcggcgtat cgtgatcctg attggcgggc ggcatgttgc cgatagattt ggttcggaac
     9661 ggaatcacct gttcgcgacc atcaaccttc acaatctgat agaactgcgg caggttgaag
     9721 ttgatgaagc ttgagccgta ggtgaagcgg tcgtgcgatg acgagtagaa gcgactgacg
     9781 tcacgcacca gttcctcttt gctgccttcg cagtggtgat acacctcaac ccggttgctt
     9841 tcgtcgagaa tgtagatatt aaagccattc tcgtcttgcg tttcttcgaa aaagaactgg
     9901 atgatccctt cgctggcaaa gccgtccacc accgccggta atttgacgtg attggtttca
     9961 acctgcactg acaagccgtg cagtttgtta tgcgaaatag cgccataaaa ctcgatggcg
    10021 ttttccagtt tttgtaccga tacattcagg cgttcgaaga acaaccccca ggtttgacca
    10081 gaaacgcgca gcgccttgaa acgcccggtt tcctggcggg tgctggaaag gcgcagttca
    10141 atacactcag aaaccagttg ctgcacgcga gtacggatta agccgcgcag atgctggcta
    10201 taacagaaga cttccacgct atctggcggt gcggcgtcct gatgcatttt gccgagaata
    10261 gttttcaggg cttcgatcat cgattgctcg ccgttgaagt gcagcgtacg cacttcgttc
    10321 cacgagttgc ggtacagcag gtcaacgcta cctaccaggc aattttgatt ctcgccaaag
    10381 ctgaagacat ccagcttacg gaaatcaaaa tgcaccacct gattgcggaa cgccgctgtc
    10441 gggtcatatt ccaggttaac gataatcgcc agatggcgga tctcacacgg gctgtagagc
    10501 gccttcggtg tcggtgcagg taagcgcagc gggaaatggt gcgacacgtc ggcgaccatc
    10561 tcctgcaact taggcaaatc gacaatgccg ttacctttaa tatacaaacg ggtgcgcgag
    10621 gtcagcaggc cgttaaacca tgcccacgcc accagtttgt tcaggtaacg gttatattcc
    10681 agcggctgat ggctgatgat cgactcaata tttggcgcgc ggttatacag ataccaacct
    10741 gaacggttag cacggcccgg cggcacataa ataaaggtca gattcggttc cgagagatcg
    10801 ggtgaaatct gcgggtttac cagcgtcact ttacctggta atgcttcaaa cgcggcatac
    10861 agcttacgcg tcagcacgcc gatatcctgc ggactggcgg agacgctaag gttattgcga
    10921 cgcgcaaagc ggatcagatt acggtagctc tgcatcatcg cgtcgagcaa ctcgttgtgc
    10981 gcctcacgca cctgatcaat cttccagtta gcgcggttat cgagcattgc cagacgagct
    11041 tcgtcccaac cccactcgct cactaactgg ctcaacactg cgcgacgcca gcctacgcag
    11101 gcgcgttcac ggctgagctt ttcgcacact tttaaataga agcagcgacg tactaaatcc
    11161 agacgggtaa aatcttcaat cgccgtcagg tattcagtaa cacgctccag catcatgcag
    11221 tatggatcga gaccaaacga tacaatctcg ccgtcgtgca aacgctgttt gatatctttc
    11281 gccagcagac gtgggttcgg gtattcccag gaataggctt ccagcagcag tgttttcagt
    11341 accgctttgt atggggaatc gatactcttg tagagctgcc aaaggctggc accaaagtac
    11401 tcttcagcag aaagcgagct taagccaccg agatccagcc attcatttgg cgtcagcacg
    11461 ccctgcgcgt aaagcgtcat cacatagtcg tcgtaatgct cttcttcgtc gcacggcacc
    11521 atattccaca gaatacgctt accggcgaga cgcacggcgg tacgataaaa ttcgtcaagc
    11581 agcagtatat gctgggtgga gccacaatct tcgcccccca ggctgccgct ttcattatga
    11641 cggaagcggt tttcatcaat caggaagaag ctgacttcca cacccagcga ggcggcccag
    11701 ctttccagca gactacattt acgttgtagt aactggcgct cttcgctatc gagccaggat
    11761 tgatgacaga cccagatatc caggtcagag gaacaacttt gccctacgga cgaggtgctg
    11821 cccatggtgt atacaccagt aattggaagc tcacctttcg gtgggtcctg tactgacatt
    11881 ccacgataca gttcaagctc gttcaggtag tggcgttgag tttcatcagg cgtgtaaagg
    11941 caaatgcctt tgggaacgtt accatcaagg taacccggca ttagcggatg gtgatagtgc
    12001 aacaatgtcg gcagtagact gtagacctgt tggaatgcag gccccatagc agcaagcgcg
    12061 cgatccacac gcaattgatt tatggcatcc agtctctgtt tcagagtctc aatatagagg
    12121 tacaagacgt atcgcctgat ttgctacccg tcatgactgt gattccgcca acatcaacgg
    12181 taacacgcgg cattcgggat atttcgtatg tcaaaggtaa ccgttaccac ttttcgcacc
    12241 tggttttttt agtttcacga cgaaaaaatg gtctaaaacg tgatcaattt aacaccttgc
    12301 tgattgaccg taaagaaaga tgcgctacat acaagtgtag caccgtttat tctctgtaaa
    12361 ttccttatta cgacagcgtg aaacgtctgt caggatccac tgccagacct cattttacgg
    12421 tttgcgcagg cgtctacgtt tcaccacaac actgacatca ctctggcaag gatgttagga
    12481 tggaccacgg atgataatga cggtaacaag catgttagac aatgttttaa gaattgccac
    12541 acgccaaagc ccacttgcac tctggcaggc acactatgtc aaagacaagt tgatggcgag
    12601 ccatccgggc ctggtcgttg aactggtacc gatggtgacg cgcggcgatg tgattcttga
    12661 tacgccgctg gcgaaagtag gcggaaaagg cttatttgta aaagagctgg aagtcgcgct
    12721 cctggagaat cgcgccgata tcgccgtaca ttcaatgaaa gatgtgccgg ttgaattccc
    12781 gcaaggtctg gggttggtca ctatttgtga gcgtgaagat cctcgcgatg cctttgtgtc
    12841 caataattat gacagtctgg atgcgttacc ggcaggcagt atcgtcggga cgtccagttt
    12901 acgtcgccag tgccaactgg ctgaacgccg cccggatctg attatccgct ccctgcgcgg
    12961 caacgtcggc actcgcctga gcaaactaga taacggcgaa tacgatgcca tcattcttgc
    13021 cgtagccgga ctaaaacgtt taggtctgga gtcacgtatt cgcgccgcgt tgccacccga
    13081 gatttctctt ccggcggtag ggcaaggtgc ggtgggtatt gaatgccgcc ttgatgatgc
    13141 acgcactcgc gagctgcttg ccgcgctgaa tcaccacgaa actgcactgc gcgttaccgc
    13201 agaacgcgcc atgaatacct gtctcgaagg cggatgtcag gtgccaattg gtagctacgc
    13261 cgagcttatt gatggtgaaa tctggctgcg tgcgctggtc ggcgcgccgg acggttcgca
    13321 gattattcgc ggtgaacgcc gcggtgcgcc gcaaaatgcc gaacaaatgg ggatttcgct
    13381 ggcagaagag ctactgaata acggcgcgcg cgagatcctc gctgaagtct ataacggaga
    13441 cgccccggca tgagtatcct tgtcacccgc ccgtctcccg ctggagaaga gttagtgagc
    13501 cgtctgcgca cactggggca ggtggcctgg cattttccgc tgattgagtt ttctccgggt
    13561 caacaattac cgcaacttgc tgatcaactg gcagcgctgg gggagagcga tctgttgttt
    13621 gccctctcgc aacacgcggt tgcttttgcc caatcacagc tgcatcagca agatcgtaaa
    13681 tggccccgac tacctgatta tttcgccatt ggacgcacca ccgcactggc actacatacc
    13741 gtaagcggac agaagattct ctacccgcag gatcgggaaa tcagcgaagt cttgctacaa
    13801 ttacctgaat tacaaaatat tgcgggcaaa cgtgcgctga tattacgtgg caatggtggt
    13861 cgtgagctaa ttggggatac cctgacggcg cgcggtgctg aggtcacttt ttgtgaatgt
    13921 tatcaacgat gcgcaatcca ttacgatggt gcagaagaag cgatgcgctg gcaatcccgc
    13981 gaggtgacga tggtcgttgt taccagcggt gaaatgttgc agcaactctg gtcgctgatc
    14041 ccacaatggt atcgtgagca ctggttacta cactgtcgac tattggtcgt cagtgagcgt
    14101 ttggcgaaac tcgcccggga actgggctgg caagacatta aggtcgccga taacgctgac
    14161 aacgatgcgc ttttacgggc attacaataa ctctcataac aggaagccat aatgacggaa
    14221 caagaaaaaa cctccgccgt ggttgaagag accagggagg ccgtggacac cacgtcacaa
    14281 cctgtcgcaa cagaaaaaaa gagtaagaac aataccgcat tgattctcag cgcggtggct
    14341 atcgctattg ctctggcggc gggcgtcggt ttgtatggct ggggtaaaca acaggccgtc
    14401 aatcagaccg ccaccagcga tgccctggct aaccaactga cggcattgca aaaagcccag
    14461 gagagccaaa aagccgagct ggaaggcatt attaagcaac aagctgcaca acttaagcag
    14521 gcgaatcgtc agcaagaaac gctggcaaaa cagttggatg aagtccaaca aaaggtcgcc
    14581 accatttccg gcagcgatgc taaaacctgg ctgctggctc aggccgattt tctggtgaaa
    14641 ctcgccggac ggaagctgtg gagcgatcag gacgtcacga ccgctgcagc gttgctgaaa
    14701 agtgcagatg ccagcctggc ggatatgaat gacccgagtc tgattaccgt tcgtcgggca
    14761 attaccgatg atatcgccag cctttctgca gtttcgcagg tggattatga cggcatcatc
    14821 cttaagctta atcagctttc aaatcaggta gataacctgc gtctggccga taatgacagc
    14881 gatggttcgc cgatggattc cgacggtgaa gagctttcca gttccatcag cgaatggcgt
    14941 atcaatctgc aaaaaagctg gcagaacttt atggacaact tcattacgat tcgccgtcgt
    15001 gatgacaccg ccgtaccgct gttagcgcca aatcaggata tctatctgcg cgaaaatatt
    15061 cgctctcgcc tgctggtcgc agcacaagct gtaccgcgtc accaggaaga gacttatcgc
    15121 caggcgctgg agaacgtctc cacctgggta cgtgcttact acgatactga tgatgccacc
    15181 accaaagcgt tcctcgacga ggtggaccag ttaagccagc aaaatatctc gatggatctt
    15241 ccggaaaccc tgcaaagcca ggcgatgctg gaaaaactga tgcagacccg cgtgcgtaac
    15301 ctgctggcac aaccggcagc ggggacaacg gaagctaaac ctgcacctgc acctgcacct
    15361 gcacctgcac ctgcacctgc acctgcaccg caaggtgata ctccggcagc cgcgccgcaa
    15421 ggagaataac catgctaaaa gtgttattac tctttgtttt gctgattgcg gggatagtgg
    15481 ttggcccgat gattgccggt catcagggtt acgtgctgat ccagactgac aactacaata
    15541 tcgaaaccag cgtcacgggc ctggcgatta ttttgattct ggcgatggta gtgctgtttg
    15601 ccattgagtg gctactgcgg cggatcttcc gcacaggcgc acacacccgt gggtggtttg
    15661 tcggacgtaa gcgtcgccgt gcccgtaagc agaccgaaca ggcgctgctg aaactggcgg
    15721 aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac gcggaacaac
    15781 cggtggtgaa ctatctactg gctgccgaag ccgcgcaaca acgtggtgat gaagcacgcg
    15841 ccaaccaaca tctggaacgc gcagcagagc tggccggcaa tgacaccatt ccggtagaaa
    15901 tcacccgtgt acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc cacggcgtgg
    15961 ataagctgct ggaagttacg ccacgccatc cggaagtatt acgtctggcg gaacaggcgt
    16021 atatccgcac aggtgcatgg agttctctgc tggatatcat cccatcaatg gcgaaagccc
    16081 atgttggtga tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt ggcctgatgg
    16141 atcaggcgcg tgccgataac ggtagtgaag gtttgcgtaa ctggtggaaa aaccaaagcc
    16201 ggaaaacgcg tcatcaggta gcgttgcagg tggcaatggc ggaacatctt attgaatgtg
    16261 acgatcatga tactgcccag caaattatca tcgatggcct gaaacgccag tacgacgatc
    16321 gcctactgct gccgattcct cgactgaaaa ccaacaatcc ggaacagctg gaaaaagtgc
    16381 tgcgccagca tatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca ctgggccagt
    16441 cactgatgaa gcacggagaa tggcaggaag catcgctcgc cttccgcgcg gcgctgaaac
    16501 aacgtccgga cgcctacgat tacgcatggc ttgccgacgc gctggacaga ctgcacaagc
    16561 cggaagaagc tgcagcgatg cgtcgcgacg gtttgatgtt aacgttgcag aacaacccac
    16621 cacagtagtt ccttctcacc cggaggcaag cacctccggg acctttctga tgcataaaaa
    16681 aacgcctgct cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa caagtgggtg
    16741 cttcactcaa cgttgtgtcc atggtgtctg atgaggcata agcgacatct gtcagtggac
    16801 gataagcacc gtaaacggct ctgcgtcatt ccggagttta tgaggcacta aggcgaacat
    16861 aagagatgga atgagcatct acccgtttat tatgccacag agaatcggga aataacatcc
    16921 cttaacactt gttatgagat aattctgtaa tcctctttgc ttcctgagta ataacttcct
    16981 gagtgaatat ttaacctgag cttgatccta cacatactta ttatgaatga taaaattcat
    17041 tcaattaata acacatatat taattgccgt taaaactaaa aaaagcatca ataatcaacg
    17101 cgatataata aacctgcctt acatatcaac tgcgccagag gtaggattga aaacgctctc
    17161 ctgattttcc aattcatttt ctggataaat aaataattta tttttgtcac tattatttat
    17221 gtaatcatcc tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt
    17281 gatgacatga aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg
    17341 ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc agataccccg tcaactgcca
    17401 ccgcacgcaa aggctttgcc ggatacgatc acccaaacca gtatccacag aacgctgcaa
    17461 tttattgaat ttgcacgatc atgtaggccg gataaggcgt ttacgccgca tccggcaatc
    17521 aaccgcaggc ggccacaaaa tggccgcttt tttacttatt caccaccagc aaatgtgcct
    17581 gcataatgtc actggccggg cgaccgtgcg ccagcaaatc agccattgct ttaagatatg
    17641 gcggtagatg gcggaaataa cgctgatacc cggcacacaa ataattcagc cccggtttgc
    17701 cgctggcatc gagcatgaag cggtgtttcg ggcagcctcc ccagcacgct tttaacacgt
    17761 tacaactgcg acactgcgcc ggtaattgct taaatttatc ttcaccaaac acctgctgtt
    17821 gcggggaatc gatcatttct gcaattgttt gctgatgcat attccccagc cgatattgcg
    17881 gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc accgagcgcc
    17941 cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg gcaaacgccc
    18001 attcgatatt catcacgaaa atcttgccga cgtcgcgttt gatccagtgg tcgaatatcg
    18061 ccaccagaaa ttcaccaaat tcatcggggc gcaccgacca ttccgtcagt tccccctgaa
    18121 tatcaccagg cgcatgtagt ttcagtccat cgctggcagc tgtttcatca gccaggcgct
    18181 cgaccaccgg aataaactgg atgaattcga ctcccgcatc gcacaaaaaa tcataaacct
    18241 gcaacggttg ctgcgcgctg gtgcggttga cgcagaccag cacgttatag tcgacatgat
    18301 gtttttgcag cagcgtcagg gcacgcatca ccagcttatg cgttggtctg ccacctttgg
    18361 tcacgcgata ttgattgtgg atctcagccg gaccgtccag cgataaccca acaagaaaat
    18421 gattttctgc cagaaatgca caccatttat catcgagcag cacgccgtta gtctggaagc
    18481 tgttacttat cttcctgcca gcaccgtatt tcgcctgtaa ctttacggca cggcggtaaa
    18541 aatccagccc gagtaacgtt ggttcgccgc cctgccaggt aaaagccact tcgttttgcg
    18601 gttcgctggc agcgatatag tgacggacat acgcttccag cgtgtcatcg tccatatgcg
    18661 tgactggctt ttcgcggtaa agggattgtt tttcgagata aaaacagtag tcacagttca
    18721 gattacaatc ggaaccactc ggtttcgcca tcacatgaaa agcacgcgtt ggaacctgtt
    18781 gcagcatggt cactccttgc ctgaagtcat ccttcaatac gttaagggcg aaatcatacc
    18841 gtggggcaat gagttattac gggcgtttat tgcgaaggga gggtgacgaa atgcacagaa
    18901 aacaaaaaac cccgccgaag cggggttcaa aattggtcgg cgagagagga ttcgaacctc
    18961 cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg aaatactgct
    19021 ttttgaattt ttagttcaat tctttaaagt cgtggtgcga ggggggggac ttgaaccccc
    19081 acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc caccttcgca
    19141 ttgtcacaac ttctaataat ggggtggcta atgggattcg aacccacgac aactggaatc
    19201 acaatccagg gctctaccaa ctgagctata gccaccacta caaatcttgt tacgcggtat
    19261 tactaccacc gcagctcaag cgccgggact aaatggcgcg cccgacagga ttcgaacctg
    19321 agacctctgc ctccggaggg cagcgctcta tccagctgag ctacgggcgc ttagcgccgt
    19381 tgcgggggtg gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg
    19441 cgcgtttggt tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttcccg
    19501 tggcgattaa ggccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgcca
    19561 acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg
    19621 aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca
    19681 atcgccgctt tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc
    19741 accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga
    19801 atgatgtagt tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca
    19861 actggtacgc cgtgacggga aactttcgcc atcgccgccg gtaactgacg gtttttcgcc
    19921 agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc
    19981 gtcagcacca caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc
    20041 agtacgaacg ggctgccgtt gctgccaatt tcattccacg ggaagatggt gacgataacg
    20101 aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca ctttgcctac ggcactgcgc
    20161 agcgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg
    20221 taggacgcca ccacaataca cagcgcggtc aggaaccctt tccagccacc cgcaaagaaa
    20281 ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag
    20341 aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg
    20401 aaccagaact cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc
    20461 gccactgcga tcaatgcggg tatccactgc gccatctccg ggaaccagaa ctggacataa
    20521 acgccaatgg cggtgatttc tgagatcccc acggccatcc acataaacca gtaagaccag
    20581 gcggtgagat aaccaaagaa cgggctcata taacgatgcg cataaacggc gaacgaaccg
    20641 gtaaccggtt cgaggaacaa catttcgccc attgaacgca tgatgaaaaa gacgaatagc
    20701 ccggcgatga tataggccaa caatacggat ggcccggccc atttcaaggt actggcggcc
    20761 cccataaaca ggccgacgcc aatggtgccc cccagggcga tgagttcgat atgtcgagct
    20821 tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca
    20881 tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta
    20941 attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc
    21001 agacaggcga cggagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc
    21061 ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg
    21121 cgaaagcagg cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt
    21181 tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg
    21241 caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac
    21301 gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa
    21361 ataaccatcc tgactgccaa cgatattcac attccactgg ttgcgcagtt tagcttcagt
    21421 ttgcgccagc acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc
    21481 gcgcgccatc agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg
    21541 gtacttttta cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc
    21601 gttaattaac tccctgacct cggcgttatc ttcaatggtc agcatttttt cagcattaat
    21661 ggcaaccagc gttccctgct taagctgccc gtcagcaaac agataatcga gggcgtgctg
    21721 catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt
    21781 gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaata
    21841 agtccggcgc tttcaaaaag ccagtacaac agttttgcga tcatcagaca tgcgccgaag
    21901 accacgataa aaaagactac gcgtgagaca aacgaatcca gcccttcacg tgccagcacg
    21961 atcatattga agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta
    22021 ggctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag accaaccacg
    22081 atcgccccga gcgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta
    22141 ggcgagatcg ccagtccgga gtggttattc agcacttccc aggtaaagta gttggctgag
    22201 ttcagcacca tactcgggcg acccggccac agccaggaag ggataaagac atagaaatcg
    22261 cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc
    22321 agattctccc acggcgagaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca
    22381 tcgccgctca cattcattcc atagcgtttt agcgccagcc agaacatgcc cacaatcccc
    22441 agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac
    22501 aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc
    22561 agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc acgctttgct atcctggcgc
    22621 agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg
    22681 ccggagactt cactggagaa gatctgactg taggagttaa gccggaacag caaaaagccg
    22741 ttgtgcatga agaagatgcc gacgcttacc agcgcgatgc ccatcaggat cacccacgta
    22801 agattggtct ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt
    22861 ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc
    22921 aacgcctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg
    22981 ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg
    23041 aagttaaagc gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag
    23101 agcagccaga caacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg
    23161 caatcgccaa cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt
    23221 ttttatccac cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt
    23281 cggtagtaaa cagcaccggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc
    23341 ggttaagcac acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct
    23401 ggcgggcaaa aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa
    23461 attccagttt ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct
    23521 gacgaacttc ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt
    23581 ttaccgtatc gccaaattgc tgatgaacgg cgcacaaggc agcaatatgg tcattgctgc
    23641 ggtcaccgga gttccccacc agaatggtca ttttcccttc acgttgccga tcgttcgcca
    23701 tcgtattgag agaagggtcc atccgcgtcg ggaagtacag cagttcgccc cgcacctttg
    23761 gatgcgtttt ggcaaaaaag ctcaaatcgc cgcgggtggc aaatacacag ccgactcgct
    23821 tttgcgccag gcgacgtagt gggtaaaaaa gcttatatct caagccactg gaaagctcat
    23881 acaggtctgc cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca
    23941 gagccagcca cagtgtggga ttgaactgac cgtggaagaa aaaacgctgc tgacggttag
    24001 cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag
    24061 aaagcgccgg acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg
    24121 cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt
    24181 gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa
    24241 aaacacgcca caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc
    24301 gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg
    24361 gctgacttcc gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa
    24421 gacataagcg cccactttta acacatcacc cactaactgc caggcaaaga gatcgcgcat
    24481 agcggtaaat ttattcgaca acagcagcca gatagcaaaa tcacgcagca gccagacggt
    24541 aaaactcgcc gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt
    24601 gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc
    24661 cgtaataaat tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc
    24721 atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt
    24781 caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc
    24841 ccagctgggt ttcagatagc ttaacgggat gacaccacgt ttgatcaaca tgatggcggc
    24901 aggaattacc accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc
    24961 caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa
    25021 cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc
    25081 gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa
    25141 caatccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa
    25201 accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg
    25261 gtactgggca acgtatttgg ttacaccgtt aaagatgcca gctccggcaa gcacgccgag
    25321 cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac
    25381 agccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt
    25441 ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta
    25501 ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc
    25561 tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc
    25621 aaccgtgcag cggaatgtaa tggaacaccg ccatgatttc cgcttctttc agaaagttaa
    25681 tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca
    25741 cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg
    25801 catcgtagta gttttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca
    25861 gttgcgccca caggtacgca gcttgcagat cggacatcaa atagctggag ccaatatcgc
    25921 gccaggtata tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac
    25981 ggatgatctc ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac
    26041 cacccgccgt atagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc
    26101 ccagtgcacg ccctttgtaa gtggacatca cgccctgagc ggcatcttct accacaaaca
    26161 gattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat
    26221 ggaccggcac gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc gtttcgtcga
    26281 tgttcatggt gtccgggcga acatccacaa aaacgatttt tgcgccacgc agcacaaagg
    26341 cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat
    26401 cgagcagcag cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg
    26461 cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac
    26521 acagtttgcc gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg
    26581 gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc
    26641 gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc
    26701 aaagttgttt taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct
    26761 acaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag
    26821 acataaccgc gaataccgcc ggacgccgca cgtaaaatca gacattgatg atcaaaggtg
    26881 ccgcgcacgg cattttcaat ccactgtgca taaaagcgtc tgctggcgtc aggcgcatac
    26941 cacggcgcac gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga
    27001 atatcggtct cttgtgccac tacagcacca ctatcactga cattgttcac gggtagtgcc
    27061 aaatcaactt caccttctac cagggagaat cccagctgtt gcagggcatc cagttcaccc
    27121 gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacac gtctggcgtc
    27181 aggagcggtg cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt
    27241 tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg
    27301 gccgtagcca gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca
    27361 gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac
    27421 tgtctgcaca aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc
    27481 aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat
    27541 ggaggtaatc tccagttcac cacgctccga cggcttcacc tgctttgcgt actccacgac
    27601 tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt
    27661 ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc accacgccaa agcgttccgg
    27721 gtccatcacc tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa catgacgcag
    27781 cttcggactg aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc
    27841 accgttgagg aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg
    27901 ttcggcgtat tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata
    27961 acctttatct tccggcgtag tgatgatgag aatttcgcgg ataccggcca acatcagcac
    28021 cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc
    28081 gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc aggataatac ctttcatgtt
    28141 tgcctccgct gaaaattagc ctttcagacc taaacgctcg ccctgatagc tgccgtcctg
    28201 cacctgcttc caccagcttt cattagccag ataccactgc accgttttac gcattccact
    28261 ttcaaaggtt tcctgtggca gccaaccaag ttcacgggca attttcgaag catcaatggc
    28321 ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaagtcac gatagtgcgc
    28381 cacgccgtgc ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg tttccacaac
    28441 atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt
    28501 ggtcgccacg caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg
    28561 ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg
    28621 gatcagtttt tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgatcagcgt
    28681 cggcagaccg taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc
    28741 ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg
    28801 caggtcacca tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc
    28861 ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat
    28921 gttggtttca ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc
    28981 cagatgcatg acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc
    29041 gcagatatca actttctcaa aggcaaagtg ctcgctttgc gcgaccggtg ccagcgacat
    29101 caggtttccg gcgtaggtca gcttatcgac cactaccacc gcatcgctcg tttcgttgat
    29161 gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct
    29221 catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg
    29281 ataactttga actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc
    29341 tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta
    29401 ggctcaacaa ccagcgtttc gccgctgtgc cactgggcga tcagttcagc gatttccatc
    29461 gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt
    29521 ttcagttcac tggcgcgttt atcggtagca gcaaggcagt cggcaaccgc cgctttcacc
    29581 tgatcgataa cccagaacgg tttgtgatcg ttaacttcgc gcgcggtacg gataagccgc
    29641 gcctgctggg ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc
    29701 acgccagggc caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt
    29761 tcccagacgt taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata
    29821 ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg
    29881 acgacacact caccttcgag gaaaattttg tacagttcgc tggcgcgggt cgaacaaacc
    29941 ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt
    30001 aacacgcgtt ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg
    30061 aaagtgagat ccggacgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc
    30121 ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga
    30181 gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtgggtaca
    30241 gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct
    30301 acggcagttt ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg
    30361 atggtatcaa ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca
    30421 aacgctgctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgtaaaa
    30481 ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat
    30541 caccatacgg gttatgggcg cggctcatag tttgatattc gttttcgtct tttaaaagac
    30601 gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg
    30661 tcaccgcttc cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag
    30721 gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc
    30781 agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa
    30841 tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt
    30901 cctggtgcgt ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag ccacgaccga
    30961 aactttcacg cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg ataaacgggt
    31021 aatttgccgc cagttctgaa cgcagcgtgt cgctgctcat cacctgatca cgcacccata
    31081 acagtgcatc aatgactgta ttaccagtaa tgaagattcg gctatccgca acgttttcac
    31141 gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg
    31201 tcaatgtacg gttagcctct tccggccacg gcgaatagag atcgcccgtg cgtaaaccag
    31261 cctcaatgtg accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg
    31321 ttgttgtgtc gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta
    31381 gcccttccag aatccgacaa gttatctctg tcaggccctg tcctggctgc attatgttga
    31441 gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg atcgagcatc tcccgatgct
    31501 gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg
    31561 gagccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc
    31621 tcttcgaata agcggcgagc gcctttgcgc tcgccgcagc agtgttgcta tttcgagcaa
    31681 cggcgggtta atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc
    31741 aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga
    31801 taggtctgaa aacgcggatc cagggttgga ccaacattca gggtgtttaa catggcccga
    31861 ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc
    31921 tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca
    31981 tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ctcaatgctg
    32041 ttcatccggc ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgagctttc
    32101 atctggatgg tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca
    32161 cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttcg
    32221 gcaataagct tcacgctgtc attgaccgcg cgggtaaagt ctccggggat aaactggatg
    32281 ttgttaatca tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt
    32341 ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata
    32401 acaaactctt tataggcttc gtccatgacc gatggttgat cggcagaagc catgtttgaa
    32461 cggacatcca ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc
    32521 gttggacgat cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc
    32581 agcgcgatta acgcaaacgc cagccccatg ccaataatcc atagcttccc agcccacaag
    32641 gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc
    32701 attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc
    32761 tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc
    32821 aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa
    32881 aatgagaata ttctgccagc acgccaatgg aagagagcag tgctgcggca agggtaatca
    32941 gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac
    33001 ggtcaggaga gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat
    33061 ccattagcgg aatggcgatt atccacaaag cggtaatcgg gctgatggga tgggttttgc
    33121 cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat
    33181 cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca
    33241 ggatggcggc gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca
    33301 aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc
    33361 catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac
    33421 caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa
    33481 gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta
    33541 cgctgatatc aaaacggtca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga
    33601 gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg
    33661 cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg
    33721 gtttatccac taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg
    33781 tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc
    33841 tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc
    33901 gttccgaacc caccagtcct gtgcaaaaat taaccatttg atatgcaaat tgcataacat
    33961 gccagcaaat tccaggaatt ttcctaacat cgccagcgcg gcattaagat tacagatata
    34021 aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt
    34081 catcatttcg aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc
    34141 cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag
    34201 ttcttcctga gtagtgagca gttcttcttt acgggtaccg gaacggttgt agtcgatagc
    34261 cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc
    34321 tttaaactct tcgtaaataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc
    34381 gataatggtc aggctgccgc cctcttccac gttacgcgcc gcaccgaaga agcgtttcgg
    34441 acgatgaagg gcattggcgt ccacaccacc ggtcaacact ttacctgacg ccggaacaac
    34501 agtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt
    34561 gtgctcaacc aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga
    34621 tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt
    34681 tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta
    34741 agcaatgctc tgagcaatgt tctgcagcag catagtttta ccggctttcg gcggtgccac
    34801 aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa
    34861 atcttcagta gaaccgttac cacgttccat acgcagacga gagtttgcgt gcagcggggt
    34921 taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt
    34981 aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat
    35041 ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc
    35101 aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat
    35161 ctccagtacg ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc
    35221 aaaaataatg tcctgcttac gcatacgagc caggttttcc agccccatat tttcgccgag
    35281 agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt
    35341 tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat
    35401 gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga
    35461 actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga
    35521 taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa
    35581 gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg
    35641 aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac
    35701 agtgcaccca ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata
    35761 ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg
    35821 gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac
    35881 ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc
    35941 gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga
    36001 ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt
    36061 atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc
    36121 aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa
    36181 gcccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg
    36241 acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg
    36301 tttcttacgt caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg
    36361 aatcaaccac gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc
    36421 gacgcagaac cgctggcgga agctaccggc ctgaagctgg gtctggctta cggtggtgat
    36481 ggctacgaca aacagctgaa agtgctggaa agcggcgttg acattctgat tggcactacg
    36541 gggcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtggtg
    36601 gtactggacg aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg
    36661 ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg
    36721 taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa
    36781 ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa
    36841 aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc
    36901 gccaatacca aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt
    36961 gtcggtttgt tgacaggcga tgtcgcacag aaaaaacgtc tgcgtattct tgatgaattt
    37021 acccgtggcg atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt
    37081 ccggcagtga cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac
    37141 cgtattggtc gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa
    37201 gagtatgcat tgaatttgcc tgctattgag acctatattg gtcactcaat tccggtaagc
    37261 aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca
    37321 cgtacaggca atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa
    37381 ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg
    37441 atgagcatac tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg
    37501 aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa
    37561 tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat
    37621 aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc
    37681 aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc
    37741 ctcgcaaatt cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgattt
    37801 tattgccaaa gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga
    37861 agcacgtctg atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt
    37921 ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc
    37981 gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa
    38041 tctggggcag gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt
    38101 tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca
    38161 ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt
    38221 gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg
    38281 gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt
    38341 taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt
    38401 ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat
    38461 tcagcgccgc tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa
    38521 cttcttcgat caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat
    38581 cagcgcctgt cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca
    38641 cgctgcttat ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt
    38701 gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca
    38761 aaatgccgta ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat
    38821 ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga
    38881 actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat
    38941 tattgctcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg
    39001 agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt
    39061 gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg
    39121 gcgctgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa
    39181 aagcggctcg gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta
    39241 aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg
    39301 atattatctt catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg
    39361 tagacataag gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc
    39421 agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc
    39481 tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta
    39541 aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg
    39601 tacagccagg attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc
    39661 cgctccgcca gacgctggat ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa
    39721 ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta
    39781 ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctctcttc
    39841 ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga
    39901 taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc agatattttg
    39961 tatgggatgc ggttttgcat caggaatttt tcaaacaccc gcgactgatg gttaccgcga
    40021 taaagaatgg cgtaatcttt gtactgcgtt ttattgacga agtgatgggc gatcagctcg
    40081 ccagtaacgc gctcggcttc atgttcctcg ttattcgcgc ttaaaacttt tagctctgtg
    40141 ccgtaaccca gttcggagaa cagacgcttt tcaaagacgt gcggattatt ggcgatcagg
    40201 atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc
    40261 ttcagcgccg gaaaatcctg gctcagcagc accaggtttt gcggacgtgc gccgcgccag
    40321 gagtagatcg actggtcatc gtcacccacc acggtaaagc gcgcgcggct gcccaccagc
    40381 agtttcacca gttcatactg gctggtgttg gtgtcctgat attcgtccac cagcaggtag
    40441 cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcagaag tagcgtcggc
    40501 aataaaatca gatcatcgaa gtcgagcaca ttacaggctt tcaggtgtgc atcatacagc
    40561 ccataacaat gggcaaaaat acggtcccgc tcgccaatcg cacttgctgc cgcctgggac
    40621 ggtgttttga gatcattctt ccagttagag atggtcgaaa tcagttgttg caggagaact
    40681 ttgtcatctt caatcagccc ctcggtcaac tctttaagca aagcaagctg atcggtatcg
    40741 tcaaacagcg aaaagttcgc cttcatccca agcgccgcat actcgcgttt gatgatatcc
    40801 aaccccagcg tgtgaaaagt ggagatcatc agcccgcgcg cctctttgcg acccagcgtc
    40861 tgcccgacac gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata
    40921 tgtcgcgcct gataaccgca accgcggatc agatgggcga ttttattggt gatcacacga
    40981 gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct
    41041 tgttgttggc cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga
    41101 ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc gctaaccggt tggctgcgga
    41161 tgtttaaatc agagagagaa gaccgaaatc ggagttcatc gtgaatccat tagacttaga
    41221 aaatatcagg tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtttac
    41281 tcgtggcgat taatttcagc cctaaggttg gtgaaatact ggaatgcaac tttggtaact
    41341 atcctgttat ccagaatgga cagttcagca ccacgtatta cgatggccgc attcctcctg
    41401 aaatgataaa aaacaggtta gttgtcgtgc tcaacggaaa aattaatggc aacgcatgta
    41461 tcgtcgttcc attatcaacc actcgtgatc atgacaagtt aaatcgtgga atgcatgttg
    41521 aaatagccag taatgttatc aatgacctgc aattctttga tcagcagatt cgttgggcca
    41581 aagccgacct ggtacaacaa gtcagtcgaa accgattaaa cagagcaagg acttacagag
    41641 ggtatcttaa tcaatgtttg ccacatgagt tggttgcaga catacagagg gcggtcatca
    41701 agtcaatcaa tgccatatcg ctcataaatt gacaataaaa tcaccatccc cgataatgac
    41761 tctgtagccc atagaggccc taagttttct ccccttgagg gacctgcgaa gcccggagtt
    41821 tccgggcttt tgccatatat gtaccagccc ctccctgact gacaattacg ttagcatctg
    41881 cggatcatgc taaaatcgcc gcctgacaat tattcactca aggcacgatc atggcaaaaa
    41941 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgagc
    42001 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactctatt tgcccgtcag
    42061 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    42121 aagtggtttt ctcttgtccg gtactggagc cgaccggccc gctgcacacc cagttcggat
    42181 atcacatcat taaggtgctg taccgcaact aatagcaagg ccttctccag gagaaggcct
    42241 tgagtgtttt ctccctctcc ctgtgggaga gggtcggggt gagggcatca gcgcgcactt
    42301 aacccgcaac agcaatacgt ttcatatctg tcatatagcc gcgcagtttt ttacctacct
    42361 gctcaatcgc atggctgcga atcgcttcgt tcacatcacg cagttgcgcg ttatctaccg
    42421 cgccttccgg aatagcttta cccaagtcgc ccggttgcag ctctgccata aacggtttca
    42481 gcaacggcac acaagcgtaa gagaacagat agttaccgta ctcagcggta tcagagataa
    42541 ccacgttcat ttcgtacaga cgcttacggg cgatggtatt ggcaatcagc ggcagttcgt
    42601 gcagtgattc atagtaagca gactcttcga tgatgccgga atcgaccatg gtttcaaacg
    42661 ccagctcaac gcccgctttc accatcgcga tcatcagtac gcctttatcg aagtactcct
    42721 gctcgccgat tttaccttca tactgcggcg cggtttcgaa cgcggttttg ccggtctctt
    42781 cacgccaggt cagcagtttc ttatcatcgt tggcccagtc cgccatcata ccggaagaga
    42841 attcgccgga gatgatgtcg tccatatgtt tctggaacag cggtgccatg atctctttca
    42901 gctgttcaga aagcgcataa gcacgcagtt tcgccgggtt agagagacgg tccatcatca
    42961 gggtgatgcc gccctgcttc agcgcttcgg tgatggtttc ccaaccgaac tgaattagtt
    43021 tttctgcgta tgccggatcg gtgccctctt ccaccagctt gtcgaagcac agcagcgagc
    43081 cagcctgcaa cataccgcac aggatggttt gctcacccat caggtcagat ttcacttccg
    43141 caacgaagga cgattccagc acgcccgcac ggtgaccacc ggttgctgcc gcccatgctt
    43201 tagcgatcgc cataccttcg cctttcggat cgttttccgg gtgaacggca atcagcgtcg
    43261 gtacgccgaa tccacgtttg tactcttcac gcacttcggt gccagggcat ttcggcgcaa
    43321 ccatgactac ggtgatatct ttacggatct gctcgcccac ttcgacgatg ttgaaaccgt
    43381 gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg tacggtgcgc actacatcag
    43441 agtgctgctt gtccggcgtc aggttaacca ccagatccgc ctgcgggatc agttcttcgt
    43501 aagtaccaac tttaaaaccg ttttcggtcg ctttacgcca ggacgcgcgc ttctcggcaa
    43561 tcgcttcttt acgcagagcg taggagatat cgagaccaga atcacgcatg ttcaggccct
    43621 ggttcagccc ctgtgcgcca cagccgacga taaccacttt tttaccctga aggtagctcg
    43681 cgccgtcggc gaattcgtcg cgccccataa agcgacattt gcccagctgt gccagctgct
    43741 ggcgcaggtt cagtgtatta aagtagttag ccatggtgat tcctcgtgat gttgtgcttc
    43801 ttattgttcg gttcgcgttt gcgagattga attcactata tgacaggaaa tttattgcgg
    43861 aaattgatat attcacaacg tcacattgca atttctgcaa cgtcaacatt gagggctgcg
    43921 ccattggatt tacgcgatct gaaaaccttc ctgcatctgg cggaaagccg ccattttggc
    43981 cgcagcgcgc gggcgatgca cgttagccca tccacgctct cacggcagat tcagcgcctg
    44041 gaagaagatc ttggtcagcc gctatttgtg cgcgataacc gcacggtaac gctgaccgaa
    44101 gcgggcgaag agttacgcgt tttcgcccag caaacattgt tgcagtatca gcaactgcgc
    44161 cacaccatcg atcagcaagg gccgtcgctc tctggcgagt tacatatttt ctgctcggtg
    44221 accgccgctt acagccatct accgccgatt ctcgaccgct tccgcgcgga acacccgtcg
    44281 gtggagatta aacttactac tggtgatgcg gcagatgcga tggaaaaggt ggtcactggt
    44341 gaagcggatc tggcgattgc gggtaaaccg gaaaccttgc ccggcgcagt ggcgttttcg
    44401 atgctggaga atctggcagt agtgctgatt gcccccgcgc tgccctgccc ggtgcgtaat
    44461 caggtgtcgg cagagaagcc ggactggtct acagtgccgt ttatcatggc cgatcaggga
    44521 ccggtgcgtc gccgcattga actgtggttt cgacgcaata aaatcagcaa cccgatgatt
    44581 tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg tggcgctcgg ctgtggtgtg
    44641 gcgctgttgc cggaagtggt gctggaaaac agcccggaac cggtgcgtaa ccgcgtgatg
    44701 attttagagc gcagcgatga gaaaacgccg tttgaactgg gcgtttgtgc gcagaaaaag
    44761 cggctacatg agccgctgat tgaagcattc tggaagattt tgccgaacaa caaatgacgt
    44821 tgtcgcgcgg gtaagcctga taagcgaagc gctatcaggc atttttccct aacccgccaa
    44881 aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca gctcatttag
    44941 ccgggtttcg aaatccggtt catggtcgcc gagttcgaac gctgccagca cgcgcccata
    45001 gtcggtaccg tggctgcgat agtggaacaa agaaatattc cagtgcgtac ccagcgtgtt
    45061 gaggaagcgc agcagcgcgc ccggtgattc cgggaattcg aagctgtaga ggcgttcctg
    45121 caacggatgt gacggacgcc cgccgaccat atagcgcaca tgcaacttcg ccatttcgtc
    45181 gtcggagaga tccaccacgc tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg
    45241 ctcttcgagg ccgcggctca ggcgcacgcc gacaaagatg caggcgtttt tggcatcggc
    45301 aaaacggtag ttgaactcgg tgaccgatcg cccgccaagc agttggcaga atttgaggaa
    45361 gctgcctttt tcttccggaa tggtcaccgc caacaacgct tcacgctgtt cgcccagttc
    45421 gcagcgttcc gagacgtagc gcaggccgtg gaagttcacg ttggcaccgg aaagaatatg
    45481 cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc ccgccagcgc
    45541 cagcgcgcca gagggttccg ccaccgcacg cacatcttcg aataaatcct tcatcgccgc
    45601 acagatcgca tcgctatcga cggtgatgat gtcgtcgaga tactcctggc ataaacggaa
    45661 ggtttcgtca ccgatgcgtt ttaccgcgac accttcggcg aacagtccca cgcgtggcag
    45721 atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt ccgcttctac
    45781 ggcgatcact ttgatttgcg gcatcagttg tttgatcagc accgctacgc ccgccgccag
    45841 accgccgccg ccgactggca caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc
    45901 cagcgccagc gtgccttgcc cagcaatcac catcggatgg tcgaacggcg gcacccaggt
    45961 gaacccctgc tgctgtgaca gttcgatcgc tttagctttc gcttcgtcaa agttcgcgcc
    46021 atgaagcagc acttcgccgc cgaatccacg taccgcatcg actttgatgt cggcggtggc
    46081 ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg cgacgccctg
    46141 cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttctgttctt ccgtcaggcc
    46201 cgccatcatg gcgtatgcgc cgcgcagctt aaagctatga actggctgac gatcttcgcg
    46261 cttcaccaga atgacgttat caagacgaga cgagagcttt tccatttttt gtagcggcgt
    46321 gacctgcgcc gcttcgtaga ccggcgcgcg cagcaccgct cttaaatatt cggcaccttc
    46381 cggagcaccg gacaggggtt gtgagtcagc catcattaac cccccagttt cgatttatcg
    46441 cgcaccgcac ctttgtcggc gctggtcgcc aggctggcgt aggcacgcag ggcaaaggaa
    46501 acctgacgtt cacggttttt cggcgtccag gctttgtcac cccgagcttc ctgcgcttca
    46561 cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt cgggatgtcg
    46621 atagcgatca ggtcaccatc ttcaatcagg ccaatgctgc cgccgcttgc cgcttccggt
    46681 gagacgtggc cgatggaaag accagaggtg ccgccagaga aacgaccgtc ggtgatcagc
    46741 gcacaggctt taccgagacc cattgatttc aggaagctgg ttgggtagag catttcctgc
    46801 atgccagggc cgcctttcgg gccttcatag cggatcacca ccacatcgcc cgcgacaact
    46861 ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt cgtacacttt cgccgggccg
    46921 gtgaatttga ggatgctgtc atcgacgccc gcggttttaa cgatgcagcc gttttctgcg
    46981 aaattaccgt agagcaccgc caggccgccg tctttgctgt aggcgtgttc cagcgagcgg
    47041 atacagccat tggcgcgatc gtcatcgaga gaatcccaac ggcaatcctg cgagaatgcc
    47101 tgtgtggtac gaatgcctgc aggacctgcg cggaacatat tttttaccgc gtcatcctgg
    47161 gtcagcataa cgtcgtattg ttccagcgtt tgcggcaacg tcaggccaag tacgtttttc
    47221 acatcacggt tcagtaaccc cgcgcgatcc agttcgccga gaataccgat aacaccacca
    47281 gcacggtgaa catcttccat atggtatttc tgggtgctcg gcgcaacttt acacagctgt
    47341 ggaaccttgc gggaaagctt atcgatatca ctcatggtga agtcgatttc cgcttcctgc
    47401 gccgccgcca gcaggtgaag tacggtgtta gtcgatccac ccatcgcgat atccagcgtc
    47461 atggcgtttt caaacgccgc cttactggcg atattacgcg gcagtgcact ttcgtcgttt
    47521 tgctcgtaat aacgtttggt caattcaaca atgcgtttac cagcattaag gaacagctgc
    47581 ttacggtcgg cgtgggttgc cagcagcgag ccgttgcccg gctgcgacag gcccagcgct
    47641 tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggaacc gcaggtcgga
    47701 cacgcggaac gttcaacctg atcgctctgg gagtcagata ctttcgggtc tgcgccctgg
    47761 atcatcgcat caaccagatc gagcttgatg atctgatcgg aaagtttggt tttcccggcc
    47821 tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcaggga agccatcagc
    47881 atccccgggg tgattttgtc gcagttagag atgcagacca tggcgtcggc gcagtgggcg
    47941 ttgaccatat actcaacgga atcagcgatc agttcgcgag atggcagtga ataaagcatc
    48001 cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc tttggcaacg
    48061 ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag atggacgtga
    48121 cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttacc gaaatcggcg
    48181 tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg accatgagtg
    48241 gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt ctcgtaaatg
    48301 ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta accagcccca
    48361 tttatcttcg gtttcgccag tgaagaggcc gaagaaggct tgctgaatgc gtttggtaac
    48421 cgggccacaa cggccttcgc caacctgaat accgtctacg ctgcgcactg gcgtgatttc
    48481 tgccgccgta ccggacataa acacttcatc cgccaggtac agggattcgc gcgacagcac
    48541 ctgctcacgt acttcaattc ccagctcttt cgccagtttg atgatggcat cacgggtaat
    48601 acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt tcacttcaaa
    48661 caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga taccttcctg
    48721 ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagaggtagt taccaccggc
    48781 ttttgccgcc gtcgggatgg tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc
    48841 gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag cagcgataat
    48901 cacgtcggtt gagtatcccg ctggcgggtt tactcccatg ccaacatcac cgacgaagat
    48961 cagcggacgg atataggcgc tggtgagatt atttttgcgg atcacgtcac gacaagcttc
    49021 catcagctca tcaatgctct gcgaaaccgg gaagcgatag attttggcgg agtcatgcag
    49081 acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgcgagt cgtagcaacg
    49141 gatgccttca aaaaccgaag tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc
    49201 gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat
    49261 ttttatattc cttttgcgct caggcgcgga tttgttgtga tgtggttgtg ctctggcaga
    49321 tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag tcgaccgacc
    49381 gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg gcggccatat
    49441 tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt tctaaggttt
    49501 ccggattgaa gcgagccgat acattgacct gatgttgcat catgataatt tctccaacat
    49561 ttctgaatta ctggcgccag gcggcaccag cggccagacg ttctcaagtt cgtcgattga
    49621 gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg cttcaacctg
    49681 gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca tgaggaaatc
    49741 ggggttatca gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt gctgccattg
    49801 tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta actgcttgcg
    49861 ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg agatacagac
    49921 aacggtatcg ttcggtcgcg cgacttgtgc gccaaccgcc gccggtaaac caaaacccat
    49981 ggtacctaaa ccgctggagg tgatgaaatt ttccgggcga gtgtgggcga tgtgctgcgc
    50041 agcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag gtttacgatc
    50101 cgacagttgt tttaacaaca acggcgcgta gatagcgtca ccgggatggt cgtaacgcca
    50161 ggaatgttca tcacgcagct gcgcgcagtg ttgctgccag tcattgatat ttaacggctg
    50221 ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg cctgacgcag
    50281 cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg gcgcgaaggt
    50341 gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca gcaagtcgca
    50401 ctcctgcacc gcgaagttcg ccgctttggt gccgtgcatt cccagcatgc ccagatagta
    50461 cggataatct gcttcaactg cgcccagccc tttcagcgtg caggtggcag gcatttttgt
    50521 ggtagcgaga aattctcgta aagcaggaac tgcctgcgcc atacccacgc caccaccaac
    50581 gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcaa cttcggcatg
    50641 ggggaaagtc acttcgtttt caacggtggt gaaccacggt tccagatcgc cgctggctaa
    50701 ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc tggcaacgtc
    50761 gaatgctgca gccatgatgc gcggcaactc ttccagcgac tgcaccagga agctgtgttt
    50821 ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtcag tgccgataaa
    50881 cggtgcggac acttgaccgg tgatggcaac aacagggatg gaatctaaca gtgcgtccgc
    50941 aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata cgccagtttt
    51001 gccggtagca cgggcataac cgatagccgc cattgccgca ccctgctcat gtcggcatag
    51061 caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg cgccacccgg
    51121 ataaccgaaa acggtgctca caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc
    51181 attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct gttattcatt
    51241 gtctgctcct cggttatgtt tttaaggtca aaaaaaaccc ccggaccttt cggtgcgggg
    51301 gtcttagttc gttaaggctt gatctctaag cctttcctcg tccaagtgca gccccgcacg
    51361 gtgggataat aatcaccacc acgctaatca cgaccaggct aatcactcgt agaagggctg
    51421 tcattttgtc ttttcttgca tcttgttcga aggaatgcct aaagagttac catagatttt
    51481 gcgaatagca caagatattt ttgggcctat ttttatgatg gctttaataa tttcaataga
    51541 aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaaa ttcaatttca gtataaatgt
    51601 tgttcccttc ctcgtagttc atcctttgtc cgctgcattt ttgcgagcat cattccaccg
    51661 ccagaacaac tcaacttcac ttgcacaaat aaacaggaat cagccactaa aattcacgaa
    51721 ttagcgggct acagcagcga tatcttatgc acataatggt gactcaagga gggcttatgt
    51781 cactgtcaat tgttcatacc cgcgcagccc tgggagtaaa tgcgcccccg atcactgttg
    51841 aggtacatat cagtaaaggt ctacccggct taacgatggt gggcttacca gaaacaactg
    51901 taaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa tagcggatat gaatatccgg
    51961 cgaaaaaaat caccatcaac cttgctccag ccgatctgcc aaaagaaggg ggacgatatg
    52021 atttacctat cgccattgcg ttgctggcgg cctcagaaca gcttacagcc aataagttag
    52081 atgaatatga attagtcgga gaactggctc ttacaggcgc tctgcgtggc gttcccggtg
    52141 caatctccag tgcaactgaa gctattaagt cgggcagaaa aattatcgtc gcgaaagata
    52201 acgaagatga agtggggcta attaacggtg aaggatgcct gatagccgat catctgcaag
    52261 ctgtctgtgc gtttctggaa ggtaagcacg ctctcgaacg cccgaaacca actgatgcag
    52321 tatcccgggc gctacaacat gatctcagtg atgttgtcgg tcaggaacaa ggaaagcgag
    52381 gactggaaat taccgccgct ggcgggcaca accttttact gattgggccg ccgggaacag
    52441 gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc agatttaagc aatgaagagg
    52501 cactggagag tgctgcgata ttaagtctgg taaatgctga atcagtacaa aaacaatggc
    52561 ggcagcgccc gttccgctca cctcatcaca gtgcatcgtt aactgcgatg gtaggcggtg
    52621 gcgcaattcc agggcccggt gaaatttcgc tggcgcataa cggcgtgctt tttcttgatg
    52681 agttacctga atttgaaagg cgtacactgg atgccttgcg agagccgatt gaatccgggc
    52741 agatccatct ttcacgcacg cgagcaaaaa taacctatcc agcccgtttc cagcttgtcg
    52801 cggcgatgaa tcccagccct accggacatt atcagggaaa ccataaccgc tgcacaccag
    52861 agcaaacgct gcattatctc aaccgactct ctggtccctt tctcgaccgc ttcgatctct
    52921 cactggagat cccattaccg cctcccggta ttttgagtaa aacggtagtg ccgggagaaa
    52981 gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt aagcggcaga
    53041 ataaactgaa cgcctggctg gatagtccgg aaatacgcca attctgcaaa cttgagagcg
    53101 aagatgcgat gtggctggaa aaaacgctga tccatctggg gttatcgatt cgtgcctggc
    53161 agcggttatt gaaagttgca cgaaccattg ctgatattga tcagtctgac attatcacac
    53221 gtcaacattt gcaggaggca gttagctatc gagcgattga ccgtttgctc atccatctgc
    53281 agaaactact gacataaaaa aagggcattt cgcccttttt attaatcgtc agaatcggtg
    53341 tagtcttcag caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga
    53401 cgcttaatac gcgtcatata cttggaccac acgcgttctg cttctgtcac tggctcacgt
    53461 tcgccacggc atactgctac gaagagtttc tcttcctcgg taaccggctc gcgtttgcca
    53521 agatccaact cattgaaggc ataaccatga cgctcaagca gttgtgcctc tttgatggtg
    53581 aaatcaccat gacgagagaa tccacgtgga taatgtttat tgtcgaaata tcgattagtc
    53641 gtcgtaaagc tttccgccat cctgcacgct cctaattctt tgaccgagct agttatggcg
    53701 cggagtatta gttacgcttg acagagtgta aaacaaaaca tttaaatcat aacgacaaat
    53761 aattttgcgg agagcactgt ggatacggaa ttgttaaaaa ctttcctgga agttagccga
    53821 acgcgtcact ttggtcgagc ggctgaatcg ctctatctga cccagtcagc agtgagcttt
    53881 cgaatcagac aactggaaaa tcaactgggt gtgaaccttt tcacccgcca cagaaacaat
    53941 atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct catgagcacc
    54001 tggcaggccg cccgcaagga ggtggcgcat acctcacgac ataacgagtt ttctatcggt
    54061 gccagcgcct cattgtggga atgtatgctt aatcagtggc tgggacgttt gtatcaaaat
    54121 caggatgccc atacaggctt acagttcgaa gcgcgaattg cccaacggca gtctctggta
    54181 aaacagctgc atgaacgcca gcttgatctt cttattacca ctgaagcgcc caaaatggac
    54241 gaatttagta gtcagttgct gggatatttc actttagcgc tttataccag tgccccttca
    54301 aaactaaagg gagatcttaa ttatctgcga cttgagtggg ggccagattt tcaacagcat
    54361 gaggcaggtt tgatcggtgc tgacgaagtg cccattctga caaccagttc tgctgaactg
    54421 gcacagcaac agattgcgat gcttaatggt tgcacctggc tacccgtcag ctgggcgcgt
    54481 aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac tttcacggcc gctttatgcc
    54541 atatggctgc aaaatagcga taaaaatgcg ttgattcgcg atcttttgaa aattaacgtg
    54601 ctggatgaag tgtattaata tgaatggctg gcaaggatgc cggtagaagg atttacttcg
    54661 gagagggtta tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggcagg
    54721 ggcggagaga ctcgaactcc caacacccgg ttttggagac cggtgctcta ccaattgaac
    54781 tacgccccta attag
//
LOCUS       DAJSKJ010000005        54658 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000005 DAJSKJ010000000
VERSION     DAJSKJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.005"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..390
                     /locus_tag="ON345_000257"
     CDS             <1..390
                     /locus_tag="ON345_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR3070373.1"
                     /translation="GFNGNVISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGERI
                     DYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDK
                     FSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(478..1776)
                     /gene="exuT"
                     /locus_tag="ON345_000258"
     CDS             complement(478..1776)
                     /gene="exuT"
                     /locus_tag="ON345_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR3070374.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2259..3671
                     /gene="uxaC"
                     /locus_tag="ON345_000259"
     CDS             2259..3671
                     /gene="uxaC"
                     /locus_tag="ON345_000259"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3070375.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3686..5173
                     /gene="uxaA"
                     /locus_tag="ON345_000260"
     CDS             3686..5173
                     /gene="uxaA"
                     /locus_tag="ON345_000260"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR3070376.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELNFGLECGGSDGFSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IYGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5256..5794
                     /locus_tag="ON345_000261"
                     /pseudo
     CDS             5256..5794
                     /locus_tag="ON345_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
     gene            complement(5799..7043)
                     /gene="sstT"
                     /locus_tag="ON345_000262"
     CDS             complement(5799..7043)
                     /gene="sstT"
                     /locus_tag="ON345_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR3070377.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7442..8407)
                     /gene="alx"
                     /locus_tag="ON345_000263"
     CDS             complement(7442..8407)
                     /gene="alx"
                     /locus_tag="ON345_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR3070378.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWMNYRHDKQRGG"
     gene            complement(8690..9676)
                     /locus_tag="ON345_000264"
     CDS             complement(8690..9676)
                     /locus_tag="ON345_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3070379.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSLYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(9755..10447)
                     /locus_tag="ON345_000265"
     CDS             complement(9755..10447)
                     /locus_tag="ON345_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR3070380.1"
                     /translation="MLRAFARFLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(10524..11027)
                     /locus_tag="ON345_000266"
     CDS             complement(10524..11027)
                     /locus_tag="ON345_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR3070381.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11112..12248
                     /gene="rlmG"
                     /locus_tag="ON345_000267"
     CDS             11112..12248
                     /gene="rlmG"
                     /locus_tag="ON345_000267"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR3070382.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            12533..12847
                     /gene="higB"
                     /locus_tag="ON345_000268"
     CDS             12533..12847
                     /gene="higB"
                     /locus_tag="ON345_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR3070383.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            12844..13260
                     /gene="higA"
                     /locus_tag="ON345_000269"
     CDS             12844..13260
                     /gene="higA"
                     /locus_tag="ON345_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR3070384.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13305..15323)
                     /gene="fadH"
                     /locus_tag="ON345_000270"
     CDS             complement(13305..15323)
                     /gene="fadH"
                     /locus_tag="ON345_000270"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR3070385.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(15649..18000)
                     /gene="ygjK"
                     /locus_tag="ON345_000271"
     CDS             complement(15649..18000)
                     /gene="ygjK"
                     /locus_tag="ON345_000271"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3070386.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(18017..19087)
                     /gene="ygjJ"
                     /locus_tag="ON345_000272"
     CDS             complement(18017..19087)
                     /gene="ygjJ"
                     /locus_tag="ON345_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCR3070387.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEVRPAEHDDTKTPAITSMS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(19221..20654)
                     /locus_tag="ON345_000273"
     CDS             complement(19221..20654)
                     /locus_tag="ON345_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3070388.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASVPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(20717..21166)
                     /locus_tag="ON345_000274"
     CDS             complement(20717..21166)
                     /locus_tag="ON345_000274"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR3070389.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21163..24255)
                     /gene="ebgA"
                     /locus_tag="ON345_000275"
     CDS             complement(21163..24255)
                     /gene="ebgA"
                     /locus_tag="ON345_000275"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR3070390.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGRRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNSNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(24439..25422)
                     /gene="ebgR"
                     /locus_tag="ON345_000276"
     CDS             complement(24439..25422)
                     /gene="ebgR"
                     /locus_tag="ON345_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR3070391.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDELGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            25641..25973
                     /locus_tag="ON345_000277"
     CDS             25641..25973
                     /locus_tag="ON345_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR3070392.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(26015..27394)
                     /gene="ygjG"
                     /locus_tag="ON345_000278"
     CDS             complement(26015..27394)
                     /gene="ygjG"
                     /locus_tag="ON345_000278"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR3070393.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            27812..29332
                     /gene="aer"
                     /locus_tag="ON345_000279"
     CDS             27812..29332
                     /gene="aer"
                     /locus_tag="ON345_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR3070394.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSEMLAK
                     GTDELNKHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAERAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29486..30109)
                     /gene="nfeR"
                     /locus_tag="ON345_000280"
                     /gene_synonym="yqjI"
     CDS             complement(29486..30109)
                     /gene="nfeR"
                     /locus_tag="ON345_000280"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR3070395.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30386..31150
                     /gene="nfeF"
                     /locus_tag="ON345_000281"
     CDS             30386..31150
                     /gene="nfeF"
                     /locus_tag="ON345_000281"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR3070396.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(31204..31279)
                     /locus_tag="ON345_000282"
     tRNA            complement(31204..31279)
                     /locus_tag="ON345_000282"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31244..31246),aa:Ile,seq:cat)
     gene            31404..31910
                     /gene="mug"
                     /locus_tag="ON345_000283"
     CDS             31404..31910
                     /gene="mug"
                     /locus_tag="ON345_000283"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR3070397.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(31989..33830)
                     /gene="rpoD"
                     /locus_tag="ON345_000284"
     CDS             complement(31989..33830)
                     /gene="rpoD"
                     /locus_tag="ON345_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR3070398.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(34025..35770)
                     /gene="dnaG"
                     /locus_tag="ON345_000285"
     CDS             complement(34025..35770)
                     /gene="dnaG"
                     /locus_tag="ON345_000285"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3070399.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDVGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMDQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(35881..36096)
                     /gene="rpsU"
                     /locus_tag="ON345_000286"
     CDS             complement(35881..36096)
                     /gene="rpsU"
                     /locus_tag="ON345_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR3070400.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            36334..37347
                     /gene="tsaD"
                     /locus_tag="ON345_000287"
     CDS             36334..37347
                     /gene="tsaD"
                     /locus_tag="ON345_000287"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR3070401.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(37390..38853)
                     /gene="ttdT"
                     /locus_tag="ON345_000288"
     CDS             complement(37390..38853)
                     /gene="ttdT"
                     /locus_tag="ON345_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR3070402.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(38902..39507)
                     /gene="ttdB"
                     /locus_tag="ON345_000289"
     CDS             complement(38902..39507)
                     /gene="ttdB"
                     /locus_tag="ON345_000289"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR3070403.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(39504..40414)
                     /gene="ttdA"
                     /locus_tag="ON345_000290"
                     /pseudo
     CDS             complement(39504..40414)
                     /gene="ttdA"
                     /locus_tag="ON345_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            40621..41553
                     /gene="ttdR"
                     /locus_tag="ON345_000291"
                     /gene_synonym="dan"
     CDS             40621..41553
                     /gene="ttdR"
                     /locus_tag="ON345_000291"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR3070404.1"
                     /translation="MLNSWRLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMHNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(41566..42183)
                     /gene="plsY"
                     /locus_tag="ON345_000292"
     CDS             complement(41566..42183)
                     /gene="plsY"
                     /locus_tag="ON345_000292"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR3070405.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            42288..42656
                     /gene="folB"
                     /locus_tag="ON345_000293"
     CDS             42288..42656
                     /gene="folB"
                     /locus_tag="ON345_000293"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR3070406.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            42746..43567
                     /gene="bacA"
                     /locus_tag="ON345_000294"
     CDS             42746..43567
                     /gene="bacA"
                     /locus_tag="ON345_000294"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3070407.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(43730..44968)
                     /gene="cca"
                     /locus_tag="ON345_000295"
     CDS             complement(43730..44968)
                     /gene="cca"
                     /locus_tag="ON345_000295"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR3070408.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDSLFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(45032..45652)
                     /locus_tag="ON345_000296"
     CDS             complement(45032..45652)
                     /locus_tag="ON345_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR3070409.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            45894..47195
                     /gene="ygiF"
                     /locus_tag="ON345_000297"
     CDS             45894..47195
                     /gene="ygiF"
                     /locus_tag="ON345_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR3070410.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            47218..50058
                     /gene="glnE"
                     /locus_tag="ON345_000298"
     CDS             47218..50058
                     /gene="glnE"
                     /locus_tag="ON345_000298"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR3070411.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            50106..51539
                     /gene="hldE"
                     /locus_tag="ON345_000299"
     CDS             50106..51539
                     /gene="hldE"
                     /locus_tag="ON345_000299"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR3070412.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(51659..51718)
                     /gene="ibsE"
                     /locus_tag="ON345_000300"
     CDS             complement(51659..51718)
                     /gene="ibsE"
                     /locus_tag="ON345_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR3070413.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(52035..52094)
                     /locus_tag="ON345_000301"
     CDS             complement(52035..52094)
                     /locus_tag="ON345_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR3070414.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(52333..53994)
                     /locus_tag="ON345_000302"
     CDS             complement(52333..53994)
                     /locus_tag="ON345_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR3070415.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(54021..54650)
                     /locus_tag="ON345_000303"
     CDS             complement(54021..54650)
                     /locus_tag="ON345_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR3070416.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
BASE COUNT        12920 a        14126 c        14674 g        12938 t
ORIGIN      
        1 ggttttaacg gtaatgtcat cagttatgaa atcatgctca aagatgaagg gcgaccagct
       61 gcagggcgca gagacggtta cttctctatc tatcgtcagg gagggaccac caccgacgag
      121 ggagaacgca ttgattaccg ggtcaaaatg tacaacccgg aaaccggtgg gcaaattgat
      181 gtgcgcaata atgaaaatat ggtctggaac agcattaacc tgaaacgtgt gcgtccggtg
      241 gtactgcccg gtattcgcta tgccgtgatg tgtgtgccga cacccttgac tctggcagtc
      301 gataaattca gcgtgatgga taaacaggcc gggtactaca tgggcaaatt gtcagtaatc
      361 tttacgcctt ccttgccaac catcaattaa ccctgcaaac cggacaaacg cttttttcta
      421 tataaacgac gaagccgtct gataaggcgg cttcgtcatt acaacttaag ttaaacgtta
      481 atgttgcggt gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg
      541 caacacggtc cagataacca gcgcacccag caggtcaaat actgccagaa ctgcgaacag
      601 cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc
      661 cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac
      721 gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca
      781 cagcagcata attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat
      841 cagcacagca cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca
      901 acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat
      961 cggcatccag gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa
     1021 cagcgggatc cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagtgc
     1081 gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct
     1141 cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg
     1201 cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa
     1261 tgcaccggag atgataaatg ccatctgcca gctgtgcatc acgattgccc ataccaccag
     1321 cggcggcgca atcatcgcac caatcgaaga acctacgtta aagtagccta ctgcgatgga
     1381 acgctctttc gccgggaacc attcggagct ggctttcaga cccgccggga tcatcgctgc
     1441 ttccgcggca ccgaccgcac cacgagcaac agccaggcca ccccagctac ctgccagcgc
     1501 ggttgcacca cagaacacgg cccacagcac agcaaacatt gcataaccga ttttcgtacc
     1561 cagcacatcc agtacataac ctgctaccgg ttgcatgacc gtataagcag cagaataggc
     1621 tgcgatgata taggaatact gttgggtgga gatgtttaac tcttccatca gagttggcgc
     1681 agctgccgcc acagtgttac gcgtcaggta accaagcacg gtgccaagcg tcaccagtgc
     1741 gatcatatac caacgtaacc ctttaatttt acgcatgtaa aacctcatcg ttttgttata
     1801 tccgcaggaa gcgcggccat catcccgcca gaggctgaag atgttagtta cgaccgggta
     1861 accgaaaaaa aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt
     1921 ccgtaccggt taacccgatt catctaacaa atgaatgaat cggcaatttg cattccgtcg
     1981 acttatgatt tgcgacggca gaaagataac ttgtcataca actttaaaag gtgagagcca
     2041 tcacaaatgt gcgaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg
     2101 cggcctgcag cgagatttac cactttgaga gtaatttttt taactacgtt tattgatcta
     2161 actcacgaaa atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc
     2221 ataagcaagc tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag
     2281 atttcctgtt agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc
     2341 cgattttcga ttaccattgc catttgccgc cgcagcagat tgcggaagac tatcgtttta
     2401 aaaacctgta tgacatctgg ctgaaaggcg atcactacaa atggcgcgct atgcgtacca
     2461 acggtgtggc cgagcgtctg tgtaccggtg atgcgtctga ccgtgaaaaa tttgacgcct
     2521 gggcggcgac tgttccgcac actatcggca acccgttata ccactggacg cacctcgaac
     2581 tgcgtcgtcc gtttggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct
     2641 ggaacgaatg taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc
     2701 agatgaacgt gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg
     2761 cagagatcgc caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca
     2821 aagccttcaa catcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt
     2881 ccgataccga cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc
     2941 acttcgccgc tcacggctgt aaagtgtctg accatgcgct ggacgtggtg atgtttgctg
     3001 aagcgaacga agcggaactg gacagcattc tggcgcgccg tctggctggc gaacccctga
     3061 gcgagcacga agtggcacag ttcaaaactg cggtactggt gttccttggt gccgaatatg
     3121 cacgtcgcgg ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc
     3181 agttcaaact gctggggccg gatgtaggct ttgactccat caacgaccgt ccgatggcgg
     3241 aagagttgtc taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc
     3301 tctactgcct gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg
     3361 gcgaaggtat gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag
     3421 acggtatgga acgtcagatg acccaactgg cgcagctcgg tctgctgagc cgctttgtcg
     3481 gtatgctgac tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca
     3541 ttctgtgcca gatgatcggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc
     3601 tgctgggcga gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg
     3661 aactgaacta aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt
     3721 cgcggtcgct ttagcagatt tggctgaagg cacagaagtc agtgtcgata accagactgt
     3781 tacgctgcgc caggatgttg ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg
     3841 ggccaacgtc atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg
     3901 ggaacacgtc cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta
     3961 tcaacctgat tttcaggatc tgcctgcgca agcggcagat cgtgaagtgc agatctatcg
     4021 tcgcgctaac ggcgatgtcg gggtgcgtaa tgagctgtgg atcctgccaa ccgtgggctg
     4081 tgtcaacggc atcgcgcggc aaatccagaa ccgtttcctg aaagagacca acaacgccga
     4141 aggtaccgac ggcgtgttcc tcttcagcca cacctacggc tgttcacaac tgggcgacga
     4201 tcacatcaac acccgcacca tgctgcaaaa catggtgcgc cacccgaacg cgggcgcagt
     4261 gctggtgatt ggtctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgttggg
     4321 cgatatcgat cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga
     4381 agccgggatc gagcatttgc atcagctgta taacgtgatg cgcaacgata aacgcgagcc
     4441 aggcaaactc agcgaactga attttggtct ggagtgcggt ggttctgacg gtttttctgg
     4501 tattactgct aacccgatgc tggggcgttt ctctgactac gtgattgcta acggcggtac
     4561 taccgtactg accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg
     4621 ccgcgacgaa gcaacgtttg aaaaactggt caccatggtc aacgacttca aacagtactt
     4681 tattgcccat gatcagccga tctacgaaaa cccatcaccg gggaacaaag cgggcggtat
     4741 caccacgctg gaagacaaat cacttggctg tacccagaaa gcgggttcca gtgtcgtggt
     4801 tgacgtgctg cgttacggcg agcgtctgaa aacgccaggg ctgaacttgt taagtgcgcc
     4861 gggtaacgat gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt
     4921 cagtactggt cgcggcacgc catacggtgg atttgtgccg acggtgaaaa tcgccaccaa
     4981 cagtgaactg gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcta
     5041 cggtaaagcg atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa
     5101 cggtaagcaa acctgcaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg
     5161 cgtaacgcta taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc
     5221 gccgtttgct tttccatacc tgctaaggat ttatcatgac cgcgtattgg ctggcccagg
     5281 gcgtgggtgt catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc
     5341 gcttcaaaaa gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg
     5401 gcacctatcc cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac
     5461 gcacgcgcag cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg
     5521 cgaagttcca tcatcctgtc gaactattgc cggttatcgg tacgattgtc agtacctggg
     5581 cgctgttccg ctgtaaaggg ctcaccatgc gctgcgtgat gtggttttca acgtgttgct
     5641 gggtgattca caacttctgg gcggggtcga tagggagggt agttttctgc ttatgaatgg
     5701 cctgaatatc attcgtttct ggcggatgca gaaaagggga attgatccgt ttaaagtaga
     5761 gaaaacccct cccgccgtag acgaaagggg ttaaacaatt aattacgcag ggcgctattt
     5821 gccagacgat cgtcttccgc ctggcaagct gccgcagtga acagcacgtc agttgaagag
     5881 ttcagcgcgg tttcgcaaga gtcctgcaat acgccgatga taaagccgac ggcaaccacc
     5941 tgcatggcga tatcgttcga aataccgaac atattacagg ccagcgggat cagcagcaga
     6001 gaaccccccg ccacgccgga tgcgccacag gcacacagag aagccaccac gctcaacagc
     6061 agcgccgtgg gcagatcgac cgggataccc agcgtattaa ccgcagccag cgtcaacacg
     6121 gtaatggtga ttgctgcgcc cgccatattg atggtggcac ccagcggaat agaaacggaa
     6181 taggtatcgc gatccagatt cagcttttca cacagtgcca tattcaccgg aatgttcgcc
     6241 gcagagctgc gggtgaagaa ggcatacacg ccgctttcgc gcaggcacag cagcaccagc
     6301 gggaacgggt tacgacgaat tttccaccac accagcaatg ggttaaccac cagcgccacc
     6361 agtaacatac agccaaccag cacgaccagc agttgcgcgt agccccacag tgtggagaaa
     6421 ccggtggttg ccagggtaga agaaaccaga ccaaaaatac caatcggtgc gaagcgaatg
     6481 accagtttca ccataaaggt gacggcattc gacatatcgt tgaccaggtt tttggtggtc
     6541 tcgttaccgt gacgcagtgc gaaaccgagg ccaattgccc acaccaggat cccgatgtag
     6601 ttacctttca gcagcgcgtc gatggggttg gaaaccatgc tcattaccag cccgcgcatc
     6661 acttctacaa tgcctgacgg tggcgaaata tcacccgcgc tactggataa gtgcagggta
     6721 gaagggaagg cgaagctgaa aacgactgcg gccagagcag cagagaaggt acccagcaga
     6781 tagaggaaca aaatagggcg gatattggtt ttctgcccgt gctggtggtt agcaatagat
     6841 gccatcacca gcatcaacac caggatgggg gcaacggctt tcagtgcgcc gacgaacaaa
     6901 gtacctaaca gaccaacagc ttccgccgcg ggttttgaga tccatgccag aagaatcccc
     6961 agaacaaggc cgaccaggat ttgttttacc aggctgccat gagccagacg ccggaatagc
     7021 cccggtgaac gttgcgtagt catttttcga tcctttcatt gtgttgtcgc acatccctgg
     7081 tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt ttgcaaggtc gagtataagg
     7141 aaagttacag cttcgggaag cgaaaaatgc tggattttaa agagaggtca tattttgcaa
     7201 cttaatggtt acatttattt gacataaaaa taaagccaac gtgcggcttc cagactgatg
     7261 acaacatcaa aaaaggactc ggtcagtccc ctcgccccgt ttggggagag ggttagggtg
     7321 aggggaacag gccagcatcg gtgcgaaaaa ttaccctcac cccgaccctc tccctggaag
     7381 ggagagggag aaaacggtgc tcatgttaat gacagggtac acggcttagg cagattaaaa
     7441 attatccacc ccgctgctta tcatgccgat aattcatcca ggcgttgata ataaacgtca
     7501 tcaccagaat gccaaacacc acgcccagcg agacggcgat tggaatatgg tagaagtcga
     7561 caatcagcat cttgataccg ataaacacca ggatcaccgc caggccatat ttgagcatcg
     7621 agaaacgctc tgctacgccc gccagcagga aatacatcgc acgcaggccg aggatcgcaa
     7681 acaggtttga ggtcagcaca atgaacgggt cagtggtcac ggcgaagata gccggaatgc
     7741 tatccacggc gaaaatcacg tcgctcaatt ccaccagaat cagtaccagc atcagcggtg
     7801 tggcgtacag caatccattc ttacgcacaa agaaatgctc gttgtcgatg gtgtcggtca
     7861 tgcgcaaatg accgcgtagc cagcgcacca gcggcttgtc gccaatgcct gattcatctt
     7921 catgggcgag cgccatcttc acgccggtaa acagcaggaa ggcaccgaag atatacagga
     7981 tccagtcgaa ctgcgaaatt agccagctgc cagtgaagat catgatggta cgcagaacaa
     8041 tcgccccgag cacgccatac accagcacgc ggcgttgtaa tgccgccgga acagagaaat
     8101 agctgaacaa catcagccag acaaagacgt tatcgaccgc cagcgatttc tcaatcagat
     8161 aacctgtgag aaaggccagt gcctgtggat cggcaacggc gcgaccttcg gtttgcacca
     8221 gataccacca gaaagcggcg ttaaacagta acgacagcgt cacccagacc agcgaccagg
     8281 ccgcagcctg tttcatggtc atggcatgtg ccccacgacg cccctgcaac aacaggtcga
     8341 tagccagcat aatggcgaca acaacagcga atccgcccca tagcaacggc gtgccgacag
     8401 tattcataga agttccttac acataaaaaa acggccaacg tcggaagacg tcagccgctg
     8461 ctttttatgc atagacctcg ccttccggca aggtctcact tacaacaaaa aaggaatacg
     8521 tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg tttttcacac atcgtaatga
     8581 cgatcgaccg gcaatgaagt tactcccctt tgcaggtaac aaagtatgac agaccattca
     8641 gccagtcaat ggtctgttac atacctttta cactgtttta ctttggcaat tatagtttta
     8701 cgctatctgc cggaaaaatc accccagtct ggcggcggat ctcggtcagc agtttggcgg
     8761 tgatgcgact gaccgccagc cccggatgat ccaccagatg ctcatccacc agggtagcga
     8821 acagctctgc ttcgtagagc atggtattaa tatgctgcgg ctgggtgaga tcctgcattt
     8881 ggctgccacg cggcacgaag cacactttct ggcattcaga cagtttttca atcaccagcg
     8941 atcctgcttc gccctgaatc tcgctcgcca ggacagaatc actgactttt gagtgctgca
     9001 aggtgacgct gaaatcaccg taatccatca ccaccacgcc gtgggcatct acaccgcttg
     9061 ccagcaaact ggcggttgcc tgcacgcttt tcggctcacc aaataacgcc accgccgacg
     9121 ccaggcagta aaagccgata tccataattg aaccgttaga gaatgacgga ttaaaggtgt
     9181 tgggattttc accgtccagg taacgttgat agagcgagga atactggcaa tagttgaaaa
     9241 agactttacg caatttgccg actttcggca gcgcctggcg cagcaaatga aagttcggca
     9301 ggcaggcggt tttaaatgcc tcaaacagca ccacctgatt ttcccgcgca caggcaatgg
     9361 cggcatccac ttccgccaga ttcgacgcca gtggtttctc gcaaatcaca tgaattttat
     9421 ggctaaggaa aagttgtgtc tgggaaaagt gcagggcatt cgggctggca atatacaccg
     9481 cgtcaatggc atcgctttcc gccatcgctt ccagcgaggt aaacagatgc tcgacagaaa
     9541 aatcattggc gaagtgctga gcctgctcaa ggctgcggga atatacggcg gttaacttgt
     9601 atttaccgct ctcatgggcg gcctcgacga actggcgagt gatccagttc gtaccaatca
     9661 cagcgaaacg tatcataacg cgtggatact ccataaaggg attctgtcgt caatgtagca
     9721 cgcctttttc atcttcgacg tgcgcagata cgcattattc ttttgccgga cagccatgct
     9781 cactaaacgg cccaatcatg accgatggcc cgagatattt cggctggcga tggaggattt
     9841 tggcgtcaat cacggcgctg acacgagcga gtttctcccg cagttgagta taaaaaccgt
     9901 gcttcatatt gaggtcaggc gcgttaaaac cgatagcgtc gatgctgtac tgttttgcca
     9961 gccagatggc gcgttggtta tgaaattctt gcgagataat ggtgatatgg ttttcaccaa
    10021 aaaccttttt ggcacgcact accgaatcca gcgttgagaa tccggcatag tcgcagaaga
    10081 tcactttcgc cggtacgcct ttagcgatca aagccttctg catacctgag gcttcatcgt
    10141 aatttttacg tccgttatcg ccacttacca gtagccattt cacttttccg gcgtggtaca
    10201 acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt acccggcctg gcccccagca
    10261 ataagcccac gttacgcgcc ggaactgcgt tgacatcgct ccaggtcagc tgcttgctgg
    10321 cgttgaccat tactcgatcg gcgataaaaa tggtcgctcc tgccacaagt aacagtaagc
    10381 aggcaatctt tagagtccgg cgagaaaaac aaatacggag aagaaagcgg gcaaatgcgc
    10441 gcagcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaga
    10501 aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc
    10561 cacagccggg tgtcgaactc aagctggtgg tactgcggct ccatatggca gcacaattga
    10621 tagaacgctt tgttatgctc tttctccttc aggtgcgcca gctcgtgcac cacgatcatg
    10681 cgcaaaaacg gttccggcgc gttgcgaaac acggtagcga cgcggatctc cgcttttgct
    10741 ttcagcttgc cgccctgcac acgagaaaca gcggtatgta acccgagcgc gttttttagc
    10801 acatggatct tattgtcata catcacttta ttgatcggcg gggcattgcg cagaaactga
    10861 ttttttagat cctgagtata ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc
    10921 ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagcgt tcgcacctgg
    10981 gaaagtagct gctcgggata accctgaaga taagtaagat tgctcataac cgcccacgaa
    11041 aaagagaaaa agggtatact cacgcaccct tttcaggggc aacgtcgaaa ttttatcatt
    11101 caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc
    11161 cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa tatttgctgc
    11221 aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg
    11281 cgttaagctg cgcgctggcg gaacataagc cgtacagcat tggcgactca tacatcagtg
    11341 aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc
    11401 tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa gtgccgaaaa
    11461 cactggcatt gctggaacaa caactgcgtg cgctgcgcaa agtggtcacg tcggatacac
    11521 gtattattgc cggtgccaaa gcccgtgaca ttcacacttc cacgctggaa ctgttcgaaa
    11581 aagtgctcgg cccgaccacc accacgctgg catggaaaaa agcgcgcctg attaattgca
    11641 cgttcaatga accgccgctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta
    11701 ctgactggac tatccataac catgcgaatg tcttctcccg caccgggctt gatatcggcg
    11761 cgcgcttctt tatgcaacat ctgccagaga atctcgaagg ggagattgtc gatctcggtt
    11821 gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa cccgcaggcg aaagtggtgt
    11881 ttgtcgatga atcgccgatg gcggttgctt ccagccgttt gaacgttgaa accaacatgc
    11941 cagaggcgtt ggatcgcagc gagtttatga tcaacaacgc gctctccggc gtggagcctt
    12001 tccgctttaa tgctgtgctc tgcaacccgc cgtttcacca acaacatgcg ctgaccgata
    12061 acgtcgcctg ggagatgttc caccatgccc gccgctgcct gaaaatcaac ggcgagctgt
    12121 atattgttgc caaccgtcac ctggattact tccataaact gaagaagatt ttcggcaact
    12181 gcaccaccat cgccacgaat aataaatttg tggtgctgaa agcagtgaag ctggggcgtc
    12241 gtcggtaaga ttgggagtta cctgccggat gcggcgttaa cgccttatcc ggcctacgtt
    12301 cagcacgtta ctcccaggcc ggataagacg cggcaagcgt cgcatccggc aatgtctgcc
    12361 cagttctgga atcgcgcttc cataaaacaa aaaatccctc tcaccatcct ctacattctc
    12421 ttgtttagcg tttttctacg tttattcttc cgtcacacag ataaattcat ccgttgccaa
    12481 tctgtcaacg gatgttatca tattgccaat ttgtcaacgg acgtgatgaa gaatgcacct
    12541 gataactcaa aaagcattga aagatgctgc ggaaaaatac ccgcaacata aaacggagtt
    12601 ggtggctctg gggaacacga ttgctaaggg atatttcaaa aaacctgagt cattaaaagc
    12661 agtattccca tctctggata acttcaaata tctggataag cattatgttt tcaatgttgg
    12721 gggcaatgaa ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt gctacatacg
    12781 tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta ctaaggggaa
    12841 aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc tgtggcacct
    12901 tttctggcgg gtattcagaa cgaggaacaa tacacccagg cgctggaact ggtagatcat
    12961 ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgcaaa aataaccgcg
    13021 tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc
    13081 ggtatagccg tgattcgtac ccttatggat caatatggtt taaccctttc cgatctgccg
    13141 gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg
    13201 gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgattaa
    13261 cgcgacgtgc cggatgcgtt gcatccggcg cttcaaataa cgatttaaat ctccagcgcc
    13321 agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac atcgcagccg
    13381 ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc gcggtttggc
    13441 tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc gccgttgatc
    13501 accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat cattttcaca
    13561 ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa cccctgtccc
    13621 ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg gatctgcatt
    13681 ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca ctcattacag
    13741 aacctggcga tattctggct ggtggattcg cccggctgac ttaaatacat cgccgtatca
    13801 aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc tttgtcgcgc
    13861 agtacatcga gataactcaa taccttcgga tgatcgatcc cgtcgatagg cggaacgcgc
    13921 ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc cgccgtcacg
    13981 gtgtgattga gtttcagcgt cacgcccgtc acttcgatca tccggtggta atagcgcagc
    14041 gtttcgtaaa actcctcttt gccggggatc tgtttggcga tattaaactg cccgccgatc
    14101 tcgctgtggg catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt aatggcaaac
    14161 gccagcccag caggtcccgc accgaccacc gccagatttt ttttctgcac ggcaggaagg
    14221 attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt gactttgcca
    14281 acgaagatct gatcgaggca ggcctgattg cagccaatgc aggtgttgat ctcatcggct
    14341 cgccccgatt gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg tgccatcgac
    14401 accatatcgg catcgccgcg cgagagaatg tcgtcggcaa cctgcggatc gttaatccgg
    14461 ttggtggtca ccagcggcag cgagacgtgg cctttcagtt tgcgcgtgac ccaagtaaat
    14521 gcgccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca gccaatgccg
    14581 gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg ccagttctac cgtttcggca
    14641 aaagtgccgc cgccttcgac caggtcgagc atcgacaacc ggtagataat aatgaagtcg
    14701 ttgccgacgc gttcgcgcac cgtacgcact acttctacgg caaatcgcat ccggttgcgg
    14761 taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa ttcgttgatc
    14821 aaataccctt cggaccccat tacctctaca ccgtcgtatc ctgcctcccg tgccagttgc
    14881 gcgcagtggg cgaaatcgtc gatcagttgc aggatctctt catgggtgag ttcgtgagga
    14941 acgaaacggt tgatgggggc ctgcaatgcg gagggagcga ccagatgcgg ttggtagctg
    15001 tagcgcccgg tatgcaaaat ttgcagggct attttgccac cttcctgatg taccgcttcg
    15061 gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc accttccatg
    15121 ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac gccgtgacgg
    15181 gcgcgttcgg cataaaacgc tgccagccgc tcggcaccgt ccgggtattc ctccagcccg
    15241 gtgtgcattg agcccatcaa cacgcggttt tttaacgtgg taaaacctaa atccagcggg
    15301 gcgaacagcg acgggtagct cataaaattg tccagtatgt aaaataattg ttatgtggtc
    15361 ggatgagttc taatttagcc gtcgccggag aaaagggaaa aggggagtgc ggggtttgtg
    15421 atgggattca aaaagaatac gcccggtggt aacgccggat gcgacgcttg ccgcgtttta
    15481 tccggcctgg gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagtg
    15541 gcacggtgca gatgccggat gcgacgcttg tcgcgtttta tccggccagg aggattgcac
    15601 atgtagggcg gataaggcgt ttacgccgca tccgccagca gttgcaaatt attgcttacg
    15661 gaaaaaatcg ttatacaaca tatacaaatg cgcggcactc caggagaaat ttggtgcgcc
    15721 ttgctgtgcg ccagtcagcg ggttgtaatt ctcctgaatt gggccatcgg cggttaatcc
    15781 tttggcgtga cggaagaacg tatccgccag tttcagggca tcatcgcgat aaccgtaacg
    15841 ctccatccct ttcagaccaa accagaactg atccacccat acgcgcccac gccagtagat
    15901 atcagcgcca aaagccggat tggttaacgc cgccgttccc agcgggacaa aggtgttgaa
    15961 ctctttagga tcgagcatca ccttcaccac cgcgtcggca ttggcctgcg ttgccgcacc
    16021 gttaaacagc ggcgaccagc cttccggccc tttaccgcgc tcaacaatcg gtttgcccgc
    16081 gcagccgttc gccagtggtt tatcttcaat acgtacgtca tagtagaact gggtagtcgg
    16141 gtcgaacata caggtgttga tgtagtccgc gagctgctgt gccaactggc gatagcgttt
    16201 ggcctcttct ggcttaccga ggatggttgc catctccgcc agataatggt tatcgctgta
    16261 catatagctg gcctgatcca ccgactcctg caatagcgag tagcccagca acgttccgtc
    16321 ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta cgtttgccgc cattagcgac
    16381 atatttatcc aactgttctt tgtcgataaa accaaagacg gcggcatcat cacgccccga
    16441 ttcccacgac gcagcgacct gtgccggaat ttccagactg tcgtactggc ctttctccac
    16501 cacgcgggcg tagttgttca gcccagactg cgtctcttct ttgttgcctt tcttcaccgt
    16561 aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg cgggtcgcgc catattccgg
    16621 cacgccgttg ccgttgtgat cgcggttacg taaccaccag tcgtgatagg ccaccagttt
    16681 cgggtacatc tctgccaacc aggctttatc ctgggtgacg ttgtacactt ccatcaccga
    16741 ccaggcggca aggctgggtt tggtattacg ttcgttccag ttgccgccat cgccaccacg
    16801 ctcggggcta aggttccacg ctatcaggtc ggggacaaag cccacatcct gcggacgcac
    16861 gctatcgcca ggctggatct gccaggagaa gaccgcgcgg atattctctt tggcgatgtc
    16921 cggattgaaa tgcgccatcg caaacgcctg cttccaggta tcccacggcc aggtctgatt
    16981 gccggagaac cagcgcccgg tcaccgacgg tgtaacggtg ttaaatttca ccgcaccgcc
    17041 cggtgagcgc cagttaccgt tgagcgtttc gatcgctttc accgcgacgc gcgtctgttc
    17101 cggcgtcgca tccggattgg ttaacccttt cttcaggtat tcttcccagc gttgctgcga
    17161 ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg atctgcattt gctctttgct
    17221 aacttcctga gcggtcagca gatgggaata ggtggtgtag agcgtggtcg aaccgttgat
    17281 atgcgcctta ctggtaaagc gattaccgtt gatttcagtc tgcaccggca gggatttatg
    17341 cacctgatat tccgattcgc cggaggtcag cagatcccag gtggcgcgca ctttgccaaa
    17401 ggtgactttc aggccatcac gggtggcgct gattttgcgc tgatagtcag ggtattcgcc
    17461 agcaatggtt ttatcggaaa gcggtttccc ttctttcgct tccagttttt ccagcagttc
    17521 gccgtcccac accagatcca gcggtttatt gctggtgatt ttggtttcca gtagtgacgt
    17581 gcgcggcgtg gcgaagcgca gagtcatttc gacttgcaca tcttttgctg tcagtttttg
    17641 caccagcgca ccgggaatac tgtatgcctc cagcgtaaag tcgactttct tgccgtcctg
    17701 ccagacggtc aggcggtcga aattgctggc cataaagttg atgtactctt ccgtcagcag
    17761 cgcaacgccc ggaaagccgc ccatggtgtt agggccgtct ggcaacagat gaccatgcca
    17821 ggctccgaga tcgaaaaacg gattaaaacg ctggtgatcg tcgtaatcgt aatccttcat
    17881 gtactgcggt gcgccagtac ggttaatcac gtttttataa ttgtcggcgt tagcggcatg
    17941 ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt aaaatagttt ttattttcat
    18001 gaaattacag tccttattac cagtagaaat attgcatcag gaagatttgg tcgctgggat
    18061 cgtgagaggc atttttcatg tagaagcggc tatcaaaagc cgtggcgaaa cgtccgccgc
    18121 cgtattcgta ccagatacca aactgcatga aagaggtggt ttcatcgtca gcgtggtcag
    18181 ggcgatgttt ggcgtaggtg taagcggtgg agagataaac gccctttaac gcttcataca
    18241 tggcgctcac catcatccca ctttcacttc ccggtacgtc cagcccgtcg gcgcgtccgg
    18301 tatggtgcca ggcgcgagcg gcaaagttag gagttagcag gcccgtgaga tagagctgtc
    18361 ctgtgccgtc ggtgatttcc agaccgttca tccaggtgat gccttcggca aggtcatact
    18421 ggatgtaacc gttcatcatt gccggatagg tgtatttgtc gtcatagcga tcgtacttgc
    18481 caaaatgtag ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat
    18541 aacgaaagtt gccgcttaag ttctgcacct tcagcgcggt cataatgtcg cgggtattgg
    18601 gaatgacgta gcccagatcg ggggtgaagt cgccccacca ttgcaggtca tccagcgagg
    18661 aatcctgacg cagactgagc atgatttcgg tgccatcatt ggtacgataa ccgccataga
    18721 aacggttaat tcctccctcg aaaccacccc agctatggtc tggcacccag gcattgccgt
    18781 cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag gagccgtact
    18841 tcatctccag gccaccttca atataggtgc cgtcgctgta tttatgttta ttctcaccct
    18901 ctaaatccat atatccacca acccctaatt cgccgtaaaa acgaaatgaa ggagaagaca
    18961 tcgaggtaat ggcgggcgtt tttgtgtcgt catgttctgc cggacgtact tcttctcctg
    19021 ccgcagaaaa ggcgtagcaa aacggcagag caagtaatgc tctgcatggt gcagtgatta
    19081 atttcattgt gttaatccat gagctaaaga aaaaatacag ccttttatcg ttgtaattat
    19141 aaaaatataa aggcagaaac tattttcccc ctccccatcc aggaagaggg aaaatgcctg
    19201 ctcatttttt attagtctgc tcaatcaaca ttgctggctg gcgtcgcttc aatatggcgc
    19261 tcttcagccg ttaatccctt tatatattta ctgtatttcc accacgcaaa gccgaggaag
    19321 ataacaatac cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat
    19381 gtcgaagcga caaaccctac ggcaaatatc gcaatcagca ttgaaacaac gataataccg
    19441 gtgcggcggg agcccatgcg gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc
    19501 aggtaagcca gcatgatgaa taacggcgga agcatggacg ctgcggcagt catattaata
    19561 atagtattca tcagatcctg cacggtattg gaaccgagca tcgggataat catcagcggg
    19621 atgacgatca ggaactggat ccacgctgcg cgcgccggaa cgccgttttc gttcagttcg
    19681 acggttttct taccaaagat gccttccggg atttcggaga agaaaatttt caccggcgtt
    19741 gcggtccaca tcagcaggga accgaacatc gcggtaaagg acaccagacc gacaaagcga
    19801 ttcatcaacg cttccggtag accaaaatac gccgccatgc cgtggaatac ctgcaccgat
    19861 ccgccggtaa atttcaactc tttgctgctg acgaagacgt taatcagcac cgaggagacg
    19921 gaatacagta cgccgataaa aatcccggcg aggatgatca ctttaacgaa cgatttcgaa
    19981 ccgcctttga cgtcgttaac gtacacagcg acggactccg cgccacctgc ggccataaag
    20041 atccaggtgg taacgccgag gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg
    20101 atggggtcag caggctgtac gccgccaacc agcgccgtac ctgccagtaa aatgtaggag
    20161 agcgttaaca gcagcatcag cgttgaagtg acggaggtaa ttggccccaa cattttcgcc
    20221 ccgttggtgg aaacccaggt ggagaaggcg aacagcacca tactgataat ggtggtggca
    20281 accggcgtca taatatattc gtatccgagg aaggcatacg aagcataagc aataacgcgc
    20341 ggcaacagtg aggtgaaaaa gaacaggttt acgaaccagt aggtataggc agtaataaat
    20401 gcccaacgtc cgcccagcga actttttacc cacgcgtaga caccggcttc tgagttttta
    20461 tttaacgaaa caaattctgc gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg
    20521 aggaaaaaca tcggtaccga ggccagtcca agctcaatat tattattgat aacgttgtta
    20581 aagctgtaaa cggcggcaaa agtcagcgag agcaagccga atttgccgat tgtattacgt
    20641 ttggtatcag acataacaca tctccgaaaa tgtgtagggt agcggtggcg gcaaactcct
    20701 tttgccgccg tagttgttat ttgttatgga aataaccatc ttcgatggtg actttgagaa
    20761 ccactttttt gaccgcgtta ttgcagataa accgatacgc ttcatggata tcgcaaataa
    20821 cgatttgccc ttcgtggacc tcaacggttt ctccgcagcc ttttaaatat tcacggtcag
    20881 tttcatcacg ataatattcc actacctgta atgtctcttt cggtgcatat tcaatttttt
    20941 gctgcccttg caggtagtaa tgcacttcaa aatagcgtcg atgcccggta aatagcgcat
    21001 cggtagcgga gtctgtctcc acgcggtaag tcaatgagtc accgatggag tgggcgacgc
    21061 caggctgaat gttgtcgata ttttcaatcg cttcaacgca gcgttgccac ttcttgccag
    21121 aggcgtaaat ctggcggaac tgttctaagt tatcgatgat cctcattgct tattctcgct
    21181 gtgcaaattc gtggaaaaga accctgcgcc gaactcatac gacgccaggc tttgcgcggt
    21241 agcttctccg ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac
    21301 gcgccaggag tccagcacct cgctgcccca ggagttggag ccgaggccaa gcagctggtg
    21361 atcgaggttc agggtgatgt catcactgcg ctgcagctcg ttacagtgct gggcagcgtg
    21421 gatgttttcc tgggtatagt gccaggcgct gaagttaatt gggcgctgcg gaaccaccag
    21481 cagaccgtta ccgtggcggt tagtcagtgc cgtccagcgg acatgctgac ggttactgtt
    21541 gttctgcggg aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat
    21601 gatgttagcc tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac
    21661 ctgatcgtat tcgccgttaa ttcccatggt gaaaccgatg cacggaatga tgtgcggata
    21721 gtcgccgtaa cgctcgccgg aaagcgccac gttaacctgg ccatcggcag cgatgcgcca
    21781 gatgtaggtg cagcgcatcc cgaagtcaaa caccggcggg gcaataactg tgcggctgat
    21841 gatcagcact tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat
    21901 ctgcaaatga ttcggttgcc acagcccttc gtactcctgc ttgtggttgt cgatcatcgg
    21961 cttgaagaag ttgatctttg gctcgcgagt cagcagcgat tcgccattca cctgccagga
    22021 tgtcggtttg ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc gaacggtgca
    22081 gctcaaacga tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga aaggcactgg
    22141 ctgcgcggtg ttttccttca gcgggaactg ataagtggcg atagaatgcc cggcttcgct
    22201 gtagcgggtg cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag
    22261 ctgcggcagc gtgatctgca agggggcttc gctgttcggc gcaacgtcgc gcagtttaat
    22321 ctgctgcgtc gcgagcgttt caccttcgac gcgcacctct gcgtgcaggg tgtagtcatc
    22381 aagcgtggta aaccacagtt tattttcgac tttcagctcg ccgtgagtca gatccagcgc
    22441 gtggattttt accggcgcga taacctgttt gtactctttc aggcccggtc ccggcgtctg
    22501 atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt cgccgtagtc
    22561 gccgccgaat ttataccaga cgttgccgtt gtcatcctgc gcctggatcc cgtggtcgca
    22621 ccattcccag acataatgtc cctgaatgca atcgtgctta tagaagacgt tctggtactc
    22681 cgtcagcccg cctggtccgt tccccatcgc atgagcatat tcacagatga tgcgcggctt
    22741 cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca tggtggaaat
    22801 aatatcgacc acttcagcat cgcgatcttc ttcgtaatgc accagtcgcg tgtcatccag
    22861 cgccttcgct gcgtggtaca tcgcgcggat gttacagcca tagccggatt cattgcccag
    22921 cgaccagatg atgatcgacg gatggttttt ctgcgcgtga atatggcgaa caatgcgctc
    22981 gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc
    23041 aaagccgtgc gattcgacgt cggtttccgc catcacaaac aggccgtaga tatcacacag
    23101 ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga tattgtgctg
    23161 cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc ctttgcgatg
    23221 atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga tccagaacag
    23281 accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt
    23341 gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt ctgctgacca
    23401 ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt caattgccaa
    23461 atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacagggtat attccagcgt
    23521 cgtgacgaca ggggaggcgg cgagattttc cagcaccact tcgcaggaaa gcgtggcatc
    23581 gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt
    23641 tccgaccaga taaacatcgc ggaagatccc cgctgaccac cacatatcct ggtcttccac
    23701 gtaggtagag tccgcccact gcatcacgcg cacacacaac aggttgtcgc cggttttaac
    23761 catcgcgctg atgtcaaact ctgcggtcag gcgacggccc ttgctgaaac ccacatactg
    23821 accgttaaca tagacttcaa aataggtttc gacgccgtca aatttaatca gcgtctgttt
    23881 accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg ggttatcgct
    23941 ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc
    24001 gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag ccattaactc
    24061 agaggtgaag gcttctggta cttgcagcgg atggtcaaaa aagtggaaat tccactgacc
    24121 gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt gcgcaacaga
    24181 atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt
    24241 ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca tggtgatggt
    24301 agtttgtgta attgctgggg agtttggtaa aatttttact aaaaagtagc aggtaaatgc
    24361 atttacttta actggatcac gaaaataaac ggcagcactg tccggcaaaa acgattgccg
    24421 gacagtgtgt aaggggattt aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa
    24481 gactaacagc ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt taacgccctg
    24541 acttcccatc atctcggaat ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc
    24601 ggtggggata tcgttaacgc tgataagcga aatatcctgt gggatgttca ggcctcgttc
    24661 atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg
    24721 atagtcttcc cgcgccagca tttgttttgc cagttcataa cccgacgaac tggaaaaacc
    24781 gccgcgccag atatcctctt cgcgtaccac ttgtttcagt cggccatatt ccgcaaaggc
    24841 gacctcacga atatccgcct tgccaagctc atcttcaccg ccaataaaac caatacgatt
    24901 aacgccctgg ttgatataga agtcgatgat ttctttactg atgcgtgcca gatcgatatc
    24961 caccgcatcg taaccgctgc cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa
    25021 cgcgctggca gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa taccggtgac
    25081 gtttttaatg tctggtaagc cgctgtgttc ataacagttg gtgagctcga tgcccagctt
    25141 ttcgcactgg gtttcaatgc cgtggcggat cgccagatag taaggatcgt tgatctccag
    25201 ctcctgctgg tagctgtaga tagccagaat atggtgttgg ttgactgcac ctgtctggag
    25261 tttacgggca ctactggtct tgtactccag cttttcggcg atctcgagaa tgcgatgttt
    25321 cgtctcttct ttcacattca atgtcggatc gtcatttaag accctggata ctgtcgccag
    25381 ggatacgcca gcttcgattg cgatgtcttt tagtgttgcc atgtttacct tttttacttt
    25441 ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat
    25501 aaagcgcgtt tctttgacgc tgtaacggta acacagcggc tcacgaatgt gatcacgcct
    25561 gcattctcca tttttagcct acaggattga gaaaaacttt tgctatagtt tagtaaaatt
    25621 ttacccgaag ggaggaaaca atggaaaccg tggcttacgc tgattttgca cgtctggaaa
    25681 tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag ctgtacatcg
    25741 tacaggttga tgtgggggaa aaaacactgc aaaccgtgac cagcctggtg ccgtactaca
    25801 gcgaagaaga gctgatgggg aaaacggtgg tggtcttatg caatctgcaa aaagcgaaaa
    25861 tgcgtggtga aacgtcggaa tgcatgctgt tgtgtgcgga aaccgatgac ggcagtgaaa
    25921 gcgtgttgtt aacgccggag cggatgatgc ctgcgggcgt acgcgtcgta taaaaagatc
    25981 ggatggcgac gtcgtatcgc catccgattt gatattacgc ttcttcgaca cttactcgca
    26041 tggccgccag cgccttacgc gccgctttga tcaccagttc acactgttca atggtcagtg
    26101 tcagtggcgg ttcaatgcgg atcgttttgg cgttattgag cgttccggcc accagtacgc
    26161 gctggcggaa catctcgctg gcaaagttat agccgatttc gttatcaaca aactcaattg
    26221 ccatcaacat ccctttacca cgcgcttcct gtaccagatc gggatattcc cgcgccagtt
    26281 gacggaaacc gtccagcaac atatcgcctt tttgctcagc ctgagccggt aagttctgct
    26341 ccagcaacac attgatggtt gccagcgccg ccgcacaggc cagcgggttg ccgccaaagg
    26401 tggtggtatg caggaatggg ttgtcgaaca gaactgaaaa cacctcttca gtggcgatgg
    26461 tcgcgccaat cggcatcacg ccgccgccga gcgctttggc aaggcagagg atatccggct
    26521 gtacgttctc atgctcgcag gcgaacatct tgcccgtgcg ccccatgccc gtttgtactt
    26581 catcgaggat catcagtgcg ccgaactcat cgcatagctt acgtacggcg gtgagatagc
    26641 ccggcggcgg cagaattacg ccaccttcac cctgaatcgg ttcgaggatc accgcagcca
    26701 catcatcacc ggtttttttg cactcgttaa gagccgtgcg catggcttcg atattgccaa
    26761 acggcacatg acggaagccc ggcagtaacg gcataaacgg tttgcggaag gtcgatttcg
    26821 ccgtggccga cagcgcgcca agtgatttac cgtggaacgc gccgctggtg gcaataaaag
    26881 taaacttgcc gcgcggtgac tggtaagctt tcgccagctt cagcgctgct tcaacggact
    26941 cggtgccgct attacagaag aagctgtatt tcagtttacc gggcgttagc gcagcaaggg
    27001 ttttcgccaa catcgcccgt aacggatcga gcagctcctg gctgtgcagc ggttgtttcg
    27061 caagttgatt ctgtacggcg gaaaccacaa ctggattacg gtgccccacg ttgaaaattc
    27121 caaaacctcc caggcagtcg ataaactcct gtccctgggt gtcgacaagc gtatttaaac
    27181 ttcccgcttg ccactctacg gctccgtaat ccccgccggc ggtaacagat ttgcgatact
    27241 ctaaaaaccc cggattgaca tgctctttga agtattcaat cacctctcgg ttaagtgctt
    27301 tcatctcctc atgatccagc gttcgctttt caatgagatt cagggcgtgg gcgctgcacg
    27361 ctaaagccga tgcgctcgaa ggtaacctgt tcaaaatatg ctccggaggc tcgcgtatca
    27421 catgataccg atttaagtat tgcagggatt gcgccactcc ggctgcgatc gcgaaaatcg
    27481 ggataaacac aaatgtggtt atcatttgcg caatatttaa tgctatttgt taacatctgt
    27541 tgttattttg cacggttttt gtcgagtatg gatttgatga ttgctgcgca aatgcacgtt
    27601 ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt atagtaacag
    27661 agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat tgcgatctaa
    27721 atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg acaagaagtt
    27781 aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg tcacccagca
    27841 aaataccccg ctggcggacg ataccactct gatgtccact accgatctgc aaagctatat
    27901 cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag agttacaagg
    27961 gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg cggatatgtg
    28021 gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc gcaaaaatgg
    28081 cgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa aaatcagtgg
    28141 ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg agccgctgta
    28201 caaagcgctg aacgccggac gtaccagtaa gcgtattcat aaaggcctgg tggtgcgtaa
    28261 aggctggctg ggtaaactgc cttcattacc gcttcgctgg cgggcgcgtg gagtgatgac
    28321 cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg tggtgacgta
    28381 tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc agattgtgcg
    28441 cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc gtaatagtgt
    28501 tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag ggcagcttgg
    28561 cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg tcagaaatgg
    28621 cagtgagatg ctggcgaaag gcaccgatga actgaacaaa catacccagc agacagttga
    28681 taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga aacagaactc
    28741 tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg cagtgcaggg
    28801 tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca gtacccagcg
    28861 cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata ttctggccct
    28921 gaatgcggcg gtggaagcgg agcgtgccgg cgaacagggc aaaggttttg cagtggtggc
    28981 aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata ttcgcaagct
    29041 gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg ccgccggacg
    29101 gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg cccagattag
    29161 ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag tggatgagct
    29221 taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg tgtcggcgat
    29281 ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt aatcggtatg
    29341 ccggatccgg cggttggagc acaatgcctg atgcgatgct ggcgcatctt atcaggcctg
    29401 cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc cggcaattgc
    29461 accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa gcggcgcggt
    29521 cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg ttgacgcgta
    29581 aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc tggcgcagcg
    29641 ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt tcgcggtttt
    29701 cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca ccttcctctt
    29761 cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgttgggtag atgacgcccg
    29821 ggcttggggt gtaattcccc tgggttaggt tctcaatcgc tttaatcaat tcgtaaccgt
    29881 ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg tgaccaaaga
    29941 aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcagcattga ccgtgttcat
    30001 gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag cagccctcat
    30061 ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata tcagcctcct
    30121 tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca agtacaaaaa
    30181 tgatttttaa acgtggaagc acctcacatc taggccggat aagacgttca cgccgcatcc
    30241 ggcagcattg ctatggcact gaatgccttc acatacttca gatatatcag ttctacaaat
    30301 cgcttgcatt tatcatgatt aacaatcatt atcatttacg aaaattattt agatatatct
    30361 gattactatc acgaaggcga taacaatgaa taactccccc cgctacccgc agcgcgttcg
    30421 caatgatctg cgcttccgtg aactgactgt gttacgcgtt gagcgcatca gcgccggttt
    30481 tcagcgcatt gtcctcggcg gcgaggcgct ggacggtttt acatcgcgcg gctttgacga
    30541 tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa cggtaacgga
    30601 agagggcatc gtctggccgg aaggaccacg cccaccgtcg cgtgactata cgccgctgta
    30661 tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg gggtcgccag
    30721 cggctgggcg atgcaggcgc aaccgggcga taaacttacg gtggcaggtc cgcgcggttc
    30781 gctggtggtg ccggaagatt acgcgtatca gctgtatgtc tgcgatgaat ccggaatgcc
    30841 cgcactgcgc cgccgcctgg aaacgttgag caaacttgcc gttaaaccgc aagttagtgc
    30901 gctggttagc gtgcgggata acgcctgtca ggattatctc gcgcaccttg atggttttaa
    30961 tatcgaatgg ctggcacatg atgagcaggc ggtagatgcg cgtctggcgc agatgcaaat
    31021 ccctgccgat gattacttca tctggataac cggcgaaggt aaagtcgtta agaatttaag
    31081 ccgccgcttt gaagcggaac agtatgaccc acagcgggta cgtgcagcgg cttactggca
    31141 cgcaaagtaa ctgatatata aaaagaaaaa ggctgacgat ttctcgtcag cctttgctat
    31201 atctggtggc ccctgctgga cttgaaccag cgaccaagcg attatgagtc gcctgctcta
    31261 accactgagc taaggggcca tggtagcgga ttataaagta actccgtgtc gcaatctagc
    31321 cattaccgtg cgcctgctgt ttttataaac aatgtatttt caatccgtta tacttttcct
    31381 ggtgatgtta gaaaggagta aatatggttg aggatatttt ggctccaggg ttacgggtcg
    31441 tgttttgcgg tatcaaccct gggctttcat ccgccgggac tggttttccc tttgctcatc
    31501 cagcaaatcg cttctggaag gtgatatatc aggctgggtt taccgaccgt cagttgaagc
    31561 cgcaggaggc acagcatctg ctggattatc gttgtggcgt caccaaactg gtagaccgtc
    31621 caacggtgca agccaatgaa gtttcaaagc aggagctgca cgcaggtggg cgtaagctga
    31681 ttgaaaaaat tgaagattat cagccgcagg cgttggcgat tctgggcaaa caagcatatg
    31741 aacagggatt cagccagcgc ggtgcacagt gggggaaaca aacgctcacc attggttcga
    31801 cgcagatttg ggtgctgcca aatcccagcg gtttaagtcg cgtttcacta gagaaactgg
    31861 ttgaagcata tcgcgagctg gaccaggcgc tggtagtgcg tgggcgataa aaaacgccac
    31921 cgaaaaggtg gcgtttgtgc agagggacta gtgccgggtg cggcgtaacg cctgatccgg
    31981 cctaccgatt aatcgtccag gaagctacgc aacacttcag aacggctcgg gtgacgcagt
    32041 ttgcgcagcg ccttcgcttc gatctgacgg atacgttcgc gggtaacgtc gaactgttta
    32101 cccacttctt ccagcgtgtg gtcggtgttc atatcgatac cgaaacgcat acgcagaact
    32161 ttcgcttcac gcgcggtcag gccagccagc acgtcgtgcg ttgccgcacg caggctttcg
    32221 gtggtcgcag aatccagcgg cagctcgagg gtggtatcct cgatgaaatc ccccagatgc
    32281 gaatcttcat catcaccgat cggcgtttcc atggagattg gctctttggc gatcttcagc
    32341 actttgcgga tcttgtcttc cggcatcagc atacgttcag ccagttcttc cggcgtcggt
    32401 tcacggccca tctcttgcag catctggcga gaaatacggt tgagtttgtt gatagtctca
    32461 atcatatgca ccggaatacg gatggtgcgc gcctgatccg cgatagaacg ggtgatcgcc
    32521 tgacggatcc accaggttgc gtaggtggag aacttgtaac cacggcggta ttcgaattta
    32581 tcaaccgctt tcatcagacc gatgttgcct tcctgaatca ggtcaaggaa ctgcaagcca
    32641 cggttggtgt atttcttggc gatagaaata accagacgta agttcgcttc aaccatctct
    32701 ttcttcgcac ggcgggcttt cgcttcaccg atggacatac gacggttgat atctttaacc
    32761 tgctcgatgg tcaggccggt ttcttcttca atctgctgca gtttctgcag ggcgcgatgc
    32821 acttcttcag agacatcgtg cagtttttcc gaccacggct tgttcatcgc aattgccgcg
    32881 ttgaaccagg tatcgctggt ttcgttgccg gtaaacaggg taatgaagtt tttcttcggc
    32941 attttgcact gctcaacgca gagcttcatg atcagacgtt cttgcgtacg aacgcggtcc
    33001 atcatgacgc gcatgctgtt gaccaggtag tcaaactgct tcggcaccag gcggaactgt
    33061 ttgaatactt cagacagttt caggatctct tcctgagcgg cagcgtgact gcgacctttc
    33121 gctttgatgg tgtcacgcgt tacaacgtac tgagcgcgta gttccgcaaa tttttcgcga
    33181 gccagttccg ggtcgatgct gttgtcatca tcggcgctgt cgtcatcgcc atcttcttcg
    33241 tcttcatctt cgtcatcgtc cagatcttcc tgggaaagct cagaaccgac gtgagtggcg
    33301 gtaggtgcca gatcttcttc tgcgttcggg tcaacaaagc cggtgatcag atcggacaga
    33361 cgcgcttctt ctgcttcaac acgatcgtac tgttccagca gataggtgat cgcttccgga
    33421 tattcagcaa cggagcattg aacctggttg atcccgtctt caatacgctt agcgatgtca
    33481 atttcgcctt cgcgggtcaa cagttcaacg gtgcccattt cacgcatgta catgcgtacc
    33541 gggtcagtcg tgcgcccgat ttcagattcc acgctggaaa gcacctgcgc ggcggcttcg
    33601 gcagcatctt cgtccgcggt gttttcagcc agcatcagat catcggcatc cggtgcttct
    33661 tccatcacct gaatgcccat gtcgttgatc atttggatga tgtcttcgat ctgatctgaa
    33721 tcgacgatat cttccggcag atggtcattg acctcggcat aggtcagata gccttgctcc
    33781 ttaccacggg tgacaagaag tttcagctgt gactgcgggt tttgctccat aagacggtat
    33841 ccacacttat ttcatgaggt tggtgttggg cgattgaccc gatgtccttc agccgttaac
    33901 gctgaaatcc aacggttgtt taccgccaac gataattacg agggcgtact tatatatttg
    33961 ccgctgcctc tcagtgcggc tgtcgggggc ttcccgatcg ctcttcggca cttaagccgt
    34021 taaatcactt tttcgccagc tcctggttta atgtccagag ctccaggcgt tcttcgttgc
    34081 ttaaaccatg cgtgcgctca cgagcgatta actcttcctg gcgcagttca agcagcgaat
    34141 caaacatatg gttgagtgag tcggtgaagg tttgctcagc aatattctta tctgctatat
    34201 cgtcccacat cgacagtttt tcaagggtgg cagcattatt tgtaccacga tagtgctcta
    34261 aaagttgccc ggtggtcaga cctggctggg agagacaagt gttgaccagt tctctgaata
    34321 agccaagtcc agggagctta ttttcatcca gattctcaag cggcgggacc aacgtcgcta
    34381 attctggatt ttgcaccagc aaccctataa gtatacgcat ggtcgtgcgt tttagctgcg
    34441 gaacagggcg agaaacgccg ctctctgccg cttttggcat taatcgttca agctggctgt
    34501 catcaagtat gcctaatttg ttgcctaatt cctgacgaag atatattcgc agcgtttcgc
    34561 ccggcacttg cgatatcaat ggcagtgcca gcgtactcaa acgtgcgcgc ccgtcagggg
    34621 tactcagatc aacttgcggc atcaggctgt taaacagaaa tgcggagagt ggcatcgcct
    34681 gatccatccg cgcttcaaac gcttctttac cttcttttcg taccagcgtg tcagggtctt
    34741 cgccatccgg caaaaacata aagcgtagct gacggccgtc tgtcatgtaa ggcagcgccg
    34801 tttccagcgc tcgccatgcg gcatcgcggc ctgcacggtc gccgtcataa cagcaaatga
    34861 cattgttggt cgcgcggaac aacagttgta tgtgatcggc ggtggttgac gtacctaacg
    34921 acgcaacggc gtaattaatg ccgtattgcg ccagcgccac cacgtccata tagccttcga
    34981 ccacaagcag acgattgggt tcagcgttat cctgctgcgc ttcataaaga ccgtaaagct
    35041 ggcggccttt atggaaaatg tctgtttccg gcgagttcag gtatttgggg gtatcgttgc
    35101 ccagcacgcg cccgccaaaa ccaatcaccc gaccgcgttt atcgcgaatg gggaacatca
    35161 cccgctcgcg gaaacgatcg taactgcgtc cctgatcgtt agtgaccaac atccccacat
    35221 caatcaatga ctggcgattt tctggattgc cgccaaaccg cttcaggacg ttgtcccagc
    35281 cggggggcgc aaaaccaatc gcaaagcgag cgataacctc gtggcttaat ccgcgttttt
    35341 ccagatactg gcgcgcagac gtggcaacag gttgttgtaa agattgttgg taaaacgtat
    35401 tcagaccgtc catcaactga taaagcgttt gcctctgatg gcgctcgatc tggctggggc
    35461 cgctgcctgc ttcaaatggc acttcaagat tgtgcattgc tgccagctct tcgaccgttt
    35521 caacgaactc gagcttgtcg tagttcatca ggaagtcgat cgcgttgccg tgcgcgccac
    35581 atccaaagca gtggtaaaac tgtttctcac cgttaacggt gaatgacggg gttttctcgt
    35641 tgtggaatgg acaacacgcg tggaaattct tgccctgctt tttcagcttc acacgggcat
    35701 cgatcagatc gacgatgtca gtgcgtgcca gcagatcatt aatgaatacg cgtgggattc
    35761 gtccagccat aggccccgat ttttagcaat tcataaacga aaataagccg cgcattcctt
    35821 tcggaagcac ggccttacaa ctacaactcg gtctgatcgg agagcaacgc tctcggggaa
    35881 ttagtacaga cgagtgcggc gtgcgttttc gcgagccagt ttcttcgcgt gacgtttcac
    35941 tgcagaagct ttagcgcgct tacgttcggt agtcggtttt tcatagaact cacgacgacg
    36001 aacttccgcc agaacacctg ctttttcgca ggaacgcttg aagcgacgca gagctacgtc
    36061 gaacggctcg ttttcacgta ctttaattac cggcatgtgc ctctcacctt tgattaattc
    36121 ggtttgccgc tggcatcaac gccagcttat ttcaaaatgg tgcggaattt tactgcaatt
    36181 gctgctgctt tgtaaagcac cgcggccttt tttgaaaggg acttttataa gggtgaggag
    36241 tatacacgag gctttctcca ggggcgaaca aagttttaca tcaacccgca ttggtcctac
    36301 actgcgcggt aataaagcga ggtaaaacaa gtcatgcgtg tactgggtat tgaaacttcc
    36361 tgcgatgaaa ccggcatcgc catttacgac gatgaaaaag gtttgttagc caaccaattg
    36421 tatagtcagg tgaaattgca cgctgactac ggcggcgtcg tgcctgaact ggcctcccgc
    36481 gatcacgtgc gcaaaaccgt accgttgatc caggcggcgc taaaggagtc tggtttaacg
    36541 gcaaaagaca ttgatgctgt ggcctatacc gcaggccctg gattagtcgg cgcactgctg
    36601 gttggcgcaa ccgtggggcg ttctctggcg tttgcctgga acgttccggc aatcccggta
    36661 caccatatgg aagggcatct gttagcgccg atgctggaag ataacccgcc ggaatttccg
    36721 tttgtcgcgc tgctggtgtc cggcggtcat acgcagttaa tcagcgtgac tggcattggt
    36781 cagtacgagc tgctcggcga gtctattgat gatgccgccg gtgaagcgtt tgataaaacc
    36841 gcgaagctgc tggggctgga ttatcctggc ggaccgttac tgtcgaaaat ggcggctcag
    36901 ggtactgccg ggcgctttgt tttcccgcgt ccgatgaccg accgtccagg gctggatttc
    36961 agcttttctg gtctgaaaac ctttgcggcg aacacgattc gtgacaacgg caccgacgac
    37021 cagacgcgtg ctgatatcgc ccgcgccttt gaagatgcgg tggtcgatac gttgatgatt
    37081 aagtgtaagc gagcgttgga tcagacgggc tttaagcgac tggtcatggc gggcggcgtg
    37141 agtgctaacc gcacgttacg ggcgaagctg gctgaaatga tgaaaaaacg ccgcggcgaa
    37201 gtgttctacg cgcgtccgga gttttgtact gataacggcg cgatgatcgc ctatgccgga
    37261 atggtgcggt ttaaagcagg cgcgacggcg gatctcggcg ttagcgtgcg tccgcgctgg
    37321 ccgctggcgg agttaccggc tgcgtaaaac tattgggtgc cggagagcaa tttccggcac
    37381 cgtcctcact taaagcaaca ccacgggcat ccacagtaag ccggtaatca ccagcaacac
    37441 gaggaatatc agcccaaaaa tcgcccccag tcgccagtaa tccgccgttg gcagataacc
    37501 actaccgtag taaatcggac tgggtccggt ggcgtatggt gtaagaatgc tccccagacc
    37561 aattgccgcg ccaaccatca ggcagaacac cggcagcggg atttccggca tcgccagcgc
    37621 agcggcaatc atcatcggtg cgagggcgga ggtatacgcc gtggcgctgg cgaaaaagta
    37681 gcgcagcaga taaaacacca caatcaacgc caccatcacc atcgttggcg aataaccgct
    37741 taagctgcct gctaacagtt tgccaaacca gctaataaaa ccggtgttgt tgagtccggt
    37801 agcgagggtg ataagcgagg ccagccagaa gaaaacgttc cacgccgctt tattactgac
    37861 aatgtcgtcc caactgataa tgcgcagaag cagcatcagt gccaccacgc tgtaaccgac
    37921 catcgcggca tcgatataat caccgccgaa aatccacagc accagcgcgc ctaccatcag
    37981 ccccagcatc cgtttttcac gcgaacagag cgggcccatt gcctgcagtt ccgtctctgc
    38041 ccagcgcggc acctgatcgc cagacttcag taccggcggg tacagcacat aagccagcca
    38101 aggaaccagc agcaccagca aaatactgag cggcaacatc ccgaggaacc agtcgcccca
    38161 actcagcgtg gcgtgagatg cgcttttcat cagtccaatt aacagcaagt ttggtgccat
    38221 cgccgtcaag aaaatggcgc tggtcacgca gtcggcaaca atccccatcc acatgatgta
    38281 cgagccaatg ctgcgcgaac tgctgtcgtt tggttgtgat tgatagagcg gtggcaggtt
    38341 acggatgatg ggatagataa tccccgcacc acgcgccgag ttggacggtg ttacaggtgc
    38401 gaggatcagc tcggagaaca tcaccgcata gccgagaaac agcgtgcgat gtcccatctt
    38461 tttcaccaga atcagcgcaa tgcggcgtcc aagcccggtt ttttcatagc ctgtgccaaa
    38521 cataaaggcg gcgaaaatca gccagataac cgaattagaa aaaccggaaa cggcccacga
    38581 gagggatttt gcagtgaatt taaagcctgg ctgggcgagc tgttcggggc tgaacagcag
    38641 ccagggagag agtatggcga tgatggagat acccaccatc gccaccacgg cacccggcac
    38701 gggttcgagg atcagtccaa cgatcacgcc agtaaaaacg gcaaagtaga gccaggtatg
    38761 actctccagc cctgcgggaa ccggaattag agcaataatg gcgatgaccg ccagcggcgc
    38821 aaggtatcgc caccattcag tggaaggttt catcgtgtta tgcctgcgaa aaaggagagg
    38881 gggcgagcct ctccgggagg gttatttgat gtaatggacg tgctcgcaga tctcttccac
    38941 gatgggatcg cggcgttcgg cgaacagctt tttgttttcg gctatcaggt tgttgccgtg
    39001 ggtatcaata gagacaatca gcgggccgaa ctctttgacc cggcagaccc acagtgactc
    39061 cggcatcccg agttctgtcc agtgcacttc ttcaatctct tccacctggg ttgccgccag
    39121 caccgcgcag cctgccggga aaatcacatg tagcgccttg aatttctggc agccttcttc
    39181 ggtcagcggc cccattccgc ctttgccaac caccagtttc acgccggtct gctcgataaa
    39241 ttcacgttca aaactttcca tacgcatact ggttgtcggg ccaacggaaa ccatctccca
    39301 tttatcgccg tttttgcgca cgatggggcc agcgtggaaa atcgctttgc cgttgagatc
    39361 gtaagggatc ggacgtttca gttcgatcaa gcggcggtga caaacgtcac ggcaggtcac
    39421 cagcgtaccg gtcaggtaga tcacatcgcc gacgcgaata tcttgcagat cttcagcttt
    39481 gatcggggtt gtcaggatct ttttcataac gcgctccggg tgtgagacag attttcaaag
    39541 gtgagatcgg catgaaccag cagtgtgccg cgacgatgcg cccagcagcc ggtagagaca
    39601 gcaacgccga tggttgacgg atggcgggcg gcagattcga tatgtacgcc catcactgaa
    39661 ctgttgccag tcagcccttg tggaccaatc cccagacggt tgagtccttc ttccaggcgt
    39721 agctccagtt ctgccgcttt tggattggga tggcggctgc caatcgggcg caaaatggct
    39781 ttacgcgaga gtacggcggc ggtttccacc gaggtggcga tgcccacgcc caccagtacc
    39841 ggtggacagg cgtttacggc gagggtggag atattttcga agacgaattt caccacgcct
    39901 tcgtagcctt ctgacggcat taacactttc gagcggccag gtagcgtgca gccgcgcctg
    39961 ccatgtaaac ttcgatttcc gcatcgtcat tgtcggggat gatgtcccag gtgacccacg
    40021 gtacgccgct accggtattt ttgccggtgt ttacttcgtc aaaaatttct accgcattgt
    40081 gacgtagtgg cgctttgacg gttgcctctt ccacggcttg tttgagtatg ctttgcagct
    40141 cgccaagcag tgggaagcgg gaaccgactt taacgaagaa cataatctcc ccggtgtcct
    40201 gacaggcagg acgattcagg tcaatcgctt tttgcatgtt gtcgaacatc gtatggtaga
    40261 taattttccc catcgacgac gtttcggcat cctttagctg ttttagttta tccaccacgt
    40321 catcaggcat tcgggtagaa atcatggcgg taaagttagc gacaatctct gtcaacttat
    40381 tcactgcctg ttgcttatta ctttcgctca tcatggcaac tccagttttt gtatttgtct
    40441 ggaaaaattc accgccacaa actaccacta aaaatgtggt tttatatttt ttgccaccgt
    40501 gaatatgtaa ttaacagttc gtgaatcaac gcgaaaataa gaatgaatat tgcgccagag
    40561 cagggaatgg caatgttttg cgaaatacac tgcgcttatc acaactgcgg ataattaacg
    40621 atgctgaata gctggcgttt agccaaagac ttgcaggtac tggtagaaat cgtccacagc
    40681 gggagtttta gcgccgcagc ggcgacgctt gggcagacgc ctgcatttgt caccaagcgg
    40741 atccaaatcc ttgaaaatac gctggcaaca acgctgctga accgctccgc tcgcggcgtg
    40801 gcgctgactg aaagtggcca acgttgttac gaacatgcgc tggaaatcct cacccaatat
    40861 cagcgactgg tcgatgacgt cacgcaaatc aaaacgcgcc cggaggggat gattcgtatt
    40921 ggctgtagtt ttggttttgg gcgcagccat attgcgccag ccattaccga acttatgcac
    40981 aattatcctg agttacaggt gcattttgaa ttgttcgatc ggcaaattga tttagtgcag
    41041 gataatattg atctggatat tcgtattaat gacgaaattc ctgattatta tattgcgcat
    41101 ctgttaacga aaaataaaag aatattatgc gcagcacctg agtatctgca aaaatatccg
    41161 cagccacaat cattacagga attaagtcgt catgactgtc tggtgaccaa agagcgcgat
    41221 atgacccacg gaatatggga gttggggaac ggtcaggaga aaaaatcggt gaaggttagc
    41281 gggcatcttt cctccaatag cggcgagata gtgttgcaat gggcgctgga gggcaaaggg
    41341 attatgctgc gttccgagtg ggatgtgttg ccgtttctgg agagtggcaa actggtgcaa
    41401 gtattgccgg agtatgcaca aagcgccaat atctgggctg tttaccggga gccgctctat
    41461 cgcagcatga aattacgtgt ctgcgtggaa tttctggcgg catggtgcca gcaacggctg
    41521 ggcaagcccg atgaaggcta tcaggtcatg tagatccacc agaaatcact cgggatcctt
    41581 ttcgcgcttt cttttgaatt tcgtccagat ttttgtctcc tgacgacgcc acagacgctg
    41641 gatgttgtca tgatgacgca gcaggatcag gcaagagagc atcgaaaccg ggaaggtgaa
    41701 ttgtggctta aaccaccaga cataaaacgg agcaatcagt gcactgacaa tcgctcccag
    41761 cgacgagtat ccgctcaata gcacggtcag taaccaggtt cccgccatta ctccggtgag
    41821 atcccagcca atgggtgcga tggcaccaaa agcggtagca acgccttttc ctcctttaaa
    41881 tccgaagaaa acgggccaga tgtgtccaag acaggcggcg atggcaatta agcctagcca
    41941 gaaggggctg acacctaatt catacgcgcc ccagacgggc aacattcctt tcagaacgtc
    42001 gaaaatcagt actgctacgg ctgctccctt gccaccgata cgtaacacat tggttgcgcc
    42061 tggattgccg gagccgctgg ttcgcggatc gggcagccca cacaagcggc aaaccagaat
    42121 ggcactggaa atggagccgc agaggtacgc gatgaggatc attccaggcg cgattgcact
    42181 cataagctgt tccgttttga aaattcgtgt tcaacgatga atctgtggat aatacgcaca
    42241 tttcgccgga agtggtatcc ggttagccaa aaagcaggca ggacgtgatg gatattgtat
    42301 ttatagagca actttcggta atcaccacta ttggtgttta cgactgggaa cagaccatcg
    42361 aacagaagtt agtgttcgat atcgaaatgg catgggataa ccgtaaagcg gcgaaaagtg
    42421 atgatgtggc ggattgcctc agttacgctg acattgcaga aacggtggtc agccacgtcg
    42481 agggggcgcg ttttgcgctg gtggaacgcg tggctgaaga ggtggcggag ctgctgttag
    42541 cacgcttcaa ctcgccgtgg gtgcgtatca aactcagcaa gccaggcgca gtggcgcggg
    42601 cggcgaatgt tggcgtaatc attgagcgtg gcaataatct gaaagaaaat aattaatttt
    42661 acagctgtta aaccaaacgg ttataacctg gtcatacgca gtagttcgga caagcggtac
    42721 attttaataa tttaggggtt tattgatgag cgatatgcac tcgctgctga tagcggcaat
    42781 attgggtgtg gtcgaaggat tgacagaatt tctgccggta tccagcacgg gccatatgat
    42841 tattgtcggt cacttgttgg ggtttgaggg cgacacggcg aaaacctttg aagttgtgat
    42901 ccagttagga tcaattctgg cggtagtagt gatgttctgg cggcgtctgt ttggcctgat
    42961 tggcatccac tttggccgcc cgttgcagca cgaaggtgaa agcaaaggtc gtttaacgct
    43021 gatccacatt ttgctgggga tgattccggc ggtggtactg gggctgttgt tccacgacac
    43081 gattaagtca ttgtttaacc cgataaatgt gatgtatgca ctggtcgttg gcggtttgtt
    43141 gctgattgcc gccgaatgcc tgaagccgaa agagccgcgt gcgccgggtc ttgatgatat
    43201 gacctatcgt caggcgttta tgattggctg tttccagtgt ctggcgctgt ggccgggttt
    43261 ctcccgttcc ggggccacca tttcaggtgg gatgctgatg ggcgtgagcc gttacgctgc
    43321 ttccgagttc tcgttcctgc tggcggtacc gatgatgatg ggcgcaacgg cgctcgatct
    43381 ctacaaaagc tggggcttcc tgacaaccgg cgatatcccg atgtttgccg ttgggtttat
    43441 caccgctttt gtggtggcgc tgatagcgat taaaaccttc ctgcaattga ttaagcgcat
    43501 ttcgtttatc ccgttcgcca tttatcgctt tattgtggcg gctgcggtgt acgtcgtgtt
    43561 cttttgattg agaaaaggtt cgggagcagg cctggttttc tccctcgctc tgtagggaga
    43621 gggccggggt gggggtgatt gttcgcaccg gtgctggtct gttcccctca ccctaaccct
    43681 ctccccgaag gggcgagggg atggttcgtg ttaacgttta gttttatact cagtcaggct
    43741 ttgggcaacg ttgttccttc caactggcta ccgccgcaat ccgccgtcgg gtcagctcct
    43801 cgcgaatctc cacaccttta aatcccgctt caacgaccgc ttttgtcggc actgactgcg
    43861 ccacttccca ggcttcgcgc aaccaacggc cttgcgggta gtccgctgat tcaaaaccgg
    43921 ttctgccgcg tacgtcagcc tcgctggtca gcgccagttg ctcgacacgc tgcggtttac
    43981 gccaggcatc gatggaatca aataatttga cgatggtttt tgggttcagc attgggaagg
    44041 tgtggatgag atcgtgaaac tcagccacca gtctggctaa atcgcgaatt tcatttggca
    44101 cgcgcagacg ctggcatagt tgttccacta acttaacacc cgccgggcca tgcccatgat
    44161 gacgcggcca aagttctggc ggcgtcagtc ctttaccgag atcgtggcat aaagttgcga
    44221 aacggacatc aacctgcgga ctcagcatcg ccgccattga gagcgtcatt aaggtatgaa
    44281 tacccgtgtc gatttccgga tgccacctgg caggggccgg aacgccaaac agggagtcaa
    44341 tttccgggaa taaaacgcga agtgcaccgc aatcgcgcag tacctggaag aacacctggg
    44401 gattgcgggt ggtgagggcg ctttccgtct ctttccatac ccgttcaggc gtcaggtgtt
    44461 ccagttcacc cgcatgggtc atctcgcgca tcagcgtcag agtttcatcg gcaatacgaa
    44521 aaccgaggtg ggcataacgc gcagcaaaac gcgccacgcg cagaacgcgt aacggatctt
    44581 cgccaaaagc gggggaaaca tggcgcaaca gacgattttg cagatcgccc agaccgttgt
    44641 acgggtcgat aatctcgccg ttatcgtcct gggccagcgc attaatggtc agatcgcgac
    44701 gcttaagatc atcttccagc gtgacatccg gtgcggcata gcaagtaaaa ccggtgtaac
    44761 cggaaccgga tttccgttcg gtacgtgcca gcgcatactc ttcatgcgtt tgcggatgca
    44821 gaaacacagg aaaatcgcgg cctacctgct ggtagcccgc gtcgagcatc tcctgtggcg
    44881 tactgccgac caccacccaa tctctgtctt tgaccggtag ccctaacaat gcatcccgaa
    44941 cagcaccacc gaccagataa atcttcacgc catcatccct tcgtcaaata cacaattcct
    45001 aataataaga cagtgcaacg aagaaggcga tttagttcat ccagcgatct ttgcgtttgc
    45061 gcgttgggat caggtgtggc agtaccagac cgagcagcaa gccaagcccc agcacgccac
    45121 cgccatacat aaaccattgc atgatgatgg tgcgctgttt gtcatccagc tgtacgctgg
    45181 cggcatcgac ctttttctgc gcgacaatca gctcgttttt caatttctga ttttcttctt
    45241 ttaatccgtt gatcacgctg tcgctctgcg ccactttttg ctgcatttct gcggtgcgct
    45301 gattccaggt gttatcgata ttggtgagtt tatcggtcag ggttttgacc tgattttcca
    45361 gatctggcac acgggagcgc aggcttggct cagtgctaag ttgtttcaac gggatccagg
    45421 cggtacggcc agagctgtct ttcacctggg cataattggt gttggcgtca gtttgtaata
    45481 aggtcacttc ctcgccggcg ttaaccgtgc ccacgaggcg ataatgatct cccggaccgc
    45541 tacggaccca ggtattcagt tcatcagaaa catagcgcgt ttcttcagcg tgtgagacgg
    45601 cagtggcgct aagtgcgagt aaagttaatc cgatcaggcg taattttggc atcaggctgt
    45661 cgttattgtc atggaaagtg gaacgatagt agtggcatca gtgtcgctac gcaaagcatt
    45721 cgacatcaat cggaatcctc tgtgtcaggt cttgccactg cttcaaactt ttacgcccgt
    45781 caaattgccc gttgcatggc aatttggcgc aaaatactat ctactgacaa aaaagatcgc
    45841 attaagttcg ccgttcatcg tcacttatgt gacataaccg ataagtaaag gccatggctc
    45901 aggaaatcga attaaagttt attgttaatc acagtgccgt tgaggcgttg cgtgaccatc
    45961 tcaatacgct gggcggcgag caccatgacc ccgtgcagtt gctgaatatt tactacgaaa
    46021 cgccggataa ctggctgcgt gggcacgata tgggcttacg tattcgtggc gaaaacggtc
    46081 gctatgagat gaccatgaaa gttgcaggaa gagtgacagg cggcttacac cagcgcccgg
    46141 aatataacgt ggcgttgagc gaaccgacgc tcgacctggc gcagttaccg acggaagtct
    46201 ggccgaacgg cgaattgccc gccgatctcg cttcccgcgt acaaccgctg ttcagcaccg
    46261 atttttatcg cgaaaaatgg ctggtggcgg tcgatgacag ccgaattgaa atcgctctcg
    46321 atcaggggga ggtgaaagcg ggggaatttg ctgaacctat ctgtgagctg gaactggaac
    46381 tgcttagcgg cgacacgcgc gcggtgctga aactggcgaa ccaactggta tcgcaaaccg
    46441 gattacgcca gggcagcctg agcaaagcgg cgcgtggcta tcatctggcg cagggcaatc
    46501 cggcgcgtga aatcaaaccg accaccattt tgcatgttgc ggcaaaagcc gatgtggaac
    46561 aggggctgga agcggcgctc gagctggcgt tagcgcaatg gcagtatcat gaagaactgt
    46621 gggtacgcgg caacgatgcg gcgaaagagc aggtgctggc agccattggc cttgttcgcc
    46681 atgcactgat gctgttcggt ggcattgtgc cgcgtaaagc gagcactcac ttacgtgatt
    46741 tgctgactca atgcgaagcg accatcgctt ctgcggtttc tgccgtgacg gcggtctact
    46801 ctaccgaaac ggcaatggca aagctggcgt tgaccgaatg gttggtaagc aaagcctggc
    46861 agccgttttt agatgccaaa gcgcagggta aaatcagcga ttccttcaaa cgctttgccg
    46921 atatccatct ttcccgccat gccgctgaac tgaaaagcgt tttctgccag ccgttaggcg
    46981 atcgctacca tgatcagttg ccacgcctga cgcgtgatat tgactcaata ctgctgctgg
    47041 caggttacta tgatcctgtc gtcgcgcaag cctggctgga gaactggcag gggctgcgtc
    47101 acgctattgc gaccgggcaa cgcattgaaa ttgaacattt ccgtaatgag gcaaacaatc
    47161 aggaaccgtt ctggttgcac agcggaaaac gttaatcagg caaggataaa atcgcttatg
    47221 aagccgctct cttcaccgtt acagcagtac tggcagaccg ttgttgagcg gctgccagag
    47281 cctttagccg aggaatcact tagcgcacag gcgaagtcag tacttacttt tagtgatttt
    47341 gtgcaggaca gcgtgattgt gcatccagag tggctgacgg aactggaaag ccaaccgccg
    47401 caggccgacg aatggcagca ttacgcggca tggttgcagg aggcgctcag taatgtgagt
    47461 gacgaagccg ggttaatgcg cgagctgcgg ctattccggc ggcgcattat ggtgcgcatc
    47521 gcctgggcgc aaacgctggc tttggttact gaagagagca tattgcagca gctcagccat
    47581 ctggcggaga cgctgattgt cgccgcgcgt gactggctgt atgacgcttg ctgccgcgag
    47641 tggggaacgc cgtgcaatgc gcagggcgaa gcgcaaccgc tgctgatttt aggcatgggt
    47701 aagctgggcg gtggggaact gaatttctcc tcagatatcg atctgatttt tgcctggccg
    47761 gaacatggtt gtacgcaggg cggacgccgg gaactggata acgcgcagtt ttttacccgc
    47821 atggggcagc ggctgattaa agtgttggat caaccaacgc aggatggctt cgtctatcgc
    47881 gtggatatgc ggctgcgtcc gtttggcgaa agtggcccgc tggtgctgag ctttgccgcg
    47941 ttggaggatt attaccagga gcaggggcgc gactgggagc gttacgcgat ggtcaaggcg
    48001 cggattatgg gcgatagcga aggcgtctat gctaacgagt tgcgtgcgat gctgcgcccg
    48061 tttgttttcc gtcgttacat cgatttcagc gtgattcagt cgctgcgcaa catgaaaggg
    48121 atgattgccc gtgaagtgcg tcgacgtggt ttgaccgaca atatcaaact cggcgcaggc
    48181 ggcattcgcg aaattgaatt tatcgttcag gtgttccagc tcattcgcgg tgggcgcgaa
    48241 ccgtcgctgc agtcgcgctc tttactgcca acgctcagcg tcattgccgc gctgcatctg
    48301 ctttctgaaa acgatgctga acaattgcga gtggcgtatc tgttcctgcg gcgtctggaa
    48361 aacctgctgc aaagcattaa cgacgaacaa acccagacgc ttccttctga tgagcttaac
    48421 cgtgcgcggc tggcgtgggc gatggacttt gctgactggc cgcaactgac cggggcgctg
    48481 accgcacata tgaccaatgt gcgccgggtg tttaatgaat tgattggcga cgatgaaagt
    48541 gaaactcagg aagagtcgct gtcggaacag tggcgtgagc tgtggcagga tgcgttgcag
    48601 gaagatgaca ctacgccagt gctggcgcat cttagcgagg atgatcgcaa acaggtgcta
    48661 acgctgattg ccgatttccg caaagagctg gataagcgca ccatcgggcc gcgaggacgt
    48721 caggtgctcg accatctgat gccgcatctg ctaagtgatg tctgtgcgcg tgaagacgct
    48781 gccgttacgc tgtcgcgcat taccgccttg ctggtgggga ttgttacccg caccacctat
    48841 ttagaattgc tcagtgaatt ccccgcggcg cttaaacatt tgatttctct gtgtgccgcg
    48901 tcgccgatga ttgccagcca actggcgcgt tatccattat tgctggatga attgctcgat
    48961 ccaaacaccc tttaccagcc gacggcgacc gatgcctacc gcgatgagtt gcgccagtat
    49021 ttgctgcgcg tgccggaaga tgacgaagag caacagcttg aggcgctgcg tcagttcaaa
    49081 caggcgcagc tgttacgcat cgccgcagcg gatatcgctg gtacgctacc ggtgatgaaa
    49141 gtgagcgatc acttaacctg gctggcagag gctatgatag atgccgtcgt tcagcaggcg
    49201 tgggttcaaa tggtggcccg ctacggtaag ccaaatcacc tcaacgatcg cgaaggccgt
    49261 ggttttgcgg tggtcggcta cggcaagctg ggcggctggg agttaggcta cagttccgat
    49321 cttgacctga tcttcctcca tgattgccca atggatgcga tgactgacgg cgagcgggaa
    49381 atcgacgggc gacagtttta tctgcgtctg gcgcaacgca ttatgcatct gttcagtacg
    49441 cgaacctctt ccggcatttt gtatgaagtg gatgcacgac tgcgtccgtc cggggcggcg
    49501 ggaatgctgg tgacatccgc agaagcattt gctgattatc agaaaaacga ggcctggacg
    49561 tgggagcatc aggcgctggt gcgtgcgcgt gtagtgtacg gcgatccgca gctcaccgcg
    49621 cactttgacg cagtgcgtcg cgagattatg acgctgccgc gtgaaggtaa aactctgcaa
    49681 acggaagtgc gggaaatgcg cgagaaaatg cgcgctcatc tcggcaataa acatcgtaat
    49741 cgctttgata tcaaagccga tgaaggcgga attaccgata tcgaatttat tacccaatat
    49801 ctggtgttgc gctacgctca tgaaaaaccg aagttaacgc gctggtcaga caacgtgcgt
    49861 attctggagc tgctggcgca aaacgacatt atggaagagc aggaagcgat ggcgctgacc
    49921 cgtgcttaca cgacgctgcg cgatgaactt catcatctgg cattacagga attgccgggc
    49981 catgtgtcgg aggattgctt caccgcagag cgtgatctgg tgcgggcaag ctggcagaag
    50041 tggctggtgg aagaatgaag tatggtatta tcgcgcgcaa attttgaatc tctcaggaga
    50101 caggaatgaa agtaacgctg ccagagtttg aacgtgcagg agtgatggtg gttggtgatg
    50161 tgatgctgga tcgttactgg tacggcccca ccagtcgtat ctcgccggaa gcgccggtgc
    50221 ccgtggttaa agtgaatacc atcgaagaac gtccgggcgg cgcggctaac gtggcgatga
    50281 atatcgcttc tctcggtgct aatgcacgcc tggtcgggtt gacgggcatt gacgatgcag
    50341 cgcgcgcgct gagtaaatct ctggccgacg tcaacgtcaa atgcgacttc gtttctgtac
    50401 cgacgcatcc gaccatcacc aaattacggg tactttcccg caaccaacag ctgatccgtc
    50461 tggatttcga agaaggtttc gaaggcgttg atccgcagcc gctgcacgag cggattaatc
    50521 aggcgctgag ttcgattggc gcgctggtgc tttctgacta cgccaaaggt gcgctggcaa
    50581 gcgtacagca gatgatccaa ctggcgcgta aagcgggcgt tccggtgctg attgatccaa
    50641 aaggtaccga ttttgagcgc taccgcggcg ctacgctgtt aacgccgaat ctctcggaat
    50701 ttgaagctgt tgtcggtaaa tgtaagaccg aagaagagat tgttgagcgc ggcatgaaac
    50761 tgattgccga ttacgaactc tcggctctgt tagtgacccg ttccgaacag ggtatgtcgc
    50821 tgctgcaacc gggtaaagcg ccgctgcata tgccaaccca ggcgcaggaa gtgtatgacg
    50881 ttaccggtgc gggcgacacg gtgattggcg tcctggcggc aacgctggca gcgggtaatt
    50941 cgctggaaga agcctgcttc tttgccaatg cggcggctgg cgtggtggtc ggcaaactgg
    51001 gaacctccac ggtttcgccg atcgagctgg aaaatgcagt gcgtgggcgc gcagataccg
    51061 gctttggcgt gatgaccgaa gaggaactga agctggccgt agcggcagcg cgtaaacgcg
    51121 gcgaaaaggt ggtaatgacc aacggtgtct ttgacatcct gcacgccggg cacgtctcct
    51181 atctggcaaa tgctcgcaaa ctgggtgacc gtctgattgt cgctgtgaac agcgatgcct
    51241 ccaccaaacg gctgaaaggg gattcccgcc ccgttaaccc gctcgaacag cgtatgattg
    51301 tgctgggcgc actggaagcg gtcgactggg tggtgtcgtt tgaagaagac acgccgcagc
    51361 gcttgatcgc cgggatcctg ccagatttac tggtgaaggg cggcgactat aaaccagaag
    51421 agatcgccgg gagtaaagaa gtctgggcca acggcggcga agtgctggtg ctcaacttcg
    51481 aagacggttg ctcgaccact aacatcatca agaagatcca acaggataaa aaaggctaac
    51541 cggaaagcgg ttcacagatc tcgcttcgta gcaggcaggc ctattgacgc tgttgttttt
    51601 tcaacctaaa gtaaaggaac aagggtaagg gaggatttct cccccctctg atcagttgtt
    51661 agtaagtcgg gaaacttaac agtaacaaca caaccagtat gatgacgaac ttcatcataa
    51721 ccctttcctt ctataaggcc ccttcctcgg gaggggcttt cccgtttcag ccccttgctg
    51781 actccccgat tgttgatgag cgccggaccc cgcgcaacgc tttccctgaa gcagttttgc
    51841 cttccccgag cggtcacgca aaacgctgca acgtcctgag catttgcacc ttttagcgga
    51901 acgcagatat aaaaaatccc cggctttttg ggccagggat atgtgttaat gaaaccttct
    51961 tgacgctgat tttttttcaa cctaaagtaa aggaacaagg gtgagggagg atttctcccc
    52021 cctctgattg gctgttaata agctgcgaaa cttacgatta acaacacaat caatatgatg
    52081 acgagcttca tcataaccct ttccttctgt aaggcccctt cctcgggagg ggctttcccg
    52141 tttcagccct ttgctgactc cccgattgtt gatgagcgcc ggacctcgcg caacgctttc
    52201 ccagaggcag ttttgccttc cccgagcggt cacgcaaaac gttgcaacga cctgatcatt
    52261 tgcagttttt agcggaacgc agatataaaa aatccccggc tttttgtgcc agggacgttc
    52321 atagctgaaa atttattcta cgttttcggc gacgggagtt gttgaggtta agggtgaagt
    52381 caatgccgcc agtgagccgc ctgaaacgcc aatttcattg agcaaggagt caatcagcgg
    52441 tgcctgtgtg cggtaagaga gagcggctga taatgcttgt tccgccaggt ttccgccccc
    52501 cacattaccc gtgtttgcat cacccgcagc gctgccacga ttcagaccat cgacctgaat
    52561 aatcttgata ccgtcaatag atttcatcgg ctcaacggat ttttctatta ccgcaggtag
    52621 cgcctgcaac aaggccagtt tgaatttaag gctggtttgt tcatcagaaa gtacgttgat
    52681 agcatcgttc agcgcacgtt gtgcttctgc ttccgccaga ccctttttac gtgtagcttc
    52741 ggctaactca acgatagccg ccgcctgcat ctctgcggct tctttttctg ctttcgcccg
    52801 cacggtcagt tcaaccgctt tggtttctgc atcctgcgcg gcagcgatta gggcaacttg
    52861 tttagcacga tcggcttctg cggtctggcg agtggtttct acgttttgtt gcgcacttac
    52921 tgcttctgcg agtgcaaggt tagcacgcgc ttctgcctgg gactgttgtt ccgatttggc
    52981 ggcaatagcg atcgatttcg tctggttggc gatttcggtg acctgctgtt gttcgatctc
    53041 tttaatacga acttcacgtt cagcctcaac ctttcttgag cggacggcct gctcgcgctc
    53101 gatctccgtt tcctgaattt gtcgttcagc cagaattcgc gtctgctctg cttcacgacg
    53161 acgttcagct tcaaaagccg caatacgtgc attctgttcg gcagtacggg ttttaacctg
    53221 ctgctcctgc tcaagcgtca taaacgcttc ttgttgttca atctccaact tgcgcgaaag
    53281 tgcatcacgg tttttctcac gcaccgcaac ttctacatcc tgttcaactt cgttacgttc
    53341 gcgacgacgg cgctccgtct cctgagtcag tttggttaaa ccttcggcgt caaaggcatt
    53401 gttcggattg aaatgttctt tcgaggtctg gttaaagttg gtaagtgaaa cgctttccag
    53461 ctcaagaccg tttttcgaca ggtcttctgc gacagtattt tgtactccct gcacgaagtt
    53521 ctcgcgggta tcctgtaact catgcatggt catttgtgca gctgttgcac ggagggcatc
    53581 gacaaattta tcttcaacca acatacgtaa gtcttctggt gatagcgtgc gttgccccaa
    53641 cgtctgcgcc gcagtggcga tgccttctac agacggtttt acccgtacaa agaaagcgac
    53701 tactacatcg acacgcatac gatctttcgt aatcaggcta tcaatggttg agcggctgac
    53761 ttccagcttc agagtattca tattgatggg gattatttca tgaaagatcg gcatcacgat
    53821 tgcgccaccg ctcatcacca ctttttgccc acctaaacca gtacgaacaa aagcttgctc
    53881 tgccgaagcg cgacgataga gcctggcgaa aataattcca ataataaaca gaatgcatac
    53941 ggcaataatc gcggtaaaca tccaggaggg cacagaatta acaatatcat ccattattaa
    54001 atttcctttt gattaataaa ttacaatatt tgtggccagg gattgttttc cgccaaatat
    54061 cgcgttgcag aaagtcggca aataattaac actttgtctc ctttagtgaa aaattttcct
    54121 tcttcaggct ccagtagtaa ataatgtatt tggccaaatt gatcggtgag ttttccttcg
    54181 cagggattac ctgaagtggc ttgatgaccg gtaattaatg ccatgctacc aatatattct
    54241 tcttctgtaa tagctgaact atggtcgcgt ggaatccagg gcgcaactat tttaccggta
    54301 taatgcaccg caataatcgt aaacagtaaa ctgacaggaa ccacgaacag gttcgacagt
    54361 ggtgattgcc agaccatgat gcaggcatgc tgcaacagga tgccaataag accaaaaaaa
    54421 ccagccagca agcagagaac gacgagagct ggcaatcttc cgatattgag ataatgaagt
    54481 gcctggctaa tatgacctgt ggttatggaa tcataatggt caagatgtgc atcaagagca
    54541 ccggagagca tgtgaccgca gataagcgct aaaatttcta aaaggccgat caacagtaca
    54601 aaggaaatag cgaaaagata aggggtatta tagtcggcga ataaaatcat atgtcact
//
LOCUS       DAJSKJ010000006        53346 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000006 DAJSKJ010000000
VERSION     DAJSKJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.006"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..153
                     /locus_tag="ON345_000304"
     CDS             <1..153
                     /locus_tag="ON345_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR3070417.1"
                     /translation="KVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGG
                     CGPTAQ"
     gene            397..1785
                     /gene="frlA"
                     /locus_tag="ON345_000305"
     CDS             397..1785
                     /gene="frlA"
                     /locus_tag="ON345_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR3070418.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENVADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIDLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIESSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1806..2828
                     /gene="frlB"
                     /locus_tag="ON345_000306"
     CDS             1806..2828
                     /gene="frlB"
                     /locus_tag="ON345_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR3070419.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2878..3708
                     /gene="frlC"
                     /locus_tag="ON345_000307"
     CDS             2878..3708
                     /gene="frlC"
                     /locus_tag="ON345_000307"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR3070420.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIIDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3705..4490
                     /gene="frlD"
                     /locus_tag="ON345_000308"
     CDS             3705..4490
                     /gene="frlD"
                     /locus_tag="ON345_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR3070421.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLDENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4590..5321
                     /gene="phoB"
                     /locus_tag="ON345_000309"
     CDS             4590..5321
                     /gene="phoB"
                     /locus_tag="ON345_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR3070422.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQGGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5473..6558)
                     /locus_tag="ON345_000310"
     CDS             complement(5473..6558)
                     /locus_tag="ON345_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR3070423.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6570..7874)
                     /locus_tag="ON345_000311"
     CDS             complement(6570..7874)
                     /locus_tag="ON345_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR3070424.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7886..8239)
                     /locus_tag="ON345_000312"
     CDS             complement(7886..8239)
                     /locus_tag="ON345_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR3070425.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8250..9128)
                     /locus_tag="ON345_000313"
     CDS             complement(8250..9128)
                     /locus_tag="ON345_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR3070426.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGFLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(9125..10351)
                     /locus_tag="ON345_000314"
     CDS             complement(9125..10351)
                     /locus_tag="ON345_000314"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3070427.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10352..11515)
                     /locus_tag="ON345_000315"
     CDS             complement(10352..11515)
                     /locus_tag="ON345_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR3070428.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHKLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            complement(11599..11961)
                     /locus_tag="ON345_000316"
     CDS             complement(11599..11961)
                     /locus_tag="ON345_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3070429.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11978..12883)
                     /locus_tag="ON345_000317"
     CDS             complement(11978..12883)
                     /locus_tag="ON345_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070430.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13152..14156)
                     /gene="trpS"
                     /locus_tag="ON345_000318"
     CDS             complement(13152..14156)
                     /gene="trpS"
                     /locus_tag="ON345_000318"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR3070431.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14149..14907)
                     /gene="gph"
                     /locus_tag="ON345_000319"
     CDS             complement(14149..14907)
                     /gene="gph"
                     /locus_tag="ON345_000319"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR3070432.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14900..15577)
                     /gene="rpe"
                     /locus_tag="ON345_000320"
     CDS             complement(14900..15577)
                     /gene="rpe"
                     /locus_tag="ON345_000320"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3070433.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15595..16431)
                     /gene="dam"
                     /locus_tag="ON345_000321"
     CDS             complement(15595..16431)
                     /gene="dam"
                     /locus_tag="ON345_000321"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR3070434.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16538..17824)
                     /gene="damX"
                     /locus_tag="ON345_000322"
     CDS             complement(16538..17824)
                     /gene="damX"
                     /locus_tag="ON345_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR3070435.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17916..19004)
                     /gene="aroB"
                     /locus_tag="ON345_000323"
     CDS             complement(17916..19004)
                     /gene="aroB"
                     /locus_tag="ON345_000323"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR3070436.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19061..19582)
                     /gene="aroK"
                     /locus_tag="ON345_000324"
     CDS             complement(19061..19582)
                     /gene="aroK"
                     /locus_tag="ON345_000324"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR3070437.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19983..21221)
                     /gene="hofQ"
                     /locus_tag="ON345_000325"
     CDS             complement(19983..21221)
                     /gene="hofQ"
                     /locus_tag="ON345_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR3070438.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21133..21537)
                     /gene="hofP"
                     /locus_tag="ON345_000326"
     CDS             complement(21133..21537)
                     /gene="hofP"
                     /locus_tag="ON345_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR3070439.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21527..21967)
                     /gene="hofO"
                     /locus_tag="ON345_000327"
     CDS             complement(21527..21967)
                     /gene="hofO"
                     /locus_tag="ON345_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR3070440.1"
                     /translation="MNMFFDWWFAISPRLRQFCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21951..22490)
                     /gene="hofN"
                     /locus_tag="ON345_000328"
     CDS             complement(21951..22490)
                     /gene="hofN"
                     /locus_tag="ON345_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR3070441.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22490..23269)
                     /gene="hofM"
                     /locus_tag="ON345_000329"
     CDS             complement(22490..23269)
                     /gene="hofM"
                     /locus_tag="ON345_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR3070442.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLVWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23389..25941
                     /gene="mrcA"
                     /locus_tag="ON345_000330"
     CDS             23389..25941
                     /gene="mrcA"
                     /locus_tag="ON345_000330"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR3070443.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26106..26666)
                     /gene="nudE"
                     /locus_tag="ON345_000331"
     CDS             complement(26106..26666)
                     /gene="nudE"
                     /locus_tag="ON345_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR3070444.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMILPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            26986..29121
                     /gene="igaA"
                     /locus_tag="ON345_000332"
     CDS             26986..29121
                     /gene="igaA"
                     /locus_tag="ON345_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR3070445.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29186..29854
                     /gene="yrfG"
                     /locus_tag="ON345_000333"
     CDS             29186..29854
                     /gene="yrfG"
                     /locus_tag="ON345_000333"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR3070446.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDIYAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29865..30266
                     /gene="hslR"
                     /locus_tag="ON345_000334"
     CDS             29865..30266
                     /gene="hslR"
                     /locus_tag="ON345_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR3070447.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRTLAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30291..31169
                     /gene="hslO"
                     /locus_tag="ON345_000335"
     CDS             30291..31169
                     /gene="hslO"
                     /locus_tag="ON345_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR3070448.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31304..33028)
                     /locus_tag="ON345_000336"
     CDS             complement(31304..33028)
                     /locus_tag="ON345_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR3070449.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33407..35029
                     /gene="pckA"
                     /locus_tag="ON345_000337"
     CDS             33407..35029
                     /gene="pckA"
                     /locus_tag="ON345_000337"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR3070450.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPAQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35105..36457)
                     /gene="envZ"
                     /locus_tag="ON345_000338"
     CDS             complement(35105..36457)
                     /gene="envZ"
                     /locus_tag="ON345_000338"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR3070451.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36454..37173)
                     /gene="ompR"
                     /locus_tag="ON345_000339"
     CDS             complement(36454..37173)
                     /gene="ompR"
                     /locus_tag="ON345_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR3070452.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37401..37877
                     /gene="greB"
                     /locus_tag="ON345_000340"
     CDS             37401..37877
                     /gene="greB"
                     /locus_tag="ON345_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR3070453.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37974..40295
                     /locus_tag="ON345_000341"
     CDS             37974..40295
                     /locus_tag="ON345_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR3070454.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQSAGNSAMMDALAAAMGKKR"
     gene            40733..40960
                     /gene="feoA"
                     /locus_tag="ON345_000342"
     CDS             40733..40960
                     /gene="feoA"
                     /locus_tag="ON345_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR3070455.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40977..43298
                     /gene="feoB"
                     /locus_tag="ON345_000343"
     CDS             40977..43298
                     /gene="feoB"
                     /locus_tag="ON345_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR3070456.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43298..43534
                     /gene="feoC"
                     /locus_tag="ON345_000344"
     CDS             43298..43534
                     /gene="feoC"
                     /locus_tag="ON345_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR3070457.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43737..44615
                     /gene="rpnA"
                     /locus_tag="ON345_000345"
     CDS             43737..44615
                     /gene="rpnA"
                     /locus_tag="ON345_000345"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR3070458.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44644..45414)
                     /gene="bioH"
                     /locus_tag="ON345_000346"
     CDS             complement(44644..45414)
                     /gene="bioH"
                     /locus_tag="ON345_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR3070459.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45452..46135
                     /gene="gntX"
                     /locus_tag="ON345_000347"
     CDS             45452..46135
                     /gene="gntX"
                     /locus_tag="ON345_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR3070460.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRYWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46194..46769
                     /gene="nfuA"
                     /locus_tag="ON345_000348"
     CDS             46194..46769
                     /gene="nfuA"
                     /locus_tag="ON345_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR3070461.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47130..48446
                     /gene="gntT"
                     /locus_tag="ON345_000349"
     CDS             47130..48446
                     /gene="gntT"
                     /locus_tag="ON345_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR3070462.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48557..50641)
                     /gene="malQ"
                     /locus_tag="ON345_000350"
     CDS             complement(48557..50641)
                     /gene="malQ"
                     /locus_tag="ON345_000350"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR3070463.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50651..53044)
                     /gene="malP"
                     /locus_tag="ON345_000351"
     CDS             complement(50651..53044)
                     /gene="malP"
                     /locus_tag="ON345_000351"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR3070464.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        12614 a        14674 c        13523 g        12535 t
ORIGIN      
        1 aaagttgcac tggtaggtgc agtactggct acgttaactg catgtactgg tcatattgaa
       61 aaccgtgata agaactgctc ttacgactac ctgctgcacc cggcaatttc tatttctaaa
      121 atcattggcg gttgcggtcc tactgcacag taagtctctg acagaaatcg gctaacaccg
      181 gtttaattgc ccgacgccct ctggattttc cgagggcgta tttgtttcta tgtccttctt
      241 gcccccgccc gtaaaaatat tttcacttta aattcaattt gataactaca tccattagcc
      301 acagcgcgat cctgtgcgaa attttgtgat cttcctccac attacataac atcatatgtt
      361 gttatattca tcatgcattg tcatgttacc ttttaaatga ctgcaaactc tcccctacaa
      421 cgtattggac aagaaaaagg tatcgctatg ggaagccagg aactccaacg caagctcgga
      481 ttttgggccg ttcttgcaat cgccgtcggg acaaccgtcg gctccggtat ttttgtatct
      541 gtgggtgaag tggcaaaagc agcgggcacg ccgtggctta cggtgctcgc gtttgtcatt
      601 ggcgggttaa ttgtgatccc gcaaatgtgc gtctatgcgg aactatccac cgcttatccg
      661 gaaaatgtcg cagattatgt ttatctgaaa aatgccggaa gccgaccgct ggctttcctc
      721 tccggctggg ccagcttctg ggccaacgat gcgccgtcat tgtcgattat ggcgctggcg
      781 attgtcagca atcttggctt tttaacgcct atcgatccgt tgctcggtaa atttatcgcc
      841 gccggattaa ttatcgcctt tatgttgcta cacctgcgct ccgttgaagg cggcgcagcg
      901 tttcagacgc taattaccat cgccaaaatt atcccgttca ctatcgtcat tgaccttggg
      961 atcttctggt ttaaagcgga gaattttgcc gcccctacca ccactgcgat tggcgcaacg
     1021 ggcagcttta tggcgctgct ggcggggatc tctgccacca gttggtcgta taccggcatg
     1081 gcctctatct gttatatgac cggcgaaatt aaaaaccccg gaaaaaccat gccacgagcg
     1141 ctgattggtt cctgtctgct ggttctggtg ctctacaccc tgctggcgct ggtgatttcc
     1201 ggcctgatgc ccttcgacaa actcgccaat tctgaaacgc cgatttccga cgccctgacc
     1261 tggatccccg cactcggcag caccgctggg atctttgttg ccatcacggc gatgatcgtc
     1321 attcttggtt cgctttccag ctgcgtgatg taccagccgc ggctggaata cgcgatggcg
     1381 aaagacaacc tgttctttaa atgcttcggc catgtgcatc cgaaatacaa cacgccggat
     1441 gtctccatca tcctgcaagg ggcgctgggg atcttcttca tcttcgtttc cgatctcacc
     1501 agcctgctgg gttatttcac cctggtgatg tgtttcaaaa ataccctcac cttcggctcc
     1561 atcatctggt gtcgtaaacg cgacgattac aaaccgctgt ggcgtactcc ggctttcggg
     1621 ctgatgacca ccctcgccat tgagtcaagc ctcattctgg tcgcctcaac ctttgtctgg
     1681 gcaccgattc ccggccttat ctgcgccgtc atcgttattg ctactggtct gcctgcttac
     1741 gccttctggg cgaagcgtag ccgccagctc aacgctttgt cgtaatgtta tctggagaaa
     1801 ataaaatgtt ggatattgat aaaagcaccg tggactttct ggtcaccgaa aatatggttc
     1861 aggaagtgga aaaagttctc agccatgacg ttccgctggt gcacgccatc gtggaagaga
     1921 tggtgaagcg cgacattgat cgtatttatt tcgttgcctg cggctcgcca ctcaacgcgg
     1981 cgcaaacggc gaaacatctg gcggatcgct tttccgatct tcaggtctac gccatttccg
     2041 gctgggagtt ctgcgataac accccgtatc gcctcgacga tcgttgcgcg gtgattggcg
     2101 tttctgacta cggtaaaacg gaagaggtaa tcaaagcgct ggagctgggc cgggcctgcg
     2161 gcgcactcac tgcggcgttc accaaacgcg cggacagccc gattacctcg gcggcggaat
     2221 ttagcattga ttatcaggcc gactgtatct gggaaattca cctgctgctc tgctacagcg
     2281 tggtgctgga gatgatcacc cgcctcgcgc cgaacgcgga aatcggcaag atcaaaaacg
     2341 atctcaagca gttgccgaat gcgctcggtc atctggtacg cacctgggaa gaaaaaggcc
     2401 gccagcttgg tgaactggcc agccagtggc cgatgattta taccgttgct gcgggtccgc
     2461 tgcgtccgct gggttacaaa gaaggcattg taacgctgat ggaatttacc tggacgcacg
     2521 gctgcgtgat tgagagcgga gagttccgcc atggcccgct ggagattgtc gaaccgggcg
     2581 ttccgttcct gttcctgctc ggcaatgatg aaagtcgcca caccaccgaa cgcgccatta
     2641 actttgttaa acagcgtact gacaacgtga tcgtcatcga ttacgccgaa atttcgcaag
     2701 ggctgcaccc gtggctggca ccgttcctga tgttcgtgcc aatggagtgg ctctgctact
     2761 acctgtctat ttacaaagat cacaacccgg atgaacgccg ctattacggt ggtctggtgg
     2821 aatattaatc cctctcccgg cccggcaacg ggccggattt acgcaaggag ttacccgatg
     2881 aaaacaggta tgtttacctg cggccaccag cggctgccta ttgaacacgc atttcgtgat
     2941 gcaagcgagc tgggttacga cggcatcgaa atttggggcg gtcgcccgca cgcgttcgcg
     3001 ccggacttaa aagcgggcgg catcaaacaa atcaaggctc tggcgcagac gtatcagatg
     3061 ccgattatcg gctatacgcc agaaaccaac ggctatccgt ataacatgat gctgggcgat
     3121 gaacatatgc gtcgcgaaag cctcgacatg atcaagctgg cgatggatat ggcaaaagag
     3181 atgaacgcgg gttatacgct gatttccgcg gcccacgcgg gctatctcac gccacctaat
     3241 gttatctggg gacggctggc agagaaccta agcgaactgt gtgagtacgc ggaaaacatc
     3301 ggcatggatc taatcctcga accgttaacg ccgtatgaat cgaacgtcgt gtgtaatgct
     3361 aatgatgtgc ttcatgcgct ggcgctggtg ccttcgccgc gcctgttcag catggtcgac
     3421 atttgcgcgc cgtatgtcca ggcggaaccg gtgatgagtt atttcgacaa actgggcgat
     3481 aaattacgtc atctgcatat tatcgacagc gacggggcca gcgacacgca ttacattcct
     3541 ggagaaggca aaatgccgct gagggaactg atgcgcgata ttatcgaccg gggctatgag
     3601 ggttactgta cggtggagct ggtgacgatg tatatgaacg agcccagact ctatgcccgc
     3661 caggcgctgg aacgctttcg cgcgctgctg ccggaggatg agagatgaaa accctggcga
     3721 caatcggcga taactgcgtc gatatctacc cgcaactgaa taaagcgttt tctggcggta
     3781 atgcggtcaa tgtggcggtg tactgcactc gctacggcat acagccggga tgcattacct
     3841 gggtgggtga cgatgactac ggcacaaagc tgaagcagga tctcgcccgc atgggcgtcg
     3901 atatcagcca tgtccatacg aaacacggcg ttaccgcaca aactcaggtg gaactgcacg
     3961 acaatgatcg cgtttttggc gactacaccg aaggcgtgat ggccgacttt gccctgagtg
     4021 aagaggatta cgcctggctg gcgcagtatg acattgtgca cgcggcaatc tggggacatg
     4081 cggaagacgc attcccacag ctgcacgctg cgggcaaact taccgctttc gacttctccg
     4141 acaagtggga cagcccgctc tggcagacac tggtgccgca tctcgatttt gcctttgcct
     4201 ccgcaccgca agaagacgaa gcgctgcgtc tgaagatgaa agcgattgtt gcccgtggcg
     4261 caggcgtggt gattgtcacg ctggatgaaa acggcagcat tgcctgggat ggcgcgcagt
     4321 tctggcgtca ggctcctgaa ccggtgacgg ttatcgacac catgggtgcc ggagattcgt
     4381 tcattgccgg attcctttgc ggctggtctg cggggatgac attaccgcag gcgatggcgc
     4441 agggaacggc gtgcgcggcg aaaaccattc agtaccacgg tgcctggtag gtataacgtt
     4501 ggcgtgagca tcttcacgcc aacgtgctgt tacttgccgg aaaacgaccc tataatccga
     4561 gtaattcatt ctttatttca gggtcgatta tgtcagctac ggaccgctac tctcatcaac
     4621 tcctctacgc taccgtccgc cagcggttgc tggatgatat cgcgcagggg gtttaccagg
     4681 gcgggcaaca gatccctacc gaaaacgagc tttgtacgca atataacgtc agccgcatta
     4741 ctattcgcaa agctatcagc gacttagtgg cagacggcgt actgatccgc tggcagggca
     4801 aaggcacctt tgtacaaagc cagaaggtcg aaaacgccct gcttactgtc agcggtttta
     4861 ccgattttgg cgtctcacaa ggcaaggcga cgaaagagaa agtgatcgag caggaacgga
     4921 tcagcgccgc gccgttttgc gaaaagctga atatccccgg taacagcgaa gtgttccatc
     4981 tctgccgggt gatgtatctc gataaagagc cgctgtttat tgatagttcc tggatcccgc
     5041 tgtcgcgtta tcctgacttt gatgagattt acgttgaagg aagctccacc tatcagttat
     5101 ttcaggagcg tttcgacacg cgagtggtca gcgacaaaaa gaccatcgat atctttgccg
     5161 ccacccgccc gcaggcaaaa tggctgaaat gcgaactggg cgaaccgttg tttcgcatca
     5221 gcaaaatcgc ctttgatcag aatgacaaac cggtgcacgt ctccgaactc ttctgccgcg
     5281 ccaatcgcat caccttaacg attgataata aaagacatta accgtaggcc ggataagatg
     5341 cgccagcatc gcatccggcg atgctggcgc gttgaatttt acatcccgta cgttcccctc
     5401 accctaaccc tctccccaaa ggggcgaggg gaccgttcag tacggaattt agcttgacgg
     5461 tgctatccag cattaaatac tggcaatact ctcacgcaaa atccgcagca ccgtctcttc
     5521 accgctgcga ttcgggttaa tgcgaatcgc acaatgttct gattgtggat tcgcctggcg
     5581 aaacgttccg gaaaggcgat aaaagagcgg cgggatttca tatttcgact ctgcacccac
     5641 cgggtaaggc aaggcaccgc gcttttgcgc ctcttccagc actctggcgg caatcggctg
     5701 atgaaactcg acaatcaaca ccttcgactg cgcattagca atcaccgcgc ttttcacttc
     5761 cggcaccgca ccaccgttaa gcaaagccag caaccgttca gatacccctg cctgcaccgc
     5821 gtgcatcact ggcgcaaaca ccagaccacg caatacttcc agcgcctgtg cgccctggat
     5881 ctggctaccg ccggagtaaa gcgtggcgcg aatacggttg ataacatcag catcgccgac
     5941 cactgcacca acaccctctg gcccaaatag cttgaagcag gaaaatgtcg agacattcgc
     6001 gccgcattca cagccgattc gcgccacctt catcaccgca tagttgtcat cggttaacgc
     6061 tggaacacct gccgcgcgca acgttgccag cacatctgcc agcacgtagc tgtcctgcgg
     6121 ctgctggcgc gtatgctgca ccagcgccgc atccggttgt tgctcgtcga cgacctgctt
     6181 cagtgccgac aggtcattga aatcaacagt aataagcgtc agccccatct gctcaataat
     6241 aacccgtgtc gtcgggtaaa caggcgcgtc atgcaccaga agacgctgcc ccggtttgag
     6301 caaagccgcc agcccggcgc gaatcgcgcc agtccccgcg ccctgcacca gcgccgcagc
     6361 ctgtgcgtga aatgcatcag ccagcacctg ttccacacgc tgggtaacgc gcggttgatt
     6421 cagtcctggc gttaagccta aatcaccgcc agtaagaaac tcgctgccgg ggaaatggcg
     6481 acaaatgcta tccaccagcg caaactgctt ttgctgcgcc tcaataatcg tcaggctttg
     6541 cagaggaaac gtcttcatag gtgccttcct taagccggaa cgaacaggcc aagccagtaa
     6601 agaacattca gcagaatacc ggtgatcatt actgccacga ccggtgccgc cattttctgt
     6661 accggacggc ccagggattc gttgaggaag taaatggcaa ccgcgataga gaatccggta
     6721 taacccgcca tcttaattgc tgcgaaaatc gaaccgacca gcagcgccac ttccatcagc
     6781 atattcatgg cgttacggat gttatccgac gcattacgca ccgacgggta gcgtcccagc
     6841 catttgccga tcgaacgaag cagcaagact tccgccgaaa taaccactgc gcctaatacc
     6901 gctgcaacca tcggattcgg cgagagatag cccaccgcat aaacaaaggt aaagcccgca
     6961 actgcataca cacccgttgc taaggcggtg gtggcaatca acggtacaaa acccagcccg
     7021 cgcataaatt ctgccagtgc cgcctggttg atcagcgttt gcgattgttc cggcgttacg
     7081 cctgcggagt acgctttctc cagagtgaag atcgacactt cactgccggc gaaaatcttc
     7141 atgctggcaa cggcggcaat caatgctccc acgatggcga tatagggtaa gtttttgata
     7201 atccgcgacg tgcgttcttc aaacaccgaa agcccgctgg catcaatatc attttcatca
     7261 cgatgacgca ggtcgtgagt tatcgcgatc cccagcagca tcaccatgcc aataaagatt
     7321 tcgatggatt cagggttaag atgtgggaaa tagcgcacga ctaccacacg ggtcatcaga
     7381 acaaccacgg cggcgaccag actttgcttc cagccaaact ggtagaaaat cgccaccagc
     7441 gggaacagtg caaaagcaga aaccaccggc gagcttaatt ccccgaggct acctaataca
     7501 tccaccggca gcgcggtcag cagctggttt actggcaaca ggcaagtaag gatcaatacg
     7561 ccccagatag cgccaagacc aaacgccatc aggctgttta tcgccagtac gccgaggata
     7621 tcggtaggaa gaaagagtaa ccatgcgttg agcagtccgg ttttcagggt aaaagagatc
     7681 cccaccgagg ccacaaaacc aatgctcaga ccaaaagcga tactccccgc ctcgcgacgg
     7741 ttcatatagc cttcaatcag ttgcggcagg atcgggcgga tgccgtcatg aaaaacagcc
     7801 gccgagcgat gcgccagaag cgatgtcata cccgtcaggc acgccaccac gataatctga
     7861 atatacagat ccatagtcgt gccttttatt tcagatggtt aatcagcatc ggaatcgcgt
     7921 gttcgacgtg ctcaacggaa aggccaaacg ccacttttcc ttcagcgatc attttggcga
     7981 tatgctcgtc tttcgcttta atgcctggtt tggcaatggt gcaacttttg ttatagccaa
     8041 tcaccgcgat ggcaatcgac aatgccgcgc ccgcaccggt attacacgca ccgatgtagt
     8101 aatccagttg cccggatttc actttcattg ccgcttccat gtcgttgtga atgaaaactt
     8161 caaaactgcc aggagccgtc gcttcaatgg tttttttaat ctgctcacgc tgtaagcctg
     8221 caacgccaat ctttttcatg agtctgtcct tattggaaaa attgagaggg agtttcacgt
     8281 aacatcacat ccacatcggc ctgactgaat cctgactggc gcaattgcgg aataaaggtg
     8341 gtcaataaat agtcatagcc ataaccacca ttggctttta aatgggagcg gcgcgtaata
     8401 tccatcgaca gcatgacgcg gttcagaaac ccacggtcac gtagcgcgtg gagcatcgca
     8461 atgcgctttt cgtccgggta gtaactgttc ttaccaatgg tgtcgaactg cacgtacgcg
     8521 ccgagatcga tcatcttcaa aatgttgtcg aggttgtctt tcagatcgca gtgaccaacg
     8581 gtgacgcgcg aaagatcaac tccgtgggct tgcaacagag ccagttgttc cagccccatg
     8641 gtgctgaacg acgtatgcgt ggagatcggg cgtccggtct ggttatgcgc cagcgcagcg
     8701 gcaataaata ccttctcttc cagcggcgta atctttcctt cgctggagcc gatctccgcg
     8761 atgatcccgg ctttcagctc cgtgccatcg ataccctgtt caatttcatc gaccatctcc
     8821 tgcgccagtt cctgcacgct gcgggtcacc acatgttccg ggaaaaacgc atcctggtaa
     8881 taaccagtac aggccaccac gttgatcccg gtttcgcgca ttacatcgag cataaattgc
     8941 gcattgcgcc ccatgtaacg gttggtcatc tcaatcacat tacgtacacc ccgggtcatc
     9001 aggtcgttca tctcctggca aatgaacgca tactgatcaa ggcggcagtc cacgttgttt
     9061 ttaaagccgg agagatcaat atgcagatgc tcatgggcca gggtgtaacc cgtcggatca
     9121 aaactcatag ggtgtctcct gcaaaccgga gggaggaaag aaaagagcga ccattttgtg
     9181 gcggtggcgc acggaaaaat tcacacacgg tagctcccac atcagaaagc gtggtgcgca
     9241 caccgagctg cgtggcgacc agcccttgct gataaaccag cactggcacc acttcacggg
     9301 tatggtggct gtgaccaatg gtcggatcgt taccgtgatc cgccatcacg accaggcaat
     9361 catctggctg catcgcctca acaagccggg caaggttacg gtcaacgacc tgcaaacgtt
     9421 cggcataacg tgcgacgtct tctgcatgac cagcgaggtc ggtttcctgg atattggtgc
     9481 aaataaacgc cgtcggatga gtgttaaatt cgtcgagggt gatatccata atccgctggc
     9541 tatccaccag gttttgccag ctcacgccat aaggattgct gacgatatct gccaccttac
     9601 ccaccagcac ggttggcacg cccgcctcat acagtttttg cggcacctgc acttttttat
     9661 cgacaccgta gcccatatgc acgacctgga agccgttgtc ataagcgcca gaacgcggcg
     9721 cattgatacc aataaagcgc ccttctttgc tttctgcggc atcgagaatg cgttgactgt
     9781 cggttaacag gccaccaaat gtaatgaccc gaccgacctg tacctgctca cgcacgatac
     9841 gaccaatttt gattgcgtcg tcaaaagaga tcacagagag attggcggta atgttataga
     9901 cctggcctaa atccgcctcg agattatcgc caatcgcaac cgcctgattg acccacagaa
     9961 attgcagttc atcgccacgg cgctccacct gccaaccggc ggatactaat gcctgctcaa
    10021 cacgatcaat cacatcgcga aagggcatcc gcagcggcgg taacgggcgc gtgcctaaaa
    10081 tttcctgatg ccccataaag gtatcgccac cttcatgttg cagctctgcc acgccccagg
    10141 ttgcggagtc tgacggctgc atatcgcctg gcgcataacc caatgcgttg attagcccca
    10201 gcttctccag cgttggtagc tgcaaatgcg gcaactggct caggatgtga ccacaggtat
    10261 tcgccccggc atcctgcgga cgcaccagcg tgacatcttt cattgcgcct acgccaaagc
    10321 tatcaatcac taacaccaca aatcgcgcca tttacgcctc cagagggttg ccgagactgt
    10381 cataacgacc aacgattttc ggttcgccac ggtgaatacc ggacaccagc accacatcgc
    10441 tacgggtgac aaaaatctgc gtgcggaaac agagcaccac tgcactgctc accggatact
    10501 cgcctgccag cggcagggtg tagtcgatac tgctgtcatc cacagttttg agattggttt
    10561 cagtaatctt ttgattttct ggcgtaaaca ccagcgcatg ttgcgcatga ccacgacggt
    10621 agtaaccgcc gccgtagcag tagctgtcgc cacggaaatg atgggagatt tcacttaacc
    10681 agagcatcgc gatacgttca ggctgatcgc cctgctggtt tgccggaata gtgcccgtca
    10741 gcgcatgacc gggttcggca tgagtcacac cgtattccgc cagcaatggc agcgaagtgc
    10801 agctggtcgc tgaaggcgcg ttcagttgct caagtgcaat accagatttc gccagttgat
    10861 cccgtgcctg tatcagctta tgaagattcg gtgtcggcaa aacttttccg gcagcctcat
    10921 cccaaagcag gcaagggaaa tgggtaagtc cggctaaatg cagccccggc agtttcttaa
    10981 tttcagcaac cacctcgtgc agcgaatgct gtacaaaacc gctctcctgc cccggataaa
    11041 gaaaatcatc gtcgctataa actttgagca gcacaaactg cacacgtccg gccttcaccg
    11101 ctgccgcaga aacttcccgc gctttgtcga gagtaaacac ggtgatgacg tcggtgccct
    11161 gttcaacggc gtcagcaacc tgatgacaag ggatttgtac cagatgcccc tgatgcgcca
    11221 ccgacaaacc agcgcggcgc atgactcgcg cctctttgta atccaccgcc acaatgccgc
    11281 tgtaacctaa tgccagcaat ttttccgcca gccatgggtt acggccaaac tgcttggtca
    11341 tcagatacag ttcaatgccg taaagccgcg ccgtctcaat cagtcgctta ccgttttcca
    11401 gtacctgatc cacgtcgatc acccagctgt ccggggcgat cttgccctgc tgccacaaac
    11461 ttagtgcggc ggaaatcagc gccgggttct ggcgtttcaa tgcttctaca aacatcttgt
    11521 tgcctcgttt ctgttccttg aattaaatat tcattttttt gaatatttag gttgtgcgac
    11581 gtggatttgt cgggagcttc aaacctcttc gttagcagcc atccataatc cataaaggtt
    11641 ggccagcaaa tagccttctt cacacgggtt aacttccagt gcgaattcct tcaacaacac
    11701 ctgatgcaat tgcacaacgg cttgccagtg gctggattgc gccagctctg ccagcaattc
    11761 gttatccagc ggctctattt cttcaccacg gcgactgcgc atcagtgcac tcgccatatg
    11821 tgtcatcgcc atcgttcctt gctcactgcg caccggcaga tggcactctg tttccagtac
    11881 gttgacgacc tgcatcatgc ctttgcagat gtccttatca ataacgcctg cctcgcaaag
    11941 caggttgagt ctggtttcca tatcagatgc ctttcgttta ataactcggt tcctgttccc
    12001 cactcactac ccgttgttga tgggcgagca gggcgtgttt atctacaacg gcacgcagca
    12061 gttgttgcat tgggtaatca tcgactcgcg tcaggaccac ggcttcggtg gcctgtaaaa
    12121 atcgcggatc gtctgtgagc ggcgtcgcct ctaatcccag catggtcagt tcgttttccg
    12181 ccaccacgtt ccagatcacc gcatcgacat cgcctttaac aatgcgttgt aaactctcgt
    12241 gataagagag atcgactcgt tccacatcac taccgccaaa aaaaacatcg gtcatgattt
    12301 tctgatccgc cgaacggtta tccagcccca cgcgcttcac gtttgcggac tcgcctttac
    12361 ggcaaatcaa ctggtgctcg ccaacgtagg tgtgcggccc caactccagc gcgaggcata
    12421 accctttttg cgtgagataa ctttccgccg ccagtcggga aaccaccgcc atgtcataca
    12481 cgccgttaag taaacactcc acgcgaatat ccgcgccgcg catatgcgca tagtaaaaag
    12541 gaatgccatc aaactgagct ttcagcccgc tcgctaaacc ttcgtacaag cgggtatagg
    12601 gcaaaggcat cgcacatacc acgttgttga tatccacatg actcagcaat gctttgttat
    12661 ccatctcgac cagataactg ccattgcgcc cacggcgttc aatccgtatc gctccgcttg
    12721 attccagcgt tttcaacgcg gcctgcgtca ggccaaccga tgaacggcat tcagttgcca
    12781 gctcatctat ggttttcaac ctattaccgc atttttcacc caacaaataa cgggccagcg
    12841 tcgtgataac gacgccttct tttttgataa acgttcgacg cataatatat tttcaataaa
    12901 atgaatgttt atatcttcat taaagtgaag ataagtggca actgtgagat gtggaaacgg
    12961 cgaggcgctt cacatttttt cggattattg caaatgagga taaaaaaacc gggtttcccc
    13021 ggtttcagag tgatgacgaa gtgatttatt cgacaatgcg agcaaagggt aattcccact
    13081 ggcgcttgcc tgctcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccga
    13141 gcgttttgat attacggctt cgccacaaaa ccaatcgctt cgtacaccgc tttcagcgta
    13201 cgggaagcgt gtacactggc tttttctgcg ccgtctttca tcacctgttg caggaaggct
    13261 tcatcgttgc ggaaacggtg atagcgttcc tgcaattcag tcagcatacc ggaaacggca
    13321 tcagccactt cacctttcag atgaccatac atcttgcctt cgaactgttt ttccagttct
    13381 gggatgctct ggcccgttac cgcagaaagg atatccagca ggttggaaac gcccgctttg
    13441 ttctgcacat cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc gcgtttgatt
    13501 ttcttcacta ccgatttcgg atcttccagc aggccgataa cgttattgcg gttatcgtca
    13561 gacttggaca tcttcttggt cggctccagc agcgacatta cgcgcgcgcc agatttcgga
    13621 ataaacggct ccggcacctt aaagatctcg ccatacagcg cgttgaaacg ctgggcgata
    13681 tcgcgactca gttccaggtg ctgtttctgg tcttcaccca ccggtaccag attagtttga
    13741 tacagcagga tgtccgcagc catcagcacc ggatagtcaa acagaccagc gttgatgttc
    13801 tcggcataac gcgcagattt atctttaaac tgggtcatgc ggctcagttc gccgaagtag
    13861 gtatagcagt tcagtgccca gcctaactgc gcatgttctg gtacgtggga ctgaacaaaa
    13921 atggtgcttt tctcaggatc gataccacaa gccagataca aggccagcgt atccagcgtc
    13981 gctttacgca gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc aacgatacag
    14041 taaatgcaat ggtagtcatc ctgcatgttt acccactgac gcagcgcacc catgtagtta
    14101 ccaatggtca attcacctga gggctgtgcg ccactaaaaa cgatgggctt agtcattttt
    14161 cgattcctga ttttcgctat gcggaagccc taatgcgggc agaaggtcat ttatagactg
    14221 ataaattaca tcaggctggc tgagatcgat agcctcgccg tagttatatc cgtaggttaa
    14281 gccaactgat gggcaacctg ccgcttttgc cgcctgaata tcattgcgtg agtcgccgac
    14341 aaacagcatc tcttgtgggg caattcccat ccgctcagcc accagtaaca gcgggtccgg
    14401 atgcggtttt ttgttttgca catcatcgcc gccgataacc acgctaaagt atttggcaat
    14461 atctaaggct tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga ccaggcctag
    14521 cggcaggcct ttagcctgca acgcgcccaa cgtatcggca acgtgcggga acaaaaacgt
    14581 cccctcttcg gcaacctcgc tatagtagcg atcgaacagt ttacgcagaa tacgtacctg
    14641 ttcttctgcc ggaatgtcgt catcaacggg cggtttaccc attgttttac gcagagtcgc
    14701 acgttcctga cgcgcccagg ccaatgcacg ctccatcaga acatctgcgc cgttaccaat
    14761 ccaggtaata acgcgttctt cacctgcgac gggtaactcc agcgcataca gcgccatatc
    14821 taccgcagca gcaagaccgg gagcactatc aaccagcgta ccgtcgagat caaaagcgac
    14881 gccgcgaata tcttcaaact tattcatgac ttacctttgc cagttcactg cgcatttcat
    14941 caatgacttt tttgtagtct ggctggtcga agattgccga accggcgacg aacatatccg
    15001 cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac gccaccgtcc acttccagtc
    15061 gaatgtcaaa gccagactcg tcgatacgac gacgtacttc gcgcagttta tccagtgttt
    15121 gaggaatgaa agactgaccg ccgaaaccag ggttgacgga catcagcagg atcacatcca
    15181 gcttatccat cacgtaatcc agatagctca gaggtgtcgc cgggttaaat accagacccg
    15241 ctttacagcc attttcttta atcagttgca gcgtgcggtc aacatgctcg gaggcttctg
    15301 gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg cgatcgacag
    15361 gtttcaccat caggtgtacg tcgatagggg cggtaatgcc gtagttacgc aaggatttca
    15421 gcaccattgg cccaatcgtc agattgggaa catagtggtt atccatgacg tcaaaatgca
    15481 cgacatcagc gccagctgcc agggcttttg cggtatcttc acccaggcgg gcaaaatcag
    15541 ccgacagaat tgagggggca atcaaatact gtttcatccg cttctccttg agaattattt
    15601 tttcgcgggt gaaacgactc ctggtttgta caaagccagc agttcgtcca cctttttacg
    15661 tgtgccgccg ttgctgctta tactgcgtcg aactttgacg acatgcaatt ttgcgcgctg
    15721 ataccactca cgcgttaaca tcgtatcgtg attggagatc agcactggaa tatggcgctc
    15781 aaccagacct tcggcgatct ccgccagatg cgcttgttgt tcaagcgtaa aactgtttgt
    15841 gtgatacgcc gtaaagttgg cggtcgcaga cagcggtgca taaggcggat cgcaatagac
    15901 gacggatgca tcatctgcgc gcgccatgct atcggcgtaa gactcacaat agaaaaaggc
    15961 attctgcgct ttttcagcga agtgatacaa ctctgcttcc gggaaatagg gttttttgta
    16021 gcggccaaac ggcacgttaa actcaccgcg cagattgtaa cgacacaggc cgttgtaacc
    16081 gtagcggttc aaatataaaa acagtaccgc ccgacggaac ggatcctggc ttttgttgaa
    16141 ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt tcgggaacaa acagctcgcg
    16201 tgcggcctgt acgtactcat cagtacgcat cttcacaatg ttatagagac tgatcaggtc
    16261 gctattgata tcggcaagga tataacgaga aaagtcggtg ttgagaaaca ccgacccggc
    16321 acctacaaaa ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa tatcatcaag
    16381 caggggatac ttgccccctg cccacttcaa aaaagcgcga tttttcttca tgctgactaa
    16441 ctaattacac cttctccggc tgtggagaaa gctccgacag catccagcgc ttcaggcagc
    16501 tcccgcaacg ataaaccatt gcgggagata accttgatta cttcagatcg gcctgtacct
    16561 gacgcagcgg tttcgcccac gggtttttgg cctgaacatc tgctggcaat gtagataccg
    16621 cttttttcgc ctcttctttc gaagcgtaca cgccagaaac caggacatac cacggctgac
    16681 cattacgcgt cgtttcatag acaacgtagt ttttcagatt ctctttcttc gcccaaccgt
    16741 tcaggttgtc gtagttagag gaactgctca gctgcagagt gtaatggctg gacggagccg
    16801 atttcaacga accaacatta cctgcggtct tccctccagc ggctggtgtt gccgtggttt
    16861 gcgccgggga tgccgtctgt actggagccg tagtcgccgt ctcttttggt gctggcgtag
    16921 aagtcgcagc cggtgccttc gtgctcgcca caggggcagc tggttcagta cgcttcggcg
    16981 tctgtgctac cggcttcggc tccgttttca cggttgcttg cggttttttc ggttcaatca
    17041 ccgcctgctg acgagctggg cgcgtagtgg acggacgttc agcggtttgc gttttcgccg
    17101 tgtcacgcga tgcattgcca ttgcgaacag gcgcgacagt cgcgggttcg gtcggcaatg
    17161 tggaattgac cgccacattg ttcaactgtt gctgattttg tggctgggtc agcgcattgt
    17221 tcaggtcacc ctgcacttca acacgttgtt gaccatccgt tgccgccggg gtttgccctt
    17281 gagtcggcgt agaagagatc ggtggcagag aaacatcctg ctgagtattt tccgcagacg
    17341 tggttcccgg cgctggctgc acgccattcg cctgatcggt cgcattgcct gcaagatcaa
    17401 tactcttctc gccagacgcg gtttgatcgc tggaagaggt cgagggggct tttagcgcag
    17461 aaccgatacc gatgatcaac agcagtagaa ccagaatgcc gacgcccatc atcatatact
    17521 gacgagaagc gggtttactg gctgcttttt tgcgcttacg cggacgacgc tctacgcgct
    17581 cttcatccac ggtttcatct tcggattcgt caatttcttc ttcgatttcc ggttcctcat
    17641 tgcgctcttt ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt tcaatatcat
    17701 caaaattgat ctgcggttcg ccacgttcag tacgctcaga acgttcagaa gattgacgag
    17761 aacgaccagt acgacgatcg ctgggatcgg gtttcagctc gtcttctggt ttgaattcat
    17821 ccatttaaca ccccactaaa aggttaatgc ttaccacgtt gcaattaacc tgaagctaat
    17881 agaccgcttg ataagcggcc tgacctttct tgttgttacg ctgattgaca atcggcaatg
    17941 gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt cactcttacc aattgccaac
    18001 ggaagaatta agcgtatctc tcccgcaagg actttcttgt cacgcagcat atgcggtaaa
    18061 tacgcctgcg cggacatttc gcgcggccca ttgaccggta acccagcccg cttgagcagg
    18121 gttataatac gctgcgtttc ggcagaacta aactgcccga gacgttccga cgtccgcgcc
    18181 gccatcacca tacccgcagc gaccgcttca ccatgtaacc aattgccata ccccatttca
    18241 gcttcaatgg catgaccaaa ggtgtgtccc agattcagta aagcacgtaa cccggtttcg
    18301 cgctcgtcgg cggcgacaac ttctgccttc agttcacaac aacggcgaat acagtacgcc
    18361 attgccggac cgtccagacg caacaacgca tccagattct cttccagcca gttaaaaaac
    18421 gcaccgtcaa gaataatgcc gtatttgatg acttctgcca gccccgacgc taactcacgc
    18481 gggggaagcg ttttcaggca gtcgagatcc accaccactg aagcaggctg gtagaacgcg
    18541 ccaatcatgt ttttaccgag gggatggttg accgcagttt tgccgccaac ggaggaatcg
    18601 acctgcgaca gtaacgtcgt cgggacttga atgaaacgaa caccgcgctg ataactcgcc
    18661 gccgcgaagc cggtcagatc gcccactacg ccgccgccaa gcgccaccag cgtagtatcg
    18721 cgaccatgcg gcttttgtaa caacgccgta aagacggtat cgagtacagc caggctttta
    18781 tactgctcgc cgtcagggag gataacgcta tcgacgttaa cacccgcctg ttcaagtacg
    18841 ccgcggacct tatcgagata cagaggagcc agggtttcgt tggtgaccaa catgacctgc
    18901 tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa acaaaccaga tgcgatggta
    18961 attgggtaac tacgttcccc gagagtaacg acaatcctct ccataacgcg acatccacct
    19021 taattactgt acccgcagac gagtgtatat aaagccagaa ttagttgctt tccagcatgt
    19081 gaataatctg gtttgcaacc actttagcgc tttgatcatc agtacgaatg gtcacgtcgg
    19141 caatctcttc atacagcgga ttgcgttcat tggccaacgc ttccagaact tcacgcggcg
    19201 gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca agttgctttt
    19261 cgatggtcgt ttcaagataa acgacaacgc cacgagcgga aagacggtta cgcgtttcac
    19321 gggatttcac agagccgccg ccagtagcca gcacaatacc ctgtttctcg gtcaactcat
    19381 tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg aaaacccagc
    19441 ccacatcagc tccggttcgt ttctcaatct cttgatcgga atcgtaaaat tccatattga
    19501 gttgttgagc taactggcgc ccaatagtgc tttttccggc acccataggc ccaaccagaa
    19561 agatattgcg tttctctgcc attttttcgg tactactaag actattcgtt aatgataaac
    19621 ccgcttcgct cagagagcgc cgcaggacat gaactgaaac ctcataagat attgcgagag
    19681 tcagactgaa aattatctca atactccagc gggtttggca actgaataaa tcacccatcc
    19741 ctctgcatat ctggtcgctg caagcgcgct gccttgctac caccgctctg gcgataaatc
    19801 accgggtaag attagcgtaa aaaagacagc aaaatgccgc ctgaatgata aatcatcatc
    19861 atggaacgca ggcagcgaag agcaccaact ctcaaatcgg tactccttgt atgctaaata
    19921 cctgcgcgcg tcaaatagat gaaacacgtt cagcataaaa acattaccgc ttttacggct
    19981 gtttactcac tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg tcgttcatct
    20041 tcttttccgt catgacgaaa taattgcccg aaccagggaa tgtcgccaag caacggtacg
    20101 ctatcctgac ccgatttatt tttacgggta aaaatgccgc ccagcgccaa cgtttctccg
    20161 cttttgacct cgacctgcgt ttcgatctcc tgcttatcaa tcgccagcac ttcgccatcg
    20221 gcctgctgta gcacctgccc cggaacgttc tggctgatgt gtaatttcag ccggatgcga
    20281 cctttttgta acaccgtggg cgtgacctcc atccccagga cggcctcttt aaattccacc
    20341 gacgtcgcgc cactttcccc gctggaaacc tgatatggaa tttcgctccc ctgtttaatg
    20401 ctggcaggct gaagatgtga ggccagcaga cgcggactgg cgataatatc cagctgctgt
    20461 ttttgttcga gcgcggaaag ctcaagatcc agcaagcgtc cgttgatgcg cccaatgtta
    20521 aaaccgacat gcgttgtcgc cgtcgctacg gagaggtcgc taccaagcgt ggtgacttgc
    20581 ccaacgccac cagcgtgttg cgcatcggcc agcgtccatt tcacgcctaa ctcacgcaaa
    20641 cttttttcat taatggtgac aatatgcgcc gacagctcaa cctgcccgac cggcagatcc
    20701 atttgcgcta cccactgttc aagcgcgctt aacgccgttt tgttatctcg tagcaaaagg
    20761 cgattggtgc gtttatcgac ggtcatactc cctttggcac tcagtagctt ctcccccgct
    20821 ttcgccagtt ctcccgcgtc ggcgtattgc agggttatac tgcgattttc cagcggcaga
    20881 tttgcctgcg cccgcgcctg ctccgcctcc tggcgggcga tattgttatt ctgccaggca
    20941 atggaatgca ctgagagaat gttgccttcc tgccgcgtta tcagtccggc gcttttcact
    21001 acagtttgta gtgcctgctt ccagggaaca tctgttagat gtaacgacac cgtaccgctg
    21061 acgtctggcg acacgaccag gttcaacttc tcctgttcag ccagcgcctg caacacctga
    21121 gctaccggaa cgtcatccac catcagcgtc actttttgcg gctttgccgc ctgtacgccg
    21181 ggtatcagca tcaacagtag tgcggctatc cattgcttca tttgtatctc cttgccgttg
    21241 ccacaaccat tgtggcggtt cgcagtttgt cccggttccc agcgttagtg cgtctggcgt
    21301 cagctgtaaa attgtccagc cgttttccag cacatcgttt tgctgcaccc gtcgccattt
    21361 cttttgcccg tcttttatta caccgatgat gtgctcgcct cgccctacca tcccctgata
    21421 gcgccactgg ctaagttcgc taatccggca tagatcttcc ggcggtttaa aagggtcacg
    21481 cataccggtt aaaaggcaca atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg
    21541 gtgtctccag ttgtagcgtg aacaaaagat catcaccttc cacgcttaac gaaaaacggc
    21601 tcacgctgac gttgcgctct gccagccgtg taaatgccga cggcactgct tcccacagcg
    21661 ttttcaacgc caactcacct ccctgcgcgg atggatgcca agaaaccagt tgcgcgccgg
    21721 ataactgaaa atccagaggc gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt
    21781 ctaccaggcg atacagtgcg gcccactgct ggtgatgact tgcccgtagt cgaattaatg
    21841 cgtcgctctc ttcatggtgt gtcgatgaca gaaaaatgag cgtaactaac atcagcaacc
    21901 agactgccca gcaaaactgg cggaggcggg gtgatatggc gaaccaccag tcaaagaaca
    21961 tgttcatcgc taaccttcct tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc
    22021 tgctgcgtgg ccccccgctg attgagatga aaagaagcat cctggcgaag tgacgtttct
    22081 agtgcgttta acgcggtaat gcttgttgta agccccttga tctccagcgt tccctgctgc
    22141 cagcttatcg ttgtcagcca ggcgtgttca ggtaaaagag ccgccagtgc ttccagcgca
    22201 gattgccagt cgcgggtaaa ttgtcgctgg cgctgccttt gcaaacgctg ctcacgaatt
    22261 tgttggcgct ccagcaaacg tggtttcgtt atctgtaagc tgtgggcgag ttgttgttcc
    22321 ccctgaagca aaacggcgtc tacgcgagct tcggcgctgc ttgtcagacg cagtattagc
    22381 gttatcccga cggccagcag cagaggcgca acgaacatca gcaaccagaa acgcagaaaa
    22441 gcggtccggc gttgctgtcg ccagggcaaa aaattaattg gcaggttcat cagtacgcct
    22501 tcccaagcgc cagccccagc gcgatggcaa agtctccacc tggcggcggt agcggcggct
    22561 gacgaacaga aacggcctcc cagggatcaa atccgccttc gccacatatc gcgacgctgt
    22621 cgggatcaac ggataacgct gccgccagct ccttcgcgct agtcatccct accgccagtt
    22681 tgcgccccca gcgatagcgt gtcgcccaca gccactgttc gttatcacgc cagaccagac
    22741 attgctgatg agaaggtaaa aaaggcagga attgctgtaa tgcactggca tccggggtga
    22801 tcgcgctcac atgaacacgc aacctttctg ccagagtaag tagcgttgcc agctctttgc
    22861 tttgcgcggc agtcacgtta taggcggggc tgagtgagtc ttcgctgtaa tcgaagcgca
    22921 gggagtccgg atccatatcc agctcgcggg ccatcgtccc tgacagccag gccgtttgct
    22981 cccgctcacc aagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg
    23041 gaaacgccaa cataatgtga tgacgctgcg gcagttcgcg actccacggt aacaacgttt
    23101 tagccagctg ctgcgcatca acaatccgcc catctttgat aatgtcgttc tccagcggca
    23161 accgccacca gcgttgcaaa aagcattctt ttgctccccg cacgatcgca accgctaccg
    23221 cttcttgctg ttgtaaatgc aaaccaattt gccagatctt aaatgccatt gtgatgatct
    23281 ccttatcacc cgtcactctg acgggtatat caatgcgtct ggcttgcctt tatactaccg
    23341 cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga aatttccagt gaagttcgta
    23401 aagtattttt tgatccttgc agtctgttgc attctgctgg gagcaggctc gatttatggc
    23461 ctataccgct acatcgagcc acaactgccg gatgtggcga cattaaaaga tgttcgcctg
    23521 caaattccga tgcagattta cagcgccgat ggcgagctga ttgctcaata cggtgagaaa
    23581 cgtcgtattc cggttacgtt ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg
    23641 acggaagaca gccgcttcta cgagcatcac ggcgttgacc cggtggggat cttccgtgcg
    23701 gcaagtgtgg cgctgttctc cggccacgcg tcacaagggg cgagtaccat tacccagcag
    23761 ctggcgagaa atttcttcct cagtccagaa cgcacgctga tgcgtaagat taaggaagtc
    23821 ttcctcgcga ttcgcattga acagttgctg acgaaagacg agatcctcga gctttatctg
    23881 aacaagattt acctcggtta ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc
    23941 ggtaaaacgg tcgaccaact gacgctgaac gaaatggctg tgatcgccgg gctgccgaaa
    24001 gcgccttcca cctttaatcc gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc
    24061 gtactgtcgc ggatgctgga tgaagggtat atcacccaac aacagttcga tcagacacgc
    24121 actgaggcga ttaacgctaa ctatcacgcg ccggagattg ctttctctgc gccgtacctg
    24181 agcgaaatgg tgcgccagga gatgtataac cgttatggcg aaagtgccta tgaagacggt
    24241 tatcgcattt acaccaccat cacccgcaaa gtgcagcagg ccgcacagca ggcggtacgt
    24301 aataacgtgc tggactacga catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg
    24361 aaagtgggcg agtcggcgtg ggataacaac aagattaccg atacgctgaa ggcgctgcca
    24421 acctatggtc cgctgctgcc tgccgcagtc accagcgcca atcctcagca agcgacggcg
    24481 atgctggcgg acgggtcgac cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct
    24541 taccgttcgg atactcagca aggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg
    24601 ggtcagcaaa tctgggttcg tcaggttggc gatgcatggt ggctggcaca agtgccggaa
    24661 gtgaactcgg cgctggtgtc gatcaatccg caaaacggtg ccgttatggc gctggtcggt
    24721 ggctttgatt tcaatcagag caagtttaac cgcgccaccc aggcactgcg tcaggtgggt
    24781 tccaacatca aaccgttcct ctacaccgcg gcgatggata aaggtctgac gctggcaagt
    24841 atgttgaacg atgtgccaat ttctcgctgg gatgcaggtg ccggttctga ctggcagccg
    24901 aagaactcac caccgcagta tgctggtcca attcgcttac gtcaggggct gggtcagtcg
    24961 aaaaacgtgg tgatggtacg cgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat
    25021 ctgcaacgct tcggcttccc ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt
    25081 tcagcgtcct tcaccccaat gcaggtggcg cgcggctacg cggtcatggc gaacggtggc
    25141 ttcctggtgg acccgtggtt tatcagcaaa attgaaaacg atcagggcgg cgtgattttc
    25201 gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc cggtgattta cggtgatacg
    25261 cagaaatcga acgtgctgga aaataacgat gttgaagatg tcgctatctc ccgcgagcag
    25321 cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag
    25381 actggcgcgc aggagtacgc accgcacgtc atcaacactc cgctggcatt cctgattaag
    25441 agtgctttga acaccaatat ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca
    25501 ggtcgtgatt tgcagcgtcg cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa
    25561 gatgcgtggt tctcgggtta cggtccgggc gttgtgacct cggtctggat tggctttgat
    25621 gatcaccgtc gtaatctcgg tcatacaacg gcttccggag cgattaaaga tcagatctca
    25681 ggttacgaag gcggtgccaa gagtgcccag cctgcatggg acgcttatat gaaagccgtt
    25741 cttgaaggtg tgccggagca gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc
    25801 gatcgcagca ccgggcagtt agctaatggt ggcaacagcc gcgaagagta tttcatcgaa
    25861 ggtacgcagc cgacacaaca ggcggtgcac gaggtgggaa cgaccattat cgataatggt
    25921 gaggcacagg aattgttctg attaaaaagg cgcttcggcg ccttttcagt tggctgacaa
    25981 agtgcatttg tttatgccgg atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa
    26041 aatcaatata ttgcaggaac cccataagcc tgataagcgt tgtgcatcag gcaaacttca
    26101 cgcatttaca ctcgcccctg ccctttcaac cattcgcgca cgaggaacag cgcgctgaca
    26161 ttacgcgctt cgttgaagtc agggtcttcc agcaaatcca tcatatgcgc cagcggccag
    26221 cgcacctgtg gtagcggctc tggctcatcg ccttccagcg attccgggta gagatcttgc
    26281 gcgaccacga tattcatttt gctggaaaag taagacggtg ccatgctgag cttcttcaaa
    26341 aaagtcagat cgttcgcacc aaatccaacc tcttctttta gctcgcggtt agcggcttcg
    26401 tagacgcttt cacccggatc aattaatcct ttcgaaaaac ctaattcgta ggattcagtt
    26461 cccactgcgt attcgcggat caggatcagg tgatcgtcca caatcggcaa aatcatcact
    26521 gcttcccggt tggttggacg cattcgttca taaacacgcc gcacgccatt gctgaactcc
    26581 agatccacgc tctcgacggt aaacagtcgg gaacgggcta cagtttcaac attcagaatg
    26641 gtgggttttt gtaatgattt gctcatcgtg ggatctatgc tgtgaaatca gcggttattg
    26701 tgcgatatcg gacacgcttt cggcaatgtg aattgcatgt tatttacatt tatgtaactt
    26761 aataaataat cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac tccaggaatt
    26821 tgctgatatt ccgccttcag agggtttgct atgatcagcg gttactgtga tgtgcttaat
    26881 gatgctcaag ttaaactcca cgcttgccga tagccaaccg cagaatcatg tattgtgtct
    26941 ggtgcgactg accacgcctg acagactaag taagatgggg aaagcatgag caccattgtg
    27001 atttttttag ctgctttgct ggcctgctca ctacttgcgg gatggctgat aaaagtgcga
    27061 tccagacggc gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc
    27121 acacctgaag aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca ggtattacag
    27181 gtgcctggcc caacgggagc tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc
    27241 aacaacgtca tgatgctgac acacgctatc acgcgttacg gcatctctac cgacgatccg
    27301 aataaatggc gttactacct cgattcggta gaagtccacc tgcccccttt ctgggaacaa
    27361 tacatcaacg atgagaatac cgttgaactg atttataccg attcgctgcc gctggttatt
    27421 tcactcaacg gtcatacgct gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa
    27481 cctgttccgt ccacgcaggc gtcgattcgc ggggaagaga gtgagcaaat cgagctactc
    27541 aatattcgca aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa
    27601 gcgttgctga tcgtcgcctc cttcctgatg ttctttttct gcctgattac cccggatgta
    27661 tttgttccgt ggctggcagg cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta
    27721 ttcgcgcccc cggcaaaatc ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt
    27781 cgttggggat tgtttggcga aaacgatcag gaacagatca acaatatttc actcggtatt
    27841 atcgacctgg tctatcccgc acactggcag ccatacattg ctcaggatct cggtcaacaa
    27901 accgatattg atatctatct cgaccgccac gtagtgcgtc aggggcgata tctttcgctg
    27961 catgatgaag taaaaaactt tccgttacag cactggctgc gcagtacgat tatcgctgcg
    28021 ggctcgctgc tggtgctgtt tatgctgtta ttctggatcc cgctggatat gccgctgaaa
    28081 ttcactctct catggatgaa aggcgcgcag accattgaag ccaccagcgt aaaacaactg
    28141 gctgacgctg gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg aatgtgtaat
    28201 attcgcactt ccggaacctg gagcgcgaaa accaattcac cttttttacc gtttgactgc
    28261 tcgcagatca tctggaacga cgcccgctca ttaccattac cagaatctga actggtcaac
    28321 aaagcgacgg cattgaccga agcagttaat cgccagctgc accctaaacc ggaagatgaa
    28381 tctcgcgtca gtgcctcatt acgttcagca attcaaaaat ccggcatggt attgcttgat
    28441 gattttggcg acattgtact gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg
    28501 cgactgaaaa atgcgctggt caatctcggc aacagtaaag actgggacgc gctggtaaaa
    28561 cgcgccaacg ccgggaagct cgatggcgtg aatgtgttat tacgcccggt gagtgcggaa
    28621 tcgctggata acctggtggc aacctccacc gcgccgttta tcacgcatga aacggcgcga
    28681 gcggcacaat cactaaacag tccggccccc ggcggattcc tgattgtcag cgacgaaggc
    28741 agcgattttg ttgatcagcc ctggccttcg gcatcacttt acgactaccc gccgcaagaa
    28801 cagtggaacg ctttccagaa actggcacaa atgctgatgc atacgccgtt taacgccgaa
    28861 ggtatcgtca caaaaatctt cactgacgcc aatggtacgc agcatattgg ccttcatccg
    28921 atcccggatc gttccggcct gtggcgctat ctcagcacca cattgctgct actgacgatg
    28981 ctgggtagcg ccatttacaa tggcgtacag gcctggcgtc gttaccagcg tcatcgcact
    29041 cgcatgatgg agattcaggc ctattatgaa agctgcctga acccgcaact gatcacccct
    29101 tcagaaagcc ttatcgaata acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa
    29161 ttgcttcact ctggagattt ccctcatgca tatcaacatt gcctggcagg acgtagatac
    29221 cgttctgctg gatatggacg gcacgttgct cgacctcgcc ttcgataact atttctggca
    29281 aaagctggtg cctgaaacat ggggcgcgaa aaacggggtt acgccacagg aagcgatgga
    29341 atatatgcgc cagcaatatc acgacgtaca gcatacgcta aactggtact gtcttgatta
    29401 ctggagtgag caactgggtc tggatatcta tgcgatgacc accgagatgg gaccgcgcgc
    29461 cgtactgcgt gaagatacca ttccgtttct tgaggcactg aaagccagcg gtaagcagcg
    29521 aattttgctc accaatgctc atccgcacaa cctggcggta aaacttgagc atacgggtct
    29581 ggacgcacac cttgatttgt tactttccac ccacacattt ggttatccga aagaggatca
    29641 gcggttatgg catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat
    29701 tgatgacagc gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg
    29761 cgtgactaat cctgattccg ggattgccga gaaacagtat caacgccatc cgtcactgaa
    29821 tgactaccgc cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa gaaaaacctg
    29881 ctgttgaggt tcgactggat aaatggctat gggctgcccg tttttataaa acccgcacgc
    29941 tggcccgtga aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc agcaagccga
    30001 gcaaaatcgt cgagctgaat gccacgctca ctctgcgcca gggaaatgac gaacgcacgg
    30061 tgattgtaaa ggcgattact gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg
    30121 aagagactgc ggaaagtgta gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg
    30181 ccttaaccat gccgcacccg gaccgacgcc cggacaaaaa agagcgccgc gacctgttac
    30241 gatttaaaca cggcgacagt gaataactgt cacctgcaag agagatgatt atgccgcaac
    30301 atgaccaatt acatcgctat ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg
    30361 tttcggaaac cctgcaacag atccttgaga accacgatta tccgcagccc gtcaaaaacg
    30421 tgctggcaga actgctggtt gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg
    30481 atatcaccgt acagctgcag ggcgacggtc cgatgaatct ggcggttatt aacggtaaca
    30541 ataaccagca aatgcgcggt gtggcgcgcg tgcagggcga aattccagaa aatgccgacc
    30601 tgaaaacgct ggtcggcaat ggttacgtgg tgatcaccat taccccgagc gaaggcgaac
    30661 gctatcaggg cgtagttggt ctggaaggtg ataccctggc ggcctgcctg gaagattact
    30721 ttatgcgttc tgaacagctg ccgacgcgcc tgtttattcg caccggcgac gtagacggca
    30781 aaccggctgc aggcggtatg ttgttgcagg taatgcctgc gcaaaatgcc cagcaggacg
    30841 actttgacca cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac
    30901 cggcaaacga agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc
    30961 aggatgtgga gttcaaatgc acctgctcgc gtgaacgttg cgccgacgcg ctgaaaacgc
    31021 tgcctgatga agaagtcgat agcatcctgg cggaagatgg cgaaattgac atgcattgtg
    31081 attactgcgg taaccactat ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg
    31141 cgtctccggc agatccgcaa gttcattaat gagtatgtcc ggcagagagg gctctgccgg
    31201 attttacgac gtaatggcct gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa
    31261 attattttgg tcagatacag cgtcgtatcc gataaaagcg ttgtcactta cttaagttta
    31321 tttccagcgt ttcaccatct accgtcagat cgcgccacgc tttcggcctc gtccccagtt
    31381 ttccatcctt cgcagctgtg agtaattttt ctttcgttat ttcgttcgga agtaaactca
    31441 tatcaagcgc gtcccattct tttctgtctg agtcaaagcc atagacaatg actctgtcat
    31501 cattaaaagc aaacagaatc cgctccgcct gaccatcact attcagatct tgctcgacca
    31561 gtacgcaggc gtctttttca atacaggtca tcacgttata gcgatcctgg attaaggccg
    31621 accagaatgt cgcatccggt ttaacagaac caggggcaat taacacattt tcggctaata
    31681 ctttttccga tacctgttcc tgaagatgtt gctctccatc taacgccatc agcagatccc
    31741 gcgcgcgttt cgggtctttc ataaacccgg catcgctttt cagcgactca agcgccgcac
    31801 gtccatagcg accgctctgt tcgagcatgt agatagttac ctggtctggc gtgtttttgc
    31861 cgctctgata acgcgccata tgactgttca cgctaatgcg catactgtcc agcaccggcg
    31921 aattaagaag caccagtatc accaacacca ataacgaaac cgcaaggttc actttgccct
    31981 gaagaacaag gggattttgc ccattacgcc agacgatact gacaaaatac cccagcgacc
    32041 acaccagtaa caccagcacc gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact
    32101 gagcgacccg cagccataac gcccaggcgg cgacgaacac atacagcgga gcaaccagca
    32161 aagcggtttt aatcaggcaa cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat
    32221 cgcggacaat agccattaag atcaattgca aaaaggccag cgttaacagc aacccggcgg
    32281 cggagatgtg gcgagaaatc gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca
    32341 gggttagcaa tgatactaac ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa
    32401 tagagtcgat taaacgtgac tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac
    32461 ctaatgtaag atacataaac cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc
    32521 caacaagttt aaacaactca ccccacagta aaagcaccag ccacgtcagg ccattggcga
    32581 gaaaaatcac cagtaatata agtacgttat gccatactga ctggtaaaaa tagcgatagc
    32641 ggctactgtc attgcggatg cgaaggcttt gttgtatcca tggtagcaac aacattgcca
    32701 tcagcagcag atagcaacca aaatcccaaa gcgccttttc agctctccag gggttcatgc
    32761 catcagtttg ccacttcaac cagccgctca tccccagcgt ggcaataaac accagcgcca
    32821 gccatcccca aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa
    32881 acgcaacggt cgcaggcacg ccataaacaa tccaactgtg attttttccg gcaagccagg
    32941 cgatcaacag gtagcaaacc agcccctgaa gtaatccggt agcaatcatc ccccagcgtg
    33001 ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat
    33061 tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa aagttagcgt
    33121 ggtgaatcga tactttaccg attgaatttg catcaatttc attaaggaat gcgattccac
    33181 tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt tccacaccta
    33241 aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc
    33301 aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa cacccccaaa
    33361 aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc gcgttaacaa
    33421 tggtttgacc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg atatcgttta
    33481 caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga caggttatga
    33541 gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct tcaccggtcg
    33601 ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt tctggtgggc
    33661 agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct ggcagcatct
    33721 gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg acgctttctg
    33781 tggtgcgaat ccggatactc gtctttccgt ccgtttcatc accgaagtgg cctggcaggc
    33841 gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag gtttcaaacc
    33901 agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag aacagggtct
    33961 gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga ttggcggcac
    34021 ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc tgctgccgct
    34081 gaaaggtatc gcttctatgc actgctccgc caacgttggt gagaaaggcg atgttgcggt
    34141 gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga aacgtcgcct
    34201 gattggtgat gacgagcacg gctgggacga tgacggcgtg tttaacttcg aaggcggctg
    34261 ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca acgctatccg
    34321 tcgcgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg actttgatga
    34381 tggttcaaaa accgagaaca cccgcgtttc ttatccgatc tatcacatcg ataacattgt
    34441 taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg ctgatgcttt
    34501 cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc acttcctctc
    34561 tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga cgccaacctt
    34621 ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg gctcagtacg cagaagtgct
    34681 ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg gctggaacgg
    34741 cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg
    34801 ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga tcccaaccga
    34861 actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt ctccggaaca
    34921 gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg ataaatacac
    34981 cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat gatttgaagc
    35041 tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt
    35101 ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcactggc agccaggcgc
    35161 gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta tggttatcca
    35221 cgatacgctg cacaattgcc agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc
    35281 cgcggacaaa cggctggaac aggtgcttac gttgttccgg cgcaattccc ggaccgtcat
    35341 cttccacctg gaaccaggcg cgattcggct ccgttccgct gctgactttg atccagccat
    35401 tgccataacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc gacagcgggt
    35461 gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc tcatagccac
    35521 tttcggcagc aatcacctca ccgagtactg cattaagatc cgccatttcc atcggcatct
    35581 cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac tcttcgatat
    35641 ctttattgat cgattctgcc agatagccat cctgctcgct catcatctca gtcgccaggc
    35701 gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt
    35761 catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcacgggta acggaacgca
    35821 cctccgaagc gccatactca cgcagcggcg gcggaataat ccctttacca acctgcaagg
    35881 ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc cacgccccgc
    35941 ctatcgccaa tagcataatc gccagcgtat agcggaacag cggagagaaa tcgccctgat
    36001 gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt ttcagccaga
    36061 cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc agttgctgtg
    36121 ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag
    36181 cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg aaagcgggag
    36241 gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac atacgcactt
    36301 cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag ttcagcacca
    36361 ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg
    36421 caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa
    36481 gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat cttcttctac
    36541 catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg aatattcacg
    36601 accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat ggctgaccag
    36661 tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcatctt cgcggaacat
    36721 ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct cttcctgtga
    36781 cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac gggccagcag
    36841 ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca ggcctacgat
    36901 acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt tgctctgact
    36961 acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat ccagtaccat
    37021 aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag cgacgcttcg
    37081 aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc
    37141 atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag gttcgcaaca
    37201 atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa
    37261 tttcagccta aattgttaca aagcatatta aacagcagct taagtataca atttattcgg
    37321 cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc attatcaaac
    37381 agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac
    37441 tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga
    37501 cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat aataaaaagc
    37561 gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa aatctcaaaa
    37621 tcgtcgatta ctcccctcag caggaaggca aagtcttttt tggcgcgtgg gtggagattg
    37681 aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa atttttggcc
    37741 gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa aaagaagtcg
    37801 gcgatctggc ggtggtgaat acccctgccg gggaagcgag ctggtatgtt aatgctatcg
    37861 agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca tttttgccag
    37921 ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa accatgatga
    37981 atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa caggttgacg
    38041 ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg
    38101 aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc
    38161 tgcgcgagct ggaagagaga cgtcaggcga tcctcaagtc catttccgag caaggcaaac
    38221 tcaccgatga tctggcgaag gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc
    38281 tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt gaagcagggc
    38341 ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg
    38401 cacaatatgt tgatgccgat aaaggcgtgg cagataccaa agccgcgctg gatggcgcgc
    38461 gctatatcct gatggaacgg tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt
    38521 atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa gaggaagggg
    38581 cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg
    38641 cgctggcgat gttccgtggg cgtaacgaag gcgtactcca gctttcgctg aatgccgatc
    38701 cgcagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg gatcaccttg
    38761 gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aggcgtagtg agctggacct
    38821 ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggtaccgtg cgcgaacgcg
    38881 cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg atggcggccc
    38941 ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtactggg gtaaaagtgg
    39001 cggtggtcga tgccactggc aaactggtag cgaccgatac catttacccg cacaccggac
    39061 aagccgcaaa agcagcgatg actgttgctg cgctgtgtga aaagcataac gttgaactgg
    39121 tggcgatcgg caacggtaca gcttcccgcg aaaccgaacg tttctatctc gacgtgcaga
    39181 agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt
    39241 actcggcttc cgagctggca gcgcaggagt ttccagatct cgacgtttcg ctgcgtggcg
    39301 cggtgtctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa atcgatccga
    39361 aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg gcccgcaaac
    39421 tggacgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac actgcttctg
    39481 ttccgctgtt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct
    39541 ggcgcgatga gaacggccag ttcaagaacc gtcagcaact gctgaaagtc agccgtctgg
    39601 ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt gataacccgc
    39661 tggacgcctc taccgttcac ccggaagcct atccggtggt ggaacgcatt ctggcagcaa
    39721 cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac ctgaaagcgt
    39781 ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg
    39841 aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg
    39901 agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgctgtcacc aacgtcacca
    39961 actttggcgc gtttgtcgat attggcgtgc atcaggacgg cctggttcac atctcttcat
    40021 tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac attgtgaagg
    40081 tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgaccatg cgtctggacg
    40141 agcagcctgg cgaaaccaac gcccgtcgcg gcggcggtaa tgaacgcccg caaaacaacc
    40201 gcccggcagc caaaccgcgc ggacgtgaag cgcagtctgc cggtaacagc gcgatgatgg
    40261 acgcgctggc ggcagcaatg ggcaaaaaac gttaaacgcc ctgattgacc cctaaacctg
    40321 cgcgataagg tgtccacgcc gcatctggca acgaacgcct tgtctgacat aaaagtgccg
    40381 gagaatatct ccggcatttt tattccacag ccaaactcac aatatattcc ggcaacattt
    40441 atcacttcat taacaactga aaccttaatt aaacattagc cagtcctggt aattcgctat
    40501 tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag tcagattatt
    40561 attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt ctcattatca
    40621 ttatgctgtt gattatttaa tctctccttc gttggcaaat catctggtct catgtcgctg
    40681 tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac ctatgcaata
    40741 cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc cggcatatcg
    40801 ccaaaaactg ctttctcttg gcatgttacc tggctcctct tttaatgtgg tgcgcgtcgc
    40861 tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa
    40921 agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata acaacaatga
    40981 aaaaattaac cataggctta attggtaatc caaattctgg caagacaacc ttatttaacc
    41041 agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag
    41101 aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc ggcacctatt
    41161 ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt cactacattt
    41221 tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt gagcgtaacc
    41281 tgtacctgac gctacaactg ctggaactcg gcattccctg catcgtggca ctgaacatgc
    41341 tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctgtcg gcgcgtcttg
    41401 gctgcccagt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg
    41461 cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca cagccgctac
    41521 tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg aaacaacgtc
    41581 gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac gccggtgaag
    41641 cgtcgcagca tctggatgcc gccctcgccc gtctgcgtaa tgagatggac gatccggcgc
    41701 tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca
    41761 ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt
    41821 tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg gctatcaaca
    41881 tcggcggggc gttacagccg ctgtttgacg tcggctccgt ggcgctattt gtgcatggta
    41941 ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc ctcgcccagg
    42001 gcctgggtgg cggcattaac accgtgctgc cactggtgcc gcagattggc atgatgtacc
    42061 tgttcctctc cttccttgag gactccgggt atatggcgcg tgcggcgttt gtgatggacc
    42121 gtctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc gtcggtttcg
    42181 gttgtaacgt accgtcggta atgggtgcac gtacgcttga tgcaccgcgt gaacgtctga
    42241 tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat
    42301 tcgcggctgc cttcttcggg cagaacggtg cgctggcggt cttctcgctg tatatgctgg
    42361 gtattgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg cgcggtgaag
    42421 cgacgccgtt tgtcatggag ttgccggtct atcatgtacc acacgttaaa agcctgatta
    42481 tccagacctg gcagcgtctg aaaggcttcg ttctgcgtgc tggtaaagtg atcatcatcg
    42541 tcagcatttt cctgagcgct ttcaacagct tctcgctgag cgggaaaatc gtcgataaca
    42601 tcaacgactc ggcgctggcg tccgtcagcc gggtgatcac cccggtcttc aagccaattg
    42661 gcgtgcatga agataactgg caggcaacgg ttggcctgtt tacaggtgcc atggcgaaag
    42721 aagtggtagt gggtacgctc aacaccctct acaccgcaga aaatattcag gacgaagagt
    42781 tcaatccggc agagtttaac ctcggtgaag agctgttcag tgcgatagat gaaacctggc
    42841 agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa gccagcaaag
    42901 gcgacggcga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag
    42961 cagcagctta cagctacctg attttcgtcc tgctgtatgt accatgtatc tcggtgatgg
    43021 gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg tgggggctga
    43081 atatcgctta ctcgctggca acattgttct atcaggtcgc cagctacagt cagcacccaa
    43141 cttacagcct ggtgtgcatt ctggcggtta tcctgtttaa catcgtggtt atcggtctgc
    43201 tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag tcggtaagca
    43261 gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta ttcaggtgcg
    43321 cgatttgctg gcgttacggg gccgtatgga agcggcccag ataagccaga cattgaacac
    43381 tccacagcca atgattaacg ccatgctgca acaactggaa agtatgggca aagccgtgcg
    43441 gattcaggaa gaacctgacg gctgcctctc tggcagttgt aaaagctgcc cggaaggaaa
    43501 agcctgtctg cgcgagtggt gggcgctgcg ttaaccttac tccatcgcct gttttggaaa
    43561 gcccggtatg cgtctgcatc cgggcttttt tgcgtgcggc tttccataaa aatgcaactc
    43621 ttgcagcacg gcgtaagttc ctttgaaagc atctcgcagg gatgaaaact cgctaataca
    43681 caggtgtgaa gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg aacgccatga
    43741 gcaaaaagca gagttccacc ccacacgatg cgctgttcaa actcttttta cgccaaccgg
    43801 acacggctcg tgattttctt gcgtttcatt taccggcacc cattcacgcg ctttgtgata
    43861 tgaaaaccct caagctggag tcgagcagct ttattgatga cgatctgcgt gaaagctatt
    43921 ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg atacatctat tgtctgattg
    43981 aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac gctattgccg
    44041 caatgcaaaa tcaccttgat gctggataca aaacgttgcc gatggtggtg ccattgttgt
    44101 tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat tgtttcgccg
    44161 atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat gtcaccgtca
    44221 tgcctgatga tgaaatcatg cagcaccgac gtatggcgct gctggagtta attcaaaaac
    44281 atattcgtca acgcgatctg atggggctgg tagagcaaat ggcctgctta ttaagtagtg
    44341 gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa accggcgacg
    44401 cggtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga
    44461 gtttaatgac aattgcggaa agattgcggc aggaggggga acaatccaaa gccctgcata
    44521 tagccaaaat aatgcttgaa tccggagtcc ctcttacaga catcatgcgc tttaccgggg
    44581 tgtcagaaga agagttggct gcggcgagcc agtaaatttc tgtctcgcca tttcaaaagt
    44641 tgcctacacc ctctgcttca acgccaccag caggtgacaa aactcgaccg gatgcgaaat
    44701 aaacggcgca tgggccgctt tggcgaagat atatgattcg ctgtgaggcc aaagtttatc
    44761 cagcatcggc accactttgc gcggcaccag accgtcgaga tagccataca atcgcaaaaa
    44821 cggcatgggc acgttttgca gcggctgacg gagatcgacc gttttcagga tttctagccc
    44881 gccattaagc acgtcaacct ccggcatcgg taacgccaga acggttttct tcaacgcccg
    44941 cgcatcctgg cgcgccgttt cagtccccat ggtttgtaac gccaggaacc gctccactgt
    45001 acgctgaaaa tcatcactga gttgctgctg aaatcccgcc agcacgtccg gttttatccc
    45061 cggccactcg tcacgagcac taaaacaagg tgacgacgcc acggtgacca gcgcctgaac
    45121 acgctcggga tgggttaacg caatctggct tgccaccagc ccgcccagac tccagcctaa
    45181 ccaaatggct ttatcaggtg cctgttgcag cacggcttcg gccatatcag caagtgacag
    45241 cgcaccaaat ccccggctac gcccgaagcc gggcaggtca acaaggtgca gcgtaaaatg
    45301 cgagctaagt tcctcgtcaa tgcaacgcca cacttcggca ttcagtcccc atccgtgcag
    45361 cagcacaaga tgaacattcc cctgaccttt ggtctgccac cagatgttat tcatccgcta
    45421 ttgttctctt ttgacttaca aggatgaaca tatgctaaca gtaccgggat tatgctggct
    45481 atgccgaatg ccactggcgt taggtcattg ggggatttgt tcggtctgtt cacgcgccac
    45541 ccgcacagat aaaacgttat gcccacaatg tggattaccc gccacacact cccatcttcc
    45601 ctgcggtcgc tgcctgcaaa aaccgccgcc ctggcaaaga ctggtcacgg ttgccgacta
    45661 tgcgccgccg ttaagtccgc ttatccacca gcttaaattt tcccggcgca gtgaaatcgc
    45721 cagcgccctg tcacgtctgt tactattgga agtcttacac gctcgtcgca ccaccgggtt
    45781 gcaattgccg gatcgcatca tcagcgttcc gttatggcag cggcgttact ggcgtcgggg
    45841 atttaatcag agcgatttgc tgtgtcagcc gttatcacgc tggttgcact gccaatggga
    45901 tagcgaagcc gtcacacgta cacgggccac tgcgacccag cattttctca gtgcccggct
    45961 gcgcaagcgc aacctgaaaa atgcctttcg tcttgaattg cccgtgcaag gtcgccatat
    46021 ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc gcagagattg cgcagttgct
    46081 tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt tgtcgaacct tgtagagcct
    46141 cgatgatggg cgtattataa ccaactaaaa tagtcaacta ttaggccatt actatgatcc
    46201 gtatttccga tgctgcacaa gcgcactttg ccaaactgct ggcaaatcag gaagaaggga
    46261 cacaaatccg cgtatttgtg attaaccctg gcacgcctaa cgctgaatgt ggtgtttctt
    46321 attgtccgcc ggatgccgtg gaagccaccg acactgccct gaaatttgac ctgctgaccg
    46381 cgtatgttga tgagttaagc gcaccatacc tggaagatgc agagatcgac tttgttaccg
    46441 accagttggg ttctcagtta acgctgaaag ccccgaacgc caaaatgcgt aaagtggcag
    46501 acgatgcacc gctgatggag cgcgtggagt atatgctgca gtcgcagatc aacccacagc
    46561 ttgctggtca cggtggtcgc gtttcgctga tggaaatcac cgaagacggt tacgccattc
    46621 tgcaatttgg cggcggctgt aacggttgtt ccatggtcga tgtgacgctg aaagaaggga
    46681 tcgagaagca gctgctgaac gaattcccgg agctgaaagg tgtgcgcgat ctcaccgaac
    46741 accagcgcgg cgaacactcc tactactaag ttatcccctc ttttgtagat tgcccgatgc
    46801 gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc cagacgttgg cacagcattg
    46861 ctttgacaaa tccctccctt cgtttccccg cgataatatg accaacctct cataatttaa
    46921 atttaccccg ctctagtgat tctcaaacgc cagatgttac ccgtatcatt cacatgggta
    46981 ccaaacatac tcctgacatc tgactacaat aattagtttt agtgggaatc agtcgtggtg
    47041 ccgcaatatc tctgttcccg attgggataa ttagagtttg tcgtcagaaa attgacgtta
    47101 cccataacaa atgaaaggcc aggtaaatca tgccattagt cattgttgct atcggtgtaa
    47161 tcttgttgtt gctcctgatg atccgcttca aaatgaacgg cttcatcgct ctcgtcctcg
    47221 tggcgcttgc tgttggatta atgcaaggaa tgccgctgga taaagttatt ggctccatca
    47281 aagccggtgt cggcgggacg ctcggtagcc ttgccctgat catgggtttt ggcgcaatgc
    47341 tgggcaaaat gctggcagac tgcggtggcg cacaacgtat cgccaccacg ctgattgcca
    47401 aatttggtaa aaagcacatc cagtgggcgg tggtgttaac cggttttacc gttggttttg
    47461 ccctgttcta tgaagtgggc ttcgtgctga tgctgccgct ggtgtttacc atcgcggctt
    47521 ccgcgaatat tccactgctg tatgttggtg taccaatggc ggctgcactg tctgtgaccc
    47581 acggcttcct gccaccgcat ccgggcccga ctgcgattgc caccattttc aatgccgata
    47641 tgggtaaaac cctgctgtac ggtactattc tggcaatccc gaccgtgatt ctcgccggtc
    47701 cggtttacgc tcgcgtgttg aaaggtatcg ataagccaat cccggaaggt ctctacagcg
    47761 cgaaaacctt cagcgaagaa gagatgccga gctttggcgt cagcgtctgg acttctctgg
    47821 tgccggttgt gctgatggcg atgcgtgcga ttgccgaaat gatcctgccg aaaggtcacg
    47881 ctttcctgcc ggtagcggag ttcctcggtg acccggtaat ggcaacgctg attgccgtgc
    47941 tgattgcgat gttcaccttt ggtctgaacc gtggtcgttc aatggatcag attaacgaca
    48001 cgctggtttc ttccatcaaa atcattgcga tgatgctgtt gatcatcggt ggtggcggtg
    48061 cgttcaagca ggtgctggta gacagcggcg tggacaaata cattgcttcc atgatgcacg
    48121 aaaccaacat ttctccgctg ctgatggcct ggtcgattgc tgccgtactg cgtatcgcgc
    48181 tgggttccgc aaccgttgcg gcaatcactg cgggtggtat cgcggcaccg ctgattgcaa
    48241 cgacgggtgt tagcccagag ctgatggtta ttgcggtggg ttccggtagt gtgattttct
    48301 ctcatgtgaa cgatccgggc ttctggctgt tcaaagagta ctttaacctg actatcggcg
    48361 agaccatcaa atcctggtcg atgctggaaa ccattatctc ggtgtgcggt ctggtaggct
    48421 gtctgctgct gaatatggtg atttgataac actgactgcc ggatgcggcg tgaccgcctt
    48481 atccggccta cgattcggga tgaattagta ggccggataa gacgcgtcaa gcatcgcatc
    48541 cggcatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct
    48601 tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg gaaagcttgc
    48661 gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca
    48721 gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca
    48781 aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
    48841 gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct
    48901 gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta
    48961 gatccccgct ctcccagtaa ccgcgtagcg taggcaggtc atgtgtcgcc gcaaccgcca
    49021 tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat
    49081 agagcacttt gtaggagtac acaccgctgc tgcgcagctt accgacaatc tctaccggta
    49141 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
    49201 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
    49261 acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt
    49321 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
    49381 gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata tccggcggcg
    49441 cgccaaccga cgctttcagg caatatagtt cacggtcaca ccaggtttcc gccccacctt
    49501 ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta
    49561 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
    49621 cgtcattacg atgttcttcg cagaactgac gcacttctgg tgaatccacg ttctgataca
    49681 tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
    49741 gcgcatcaaa ggctgcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
    49801 acgcggccat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
    49861 tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc gcctgttgca
    49921 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
    49981 cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
    50041 atgggctggc actctccgga tttgccggat agagcgcatg aatcgggttc aggccgatga
    50101 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
    50161 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
    50221 gtttattcag caacgcctgc ggttcgtaac agcgtttcgg ggcgacaatc acccggcaat
    50281 gcgcgcgctg gtcgtcctgg gtgagtgtca gcgtgtgata accttccggc agcttcgtcg
    50341 gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgtgttcct tcttcggtgg
    50401 tcagcagcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
    50461 tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca
    50521 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
    50581 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
    50641 tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
    50701 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
    50761 ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg aaatccgcca
    50821 tcaccagata cggatcgccg ccttgtttgc cgatactgtg cagcatctgg tcgaaggcat
    50881 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
    50941 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaattgcc ttcacttgtt
    51001 ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt
    51061 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
    51121 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
    51181 gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa
    51241 ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg
    51301 ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag
    51361 cctgcgggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct
    51421 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
    51481 caataccggt acgaactttc acaaactccg ccagacggac tttattcgcc tgcttgatct
    51541 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat
    51601 cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc agtgcagggt
    51661 tgcactgttt gatccagcga cgtggggtaa taccgttggt gacgttatgg aatttgttcg
    51721 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
    51781 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttat
    51841 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca
    51901 gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc agtaagcctt
    51961 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
    52021 cgaaagtttt gctggtaatg gcccaggcgt catcccagct catctggtgc tcatcgatca
    52081 gcacgcgcag cagttctgga atcgcgatag ttgggtgggt atcgttcagc tgaataactt
    52141 cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg
    52201 ctaccgaaca ggcacactgg aagtactgct gcatcaagcg cagcttttta ccggcagtat
    52261 ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgccc tgctgttcgg
    52321 cacgcaggaa atcaccgtcg ttaaatttag tcagatcaaa cggatgcgcg tgcgtcgcct
    52381 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
    52441 cttgaccggt aatagtaaac tccggctccc agcgtccgtc tttcgtcact ttaccgccaa
    52501 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc
    52561 agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
    52621 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgaggaagc
    52681 acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca
    52741 ggtccgtcag attgatgtca taagccttca acgaatcctg tacatcctga taccagccga
    52801 gattcaacag gttgttgccc gtcaggcgac caatcaaaaa ctccattgag atgtagttaa
    52861 catgtcgctg attcgccacc ggcttggcga atggctgagc acgcagcatt tcggccagtg
    52921 cttcactaac tgctagccac cactggcgag gagtcatttc agccgcagaa tttaagccat
    52981 aacgctgcca ctgacgtgaa agcgcttcct gaaattgctt atcgttaaaa ataggttgtg
    53041 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
    53101 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
    53161 aggaaatgtg atctcaacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa
    53221 tgcaatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
    53281 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcat aagtaaaata
    53341 tccagt
//
LOCUS       DAJSKJ010000007        53289 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000007 DAJSKJ010000000
VERSION     DAJSKJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.007"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            337..423
                     /gene="leuL"
                     /locus_tag="ON345_000352"
     CDS             337..423
                     /gene="leuL"
                     /locus_tag="ON345_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR3070465.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            516..2087
                     /gene="leuA"
                     /locus_tag="ON345_000353"
     CDS             516..2087
                     /gene="leuA"
                     /locus_tag="ON345_000353"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR3070466.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2087..3178
                     /gene="leuB"
                     /locus_tag="ON345_000354"
     CDS             2087..3178
                     /gene="leuB"
                     /locus_tag="ON345_000354"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR3070467.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3181..4581
                     /gene="leuC"
                     /locus_tag="ON345_000355"
     CDS             3181..4581
                     /gene="leuC"
                     /locus_tag="ON345_000355"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR3070468.1"
                     /translation="MANTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4592..5197
                     /gene="leuD"
                     /locus_tag="ON345_000356"
     CDS             4592..5197
                     /gene="leuD"
                     /locus_tag="ON345_000356"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR3070469.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5246..6424)
                     /gene="setA"
                     /locus_tag="ON345_000357"
     CDS             complement(5246..6424)
                     /gene="setA"
                     /locus_tag="ON345_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR3070470.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6526..6657)
                     /gene="sgrT"
                     /locus_tag="ON345_000358"
     CDS             complement(6526..6657)
                     /gene="sgrT"
                     /locus_tag="ON345_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR3070471.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6746..8401
                     /gene="sgrR"
                     /locus_tag="ON345_000359"
     CDS             6746..8401
                     /gene="sgrR"
                     /locus_tag="ON345_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR3070472.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANKQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8435..8534
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8565..9548
                     /gene="thiB"
                     /locus_tag="ON345_000360"
     CDS             8565..9548
                     /gene="thiB"
                     /locus_tag="ON345_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR3070473.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9524..11134
                     /gene="thiP"
                     /locus_tag="ON345_000361"
     CDS             9524..11134
                     /gene="thiP"
                     /locus_tag="ON345_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR3070474.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNCQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11118..11816
                     /gene="thiQ"
                     /locus_tag="ON345_000362"
     CDS             11118..11816
                     /gene="thiQ"
                     /locus_tag="ON345_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR3070475.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11930..12694)
                     /locus_tag="ON345_000363"
     CDS             complement(11930..12694)
                     /locus_tag="ON345_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR3070476.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12780..13658)
                     /gene="araC"
                     /locus_tag="ON345_000364"
     CDS             complement(12780..13658)
                     /gene="araC"
                     /locus_tag="ON345_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR3070477.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13997..15697
                     /gene="araB"
                     /locus_tag="ON345_000365"
     CDS             13997..15697
                     /gene="araB"
                     /locus_tag="ON345_000365"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR3070478.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15708..17210
                     /gene="araA"
                     /locus_tag="ON345_000366"
     CDS             15708..17210
                     /gene="araA"
                     /locus_tag="ON345_000366"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR3070479.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17410..18105
                     /gene="araD"
                     /locus_tag="ON345_000367"
     CDS             17410..18105
                     /gene="araD"
                     /locus_tag="ON345_000367"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3070480.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18180..20531
                     /gene="polB"
                     /locus_tag="ON345_000368"
     CDS             18180..20531
                     /gene="polB"
                     /locus_tag="ON345_000368"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR3070481.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGMIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20696..23602
                     /gene="rapA"
                     /locus_tag="ON345_000369"
     CDS             20696..23602
                     /gene="rapA"
                     /locus_tag="ON345_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR3070482.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23614..24273
                     /gene="rluA"
                     /locus_tag="ON345_000370"
     CDS             23614..24273
                     /gene="rluA"
                     /locus_tag="ON345_000370"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR3070483.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24390..25205)
                     /gene="djlA"
                     /locus_tag="ON345_000371"
     CDS             complement(24390..25205)
                     /gene="djlA"
                     /locus_tag="ON345_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR3070484.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25460..27814
                     /gene="lptD"
                     /locus_tag="ON345_000372"
     CDS             25460..27814
                     /gene="lptD"
                     /locus_tag="ON345_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR3070485.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVISRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27867..29153
                     /gene="surA"
                     /locus_tag="ON345_000373"
     CDS             27867..29153
                     /gene="surA"
                     /locus_tag="ON345_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR3070486.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29153..30142
                     /gene="pdxA"
                     /locus_tag="ON345_000374"
     CDS             29153..30142
                     /gene="pdxA"
                     /locus_tag="ON345_000374"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR3070487.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTVGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30139..30960
                     /gene="rsmA"
                     /locus_tag="ON345_000375"
     CDS             30139..30960
                     /gene="rsmA"
                     /locus_tag="ON345_000375"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR3070488.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30963..31340
                     /gene="apaG"
                     /locus_tag="ON345_000376"
     CDS             30963..31340
                     /gene="apaG"
                     /locus_tag="ON345_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR3070489.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31347..32189
                     /gene="apaH"
                     /locus_tag="ON345_000377"
     CDS             31347..32189
                     /gene="apaH"
                     /locus_tag="ON345_000377"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR3070490.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32267..32746)
                     /gene="folA"
                     /locus_tag="ON345_000378"
     CDS             complement(32267..32746)
                     /gene="folA"
                     /locus_tag="ON345_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR3070491.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32938..34800)
                     /gene="kefC"
                     /locus_tag="ON345_000379"
     CDS             complement(32938..34800)
                     /gene="kefC"
                     /locus_tag="ON345_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR3070492.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34793..35323)
                     /gene="kefF"
                     /locus_tag="ON345_000380"
     CDS             complement(34793..35323)
                     /gene="kefF"
                     /locus_tag="ON345_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR3070493.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35431..36762)
                     /locus_tag="ON345_000381"
     CDS             complement(35431..36762)
                     /locus_tag="ON345_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3070494.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36821..37108)
                     /gene="fixX"
                     /locus_tag="ON345_000382"
     CDS             complement(36821..37108)
                     /gene="fixX"
                     /locus_tag="ON345_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR3070495.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37105..38391)
                     /locus_tag="ON345_000383"
     CDS             complement(37105..38391)
                     /locus_tag="ON345_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3070496.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTMILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38442..39383)
                     /locus_tag="ON345_000384"
     CDS             complement(38442..39383)
                     /locus_tag="ON345_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR3070497.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39398..40168)
                     /locus_tag="ON345_000385"
     CDS             complement(39398..40168)
                     /locus_tag="ON345_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR3070498.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40642..42156
                     /gene="caiT"
                     /locus_tag="ON345_000386"
     CDS             40642..42156
                     /gene="caiT"
                     /locus_tag="ON345_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR3070499.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42187..43329
                     /gene="caiA"
                     /locus_tag="ON345_000387"
     CDS             42187..43329
                     /gene="caiA"
                     /locus_tag="ON345_000387"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR3070500.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43458..44675
                     /gene="caiB"
                     /locus_tag="ON345_000388"
     CDS             43458..44675
                     /gene="caiB"
                     /locus_tag="ON345_000388"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR3070501.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44749..46302
                     /gene="caiC"
                     /locus_tag="ON345_000389"
     CDS             44749..46302
                     /gene="caiC"
                     /locus_tag="ON345_000389"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR3070502.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLIDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNHPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46411..47195
                     /gene="caiD"
                     /locus_tag="ON345_000390"
                     /pseudo
     CDS             46411..47195
                     /gene="caiD"
                     /locus_tag="ON345_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
     gene            47201..47791
                     /gene="caiE"
                     /locus_tag="ON345_000391"
     CDS             47201..47791
                     /gene="caiE"
                     /locus_tag="ON345_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR3070503.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47877..48272)
                     /gene="caiF"
                     /locus_tag="ON345_000392"
     CDS             complement(47877..48272)
                     /gene="caiF"
                     /locus_tag="ON345_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR3070504.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48533..51754)
                     /gene="carB"
                     /locus_tag="ON345_000393"
     CDS             complement(48533..51754)
                     /gene="carB"
                     /locus_tag="ON345_000393"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR3070505.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51772..52920)
                     /gene="carA"
                     /locus_tag="ON345_000394"
     CDS             complement(51772..52920)
                     /gene="carA"
                     /locus_tag="ON345_000394"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR3070506.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        12788 a        14460 c        14000 g        12041 t
ORIGIN      
        1 attgatcaat aatattcacc aaatcaatat caaaaaaaat cgcaaaacat ataattcaat
       61 acaaatcatc aggataggtt ttgcaacgcg tgcattttgt cccctttttc ctcgttgatt
      121 agatgcaaaa atttatgctg aaatatgtca accgatgaaa agcgtcggta gttaagcaga
      181 aattaatatc gcttacttta accaccgcag cacaattagc taattttacg gatgcagaac
      241 tcacgctggc gggacgtttt tattgcgtca gggttgacat ccgtttttgt atccagtaac
      301 tctaaaagca tatcgcattc atctggagct gatttaatga ctcacatcgt tcgctttatc
      361 ggtctactac tactaaacgc atcttctttg cgcggtagac gagtgagcgg catccagcat
      421 taagccagca cgcagtcaaa caaaaaaccc gcgccattgc gcgggttttt ttatgcccga
      481 agcgaggcgc tctaaaagag acaaggaccc aaaccatgag ccagcaagtc attattttcg
      541 ataccacatt gcgcgacggt gaacaggcgt tacaggcaag cttgagtgtg aaagaaaaac
      601 tgcaaattgc gctggccctt gagcgtatgg gtgttgacgt gatggaagtc ggtttccccg
      661 tctcttcgcc gggcgatttt gaatcggtgc aaaccatcgc ccgccaggtt aaaaacagcc
      721 gcgtatgtgc gttagctcgc tgcgtggaaa aagatatcga cgtggcggcc gaatccctga
      781 aagtcgccga agccttccgt attcatacct ttattgctac ctccccaatg cacatcgcca
      841 ccaagctgcg cagcaccctg gacgaggtga tcgaacgcgc tatctatatg gtgaaacgcg
      901 cccgtaatta caccgatgat gttgaatttt cttgcgaaga tgccgggcgt acacccattg
      961 ccgatctggc gcgagtggtc gaagcggcga ttaatgccgg tgccaccacc atcaacattc
     1021 cggacaccgt gggctacacc atgccgtttg agttcgccgg aatcatcagc ggcctgtatg
     1081 aacgcgtacc taacatcgac aaagccatta tttccgtaca tacccacgac gatttaggcc
     1141 tggcggtcgg caactcactg gcggcggtac atgccggtgc acgccaggtg gaaggtgcaa
     1201 tgaacgggat cggcgagcgt gccggtaact gttcgctgga agaagtcatc atggcgatta
     1261 aagttcgtaa ggatattctc aacgtccaca ccgccattaa tcaccaggag atatggcgca
     1321 ccagccagtt agttagccag atttgtaata tgccgatccc ggcaaacaaa gccattgttg
     1381 gcagcggcgc attcgctcac tcctccggta tccaccagga tggcgtgctg aaaaaccgcg
     1441 aaaactacga aatcatgaca ccagaatcta ttggtctgaa ccaaatccag ctgaatctga
     1501 cctctcgttc ggggcgtgcg gcggtgaaac atcgcatgga tgagatgggg tataaagaaa
     1561 gtgaatataa tttagacaat ttgtacgatg ctttcctgaa gctggcggac aaaaaaggtc
     1621 aggtgtttga ttacgatctg gaggcgctgg ccttcatcgg taagcagcaa gaagagccgg
     1681 agcatttccg tctggattac ttcagcgtgc agtctggctc taacgatatc gccaccgccg
     1741 ccgtcaaact ggcctgtggc gaagaagtca aagcagaagc cgccaacggt aacggtccgg
     1801 tcgatgccgt ctatcaggca attaaccgca tcactgacta taacgtcgaa ctggtgaaat
     1861 acagcctgac cgccaaaggt cacggtaaag atgcgctggg tcaggtggat attgtcgcca
     1921 actacaacgg tcgccgcttc cacggcgtcg gcctggccac cgatattgtc gagtcctccg
     1981 ccaaagccat ggtgcacgta ctgaacaata tctggcgtgc cgcagaagtc gaaaaagagt
     2041 tgcaacgcaa agctcaacac aacgaaaaca acaaggaaac cgtgtgatgt cgaagaatta
     2101 ccatattgcc gtattgccgg gggacggtat tggtccggaa gtgatgaccc aggcgctgaa
     2161 agtgctgaat gccgtgcgca accgctttgc gatgcgcatc accaccagcc attacgatgt
     2221 aggcggcgca gccattgata accacgggca accactgccg cctgcgacgg ttgaaggttg
     2281 tgagcaagcc gatgccgtgc tgtttggctc ggtaggcggc ccgaagtggg aacatttacc
     2341 accagaccag caaccagaac gcggcgcgct gctgccgctg cgtaagcact tcaaattatt
     2401 cagcaacctg cgcccggcaa aactgtatca ggggctggaa gcattctgtc cgctgcgtgc
     2461 agacattgcc gctaacggct tcgacatcct gtgcgtgcgc gaactgaccg gcggcatcta
     2521 tttcggtcag ccaaaaggcc gcgaaggtag cggacaatat gaaaaagcgt ttgataccga
     2581 ggtgtatcac cgttttgaga tcgagcgtat tgcccgcatc gcgtttgaat ctgctcgtaa
     2641 acgtcgccac aaagtcacct caatcgacaa agctaacgtg ctgcaatcct ctattttatg
     2701 gcgtgaaatc gtcaacgaga tcgccacgga atacccggat gtcgaactgg cgcatatgta
     2761 catcgacaac gccaccatgc agctgattaa agatccatca cagtttgacg ttctgctgtg
     2821 ctccaacctg tttggcgaca ttctgtctga cgagtgcgca atgatcactg gctcgatggg
     2881 catgttgcct tccgccagcc tgaacgagca aggttttggt ctgtatgaac cggcaggcgg
     2941 ctcggcacca gatatcgcag gcaaaaacat cgccaacccg attgcacaaa tcctgtcgct
     3001 ggcactgctg ctgcgttaca gcctggatgc cgatgatgcg gcttccgcca ttgaacgcgc
     3061 cattaaccgc gcattagaag aaggcattcg caccggggat ttagcccgtg gcgctgccgc
     3121 cgttagtacc gatgaaatgg gcgatatcat tgcccgctat gtggcagaag gggtgtaatc
     3181 atggctaata cgttatacga aaaattattc gacgctcacg tagtgtacga agccgaaaac
     3241 gaaaccccgc tgttatatat cgaccgccac ctagtgcatg aagtgacctc accgcaggcg
     3301 ttcgatggtc tgcgcgccca cggtcgcccg gtacgtcagc cgggcaaaac cttcgccacc
     3361 atggatcaca acgtctctac ccagaccaaa gacattaatg cctgtggtga aatggcgcgc
     3421 atccagatgc aggagctgat taaaaactgc aaagaatttg gcgtcgagct gtatgacctg
     3481 aatcacccgt atcaggggat cgtccacgta atggggccgg aacagggcgt gaccttgccg
     3541 gggatgacca ttgtctgcgg cgactcgcat accgccaccc acggcgcgtt tggcgcactg
     3601 gcctttggta tcggcacttc cgaagttgaa cacgtactgg caacgcaaac cctgaaacag
     3661 ggtcgtgcga agaccatgaa aattgaagtc cagggcaaag ccgcgccggg cattaccgca
     3721 aaagatatcg tgctggcaat tatcggtaaa accggtagcg caggcggcac cgggcatgtg
     3781 gtggagtttt gcggcgaagc aatccgtgat ttaagcatgg aaggtcggat gaccctgtgc
     3841 aatatggcaa tcgaaatggg cgcgaaagcc ggtctggttg cgccggacga aaccaccttt
     3901 aactatgtca aaggccgtct gcatgcgccg aaaggcaaag atttcgacga cgccgttgcc
     3961 tactggaaaa ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt cactctgcaa
     4021 gcagaagaaa tttcaccgca ggtcacctgg ggcaccaatc caggccaggt gatttccgtg
     4081 aacgacaata ttcccgatcc ggcttcgttt gccgatccgg ttgaacgcgc gtcggcagaa
     4141 aaagcgctgg cctatatggg gctgaaaccg ggcattccgc tgaccgaagt ggctatcgac
     4201 aaagtgttta tcggttcctg taccaactcg cgcattgaag atttacgcgc ggcagcggag
     4261 atcgccaaag ggcgaaaagt cgcgccaggc gtgcaggcac tggtggttcc cggctctggc
     4321 ccggtaaaag cccaggcgga agcggaaggt ctggataaaa tctttattga agccggtttt
     4381 gaatggcgct tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg
     4441 ggcgaacgtt gtgcatccac cagcaaccgt aactttgaag gccgccaggg gcgcggcggg
     4501 cgcacgcatc tggtcagccc ggcaatggct gccgctgctg ctgtgaccgg acatttcgcc
     4561 gacattcgca acattaaata aggagcacac catggcagag aaatttatca aacacacagg
     4621 cctggtggtt ccgctggatg ccgccaatgt cgataccgat gcaatcatcc cgaaacagtt
     4681 tttgcagaaa gtgacccgta cgggttttgg cgcgcatctg tttaacgact ggcgttttct
     4741 cgatgaaaaa ggccaacagc caaacccgga cttcgtgctg aacttcccgc agtatcaggg
     4801 cgcttccatt ttgctggcac gagaaaactt cggctgtggc tcctcgcgtg agcacgcgcc
     4861 ctgggcattg accgactacg gttttaaagt ggtgattgcg ccgagttttg ctgacatctt
     4921 ctacggcaat agctttaaca accagctgct gccggtgaaa ttaagcgatg cagaagtgga
     4981 cgaactgttt gcgctggtga aagccaatcc ggggatccat ttcgacgtgg atctggaagc
     5041 gcaagaggtg aaagcgggag agaaaaccta tcgctttacc atcgatgcct tccgccgcca
     5101 ctgcatgatg aacggtctgg acagtattgg gcttaccttg cagcacgacg acgccattgc
     5161 cgcttatgaa gcaaaacaac ctgcgtttat gaattaatcc ccttgcccgg tcaaatgacc
     5221 gggctttccg ctatcgtcca cgtcatcaaa cgtctttaac ctttgcggtt aaaaataatg
     5281 cgacaacaga aataaccgca attacccagt agacagcaaa gtgcccccaa ctttgtgcaa
     5341 ttgctccctg aataacgcca gccagaatta ccccggtaga aatactgtta gtaaataagg
     5401 tggtagctgc ccccgctctt ccaggcatta aatcctgaaa ccatagcatc ccaatacccg
     5461 caacaatgcc gataaataca gcgttaaaaa gttgcagcgt catcaacgcc atacggctat
     5521 gaaagaaaat caatccggtg taaaacagta ctcctgccgc cactgctatg accatcattc
     5581 gccgcttacc ataacgtttg acatagtagc cagccagaat cattgctggt atttccagtc
     5641 cagctgccgt ccccatcagg aaacccgcca gtttgtctgg caatcctaac tcgctactga
     5701 tccacaacgg catatcaata atgtacatgg tgttgcaggt ccacattaac gtcgaggcga
     5761 caaataacat ccgtacgtta ctatcctgcc agccaccttg cattgataaa gcattttccg
     5821 acggcagttc tacccgcgcc acagacggaa gcataaatgc aatcaatacc agactgagtg
     5881 tgaatatccc ggcggcaatc gaaaacatta ccgtaaagcc gtaattcaac gccagcataa
     5941 aggccaacgg tggaccgata acccatgcca gagaaagctg cgcacgcatc accgagctaa
     6001 acatcaccac ttctcgcgcc gagttatccg catattcccg cgccagagca aataactgtg
     6061 gcattgccgt attggccaga gatgccagaa gcacaccaca ggtgataagc gtcagataat
     6121 gacgattaaa tgcaaacaat agcgcattgc cgatagccat caaacagcaa aatataatca
     6181 gttttcgccg atcgccctga ctgtcagaac gttttgccaa ccagaggctt acgccgatcc
     6241 cagcaatagc attcaccgta taaaagagtc cgatccagaa aggttgcgcg ccaacctcac
     6301 gactcagaaa taagctcaat gtaggagcct gtagcgcccc ggccaccccc atcataaaag
     6361 cgaccagcat aaatgccgcg taaacaccgt tcatacggcg agccatcgtc attatccaga
     6421 tcatacgttc cctttttagc gcggcgagaa taaaaaaaac cagcaggtat aatctgctgg
     6481 cgggtgattt tacaccaata ctcagtcaca catgatgcag gcaagtcaac tttcagaatt
     6541 gcggtcatcc cactgcatca gtccttcaag catggttaat cgttgtggga cgcttaacca
     6601 acgcaaccag cacaacttcg ctgtcgcggt aaaatagtgc tgataaaact gacgcatggg
     6661 acacctcctt gcttcatcgt taggaattat tgacttaata tagggaaaat aaaattgctg
     6721 tcttttgcgc aggagttccc cttttatgcc atctgctcgt ctgcaacaac agttcatccg
     6781 cctgtggcaa tgctgcgagg gtaaatcgca ggacacaacg ctcaacgaac tggcagcgtt
     6841 attgagctgc tcgcgtcgtc atatgcgtac cctgctcaac accatgcagg atcgcggctg
     6901 gctgacgtgg gaagcggaag tcgggcgcgg taaacgctcg cgtctgacat tcctctatac
     6961 cgggctggcg cttcagcaac agcgggcgga agacctgctg gagcaggatc gtatcgacca
     7021 actagtgcag ttggttggcg acaaagcgac tgtgcggcaa atgctggttt ctcatctggg
     7081 ccgcagcttc cgccaggggc ggcacatcct gcgcgtgctc tactatcgtc cgttgcgtaa
     7141 tctgctacct ggcagcgcat tgcgccgttc cgaaacccat attgcccggc aaatcttcag
     7201 ttcgctaacg cgcataaatg aggaaaatgg ggaactggaa gcagacatcg cccaccactg
     7261 gcagcaaata tcaccgcttc actggcgttt ctttttgcgt ccaggagtcc attttcatca
     7321 tggtcgtgaa ctggaaatgg acgacgtgat cgcctcttta aaacgaatca atacgctgcc
     7381 gctctattcg catattgctg acattgtgtc gccgacgccc tggacgctgg atatccatct
     7441 cacgcaaccg gaccgctggt taccgttatt gctggggcaa gttccggcga tgatcctgcc
     7501 gcgcgaatgg gaaaccctca gtaactttgc cagccatccc atcggcaccg gtccgtatgc
     7561 ggtgatccgc aacagcacca atcaactgaa aattcaggca ttcgatgact tcttcggtta
     7621 ccgggcatta atcgacgaag ttaacgtctg ggttctgccg gaaattgccg acgagccagc
     7681 cggaggtctg atgctaaaag gtccacaggg cgaggaaaaa gagattgaaa gccgcctgga
     7741 ggaaggttgc tactatttac tgttcgatag ccgcacccat cgcggggcga ataagcaagt
     7801 cagggactgg gtaagctatg tgctttctcc aactaatctg gtctatttcg ctgaggaaca
     7861 gtaccagcaa ctgtggttcc cggcttatgg actgctcccc cgttggcacc atgcccgcac
     7921 cataaagagc gaaaaaccgg ctggtctgga aagcctcacc ctgacctttt atcaggatca
     7981 cagtgagcat cgggtgattg ccgggatcat gcagcagatt ctggcaagtc accaggtcac
     8041 gctggaaatc aaagagatca gctacgatca gtggcatgaa ggagagatcg agagcgatat
     8101 ctggcttaat agcgccaact ttacgctgcc gctggatttt tcgctgttcg cgcacttgtg
     8161 cgaggtgccg ctgctccaac actgtattcc gatcgactgg caagccgacg ccgcccgctg
     8221 gcgcaatggc gaaatgaacc tggcgaactg gtgccagcaa ctggtcgcca gtaaagcaat
     8281 ggtgccgctt atccaccact ggctgatcat tcagggacaa cgcagtatgc gcggcctgcg
     8341 tatgaatacc ctcggctggt tcgattttaa atcagcgtgg tttgcgccgc cggatccatg
     8401 attgctggta gataacaaaa tcactacact aacgccgttc tcaacggggt gccacgcaaa
     8461 cgcgtgcgct gagaaaatac ccgtcgaacc tgatccggat aacgccggcg aagggatttg
     8521 aggctccttc tcaagtcctt tgccactctt ttttgaggtg caaagtgtta aaaaaatgtc
     8581 ttcccctgct gttgctgtgc acagcgcccg ttttcgctaa acccgttctg actgtttata
     8641 cctacgattc cttcgccgcc gactgggggc ctggtccaaa gatcaaaaaa gcttttgaag
     8701 ccgactgtaa ctgcgaactg aaactggtgg cgctggaaga tggcgtttcg cttctcaacc
     8761 gtctacggat ggaaggcaaa aatagtaaag ccgatgtggt gctggggctg gataacaatc
     8821 tgttagacgc cgccagtaaa accggactat ttgccaaaag cggtgtggca gcggatgccg
     8881 ttaacgttcc cggcggctgg aataatgaca ctttcgtccc gtttgattac ggctatttcg
     8941 ccttcgtcta tgacaagaac aaactgaaaa acccgccgca aagcctgaaa gaattggttg
     9001 agagcgatca aaactggcgg gtgatttatc aggatccgcg caccagtacg ccggggctgg
     9061 gcctgctgtt gtggatgcaa aaagtctatg gcgatgacgc cccgcaggcc tggcaaaaac
     9121 tggcgaagaa aaccgtcacg gtcaccaaag gctggagcga agcctacggc ctgtttttaa
     9181 aaggtgaaag cgatctggta ctgagttaca ccacctctcc ggcttatcac attctcgaag
     9241 agaaaaaaga taactacgcc gccgcgaact ttagcgaagg tcactatctg caggtggaag
     9301 tcgccgctcg caccgctgcc agcaagcagc cggagctggc gcaaaaattc ctccagttta
     9361 tggtttctcc ggcttttcag aatgcgatcc caaccggtaa ctggatgtat ccggtggcaa
     9421 atgtcacgct gcctgccggg tttgaacaat tgaccaaacc ggccaccacg ctggagttca
     9481 caccagctga agtggcggca caacgtcagg catggattag cgaatggcaa cgcgccgtca
     9541 gccgttaatt cccggctggt taattccagg tgtaagcgcc gccacgctgg tggtagcggt
     9601 tgcgctcgcg gcgtttctcg ccctgtggtg gaacgcgccg cagggtaact gggtggcagt
     9661 ctggcaggac agctacctgt ggcatgtggt gcgcttctcc ttctggcagg cgtttctctc
     9721 ggcgctactc tctgtcgtac ccgcgatatt cctcgcccgc gccctctatc gcaggcgctt
     9781 tccgggtcgg ctggcgctgt tgcgtctgtg cgcaatgacc ttgatcctcc cggtgctggt
     9841 tgctgttttc ggcattctta gcgtctatgg tcgccagggc tggctggcat cgctctgcca
     9901 atcgctcggt ctggagtgga ccttttcgcc ctacggcctg caaggtattt tgctggcgca
     9961 cgtatttttt aatctgccga tggcgagccg cttattactc caggcactgg aaaacattcc
    10021 cggcgaacaa cgtcagcttg ccgcccagct tgggatgcgc ggctggcatt ttttccgctt
    10081 cgtcgaatgg ccgtggttac ggcgacaaat cccgccggtt gccgcgctta tctttatgct
    10141 ctgtttcgcc agcttcgcca ccgtgctatc gctgggcggc ggtccgcagg caactactat
    10201 cgagctggct atttatcagg cgctgagtta cgactacgat cctgcccgcg cggcgatgct
    10261 ggcgctgctc cagatggtgt gttgccttgg gctggtgctg ttgagtcagc gactaagtaa
    10321 ggccattgcg cccggtacca cgctgctgca aggctggcgc gatccggacg atcgtctgca
    10381 tagccgcatt tgcgacacgg tgttaatagt gctggcgctg ctgctgttgc tgccaccgtt
    10441 gctggcggtg atcgtcgatg gggtaaattg ccagttgccg gaagtgctgg cacaaccggt
    10501 gctgtggcag gcgctgtgga cctcgttgcg tattgcgctg gcggcaggtg tattgtgcgt
    10561 agtgctgacc atgatgctgc tatggagcag tcgcgaactg cgggcgcggc agaaaatgct
    10621 ggcgggtcag gcgctggaga tgagcggcat gttgatcctc gccatgccgg ggattgtgct
    10681 ggctaccggc ttctttttac tgctcaacaa cactatcggc ctgccacaat ctgctgacgg
    10741 cattgtgatt ttcaccaatg cgttaatggc gatcccttat gcgctgaaag tactggaaaa
    10801 cccgatgcgc gatatcaccg cccgctacag tatgttgtgt cagtcgctgg gcattgaagg
    10861 ctggtcgcgc ttaaaagtgg tcgagctgcg cgccctgaaa cgtccactgg cgcaggcgct
    10921 ggcttttgcc tgcgtgctgt cgattggtga ttttggcgta gtggcgttgt tcggtaacga
    10981 tgatttccgt accctgccgt tttatctcta ccagcaaatt ggctcctatc gcagccagga
    11041 cggcgcggtc accgcgttaa ttctgctact actctgtttt ctgctgttta ccgtgattga
    11101 aaaactaccg gggcgaaatg ttaaaactga ctgatatcac ctggctttat caccatttgc
    11161 cgatgcgttt tagcttaacg gtagaacgcg gcgagcaggt ggcaatcctc gggccaagcg
    11221 gcgcgggtaa aagtaccctg ctgaatttga ttgccggttt tctgacgcca gccagcggtt
    11281 cgctgactat cgacagcgtt gatcacacca cgacgccgcc gtcacgccgt ccggtatcga
    11341 tgctgtttca ggagaacaac ctgttcagcc acctgacggt cgcgcagaac atcgggctgg
    11401 ggcttaatcc gggattgaag ctgaacgcgg cgcagcagga gaaaatgcac gctatcgccc
    11461 gccagatggg gattgataat ttaatggcgc ggttaccggg cgagctttcc ggcggtcagc
    11521 gacagcgcgt ggcgttagcg cgttgtctgg tgcgcgaaca gccgatttta ttgctcgatg
    11581 aaccgttctc tgcgctcgat ccggcgttac gtcaggagat gctgacgctg gtgagcacga
    11641 gctgccagca gcaaaaaatg acgctattga tggtgtcgca cagtgtggaa gatgcggcgc
    11701 ggatcgccac acgctcggtg gtggtcgccg acgggcgcat cgcctggcag ggtaagactg
    11761 aagaattgtt gagcggtaag gcgagtgctt cggcaatatt ggggattacg ggttagtatg
    11821 cactgtcaga tgcggcgtga acaccttatc cgacctacgg acagtgcgtt ttgtaggcct
    11881 gataagacgc ggtcagcgtc gcatcaggca acccgcacaa gacaatcccc taaaccccaa
    11941 ccactttacg caaaatatcg atatacaccg gcatcaacgg gtggcgaatt aacaccacca
    12001 gcgccaccac gccgatggca gaaatcaacg gcgtcagcca caacaaacgg ccgcgcgaca
    12061 aataatgact taaacgatcg gttgctttac cgctgcgcca taaccgccag cacagccagc
    12121 caccaaccca caaaaatacc gccgtcgcca gcagcaacca tttaaactcg ccgctctgca
    12181 ttccggctgg aatatcgatc gccgcgcccg ccagaatccc aggcaggaag taaaacggtg
    12241 gccacagcag gcagccgata atattcggcg taatgaattt tgccaccggc agatccagca
    12301 ttcccgccac cattggcacc agcggacgcg tcgggccaac aaagcgaccg accagaatgg
    12361 tgaacatgct gtgttgatgc aacgcatgtt cagttttatc gagtaatgct ttgtttttct
    12421 tcagaaatga ccagcgatgc aacggctttt taaaacgcca gcccagccag aaggagatcc
    12481 agtcgcccag cagacaaccg acaatccctg ccagccaggc gtgccagaaa cttaactcgc
    12541 cgctgccaat cagcgctccc agccccgcca tcagcaccgt accgggtaga atcaaaccga
    12601 ccagcgccag cgactccaga aaggccacca acaccaccgc catcaatgaa tacacggtag
    12661 attgggtaat aaagtgttcc agcaatgctt gcataatgtg cctgtcaaat ggacgaagca
    12721 gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat
    12781 tatgacaact tgacggctac atcattcact ttttcttcac aaccggctcg gaactcgctc
    12841 gggctggccc cggtgcattt tttaaatact cgcgagaaat agagttgatc gtcaaaacca
    12901 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctga
    12961 ctaatgcgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
    13021 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
    13081 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
    13141 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
    13201 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
    13261 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaacccgt attggcaaat
    13321 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
    13381 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
    13441 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
    13501 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
    13561 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
    13621 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
    13681 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
    13741 ctcttctcgc taacccaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
    13801 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
    13861 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
    13921 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
    13981 tggatggagt gaaacgatgg cgattgcaat tggcctcgat tttggcagtg attctgtgcg
    14041 agctttggcg gtggactgcg ccagcggtga agagatcgcc accagcgtag agtggtatcc
    14101 ccgttggcaa aaagggcaat tttgtgatgc cccgaataac cagttccgtc atcatccgcg
    14161 tgactacatt gagtcaatgg aagcggcact gaaaaccgtg cttgcagagc ttagcgtcga
    14221 acagcgcgca gctgtggtcg ggattggcgt tgacagtacc ggctcgacgc ccgcaccgat
    14281 tgatgccgac ggtaacgtgc tggcgctgcg cccggagttt gccgaaaacc caaacgcgat
    14341 gttcgtattg tggaaagacc acactgcggt tgaagaagcg gaagagatta cccgtttgtg
    14401 ccacgcgccg ggcaacgttg actactcccg ctacattggt ggtatttatt ccagcgaatg
    14461 gttctgggca aaaatcctgc atgtgactcg ccaggacagc gccgtggcgc aatctgccgc
    14521 atcgtggatt gagctgtgcg actgggtgcc agctctgctt tccggtacca cccgcccgca
    14581 ggatattcgt cgcggacgtt gcagcgccgg gcataagtcg ctatggcacg aaagctgggg
    14641 tggcttgccg ccagccagtt tctttgatga gctggacccg atcctcaatc gccatttgcc
    14701 ttccccgctg ttcactgaca cctggactgc cgatattccg gtgggcacct tatgcccgga
    14761 atgggcgcag cgtctcggcc tgcctgaaag cgtggtgatt tccggcggcg cgtttgactg
    14821 ccatatgggc gcagttggcg caggcgcaca gcctaacgca ctggtaaaag ttatcggtac
    14881 ttccacctgc gacattctga ttgccgacaa acagagcgtt ggcgagcggg cagttaaagg
    14941 tatttgcggt caggttgatg gcagcgtggt gcctggattt atcggtctgg aagcaggcca
    15001 atcggcgttt ggtgatatct acgcctggtt tggtcgcgta ctcggctggc cgctggaaca
    15061 gcttgccgcc cagcatccgg aactgaaagc gcaaatcaac gccagccaga aacaactgct
    15121 tccggcgctg accgaagcat gggccaaaaa tccgtctctg gatcacctgc cggtggtgct
    15181 cgactggttt aacggtcgtc gcacaccgaa cgctaaccaa cgcctgaaag gggtgattac
    15241 cgatcttaac ctcgctaccg acgctccgct gctgttcggc ggtttgattg ctgccaccgc
    15301 ctttggcgca cgcgcaatca tggagtgctt taccgatcag gggatcgccg tcaataacgt
    15361 gatggcgctg ggcggcatcg cgcggaaaaa ccaggtcatt atgcaggcct gctgcgacgt
    15421 gctgaatcgc ccgctgcaaa ttgttgcctc tgaccagtgc tgtgcgctcg gtgcggcgat
    15481 ttttgctgcc gtcgccgcga aagtgcacgc agacatccca tcagcccagc aaaaaatggc
    15541 cagtgcggta gagaaaaccc tgcaaccgcg cagcgaacag gcacaacgct ttgaacagct
    15601 ttatcgccgc tatcagcaat gggcgatgag cgccgaacaa cactatcttc caacttccgc
    15661 cccggcacag gctgcccagg ccgttgcgac tctataagga cacgataatg acgatttttg
    15721 ataattatga agtatggttt gtcattggca gccagcatct gtatggcccg gaaaccctgc
    15781 gtcaggtcac ccaacatgcc gagcacgttg ttaatgcgct gaatacggaa gcgaaactgc
    15841 cctgcaaact ggtgttgaaa ccgctgggca ccacgccgga tgaaatcacc gctatttgcc
    15901 gcgacgcgaa ttacgacgat cgttgcgctg gtctggtggt gtggctgcac accttctccc
    15961 cggccaaaat gtggatcaac ggcctgacca tgctcaacaa accgttgctg caattccaca
    16021 cccagtttaa cgcggcgctg ccgtgggaca gtatcgatat ggactttatg aacctgaacc
    16081 agactgcaca tggcggtcgc gagttcggct tcattggcgc gcgtatgcgt cagcaacatg
    16141 ccgtggttac cggtcactgg caggataaac aagcccatga gcgtatcggc tcctggatgc
    16201 gccaggcggt ctctaaacag gatacccgtc atctgaaagt ctgccgtttt ggcgataaca
    16261 tgcgtgaagt agcggtcacc gatggtgata aagttgccgc acagatcaag ttcggtttct
    16321 ccgtcaatac ctgggcggtt ggcgatctgg tgcaggtggt gaactccatc agcgacggcg
    16381 atgttaacgc gctggtcgat gagtacgaaa gctgctacac catgacgcct gccacacaaa
    16441 tccacggcga aaaacgacag aacgtgctgg aagcggcgcg tattgagctg gggatgaagc
    16501 gtttcctgga acaaggtggc ttccacgcgt tcaccaccac ctttgaagat ttgcacggtc
    16561 tgaaacagct tccaggtctg gccgtacagc gtctgatgca gcagggttac ggctttgcgg
    16621 gcgaaggcga ctggaaaacc gccgccctgc ttcgcatcat gaaggtgatg tcaaccggtc
    16681 tgcagggcgg cacctccttt atggaggact acacctatca cttcgagaaa ggtaatgacc
    16741 tggtgctcgg ctcccatatg ctggaagtct gcccgtcgat tgccgtagaa gagaaaccga
    16801 tcctcgacgt tcagcatctc ggtattggtg gtaaggacga tcctgcccga ctgatcttca
    16861 atacccaaac cggtccagcg attgtcgcca gcctgattga tctcggcgat cgttaccgtc
    16921 tgctggttaa ctgtatcgac acggtgaaaa caccgcactc cctgccgaaa ctgccggtgg
    16981 cgaatgcgct gtggaaagcg caaccggatc tgccaactgc ttccgaagcg tggatcctcg
    17041 ctggtggcgc gcaccatacc gtcttcagcc atgcgctgaa cctcaacgat atgcgccagt
    17101 tcgccgagat gcacgacatt gaaatcacgg tgattgataa cgacacccgc ctgccagcgt
    17161 ttaaagacgc actgcgctgg aacgaagtgt attacggatt tcgtcgctaa gtagtcacat
    17221 caggtatgta acgcctgatg cgacgctgac gcgtcttatc tggcctacac gctgcgattt
    17281 tgtaggccgg ataagcaaag cgcatccggc attcaacgcc tgatgcgacg ctggcgcgtc
    17341 ttatcaggcc tacgcgctgc gattttgtag accggataag cgaagcgcat ccggcacgaa
    17401 ggagtcaaca tgttagaaga tctcaaacgc caggtattag aagccaacct ggcgctgcca
    17461 aaacacaacc tggtcacgct cacatggggc aacgtcagcg ccgttgatcg cgagcgcggc
    17521 gtctttgtga tcaaaccttc cggcgtcgat tacagcgtca tgaccgctga cgatatggtc
    17581 gtggttagca tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc ctccgacacg
    17641 ccaactcacc ggctgctcta tcaggcattc ccctccattg gcggcattgt gcatacgcac
    17701 tcgcgccacg ccaccatctg ggcgcaggcg ggtcagtcga ttccagcaac cggcaccacc
    17761 cacgccgact atttctacgg caccattccc tgcacccgca aaatgaccga cgcagaaatc
    17821 aacggcgaat atgagtggga aaccggtaac gtcatcgtag aaacctttga aaaacagggt
    17881 atcgatgcag cgcaaatgcc cggcgttctg gtccattccc acggcccgtt tgcatggggc
    17941 aaaaatgccg aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg
    18001 atattctgcc gtcagttagc gccgcagtta ccggatatgc agcaaacgct gctggataaa
    18061 cactatctgc gtaagcatgg cgcgaaggca tattacgggc agtaatgact gtataaaacc
    18121 acagccaatc aaacgaaacc aggctatact caagcctggt tttttgatgg attttcagcg
    18181 tggcgcaggc aggttttatc ttaacccgac actggcggga caccccgcaa gggacagaag
    18241 tctccttctg gctggcgacg gacaacgggc cgttgcaggt tacgcttgca ccgcaagagt
    18301 ccgtggcgtt tattcccgcc gatcaggttc cccgcgctca gcatattttg cagggtgaac
    18361 aaggctttcg cctgacaccg ctggcgttaa aggattttca ccgccagccg gtgtatggcc
    18421 tttactgtcg cgcccatcgc caattgatga attacgaaaa gcgcctgcgt gaaggtggcg
    18481 ttaccgtcta cgaggccgat gtgcgcccgc cagaacgcta tctgatggag cggtttatca
    18541 cctcaccggt gtgggtcgag ggtgatatgc acaatggcac tatcgttaat gcccgtctga
    18601 aaccgcatcc cgactatcgt ccgccgctca agtgggtttc tatagatatt gaaaccaccc
    18661 gccacggtga gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc gtttatatgc
    18721 tggggccgga gaatggcgac gcctcctcgc ttgatttcga actggaatac gtcgccagcc
    18781 gcccgcagtt gctggaaaaa ctcaacgcct ggtttgccaa ctacgatcct gatgtgatca
    18841 tcggttggaa cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc gagcgttacc
    18901 gtcttccgct gcgtcttggg cgcgataata gcgagctgga gtggcgcgag cacggcttta
    18961 aaaacggcgt cttttttgcc caggctaaag gtcggctaat tatcgacggt atcgaggcgc
    19021 tgaaatccgc gttctggaat ttctcttcat tctcgctgga aactgtcgct caggagctat
    19081 taggcgaagg aaaatctatc gataacccgt gggatcggat ggacgaaatt gaccgccgtt
    19141 tcgccgaaga taaacctgcg ctggcgactt ataacctgaa agattgcgag ctggtgacgc
    19201 agattttcca taaaactgaa atcatgccat ttttactcga acgggcaacg gtgaacggcc
    19261 tgccggtgga ccgacacggc ggttcggtgg cggcatttgg tcatctctat tttccgcgaa
    19321 tgcatcgcgc tggttatgtc gcgcctaatc tcggcgaagt gccgccgcac gccagccctg
    19381 gcggctacgt gatggattca cggcctgggc tttatgattc agtgctggtg ctggactata
    19441 aaagcctgta cccgtcgatc atccgcacct ttctgattga tcccgtcggg ctggtggaag
    19501 gcatggcgca gcctgatcca gagcacagta ccgaaggttt tctcgatgcc tggttctcac
    19561 gggagaaaca ttgcctgccg gagattgtga ctaacatctg gcacgggcgc gatgaagcca
    19621 aacgccaggg taataagccg ctttcgcagg cgctgaagat cattatgaat gccttttatg
    19681 gcgtgctcgg caccaccgcc tgccgcttct tcgatccgcg cctggcatcg tcgatcacca
    19741 tgcgtggtca tcagatcatg cggcaaacca aagcgttgat tgaagcacag ggctacgacg
    19801 ttatctacgg agataccgac tcgacttttg tctggctgaa aggtgcacat tcggaagaag
    19861 aggcggcgaa aatcggtcgt gcactggtgc agcacgttaa cgcctggtgg gcggaaacgc
    19921 tgcaaaaaca acggctgacc agcgcattag aactggagta tgaaactcat ttctgccgtt
    19981 ttcttatgcc aaccattcgc ggagctgata ccggcagtaa aaagcgctac gccgggatga
    20041 ttcaggaggg cgataaacag cggatggtgt ttaaagggct ggaaaccgtg cgcaccgact
    20101 ggacgccgct ggcccagcag tttcagcagg agctatacct gcgcatcttc cgcaacgagc
    20161 catatcagga gtacgtccgc caaaccatcg acaaactgat ggcgggtgaa ctggatgcgc
    20221 gactggttta ccgtaaacgc cttcgccgtc cgctgagcga gtatcagcgt aatgtgccgc
    20281 ctcatgtacg cgccgctcgc cttgccgatg aagaaaacca aaagcgtggt cgccccttgc
    20341 aatatcagaa tcgcggcacc attaagtacg tatggaccac caacggcccg gagccgctgg
    20401 actaccaacg ttcaccactg gattacgaac actatctgac ccgccagcta caacccgtgg
    20461 cggagggaat actccctttt attgaggata attttgctac acttatgacc gggcaacttg
    20521 ggctattttg agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg acgaatgctt
    20581 tgccatccag taccatagcg ccctttccat tcctggacct gaataacacc actacctcat
    20641 aagcacggta gcgggtggtt attgcctgca attaaagata tagagccgaa cacatatgcc
    20701 ttttacactt ggtcaacgct ggatcagcga tacagaaagc gaattgggac ttggaaccgt
    20761 tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca tctactggtg aaaaccgtct
    20821 gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc aaccctggtg ataccattac
    20881 cagccatgac ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac
    20941 ctatatcggt actcgcctgg atactgaaga gtccggcgta gccctgcgtg aagttttcct
    21001 tgatagcaaa ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc agattgaccg
    21061 tatggaccgc tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac agttccgtat
    21121 gccgtacagc ggcctgcgcg gtcagcgtac cagcctgatt ccgcatcagc tcaacatcgc
    21181 tcatgatgtt ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg
    21241 gaaaaccatt gaagccggga tgatcctgca tcagcaactg ctctctggcg ctgctgaacg
    21301 tgtgctgatt atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg
    21361 tttcaacctg cgctttgcac tgtttgatga tgagcgttat gccgaagctc agcacgatgc
    21421 ttacaacccg ttcgataccg aacagctggt gatttgctcg ctggattttg cccgtcgtag
    21481 caaacagcgt ctggaacatc tctgtgaagc cgagtgggac ctgctggtgg tcgatgaagc
    21541 gcatcacctg gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca ttgaacaact
    21601 ggcagagcac gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc tggggatgga
    21661 aagccacttc gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt
    21721 cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc tgctggcagg
    21781 taacaaactg agcaatgacg aactgaacat gctcggcgag atgatcggcg agcaggatat
    21841 cgagccgctg ttgcaggcag caaacagcga cagcgaagat gcccagagcg cccgtcagga
    21901 gctggtttcg atgctgatgg atcgccacgg caccagccgc gtgctgttcc gtaacacgcg
    21961 taacggtgtg aaaggattcc cgaaacgcga gctgcacacc attaaactgc cgctaccgac
    22021 gcagtatcag acggctatta aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga
    22081 tcgtgctcgc gatatgctct acccggagcg tatttatcag gaatttgaag gtgataacgc
    22141 cacctggtgg aacttcgatc cgcgcgttga gtggctgatg ggctacctga ccagccatcg
    22201 ctctcagaaa gtgctggtga tctgcgccaa agctgccact gcgctgcaac tggagcaagt
    22261 actgcgcgaa cgtgaaggta ttcgcgctgc ggtgttccac gaaggtatgt cgattatcga
    22321 acgtgaccgc gctgccgcct ggtttgccga agaagacacc ggcgcacagg tactgctgtg
    22381 ctcagaaatc ggttctgaag gacgtaactt ccagttcgcc agccacatgg tgatgtttga
    22441 cctgccattc aacccggatc tactggagca gcgtattggt cgtctggatc gtatcggcca
    22501 ggcgcacgat attcagatcc atgtgcctta tctggagaaa accgctcagt cggtgctggt
    22561 gcgctggtat cacgaaggtc tggatgcatt tgagcacacc tgcccgaccg gacgcactat
    22621 ttacgatagc gtatacaacg atctgattaa ctatctggct tcaccggatc aaaccgaagg
    22681 ctttgacgat ctgatcaaaa actgccgcga gcaacatgaa gcgctgaaag cacaactgga
    22741 acagggtcgt gaccgcctgc tggaaatcca ctccaacggt ggcgaaaaag cccaggcact
    22801 ggcagaaagc attgaagagc aggatgacga taccaacctg atcgccttcg ccatgaacct
    22861 gttcgatatt atcggtatca atcaggacga tcgcggcgac aacatgatcg tgctgacgcc
    22921 gtccgatcat atgctggtgc cggacttccc tggcctgtcg gaagatggca tcaccatcac
    22981 ctttgatcgt gaagtggcgc tggcgcgtga agatgcacag tttattacct gggagcatcc
    23041 gctgatccgc aacggtctgg atctgatcct ttctggcgat accggtagca gcacgatttc
    23101 actgttaaaa aacaaagcgt tgccggtagg tacgctgttg gtggaactga tttatgtggt
    23161 tgaagcccag gctccgaagc agttgcagct caaccgcttc ctgccaccga cgccggtacg
    23221 tatgctgctg gataaaaacg gcaacaacct ggcggcgcag gtagagtttg aaacctttaa
    23281 ccgccagctt aacgcggtta accgtcacac cggcagcaaa ctggttaacg ccgtgcagca
    23341 ggatgttcac gctatccttc aactgggtga agcgcagatc gagaaatctg cccgtgcatt
    23401 gattgatgca gcgcgtaacg aagccgacga aaaactgtct gccgagctgt ctcgtctgga
    23461 agctctgcgt gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa
    23521 ccgtcagcag gtaatggaaa gcctggatca ggcaggttgg cgtctggatg ccctgcgttt
    23581 gatcgttgta acgcatcagt aacggagccg aaaatgggga tggaaaacta caatccaccg
    23641 caggaaccct ggttggttat cctgtatcag gatgaccata ttatggtggt caacaagccg
    23701 agcggtttgt tgtcggtgcc gggtcgtctg gaagagcaca aagacagcgt gatgacgcgc
    23761 attcagcgtg attatccgca ggcagaatcg gtgcatcgtc tggatatggc taccagcggc
    23821 gtgattgtag tggcgctgac caaagccgcg gagcgggagt taaaacgcca gttccgcgag
    23881 cgcgagccga aaaagcagta tgtagcccgc gtctgggggc atccatcccc tgcggaaggt
    23941 ctggtggatc tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac
    24001 gaaacgggta aacctgcgca gacggaatat gaagtggtgg agtatgcggc ggataacacg
    24061 gcaagagtgg tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt gcatatgctg
    24121 gcgctggggc atccgattct cggcgatcgt ttttatgcat caccagaagc gagagcgatg
    24181 gcaccgcgtt tgttgctaca tgcagagatg ttgacgatta cccatccggc gtatggcaat
    24241 agtatgacgt ttaaagcgcc agcggatttt taatcgtcac gctggcgcag ttcggtaggc
    24301 ggataaggcg ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg cgacgcgggc
    24361 cgcgtcttat caggcctaca gtttaaaact tatttaaacc ctttctgctg ctttatcagc
    24421 tcatatgcct gctgaatttc ctgcgctttc tgcttcgcca tctccatcat ctcaggcggc
    24481 aaacctttcg ccaccagctt atcgggatgg tgttcactca tcagcttacg gtaggcacgt
    24541 ttgatggtgg tcgcatcatc cgtcggcttc acgcccagca cattacaggc atcttccagc
    24601 gtcgggccac gctgcgcttg ctgccagtta ccaccgccag attgctgctg ataaccgccg
    24661 ccaaactgtg caccgccctg catcatgcgc aaaaactggt caaactgggc gcgggagatc
    24721 cctaattctt cagcaatgac atacagcacc gcccgttcat tcgggtgcag tgaaccatca
    24781 gcaaacgccg cctgaatctg gatctccaga aacatacgaa ttaagtcaaa acgaccaaag
    24841 cagacactgc gaaactggcg catcttttcg cgcagcgggt aattgtctga ttttcccacc
    24901 cggaacgcgt tttgcgccgc agtacgggaa gcgccatgaa ggttcattcg gtccatcagc
    24961 tggctggcga tatgaatatc agcctccgtg acgcgacctt tggatttggt taaatgcccc
    25021 atcacttcaa aagtggtggc aaaaaacagc gcctgacgct cacgctggtt ggcgaaccac
    25081 gccattttac ggctacgggc tttatcaaac atatggccaa ttaacaggcc taacactacg
    25141 ccccaaaagc cgccgcccat cagtaaggcc acggccacgc caatgatttt tccccaatac
    25201 tgcatatatt ccccaaatcg acacacggat atcagggcta tctcccacaa tataaaggtg
    25261 cttttaccgt tttccggctg cggtcaattg tgggacatcg cctataattt gcattatcat
    25321 acctgtcatt caatgccgtg cctaacacca ctgacgctat tcggacagga ttaacactag
    25381 cgtagagatg acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt taccgatgat
    25441 ggaacaataa aatcaacgta tgaaaaaacg tatccccact ctcctggcca ccatgattgc
    25501 caccgccctt tatagtcaac agggactggc agccgacctc gcctcacagt gcatgttggg
    25561 cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc aatgacttac ccgtgactat
    25621 caatgctgac cacgcgaaag gggactaccc ggatgacgcc gtgtttactg gcagcgtgga
    25681 tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc
    25741 accaggacaa ccggagccgg tacgaaccgt tgatgcgctc ggtaatgtcc attacgacga
    25801 taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca aagataccaa
    25861 cgtctgggaa ggtgattacc agatggtggg tcgccagggc cgcggtaaag cggacctgat
    25921 gaaacaacgt ggtgaaaacc gctataccat tctggataac ggtagcttta cctcctgtct
    25981 gccgggttct gacacctgga gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca
    26041 agttgcggag atctggaacg cccgctttaa ggtgggtccg gtaccgatct tttatagccc
    26101 ctatttgcag ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa
    26161 gtacaccacc accaactact ttgagttcta cctgccatat tactggaaca tcgcgccaaa
    26221 tatggatgcc accatcacgc cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa
    26281 cgaattccgc tacctctccc aggcgggcgc tggcttgatg gaactggact atctgccttc
    26341 agataaagtc tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt ggttattcta
    26401 ctggaaccac tccggggtca tggatcaagt gtggcgtttc aacgtcgact acaccaaggt
    26461 cagcgatcct agctacttca atgatttcga taacaagtac ggttccagta ctgacggcta
    26521 cgcaacgcaa aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac
    26581 caagcagttc caggttttca gcgaacagaa caccagtagc tactcggcag agccgcagtt
    26641 agacgttaat tactaccaga atgatgttgg tccgtttgat acgcgtattt acggccaggc
    26701 agtgcacttt gttaacacca gagacgacat gcctgaagca acccgtgttc acctggaacc
    26761 gaccatcaat ttgccgctct ctaataactg gggcagcatc aataccgaag cgaagttgct
    26821 ggcaacccat tatcagcaaa ccaatcttga ctggtataac tccagaaaca cgaccaagct
    26881 ggacgaatcc gttaaccgcg taatgccgca attcaaagtt gacggcaaaa tggtctttga
    26941 acgcgatatg gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta
    27001 tttgtacgtg ccgtatcgcg atcagagcga catctataac tacgactcgt ctctgctgca
    27061 atctgactac tctggcctgt tccgggaccg gacttacggc ggtcttgacc gtattgcctc
    27121 cgctaaccag gtgacgaccg gtgtcatatc tcgcatatat gatgatgctg ccgttgaacg
    27181 ttttaatatt tccgttggtc aaatctacta tttcacggag tctcgcactg gcgatgacaa
    27241 cataacatgg gagaatgacg acaaaacggg ttcactggtg tgggcaggcg atacttactg
    27301 gcgtatctcc gagcgttggg gattgcgtgg cgggattcag tacgatacac gtctggataa
    27361 cgtagcgacc agtaactcca gcattgaata ccgtcgggat gaagaccgtc tggtacagct
    27421 gaattaccgt tacgccagcc cggaatatat tcaggctacg ctgcctaagt actattccac
    27481 tgctgagcaa tataagaatg gtatttcgca ggtaggtgct gtcgccagct ggccaattgc
    27541 cgatcgttgg tccattgttg gggcctacta ctacgacacc aatgcgaaca agcaagccga
    27601 ctctatgtta ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg gttacgagcg
    27661 gaagctgaac ggttgggata acgataaaca acatgcggta tatgacaacg caatcggctt
    27721 taacatcgaa cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag agatgctgcg
    27781 ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt gatttaccac gtaatccgca
    27841 gtgcggttaa ttgaaatgga aaaagtatga agaactggaa aacgctgctt ctcggtatcg
    27901 ccatgatcgc gaataccagt ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg
    27961 tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga
    28021 acgctgctca ggcaaggcag caacttcctg atgacgcgac gctgcgccac caaatcatgg
    28081 aacgtttgat catggatcaa atcatcctgc agatggggca gaaaatggga gtgaaaatct
    28141 ccgatgagca gctggatcag gcgattgcta acatcgcgaa acagaacaac atgacgctgg
    28201 atcagatgcg cagccgtctg gcttacgatg gactgaacta caacacctat cgtaaccaga
    28261 tccgcaaaga gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca
    28321 tcctgccgca ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac gacgccagca
    28381 ctgagctgaa cctgagccac atcctgatcc cgctgccgga aaacccgacc tctgatcagg
    28441 tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt
    28501 tcggtaagct ggcgattgct cattctgccg accagcaggc gctgaacggc ggccagatgg
    28561 gctggggccg tattcaggag ttgcccggga tcttcgccca ggcattaagc accgcgaaga
    28621 aaggcgacat tgttggcccg attcgttccg gcgttggctt ccatattctg aaagttaacg
    28681 acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc catattctgc
    28741 tgaaaccgtc gccgatcatg actgacgaac aggcccgtgt gaaactggaa cagattgctg
    28801 ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc gaaagagttc tctcaggatc
    28861 caggctctgc taaccagggc ggtgatctcg gctgggctac accagatatt ttcgatccgg
    28921 ccttccgtga cgccctgact cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt
    28981 cattcggctg gcatttaatc gaactgctgg atacccgtaa tgtcgataaa accgacgctg
    29041 cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa
    29101 gctggatgca ggaacaacgt gccagcgcct acgttaaaat cctgagcaac taatggttaa
    29161 aacccaacgt gttgtgatca ctcccggcga gcccgccggg attggcccgg acttagttgt
    29221 ccagcttgca cagcgtgagt ggccggtcga actggttgtt tgtgccgatg ccactctcct
    29281 taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt cccccaactc
    29341 ccctgcacaa ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc tacgtgaatc
    29401 tgtcactgtg gggcagttag cgattgaaaa tgggcattac gtggtggaga cgctggcgcg
    29461 agcgtgtgat ggctgtctga acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa
    29521 aggcgtcatt aacgacgcag gcattccgtt taccggtcat accgagtttt tcgaagagcg
    29581 ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc
    29641 aacgacgcat ttaccgctgc gcgatatcgc agatgctatc acccctgcac ttttgcacga
    29701 agtgattgct attttgcatc acgatttgcg tacaaaattt ggtattgccg aaccgcgcat
    29761 tctggtctgc gggctgaatc cgcacgcggg cgaaggcggt catatgggta cggaagagat
    29821 agacaccatt attccggtgc tcgacgagct gcgggcgcag gggatgaaac tcaacgggcc
    29881 gctgcctgcc gataccctgt ttcagccgaa atatctcgat aacgccgacg ccgtgctggc
    29941 gatgtaccac gatcagggtc ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa
    30001 cattacgctg ggcctgccct ttattcgcac atcagtggac cacggcaccg cgcttgaact
    30061 ggcgggacgt ggcgaagccg atgtcggcag ttttattacg gcgcttaatc tcgccatcaa
    30121 aatgattgtt aacacccaat gaataatcga gtccaccagg gccacttagc ccgtaaacgc
    30181 ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca gcattgtttc tgccattaac
    30241 ccgcaaaagg gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt gaccgagccg
    30301 gtcggcgaac gtctggacca gctgacggtc atcgaacttg accgcgatct ggcggcacgt
    30361 ctgcaaacgc atccattctt aggcccgaaa ctgacgattt atcagcagga tgcgatgacc
    30421 tttaactttg gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg
    30481 ccttataaca tctccacgcc gttgatgttc catctgttta gctatactga tgccattgcc
    30541 gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc
    30601 aaagcgtatg gtcgattaag cgtcatggcg caatactatt gcaacgtgat cccggtactg
    30661 gaagttccgc cgtcagcctt tacaccacca cccaaagtgg attccgccgt cgtgcgcctg
    30721 gtccctcatg caacgatgcc tcacccggtt aaagatgttc gcgtgttgag ccgcatcacc
    30781 accgaagcct ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc
    30841 gtcgaggtgt taacgggaat ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc
    30901 gcgcaatatt gccagatggc gaactatctg gcggagaacg cgcctttgca ggagagttaa
    30961 cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag cgtctacatt gaggctcaat
    31021 cttcacctga taatgaacgt tacgtttttg cttataccgt aaccatacgc aatctggggc
    31081 gagcgccagt gcagttgttg gggcgttact ggctgatcac caatggcaat ggccgtgaaa
    31141 ccgaagtcca gggcgaagga gtggttggcg tccagccact tatcgcgcct ggcgaagagt
    31201 accagtacac cagcggtgca atcattgaaa ccccgctggg caccatgcag ggtcactacg
    31261 aaatgatcga tgaaaatggc gtccctttca gcatcgacat tcccgtattc cgactcgccg
    31321 ttcccacact cattcattaa aagaatatgg cgacatacct tattggcgac gttcatggtt
    31381 gttacgatga actgatcgca ttgctgcata aagtagaatt tacccctggg aaagataccc
    31441 tctggctgac gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt ctgcgctatg
    31501 tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg catctgctgg
    31561 cggtatttgc cgggatcagc cgcaataaac cgaaagatcg cctgacaccg ctgctggaag
    31621 cgccggatgc cgacgagctg cttaactggc tgcgtcgcca gcctttgctg caaatcgacg
    31681 aagagaaaaa gttggtgatg gcccacgcag ggatcacgcc gcagtgggat ctacagaccg
    31741 ccaaagagtg cgcgcgcgat gtagaagcgg tgctatcgag tgactcctat cccttctttc
    31801 ttgatgccat gtacggcgat atgccaaata actggtcacc ggaattgcgg gggctgggaa
    31861 gactgcgttt tatcaccaac gcctttaccc gtatgcgttt ttgcttcccg aacggtcaac
    31921 tggatatgta cagcaaagaa tcgccggaag aagcccctgc tccactgaaa ccgtggtttg
    31981 ccattcctgg ccctgtcgct gaagaataca gcatcgcctt tggtcactgg gcatcgctgg
    32041 aaggcaaagg tacgccggaa ggtatttacg cgctggatac aggctgttgc tggggcggtt
    32101 cattaacctg cctgcgctgg gaagataaac agtattttgt ccagccgtcg aaccgacata
    32161 aggatttggg tgaagcggcg gcgtcttaaa cacagcctga tataggaagg ccggataaga
    32221 cgcgaccggc gtcgcatccg gcactagccg taaattctat acaaaattac cgccgctcca
    32281 gaatctcaaa gcaatagctg tgagagttct gcgcatcagc atcgtggaat tcgctgaata
    32341 ccgattccca gtcatccggc tcgtaatccg ggaaatgggt gtcgccttcc acttctgcgt
    32401 cgatatgcgt cagatacagt ttttgcgctt ttggcaagaa ctgttcataa acgcgaccgc
    32461 cgccaatcac catgatttct ggtacgtcac cacacgccgc gatggcttca tccaccgact
    32521 tcacccacgt tacgcgatcg tccgtacccg gttgactgct gaggataata tttttgcgtc
    32581 ctggcaacgg acgaccgatt gattcccagg tatggcggcc cataatcacg ggtttattta
    32641 aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt ccacggcatg gcgttttcca
    32701 tgccgataac gcgatctacc gctaacgccg caatcagact gatcattgag atttcccgat
    32761 aaaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt gcgaggatga
    32821 tgttgaggaa aattttatat tctgctggcg agtccacgct ctctccctgg actcgccgca
    32881 ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca ctctttatta
    32941 ggatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac cttcttcggt
    33001 tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg taatgatctc
    33061 acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt cgttctcaac
    33121 cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg cacgttctcg
    33181 cgcttcatac ggccccagac ctaaactttc cagtgccaga cgcccggttt tcagcgcacc
    33241 ttcgaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa tgtagtggtc
    33301 gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaag tgttctttca ccatctctgt
    33361 cagttgcagg ttggtttgcg gatcgtcgat ggcgttaatc agcacttccg ctttcgccgc
    33421 tcccgcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt tcataccaaa
    33481 tttacgcaag gtttcgatat ggtccggatc gtgatcgagc accaccattt tcaccccgct
    33541 ggagagcagt aaacgtccgg taatctgccc aaaacgaccg aatccggcga taatcacgcg
    33601 cggctgttct tcgtcgatct catcggcttc acgcgcttcc tcagtagaag attgctcaag
    33661 gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca ccgccagggt
    33721 cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcgc caaataccac
    33781 aaaggcaaac tcactgccct gccctaacaa caccgcaaac caacgacgct gtttatttgg
    33841 cacttgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga ggaaaccgag
    33901 cagcaaaatg acaatgcgca atgggttttc aagcagcgtg ccaaagtcta tcgacatgcc
    33961 aacaccgatg aaaaacagcc ccaacagcaa acctttaaat ggttcgatat cgctctccag
    34021 cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc ccatcgccat
    34081 cgacaagccg acctcttcca gcagcaaacc aaagccaaac acgaggaata acgccacggc
    34141 actaaacact tcccgcaagc cagagcgggc tacaaaacgc agcgccggac gcgtgacata
    34201 gcgccccagc aataccacca gcaccagcgc acccgccact tttaacgccg agagagcaaa
    34261 tgcgcccatc gtcgtcgagg cactgctcgt tgccagtagc ggaatcatcg ccaccagcgg
    34321 gatcgccgcg atatcctgga acagcagcac cgcaaaggca ctgcgaccca tttgcgtcac
    34381 catcagattg cgttcattca tcgcctgcat ggcaatcgcc gtagaggaga gcgccagcgt
    34441 catgccgatc aattccgcga cctgccagcg cagcccaagt aacatgcaga acagccccag
    34501 caggccgccg caaatcacca tctgcaatgc gccaccgccg aacactgccg cacgcagctt
    34561 ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca ccaccccaat
    34621 ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc acgggccaat
    34681 aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta ccgcaatggg
    34741 tacaatcagc gctgccgaac cgagataaat cagcgcctga atcagcgtat ggctatccat
    34801 gatgggcctc ctgccattcc agcagacgtt gcttatagtg acgcgcctgc ccttcgaggg
    34861 tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag ttcagcccgc
    34921 agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga tgcgcaccaa
    34981 tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt ttgccatgca
    35041 gcgccgtgcc gccatgaccg taagcccagc cgtgggagaa aactttatcg atccaaagtt
    35101 tgaggagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc agatcggcgc
    35161 gagacagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag agttgataaa
    35221 gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt ttattcgcat
    35281 gggaatgatg cggatacgga tgcgcataaa ttataagaat cataggtagc ccgcgtgatt
    35341 acgcctttgt tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat
    35401 tgattcagaa aagagagaaa tctgaacaat ttaccccatt ctctgcccgc ggatcgtcat
    35461 attgctggtt tgcgccagtg acatccctcg agtctccggg gcaaacgcta cggaaatcaa
    35521 caagccaaac agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt
    35581 gataaagatc ggcagtgccc aggtcgaaac aatggtgcca atacggctta aggacataat
    35641 cacgcccacg gcagaggcgc ggatatctgt cgggaagagt tcattaggat agagccactg
    35701 caaattaccc ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca
    35761 gatccccata tccgggatca gccccaaaac cgccagcgcc agcgtcatca tggcaaagct
    35821 gccaatcaac aatggacgcc gtccggcagt gtttaaccac agcatcggcg gaatacagcc
    35881 gagcataaag aacaggctaa tcaccacgtt ccccagcgcc gcgtttttgc caacacccaa
    35941 tcccaacaaa ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg
    36001 gcaggtccag atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc
    36061 gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt
    36121 tatcatcatc tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc
    36181 aggcagttcg aatcgaccaa tcaaaatcaa caaacagggg atcgccgcgc tacccagcat
    36241 ccagcgccag ccgccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc
    36301 gccgacatac cacatggcgg cgataaagct gatggaaaat gcccgctgac gggtactgga
    36361 gaactcggtg atcattgagg tggcgatggg ataatctgca ccgatgacaa tgccgataag
    36421 tacccgcatc accaacagtt cgacggggga tgaaacaaac atcgtcgcca ccgatatcac
    36481 gccgatggcg atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggaaatata
    36541 gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca acaagccaat
    36601 ccagtcagcg tccagtttta gcgccggagt cagttgctcc agcgccacgc caatcattac
    36661 cagtacataa ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat
    36721 tgaggagaat ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt
    36781 tgcaatgaga gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca
    36841 caccaaaggt gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc
    36901 gacaggtgcc gcactccaga catccggcat aatcgaagcg cacactgccg tcatcctgct
    36961 tcttgtacag acctgcgggg cacgctttca ccagcagctc cagggcctgt ttatcggcat
    37021 cggccttcac aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt
    37081 tgacgtccac attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat
    37141 cagattgatg aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg
    37201 cgcactgccg tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc
    37261 gctaaacatg cgtgggttat caaggaaggc cggtagtttc tggtacatac gcatatcgcg
    37321 cagcgggcca ctctcaagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc
    37381 gcttttcatc gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc
    37441 cataccgcga atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa
    37501 tacgccgtca ccaaccaact ccggcagcat gttgatgcct gcttccggca ctacgtgagc
    37561 ggaatattcc accagcttgc cacccgcgat cagcggtgca acggccggat gttgtttgaa
    37621 atcttccagc atttgcggca ccgatttttt tgcgtcatgc agatgatgca gaccacaaac
    37681 cagccccagc gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc
    37741 ggtgggtgat cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc
    37801 ttcaataacc gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg
    37861 tttaacgcgt tttgccatcc ccagcttttc ggcaaggatg gagttcaccc catcagcaag
    37921 gatcatcgtt ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc
    37981 gcgctgtacg aggttatcga cgcggatccc ggttattaac tgcgcgcccg cttcttcggc
    38041 ctgctccatc aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatgg
    38101 cgaggtttcg tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc
    38161 gagtttttca tgggtgatca ggcgttctac cggggcggat tcggcgaaac caggaataat
    38221 gtgttccagg ctgtgggcat agagacgccc gccggtgacg ttcttggcac ctgcggaatt
    38281 gccacgctcg ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga
    38341 accggcaagc ccagcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc
    38401 ctgtcagcgg cagggccaaa atgcagccct gccagagtgg atcaacgcgc taaagctgcg
    38461 gtcagcgccg gaaggatctt cacggcgtcg ccaacaatgc cgtaatccgc gtactggaag
    38521 atcggcgcat ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc
    38581 atgtgctgga tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg
    38641 ttggagatac cgacatagcg ttcgtgctcc atccattttt cgttttccgc caccggacga
    38701 gaacaggcca actccgcacc tatcgccttg caaagctgtt ctgccagcgc aatgttctct
    38761 ttgctgccaa taccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg
    38821 ttgctctggc gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc
    38881 acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg
    38941 ctgatggtca gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccaccg
    39001 taaaccatgt gtttcactgt cgctttaccg tcctgtacgc tgacggtgct ggcatcgtta
    39061 gacaccgccg ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg
    39121 tttggcagca gcaccaggcc gtctgcgccg tgctggcgaa tagtgtcagc catgacaccg
    39181 gcgtaatctt cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg
    39241 ccgagctgga ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta
    39301 gctaaagcct gcgcaccgtt catcagttcc ggcagacgag aaggggtatc gctgaatacc
    39361 cagacttgag aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt
    39421 cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc
    39481 gctgtttcgg cgcagcaacc tgttgttcag accaggcgtc cacggcgtta aaaccgatat
    39541 ccgccgccga ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag
    39601 gaatttgtgg ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta
    39661 aggtttcggt ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga
    39721 taattttgct gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa
    39781 cctgctgggc ataaaggtcg gaagaaccat cgccacagag gatcaaatca aagcctgctt
    39841 tctgggcggc tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt
    39901 catcaatcac cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg
    39961 cgttggtcag ggctttaccg cccacactta aggccgtcac ctgcgcctct gctgcctgtt
    40021 gctttagctg gcaagccgct tcaatagcgt tcagatcgta ttggcttatt ttggcatcgg
    40081 ctttgctgaa gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca
    40141 cgcacttata gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt
    40201 tcatatcggt ggtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat
    40261 caccaatatt gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc
    40321 accaatattg aaaacatggc gtagaaaaaa gaaattttca atattgcttt atggatcacc
    40381 aatattgaaa gtcagatgct atttttttac tcacaacaca acataacaaa ctgattaata
    40441 atcaaaatta acgagaaaag cgccctctat tcacccgaaa caaaaatgtg ataacaatca
    40501 cagaatgcag cttattgaat acccattatg ggttagccat taacgcgtcc acgaggttaa
    40561 taataattat atcaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa taaatattcg
    40621 ttttcacagt ggaattaact catgaagaat gaaaagagaa aaacgggaat agaaccgaag
    40681 gttttctttc cgccgttaat aatcgtcggc atactttgtt ggcttacagt cagagatctg
    40741 gatgcagcga atgtcgttat taatgctgta ttcagttacg tcaccaatgt atggggatgg
    40801 gcatttgaat ggtatatggt ggtgatgctt ttcggttggt tctggctggt gtttggcccg
    40861 tatgccaaaa agcgtttagg taacgaacca cctgaattta gtaccgccag ttggatcttt
    40921 atgatgttcg cctcctgtac gtctgctgcc gtactgttct ggggatcgat tgagatctac
    40981 tactacatct ccaccccgcc gtttggctta gaaccgaact cgacaggggc gaaagagttg
    41041 gggctggctt acagcttgtt ccactgggga cctctgccgt gggccactta cagcttcctt
    41101 tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg ccccagctcg
    41161 acactggtgc cgctggtagg tgaaaaacac gccaaagggt tgttcggcac tatcgtcgac
    41221 aacttctatc tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct tgccacgccg
    41281 ctggtgaccg agtgtatgca atggttgttt ggcattccgc ataccctgca actggacgct
    41341 atcatcatta cctgctggat tatcctcaac gccatttgcg tcgcttgcgg tctgcaaaaa
    41401 ggggtacgta tcgccagtga cgtgcgtagt tacctgagct tcctgatgct gggttgggtg
    41461 ttcattgtca gcggtgccag cttcatcatg aactacttca ccgattcggt ggggatgttg
    41521 ctgatgtatc tgccgcgcat gttgttctat accgatccca tcgctaaagg cggcttcccg
    41581 cagggctgga ccgtgttcta ctgggcatgg tgggtgattt atgctatcca gatgagtatc
    41641 ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg catggtgctg
    41701 gggctgacag cgtcaacctg gatcctgtgg actgtactcg gtagtaacac tctgctgttg
    41761 atagataaaa acatcatcaa cattccaaat ctgatcgaac agtacggtgt ggcgcgcgcc
    41821 atcattgaaa cctgggccgc tctgccactc agcaccgcca ccatgtgggg cttcttcatc
    41881 ctctgcttta ttgccaccgt tacgctggtt aacgcctgct cttataccct ggcgatgtcc
    41941 acttgccgcg aagtacgcga tggtgaagaa ccacctctgc tggtgcgtat cggttggtca
    42001 attctggttg gcattatcgg tattgttctg ctggcgctcg gcggcctgaa accgattcaa
    42061 accgccatta tcgccggagg atgcccgctg ttcttcgtca acattatggt gacgctctcc
    42121 tttattaaag acgcgaaaca gaactggaaa gattaattaa cccccaaaat atcaagaggt
    42181 tgaaagatgg attttaattt aaatgatgag caggaactgt ttgtcgccgg tatccgcgaa
    42241 ctgatggcca gcgaaaactg ggaggcctat tttgccgagt gcgaccgtga cagcgtctac
    42301 ccggaacgtt ttgtcaaagc actggcggat atgggtatcg acagtctgct gatccctgaa
    42361 gagcacggtg gtctggacgc ggggtttgtt actctcgccg ccgtgtggat ggagctggga
    42421 cgtctggggg caccaaccta tgtgctgtac cagttgccgg gcgggttcaa caccttcctg
    42481 cgcgaaggca cacaagagca gatcgacaag attatggctt tccgcggcac cggtaagcag
    42541 atgtggaact cagcgattac cgaaccgggc gcgggctccg acgtgggtag cctgaaaacg
    42601 acttataccc gtagaaatgg taagatttat cttaatggta gtaagtgttt tattaccagc
    42661 agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc ggacaaacct
    42721 gtctacaccg aatggtttgt tgatatgagc aaaccgggca tcaaagtgac caaacttgaa
    42781 aagctcggtc tgcgtatgga tagctgctgt gaaatcacct ttgacgacgt ggaactggac
    42841 gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga agagttcgac
    42901 catgaacgtt tcctggtagc cctcaccaac tacggtacgg cgatgtgcgc ctttgaagat
    42961 gcggcgcgct acgccaatca gcgcgtgcag tttggcgagg ctattggtcg tttccagttg
    43021 attcaggaaa aattcgccca catggcgatc aaattaaact ccatgaaaaa catgctgtat
    43081 gaagcagcgt ggaaagcaga caacggcacc atcacctctg gcgatgcagc gatgtgcaaa
    43141 tacttctgcg ccaatgcggc atttgaagtt gtggatagcg caatgcaggt gctgggcggt
    43201 gtcgggattg cgggcaacca ccgcatcagc cgcttctggc gtgatctgcg tgtagaccgc
    43261 gtctccgggg gatctgacga aatgcagatc ctgacgctgg gtcgcgcggt gctgaagcaa
    43321 taccgctaag tccgtgcgct gcctgatgcg acgctaatgc gtcttatcag gcctacggga
    43381 gcacgaatgt aggccggata aagcgtttac gccgcatccg gcagtcatgc gctacataaa
    43441 tttttcagga gctaataatg gatcatctac ccatgccgaa attcgggcca ttggccggat
    43501 tgcgcgttgt cttctccggt atcgaaatcg ccgggccgtt cgccgggcaa atgttcgcag
    43561 aatggggtgc ggaagttatc tggatcgaga acgtcgcctg ggccgacacc attcgcgttc
    43621 aaccgaacta cccgcagctc tcccgccgca atttgcacgc gctgtcgtta aatattttca
    43681 aagatgaagg ccgcgaagcg tttctgaaat taatggaaac caccgatatc ttcatcgaag
    43741 ccagcaaagg tccggccttt gcccgtcgtg gcattaccga tgaagtactg tggcagcaca
    43801 acccgaaact ggttatcgct cacctgtccg gttttggtca gtacggcacc gaggagtaca
    43861 ccaatcttcc ggcctataac accatcgccc aggccttcag tggttacctg attcagaacg
    43921 gtgatgttga ccagccaatg cctgccttcc cgtataccgc cgattacttt tctggcctga
    43981 ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg tgaaaccggt aaaggcgaaa
    44041 gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc atgatggatt
    44101 acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg taaagatccc tactacgccg
    44161 gttgcggtct gtataaatgt gccgacggct acatcgtgat ggaactggtg ggcattaccc
    44221 aaattgaaga gtgctttaaa gatattggcc tcgcacatct gcttggcacg ccagaaatcc
    44281 cggaaggcac tcagcttatc caccgtatcg aatgccctta cggcccactg gttgaagaga
    44341 aactcgatgc ctggctggcg acacatacca tcgcggaagt aaaagaacgc tttgctgaac
    44401 tgaatatcgc ctgcgccaaa gtgctgaccg taccggaact ggaaagcaat ccacagtatg
    44461 tggctcgcga atcaatcact cagtggcaaa cgatggatgg tcgcacctgc aaagggccga
    44521 acatcatgcc gaaattcaaa aataaccccg gacaaatctg gcgcggaatg ccctcacatg
    44581 gcatggacac ggctgccatt ttgaaaaata tcggctacag cgaaaacgac attcaggagt
    44641 tggtcagcaa aggtctggcc aaagttgagg actaaacgcg tggcgtcagt cggcttgcct
    44701 gactagcgcc cgcccaataa caaacgaaca aaaatggata gaggtgcaat ggatatcatt
    44761 ggcggacaac atctacgtca aatgtgggac gatcttgcgg acgtttacgg tcataaaacg
    44821 gcgctgattt gtgaatccag cggcggagtc gttaaccggt atagttatct tgagttaaat
    44881 caggagatta accgcacggc aaacctgttt tatacgctgg ggattcgcaa aggcgacaag
    44941 gttgcactac atctcgacaa ctgcccggaa tttatctttt gctggttcgg gctggcaaaa
    45001 attggcgcga ttatggtgcc gattaacgcc cgcctgttac gcgaggaaag cgcgtggatc
    45061 ctgcaaaata gccaggcgtg cctgctggtg accagtgcgc aattctatcc tatgtatcaa
    45121 cagattcagc aggaagatgc cactcaattg cggcacattt gcctgataga tgtggcactt
    45181 cccgctgatg atggcgtgag ttcgtttact caactgaaaa atcaacaacc tgccaccttg
    45241 tgctatgcac cgccgctatc gactgacgat acggcggaaa ttctcttcac ctccggcacc
    45301 acctcccgac cgaaaggtgt ggtgattacc cattacaacc tgcgcttcgc tggatattac
    45361 tccgcctggc agtgtgcact gcgtgacgat gacgtctacc tgacggtaat gcctgcgttt
    45421 catatcgatt gccagtgtac tgcggcgatg gcggcgtttt ctgccggggc cacctttgtg
    45481 ctggtcgaga aatacagcgc ccgcgccttc tggggacagg tacagaagta ccgcgccacc
    45541 gttaccgaat gtattccgat gatgatccgt accttgatgg tgcagccgcc ttcagcgaac
    45601 gatcggcaac accgcctgcg ggaagtgatg ttttatctca acttgtcgga gcaggaaaaa
    45661 gatgcgtttt gtgaacgctt cggcgttcgc ttgctgacgt cttatgggat gacggaaacc
    45721 attgtgggca ttatcggcga tcgccctggc gataaacgac gctggccgtc gattggtcgg
    45781 gcggggtttt gctacgaagc ggagatccgc gacgatcaca atcacccgct cccggcaggt
    45841 gagatcggtg aaatctgtat taaaggcgta ccagggaaaa ccatcttcaa agagtatttt
    45901 ctcaacccga aagccactgc gaaagtgctg gaagccgacg gctggctaca taccggcgat
    45961 accggatact gcgacgaaga gggctttttt tatttcgtcg atcgccgctg caatatgatc
    46021 aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa atatcatcgc cacccatcca
    46081 aaaattcagg atatcgtggt tgtgggtatt aaagattcga ttcgcgatga agccatcaaa
    46141 gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag aggaattttt ccgcttctgc
    46201 gaacaaaata tggcgaaatt taaagtgccc tcttatctgg agatcagaaa agatctgcca
    46261 cgtaattgct cggggaaaat aattagaaag aatctgaaat aaatgaaaca gcagggaact
    46321 accctgccag ctaataatca cgctataaaa caatatgcat tttttgcagg gatgttgtcg
    46381 tccctgaaaa agcaaaaatg gagaaaagga atgagtgaat cattacatct gacccgcaat
    46441 ggatcaattc tggaaattac ccttgatcgt ccaaaagcga atgctattga tgcaaaaacc
    46501 agctttgaaa tgggcgaagt atttctaaat ttccgtgacg atccgcaatt acgtgtcgcc
    46561 attattaccg gtgccggaga gaaattcttt tccgcgggct gggatttaaa agcggcagca
    46621 gaaggcgaag caccggatgc tgactttggc ccgggtggat ttgcgggatt aaccgaaatt
    46681 ttcaatctcg acaaaccggt tatcgcagct gtgaacggct atgcctttgg cggcggtttc
    46741 gaactggcgc tggcggcaga ttttattgtt tgtgccgata acgccagctt cgccctgccg
    46801 gaagccaagc tgggcatcgt tcctgacagc ggcggcgtgc tgcgtctacc gaagatcctg
    46861 ccgcctgcca tcgtcaatga aatggtgatg accggcagac gaatgggcgc agaagaggcg
    46921 ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg aactgatgga taacgcccgc
    46981 gaactggcgc agcagctggt taacagcgcc ccgctggcga ttgcggcgct gaaagagatc
    47041 ttccgcacca ccagcgaaat gccggtagaa gaagcgtatc gctatattcg cagcggcgtg
    47101 ttgaaacact atccgtcggt tctgcattcg gaggatgcca ttgaagggcc gctggcgttg
    47161 ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt acgcctttga
    47221 ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg tcttgattgg
    47281 cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc gtggtgacta
    47341 cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta tgcatggcta
    47401 ctgcgacact gacaccatcg ttggggaaaa cggtcatatc gggcacggag cgatcctgca
    47461 tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtgatta tggatggcgc
    47521 ggtcattggc gaagagagca ttgttgccgc catgagcttt gtcaaagcag gctttcacgg
    47581 cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg ttagtgacga
    47641 cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg ggcgctgtca
    47701 tgcatcgtta catgaaacac agccactgag gcaaatggag gaaaatcgcc cccgtttgca
    47761 ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcgacctg gttgctggat
    47821 aagatgctac aacatcgcat ctggcaagca ggacgcccgg tatcaacgag ataaaattaa
    47881 cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag ttccgtgagc
    47941 ggaacggcag gaatacgcgg cgtctttctt acgccgacta atttatcccg gctgttattg
    48001 cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt ttaccagtcg ctggcactgg
    48061 catccccgtc cttccagctt attagggatc atcttaactt cgcagcttat ttctgcgact
    48121 tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc gaaatgaata
    48181 gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc gattaacaag
    48241 tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca aattaaaagt
    48301 ctggctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt ggatatatcg
    48361 atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt catcaaaaat
    48421 atcgattccc caaaagattt acttaatggg caaaaaaaac ggtcctcatc agagaaccgt
    48481 attacttatt cgatcaaatt agcggatgaa aaatatccgc catgacacgc tattatttga
    48541 tctgcgcgtg catttcctgc accgaaatta ctttttcggt cgcgtcggca ttcagcgcca
    48601 tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac
    48661 tgcgacggat cacgcgggag tcttcaatcg cgcgacggcc tgaagtggtg ttgatgatgt
    48721 aggtatattc gccattcttg atgcggtcct gaatgtgcgg acggccttca tgcaccttgt
    48781 tcaccagacg cggattaatg cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat
    48841 ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc
    48901 cttcgcgcac ggaaagcaac gcacgaccgt gtttcttcat ggtggagttg ctgcctagct
    48961 gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc catcacttcc ccggtggagc
    49021 gcatttctgg ccctaacagc gggtcaacac ccgggaattt attgaacggc agcaccactt
    49081 ctttcaccga gtagtacggc gggataactt ctttggttac gccctgctca gccagcgatt
    49141 tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga
    49201 acggaacggt acgcgccgca cgcgggttaa cttcaatcag gtagacttcg ttgtttttca
    49261 ccgcaaactg cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct
    49321 gctggcgcat cacatcctga atttcctgac ttaaggtgta ggctggcaga gaacatgcgg
    49381 agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca
    49441 tttcgccgtc gcagatggca tccacgtcaa cttctaccgc gtcatcaagg aagtgatcca
    49501 gcaacactgg cgcatcgtta gacacgctga ccgccgtctg gaagtagcga cgcaggtcag
    49561 cttcgtcata gacgatttcc atcgcccgac cgccgagaac ataagacgga cgtaccacca
    49621 gcgggtagcc aatctctttc gccttctcta ccgccatttc aatagcggta acggtggcgt
    49681 tcgccggttg tttcagtttc agacgctcaa ccgcatgctg gaagcgttca cggtcttctg
    49741 cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcagct tccagcgcgc
    49801 gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa
    49861 tacgcacgat ttctagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag
    49921 tgtcgtagtc ggtggagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt
    49981 cttcgcgcag cgccagcgag gcgtgtacac agcagtagtc gaattcgata ccctgaccga
    50041 tacggttcgg gccgccgccg agaaccatga ttttttcacg gtcggtagac ggattcgctt
    50101 cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac tctgccgcac
    50161 aggtatccac gcgcttataa accgggtgca ggtcatactg gtcgcgcagc ttgcggattt
    50221 ccgcttcgcg cacgcccgcc agttttgcca gacgcgcatc ggcaaagcct ttgcgtttca
    50281 gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgctact ttctcttcca
    50341 gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatattggtc aggttgaaca
    50401 cgccgtccac agacaggccc gcacggaagg catcggcgat gtaccagata cgctctgcgc
    50461 cagcatcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatcg aggctcactt
    50521 tcgggtcgaa tccagtcgca ccgacttcca ggccgcgcag cgctttttgc agggattcct
    50581 gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt
    50641 cgttagcacc agcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga
    50701 tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga
    50761 gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg
    50821 acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt
    50881 ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca
    50941 gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg
    51001 gcgcgacagt gatggagtca ccggtatgga tgcccatcgc atcaaagttt tcgatagagc
    51061 agacgatgat gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc
    51121 cgatcagcga ctcatcaatc agcagctctt tggttgggga gagatccaga ccgcgggcgc
    51181 aaatttcttc aaactcttcg cggttataag cgataccgcc gccgctaccg cccatggtaa
    51241 aggatgggcg aataatgcac gggaagccca cgtcagcggc aaccgccagc gcttcttcca
    51301 tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga
    51361 aacgacggcg gtcttctgct ttatcaatcg catcggcggt cgcaccaatc atagtcacgc
    51421 cgaactcttc caacacgccc tgacgctcca gctccagcgc gcagttcagc gccgtctgac
    51481 cgcccatcgt tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtactactt
    51541 cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg
    51601 ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt
    51661 gcgcgccaga gtagtcaaac tcacatgcct gaccgataac aatcgggccc gcacccagaa
    51721 tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc
    51781 ggttttacgg tactgctcaa ttaactcgat aaagtggtcg aacaacggcg ctgcgtcgtg
    51841 tggcccaggg ctggcttccg ggtgaccctg gaagctgaac gccggtttat cagtgcgatg
    51901 aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa
    51961 tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc
    52021 cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc
    52081 accgctcgcc agcgccagca gctgatgacc gaggcagatg ccgaataccg gaatatcggt
    52141 ttcgaggaat ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc
    52201 gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc
    52261 cggaacgatg gtcaggcgac aacctctatc caccagcatc cgcaggatgt tgcgcttggc
    52321 accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag
    52381 gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc
    52441 ttttgccaga tccatgccat tcagacctgg gaacgcgcgg gctttttcta acgccaacgc
    52501 cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa
    52561 acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta
    52621 agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcagat cgcgaatcac
    52681 cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc
    52741 aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc
    52801 ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc
    52861 tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa
    52921 aacaccctcc agagaatatt cactcacttt atttgcatat taattcacaa tgatgacgta
    52981 aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg
    53041 ggcgctaaaa gtcaacttaa tggcgggatt ttttatatta ttttgtggct catgcatgtt
    53101 ttacaggcat taaacgacaa aaagatcata taacctgttc tggaaaacgc ttgcgcagca
    53161 agaagattgc atgaaataaa aacgagcagg aaaaagtgga ccaaatggtc aaaatttaca
    53221 ttaaaacaac aaaaacagaa gacaaaacat gtaatttttg gcacttatag aatatttaaa
    53281 aacataaca
//
LOCUS       DAJSKJ010000008        50377 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000008 DAJSKJ010000000
VERSION     DAJSKJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.008"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(101..1291)
                     /locus_tag="ON345_000395"
     CDS             complement(101..1291)
                     /locus_tag="ON345_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3070507.1"
                     /translation="MLTDTRLRHLKPKEKLYKVNDRDGLYVAVTPAGTISFRYNYSIN
                     GRQETVTFGRYGVGGITLAEARERLNEAKKMVASGRSPAREKARDKARIKDAETFGAW
                     AEKWLRGYQMAESTRDMRRSVYQRELKSKFAQQKLTEITHEDLRALTDNIVERGAPAT
                     AVHAREIVLQVYRWAIERGQKVENPADLVRPASIAKFEPRDRALTPVEIGLMYRYMER
                     VGTTPSIRAAVKLLLLTMVRKSELTNATWNEINFSEALWTIPKERMKRRNPHLVFLSR
                     QAMDIMIALKTFAGSSDFILPSRYDSDAPMSSATLNRVLTLTYRLAQKEGELLPKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRAEMLQDWANMIDE
                     WTSK"
     gene            complement(1598..3175)
                     /gene="guaA"
                     /locus_tag="ON345_000396"
     CDS             complement(1598..3175)
                     /gene="guaA"
                     /locus_tag="ON345_000396"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3070508.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGICYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(3244..4710)
                     /gene="guaB"
                     /locus_tag="ON345_000397"
     CDS             complement(3244..4710)
                     /gene="guaB"
                     /locus_tag="ON345_000397"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR3070509.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4872..6242
                     /gene="xseA"
                     /locus_tag="ON345_000398"
     CDS             4872..6242
                     /gene="xseA"
                     /locus_tag="ON345_000398"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR3070510.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNEFDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(6239..6454)
                     /locus_tag="ON345_000399"
     CDS             complement(6239..6454)
                     /locus_tag="ON345_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR3070511.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6523..7995)
                     /gene="der"
                     /locus_tag="ON345_000400"
     CDS             complement(6523..7995)
                     /gene="der"
                     /locus_tag="ON345_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR3070512.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(8113..9291)
                     /gene="bamB"
                     /locus_tag="ON345_000401"
     CDS             complement(8113..9291)
                     /gene="bamB"
                     /locus_tag="ON345_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR3070513.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(9302..9922)
                     /gene="yfgM"
                     /locus_tag="ON345_000402"
     CDS             complement(9302..9922)
                     /gene="yfgM"
                     /locus_tag="ON345_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR3070514.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSTSLAYQNAITAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDSIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9940..11214)
                     /gene="hisS"
                     /locus_tag="ON345_000403"
     CDS             complement(9940..11214)
                     /gene="hisS"
                     /locus_tag="ON345_000403"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR3070515.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11325..12443)
                     /gene="ispG"
                     /locus_tag="ON345_000404"
                     /gene_synonym="gcpE"
     CDS             complement(11325..12443)
                     /gene="ispG"
                     /locus_tag="ON345_000404"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR3070516.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAVGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12470..13483)
                     /gene="rodZ"
                     /locus_tag="ON345_000405"
     CDS             complement(12470..13483)
                     /gene="rodZ"
                     /locus_tag="ON345_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR3070517.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPASTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEHS
                     PAQ"
     gene            complement(13768..14922)
                     /gene="trmG/rlmN"
                     /locus_tag="ON345_000406"
     CDS             complement(13768..14922)
                     /gene="trmG/rlmN"
                     /locus_tag="ON345_000406"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR3070518.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(15072..15503)
                     /gene="ndk"
                     /locus_tag="ON345_000407"
     CDS             complement(15072..15503)
                     /gene="ndk"
                     /locus_tag="ON345_000407"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR3070519.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15652..17964)
                     /gene="pbpC"
                     /locus_tag="ON345_000408"
     CDS             complement(15652..17964)
                     /gene="pbpC"
                     /locus_tag="ON345_000408"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR3070520.1"
                     /translation="MLRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKITTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NSIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17965..22926)
                     /gene="yfhM"
                     /locus_tag="ON345_000409"
     CDS             complement(17965..22926)
                     /gene="yfhM"
                     /locus_tag="ON345_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR3070521.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLNADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            23133..23978
                     /gene="sseA"
                     /locus_tag="ON345_000410"
     CDS             23133..23978
                     /gene="sseA"
                     /locus_tag="ON345_000410"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR3070522.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRCVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24471..25247)
                     /gene="sseB"
                     /locus_tag="ON345_000411"
     CDS             complement(24471..25247)
                     /gene="sseB"
                     /locus_tag="ON345_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR3070523.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25390..26673)
                     /gene="pepB"
                     /locus_tag="ON345_000412"
     CDS             complement(25390..26673)
                     /gene="pepB"
                     /locus_tag="ON345_000412"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR3070524.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26851..27051)
                     /gene="iscX"
                     /locus_tag="ON345_000413"
     CDS             complement(26851..27051)
                     /gene="iscX"
                     /locus_tag="ON345_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR3070525.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27063..27398)
                     /gene="fdx"
                     /locus_tag="ON345_000414"
     CDS             complement(27063..27398)
                     /gene="fdx"
                     /locus_tag="ON345_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR3070526.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27400..29250)
                     /gene="hscA"
                     /locus_tag="ON345_000415"
     CDS             complement(27400..29250)
                     /gene="hscA"
                     /locus_tag="ON345_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR3070527.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29267..29782)
                     /gene="hscB"
                     /locus_tag="ON345_000416"
     CDS             complement(29267..29782)
                     /gene="hscB"
                     /locus_tag="ON345_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR3070528.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLTSEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29878..30201)
                     /gene="iscA"
                     /locus_tag="ON345_000417"
     CDS             complement(29878..30201)
                     /gene="iscA"
                     /locus_tag="ON345_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR3070529.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30218..30604)
                     /gene="iscU"
                     /locus_tag="ON345_000418"
     CDS             complement(30218..30604)
                     /gene="iscU"
                     /locus_tag="ON345_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR3070530.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30632..31846)
                     /gene="iscS"
                     /locus_tag="ON345_000419"
     CDS             complement(30632..31846)
                     /gene="iscS"
                     /locus_tag="ON345_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3070531.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRADTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31958..32446)
                     /gene="iscR"
                     /locus_tag="ON345_000420"
     CDS             complement(31958..32446)
                     /gene="iscR"
                     /locus_tag="ON345_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR3070532.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32747..33484)
                     /gene="trmJ"
                     /locus_tag="ON345_000421"
     CDS             complement(32747..33484)
                     /gene="trmJ"
                     /locus_tag="ON345_000421"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR3070533.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33603..34406
                     /gene="suhB"
                     /locus_tag="ON345_000422"
     CDS             33603..34406
                     /gene="suhB"
                     /locus_tag="ON345_000422"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR3070534.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34551..35405
                     /locus_tag="ON345_000423"
     CDS             34551..35405
                     /locus_tag="ON345_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3070535.1"
                     /translation="MALPVNKRVLKIMFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35596..36876
                     /gene="csiE"
                     /locus_tag="ON345_000424"
     CDS             35596..36876
                     /gene="csiE"
                     /locus_tag="ON345_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR3070536.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTAITATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKHLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36868..38007)
                     /locus_tag="ON345_000425"
     CDS             complement(36868..38007)
                     /locus_tag="ON345_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR3070537.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38167..39057)
                     /gene="hcaR"
                     /locus_tag="ON345_000426"
     CDS             complement(38167..39057)
                     /gene="hcaR"
                     /locus_tag="ON345_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR3070538.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39193..40554
                     /locus_tag="ON345_000427"
     CDS             39193..40554
                     /locus_tag="ON345_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR3070539.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDITWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40551..41069
                     /locus_tag="ON345_000428"
     CDS             40551..41069
                     /locus_tag="ON345_000428"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR3070540.1"
                     /translation="MSAQVSQELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            41069..41389
                     /locus_tag="ON345_000429"
     CDS             41069..41389
                     /locus_tag="ON345_000429"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR3070541.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41386..42198
                     /gene="hcaB"
                     /locus_tag="ON345_000430"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41386..42198
                     /gene="hcaB"
                     /locus_tag="ON345_000430"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR3070542.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42208..43410
                     /locus_tag="ON345_000431"
     CDS             42208..43410
                     /locus_tag="ON345_000431"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR3070543.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSNNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            43507..43929
                     /locus_tag="ON345_000432"
     CDS             43507..43929
                     /locus_tag="ON345_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR3070544.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43977..44849)
                     /locus_tag="ON345_000433"
     CDS             complement(43977..44849)
                     /locus_tag="ON345_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR3070545.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPDKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44861..45955)
                     /locus_tag="ON345_000434"
     CDS             complement(44861..45955)
                     /locus_tag="ON345_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR3070546.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRGVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45988..46986)
                     /locus_tag="ON345_000435"
     CDS             complement(45988..46986)
                     /locus_tag="ON345_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3070547.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINDIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(47011..48522)
                     /locus_tag="ON345_000436"
     CDS             complement(47011..48522)
                     /locus_tag="ON345_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3070548.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIALVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48545..49528)
                     /locus_tag="ON345_000437"
     CDS             complement(48545..49528)
                     /locus_tag="ON345_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3070549.1"
                     /translation="MPTKIRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASGAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49625..>50377)
                     /locus_tag="ON345_000438"
     CDS             complement(49625..>50377)
                     /locus_tag="ON345_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR3070550.1"
                     /translation="YLKRDDMTAELLNLWHLTGQADKAADILATRKFHPWEGGEGKVT
                     SQFILNQLLRAWQHLDAREPQQASELLHAALHYPENLSEGRLPGQTDNDIWFWQAICA
                     NAQGDETEAMRCLRLAATGDRTINIHSYYNDQPVDYLFWQGMALRLLGEQQTAQQLFS
                     EMKQWAQEMAKTSIEADFFAVSQPDLLSLYGDLQQQHKEKCLMVAMLASAGLGEVAQY
                     ESARAELTAINPAWPKAALFTTVMPFIFNYVH"
BASE COUNT        11869 a        14045 c        12486 g        11977 t
ORIGIN      
        1 tccagataaa accggtaaag attcagtaaa ttttagcggc taaaattgct ctcctggcgt
       61 agatacaaga agtatatcca cttaaattac tgattattaa ctacttacta gtccattcat
      121 caatcatatt cgcccaatct tgtagcatct cggccctctg ctcacgatat tcagccttgt
      181 tataaacagc tcgtaccccc ttttgttcat gggcaaggca cttctcaatc cagtcagtat
      241 tgtacccggc ctcatgtagc aaggtgctgg cagtacgccg taagtcatgg ggaccaaact
      301 taggtaacaa ctccccttct ttctgagcca gacgatacgt cagtgtcaaa acccggttta
      361 aagtagcgct actcattggc gcatcggaat cgtaccgcga aggaaggata aaatcagagc
      421 taccggcaaa ggttttgaga gcaatcataa tatccattgc ctgtctggaa agaaaaacca
      481 aatgtggatt acgtcgtttc atcctctcct ttggtatcgt ccataatgcc tcactaaaat
      541 tgatctcgtt ccaggttgcg ttagtaagct cacttttacg taccatcgtt aacagcaaaa
      601 gtttaaccgc tgctctgatt gatggcgtcg ttcctacccg ttccatgtac cgatacatca
      661 gaccaatttc aactggcgtc aatgccctgt cacgaggctc aaatttcgct atgcttgcag
      721 gccgtaccag atcagcggga ttctcaacct tctgaccgcg ctcaatagcc cagcgataga
      781 cttgcaatac aatctctctg gcgtgcacag ctgtcgccgg tgcccctctc tcgacaatgt
      841 tatcggttaa tgcgcgtaag tcttcatgtg taatctcggt caatttctgc tgcgcaaatt
      901 ttgacttcaa ctccctttga tataccgaac gccgcatatc acgcgtcgat tcagccattt
      961 gataaccgcg taaccatttc tcagcccatg caccaaaagt ctccgcatct ttgatacgcg
     1021 ctttatctct agctttttcc ctcgctggcg atcttccact ggcaaccatt tttttagctt
     1081 cattgagccg ttcacgcgct tctgcaagcg tgatccctcc aacaccatag cggccaaaag
     1141 taacggtctc ctgtcttcca tttattgaat agttataacg aaatgaaata gttccagccg
     1201 gagtgaccgc aacatacaga ccatcacggt cattaacttt atagagtttc tccttcggct
     1261 taaggtgacg cagcctggtg tcagtcaaca tgttttattg ttttccttta tcaaaaatac
     1321 catgttgtaa aaaattcaga aaagttgttt aactctctgt ttttaattga gttataaaaa
     1381 ataaatacca taactcaacc gaaatttatc acatggtact ttttcaaacc tgaattcgaa
     1441 accagagagt accatcaaaa attccattga aaaaaccgtg cttcccgttg ctaacagctg
     1501 ccagaaagtg ccagatacaa aaacaaaaaa gcccgcagtt acgcgggctt agaggtactt
     1561 tactgctttt acgtgcaggc tgttgccaga cgttaaatca ttcccactca atggtagctg
     1621 gcggcttgcc gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc
     1681 ggttggaaac gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt
     1741 cgatggtttc gacagcacgc agagagacaa cccagtcata cttacgacca tcgcccatta
     1801 cgccaacgga acgtaccggc aggaacacag tgaacgcctg gctgactttg tcgtacaggt
     1861 ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact
     1921 ctttcttcac ttcacccagc acacgaacgc caaggcctgg tcccgggaac gggtgacggt
     1981 acagcatgtc gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca
     2041 gctctttcag cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt
     2101 ggtgagattt gatgacgtgt gctttaccgg ttgcagaagc cgcagattcg ataacgtcag
     2161 ggtagatggt gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga
     2221 acacttcaac gaaaacgcgc cctatgattt tacgttttgc ttccggatcg ttttcgccag
     2281 ccagcgctga caggaagcga tcttctgccg gtacgtgaac aatgttcaga ccaaagtgat
     2341 cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga
     2401 atacgcaagt caggttttta ccgatagcgc ggtgcagcag cattgcggtt acggaggaat
     2461 ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag
     2521 ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac
     2581 gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact
     2641 gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc
     2701 tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga
     2761 catcgagcag cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc
     2821 tgtcgtttac gacttcaacc tgcgcgtaac caaattcacg ttcgttagag gcttcaacgt
     2881 gaccgcccaa ctgcattgcc atggtctgca tgccatagca aatgccgaat accggtacgc
     2941 ctgcttcaaa gacatactgc ggcgcacgcg ggctgttttc ttcggtggtg ctttccgggc
     3001 cgccggaaag aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc
     3061 acgcccacag ttcgcagtaa acacccagtt cacgcacgcg gcgcgcaacc agttgagtgt
     3121 actgagaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg
     3181 cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga
     3241 gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg
     3301 gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc
     3361 gatagtacca cagccggtca gacccataca ggagcgcagg ccgcccatct gctggtgaat
     3421 gatctctttc aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt
     3481 gtcggcagcg ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag
     3541 ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg
     3601 agattcttca gtacccgcca gcatggaacc taccatcacc gcgcttgcgc cagcggcgat
     3661 agctttggcg atgtcgccgg agaagcgaat accaccatct gcgataaccg gaataccggt
     3721 gccttccagg gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt
     3781 aacgatacga gtcgtacaga tagaaccagg gccgataccg actttaaccg cgctgcaacc
     3841 agcttctgcc aaagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag
     3901 atccggatat ttagcacggg tttcacggat acgctgcaaa acgccttctg agtgaccgtg
     3961 ggaggagtcg atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc
     4021 gttacccgca cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca
     4081 ggcgttcggt ttacgttccg ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg
     4141 gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac
     4201 cacttcacgg gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac
     4261 gctaaccggc tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatacccac
     4321 cagttcgttt tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cagtcagttc
     4381 tttcacttcg cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga
     4441 ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg
     4501 gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt
     4561 atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct
     4621 gaggtcagca gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa
     4681 cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa
     4741 atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat
     4801 gcttgccatc cccgaagggc gggttactat tgactgaata acctgctgat ttagaatttg
     4861 atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc
     4921 aaacggttcg tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct
     4981 ctaatttcac acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc
     5041 aggtacgctg cgcgatgttc cgcaacagca accgccgggt gaccttccgt ccacagcatg
     5101 gacaacaagt tttagttcgc gccaatatta cgctctacga gccgcgcggt gactaccaga
     5161 taatcgttga gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc
     5221 tcaaagcgaa gttgcaggct gaaggtttgt tcgatcagca atacaaaaaa ccacttccct
     5281 cccctgcgca ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt
     5341 tgcatgtgtt aaaacgtcgc gatccttctc taccggtgat catctacccc accgccgttc
     5401 agggcgatga cgcaccgggg caaattgttc gcgccattga actggcgaat cagcgcaatg
     5461 agttcgacgt gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta
     5521 acgacgaacg cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg
     5581 ggcatgagac ggatgtgacc attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt
     5641 ctgccgccgc tgaagtagtg agccgtaatc agcaagagtt actgcgccag gtgcaatcga
     5701 cccgtcaacg gctggagatg gcgatggatt attatctcgc caaccgcacg cgtcgcttta
     5761 cgcagatcca tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa
     5821 ccatgcttga gcgcctgcaa aagcggatga gctttgcgct ggaaaatcaa cttaagcgta
     5881 ccgggcaaca gcagcagcgg ttaacacagc ggctgaatca gcaaaatcca cagccgaaga
     5941 ttcatcgcgc gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg
     6001 tacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa
     6061 gcccactgtc aacgctggcg cgtggataca gcgttactac tgctactgac ggcaatgtac
     6121 tgaaaaaagt gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct
     6181 ggatagaaag tgaagttaaa aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt
     6241 aagccagcac gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt
     6301 aatctactgc accgcaggct tttaacacct gcaacggttg atggcagtct ggacaaagag
     6361 ctttcatttc aataatttcc ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa
     6421 gcacatgctg acattgtgga caatgcagtt ccatatgaat tcctccggta gccatccctc
     6481 tacattcata gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat
     6541 gtgcttcatc agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttatt
     6601 cgcatacggg ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag
     6661 cgatttgcgg aagtagttca tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg
     6721 attaccgtga atcaccacaa tcggcgggtt ataaccaccg gcgtgggcat atttcagctt
     6781 cacacgacga ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg
     6841 cgtcagcata gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc
     6901 aaacaagtta ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc
     6961 gataaagccc agacggaagt ccagcgtttc tttcacctgc tctttcactt cctggctcag
     7021 gccatcccac ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag
     7081 cagcgagaga tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt
     7141 ggcgtcttca atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat
     7201 tttgccgcgt ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc
     7261 cattgggatg tagatgctgt cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc
     7321 ttcaccaaga atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc
     7381 cagtttgatc ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc
     7441 ttccgcttca aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag
     7501 atcttccatc cacggcagca gcacatgctc cagcagactt aatacgccac gaccgtgaga
     7561 cgcggcgatc gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc
     7621 gggatccaga ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag
     7681 atgtttggca atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag
     7741 tacgacgtcc gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc
     7801 gtcttctgtg ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc
     7861 cgcacgaccg tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg
     7921 ggtgcgagtt agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc
     7981 gaccacaggt accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata
     8041 ttcgcgtcgt tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa
     8101 cgacagagac gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag
     8161 tttgccgtca gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc
     8221 aacgaaacga ccatcttcga cgttaatcca gtgcagataa ccttcactgt cacctaccac
     8281 caggttgcca ttatacagca ccggagaagt cagcaggcga tgcaacagat cgctttgtgt
     8341 ccacagcgta acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag
     8401 atagatgcga ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat
     8461 aatctgacca ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac
     8521 aacgccgtta acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga
     8581 accggtcgcc tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc
     8641 gctgacgcga ccattatcgc cacccacgac ggccgcacca aaagccgttg ccggcgcaga
     8701 ctcgccacgc aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc
     8761 agcttcgttc agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct
     8821 gaccaccggg cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc
     8881 gctggtattc agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gcccaccaga
     8941 cacggtcaga ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc
     9001 caggctgaca gaccagattt ctttgccatc atccgcattc agcgctttta ctaaaccagc
     9061 gcggtccgct gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt
     9121 gccaatgccg ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac
     9181 ggttggcaat ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct
     9241 taaaagggta acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct
     9301 ctcagatgga caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat
     9361 cgcttttcac gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca
     9421 atgcttcacc acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atgctatcaa
     9481 gggttttcag cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat
     9541 ttatcacggc tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg
     9601 cagctttctc tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg
     9661 caccataagt atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt
     9721 tgccttcgct cactgcggta atcgcatttt gataggcaag agaagtggag cgtgcagaat
     9781 caacctgatg gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc
     9841 caacagccag tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt
     9901 tttcgttctc gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa
     9961 atgcgcggct acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac
    10021 tactgctgtg ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca
    10081 tttatcagca cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt
    10141 cacgcccggt aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc
    10201 agcacctgaa gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac
    10261 ggcctgtact aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc
    10321 acgaccgccc agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg
    10381 ggagccgaga ctgttagtca cccactcgaa aacggtacgg ttatagtaat ccagaccacg
    10441 caccagacgc tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc
    10501 ggcaaaatgc tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag
    10561 aagcgcctgc acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg
    10621 gcgtttgcag tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc
    10681 gcggtaattg gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc
    10741 ggaaataccc agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc
    10801 cggaccttgc agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt
    10861 ctgcggacgc tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta
    10921 cagaagacca tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt
    10981 caggctgtcg ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac
    11041 ttcaccaatc gcacgtttga atagcggggt ctgctctaca atcggcaaac ggatttcact
    11101 gtaaccgtag ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt
    11161 ttcgccaggc aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat
    11221 tctctttctg aatataaaaa tgaaccctca acgcttccct caatgtttgg ggagccatgc
    11281 gggttcaatc atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc
    11341 tgaacgtcaa ttcgacgcgc ttcatccagc tgactggctt tcgcacgaat gcgtgcttcc
    11401 agctggtcga tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagagg
    11461 ccgcttttct tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca
    11521 ttcaccacgc agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc
    11581 tccagcgcgt taaccgtacc gataacatca aattcctgac gcgaacaggt cgggcaggcg
    11641 atgaagttaa tccctcgcga acggatacgc agcgatttca aaatatcgaa accgactttg
    11701 atctcttcga ccggatcggc agccagcgat acgcgcagcg tgtcgccgat gccttcagac
    11761 agcagcagtc ctaaaccaac ggcggatttt actgccccgc tgcgtgcacc acccgcttcg
    11821 gtgatcccca gatgcagcgg ctgatcgatc tgttttgcca gcaaacgata agactcaaca
    11881 gcgaggaaga cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg
    11941 agatgatcaa catgacgcat ggcggattcc agcaatgcct gcggcgtcgg ttcgccatac
    12001 ttttcttgca gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt
    12061 ttatcgcgcg cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg
    12121 ttaatacgca gacaatcgac gccgtattcc gctactttca gcgcaatgcg atagtcgaag
    12181 tggatgtcag ccaccagcgg cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc
    12241 gcgtccatcg tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg
    12301 atttgattga ccgttgcttc gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg
    12361 atgggagcac catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctgcgt
    12421 tgaattggag cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg
    12481 gtgagtgttc ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca
    12541 gatcgacagg tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt
    12601 tgtacggtgc ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca
    12661 attttttacc ggtagcatca gtgacctcca gccagcaatc ggcagtaaag ttcatcacca
    12721 gcgcattcgg atcagccacc ggcgtggtca cgccagcctg atcggttggc aacggcgcag
    12781 caccatctgg cgttgttgct gccgttgatg ccggggtcgc ggcggtatca acatttgcct
    12841 gcgaaggcga aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacgg
    12901 caggtgtttg cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag
    12961 ccgggtctgt agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc
    13021 tcagttccgc cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt
    13081 cttgccacca ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg
    13141 tcatcagcca gccgtcacgt tttttgcggc gtttaccgag ggaaaaactc tgcatcggcg
    13201 caactttcgc agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg
    13261 gaatatgtac cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat
    13321 cggcgggtgc cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct
    13381 cggcaacggc ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg
    13441 tagtaagtgc ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt
    13501 aaatgagaat tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta
    13561 agttaaccgt tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca
    13621 ctaaggctaa cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg
    13681 ttaatattga ccagacaatt gcaacttaat acttcattca taaaagatta cgccacggta
    13741 cataaagtaa ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg
    13801 catccgttta cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca
    13861 ggcagcatcg atatcatcac cacgagtttt acgcacaata gtggtgaaac cgtagctcat
    13921 cagcactttt gagaaacggt cgatacggct gttcgaactg cgtccatacg gcgcgcccgg
    13981 gaacgggttc cacgggatca ggttgatctt acacggcgta tctttcagca gctccgccag
    14041 ttggtgcgcg tgttcagtgc cgtcgttaac gtggtcaagc atcacgtatt caatagtgac
    14101 tcggccctga ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc
    14161 gatattgtac tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg
    14221 cagggaaatt gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg ccggaactac
    14281 accggaagta gaaagcgtga cgcgacgttt agacaggcca aaaccgaaat catcaagcat
    14341 gatttccatc gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat
    14401 cactacgtta gtgattgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc
    14461 acgccacacc tggccgataa tttccgacac ccgcaggttg cggttaaagc cctgctgggc
    14521 ggtggaacag aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtggc
    14581 acggtcgtct tccgggatat acaccgtttc gacgcgctga tcgccaacgg cgatcgccca
    14641 tttaatggtg ccgtcggatg aacgctgttc ttcaaccact tccggtgcgc ggatctccgc
    14701 cacctctttc agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaagttgtc
    14761 gcagcaatag tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa
    14821 atctttaaaa aactcccgca tctgctgacg gttgagatcc agcaggttga tttttccatc
    14881 tttcgtggtg acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt
    14941 tattacacgt tatggcccct ggagggttga aaaaagaaac gccccggtga gcggctgctc
    15001 gtccgggggc gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat
    15061 ttacgaaatt attaacgggt gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt
    15121 tcgcgagcgg cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca
    15181 gcgtaatcag cgcgcagagt accagccagt gcgtttgccg gattggtcgc gcccagcaga
    15241 tcgcggtgac gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag
    15301 gtcatgaatt caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca
    15361 cgtgcctgtt caacggtcag gtgcagcatt ttggtgccaa caattttgaa ccctgcagct
    15421 tcaaagcgcg caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgatgatg
    15481 gaaaaagtac gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc
    15541 agcgtacgag atggcgcgga ttataatgag caacagggcc gttgactatt gatgaaggta
    15601 acattttttt aaaataaaat gagttttagc aacaatcagg gacaaaacag actattgcat
    15661 gacaaatttc actgtcgcga tttgccccac gtcatccatc accagcaatt gataatcacc
    15721 tttatccgtc aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag
    15781 aaaccaccag cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc
    15841 gcccggtaaa cgtttaataa tcgcgccatc gcgaacgcct gtcagctgca gcgggagctg
    15901 ggcatcgtga ccgtatggcg ggcaacttgt cgaggccggt ggtaagcgca cagcgcgacg
    15961 ttctgatgcg ggcagccagg gttccagcgg tagcggccag acatttatca tttcttgtcg
    16021 cgcttgcggg caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg
    16081 aatgccatta atgccttcct gctccggcag taatagagtt ggcggctgac tcccgtccag
    16141 cagccaggtt gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc
    16201 cggccagcag ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattcgc
    16261 actgcgcgac agtaagatat tattgacctg attcaacaat ggtacggcac tggcaaagcc
    16321 aaactgacca acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg
    16381 agcgttaacc ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc
    16441 cagtggggcg acgcgcggca aggcactatc cggcaagggt tgcgcttcat cagccataat
    16501 ccggcgaatg atccacgcag cccccgagga cattaaaggc cgttcaagca gcggatcgtc
    16561 aggctgtaag cgcaatttgc cggccttgcc gtggcgagca aacgcagtat acgctgccgc
    16621 catatcttcc agttttgcac cagcgccgcc gagaatgagt gaaagattgg gcgcagcacc
    16681 gttgggcaaa tataacggca atccaacatt gcgtaacttt gccgcaaacc ttttcggtcc
    16741 ataggcttcc agcacctgta cagcaggtaa attcagcgag cgcaccagcg cctcgctcat
    16801 gctgatcggg ccatgaaaac cgctatcaaa gttacctggt cgataatcac cggtgcgccg
    16861 ggggacgtct tgcagcagtg atgccggatg gatcaagcct tcatccagcg ccagaccata
    16921 aacaaacggt ttgagcaccg atcctggcga tcggatcgaa ttgaccatat caacatgacc
    16981 aaagcgtgaa tcatcgttga gatcaaccga tcccacccag ccgcgaacac gcatatcggt
    17041 atgatcaacc acgatcatcg ccagtgagct gcgcggtggc aaccgccctt tccagttttg
    17101 cgccagttct tccagacgtc gttgaagacc ggcatccaac gtagtagtga ttttgtcgct
    17161 tttgctttta ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg
    17221 ggccagccag atgggttctt cccttgactc ttttacctgc tcacgggacc acacaccttg
    17281 cacggccatc cgttcgagca ctttattacg cgcggcttcg gcacgctccg gccaacgatc
    17341 cgggcgaaga cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct
    17401 taaattcgca ggcgattttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt
    17461 accgccaaac ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca
    17521 ttccagttgc aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg
    17581 atcaagcaga cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga
    17641 agtgagatct tgccatgctg cgcgcgccac cgagaatgga ttcacccccg gatgcttcca
    17701 gaaccagcga tcttcataat tgatcagcgc ttcaaggtaa cgtggagaca catcttcgat
    17761 tgttaccgga taacgccaga tgccatcagc atcggcgaag cgccagagcg gcgtaccatc
    17821 ctgcgccacg accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc
    17881 tgctccccac gctgcaagaa aaaggagaaa tggcgcagct gccagcgtta tccagcagcc
    17941 gcgtttggtt aacaagcgaa gcatttacgg tcggacaatc agcagatctt cagccgcgcc
    18001 ggtcgcccgc cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt
    18061 tcccggcgtc accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc
    18121 aaccgccgcc acaaagcgat cgtcacggaa ctcaatgtgc ttaatgctcg cctgctgcat
    18181 ctggttcagt aagttttgca cttcgccacc actttgctcc aggctggcgc taccgttcgc
    18241 cagattctga ttttccagtt ccagacccgc aggcagcaga tccacgacta acgcatccgg
    18301 cacgctgtta ctggctttta cctgcaacca gaccagcacc agatcgccgc tacgtaacga
    18361 gtccagcgat ttgctcttac catcagtacc aagaatatga cgctcgattt gcagcacatt
    18421 gttcgccggt aaaggtgcgg attgcggata accgctggca tccatacgca accataacgg
    18481 ctgatcgcca ctgtttctca cctgcaaggt ggcaagttga tcgctattca gattgctgtt
    18541 tagcgttttc tcgcctgtca gcggctcagc tgagaaagag gtttgcgcct gccatttacc
    18601 cggtaaatcc tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag
    18661 ccagcgttca ccaaacgcct gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg
    18721 tagcagttta ttttcttcca gcaaggagag cattaacgcg ttgtcgcgca gtggactacc
    18781 gtaatcaccc agccatatcc gctcatcact atttctcggc gttttcagcg ccagcgcaat
    18841 cgcttcttca ccacgcgtcg catcacccat ggttttcagc gcaatgccaa gttgcagcag
    18901 cggtaaacca gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg
    18961 tgctttttgc tgacgggcca gcaccagcgc agcgtaagac tgaacggcga atttactggc
    19021 tttgagatta tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag
    19081 acgctcatta ccccggttaa tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg
    19141 gaccaggaaa tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca
    19201 cagcgcaaag ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac
    19261 cgatgcacgg cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc
    19321 gttggtataa agtgacggga acaggccgct ggcggtttgc tcaagacagc cgtacggata
    19381 cgcttttaac tctttgatat aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa
    19441 ttgcccttcc agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt
    19501 ctcaccaggc tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac
    19561 gccgattttc cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct
    19621 aatggtggcc tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag
    19681 cgtagtacgc acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag
    19741 caaaccactg gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat
    19801 atccagcgtc agacgcgagg tatcgccact cgccataaag cgcggcatgt tcagttcagc
    19861 aatcaccggt gcggcaacta tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca
    19921 ggcttgcgcc atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc
    19981 ttcgccctgt tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt
    20041 gaccggcggt ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc
    20101 cagacgcccc tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg
    20161 tttctgacca aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac
    20221 accactatca acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt
    20281 gctggcttta attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag
    20341 cgccagatcg aggcgacggt tttcatcgcc aagcggcaga tgcaacacac caaccgcgcg
    20401 ttttggcgtc gcggagcgag atttatcgcc aggacgtacc accagcgtac ttaaatacag
    20461 atcatgacga ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagccggaac
    20521 atcaatctct tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc
    20581 cgccgttggc gcggcgatat gcaacttaat ggtgtcgcca gggcgataac tggctttatc
    20641 cagtttcagc gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctgt tgtcctgcca
    20701 gctatagcca gcccagaaac gaacactact gaccgcttca ttcggcgctt tgacttccag
    20761 acgataagcg ccccactcta ccggaaaact gaccttaccg gtttcgtccg cgttcagatc
    20821 cagagtttgt tcattttcga tcagatcttt ttgatcaaac tgtgactgcc agccttcatc
    20881 ttctgaccag ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga
    20941 cacggctttt ttcacgcctt gtgcatcgct ataaacgatg tcaaaagcgg cgttactgcc
    21001 ttcatcaaca atgggctgtt tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcaa
    21061 ggcgaactgc ggacggagcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg
    21121 gcgcgtcacc gggcgaccgc ccgattccag caaactgccc tggaaaataa cctgcaatgg
    21181 ggaatgcgtt tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag
    21241 cgtcaactga acttcatcca gcgtgcggga aaggttttcg gcagctatat cgccgaattc
    21301 aaaaccaggt aaggctgaca cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa
    21361 agtattacca ttagcaggcg caccgtacag gtagtatccc accacggaga atttcacttc
    21421 atcattcggc gtaagcgggg ttttctcacc ggtcagattc agcgccatac gctctggcat
    21481 aaaatcttcg acgtggaaat cccacatccg gtactggtta tcgcccgtgt tggcgcgaat
    21541 atgccacata ccggttgccg cattgctatc gagtggccag gtaaagtggt agaggccatt
    21601 ctccggctga ctaacaacgc tcctgagtac ctgcccgtca ggtttaatca cgtctaactt
    21661 gatgggttga tcgggcaatg ctttaccgtc tgcatcgcgc agtaaaccat tgaggattac
    21721 cgtttcacct gggcgataaa gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc
    21781 tggcgcgcca gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag
    21841 cgttgtctga ccgtctttac gtgccagcag taatgccgca tttttatcat tttccagctg
    21901 cacatgcccc tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa
    21961 agagacttca attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag
    22021 acgattgtga taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg
    22081 attactgtaa tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg
    22141 aagcggtttg atatcgccca gcggcagcaa taatttttca cgggtgttac gtgcaggatt
    22201 gagatcaaac cgtccggtgt agaccagatc cgccatctgc agcagtttgt ctgactgcca
    22261 gttcgccagc gaattgcggt attcccattg gctaatgaat gctggcagag attctggctt
    22321 aacgcggaag aagttaacat cgacattatt aacgttgagc gccattaccg gcagcccttc
    22381 aacgactttg ccaggcagca gcgaaccacg gctggcaaaa ccgacgctag gttggatgtc
    22441 gcgggtagtt atagtttttt cgtaatcttt actgaaggtt gcgttgttga gtgctttgac
    22501 ctccttgcca atagtaacga tcaaatcacg tttcggttcg aggtggcgta aacgtagctc
    22561 tttaagatta tctgacagct cccaggcacc atccactttg ccgctttttt tatcgacgac
    22621 atgaataacg cgtgagaaat cctgatccgg gtcgagaggg atggagaacg tcagcaccag
    22681 cgtggctgca ccatcaagtt ggacttcaga gagatccagc aatgtcagca ccttaccggc
    22741 actctgttgg gccagttttt gtctctccgg cacggagagt tttgctgaac tcgcgttttc
    22801 tgaagaggcc gctttagtaa cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt
    22861 atcgttgttg tcgcaccctg ccagcgccag cattagcatg caggcggcta cgcgtaactt
    22921 tttcattttt catccctgcc acaatggccc gttagcaacg tcgaataatt attatgcgtg
    22981 agaatttacg ttatgtaatt cagtatcacg cccatacaat ccgaatttta gtgagaattg
    23041 tggctcagac agccaaatca gcctcttttg cttgtcgctt ttcactaaac atccgacaat
    23101 ttagcctaac cccggcaaaa atggagatgc ctatgtccac gacatggttt gtaggagccg
    23161 actggctcgc cgaacatatt gatgacccgg aaattcagat tatcgatgcc cgcatggcgt
    23221 cgcctggaca ggaggatcgt aacgttgctc aggagtatct gaatggacat attcccggcg
    23281 cagtgttttt tgatatcgaa gcgctttctg atcacacttc cccgcttccg cacatgctgc
    23341 cgcgcccgga aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag gataagcacc
    23401 tgattgtcta tgacgaaggt aatcttttct cagccccacg agcatggtgg atgctgcgca
    23461 cctttggtgt agagaaagtg tcgattctgg ggggtggact tgcaggctgg cagcgcgatg
    23521 atctgttgtt agaagaaggt gcagtagagc tgccggaagg agagtttaac gccgcgttta
    23581 atcctgaagc agtggtgaaa gtaaccgatg tgttattggc aagccatgaa aatacggcgc
    23641 aaattattga tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag
    23701 gtttacgtcg cggacatatt cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg
    23761 aaggcgaact aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc tgcgtcagct
    23821 acgacaaacc aattatcgtc agctgcggct ctggtgtaac ggcagccgtg gttttgttag
    23881 cactcgcgac gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg
    23941 gcgcgcgggc agatttaccg gttgagccag tgaaataagc tttatacagg caataaaaaa
    24001 ccgccgaatt tggcggtttt ttattactgg tctggttcgc ggcctttcca gcaggttgta
    24061 ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt
    24121 gtcaagcaaa agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg
    24181 ccgtctcgca agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg
    24241 cgtctcagcg cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg
    24301 catggaccgc acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct
    24361 gttttcccaa gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa
    24421 ccttcagagc agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc
    24481 cggttttgtt taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcacaata
    24541 tgttcggtaa taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt
    24601 ggttcatcgc caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg
    24661 atatcaccat cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta
    24721 attgaacaaa taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa
    24781 tcaatcattt gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc
    24841 aggatttcct ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc
    24901 ggcataaatt ctttaccggt tggcagtttg gcattaagga agagcgtttc gccaagtgtc
    24961 atctcaaaca atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt
    25021 tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag
    25081 tgctgtaaat caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc
    25141 acccagacgg tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt
    25201 gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt
    25261 cattcaacgt tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt
    25321 cacaatttgt tgaatttgca atactttgta ggccggataa ggcgtttacg ccgcatccgg
    25381 catattagtt tacgccgtta acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc
    25441 cgcagaccac tgttcaaccg gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca
    25501 gccttgctga tagttctcaa caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc
    25561 cggatacgcc gcgcttccgg tattgttcag ttcggcaaag ttagacggca gctggctgcg
    25621 gtggaattcc gccagcggca gacgccagaa tggttcgttc tcttgtgagg cactcgccag
    25681 caaacgaccg gcaagcgcat cgtcaaaact gaacagcgcg tgataatcat tacccagcgc
    25741 agttttcgcc gccccggtga gggtcgccgc atcaatgatc atttccggtt tctgcgcact
    25801 ggcatcaatc agaccatcgg caagcaccag acgcccttcg gcatcagtgt tcatcacttc
    25861 aactttttta ccgttgcgat aggtgatgat atcgcccagc ttgaacgcat tgccgctaat
    25921 caggttatcc gcacagcaga ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc
    25981 aaatgccagc gccccggtaa ccgttgccgc gccgcccatg tccgacttca tcgagtccat
    26041 aaacgcagtc tgtttgatgc tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag
    26101 gcacgcgtac actggcgctt ctttatcgcc agttgggttg taatccagcg ccagcaatac
    26161 cggagaacgt tctgaaccgc gtccgacggt gtgcagcccc atataacctt gctcacgcag
    26221 atcttcgcct ttggtgatcc gataagtcac acgatcgccc gcgacgttgc tgatcagatc
    26281 aacagcacgc tgtgccagtt gcgatggtcc caattcttct gccggtgcgt tgatggtgtc
    26341 acgcacccag tcgatgatca tcaggcggtt atccagttcc tggcgctggg catcgtccag
    26401 atccggccac tccactttac gcgtgccttt cggggctttg taaccttgcc agaatgccca
    26461 gcagcgatcc gcatcccagc cttcaccgct taactgaaca tgcttgatgc ccagaccgtc
    26521 aatcttgcgc gccgcacgct ggatcagccc cagatcgtct gccccgttca aatgcagggt
    26581 aatgccgtca ttattaatgc tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg
    26641 ggtagaaagg gtaatcttca tcgcttctgt cattttagtt atccttcttt gtaaaagggc
    26701 cgccagttgg cagccccagg atttttccgc taaatgattc gcgttgcagg aaggcggcaa
    26761 gtgagtgaag ccccaggagc atagataact atgtgactgg ggtgaacgag cgcagccaac
    26821 gcatctgcgg cgtgaagcat gacgcggaaa ttattcggcc tcgtccagcc agactaacaa
    26881 aatcgcttcg aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc
    26941 gcaaatccac tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc
    27001 atcgtacagt gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc
    27061 tgttaatgct cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg
    27121 tcggtgacgc gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc
    27181 agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg
    27241 cagtggcagg tggtgcaagc acaggatttt tcacaggcgt gttcaatctc gataccgtta
    27301 cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg
    27361 cagaggtcct gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg
    27421 gcctttcagc gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg
    27481 tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gagccacttc
    27541 actcaggtga gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc
    27601 atcagcagcc agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc
    27661 tgccagcata cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga
    27721 agcgatttcg ctatcggtca gaccgtaaga aggtttgacc tgaatagacg cctcaacgcc
    27781 ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac
    27841 gcgaatatgc gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag
    27901 tgagcggcag tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt
    27961 ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg
    28021 cggaatcact ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac
    28081 atcaagcagc agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc
    28141 cgcgccaata gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa
    28201 ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc
    28261 cagcacttca tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt
    28321 tcgttttacc agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca
    28381 gcccgcaacg ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggc
    28441 ggcatccagc agttcacgct gaacgcggtt atcgctacga tcaggaatgc ccgcctgctc
    28501 gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc
    28561 ggttgccagc acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc
    28621 gccaccgagg tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat
    28681 agccgcagcg gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc
    28741 gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc
    28801 atccagctcg cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc
    28861 agaaacgcgc accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc
    28921 gctggcctgg aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg
    28981 ccccatcagg cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg
    29041 cgcgtcataa cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg
    29101 acggccttca tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga
    29161 gttggttgtg cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa
    29221 accaggttca ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca
    29281 gtttttcttc gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca
    29341 cggtatccgc cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc
    29401 gggtatcaaa catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg
    29461 cctgttcgat ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt
    29521 cgcgcacagt atgctgctcg ctggtgagat caaaaccgtg taaagaaagc aaatattccg
    29581 cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta
    29641 cggcggcgag ttgttccgcc tggcttccgc tggcgaattt atcaggatga tactgacgtt
    29701 gtagatcctg aaaacgcagg ctcagcgcct gggtatcgag ttgataacgg gcaggcaagc
    29761 caaagagggt gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga
    29821 atcaggtaaa acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca
    29881 aacgtggaag ctttcgccgc aaccacactc atctttgacg ttcgggttgg tgaatttaaa
    29941 cccttcgttc aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct
    30001 tttgccatcg accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg
    30061 ttcgtcaaca aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag
    30121 acgcaggcca aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact
    30181 gtcgctcagt gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt
    30241 tatagtccgc aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca
    30301 ccggcggcag ttcaagttct tcagcaatat cggtattttt gatcgcctgc gcttcgtcga
    30361 gagacttccc tttcacccat tcggtgacca gggagctgga agcgatagca gaaccgcagc
    30421 cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact
    30481 tcatcacgtc gccacaggcc ggtgccccca ccatgccgct gccgacgttc tcgtcgttgt
    30541 tgtcaaagga acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag
    30601 ccattataaa ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca
    30661 gatccacgcc ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg
    30721 atttacgaac taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag
    30781 agaaacggat agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg
    30841 acggttcgag gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg
    30901 ccataatcag cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac
    30961 cgtgttccag gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac
    31021 ggttacgcag gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat
    31081 aggcctcgcc cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct
    31141 cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacgcg cggtttacga cgtacataca
    31201 gcgcaccgat acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt
    31261 tcaactggct caggtcgata ggcagtttgc ccacgctctg ggttgcatca acgtgataga
    31321 taatgccacg agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt
    31381 cgttatttac gtgcatgatg gaaacaagga tggtgtcggc acgcatcgct gcttcaagtt
    31441 ctttcaggtc gataatgccg ttacgctgcg gtgccaggta ggtgacttca aaaccttcgc
    31501 gctccagctg acggcaggta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt
    31561 gcttgccttt tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg
    31621 ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat
    31681 tacgggcgat atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg
    31741 ggttaccaaa ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt
    31801 ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc
    31861 cgtacatcac tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga
    31921 gtatttagca ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga
    31981 tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca
    32041 gcacttcctg gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac
    32101 ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc
    32161 ctttaccctg acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa
    32221 cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg
    32281 aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc
    32341 cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt
    32401 caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct
    32461 caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc
    32521 aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt
    32581 taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtccttc cagaccatgg
    32641 gcgggttgag ggcaattatt cgcattagcg ctggcctctt cccctcaccc taaccctctc
    32701 cccgaagggg cgaggggacc gtccgtgcat ttatctggct tagccctcag gccttattac
    32761 ctttattctg ctgctcaata gaagccagaa tcccgcgcag gatattcaat tcctggcttt
    32821 ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc ggatggtttt
    32881 cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa cgctccagat
    32941 catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccgttt tcctgagttg
    33001 ccagccaggc catgcgcact tcataggcaa taacctgaac cgccatcgcc aggttcagcg
    33061 agctgtattc cgggttagcc gcaatcgcga catgataatg gcatttctgc aactcttcat
    33121 tggtcaagcc gacgcgctcg cgaccaaaca ccagcgccac cggggtattt gccgcttcag
    33181 cgacgctttt caggccgcat tcgcgcgggt cgagcatcgg ccatggcagc gtgcgggaac
    33241 gtgcgctggt gcccaccacc aggctacaac cggctaacgc ttcgtcgagc gtatcgacga
    33301 tgtgagcatt accaatcaca tcgctggccc ctgctgccag ggcaatcgcc tgggagtcgg
    33361 gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc atggcacggg
    33421 caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga atattttgca
    33481 gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg aagacagatg
    33541 ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag tgagagagac
    33601 cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt aatttaattg
    33661 ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt aacgatttcg
    33721 tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt aaatcttacc
    33781 cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat caggatgttc
    33841 aatgggttat cgatccactg gatggtacta ccaactttat caaacgtctg ccgcacttcg
    33901 cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta tacgatccta
    33961 tgcgtaacga actgttcacc gcgactcgcg gtcagggcgc acagctgaac ggctaccgtc
    34021 tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc ttcccgttca
    34081 aagcaaaaca gtacgccact acctacatca acatcgtcgg caaactgttc aacgaatgtg
    34141 cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct gcgggtcgtg
    34201 ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc gagctgctgg
    34261 ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg tcataactat atgctgaccg
    34321 gtaacatcgt tgctggtaac ccgcgcgttg tgaaagccat gctggcgaac atgcgtgacg
    34381 agttaagcga cgctctgaag cgttaatgac tcaggcgggt gatatcactc acccgccctc
    34441 gcctttcagg cgctattccg aaatacttcc tcaccgcttt actttctttc gtcactctcc
    34501 caccatcttt ccccgattaa tggatgataa agaagtcaca atgaaaggtt atggcgctgc
    34561 cagtgaacaa acgcgttctc aaaattatgt ttattctctt tgttgttgcc ttctgcgttt
    34621 atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa atttacggtc
    34681 ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact cgtctgcaag
    34741 gctggtttat tccttcttcg acgggccctg ctgacaatgc catcgcaacc atcattcatg
    34801 ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg ttacccgagc
    34861 gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa ggcacgccgt
    34921 cccaggccgg attgctggac gatacgcaaa gcgccatcaa tgtggtgcgc catcgcagtg
    34981 atgtaaaccc acaacggctg gtgctgttcg ggcagagtat tggcggggcg aatattcttg
    35041 ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt gatcctcgac tccacatttg
    35101 cctcttatgc aaccatcgcc aaccagatga tccccggcag tggctactta cttgatgaga
    35161 gttacagcgg cgaaaattat atcgccagcg tcagcccgat cccgctttta ctcattcacg
    35221 gtaaagctga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc ctggcaaaag
    35281 agccaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt tccgatcgcc
    35341 acggcgatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg aatccgcaga
    35401 actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga catgctaatc
    35461 cactggtcag aacagtttaa gatgagaaaa attctgtgac gcttgccaac atttctgatg
    35521 attagcattc ccttcgtcat ttccctgagc aaactttagc tattcttatc aattatgctt
    35581 atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc ggctccccag
    35641 cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccat cacggcaact
    35701 ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag cgaaacagga
    35761 caggagatcc tgcgctatca tcaactcaca ctgacggctg gttatgacgg tagctaccgg
    35821 gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg acgtggtttc
    35881 cgtctgtgtc cgtcatttat taccagccaa ttcacccccg ccctgaagag tgaactgaag
    35941 cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt agtgaatctc
    36001 tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata ttcatttcct gtgtctgtat
    36061 ctgcaatatt gtttgctgca gcaccacgct ggaattacgc ctcagtttaa tccgctccaa
    36121 cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg acgccactgg
    36181 cagcgtcggg cgctccagcc tgtaccacct gatgagccac tgtttatggc actacttttt
    36241 tccatgttgc gggttcccga tccattgcgt gatgcgcatc agcgagacag acaattgcgt
    36301 cagtctatca aacatctggt aaaccatttt cgtgagctgg gaaatgttcg tttttatgat
    36361 gaacaggggt tatgcgatca gctttatacc cacctcgccc aggcgttaaa tcgcagtttg
    36421 tttgccatcg gtattgataa taccctgccg gaagagttcg ccagactgta cccacgcctg
    36481 gtgcgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt ccatctttct
    36541 gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct ggctaatgca ggaaaacgat
    36601 ctgcatgaaa aacagattat tctactgacc gggaatgata gcgagcgaga agcgcagatt
    36661 gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaagcatat gtcggtaaag
    36721 gcatttttgc agacaggcgc tccgcgtggc gcggcactga ttattgcgcc ttataccatg
    36781 ccattaccgc tcttttcacc accgctgatc tatacggacc tgacgctgac aacacatcaa
    36841 caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg ggcgcaaaaa
    36901 cattgccggc agcgccacca gcgccattac ccagaacacg ccgtggccca gatattgata
    36961 caggaaaccg gcgaaaacgg tcatgatagc gatactgccg cccatcgcga cggcagagta
    37021 aaccgcctgt aaacggatga cttcgctacc ctggcgagca gcaatataac gcatggcggc
    37081 caggtggcag accgtgaagg tgccgcaatg cagaatttgc accactatca accacggcaa
    37141 cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg agatcaacag
    37201 catatcgcgt gcactacaac ggcggaaaag tttattactc agcgcaaaga taatgacttc
    37261 cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc cagctgcctg
    37321 ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata aacaaacgca
    37381 ggccagaaag cgccagttct ggcgaaccag cgccaaccac gcagaccaac cggtgctctc
    37441 ctgctggcga cttgcccctt gtggctgaat cgtcggacgg atgagaaagc cgagcagcat
    37501 ggatgccacg cccaacgtca acagcgcgag gatcacccga taatcaaaca tagtgaccag
    37561 tttgcccgtc agcgccgagc caatgacaaa cgctaccgag ccccacagtc gcactttgcc
    37621 gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca acggtaccag
    37681 tggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta cgtgcgcccc
    37741 cgcccagaag gcgacagcaa agagaagtgt cagcagtgcc agcacgcgca aggcggaaat
    37801 caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagga aacgggcaac
    37861 cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc ctttaagcca
    37921 gacgctccag aaaggtagaa aaatgccgta actaaaaaag tatgtgaaat agccgagcgc
    37981 caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt tagtctgggg
    38041 ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt cccgatgtct
    38101 gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg caaaaataat
    38161 gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga aatcgcgtaa
    38221 tgccggcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag gcacatttcc
    38281 ggcaatggga cgaaaaacaa cctgcccggt attaaaatta ttcatataac cgggtatcaa
    38341 agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg ttgccacctg
    38401 gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg gcgcaagcga
    38461 accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg cggcggtgat
    38521 ctctttttca tgtgctaaag ggtgatcaac cggtaacaca accactaatg gttcgtcaaa
    38581 aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc cgacgtcgag
    38641 ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca gctcaatcaa
    38701 ggtgtctggc tgtctgagac gaaacatcgg taatactttt ggcagtaaat tcacttccgc
    38761 cgatggcaca aagccaatgg ttaattgtct gtcttcctga acaattttcc gcgcccgtaa
    38821 tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac attctcccgc
    38881 cgccgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga cacagttttc
    38941 aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt ccgccgcacg
    39001 ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta gttccatcac
    39061 cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt cacatattag
    39121 caaccaacca gcaacatcct tataacacaa aaatagaagg tcaatacatc ttatctttca
    39181 ggattaaaaa atatgaccac accctcagat ttgaacattt accaactgat tgatacccaa
    39241 aatggtcggg tcactccgcg tatttatacc gacccagata tttaccaact ggagcttgaa
    39301 cgtattttcg gccgttgctg gttatttctc gcccacgaaa gccagatccc aaaacctggt
    39361 gatttcttta acacctacat gggggaagat gcggttgtcg tagtgcgtca gaaagacggc
    39421 agcatcaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag ttatgcagat
    39481 tgcggcaaca ctcgcgcctt tacctgcccg tatcacggct ggtcttatgg cattaacggc
    39541 gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg taaatcccac
    39601 tgggggctaa acgaagttcc ttgtgtggag agttataaag gactgatttt tggcaactgg
    39661 gataccagcg caccgggcct gcgtgattac cttggtgaca ttacctggta tctggatggc
    39721 atgctggatc gtcgcgaagg cggtaccgaa attgtcggcg gcgtacaaaa gtgggtgatc
    39781 aactgtaact ggaaattccc ggcagagcag ttcgccagtg accaatatca tgctctgttc
    39841 agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga taagcgtctc
    39901 ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct gcaatttggt
    39961 caggacggtc acggtagcgg tttcttcttt actgaaaaac cggatgctaa tgtctgggtc
    40021 gatggcgcgg tttccagcta ttaccgcgaa acctatgccg aagcagaaca acgtttaggt
    40081 gaagttcgcg ccctgcgcct ggcgggtcat aacaatattt tccccacgct ttcatggcta
    40141 aacggcactg ccacactccg cgtctggcat ccgcgcggcc ctgatcaagt tgaagtgtgg
    40201 gcgttctgta ttactgacaa agccgcctcc gatgaagtta aagccgcttt tgaaaacagc
    40261 gccactcgtg cttttggtcc tgctggtttt ctcgagcagg atgactcgga gaactggtgt
    40321 gaaatccaga aattgctgaa aggtcaccgc gcccgcaaca gcaaactgtg tctggaaatg
    40381 gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg gcattactaa ctatatcttt
    40441 tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag tagcgaaagc
    40501 tggcaggaag tgctcgataa aaccgccgct taccagcagg aggtgatgaa atgagtgcgc
    40561 aagtttcaca ggagttacat caccgcatta gccagtttct ctttcacgaa gccagcttac
    40621 tggacgactg gaaatttcgt gactggctgg cacagctcga cgaagagatt cgttacacca
    40681 tgcgcaccac ggttaacgcg caaacgcgcg accgccgcaa aggcgttcag ccgccgacaa
    40741 cctggatttt taatgacacc aaagaccagc tggagcggcg aatcgcccgt ctggaaacgg
    40801 gcatggcctg ggcagaagag ccgccgtcac gcactcgtca cttaatcagc aactgccagg
    40861 taagcgaaac cgacatccca aacgtatttg ccgtgagggt gaattatcta ctttatcggg
    40921 cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa gttcgccgtc
    40981 tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa gcggtgatca
    41041 cttcccataa cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc ccgttgcgga
    41101 tgtgccggag ggtgaggctc tccggataga tacctcgccc gtcatcgcct tgttcaacgt
    41161 gggcggcgag ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt cgatgtcaga
    41221 agggtatctg gaagatgacg ccacggtgga gtgcccgcta cacgccgcca gtttttgcct
    41281 gaaaacgggg aaagcgttat gcctgcccgc caccgatccg ctcaccactt atccagtaca
    41341 cgttgaaggt ggtgacattt tcatcgactt accggaggcg cagccatgag cgatctgcat
    41401 aacgagtcca tttttattac cggcggcgga tcgggattag ggctggcgct ggtcgagcga
    41461 tttatcgaag aaggcgcgca ggttgccacg ctggaactgt cggcggcaaa agtcgccagt
    41521 ctgcgtcagc gatttggcga acatattctg gcggtggaag gtaacgtaac ctgttatgcc
    41581 gattatcaac gcgcggtcga tcagatcctg actcgttccg gcaagctgga ttgttttatc
    41641 ggcaatgcag gcatctggga tcacaatgcc tcactggtta atactcccgc agagacgctc
    41701 gaaaccggct tccacgagct gtttaacgtc aacgtactcg gttacctgct gggcgcaaaa
    41761 gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga ttttcacact gtcaaatgcc
    41821 gcctggtatc ctggcggcgg tggcccgctg tacaccgcca gtaaacatgc cgcaaccgga
    41881 cttattcgcc aactggctta tgaactggca ccgaaagtgc gggtgaatgg cgtcggcccg
    41941 tgtggtatgg ccagcgacct gcgcggccca caggcgctcg ggcaaagtga aacctcgata
    42001 atgcagtctc tgacgccgga gaaaattgcc gccattttac cgctgcaatt tttcccgcaa
    42061 ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa tcgcgcatta
    42121 agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg ccacgtagcg
    42181 gctgggctgg atctttaagg aagcacgatg aaagaaaaaa cgatcattat tgtcggtggc
    42241 gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc aagggttcac cggtgagctg
    42301 catctgtttt ccgatgagcg acatcttcct tatgaacgac ctccactctc gaaatccatg
    42361 ttgctggaag attccccgca gttacagcag gtgttacccg ctaactggtg gcaggaaaac
    42421 aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac acgagagtta
    42481 gtgttaacca acggcgaaag ctggcactgg gatcagcttt ttatagcaac cggcgcggca
    42541 gctcgaccgc tgccgttgct tgatgcactg ggagaacgct gctttaccct acgccatgcc
    42601 ggtgatgccg ccagactgcg agaagttctg cagcccgaac ggtcagtcgt gattatcggt
    42661 gccggaacta ttggtctgga actggctgcc agcgccacgc agcgcagatg taaggtgaca
    42721 gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac caccgcccgt gcaacgctat
    42781 cttttacagc gccatcagca ggctggtgtg cgcattctgc tcaataatgc cattgaacat
    42841 gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg gcgaaacgct tcaggccgat
    42901 gtggtgattt acggtattgg tatcagcgcc aacgagcaac tggctcgcga ggccaacctt
    42961 gatactgcca atggcattgt cattgatgag gcttgccgca cctgcgatcc cgcgatcttt
    43021 gccggtggcg atgtggcaat cactcgtctt gataatggtg cactacaccg ctgcgaaagc
    43081 tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg caatgttggg gctaccgctt
    43141 ccgcgactgc cgccgccgtg gttctggagc gatcagtaca gtaataactt acagtttatt
    43201 ggcgatatgc gtggtgatga ctggctttgt cgtggcaacc cggaaactca gaaggcgatt
    43261 tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtaa cgctgaatca gggacgtgag
    43321 attcgctcaa tccgcaaatg gatccagagc ggcaaaacgt ttgatgcgaa acagctgaca
    43381 gatgagaaca tcgcgcttaa atcactgtaa ccaggataat tagcgaatat ctcaacgcct
    43441 ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat cacattatct tgcaaaggga
    43501 ttggttatga acacactacg ttattttgat tttggagctg cccgccccgt tttgttatta
    43561 attgcccgta tcgccgtggt cttaattttc attatttttg gttttcccaa aatgatgggc
    43621 tttgacggta cggtccaata tatggcctcg ttgggcgcgc caatgccgat gctggcagcg
    43681 attattgcgg tagttatgga agtgcccgcc gcgatattaa tcgtgcttgg ctttttcacc
    43741 cgtccgctgg cggtgctgtt tattttctac acgctgggta cggcggtgat tggtcaccat
    43801 tactgggata tgaccggcga tgcggttggg ccaaatatga ttaatttctg gaagaatgtc
    43861 agtatcgctg gcgcgttctt gctattggca attaccgggc cgggggcaat ttctctcgat
    43921 cggcgttagg aaagatgccg gatgcggcgt gaacgcctta tccggcattt tataaattac
    43981 agccactcta cccgcaacga catctctgag gttgttgatt cccctggtgc tagcgcaatg
    44041 agatcacccc cttccgggcg atgatgatca tccggcgcat ggctcatcgg ttccagacag
    44101 aagaaatcaa acgcatatcc tttatcaaac gcagggtcgg aaacaaagat gaaataacac
    44161 ggtgcaggtg gcgtcgtttc catgatgatg gcataacctt cctgcggctg ctcgatgcgg
    44221 gcctgaccat tccatccggc aaaaccattg ttcacccact ggcgcggcaa cggcgcgagc
    44281 tggttaaaat ccagttcctg cggtagctgc tcgcaaaact cacccgccag ccactgctcc
    44341 cgttccagcc agtaaccgct cgcctgcgcc tgaatccgcg tttgtggtga caacggaaaa
    44401 taaggatgcc agccggtacc aaatggcagc gtctctgccc cttgattggt gacagagagc
    44461 gtcaccgtca gcgtatccgc cgttaaatga aacgcctgac ttacccgata gtgatagaca
    44521 ccgctgcgat gttcatacac cagacacaaa ctatcatcgc tgtgcgagac acattgccac
    44581 tcgcccagcc agccatcgcc gtgtagatag tgcgcatccc actcaacatt cggttgcagt
    44641 tgatactcac gcccctgcca gacaaaacgg ttgccgctca cccggtttgc aaatggcacc
    44701 agcggaaaac aggaggcatc ggttgccaca ccgctttttt tatcaggacg taagagcggc
    44761 gtcgtatcgc gccagaagcc ttcgattacg ccgccctggt cagaaacgtc cagctttagc
    44821 gacccgtggg ataaggtata gatggtcatc cgctaactcc ttaatccggg aagttaatca
    44881 caactttccc gcatttgccg ctcgccatca gcgcataggc atcacctgcc tgttccagcg
    44941 agaagcgatg ggtaatggcg ttacgcggcc acagtttcca gtccgttaaa tcgtgggcgc
    45001 atttttccat atggaacaga ctggtcaccc aggagccaat aatccgccgt tgatggtgca
    45061 tcagatcggc gctgacctcg aattccactt ttccggtttc accaatgtaa accccccgcc
    45121 cccagtcagc agtggattgc agtgccagca agcgacctgc ggcattaccg gaacaatcga
    45181 gcgcaacatc cgcgccaccg tgggtaagtt cagcaattat ctgcggcaga ccttcggtgg
    45241 tcgctaaata gccgtgatcc atcaccccta actgttttgc catcgccaga cgttccggca
    45301 gcatatcaac gccgatgatc cgttttgcac cgcgaccttt cgccagcatc atcgccatca
    45361 tgccgaccgg ccccagaccg accaccagca cgttatcgct gccggaaact tcgccgcgca
    45421 aaatcccttc ataggccgtg ccaacgccgc aactgataaa cgcaccatct tcgtagctca
    45481 gcgcatccgg caggaggatc agatcttttt cttccgccag cagatattcg gcatgaccgc
    45541 cgtcacgctg ccagccgtaa gccgcttttc ctttgccagt acaagagata ggaaagccgc
    45601 ggcggcagtt cgggcaaaaa ccacagccag aaatgtgata caccagcacg cgatcaccct
    45661 ctttaaaatg gcgacagcct tgccccatcg ccacaatctg cccgcacggt tcatgaccgt
    45721 tgataaatcc ctggtataac ggtttatcgg gtgccgccgc cgtggcacgg tgttgatgat
    45781 agatatagtg gacatcgctt ccgcaaatcc cggaggattt cattttgatc agtacctggt
    45841 taatccccgg cgtcggcacc gcaacttccc gcagatcgac ggttgaattt cctggtaaat
    45901 aagctgccag catcgttttc ataaatccct cattaacaat acgattaatt ttcatccctg
    45961 ccgcacccgc gccaggggcg ttaaggttta gcgtttcgct ttactgcttc gctgggtcag
    46021 caagatattc gccagcaccg ccaccacgat gatgacgccg cgtaccacct gctggaaaaa
    46081 ggagttaata ccgagcagca ccagaccgtt accgattagc gtaatcacca gcacaccaag
    46141 caatgtaccg aacagggaac cgcgaccgcc ggaaagtgcc gtaccgccga ccacgaccgc
    46201 ggcgatgacg tcaaactcca gaccgtttgc ggcacctgcg ttaccggaac cgaggcgtgc
    46261 cgccagcaaa atgccggtca ccgccgctaa taatcccgaa agggtaaaga taagaatgcg
    46321 cactcgacga acgttaatac cgcacaactg cgccgccgtg gcattaccgc caacggcaaa
    46381 aaccgagcgc ccgaaggcgg tttttcggct aatgaacaca aacagcgcaa acaacaccat
    46441 catgatcagc gcggatactg gcacaccgag aaattgtccg cccagccagt ccagcacctc
    46501 gttttcgtca atcggcactg gcagtgcgtt ggtcataaac agccccattc cgcgcagggc
    46561 gctccacagc cccagtgtgg caacgaaact gggcacgtta aacacgccgc gcagcacccc
    46621 ggcgagcgtt cccatcagcg cgcctaacag caacaccagc agacaagcaa tcgccaacgg
    46681 aacttcaaat tgcagcaaaa atgccaggca caccgagaca aaagccacca tcggtccaac
    46741 gctgacatca atttcaccgg agataataat cagcgtcatt gcccaggcag cgatcccgat
    46801 ggtggcagca tcgcgaagca cgttcatctg gttattcaat gagataaagc caggcgcatt
    46861 cagggagaag accagataaa gaatggcgat taccaccagc aaaccgatat cattaatatg
    46921 gcgactgaca aattgtttga gcgagacgct cttgccctgc ggcaatgata atgacgaagc
    46981 agacatcttc aggttcctcg gtaaaattca tcagtgcaca gacagaatgg cggacatcag
    47041 ctcatccaca ttgaccggcg agtgaaactc ctgcgagaac gtaccgtgct gtaacaacag
    47101 gatgcggtcg cacaccagcg gcaactcctc cacttcactg gagataaaca ccacgctttt
    47161 tccttcggca gccagctcac ggacaatacg gtaaatctgc tgtttggctt cgatatcgac
    47221 gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg gcatagaccc aacgaccgat
    47281 caccactttt tgctgattgc caccagaaag cgtgccgatg ggcgtttcgc tactggcggc
    47341 cttgaccgtc atccgctgca tcacctcttc ggtcaggcgg cggatggtgg accattgcag
    47401 cactccgttg gcgctgattt tttgccgatt ggtcagcact gtattttcgt caacgcccaa
    47461 ccagggaatg atccccgctt ctttgcggtt ttctggcgta tagccaatgc cgcgtttcag
    47521 catgtcgccg taatcggggc gcgtgatttt ctcgccgttg ataacaattt cgccctgttc
    47581 atacgtctcc agcccaacaa tcgccttcag caattcactg cgccctgccc ccagcaggcc
    47641 agcaatgcca agcacttcgc cgcgacgtaa cgtaaagctg atatcctcca gcttgggctt
    47701 atggcgtaac gcacggactt ccagtacggc ctgatccaca atttcctgag gggcaaccag
    47761 cgcaatatca acgtgatcac gccctagcat tagcgacaca atatgatgcg tggaggtgtt
    47821 ttcgagcatc acatcgcccg ccacctgacc atcgcgcata acggtggcac aggaggcaat
    47881 gcggcggatt tcttccatcc ggtggctgac ataaatcacc gccacgccca gtgccgacat
    47941 ctttttcacc gcactgatca ccagttcaac ttctgcactg gcaagcgagc tggtgggttc
    48001 atcaagaatg accacgcgcg gctcgccctt catcacccgt gcaatttcca ccagttgctt
    48061 ttgcgccggg cttagcgttg aaacaagttg ttcaggacta acgtcaacgc ccagcgcctg
    48121 taagcaacgt tgggcatcct gcgccatttg caggtaatca atcatgccgt tgcggcgagg
    48181 ccattgacca aggcagaggt tttctgccac cgtcagccct tccaccagac ttaattcctg
    48241 ataaaccgca cgaaccccca gttcagcggc acggcgagtc aacgtagctt cgtcaccttc
    48301 cagtcgcgtc tcgccaatcc agatatcacc gctatccggg cgttcgctgc cggtaagcat
    48361 tcgaatgaga gtcgatttgc ccgcgccgtt tttacctaac agcgcacgaa cttcgccttt
    48421 attgagcgtg aagttaacgt tatccagcgc aacgacgccg gggtaacgct tatttcctgc
    48481 caccactttt gctaccggga ctgcctctgt tgccgtgaac atagggacct ctgcgaatca
    48541 gcgattaggg cagaccatca acgtgcgttg ccagccactg tttaccatct tccgttttgg
    48601 tatagagatc gattggcacc tgaatcactt tttcaccgtc ggcttgttta ttgataacct
    48661 tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc actaccgctt
    48721 tcagcacctg attgttttcc agctcctgag caatttcggt tgtcatatcc gaaccgaaaa
    48781 cagcaatttt tccggcctga ttttgattac gtaccgcttt taccgcgccg agtgtcgcac
    48841 cgcccgactc ccccataatg gcgttgagat ccggcgtgga gataatcagt ttttcaccaa
    48901 cagaaatcgc tttatctaaa acagtccctt cctgattagc gacaatttgc gcgccgggaa
    48961 cacgggcttt taatacttct tcaaatcctt tacgtcgctg cacacaaact tcaaaggctt
    49021 cgcaattgat gacagcaatt ttcggctggt caattttgtt ggcaataaaa taatcggcgg
    49081 cagcattacc cagtttttta ccaaattcca gcggatcgcc gaccagatac gccgagacat
    49141 atttatcgac acccttttga ttaatacagg tgttgtagca aatcaccgga atacccgctc
    49201 cactggcgcg acgaacggta cgactactgc cattttcaga cactgccgat aaaataatgg
    49261 catcgacatt acgcgccacg agggtatcaa caaaggtact ttctttcgaa atatcaccct
    49321 gggcgttagt ttcaattaac tgtacctgta ctgaagaatc ttttgccgca tcctgaacgc
    49381 cctgacgcac tccagcgtaa tatccctgag tatcaaggta tattgcgcca atggtcattt
    49441 ctttttccgc agccctggca aatagtgcgc tacctaatag cgtagccatt aataatagat
    49501 tacgggttgt tctgattttt gtaggcatag agcctcctgt agggttttta ttaacaacgg
    49561 cttattctaa ttattttgtg atgagcggca gcgcgtgccg ctcaaatatt acagttaata
    49621 gggtttagtg aacgtagtta aaaataaaag gcatcacggt ggtgaataat gccgctttcg
    49681 gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc gcaacctccc
    49741 cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta
    49801 aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc gcctcaatac
    49861 tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc tgtgcggttt
    49921 gctgttcacc cagcagtctc agcgccattc cttgccagaa gagataatca accggctgat
    49981 cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac
    50041 gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag aaccagatgt
    50101 cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg
    50161 catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc caggcgcgta
    50221 ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc cacgggtgga
    50281 atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga tgccacaaat
    50341 tgagcagttc tgcggtcatg tcgtcgcgtt tcaggta
//
LOCUS       DAJSKJ010000009        47709 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000009 DAJSKJ010000000
VERSION     DAJSKJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.009"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            135..1352
                     /gene="alaA"
                     /locus_tag="ON345_000439"
     CDS             135..1352
                     /gene="alaA"
                     /locus_tag="ON345_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR3070551.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1436..2035
                     /gene="yfbR"
                     /locus_tag="ON345_000440"
     CDS             1436..2035
                     /gene="yfbR"
                     /locus_tag="ON345_000440"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR3070552.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2094..3926)
                     /locus_tag="ON345_000441"
     CDS             complement(2094..3926)
                     /locus_tag="ON345_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR3070553.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4013..4663)
                     /gene="hxpA"
                     /locus_tag="ON345_000442"
     CDS             complement(4013..4663)
                     /gene="hxpA"
                     /locus_tag="ON345_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR3070554.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4674..5168)
                     /locus_tag="ON345_000443"
     CDS             complement(4674..5168)
                     /locus_tag="ON345_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR3070555.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5251..5706)
                     /locus_tag="ON345_000444"
     CDS             complement(5251..5706)
                     /locus_tag="ON345_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR3070556.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6044..7246
                     /gene="ackA"
                     /locus_tag="ON345_000445"
     CDS             6044..7246
                     /gene="ackA"
                     /locus_tag="ON345_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR3070557.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7321..9465
                     /gene="pta"
                     /locus_tag="ON345_000446"
     CDS             7321..9465
                     /gene="pta"
                     /locus_tag="ON345_000446"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR3070558.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9655..11175
                     /gene="yfcC"
                     /locus_tag="ON345_000447"
     CDS             9655..11175
                     /gene="yfcC"
                     /locus_tag="ON345_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3070559.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11208..11750)
                     /gene="yfcD"
                     /locus_tag="ON345_000448"
     CDS             complement(11208..11750)
                     /gene="yfcD"
                     /locus_tag="ON345_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR3070560.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11808..12359)
                     /gene="yfcE"
                     /locus_tag="ON345_000449"
     CDS             complement(11808..12359)
                     /gene="yfcE"
                     /locus_tag="ON345_000449"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR3070561.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12415..13059)
                     /gene="yfcF"
                     /locus_tag="ON345_000450"
     CDS             complement(12415..13059)
                     /gene="yfcF"
                     /locus_tag="ON345_000450"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR3070562.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13195..13842
                     /gene="yfcG"
                     /locus_tag="ON345_000451"
     CDS             13195..13842
                     /gene="yfcG"
                     /locus_tag="ON345_000451"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR3070563.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVGLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13899..14261
                     /gene="folX"
                     /locus_tag="ON345_000452"
     CDS             13899..14261
                     /gene="folX"
                     /locus_tag="ON345_000452"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR3070564.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14282..15175
                     /locus_tag="ON345_000453"
     CDS             14282..15175
                     /locus_tag="ON345_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR3070565.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15223..16113)
                     /gene="rpnB"
                     /locus_tag="ON345_000454"
     CDS             complement(15223..16113)
                     /gene="rpnB"
                     /locus_tag="ON345_000454"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR3070566.1"
                     /translation="MTISTTSTPHDVVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16310..17083)
                     /gene="hisP"
                     /locus_tag="ON345_000455"
     CDS             complement(16310..17083)
                     /gene="hisP"
                     /locus_tag="ON345_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR3070567.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17091..17807)
                     /locus_tag="ON345_000456"
     CDS             complement(17091..17807)
                     /locus_tag="ON345_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3070568.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17804..18490)
                     /gene="hisQ"
                     /locus_tag="ON345_000457"
     CDS             complement(17804..18490)
                     /gene="hisQ"
                     /locus_tag="ON345_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR3070569.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18580..19362)
                     /gene="hisJ"
                     /locus_tag="ON345_000458"
     CDS             complement(18580..19362)
                     /gene="hisJ"
                     /locus_tag="ON345_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR3070570.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19583..20365)
                     /gene="argT"
                     /locus_tag="ON345_000459"
     CDS             complement(19583..20365)
                     /gene="argT"
                     /locus_tag="ON345_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR3070571.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20631..21200)
                     /gene="ubiX"
                     /locus_tag="ON345_000460"
     CDS             complement(20631..21200)
                     /gene="ubiX"
                     /locus_tag="ON345_000460"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR3070572.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21295..22812)
                     /gene="purF"
                     /locus_tag="ON345_000461"
     CDS             complement(21295..22812)
                     /gene="purF"
                     /locus_tag="ON345_000461"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR3070573.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGSDGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22849..23337)
                     /gene="cvpA"
                     /locus_tag="ON345_000462"
     CDS             complement(22849..23337)
                     /gene="cvpA"
                     /locus_tag="ON345_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR3070574.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23596..24258)
                     /gene="dedD"
                     /locus_tag="ON345_000463"
     CDS             complement(23596..24258)
                     /gene="dedD"
                     /locus_tag="ON345_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR3070575.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24248..25516)
                     /gene="folC"
                     /locus_tag="ON345_000464"
     CDS             complement(24248..25516)
                     /gene="folC"
                     /locus_tag="ON345_000464"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR3070576.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25586..26500)
                     /gene="accD"
                     /locus_tag="ON345_000465"
     CDS             complement(25586..26500)
                     /gene="accD"
                     /locus_tag="ON345_000465"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR3070577.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26656..27315)
                     /locus_tag="ON345_000466"
     CDS             complement(26656..27315)
                     /locus_tag="ON345_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR3070578.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27398..28210)
                     /gene="truA"
                     /locus_tag="ON345_000467"
     CDS             complement(27398..28210)
                     /gene="truA"
                     /locus_tag="ON345_000467"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3070579.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28210..29223)
                     /locus_tag="ON345_000468"
     CDS             complement(28210..29223)
                     /locus_tag="ON345_000468"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3070580.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29289..30425)
                     /gene="pdxB"
                     /locus_tag="ON345_000469"
     CDS             complement(29289..30425)
                     /gene="pdxB"
                     /locus_tag="ON345_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR3070581.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNATAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30524..31519
                     /gene="flk"
                     /locus_tag="ON345_000470"
     CDS             30524..31519
                     /gene="flk"
                     /locus_tag="ON345_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR3070582.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31516..32694)
                     /locus_tag="ON345_000471"
     CDS             complement(31516..32694)
                     /locus_tag="ON345_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HCR3070583.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33003..34223)
                     /gene="fabB"
                     /locus_tag="ON345_000472"
     CDS             complement(33003..34223)
                     /gene="fabB"
                     /locus_tag="ON345_000472"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR3070584.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34382..36388
                     /gene="mnmC"
                     /locus_tag="ON345_000473"
     CDS             34382..36388
                     /gene="mnmC"
                     /locus_tag="ON345_000473"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR3070585.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPKHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNHTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASMLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36509..36787)
                     /locus_tag="ON345_000474"
     CDS             complement(36509..36787)
                     /locus_tag="ON345_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR3070586.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36821..37369)
                     /gene="epmC"
                     /locus_tag="ON345_000475"
     CDS             complement(36821..37369)
                     /gene="epmC"
                     /locus_tag="ON345_000475"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR3070587.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37369..38178)
                     /locus_tag="ON345_000476"
     CDS             complement(37369..38178)
                     /locus_tag="ON345_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3070588.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38178..39002)
                     /gene="mepA"
                     /locus_tag="ON345_000477"
     CDS             complement(38178..39002)
                     /gene="mepA"
                     /locus_tag="ON345_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR3070589.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39006..40091)
                     /gene="aroC"
                     /locus_tag="ON345_000478"
     CDS             complement(39006..40091)
                     /gene="aroC"
                     /locus_tag="ON345_000478"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR3070590.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40126..41058)
                     /gene="prmB"
                     /locus_tag="ON345_000479"
     CDS             complement(40126..41058)
                     /gene="prmB"
                     /locus_tag="ON345_000479"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3070591.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELLNNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41224..41775
                     /gene="smrB"
                     /locus_tag="ON345_000480"
     CDS             41224..41775
                     /gene="smrB"
                     /locus_tag="ON345_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR3070592.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASYYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41897..42769)
                     /locus_tag="ON345_000481"
     CDS             complement(41897..42769)
                     /locus_tag="ON345_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR3070593.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42756..43280)
                     /locus_tag="ON345_000482"
     CDS             complement(42756..43280)
                     /locus_tag="ON345_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3070594.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43277..43747)
                     /locus_tag="ON345_000483"
     CDS             complement(43277..43747)
                     /locus_tag="ON345_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3070595.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQAIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43744..44292)
                     /locus_tag="ON345_000484"
     CDS             complement(43744..44292)
                     /locus_tag="ON345_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3070596.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44267..45019)
                     /locus_tag="ON345_000485"
     CDS             complement(44267..45019)
                     /locus_tag="ON345_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR3070597.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45039..47681)
                     /gene="yfcU"
                     /locus_tag="ON345_000486"
     CDS             complement(45039..47681)
                     /gene="yfcU"
                     /locus_tag="ON345_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR3070598.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTRNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
BASE COUNT        11776 a        12887 c        11833 g        11213 t
ORIGIN      
        1 cagcacaatc gagccttctc ctctggcaaa atcttattct gcagaccttc aaaacaccgt
       61 cctgggggag tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa
      121 aaaggtcaag attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg
      181 acatccgtgg tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac
      241 tgaaactgaa catcggcaac ccagccccat tcggttttga cgcgccagat gaaatcctcg
      301 ttgacgtgat acgcaacctg cctaccgctc aagggtattg cgattccaaa gggctttact
      361 ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg
      421 aagatattta catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc
      481 tgaacagcgg ggacgaaatg ttggttcctg caccagatta cccactctgg accgcggcgg
      541 tttcgctttc cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc
      601 cggacctcga tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc gttattatca
      661 acccaaataa cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga
      721 ttgcacgtca gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctctacg
      781 acgatgctga gcatcactca attgcgccac tggcacctga cctgctgacc attaccttta
      841 acggactgtc gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg
      901 ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc
      961 tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca
     1021 tcagtgaatt tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac
     1081 tgatcaacga tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc
     1141 cgaaaatcga cgccaaacgc tttaacattc acgacgatca gaaaatggtg ctggatttcc
     1201 tgttgcagga aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc
     1261 acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg
     1321 cgcgtttcct ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc
     1381 cggcagtgtt atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa
     1441 acagagccat ttttttgccc atctctcccg cttgaaactc attaaccgct ggccgctcat
     1501 gcgcaacgtg cggacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca
     1561 tgcgctggca gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc
     1621 tttactggcg atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt
     1681 gaaatacttc aattcgcaaa tcgctcagga gtacaaggct attgaaaaaa tcgctcagca
     1741 aaaactggtc gatatggttc cggaagagct gcgggatatc tttgcgccgt taattgacga
     1801 gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata
     1861 tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg
     1921 actggaagcg acgcttgaag cgcgtcgcag ccaggagatg gactacttca tggaagtatt
     1981 tgttcccagc ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc
     2041 cggagtctgc gtcgcatcag gcaataagcg ccggatgcga catcaggctc ttgtcaaaac
     2101 ggaaacagca tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc
     2161 cccaacttca caaagtcact gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca
     2221 ggtgaagaaa ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcgaat
     2281 ggataaggcg acacccccat cgttttggca gcagccagcg caatcggagc catcaacacc
     2341 gccgtcgcgg tattagagat aaatagccca ataaccgccg acaagacaaa caaacacccc
     2401 agcatcatat gtggcccgta accaccgcca atgtccatca gccctttcac cgccagcgcg
     2461 acacctcccg ttttctgtaa tgccacagca aacggcatca tcccaacgat caaaataatg
     2521 ctcggccagt gaatggattt ataggcgctt tcagcatcta tacagcggaa tttccccatc
     2581 agcaggcagg cgatgatagc ggcaacagga ttaggaattt catctgtcag cattaacgcc
     2641 accattagca ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca
     2701 ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc cagcataccg
     2761 atcagtttcc agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc
     2821 agcgaacctt ccagcgccac gccattgcgc tttagcccca ccacattcag tccgtagcga
     2881 gtacgaaaac caatttcgcg caccgattta ccaatcagtt ctgactcagg aattaatgaa
     2941 atctctgcca tgcccacatc aagggcctgg tcagaaaaat actcgccgcg cagtaccatc
     3001 ggctccagca attgctcact acaaaattgc cggagatcga catcagccgc agacatatca
     3061 ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga
     3121 cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg
     3181 agatcatcca gccgttgacc aatcattggc gatccggggc gaatagccag acgtcgcgca
     3241 cgcccggtca gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg
     3301 cgctgcgggg tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata
     3361 cccagcacca gcacgaccag gccaataggt gttacgctaa agaaactgaa gccgtgatag
     3421 ccttcacgca gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc
     3481 atgccgctta tcagcccggc aaaactcaac ggcatcatca gacgtgacgg cgaagtttgc
     3541 atacgcatgg caacgcttaa caccacggga ataaagatag cgacaacgcc ggttgaactc
     3601 ataaacgcgc caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg
     3661 ttgcccgcaa ctttgaccag ccatgttccc attacggtgg caacaccggt acggaccaaa
     3721 ccatcgccaa taataaacaa ggcggcaatc aggacaacgt taggatcaga aaagccggaa
     3781 aatacttcgg ggactgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg
     3841 accgcatcca tacgcactct gcccgtcgca aacaagacaa tggcaacggc cagtaatgaa
     3901 agaacccaaa tcaattcacc gttcacaact tatccttgtt aattgaggga gatgtcttga
     3961 ttctgccata aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa
     4021 taataacatc gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca
     4081 aatcgacctc attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatgacaac
     4141 ccgccgccag gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg
     4201 gcgcaagccc cagcagctgc gcgcctaaca gatacgcatc aggttctggt tttccgcgct
     4261 tcactcgctc agcggttaca aacacctctg gtgcgggaag cccagctatt ttatggcgcg
     4321 ctcgcgctac cggcatggag ccagaagtca caatggccca cggaatacct gctttattca
     4381 aatgactgag taaggcgatt gcccccggaa gcgcggtaat accttcggtt tccgtggcct
     4441 cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg cccgccataa
     4501 aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg
     4561 ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc agggaatcca
     4621 ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc tcctcaggca
     4681 ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac
     4741 acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa accgtgagtt
     4801 ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac ataaccgagg
     4861 taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat
     4921 tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc cataatgtcg
     4981 atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc
     5041 tgtaatccgt aaccacgctc aataattgtt tgcaggcgac ggtaacgttc agcatttgcc
     5101 ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg cgcgttggtc
     5161 atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag gctgagtatg
     5221 ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag
     5281 ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac
     5341 gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa accagttaag
     5401 gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccataatc cctgcatggg
     5461 taaacttaag gcgaacagcg cagtggcaac tgccggccca agctgaccgc ccagggcaat
     5521 ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat
     5581 cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt
     5641 tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt
     5701 tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact
     5761 cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca
     5821 gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat
     5881 tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa
     5941 atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc
     6001 tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt
     6061 actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg
     6121 tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg
     6181 gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc
     6241 gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac
     6301 tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga
     6361 tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc
     6421 tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt
     6481 tgctgtattt gacaccgcgt tccatcagac tatgccggaa gagtcttacc tctacgccct
     6541 gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca
     6601 cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat
     6661 catcacctgc cacttgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt
     6721 tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc gttctggtga
     6781 tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat
     6841 caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg
     6901 ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta
     6961 ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga
     7021 cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg aactgtctct
     7081 gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacttgg ctgcacgttt
     7141 cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta ttccaaccaa
     7201 cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag
     7261 ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc
     7321 gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc
     7381 cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc
     7441 gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac
     7501 tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc
     7561 agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa
     7621 gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag
     7681 tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag
     7741 ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc
     7801 ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat
     7861 gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa
     7921 gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct
     7981 gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat
     8041 gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc
     8101 gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc
     8161 gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc
     8221 ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa
     8281 cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacctct
     8341 ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg tatcgagaaa
     8401 gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct gactgccact
     8461 tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac tgaacttgcg
     8521 cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca
     8581 gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag
     8641 atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga aatcgttgat
     8701 ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc
     8761 atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg
     8821 ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc
     8881 atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg
     8941 ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat
     9001 ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc
     9061 ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgcaggtagc
     9121 gacgtagaaa aagttcgcga agcaactcgt ctggcacagg aaaaacgtcc tgacctgatg
     9181 atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg
     9241 ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt
     9301 aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg
     9361 cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc
     9421 tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatccg
     9481 cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt ttttattctc
     9541 ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg
     9601 ccatctcact ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc
     9661 gcaatcactg aatctaaacc aaccagaaga tgggcaatgc ccgatacgtt ggtgattatc
     9721 ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg gatgtttgac
     9781 agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca
     9841 cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg
     9901 ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg gttaacctca
     9961 ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat tggcggcgcg
    10021 tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat
    10081 acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc
    10141 gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc accgctaatg
    10201 gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc cacgcaaatc
    10261 ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc
    10321 gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactatgatt
    10381 ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc
    10441 gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt
    10501 acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg ggtgatttgg
    10561 ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt
    10621 ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg
    10681 gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc
    10741 gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag cgtgttaaat
    10801 accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt cgcggcctgg
    10861 tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg
    10921 gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt
    10981 accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc aacctcagct
    11041 tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct gaaggtgggc
    11101 gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg cgctcagttg
    11161 atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt
    11221 tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag cgctttcagc
    11281 gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact
    11341 tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct gaacaacgcg
    11401 ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca
    11461 ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg
    11521 gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg
    11581 gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga
    11641 tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac ttcgttctct
    11701 tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa
    11761 ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc
    11821 gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc
    11881 agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc ggggttgaag
    11941 tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta caccagcaca
    12001 tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg cgtcaaaaac
    12061 agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc
    12121 atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg tgccacttca
    12181 ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg
    12241 tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc aaacaactcc
    12301 agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc
    12361 actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc
    12421 agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt
    12481 cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc ggttaatcat
    12541 tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtgc
    12601 taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct cggccgttag
    12661 tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc ggatgggcat
    12721 cagatcgctg cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag
    12781 cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc
    12841 agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc gtgtctgacc
    12901 ataaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg
    12961 aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag gggagaaaaa
    13021 gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg caataaaggg
    13081 ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgtaag
    13141 tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc tgcaatgatc
    13201 gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct cgaagaagca
    13261 gagcttgatt atcgcttgat taaggtaggc ctggggaaag gcggtcagtt tcgtccggaa
    13321 tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc tcctgccgat
    13381 ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct ggctgagaaa
    13441 acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca gtggttattc
    13501 tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa tcacgcagcc
    13561 ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctttaccat
    13621 gtactgaaca agcggctgga aaactcgccc tggctgggag gcgagaacta cagcattgcg
    13681 gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga cctcgcaatg
    13741 tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac cgggcaggca
    13801 ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg
    13861 tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct gccgctatta
    13921 ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa gaaattaaca
    13981 accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa gcgcgcacta
    14041 gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt attcagcatg
    14101 tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc gatatcgcac
    14161 gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac gcgctgcgct
    14221 acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat cgggaggctg
    14281 catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga ttccgcgttt
    14341 gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag ccagttccgt
    14401 tctcggccct cgggtgacgc tatggcaagg gcttgccgat caaagcaacc tcaacggcgt
    14461 tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga ctcacgagca
    14521 aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa
    14581 tgccagcgac acgccaccgt cggtactcat ttccggctcc gcaacgggct attatggcga
    14641 cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta cccataaact
    14701 ctgcgcccgc tgggaagaaa ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct
    14761 gctgcgtacc ggtgtagtgc tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc
    14821 gtttcgtctt ggcctgggcg ggccgattgg ctctggtcgg cagtatctgg cctggattca
    14881 tattgatgat atggtcaacg gtattctctg gctgctggat aacgagctgc gcgggccatt
    14941 taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc tcggtcatgc
    15001 gctgcatcgc ccggccattt tgcgcgtccc cgcgaccgcc attcgactgt taatgggcga
    15061 atcttcagta ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg
    15121 ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg
    15181 tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc tttgcgcgat
    15241 aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta aacctctatc
    15301 cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg cgccttcttc
    15361 acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc gttccgctat
    15421 ctcaccaata aatgcacgaa aacgctgggc atcccctgtt tgtaatacgt aattaaacag
    15481 ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa tttggtcgac
    15541 taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca gcgccatttt
    15601 gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg gaaacgctga
    15661 tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc agagtgaata
    15721 aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg gaagctcttt
    15781 atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc
    15841 cattaattct tccggcttac tttggtgctc tatcactaca taaatataac ccactccctc
    15901 ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt catcaataaa
    15961 actgtttggt tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag
    16021 atgaatatca ataaaatccc gcgcggtgtc tggatggcgt aaaaaagatt taaataccac
    16081 atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct caaatttaag
    16141 atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta tctttggaca
    16201 gcgaattcag aagtattttt agcggactgc aacttttaca tttcagcgga agctgactcg
    16261 caaattcccc atgaaaatac tccggcagcg ttagccaccg gagaggaaat tatttcagcg
    16321 atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac tgctccggcg
    16381 cgccctcttc ttctattttc ccctgatgga ggaaaatgac gtgagtagaa acatggcgag
    16441 caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc agttgctgca
    16501 taatacgtaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt tcatcaaaca
    16561 gtaaaacttc cggttccatc gccagcgccc gcgcgataga aacacgctgt tgctgaccgc
    16621 cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact ttcgccagat
    16681 acttcaccgc ccgctcgcgc gcttcctgct tgctcaggcc caacacctga atcggcgctt
    16741 ccatgacgtt ttccagcacc gtcatatggc tccagagatt gaagtgctgg aataccatcg
    16801 tcaggcgtgt gcgcagtaag cgcagttgat ttttatcggc gactttgagt tgaccgtctt
    16861 tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgacccttca ctcggttttt
    16921 cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat ccgatgatgc
    16981 ttattacatc tccggcattc gcttgcagtg agaccccttt cagcacttca tgttcgccgt
    17041 agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc tcagtgcgtt
    17101 gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag gctgatcagg
    17161 acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa aggttgatac
    17221 gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc cgtaaacgcc
    17281 agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa cgcaatacgc
    17341 agcgcagaag gcaaaataat gcaacgatac attttaaaag tcgagaagcc ataggctctg
    17401 gcggcttcaa tttccccatg cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg
    17461 taagcgcagg tgttaagcgt cagcgccagc acggtacagt tcaggccact gcggaagaaa
    17521 gcgttaagga attcggttcc cttaacaatc tcaagcgtgt acatgccgga atagaacacc
    17581 agcaactgaa catacagcgg cgtaccgcga aaaatatagg taaataacca gattggaaac
    17641 tggatgtatt tattactgga gacacgacca atcgccagaa acagcgccag gactccgcct
    17701 atcactaccg acaaaataag cagccacaga gtgatcgcca caccagtaaa gcgataaccg
    17761 tcggtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg tcagccctct
    17821 tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa acagtggtga
    17881 aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag gtacttttgc
    17941 ctgccagttg ggtggctttg accacatctt ccaggccgag taacgaaacc agtgcggtag
    18001 atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg
    18061 gaaacatgat ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc gccgcctcta
    18121 tatgcccttt cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt
    18181 agataaaacc gagagtaata ataccagcga ccatcggatc gatatcaatc tgcccgacgc
    18241 ccatcgcctc cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatc agcagcatca
    18301 acaccaagtc cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa
    18361 gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca gcgagcacta
    18421 cagaactgat agccagctcc agcgtgacga gcgcaccctg taaaataaca cctgaaaacc
    18481 catacaacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg
    18541 gttacccgaa gggagcgggc tttctttcac cgatggcgat taaccgccat aaacatcaaa
    18601 atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt cggcaaaggc
    18661 tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc cggtccctac
    18721 accaaacagt ttttcatctt taacagacgg gccaccgaat ttgtaatctt taccgacggg
    18781 ttgtttgagg aaaccttcac tggcagcgac ctcatcctgg aacgcggcat caatacgtcc
    18841 ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt caatgccttt
    18901 tggtgcccag tgttcgttac cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg
    18961 cttgcctttc agcgactcga ctgtcggctg aatgtcggaa tttttcgcca ccaccagacg
    19021 agaatcggca gcgtacagtt tgtcggtgaa ggcaatttct tgctgacgtt tttccgtaat
    19081 ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga ttaacgcatc
    19141 cagcggattt tcgacaaacg tacattgcgt attgatgcgc ttgcacaact ccttcgccag
    19201 ctcgatatcg aaaccaacca gttcgccttg tgaatttttt gattcaaatg gcgcataggt
    19261 cgggtcggta ccgatgcgga tgttttgcgg aattgcagca agtgccgcag ttgcgctgga
    19321 gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct caactgacag
    19381 tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa attgaccatc
    19441 aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc ttaagtatgc
    19501 atttcaatgc accacgatag tgcaacaaac ctggcgtgca ccatgatgat cattccatca
    19561 ggtacagctt cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa tactttttcg
    19621 ccatcttgtc gtaggtgccg tcctgacgca gctcgccaag cgccttattg aaggcagccg
    19681 tcagttcagc atcatcttta cgtagcccta caccggtgcc atcaccgaag tattttttgt
    19741 cttttactga tgagccagca aaggcgaaat ctttaccagc aggttgcttg aggaatcctt
    19801 cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc agatcggaat
    19861 agaccaaatc ctggttggca taggccacca catccacgcc tttgctacgc caggtctcgt
    19921 tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct ttcagtgaat
    19981 ccagcgttgg ctgaatcggt gaacctttgg cagcaatcaa acgagaatct gcggcgtaca
    20041 gcttgtcgga gaaggcaatc tcctgctgac gtttatcggt aatggaaagc gacgaaataa
    20101 tggcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc
    20161 aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata tcaaagccaa
    20221 caaaatcacc tttagcatct ttcgacgaga acggcgcata ggtggtatcg gttccgatac
    20281 gtaccgtctc cggtagcgcc gcatagctgg aagccgctgc ggagagaccg actaacaaag
    20341 acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct tttttatgtt
    20401 gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat cttgacgaca
    20461 acagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtcc tgaagttgaa
    20521 gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa caaaatgtcg
    20581 aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag ttatgcgccc
    20641 tgccagcggg caaagagatc ttcaggaagg gttattgcaa actggtcaag aacacgatta
    20701 accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc cggaacggga
    20761 ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc
    20821 aatggtgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac cacatctgcc
    20881 gcacgggtca gtaagccatc agtatagcta tggacaatgc cggaaagggt tttgattgaa
    20941 cagggtaaaa tcaccatccc cagcgtctgg aaagaaccgg aagagatgct ggcggaaata
    21001 tcgcgcgcat cgtgcgtgac atcagctaat gcctgcactt cgcgcaggga aaaatccgtt
    21061 tcgagggata aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg
    21121 acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc accgctgatg
    21181 cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag actttgcagg
    21241 atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctgagcgaa ggcatcatcc
    21301 ttcgttatgc atttcgagat tttccacttc gttctggcgc tgcaccgctt tggcgtcgtc
    21361 attacgtaaa gtatcgagga aatcgaggta gccctgatca acgtctttgg tgacgtatac
    21421 gccgttgaat accgagcatt caaactgctg aatgtccggg ttctcagcgc gaacggcttc
    21481 gattagatcg ttcagatcct ggaaaatcaa accgtcagaa ccaatgatct ggcgaatttc
    21541 atccacttca cgaccgtgag cgatcagctc cgtggcgctt ggcatatcaa tcccgtaaac
    21601 gttcgggaag cgaatttccg gtgccgccga ggcgaggtac actttcttcg cgccagcttc
    21661 gcgtgccatc tcgataattt gctcagaagt ggtgccacgg acgatggagt catcaaccag
    21721 cagaacgttt ttatcgcgga actcagcgcg gttggcgttc agtttacggc gtacggactt
    21781 acgacgcagc tgctggcccg gcatgatgaa ggtacgacca acgtagcggt ttttcacaaa
    21841 gccctgacgg tacggtttac ccagaatacg ggcgatttcc agcgcgatat cgcaggaagt
    21901 ttccgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg caattttctc
    21961 gcccagcttc gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga
    22021 gtccgggcga gcaaagtata catactcaaa caggcacgga ttgctgaccg gattgtcagc
    22081 acattgacgg gtaaacaact gcccttcttc agtaatgtaa atcgcttcgc caggcgcgac
    22141 gtcacgcagg aaatcaaagc ccagtgtatc gagcgcaacg ctttcggaag cgaccatata
    22201 ttctgtacgg ttctcgtcaa tatcacgttt tcccagaacc agcggacgaa taccgtttgg
    22261 atcgcggaaa gcaaccatac cgtggccgat aatcatcgcc acacaggcat atgcgccgcg
    22321 gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca gcgggtagtg
    22381 gcggaagttg tccagctcgc tagcgaagat attaagcaga atttccgagt cggaagtggt
    22441 gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag cgttggtcag
    22501 attgccgttg tgggcaagcg taatgccata cggagagtta acgtaaaacg gctgcgcttc
    22561 agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca tattgccctg
    22621 caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg ctttacgcaa
    22681 acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac cgcgatgctg
    22741 aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg cgataccgac
    22801 aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt acgctctggg
    22861 caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa aactgaattg
    22921 cgggatcagc tgtgatttgc tccagtcttc gcttttcgac accccggtaa aggagtcgag
    22981 aaagaagaga atggcggcaa caatcaacac accgcgcaac gcaccgaaac agacgcccag
    23041 cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca cgaagttcac
    23101 gatagcacca acgatcaggg tagcgataaa cagtaccgcg atggcaatcc catttcgaac
    23161 cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat gactggcaac
    23221 aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc cgcggatcag
    23281 gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc agaccatatg
    23341 tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa
    23401 cgtttgcgta gggatttcct tcccacgcat caataaaaat ggcgctgaaa aaatattcaa
    23461 cgccatcgac tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac
    23521 gccttatccg acctacggtt ctaacactgc ccaggcctga taagacgcgc cagcgtcgca
    23581 tcaggcaaga ctgtattaat tcggcgtata gcccattacc acgccactta agccagaaag
    23641 ttgcttcaac tcacccagcg aacctttcag cttatctttc gaggcatccg gcccaaccag
    23701 aatacgggta attttaccct gcactggcgt ggatggcgac gtataaaccc gataaccggc
    23761 accgcgcagc ttaccgacaa tctcattcac tttatcggca tttttcagcg cacccagttg
    23821 cacaacatag gctttacccg ttggcgctgc tttttcttcc acgaccggct tcggttctgg
    23881 cttcggcgca ggtggtgctt caaccttcgg tttaggcggc tccaccggtt tcggcttcgg
    23941 tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa tagtggccgg
    24001 atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc cttccggcgg
    24061 ctgcgtcggc aacgcctggg tggcggctgg catcatatca ggctcatcgc gatcgcccgc
    24121 ttttggaacc agcgggatag ccgcaaactc atcctgataa tgttttttct gcccgtccag
    24181 cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca ctaaccgatt
    24241 ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca tgtgcgaccg
    24301 tgtggaaaga accacacacc agcacggtgt cttccgcttt agcgtccgcc attgcggcat
    24361 cccatgcctg cgcaacgcta tcaaatgatt tgccgttacc caaatgctca agcagttgtt
    24421 ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca accacgcttt
    24481 tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata accgccagca
    24541 cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc gcatgtggat
    24601 tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa cgtcccggca
    24661 aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacttccagc ccgctggcac
    24721 gcagtgccgc cagcgctgtt gcggcattcg gttgcgggac aagcggcaac ggcagatttt
    24781 ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa tagttccact
    24841 caacgccccg acgttgtaac agtgcacctt tttcctgcgc cacatcagca atggtagaag
    24901 gcatttccgg ctcaccgaca attgccggtt ttgcgctgcg aaagatgcct gctttctcgc
    24961 gaccaatact ttcgcgatct ggtcccagcc agtcggtatg atccagcgca atgctggtta
    25021 ctacagccac atcggcatcg acaatattgg ttgcgtccag acgaccgccc agccctactt
    25081 ccagaatcac tacgtcaagt tgtgcctgtt tgaacagcca caacgccgac agcgtaccgt
    25141 actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca aaagaggcgg
    25201 tgtgggccga ttccggcaat tcctgcccct gcacacgtac gcgctcggta taacgcacca
    25261 gatgcggtga actgtagacg cccactttgt accctgccgc catcagaatc gactccagcg
    25321 tacggcaggt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt
    25381 tcaggacgcc aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga tcgatagttt
    25441 tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg gcttgaggag
    25501 tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa aagggcagag
    25561 ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatccggt accgggggta
    25621 ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc aacttcgcca
    25681 gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg atggcgcctt
    25741 tctcgatcag gaattcactg cgctggaatc ctggcggcag tttttcgcga acggtctgtt
    25801 cgataacacg cggaccggca aagccaatta acgctttcgg ttcagcgatg ttgagatcgc
    25861 ccagcatggc gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt
    25921 acggcaagcc gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc atctgcatca
    25981 gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag atcagtgggc
    26041 agttatcttc cagcgcctgc tcaacggcac gcacgaaacg tgcacccaca acagacccca
    26101 ttgaaccgcc cataaaggcg aactcgaatg ccgcagcgac aaccggcatt ccatacagag
    26161 tgcctttcat caccaccaac gcatcttttt cgccagtttc tttctgcgca gatgccagac
    26221 ggtctttata cttcttggag tcacgaaact tcagcacatc tttcggctca agctcgctac
    26281 ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc gctgtcatac
    26341 gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca gcgcggtata
    26401 aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc gccttgcggg
    26461 tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt tctgtctgaa
    26521 cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc ccccaacaga
    26581 ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa agtggtcgaa
    26641 ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac ggattatttc
    26701 gatgacaccc ggcaatatag aaaccacaat aatcccgacg atcagcagct taaggttatc
    26761 ctgaaccatc gggattgtac cgaagaaata gcctgcgtag gtaaaaagca gtacccacaa
    26821 cagtgcgccg atcacgttat aagcggcgaa atgacggtac gacatgtggc ccattcccgc
    26881 aacaaacggg gcgaacgttc tgacgatcgg cacaaaacgg gcgagaataa tcgttttgcc
    26941 gccatgtttc tcataaaact gatgggtttt gtcgagataa ctgcgacgga aaattttcga
    27001 attgggatta ctgaacaact tttcaccgaa caaccgcccg atagtgtaat tgaccgcgtc
    27061 acccacaatc gcggcgatca gcatcagcac caccatcata tggacgttga gatcgttggt
    27121 ttccagtgat gccaacgctc cggcaacaaa cagcaatgaa tcacccggta aaaacggcgt
    27181 taccaccaga ccggtttcac agaacaaaat taagaacaaa atggcataaa cccagacgcc
    27241 gtactccgcg accagctctg ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa
    27301 ataaatcagg tccatatttg cctatgcctt gtactcgtca tctttcaggc tgtaactacg
    27361 ttggctacag cctgaaatgt tccgagcatt attctcgtta gtccgccaga aatagcgggc
    27421 ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc agatacagcc
    27481 cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc aactctgcta
    27541 tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca atattcctga
    27601 ccatatgatg tacaaaagca ttcgctttaa tatctaccac cacataagga ccgtgacgcg
    27661 tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc accgcacgga
    27721 acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt
    27781 ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc agccgatgat
    27841 tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga aaatcatcag
    27901 gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt acgcccaagg
    27961 tccacgccgc gtctttgcgc tgcgcggttg tttcgaaatg caccacttgc ccggtaccgt
    28021 gtacccctgc gtcagtacgc ccggcgcaga agacggtgat gggttcgttc gccacctggg
    28081 agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt tgccagccgt
    28141 aatacttact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc agttgttgct
    28201 ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta ctgccatcag
    28261 cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tggacttgct ccggcatacc
    28321 gtagtcatta cgcacgcagc caacagaaag atgcggcgta cccgaagcat cccctacctg
    28381 agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa acgcatcacg
    28441 cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg catgaccgta
    28501 gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct gcaggatttt
    28561 gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat ccggcaacaa
    28621 cggcagcata ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc
    28681 gttgagcaat ttcgcactct gccccgctaa cgcatcgacc gcttttttgc cctgggcgga
    28741 ggctgaaatc aggctggtaa cactgatacg cgacaaaccg ccctgatcga ttaacggttt
    28801 cagtgccgcc agcagctggc tggtcagact gtctggtacg gcgatgacat tccggttgcg
    28861 gtaatcggtc agtacaaacg ggtttacttc cggcaccacc agcggtacgt cgggttcgag
    28921 agcaaacagt ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt cttcaaccca
    28981 ggctgcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa
    29041 ttcagcggca tcctgcacgg tgattgtctt accaccaaag cgcagttgtt cgcctgcgct
    29101 ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag ccagcgtttc
    29161 aagcagggct tcgcccacag cgccagttgc gcccaggacg gcaatgttcc agccttcaga
    29221 catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca gagagcatga
    29281 agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca cagcaatgat
    29341 gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg ctcaagatag
    29401 tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag cggtgcgtca
    29461 tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg atcgagcggg
    29521 ccatgcagcg taatgcgacc aaactctggc gcgggcagta atgtatccag cgcaacgtgc
    29581 tgttcatgcc caataaactt gctataggct tcaaacactt gcgtagtacc gcgtgcttta
    29641 ccttccaggg tataacctgc gatatgcggc gtgccgatat ccactttttt cagcagctcg
    29701 acgttgagtt ccggttcgcc ttcccagaca tccagcacta cgcttaactt ctggccttca
    29761 ttcaggcagg tcagcaacgc agtattatcg acgactgcgc cacggcaggc gttaatcaga
    29821 atcgctcccg gcttcaggct acggatcagt ttttcatccg ccagatgcag cgttttgtac
    29881 ggcccgtctt tgaagagtgg cgtatggaaa gtcagaatat ccgcatgctg aactaattca
    29941 tccagcgagc ggaaatcacc ctcatcccca cggtcagcgc gaggcggatc gcaaagcaat
    30001 gttttaatcc ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc
    30061 acgatcccca ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag catcagtagt
    30121 gaggagaaaa catattccac caccgcagtc gcattacagc caggtgccgc ggaaaaacca
    30181 attcccgcct gctttaacca tgcttcatcg acatggtcgg tccccgctgt ggcagtacca
    30241 acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac cgaacgcacc
    30301 atcagcgcat ccgcgtctgc cagttgagcg acggggattg gacgcccggg aaccgcagtc
    30361 acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc aacaaggatt
    30421 ttcacgtttg tgttacctgt atgagacgag agttaaccgg acaagtgtgc cataatctcg
    30481 cggccaggca tacttgcgaa gatttcaggt ataaggatac gtaatgatac aacctatttc
    30541 cggccctcct cctgggcaac caccaggtca gggagataac ctgccgtctg gcgcgggcaa
    30601 tcagccttta tccagtcagc aacgtacttc gctggaaagc ttaatgacga aagtgacctc
    30661 actgacgcaa caacaaagag cagaactgtg ggcgggtatc aggcacgata ttggtctgtc
    30721 gggagattca ccgctgcttt cgcgtcactt ccctgccgct gagcataatc tggcgcaacg
    30781 tctgctggcc gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat taggggagta
    30841 tttacgtctg gggaataatc gtcaggcggt cacggattat atccgtcata actttggtca
    30901 gacgccgctg aatcagctct caccggagca attaaaaacc attctcaccc tgttgcagga
    30961 agggaagatg gttattccgc aaccacagca gcgcgaggcg accgaccgtc ctttattacc
    31021 ggcggagcac aatgcgctaa aacagctggt gaccaaactt gcggcggcaa cgggggaacc
    31081 cagcaaacag atctggcaat cgatgctgga actttccggg gtgaaagatg gcgagttaat
    31141 tccagcgaaa ctgtttaacc atctggtgac ctggctgcag gcgcgccaga cgctaagcca
    31201 gcaaaatacg ccgacgctgg aatcactaca gatggcgcta aaacaacctt tagatgccag
    31261 tgaactggcg gcgttatcgg catatatcca gcaaaaatat ggcctttctg cgcaatcatc
    31321 gctttcttct gcccaggccg aggatattct taatcagctt tatcaacggc gggttaaagg
    31381 gattgatccg cgtgatatgc aaccgctgct taatcctttt ccaccgatga tggacacgtt
    31441 gcaaaatatg gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct
    31501 ggtctggctg gttcgttaac cccgacgaaa tgacagtatc gtgacaataa tacccagcac
    31561 cgcagagatc gccccggcaa gaaataccga agagtaacca aacgtggtcg ccagcattcc
    31621 cgccagcggc ccggagacgc cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc
    31681 ggtgccgcga acttgtgagg ggacgcgttt aaccacctcc acgcccagcg caggaaagat
    31741 aagcgaacat ccggctccgg ttaacgccgc gcccgctaat gcgacccacg cccctggggc
    31801 ttgccagagc agcaacaagc ccaccgtttc tacaagcaga gagacaatcg ccactttcac
    31861 gccgccaaaa cggtccggca tccagccaaa catgacgcgc atcacgacaa atgcgccgcc
    31921 aaacgcggta agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac
    31981 gaaagtcccg ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc ctggtttcca
    32041 gataagcccg acaacgctcc acagcgatgg acgttctccc gccagggccg gtactttgcg
    32101 cactgtgccg ttacaggccc acgccagtaa gggtaatacc attgtggtga tcgccagtgc
    32161 ggcaaaaccg taatggctat gaatcaacag gccaagcgga gcaccaacag cgagggcacc
    32221 gtaaatcgcc attccattcc atgacatcac tttgccagag tgttttggcc ctacgatgcc
    32281 taacccccag gtcagagcgc ctgtcagtaa ctggctttca ccaaagccaa gaatcaaacg
    32341 cccgacgacc aacagggcaa atttgaacgg tgcggagacg ggcaaaatcg ccgccagcag
    32401 caacgcgccg ccagccagac cacaagctaa catcccctga agagccgaac gttttgcgcc
    32461 atattgatcg gccagtcgcc cggcgtaacc acgcgtcagc accgtagcca gaaactgaat
    32521 cccgacggca atgccgacca tggtattgcc atagcccaga tcatgatgaa caaacagcgg
    32581 gataaccggc aacggcagcc ctacggtcat gtaggtgaga aaaaccgcaa aagcgatgcg
    32641 gaagagcgaa aaattggcag aagatcgtgt ttcggtttgg cttacagcag tcatgcatta
    32701 ctccagaatg cagcgcaagg cgaggagtat ccccgtctca tctctctggt ttcagggtta
    32761 cggtgcgttg gcaggattta acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa
    32821 gtttgcctgg aacaggcggc gattgtcaat gatgtgaaaa agggaaccat caggttccct
    32881 tttgcgttag tgccggaggc cgcattagcg cgtaacgtcg gatgcgacgc tggcgcgtaa
    32941 cgtcggatgc gacgctggcg cgtcttatcc gacctactcc gactactccg acctactgcg
    33001 aattaatctt tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa gccgaagctg
    33061 ttagacataa cggtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt cagacccgca
    33121 gcctgctcgt ccagctcttc aatgttgatg ctcggggcga taaagccgtg ttccagcatc
    33181 agcagagagt agatagcttc ctgcacaccc gcagcgccca gtgagtgacc ggtcatggct
    33241 ttggttgcag aaatcgccgg gctcttatcg ccgaacactt cacggatagc tgccagttct
    33301 ttcacgtcgc caaccggagt cgaagtaccg tgagagttca ggtaatcgat tggggtatca
    33361 acgccatgca tcgccatctg catacaacgt actgcgcctt cgccagacgg agcaaccatg
    33421 tctgcaccat cagaggttgc gccgtagcca acgatttcag catagatgtg agcaccacgc
    33481 gccagcgcgt gttccagctc ttcaaccact accataccgc cgccgccagc gataacgaaa
    33541 ccgtcacggt gagcgtcgta agtacgggag gctttttccg gggtgtcgtt gtatttagta
    33601 gacagcgcac ccatcgcgtc gaattcgcag gccatttccc agcacagctc ttcgccgccg
    33661 ccagcaaaca cgatgtcctg tttgcccagt tggatctgct ctactgcgtt accgatacag
    33721 tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa cgccatgaat tttaaacggc
    33781 gtggcgaggc aggcagaaac gccggatgcc atcgctttgg tgaccacata cgggccaacc
    33841 gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga atacctggaa acgcggggaa
    33901 ccgccgccag aacctgcaat caggccaacg cgcgggttat tctggtaagc ttccggagaa
    33961 aggcccgcat ctgcgattgc ctgctccata gaaaggaatg cataaatgga tgcgtcgctc
    34021 ataaagcgca caactttgcg gtcaatgagg ccagtggtat ccagttttac gttgccccag
    34081 acgtggctac gcatgccgga atccttcagc tcctgagaga aagtgatccc tgaacgtcct
    34141 tcacgcagag atgccaggac ttcctgctgg ttattaccga tgctggaaac aatgcccagg
    34201 ccagtaatca ctgcacgttt cattcaatac ctctgtaagt cgcacataga gtaagtttcg
    34261 aatgcacaat agcgtacact tgtacgccga acaagtccga tcagccattt aatagagaaa
    34321 tttgcgcagc cttacacaca tcgctaagat cgagccaccg cctgtaagac gagtaactta
    34381 cgtgaaacac tactccatac aacctgccaa cctcgaattt aatgctgagg gtacacctgt
    34441 ttcccgagat tttgacgatg tctatttttc caacgataac gggctggaag agacgcgtta
    34501 tgtttttctg ggaggtaacc aattagaggt gcgctttcct aagcatccac atcctctgtt
    34561 tgtggtagca gagagcggct tcggcaccgg attaaacttc ctaacgctat ggcaggcatt
    34621 tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt tcattagttt
    34681 tgagaaattt cccctcaccc gtgcggattt agccttagcg catcaacact ggccggaact
    34741 ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg cccttgcccg gttgccatcg
    34801 tttattgctc gatgaaggcc gcgtgacgct ggatttatgg tttggcgata ttaacgaact
    34861 gaccagccaa ctggacgatt cgctaaatca aaaagtagat gcctggtttc tggacggctt
    34921 tgcgccagcg aaaaacccgg atatgtggac gcaaaatctg tttaacgcca tggcaaggtt
    34981 ggcgcgtccg ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc gccgcggttt
    35041 gcaggacgcc ggattcacga tgcaaaaacg taagggcttt gggcgcaaac gggaaatgct
    35101 ttgcggggtg atggaacaga cattaccgct cccctgctcc gcgccgtggt ttaaccacac
    35161 gggcagcagc aaacgggaag cggcgattat cggcggtggt attgccagcg cgttgttgtc
    35221 gctggcgcta ttacggcgcg gctggcaggt aacgctttat tgcgcggatg aggcccccgc
    35281 actgggtgct tccggcaatc gccagggggc gctgtatccg ttattaagca aacacgatga
    35341 ggcgctaaac cgctttttct ctaatgcgtt tacttttgct cgtcggtttt acgaccaatt
    35401 acccgttaaa tttgatcatg actggtgcgg cgtcacgcag ttaggctggg atgagaaaag
    35461 ccagcataaa atcgcacaga tgttgtcaat ggatttaccc gcagaactgg ctgtagccgt
    35521 tgaggcaaat gcggttgaac aaattacggg cgttgcgaca aattgcagcg gcattactta
    35581 tccgcaaggt ggttggctgt gcccagcaga actgacccgt aatgtgctgg aactggcgca
    35641 acagcagggt ttgcagattt attatcaata tcagttacag aatttatccc gtaaggatga
    35701 ctgttggttg ttgaattttg caggagatca gcaagcaaca cacagcgtag tggtactggc
    35761 gaacgggcat caaatcagcc gattcagcca aacgtcgact ctcccggtgt attcggttgc
    35821 cgggcaggtc agccatattc cgacaacgcc ggaattggca gagctgaagc aggtgctgtg
    35881 ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa catcattgta ttggtgccag
    35941 ttatcatcgc ggcagcgaag atacggcgta cagtgaggac gatcagcagc agaatcgcca
    36001 gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa gaggttgatg tcagtgataa
    36061 agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg taggcaatgt
    36121 tcccgattat gaggcaacac tcgtggaata tgcgtcgttg gcggagcaga aagatgaggc
    36181 ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg gctttaggtt ctcgcggttt
    36241 gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg aaccgattcc
    36301 gatggatgcc agtatgctgg cggcgttaaa cccgaatcgg ttatgggtgc ggaaattgtt
    36361 gaagggtaaa gcggttaagg cggggtaatc tgctctcgca gtgtttgccg gatgcggcgt
    36421 gaacgcctta tccgacctac gtgtgacgtg taggcctgat aagacgcgat aagcgtcgca
    36481 tcaggcatgg tgctccagac gccgcaactt actgctgtga cgcctgctga aacaggtttt
    36541 cccacatatc ggtcaccagc gcctggtcac gcggcgacaa ttcaccggca ccaatggctt
    36601 tttccagact ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca tcaccacttt
    36661 ccagttctgc gatggctaat gtcaggtggc cacgcaaata cccactggca aacaactcat
    36721 catcactggc atggtcaacc ataccgtcga ttaatgccag aatgcgtgat tcaaactccg
    36781 cgatcatctt ctttcctcat tgtaaaacgt ggcaccggct tcagttgagc gcttccggcc
    36841 acgggaactg ttccgccgta agttccggcg tgtgataata attttgtagt gctttaatga
    36901 agcgtgccgg acgctcggga atgccatttg tcagataatc catcacctgc gcatgtaccc
    36961 gccgttgaaa caccacacga tccggttcga agtcgccttc cagattgtcg caactgacgt
    37021 taaacggata tcccgccgcc acacagaaca accagtcgag tgcctgcggc ttcacttcaa
    37081 catcttcaaa ctggctttgc gtttgggcat cacgcccatc cgggcaatac cagtagccga
    37141 aatcaaccag ctcacggcgc gcttttccgg caatacacca gtgcgaaatc tcatgaattg
    37201 cgctggcata aaagccgtgg gcaaagacaa ttcggttata cggtacttcc gcatcagcag
    37261 gaagatagat cggttcgtcg tcgcctttaa tcagacgggt attaaattca tcggcaaagc
    37321 agctattaaa aatttcaatc aactgctcat agtggtgtgt actgttcatt agttcatccc
    37381 caaccagtgg aggatctcct gtccgtggct atcgtaaagt aatttggcac tcatcaccgc
    37441 cgagacgata acgatcatcg ggcggatcag tttttgtcct ttgctcagca ccagtcttga
    37501 acccatgcgc gcgccgagga actgccccac cagcatgaca aagcccgtcg cccaaatcac
    37561 tttgccgccg agaataaaca gcagcagacc gccaatgttt gacgttgcgt tgagtaattt
    37621 ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc gtaacgaagg ccagcgcgta
    37681 aaacgatccg gcagccgggc caaagaatcc gtcataaaaa ccgacgcagc caccggcaat
    37741 taacgcgaag ggtaaaccgt acatccggcg ctggcgatct tcttcaccca gctttggcat
    37801 cagcaagaaa tagaggccga tacaaatcac cagaatgggc aaaatctgcc gtaagacatc
    37861 agcctgaacg tactgtacca acagtgcgcc gctcattgag ccgacaaagg tcatggcaat
    37921 attgagtttc tgatcgctta aactaaccac tttgcggcga ataaagtaga tagtggcgga
    37981 aatagagccg ccgcaggctt gcagtttatt ggttgccaac gcgttagcgg gagacattcc
    38041 cgctgccatc aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg agtcgataaa
    38101 tcccgccagc atggcgacaa aaaagagaac tcccagcaac agcggggaaa ccataaacag
    38161 gctattaaac gtttccatta gatcacgtgc tcatccagta gcgcctggca ggaaggcggc
    38221 aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg gttttggagg tgcaaaccag
    38281 ctttgcagtt ctgccccgca accatcgcct ggtggcggta aaggttgatc ttcacactcc
    38341 agactatcgg caggacaacg taatcgtaca tgcatatgcg cgcgatgctg gaaccagggt
    38401 cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa ggcaaagttg ttgtttaatc
    38461 gccggattaa caaaaatgcg tgtgacgtct ttgtcctggg cggcgagttt gatcaagctg
    38521 aaaatttctg gcttccacag cgtcggaaca acgtgtttac cgtcgcggga aactaagtcc
    38581 agtgcttgcg ggcgcaaaag ttgcgcggag gtccagcgtg ttttcggcag ttgcagaaaa
    38641 atatcgacat ccagtccggt ctggtggctg gcatgaccgc cgttgaaacg cccaccagcg
    38701 ggcatcccca tatcgccaat cagcaccgtg cccatgccca gattgctcac ctggctactc
    38761 agacgctgga taaacatcac caggtccggg tgaccgaaat agcgacgctg atcggtacgc
    38821 atgacctgat aatgttcgga ctgtatcggc agcgtgtcag cgccgacgat acagccatta
    38881 gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt gggttatttt ttgccacggc
    38941 gtcgctgcca ggctagcgct actggcaagc agagccagca gcgcaatcgc ggttttgttc
    39001 attttttacc agcgtggaat atcagtcttc acatcggcat tttgcgcccg ctgccgtagc
    39061 aggtgatcca ttaaaacgat cgccagcatc gcttcagcga tcggcaccgc gcggatcccg
    39121 acacaaggat cgtgacggcc tttggtgatc atctcaactt cttcgccaaa gcggttaatg
    39181 gtacgccccg gcacggtgat actggaggtc ggtttcagcg ccatatgggc aatgatttgc
    39241 tgcccgctgc tgataccgcc aagaatgccg cccgcatggt tgctctggaa accgtcttta
    39301 gtgatttcat cgcggttctg gctaccgcgc agcgccacca cgtcaaaacc gtcgccaatt
    39361 tccacgcctt ttaccgcgtt gatgctcatc agcgcatggg cgatgtcggc atccaggcgg
    39421 tcaaagaccg gctcgccaag tccggcgggg acgccactgg caacaacggt gactttcgcg
    39481 ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt catctaacgc gtcgattttg
    39541 tccgggtccg ggcagaagaa cggattttgt tcaaccagcg accagtcttt gatttccagc
    39601 ggaatgtcgc ccatctgggt caggcagccg cggatttcaa taccaaattt ctcggcgaga
    39661 tattttttgg caatcgcccc tgccgccacg cgcatggcgg tttcgcgggc ggaagaacgt
    39721 ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc agcatggcct
    39781 ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac gctggtcggt attttcgatc
    39841 aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa caccggagag tattttgacc
    39901 tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc cagggcgacg acgatcaagg
    39961 tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc gacgatgcag
    40021 ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc gaaagagttg tccaattgtg
    40081 tttccagcca tcacggctcc gttattgttg tgtttgcgtg tttacttaat ctttataaat
    40141 cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg agcataaaca caccatcgcc
    40201 gccgttatca aactccagcc aggtgaacgg aacatccgga tattgttcca taagatgtac
    40261 catgctgttg ccgacttcac aaatcagcac accatcatca gcaaggtaat ctgccgcgtt
    40321 accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc ccagttccgg
    40381 ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc gcgtcgacat acggcgggtt
    40441 agtgacaatc agatcgtatt gcactttcgg caaatcacgg aacagatcgg aacgaatcgg
    40501 aataacgttg tggatcagac cgtgttcttc gatgttctgt tcggcaaccg ccagcgcgtc
    40561 gggagagata tctaccgcgt cgacttccgc ttccgggaag gcataagcac aggcaatggc
    40621 gatgcagccg ctaccggtac acatatctaa aatatgctgc ggttgcttgc tgatgagtcc
    40681 ggcaaattta ttgttgagca gttcaccaat cggcgagcgc ggcaccagca cgcgttcatc
    40741 gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc aggtaagcca ccggaatgcg
    40801 ctcgttgacg cggcggatca cgcgttcaac aatacggtgt ttttcgctgg aggtcagacg
    40861 cgcggtgcgc atatcttccg ggatatccag cggcaggtag agcgatggta acaccagctg
    40921 tacggcttca tcccacgggt tatcggtgcc gtgaccgtac cagatatttg ccgcgctgaa
    40981 gcggcttacc gaccagcgca acatgtcctg aatggtttgc agctcattta ctgcttcatc
    41041 aacgaaaatt ttatccacgt attcctccag ggcatgatcg caataatttc ggcggctagt
    41101 ttgccatgaa gatgacgata aatcagcatt cacgcgcggt gagtgaggaa aaatacgttt
    41161 aaaacgatcg attgtgctac gagtcgggta aactgtagga aaattagaaa tagagacaga
    41221 taaatgaaaa agaaaacaac acttagcgag gaggaccagg ctctgtttcg ccagttgatg
    41281 gcggggactc gcaagattaa gcaggacacg attgtccacc gaccgcagcg taaaaaaatc
    41341 agcgaagtgc cggtgaaacg cttgatccag gagcaggctg atgccagcta ttatttctcc
    41401 gatgagtttc agccgttatt aaataccgaa ggtccggtga aatatgttcg cccggatgtc
    41461 agccattttg aggcgaagaa actgcgccgc ggcgattatt caccggaatt gtttttggat
    41521 ttacacggtc tgacgcagct gcaggccaag caggaactgg gcgcgttgat tgccgcctgt
    41581 cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc acgggaagca tattttgaag
    41641 caacaaacac cgctgtggct ggcacaacat ccgcatgtga tggcatttca ccaggcaccg
    41701 aaagagtacg gcggtgatgc ggctttgttg gtgttgattg aagtggaaga gtggttgccg
    41761 ccggagttgc cctgaatgct ggcagcaaat gccggagcag cgtaaacgcc ttatccggcc
    41821 tacaaatatg tgataattca ataaattgca attcacttcg taggcctgat aagacgcatc
    41881 agagcaatac tacgaactac agttcattca atacatactg aaaacgacac gtcgaatcaa
    41941 tcggttgccc actactggta ttcacattca ccacaatatc gtgtagtact ttatccgtta
    42001 ccggatgacg agttggcgta accgttaccg ttgatttatc acaaccgtaa gaggtaaagg
    42061 ttaacaattc tgataccgcg cggcagtatg ctcctctgcc atcattatca gaaaaattac
    42121 tgccatcaca gagtctgccg ccttccatca agtaggtcga aagcgtggtg gtcactgttg
    42181 accccgtgga ctgaattcgt aacgccttat tatggctgac ataattttca ctccaggaga
    42241 tatcgtatga agcgaccggt atcgttgggc atattgtgtt atgcactgta ggggccgtat
    42301 agcttccaaa tccgggtaaa tctgtatagg aataaaagga tgcgatccaa gggcaaccat
    42361 aatcatcaga tgcaacggta attttaaacg tcatatatgc acaacgaaaa attttcgtat
    42421 ctatgccagg gcaatttgag ttattaattg tatcaaagtc agtcagtacc aaagttggag
    42481 ctgaacccga cccagaccag gaaataaccc gcaacgtatc gcctttatac gcaaaagaat
    42541 catatttccc atatgcaacc tcctttggcg ttatcaccgt aacattgaaa acactgctcg
    42601 ctacagcacc ggttaccttg tccatggcat aataaactct cacactgtca aaactcgcac
    42661 tcataagttt atctccagca taactcttag atgcgccgac aacactcaga acgagcagca
    42721 ttaggctcgc gaaaattatg gtccattttt tcatttcaga aatactcaaa ggttgccgtc
    42781 actgtggcac taaagtctcc ggacgttaca tctcgcccgc tctttgcctg taaccaggta
    42841 ttaaaattta ggctgttatt tccctgattc agcgggagcg tgacgcctgt tgtattgttg
    42901 attggcaccg atgtgcctgc cgcagtttca ataccaatcc cagcgccgga tgctgatgac
    42961 gaggagtcaa aggccagaaa gccgggtaga gcactatctt ctgttcctgt caacgttact
    43021 ttgacattaa acaatgtaga actatgacaa tctttcaatt gaaacaccaa tggcactcgc
    43081 tctgactgcc caaggttcat taaatcgctg gcggcaatgg caggaaaatg tacttctgcc
    43141 aactggctac cattaatcac tggcgtacac gatttactga ttaaattgcc ggtaaagtgc
    43201 aaattatctc ctgctattgt ggttccactg cccagcaaca tcaggctact aataaaaaac
    43261 aacgtacgct ttttcttcat tggtaatcta cctgtaacgt ggcccaggcc tcaaagtccg
    43321 cctctggcag aacgacacca ctctttttta ctggcacagc cgttaacacc ggtaaatcgg
    43381 tttcattcac cacaagtggc gtattgatcg taaaactctg ccctgcctgt ttgagctgaa
    43441 tgcctaaacc agtgatattg ctgcttactg cagcattatc aaaatcgctt ggtgtgccac
    43501 tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc cgtatcgcag gtaatggaat
    43561 acggcacggt ttgctcataa ttgctgccat taatgtcatc aataagcaca tcctgaaaag
    43621 ttatttcaat ggcttgagcg ttgctaatcg tgcagttagg cggctgcact aaataaccgt
    43681 gaaaagagac gtttgagagt gccatcgagc aaaagcccca cagcaaaata agcgagatcc
    43741 gtttcattgg taatccacca caattgtggc actggcgtta aattcagctg ccgctaacgt
    43801 tgtaccactc tgtttcaccg gcacagcttc cagaacggga actcccgagg aaaaattaaa
    43861 tggcaaccat gagccagagg ttaagtcgag tattgtgtga tcactacttt tctgaacccg
    43921 aataccaaac ccaggaatcc ccgtgctgat aactgtttca ccgttaatca cgaccgttgt
    43981 cgcctgcatc tgtagacgca ggtcatccat tgccagattg ttacaaacaa gcgagtagtc
    44041 aatgggacgt ttataatcaa ccccattgat cttattaatg aatacattac caaactcaac
    44101 tgccgcacca tttaccgtac aaggaggagg ggcatcgaca agcacccgta aattcatact
    44161 tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca caacatatca gtaacccagt
    44221 taccgcccag acagaacaag atcctttcat actggctctc ccggcctcag cctttgcggc
    44281 tctgctcttc atcgacctgg caactgttac cgttgcaagt gaaaaagagc ggcatccgag
    44341 cgccatagtc gttgacatag gtcagtaccg gcacgctacc catcttcaca ttcagcggtg
    44401 cagaggtttt cggcgacatc actattggcg agaaaccggc tgccggattg ccatttttct
    44461 gtgtgctggc attactgaaa atgacgtaat acggtgtcgg gttattgacg gtaaattcct
    44521 gaccgttgcg ggttaaagta accttatatt gccaggggtt tttcatgctg acgttctcca
    44581 gcgccttcgg tcgccagaac aacttaatac gcgtttgtag tgctatttgc agcgtgttgg
    44641 ctttgtttga ttttggcggg atctcacgca cgttgaagta gaacaggctt tcacgatcgg
    44701 caggcaattt gttgatatcc ggcattccct gcacttttac ctgcccgttc atcattgaat
    44761 cgatgcgttg caccggtggc agaacagtta acggtgaact gatcttgtta cccttttcat
    44821 cttcgatcca gctttgggcc agataaggca gtttcggatc gttattgcgc aacgtcacgc
    44881 tgattgattt gtcgctttca ttaaatatca aacgggtacg atctggcgtt acagaagcct
    44941 gggcagacaa actggcagcg ctaagcagta acgctagcgt cgtagcaccc aatttggcag
    45001 agcaaagtaa atcagacatg gatcattcct taatcacgtt attggttagc tgaaacagaa
    45061 acagggggat tggtgggtgt cacctgttgc gtctgctcct gaatgacagg ctttatgtca
    45121 tcaggaacaa ccggcgcgac gttgccttta tgctggcatg gcagtaacaa gccgttaaac
    45181 agatcggcag gcagcgggtc cggcaggttg atatcgcaat gcgcaacacc actccagaac
    45241 acactcatgt gttcgccagg tttcacccca gccagataaa cattgccgtc atcatcgaca
    45301 aggcccactg tctgctcgtt atcatttttt acttctgcgc caaacggagg atggctgccg
    45361 tcttgtaaac gcagcaccgc catcgctttt tgaccactga tgacggcaaa tttgcggtag
    45421 ccaatcgccc cttcagttag cgtggcttgt accaccgact gggttgcttc ggcgttttcc
    45481 ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat agttattgac gtcagaaacg
    45541 acggctttac caaacatatt ggtataaaca gcagcaccgt taccttctac cggaacatcg
    45601 gcaacgccat cggcatcaat caacaagcgt gtcccgccca tattccgggt acggtgaagt
    45661 gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg acaagcccgc agaggtgtat
    45721 tgcccttcat ggtagttcgc actgaggtca acctgcgcca gcgaaccatc atggctgtaa
    45781 tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt tcaactgata gtgagtcgcg
    45841 tcatcgacac ggctgaaata accgacctga ctgctgtccg tcccactgcc atagttaccg
    45901 ttatagctaa cggtactgtt gtcgccccac ggcatactga gcgaaatgta catgcctttg
    45961 tcggcctggt tgtcatactc gtaacggtag ccagtcatcg agatgctgac attacgaata
    46021 ctgcccatat tgaaatagtg cgagagcatg atgttgtagt ttgtctgctc ctcgcgatcc
    46081 cagtaggtat ggcgggtata gttgagataa accgaaacac ccgcatcgcg gaagttctgg
    46141 ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc gtaccattcc gctgtcactg
    46201 gcatccagat actcgctcat ggtcataaag ttctcttccg agaagcgata tccagcaaaa
    46261 gtaacgcggc tgttgagctg gtcaaagtct ttggaataac tcacacggaa ggagttaccg
    46321 tccagcgaac ctttgccata agcggtgtct ttatccagtt tggtgtgcga gtgagtaaca
    46381 tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga cgccaagcgc cgcagactga
    46441 tagttttcat ctcccagtgc gccgccatat aacgaccagc cgttagcgat cccccaggaa
    46501 gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc cccactcttg cggacggccc
    46561 atcatgatct tatagcgaac ctgacctgga cgagtgaggt atggcatcga ggcggtgctg
    46621 atgtcatatt cctgcacctg gccgttctgt tcttcaatgc gaatatgcaa cgtaccggag
    46681 acagaatcac caagatcctg aatacgaaac ggtccagccg gaacctgcgt ttcgtaaatc
    46741 acacgcccca tctggctgac ggtcactttt gctgtggtgt gtgccacgcc ggaaatgtct
    46801 ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt cagtactgac actgccacca
    46861 acatagttaa aaccatcaaa aatatcggat ctgaggtaat cctcgcccag cgccagtttg
    46921 gcttttaatg atggtaacgc ccgccaggca tagtagcgac tccactccca ttttttttga
    46981 gtttcatcgc cgctgaattc atcgtcatca ttactgcgag tatgttgata gttagtctgc
    47041 cagtcagcac gcatacgcca cggccccagg ttaaccccga ccgtcccgtt gccgctgatc
    47101 tcgttactat catcaccgcc attttcttcg tgccgggttt gtgcgttgat gctgtagtcc
    47161 gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg gatcccagtc gggataagta
    47221 tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg actggcttaa atcagcctta
    47281 atttccatgc cttccagttg accggatttc aggcatttag catcgtggct ccattgcaga
    47341 tttttcgcca cgtcttcttt taaaccaaac tgcgctacca gttccggtgt cagacaagca
    47401 tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa tatcgtactc ttccgccaat
    47461 ggctgtttat ttagttgaac ctgtaaattg tattttccgg gctcaacata tccctggctg
    47521 gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt ccagaaaacg ggaatcaaac
    47581 tgaatgtcgt cttcagccca gacactgcta taactccctg acattgccag cgcaatgcac
    47641 cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca tacacgtttc tatccgtttt
    47701 tattcttgt
//
LOCUS       DAJSKJ010000010        46616 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000010 DAJSKJ010000000
VERSION     DAJSKJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.010"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..453
                     /locus_tag="ON345_000487"
     CDS             <1..453
                     /locus_tag="ON345_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3070599.1"
                     /translation="THEGWLYLAVVVDLFSCKIIGWSMQSRMTKDIVLNALQMAVWRR
                     NPQKQVLIHSDQGSQYTSHEWQSFLKLHGTEGSMSRRDHCHDNAVAESFFQLLKRERI
                     KKKIYGTREESRSDIFDNIEMFYNSKRRRGSSDQMSPTEYENQYYQRL"
     gene            complement(561..1748)
                     /locus_tag="ON345_000488"
     CDS             complement(561..1748)
                     /locus_tag="ON345_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3070600.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLAEAREKRSEARKMLAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     ITREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIITGRADYNPAPDLASALATPKKVHFPFLTANELPHFLKD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWKDIDFETKLWEIPAEVMKIKRPHIVP
                     LSEQVITLFKQLEPISRHHPLVFIGRNDPHKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLANR
                     DDK"
     gene            complement(2048..2142)
                     /locus_tag="ON345_000489"
     tRNA            complement(2048..2142)
                     /locus_tag="ON345_000489"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2106..2108),aa:Sec,seq:tca)
     gene            2435..3817
                     /locus_tag="ON345_000490"
     CDS             2435..3817
                     /locus_tag="ON345_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR3070601.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGAMPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3827..6145
                     /gene="yicI"
                     /locus_tag="ON345_000491"
     CDS             3827..6145
                     /gene="yicI"
                     /locus_tag="ON345_000491"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR3070602.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(6198..7907)
                     /locus_tag="ON345_000492"
     CDS             complement(6198..7907)
                     /locus_tag="ON345_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR3070603.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(8028..9419)
                     /gene="xanP"
                     /locus_tag="ON345_000493"
     CDS             complement(8028..9419)
                     /gene="xanP"
                     /locus_tag="ON345_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR3070604.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9699..10904
                     /gene="gltS"
                     /locus_tag="ON345_000494"
     CDS             9699..10904
                     /gene="gltS"
                     /locus_tag="ON345_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR3070605.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10907..11773
                     /locus_tag="ON345_000495"
     CDS             10907..11773
                     /locus_tag="ON345_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070606.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11758..13839)
                     /gene="recG"
                     /locus_tag="ON345_000496"
     CDS             complement(11758..13839)
                     /gene="recG"
                     /locus_tag="ON345_000496"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR3070607.1"
                     /translation="MTGRLLDAVPLSSLTGVGAVLSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13845..14534)
                     /gene="trmH"
                     /locus_tag="ON345_000497"
     CDS             complement(13845..14534)
                     /gene="trmH"
                     /locus_tag="ON345_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR3070608.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(14541..16649)
                     /gene="spoT"
                     /locus_tag="ON345_000498"
     CDS             complement(14541..16649)
                     /gene="spoT"
                     /locus_tag="ON345_000498"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR3070609.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKDQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16668..16943)
                     /gene="rpoZ"
                     /locus_tag="ON345_000499"
     CDS             complement(16668..16943)
                     /gene="rpoZ"
                     /locus_tag="ON345_000499"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3070610.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16998..17621)
                     /gene="gmk"
                     /locus_tag="ON345_000500"
     CDS             complement(16998..17621)
                     /gene="gmk"
                     /locus_tag="ON345_000500"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR3070611.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17879..19561
                     /gene="ligB"
                     /locus_tag="ON345_000501"
     CDS             17879..19561
                     /gene="ligB"
                     /locus_tag="ON345_000501"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR3070612.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19558..20175)
                     /locus_tag="ON345_000502"
     CDS             complement(19558..20175)
                     /locus_tag="ON345_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR3070613.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(20467..21291)
                     /gene="dinD"
                     /locus_tag="ON345_000503"
     CDS             complement(20467..21291)
                     /gene="dinD"
                     /locus_tag="ON345_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR3070614.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            21730..22089
                     /locus_tag="ON345_000504"
     CDS             21730..22089
                     /locus_tag="ON345_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070615.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGHCAVMR
                     DTGGADNAISGEYMRVS"
     gene            complement(22082..22624)
                     /locus_tag="ON345_000505"
     CDS             complement(22082..22624)
                     /locus_tag="ON345_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR3070616.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSKLHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNEGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPAENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSRINDRLQAIN"
     gene            complement(23209..23559)
                     /locus_tag="ON345_000506"
     CDS             complement(23209..23559)
                     /locus_tag="ON345_000506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070617.1"
                     /translation="MKPYILAAVLISSAPALSAIHCGGFTIQPDRYRTTINGDVVNIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRLETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            complement(23556..24200)
                     /locus_tag="ON345_000507"
     CDS             complement(23556..24200)
                     /locus_tag="ON345_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070618.1"
                     /translation="MSTQPIIIEFGSCRWPRHLAIHEAGHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWQHVNYPRNVAEVKSYTLSNDDTRLRLIDQVCRDMVI
                     SLAGIVAQSRYTGEDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEA
                     LIALKWWDVIALAKILENRNHMPARNFQRIMGDIDKPVPKPMTLAELDELVSEE"
     gene            complement(24211..24672)
                     /locus_tag="ON345_000508"
     CDS             complement(24211..24672)
                     /locus_tag="ON345_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3070619.1"
                     /translation="MTAQISAYGRLVADPQTRTTANGTSMAMGRLAVALPCHAAEGGE
                     VTFWLGVVAFGKQADALAGHVKGDLVSVAGAMQINQWTGKDGGTQQGYQVVADSVISA
                     RTVRPGGKRAESVKASGASRGHATQSAPGWELYNTPDDFDQRPPFDDETPF"
     gene            complement(24669..24971)
                     /locus_tag="ON345_000509"
     CDS             complement(24669..24971)
                     /locus_tag="ON345_000509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070620.1"
                     /translation="MNTETSQKMTYQEREALKGFTDKRALQGDTQSLQMTLRMIAHWM
                     RQPAEIGFTEYATHWTAAQAGRDDGNRSTAAMAEQWPLREEMKIIPGGSDYMRKYL"
     gene            complement(25290..27731)
                     /locus_tag="ON345_000510"
     CDS             complement(25290..27731)
                     /locus_tag="ON345_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3070621.1"
                     /translation="MRNIDLIREVTSAAAGRWPYVLAGLSIDVPDSSRRHSPCPACGG
                     KDRFRFDDNGRGSFICNQCGAGDGLDLIKKVNNCDTTEAAQLAADVLGIDYRAAETDP
                     VAASHRREQLEADRQQREQERQKQAAEDAEQRRATFARLYAGMRQSATQGESDYLQSK
                     GLTGFNYPVMPDGSLLLELVDESGAVAAAQTITPQGEKRLLTGSAKRGAYHAVNAPEQ
                     PQSVLIAEGLATALSVHLMRPDALTVAAIDAGNLIHVARVMRRKHPQSQIIIAADNDH
                     QQDEQANTGKDAAEKAALSVAGWVALPPTDYKADWDDYRQQNGLEVATAAFNDSMYQI
                     QGEAVKPQLQAIAGGKSRRKAEVGDIAQMAASQKARLLSSRWDMLAVNPDSGTVYCYE
                     SGVWVKLPDSELERVMVAIFDEHEAHYTEKGIKSVVATMKLQIPVIGERSGDLIGFDN
                     GVYDLATQTFSAHSPGNWLMNHNGITYTPPAPGENITTSAPNFTRWLNHATAGDHHKA
                     ERIKAALFMVLAKRHDWQLFIEITGEGGSGKSVFSSIATTLAGEHNTASGSMMTLDTA
                     RGRAQFVGKSLIIMPDQTRYVGEGAGIKAITGGDPVEIDGKYEKQFTTVINAVVMATN
                     NEPMTFTERNGGIARRRVIFPFNNPVSDADKDPDLTAKIRREIPVIIRHLLTTFADQN
                     KAKALLLEQRDSQEALEVKRGTDPVVDMCAALFFMNEPKGMMMGGGTWAGQPEPRTYL
                     YHLYLAFMEYHGLGKPLSVNKFSRAIKSAAKEYRASYLTRSINGRAQTNVGYNEKAEE
                     FMPRAYGVESGQSDD"
     gene            complement(27724..28065)
                     /locus_tag="ON345_000511"
     CDS             complement(27724..28065)
                     /locus_tag="ON345_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070622.1"
                     /translation="MITKNFRLNALANKYATALYNHITAINGGNYFMVDAAGQQIRVE
                     IVNGVKGVRSLIDSYTLAAMKVSYPQWETVGVELLERCVTKDGLTDVGREIWQSMVKD
                     MGATVAGGNHA"
     gene            complement(28120..28383)
                     /locus_tag="ON345_000512"
     CDS             complement(28120..28383)
                     /locus_tag="ON345_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acetylcholine receptor subunit beta"
                     /protein_id="HCR3070623.1"
                     /translation="MNITTIYRHPAELEAEAMLSRKESYPDDFTLADRTAERMTRARN
                     GLAHVMTDLATQLNDEQAEIVYCWLSKVLTIVDIARIDAEGSA"
     gene            complement(28380..28694)
                     /locus_tag="ON345_000513"
     CDS             complement(28380..28694)
                     /locus_tag="ON345_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C reductase"
                     /protein_id="HCR3070624.1"
                     /translation="MSMYNDLVSCKFDGCTPDDLKGIESCASDAVDDLMLGVSAIGSL
                     MFWAAGSDGYPEESAKADMYRLGAMLGHIGEVARALNDSAANAAFLRSISEKEAKGRA
                     GK"
     gene            complement(28691..28870)
                     /locus_tag="ON345_000514"
     CDS             complement(28691..28870)
                     /locus_tag="ON345_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070625.1"
                     /translation="MLNYFRVAGCATARSGELVEFVYIVDARSAISAKAEALDQARNE
                     RLSHIQITRIREVAA"
     gene            complement(28863..29735)
                     /locus_tag="ON345_000515"
     CDS             complement(28863..29735)
                     /locus_tag="ON345_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR3070626.1"
                     /translation="MILNEPAAQKEAFNLASLLTNVNPYNLIKPKVANGCLLSIVMVN
                     DLAKLSLNLTKPDIHARGMTTNDDILNQEIITSKKLIVGNKFPLLAGGMNWIATVVNR
                     STSTFSTNTRALEDLLGVVTREGEFSESIKKSLPTIANPVYGYSAPAKSGVGIGVPVI
                     IRAHNTRHACFFVPRSYTCSMVDCMGAEQSAPVSLIPGYANPVQFTTSEIGVSGGGSQ
                     NHESEAAAMLATTPTQKPQFIWIIAAVRRDMPTITAKIHHIAAETEQEARRILARDHV
                     CFFAGRIRTGGAHA"
     gene            complement(29722..29904)
                     /locus_tag="ON345_000516"
     CDS             complement(29722..29904)
                     /locus_tag="ON345_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR3070627.1"
                     /translation="MRNQNPQPGSRYKDSHGAVVTVERVEHNRVTFYREGYQSPCVQP
                     LERFVNEFVEVRRDTE"
     gene            complement(29897..30730)
                     /locus_tag="ON345_000517"
     CDS             complement(29897..30730)
                     /locus_tag="ON345_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR3070628.1"
                     /translation="MNIEKSRLISEAAPHLNASLGTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGAAYSK
                     IKQSGRPGKDYLLSVGMAKELAMVERTEQGRAIRRYFIQCEEELQRSVPEIAARYRRQ
                     LKARISAANNFKPMCDALNMARAELGKTTQQRHYSNESNMIARIVLGGMTAKQWAQAN
                     GITGEPRDSMNAAQLEHLSYLESTNITLIELGQDYDQRKAELIRLSQRWLAKRAGVNH
                     A"
     gene            complement(30743..31177)
                     /locus_tag="ON345_000518"
     CDS             complement(30743..31177)
                     /locus_tag="ON345_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070629.1"
                     /translation="MKKKNRPVQQAANSDIRTSDITPTTSPVQVPRRTPKKHRARVYM
                     LRTGVEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSC
                     RGDVLKVITHVNHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            complement(31177..31380)
                     /locus_tag="ON345_000519"
     CDS             complement(31177..31380)
                     /locus_tag="ON345_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR3070630.1"
                     /translation="MNIETIPTKGYIRRFRLAELLGVSVSTIDRKVRDGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGEVA"
     gene            complement(31494..32447)
                     /locus_tag="ON345_000520"
     CDS             complement(31494..32447)
                     /locus_tag="ON345_000520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070631.1"
                     /translation="MTRKNDSQNDVNVWKRNNIPALEFCTLPRAADLLNCHLNDLIHF
                     IQIGAIEICLSLHDFEAALFTLGPWEESNEWEEKFPPKLMTKYTNKSPLSLFMPKAEF
                     DMNPASTVRVKRFYQNKDIPGLRKPILYLSGLWALSIGYLPSSFFGALKNNEEISFTA
                     FNLSFKEADVPLSADDFDSDDYVVMAHPLTEHLYSSGLLNEAEVKPIATITANDIFVT
                     RCQIEKVYNSIGRDIPNLINGGVSQPQSPETMIEKPVRTTAKQAELIVALLKGIGLTD
                     DDLVGSVSQLRAKANNKVNGLPFPDDDKPLIEWLRRGGISR"
     gene            complement(32575..33894)
                     /locus_tag="ON345_000521"
     CDS             complement(32575..33894)
                     /locus_tag="ON345_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3070632.1"
                     /translation="MRFGYIALGTPPIQSLDIRKMPKLTDMQIRAWIKSGERFEGRAD
                     GNGLYLRYREADKTPTWRFRYKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGY
                     DVAGEKQKRKTEALAKMEAEKNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINP
                     CIGSMKVEDVKPRHIDDMLKGIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSA
                     FEVADAGGKEVARDRWLTRDELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCA
                     ARWEEFDLDKAVWRLPEERSKNGDAIDIPLAPPAVEWLRELKTFSCNSAWVLPARKMQ
                     NRMIPHIQESTLPVALAKVRAEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNH
                     KIKGVEGIYNRHQYFDERKAALAQWCDLLVALERGEEYNVTPLRKAK"
     gene            complement(34025..34888)
                     /locus_tag="ON345_000522"
     CDS             complement(34025..34888)
                     /locus_tag="ON345_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR3070633.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            35015..35731
                     /gene="rph"
                     /locus_tag="ON345_000523"
     CDS             35015..35731
                     /gene="rph"
                     /locus_tag="ON345_000523"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR3070634.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            35797..36438
                     /gene="pyrE"
                     /locus_tag="ON345_000524"
     CDS             35797..36438
                     /gene="pyrE"
                     /locus_tag="ON345_000524"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR3070635.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(36475..37071)
                     /gene="slmA"
                     /locus_tag="ON345_000525"
     CDS             complement(36475..37071)
                     /gene="slmA"
                     /locus_tag="ON345_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR3070636.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(37178..37636)
                     /gene="dut"
                     /locus_tag="ON345_000526"
     CDS             complement(37178..37636)
                     /gene="dut"
                     /locus_tag="ON345_000526"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR3070637.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(37614..38834)
                     /gene="coaBC"
                     /locus_tag="ON345_000527"
     CDS             complement(37614..38834)
                     /gene="coaBC"
                     /locus_tag="ON345_000527"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR3070638.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            39006..39674
                     /gene="radC"
                     /locus_tag="ON345_000528"
     CDS             39006..39674
                     /gene="radC"
                     /locus_tag="ON345_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR3070639.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALIFAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            39891..40127
                     /gene="rpmB"
                     /locus_tag="ON345_000529"
     CDS             39891..40127
                     /gene="rpmB"
                     /locus_tag="ON345_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR3070640.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            40148..40315
                     /gene="rpmG"
                     /locus_tag="ON345_000530"
     CDS             40148..40315
                     /gene="rpmG"
                     /locus_tag="ON345_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3070641.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            40413..41222
                     /gene="mutM"
                     /locus_tag="ON345_000531"
     CDS             40413..41222
                     /gene="mutM"
                     /locus_tag="ON345_000531"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR3070642.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(41261..41740)
                     /gene="coaD"
                     /locus_tag="ON345_000532"
     CDS             complement(41261..41740)
                     /gene="coaD"
                     /locus_tag="ON345_000532"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR3070643.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(41748..43025)
                     /gene="waaA"
                     /locus_tag="ON345_000533"
     CDS             complement(41748..43025)
                     /gene="waaA"
                     /locus_tag="ON345_000533"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR3070644.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRNIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            43438..44496
                     /gene="rfaQ"
                     /locus_tag="ON345_000534"
     CDS             43438..44496
                     /gene="rfaQ"
                     /locus_tag="ON345_000534"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR3070645.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            44493..45617
                     /locus_tag="ON345_000535"
     CDS             44493..45617
                     /locus_tag="ON345_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3070646.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYAQSWEGE
                     CPDVFELIKAPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            45610..46407
                     /gene="rfaP"
                     /locus_tag="ON345_000536"
     CDS             45610..46407
                     /gene="rfaP"
                     /locus_tag="ON345_000536"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR3070647.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT        10766 a        11725 c        12135 g        11990 t
ORIGIN      
        1 acccacgaag gctggctgta tcttgccgtg gttgttgatc tgttctcatg caaaattatc
       61 ggctggtcaa tgcaatcccg gatgacaaag gacattgtcc tgaatgcatt gcagatggct
      121 gtatggcggc gtaatcccca aaaacaggta ttgattcact cggatcaggg cagtcagtac
      181 acgagccatg agtggcagtc tttcctgaaa ttacacggca cggagggcag catgagccgt
      241 cgcgatcact gtcatgacaa tgctgtcgca gaaagctttt tccagttgct gaagcgtgaa
      301 cggataaaga aaaagatcta cggaacgcgg gaagaatccc gcagcgatat ttttgataac
      361 atcgaaatgt tttataacag taagcgtcgg cgtggttcta gcgatcagat gtcaccgaca
      421 gaatacgaaa accagtatta tcaacggctc tgaagtgtct agattattca tggcgataca
      481 aaacaaaaaa ataaaacgaa agtaagatca tctggctagg tagccagatg atcagtgatt
      541 attgttgtat tactgcgtgc tcatttatcg tctctgttgg caagtgaatc aatgtaatca
      601 gcgtaccatt gcatcatttc acggcgtcca tcgagatatt gggcatgatt gtaagttcct
      661 ctgatagcat ttttatctac atgggccaac tgtgtttcga tccacgctga gttaaaaccc
      721 tgttcgtgta aaatggtact catggtgtgg cggaaaccgt gccctgttaa cctacctttg
      781 tatcccagta actcaataac ctgattaata ctttctttac ttataggttt atgaggatcg
      841 tttctgccga taaagaccag aggatgatgt cttgagattg gttcaagttg cttaaatagc
      901 gtaatgacct gctcagagag cggcacaata tgaggtcgct ttatcttcat tacttctgcc
      961 gggatctccc ataattttgt ctcaaaatcg atatctttcc agcgtgcgaa acgcaattct
     1021 tgtgttcgta caccggttaa cataatgatc tgagtagctg tcttagtgat tatacttccg
     1081 gtataacccg ccagatcttt gaggaagtgg ggaagttcat tagcagtaag aaagggaaaa
     1141 tgtacttttt tcggtgtagc taaagcactg gcaagatcgg gggcagggtt atagtctgcc
     1201 cgaccagtaa taattgcata gcggaacacc tcgccacagc gctggcggac cttgcgcatt
     1261 ttctccaggg ctccacgttt ttccattttt cgcaatgctt ccagtaattc cattggtttg
     1321 atttcggcaa tagggcgctt accaatatag ggaaaaatgt ctttttcaaa ggtatcaatg
     1381 atttcatcac gatagcgtaa tgaccaccga tcggcttttg actgatgcca ttcgcgagta
     1441 atagcctcaa acgtgttttt caaactcatt tgctgcgcga ttttttcttc tttttttgcc
     1501 acccctggat cgcctccagc agcgagcatt ttcctggctt cactgcgttt ttcgcgggct
     1561 tcggctagcg ttacatcggg gtatacgcca atagaaagca tcttttcttt accagcaaag
     1621 cggtacttca tgcgccagta tttagaacca ttagtgttaa tcagtagata cattccgcca
     1681 ccatcagcta gcttataggg cttttctttt ggttttgctg ttttcacttt aatatcggtt
     1741 aatgccatga cattctccgg tttttgatga atccatattc ctgaggggat ttctgccagc
     1801 aactaatctg atacccccga tgtattcctt gtataacaaa gatgttgtta gaagccgatg
     1861 ttatgtatca cattttttta attatggggg tgctgatatt tcaagagtaa atttataccc
     1921 cctaaagcac ccccaaaagt aacttgatac gggtaaagtc ggttgacttc tggatacaaa
     1981 gaaatgttga tatataaggg attagagggc gattcattga cgtcgggaga gttcagaaga
     2041 gacaagctgg cggaagatca caggagtcga acctgcccgg gaccgctggc ggccccaact
     2101 ggatttgaag tccagccgcc tcaccggaga cgacgatctt ccgcgcctcg attgctacat
     2161 ggaggcgggg cgcattatag ctacttcctt gagtttctac atcccccaga tcagtttttt
     2221 ccgcctaata cacgcagcgt attatcacga aagcattata gttcatataa aacccatatt
     2281 ttacaatgat atatgagcgg aattaacgct accacgatca cataaaacaa gaatcataaa
     2341 ttaataacca gatatcggaa tattcgctct cacagggatg gtttacaaaa tgcgttatcg
     2401 gtctcagcac ccctatagca tcaaggaaaa gcagatgaag agtgaagtgt tgtccgttaa
     2461 agagaaaatt ggttatggca tgggagacgc cgccagccac attattttcg ataacgtaat
     2521 gttatatatg atgttctttt ataccgatat ttttggcatt cctgccggtt ttgtcggaac
     2581 catgtttttg gtcgctcgtg cactggatgc gatttccgat ccttgcatgg ggttgttggc
     2641 cgatcgaacg cgctctcgct ggggtaaatt tcgtccgtgg gtactgtttg gcgcaatgcc
     2701 attcgggatc gtctgtgtac tggcctatag cacgccagat ctcagtatga acggcaaaat
     2761 gatctatgca gcaattactt acaccctact taccttactt tataccgtcg tcaatatccc
     2821 ttactgcgca ttgggtggtg taatcaccaa tgacccgact cagcgtatct cgctgcaatc
     2881 ctggcgtttt gtgctggcga ccgcgggagg catgctttct actgttctga tgatgccact
     2941 ggttaattta attggcggtg ataataaacc actcggtttc cagggcggta tcgcggtcct
     3001 ttccgtggtg gcattcatga tgctggcatt ttgtttcttc accactaaag aacgcgttga
     3061 agcaccacct acaacaacgt ctatgcggga agatttacgt gatatctggc aaaacgacca
     3121 gtggcggatt gtcggtttac taaccatttt caatatcctg gcggtgtgcg tacgcggtgg
     3181 ggcgatgatg tattacgtca catggatttt gggcacgccg gaagtgtttg tcgcttttct
     3241 caccacttat tgcgtgggta acctgattgg ttccgcactg gcaaaacctc tgaccgactg
     3301 gaaatgtaaa gtcactatct tctggtggac gaacgccctg ctggcagtga ttagcctcgc
     3361 gatgttcttt gttcccatgc aggccagcat cactatgttt gtcttcatct tcgtgattgg
     3421 tgtgttgcat caactggtga cacctatcca gtgggtaatg atgtccgata ccgtcgacta
     3481 cggcgagtgg tgcaatggta aacgcctgac cgggatcagt tttgctggca cgctgtttgt
     3541 gctcaaactg gggttggcct tcggcggcgc tcttatcggc tggatgctgg cttatggcgg
     3601 atatgatgcg gcagaaaaag cgcagaacag cgccacgatt agcatcatta ttgcgctatt
     3661 cacgattgtt ccggcgatct gttatttgct gagcgcgatt atcgctaaac gctactactc
     3721 actcacgacg cacaatctga aaaccgttat ggaacagctg gctcagggta aacgccgttg
     3781 ccagcaacaa ttcacctctc aagaagtgca gaactaagga acggcaatga aaattagcga
     3841 tggaaactgg ttgattcaac ctggcctcaa tttgattcac ccgcttcagg tgttcgaggt
     3901 tgaacagcag gataatgaaa tggtggtcta tgctgccccc cgtgatgtgc gtgaacgtac
     3961 ctggcagctt gatacgcctt tatttacgct gcgctttttc tccccacagg aaggtattgt
     4021 cggtgtgcgg attgagcatt ttcagggggc gctgaataac ggtcctcatt atccgctcaa
     4081 tattttgcag gacgtgaagg tcacaatcga aaacacagaa cgttatgctg agtttaaaag
     4141 tggcaactta agcgcgcgtg tcagcaaagg tgagttctgg tcactggatt ttctgcgcaa
     4201 cggcgaacgt attaccggta gtcaggtgaa aaataatggc tacgtgcagg acacgaataa
     4261 tcaacgcaat tatatgtttg agcggcttga tcttggcgtt ggcgaaacag tttacggtct
     4321 gggagagcgc tttactgccc tggtgcgcaa tggccagacg gtagagacct ggaaccggga
     4381 cggcggcaca agtactgaac aggcgtataa aaatatcccg ttctacatga ctaaccgcgg
     4441 ttatggggta ctggtcaatc atccccagtg tgtctctttt gaagtgggat cggagaaagt
     4501 ctccaaagtg cagttcagcg ttgagagtga atatctcgaa tactttgtta tcgacggccc
     4561 gacgccgaaa gcggtacttg atcgttatac ccgctttact ggtcgtccgg cgctgccgcc
     4621 cgcgtggtcc ttcggcctgt ggctaaccac ttcatttacc accaactacg acgaagcgac
     4681 ggtaaacagc tttatcgatg gtatggcgga acgcaatctg ccgctgcatg ttttccactt
     4741 tgactgtttc tggatgaaag ccttccagtg gtgcgatttt gagtgggacc cgctgacttt
     4801 ccctgacccg gaagggatga tccgccgcct gaaagcgaaa gggctgaaaa tctgcgtctg
     4861 gattaacccc tatatcggtc aaaaatcccc cgtctttaaa gagttacaag agaaaggcta
     4921 tttactcaaa cgcccggacg gttcgctatg gcagtgggat aaatggcagc caggtctggc
     4981 gatttatgac tttaccaatc cggatgcctg caaatggtac gccgacaaac tgaaaggtct
     5041 ggtcgcgatg ggcgttgatt gctttaagac cgactttggc gaacgtatcc caactgatgt
     5101 tcagtggttt gacggttccg atccgcagaa aatgcataac cattatgcgt acatctacaa
     5161 cgaactggtg tggaacgtgc tcaaggacac cgttggtgag gaagaagctg tcttgtttgc
     5221 ccgctcggcc tccgtcggtg cgcagaaatt cccggtacac tggggtggcg attgttacgc
     5281 taactacgaa tcaatggcgg aaagcctgcg cggtggtttg tctattggcc tttcaggttt
     5341 tggcttctgg agccacgata tcggcggctt tgaaaatacc gctccggcgc acgtttacaa
     5401 acgctggtgc gcgtttggtt tgctctccag ccatagccgt ttacacggta gcaaatctta
     5461 tcgtgtgccg tgggcctacg atgatgagtc ctgtgatgtg gtgcgcttct tcacgcaact
     5521 gaaatgccgc atgatgccgt atctgtatcg tgaagctgcg cgtgcgaacg cgcggggtac
     5581 gccgatgatg cgggccatga tgatggagtt cccggacgat ccggcttgtg attaccttga
     5641 ccgtcaatac atgttaggcg acaacgtgat ggttgcgccg gtgttcactg aagcgggcga
     5701 tgtgcagttc tacctgccgg aaggtcgctg gacacacctg tggcacaacg atgaactcga
     5761 cggtagtcgc tggcataaac agcagcacgg cttcctgagt ctgcccgttt atgtgcgtga
     5821 taacactcta ctggcgctgg gcaacaacga tcaacgtccc gattacgtgt ggcacgaagg
     5881 cacggcattc cacctcttta atctgcaaga cgggcatgaa gccgtctgtg aagtgcccgc
     5941 tgctgacgga tcggtgatct ttactttaaa agcagcacgt actggcaaca cgattactgt
     6001 gactggtgcg ggcgaggcga agaactggac actgtgcctg cgcaatgttg tgaaagtaaa
     6061 tggtctgcaa gacggttcgc aggctgaaag tgagcagggg ctggtggtga agcctcaagg
     6121 gaatgcgctg acaattacgt tgtaatctat tgatggaatg ccgggtacgt ctgagtcgta
     6181 cccggcgaga ttgatgttta ccgcaccggc tccgtcgatg taacttcacc gctggaaacg
     6241 atgccagcat tcatggtttg agtcacttgc tgctttgcgg cgttcacggc atgcaattgc
     6301 ccgctaaccg taggtttcaa cgggacattg gcctgaatat cgccactggc ggtaagctga
     6361 atattgccgt cgccagtcaa cggtaattta ggccatcccc actgttgcaa aatattcacc
     6421 ggcacaccgc gtccattcag gctgatatgt gtctgacgtt gtggcgtttg tgaaacactg
     6481 gcggtggctt ccagaatgcc tttttcagta aatgcactca gttcgctgat attcaccgtg
     6541 ctgctgttgg cggtcagcgc cagcgacggg cgacgaacat caacacgatt gaatgtcgcg
     6601 gcggcggcat tcagattcgc cgagccactc cagacgcccc atttatgatc ggtaaccagc
     6661 gtcaggttgg caccgtaacc atcgagcgcg gtgagctgcc acgggaagtc agggtcgata
     6721 tcaatgatca gattgcggct ggcgctaaat ctcttcagtt gcaggctgtt taaccaaccg
     6781 ggtgtcgttt ccatccacaa ctgttgccag tttttcggca aggtatattc cagcccggca
     6841 attgccgcat catcaaggat caacgttttc ccgtcacgca gccagttccc tgacgttctg
     6901 accatacccc cttcccagcg gctggtgaac tggcgcagcg ctacgccctg cggggaaaat
     6961 tccgcgttta taatcgggtc aaataaatgc agcgaaccat aaatgaactc gctagcgttc
     7021 atcgacagtt tgccttcctg tgtctgccag tcatctttac tgaaggtcat gttgcgcaag
     7081 ctgagatcga ggtcggtcac cgcccagtcc ggaccttgca aacgagcatc gatcacttcc
     7141 aggcgaccaa tctgcaacga cgggacagag cgtaatggcg caaagaagtc ggttagcgat
     7201 ttttcgcttt gtagacgtat atccgccatg cgcaggtttt ccacttgcca gctgccgtcg
     7261 gcgttacgct gcgcgtttcc ggttaatgtc ccgcgggcga tgtcggcacc caggttagtc
     7321 agcgtaacgc gatcgttatc aatactgcct tcaatcagta cattggtggc aggaacatca
     7381 ttgagcgaaa gcgatccggc actaaactga atctgcgcct tcgtacccag cactttaccg
     7441 gcttttggtg accacggaac tacgccgcca tttacccgct gcgcgctcag tttccattcg
     7501 ctattcgggc tattaaacgc catatcacgc agttgcagac gatcggcttt gaacggtagc
     7561 ggcgcggtct ggtcggtgag attcagcgtc ccgttttcca gcaggatggt atcgacatgg
     7621 cgtggttcgg tcagttgccg actgcttagc gcaatgtcga cacttttggc caccagggtc
     7681 gcgggctggc catcacgacc aaacgtgacg ttctccagca cgatatgaga tggcgcggaa
     7741 aaacggtgat ccatcgcccc gaaggccaga tgatagtcgc tattctcgga aacccatgcg
     7801 ctgatatgtt ccgctcccca gcgggtttgc agaagaaaat agaggccagc gatcgccact
     7861 aacagagcga tgagaatgta gagaagcagc ttcccaataa atttcatggt cttccatccc
     7921 gtgaaatgca cataagggag ttatgcacga tttacgcgca atcctcaagg cgggaatggt
     7981 taaagaggtg agactgcggc ggtaattatc ttaccgccgc cagtgaatta ctgtttttct
     8041 ggtgggaaaa tcagattcag cacgatggca gtaataccgc ccgcggcgat cccggaggag
     8101 agcaggtttt tcagccattc aggggcaaac tgcaaaatca gcggctgctg agacacgccc
     8161 agaccaaccg ccagcgacag cgcgataatc agaatcgccc gacggttcag cggctcacga
     8221 gaaacgatac gcacaccgga ggcggcgatg gtgccaaaca ttacaagcgt tgcgccgccc
     8281 agaaccggtt ctggaatgtg ttgtacaaaa ccgctcactg ccgggaacag acccagcacg
     8341 atcaacatca gcgcgacgac aaaaccgaca tagcggctgg caacaccagt caactggatc
     8401 actccgttgt tttgcccgaa gcaggagttc gggaaggtgt taaacaccgc cgaaacaaac
     8461 gagttcaggc cgtttgccag cacgccgcct ttcaggcgtt tcatgtacag cggaccggac
     8521 actggctgtt cggaaacgtc agaggtcgcc gtgatatcgc caatggtttc cagcgaagtg
     8581 atcataaaga ccagcatcag cggcagcagc agactccatt caatgccaag accgtaatag
     8641 agcggcgttg gcaccataat cagttcttgc gtcatcggtt cgttgctttc tggcaacatg
     8701 cccataaacc acgccagcgc atatccggcc gccatcgcaa ttaccagtga ggccacgcgt
     8761 aagtaagggt tacgttgacg gttaagcagg ataattaagg ctaaaaccac gcctgccagc
     8821 agcagatttt tcggtgcgcc gaaggtgtta tcgctcatgg ctgcgtaacc gccgccaatg
     8881 gaggtcagcc ccacctgaat tagcgacagg ccgataatca tcaccacaac gccagaaacc
     8941 agcggcgtaa taatgcggcg cgccagatgc agaacgcggg agatcaccat ctcggtgcaa
     9001 cttgccagca tcaacgtgcc gaacaaagcc gccatcatgg taggaacatc agcaccaccg
     9061 gttttcagcg cggtaccgcc cataatcagc ggggcaacaa agttgaagct ggtgccctga
     9121 atagacaaca gcccggagcc aaccggaccc caggccttaa tttgaataat cgatgccaca
     9181 ccggaggcaa acagcgacat actaataatg tgttgcgtgt cttgtgccgg taaacccagc
     9241 gcctggcaga ttaatagcgc tggcgtgatc accgcaacga acatcgccag cagatgctga
     9301 caggcggcaa acagggtttg aggaagcggc ggacgatctt caagacggta aatcagttcg
     9361 ctgttttgag tctgcgcaac cggttgcgca ttttctgact cgagggtgga aacagacatc
     9421 gaaaacaatc ccgtggtagc aaagcgggca ttttagctga ccgaaagaga aaagcaaacg
     9481 tttgccacat cctcatctaa atatttaaat gagataaatg cactttttta tataactttt
     9541 ggtatttttc tgcttaaaat ccatgccatt tgttcattaa cagctgagaa tttgatccag
     9601 gaaagcataa ctcgataccc gtcatagttc atgaggttgc cactgcgcga tgaagtatga
     9661 cgagtatgaa agagtgatgc ggatacaaag gagtaactat gtttcatctc gatactttag
     9721 caacgcttgt tgccgcaacg ctgacgttgc tgctcgggcg taagttggtc cattccgtct
     9781 cctttttgaa gaaatacacc ataccggaac ctgttgcggg tggtttgttg gtggcgctgg
     9841 cgctactagt actgaaaaaa agcatgggct gggaagtcaa ctttgatatg tccctgcgcg
     9901 atccgttaat gctggctttc ttcgccacca ttggcctgaa cgccaacatt gccagtttgc
     9961 gtgccggtgg gcgtgtggtt ggcatcttct tgattgtggt tgttggtctg ttggtgatgc
    10021 aaaatgccat tggcattggt atggctagcc tgttagggct tgatccgctg atggggctgt
    10081 tggccggttc tattacgctt tccggcggtc acggtacggg cgctgcgtgg agtaaattgt
    10141 tcattgaacg ttatggcttc accaatgcga cggaagtggc gatggcctgt gcaacgttcg
    10201 gtctggtgct gggcggcttg attggcggtc cggtggcgcg ctatctggtg aaacactcca
    10261 ccacgccgaa cggtattccg gatgaccagg aagtcccgac ggcgtttgaa aagccggatg
    10321 tgggacgcat gatcacctcg ttggtgctga ttgaaactat cgcgctgatt gctatctgcc
    10381 tgacggtggg gaaaattgtt gcgcaacttt tggctggcac tgcttttgaa ctgccgacct
    10441 tcgtctgtgt actgtttgtt ggcgtgattc tgagcaacgg tctgtcaata atgggctttt
    10501 accgcgtctt tgagcgtgcg gtatccgtgc tgggtaacgt aagcttgtcg ttgttcctgg
    10561 cgatggcgtt gatggggctg aaactgtggg agctggcttc gctggcgctg ccgatgctgg
    10621 cgattctggt ggtacagacc atcttcatgg cgttgtatgc catcttcgtt acctggcgca
    10681 tgatgggcaa aaactacgat gcggcagtgc tggctgcggg tcactgtggt tttggcctcg
    10741 gtgcaacgcc aacggcaatc gccaacatgc aggcgatcac tgaacgcttt ggcccgtcgc
    10801 acatggcgtt tttggtggtg ccgatggtcg gtgcgttctt tatcgatatc gtcaatgcgc
    10861 tggtgattaa gctgtattta atgttgccga tttttgccgg ttaaaaatga aaaagcgtcg
    10921 gaaggaaccg gaaacgctac gtgaacactg tcgccatatt tttggtgatg agccgccagt
    10981 gctctgtgta tgggaaacgg aatttgatta cgcagatgcc gaactcaagg cgctggcggc
    11041 taaagagtgg cagcagatca gcgaatggga tttgagtgct tactatgtac tcaatctcgt
    11101 ttataacgaa ccgatgcaaa tagagttgtt tcgctatctg tttccgctct gcctggcgca
    11161 atggcatgaa accgttttag ctggcggtta tggtgatcac ttcgaagaat ccttaatgaa
    11221 ggcattgtgt cggccctatc tctggcagga gatgatgaat gcttcccaac gccagcaggt
    11281 acggcaattt ttactggaca ccgcgttgca gcgtatggat aacgagcgtg gtttcaataa
    11341 cgttctttgc tggctggcgg tgttcaatac tctgggtggg gccgcgccgt taattcgttc
    11401 tctctggtcg cgctggtggg cgctggatac gccaggaaaa gcggtgtgtg ctattcagta
    11461 tgccgcgcat ctaatttatc ccatagaagc caatccgtta tggtcgcaag aatggatagg
    11521 ctgggggcat ccattaggcc ataaagatgg ctggtcctct gataatcgcg cttttcttcg
    11581 ccaaatgtta acacctgaaa tgattgtggc tggcgtacag gcagcggctg aaattctgcg
    11641 tggtgaaccg gaaggcgcaa tggcggcgcg aattgcacag gatgcgtatg aggcaatgga
    11701 tatcctcact atccagattg aagatttact gcgtgacttg tcttgtgatg agtctggtca
    11761 tgcgttggag taacgttccg tctccggcat ccagcgttct atcagggctt ttgcttgttg
    11821 tgggtaacgt tcgtgaatat gtcgcgccag gcgctgaact tccgggatca tcgcctgatc
    11881 gcgcagtaaa tccgccactt taaactcagc attacccgtc tgacgcgtac ctaacagttc
    11941 acccggtccg cgaatctcta agtctttctg tgcaatcaca aaaccgtcgt tgctgtcgcg
    12001 cagcacttgc agccgaattt gcgcggtttt ggagagcggc gttttgtaga gcagcacgca
    12061 gtgagaagcc accgcaccac gacctacacg cccgcgaagc tggtgtaact gcgccagacc
    12121 cagacgctca gggttttcga taatcatcag gctggcgtta ggcacatcaa caccgacttc
    12181 aataacggta gtggcaacca gcagatgtag ctcaccttgt ttaaacgacg ccatcaccgc
    12241 ctgtttctcg gctggtttca tccgcccgtg taccaggcca acattcaatt ctggtagcgc
    12301 cagtttcaac tcttcccagg tagcttccgc cgcctgcgct tccagtaact ctgactcttc
    12361 aatcaacgta caaacccagt acgcctgacg accttcagtt atgcaggcgt ggcgcacgcg
    12421 gtcaatgatg tcggtacggc gggtatcagg aatagcgacc gtagtcactg gcgtgcggcc
    12481 tggcggcagc tcatctatca ccgaggtatc gagatcggca tacgccgtca ttgccagcgt
    12541 gcgggggatc ggcgtggcgg tcatgatcaa ctgatgcgga tggaagcctt gctgctggcc
    12601 tttctcccat aacgccagac gctgatgcac gccaaagcga tgttgttcgt cgataatcac
    12661 cagcgccaga ccgttaaact gcacctgttc ctggaagatg gcgtgcgtac caacaatcat
    12721 ctgcacctga ccgctggcga tggcttcctg ctgagacagt cgcgctttgc ctttctgctt
    12781 accggcgagc cagcccactt cgataccgag cggttcaaac cagttgcgga agttattggc
    12841 gtgctgctcg gcgagtaatt cggttggtgc catcaatgct acctgtttgc cgtgggcaat
    12901 cgcacgcaac gcggcgaggg cggcgaccag cgttttaccg gaacctacat cgccctgcac
    12961 cagacgcatc atcggcacat ccagcgccat atcgcgctcg atctccgcca ctacgcgtgc
    13021 ctgtgcgccc gttggcttga acggtaaggc ggcgaggagt ttatttttca gcgtgtcatt
    13081 ggcgctcagc ggctgggcat gaaaacgctg tgctccggca cgtaaggcta acatgctgag
    13141 gttgtgcgcc agcagttctt ccagaatcag acgacgttgc gccggatgct gcccggtttc
    13201 cagatcgcta agctgtagcg tcggtggcgg gcggtgcaaa gtgcgcaacg cttccggtag
    13261 cgtcatcatt ccttgtgaca gttccggcgg caggagttct tcaatggcgc aggtgtcgag
    13321 cagatccagc gcctggtcgg ttaatttacg cagcgtggcc tgctttacgc cttccgttgt
    13381 tggataaacc ggcgtgagcg tttcctgtaa ttctggcgtg ctgagatcgc cctgcacgcg
    13441 gtattccggg tggatcatct ccgcaccata tttaccgcgc tttgcttcgc cataagccag
    13501 tacacggcgg cccgccgcca ggctattttt cattgccgcg ctgaaattga aaaagcgcat
    13561 ggtgaggatg ccggaaccgt cgctgatctg gcaggtcatc atccgccgac cgccgaagga
    13621 gatattgcag ttcagcactt cgccttccac cgtggcataa acgcccggca gtagttctcc
    13681 gatggggtag agatgggtgc gatcttcgta gcgcagggga aggtgtaaga gtagatcctg
    13741 cacggtatgc aggttgattt tcgccagttt gttactaagt actgcgccaa cgcccgttag
    13801 ggaactgagt gggacagcat ctaacaggcg acctgtcatg gcacttaccc tgcagcctgc
    13861 atagtagccc accagtcggc atcagcttcg atctcgcctt gctgattgac gtggggataa
    13921 ggcaggcctt tgcgttttgc gactttcgcc agcaccggat agccgccttc aaacaacagg
    13981 cgttgttgct ctgcttccgg caacatgctg ttttcacgca ggtacatgcc tgcattttgc
    14041 cgctgacgct gggcttcgta aagaatgagg gctgaggcaa cggaaacatt cagcgactgc
    14101 accatgccga tcatcggaat gatgatgtcc tgatccgcca gggccaatgc ttcctgcgtg
    14161 atgcccgttt tctcctgtcc catcaaaatg caggtcgggc gagtgtaatc aatttcgcgg
    14221 aaatcgacag cgttatcaga aagatgggtt gccagaatct gcatgccctg gcctttgaga
    14281 tgagcgacgg catcgccaat ggtgcggtgt gttttcacct gtacccagct gttactaccc
    14341 gccgctgccg aagccatggt gcgcatgcgg ctaccaggcc agacggcgtg aacttcatgt
    14401 acgccaacgg catctgcggt acgaataatc gcagaaacgt tatgaggttt gtggacctgc
    14461 tccatgcaga cggtcagatc aggctgccgc ctggcgagca tttcgcagat gcgtgcataa
    14521 cgtgttgggt tcataaaaca ttaatttcgg tttcgggtga ctttaatcac gtctggcatc
    14581 acgcggattt tgcgcatgat attcgccaga tgcacacggt cacgagcggt cagacgaata
    14641 aaggcgctgt agacgcgacc atctttctct tccgtattca aactttgaat attcgaggtc
    14701 gtggtgttaa ttgccgccgt caggtttgcc agcgcaccct gatgattgaa catctccacc
    14761 ttgatttcgg tgatgaactc ctgcgccgtc tctttatccc attccacagc cataaacttc
    14821 tctggctctt tctggtagcc acggatgtta cggcaggatt catggtggat caccagacct
    14881 ttaccggggc tgacgtgggc gataatcggg tcgccaggaa tggggcggca gcatttcgca
    14941 aaggtgatca gcacgccatc agcgcctttg atcggcagat gcccgtggct ttgagtcgcc
    15001 ggtggaatgg aggcgtcccc atgttgtaga tttttcgcga ccaccacgct cattgcgtta
    15061 ccaaggccga tttctgccag cagatcgtca agcgttgcca gcttcatgcg atccagctcg
    15121 cgctgaatat tttcctgcgg gatttcattg agcttacggc taccacccaa agcatggttg
    15181 agcagacgac ggcccaggct tacagaatca tcacgcttga ggtttttcag caactgacga
    15241 attttggcgc gcgctttcga gctaacgaca aagttcagcc aagcggcatt cgggcgagcg
    15301 cccggagcgg taatgatttc aacggtctga ccgctagtaa gcggctgcga cagcgggtaa
    15361 ggctggcggt cgacgcgtgc gcccacgcag gcatggccga tatcggtatg cactgcataa
    15421 gcgaagtcga cgggcgttgc accggcaggc agctcgacaa tgcgcccttc cggtgtgaaa
    15481 acgtaaatct catccgggaa gagatcggat ttaacgctct cgataaattc aaacgaacta
    15541 ccggcgctct gttgcagctc cagcaggctt tgcatccagc gctgggcgcg gatttgtgcg
    15601 gtagtactgg tttcgccgtg ctctttataa gcccagtgcg cggcaacacc catctccgcc
    15661 atctgatcca tatcttcggt acggatctgg acctcaaccg gcacgccgtg cgggccaatc
    15721 atcgaggtgt gcaacgactg atagccgttc gctttaggaa tggcgatata gtctttcacg
    15781 cgacccggac gcggcttgta caggctatgc atttgcccga gcacgcgata acaggtgtca
    15841 gaatcattga cgatcacgcg gaaagcgtag atgtccatga ttgagtgaaa acgctgatct
    15901 ttgagcacca ttttgcagta aatcgaataa agatgctttt cgcgaccact gacgcggcac
    15961 ggtattcccg cttcctgcaa acgcccttcg atttcagaga ggattttttg gatcatctct
    16021 ttacggttgc cgcgcgcggc tttcaccact tctttaatta cgcggtaacg attgggatac
    16081 agcgcctcaa aacccagctc ttcgagttcg gttttaatgt ggtggatacc taaacggtgc
    16141 gccagcgggc tataaatttc gagagtttca cgggcgatgc ggcgacgttt gtccgggcga
    16201 agtgagccca gcgtgcgcat gttgtgggta cggtcggcaa gtttgatgag aatgacgcgg
    16261 atatcctgca ccatcgccat aatcatcttg cgaaagtttt cggcctgcgc ctctttctta
    16321 tcgcggaact tgagcttatc aagtttcgac accccctcta ccagctcggc gacgctttta
    16381 ccaaaaagct gttccatgtc ctggtaggtg gcgggagtat cttcaatcac gtcatgtagc
    16441 agcgctgcca tcagcgtttc atagtcgagt ttcatctcgg ccagaatgca ggcaaccgct
    16501 accgggtgcg tgatataggg ttcaccgctt gaacgtgttt gcccctcgtg agcatcacgt
    16561 gcaacgagat acgcctgccg cagacgcttg atttggtctt ccggcaggta ggtttgaatc
    16621 agttgattca ggctttcaaa cagatacaag ggcgacccgc tttgtgatta acgacgacct
    16681 tcagcaatag cggtaacggc ttgtaattca gcggcttcct gctcttgctg ttcctggcgt
    16741 tcgcgaacgt cgaggatctg gttgttgatc agaccttctt cgatttcgcg cagcgcgatt
    16801 acagtggttt tatcgttttc ttccggtacc agcggatcct ttccgcctac ctgcatctga
    16861 cgagcgcgac gcgcggcgac cagtaccagg tcaaaacggt taccaatttt ctctacagcg
    16921 tcctgaacag ttacgcgtgc catacttaaa aagctccaca ggtgaagaaa tgactgggca
    16981 tgatactgaa atcaggttca gtctgccaac aatttgctga ttaaagcgtc atgacgctgc
    17041 ttttggcggc tcatgcgcag acgttcggcg cgaataatgg tcttcaaatc ggtcaacgcg
    17101 gtatcgaagt catcattcac aatcagataa tcatattcgg cgtaatggct catttctgca
    17161 acagcttgcg ccatacgctt tgcaatgacc tcttcgctgt cctgaccgcg accgcgtaga
    17221 cggcggtcca gttcaatttt ggacggcggt aaaataaaga tactccgcgc gtgcggcatt
    17281 ttctggcgaa tttgctgcgc gccctgccag tcgatatcga gaaaaacatc gacaccggtc
    17341 gccagtactt gctcaatggc ctcacgcgaa gtgccatagt aattaccaaa aacttctgcg
    17401 tgttcgagga acgcatctct gctaatcatt tctttaaatt catcatgatt aacaaagaaa
    17461 taatgttcac cgtggacttc accaggacgc ggttggcgtg tggtgtgtga aacagaaacc
    17521 tgggtgtcat acaacggttg ggtttttaat aaagcctgaa tcaggctgga tttacccgcg
    17581 ccactggggg cagaaacaat ataaagcgtg ccttgagcca tgagtatctt tcgtatgtga
    17641 ttagcgaaat aaagcctaca tacgagctta ttatacacgg cgcggccatg tgacgtagcc
    17701 cttgctcaca ctttttgcgc aaagttgttg cgttttgcgt agctgtttcc gcatttgcct
    17761 gtgatttact gcaataaaga cgattctcag gaagcgtcct cgcagactcc gtttttgcca
    17821 ctcggctttg ccttcaccac gttgtatatc tgccagcctg actacagcgg aggtgaagat
    17881 gaaagtatgg atggcgatat taataagtat cttgtgctgg caatcatctg tgtgggcggt
    17941 ctgtccggcc tggtcgccag ccagagcaca ggaagaaatt ttccgcctgc aacagcaaat
    18001 aaaacagtgg gacgatgact actggaagga aggaaaaagt gaggtggaag acggtgttta
    18061 cgatcagtta agcgcccgtc ttacgcagtg gcaacgctgc tttgtgagcg agccccgcga
    18121 cgtgatgatg ccgcctttaa atggtgcggt tatgcatccg gttgcccata cgggtgtacg
    18181 caaaatggcg gataaaaacg cattaagtct gtggatgcga gaacgtagcg atctttgggt
    18241 gcagccaaaa gttgatggcg ttgcggtaac cctagtttat cgggacggga aactgaacaa
    18301 agcaatcagt cgcggtaatg gcctgaaagg cgaggactgg acgcagaaag ttagcttaat
    18361 ttccgctgtg ccgcaaaccg ttagcgggcc tttagccaac agtacgcttc agggggaaat
    18421 atttctccag cgcgaggggc atatccaaca acaaatgggg ggaataaatg cccgcgcaaa
    18481 agttgctggc ctgatgatgc gccaggacga tagcgacacg ctgaattctc tgggcgtttt
    18541 tgtctgggca tggccggatg gaccgcagtt aatgaccgat cgtttaaagg agctggctac
    18601 cgcaggtttt actctaacgc agaggtatac ccgtgcggtt aaaaatgctg atgaggttgc
    18661 gcgcgtacgc aatgagtggt ggaaagcgaa attacccttc gtcaccgatg gcgtggttgt
    18721 gcgaggggcg aaagaacccg aatcccgcca ttggctacca ggccaggcag aatggctggt
    18781 ggcctggaaa tatcaacctg tagctcaggt tgccgaagtg aaggcgattc agtttgcggt
    18841 gggtaagagc ggtaaaatat cggtggttgc gtcactcgca cctgtcatgc tggatgataa
    18901 aaaagtccag cgggtgaata ttggttccgt caggcgctgg caggagtggg atattgcgcc
    18961 tggtgatcag atcctcgtca gccttgccgg tcagggtatt cctcgcattg atgatgtggt
    19021 ctggcgcggt gcagaacgta caaaaccgac accaccagaa aaccgcttta actcgttgac
    19081 ctgctacttt gcttctgatg tttgtcagga acagttcatt tcacgcttag tctggctggg
    19141 gtcaaaacag gttctggggc tggatggcat tggtgaggcc ggctggcgcg cgctgcatca
    19201 gactcatcgc tttgagcata tcttttcctg gcttttatta acgccagagc aattacagaa
    19261 cacgccgggg atcgcgaaaa gtaaaagtgc gcagctatgg catcagttta atctggctcg
    19321 taagcagcct tttactcgct gggtgatggc aatgggaata ccgctaaccc gggcggcgct
    19381 taatgccagt gatgaacggt cctggtcgca acttttattt agcacggagc agttctggca
    19441 gcagctgccg gggactggat cggggcgcgc cagacaggtt attgaatgga aggaaaatgc
    19501 gcaaatcaag aagctgggca gttggctggc tgcccagcag atcacaggtt ttgaacctta
    19561 gtgtccttcg tggctgtagt aaaagaccgg taatcccagc ttaaggcgta gcgccagtaa
    19621 gcgggcaaaa aagccgaata ccagggtgga gataattacc acatcatggt tagaaacata
    19681 gtgttgcagc gcgatgtaca gcacggcgga ggcaaatgag acaccggcat acaactcttt
    19741 ctggaatacc agtgggatac gtttacagaa catatcgcgc aaaacgccgc caaacacgcc
    19801 ggtggttacc gccgcgacaa cggcaataat tgggccgtgc cccatatcca gtgcaacctg
    19861 tgcgccgatg atagaaaaga ccaccagtcc aagcgcatcg agcaccagaa acactttgcg
    19921 caggtaaggc attacaggag caacgatagt ggtgagtact gcggcggtag cgacgataat
    19981 cacatattcc gggtgtttga cccagccgag cggatagtgg cccagcagaa tatcgcgcac
    20041 tgaccctccg ccaattgcgg tggcggtcgc aataataatt acgccaaatg tatccatgcg
    20101 gcgtcgcccg gccgccagag cccccgtcat ggcttcggca gtaatgccaa ccaaatacaa
    20161 aatgtgtaac agcatggttc tctccctaaa aatctgtctg gcagggtagc gatttgtgcg
    20221 tattgtcacg attgagattt tttaaggtga gaggaaattg ataacttgta gttatgtgaa
    20281 atcgctattt tctgtagcgt agatgcattc ttctgacttc ttgtctgaga aaatgcataa
    20341 gaaaaaatag gttaaaattg aggggtggaa aagaaaggtg caatgtttag ccattgtgtt
    20401 caccgcacct ggactttggc ttacgcatcg ggtatctcaa tcaacaaaaa gagtttttag
    20461 tttctgctat ttcttctctg taatttttac actgttttcc aactgcttga tgctgacctg
    20521 cggggtcggc aactcctcag gcatggttcc gccaagttcc tgaatggttt gcctcacctt
    20581 gctacccacg tcaaagtgtg tggtattagc ttgctgtttt gaattcacgc catcccgctt
    20641 gagtttttct tctgtttggg tagctcgaaa gagattagcc gccagttctg ttgaacccat
    20701 atgatccagg atcttctgac tctttttcag ccccttccgc tgatggatgg ctttctgatc
    20761 taatccacca tacagccccc ggtaaccatg attctgaaat atggcgaaat cgatagctgt
    20821 cgctacccca gcttgctgcg ctgcctcaac caattgtttg ttatgttctt tcaattcatt
    20881 tctcagaaaa agacgctttt cgtcttcacg aagttgcctg aaagcttcat cgtcagccag
    20941 ctcctgccgt cgggtctgga tagcaaaata agtttgccct gccgcaataa ccggtttcgc
    21001 agggtcgccg ttttgtacta ccaggtaaca ggcatagcgg gagagatgaa catcttcaag
    21061 ttctcgttga gcccctgaac ctaatacaac cattttggtg gtttcaacga aatggtcaga
    21121 tgcagcttga ttgctggctt cgcacgcttg agtcgcgcgg gccagtacct tttgaaaatt
    21181 gcgccagtcc cgataatcca gtaacggtgc caattctcta gctgaccaaa attcttgccc
    21241 ttcagtgcca taatgccgta tttcttcaaa aggttgatga tgttcgttca ttgcccactc
    21301 catgtgatat cgattgcaaa gaaagtaaat ctgtaactgt atttatatac agttgtgagt
    21361 atctcacgtt ctcagtgtgc tgggcaaaaa aacatcgcaa aatctggagc aggcacgcaa
    21421 aacagaattg aagttgcggg gaagaaaaat gccgctctgt ttgagtgggc ggcatttgtg
    21481 aaggctgaaa agtaagaatt agtgttacga gttactctgg atctgtttat tgagtagtgg
    21541 ctttaaactc ccgtggttgc tgtatttctt gtaatgcgtt ttctcagtag gtacaccctt
    21601 gtgtacacgg tattttgagg tgctgccttt ggttgctcat tttatgctct cgtgcttgct
    21661 gttctggcct cttatctttt gcgccttatt gtatgatacc gctatcccac aataaggaat
    21721 cagccagcca tgagaatgac cagccgtaaa aaagagatac tcagttattt tgagccggat
    21781 aacctcgaat gggtgactgg tgagattggt gcgccgccgt tcgatgtgtc gggtgtggcc
    21841 tacctgcttc atggcatggt gtcgttcgat aagcgtcacc agctcgaatc aacccggcgc
    21901 acactggaaa gcatggttgc tggtggactg cttgagcggg tgacagtgta tgaatcgcgc
    21961 cagatacggc gcggcggtga gactaacgca acggtcgtgc gttacggttt acccggtcat
    22021 tgtgctgtta tgcgcgatac tggcggcgct gataacgcaa tatcaggtga atacatgcgg
    22081 gtcagttaat agcctgcaat ctgtcgttga tgcggctcag gtcgtaaacc cggcgttcaa
    22141 tatccagcat caggaaattg tggatctcgg cctgctcagg attatcagac tctctggcct
    22201 gtaatagcaa ttcattgtaa ccatgcaagc cgcctgagtt ttccgccggg gtggtgcctt
    22261 tcccacgcag gcagctaatg ggctgattac tcgtatcgtg tgtttgcttc tccagcacaa
    22321 tgacgcaatc ccaccagtcg ccaaagtcat acgtgtaaac cagcttgtca ccctcattct
    22381 ggagcgccgc ccatagtggc gtgattgccg cgttacccat gctttcatcc tcaccccata
    22441 cgcgatactc gctaccggga tcacctttac gccctttctc gaacatatac aggtgttcgt
    22501 cattccagcc aaaagctaac tggatcacgg catgaagttt actaaagggg atttgctctg
    22561 gaaccagcac acgccgccat ataggtggtt tgctgtctcg gatggttatc tttagctggt
    22621 acatgcaaat cctgaataaa tgcgaatcgg aaggataacg atactgacgt actcacaatt
    22681 ctgtaaaggt gaataaagga aatttgctta atacgcataa gttacatgtg gaataatccc
    22741 atatctacct gcgcggcgct ggcttcttcc actggtaggc aggtgctcgc atcatttcgc
    22801 ggaacctgtc gcgccgggct atatcggcat taactgagcg ttctatctcc cgttgatctg
    22861 cctgtgtaag ctggtggcct ccgtgctcaa gtttggcgat ggtcaataag gctatttcgc
    22921 gtggtgtcat acgtcatacc ctctggaagg atgaaacacg cccggattaa atttcatttt
    22981 tcgcgggtat aaaaaaacga tggggcgggc agtccttaag gctcaggatt gcagagattt
    23041 ggcctccccc tcccggtgat agtttattgc ttgatgtgga cggcttgcta tattggcggc
    23101 gaatgcaaat tgtcgcagaa gtctatcccg ttctctgacg ccctgtgtgg ttgtggtgcg
    23161 aatcgcccgc gtcacgccgt tgattttagg ctgcgctcgg aacgaacatc acggcgcaga
    23221 agtgtaccaa ccgaaacttg ttaacccggc atcgcagcgc tcacgattaa gcacacgtgg
    23281 cgggttttct ctggtcatca actcaagcaa ggcattgcct ccctgcgtct ccagtcggta
    23341 ttgcctatcg gtagtggtaa tggcatccgg caataacttt atggcggcct ggctaaagtc
    23401 tcgcggtgcg ccgtaaaatt gcgcatcaac aatattaacc acatcgccgt taatcgttgt
    23461 ccgataacgg tctggctgta tggtaaagcc cccacaatgg atagcagata gtgccggtgc
    23521 cgatgagatc aggacggctg ccagaatgta tggcttcatt cttcactcac caattcatcc
    23581 agttcagcca gcgtcatagg ctttggtaca ggcttgtcaa tatcacccat aatgcgctgg
    23641 aagtttctgg caggcatatg gttacggttc tccagtattt tcgccagcgc gataacatcc
    23701 caccatttca gggcaatcag cgcctcagca cgggaaaggg atattctctg tatatcagca
    23761 tcctctccac cagccacccg gtatacctct gataaatcac gaacattttt catgtctgct
    23821 ttcccggacg tttccatcag tgcgttaata tcttcccccg tatagcgtga ttgcgcgacg
    23881 ataccagcca gagagataac catatcccgg catacctggt ctatcagacg taacctggtg
    23941 tcatcattcg agagggtgta agacttaacc tcagccacat tccgtggata gttgacgtgc
    24001 tgccataccg ctacagctcc acattcatca acatctccgc taaggtaggt ttttgccact
    24061 cggttattac ccgataacgc aatttctacc tcctgtatgc cgaaaaacca tgcggctatc
    24121 gcatgacctg cttcatgtat tgcaagatgt ctgggccagc gacagctgcc gaactcaata
    24181 atgatgggtt gcgtactcat atcttccctc tcagaatggc gtttcatcgt cgaatggtgg
    24241 gcgttggtca aaatcatcag gggtattata taactcccat cccggcgcgg attgtgttgc
    24301 gtggcccctg ctggctccgc ttgctttgac tgattcggcc cgcttcccgc cggggcgtac
    24361 cgttctggcg ctgatcacac tgtcggctac tacctgataa ccttgctgtg taccgccgtc
    24421 cttgcccgtc cactgattga tctgcatcgc tccggcaaca ctgactaaat cgcctttaac
    24481 gtgtcctgcc agtgcgtcgg cctgtttgcc gaatgccacc acgcccagcc agaaagtaac
    24541 ctctccgccc tctgctgcat ggcagggcag tgccaccgct aaccggccca ttgccatgct
    24601 tgtaccgttt gctgtggtac gggtctgggg atctgccacc agccgcccgt atgctgaaat
    24661 ttgtgctgtc atagatattt cctcatgtag tcactacctc cggggataat tttcatttct
    24721 tcccttaaag gccactgctc cgccattgct gcggtcgagc gattcccgtc atcacgtccg
    24781 gcctgtgctg ccgtccagtg tgttgcatat tctgtaaatc caatttctgc tggctgcctc
    24841 atccagtggg cgatcatcct caaggtcatt tgcaggcttt gggtgtcgcc ctgtaatgcg
    24901 cgtttgtcag taaacccctt caatgcttca cgctcctgat aggtcatttt ctggctggtt
    24961 tctgtattca ttgcccacct tcgttataag ctaccccccc atagaatctt tttgggggtg
    25021 taatatccct accaatccct actaactgtc tcaggccacg aatggcaagg ctttcagact
    25081 ggtaggcatt caattttgtt gccctacata accctactta tccctactaa atcacttcaa
    25141 cggcatttta ctgcctgttt tatttggtag ggatatgtag ggataagtag ggataaaaat
    25201 atatatccct acctagttta aagccttgct acgtctggcc tcgtctcgtt tttagtaggg
    25261 cagtagggat aagtgccgcc gtaaaagttt caatcatcac tctgcccgct ctccacaccg
    25321 taagcgcgtg gcatgaactc ctcagccttc tcgttatagc caacgttggt ttgcgctctg
    25381 ccgttaatgc ttctcgtcag gtagctggct cggtactctt ttgctgctga ttttatggcg
    25441 cgtgaaaact tattgacgga aagcggttta cccaaaccgt gatactccat aaacgccaga
    25501 taaagatgat aaagatacgt cctcggctct ggctggcctg cccatgtacc tccgcccatc
    25561 atcatgcctt tgggttcgtt catgaaaaaa agcgccgcac acatatcgac aaccggatct
    25621 gtgccgcgtt taacctctag cgcctcctgc gaatcgcgct gctctagtaa tagtgccttt
    25681 gctttgttct ggtcggcaaa ggtggttagc aggtggcgga taatcaccgg gatttctcgc
    25741 ctgatttttg cggttaaatc cgggtctttg tctgcgtctg ataccgggtt attaaacggg
    25801 aatatcacgc ggcggcgggc aatgcccccg tttcgctccg tgaatgtcat cggctcgtta
    25861 ttggttgcca taacaacggc atttatcacg gttgtgaact gcttttcata tttcccatca
    25921 atctctaccg gatcgccgcc cgtaatggct ttaatccccg caccctctcc aacgtagcgg
    25981 gtctggtcag gcataataat caggcttttc cctacgaact gcgcccgtcc ccgtgcggtg
    26041 tccagcgtca tcatgctacc gctggcggtg ttgtgttcac ctgccagcgt ggttgctatg
    26101 ctgctgaata cggatttccc actgccgccc tcgccagtta tctcaatgaa tagctgccag
    26161 tcgtgacgct tcgccagcac cataaacaag gctgctttga tacgctcggc cttatgatgg
    26221 tcgccagccg ttgcatgatt cagccaacgg gtaaaattgg gggcgctggt ggtaatattt
    26281 tcccccggcg ctggcggggt gtaggtgatc ccattgtggt tcatcagcca gttacccggc
    26341 gaatgggcgg aaaacgtctg cgtagcgagg tcataaacgc cgttatcaaa accaatcaga
    26401 tccccgctcc gctcaccaat taccggtatt tgcagcttca tggttgcgac aaccgattta
    26461 atcccctttt cggtgtagtg ggcttcgtgc tcgtcgaaaa ttgccaccat aaccctttct
    26521 aattcgctgt ctggtagctt cacccatacg ccagattcgt agcagtaaac agtgccacta
    26581 tcagggttaa ccgccaacat atcccatcga gatgaaagca accgcgcttt ctggctggct
    26641 gccatttgtg caatatctcc gacttcggct ttacggcggg atttaccgcc cgcaatggcc
    26701 tgtagttgtg gtttcacggc ttccccctga atctggtaca tcgaatcgtt aaacgcagcc
    26761 gtagcgactt ccagcccgtt ttgctggcgg tagtcgtccc agtcggcttt gtagtctgtc
    26821 ggcggcagcg ccacccagcc agccacggat aaggcggctt tctctgcggc atctttgccc
    26881 gtgttcgcct gttcgtcctg ctggtggtcg ttatctgcgg caatgataat ttgcgactgc
    26941 gggtgtttcc ggcgcattac tctggcaacg tggatcaggt tcccggcgtc aattgccgcc
    27001 actgtaagcg cgtccgggcg catcaggtga acggacaggg ccgtcgccag cccctcggca
    27061 attaatacgc tctgcggctg ttctggtgcg tttacggcgt gatatgcccc gcgctttgct
    27121 gaacctgtca ggagtcgctt ctctccctgc ggtgtaatgg tttgcgctgc tgccactgcg
    27181 ccggattcgt ccaccagctc cagcaataac gaaccatccg gcataaccgg atagttaaac
    27241 ccggtcagcc cttttgattg caggtaatca gattctccct gtgtggcact ctggcgcatt
    27301 ccggcataca gacgggcaaa cgtagccctt cgctgctctg catcctctgc tgcctgtttc
    27361 tggcgctcct gctcacgctg ctggcggtca gcttccagtt gctctcttct atggctcgct
    27421 gccaccgggt cggtttccgc tgcccggtaa tcaataccca gcacatcagc ggcaagctgt
    27481 gccgcctccg tagtgtcgca gttgttcacc ttcttaatca ggtctaaacc gtcaccagcg
    27541 ccacactgat tgcagataaa gctaccgcgc ccgttgtcgt cgaatctgaa acggtctttt
    27601 ccaccacatg cagggcatgg agaatggcgg cgcgatgaat caggcacgtc gatagacaat
    27661 ccagccagca cataaggcca gcgcccggcg gcggcactgg tcacttcgcg gataaggtcg
    27721 atattacgca tggttgcctc ctgccacggt cgcgcccatg tctttaacca tgctttgcca
    27781 tatctcgcgc ccgacgtcag tcaatccgtc cttagtgacg caacgctcaa gaagctcgac
    27841 ccccacggtt tcccactgcg ggtaggacac tttcatggct gccagtgtgt agctatcgat
    27901 caggctgcga acgcctttta ctccattgac gatttccaca cggatttgct ggcctgccgc
    27961 gtcaaccatg aagtaattgc caccattgat agccgtaatg tggttataca gtgccgtcgc
    28021 atacttattc gccagcgcat tcagtcggaa atttttagta atcatgtcgc cccctcagtg
    28081 cgccagcggc atttcccgat ttaatggtcg gtgcgttgct catgcgctgc cctccgcatc
    28141 aattctggct atatcgacaa tcgtcaggac tttactcagc cagcaataca cgatttcggc
    28201 ctgttcatcg tttagttgag tggctaaatc ggtcataacg tgagccagcc cattgcgggc
    28261 gcgggtcatg cgttctgctg ttcggtctgc cagcgtaaaa tcatccgggt atgattcttt
    28321 gcgagatagc atcgcttcgg cttccagctc tgccgggtgg cggtagatgg tagttatgtt
    28381 catttgcccg ccctcccttt ggcttctttt tcactgattg agcgcaagaa tgcggcgttt
    28441 gcagcgctat cgttcagcgc ccgcgcaact tctccgatat gtcccagcat tgcgcctaat
    28501 ctgtacatat cagccttagc gctttcctcc gggtagccat cgctaccagc cgcccagaac
    28561 atgaggctac caatggcact tacgcccagc attaaatcat ccacggcatc actggcgcag
    28621 ctctcaattc ctttcagatc atcaggtgta caaccatcaa atttgcagga cactaaatcg
    28681 ttatacatgc tcatgctgcc acctcacgaa tgcgggtaat ctggatgtgg gaaaggcgtt
    28741 catttctggc ctgatctagc gcctcagctt tggcgctgat cgcgctgcgg gcgtcaacga
    28801 tataaacgaa ctcgactaat tctccgctcc gggcggtggc gcatcctgca acacggaagt
    28861 aattaagcat gtgcgccccc tgtgcgaata cgtccggcaa aaaagcaaac gtgatcccgc
    28921 gccagaatgc ggcgggcttc ctgctcagtc tctgcggcga tatggtgaat tttggcggta
    28981 attgtcggca tatcgcggcg aactgcggcg ataatccaga taaattgcgg tttttgggta
    29041 ggggtagtag ccagcatagc ggcagcctct gattcatggt tttgagaacc accaccggaa
    29101 acgccaattt cgctggtggt gaactgtaca gggttggcgt aaccgggaat cagagaaacc
    29161 ggcgcggatt gctccgcccc catacagtcc accattgaac aggtgtaact gcgcggcaca
    29221 aaaaaacacg catggcgcgt gttgtgcgct ctgataatta ccgggacgcc aatcccgaca
    29281 ccagattttg ctggtgcgct ataaccataa accgggttgg cgatagtcgg caagctcttt
    29341 tttatggact ccgaaaactc accttctcgc gtaacgaccc ctagtaaatc ttccagcgca
    29401 cgcgtatttg tcgagaaagt cgatgtggaa cggttcacaa cggtagcaat ccagttcata
    29461 ccaccagcca gcagggggaa tttattcccc acaatcagct ttttagaggt gattatttct
    29521 tggtttaaaa tgtcatcatt tgtcgtcatt ccccgtgcgt ggatgtcagg ttttgtgagg
    29581 ttcaggctaa gttttgctaa gtcattaacc ataacaattg ataacaggca gccgttcgcg
    29641 acctttggtt ttataaggtt gtaagggtta acatttgtta acaggctagc cagattaaag
    29701 gcttctttct gggcggcagg ttcattcagt atcacggcgc acctccacga actcattaac
    29761 gaatctctcc aaaggctgaa cacatggcga ctgatagccc tcgcggtaaa aggtcactcg
    29821 gttatgctcg acgcgctcaa cggtcactac agccccgtga gaatccttgt aacggcttcc
    29881 cggctgtggg ttctggttac gcatggttca cccccgcacg tttcgctaac cagcgctgag
    29941 acagtcgtat taattcagct ttgcgctggt cgtagtcctg ccccagctca attagcgtga
    30001 tattggtgct ttccagatag gagaggtgtt caagctgtgc tgcgttcatg ctgtcccgtg
    30061 gttcgccagt aatgccattt gcctgcgccc actgctttgc cgtcatgcca cccagcacga
    30121 tacgggcgat catgttgctc tcgttgctgt agtggcgctg ctgcgtcgtt ttacccagct
    30181 cggcacgggc catattcagc gcatcgcaca ttggcttaaa gttgtttgcg gcactgatac
    30241 gggcttttag ctggcgacga tagcgggcgg cgatttcagg cacgctacgc tgtaattctt
    30301 cctcgcactg gatgaaataa cggcggatag cgcggccctg ttcggtgcgt tcgaccatcg
    30361 ccagttcttt cgccattccg acacttaaca gatagtcttt gccgggtctg ccagactgtt
    30421 taatcttgct gtaagccgcg ccattcgggc ttttccccag atttggggaa atagttttat
    30481 ggactgagta atcgtgccca atggtgaagt catattcaga gatgcgatca gtgatccatg
    30541 tagagaagtc ttttcccacg cccaacgctt tgtgtaacgc ttttgcgcta acaatattgg
    30601 tttcacgccc accgatatga ccaggaataa ccgggacaat tgcggcaaat tcattaccgt
    30661 taattgtgcc cagagaggcg ttcagatgag gggcagcctc agaaattaat ctgctttttt
    30721 cgatgttcat tttcagtctc cgttaggcgg cgttaaacgc gtccgggtag agtttcagaa
    30781 tgtcggcaat ttcctgctga gaaagtccgt tgtgatcatt tatgttcgca agatggttaa
    30841 catgagtgat cactttcaga acatcaccac ggcaggagaa acggtagcga aggtgtgttc
    30901 cgataccatc aggattcttt tcgtcgatac gctccagagt gattccaagc tggcgttcta
    30961 actctgttgc atagttacga ccagaagaca gacggcagta gcgaagaatg tcattttctg
    31021 tccatccctc aacgccagta cgcagcatat agacgcgggc gcggtgtttc tttggggtac
    31081 gcctaggtac ttgtaccggg ctggtggtcg gcgtaatatc agacgtgcga atatctgagt
    31141 tagccgcctg ctgtacgggg cggtttttct ttttcattac gccacctccc cgcggcgttc
    31201 tgccagccag ttatgaatct ctaccgcgtc aaatgcggtg atcttttcac ctagttttac
    31261 cgggcgagga agtgagccgt cacgtacttt gcggtcgata gtagatacgc taacgcccag
    31321 caattcagct aagcgaaagc gtcgtatata acctttggtg gggatggtct ctatgttcat
    31381 gcttctcgta acctcttttt gattcttgtt gagttgttac gagatacgat aggcacaact
    31441 gattgtaatg tatcggagtt atcagaaaac tccccggaat tatcataatt ccgttatcga
    31501 ctgattcctc ctctccgcaa ccattcaatc agcggcttat catcatccgg gaatggtagt
    31561 ccattaactt tattgttggc tttagctctc agctgtgaaa cgctgccgac tagatcatca
    31621 tcggttaagc caatgccttt aagtaaggcg acaataagct ctgcctgttt tgctgttgtt
    31681 ctgactggtt tctctatcat tgtttcaggt gattgaggtt ggctaacccc tccatttatg
    31741 agatttggta tatccctccc aatagagttg tatacttttt caatctgaca cctggtaaca
    31801 aaaatatcgt ttgccgtgat ggttgcaatc ggtttgactt ctgcttcatt tagcaagcca
    31861 gatgagtaaa ggtgttctgt taatggatga gccataacca cgtagtcatc actgtcaaaa
    31921 tcatcagcgc ttaatggaac atctgcttct ttaaatgaga ggttgaaagc tgtgaatgag
    31981 atttcttcat tattttttaa ggcgccgaaa aatgaagaag gcaggtaacc tattgataat
    32041 gcccaaagtc cggaaagata taaaattggt ttccttaacc caggaatgtc tttgttttga
    32101 taaaaccgct taactcgaac ggttgatgct ggattcatat caaattcagc tttcggcata
    32161 aacaggctta aaggtgattt gtttgtgtat tttgtcatca gtttaggagg gaacttttcc
    32221 tcccattcat ttgattcttc ccaagggcca agtgtaaata gcgccgcttc aaaatcatga
    32281 agtgataaac aaatttcaat agcgccaatt tgaataaaat gaattaaatc atttagatgg
    32341 caattgagca gatctgcggc tcttggcaga gtgcaaaatt ctaatgcagg aatattgtta
    32401 cgtttccata catttacatc attttggcta tcgtttttcc ttgtcatcgc gtcacctttc
    32461 acgctgtgcc ttgtatagtg accggaacag gcggtaaggc ttcccgcttt tcaccccgtc
    32521 gggctagttc cggtctgttc gttaatttgg ttgctaaccg tggttactgg ctggttactt
    32581 tgcctttctc agcggtgtaa cgttgtattc ttcgccgcgc tccagtgcca ccagcaaatc
    32641 acaccactgc gccagcgccg ctttacgctc atcaaaatac tggtgacggt tatagatacc
    32701 ctccacgcct ttaattttgt ggttgaggca tctttcagcc actacgggat caacgcccag
    32761 cgcggctaaa tgggtgcggg ctgtgcgtcg gaagtcgtga atcgtgaaat taggcacatc
    32821 cggcatttca gcgcgaacct tcgccagcgc aacgggcagg gtgctttcct gaatatgggg
    32881 gatcatgcgg ttttgcatct ttctggctgg cagtacccat gcgctgttgc atgagaacgt
    32941 tttaagctct ctcagccatt cgacggctgg cggtgctaat gggatgtcta tagcgtcgcc
    33001 gtttttgctg cgttcctctg gcaagcgcca gacggcttta tcaagatcga actcctccca
    33061 gcgggcggcg cataactcca ttttgcggac gcataacgct aacagtaatt tgaacgtgat
    33121 ttcgttctgg cgactgaatc ccttagccgt gcgcatggct ttgaatagct ggattaactc
    33181 atcgcgggtt aaccagcggt cacgggcaac ttctttccct ccggcgtctg ccacctcaaa
    33241 ggctgaacac gggttaatct ctagcgcgtg ccgtttgatt ccgtagtcga atatgcggcg
    33301 cgtccatctc agcacgtccg ttgctatggt cggcgctcca cggtcaacaa tacctttcag
    33361 catgtcatcg atatggcgcg gtttcacgtc ctctaccttc atgctgccaa tgcaggggtt
    33421 tatatctttg tcgatacggc ggcggagtat atcggggtgc ttccagcgcg ggaggatctg
    33481 acgctcaaag tattcagcgg caagctctga aacgcgcata gcgttcttct ctgcttccat
    33541 cttcgccagt gcttcggttt tgcgcttctg cttctctcct gcaacgtcat agcccagcgc
    33601 aacgcgagcc gatagctctt tggctgtctc tctggccttt gatagtgata gctcgctata
    33661 cgaaccaatt agcatggcgc gggacttccc tgcgagttta tagcggaatc tccatgtggg
    33721 ggttttgtcg gcttcacggt aacgtaggta taaaccgtta ccgtctgccc gcccctcgaa
    33781 tcgctctccg ctcttaatcc atgcgcggat ctgcatgtct gttagttttg gcattttgcg
    33841 aatatccaaa ctttgaatag ggggtgtacc caaagcaatg tacccaaacc tcattgggta
    33901 cacctttgtg tacaccataa tcatgcgctt tattgcgtct ttatgatagc ccatgataac
    33961 aaaaaagccc gctgggttgc gggcttggtg tgatttatgc gtcgtcctaa atgtagctga
    34021 tagcttactc gatattctgg atctgctcgc gcatctgctc aatcaacact ttcagctcga
    34081 tggcggagtt tgtcacttcg gcattgatag atttcgacgc aagagtgttc gactcgcggt
    34141 tgaattcctg catcataaag tccagacgac gaccaacggc ttcttttttc ttcagaatgt
    34201 tgtaggtctc tttgacatgc gcttccaggc ggtccagttc ttcggcaacg tcaattcgtt
    34261 gtgccagcag aaccaactcc tgctccagac ggttgttttc caactgaacc tgagcatctt
    34321 ccagcttcgc gaccagacgc tcacgctgcc attgcaggat ttccggcata tgggcgcgga
    34381 ctttgaccac ttcggcggtg acgccttcca gacgctgctc gatcaatgct ttcagtgcct
    34441 gaccttcggt ttcgcgcgcg acaataaagt cgtccagcgt accatccagc gccgcgagaa
    34501 tttcagcggc aatggcgtcg agatcctgct cctgggctgc catcacgccc ggccagcgca
    34561 gaatatcaac cgggttgatt tccccttcgt cactctgcat ttttacccag ttcgcggcag
    34621 ttaccagctg tttagccagt ttttcattga ggatcagctc accttgtgcg ctaacatctg
    34681 gctcatagcg cagggtacat tccactttac cgcgcgtcag gcgagaacga atacgctcgc
    34741 gaacgacagg ttcaaggcta cggaactgct ccggcagacg aaagtaagtt tccagataac
    34801 gctggtttac cgagcgcatt tcccaggttg cgctccccca ttcacccttg atttcacgcc
    34861 gggcgtaggc ggtcatactg cggatcatag acgttcctgt ttataaaagg agaggtggaa
    34921 ggattatagc catcgatgcc ttgtcaggat aggaataacc gccggaagtc cgtataatgc
    34981 gcagccacat ttgtttcaag ccggagattt caatatgcgt ccagcaggcc gtagcaataa
    35041 tcaggtgcgt cccgttaccc tgactcgtaa ctatacaaaa catgcagaag gctcggtgct
    35101 ggtcgaattt ggcgatacca aagtgttgtg taccgcctct attgaagaag gcgtgccgcg
    35161 cttcctgaaa ggtcagggcc agggctggat caccgcagag tacggcatgc tgccacgttc
    35221 tacccacacc cgtaacgctc gtgaagcggc gaaaggtaag cagggtggac gcacaatgga
    35281 aatccagcgt ctgatcgccc gtgctcttcg cgcggcagta gatttgaaag cgctgggtga
    35341 gttcaccatt acgctggact gcgacgtgct tcaggctgat ggtggcacgc gtaccgcgtc
    35401 gattacgggt gcctgcgtgg cgctggcaga tgcgctacag aagctggtgg aaaacggcaa
    35461 gctgaaaacc aacccgatga aagggatggt agccgcagtt tctgtcggta ttgtaaacgg
    35521 tgaagcaatt tgcgatctgg aatacgttga agactcagcc gcagagaccg acatgaacgt
    35581 agtgatgacc gaagacgggc gcatcattga agtgcagggg acggcagaag gcgagccgtt
    35641 cacccatgaa gagctactca ccttgttggc gctggcccga gggggaatcg aatccattgt
    35701 agcgacgcag aaggcggcgc tggcaaactg atttttaagg cgactgatga gtcgcctttt
    35761 ttttgtctgt agaaaagtaa gatgaggagc gaaggcatga aaccatatca gcgccagttt
    35821 attgaatttg cgcttagcaa gcaggtgtta aagtttggcg agtttacgct gaaatccggg
    35881 cgcaaaagcc cctatttctt caacgccggg ctgtttaata ccgggcgcga cctggcactg
    35941 ttaggccgtt tttacgctga agcattggtg gattctggca ttgagttcga tctgctgttt
    36001 ggccctgctt acaaagggat cccgattgcg accactaccg ccgtggcgct ggcggagcat
    36061 catgaccttg acctgccgta ctgctttaac cgcaaagagg caaaagacca cggtgaaggc
    36121 ggcaatctgg ttggtagcgc gttacaagga cgcgtaatgc tggtagatga tgtgatcacc
    36181 gccggaacgg cgattcgcga atcgatggag attattcagg ctaatggcgc gacgcttgct
    36241 ggcgtattga tttcgcttga tcgtcaggaa cgggggcgcg gcgagatttc ggcgattcag
    36301 gaagttgagc gtgattacaa ctgcaaagtg atctctatca tcaccctgaa agatctgatt
    36361 gcctatctgg aagagaagcc ggaaatggcg gaacatctgg cggcggttaa ggcctatcgc
    36421 gaagagtttg gcgtttaaag aaactcgccg gatgaaaagt catccggcgt catattactg
    36481 caactgcgcg gcaattagcg gccagcgggc gtcaaaatca tccgtcgggc ggtatttaaa
    36541 ttcgctgcgg acaaaacgtg acagcatacc ttcacagaag gccaggatct ggcttgccag
    36601 cagggtttca tcggtggtgt aaccttcacc ctcacgcatt ctcttttcac gcaatacctg
    36661 gcgcagctgc gcttcaatac gctcgaacag ctggttgatg cgcccttgca ggcgatcctg
    36721 ttcaaacatt agcgcatgac cagtgaggat gcgggtcagg ccaggattac gctcaccaaa
    36781 accgagaagc agcaacacaa tcagacgcag gcgcgctgtg gtgtctttct catctttcag
    36841 aatcagattg atgcgagtaa tcaggctatc ttcgataaac tcaatcaggc tatcgaacat
    36901 gcgggtctta ctggggaagt ggcgatacag tgccgcttcg gaaacgccga cagaggcggc
    36961 cagttttgcc gtcgtgatac gttggcttcc atcgctggat tccagcatca gcgccagaga
    37021 ctgaagtatt tcctcgcgac ggttcctttt cgcagtttgt ttttctgcca tgctacaaaa
    37081 tacccctgaa aataagcact tgccaggcgg cacccacgct atgaccgcaa acgaaatgtt
    37141 tgcggctatg ttatgacgtt attcggatgc gtatgtgtta ctgacgacca gagtgaccaa
    37201 agccgccttc accgcggtcg gtggcgtcga aatcttccac cagattaaat tcagcctgta
    37261 ctaccggaac aaaaatcatc tgggcgatgc gttcgccagg ttgaatggtg aagctgtcct
    37321 gaccacggtt ccacacggaa atcatcaact ggccctgata gtcggaatcg atcaatccta
    37381 ccaggttacc aagcacgata ccgtgcttat gtcccaatcc ggagcgcggc agcatcattg
    37441 ccgccagtga aggatcggca atatgaatcg ccagcccggt cggaaccagc gtagtgtcac
    37501 ccggagccag ttctacggcg tcgtcgagac aggcacgcag gtcaagtccg gcagagccag
    37561 aggtggcata agtcgggagc ggaaattcct tcccaacgcg cgggtccaga atcttaacgt
    37621 cgattttttt catcataacg ggtcacgatc tcgtcgagta ataattggcc aaggagctct
    37681 ttgcgctcaa gcggtaagac tttatctccg tcctgccaga aaaggtgtaa tgcgttgttg
    37741 tcgctgttaa atccttgagt tggctgggaa acatcgttcg cgcagatcag atcaaggttt
    37801 ttacggatac gtttttgccg ggcgtattct tccacattat ttgtttcggc ggcaaatcca
    37861 acgacgtagg gtcgatggtc ttttagtgcg gcaacgcctg cgacgatatc ggggttttta
    37921 accattttta ttgttaattc atcaccctgc gtggcctgct ttttgatttt ctctggggcc
    37981 acggtagctg cgcgataatc cgccacggcg gcgcagccga taaaaatatt ttgctgctgt
    38041 acagaagcat tcacggcggc ttccatttcc agcgcggtca tcacatcaac acgtttaaca
    38101 aacggtggcg tcggtagtga aaccggacct gataccagcg tgacgttcgc gccacgacgg
    38161 gcagcggcgg cggcgatagc aaaacccatc ttgccggagc tgtgattaga gatataacgc
    38221 acgggatcga gcggttcacg cgtcgggccg gcggtaatca taatgttcag atgtttcagg
    38281 tcgttgacgg gcgaaaaatg cgctaccgcc atatccacaa tggttaacgg atcgagcatt
    38341 cgcccaggac cgatatcacc acaagcctga ctgccactgt ctggccccca gatgagcaaa
    38401 ccacgggaag caagcacctc taaattatgc tgcgtggcag cggcacggta catctgctgg
    38461 ttcatggcgg ggagcacggc tacaggcgca ggtgtagcca gacaaatcgt cgataccagg
    38521 tcattcgcca ttccggcagc aacacgggca atcaaatctg ccgtggcagg ggcgagaatc
    38581 actaaatcag cccatttacc cagctcaata tggcccatag cggcttcggc tgccgggtcc
    38641 agcagactgt cggaaacggg ataaccagaa accgcctgca agctaagtgg ggtgataaag
    38701 gcttttgccg cttcggtcat ggctacgcgg acgtcggccc cgcgatcgcg caaacgacgc
    38761 accagttcag gggttttata ggcagcaata ccgccgctaa cgccgagaac gattttttta
    38821 ccggccaggc tcatgatgat ttttcctgtt gggtgacacc agaagttggc gattttatca
    38881 caatactttt gttgtcgtgc cttcacctga gaatcacttt gcgaggcgct ttccaggatt
    38941 gaaaactggc cgtcgattta acggaacggc tatgacagga tgcgagcacc acaaaggagg
    39001 tgaaggtgaa aaacaattca cagctgttga tgccgcgcga aaaaatgctg aagtttggta
    39061 ttagcgcctt aacggatgtc gagctgctgg cgctatttct gcgtaccgga acgcgcggta
    39121 aagatgtatt aaccctggca aaagagatgc tggagaattt cggctctctt tatggcttgt
    39181 taacctctga atatgaacaa tttagtggcg ttcatggcat tggcgtggcg aaatttgccc
    39241 agttaaaggg gattgctgaa ctggcgcggc gttactacaa cgtgcggatg cgtgaagaaa
    39301 gccctttact cagcccggag atgacgaggg aatttttaca aagccagctc acgggtgagg
    39361 agcgggagat ctttatggtg atctttctcg actcacaaca ccgggttata acgcatagcc
    39421 gtcttttttc cggcacgcta aaccatgttg aagtccatcc tcgggaaatt atccgcgaag
    39481 cgataaaaat aaacgcctcg gcgctgatct ttgcacataa tcacccttcg ggttgtgctg
    39541 aacccagtaa agcggataaa ctcattactg aacggataat aaagagttgt cagttcatgg
    39601 atttacgcgt gctcgaccat atcgtgattg ggcgtggaga gtatgtttct tttgccgaac
    39661 gcggctggat ttaacccgct atgcgcgatc cttcgggatc tttgtctgtt cgggacttga
    39721 gcacatcgct gagtcagcgt atactacgcc acctttgaga atctcgggtt tggcatttgg
    39781 gcctggcaat cgagagttca catagaactg cgatgaccgg gctgtaaagc ctgacgaggc
    39841 gccaataccc catacgaagc tcgagctaat ttgatttttg gagaatagac atgtcccgag
    39901 tctgccaagt tactggcaag cgtccggtga ccggtaacaa ccgttcccac gcactgaacg
    39961 cgactaaacg ccgtttcctg ccgaacctgc actctcaccg tttctgggtt gagagcgaga
    40021 agcgttttgt caccctgcgc gtatctgcta aaggtatgcg tgtaatcgat aaaaaaggca
    40081 tcgatacagt tctggctgaa ctgcgtgccc gtggcgaaaa gtactaagta cttagaggaa
    40141 ataaatcatg gctaaaggta ttcgtgagaa aatcaagctg gtttcttctg ctggtactgg
    40201 tcacttctat accactacga agaacaaacg tactaagccg gaaaaactgg aactgaaaaa
    40261 attcgatcca gttgttcgcc agcacgtgat ctacaaagaa gcgaaaatca aataattctc
    40321 gctttgatgt aacaaaaaac ctcgctccgg cggggttttt tgttatctgc ttgcccccat
    40381 attgactgca tctgttcatt cctggagatg ctatgcctga attacccgaa gttgaaacca
    40441 gccgccgcgg catagaaccg catctcgttg gtgcaaccat tcttcatgca gtggtgcgca
    40501 acggacgctt gcgctggccg gtttcagaag agatctaccg tttaagcgac caaccagtgc
    40561 ttagcgtgca gcggcgggct aaatatctgc tgctggagct gcctgagggc tggattatca
    40621 ttcatttagg gatgtctggc agcctgcgca tccttccaga agaacttccc cctgaaaagc
    40681 atgaccatgt ggatttggtg atgagcaacg gcaaagtgct gcgctacacc gatccgcgcc
    40741 gctttggtgc ctggctgtgg accaaagagc tggaagggca taatgtgctg gcccatcttg
    40801 gaccggagcc gcttagcgac gatttcaatg gtgagtatct gcatcagaag tgcgcgaaga
    40861 aaaaaacggc gattaaaccg tggctgatgg ataacaagct ggtggtaggg gtagggaata
    40921 tctatgccag cgaatcactg tttgcggcgg ggatccatcc ggatcggctg gcgtcatcac
    40981 tgtcgctggc agagtgtgaa ttgttagctc gggtgattaa agcggtgttg ctgcgttcga
    41041 ttgagcaggg tggtacaacg ctgaaagatt ttctgcaaag tgatggtaaa ccgggctatt
    41101 tcgctcagga attgcaggtt tacgggcgaa aaggtgagcc gtgtcgggtg tgcggtacgc
    41161 cgattgtggc gactaaacat gcgcagcggg caacgtttta ttgtcggcag tgccagaagt
    41221 aattcatgcg cgccggatcg cataccatcc ggcataaacg ctacgctaac ttcgccatca
    41281 gcgcctgatg gacattctcc ggcaggaaat gggtgacatc gccctgatgg cgcgccacct
    41341 ctttcaccaa cgatgaagag ataaacgacc actctttcga cggcatcaga aacacacttt
    41401 ccagttccgg cattaagtgg cgattcatat gcgccagctg catttcatat tcaaaatccg
    41461 ccaccgcacg caggccacga atcagcaccg tagcgtgttg attacgggcg aagttcgcca
    41521 tcaaatcact aaacccaacc acttccacgt tccccagatg cgcggttgcc tgctgtgcca
    41581 gcgccacacg ctcttccagg gtaaacatcg gttttttact ggggctggcg gcaatcgcca
    41641 gaataacgtg atcgaacatt tgcgtggcgc gcgtcacgat atcgatatga ccattggtaa
    41701 tgggatcgaa agtacccgga taaatcgccc gtttttgcat aacaacctca atgcgttttc
    41761 ggtggcaggt aaggttccag cagttgaagc aggcgctgta gcgcgccctg gttttgatac
    41821 agtacttcaa cggcatgacg gccatagaaa ctacggtaat cggcgtcggt gagtaaagag
    41881 gaaacctctt ttgcaagcga cgtggcatcg gtaacggtaa tcagcccgct tgcctgctcc
    41941 agccgcgcgc aaatgtcttt aaagttaaaa gtatgcggcc ccatcaatac cggaatagcg
    42001 tgtgcggcag cttccagcgg attatgccca ccacgttcaa ccagtgaacc gccaacaaag
    42061 gcgagatcgg caatgccata cagtaacatc aactcgccca tcgtatcgcc aaccacaacc
    42121 tgcgtgctgg tggaggggac ttcccctgaa gagcgtgtga tatagcttag tccagcctgg
    42181 cggacaaggt taatcgcatc cgggaagcgt tctggatgac ggggtaccag gatgagcaat
    42241 aaattcggga attgctgtaa taatgcctga tgtgcggcaa tgaccacgct ctcttcacct
    42301 tcgtgagtac tggtggcaat ccataccggg cggtgtggtg cccactggcg gcgcagcgtc
    42361 acggctttag cagccaactg cggcgttaca gaaatatcga atttcaggct accggtaacg
    42421 gttacctggt tattttttgc gcccagcgcc acaaaacgtg caccatcttc ttcattttgc
    42481 gcagcaatca gcgtaatacg acgcagcaag cggcggacga atttacccag tttggcataa
    42541 cctgcggccg agcgggcaga gagtcgcgcg ttagcgatca ccagcggaat attacgttta
    42601 tgtagcgccg caatcaggtt aggccatagt tcggtttcca taatcaacac cagtttgggg
    42661 tcgactttat tcaggaaacg gttgagcgca tcgggcaggt catacggcag ataaacgtgc
    42721 tgaacatcct tcccgaaagc cgattgtacg cgctccgaac cggttggcgt catggttgta
    42781 acggtaatcg gtaaatcagg ataacgatgg cgcagcgcgc gcaccaacgg gatcgccgcc
    42841 agagtttcac cgacggagac ggagtgcagc ataatgccgc ctggttttag cggatggcgg
    42901 taaaaaccgt aacgttcacc ccagcgtttt cgataggccg gagccttacg tccgcgcacc
    42961 cagagccgta tccagatcag cggctgaata aggtagagaa gggcggtgta aagcaattcg
    43021 agcatagtaa atagctgact tatggatgtg ctggggattt tatgtattta gctgtggctt
    43081 taccattact tttcccgttt ttgacttaaa tagcaattta cccgccaatt cttgctgaac
    43141 atctatactt tttttgtatt tttatgcgat tcattgattt ctgctgtacg tgcaggaagg
    43201 aatcgcagag caacagtgaa agaatatggt tgctaatgat tttatttgat tgatttcttt
    43261 attgaaaatc aacaatgtgc ttaaaaatca ttcgggtcgg attgccgcga aagaagtgat
    43321 acactagcgc gtcaatatca gtgcggtatc agtattcagg tagctgttga gcctggggcg
    43381 gtagcgtgct ttttctgctt caccttaatc ggataatctc aacaaaaaga gttacttgtg
    43441 gataagccat ttcgaagaat tttgctcatt aagatgcgtt ttcatgggga tatgttatta
    43501 actactcccg tcattagttc gctgaaaaaa aattaccctg acgcaaaaat cgatgtgctg
    43561 ctttatcagg acaccatccc gatcctgtct gaaaatccag agattaacgc gctctacggc
    43621 ataaaaaata aaaaagcaaa agcctcagaa aaaattgcca acttttttca tctcatcaag
    43681 gtattacgtg ccaataagta tgaccttatc gtcaatctca ccgatcaatg gatggttgct
    43741 atactggttc gcttattaaa tgcccgtgtg aaaatttccc aggattatca tcatcggcag
    43801 tctgcttttt ggcgtaaaag tttcacccat ttggtgccgt tgcagggtgg aaatgtggtg
    43861 gaaagtaact tatccgtgct gaccccattg ggagttgatt cgttggtgaa gcagacaacc
    43921 atgagttacc cgcctgcaag ctggaaacgt atgcgtcgcg aacttgatca cgctggtgtt
    43981 ggacaaaatt atgtggttat ccaacctacg gcgcggcaaa tcttcaaatg ctgggacaac
    44041 gccaagtttt ccgctgtgat tgatgcctta catgctcgtg gttatgaagt cgttctgacg
    44101 tccggcccag ataaagacga tctggcctgc gtcaatgaaa ttgcgcaggg atgccagacg
    44161 ccaccagtaa cggcgctggc tggaaaggtg accttcccgg aacttggtgc gttaatcgat
    44221 catgcgcagc tgtttattgg cgttgattcc gcaccggcgc atattgccgc tgcagttaat
    44281 acgccgctga tatcgctgtt tggtgcgaca gaccatattt tctggcgtcc ctggtcaaat
    44341 aacatgattc aattctgggc gggagattac cgggaaatgc caacgcgcga tcagcgtgac
    44401 cgaaatgaga tgtatctttc ggttattccg gcggcagatg tcattgctgc tgtcgataaa
    44461 ttactgccct cctccacgac aggtacgtcg ttatgatcgt tgctttttgt ttatataaat
    44521 attttccctt tggcggtttg cagcgcgatt ttatgcgtat tgctcagaca gtcgccgccc
    44581 gaggtcatca tgttcgggtt tatgcccagt cgtgggaagg cgaatgccct gatgtatttg
    44641 aactgatcaa agcgccggtt aaatcgcata ccaatcacgg gcgcaatgcg gagtattttg
    44701 cctgggtgca aaaacattta cgtgaacatc ccgtcgatag agtcgttgga ttcaacaaaa
    44761 tgccggggct ggacgtttat tatgccgctg atgtttgtta tgccgagaaa gtagcgcagg
    44821 agaaaggctt tttctatcgc ctgacgtcac gttatcgcca ttatgccgcc tttgagcggg
    44881 caaccttcga acagggtaag ccgacacagt tgctgatgct gacagataag caaatcgccg
    44941 atttccagaa acattatcag actgaagcgg agcgttttca tattctgcca ccggggattt
    45001 atcctgatcg taaatatagc cagcagccag ccaatagccg tgaaatcttc cgtaagaaga
    45061 atggaataac cgaacaacaa tatttattgt tgcaggtcgg ttcagacttc acgcgtaaag
    45121 gtgtcgatcg ttccattgaa gcacttgctt cgttaccgga ttcgctgcgc cacaacacat
    45181 tgctatatgt tgttgggcag gataaaccgc gaaaatttga ggcgctggca gaaaaacgcg
    45241 gcgtgcgcag taatgttcac ttcttctcgg ggcgcaacga tgtctcggaa ttaatggcgg
    45301 cggcggattt attactgcat cctgcctacc aggaagcggc gggaattgtg ctgctggaag
    45361 cgattactgc aggattaccg gtactaacaa ctgccgtttg tggctatgcg cattatattg
    45421 tcgacgctaa ttgcggcgag gctattgctg agccattccg ccaggaaaca ttgaatgaga
    45481 ttttacgcaa agcgttaacg caatcttcat tgcgccaggc ctgggcggaa aatgcgcgac
    45541 attatgctga tacacaagat ttatacagtc tgccagagaa agcggcggat atcataacgg
    45601 gtggtctgga tggttgaact taaagagccg tttgccacgt tatggcgcgg caaagatcct
    45661 tttgaggaag ttaaaacctt gcagggtgag gtatttcgtg aactggaaac tcgccgtact
    45721 ctgcgctttg aaatggcggg caaaagctat tttctcaaat ggcatcgcgg cacgaccctg
    45781 aaagagataa tcaaaaattt actctcattg cggatgccag tattaggcgc tgaccgcgaa
    45841 tggaatgcga ttcatcgact gcgggatgtc ggcgttgata ctatgtatgg ggtggcattt
    45901 ggcgaaaaag gcatgaatcc gctgaccaga acttcattta ttattaccga agatctgaca
    45961 ccaaccataa gtcttgaaga ttactgtgct gactgggcga ctaaccctcc agatgttcgc
    46021 gtaaagcgta tgcttattaa gcgtgtcgcg acgatggtgc gcgatatgca tgctgcgggc
    46081 attaaccacc gtgactgtta tatctgtcat ttcctgctgc acttgccttt ttccggtaag
    46141 gaagaggagt taaaaatttc ggtaattgac ctgcaccggg cgcagcttcg cacgcgcgtt
    46201 ccacgtcgtt ggcgggataa agatcttatt gggctttatt tttcttcgat gaatatcggc
    46261 ctgactcagc gggatatctg gcggtttatg aaagtgtatt ttgccgcccc gcttaaagac
    46321 attctcaagc aggaacaagg actgctgtcg caagcagaag caaaagccac aaaaatcagg
    46381 gaaagaacga ttcgaaaatc gttgtaaccc gtaacttatt tttgccaaaa ttttggatac
    46441 agaataaata tgtctcaact caatgatagt gacatcatcc tttttgagta taattttcat
    46501 tatcaaaata taagatctaa aaatactctt gatatagcat ttggtattga cagaaatttt
    46561 ctttttggat gtggtgtagc catcgcatct attctattaa acaatagaga aatctc
//
LOCUS       DAJSKJ010000011        44695 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000011 DAJSKJ010000000
VERSION     DAJSKJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.011"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(217..759)
                     /locus_tag="ON345_000537"
     CDS             complement(217..759)
                     /locus_tag="ON345_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3070648.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(782..1807)
                     /gene="nagZ"
                     /locus_tag="ON345_000538"
     CDS             complement(782..1807)
                     /gene="nagZ"
                     /locus_tag="ON345_000538"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR3070649.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1818..2642)
                     /gene="thiK"
                     /locus_tag="ON345_000539"
     CDS             complement(1818..2642)
                     /gene="thiK"
                     /locus_tag="ON345_000539"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR3070650.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWWQLLIKQ"
     gene            complement(2623..3264)
                     /gene="lpoB"
                     /locus_tag="ON345_000540"
     CDS             complement(2623..3264)
                     /gene="lpoB"
                     /locus_tag="ON345_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR3070651.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3278..3655)
                     /locus_tag="ON345_000541"
     CDS             complement(3278..3655)
                     /locus_tag="ON345_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR3070652.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3658..4017)
                     /gene="hinT"
                     /locus_tag="ON345_000542"
     CDS             complement(3658..4017)
                     /gene="hinT"
                     /locus_tag="ON345_000542"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR3070653.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4351..6540
                     /gene="fhuE"
                     /locus_tag="ON345_000543"
     CDS             4351..6540
                     /gene="fhuE"
                     /locus_tag="ON345_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCR3070654.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTNTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(6600..8033)
                     /gene="ptsG"
                     /locus_tag="ON345_000544"
     CDS             complement(6600..8033)
                     /gene="ptsG"
                     /locus_tag="ON345_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR3070655.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8328..9125)
                     /locus_tag="ON345_000545"
     CDS             complement(8328..9125)
                     /locus_tag="ON345_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3070656.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9136..10140)
                     /gene="holB"
                     /locus_tag="ON345_000546"
     CDS             complement(9136..10140)
                     /gene="holB"
                     /locus_tag="ON345_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR3070657.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10137..10778)
                     /locus_tag="ON345_000547"
     CDS             complement(10137..10778)
                     /locus_tag="ON345_000547"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR3070658.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10768..11790)
                     /gene="yceG"
                     /locus_tag="ON345_000548"
     CDS             complement(10768..11790)
                     /gene="yceG"
                     /locus_tag="ON345_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR3070659.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREILKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSVWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGMPPGAIATPGANSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11793..12602)
                     /gene="pabC"
                     /locus_tag="ON345_000549"
     CDS             complement(11793..12602)
                     /gene="pabC"
                     /locus_tag="ON345_000549"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR3070660.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12722..13963)
                     /gene="fabF"
                     /locus_tag="ON345_000550"
     CDS             complement(12722..13963)
                     /gene="fabF"
                     /locus_tag="ON345_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR3070661.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14051..14287)
                     /gene="acpP"
                     /locus_tag="ON345_000551"
     CDS             complement(14051..14287)
                     /gene="acpP"
                     /locus_tag="ON345_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3070662.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14498..15232)
                     /gene="fabG"
                     /locus_tag="ON345_000552"
     CDS             complement(14498..15232)
                     /gene="fabG"
                     /locus_tag="ON345_000552"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR3070663.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15245..16174)
                     /gene="fabD"
                     /locus_tag="ON345_000553"
     CDS             complement(15245..16174)
                     /gene="fabD"
                     /locus_tag="ON345_000553"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3070664.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAGSYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16190..17143)
                     /gene="fabH"
                     /locus_tag="ON345_000554"
     CDS             complement(16190..17143)
                     /gene="fabH"
                     /locus_tag="ON345_000554"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR3070665.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17211..18281)
                     /gene="plsX"
                     /locus_tag="ON345_000555"
     CDS             complement(17211..18281)
                     /gene="plsX"
                     /locus_tag="ON345_000555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR3070666.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18362..18535)
                     /gene="rpmF"
                     /locus_tag="ON345_000556"
     CDS             complement(18362..18535)
                     /gene="rpmF"
                     /locus_tag="ON345_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3070667.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18587..19108)
                     /gene="yceD"
                     /locus_tag="ON345_000557"
     CDS             complement(18587..19108)
                     /gene="yceD"
                     /locus_tag="ON345_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR3070668.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            19307..19891
                     /gene="yceF"
                     /locus_tag="ON345_000558"
     CDS             19307..19891
                     /gene="yceF"
                     /locus_tag="ON345_000558"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR3070669.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20003..20962)
                     /gene="rluC"
                     /locus_tag="ON345_000559"
     CDS             complement(20003..20962)
                     /gene="rluC"
                     /locus_tag="ON345_000559"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR3070670.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21535..24720
                     /gene="rne"
                     /locus_tag="ON345_000560"
     CDS             21535..24720
                     /gene="rne"
                     /locus_tag="ON345_000560"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR3070671.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24917..25870)
                     /gene="flgL"
                     /locus_tag="ON345_000561"
     CDS             complement(24917..25870)
                     /gene="flgL"
                     /locus_tag="ON345_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR3070672.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNKVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25882..27525)
                     /gene="flgK"
                     /locus_tag="ON345_000562"
     CDS             complement(25882..27525)
                     /gene="flgK"
                     /locus_tag="ON345_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR3070673.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALALAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRFASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27591..28532)
                     /gene="flgJ"
                     /locus_tag="ON345_000563"
     CDS             complement(27591..28532)
                     /gene="flgJ"
                     /locus_tag="ON345_000563"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR3070674.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28532..29629)
                     /gene="flgI"
                     /locus_tag="ON345_000564"
     CDS             complement(28532..29629)
                     /gene="flgI"
                     /locus_tag="ON345_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR3070675.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGAGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSACLNNVVRA
                     LNALGATPMDLMSILQSIQSAGCLRAKLEII"
     gene            complement(29641..30339)
                     /gene="flgH"
                     /locus_tag="ON345_000565"
     CDS             complement(29641..30339)
                     /gene="flgH"
                     /locus_tag="ON345_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR3070676.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30392..31174)
                     /gene="flgG"
                     /locus_tag="ON345_000566"
     CDS             complement(30392..31174)
                     /gene="flgG"
                     /locus_tag="ON345_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR3070677.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31312..32067)
                     /gene="flgF"
                     /locus_tag="ON345_000567"
     CDS             complement(31312..32067)
                     /gene="flgF"
                     /locus_tag="ON345_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR3070678.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32087..33295)
                     /gene="flgE"
                     /locus_tag="ON345_000568"
     CDS             complement(32087..33295)
                     /gene="flgE"
                     /locus_tag="ON345_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR3070679.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQNFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(33320..34015)
                     /gene="flgD"
                     /locus_tag="ON345_000569"
     CDS             complement(33320..34015)
                     /gene="flgD"
                     /locus_tag="ON345_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR3070680.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34027..34431)
                     /gene="flgC"
                     /locus_tag="ON345_000570"
     CDS             complement(34027..34431)
                     /gene="flgC"
                     /locus_tag="ON345_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR3070681.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNSMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34435..34851)
                     /gene="flgB"
                     /locus_tag="ON345_000571"
     CDS             complement(34435..34851)
                     /gene="flgB"
                     /locus_tag="ON345_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR3070682.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35007..35666
                     /gene="flgA"
                     /locus_tag="ON345_000572"
     CDS             35007..35666
                     /gene="flgA"
                     /locus_tag="ON345_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR3070683.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35742..36035
                     /gene="flgM"
                     /locus_tag="ON345_000573"
     CDS             35742..36035
                     /gene="flgM"
                     /locus_tag="ON345_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR3070684.1"
                     /translation="MSIDHTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36040..36456
                     /gene="flgN"
                     /locus_tag="ON345_000574"
     CDS             36040..36456
                     /gene="flgN"
                     /locus_tag="ON345_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR3070685.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36496..38031)
                     /gene="murJ"
                     /locus_tag="ON345_000575"
     CDS             complement(36496..38031)
                     /gene="murJ"
                     /locus_tag="ON345_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR3070686.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38141..39064)
                     /locus_tag="ON345_000576"
     CDS             complement(38141..39064)
                     /locus_tag="ON345_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3070687.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39066..39713)
                     /locus_tag="ON345_000577"
     CDS             complement(39066..39713)
                     /locus_tag="ON345_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR3070688.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39724..40308)
                     /gene="rimJ"
                     /locus_tag="ON345_000578"
     CDS             complement(39724..40308)
                     /gene="rimJ"
                     /locus_tag="ON345_000578"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3070689.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40544..41752
                     /gene="mdtH"
                     /locus_tag="ON345_000579"
     CDS             40544..41752
                     /gene="mdtH"
                     /locus_tag="ON345_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR3070690.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41816..42463
                     /gene="grxB"
                     /locus_tag="ON345_000580"
     CDS             41816..42463
                     /gene="grxB"
                     /locus_tag="ON345_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR3070691.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42597..43157
                     /locus_tag="ON345_000581"
     CDS             42597..43157
                     /locus_tag="ON345_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3070692.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43263..44309
                     /gene="pyrC"
                     /locus_tag="ON345_000582"
     CDS             43263..44309
                     /gene="pyrC"
                     /locus_tag="ON345_000582"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR3070693.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44383..44628
                     /gene="dinI"
                     /locus_tag="ON345_000583"
     CDS             44383..44628
                     /gene="dinI"
                     /locus_tag="ON345_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR3070694.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10778 a        12098 c        11145 g        10674 t
ORIGIN      
        1 caaaaaatag agtttattgt ttattatttg agcaaattcg gaagagtaat atagggtgaa
       61 gcgtatatcg agggcgtttg gtcgtcctga taagatgcgc agcatcgcat caggcattga
      121 tgctcgactg tcggatgcgg cgtggacgcc ttatccgact tacagaattc ggagcgcatc
      181 aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt tgaacgcttt
      241 aatgcgctgt aaatgcgggg agatattctt gaatttgtgc gtctgctctt cgtcccagac
      301 aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagtg cttcatcatt
      361 acgcgacaaa ataaccagac aacgatcgcg attcttctcg cggaagttgg tcacacactt
      421 agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt tctcataagg
      481 gaacaaatta gggttgaaga tcacctggcg gatgtcgcaa agaaaaccaa tccgttccgc
      541 ccagtatccg cccaggccaa cgccgcaaat tagtggacgc tcgtcaacgt tcagttgcaa
      601 cattttgtcc acttctttaa gcagatgctg catatcatgt ttcggatgcc gcgtactgta
      661 gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc
      721 cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg
      781 gttagtgacc tgctttctct tcctgccagc gttcgtgtaa ctgattcaga cgggtgctga
      841 tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca
      901 aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt
      961 tacgattatt gcagaccagg atcatatcgc aacccgcatc cagtgatgcc tgcccgcgtt
     1021 cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaatcacgc
     1081 cgtcaaaacc cagttcctga cgcaaaacgg ttttcagcca gtagggagaa ccgctcgccg
     1141 gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt
     1201 cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct tgtggacgtg
     1261 gatcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgtcct gggaagtgtt
     1321 tcccggtcgt tttcattccg gcttcatgca taccatcaat aaaccggctg gcaattgcca
     1381 gggctttttg tggatcggca tgataagaac gctcgccaat cgccgcgctg atatgcccga
     1441 catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca
     1501 gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcctgacagc gcagcgaatg
     1561 attgcgccgc tggcaagcgg gtaaaacctt cacgaaaacg ctgcacgcgt ccaccttcct
     1621 gatctaccgc caccaccaga tgattgcgcg aagctgcgcg gatctggcgc accagttcac
     1681 gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc accagcggat
     1741 gcgcaagtat ttcacgctct tccgcgtcca gttcgtaacc tttgacatcc aacattactg
     1801 gacccacact gctctcctta ttgttttatt aatagctgcc accaggtgtc atcggccagc
     1861 ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc tgctttgagc
     1921 atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc
     1981 gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc cacccacacc
     2041 gccgccagtt ccagcgcgat atcaccatct ccggcatact cccagtcgat gagttttaac
     2101 cctgacgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt
     2161 aaaggccgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccactgtc
     2221 cgccgcgccg gatcgctttg ctgccagtac agttccagta acggcaacag cgttattcgc
     2281 cagccaaaac gtggttgttg atgtagataa tacagcaagc ctgccagttc gttggtatcc
     2341 ggcaaatacg tttttaccgc gccgggcaga tagtcgacta ccatccagtc acggagatat
     2401 aaatgcggct tcggtgcaat gcttgcgggt agttgtgata aagcccgata ctggcgcaag
     2461 aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctga
     2521 tgttcaatga gaaaactccc gccactaagc ccactattaa acgtcgtgac gggatgaaac
     2581 tgcgggaaaa agcgcgacag caattcgtcg cgcatgatgg gattattgct gcgaaacggc
     2641 acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt
     2701 cggagcgtta acgttgccag aggcgctgga gtacagcacg taatgagcgc cgacattgcg
     2761 ggcaatgcct atggctttac tacgggtacc taaactgtcc tgcggcgaca aacctaactg
     2821 ttgcttagcc atcgacagct gctgggcgga aaccagggta aatttcccgt tattagccag
     2881 cgcatttctc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt
     2941 aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact
     3001 gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc
     3061 cgtttgatct tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac
     3121 tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac
     3181 tggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa
     3241 ggcgtagcga ctcatttttg tcatcaagat tcacccctta caaatataga taaagtctga
     3301 ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa
     3361 tggtaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt
     3421 aaaaacgata atgaacggta acgggttctt gcctttcgtt ataaagcgtt gaggatgctg
     3481 aaggttgaat atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata
     3541 aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac
     3601 agcccaccag cagtaacaac gccagagaca ccagcccaaa gcatcctttt ctcatcgtta
     3661 cagaccttta tgcgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat
     3721 gtggtaaacc tcttgtccgc catggcggtt ggtattcatg atcagacgat agccatcttc
     3781 ggcaatacct tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg
     3841 ctcatgctca gctgagacgt cgttcacagt cggaatgagg atattcggaa tgatcagaat
     3901 atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac
     3961 gatatcggag gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac
     4021 attttccttt ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta
     4081 tagagtatgg tcgagttaaa cctgctcttt caaccttaac tcacgatttc ttaagcaaaa
     4141 aatcgttttg ctgccgtata tatcgccatt attcccattt ctgcctgcga atgcgctgaa
     4201 aagacattct ctgtttggtt tacacgacaa atatgaatgc gtatatttct catttgcatt
     4261 tacaaacaaa attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc
     4321 ctgacgacaa cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat
     4381 caatatcaag ccatcaccaa accgtcacta cttgccggtt gcatagcact ggcactatta
     4441 ccttctgccg cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc
     4501 acagctccag atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa
     4561 atgcagatga ctcaacgtga tattcctcag tcggtcacta ttgttagcca gcagcggatg
     4621 gaagatcagc agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa
     4681 agtcaggcgg attccgatcg tgctctttat tattcccgcg gattccagat cgataactat
     4741 atggttgatg gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct
     4801 gatatggcac tctttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg
     4861 acgggtaatc catctgcggc aattaatatg gttcgaaaac acgcgaccag tcgtgaattt
     4921 aaaggcgatg tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta
     4981 caaagcccac tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat
     5041 aacgactcat ggctggaccg ctacaacagt gaaaagacct tcttctcggg cattgtcgat
     5101 gctgatttag gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt
     5161 aatagcccta cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac
     5221 gatcgtgcac gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc
     5281 tttatgaccc tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac
     5341 tctgaagtcg aatttgatag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat
     5401 ggtatgctgg ttgggccata cagtaattat ggacctggct ttgattatgt cggcggcacc
     5461 ggttggaaca gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtatttat
     5521 gaattgtttg gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat
     5581 cgttacttca gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt
     5641 aatggcaatt tcccacaaac cgactggtca ccacagagcc tggcacagga cgataccaca
     5701 catatgaaat cgttatatgc tgccactcga gtcacccttg ccgatccgct gcatctgatc
     5761 ctcggcgcac gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac
     5821 cacaccacgc cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc
     5881 agctatacct ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct
     5941 ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg
     6001 accaccacgt tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca
     6061 cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa
     6121 ggggtggaat ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca
     6181 acgcgctata ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc
     6241 acggttaaaa tgttcaccag ctatcggttg cctgtcatgc cagagttgac ggtcggcggt
     6301 ggtgttaact ggcaaaatcg cgtgtatacc aacaccgtga caccgtatgg caccttccgc
     6361 gccgagcaag gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac
     6421 ttctcgttac aggggaacgt caataacctg ttcgacaaaa cctacgatac caacgtggaa
     6481 ggttctatcg tctacggcac accgcgtaat ttcagcatta ccggcacgta tcaattctga
     6541 ataacacctg taaaaaaggc agccatctgg ctgccttagt ctccccaacg tcttacggat
     6601 tagtggttac ggatgtactc atccatctcg gttttcaggt tatcggattt agtaccgaaa
     6661 atcgcctgta caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca
     6721 gcctgatcca ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg
     6781 aggttagtaa tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg
     6841 gtacctgtcg cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt
     6901 gctttaatca gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc
     6961 gggaacagcc acagtttgct gctgttacca gacagaacga taaagtcgat cagaccgtgc
     7021 gagaacgacg taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc
     7081 agaatcgcgt ggatgatgta caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc
     7141 tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc
     7201 gcgcggtttt ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac
     7261 attttgaaca ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc
     7321 ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg
     7381 aaaggtacgt tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg
     7441 taaatgccaa acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt
     7501 gcagaaccaa tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg
     7561 ccagaaatga tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta
     7621 atacggtaga aacggttaaa catgtacgct gcgatcgcac cggagataat ccctccgagt
     7681 acgccagtat ccgccaggtg tttagaggcg atttcttcag caggtaaatg cagtaccagt
     7741 ggcgcaacca cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg
     7801 gatacgccat cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg
     7861 tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag
     7921 ctgaaattcg cggaaccgac gcccagcaga atacctgcga taggtagtac ggataccggc
     7981 agcatcagcg atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag
     8041 agtgctcctg agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct
     8101 cgccgtgtac agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg
     8161 ccaatttgtt tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt
     8221 gacaaaacat atgtcacaat atttcaaacg cggttattca ccgcccaatt ttcggcggtg
     8281 aactttactc gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat
     8341 tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc
     8401 agttcttcaa cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc
     8461 gcaggttgat tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc
     8521 accagtaatc gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg
     8581 aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt
     8641 tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc
     8701 agaattgcca gcgtatcggc acgggcgtca cgggtatgga cgataaccgg cttgttcagt
     8761 tcacgaccaa tctggatatg atggatgaag gactcttgct gacgtacttt agtttccggt
     8821 gtgtaataat aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga
     8881 cggcgtaaat cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa
     8941 aatacgacgt tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt
     9001 gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc
     9061 acgtccttat gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa
     9121 aacatgatgt ctctcttaaa gatgaggaac cggtagcaca acgcccggtt gcaggtaatg
     9181 ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg taacagacat
     9241 taactgttca cgaatgtggc aaacatcccc cagtatagcc tgcaggcgcg agggagaaag
     9301 atggtttgcc agttcggcga ccaggcccgg cacatcaaca ttggtcacct gcgcagcacc
     9361 atgatggcgt tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc
     9421 ttgttcatga ttaagggccg ccagcagcga ataccagtcg cccgatggca cgctatatgc
     9481 caacgcctga cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc
     9541 cgcgccaggc gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt
     9601 cacttcgcgt gaaagccagg tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa
     9661 acgacaacga ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa
     9721 ccaggtttct gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt
     9781 cagtaaggca gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt
     9841 cagcttttcg gtgacctcac gtaccgcatc aatgcccagc gtatttttcc ctttttcggg
     9901 agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg
     9961 accgcaactt ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat
    10021 taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc
    10081 ggcctgatag ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat
    10141 gcgtccaact ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc
    10201 agcggctggg tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag
    10261 cgggcgcggg tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg
    10321 cgcgcacgcg cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtcg
    10381 gggcgaaaat ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca
    10441 cgtcctccgc cctgatacgc ctgagtggag agatcgtggc gatcgccaat cacccaggtg
    10501 ccgttagcca gagctggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac
    10561 atcagaactt cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc
    10621 aggcttctta acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg
    10681 cggataccca gttgctcaag cgtctcaacc accacattac gcgcggtagt tttgcctgcg
    10741 ccttccagcc cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag
    10801 ataatcctgc acagacttgt tatggctggc aagattggta ttaaacgtgt gaccaccttt
    10861 accatcggcc acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga
    10921 attcgccccc ggtgtagcta tcgcacccgg cggcataccg gtaatggtat aggtgttata
    10981 cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc
    11041 gtaaatcacg gttgggtcgg tctgtaggcg cataccaatg cgtaaacggt tgataaatac
    11101 cgaggcaacc tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat
    11161 cgtcaccagc tgatttttat ctttataagg cagaccgtcc gcacgccctt cccagacgct
    11221 atcgaccgct ttcaccattt tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt
    11281 ggcggtatac atccaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag
    11341 ttcaagtgcc tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg
    11401 agcctcacgc aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg
    11461 gaactgtgct tctttaccgc tttccagcaa tttcagaatc tcgcgcacgg tcatctgcgg
    11521 tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcaacca
    11581 ttgaaaaacc cgtgggcgat tgatgatctt atcggcataa agctgttcac caagcgccag
    11641 acgtccggtc cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc
    11701 ggcaagatgg cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa
    11761 taacaagata atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct
    11821 aaatattcat ataacgttgc tgacgaaaag gagacatcgc cacaggcacg tacgggcatc
    11881 actggcatta acgcattaca aataaccatc tcatctgcct gcaacgcctc ttccagagag
    11941 gcttgcactt cgacaagctg ataagaggat tgtgccagca aacggataca gaattgtcgc
    12001 ataatgccgt taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc
    12061 cagaacaaat tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc
    12121 tcatcagcgt ttgtctgctc aagatgagag cggatcaata cttgctcaag ccgattaaga
    12181 tgtttaatac ctgcaagatg gggattgcgc cccagccgca ccgggcttag cgccaacgtc
    12241 atcccttcgt tacgcaaacg gtcgtaatgt gcaggataaa ccgtaacgga gagaatccgc
    12301 gttgccggtc cactgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg
    12361 actttcagca caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga
    12421 ggccagaagt cacagaaaat catcaaccgc tgacaagcat cctgtagtcg ctggatatgc
    12481 gccgataaca aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca
    12541 aactgcgttg cccgatcgct tactgccagc gattcctgct tatgaccgtt aattaagaac
    12601 ataccggctc cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg
    12661 gaataaaagc taagaaaaaa ggcccgcaag cggacctttt atatgggtgg gaaatgacaa
    12721 cttagatctt tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca
    12781 gcgtgtattc cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac
    12841 cttcatccgg gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga
    12901 tggagtagat agactctact gcccccgccg cacctaacag gtgaccggtc atagatttcg
    12961 tggagcttac caacacacgg cttgcagctt caccgaagat ggctttcacc gcctgcgctt
    13021 cagctttatc gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg
    13081 cttcaatgcc tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt
    13141 ctggcggtga cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt
    13201 aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc tagtaccagc ataccggcac
    13261 catcgcccag tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt
    13321 tatcattgcg ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac
    13381 tggctttctc tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg
    13441 cagcatgacc aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc
    13501 cacgcaggcc atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa
    13561 cgaagaatgg gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga
    13621 tcagtccgag gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt
    13681 ccgttatttc aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa
    13741 tgaaggcatc catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct
    13801 ttactaagcc agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga
    13861 tgccactctg accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag
    13921 acaacatgcc cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccagggag
    13981 ggaaaaaatg attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg
    14041 agatgttcac ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga
    14101 ttttctcagc ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca
    14161 gctcaacggt gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa
    14221 cttcttcctg cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag
    14281 tgctcatact cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata
    14341 aaatgttgaa aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt
    14401 gaggccttac cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca
    14461 gactgcgcgc aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt
    14521 tcacatgcaa agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg
    14581 cgttggcgat ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg
    14641 cacgctggtc atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa
    14701 cgtttacagt aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca
    14761 agcccgcttt cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag
    14821 aaccgatagt gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg
    14881 acagacggaa aacagatgaa aggttggttt cgataatatc gttccactct tcatctttca
    14941 ttcgcattaa caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac
    15001 caaattctgc gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca
    15061 acatcagacc tttgccgtta gcacctaaat aatcactgat cgcctgagcg ccattttcac
    15121 tggtcgcagt gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc
    15181 caattccgcg gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc
    15241 tcttttaaag ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca
    15301 gggtgtcgac aatgcgtttc gtcaggccag ttagcacttt gcccgggccg acttcataga
    15361 gatgttctac gccttgcgct gccatgtact caacagactt cgtccactga accgggttat
    15421 acaactgacg taccagtgcg tcacgaatgg catcaccatt ggtttcgcat ttcacatcaa
    15481 cgttattcac aacaggaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca
    15541 gtttgtcggc tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca
    15601 gcgcgcgttt tgcgcccgca gctttacagg cagcgccagc acgctcaacc gcctctttat
    15661 gaccggcaat aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctt
    15721 ctgcagcttc ttcacacgct ttcgcaatag acgcatcatc cagaccgatg attgccgcca
    15781 tagcgcccgt gccttctggt acggcttctt gcatgaactt gccgcgcatc tcaaccagac
    15841 gcaccgcatc agcgaaatca atcacgccag cgcaaaccag cgcggagtat tcccccaggc
    15901 tgtgaccggc catcattgcc ggtgctttac cgccctgctg ctgccatacg cgatacagcg
    15961 caacagatgc agtcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg
    16021 gcccctgctg ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg
    16081 tttcttcgac aattggatag ctccccgcca tatcagccag cattccaacg gtttgagaac
    16141 cctgtccagg gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa
    16201 ccagcgcgga gccccaggtg aatccaccgc caaaggcttc aagcagaacc aactgccccg
    16261 gcttaatgcg cccgtcgcgt acagcttcat ccagcgcgca cggaacagag gccgcagagg
    16321 tattaccgtg gcgatccagc gtcaccacga cattgtccat agacataccg agttttttcg
    16381 ccgttgcact gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac
    16441 ggtcaagatt atttgccgcc agcgtctcat caacgatgtg cgccagttcc gttaccgcaa
    16501 ccttgaagac ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacacggt
    16561 cagcattagg cagcgtcagc aactcaccat agctaccgtc ggcatgcaga tgggtggaga
    16621 tgattcccgg ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa
    16681 taatagtccc acgatcggtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca
    16741 gagcatactt caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga
    16801 aacctgcgca ggctgctgca acgtcaaatg ccgggcaacc tttaatgccc aacatgcttt
    16861 gaatctgaca agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca
    16921 ggccaatctg gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc
    16981 ccatggttga aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag
    17041 tgacaatcca ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt
    17101 gttcgggcag atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt
    17161 tcggttatat accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga
    17221 gttccgcttt tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga
    17281 gcggcaattc gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc
    17341 gcaaaagctc gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac
    17401 agacaggcgc cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt
    17461 tgtagccaac gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat
    17521 ttcagcagag aaaggaacat cctgacaaca ccttccatcg tctttaacgt gacatttcct
    17581 gtaaagccgt cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga
    17641 tagccgatat aattgataga agggattgtt ttaagcaccg ctgaggcatc ccgaatactg
    17701 tcgagaccct ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt
    17761 tccaccacct cttcagccag aactgagccc ataatggcaa attgcaccaa cattgtgctg
    17821 tcacaatcga cgttggcccc taaatcgagg accaccgttt tgcccttttg ctgatgtggt
    17881 aataccgtca ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc
    17941 agccccatca gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc
    18001 accagctcca gggccatacg cattgaactc ccacgactgg cgcggatagc ttgcgaaggc
    18061 cgggcatcac tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca
    18121 aagtcagctt tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg
    18181 agttgcgaat tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa
    18241 tcccctccca tgacatctaa cgccagggtt agacgtgtca aggtatcgtc gccgcccggt
    18301 ttggtctttc cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg
    18361 attacttagc gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca
    18421 ggtgtttttc accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat
    18481 gggaacgacg catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc
    18541 ttactcctca attacggttt atccccgatc caacggggag caccaattac ttacgcttta
    18601 agctggctaa tacggcaaat gggtttggct tttgcacttc ttcaggcagt tcaccaaaga
    18661 ccatgtccgc ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg
    18721 cgaggatgat ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct
    18781 caatcggttc atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa
    18841 aacaatacgt tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg
    18901 ttaccgtcac cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg
    18961 acatggagca ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct
    19021 gatcaggggt atagataccc tggtaatcaa ggcgtttttg agccgtacga accggatcga
    19081 gagtcagggg taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag
    19141 agtcaaagaa aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca cattgcgggt
    19201 tactgcttta ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata
    19261 gtttaaaatg gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat
    19321 tttagcctcc acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga
    19381 atgtgcagca ccagaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt
    19441 gcttcgactg gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg atcatttaat
    19501 tattggttca gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga
    19561 agaaaatgcc cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg
    19621 actggcgttg tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt
    19681 ccatttccgt cacctgagcg aggcggagat tgataattac gtgcgtaagg agcatcccct
    19741 gcactgcgcg ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga
    19801 gggacgtgac cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg
    19861 tcgggaaggg aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca
    19921 tccggcaatc ggtgcaggcg gcctgatgcg acgtaaccgc cttatcaggc caaccgccca
    19981 ttatcaggct tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca
    20041 aaccttcatc catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact
    20101 tcaacgctgc ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc
    20161 tgtcaaattc acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg
    20221 tatgcacacg gatctgatga gtacgtccgg ttaccgggct acaacgcacc agggtggcaa
    20281 atgcatagcg ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac
    20341 gcacgatacg ttcgccgctt tgcagaatat ttttcaataa cggcgcttga acgctcttca
    20401 catgcgactg ccactgactg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac
    20461 gtaattgctc atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg
    20521 aagtgtcccg gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc
    20581 gcaaaccttc gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag
    20641 gtttattcag caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca
    20701 ccttttgcag atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggga
    20761 tacgcacctc atcacccgct tcgagtttat attcaggttt aatacgtttt ttgttcaccc
    20821 gcacttcgcc tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg
    20881 tacgcaaaaa gttatcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta
    20941 cggatggagt ctctgttttc atggttggcg attctaatta gccaacagga ttcgcgccac
    21001 tcatttttct atgcttatat ttacttttgc accttattac ttcactgcgt gatcacttta
    21061 ttgatggtta ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat
    21121 aaatggtaaa agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg
    21181 tgtccatcct tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat
    21241 ccacgcagca atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc
    21301 agtccttacc ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata
    21361 attgcgctgt ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga
    21421 cgataaccgt gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag
    21481 gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa
    21541 agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag
    21601 cgtctgtatg acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac
    21661 aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct
    21721 gaacgtcacg gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac
    21781 agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt
    21841 cagatcgata aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg
    21901 gcgggtagct atctggttct gatgccgaac aacccgcgcg cgggtggcat ttctcgccgt
    21961 atcgaaggcg acgaccgtac cgaattaaaa gaagcactgg caagccttga actgccggaa
    22021 ggcatggggc ttatcgtgcg caccgctggc gtcggcaaat ctgctgaggc gctgcaatgg
    22081 gatttaagct tccgtctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg
    22141 gccccgttcc tgattcatca ggagagcaac gtaatcgttc gcgcattccg cgattactta
    22201 cgtcaggaca tcggcgaaat ccttatcgat aacccgaaag tgctcgaact ggcacgtcag
    22261 catatcgctg cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag
    22321 atcccgctgt tcagccacta ccagatcgag tcgcagatcg agtccgcctt ccagcgtgaa
    22381 gtccgtctgc catccggtgg ttccattgtt atcgacagca ccgaagcgtt aacggccatc
    22441 gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg aagaaaccgc gtttaacact
    22501 aacctcgaag ctgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg
    22561 attgttatcg acttcatcga catgacgcct gtacgccacc agcgtgcggt agaaaaccgt
    22621 ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt
    22681 ggcctgctgg aaatgtcccg tcagcgcctg agcccatcac tgggtgaatc cagtcatcac
    22741 gtttgtccgc gttgttctgg tactggcacc gtgcgtgaca acgaatcgct gtcgctctct
    22801 attctgcgtc tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt
    22861 gttcctgtgc caatcgcttc ttacctgctg aatgaaaaac gttctgcggt aaatgccatt
    22921 gaaactcgtc aggacggtgt gcgctgtgta attgtgccaa acgatcagat ggaaaccccg
    22981 cactaccacg tgctgcgcgt gcgtaaaggg gaggaaaccc caaccttaag ctacatgctg
    23041 ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag
    23101 cgtccggaac aacctgcgct ggcaaccttt gccatgccgg atgtgccgcc agcgccaacg
    23161 ccagctgaac ctgccgcgac tgtcgtagcc ccagcaccga aagctgcaac ggcaacacca
    23221 gcagctcctg cacaacctgg gctgttaagc cgcttcttcg gcgcactgaa agcgctgttc
    23281 agcggtggtg aagaagccaa accgaccgag caaccaacac cgaaagcaga agcgaaaccg
    23341 gaacgtcaac aggatcgtcg caaacctcgt cagagcaacc gccgtgaccg taatgagcgc
    23401 cgcgacaccc gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat
    23461 cgtcgccagg cacagcagca gactgccgag acgcgtgaga gccgtcagca ggctgaggta
    23521 acggaaaaag cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc
    23581 cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa
    23641 tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag
    23701 cgtcagctca atcagaaagt gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca
    23761 ccggtggttg aagaaactgc cgctgccgaa ccaattgttc aggaagctcc agctccacgc
    23821 acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa
    23881 gagaacaatg ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct
    23941 cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc
    24001 cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc
    24061 tggatccgct atccaattgt acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag
    24121 gaagaagtac aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tgtcgaacct
    24181 gttgttagcg cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt
    24241 gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcggta
    24301 actgaacagc cgcaggtgat taccgagtct gatgttgccg tagcccagga agtcgcagaa
    24361 cacgcagaac cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa
    24421 actgctgagg ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc
    24481 gctgaagtcg cagcagaagt tgaaacggta gccgcggttg aaccagagat caccgttgag
    24541 cataaccacg ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt tccggaggca
    24601 ccgcgtcaca gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt
    24661 ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa
    24721 taattagctc aaagtaatca agccctggta actgccaggg cttttttatt tcatgtttga
    24781 atcaacatat taaaattcat caaatggcat ccttgctaac caacaatgca aaataggcaa
    24841 aaaaaatgcc cgccccggcg atcccagagc gggcagacaa aacataccca gtttcatgat
    24901 atgttttaaa gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa
    24961 atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat
    25021 tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac
    25081 ctaaggaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaacggtaa
    25141 ggactttatt cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga
    25201 tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca
    25261 gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg
    25321 taatactgtc gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga
    25381 cctgttgctt aatactttct gttccgccga cataatcacc gttagcctca ctaaacggcg
    25441 cagtctctgt tttataaccg gcaaaaatgt agcgcccatt accgtcagtg gtgttcgcca
    25501 gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt
    25561 cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg
    25621 tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca
    25681 gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa
    25741 tgggatcgtc tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca
    25801 tccattctgc ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac
    25861 tgaaacgcat tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc
    25921 cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta
    25981 ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac
    26041 attaccttgc gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac
    26101 taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg
    26161 cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc
    26221 catcgcgatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac
    26281 tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc
    26341 atcaaatgcc actttaccgt tggcatccgg cgtcaccgta aaagtggtat tgctggcaaa
    26401 gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccgc
    26461 ggaggcatca gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag
    26521 aaccgcgggc ttaccgatag caaagaaatc ttcaccagca tcgccgttgg catcaaatcc
    26581 ggctttgtgt tggctgttga aagcctcggc aagtgccagc gccagttgtc caagcgtatt
    26641 acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt
    26701 cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt
    26761 acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac
    26821 cagtgagtaa ccattggcca tcgtgatgtt ataagtaccg ccatcctgaa cgctgacttc
    26881 tacgccaaca atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt
    26941 aggtgacgcc cctgccccca cgcctgtcag gcgcgagatt tgatcgttca ggctggcaat
    27001 ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt
    27061 gtcctggtcg cgcagatatt gatcggtggt tttaaactga ttcaccaacc cttctgattt
    27121 cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct
    27181 ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc
    27241 gattttcgac atctgctcat agcgggcggt caggccgcta ctttgcgttt gcgcagcacg
    27301 taactggttg gtaataaacg catcatactc acgctgcaca ccagaaacgt agacgccatt
    27361 gccaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg
    27421 ggtatatccg gcaacgttat agctggagat attattactt gccgtattta acgccgcctg
    27481 ggccgcgttc agtccgctca tggcgttatt gatcaagctg gacatggagg ttccttataa
    27541 gccttcaatt acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga
    27601 ttatcaatgt tcatgctgta ggttttgctc accttgtcgc ttatcgattt catctgctgg
    27661 atcatgttgg tgagtttacg ggcatagtga ggatcggtag cataccccgc gtcctgtagg
    27721 gcctgcgccc cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgt
    27781 gttaacagcc caacgtaatc cgacaatgct tccagatacg agctgtagac ccgaaacttc
    27841 gcttttactt tcttcgcttc gccgttttca tattcagtcg tggtgatttc agtaactggc
    27901 cctttccagt tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg
    27961 cggcggattt gccgttgccc ccaaccagat tccagtgccg cctgagcgag gatcaaatga
    28021 tgtggcacac cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat
    28081 gctttactgt cacccggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc
    28141 tgcgaaagcg cctgattttg ataacgcacc acagtttcga gcgggaattt catcggtgct
    28201 gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgtttaac catcatctct
    28261 gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa tctgctggtc atacatactg
    28321 gtatacaggc gagtgtgctc gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg
    28381 cttttcaaca tcatctgcac gaacatccct tccacctgac gggccaccgg acggatattt
    28441 gccgccggat cttcgcccgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc
    28501 gcacttgcca gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac
    28561 atcccgcact ttgtattgat tgcagtatgg acatcagatc catcggcgta gcgcccagcg
    28621 cattgagcgc gcgcaccacg ttattgaggc aggcgctgga acgtacgctt tgcagcgaac
    28681 cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt ccaccaccaa
    28741 acggtgtatc tggctggctg acattggcct gacgattaac tgttactgag agattcccct
    28801 gcgctaccgc gcagctgtcg agggtcactt cgcgattcat caccaccgaa ccggtgcgcg
    28861 agttaatcac tactttagcg tcctgcgggg tgacattaac ctgcatattc tgaatatcgg
    28921 caaggaagcg gacctgggaa ctgttgccac tcggtacgcg cacctgaata gtccgcgcat
    28981 ctaacgcggt ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg
    29041 ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccggcg ccaaactggc
    29101 tgggcaattc acgttcaata accgcaccat tggtgatccg tccaccgttc agttggttaa
    29161 cctgaacact gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg
    29221 ccagcgcata cacctgactg tcaacgccct taagcggtgt catcaacaac gtacctccac
    29281 gcaagctttt ggcatttccc atggaagaaa ccaccacatc gatggtttgc ccctgacgtc
    29341 caaacggagg aagtgacgct gtcaccatta ccgcagcgac gttttttagc tgcatattgg
    29401 tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa
    29461 acggcgtctg ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa
    29521 tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag
    29581 ccgccgtcgt gaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac
    29641 ttacattggc gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt
    29701 aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt
    29761 attgctgccg ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc
    29821 ctgattaatg gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg
    29881 gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg gcattggccc cgccctttcc
    29941 gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg
    30001 caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc
    30061 agaggagctt ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg tatcgccaat
    30121 attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga
    30181 ctggaaaata gaaccgttgg cgacgggcgt cggaccggga accggttgtg cactggtcgc
    30241 cccctgcacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa
    30301 gctggaaata gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca
    30361 gtaaaccggt gaacctgcca ccggttatgc cttagagttg cgtcagtttt tgcagcatct
    30421 gatcggtggt ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat
    30481 tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc
    30541 ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc ggaggattgc gtttcggtgt
    30601 agaggttttc gccaatgctc tccagcccgg tatcattcat aaaggtggtg agattgagct
    30661 gcccaacctg aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac
    30721 cgatggtgat acttaacgca ttcgccggaa tggtgatcgc tggctgtacc tgaaaaccac
    30781 cagccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg
    30841 atgagccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc
    30901 tgttgttggt ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg accgggcgta
    30961 cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg
    31021 gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt
    31081 tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa
    31141 ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattgctgac
    31201 aagcagagca gaagcacgcg acagtgtgaa tttcccctca ccctaaccct ctccccaaag
    31261 gggcgaggga atagtccgcg cacttttatt gcaatgtcat caatcgttca attaactcat
    31321 cgacagcagt tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg
    31381 catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt
    31441 actgccttcc agaacccccg acatcacacg caaggttgga tctgcctgca gtaccggccc
    31501 acgcgtggcc tgggtttctg cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc
    31561 gctgcccgtg gctttcacca gtttaagacg ccctactggc gcaaccgtat ttgccggatc
    31621 gcccggattg agcgccgaga ttgtgccatc ggcagcaata gtgatttccg ccccttccgg
    31681 cacagcaatt ggcccagcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt
    31741 gggatcaacc tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggtctgcac
    31801 ggccagccag ccatcctgct gcaacgcgac gtccagcggg cgcgaggtgt aatccatttt
    31861 gccgggcgtc atatctgcgc ccggcgttga cgccgtgacc aacgtgcgcg tgggcagaga
    31921 aagcccttcc actggcaccg cgcgtaaagc attcaactgc gcgcgaaaac cgggcgttga
    31981 ggcattggcc agattactgg cggttaccgc ctgttgattc agtgtctggc tggctgctcc
    32041 catcgcggta taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa
    32101 ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac
    32161 gctgggcaac gatcatattg accagttctt tactgagatc gacgttggac gcttccagcg
    32221 caccgttggt cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag
    32281 aagattgcgt cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa
    32341 agttcgccag tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa
    32401 ccgtaccgtc atcattgatt tgataactca ccagatcgcc cggtttgtag ccgttctggg
    32461 tggttgccac aatattgtta gcgccggtat tttgctgcat ggagttgagg aagctcagac
    32521 taaacgtggc gggttctgca ccgttaattg cgccggttgc gatattattc gccatcgcac
    32581 catccactaa tgtgccatta gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt
    32641 ttggatcact gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaagt
    32701 agacgctcat gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt
    32761 tatagctatc cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat
    32821 tcaggttgat ctgcatcgat gccgtggtgg tagttttcgc tgccatcagg gtattcggga
    32881 tcgaaatatt ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt
    32941 aacccgtcag ctgtaaacct tgcatattca ccaggttacg gttttcatcc agcttaaatt
    33001 gtccgttacg gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct
    33061 ggctgatagc aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagttct
    33121 gagtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa
    33181 aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa
    33241 catcgaggtt agtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg
    33301 actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt
    33361 aagtgccgag atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa
    33421 actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt
    33481 aagaaccgtt cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg aaactgtgaa
    33541 ctccggcggt cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga
    33601 tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg
    33661 tcacagcccc ttcttcactg ccggttcccg caagaacagt ggtgccgggg atcatcacgc
    33721 cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag
    33781 atccgagtgt ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg
    33841 acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca
    33901 gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc
    33961 tggtggtact gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt
    34021 tctcctttat tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac
    34081 ttcaacgttg gcctgatagc tgcgtgacgc cgacatggag ttaaccatct ccccgacgac
    34141 atcaacgttc ggcattttta cgtagccctt tgcatctgcc agcggattac ccggttcata
    34201 aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc
    34261 agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc
    34321 aggaccggtc acgctatcag cattcgccag attactggcc gccacgttca ggcgctggga
    34381 ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt
    34441 tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc taaggctcat
    34501 ctggtattgc aggctgttat cggcaaactg ggtgcgttcg cgatccatat cgacggtatt
    34561 accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag gaggcgtcag
    34621 cgcctgcgcc gggatgtgtt gcgttgaggt catcgtcagt gcaaccacac tggttgcatc
    34681 ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc gcgcctgata
    34741 accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct gggcgcgcag
    34801 attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc
    34861 gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta tcggcggaat
    34921 aaacgcaaaa tgggtcgcta tttatgccgt tgatgaccat tgcggacagg tacaattcac
    34981 gttgtagaaa tggctggggg gcgaaaatgc tggcaataaa acgtagcgtg gcgatcattg
    35041 cgatactgtt cagtccgtta agtgcggcga gcaatctcac atcgcaattg caaacctttt
    35101 ttagcgccca gctcgcgggg gtaagtgatg aggttcgtgt ttctattcgt acagcgccca
    35161 atctactacc gccgtgcgag cagccattgc tttcgatgag caataattcc cgcctgtggg
    35221 gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtac
    35281 aggccacagg aaattatgtg gttgccgcga tgcccattgc gcggggagga aagctggaag
    35341 ctggcaatgt caaactgaaa cgcggacggc tggataccct gccaccgcgt acggtgctgg
    35401 atatcaatca acttgttgat gccattagcc tgcgcgatct atcacccgat caacctatcc
    35461 agttaaccca gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg
    35521 ccagcggtga tgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg
    35581 cacagaatgc gcgggtgcgc atggtatcgg gacaggtagt cagcggcgtt gttgatgcag
    35641 atgggaatat tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat
    35701 aagcaacaca tgataaaagc gccctcaatg aggaataaac catgagtatt gatcacactt
    35761 cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacga
    35821 acagccgggc ggcaaaaaca accgcctcca ccagcaccag tgtgacgtta agcgacgcgc
    35881 aagcaaaact gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa
    35941 aactggcgat tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga
    36001 tcaacgaagc gcagcaagac ttgcagagta actgatcgta tgacacgtct tgcagagatc
    36061 ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agagcagcaa
    36121 catctctcta tggggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc
    36181 tcactgctgg cgacgctgga ttacctcgaa cagttacgca agaaagaacc caatacagca
    36241 aatagcgttg atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc
    36301 caaatgaatc aacataacgg ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg
    36361 ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca
    36421 acaacccatc gcggcggtaa aaagatttcg atctgaatat tatctccggc ctgcactgca
    36481 ggccggaatg cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac
    36541 cgccagtgca gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa
    36601 gcgccagggc atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac
    36661 gccagacatc accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg
    36721 cggagtaaag attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc
    36781 cgccagacca atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt
    36841 catcaattgc gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg
    36901 gcgggaataa aagccaggag ccaacacttt cactacaatc aggccaatca aacccaccga
    36961 gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac gcggtaaatt taccgtactg
    37021 gaacagcgaa acggttaacg gacctgaaag aatgcccaac gcaaccgcac tcggcagcgc
    37081 caacaggaaa caaagacgca acccccagtc catcaaacgg ttgtattcat catgattgcc
    37141 actggcaaaa cttttcgaca gcgatggcag caaaatggtg ccaagcgcca cgcccagcac
    37201 accggaggga aactccatta agcggtcggc gtaatacatc caagacaccg aaccggaagc
    37261 aagaaacgag gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag
    37321 gatcgccggt cccatctgtt tcaccacgcg cattgctccg gcatcgtgga agttaatgcg
    37381 cggcaggacc agcatgccga tcttcttcag gtgcggtagc tgatacacca actgcaggat
    37441 gccgccgacc gtaacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc
    37501 aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt ggcgcaaacg ccggaatcga
    37561 gaagcggttc cacgtattca gaatcgctcc caccagcgac gccagggaga taagcaagat
    37621 atagggaaag gtaatcttta gtagctggct ggtcagggca aatttgtcag ctgtgtcagc
    37681 gaagcctggc gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac
    37741 aaccgccagc gcgagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc
    37801 ttcaccctgc ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc
    37861 ttcggcaaag atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt
    37921 tgccatccct gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg
    37981 cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct
    38041 aatccagacc cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac
    38101 ctggattatg gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc
    38161 gccagatctt gtcaacgata cgttgcgcca gcactgcctg ttcgccggcg gtttgcggaa
    38221 ctgtctgatt ttgcacacat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct
    38281 caagagtgct ctgccaacca ggaatcggtt tatgcactac gccctgcccg cgctcctcac
    38341 gccattcgcg catatccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt
    38401 cacgctgact tccggcacgg cgatgcatac aggtggtgat ctgcaaagga ccagccgaaa
    38461 aatggtgctc ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca
    38521 gagaggcttt gccacccgac aaccacagcg cggtatccac cacatgcaga taatcatcca
    38581 gcaacgtgaa ataaagatcg tgtggcccga cgctattgct acgatgttta tccattctta
    38641 acgaggctgc ggtggcgagt tgcgttttta actctccgta gagtggtgcg aaacgacggt
    38701 taaaaccgac catcaacgtc agttttttcc gcgccgccag ttccaccagc cgttcagcat
    38761 cgcgcagatt ttctgccagc ggtttatcga cacagacatg tacccccgca ttgagtaacg
    38821 tactgaccac gtcaaagtgg ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg
    38881 cggcaaggct ggataacgaa tcggcataag gaatgcgcca gctttcacaa attggcaggg
    38941 ctttcgcgcg cgtaggcgac caggctcctt gtaacatcca gtcagacgct gccgccagca
    39001 ccggtaacca cgctttttgc gcaatgccgc ctaatcccac aacgccgata cgtaattttt
    39061 tcactttaat ctcccagatg ggccagcaac gaatcaagac gctgtttcag ttctgccact
    39121 tcgatttcca gggcttcgac gcgggcctgt aaatcaccat caaccgcgtt cgacatatcc
    39181 gttaccgccg gctgatcctc tacctcacca ctgaaaagat gcatgtagcg gctttcgcgt
    39241 ttacccggtt cgcgggccag acgcaccaca aaaggaccat cttcgcgatt tgccagttgt
    39301 tccagcgtcg actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg
    39361 cgcagttcac ccggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttcc
    39421 gctgcgctca gtttcaaatc gccaaattct gaattacaaa aacgttgttc atatttggtg
    39481 acccgattac caaagccact caccgtgcgc agataatggc gtttgaccag attatccagt
    39541 tgctcctgca cttcggattc actcagattc atgaccggtt cacggttcgt tttctgatta
    39601 caggccgtga ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt
    39661 tccagcaagc aaccaatcac gcgagcttca agggcagtaa gttgatattt catgagattc
    39721 tccttagcgg ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca
    39781 ttgtccatca atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc
    39841 cagtaaatca ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg
    39901 ttgggtgcgc tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc
    39961 ctgccatttt tgcccaatcg aataaccgag atagcaggca tgaaaagagc cacgaacaac
    40021 attggaaaaa ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta
    40081 gaaagctgaa ccttgtttat gaaattcgtt aatcatcccc agcctggcct gccagcctga
    40141 tggataacag tggctttcgt cgcgcactgg ctcccagggc tttaggaaat ggcgattctc
    40201 tgcgtaataa tccgcaagac gccaggcatc acgatcatgc accagacgca cgaccagtcg
    40261 gtctgtggtt aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact
    40321 ccttcccgta acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg
    40381 tgaaaagtcc aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca
    40441 aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa
    40501 tgcagaatag aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtatcgca
    40561 ggcgaggaac ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt
    40621 ctttgttgtc ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt
    40681 catggtcggt attgctctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg
    40741 cggcgcaatt gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg
    40801 cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg
    40861 cctgctctcg ggactcggcg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa
    40921 actaatccgt ccacaacaac gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc
    40981 cggtgcggtc attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt
    41041 ctgcgccaca ggggccgttt tgtttgtgct gtgtgcggcg ttcaatgcgt ggttgttacc
    41101 ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg
    41161 tgacaagcgt tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca
    41221 agtgatgctg atgctgccaa ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa
    41281 atggatgtat gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg
    41341 gagtgaaaag cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt
    41401 aagcatgatg ccggtgggca tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct
    41461 gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc
    41521 ggacgcaaga gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg
    41581 cgctattggt tatatcggtg gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc
    41641 agagcttccg tggatgatgc tgggaattat tggcatcttc actttccttg cgctgggttg
    41701 gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac
    41761 tcccccctga ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa
    41821 gctatacatt tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa
    41881 aaatatcccc gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat
    41941 ggtcggtcaa aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag
    42001 catggatatc gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg
    42061 ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt
    42121 gccgcgtttt gccaaatcgg catttgatga gttttctact cccgccgcgc gcaaatattt
    42181 cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacgg
    42241 tctgattaag aatatcagcg atgatttgcg tgcgctggac aaactgatcg tcaaacctaa
    42301 cgccgtgaat ggcgaacttt cggaagatga tattcagcta tttccgctac tgcgtaatct
    42361 gacgctggta gccggaatca actggccaag ccgcgttgct gattaccgcg ataatatggc
    42421 gaaacagaca caaatcaatt tgttatcatc aatggcgatt taatcctgac ccgccgcggc
    42481 agcctgtatt cgcgggttgc cgccgttttc agttttttct gctggcgagt ttatcgctgg
    42541 tgacacatgc tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga
    42601 acaagttttt atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac
    42661 tcacccaata caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt
    42721 tctcaaaaga tatcggttta cccggcgtgg cggacgccca tattgttctg acaaacttga
    42781 ccagccaaat tggtcgcgaa gagccgaata aggttaccct aaccggagac gccaatctgg
    42841 acatgaactc cctgttcggt agtcagaaag cgaccatgaa actgaagctg aaagcgctgc
    42901 ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga
    42961 cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ttgaaccagg
    43021 cattacgcaa ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg
    43081 gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca
    43141 ttccgtttac ggattaatga cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa
    43201 aacgtttccg cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat
    43261 taatgactgc accatcccag gtattaaaga tccgccgccc agacgactgg caccttcacc
    43321 tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac cagcgaaatt tatggacggg
    43381 ctattgtaat gcccaatctg gctccgcccg tgaccactgt tgaggctgcc gtggcgtatc
    43441 gccagcgtat tcttgatgcc gtacctgccg ggcacaattt caccccattg atgacctgtt
    43501 atttaacaga ttcgctggat cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca
    43561 ccgctgcaaa actttatccg gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa
    43621 ttgacgcaat catgccggta cttgagcgca tggaaaaaat cggtatgccg ctactggtgc
    43681 atggtgaagt gacacatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa
    43741 gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa agtcgttttt gagcacatca
    43801 ccaccaaaga tgctgccgac tatgtccgtg acggaaatga acggctggct gccaccatca
    43861 ctccgcagca tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc
    43921 tgtattgttt acccatcctc aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg
    43981 ccagcggttt taatcgagta ttcctcggta cggattctgc gccacatgca cgtcatcgca
    44041 aagagagcag ttgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg
    44101 ctaccgtctt tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg
    44161 gcccgcagtt ctatggcttg ccggtcaacg acacattcat cgaactggta cgtgaagagc
    44221 aacaggttgc tgaaagcatc gcactgactg atgacaccct ggtgccattc ctcgccgggg
    44281 aaacggtacg ctggtccgtt aaacaataaa aatcgtagct tcctgttgtc attaggttat
    44341 tttacctgta taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc
    44401 atagcgaaaa cttctccatt gccagctggg gctattgacg ccctggctgg cgaactttcc
    44461 cgccgtattc agtatgcgtt tcctgataat gaaggccacg tatcggtacg ttatgccgca
    44521 gcgaataatt tatcggttat tggcgcaaca aaagaagata aacagcgcat tagcgaaatt
    44581 ctccaggaaa cgtgggaaag cgccgatgac tggtttgtca gcgaataata tgcagtgatt
    44641 tttgtttttg ccgggtcgcc ccggcttttt tattttactt acaaattttg agtac
//
LOCUS       DAJSKJ010000012        42710 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000012 DAJSKJ010000000
VERSION     DAJSKJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.012"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(200..307)
                     /locus_tag="ON345_000584"
     CDS             complement(200..307)
                     /locus_tag="ON345_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR3070695.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(683..790)
                     /locus_tag="ON345_000585"
     CDS             complement(683..790)
                     /locus_tag="ON345_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR3070696.1"
                     /translation="MTLAELGMAYWHDLAAPVIAGILASMIVNWLNKRK"
     gene            1266..2537
                     /locus_tag="ON345_000586"
     CDS             1266..2537
                     /locus_tag="ON345_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR3070697.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(2567..3571)
                     /gene="dppF"
                     /locus_tag="ON345_000587"
     CDS             complement(2567..3571)
                     /gene="dppF"
                     /locus_tag="ON345_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR3070698.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(3568..4551)
                     /gene="dppD"
                     /locus_tag="ON345_000588"
     CDS             complement(3568..4551)
                     /gene="dppD"
                     /locus_tag="ON345_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR3070699.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(4562..5464)
                     /gene="dppC"
                     /locus_tag="ON345_000589"
     CDS             complement(4562..5464)
                     /gene="dppC"
                     /locus_tag="ON345_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR3070700.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(5474..6493)
                     /gene="dppB"
                     /locus_tag="ON345_000590"
     CDS             complement(5474..6493)
                     /gene="dppB"
                     /locus_tag="ON345_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR3070701.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6801..8408)
                     /gene="dppA"
                     /locus_tag="ON345_000591"
     CDS             complement(6801..8408)
                     /gene="dppA"
                     /locus_tag="ON345_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR3070702.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(9153..9229)
                     /locus_tag="ON345_000592"
     tRNA            complement(9153..9229)
                     /locus_tag="ON345_000592"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9193..9195),aa:Pro,seq:cgg)
     gene            complement(9321..11012)
                     /gene="eptB"
                     /locus_tag="ON345_000593"
     CDS             complement(9321..11012)
                     /gene="eptB"
                     /locus_tag="ON345_000593"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR3070703.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            complement(11336..12544)
                     /locus_tag="ON345_000594"
     CDS             complement(11336..12544)
                     /locus_tag="ON345_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3070704.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            complement(12773..13516)
                     /locus_tag="ON345_000595"
     CDS             complement(12773..13516)
                     /locus_tag="ON345_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR3070705.1"
                     /translation="MTIILPNSALPWSRVNDHKKSGGRWQLSLLASVVISAFFLNTAY
                     AWQQEYIVDTQPGHSTERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDV
                     TSSMSMGWNFPLYEQVTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGW
                     RVDSRLGDLRPWAQISYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQN
                     IAAYAALSQAENTTNNSDYLYTMGVSARF"
     gene            13628..14191
                     /gene="tag"
                     /locus_tag="ON345_000596"
     CDS             13628..14191
                     /gene="tag"
                     /locus_tag="ON345_000596"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR3070706.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            14188..14628
                     /locus_tag="ON345_000597"
     CDS             14188..14628
                     /locus_tag="ON345_000597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3070707.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(14597..16930)
                     /gene="bisC"
                     /locus_tag="ON345_000598"
     CDS             complement(14597..16930)
                     /gene="bisC"
                     /locus_tag="ON345_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR3070708.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHLQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQLLAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            17083..17742
                     /locus_tag="ON345_000599"
     CDS             17083..17742
                     /locus_tag="ON345_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR3070709.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            17846..18820
                     /gene="ghrB"
                     /locus_tag="ON345_000600"
     CDS             17846..18820
                     /gene="ghrB"
                     /locus_tag="ON345_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR3070710.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(18870..19580)
                     /locus_tag="ON345_000601"
     CDS             complement(18870..19580)
                     /locus_tag="ON345_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR3070711.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            20014..20304
                     /locus_tag="ON345_000602"
     CDS             20014..20304
                     /locus_tag="ON345_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR3070712.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            20585..20797
                     /gene="cspA"
                     /locus_tag="ON345_000603"
     CDS             20585..20797
                     /gene="cspA"
                     /locus_tag="ON345_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR3070713.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(20985..21137)
                     /gene="hokA"
                     /locus_tag="ON345_000604"
     CDS             complement(20985..21137)
                     /gene="hokA"
                     /locus_tag="ON345_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR3070714.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(21463..23532)
                     /gene="glyS"
                     /locus_tag="ON345_000605"
     CDS             complement(21463..23532)
                     /gene="glyS"
                     /locus_tag="ON345_000605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR3070715.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFTEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(23542..24453)
                     /gene="glyQ"
                     /locus_tag="ON345_000606"
     CDS             complement(23542..24453)
                     /gene="glyQ"
                     /locus_tag="ON345_000606"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR3070716.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(24548..24847)
                     /locus_tag="ON345_000607"
     CDS             complement(24548..24847)
                     /locus_tag="ON345_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR3070717.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            25022..26017
                     /gene="wecH"
                     /locus_tag="ON345_000608"
     CDS             25022..26017
                     /gene="wecH"
                     /locus_tag="ON345_000608"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR3070718.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(26059..26496)
                     /locus_tag="ON345_000609"
     CDS             complement(26059..26496)
                     /locus_tag="ON345_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR3070719.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(26542..26883)
                     /locus_tag="ON345_000610"
     CDS             complement(26542..26883)
                     /locus_tag="ON345_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070720.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(27052..28506)
                     /gene="xylB"
                     /locus_tag="ON345_000611"
     CDS             complement(27052..28506)
                     /gene="xylB"
                     /locus_tag="ON345_000611"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR3070721.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(28578..29900)
                     /gene="xylA"
                     /locus_tag="ON345_000612"
     CDS             complement(28578..29900)
                     /gene="xylA"
                     /locus_tag="ON345_000612"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR3070722.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            30266..31258
                     /gene="xylF"
                     /locus_tag="ON345_000613"
     CDS             30266..31258
                     /gene="xylF"
                     /locus_tag="ON345_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3070723.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMVNDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            31336..32877
                     /gene="xylG"
                     /locus_tag="ON345_000614"
     CDS             31336..32877
                     /gene="xylG"
                     /locus_tag="ON345_000614"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR3070724.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEINHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRNG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            32855..34036
                     /gene="xylH"
                     /locus_tag="ON345_000615"
     CDS             32855..34036
                     /gene="xylH"
                     /locus_tag="ON345_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR3070725.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            34114..35292
                     /gene="xylR"
                     /locus_tag="ON345_000616"
     CDS             34114..35292
                     /gene="xylR"
                     /locus_tag="ON345_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR3070726.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(35400..36224)
                     /locus_tag="ON345_000617"
     CDS             complement(35400..36224)
                     /locus_tag="ON345_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR3070727.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            36544..38574
                     /gene="malS"
                     /locus_tag="ON345_000618"
     CDS             36544..38574
                     /gene="malS"
                     /locus_tag="ON345_000618"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR3070728.1"
                     /translation="MKLAAYFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            38752..40005
                     /gene="avtA"
                     /locus_tag="ON345_000619"
     CDS             38752..40005
                     /gene="avtA"
                     /locus_tag="ON345_000619"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR3070729.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(40156..40629)
                     /locus_tag="ON345_000620"
     CDS             complement(40156..40629)
                     /locus_tag="ON345_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR3070730.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(40731..41579)
                     /gene="yiaJ"
                     /locus_tag="ON345_000621"
     CDS             complement(40731..41579)
                     /gene="yiaJ"
                     /locus_tag="ON345_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR3070731.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            41780..>42710
                     /gene="yiaK"
                     /locus_tag="ON345_000622"
     CDS             41780..>42710
                     /gene="yiaK"
                     /locus_tag="ON345_000622"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR3070732.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTL"
BASE COUNT        10659 a        11106 c        10513 g        10432 t
ORIGIN      
        1 ttccggcttg acgctgattc tacgactgtt tttctgttcc ggaatccgtc acgtcttcac
       61 tctgtgctgc gcattccgca gaatattatc cgcttgcaac cgttgcattt ttcttacgaa
      121 aaaggccgca gagtttcacc cctgcggcct gatccgggcg caaattgcca ttacggcagc
      181 ctgacgcccg catggcacgt tacttccgct tgttcagcca gttcacgatc atactggcaa
      241 gaataccagc aatgaccgga gccgctaaat catgccagaa ggccatgccc agttctgcga
      301 gcgtcatata gccgcctgta ttgtaatgac aacgtttcgc ggctattctt gtattgtttg
      361 gatgcaagaa tggcccccgt ggtgttgtca ggtgcatacc tgcaacgtgc gggggttttc
      421 tctctccagc aaccaatgcc accagggatt aagcccccgc aacattgcgc ctcaccggaa
      481 tccttccggc ttgacgctga ttctacgact gtttttctgt tccggaatcc gtctcatctt
      541 cactctgtgc tgcgcattcc gcagaatatt atccgcttgc aaccgttgca tttttcttac
      601 gaaaaaggcc gcagagtttc acccctgcgg cctggtccgg gcgcaaattg ccattacggc
      661 agcctgacgc ccacatgaca cgctacttcc gcttgttcag ccagttcacg atcatactgg
      721 caagaatgcc agcaatgacc ggagccgcta aatcatgcca ataggccatg cccagctctg
      781 cgagcgtcat atagccgcct gtgttgtaat gacaacgttt cgcggctatt cttgagtggt
      841 ctaggttcaa gattagcccc cgttatgttg tcaggtatat acctgcaacg tgcgggggtt
      901 ttctctctcc agcaaccaat gccaccaggg ataaagcccc cgcaacattg cgcctcaccg
      961 gaacccttcc ggcttgccgc tgattttacg attgcttttt actgtcagca cccggcaatc
     1021 ttcctcattg cgcgatgcct tccagcttaa tttctttatt tgcaatagtt gcagttcata
     1081 tccgggctga ctccagactg gtagtgaaat gcgcaatttt gtagaattgc gcctcctcgc
     1141 gacctcccca gtgaatctgt tgcctcattt tttacatact gcgtattcga cttctccacc
     1201 tgttgcgcaa gagaaactgg gtttattcat ttttgcgagg ccgacttctt tctggacagg
     1261 actttatgca gcacaacaca ctatcgaaac acaatcagaa attgccgttt acacgctacg
     1321 acttcggctg ggttttatta tgcataggca tggcgattgg tgccggaacc gtgctgatgc
     1381 cagtacaaat tggcttgaag ggaatttggg tatttattac cgcagcgatc attgcttatc
     1441 ctgccacctg ggtagtgcag gacatttatt taaaaaccct ttctgaaagc gattcctgta
     1501 atgactacac cgatattatc agtcattacc tggggaagaa ctggggaatt ttcctcgggg
     1561 ttatctactt tttgatgatt atccacggga tttttatcta ctctctctcc gtggttttcg
     1621 acagcgcctc gtacctgaaa accttcggtt taaccgatgc cgatctttca caatctctac
     1681 tttataaagt cgctattttc gccgtactgg tggcgattgc gtctggtggt gaacgattac
     1741 tgtttaagat ttccgggcca atggtggtgg tcaaagtagg gattattgtc gtgttcggtt
     1801 ttgcgatgat cccgcactgg aatttcgcca atataaccgc cttcccgcaa gcctccgtct
     1861 ttttccgcga tgtcttgctt accattccat tttgcttctt ttctgcagta tttattcagg
     1921 tacttaaccc aatgaatatt gcctatcgta aacgggaagc ggataaagta ctggcaaccc
     1981 ggctcgcgct gcgtacccac cgaattagtt atatcacgct catcgcggtg atcctgtttt
     2041 ttgccttttc gtttaccttc tcaattagcc acgaagaagc cgtttctgcc tttgaacaaa
     2101 atatctcagc actggcgctg gccgcgcagg tgatccctgg gcatatcatt catatcacct
     2161 ctacggtgct taatatcttt gccgtactga ccgcattctt tggcatttat ctcggtttcc
     2221 acgaggccat taaaggcatt attctcaatc tgttaagccg aattattgat accaagaaaa
     2281 ttaactcacg cgtgctgact ctggcgatct gcgcttttat cgtcattacg ttgacgattt
     2341 gggtttcgtt tcgtgtatcg gtgctggtgt tctttcagtt gggaagcccg ttatatggta
     2401 ttgtgtcgtg cctcattccg tttttcctga tctataaagt cgcacaactg gaaaaacttc
     2461 gcggatttaa agcctggctg attctgctgt acggcatttt gctatgcttg tcgccactgt
     2521 tgaagctgat tgagtaaacc ggagcgcatg gccccggttt tgtgagttaa cgctgcggat
     2581 tttcatcctg atcaacagca aaacaagcta ccagttgacc gccgtagtct tttagctgcg
     2641 gctgcaactg ggtgcagggg ccgaagcgcc gacgacagcg ggcgttgaag gcgcaacccg
     2701 gcggtggatt cagtgggctt ggtagttcac cgctgagctt gatgcgctcg cggcgatcgt
     2761 ccgggttcag gcgcggcgtc gcggaaagta gcgcctgagt gtacggatgg cgcgggttat
     2821 tgaagatttg gtctttcgtt cccttctcca cgcagcggcc caggtacatc accatcactt
     2881 catcagcaat gtgctccacc accgacaggt cgtgggagat aaagacataa gacagcccca
     2941 actcctgctg caaatccatc atcagattca gcacctgcgc gcgcactgaa acatccagcg
     3001 cggaaaccgg ttcatcggca atcaccacat ccgggtcgag catcagaccc cgggcgatgg
     3061 cgatacgctg acgttgtccg ccggaaaaca tgtgcggata gcggtcatag tgctcggttt
     3121 tcaggccgac tttcgccatc atcgacaggg ctttttcccg acgctgttct ttgcttaagc
     3181 tggtgttaat aagcagcggc tcttcgagaa tttgcccaac tttcttacgc ggattcagcg
     3241 aaccgtaggg attctggaag acgatctgga ttttctgccg acgcagcttc tgcgcctgcg
     3301 gatcgtgctt gagcagatcc tgcccctgat aatacagctc gccgccggtg ggcgtttcga
     3361 tcatcgtcag caaccgaccg agggtcgatt taccgcagcc agattcgccc actaccgcca
     3421 gcgttttgcc acgttcaagg ttaaacgaaa cgccatccag cgccttcacc agacgttccg
     3481 gcgcaaacat ccctttcttc accggataat gttttttcag gtcaatcgcc tgcaacagcg
     3541 gttgttgcga ggtggcctca tgcgtactca tagtgtgggc ctcccggcat catcaagtgg
     3601 gtaatggcat ttggactgac gcccgtcagc gagcatattc agcgccggtt cttcagcgcg
     3661 acatctgtca gtggcatagg ggcagcgcgg gttaagcagg cagccgttcg ggcggtcgta
     3721 cttgccggga acgacacctg gcaacgatgc cagacgttct ttgtcctgag caaattctgg
     3781 cagcgcacgc agcaatgcct gagtatacgg gtgacgcggc gcatggaaga tggcgtgcgc
     3841 atcaccggtt tccaccacct ggcctgcata catcacgatg attttatgtg ccgcttccgc
     3901 caccagcgcc aggtcatggg taattaacac cagcgccatg ttctctttct gctgtagctc
     3961 cagcagtagt tcgatgattt gcgcctgaat ggtcacgtcc agcgcggtgg tcggttcatc
     4021 ggcaatcagc agttttggcc gacaggcaat cgccatggcg atcatcacgc gctggctcat
     4081 gccgccggaa agctgatgcg ggtaaacatc cagacgcgat gccggatcgg gaataccgac
     4141 ctgattcagc aggtcaatcg ctcgctgacg gcgggtactt ttgttgccgc cctgatgcac
     4201 cttaatcgct tccataatct ggaaacccac ggtgtagcac gggttaaggc tggtcatcgg
     4261 gtcctggaag atcatcgcca cttcggcacc caccaggttg cggcgctctt tttctgagat
     4321 acgctgcaaa tcctggccgt taaactccag tttttctgcc attacgcggc ccggataatc
     4381 aatcagcccc ataatcgcca gtgaactgac cgacttaccg gagccggact cacccacaat
     4441 cccgaccact tcgccctgtt ttacgctgta gctgatgcgg tctacggcgc ggaacggcgc
     4501 gctttcgtcg ccgaaatgca ccgataattt atctacattt aataacgcca tctcgaacct
     4561 cttactgctt cagtttggga tcgagcgcgt cacgcagacc gtcacccatc aggttaaatg
     4621 ccagcaccgt cagcaggatc gccagacccg ggaaggtcac gacccaccag gcgctttgcg
     4681 cgaactgcaa cacgtcggag agcatggtgc cccactcagg cgttggcggc tgtgccccca
     4741 tgccgaggaa accaagagca gccatatcga gaatggcgtt agagaaaccg agcgacgcct
     4801 gaacaatcag cggcgcaagg cagttcggga agatgttaat aaacatctga cgcatcgccc
     4861 cggcacccgc cacgcgagac gcggtgacgt aatcgcggtt aacttccacc agcacggcgg
     4921 cgcgggttaa gcgcacatag tgcggcaagg caacgaaggt cagtgccagc gcggcgttac
     4981 caatcgacgg gccgaaaatt gccaccagca ccagcgccag cagcagactt ggcagcgcca
     5041 gcatgatatc gaccacgcgc ataatgatgt tatcgaccag gccgccaaag taaccggcga
     5101 tcaggccgag aataacgccc ataatcagcg ataacacaac taccagacag ccaaccagca
     5161 gcgacaggcg cgcaccgtac atcaggcgcg acagcacatc acggcctacg tcatcggtgc
     5221 ccagcaagtg cgccatgctg ccgccttcct gccaggctgg cggggcgagc agtgcatcgc
     5281 ggaactgttc cgccgggtta tagggtgcaa tccagttggc aaagatcgcg atgaacagca
     5341 cgatgacgac gtaaaccagc ccgacgaccg cgcctttgtt gcgtttaaaa tagtgccaga
     5401 actcctgtaa cggggtcatc ggcaccggtg cgctaatcac tttattttca gtaacctgtg
     5461 acatgatgtc cccttacttc ttatgacgaa tacgcgggtt caccacgccg tacagcagat
     5521 cgaccagcaa gttgacgagg ataatcatcg tcgccaccag caatacgccg ccctgcacta
     5581 ccggatagtc gcggcgttgc agtgcgtcaa tcaaccagcg tcccagaccg ggccacgaga
     5641 agatggtttc ggtcagaatc gcccccgcca gcaatgttcc cacctgcagg ccgataacgg
     5701 tcaccaccgg cagcatcgcg ttacgcagcg catggacgat aatcacccgc atgcgggtta
     5761 gccctttggc gcgcgcggtg cggatgtaat cctcgcccag cacttccagc atcgaggagc
     5821 gtgtcatacg cacaatgacc gccagcggaa tagtacccag cacaatggcg ggcaagatca
     5881 tatgggcgac ggcatcgata aagttgccgt cttcacccca gatggcggtg tcgattagca
     5941 taaaaccggt taacggattg gagtcatcga ggaacaccat atcgctcacg cgaccggaga
     6001 cgggcgtcag gttccagtgc accgaaacca gcatgatcag catcatgccc caccagaaga
     6061 tcggcatcga gtagccggtc agcgccaggc caactgcagt gtgatcgaaa atggaaccgc
     6121 gtttaaccgc agccagcaca ccaaccggaa taccgactgc cgtagcaaaa atcatcgcgc
     6181 agacgccaag ttccagcgtg gcctgaaagc gcggcacgaa ctcttcccat actggaattc
     6241 ggcttttcat tgaaatgcct agatcgccgt gcattacgcc ccaaatgtaa tggagatact
     6301 gctgccacat cggtttatct aagccgagtt cagccagcag ctgcgcgtga cgctcagggg
     6361 agatcccacg ttcgcctgcc atgatcatca ccggatcgcc cgggatcatg tggacaaagg
     6421 caaatgtgag aagggtaata ccgataaacg tggggatgac gagtcccaaa cgtcggagaa
     6481 taaactgcaa cataacccgg attctctata gtgacccgca gcctggaact gcgtgtctgt
     6541 attgctcaca aatcaattcc cgcccttgtc ggacagagaa tgaagtattg ctgccggatg
     6601 gcgctgcacg cagaggcaca gcgccaccgg gtatacagat tgttgacaaa gtgcgctttg
     6661 tttatgccgg atgcggcgtt aacgccttat ccggcctaca aaatcgtgca aattcaatac
     6721 attgcagaat tttcgtaggc ctgataagcg gagcacatca ggcagttttg cctttgccat
     6781 cagtcttgta tggcttttaa ttattcgata gagacatttt cgaagtgatg tttgcctaat
     6841 ggatcaacca catagccttt gacttctttg cgtaccggtt caaacacggt ggagtgagcg
     6901 atgatcagtg ccggagcctg atcgtgcatc accacctgcg cttgcttgta cagttcaacg
     6961 cgtttgttat ggtcgtcggt agcacgcgcc ggttgaatca gatcttcaaa cggtttgtag
     7021 caccattttg agtagttaga accttgttca gaggcggcgc agctgaacag ggtggcgaag
     7081 aagttatccg ggtccccgtt atcgccagtc cagcccatca ttaccgtctg atgttcgcca
     7141 tctttcgcac gcttgaggta ctcaccccat tcgtaggtga cgattttggc ctgcacgccc
     7201 actttcgccc agtctgcctg aatcatctcc gccatgcggc gagcgttcgg gttatacgga
     7261 cgttgtaccg gcatcgccca caggtcgatg gagaaacctt tttccagacc cgcttctttc
     7321 agcaaggctt tcgctttttc aggatcgtag gtgtaatcct gaacgtcgtc gttatagccc
     7381 cacatggttg gcgggatcag gtttttcgct gatacacccg cgccctgata aaccgctttg
     7441 atgatcgcgt ctttgttcac cgcgtaggtc agtgcctggc gaactttcac gtcatccagc
     7501 ggttttttct gcacgttata ggagaggtag ccgacgttca gccccggcat ttccatcagg
     7561 ttgatggatt tatcctgctt catgcgagcg atgtctgccg ggttcgggta cggcatcacc
     7621 tggcattcat ttttctgcaa tttcgcgtaa cgcacggaag catcaggggt aatagagaaa
     7681 accagcgtat cgatctgcgg tttggtgccc cagtagccat caaacgcttt gtagcggata
     7741 cgggaatctt tttggtactg ctgtaactgg aacggaccgg ttccgattgg gttgaggtcc
     7801 agtttttccg gtgtaccggc tttcatcatc gcgtcagcat attcttttga cagaatagag
     7861 gcgaagtcca ttgccaggtc agcgaggaac ggcgcttccg gtcgagtcag cacaaactga
     7921 actgtgttgt cgtccacctt tttcacttcg ctgatcagct ccggcaagcc catgccttcg
     7981 aagtattcgt agctgccgcc agagacttta tggtacgggt tttgcgcgtt tttctgacgg
     8041 tcgaacgaga acaccacgtc atcggcgttc agttcacgcg tcggtttgaa ttctttattg
     8101 tcgtgccact tcacaccttt acgcagatgg aaggtatagg ttttaccgtc ttcgctgact
     8161 tcccactttt cagcgaggcc cgggatcact tcagtggtgc cgattttaaa ttcaaccagg
     8221 cggttataaa gcggtacgga agaggcgtca taggtggtgc cggaggtaaa cagctgcggg
     8281 ttaaaccctt ccggagatcc ttctgagcaa taaaccagag ttttagcctg aacacttgct
     8341 gcgacggtca tagccaccag gctgagacca agcttcagca tccctgactt tttcaaggaa
     8401 atacgcatta ttctgctcca attgtgatgt ttgttgtttt aaccctttgc agtgggttgt
     8461 cgctgcctga ccttttttgt tttttgcccg gtcgggtcaa cgttatgagg tggggatgcc
     8521 gtactaatta acatcagtgt aacagtgcgg atcctccata aaatgcccct cgtgacctac
     8581 aatctgtcaa cagaatgtga aaacgtcaat acagccaacc gggatttaca ccaacggtga
     8641 gaatccacaa acaaagatta aaaaaacttc aaacagctat ttgcagcaga gaaatttgtg
     8701 ctactccaac atgaccagaa caatcagctt aatatttagc aacatttggt gattaagttt
     8761 tatgcattta atgaaaaaaa tctgaggaaa aggtggatat ctgagcgatt aataccatga
     8821 acgctaaaac gcacagcgga aaatgccagc gccagccata agtaacgcaa aaaaagattt
     8881 aagcaaatat aaaaaaagac aatggtttct gtcacaaatc cgttaatgga tagtgagata
     8941 tggggcgcaa agttggcagg aaaatgtgtg gtgtgttggt cgaataaggc ggtcgcgccg
     9001 catccgacaa taagtgcggt gccgttgcct gatgcgacgc tggcgcgtct tatcaggcct
     9061 acgtcgagtc gaacctcccc gcccccggag gttctcatcc tcatgtgctt caaatgcaaa
     9121 aaagcctgct cgctgagcag gcttttcgaa tttggtcggt gatagaggat tcgaacctcc
     9181 gaccccttcg tcccgaacga agtgcgctac caggctgcgc caatcaccga atgcggggcg
     9241 catcttactg cgcagatacg ccctcgtcaa tcccttaata gcaaaatgcc ttttgatcgg
     9301 cgagaaagtc agcaggcctc ttagttagcc gctgcctctt ttgtctgcgg gatgtgacac
     9361 cagttgttgt tttcgttaat tccaccatcc ggtgaagtat agccaagaca acccatgatg
     9421 gtgtcgtaca gctctacgtg acggcgtggc actttcatgt cggcttcttt tttcagctgc
     9481 gcaaacgcct gcgcattggc cggattttcc agatatttat ctgacatcca gaccatcatc
     9541 ggtacgcgga actgctccgg cggtgccagt tcacgcggcg tgccgtgcag gtgctcgcgt
     9601 tcattaattg actcaccgtg gtcagctgcg tagaacacaa tcgctttctt atcgcgaacc
     9661 tgatcaatca cgctggagat aaagtgatcc acataggtca ccgagttgtc ataggagttg
     9721 atcatctgcg ctttggtaca gccgctatca acaccaatac attccggctt ccactgcgcg
     9781 aagctacgcg gataacgctg ggtgtagtta aaatgcgaac ctttggtatg cagaatgatc
     9841 agatgcttac catccgggtt gcgccctagc gattgctgca tttcgtctac cagcaacata
     9901 tcatctaccg gcttgccacg attacgtggc tccgcaccaa tctgctcacg ataagcaatg
     9961 ttgtccgcca tcgtgttgct gtagaaccac atttcgctct gcatagcgta gaggtcagaa
    10021 ctgaatccta actgcttcag aaccgcgaaa atgttctgtt cttttaatgt gcgctgcgga
    10081 ttatcttccg cgcccccctg acgtacaaac atgcaacgca gtgagagttt ggttgcggta
    10141 tcacaggagt aaccacggaa cgccgccaga tttttctcct gggccagttt cggcgtggta
    10201 ttacgctcat agccgaaaat acccatatgg tcccaacgcg tggtttcacc gatgataaac
    10261 acgacataag tgtcatcaac gttttgcggt gcctgataag tgaatttctt cgccggatta
    10321 agcaatgaat tattatcgga agattcatcg acccgcgccc aggcatacag ccccaacgca
    10381 gaaagccagt ttgatggcag ataagagttc gctacgacac cgccataact cggcaaatca
    10441 acgccggtcg ccctctccac tttcttctgc tggatatcca gcaaacgaat cggtgcccaa
    10501 accataatac ccgccagtac gacgaccgcc aggctgcgaa tacgctgccc tggggttcgc
    10561 agttgtcgga gcaaggtgta gcgacagcgg ttattccaga taaggataag aggcaatgca
    10621 ctaacggcga ttaaccagag aataaagttc agaccaacaa cttcttttga caggtcgata
    10681 tcggtggtca tgacggaagc gatgatgcca taaccaatga ccacattaag gaaggtcatg
    10741 taatagctgg cacctgcgga aaagagcacc accagcgacg ccagaatacg ccagctgcgg
    10801 cggccaaaca gcgatagaag acgtagtaaa aagaaggtca ccagtacggt ggcggccagt
    10861 tcaacaacag cagaaacgcc tttccagacg gtaaaatcgt gcgcatagct gccgaagcgg
    10921 cgataaaaaa ccgcgccatt cataaaaagg ccaatataga ttgcaagcaa aaagctcagc
    10981 ttctgctgtg taatcgattt gatgtatctc atgcaaacaa accctggaca aacaggtgat
    11041 aacaccctgc ttagcggctt ttcaggcccg gcaggggaaa agtgcggagg tagtcagata
    11101 agtaaaatct acacgcgcga ataagtagac cacagcaaag cgaaaaagtg tggggaaaga
    11161 gtgtgcatga agcacaaatt tacaaaaatg caagtttgaa aagagacttt agcgttacaa
    11221 aaagcagtaa aaagggccag ttaactggcc caaattttac gagtcattgt ttatacattt
    11281 cgttgatgcg tttgatgcac acggaagctg aagcccagta gctcgcggct gagcattaaa
    11341 gggagccatg cgcctcacgc aacattttct gctctggctg acgaatcgtc gtagaaagcg
    11401 ccaatgacag aatcagcagg gcgaaaatca cgtagaaggt cacatagaaa ccgccaaaca
    11461 gtgaggcgat aatcgaacca aaaatgctac cgataccgaa accgaggtaa atcacaccgt
    11521 agtttttcgc caggttattg aggccgaaga actcactgac cagtgatgga aagacggtaa
    11581 tagtgccgcc aaagttaaat gccacgcagg caatcgctgc aaagaacgtc actgcattta
    11641 atggtgcaaa cagcagggcc gccataccca ccagcgaaat cacctgacca atggtgataa
    11701 cacggatacg ggcaattttg tcagacagaa tgcccagcac cagacgacct gaaaggttag
    11761 cgatggaaat aaccgttact gcattggctg cggaaaccac atcaaggtgt gccagacttt
    11821 gggcgatatc tttcgctacc ccaatcacgt acaggccgct catgcaggca gtcaggaaca
    11881 ttaccgctaa catccagtac tgcggtttac gcatcgactc tgccagcgtg taatctttct
    11941 ccaccacacc attgctggtt ttcacttcct gttttggtgc gtctttcatt aacgttgcgc
    12001 cgaaaacaat catcaccagc gcaatcgctc cccaaatcac aaaggttttt tccagaccca
    12061 ccgtttcgag cagctgcgtg tcgataaatt tgaaacccag gctacccaga ccataagaac
    12121 cgatagcgaa cgcggagatc agacctttac gctccgggaa ccacttcacg cagttagaga
    12181 gcgtcagcag gtaacccgcg ccatccgcca gaccgaccag cacaccggcg cttaaccaca
    12241 gcatcatcag gttgttggaa tgcgcggtca ggaagaagcc taatcccagc aaaatgccgg
    12301 aagccatggt gacgcgttta acgccaaaac gttcctgcaa ttttcccgca acagaagacg
    12361 aaattgccag ccccagactt aacaagccga aagagaaagc gacctggctt accggcgcat
    12421 ccagcttggc agaaagcgcg ccattaaaca ggctccaggt ataaaccgac cccagtgcaa
    12481 actgggtaat gatagtaccg atgagtgtca gccagcgggt acgctgataa tttgaaggtg
    12541 tcatggcagt attcctgcag taataaaaag gaaaaatctc tgccgacaac cataacgaag
    12601 tgcattttat gcctggggaa aaacggcatg aaatgccatc agaacggaat gaaatgcctt
    12661 agttcaggaa tgaatgacgc gacaattatt caccaaattt attatttagt aagttagcac
    12721 ccactatcgc cacaactgtt gttatccaat ggtttcaatt gtgattttta cgttaaaatc
    12781 tggcactaac ccccatcgta tacagatagt cgctattgtt agtggtattt tctgcctgag
    12841 atagcgcggc ataggcggca atattttgat tgagcaacat atcagcgcct acggtgacat
    12901 ccagccagtt gccgttctgg tttgtcgccg tcatccggct caggcctgat tgcgccttcc
    12961 agatattctc gccaaattgc tggttatagc tgatttgcgc ccagggtcgg agatcgccaa
    13021 gccgggagtc aacacgccag cctaagctac tcacgctggc atgccataac ggatcggtca
    13081 gcgtggtagt actgtcgcca aactcgttat acatcgaggt ggttgtgcca tcgtaatgta
    13141 atgccgcgac cgggccggtt gtaacctgtt catataaagg aaaattccag cccatactca
    13201 tactgctggt cacatccaca ggagtttctt ccgccagatt gacttccagt cccagcgccc
    13261 tttggctact ttgaatacgt tgcgacaaaa tatcgttgta atcaggttga tgatcactat
    13321 cccaggtgta acgctctgtg gaatgtccgg gttgcgtatc aacgatatat tcttgttgcc
    13381 aggcgtaagc tgtgttgaga aaaaaggcac tgattaccac gctcgccagc aggcttagct
    13441 gccagcgacc accgcttttt ttatgatcat taacacgact ccagggaaga gccgaattcg
    13501 gcaatattat tgtcattgta tgaaggatat cgggcatagt agccctatat taaatattga
    13561 ctttttcacc gatgcgtcaa gaaaagcggc tgaaattttt acgatcgggt acatagcgag
    13621 ggaaagtatg gaacgttgcg gctgggtgag tcaggacccg ctttatattg cctaccatga
    13681 taatgagtgg ggcgtgcctg aaactgacag taaaaaactg ttcgaaatga tctgccttga
    13741 agggcagcag gctgggttat cgtggatcac cgtcctcaaa aaacgcgaaa actatcgcgc
    13801 ctgctttcat cagttcgatc cggtgaaggt tgccgcaatg caggaagagg atgtcgaaag
    13861 actggtacag gacgccggga ttatccgcca tcgagggaaa attcaggcaa ttattggtaa
    13921 tgcgcgggcg tacctgcaaa tggaacagaa cggcgaaccg tttgccgact ttgtctggtc
    13981 gtttgtaaat catcagccac aggtgacaca agccacaacg ttgagcgaaa ttcccacatc
    14041 tacgtccgcc tccgacgccc tatctaaggc actgaaaaaa cgtggtttta agtttgtcgg
    14101 caccacaatc tgttactcct ttatgcaggc atgtgggctg gtgaatgatc atgtggttgg
    14161 ctgctgttgc tatccgggaa ataaaccatg attcgggaag cgcaacgttc agaactcccc
    14221 gcgatcctcg aactgtggct ggaaagtaca acctgggggc atccctttat aaaagcgaat
    14281 tactggcgtg actgcattcc gctggtgcgg gatgcctatc ttgccaacgc gcaaaactgg
    14341 gtctgggaag aggacggtaa gcttctcggt tttgtcagca ttatggaagg ccgatttctg
    14401 gcagcaatgt ttgtcgcacc gaaggccgtc agacgcggta ttggtaaggc gctgatgcag
    14461 tatgtgcagc agcgctatcc ccatctgatg ctggaggttt atcaaaaaaa tcaaccggcg
    14521 atagattttt accgtgcgca gggttttcac attgtcgatt gcgcatggca ggatgaaacc
    14581 caactaccca cctggattat gagctggccg gtggttcaaa cgctgtaagt gtcagttccg
    14641 gaccgttgta tttttccagc catgccagcg ccgtattacc cgcacagcca ttccccagcc
    14701 gcgagctggg gagatctttg gtcagcacgt tcactgcgcc gtttttacaa ataccgtcag
    14761 cggttaaatc cagatccggc catgcccctt cgtgaatgca aatcacgcca ggtttaattc
    14821 cctcgctaat gaccgctccg gcaaggagct gcccacggga gttccacacc cgcaccatat
    14881 ccccttcggt tatgccgcgc gcctgagcat cgtcaggatg aatggtgaca ggctcacggt
    14941 ttgctaccgc gtacaattcg cgcagagaac tgtaattcag ctggctgtgc aggcggtgcg
    15001 ccggatgggc agaaagtacc tgcaactgtt ctggttcggc attgccctgc cattcgtccg
    15061 gctccagcca cattggatgc ccagggcaat ccgggtaacc gtaatcggca atacgctgtg
    15121 agaagatttc aatcttgccg ctggcggttt ttaacggatg cgccagcgga tcgcggcaaa
    15181 aatcagcgaa gcgaataaac cgctcgctgt ccgggttttc cggcatctca attaactggt
    15241 tggcttgcca gaactcagca aatggcggca attcaacctg ctggcttgcc ccgcgctgcc
    15301 gggcaacgtt ataaaacgtt tccagccatt gcagctgact ttttccttcc gtaaaccgcg
    15361 catagccgcc cttctcccaa cgttcactca actcggcaaa aacatcaaaa tcattacgcg
    15421 cttcatagcg tggcggcacc acttgcttca tcggcaccag atgctgatta ctgtaatcac
    15481 cggtcatggt gagatcatta cgctcaaaag aggtagtcgc aggcagaacg atatccgcgt
    15541 gtttcgccgc cgccgtccag aagcattcag agatcaccac cagctccggt ttttgccagg
    15601 cacggatcag acgattggta tcctgatgat gagtaaagtt ggcaccgccc gcccaccaga
    15661 taaaacgaat atccgggaaa tgtcggttca taccgttgtg ttgatatgcg ccaccagggt
    15721 tttccagtgc ttcaacaatg cgggcaacag ggattttatc caccgcatcg cagccacccg
    15781 gcaagctgcc ctgcatggaa gagagcaccg cagaacgccg cgtggggtta ccaccattgg
    15841 caaaatggta agaaagacca aaaccaccgc cgggtgtgcc gatttgcccc aacattgctg
    15901 ccagcgtgac gatcatccag tgtttttgct ccccaaactg ttggcgctgc attccccagc
    15961 ctgccatcag catggtggta ttttggtgga aaatagccgc cagctcgcgg attttcgctg
    16021 cgccaacacc acaaatctct gctgcccatt cggcggtttt cgctattccg tcactctcgc
    16081 ccagcaaata agaggcgaag acggcataac ctgtggtgca acgcgccaga aacgcttcgt
    16141 cgtgccaacc attttccacc agcgtatggg cgatccccag catcagcgca acatcggtgc
    16201 ccatgtgcgg tgccacccac tccattttat cgccaaagaa atcgacggtt tccgatcgca
    16261 ttggatcaat gcagatcagc tttttcccac tgtcacgcag tgcagaaaag taagaaagcc
    16321 cctgctcatc ggatgcattc cacgcaattt tcagcgtatt gagtggatta gcactccaca
    16381 gcaccacgac atcgctatgt tccagcacca gcggccaact ggtttgctgt tgataaactt
    16441 cgctgccacc tacgacatac ggcatgatcg cctgtgccgc accagtcgaa taatccccca
    16501 gatgcccggt ataaccgcct gccagcgcca tatagcgttg taataatgtc gaggccttat
    16561 gcagcacgcc gtttgaacgc cagccgtagg aaccagcaaa aatcgatgcc ggaccataag
    16621 cctcacgaat gcgtttatgt tgtaggtgaa taagatccag cgcctcatcc caactcacgc
    16681 gaacaaattc atcctgccca cgaatgcctt gcgggttttc cggtgacgcg agaaagcctt
    16741 tgcgcaccat tggaaatcgt acccgcgtat tgctgtgaac ctggtcgcga accgcgctct
    16801 gcaaggagtt ttccattcct gtggctaacg cgccacgcga gctaaacacg gtttcgccgt
    16861 cggtttcaac cagcatgggc ccccagtggg cggcagtcag aacggaatat cgtgaggatg
    16921 agttggccaa accgaagact cctgaaaggt tagatgtatt gagcagttaa atattactta
    16981 caaatttccg agtattccca ggaataatct tcatgttcac gcggcataat ctcccgccac
    17041 ggaacccgtg gcaaagaata aaaaggttat taaggattaa caatgaagaa acgtgtttat
    17101 cttattgccg ccgtagtgag tggcgctctg gcggtatctg gctgcacaac taacccttac
    17161 accggcgaac gcgaagcagg caaatctgct atcggcgcag gtctgggctc tctcgtgggc
    17221 gcgggtattg gtgcgctctc ttcttcgaag aaagatcgcg gtaaaggcgc gctgattggc
    17281 gcagcagcag gcgcagctct gggcggcggc gtgggttatt acatggatgt gcaggaagcg
    17341 aagctgcgtg acaaaatgcg cggcactggt gttagcgtaa cccgcagcgg ggataacatt
    17401 atcctcaata tgccgaacaa tgtgaccttc gacagcagca gcgcgaccct gaaaccggca
    17461 ggcgctaaca ccctgaccgg cgtggcaatg gtgctgaaag agtatccgaa aacggcggtt
    17521 aacgtgattg gttataccga cagcacgggt ggtcacgacc tgaacatgcg tctctcccag
    17581 caacgtgcgg attccgttgc cagcgcgttg atcacccagg gcgtggacgc cagccgcatc
    17641 cgtactcagg gccttggccc ggctaaccca atcgccagca acagcaccgc agaaggtaag
    17701 gcgcaaaacc gccgtgtaga aattacctta agcccgctgt aatccctttc atgccaggtg
    17761 atgcagaaat cacctggcat tcttcagttt tccttcatca tatttcaggc taaggtgatc
    17821 gccttatcag tgaatggaga gaagcatgaa gccgtccgtt atcctctaca aagccttacc
    17881 tgatgattta ctgcaacgcc tgcaagagca tttcaccgtt caccaggtgg caaacctcag
    17941 cccacaaacc gtcgaacaaa atgcagcaat ttttgccgaa gctgaaggtt tactgggttc
    18001 aaacgagaat gtagatgccg cattgctgga aaaaatgccg aaactgcgtg ccacatcaac
    18061 gatctccgtc ggctatgaca attttgatgt cgatgcgctt accgcccgaa aaattctgct
    18121 gatgcacacg ccaaccgtat taacagaaac tgtcgccgat acgctgatgg cgctggtgtt
    18181 gtctaccgct cgtcgggttg tggaggtagc agaacgggta aaagcaggcg aatggaccgc
    18241 gagcataggc ccggactggt acggcactga cgttcaccat aaaacactgg gcattgtcgg
    18301 gatgggacgg atcggtatgg cgctggcaca acgtgcgcac tttggcttca acatgcccat
    18361 cctctataac gcgcgccgcc accataaaga agcagaagaa cgcttcaacg cccgctactg
    18421 cgatttggat actctgttac aagagtcaga tttcgtttgc ctgatcctgc cgttaactga
    18481 tgagacgcat catctgtttg gcgcagaaca attcgccaaa atgaaatcct ccgccatttt
    18541 cattaatgcc ggacgtggcc cggtggttga tgaaaatgca ctgattgctg cattgcagaa
    18601 aggcgaaatt cacgccgccg ggctggatgt cttcgaacaa gagccactgt ccgtagattc
    18661 gccgttgctc tcaatggcca acgtcgtcgc agtaccgcat attggatctg ccacccatga
    18721 gacgcgttat ggcatggccg cctgtgccgt ggataatttg attgatgcgt tacaaggaaa
    18781 ggttgagaag aactgtgtga atccgcacgt cgcggactaa gccgcgactg cgtgaagtaa
    18841 agcccgataa tcgctcgggc ttttactctt tattgggttg cagtaactgc tgtagtccag
    18901 gcctgattaa acgcctgatg ttgtgccggt aacggcgcaa tcagtttgtt atattcactt
    18961 gcctgctgcg aagtcgggaa ctggatacca tttgctacaa agctcacctg agtaccttgt
    19021 tgagcaatgt agtctccaac catcaccagt tgttgcgtga aagtttgtgc agccggaatc
    19081 aacggttgca atgcatcagc tggcgtcgtt accactttgg tgaatgcctg atcaaagacc
    19141 ggtttcaaat catcggtctg ttttagcgca gagtgcgcag catcggcctg caacttcgca
    19201 ttttgcagtt gttgcgccag cacacccagc gaaccgttca tttcacgcag cggaccgctt
    19261 tgcgtaacgt aatcctgcgg tacgcgaatt gcgttaacgc tgtctacgac cgggcgcaga
    19321 ccggaatcca tcgcctgatt aacctgctga gaataaccat acagaatcgc gtaatcagag
    19381 acaaaaggac caaactgttt tttctgatcg gcagtcaggg ttggtagacg ttcaccgcta
    19441 cgcatcactg tattctgcag gaagtcgata aacgctttac gctgatcgcc ttctttatca
    19501 aaacacccac tcaggctaac taccattaat aacgccgcaa gaggcgcaaa ccagcgagag
    19561 caggactttc ctgtcgccat tttcttactc ctttcaccca aaaaaagcgc acaacgacac
    19621 acgcgtgtct gacgtgacaa ggatagtcca ggacaggctt gcaggatacc ctttctacgt
    19681 aatcttcctg gtaaaatcat ggagaaaatg aaacaaggtt aatttgttgt ttcattgtta
    19741 aaaaatgata gtgccgttgc tttttttgta gccagagatt tacgccttcg cagaaggtta
    19801 gattttcgaa atctctacaa accaggagtg gtaagagata tatcggcatt tatcagatcg
    19861 ttaggtggct ataagtcacg ctaaatgact ggctgaatcg aatcatagcc agagcatgcc
    19921 ctgacttcac cccgctgtgt ctgcttttcc cgactattct taatgagctt cgatgcaatt
    19981 cacgatcccg cagtgtgatt tgaggagttt tcaatggaat ataaagatcc aatgcatgag
    20041 ctgttgagca gcctggaaca gattgttttt aaagatgaaa cgcagaaaat taccctgacg
    20101 cacagaacaa cgtcctgtac cgaaattgag cagttacgaa aagggacagg attaaaaatc
    20161 gatgatttcg cccgggtttt gggcgtatca gtcgccatgg taaaggaatg ggaatccaga
    20221 cgcgtgaagc cttcaagtgc cgaactaaaa ttgatgcgtt tgattcaagc caacccggca
    20281 ttaagtaagc agttgatgga atagactttt atccacttta ttgctgttta cggtcctgat
    20341 gacaggaccg ttttccaacc gattaatcat aaatatgaaa aataattgtt gcatcacccg
    20401 ccaatgcgtg gcttaatgca catcaacggt ttgacgtaca gaccattaaa gcagtgtagt
    20461 aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt cctttaacgc
    20521 ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt aaaggtaata
    20581 cactatgtcc ggtaaaatga ctggtatcgt aaaatggttc aacgctgaca aaggcttcgg
    20641 cttcatcact cctgacgatg gctctaaaga cgtgttcgta cacttctctg ctatccagaa
    20701 cgatggttac aaatctctgg acgaaggtca gaaagtgtcc ttcaccatcg aaagcggcgc
    20761 taaaggcccg gcagctggta acgtaaccag cctgtaatct ctgcttaaaa gcacagaatc
    20821 taagatccct gccatttggc ggggattttt ttatttgttt ttcaggaaat aaataatcga
    20881 tcgcgtaata aaatctagta ttatttttgt gaaaaataaa tttgggtgca atgagaatgc
    20941 gcaacgccgt aagaaaggcg ggaataactt cccgccgaag actcttactc ttttaaattg
    21001 caggctaaaa atgccgccag ctcataactc ccctgtttaa tatgcaattc acacagtgaa
    21061 tctcttatca tccaggtgaa aaataaaagc gtgaaacaaa tcactattaa agaaagtaat
    21121 ctatatttct gcggcattcc cagccccctg tgttgatttc caacgagtaa gaggctaacc
    21181 ttatggtgtt caggcatagg gtagcctcac tttgatttat agtcaggtgg ggcttttctc
    21241 tgtctgcctt tcggtgaata cctgagacaa acagtctcaa gcacccgtgg ctattctagc
    21301 ttaataagtt tgtttcttct ccttgatata atcctaaaaa aacctcataa aattaatata
    21361 tgagataatc tttattcagc agaagattat taaaggttgc tgtattattt agcgataata
    21421 aaaaaagcct gccagatggc aggcttttta ataacggcgt tattactgca acagcgaaat
    21481 atccgcaacg cgcaggaaca gttcgcgcag tttctccagc atggtcagac ggttgatacg
    21541 caattctttg tcatcaacca ttaccatcac tttatcgaag aaagcatcaa ccggttcacg
    21601 cagctcagcc agttcgacca gcgcatcctg gtaacgacct tccgtaaagt acggctccag
    21661 cttgtcacgt agcaccacaa cctgcatcgc cagtttaatt tcttccggct ctttcagagt
    21721 agaggcattc acgcgatcgc tcagcacttc gtcagatttc gccaaaatgt tggagacacg
    21781 cttgttcgcc gccgccagtg cagcagctgc ttcaagggta cggaagtgcg ataccgcttt
    21841 catgcgggca tcgaaatcag ccggacgagt cggacgacgc gccagtaccg cctggatggt
    21901 gtcaacggta taaccttcgt cctgatacca ggcgcggaag cgaccgagca taaagtcgat
    21961 aacatcatca actacgttgg cattagtcag cttatcgcca tacagacgca ccgcttcttc
    22021 ggtcagcgtt tgcagatcaa ggttgaggtt cttctcaacg ataattcgca gcacgccaag
    22081 tgcggcacga cgcagcgcaa acgggtcttt gtcgcctttc ggatgctggc cgataccgaa
    22141 gatacccgcc agagtatcca tcttgtcagc aatcgccagc gcacaggcta ccgggttaga
    22201 cggcaggtca tcaccggcaa agcgcggctg atactgctca ttcagcgcca ccgcgacatc
    22261 ttccgcttcg ccatcgtgac gcgcatagtg catccccata acgccctggg tgtcggtgaa
    22321 ctcgaagacc atgttggtca tcaggtcgca cttagacagc agacccgcac gggtagcgtg
    22381 gttaacgtca gcgccaatct gttcagcaat ccagccagcc agcgcctgga tgcggtcagt
    22441 tttgtcgcgc agcgtcccca actgttgctg gaacaacacg gtttgcaggc gcggcaggtt
    22501 atcttcaaga cgttttttac ggtcggtgtt gaagaagaac tcggcatccg ccagacgcgg
    22561 acgaacgact ttctcgttac cagagataat ttgctgcgga tctttcgatt cgatgttggc
    22621 aacgaagata aagttcggca gcagtttgcc gtcgttcgca tacaccggga agtatttctg
    22681 gtcacctttc atggtgtaaa ccagcgcttc agccggcacc gcgaggaatt tctcttcgaa
    22741 ttttgcggtc agaacgaccg gccactccac cagcgaagcc acttcttcca gcaggctttc
    22801 gcttaagtca gcgttaccgc caatcttacg cgctgcttct tcggcatcgg ctttaatctt
    22861 cgccttacgt tcttcgtaat cggcgatgac tttcccacgc tcacgcagaa tttccggata
    22921 ctgatcggcg ttatcgatgg tgaattccgg ctcgcccata aagcggtggc cgcgaatcac
    22981 gcgatcggac tgaatgccca gaatggttgc cggaatgact ttgtcgccca gcagcagggt
    23041 cacggtgtgc accggacgca cgaagtgcac gtcgcttgcg ccccaacgca tcagtttcgg
    23101 gatcggcagt ttcgccagag aagtcgcaac catattcggc agcagtgctt cggtgctttc
    23161 gcccttcaca tgggcgcgat acagcagcca ttcgccttta tcggtagtca gacgctcagc
    23221 ctggtcaacg gtaataccgc aaccacgcgc ccaaccttct gccgctttgc tcggtttgcc
    23281 ttcagcgtcg aacgcctggg caatcgccgg gccgcgtttt tcgatttcac gatccggttg
    23341 cgcttccgcc aggttagcta ctttcagcgc cagacgacgc ggagcagcaa accattgaac
    23401 ggtgccgtgt gcgaggccag cgttatccag ctccgcagta aaattcgcag caaaggactc
    23461 agccaggctg cgcagtgctt ttggtggcag ctcttcagtg ccgatttcca ccagaaaagt
    23521 tttctcagac atagccgcct cttacttatc tttgttgcac atcgggaagc cgagggcttc
    23581 acgggaagcg tagtatgctt ctgccactgc tttggtcagg gtgcgaatgc gcagaatgta
    23641 gcgctgacgc tcggtgacgg agatggcttt acgcgcatcc agcaggttga agctgtgggc
    23701 ggctttcaga atacgctcgt aggctggcag cggcagcgga ttttccagcg ccagcagctg
    23761 ctgcgcttct ttctcgtact gctcaaagca ggtgaacagg aagtccacat ccgcgtattc
    23821 gaagttgtaa gtggactgct ccacttcgtt ctgatggaac acgtcgccgt aggtggtttt
    23881 acccagcggg ccgtcgctcc agaccaggtc gtaaacgctg tctacgccct gaatgtacat
    23941 ggccagacgt tccagaccgt aggtgatctc gccggtaacc ggtttacact ccagaccacc
    24001 aacctgctgg aagtaagtga actgcgtcac ttccatgccg tttagccaca cttcccagcc
    24061 cagtccccag gcacccagag tcgggttttc ccagttatct tccacgaaac ggatgtcgtg
    24121 aatagtcggg tccatgccca gctctttcag agaaccgagg tacagctcct gaatattgtc
    24181 cggcgatggc ttaatgacca cctggaactg atagtagtgc tgtaaacggt tggggttttc
    24241 gccgtagcga ccatcggtcg ggcgacgaga aggctgaaca taagcagccg ccatcggttc
    24301 tggccccagc gcgcgcagac aggtcattgg gtgagaggtt cccgcgccga cttccatgtc
    24361 caatggttga acaatggtgc agccctggcg agcccagtaa tcctgtaagg tcaggatcaa
    24421 gccctggaag gtcctggtat caaacttttg catattattt cgtgctggat acgtgtggat
    24481 ttaaaggaag ggatcagtat acccgctgga tggaagatat acagtacgaa acgggaaaaa
    24541 gcagggctta acgcatggaa agatgcaaaa aatggccatc tgcgtcaaaa gaacagacaa
    24601 actgctcttt acggaaggta taaccgcgca tttcatagct tccctggaac tgttcgaagg
    24661 cggtgacgtc gattttttgc tcgccggtgt tataacgttt tgccgcctga tccttgcata
    24721 acgcttccat attcaacgaa cgcagaggat caacctttac ccgttgtgct ttctgcacgg
    24781 gtgacggtgt agaacaaccc actagcacca taagcgccat tgccggaaag aaagcgttca
    24841 tcatcattaa attaccgtct ggtttgcagg tgagtcttat tatttatatc ggtatagtgg
    24901 ctgatatctt acgaatttcc ttaagcctct ataagctgta gtgataaaac tcagattatt
    24961 ccagtgcgcc aaccaaatga tttatcggtc agactttttt taaccagata cagagacaca
    25021 gatgcagccc aaaatttact ggattgataa cctgcgaggg atagcgtgtt taatggtggt
    25081 gatgattcac accactacct ggtatgtgac caatgctcat agtgttagcc ccgtcacctg
    25141 ggatatcgcc aatgttctga attctgcctc tcgtgtcagc gtgccgctat ttttcatgat
    25201 atccggctat ctcttttttg gcgaacgcag cgcccagccg cgccatttct tgcgtatcgg
    25261 cttatgtctg attttttata gcgcaatcgc actgctctac attgcgctgt ttacctccat
    25321 caatatggag ttagcgctga aaaacctgct gcaaaagcca gtgttttacc acttgtggtt
    25381 tttcttcgcg attgcggtga tttatctggt ttcaccgctg attcaggtga agaacgtcgg
    25441 cggaaaaatg ttgctggtgc taatggcggt gattggcatt atcgctaacc caaacacagt
    25501 gccgcagaaa attgacggtt ttgaatggct gccaattaac ttatatatca atggcgatac
    25561 tttttactac attctgtatg gcatgttggg ccgcgctata gggatgatgg acacacagca
    25621 taaagcactg tcgtgggtga gcgccgcgct gtttgcgacg ggggttttta ttatctctcg
    25681 cgggacatta tatgaattgc agtggcgcgg aaattttgcc gatacctggt atctttactg
    25741 tgggccgatg gtttttatct gcgcaatcgc gctattgact ctggttaaaa acacgctgga
    25801 tacgcgtacc attcgcggac ttggcttaat ctcccgccat tcattgggta tatacggatt
    25861 ccacgccttg attatccatg cgctgcgcac ccggggaatt gagcttaaaa attggccaat
    25921 actggatatt atttggatct tttgcgcgac gttggcagcg agtttgttac tttctatgct
    25981 ggtacaacga atcgacagaa acagattagt gagttaagta aaagcccgtt cacattggac
    26041 tgaccgggct tacgtgaatt attcgctgta ttcttcctgg gtaacctgtg tttcttttgg
    26101 cgggtttatt ccggcatcac gaatattctt ctgcaccgct actgcaccaa acaagcttaa
    26161 gaagaaagcc agtccataaa aacctttttc actgagtaat aatgtcgcat tccacagacc
    26221 taccatcagt aatgcaacag agataataaa gacagtcagg caggtcatat aataaatgga
    26281 agtggtcggt atgccttcat acttgtcccg aacggtcttt tgataagacg cagcagaaaa
    26341 cagtcctaac accagtacgg caaaataata ccctttttca tttaactgca tctctgcatt
    26401 ccatagccct aacagatagg taacgatacc accaacgaga gctatccatg acacaatact
    26461 aaaggccggt gaataggttg atattttgtt gtccatcaca gtattccttt cattcctgaa
    26521 tgtgtaagag ctttatgttg ctcataccga gcgaaacaaa cgcatttgac caaacaagca
    26581 gacaaaaaag gcgatggggt agatcaccat ttcataagca ttgataggtg catttaaaac
    26641 accgataaac aagagaccga tagtgattaa cgcaactaac tggcagatat gggtaaaaaa
    26701 attatccagt tgcccgagtt tttctgcgcg aagatatgca tagttaccaa aagttgctgt
    26761 cattaacacg ccaagaaaat agccttttcc acttagcaat gggcatgaga tccatagccc
    26821 aaccagataa accagcgccc cgacaacaaa taatagtttt gccacagttt ttgaggtttt
    26881 cataacgatc tccatatcta ccagcgatac attacgagta accaacgaaa gacaaaactg
    26941 aaaaatgcca ttaacaaatg attttcagaa taaattcata ctaaatatta attaattagt
    27001 gagatatata gatgtgaatt atcccccacc cggtcaggca ggggataacg tttacgccat
    27061 taatggcaga agttgctgat agaggcgacg gaacgtttca cgtcgtggct gataagcggc
    27121 ataacgctgc gcatctggta gatgcgactg ttctaacggt agctgcggca acaactcaat
    27181 gagcgatttc tctggattcg ccgcaatctg cgccagcctt gctgcgccca gtgctggccc
    27241 cacatccccc cccgtacggt aatcgagctg ctgaccgctg atatccgcca gcatctgacg
    27301 ccagtactca ctacgcgccc cgccaccaat caacgtaaca ctttgcggtt taatgccgca
    27361 ggcatgcacg acatccatgc catctgccag cgcataaccc acgccttcca gcactgctcg
    27421 cgccagttca ttggggccat gttgatgagt caaaccaaag aaaaccccct tcgcctgggg
    27481 attattgtgt ggtgtacgct cgccggaaag ataaggcaga aaccaaactg gctcggcact
    27541 ttcatcagcc tgttgagctg cagcgattaa agctgggaca ttgcacaggc cggttaattt
    27601 cgcgacccaa tccagacacg atgctgcact cagcattaca gacattaaat gccaacgctg
    27661 cggtagcgca tggcaaaagc tgtgtacagc gctttctggc ttgcttaaga acccttcgct
    27721 gacagcaaaa tagacccccg acgtccccag cgataacatt gcctgattag catcaaccat
    27781 tcccacacca actgcaccag ctgcattgtc gccaccgcct gcgacaactg gcaccgtcgc
    27841 cataccccac gctttcgcaa cttcaggtaa caaagcacca gtaatttcgc tgccttcgta
    27901 taatgcgggc atctggtcac gagataagtc gcaagcctgc agcatgacgt cactccagtc
    27961 acgctttgcg acatccagcc acatggtgcc agctgcgtca gacatatcgc tggcaaactc
    28021 ccccgtcata cgcagacgca agtaatcttt cggtaataat actttgtcga tttgacggaa
    28081 tatctccggc tcatgccgct gaacccatag caatttaggc gcagtaaatc cgggcatcat
    28141 caggttgccg gtaatcactc gtgattgcgg aactctcgct tccagcaaag tgcactcttg
    28201 cgcacagcgc ccgtcgttcc acaaaatggc agggcgcaat acccgttgtt gagcatccag
    28261 taaggttgct ccatgcatct ggccggcaat acccaatgct ttaacgtcct gcagagaatg
    28321 ctgatcgccc agagctttca ttgcgcgatc agttgcctgc caccactgtt ccgggtcttg
    28381 ttccgaccag agtggatgcg ggcgcgaaac ggtcagcttt tccgtttgcg aagcaaccac
    28441 ctcaccctgc tcgttgagca aaataacttt tacgcccgag gtgccaagat ctatcccgat
    28501 atacatatcg atcgttcctt aaaaaaatgc ccggtatcgc taccgataac cgggccaacg
    28561 gactgcacag ttagccgtta tttgtcgaac agataatgat ttaccagatt ttccagttgc
    28621 tcctggcgac cactctgatg caccggagac aaattatgtt cctgagcata tttggctaaa
    28681 tctgccagtg acatttggcc tttcaggatt tgctggccca attcgctatt ccagccggaa
    28741 taacgctgcg cgatgcgttt atccagctcg ccatcttcaa tcatgcgcgc tgcaattttc
    28801 agcgccagtg ccatcgtatc catcgcgccg atatgaccgt aaaacagatc atatttatca
    28861 gtactttgac gacgtacttt ggcatcgaag ttcaaaccac cggtggtgaa accgcctgct
    28921 ttgagaattt catacatcac cagcgcattc tcttccacac tgttcgggaa ctggtcggtg
    28981 tcccagccca gttgcgcatc gccacggttg gcgtcgacag aaccgaacag gccaagcgca
    29041 atggcggtgg ctatttcatg atggaaagag tgacctgcca gcgtcgcgtg gttagcttca
    29101 atgttcagtt taatctcttt ttccagacca aactgtttca ggaagccata gaccgtcgcg
    29161 gcatcgtaat catattgatg tttagtcggt tcttgcggtt tcggttcgat aagcaacgtg
    29221 ccctggaagc cgattttatg tttatgctca accaccatct gcataaagcg gcccagttgt
    29281 tcacgctcct gacgcaagtc ggtatttaac agcgtttcgt aaccttcacg accaccccac
    29341 aggacatagt tttcaccgcc caatttatgg gttgcttcca tcgctgtaac aacttgcgtt
    29401 gccgcccagc tgaagacttc aggatctggg ttcgtcgccg cacccgcgcc gtagcgaggg
    29461 tttgtaaagc agttggcggt tccccacagc agcttcacgc cgctctcttc ttgcttgcct
    29521 gccaggacat caaccatttg cgcaaaatta ttgatgtact cttttaacga cgcgccctca
    29581 ggagaaacat ccacatcgtg gaagcaataa aatggcacat gtaacttgtg gaaaaactca
    29641 aatgcgacat ctgctttacg cttcgccaac gccagtgcct caccaggctg ctgccacgga
    29701 cgattaaacg cccccacacc aaacatgtcc gccccgttcc agcagaaggt gtgccagtag
    29761 caggcggcaa atcgcaagtg ctcttccata cgcttaccca acaccagttc gtcgggattg
    29821 tagtgacgga atgctaacgg gtttgaggat tttgagcctt cataacgaac gcgatcgagc
    29881 tggtcaaaat aggcttgcat attgaactcc ataatcaggt aatgccgcgg gtgatggatg
    29941 atgtcgtaat attgggcact ccctttcagt tgctcaatta tgttatttca cactgctatt
    30001 gagataattc acaagtgtgc gctcgctcgc aaaataaaat ggaatgatga aactgggtaa
    30061 ttcctcgaag agaaaaatgc aataagtaca attgcgcaac aaaagtaaga tctcggtcat
    30121 aaatcaagaa ataaaccaaa aatcgtaatc gaaagataaa aatctgtaat tgttttcccc
    30181 tgtttagttg ctaaaaattg gttacgttta tcgcggtgat tgttacttat taaagctgtc
    30241 ctctaactac agaaggccct acaccatgaa aataaagaac attctactca ccctttgcac
    30301 ctcactcctg cttaccaacg ttgctgcaca cgccaaagaa gtcaaaatag gtatggcgat
    30361 tgatgatctc cgtcttgaac gctggcaaaa agatcgagat atctttgtga aaaaggcaga
    30421 atctctcggc gcgaaagtat ttgtacagtc tgcaaatggc aatgaagaaa cacaaatgtc
    30481 gcagattgaa aacatgataa accggggtgt cgatgttctt gtcattattc cgtataacgg
    30541 tcaggtatta agtaacgttg taaaagaagc caaacaagaa ggcattaaag tattagctta
    30601 cgaccgtatg gttaacgatg cggatatcga tttttatatt tctttcgata acgaaaaagt
    30661 cggtgaactg caggcaaaag ccctggtcga tattgttccg caaggtaatt acttcctgat
    30721 gggcggctcg ccggtagata acaacgccaa gctgttccgc gccggacaaa tgaaagtgtt
    30781 aaaaccttac gttgattccg gaaaaattaa agtcgttggt gaccaatggg ttgatggctg
    30841 gttaccggaa aacgcattga aaattatgga aaacgcgcta accgccaata ataacaaaat
    30901 tgatgctgta gttgcctcaa acgatgccac cgcaggtggg gcaattcagg cattaagcgc
    30961 gcaaggttta tcagggaaag tagcaatttc cggccaggat gcggatctcg caggtattaa
    31021 acgtattgct gccggtacgc aaactatgac ggtgtataaa cctattacat tgctggcaaa
    31081 tactgccgca gaaattgccg ttgaattggg caatggtcag gagccaaaag cagatacctc
    31141 actgaataat ggcctgaaag atgtcccctc ccgcctcctg acaccgatcg atgtgaataa
    31201 aaacaacatc aaagatacgg taattaaaga cggattccac aaagagagcg agctgtaagc
    31261 gttacgcccc ggcgcggagc gggggcgtga tttctctcca tgccgcgtga atgaattggc
    31321 ttaggtggag tcgttatgcc ttatctactt gaaatgaaga acattaccaa aaccttcggc
    31381 agtgtgaagg cgattgataa cgtcagcttg cggttgaatg ctggcgaaat cgtctcactc
    31441 tgtggggaaa atgggtctgg taaatcaacg ctgatgaaag tgctgtgtgg tatttatccc
    31501 catggctcct acgaaggcga aattattttt gcgggagaag agattcaggc gagtcacatc
    31561 cgcgataccg aacgcaaagg tatcgccatc attcaccagg aattggccct ggtgaaagaa
    31621 ttgaccgtac tggaaaatat cttcctgggt aacgaaataa accataatgg cattatggat
    31681 tatgacctga tgacgctacg ctgtcagaag ctgctcgcac aggtcagttt atccatttca
    31741 ccagataccc gcgtgggaga tttagggctt gggcaacaac aactggttga aattgccaag
    31801 gcacttaata aacaggtgcg cttgttaatt ctcgatgaac cgacagcctc attaactgag
    31861 caggaaacgt cggttttact ggatattatt cgcgatctac aacagcacgg tatcgcctgt
    31921 atttatattt cgcacaaact caacgaagtc aaagcgattt ccgatacgat ttgcgttatt
    31981 cgcaacggtc agcacattgg tacgcgtgat gctgccggaa tgagtgaaga cgatattatc
    32041 accatgatgg tcgggcgaga gttaaccgcg ctttacccta atgaaccaca taccaccgga
    32101 gatgaaatat tacgtattga acatctgacg gcatggcatc cggttaatcg tcatattaaa
    32161 cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa tactgggtat tgccggactc
    32221 gttggtgccg gacgtaccga gactattcag tgcctgtttg gtgtgtggcc cggacaatgg
    32281 gaaggaaaaa tttatattga tggcaaacag gtagatattc gtaactgtca gcaagccatc
    32341 gcccagggga ttgcgatggt acccgaagac agaaagcgcg acggcattgt tccggtaatg
    32401 gcggttggta aaaatattac cctcgccgca ctcaataaat ttaccggtgg cattagccag
    32461 ctcgatgacg cggcagagca aaaatgtatt ctggaatcaa tccagcaact caaagttaaa
    32521 acgtcgtccc ccgaccttgc tattggacgt ttgagcggcg gcaatcagca aaaagcgatc
    32581 ctcgcccgct gtctgttact taacccgcgc atcctcattc ttgatgaacc taccaggggt
    32641 attgatattg gcgcgaaata tgagatctac aaattaatta accaactcgt ccagcagggt
    32701 attgccgtta ttgtcatctc ttccgaatta cctgaagtgc tcggccttag cgatcgtgta
    32761 ctggtgatgc atgaagggaa actaaaagcc aacctgataa atcataacct gactcaggag
    32821 caggtgatgg aagccgcatt gaggagcgaa catcatgtcg aaaagcaatc cgtctgaagt
    32881 gaaattggcc gtaccgacat ccggtggctt ttctgggctg aaatcactga atttgcaggt
    32941 cttcgtgatg attgcagcta tcatcgcaat catgctgttc tttacctgga ccaccgatgg
    33001 tgcctactta agcgcccgta acgtctccaa cctgttacgc cagaccgcga ttaccggcat
    33061 cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa atcgaccttt ccgtcggctc
    33121 aatgatgggg ctattaggtg gcgtcgcggc gatttgtgac gtctggttag gctggccttt
    33181 gccacttacc atcattgtga cgttggttct gggactgctt ctcggtgcct ggaacggatg
    33241 gtgggtcgcg taccgcaaag tcccttcatt tattgtcacc ctcgcgggca tgttggcatt
    33301 tcgcggcata ctcattggca tcaccaacgg cacgactgta tcccccacca gcgccgcgat
    33361 gtcacaaatt gggcaaagct atctccccgc cagtaccggc ttcatcattg gcgcgcttgg
    33421 cttaatggct tttgttggtt ggcaatggcg cggaagaatg cgccgtcagg ctttgggttt
    33481 acagtctccg gcctctaccg cagtagtcgg tcgccaggct ttaaccgcta tcatcgtatt
    33541 aggcgcaatc tggctgttga atgattatcg tggcgttccc actcctgttc tgctgctgac
    33601 gttgctgtta ctcggcggaa tgtttatggc aacgcggacg gcatttggac gacgcattta
    33661 tgccatcggc ggcaatctgg aagcagcacg tctctccggg attaacgttg aacgcaccaa
    33721 acttgccgtg ttcgcgatta acggattaat ggtagccatc gccggattaa tcctcagttc
    33781 tcgacttggc gctggttcac cttctgcggg aaatatcgcc gaactggacg caattgcagc
    33841 atgcgtgatt ggcggcacca gtctggcagg cggcgtcggg agcgtggctg gagcggtaat
    33901 gggggcattc atcatggctt cactggataa cggtatgagt atgatggatg taccgacctt
    33961 ctggcagtat atcgttaaag gtgcgattct gttgctggca gtatggatgg actccgcaac
    34021 caaacgccgt tcttgatttt gataaaaatt ttctcaaagc cggtaacgta ttaccggttt
    34081 tgagtttttg catgattcag caggaaaaga accatgttta ctaaacgtca ccgcatcaca
    34141 ttactgttca atgccaataa agcctatgac cggcaggtag tagaaggcgt aggggaatat
    34201 ttacaggcgt cacaatcgga atgggatatt ttcattgaag aagatttccg cgcccgcatt
    34261 gataaaatca aggactggtt aggagatggc gtcattgccg acttcgacga caaacagatc
    34321 gagcaagcgc tggctgatgt cgacgtcccc attgttgggg ttggtggctc gtatcatctt
    34381 gccgaaagtt acccacccgt tcattacatt gccaccgata actatgcgct ggttgaaagc
    34441 gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg ctttttatgg tcttccggaa
    34501 tcaagcggta aacgttgggc cactgaacgc gaatatgcat ttcgtcagct tgtcgccgaa
    34561 gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg cgccagagaa ctggcaacac
    34621 gcgcaaaatc ggctggcaga ctggctacaa acgctgccac cgcaaaccgg gattattgcc
    34681 gttactgacg cccgggcgcg gcatattctg caagtatgtg aacatctaca cattcccgta
    34741 ccggaaaaat tatgcgtgat tggcatcgat aacgaagaac tgacccgcta tctgtcgcgt
    34801 gtcgcccttt cttcggtcgc tcagggcgca cggcaaatgg gctatcaggc ggcaaaactg
    34861 ttgcatcgat tattagataa agaagaaatg ccgctacagc ggattttggt cccgccagtt
    34921 cgcgtcatcg aacggcgctc aacagattac cgctcgctga ccgatcccgc tgttattcag
    34981 gccatgcatt acattcgtaa tcacgcctgt aaagggatta aagtggatca ggtactcgat
    35041 gcggtcggga tctcgcgctc caatcttgag aagcgtttta aagaagaggt gggtgaaacc
    35101 atccatgcca tgattcatgc cgagaagctg gagaaagcgc gcagtctgct gatttcaacc
    35161 accttgtcga tcaatgagat atcgcaaatg tgcggttatc catcgctgca atatttctac
    35221 tctgttttta aaaaagcata tgacacgacg ccaaaagagt atcgcgatgt aaatagcgag
    35281 gtcatgttgt agcgatgatg agaattgtcg gcgtcgcatc aggtaactca gcacaaccac
    35341 ctgatgcgag caaaagcctt atccgactga caagtcggat aaggctcgag gaaaagcaat
    35401 tacatatgcg ccgcgattaa ccgttggtta tcctggtaca ttgcgaacag gtagttgtta
    35461 taactcttcc ctttggtcga gtagcccttc agcttgtgaa tcatcgctgt ggcagtcact
    35521 tcctgatccg ctttacgcag ctgcgcacgc gatttacgga acgaagagta agccgggtgc
    35581 gtattcaggt tagtgacata ggcgctcacc gattctttga cagaactaaa ctgtgagtac
    35641 cccttcactt tacccggcgc attagtacaa cgtcctttca tgcatttcat gccgaacaga
    35701 ttgttgttgt tgcgcgccag ctttgacgtt ccccaaccgc tttctgctgc agccatcgtc
    35761 gccaccatac tggtggggat aatgtctacg cgttcaagca aggtattcca cgggattttt
    35821 cgcgtattac cagaccactt caccttgtag cgtttggcga tgtctttcag acgcgcacgc
    35881 tcagcaggtg accattggcc ctgatactgt tttgaaatca gccagttacg ttccgcagta
    35941 atggctgcat tttggctggt aatgtaaggc attacggtcc ggagaaacgc ctttttcctt
    36001 ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac tactctttgc actattgcga
    36061 gaatactctt gtttactgct tacctgttta ttactagctt tagttaagtg ggacttttga
    36121 ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg aaaacaccag agtgagtaac
    36181 ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa tcatcaggtc tcctggtcgg
    36241 atttaatcat tccaacacct tatatttttc acaaatttga gagttgaatc tcaaatcata
    36301 tcaaaaatag ctgtcaagag caccccaagg aatagtccaa atctgaaact atgtcacgag
    36361 ttaacgattc agattggcgc taaatcgcag aaaatgtggg gtttatcgca aaattcagcc
    36421 gttttttgcg cgcgatcgct cacccttgct tctcatcctg tggagttacc gctcagggat
    36481 gagttttgtc tggcttatcg ctggcaaact gtctgaaatc gcagcaataa ggactcatcc
    36541 gccatgaaac tcgccgccta ttttctgaca ctccttcctg gcttcgccgt tgccgccagc
    36601 tggacttctc cggggtttcc cgcctttagc gaacagggga cgggaacatt tgtcagccac
    36661 gcgcagttgc ccaaaggtac gcgtccacta acgctaaatt ttgaccaaca gtgctggcag
    36721 cctgcggatg cgataaaact caatcagatg ctttccctgc aaccttgtag caacacgcca
    36781 cctcaatggc gattgttcag ggacggcgaa tatacgctgc aactagacac ccgctccggt
    36841 acaccaacat tgatgatttc catccagaac gccgtcgaac cggtagcaag cctggtccgt
    36901 gaatgcccga aatgggatgg attaccgctc acactggatg tcagcgccac ttttccggag
    36961 ggagccgcag tccgggatta ttacagccag caaattgcga tagtgaagaa cggtcaaata
    37021 acgttacaac ccgctgccac cagcaacggt ttactcctgc tggaacgggc agaaactgac
    37081 gcccctgccc ctttcgactg gcataacgcc acggtttact ttgtgctgac agatcgtttc
    37141 gaaaacggcg atcccagtaa tgaccagagt tacggacgtc ataaagacgg tatggcagaa
    37201 attggcactt ttcacggcgg cgatttacgc ggcctgacca acaaactgga ttacctccag
    37261 cagctgggcg tcaatgcttt atggataagt gccccatttg agcaaattca cggctgggtc
    37321 ggcggcggta caaaaggtga tttcccgcat tatgcctatc atggttatta cacacaggac
    37381 tggacgaatc ttgatgccaa tatgggcagc gaagccgatc tacggacgct ggttgatagc
    37441 gcgcatcagc gcggtattcg tattctcttt gatgtcgtga tgaaccacac cggctatgcc
    37501 acgctggcgg atatgcagga gtatcagttt ggcgcgttat atctttctgg tgacgaagtg
    37561 aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg ccgccggaca aacctggcat
    37621 agctttaacg attacattaa tttcagcgac aaaacaggct gggataaatg gtggggaaaa
    37681 aactggatcc gtaccgatat cggcgattac gacaatcctg gattcgacga tctcaccatg
    37741 tcgctagcct ttttgccgga tatcaaaacc gaatcaacta ccgcttctgg tctgccggtg
    37801 ttctataaaa acaaaatgga tacccacgcc aaagccattg acggctatac gccgcgcgat
    37861 tacttaaccc actggttaag tcagtgggtc cgcgactatg ggattgatgg ttttcgggtc
    37921 gataccgcca aacatgttga gttgcccgcc tggcagcaac tgaaaaccga agccagcgcc
    37981 gcgcttcgcg aatggaaaaa agctaacccc gacaaagcat tagatgacaa acctttctgg
    38041 atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg actactatcg ccacggcttc
    38101 gatgcgatga tcaatttcga ttatcaggag caggcggcga aagcagtcga ctgtctagcg
    38161 cagatggata cgacctggca gcaaatggcg gagaaattgc agggtttcaa cgtgttgagc
    38221 tacctctcgt cgcatgatac ccgcctgttc cgtgaagggg gcgacaaagc agcagagtta
    38281 ttactattag cgccaggcgc ggtacaaatc ttttatggtg atgaatcctc gcgtccgttc
    38341 ggtcctacag gttctgatcc gctgcaaggt acacgttcgg atatgaactg gcaggatgtt
    38401 agcggtaaat ctgccgccag cgtcgcgcac tggcagaaaa tcagccagtt ccgcgcccgc
    38461 catcccgcaa ttggcgcggg caaacaaacg acacttttgc tgaagcaggg ctacggcttt
    38521 gttcgtgagc atggcgacga taaagtgctg gtcgtctggg cagggcaaca gtaacttttc
    38581 cggcttcccg ttcgtcagta cctcgggaag ccgccaacca ggataaaatg tcagccctaa
    38641 tcagcgttgc aggataaagc accgctcact cttcaacaga ccgatttgca ccccagcaaa
    38701 tgtagcgtta ttgttacctt ccttgctaca gagttcgaca gatatcccgc tatgacattc
    38761 tccctttttg gtgacaaatt tacccgccac tccggcatta cgctgttgat ggaagatctg
    38821 aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg gcggtaatcc ggcgcagatc
    38881 ccggaaatgc aggactactt ccagacgcta ctgaccgaca tgctggaaag tggcaaagcg
    38941 actgatgcac tgtgtaacta cgacggtcca caggggaaaa cggagctact cacactgctt
    39001 gccggaatgc tgcgcgagaa gttgggttgg gatatcgaac cacagaatat tgcactaaca
    39061 aacggcagcc agagcgcgtt tttctactta tttaacctgt ttgccggacg ccgtgccgat
    39121 ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat acattggcta cgctgacgcc
    39181 ggactggaag aagatctgtt tgtctctgcg cgtccgaata ttgaactgct gccggaaggc
    39241 cagtttaaat accacgtcga ttttgagcat ctgcatattg gcgaagaaac cgggatgatt
    39301 tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta ctgacgaaga gttgctgaag
    39361 cttgacgcgc tggcgaatca acacggcatt ccgctggtga ttgataacgc ttatggcgtc
    39421 ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat ggaatccgaa tatcgtgctg
    39481 tgcatgagtc tttccaagct ggggctgcct ggctcccgct gcggcattat catcgccaat
    39541 gaaaaaatca tcactgccat caccaatatg aacggcatta tcagcctggc acctggcggt
    39601 attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg atctgctgcg cctgtctgaa
    39661 acagtcatca aaccgtttta ctaccagcgt gttcaggaaa ctatcgccat cattcgccgc
    39721 tatttaccgg aagatcgctg cctaattcat aaaccagaag gagccatttt cctctggcta
    39781 tggtttaagg atttgcccat tacgaccgag caactctacc agcgcctgaa agcacgcggc
    39841 gtgctgatgg tgccggggca caacttcttc ccgggactgg ataaaccgtg gccgcatacg
    39901 catcaatgta tgcgcatgaa ctacgtacca gagccggaga aaattgaggc gggggtgaag
    39961 attctggcgg aagagataga aagagcctgg gctgaaagtc actaaaaatt gcctgatgcg
    40021 ctacgcttat caggcctaca taatttctgc aatatattta atttgcaaga atttgtaggc
    40081 cggataaggc gtttacgccg catctggcat aaacaaagcg cactattccg gcgttgagaa
    40141 acgccggaaa acgtttcaaa caggctgctg ccgtagccga tgccgctgca ctttctcgac
    40201 atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca caggccggcc ccgtctcccg
    40261 atgccagcac agatcgcact taatcgccct cgcctttttg cgcgaagaaa cgacctccat
    40321 cgcaccaaac gggcaagcca gcatacagct tttacagcca atgcaacgtg tttgttcaac
    40381 gaaaatatgc ccatgttcgc ggcttatcgc gtcaacaggg cagacattcg cgcacggtgc
    40441 atcttcacac tgatgacagg ctactgccgt ggtccagctg tggtctttaa tgacacgaat
    40501 acgggaaata aactcgtctg gtgacaacgc agcgcaatcc tgattctcct gatgcgacac
    40561 tgcgcaagcc acttcacagg tacggcaacc gatacatttc gtcgcatccg caataataaa
    40621 ccggttcatg atcactcctt tgcactaaaa cagcagagtg ccagagagcg cgtccctact
    40681 gctttgatcc aacgaaggaa atgttaaagt tttggcactt gtccaaagcg ttatgtgatc
    40741 gcgccctggt cgtccctgac agtaaatccc agttcattag aaatagcctg cgcggtttca
    40801 cgcagtggtt tcaggagatt tttctccccc acctgtttca gacgtgatgt cgaaagcgaa
    40861 atcgacacgg cgtacggcac ccgcccatga atattaaaca ccggaacagc aatacaggag
    40921 acgccgagtt cgttttcttc tctgtccatc gccgctccgc tttcacgaat gtgcgccagt
    40981 tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg ttaacggctg gatctcatgc
    41041 tgatggtttt cccagtatga cttcacgtag tccgggtgac caaacgccat gtagatcttg
    41101 cccattgcgg aacagtagag cggcatatgc tggccaatat aggcacgggt tcgcagcatc
    41161 ccggttgtgg gttccagctt ataaatcaaa atagcgtgat cgtcttcgcg gctggagaag
    41221 ttaatggttt caccagtggc gatgttcagt gcctcaagat gcggagcggc gatatgaatg
    41281 atattcagcg aagacagcgc cttctgcccg acggcaataa atttggtagt caggcggtaa
    41341 ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact gtaatccctg caataagcga
    41401 tggacggtac tcttatttaa accagccagc tccgaaagat gcgccaacgg acaaccgttt
    41461 ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa acagactctg gcttccggct
    41521 ggacgctctt tttcctgcgc catctcgttc tctttttttc ccatcacttc tttccccatt
    41581 ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc acgatctcga cagataattt
    41641 ataaccactt gatttttatg tcttttgaaa ttcatcaatc agattgcttt gttaaaaagt
    41701 gatcgatata tttgaaatca agtttcgcat attgaaattt taagccaaaa aagcgatcaa
    41761 aaaaacaagg aagcctggga tgaaagtgac atttgagcag ttaaaagcag cctttaatcg
    41821 ggtcttaatt tcacgcggcg ttgacagcga aacggctgac gcctgtgcag agatgttcgc
    41881 ccgcaccacc gaatccggcg tttattctca cggcgttaat cgtttccctc gtttcattca
    41941 acaactggaa aacggcgata tcattcctga tgcccaaccc aaacgtataa ccagcctcgg
    42001 cgcaattgaa cagtgggatg cccagcgttc gatcggcaac ctgacggcga aaaagatgat
    42061 ggatcgcgcc attgaactgg ctgccgatca cggtattggt ctggtggcac ttcgtaatgc
    42121 caaccactgg atgcgcggcg gcagctacgg ctggcaggcg gcggaaaaag gctatattgg
    42181 catttgctgg accaactcca tcgccgtaat gccgccgtgg ggcgcaaaag agtgtcgcat
    42241 tggcactaac ccgctgatcg tcgccattcc ttccacgccg atcaccatgg tcgatatgtc
    42301 gatgtcgatg ttctcttacg gcatgttaga agttaaccgc ctggcgggcc gtcagctccc
    42361 ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa gaacctggcg ttatcgagaa
    42421 gaatcgccgc attttgccga tgggctactg gaaaggttct ggcatgtcga ttgtgctgga
    42481 tatgatcgct actctccttt ccgacggcgc atcggttgcc gaagtcaccc aggacaacag
    42541 cgacgaatac ggcatttcac aaatttttat tgccattgaa gtggacaagc ttatcgacgg
    42601 tcccacccgc gatgccaagc tgcaacgtat catggattac gttactaccg ctgaacgcgc
    42661 tgacgaaaac caggccatcc gcttacccgg ccacgaattt actaccctgc
//
LOCUS       DAJSKJ010000013        41685 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000013 DAJSKJ010000000
VERSION     DAJSKJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.013"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(93..3242)
                     /gene="acrB"
                     /locus_tag="ON345_000623"
     CDS             complement(93..3242)
                     /gene="acrB"
                     /locus_tag="ON345_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HCR3070733.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTLQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3265..4458)
                     /gene="acrA"
                     /locus_tag="ON345_000624"
     CDS             complement(3265..4458)
                     /gene="acrA"
                     /locus_tag="ON345_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR3070734.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4600..5247
                     /gene="acrR"
                     /locus_tag="ON345_000625"
     CDS             4600..5247
                     /gene="acrR"
                     /locus_tag="ON345_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR3070735.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5375..8737
                     /gene="mscK"
                     /locus_tag="ON345_000626"
     CDS             5375..8737
                     /gene="mscK"
                     /locus_tag="ON345_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR3070736.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8949..9110)
                     /gene="rsmS"
                     /locus_tag="ON345_000627"
     CDS             complement(8949..9110)
                     /gene="rsmS"
                     /locus_tag="ON345_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR3070737.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9124..9651)
                     /gene="priC"
                     /locus_tag="ON345_000628"
     CDS             complement(9124..9651)
                     /gene="priC"
                     /locus_tag="ON345_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR3070738.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9721..10098
                     /locus_tag="ON345_000629"
     CDS             9721..10098
                     /locus_tag="ON345_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR3070739.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10251..10802
                     /gene="apt"
                     /locus_tag="ON345_000630"
     CDS             10251..10802
                     /gene="apt"
                     /locus_tag="ON345_000630"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR3070740.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10931..12862
                     /gene="dnaX"
                     /locus_tag="ON345_000631"
     CDS             10931..12862
                     /gene="dnaX"
                     /locus_tag="ON345_000631"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR3070741.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12195..12259
                     /gene="dnaX"
                     /locus_tag="ON345_000631"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12915..13244
                     /locus_tag="ON345_000632"
     CDS             12915..13244
                     /locus_tag="ON345_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR3070742.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13244..13849
                     /gene="recR"
                     /locus_tag="ON345_000633"
     CDS             13244..13849
                     /gene="recR"
                     /locus_tag="ON345_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR3070743.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIRLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13959..15833
                     /gene="htpG"
                     /locus_tag="ON345_000634"
     CDS             13959..15833
                     /gene="htpG"
                     /locus_tag="ON345_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR3070744.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16014..16658
                     /gene="adk"
                     /locus_tag="ON345_000635"
     CDS             16014..16658
                     /gene="adk"
                     /locus_tag="ON345_000635"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR3070745.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16894..17856
                     /gene="hemH"
                     /locus_tag="ON345_000636"
     CDS             16894..17856
                     /gene="hemH"
                     /locus_tag="ON345_000636"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR3070746.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17853..18812)
                     /gene="aes"
                     /locus_tag="ON345_000637"
     CDS             complement(17853..18812)
                     /gene="aes"
                     /locus_tag="ON345_000637"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR3070747.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRTYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18964..20268
                     /gene="gsk"
                     /locus_tag="ON345_000638"
     CDS             18964..20268
                     /gene="gsk"
                     /locus_tag="ON345_000638"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR3070748.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20401..22077)
                     /locus_tag="ON345_000639"
     CDS             complement(20401..22077)
                     /locus_tag="ON345_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR3070749.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLALVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22315..23535)
                     /gene="fsr"
                     /locus_tag="ON345_000640"
     CDS             complement(22315..23535)
                     /gene="fsr"
                     /locus_tag="ON345_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR3070750.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRRKD"
     gene            23752..25404
                     /gene="ushA"
                     /locus_tag="ON345_000641"
     CDS             23752..25404
                     /gene="ushA"
                     /locus_tag="ON345_000641"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR3070751.1"
                     /translation="MKLLQRGVALALLTTFALASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     VQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25441..25920)
                     /gene="ybaK"
                     /locus_tag="ON345_000642"
     CDS             complement(25441..25920)
                     /gene="ybaK"
                     /locus_tag="ON345_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR3070752.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26124..26918)
                     /locus_tag="ON345_000643"
     CDS             complement(26124..26918)
                     /locus_tag="ON345_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR3070753.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27056..27397
                     /locus_tag="ON345_000644"
     CDS             27056..27397
                     /locus_tag="ON345_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR3070754.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27612..30116)
                     /gene="copA"
                     /locus_tag="ON345_000645"
     CDS             complement(27612..30116)
                     /gene="copA"
                     /locus_tag="ON345_000645"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR3070755.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30378..31310
                     /gene="glsA"
                     /locus_tag="ON345_000646"
     CDS             30378..31310
                     /gene="glsA"
                     /locus_tag="ON345_000646"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR3070756.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31313..32605
                     /locus_tag="ON345_000647"
     CDS             31313..32605
                     /locus_tag="ON345_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3070757.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            32730..33137
                     /gene="cueR"
                     /locus_tag="ON345_000648"
     CDS             32730..33137
                     /gene="cueR"
                     /locus_tag="ON345_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR3070758.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33138..33596)
                     /locus_tag="ON345_000649"
     CDS             complement(33138..33596)
                     /locus_tag="ON345_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR3070759.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33593..34510)
                     /locus_tag="ON345_000650"
     CDS             complement(33593..34510)
                     /locus_tag="ON345_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR3070760.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34656..35333
                     /gene="fetA"
                     /locus_tag="ON345_000651"
     CDS             34656..35333
                     /gene="fetA"
                     /locus_tag="ON345_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR3070761.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35320..36099
                     /gene="fetB"
                     /locus_tag="ON345_000652"
     CDS             35320..36099
                     /gene="fetB"
                     /locus_tag="ON345_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR3070762.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36162..37016)
                     /gene="cnoX"
                     /locus_tag="ON345_000653"
     CDS             complement(36162..37016)
                     /gene="cnoX"
                     /locus_tag="ON345_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR3070763.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37077..37886)
                     /gene="ybbO"
                     /locus_tag="ON345_000654"
     CDS             complement(37077..37886)
                     /gene="ybbO"
                     /locus_tag="ON345_000654"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR3070764.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(37876..38499)
                     /gene="tesA"
                     /locus_tag="ON345_000655"
     CDS             complement(37876..38499)
                     /gene="tesA"
                     /locus_tag="ON345_000655"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR3070765.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            38470..39156
                     /locus_tag="ON345_000656"
     CDS             38470..39156
                     /locus_tag="ON345_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3070766.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39153..41567
                     /locus_tag="ON345_000657"
     CDS             39153..41567
                     /locus_tag="ON345_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3070767.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKALGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         9921 a        10991 c        11114 g         9659 t
ORIGIN      
        1 agatcctgag ttggtggttc aattactcct taatgttcgt aggttatgca taaaaaaggc
       61 cgcttgcgcg gccttagtga ttacacgttg tatcaatgat gatcgacagt atggctgtgc
      121 tcgatatctt cattcttgcg gctaaagcgg cggcgaacca ccacaaagaa taccggaacg
      181 aagaagattg ccagtaccgt tgcggtcacc atcccgccca ttacaccggt acctactgcg
      241 ttctgcgcgc cggaaccagc accagtactg ataaccagcg gcataacgcc gaggataaac
      301 gccagcgagg tcatcaggat cggacgtaaa cgcatccgca ctgcatcaag cgtcgcttca
      361 atcagacctt taccttcttt atccatcaag tctttggcga attcgacgat aagtatcgcg
      421 ttcttcgccg acaacccaat ggttgtgagc aggcctacct ggaagtaaac gtcattggtc
      481 aggccacgga aggtggcagc cagcaacgca ccgataaccc ccagcggaac gaccagcata
      541 acggagaacg gaatcgacca gctctcgtac agcgccgcca gacacaggaa tacgacaatc
      601 aacgaaatcg cgtacagtga aggtgcctgg ttgccggaga ggcgttcctg ataggacatc
      661 cccgtccagt catagccaac accggtaggc agtttgctcg ccagttgttc cattagctcc
      721 attgcttcac cggtactttt acccggtgcc gcctggccta agatttccat ggatggcagg
      781 ccattgtaac gttccagacg cggcgaaccg tactcccaac gagaagagga gaatgccgag
      841 aacggcacca tctgaccatc agcagcacga acataccagt cgccgatatc atccggcagc
      901 atacggtatt tcgcttctga catgacgtaa actttcttca cacgaccgcg gtcgataaag
      961 tcgttcacat agctaccgcc ccatgcagcg cccagagtgg tgttaatgtc attgatagaa
     1021 acacccagcg cctgcgcttt ttcctggtcg atatcaatct taaactgcgg ggtatcttcc
     1081 agaccgtttg gacgtacgct ggtcaacata tcagggtgct tcgctgcttc tgcaagcaac
     1141 tggttacgcg cctgagtcag tttttcgtga ccaaggccag cctggtcaat cagctcaaag
     1201 tcaaagccgg ttgcagtacc cagttccacg attgcgggca ggttaaaggc gaaaaccatc
     1261 gcatctttga tttgcgagaa agcgcgtgtt gcacgcatgg taatcgcttc aactttgttt
     1321 tcttcgcccg gacgatcggc ccagtccttc aaggaaacga acgcaatacc ggtattctga
     1381 ccacgtcccg caaagccgaa gccgttaacg gcgaacaccg actcaacgtt gttcttttct
     1441 ttggtcagat agtaatgcgt tacctcattg agcactttct gcgtacgttc ctgcgttgca
     1501 cctgctggca gctgaaccat ggtcataaat acgccctggt cctcatctgg caagaaggag
     1561 cttggcagac gcacgaacag ataggccatg ccgaccacga tgatcagata cagcaccagg
     1621 taacgccccg tactgcgcag aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc
     1681 tcgaacatgc ggttaaacca gccgaagaag ccttttttac cttccccgtg atcgcctttg
     1741 gcaatcggtt tcagcatggt ggcacaaaga gccggagtca ggatcaacgc taccagtacc
     1801 gacagcgcca ttgctgaaac aatggtaata gagaactgac gatagatagc accagtagaa
     1861 ccgccaaaga aggccatcgg tacgaatacc gccgacagta ccatcgcgat accgaccaga
     1921 gcgccctgaa tctgccccat cgacttacgg gtagcttctt ttggcggcaa accttcttcc
     1981 gccataacac gctccacgtt ttctaccaca acgatagcgt catccaccaa caatccgatg
     2041 gcgagcacca tcccgaacat tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg
     2101 gcaaaggtcc cgagcaatac caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag
     2161 ttctgcagga acagatacat aaccaggaac acgaggatga tcgcttcgac cagcgtttta
     2221 accacttcgt gaatagagat tttcacgaac ggcgtggtgt cgtacgggta aacaattttc
     2281 agacccgacg ggaagaacgg ttccatcttc gccagttcag cacggattgc cgcagcggta
     2341 tccagcgcgt ttgcaccggt cgccagcttg atccccagac cggaagccgg ttggccgtta
     2401 aactctgcga tgatgtcgta gttctcacca cccagctcaa tcttcgccac atcacgcagc
     2461 agtacgcggg aaccatcctg attcactttc agcaggattt tgccgaactc ttcagtagag
     2521 gtcagacgcg tctgagcaat aatagaggcg ttaagctgtt ggcctttcac cggcggcgta
     2581 ccaccgagct gacccgccgc aacctgggcg ttctgcgctt tgatggcggt aatgacatca
     2641 accggcgtta gctggaattt gttcagctca ttcgggttca tccagatacg catcgcgtac
     2701 tgtgaaccga acaactgaac atcacccacg cccgacgtac ggctgatggc atctttcata
     2761 ttcgccgcta cgtagtcaga gatatcctcc tgcgtcatgg tgccatcggt gttgataacg
     2821 ccgacaacca tcaggaagct gctggatgat ttctcaacgc tcaccccttg ctgctgaact
     2881 tcttgcggca gcaacggcat cgccagctgc agtttgttct gcacctgaac ctgcgcgata
     2941 tccgcatcag taccagactc aaaggtcagg gtgatctgca gggtacccgt ggagtcactg
     3001 ttagaggaca tgtacatcag gttatcgata ccgttcatat tctgttcgat aacctgtgtc
     3061 accgtgtcct gcactgtttt cgcatcagcg ccggggtagg aggcggagat cgttactgcc
     3121 ggcggtgcaa tcgtaggata ttgcgccacc ggcagtttga ggatcgccag cccccctgcc
     3181 aacatgatga taatggcgat cacccacgca aaaatcgggc gatcgataaa gaaattaggc
     3241 atgtcttaac ggctcctgtt taagttaaga cttggactgt tcaggctgag caccgcttgc
     3301 ggcttgctgg ttattatcag cggtaacttc ttgtgctttt acctggacac caggacgcac
     3361 tttctgcagc ccacttatta ctacgcgatc gcctgctttc agaccttctg tcaccagcca
     3421 cttatcgcca atagcctggc ttgcaacgat cggacgggtt tccactttgt catccgcgcc
     3481 aactaccagt acggtggcat caccacgcgg cgtacgggtt acgccctgtt gcgggactaa
     3541 aatagcgttt ggattaagcc cttcttccag acgtgcgcgc acgaacatac ccggcagcag
     3601 agtgtgatcc gggttcggga agatagcgcg tagggtgata gacccagtgg tctgatcaac
     3661 ggtaacgtca gagaattcca gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat
     3721 cagcgacact ttggctttgc cgttctcttg tttcagcgtg ccattcgcca gttcctgttt
     3781 caggcgcagg aagtcgttgc tggactgggt cacatcaacg tagatcggat caagttgctg
     3841 tacggttgcc agcgcagtcg cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga
     3901 cttaccaatg cgaccgctaa tcggagaggt gactttggtg taagccagat tgatccgcgc
     3961 agtttcaacg gcagctttcg ccgcagttac cgcagcattc gcctgttgcg catcagccag
     4021 agcctgatcg tactcttgct tactgatgta ctgagtaccg agcagtttct gataacgatt
     4081 caccgtcaat tgcgcgatat tggctgcagc ctgggctttc gccagatcac ctttcgcact
     4141 gtcgtatgtc gcctgatagg tcgcaggatc aatctgatag agagagacac cagcttcgat
     4201 gtcgctacct tctttgaaat tacgcttcag gataatcccg ctaacttgag gacgaacttc
     4261 tgcgatccgg taggcactgg tgcgacccgg aagctcggtt gtgatctgca gaggttcagt
     4321 tttgactgtt actacgccaa cggcgggcat ctgctggcca ccttgttggg cctgtttgtc
     4381 gtcacatcct gttagggcta agctgcctga gagcatcaga acgaccgcca gaggcgtaaa
     4441 ccctctgttt ttgttcatat gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca
     4501 aaagttaata aacccattgc tgcgtttata ttatcgtcgt gctatggtac atacattcac
     4561 aaatgtatgt aaatctaacg cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa
     4621 gaagcgcaag aaacgcgcca acacatcctc gatgtggctc tacgtctttt ctcacagcag
     4681 ggggtatcat ccacctcgct gggcgagatt gcaaaagcag ctggcgttac gcgcggtgca
     4741 atctactggc attttaaaga caagtcggat ttgttcagtg agatctggga actgtcagaa
     4801 tccaatattg gtgaactaga gcttgagtat caggcaaaat tccctggcga tccactctca
     4861 gtattaagag agatattaat tcatgttctt gaatccacgg tgacagaaga acgacgtcga
     4921 ttattgatgg agattatatt ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa
     4981 caggcacaac gtaatctctg tctggaaagt tatgaccgta tagaacaaac gttaaaacat
     5041 tgtattgaag cgaaaatgtt gcctgcggat ttaatgacgc gtcgggcagc aattattatg
     5101 cgcggctata tttccggcct gatggaaaac tggctctttg ccccgcaatc ttttgatctt
     5161 aaaaaagaag cccgcgatta cgtcgccatc ttactggaga tgtatctcct gtgccccacg
     5221 cttcgtaatc ctgccactaa cgaataaccc tgaatctgac tccaggattt ttcctggaca
     5281 ttttcgtcgt tgctattctg gttcactgcg tcgtgatatt cttgcggttt gactttttca
     5341 ggtcgttctt caggttcaga aaccttcatt catcatgact atgttccagt attacaaacg
     5401 atcacggcat tttgtttttt cagcatttat tgcttttgtt tttgtcttgt tatgccagaa
     5461 cacggcgttt gcgcgggcgt catcgaatgg tgatctgccg acaaaagcgg acctgcaggc
     5521 gcaacttgac tcactaaata aacaaaaaga tctttctgct caggacaaac tggtgcagca
     5581 ggatctgaca gatacattag ccaccctcga taaaatcgat cgcataaaag aagagacagt
     5641 tcagctacgg caaaaagtcg ctgaagcgcc ggaaaaaatg cgccaggcga ccgcggcgtt
     5701 aacagcactt agcgatgtcg ataacgacga agaaacgcgc aaaattctga gtacgctttc
     5761 gttgcgccag ctggaaactc gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa
     5821 cgatctggcg tcttataaca gccagctggt ttcgttacag acgcagcccg aacgcgtgca
     5881 aaatgcgatg tataacgctt cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac
     5941 tgatgtcggc gagactgcct tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc
     6001 gttgctgaat gcggagattg accagcagcg taaaagcctg gaagggaaca ccgtcttgca
     6061 ggataccttg caaaagcaac gtgattacgt gacggcgaac agcgcccgtc tggagcacca
     6121 gttgcagctg ttgcaagaag cggtaaacag caagcgcctg actttaactg aaaaaacggc
     6181 gcaggaagcc gtctccccgg atgaagccgc gcgtattcag gctaatccgc tggtgaaaca
     6241 ggaactggaa attaaccagc agttaagtca gcgtctgatt accgcgactg aaaacggtaa
     6301 tcagttgatg cagcaaaaca ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga
     6361 acgcaatatt aaagagcaga ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct
     6421 ttaccagcaa caacaaacgc tgccctcggc ggatgaactg gaaaacatga ccaaccgcat
     6481 cgcggatttg cgtctcgaac agtttgaagt taaccagcag cgtgatgcac ttttccagag
     6541 cgatgcgttc gtcaacaaac tggaagaagg tcacaccaac gaagtcaaca gcgaagttca
     6601 cgatgcgtta ttgcaagtgg ttgatatgcg tcgcgaattg ctggatcaac tcaacaaaca
     6661 gttgggtaac cagctgatga tggccattaa cctgcaaatc aaccagcagc agttaatgag
     6721 cgtatcgaaa aacctgaaat ccatcctgac tcagcaaatc ttttgggtga acagtaaccg
     6781 tccaatggac tgggactgga tcaaagcgtt cccgcaaagc ctgaaagatg aatttaagtc
     6841 gatgaaaatc acggtgaact gggaaaaagc ctggcctgcc gtttttatcg ctttcctcgc
     6901 cggtttgccg ctgctgttga ttgccgggct gatccactgg cgtctgggct ggctgaaagc
     6961 gtatcaacag aaactggctt ccgctgtggg ttccctgcgt aacgatagcc agctcaacac
     7021 accaaaagcg atccttatcg acctgatccg cgcgctgccg gtgtgcctga ttattctcgc
     7081 ggttggcctg attctgttga ccatgcagct caacatcagc gaactgctat ggtcgttcag
     7141 taaaaaactg gcgattttct ggctggtgtt tggcctgtgc tggaaggtac tggagaaaaa
     7201 tggcgttgcc gtgcgtcact tcggcatgcc ggagcagcag accagccact ggcgtcggca
     7261 aattgtccgc atcagtctcg cgttgctgcc tatccatttc tggtctgtgg tggcagaact
     7321 ttccccgctg catctgatgg atgatgtgct ggggcaggcg atgattttct tcaacctgct
     7381 gctgattgcc ttcctggtat ggccaatgtg ccgcgaaagc tggcgtgata aagagtcgca
     7441 caccatgcga ctggtcacca ttaccgtgct gtcgattatc ccgattgcat tgatggtgct
     7501 gactgccacg ggttatttct acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt
     7561 ttatctggtg atcatctgga acttgctcta ccagactgta ttgcgcggct taagcgtagc
     7621 ggcgcggcgt atcgcctggc gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg
     7681 cgcagaaggt gccgaaccgc cggaagaacc caccattgca ctggaacagg ttaaccagca
     7741 gacgctgcgt attaccatgt tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat
     7801 ttggtccgat ttgatcaccg tgttcagcta tctcgacagc atcacgctct ggcattacaa
     7861 cggcactgaa gctggcgctg cggtggtgaa aaacgtcacc atgggcagtc tgttgtttgc
     7921 gattatcgcc tcaatggtgg cctgggcgtt gattcgcaac ctgcctggtt tactggaagt
     7981 gctggtgctc tcgcgactga atatgcgtca gggcgcgtcg tatgccatta ctaccatcct
     8041 taactacatc attattgccg ttggtgcgat gacggtgttc ggatcgctgg gcgtctcttg
     8101 ggataaactc cagtggctgg ccgcagcatt atccgtaggt cttggttttg gtttacaaga
     8161 aattttcggt aacttcgtct ccggtttgat cattctattc gaacgtccgg tgcgtattgg
     8221 cgatacggta accattggta gcttctcggg gacggtaagt aagatccgta ttcgtgcgac
     8281 aacgattacc gatttcgatc gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga
     8341 gcgtctgatc aactggtcgt tgactgacac tactacgcgt ctggtgatcc gtctcggcgt
     8401 ggcctatggc tccgatctgg aaaaagtgcg taaagtgtta ctgaaggcgg cgactgagca
     8461 cccaagggtg atgcacgaac caatgccgga agtcttcttt acggcgtttg gtgccagcac
     8521 gttggatcat gagctgcgtc tgtatgtgcg tgaactgcgt gaccgtagtc gtactgtcga
     8581 tgagctgaac cgtactatcg atcagctgtg ccgtgaaaac gacatcaaca ttgcctttaa
     8641 ccagcttgaa gtgcatctgc acaacgagaa gggcgatgag gtgacggaag tcaaacgcga
     8701 ctacaaaggc gatgacccga cgccagcggt agggtaataa ccaaaggggg cgacatttag
     8761 ttgccccgag attactgaca aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt
     8821 atccggccta cgaaaccgca agaattcaat gtattgcagg agcggtgtag gcctgataag
     8881 cgtagcgcat caggcagttt tgcgtttgcc cacaatctta ggggacattt agcggcccca
     8941 tttatttctc acttttccgc ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt
     9001 acaatatcca gcgcgcgcag cacggtgcgg gctgggatct gattttcttc cagcagcaca
     9061 atcaaatcga cagccagttt gacatcgtca ggggcatttt ccagtgacat actctctcca
     9121 ttgctagcgg gttaagcgcg ctagcctgtt ttcgattttt tccagcgcat ggcggcagcg
     9181 tgccaggcgc gcttcatagg cttccacttc acgatgcagc gtttgctgtt ccacgagatc
     9241 ggtcacccgc gccaggcggg ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa
     9301 atcctgatgc tgaatacgtt tacgctgcca gcgggcaatt ttcggtggcg cactgtccca
     9361 ctcgcgcaat gaccaggcgg aggcttcacg cgcgatggct tccagttgtg ccgccagatg
     9421 ttccgccagc caggccactt gcggcagctg ttgctgctca actgcatgac gaagcgcagc
     9481 cagattatcg cccgcctcat cgagacaagc ctgcagtgtt gtcgcacggg tttgaaaaag
     9541 gtgtctgtca aaacgggcac ttagcgtggc aaattgcgct accggggcac aacgctgacg
     9601 cagcgtagcg agctgtcctt ccagtttttc cagcagcagg gcggttttca cgataagacc
     9661 tcaatgaaaa tgataattgt tatgctaaag tagccactct gtaagctgac aacactcaat
     9721 atgcaacgaa tcattttaat catcattggc tggctggcgg tagtgctggg tacgctgggc
     9781 gtggtattac cggtattacc gacgacgccg tttatcctgc tggcggcctg gtgctttgcc
     9841 cgttcttccc cgcgctttca cgcctggttg ctgtaccgct catggtttgg cagctatcta
     9901 cgtttctggc agaaacatca tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt
     9961 attttgctca cgtttgccat ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg
    10021 ttgctggtaa ttctcgcctg tttgcttttc tatatgtggc gaattccggt gattgatgaa
    10081 aagcaagaaa agcactgaag cacaacaatc gcagttgcaa ttattgcgta cagccagtac
    10141 attctggcgt tttcgagcac aggcgcaggc ggtcgaaggt taaacaactg ttacttttga
    10201 tacgtttaaa acgcgccgtg agtaccaccg taacaagcag gcatacactt atgaccgcga
    10261 ctgcacagca gcttgagtat ctcaaaaata gcatcaaaag cattcaggac tacccaaaac
    10321 ccggcattct tttccgcgat gtcaccagct tactggaaga cccgaaagct tacgctctca
    10381 gcatcgactt gctggttgag cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg
    10441 aagcgcgtgg cttcttgttt ggcgctccgg tagctctggg tctgggcgtt ggctttgtac
    10501 cggtccgtaa accgggcaaa ctgccgcgtg aaaccatcag tgaaacttac gacctggaat
    10561 acggcaccga tcagctggag atccacgttg atgccatcaa accgggcgac aaagttctgg
    10621 tggtggacga tctgctggca accggcggca ctatcgaagc gaccgttaaa ctgatccgtc
    10681 gtctgggtgg tgaagtggct gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg
    10741 aacagcgtct cgaaaaacag ggcattacca gctacagcct tgtcccgttc ccgggccatt
    10801 aattatcgcc agtcttgtgc tgcccacgct acggacagca caagatgtgc attcagcctc
    10861 gccgttctga cggggctgtg ttagcattac cccttcgtga atccaccttc cagcgtttca
    10921 gagcctgcca atgagttatc aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga
    10981 cgtcgtcggc caggaacatg tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat
    11041 tcatcatgct tatctttttt ccggcacccg tggcgtcgga aaaacctcta tcgcccgact
    11101 gctggcgaag gggctaaact gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga
    11161 taactgtcgt gaaatcgagc aggggcgctt tgtcgatctg attgaaatcg acgccgcctc
    11221 gcgcaccaaa gttgaagata cccgcgacct gctggataac gtccagtacg ctccggcgcg
    11281 tggtcgtttc aaagtttatc tgatcgacga agtgcatatg ctgtcgcgcc acagctttaa
    11341 cgcattgtta aaaacccttg aagagccgcc ggagcacgtt aagtttctgc tggcgacgac
    11401 cgatccacag aaattgccgg tgacgatttt gtcacgctgt ctgcaatttc atctcaaggc
    11461 gctggatgtc gagcaaattc gccatcagct tgagcacatc ctcaacgaag aacatatcgc
    11521 tcacgagccg cgggcgttgc aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc
    11581 cttaagtctg accgaccagg cgattgccag cggtgacggc caggtttcaa cccaggcggt
    11641 cagtgcgatg ctgggtacgc ttgacgacga tcaggcgctg tcgctggttg aagcgatggt
    11701 cgaggccaac ggcgagcgcg taatggcgct gattaatgaa gccgctgccc gtggtatcga
    11761 gtgggaagcg ttgctggtgg aaatgctcgg cctgttgcat cgtattgcga tggtacaact
    11821 ttcgcctgct gcacttggca acgacatggc cgccattgag ctgcggatgc gtgaactggc
    11881 gcgcaccata ccgccgacgg atattcagct ttactatcag acgctgttga ttggtcgcaa
    11941 agaattaccg tatgcgccgg accgccgcat gggcgttgag atgacgctgc tgcgcgcgct
    12001 ggcgttccat ccacgtatgc ctctgcctga gccagaagtg ccacgccagt cctttgcgcc
    12061 cgtcgcgcca acggcagtaa tgacgccaac ccaggtgccg ccgcaaccgc aatcagcgcc
    12121 gcagcaggca ccgactgtac cgctcccgga aaccaccagc caggtgctgg cggcgcgcca
    12181 gcagttgcag cgcgtgcagg gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac
    12241 ccgcgcgcgg ccggtgaata acgctgcgct ggaaagactg gcttcggtca ccgatcgcgt
    12301 tcaggcgcgt ccggtgccat cggcgctgga aaaagcgcca gccaaaaaag aagcgtatcg
    12361 ctggaaggct accactccag tgatgcagca aaaagaagtg gtcgccacgc cgaaggcgct
    12421 gaaaaaagcg ctggaacatg aaaaaacgcc ggaactggcg gcgaagctgg cggcagaagc
    12481 cattgagcgc gacgcgtggg cggcacaggt gagtcaactt tcgctaccaa aactggtcga
    12541 acaggtggcc ttaaatgcct ggaaagagga gagcgacaac gcagtatgtc tgcatttgcg
    12601 ctcctctcag cggcatttga acaaccgcgg tgcacagcaa aaactggctg aagcgttgag
    12661 catgttaaaa ggttcaacgg ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg
    12721 tacgccgctg gagtggcgtc aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc
    12781 cattattgcg gataataata ttcagaccct gcgtcggttc ttcgatgcgg agctggatga
    12841 agaaagtatc cgccccattt gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat
    12901 tgagagagaa acctatgttt ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc
    12961 agatgcaaga aaaaatgcag aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg
    13021 aatctggcgc aggtctggta aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag
    13081 agatcgaccc gagcctgctg gaagacgaca aagagatgct ggaagacctg gtggctgcag
    13141 cattcaacga cgcagcacgt cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat
    13201 cctccggaat gcagctgccg cctggcttta agatgccgtt ctgatgcaaa ccagcccgct
    13261 gttaacacag cttatggaag cactgcgctg tctgccgggc gttggcccga agtcggcgca
    13321 gcgtatggcg ttcacgctgc ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc
    13381 gctcacccgg gcgatgtcgg aaatcggcca ctgcgccgat tgccgcactt tcactgaaca
    13441 ggaagtctgt aacatctgtt cgaatccgcg tcgtcaggaa aacggtcaaa tctgtgtggt
    13501 ggagagtccg gcggacatct acgccattga gcagacgggg cagttttcag gccgttattt
    13561 tgtgttgatg gggcatctgt caccgctgga cggcatcggt ccggatgata tcaggcttga
    13621 tcgtctggaa cagcgtctgg cagaggaaaa aatcactgaa gtgatcctcg ccaccaatcc
    13681 cacggttgaa ggtgaagcta ccgctaacta cattgccgag ctttgcgcgc aatatgacgt
    13741 ggaagccagc cgaatcgctc atggcgtacc ggttggcggc gaactggaaa tggtcgatgg
    13801 caccacgctg tcacactccc ttgccgggcg tcataagatt cgtttttaag caaacgagag
    13861 caggatcacc tgctctcgct tgaaattatt ctcccttgtc cccatctctc cctcatcctg
    13921 tttttaacct taaaatggca ttattgaggt agacctacat gaaaggacaa gaaactcgtg
    13981 gttttcagtc agaagtgaaa cagcttctgc acctgatgat ccattctctc tattccaata
    14041 aagaaatctt cctgcgtgag cttatctcta acgcctccga tgcggcggac aagctgcgtt
    14101 tccgtgcgct atctaacccg gacctgtacg aaggtgatgg cgaactgcgc gttcgtgtct
    14161 ctttcgataa agacaagcgc acgctgacca tctccgataa cggcgtgggg atgacccgcg
    14221 acgaagtgat tgaccatctg gggactatcg ctaaatccgg taccaaatca ttcctcgaat
    14281 ccctgggttc tgaccaggcg aaagacagcc agctgatcgg tcagtttggt gttggtttct
    14341 actctgcgtt tatcgtggcc gacaaagtga ccgtgcgtac tcgcgcggca ggcgaaaaac
    14401 cagaaaacgg cgtcttctgg gaatcggctg gcgaaggtga atataccgtt gccgacatca
    14461 ccaaagaaga tcgtggtact gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc
    14521 tcgatgactg gcgcgtgcgt tccatcatca gcaaatactc cgaccatatc gcgctgccgg
    14581 tagagatcga aaaacgcgaa gagaaagacg gcgaaaccgt tatctcctgg gagaaaatca
    14641 acaaagcgca ggcgctgtgg actcgtaaca agtcggaaat caccgatgaa gagtacaaag
    14701 agttctacaa acacatcgcc cacgacttta atgatccgct gacctggagc cacaaccgtg
    14761 ttgaaggtaa gcaggagtat accagcctgc tgtacatccc gtcccaggct ccgtgggata
    14821 tgtggaaccg cgatcataaa cacggcctga aactgtatgt tcagcgtgtg ttcatcatgg
    14881 acgacgcaga acagttcatg ccgaactatc tgcgcttcgt gcgtggtctg attgactcca
    14941 gcgatctgcc gctgaacgtt tcccgtgaaa tcctccagga cagcacggta acgcgtaacc
    15001 tgcgcaatgc gctgaccaag cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg
    15061 cggaaaaata ccagaccttc tggcaacagt ttggcctggt actgaaagaa ggtccggcgg
    15121 aagatttcgc taaccaggaa gcgatcgcca aactgctgcg ttttgcttct acccataccg
    15181 attcttctgc gcagaccgta tctctggaag actacgtttc ccgcatgaaa gaagggcagg
    15241 agaaaatcta ctacatcacc gcagacagct atgcggcagc gaagagcagc ccgcacctgg
    15301 aactgctgcg taagaaaggc atcgaagttc tgctgctttc cgaccgcatc gatgagtgga
    15361 tgatgaacta tctgactgag ttcgacggta aaccgttcca gtcggtgtct aaagttgacg
    15421 agtcgcttga aaaactggct gacgaagttg atgagagcgc gaaagaagcg gagaaagcac
    15481 tgactccgtt catcgaccgt gtgaaagccc tgctcggcga gcgcgtgaaa gatgtccgtc
    15541 tcactcaccg tctgaccgat acgccagcga tcgtttcgac cgacgcggac gaaatgagca
    15601 ctcagatggc gaaactgttc gctgcggcgg gccagaaagt gccagaagtg aaatacatct
    15661 tcgaactgaa cccggatcac gtactggtga aacgtgcggc agatactgaa gatgaagcga
    15721 agttcagcga gtgggtagaa ctgctgctgg atcaggcgct gctggcagaa cgcggcacgc
    15781 tggaagatcc gaacctgttt attcgtcgta tgaaccagct gctggtttcc tgatgtcatg
    15841 ccggatgacc tttgtgtcat ccggcatttt tcttttcatc atctgcactt tccgcaaatt
    15901 atctcgccat taaccgtttc agcctcaggt gcctttcttg aggcaatcgc ctgttggtgg
    15961 tatcgtttat cgctttttca aaaaaatcga cacattttaa ggggattttc gcaatgcgta
    16021 tcattctgct tggcgctccg ggcgcgggga aagggactca ggctcagttc atcatggaga
    16081 aatatggtat tccgcaaatc tccactggcg atatgctgcg tgctgcggtc aaatctggct
    16141 ccgagctggg taaacaagca aaagacatta tggatgctgg caaactggtc accgacgaac
    16201 tggtgatcgc gctggttaaa gagcgcattg ctcaggaaga ctgccgtaat ggtttcctgt
    16261 tggacggctt cccgcgtacc attccgcagg cagacgcgat gaaagaagcg ggcatcaatg
    16321 ttgattacgt tctggaattc gacgtaccgg acgaactgat cgttgaccgt atcgtcggtc
    16381 gccgcgttca cgcgccgtct ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag
    16441 aaggcaaaga cgacgttacc ggtgaagaac tgactacccg taaagacgat caggaagaaa
    16501 ccgtacgtaa acgtctggtt gaataccatc agatgactgc accgctgatc ggctactact
    16561 ccaaagaagc ggaagcgggt aataccaaat acgcgaaagt tgacggcacc aagccggttg
    16621 ctgaagttcg cgctgatctg gaaaaaatcc tcggctaatt cgaaagcgcg cacggacagt
    16681 cccctcgccc cctcggggag agggttaggg tgaggggaac aggcccgcac aagcaaactt
    16741 atcagcaatc tcaggccgga tattcattcg gccttttaca aaaacctcaa tctgcgctat
    16801 tcctttttct gatttgacct ctcacagcaa ttagttcttc ttcctcactt ttccgctaca
    16861 attatcaaca agttgaatcg ataagaggcg gtaatgcgtc agactaaaac cggtatcctg
    16921 ctggcaaacc tgggtacgcc cgatgccccc acacctgaag cggtaaaacg ctatctgaaa
    16981 caatttttaa gcgacagacg cgtggttgat acctcacggt tgttatggtg gccgttgctg
    17041 cgcggcgtga ttttgccgct gcgctcgccg cgtgtggcga agctgtacgc ctcggtctgg
    17101 atggaagacg gctcgccgct gatggtttac agccgccagc aacagcaggc gttggcacaa
    17161 cgtttaccgg atacgcccgt agcgctggga atgagctacg gctcgccatc actggaaagc
    17221 gccgtcgatg aactcctggc agagcatgta gatcatattg tggtgctgcc gctttatccg
    17281 caattctcct gttctacggt cggtgcggta tgggatgaac tggcacgcat tctggcgcgc
    17341 aaacgtagca ttccggggat atcgtttatt cgtgattacg ccgataacca cgattacatt
    17401 aatgcactgg cgaacagcgt acgcgcttct tttgccaaac atggcgaacc ggatctgctg
    17461 ctgctctctt atcatggcat tccccagcgt tatgcagatg aaggcgatga ttacccgcaa
    17521 cgttgccgca caacgactcg cgaactggct tccgcactgg ggatggcacc ggaaaaagtg
    17581 atgatgacct ttcagtcgcg ctttggccgg gaaccctggc tgatgcctta taccgacgaa
    17641 acgctgaaaa tgctcggaga aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt
    17701 gctgcggatt gtctggagac gctggaagag attgccgagc aaaaccgtga ggtcttcctc
    17761 ggtgccggcg ggaaaaagta tgaatatatt ccagcgctta atgccacgcc ggaacatatc
    17821 gaaatgatgg ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg
    17881 ccgtcgcgaa gagcctcgtc ggcggttttc atcattcgtg aataatgcaa aaaggcatgc
    17941 agcgtacctg ggtagagttt gaactcacag ggctgctgat gcgccgctaa cgtctggtaa
    18001 agcagacggc tgtcatccag cagcggatcg aactccgccc cggcaataaa acagggcgga
    18061 acttcgcgag tgagatcatt attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg
    18121 ttgcttaaat acgcctcttc atacatctgc aaatcctgtt gcgttaagcc atcccagaca
    18181 ccgcccaaca gacgacgagt cacggaatcc cgtaatccgt aaagcccata ccacagcaaa
    18241 acacccgcaa ttttaccgca atcgatctgt ttatcacgca accacaacgc actggcgagc
    18301 gccagcatgg cacctgcgga atcaccggca aagccaatgc gggacatatt gatttgataa
    18361 tcctccgcct gctggtggaa ataacaacaa gcagccacaa tttcctctat cgcttgcgga
    18421 aaacgcgctt caggtgaaag ggggtaatca ataccaatca ccgtacattg gctgtagctt
    18481 gccagcaggc gcatgatgcg atcgtgggta tcgagattgc cgagaataaa accgcctcca
    18541 tgcaaataaa atagcgtcgc cgggctatct ggctgcggac aaaagagacg tgtttccacc
    18601 tgcccatatt ttgttggaac catgtaagtt ctggttgcca tttctggagc gcccgcattc
    18661 cagaatcggc gctcaagcgt gtaatactgt cgttgctcag caatcgttcc cgttgcgggc
    18721 caggacggta aatccggctg aagagtattc acaacggtct tcatttcagc agaaataagg
    18781 tccagaacag gtagtttgtt ttccggcttc atacaaaaac tcctttcaaa ttacgtaatt
    18841 gtaaggaacc actgccatga aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc
    18901 tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca
    18961 accatgaaat ttcccggtaa acgtaaatcc aaacattact tccctgtaaa tgcacgcgat
    19021 ccgctgcttc agcaattcca gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt
    19081 gaccagacgc tggtcgatat tgaagcgaaa gtggatgatg aattcattga gcgttatgga
    19141 ttaagcgccg ggcattcact ggtgattgag gatgacgtag ccgaagcgct ttatcaggag
    19201 ctaaaacaga aaaacctgat tacccatcag tttgcgggtg gcaccattgg taacaccatg
    19261 cacaactact cggtgctcgc ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat
    19321 attgaaattg gcagttatgc ctatcgttac ctgtgtaaca cttccagccg taccgatctt
    19381 aactatctac aaggcgtgga tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc
    19441 ggggaacgta cctttgctat cagtccaggc cacatgaacc agctgcgggc tgaaagcatt
    19501 ccggaagatg tgattgccgg agcctcggca ctggttctca cctcttatct ggtgcgttgc
    19561 aaaccgggtg aacccatgcc ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat
    19621 aacgtaccgg tggtgctgac gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg
    19681 tggcagcaat tcctcaaaga ccacgtttct atccttgcga tgaacgaaga tgaagccgaa
    19741 gcgttgaccg gagaaagcga tccgttgttg gcatctgaca aggcgctgga ctgggtagat
    19801 ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg cgggctttac cgaagacgaa
    19861 gcgaaacgta aaacccagca tccgctgctg ccgggcgcta tagcggaatt caaccagtat
    19921 gagtttagcc gcgccatgcg ccacaaggat tgccagaatc cgctgcgtgt atattcgcac
    19981 attgcgccgt acatgggcgg gccggaaaaa atcatgaaca ctaatggagc gggggatggc
    20041 gcattggcag cgttgctgca tgacattacc gccaacagct accatcgtag caacgtacca
    20101 aactccagca aacataaatt cacctggtta acttattcat cgttagcgca ggtgtgtaaa
    20161 tatgctaacc gtgtgagcta tcaggtactg aaccagcatt cacctcgttt aacgcgcggc
    20221 ttgccggagc gtgaagacag cctggaagag tcttactggg atcgttaagt tatcgtcggt
    20281 tcgtaggcca gataaggcgt tcacgccgca tctggcattt ggctctcgat gcccgatgcg
    20341 acgctggcgc gtcttatcat gcctacatat ttttcatatt ttacatccgg caaccaccgt
    20401 ttaccccgtc accacttcac ccgccggtgg cgtttccagc agttccagca tagtacgggc
    20461 aatttcacgt tcacccatca ctacctgatt cgcgccacgt tcggtgatat acgtcacttc
    20521 atcgtcataa tgggcgcggg cgataatctc aatatccggg tttttcgcgc gggcagacgc
    20581 cacaatctca cccgcttcat aaccgttggg gatcgtcagg atcagccatt ttgcacattc
    20641 cagatgcgcc agttgcataa tttcttcgtt cgctgcattg cccaatacag cgcggacccc
    20701 acgctctcgc agctcatcaa cacgggttcg tgacgtctca atcaccacca gcggaatatc
    20761 agaggcgagc aatttctccc ccagcaggct gcctacacga ccgtaaccca ccaatagcgc
    20821 atggttgcaa atatccactg ggatctgttt ctcttcttcg attgcctctt ccagcgtctg
    20881 ctcttccagc gtttcggtct tcgccagata tttctccagt agtgcgaaca gtaccgggtt
    20941 gagcataatc gacaggatcg cccctgccag taccaggttt tgtccggcct gcggcagtaa
    21001 attcaatgcc attcccagtc ccgccaggat aaacgcgaac tcaccaatct gcgccaggct
    21061 ggcggcgatg gttaatgccg tacgttggga gtgaccaaac agtcgcacca ggaaaaatgc
    21121 ggctaacgac ttaccaaaca gaataatcgc cagcgtcgcc agcactgcca gcggttgctg
    21181 aatcagaatt aacggatcaa acaacatccc gacggagaca aaaaacagca ccgcaaacgc
    21241 gtcgcgcaat ggcagcgtat cgtgggcggc acggtgactc agttcagact cgttcagtac
    21301 catcccggca aagaacgcac cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa
    21361 ggcaatccct aacgccagcg ccagcaccga cagggtaaac agctcgcgag aaccggttgc
    21421 cgcgctgcgt gccataatcc acggcaccag acggcgacct accagcatca taatggcgat
    21481 aaatgcgatc actttgccga tggtgatccc catatcgact gcaagagtgg caaagcccac
    21541 atcgccctgt tccatcattc ctgccactgc gggcagcaac accagcgcca gaaccattac
    21601 caggtcttcc acaatcaacc aaccgatggc gatttgccca cgctgactgt caattaattg
    21661 ccgttcttca agtgcgcgca gtaacaccac ggtactggcg gtggaaagac ataaaccgaa
    21721 cacgataccg gtcattaacg accagcccag cacggcagag agcgccatac ccagcagcgt
    21781 cgccacggct atctgggcga tcgcaccggg aatggcgatg gcctttaccg ccatcaaatc
    21841 cttcagcgaa aagtgcaaac cgacgccaaa catcaacaga atgacgccca gttcagccag
    21901 ttccggcgca agcttggtat cggcaacaaa gcccggagtg aatggtcctg ccagcacacc
    21961 cgctaacaga tatcccacca gaggagaaat acgtagttta ttggccagca tgccgaggat
    22021 aaaggcgagc acaaggccgc caacaatggt ggtgataagc ggggtggcgt gatgcattcc
    22081 gtctcctttt cctggtggtt attgtccatt tttggccggg aaaaccaaaa ttacaggtaa
    22141 tagtttatga caatttcatt gatgatgttc atgaataatt attgaatttt gcagaaaaat
    22201 ggaattagct gcaaaaaaag cacggatcgg aaaacagaag gcggtttaac agaggaaagg
    22261 cttatggcga ctgtaaaacg atgcagccaa agtttgcctt tggctgcaat gaaatcagtc
    22321 tttacgccgg ttatcaggca ggaatatggt caacatcccc aatagtggca ggaaagcaca
    22381 gattttatag actaactcga tgctggtgtg atcggcgata agccccagaa ctgccgctcc
    22441 cagacctccc atgccaaaag caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc
    22501 cggaagtagc tcctgagcgt agaccagaat ggcagagaat gccgaagcga ggataaatcc
    22561 aataatcacc gttaaaaccc ccgtccagtg caggctggcg tagggtaaaa tcagcgtaaa
    22621 cggcgcaacg ccgaggatag agccccaaat cacatatttc cgtccaattt tatcccctac
    22681 aggcccgccg atcaccgtac ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg
    22741 ggcattctgg atagataatc cgaatttttg catcagataa aaggtgtaat agctgctgat
    22801 gctcgccata tagaaatatt tcgagaaaat gaggattaac agaatgctga ctgccagtac
    22861 cactttgttg cgcggcagtg gattgattat cgtcgctttg ggttttcctt tattcattcg
    22921 gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca gcagtgccgc
    22981 aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa
    23041 ggggcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg ccaggccatg
    23101 ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga ccgatgaacc
    23161 ggtaccgacc agcgccgccg ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag
    23221 caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca tcggatattt
    23281 atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga aggtgagggt
    23341 tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagcg gataaatcgc
    23401 cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag cacctaaaat
    23461 accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag gctgggtttg
    23521 ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga atgatcttat
    23581 tgtgattatt acccgactaa catacctgta tgcgtcgtct gaaggaagtc tcaacgccga
    23641 atacagaatt tctaatctgg atgcagattt atcttcaccg gacgcagact tgtctatgat
    23701 gtcgcgtcat actatttttc aacacgttga aatcaggtca gggagagaag tatgaaatta
    23761 ttgcagcggg gcgtggcgtt agcgctgtta accacatttg cactggcgag tgaaactgct
    23821 ctggcgtatg agcaggataa aacctacaaa attacagttc tgcataccaa tgatcatcat
    23881 gggcattttt ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg
    23941 gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact ttccggtggc
    24001 gacattaaca ctggcgtgcc cgagtctgac ttacaggatg ccgaacctga ttttcgcggt
    24061 atgaatctgg tgggctatga cgcgatggcg atcggtaatc atgaatttga taatccgctc
    24121 accgtattac gccagcagga aaagtgggcc aagttcccgt tgctttccgc gaatatctac
    24181 cagaaaagta ccggcgagcg cctgtttaaa ccatgggcgc tgtttaagcg tcaggatctg
    24241 aaaattgccg tcattgggct gacaaccgat gacacagcaa aaattggtaa cccggaatac
    24301 ttcactgatg tcgaatttcg taagcccgcc gatgaagcga agctggtgat tcaggagctg
    24361 caacagacag aaaagccaga cattattatc gcggcgaccc atatggggca ttacgataat
    24421 ggtgagcacg gctctaacgc accgggcgat gtggagatgg cacgcgcgct gcctgccgga
    24481 tcgctggcga tgatcgtcgg tggtcactcg caagatccgg tctgcatggc ggcagaaaac
    24541 aaaaaacagg tcgattacgt gccgggtacg ccatgcaagc cagatcaaca aaacggcatc
    24601 tggattgtgc aggcgcatga gtggggcaaa tacgtgggac gggctgattt tgagtttcgt
    24661 aatggcgaaa tgaaaatggt taactaccag ctgattccgg tgaacctgaa gaagaaagtg
    24721 acctgggaag acgggaaaag cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag
    24781 caaatgatct cgctgttatc accgttccag aacaaaggca aagcgcagct ggaagtgaaa
    24841 ataggcgaaa ccaatggtcg tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc
    24901 aatatggggc ggttgattct ggcagcccaa atggatcgca ctggtgccga ctttgcggtg
    24961 atgagcggag gcggaattcg tgattctatc gaagcaggcg atatcagcta taaaaacgtg
    25021 ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg acatgaccgg taaagaggtg
    25081 attgattacc tgaccgccgt cgtgcagatg aagccagatt caggtgccta cccgcaattt
    25141 gccaacgtta gctttgtggc gaaagacggc aaactgaacg accttaaaat caaaggcgaa
    25201 ccggtcgatc cggcgaaaac ttaccgtatg gcgacattaa acttcaatgc caccggcggt
    25261 gatggatatc cgcgccttga taacaaaccg ggctatgtga ataccggctt tattgatgcc
    25321 gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg
    25381 aaaggtgagg tgagctggca gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc
    25441 ttaatcgcgg cgggcgatat cagcaaattt ggcatcgagg atctttgcca gatcgcctgc
    25501 cgccagttcg atatccagtc cgcgcttgcc gccggaaaca taaatagtgg caaattcttg
    25561 tgcgggggcg tcgataatcg ttggcagacg ttttttctgc cccagtgggc taatcccccc
    25621 aaccaggtat cccgtcgaac gctgcgcgac catcggatcg gccatctcaa ctttcttggc
    25681 acccagcgct tttgctactt ttttaagatc cagttgacct gcgaccggcg taacggccac
    25741 ggcaaggtgt ttcatatcac cgttcactgc caccagcagc gttttgtaga cctgatccgg
    25801 attcaaacct aattttttga cgacttcatc gccaaaattg gtttcagccg gatcgtgctc
    25861 gtaggtatgg atttgaaacg aaatcttgtt tttttcgagt aatttaactg cgggtgtcat
    25921 ggtaattttc ctgccttaaa caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa
    25981 aaaaagagtc atcttgccta agagtattgg caggatggtg agattgagcg acaatcgagt
    26041 tacaccgtcg cttaaagtga cggcataata ataaaaaaat gaaattcctc tttgacgggc
    26101 caatagcgat attggccatt tttttagcgc aacatttgcg gcaaattccc ttctccatac
    26161 aggtgtagtg caccgaccgc gaccacatat cgccccggcg gcatggcgcg taatttatcc
    26221 cgccaggcga gatttcgctg atgcatcagc acatcgtaca gcgactgact gaacgtattg
    26281 ggcagcgtta tatcattatt ttgcggcggt gcattcagcc accagctcat catttgttgc
    26341 agcaaccgtg cgttggtatg ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg
    26401 tcagggagct ggagcaacat ggcaatctgg ttttcagccc cttccagttc aatcacgggt
    26461 ttatgttgtt gcttcgccgc ctgcaatagc tggtaatcga taccgtattc tgcccgcagc
    26521 cccagttttt gtgcctgcgt cgcctgaaga accatcgcga tttgccacag cggttgggta
    26581 gaaaagagtg agggagaaat gcccatctcc tggctaatgt gctgcaggtt ttggagttgc
    26641 tcctcgctaa tgcgctcttc cagcgcctcg caggcaggca aattagcaaa aggcgtatcg
    26701 ctggtggaaa catccgcctc gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa
    26761 cgggtgggca ggggagccat atcgtggcta cccatatgaa tactgccaat cagatgaaaa
    26821 tggcgattgc cggggagggt gatatcgatg gcaggccagg agtaatgatt accgcgcagc
    26881 gcggcccaaa gcgtttttac ccggtacaac agatccatac gacctccctt tgtgaaatat
    26941 catgctagcg cgcggggagg gatggcgcaa atggagttgg ggacttgttt aatgtgtttg
    27001 tataattcag tatgttcttg tatcgctatt cacagggaag caacagttaa aaactatgaa
    27061 acaggcaaca agaaaaccga cgacacccgg agatattctt ctctatgaat atctggaacc
    27121 gctcgatttg aaaatcaatg agttagcaga gttgctgcat gttcatcgta atagcgtcag
    27181 tacactgatc aataataatc gtaaactcac cactgagatg gcattccgtc tggcgaaagt
    27241 ttttgatacc acagtcgatt tttggctaaa cctccaggcg gcagttgatc tttgggaagt
    27301 tgaaaacaac atgcgcaccc aggaagaatt gggacggatt gaaacagtgg ctgaatattt
    27361 ggcacgccgt gaagagcgtg caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg
    27421 taggccggat aaggcgttta cgccgcatcc ggcatccgag caccattgcc tgatgcgacg
    27481 cttacccgtc ttatcaggcc tacaaacctg tgcctgaacc gtaggccgga taaggcgttt
    27541 acgccgcatc cggcagtcat gcgtcgatgc caaatgtgcc accctaaagc agcgcatccg
    27601 caatgacata cttattcctt cggtttaaac cgcagcaacc ggttggcgtt actcactacg
    27661 gtaatcgacg agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagta
    27721 aacggccaca aaataccggc ggcgaccgga ataccgatac tgttgtagat aaaggcaccg
    27781 agcaggttct gcttcatgtt gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg
    27841 cccatcaggc tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg
    27901 ccccccatcg caataccgac atccgcctgg gccagcgctg gcgcatcgtt aatgccgtcg
    27961 cccaccattg ccacctgacg tccttcactt tgcaggcgtt taatcgcttc ggctttaccg
    28021 tccggcagca ccccggcgat cacttcatca atcccggctt ctttggcgat cgcattggcg
    28081 gtggttgggt tatccccggt caacatcacc agacgatatc ccgctttatg caggcgttgc
    28141 agcgccgcca cgctatcact acgcaatgga tcgcgtactg ccagcagggc taccgctttc
    28201 ccgtcaaccg ccagcagcac aggcgttgcc ccttgcgatg cctgagcagt aatctccgct
    28261 tcgatagctt tggtaccaac ctgttgctca tttaacagcg cctgattgcc cagcaataac
    28321 gcatgacctt cagcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc
    28381 tgcggcagct gcatatcacc tgctttatcg aggatcgccc gtgccagcgg atggctggaa
    28441 ccttgctcca gtgccgccgc cagacgcaat gcctgcgctt catcaacatc agcaaacgtt
    28501 ttcactgcga caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacacgacg
    28561 gtatcgagtg tactggcgcg ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca
    28621 gccgcccgcc cgacgccgga aataatcgac atcggcgtcg ccagccccag cgcacacgga
    28681 caggcgataa tcagtaccgt ggtggcaatc accagggtat aaacaatttg cggtgccgga
    28741 ccaaagaagt accagattgc cgcactgacc agcgcaatca ccaccaccac aggtacaaat
    28801 acggctgaga ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc
    28861 accatgcgaa tgatccgtga cagcgtggta tggctgccaa ccgcactggc acgaaacagc
    28921 acgctaccgt cctgtaccac tgtcccggca tggacgcttt cgccttcgcc tttttgctgc
    28981 gggattggtt cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg
    29041 ccatctaccg gcacgcgatc gccggtcgtc aggcgcagca acatacctgg ctgcacttcc
    29101 gccagaggca cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa
    29161 tcgagtaatt tctccagcgc cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg
    29221 ccgagattga tcagaccgat aatcatcgcg ctggcttcgt aataaagatg tcgcgcttcc
    29281 atcgggaacc actgcggcca caggttgacg ctcatcgaat agagccacgc cacgccagta
    29341 cccagcgcca ccagcgtatc catcgtcgct gcaccgttca gcaggctttt ccatgcactg
    29401 cggtaaaaat ggccgccggc gaaaaccatc accgccaggg ttatcaggcc gataaccaac
    29461 cacaggctgc ggttgtcagc ggtgaccatc atgttatcgc caatcatccc ccagaccatc
    29521 accgggatac ccaccgccag tgcgacaatt gcctgccagc ggaagcgctt catcgtagca
    29581 acggcggttt cctgctggcg ctcacggcgt ttcgcgtcat cttcaatcgc ttccgcgccg
    29641 tagcccgctt tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc
    29701 gcagtacgct ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca
    29761 ttttgtacgc gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg
    29821 tcatcgtcat cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac
    29881 gggattgatg actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc
    29941 gcttgtttga tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca
    30001 gtgatagaca catccgcctg ctcaacatcc gggcgctgtt caagactttc tttcacgcgt
    30061 ttaacgcagt gaccgcagga caggccgtcc agggtcaggt cgatagtttg tgacataaaa
    30121 cactccttta agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa
    30181 ggtcaagaaa ttaataaacc aggcgagtaa aagtccgtaa agattaaaaa attggctcaa
    30241 tttgcatcag gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa
    30301 gttaaatcag gatgcctgaa aatcggcacc ggggtgagga attacctccc gcatctataa
    30361 aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt
    30421 acacccaatt tcactcactt aacggcggtc aaaatgccga ctacattccc tttctggcga
    30481 atgtgcctgg tcaactggcg gcagtggcta tcgtgacctg cgatggcaag gtctatagtg
    30541 cgggtgacag tgattaccgc tttgcgctgg aatccatctc gaaagtctgt acgttagccc
    30601 ttgcgttaga ggatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg
    30661 gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaaccg ctttcgccac
    30721 tggtaaatgc tggcgccatt gccaccacca gcctgattaa cgctgaaaat gttgaacaac
    30781 gctggcagcg aattttacat atccaacagc aactggctgg tgagcaggta gcgctctctg
    30841 acgaagtcaa ccagtcggaa caaacaacca acttccataa ccgggccatt gcctggctgc
    30901 tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc
    30961 agtgctccac gctcctcaat actgttgaac tggcaacact tggcgcgacg ctggcggcgg
    31021 ggggagtgaa tccgctgacg cataaacgcg ttcttcaggc cgataacgtg ccgtacattc
    31081 tggccgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggca tatcgcgttg
    31141 gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggtgtgatgg
    31201 gaattgccgc gttctcacca ccgctggacg aagaaggcaa cagtgttcgc ggtcaaaaaa
    31261 tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa
    31321 cacggaaggt aataacggta acaaacccct cggtctatgg aacgtcgttt ccatcggcat
    31381 tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg caggctgcgt tgctaatgga
    31441 agcctcgacc tgggtcgcct ttgcttttgg cggtattgtg gcgatgtttt ccggttatgc
    31501 ctatgcgcgt ctgggggcga gctatcccag caatggcggc attatcgact tctttcgtcg
    31561 cggattaggc aacggcgtct tttcgctggc gctctcgtta ctgtacctgt tgacgctggc
    31621 ggtgagcatc gccatggtcg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga
    31681 aggcagccag gaggagcatc ttattttgct ctacgcgttg gggatcattg cggtgatgac
    31741 gcttttcaac tccttaagca accatgcggt agggcggctg gaagtgatcc tcgtcggcat
    31801 taaaatgatg atcctgttat tgctgattat tgccggtgtc tggtcgttgc aaccggcgca
    31861 tatttctgtc tctgcgcccc ccagctccgg tgcgttcttc tcctgtatcg ggataacctt
    31921 ccttgcctat gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgca
    31981 ggtcattatg ccacgggcgt ttctggtggc aattggcgtt accacgttgc tttatatctc
    32041 gctggcgctg gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgccgatac
    32101 cgccgtagcg caggcagctt ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat
    32161 cggcgcttta ctggcgacgg cttcagccat taacgcgaac ctgttcgccg tgtttaacat
    32221 catggacaac atgggcagcg aacgcgaact accgaagcta atgaataaat ccctgtggcg
    32281 gcagagtacc tggggcaaca tcattgtcgt ggtgttgatt atgctgatga cggcggcact
    32341 gaatttaggc tcactcgcca gcgttgccag cgccaccttt ttgatttgct acctggcggt
    32401 gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt
    32461 tggtacgttg gtgatgttgt tggtgatcgt tggctttatc tacagtctgt ggtcccaggg
    32521 tagccgtgcg ttgatatgga ttattggctc actcttactc agccttattg tggcaatggt
    32581 catgaagcgc aataaaaccg tataacatct ctctgtgcgc agtacttcct gtattattgt
    32641 ggtggcggtc gatattcgca ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt
    32701 aacaaagcac aggaggcgtt gcgcgaacga tgaacatcag cgatgtagca aaaattaccg
    32761 gcctgactag caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc
    32821 gcagcgaaaa cggttatcgc acctacacgc agcagcatct caacgaactg accttactgc
    32881 gccaggcacg gcaggtgggc tttaacctgg aagagagcgg cgagctggtg aatctgttta
    32941 acgacccgca gcggcacagc gccgacgtca aacggcgcac gctggagaag gtggcggaga
    33001 tcgaacgaca cattgaggag ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg
    33061 cctgccctgg cgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc
    33121 atcatcgggc agggtgatta agacgagacg gctcggatat gtagggttat cccttctatc
    33181 gcaatgactt caacatgcgt acctgcaccg agatcctcgc tggcgctgac aggccatgaa
    33241 ctgtcaccga cgcgcatatg accgcgcccg ttgaccagcg gagattccag cacaaaacgt
    33301 cggccaatca gctgctgccc gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc
    33361 acccgccgcg acaaccattt ccaccacagc caggcggcga gcagcgtcag gatggcaaac
    33421 attacccctt gccactccca acccagcggc accagccaga ccaccaggcc ggtaatcacc
    33481 gctgccacgc cgctccacaa caaataacca tttccgccca gcatctcggc tgccagcagc
    33541 aaaccgccga gactgagcca gaaaatatgt ggatgaacga ccattaactc catcatggct
    33601 gagtccgttt gttggcgctg tctttcacca gctcggcaat cccggcaatc gaccccatca
    33661 ggctgctggc ctctaatggc atcatcacta ctttgctgtt actggaggaa ccgatctgct
    33721 gtaacgcttc ggtgtatttc tgcgctacga agtagttcac cgcctgaata tcaccggagg
    33781 cgatagcttc agacaccatt ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg
    33841 cttcagcctg taaaaacgcc gactgacgtt cgccttccgc tttcaggatt tgcgactgtt
    33901 tttcaccttc ggctttgagg atttccgcct gacggatccc ttccgcttca agaatgtaag
    33961 cgcgtttggt acgttccgct ttcatctgcg cgttcattga agagataagc tctgccggtg
    34021 ggcgcacgtc gcgaatttca atacgggtga ctttaatccc ccacgggttg gtggcttcat
    34081 cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt
    34141 ccattgaacc caacacggta cggatgttag tcatggtcag gttgatgatc gccagctcca
    34201 gattgctgac ttcataagcc gcgcgcggcg cgtcaatcac ctgaataaaa cagactgcgt
    34261 cgatggtaac gttggcgtta tctttcgaga taacttcctg ggaagggata tcgagcactt
    34321 gctccatcat attgatcttg cgaccaatgc gatccataaa cggcaccacc agactgagcc
    34381 ccggctgtaa cgttttggta tagcgaccaa aacgttctac cgtccattga tagccctgcg
    34441 gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac aaaaatgaga atcgggataa
    34501 agataagcat cggaaaaacc tcctgttgta ccgtccataa tcagcaaaat tgctgcttga
    34561 ttaaacaaat tatacctgat tactgaaaga gagttccccc ttattcctgc gaaggataaa
    34621 ctgtttttag taaaaatcag aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc
    34681 agctacaaaa cgtaggatat ctggcgggtg atgcgaagat tcttaataac atcaattttt
    34741 cgctgcgtgc tggcgaattt aagttaatta ccggtccttc tggttgtggc aaaagtacgc
    34801 tgctaaaaat agttgcttca ttgatcagcc caaccagcgg aacgttactg tttgaaggtg
    34861 aggatgtcag cacactaaag ccagaaatct accgccaaca agtctcttac tgcgcccaga
    34921 caccgacgct gtttggcgat acggtatacg ataatctgat ctttccctgg cagatccgta
    34981 accggcagcc tgacccagcc atttttctcg attttctcga acgcttcgcc ttgccggaca
    35041 gcattttgac gaaaaatatc gccgagctat ctggtggtga aaaacaacgc atctcactga
    35101 ttcgtaacct gcaatttatg ccgaaggttt tattgctgga tgaaatcacc agtgcgctgg
    35161 atgaaagtaa taaacataac gttaatgaga tgatccatcg ttatgtgcgc gagcaaaata
    35221 ttgccgtgct gtgggtgaca cacgataaag acgaaattaa tcatgcggat aaagtgatta
    35281 cactgcaacc gcatgccgga gaaatgcagg aagcacgcta tgaactcgca taatattact
    35341 aacgaatcat tggcactggc attaatgctg gtggtggtgg caatcttaat tagccataaa
    35401 gaaaaactgg cgctggagaa agatattttc tggagcgtcg ggcgagcgat aattcagctg
    35461 attattgtcg gctatgtgct gaagtatatt ttcagcgtgg atgatgccag cctgacatta
    35521 ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat
    35581 attgctaaag cttttatctc atcgtttatt gccattacgg tcggggcggg aattaccctg
    35641 gcggtgctga ttctctctgg gtcgattgaa tttatcccga tgcaggtgat ccctatcgcc
    35701 gggatgattg ccggtaacgc catggtagcg gtggggttgt gttacaacaa tttagggcaa
    35761 cgggtcatta gcgaacagca acagattcag gagaaactga gtcttggtgc gacgccgaag
    35821 caggcttcag cgatattgat tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat
    35881 tcagcaaaaa cggttggctt agtgagttta cctggaatga tgtccgggct gatatttgcc
    35941 gggattgatc cggtgaaggc gattaaatat cagattatgg tgacctttat gctgctctca
    36001 accgccagct tgtcgaccat tattgcctgc tatttaacct atcgtaagtt ttataattcg
    36061 cgccaccagt tggtggtgac gcaattgaag aagaaatgat gcgatgccgg atgcgcagca
    36121 tcgcatccgg cgttgtggtt catgtgccga tcaacggaat atcaatacaa caatgcatac
    36181 agctggcggc gatacttcga cgccagtgca tcacccgtac ccagcgcagc gaggatctcc
    36241 tggaacgttt tacgcgtctg accgtctgcg gcggtgagat ctttacgcag atgcccgaac
    36301 agcaactcca gcgcctcttc attgcgccca acctggtgca gttgcagagc cagttgcgtc
    36361 gccagtgcgg catcttctgg attctccgcc acctgctgtt gcaactgttg aatttccggc
    36421 gtatcagccg cctgcttcag cagttcgatt tgcgccacca gcccctggta gcgggtgtcc
    36481 tgatcctgca acggaatggt tttcagcacc gcttccgcat cttcagaacg gttcagcgca
    36541 atcagcgttt ctgccagcag caggccgatc tccccgttct gattcgacaa ctgccaggcg
    36601 tctttcagca atggcagggc atcggtgtaa ttgccttcct gcatcagttg catcgcctgc
    36661 tgcgctttca gctcttcttc gcgcggcagc actttatcca gcagggcgcg gatcgcctct
    36721 tccggttgcg gcccctggaa gccatctacc ggttgcccgt tctggaacag atacacggtc
    36781 ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct ccgcgtcgca gtccagcttc
    36841 gccagaataa attgcccgtt gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc
    36901 aaacagtgct ggctacgttc agaccaaaaa tagaacagca ccggagtggt catcgactgt
    36961 tcaagaacct gttgcaggtt agattcgtta atgttgacaa tattttctac ggacatggag
    37021 tcgctctctg ttgtcgatat tttctttgac atgggggctt aagcgcgcgc ttcaactcac
    37081 ccctgcaata ttttgtccat cacgcgcccc ggcagcaggc gcttgagcac cattactgcc
    37141 caggtcacca gcgttaccgg atagcgcatc ttcggcttct cgctaataaa agcatggcgt
    37201 actttgtcca ccaccgcttc cggtcccaac gtaaagcggg cggcgatgcc gggattttcg
    37261 actggtttat cactttgcgt ctggttgacg ttgtcggtga agcgagtacg aatgggaccg
    37321 ggttcgatca ggctgacttt aattccgctg tggcgcagct ccatgcgcag tgcgtctgac
    37381 cacgcctcca gcgcatattt actggccgcg taagcgccac gacccggcgt ggagattaat
    37441 cccatcaccg atgatgtcat cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac
    37501 aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc
    37561 gcacggctga tggtggaaag ggggccatac atgccgaatc cggcattgtt aaagatccca
    37621 tacagacaat tatcggtcag ggcgatcacc tcgtctgctg cgcgatcaac actttctggt
    37681 gaatccagat cgatcaacac gccggtaaat cccatgttgt tcatacgctc aacatcatcc
    37741 ggttttcggc aacctgccag cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc
    37801 aggccaattc cactggaaca tccggtaatt aagaccgatt tttgcataac tttacctgtc
    37861 aggatctccg ttgctttatg agtcatgatt tactaaaggc tgcaactgct tcgccatcca
    37921 gtcggcaata aacggctggg cgtcgcggtt gggatgaata ccgtcatcct gcatccattg
    37981 tggcttgagg tagacctctt ccataaaaaa gggcagcagc ggaacatcaa actctttggc
    38041 gagtttgggg taaatggcgc taaaggcttc attataacgg cgaccatagt ttgcaggcag
    38101 acgtatttgc attaacaatg gttcagcgtt ggcggctttg acatcctgca aaatctggcg
    38161 cagcgtttgc tcggtttgct gtggctgaaa accacgcaaa ccgtcattgc cgcccagttc
    38221 aaccagcacc caacgcggct gatgctgttt cagcagagcc ggaaggcgcg ccagtccttg
    38281 ttgcgaggtg tcgccgctga tgctggcatt aactaccgac gttttactct gccacttatc
    38341 attcaacaag gcaggccagg ccgcgctggc agacattcga tacccggcgc tcaggctatc
    38401 acccagaatc aataacgtgt ccgctgcggc ggcacggaag gttaacagga ccaggaacag
    38461 gaagggcaaa tgccagcgga aaacattgtt gaagttcatc atcttaagaa gtccgtcggt
    38521 cagggggagc atgaactctc catcctcacc ggagttgagc tggttgtcaa acgtggcgag
    38581 accatcgcac tggtgggcga gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc
    38641 gggcttgatg acggcagcag tggcgaagtg agtctggtgg gacaaccgct acataatatg
    38701 gacgaagaag cgcgggcaaa gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt
    38761 atgttaattc ctacccttaa cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt
    38821 gagagtagcg cggaaagtcg taacggggcg aaagcgttgc tcgaacagtt agggctgggt
    38881 aaacgtctgg atcatcttcc ggcacagctt tccggcggtg aacagcaacg agtggcgctg
    38941 gcacgagcct ttaatggtcg acctgatgtg ctgtttgccg acgaacccac cggcaacctt
    39001 gaccgccaga cgggcgataa aattgccgac ctgctgtttt ccctcaaccg tgaacatggc
    39061 accacgttga ttatggtgac ccacgacctg caactggcgg cacgctgcga ccgctgctta
    39121 cggctggtga acgggcagtt gcaggaggaa gcatgattgc acgttggttc tggcgcgaat
    39181 ggcgttcgcc gtcgctatta attgtctggc tggcgctaag cctggcggtg gcctgcgtgc
    39241 tggcgctggg caatatcagc gatcgcatgg agaagggctt aagccagcaa agccgtgagt
    39301 ttatggcggg cgatcgggcg ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg
    39361 aagcgcaaaa gcgcggcctg aaagtcggca agcagctgac tttcgccaca atgacctttg
    39421 caggcgacac accgcagctg gcgaacgtca aagcggtgga tgatatctac ccgatgtatg
    39481 gcgatctgca aactaatccc cctggcctga aaccgcaggc gggcagcgta ttgctggccc
    39541 cacgcctgat ggcactgctt aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca
    39601 ccttgcggat tgccggagaa gtgattcagg aaccggattc cggttttaac cccttccaga
    39661 tggctccgcg tctgatgatg aatctggctg atgtcgataa aaccggagcc gtgcagccgg
    39721 ggagtcgggt cacctggcgt tataaattcg gcggcaacga gaaccagctc gacggctatg
    39781 agaaatggtt gttacctcag cttaaacccg aacaacgctg gtacggtctg gaacaggacg
    39841 aaggcgcgct ggggcgatcg atggaacgct cgcaacagtt cctgctgctt tcggcgcttc
    39901 tgaccttgct gctggcagtg gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc
    39961 gctacgatct ggtggcgatc ctcaaagcgc tgggggcagg gcgagcgcaa ctgcgtaagc
    40021 taatcgtcgg tcagtggttg atggtgctga cgctttcagc cgttaccggt ggggccatag
    40081 gcctgttgtt cgaaaacgtg ttgatggtgc tgctcaagcc cgttctgcct gctgcactac
    40141 cgccagccag cctctggccg tggctgtggg cgcttggcac catgacggtc atctcgctgc
    40201 tggtggggct acgaccatac cgcttgttgc tggcaacgca gcctttacgc gtattacgta
    40261 atgatgtggt agcgaacgtc tggccgctga agttttatct gccgattgtc agtgtggtgg
    40321 ttgtgctgct gctcgccgga ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg
    40381 gcgcggtagt actggctttg ctgtgcggtg tgctgggctg gatgcagctg aatgtacttc
    40441 gccgcatgac gctgaaatcg ctacctctgc gcctggcggt tagccgcctg ttacgtcagc
    40501 cgtggtcaac gttaagccag ctttcggcat tttcgctctc ctttatgctg ctggcgctgt
    40561 tgctggtatt gcgtggcgat ctgctcgacc gctggcagca gcagctaccg ccagaaagcc
    40621 cgaactactt tttaattaac atcgccacag aacaggtagc accgctaaaa gcgttcctcg
    40681 cggaacatca tatagtcccg gaatcatttt atccggtagt gcgggcgcgg ctgacggcga
    40741 ttaacgataa gccgacagaa ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta
    40801 cctggcaaaa tacgcggccc gatcataatc cgattgtcgc cggtaactgg ccgccaaaag
    40861 ccgatgaagt gtcgatggaa gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata
    40921 ccgtgacttt tatgggcgat acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag
    40981 tggactggga aagtctgcgg cctaatttct attttatttt cccggaaggg gcattagacg
    41041 ggcaaccgca gagctggctt accagtttcc gctgggagaa tggcaacggc atgttgacgc
    41101 aactcaaccg ccagttcccg accattagcc tgttagatat tggcgcgatt ttaaaacagg
    41161 tcggtcaggt gctggagcag gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg
    41221 cctgcggtat gttgctgttg ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg
    41281 agctggtggt atggcgcaca ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt
    41341 gtgagttcgc catgcttggg tttgtttccg gcctggtggc cgcaattggt gcggaaacgg
    41401 cactggcagt gttgcaggcg aaagtgtttg atttcccgtg ggagccagac tggcgattgt
    41461 ggattgttct gccgtgcagc ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg
    41521 cgcgactggt taagggtaag gcgctgttca ggcagtttgc ggggtgatga gagtaaataa
    41581 ggggcgttct ggttgaatgg aacgccttgt ttaaattaat tctacaatat gtttcattga
    41641 tgatgttatt gaattggtga tttcctatct ttctattgct gatat
//
LOCUS       DAJSKJ010000014        39350 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000014 DAJSKJ010000000
VERSION     DAJSKJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.014"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            152..1516
                     /locus_tag="ON345_000658"
     CDS             152..1516
                     /locus_tag="ON345_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3070768.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTCQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(1644..2135)
                     /gene="yajQ"
                     /locus_tag="ON345_000659"
     CDS             complement(1644..2135)
                     /gene="yajQ"
                     /locus_tag="ON345_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR3070769.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2303..3214
                     /gene="panE"
                     /locus_tag="ON345_000660"
     CDS             2303..3214
                     /gene="panE"
                     /locus_tag="ON345_000660"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3070770.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3177..3767
                     /gene="yajL"
                     /locus_tag="ON345_000661"
     CDS             3177..3767
                     /gene="yajL"
                     /locus_tag="ON345_000661"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR3070771.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3821..5269)
                     /gene="thiI"
                     /locus_tag="ON345_000662"
     CDS             complement(3821..5269)
                     /gene="thiI"
                     /locus_tag="ON345_000662"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR3070772.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5475..5717
                     /gene="xseB"
                     /locus_tag="ON345_000663"
     CDS             5475..5717
                     /gene="xseB"
                     /locus_tag="ON345_000663"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR3070773.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5717..6616
                     /gene="ispA"
                     /locus_tag="ON345_000664"
     CDS             5717..6616
                     /gene="ispA"
                     /locus_tag="ON345_000664"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR3070774.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALEHIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6641..8503
                     /gene="dxs"
                     /locus_tag="ON345_000665"
     CDS             6641..8503
                     /gene="dxs"
                     /locus_tag="ON345_000665"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR3070775.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8558..9532
                     /gene="yajO"
                     /locus_tag="ON345_000666"
     CDS             8558..9532
                     /gene="yajO"
                     /locus_tag="ON345_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR3070776.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9586..10104)
                     /gene="pgpA"
                     /locus_tag="ON345_000667"
     CDS             complement(9586..10104)
                     /gene="pgpA"
                     /locus_tag="ON345_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR3070777.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10082..11059)
                     /gene="thiL"
                     /locus_tag="ON345_000668"
     CDS             complement(10082..11059)
                     /gene="thiL"
                     /locus_tag="ON345_000668"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR3070778.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11137..11556)
                     /gene="nusB"
                     /locus_tag="ON345_000669"
     CDS             complement(11137..11556)
                     /gene="nusB"
                     /locus_tag="ON345_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR3070779.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11576..12046)
                     /gene="ribE"
                     /locus_tag="ON345_000670"
     CDS             complement(11576..12046)
                     /gene="ribE"
                     /locus_tag="ON345_000670"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR3070780.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12135..13238)
                     /gene="ribD"
                     /locus_tag="ON345_000671"
     CDS             complement(12135..13238)
                     /gene="ribD"
                     /locus_tag="ON345_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR3070781.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13242..13691)
                     /gene="nrdR"
                     /locus_tag="ON345_000672"
     CDS             complement(13242..13691)
                     /gene="nrdR"
                     /locus_tag="ON345_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR3070782.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13842..14381
                     /locus_tag="ON345_000673"
     CDS             13842..14381
                     /locus_tag="ON345_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR3070783.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14680..15564
                     /gene="tsx"
                     /locus_tag="ON345_000674"
     CDS             14680..15564
                     /gene="tsx"
                     /locus_tag="ON345_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR3070784.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15741..16088)
                     /locus_tag="ON345_000675"
     CDS             complement(15741..16088)
                     /locus_tag="ON345_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR3070785.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16217..17188)
                     /gene="secF"
                     /locus_tag="ON345_000676"
     CDS             complement(16217..17188)
                     /gene="secF"
                     /locus_tag="ON345_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR3070786.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17199..19046)
                     /gene="secD"
                     /locus_tag="ON345_000677"
     CDS             complement(17199..19046)
                     /gene="secD"
                     /locus_tag="ON345_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR3070787.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19074..19406)
                     /gene="yajC"
                     /locus_tag="ON345_000678"
     CDS             complement(19074..19406)
                     /gene="yajC"
                     /locus_tag="ON345_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR3070788.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19429..20556)
                     /gene="tgt"
                     /locus_tag="ON345_000679"
     CDS             complement(19429..20556)
                     /gene="tgt"
                     /locus_tag="ON345_000679"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR3070789.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMVGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20612..21682)
                     /gene="queA"
                     /locus_tag="ON345_000680"
     CDS             complement(20612..21682)
                     /gene="queA"
                     /locus_tag="ON345_000680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR3070790.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21775..22356
                     /gene="acpH"
                     /locus_tag="ON345_000681"
     CDS             21775..22356
                     /gene="acpH"
                     /locus_tag="ON345_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR3070791.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22361..24178)
                     /gene="malZ"
                     /locus_tag="ON345_000682"
     CDS             complement(22361..24178)
                     /gene="malZ"
                     /locus_tag="ON345_000682"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR3070792.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEMGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24334..25707)
                     /gene="proY"
                     /locus_tag="ON345_000683"
     CDS             complement(24334..25707)
                     /gene="proY"
                     /locus_tag="ON345_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR3070793.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25783..27102)
                     /gene="brnQ"
                     /locus_tag="ON345_000684"
     CDS             complement(25783..27102)
                     /gene="brnQ"
                     /locus_tag="ON345_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR3070794.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27509..28804)
                     /gene="phoR"
                     /locus_tag="ON345_000685"
     CDS             complement(27509..28804)
                     /gene="phoR"
                     /locus_tag="ON345_000685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR3070795.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28862..29551)
                     /gene="phoB"
                     /locus_tag="ON345_000686"
     CDS             complement(28862..29551)
                     /gene="phoB"
                     /locus_tag="ON345_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR3070796.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29741..30943
                     /gene="sbcD"
                     /locus_tag="ON345_000687"
     CDS             29741..30943
                     /gene="sbcD"
                     /locus_tag="ON345_000687"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR3070797.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGANKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30940..34083
                     /gene="sbcC"
                     /locus_tag="ON345_000688"
     CDS             30940..34083
                     /gene="sbcC"
                     /locus_tag="ON345_000688"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR3070798.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEAESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLARHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34209..35393
                     /gene="araJ"
                     /locus_tag="ON345_000689"
     CDS             34209..35393
                     /gene="araJ"
                     /locus_tag="ON345_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR3070799.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSLYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(35536..36444)
                     /gene="mak"
                     /locus_tag="ON345_000690"
     CDS             complement(35536..36444)
                     /gene="mak"
                     /locus_tag="ON345_000690"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR3070800.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36569..37480
                     /gene="rdgC"
                     /locus_tag="ON345_000691"
     CDS             36569..37480
                     /gene="rdgC"
                     /locus_tag="ON345_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR3070801.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37558..37641)
                     /locus_tag="ON345_000692"
     CDS             complement(37558..37641)
                     /locus_tag="ON345_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070802.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37638..37979)
                     /locus_tag="ON345_000693"
                     /pseudo
     CDS             complement(37638..37979)
                     /locus_tag="ON345_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38127..38411)
                     /gene="ppnP"
                     /locus_tag="ON345_000694"
     CDS             complement(38127..38411)
                     /gene="ppnP"
                     /locus_tag="ON345_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR3070803.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38483..39160)
                     /locus_tag="ON345_000695"
     CDS             complement(38483..39160)
                     /locus_tag="ON345_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR3070804.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
BASE COUNT         9771 a        10560 c         9957 g         9062 t
ORIGIN      
        1 ttttcaacat atagagcatt cattttatga atgcaaaaaa acatacctga cacacaaatt
       61 actctcctct gtaatatttt ctcattaaca ctctgtttac ccaacatgcg ctccgcacta
      121 cactatgtgg gcattttttt aaggggtggt aatgaacgat tataaaatga cgccaggtga
      181 gaggcgcgcg acctggggtt tagggaccgt attctcgttg cgcatgctgg gcatgtttat
      241 ggttctgccg gttctgacca cgtacggcat ggctctgcaa ggtgccagcg aagcattaat
      301 cggtattgcc attggtattt atggtctgac tcaggccgtt tttcagattc cgtttggcct
      361 gctttccgac cgcattggtc gcaaaccatt aattgtcggt gggctggcgg tgtttgccgc
      421 cggtagcgtt atcgctgcgc tttccgactc catctgggga attattctgg gccgggcgct
      481 acaaggctct ggagcaattg ccgccgccgt tatggcgctg ctttccgatc tcacgcgcga
      541 acaaaaccgc accaaagcga tggcgtttat cggcgtgagc tttggcatta cctttgccat
      601 tgcgatggtg cttggcccga tcatcactca caaacttggg ctgcacgcgc tgttctggat
      661 gatcgctatt ctggcaacaa ccggcattgc gttgaccatt tgggttgtgc ccaacagtag
      721 cactcacgta cttaatcgtg agtccggaat ggtgaaaggc agtttcagta aagtgctggc
      781 ggaaccgcgg ctgctgaaac tcaactttgg cattatgtgt ctgcacatgc ttctgatgtc
      841 tacgtttgtt gccctgcccg gacagctagc tgatgcgggg ttcccggcgg ctgaacactg
      901 gaaggtctat ctggcgacga tgttaatcgc ctttggctcg gtcgtgcctt tcattatcta
      961 cgctgaagtt aagcgcaaaa tgaagcaagt ctttgtcttc tgcgtcgggt tgatcgtggt
     1021 tgcggaaatt gtgttgtgga acgcacaaac gcagttctgg caactggtgg tcggcgtgca
     1081 gcttttcttt gtggcgttta atttgatgga agcccttctg ccttcactta tcagtaaaga
     1141 gtcgccagca ggttacaaag gtacggcaat gggtgtttac tccacctgcc agtttcttgg
     1201 cgtggcgatt ggcggttcac tgggcggctg gattgacggc atgtttgacg gtcagggcgt
     1261 atttctcgct ggcgcaatgc tggccgcagt gtggctggca gtcgccagta ccatgaaaga
     1321 accggcgtat gtcagcagtt tgcgcattga aatcccggcg aacattgccg caaacgaggc
     1381 gttaaaagtg cgtttgctag aaactgaagg catcaaagaa gtgttgattg cagaagaaga
     1441 acattcagct tatgtgaaaa tcgacagcaa agtgacgaat cgctttgatg ttgaacaggc
     1501 aattcgccag gcataaaagc gaggcgaaat tgcctgctgc gctacgctca tcaggcctac
     1561 taagctcctg atttacaaac ctttgtaggc caaaccaggc gttcatgccg catctgacat
     1621 gaacaaagcg caggttgtcg cgattaatcg cggaagtttt tgaactggaa cggctgaccc
     1681 agatcgccac cgcgcaccat cgccatgaca gcctgcaaat catcacgaga tttgccagtc
     1741 acgcggatct catcgccctg aatttgcgcc tgcactttca gtttgctgtc tttgatcatc
     1801 ttgacgattt ttttctgggt cgcgctctca ataccctgct tcagtttcgc ctccacaaac
     1861 caagttttac cgctatgaac gatattttcc ggtacatcca gtgaactgcc ttcaatgccg
     1921 cgcttcagca gcttggcacg cagaatatcc agcaactgat tgacctggaa atcggactcg
     1981 ctcaacactt tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc aacgttacgg
     2041 aagtcaaaac gggactccac ttcgcggctc gcgttatcga ccgcgttacg tgcttcctga
     2101 agatcaactt cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat
     2161 gcgaagcata atacccgcaa agttaaataa ccgacagtcc tgcaccaaga cgtatccctt
     2221 aattgtgcat agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag
     2281 tggttgcggg tgaggaggaa caatgaaaat taccgtattg ggatgcggtg ccttagggca
     2341 attatggctt acagcacttt gcaaacaggg tcatgaagtt cagggctggc tgcgcgtacc
     2401 gcaaccttat tgtagtgtga atctggttga gacagatggt tcgatattta acgaatcgct
     2461 gaccgccaac gatcccgatt ttctcgccac cagcgatctg ctcctggtga cgctgaaagc
     2521 atggcaggtt tccgatgccg tcaaaagcct cgcgtccaca ctgcctgtaa ccacgccaat
     2581 actgttaatt cacaacggca tgggcaccat cgaagagttg caaaatattc agcagccatt
     2641 actgatgggc accaccaccc atgccgcccg ccgcgacggc aatgtcatta ttcatgtggc
     2701 aaacggtatc acgcatattg gtccggcacg gcaacaggat ggcgattaca gttatctggc
     2761 ggatatttta caaaccgtgt tgcctgacgt tgcctggcat aacaatattc gcgccgagct
     2821 gtggcgcaag ctggcagtca actgcgtgat taatccactg accgccatct ggaattgccc
     2881 gaatggtgaa ttacgtcatc atccgcaaga aattatgcag atatgcgaag aagtcgcggc
     2941 agtgatcgag cgcgaagggc atcatacttc agcagaagat ttgcgtgatt acgtgatgca
     3001 ggtgattgat gccacagcgg aaaatatctc gtcgatgttg caggatatcc gcgcgctgcg
     3061 ccacaccgaa atcgactata tcaatggttt tctcttacgc cgcgcccgcg cgcatgggat
     3121 tgccgtaccg gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga
     3181 gcgcatcggc actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacta
     3241 tcgatctgct ggttcgcggc ggtatcaaag tcaccactgc cagcgtcgcc agcgatggca
     3301 acctggccat tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgagg
     3361 tggctgatgg cgaatatgac gtgatcgtac tgcctggtgg cattaaaggc gcagagtgtt
     3421 ttcgcgatag taccctgctg gttgaaaccg ttaaacagtt ccaccgttcc gggcgtatcg
     3481 tcgcggctat ttgcgccgcg ccagccaccg tgctggtacc gcacgatatc ttcccgattg
     3541 gcaatatgac cggcttcccg acgctgaaag acaaaattcc cgccgaacaa tggcaggaca
     3601 agcgcgtcgt ctgggatgca cgggtaaaat tgctgaccag ccaggggccg ggtacggcta
     3661 ttgattttgg tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc catgaagtgg
     3721 catcacaact ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggc
     3781 aaacgtcgca tctgaccaga tgcgacgtaa aaaccacaaa ttatgggcga tacaccttca
     3841 cattgttaaa gccctgctcg cgcagataga gcgcctgcag acggctcatc accccgcgct
     3901 cacaccacag tagccaggtt ctgttctggt cgagatcgcc aaatttggtg ctcagtttat
     3961 agaacggcag agaaaccacg tcgatacctt caaccttcag cggcttatct tcctgctcat
     4021 cgacagaacg gatatcgagg atcacgtcgt tcgggccaaa gccattgacg gtttccactt
     4081 ccaccacttc ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcttcct
     4141 caaccacttt atcgagaata ctgaagtcga acttctcttc ttccgcttca atcttcgatt
     4201 taaccgcttt caccgtcggg cttttggaga tcacgccaca atattctggc atcgtgcgag
     4261 caaagtcttc agtgccaatc tggcgggcca ggttgatgat gtgctctttg tcgtaagaaa
     4321 tcagcggacg caggatcagc gtgtcagaga cgttatcaat caggcgcagg ttggtcagcg
     4381 tctgactgga cacctggccg agcgcttcgc cggtgaccag cgcctgtacg ccgtaacgtt
     4441 cagccacttt agacgcggca cgcaccatca tacgtttgag gataacgccc atctgaccgt
     4501 cgtcgatttt ctcgagaatt tccccaacga ccggttcgaa attaatagcg acaaaacgca
     4561 cgcggtggga gctgccaaaa cggttccaca gataatgcgc cacctgacgc acgccaattt
     4621 catgcgccgc gccgccaagg ttaaagaaac agtaatgcac gcggcagccg cgacgcatca
     4681 acatataact ggaaacaccg gagtcgaaac caccggaaat gagcgacagc acatcttcct
     4741 gggtgccgat cgggaaaccg ccaatacctt cgtagcggcc tttaatcagc aggagacgat
     4801 cgtcttccac ttccagatgg acagtcactt ccggattggt cagcttcacg cgcgcggact
     4861 caatatgctg atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat
     4921 gtttgccacg gcgcttcacg cgtacgcaga aggttttgcc ttccagctga tcgcgatact
     4981 gaaccaacgc tttctcaaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga
     5041 gaatatggtg gatacccgga atacgggtca gggcgtcgcg aatagccaga cgctggtttt
     5101 catcttttgc gcgaacttcg atgttatccc agtggcggac gacagcgagc gtctcatcat
     5161 agtgctttaa aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag
     5221 attggctttt gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg
     5281 ttcgttggca agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc
     5341 tgcatccagg cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat
     5401 tgcgcgttat ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga
     5461 gtcagacatt cattatgccg aagaaaaatg aggctcccgc cagctttgaa aaggcgctga
     5521 gcgagctgga acagattgta acccgtctgg aaagtggcga cctgccgctg gaagaggcgc
     5581 tgaacgagtt cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag
     5641 ccgaacagcg cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta
     5701 caccggacaa tgagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa
     5761 ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac
     5821 catgcagtat ggcgcattat taggtggtaa gcgcctgcga ccttttctgg tttatgccac
     5881 cggtcatatg ttcggcgtta gcacaaacac gctggacgca cccgctgccg ccgtagagtg
     5941 tatccacgct tactcattaa ttcatgatga tttaccggcg atggatgatg acgatctgcg
     6001 tcgcggtttg ccgacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggcga
     6061 cgctttacaa acgctggcgt tctcgattct aagcgatgcc aatatgccgg aagtgtcgga
     6121 tcgcgacaga atttcgatga tttctgaact ggcgagcgcc agcggtattg ccggaatgtg
     6181 cggtggtcag gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga
     6241 gcatattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt
     6301 aagcgccgga gataaagggc gtcgtgctct gccagtactc gacaagtacg cagagagcat
     6361 cggccttgcc ttccaggttc aggatgacat cctggatgtg gtaggagata ctgcaacgtt
     6421 gggaaaacgc cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg
     6481 tcttgagcaa gcccggaaga aagcccggga tctcatcgac gatgcccgtc agtcgctgaa
     6541 acaactggct gaacagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat
     6601 ccagcgtaat aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa
     6661 atacccgacc ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag
     6721 tttaccgaaa ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag
     6781 cgggcacttc gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta
     6841 caacaccccg tttgaccaat tgatttggga tgtggggcat caggcttatc cgcataaaat
     6901 tttgaccgga cgccgcgaca aaatcggcac catccgtcag aaaggcggtc tgcatccgtt
     6961 cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat
     7021 cagtgccgga attggtattg cggttgctgc cgagaaagaa ggcaaaaatc gccgcaccgt
     7081 ctgtgtcatt ggcgatggcg cgattaccgc tggcatggcg tttgaagcga tgaatcacgc
     7141 gggcgatatc cgtcctgata tgctggtggt cctcaacgac aatgaaatgt cgatttccga
     7201 aaatgtcggc gcgctcaata accatctggc acagctgctt tccggtaagc tttactcttc
     7261 gctgcgcgaa ggcgggaaaa aagttttctc tggcgttccg cccattaaag agctgctcaa
     7321 acgtaccgaa gaacatatta aaggcatggt agtgcctggc acgttgtttg aagagctggg
     7381 ctttaactac atcggcccgg ttgacggtca cgatgtgctg gggcttatca ccacgctgaa
     7441 gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg
     7501 ttatgaaccg gcagaaaaag accccatcac tttccacgcc gtgcctaaat ttgatccctc
     7561 cagcggttgt ttgccgaaaa gtagcggcgg tttgccgagc tattcaaaaa tctttggcga
     7621 ctggttgtgc gaaacggcag cgaaagacaa caagctgatg gcgattactc cggcgatgcg
     7681 tgaaggttcc ggcatggtcg agttttcacg taaattcccg gatcgttact tcgacgtggc
     7741 aatcgccgag caacacgcgg tgacctttgc cgccggtctg gcgattggtg ggtacaaacc
     7801 cattgtcgcg atttactcca ctttcctgca acgcgcctat gatcaggtgc tgcatgacgt
     7861 ggcgattcaa aagctcccgg tcctgttcgc catcgaccgc gcgggcattg ttggtgctga
     7921 cggtcaaacc catcagggcg cttttgacct ctcttacctg cgctgcatac cggaaatggt
     7981 cattatgacc ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta
     8041 taacgatggc ccgtcagcgg tgcgctaccc gcgtggcaac gcggtcggcg tggaactgac
     8101 gccgctggaa aaactaccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggc
     8161 gatccttaac tttggtacgc tgatgccaga agcggcgaaa gtcgccgaat cgctgaacgc
     8221 cacgctggtc gatatgcgtt ttgtgaaacc gcttgatgaa gcgttaattc tggaaatggc
     8281 cgccagtcat gaagcactgg ttaccgtaga agaaaacgcc attatgggcg gtgcaggcag
     8341 cggagtgaac gaagtgctaa tggcccatcg taaaccagtc cccgtgctga acattggcct
     8401 gcctgacttc tttattccgc aaggaacgca ggaagaaatg cgcgccgaac tcggcctcga
     8461 tgccgccggt atggaagcca aaatcaaggc ctggctggca taatccctac tccactcctg
     8521 ctatgcttaa gaaattattc atggcaggag tggcagcatg caatacaacc ccttaggaaa
     8581 aaccgacctt cgcgtttccc gactttgcct cggctgtatg acctttggcg agccagatcg
     8641 tggtaatcac gcatggacac tgccggaaga gagcagccgt cccataatta aacgcgcact
     8701 ggaaggcggc ataaatttct ttgataccgc caatagctat tccgatggta gcagcgaaga
     8761 gatcgttggt cgcgcactgc gggatttcgc ccgtcgtgaa gacgtggtcg ttgccaccaa
     8821 agtgttccat cgcgttggtg atttaccgga aggattatcc cgtgcacaaa ttttgcgctc
     8881 tatcgacgac agcctgcgcc gtctcggcat ggattatgtc gatatcctgc aaattcatcg
     8941 ctgggattac aacacgccga tcgaagagac gctggaagcc ctgaacgacg tggtaaaagc
     9001 cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg ctcaggccct
     9061 gaagctacaa aaacagcacg gctgggcgca gtttatcagt atgcaggatc actacaatct
     9121 gatttatcgc gaagaagagc gcgagatgct gcctctgtgt tatcaggagg gcgtggcggt
     9181 gattccgtgg agcccgctgg cgcgggggcg actgacgcgt ccgtggggag aaactaccgc
     9241 acgactggtg tctgatgagg tggggaaaaa tctctataaa gaaagcgatg aaaatgacgc
     9301 gcagatcgca gagcggttaa cgggcgtcag tgaagaactc ggtgcaacac gagcacaagt
     9361 tgcgctggcc tggttgttga gtaaaccggg cattgccgca ccgattatcg gtacatcgcg
     9421 ggaagaacag cttgatgagc tactgaacgc ggtggatatc actttgaagc cagaacagat
     9481 tgccgaactg gaaacgccgt ataaaccgca tgcggtcgta ggatttaaat aagagtccag
     9541 gcctgatgag acgtgacaag cgtcacatca ggcatcggtg cacaactacg acagaatacc
     9601 cagcggccag tgatgaccga taaagtacag gatgcctgcg gaaatcaccc cggcgacaat
     9661 atcgtcgatc atgatcccca tgccgccatg tacattgcga tcaaaccagc ggatcggcca
     9721 cggcttccac atatccagaa tacggaaaat cacaaacccg gcggcaaccc actgccagtc
     9781 attggtcggc agcgccatga gcgtgatcca cataccaata aattcgtccc agacaatgct
     9841 gccgtgatcg tgcacgccca tatctttcgc cgtttgatga caaagataga cgccgataca
     9901 gatccccaac atcaccacca gcgagtagag ctgccagggt aaaaaggtca tcagatacca
     9961 gaacggaatc gctgccagcg atcccatcgt gccaggaacg attgggctta atccacttcc
    10021 gaatccgaca gcaagcagat gccacggatt actcatcttc aggcgacttt tcgcgacatc
    10081 tttatggcgt ggcaaaatgg tcatatcctt tccagtcaaa cgtgacaggt tcgccgtcac
    10141 gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta aacggtacgc
    10201 caaggtgtcc gagagcaaca tccagcgcgc cacggttcag ttccggcacc gtgaaacaca
    10261 actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt tcaacatggc
    10321 gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg cagtcgctgg
    10381 ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata gctgaatttg
    10441 ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga tggacggaga tgacgtttga
    10501 tcaagtagtc agcatcttta gcatcggcaa cctgcaaacg gttttgcaaa atcgccagcc
    10561 cggcggcgct atcgcccggt gtaccggtca catagatcca gtcacccggt ttcgccccag
    10621 agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc attgataatg
    10681 gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta ataattgaga agatcaaaca
    10741 aactgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt aatgccagcg
    10801 tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc gccagtgctt
    10861 tatacgccag atcagcagga tcgatatcag ggaggaaatg gttacccgcc accagcgtat
    10921 cagtgctgat cgccagggtc tgtttctcgg ggatattgag aagagcgcaa tcgtcgccga
    10981 tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg gcaatcaggg
    11041 agaactcgcc acatgccata cgttatgcct cagcaggtaa aaaagaaaag gccggcgatc
    11101 atggaacggt cttccgtgaa tctaccggcc tggatatcac tttttgttag ggcgaatcac
    11161 aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg cgccgaacga
    11221 tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcgc tacgtttaga
    11281 cagttcgtac agcgcaatgc gcagtactgc tttttctacc tgtcccagtt cttccagcag
    11341 gcgggacagg tatggcttca tcagtccgtc gaggtatgcg gtattagtcg ccaccccggc
    11401 cagcagctca cggaagtaca ggacgtcaac gtcttttaca tcctgttcag ccaggaactg
    11461 gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg cctggacggc
    11521 acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac taatttcagg
    11581 ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct tctgcacctt
    11641 tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca gtggtcagaa
    11701 ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg ttgcttgcac
    11761 caccagcgac atattcaaag tgggcagtgc caccacgaat aaccgtaccc agcgcaatca
    11821 ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc tcataggcac
    11881 caggcaccca aacaacggta atgttttcat cttttacctg accgatacgt ttcagtgcgt
    11941 caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg atggtgatgg
    12001 cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc ttccttcggg
    12061 ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg ctgcttccct
    12121 ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc tacatgacgt
    12181 atctctttga atttaaattg gggggcgtcg gctaatttct caagccctgg cagcgtgcat
    12241 aatccgcggg cgtcgctgcc taatagttta ggtgcgatat agacaatcag ctcatcgact
    12301 aaacccgcct gcagcaatgc gccagcgagc gttggccccg cttccaccca gatgctgtta
    12361 atttgctgtt tacccagttg catcatcagt acaaccagat ccagatgacc tttatgctct
    12421 ggaatcagca aggtacgcac cgtttccggc cactcactag aatcttcctg cgtacgcgcg
    12481 aaccaggttt cgccgggctg ctgcacaatg cgatgttccg gcgtcacgcg attttggcta
    12541 tcaatcacaa tacgtactgg ctggcggaga ttttgttgcg ggtagagcgc ctgagtttgt
    12601 tcatccagtt cagaccaacg cactgttaag gctggatcat ccgccagcac cgtggcgctg
    12661 ctggttaaaa tggcatgact ttgtgcgcgc tgtcgttgta cgtcgcgccg cgcctgaggc
    12721 gaagtgatcc actggctttc gccgctcgcc atcgccgtgc ggccatcaag cgatgcgcca
    12781 agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc tttattcaat
    12841 tgctcggctt cactcatcat cagcccgtgg ctgacgtcaa tgccagcctg ttgcagacgg
    12901 taaagtccac gcccagcgac ctgcgggtta ggatcttgca tcgaggcaac cacgcgcgct
    12961 acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac gaccatgatg gctacagggt
    13021 tcgagtgtga cataggcggt cgcacctttg gctttttcac ccgccatacg caacgcgtgt
    13081 acttcggcat gtggttcacc cgcacgttgg tggtaacctt caccgacaat ttcgccatct
    13141 ttgacaatga cgcacccgac attcgggttg ggatgcgtgg taaaacgtcc tcgttgcgcc
    13201 agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc caggcgcgcg
    13261 atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc aaaacggata
    13321 taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat catcttgctc
    13381 ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttaa tatgattgat tgccatttcg
    13441 acgtcatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct acgcaatttc
    13501 tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat aaccagctcc
    13561 gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg gcggcgtacg
    13621 gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc gaaacagaat
    13681 gggcaatgca tacggcgtcc tgaccaggtg gttaacagaa atctatttta ccctgaactg
    13741 acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttcg ctgcttttac
    13801 cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatact aacgtttttc
    13861 gactgctcct gctgggaagc ctgttcagcc ttagcgcctg tgtgcagcag agcgaagtgc
    13921 gacagatgaa acacagcgtc agcacgctga accaggagat gacgcaactc aataaagaaa
    13981 cggtcaaaat cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc
    14041 tgcctggagc gaaaacaccg gcaagactgg aaagccagat cggtacttta cgtatgtcgc
    14101 tggtgaatat tacgcctgat gcagatggca ccaccctgac gctacgtatt cagggcgagt
    14161 cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa
    14221 tagacaactt tcaggaaatc aatgtgcaga atcaattgat taatgcccct gccagcgtcc
    14281 tcgcccccag cgatgttgat attccgttac agttaaaggg tatttctgta gaacagttag
    14341 acttcgtacg tatccacgat attcaacctg ttatgcagta aacgtatttc ggggcgattt
    14401 tgtgcgtccc gcaacatctt tccccgtcat tttgttactc cgcttacatc acccagattg
    14461 atagtaaaag tttgcaacaa gggtgaaagt cagtacaatc cctgcccgaa tgtgtgtaaa
    14521 cgtgaacgca atcgattacg taaatgatag aactgtgaaa cgaaacatat ttttgtgaac
    14581 aatgattttt ataataggct cctctgtata cgaaatattt agaaacgcaa tttgcgcctt
    14641 tttcactccc gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac attactggca
    14701 gccggtgcgg tactggcgct ctcttcgtct tttactgtca acgcagctga aaacgacaaa
    14761 ccgcagtatc tttccgactg gtggcaccag agcgttaacg ttgtcggaag ctatcacacc
    14821 cgtttcggac cgcagatccg caacgatacc taccttgagt acgaagcatt cgctaaaaaa
    14881 gactggttcg acttctatgg ttatgcggat gcgccggtat tcttcggcgg taactccgat
    14941 gctaaaggta tctggaacca cggttctccg ctgtttatgg aaatcgaacc acgtttctcc
    15001 atcgacaagc tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg gtacttcgcg
    15061 aacaactaca tttacgacat gggtcgtaat aaagatggtc gccagagcac ctggtacatg
    15121 ggtctgggta ccgacatcga tactggtctg ccgatgagcc tgtccatgaa cgtctatgcg
    15181 aaataccagt ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc
    15241 aaaattaaat actttgtgcc gattaccgat ctgtggggcg gtcagctgag ctacatcggc
    15301 ttcaccaact tcgactgggg ttccgattta ggggatgaca gcggtaacgc aatcaacggt
    15361 attaagaccc gtaccaataa ctctatcgct tccagccata ttctggctct gaactacgat
    15421 cactggcact actctgtcgt agctcgttac tggcacgacg gtggtcagtg gaacgacgat
    15481 gcagaactga acttcggcaa cggcaacttc aacgttcgct ctaccggctg gggtggttac
    15541 ctggtagtag gttacaactt ctgattatga aaatgccggg atttattccc ggcatttcag
    15601 attgttaaca acgtgcgctt tgttcatgcc ggatgcggcg caaacgcttt gtccgaccca
    15661 caaaagcacg taaattcaat atattgctga aataacgtag gcctgataag cgtagcgcat
    15721 caggcaattt tacgttttaa tcacttcttc ttgttcatca tcgctttcag gtcagcgaat
    15781 gggttgtact tcgcttcacc gacatctttc tgcgcatctt cccctgcgat aacggtcgta
    15841 ccatactgat ccgcttcggt atatttcgaa tgctcatgat cgtggcaata gagacaaagc
    15901 aattcccagt tactgccatc ttccgggtta ttggtatggt cgtgatcaat gtggtgaaca
    15961 gtaagttcac gcaggttgga ataaacaaac tcgcgggaac agcgaccgca gacccacgga
    16021 tagattttta atgctttttc gcgatagccg ctttctaacc gcgcatagtt ttttgggatg
    16081 atagccatga agagttacct ggatgaaatg tagggttacg cgcagtttcc caagatgcgg
    16141 ggattttcgc aatctccata catcaaaaaa aatcccgatc ttctgaccgg gattttcaac
    16201 atcaacggga acttgattac ggcagaattg acggctgatc cgccccttct ttttccactt
    16261 tctgctgcaa catgtgttcg cgcttcatac ccagtttcag agccaacgca gatgccacat
    16321 agatggaaga tgcagtaccg atggaaacac cgataagcat ggtcagcgag aagccttcca
    16381 gtaccggacc accgaagagg tacagcatca ggataaccat caaggtagta ccggatgtga
    16441 tcaaggtacg gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa ggcgtaccgc
    16501 gacggatctt gcggaagttt tcacgaatac ggtccgatac cacgatactg tcgttaagcg
    16561 agtaaccgat aaccgacatc aacgatgcca caatggtcag gtcaatctcg atatggaata
    16621 acgacaaaat acccagcgta atgataacgt cgtgcgccag cgcaataaca acccctgccg
    16681 ccagtcgcca ctcaaagcga aaacctacgt acacaaggat agacagcagc gccgccatca
    16741 acgccatcgc accggtttgc gcaaggtctg cccccacgct cggaccgacg aactcaatac
    16801 gcttcactgc tgcattctga ttggtggatt cgttaatcac cttcagaacc tggctgccca
    16861 gcacctgacc gccggtttcg ccttcagcag gcggcatacg gaccatgatg tcgtggctgc
    16921 taccaaagtt ttgcagcatt ggctcttcaa aaccggcttt ttgcaatgca tcacgcatta
    16981 cgtcaatttc agccggtttt tcgagcgtaa tttcgataac cgtaccaccg gtgaaatcca
    17041 gcccccagtt aaagccgcgc acgcccataa taacgatagc ggcgattaac agcagaccag
    17101 agatgccgaa agcccagtag tcccagcgca taaagtcata gactttacgg ccgtggttta
    17161 gttgttcaac agtatattcc tgtgccacat cgcactcctc agattgacag cttcttgacg
    17221 cgcttgccgc catatagcag gtttacgatg gcacgcgtac cgacaatcgc ggtaaacatc
    17281 gacgtcgcca caccgatacc ggtagtaatc gcgaaccctt taattgcccc ggtacccact
    17341 gcgtacagga tgatgacttt aatcagcgtg gtgatgttcg catcgaagat agaactgaat
    17401 gcgccacgat aaccttcatc aattgcctgt tgaacagtac gtccgttgct caactcttct
    17461 ttaatacgtt cgttgatcag tacgttcgca tcgaccgcca ccgcaagggt taagacgata
    17521 cccgcgatac ctggcatact cagcgttgcg cctggcaaca gcgacataat gccgacgatt
    17581 aagatcaagt tggcaatcag agcactggtc gcaatcagac caaacttctt atagaagatg
    17641 atcatgaaca gaatagaaac cagcagaccg gcaaggcaag cctccagccc ctgttcaatg
    17701 ttctgcatac ccagggttgg gccaatggta cgttcttcaa caatctgaat cggcgcgatc
    17761 aacgcaccgg cacgcagcag cagtgacagc tgacgggctt cgttcgggtt gttgatgccg
    17821 gtgatacgga agctgttacc cagacgagac tggatgttgg cgatgttaat cacctcttcc
    17881 tgtttcacca gaaccgcacg accatttgca tctttcttac cgctgtcttt gtactccaca
    17941 aacagggttg ccatcggttt gccgatgttg tccttagtga agttagacat gatgttacca
    18001 ccagcgctat cgagcgagat gttaacctgc ggttggttgt attcgtcctg gctggaagtg
    18061 gagtcggtga tatggtcacc ggtcagaatt acgcgtttgt acagcacaac tggctgacct
    18121 tcgcgggtct gtttcacttc agagtcgccc ggtacgcgac cggatgccgc agcggcctgg
    18181 tcaacgttgg tgtttaccag acggaattcc agcgttgccg tcgcacccag aatctctttc
    18241 gcacgcgcag tgtcctgaat acctggcagt tcaacaacga tacggtcagc accctgacgc
    18301 tgaaccaccg gctccgccac gccaagttgg tttacacggt tacgcaggat attgatgttc
    18361 tgctgcaccg catattcacg cgcttcactc agacgagcat cgctcattac cgcacgcagc
    18421 tggttgctgc cctggctact aatcaccagg tccggatggc gcttgctcag atacgcaatg
    18481 gcttcatcac gagctttagc atcgcggaaa gtaatgctca ggccgtagtt gttttcttta
    18541 cgaacggtgg tatacgggat gcccttttca cgcagatcgc tgcgcaggct atcgatattt
    18601 tgttcctgga gtttgccaag cgcggtgtcc atatccactt ccatcaggaa gtgaacgccg
    18661 ccacgcaggt caaggccgag cttcatcggc tcagcgtgaa tagctgccag ccagcgcggc
    18721 gttgccgggg caaggttaag cgccacgacg tatttgtcac ccataacgcc cattaatgct
    18781 tcacgagcgc gcagctgggt gtcagtggag tcgaagcgcg caagaatagc gccctcttcc
    18841 agtgccacag acttagcagt tattttttct tcttgtaacg ttttctggac ctggatcagc
    18901 gtttgctcac tggcggcgac tccgcgcgca ccagtgatct gaacagccgg atcctcacca
    18961 aacaggttgg gaagcgcata cagcagaccg atgacaatca ccacgatcag catgacgtac
    19021 ttccacaaag gataacggtt taacacggca attcccttag ggaaaaattt taattacagc
    19081 gccttcatgg tgcctttcgg caggacggca gctacgaagt cacgtttaat aactacttca
    19141 gtggtgtcat tcagcgcgat agcaatgtag ccgttttccg ctactttggt tacgcgacca
    19201 accaggccac cgttcgtcag aacttcatca cctttggcaa tggagtccat cagctttttg
    19261 tgttctttgg tgcgcttctg ctgtggacgc aggatcatga aatagaaaat cagaccgaat
    19321 accaccagca tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc cgttgccgct
    19381 accgcatcag aaataaaaaa gctcattaaa tttccctcat tattaatatt aatcaacgtt
    19441 caaaggtggt acttctcgcc cctgacgctg gtaaaaatca gttacgaagc tctctaattt
    19501 accctcttca atagccttgc gtaaacccac catcaaacgc tggtagtaac gaaggttatg
    19561 aatggtgttg agtcgcgcgc ctaatatttc gttgcaacgg tcaagatgat gcaagtaagc
    19621 gcgtgaataa ttgcgacagg tgtagcaatc acactcagga tcgagtgggc cagtatcgct
    19681 cttatacttc gcattgcgga ttttcaccac gccatcggtc acgaacaaat gaccatttcg
    19741 ggcgttgcgg gttggcatta cgcagtcaaa catatcgata ccacgacgta cgccttcaac
    19801 caggtcttct ggtttaccaa cgcccatcag gtaacgcggt ttgtctgccg gaatttgcgg
    19861 gcatacatgc tccagaatgc ggtgcatatc tgctttcggc tcacccacag ccagaccgcc
    19921 gacagcgtag ccatcaaaac cgatatctac cagaccttta acagaaatat cacgtaaatc
    19981 ttcgtaaacg ctgccctgaa tgataccaaa cagcgcattt ttgtttccga gactgtcaaa
    20041 acgctcacgg ctacgcttcg cccaacgcag agacatctcc atggagcgtt ttgcgtaatc
    20101 ccagtcagca ggatacggcg tacactcatc aaagatcatg acgatatccg aaccaagatc
    20161 gtactgaatc tccattgatt tttcaggatc gaggaaaatc ggatcgccgt tgatcgggtt
    20221 acggaagtgc acgccctgtt cggtgatttt acgaatatcg ccaaggctga agacctggaa
    20281 gccgccggag tcggtgagga tcggcccctt ccactgcata aaatcgtgca gatcgccgtg
    20341 cagtttcatg atttcctggc ccgggcgcag ccacaggtgg aaggtgttgc cgaggataat
    20401 ttgcgcgcca gtggcttcaa cttcttccgg cgtcatccct tttacggtgc cgtaggtgcc
    20461 aacaggcata aaacaaggcg tttccactac gccacgatca aagaccaggc ggccacggcg
    20521 tgcgcgaccg tcggtggtgt ccagttcaaa tttcattttt tctccactac gtcagaaaaa
    20581 cagtccaacg ttttaaacca gcgccgcgga attactcccc gacgcgctca ttaattgcct
    20641 gcggattgta cgtgataaac atcgcatcac cgtaactaaa aaagcgatat ttctcttcta
    20701 ccgctgcttt ataggcgttc atggtgtgtt gataaccggc aaaggccgaa accagcataa
    20761 tcagcgtcga ctctggcaag tggaagtttg tcaccagcgc atcgaccact ttgtactgga
    20821 agcccggata gataaagatt tgggtatcgt cgaagaacgg ttcaatgaga tcgtttttcg
    20881 ctgcctgagc cgcgctttcc agcgaacgta ctgaagtggt gccaaccgca atcacccggt
    20941 taccgcgcgc tttcgccgcc agtaccgcgt ctaccacatc ctgcggtact tcagcgtatt
    21001 ccgagtgcat gatgtgatct tcaatggtgt cgacgcgcac cggctggaag gtgcccgcac
    21061 caacgtgcaa cgtcacaaac gccatctcca cgcctttggc gcgcaatttt tccagcaaag
    21121 gctcgtcaaa atgcagacca gcggtcgggg ctgcaaccgc gcccggtttt tcgctataaa
    21181 cggtttgata aagttcgcgg tcagcgtctt cgtccggacg gtcgatatac ggcggcagcg
    21241 gcatatggcc gatactgttg aggatatcca gtaccgaacg atcatcattg aattcgactt
    21301 caaacagtgc gccgtggcgc gcggtcattg ttgcgttaat actttcgtca tcgcccagca
    21361 gcagttctgc gccaggtttt ggcgctttcg aggcgcgaat atgcgcaaga atgcgtttgt
    21421 cgtcgagcat ccgctcaacc agcacttcaa tcttgccgcc gctggcttta cgcccaaaca
    21481 ggcgcgccgg gatcacgcgg gtattattaa aaaccagaag atcgccgggg ttgagcttat
    21541 caagtaaatc ggtgaaagta ccgtgcgtca gcgcgcccgt cggcccgtcc agcgacagta
    21601 aacgacagct actgcgttca ggcatgggat agtgggcaat cagggattcg ggcaattcaa
    21661 aggagaaatc ggtaacgcgc atgacactga ctcagactaa aataagaggc gggtagtcta
    21721 gtgccggggc gctctccctg caacctttac ccccttttgg atagaatata atcgatgaat
    21781 tttttagctc acctgcattt agcccatctc gcggaaagct cgctttccgg caatttactg
    21841 gctgatttcg tacgcggaaa tcctgaagaa agttttccgc ccgacgtcgt ggctggcatt
    21901 catatgcatc gacgtatcga cgtattaacc gacaatctgc cggaagtccg cgaagcacgg
    21961 gaatggtttc gtagtgaaac tcgccgcgtt gcgcctatta cgctggatgt catgtgggat
    22021 cactttcttt cccgccactg gtcgcagctg tcgccggact ttccgctaca ggaatttgtc
    22081 tgttatgccc gcgagcaagt gatgacgatt ttgccggact caccgccacg ttttatcaat
    22141 ctgaacaatt acttgtggtc agagcagtgg ctggtgcgct atcgcgatat ggatttcatc
    22201 cagaacgtgt taaacggcat ggcaagccgc cgcccacgtc tggatgccct gcgtgactcc
    22261 tggtacgatt tagacgctca ttatgacgcc ctcgaaaccc gcttctggca gttttatccg
    22321 cggatgatgg cgcaggcgtc acacaaggcg ttataaaacg ttagttcatc cataccgtag
    22381 ccgaaatggc aggcaaagcc agaatcccgt cagtcagttg cccatgccct tctttgcatt
    22441 gccattgcac ggcattgaga aacggtgacg cgggtagcac cacttcacag gcctcgccac
    22501 ggttgattgc caccagtaca cgttgctgat tcagcacgcg gacaaatacc actacgttat
    22561 cttccgcata cagcacctga cagccgccac gacgcagcgc ctgacttttc ttacgcagca
    22621 caatcattcg ccggtacagc gcgaataacg ccgtatcctg cttttccacc tgccagggga
    22681 acggtttacg gcaaaacgga tcgtttttac catccagtcc catttcatca ccgtaataaa
    22741 tgcacggtac accaggccag gtgaacagcc agaccaccgc cagcggcagg cgcgcaatat
    22801 cccgaccgag cagcgtttta aatcgcgcag tatcgtggct gtcgagctga ttaaacatac
    22861 gtaattgttg ttgatgagaa agccctgcgc ggtaattatc catccaggcc atacaggttt
    22921 gggcatcaat ttgctgcgga tcgtaagaga tatcggtatt ggcaagaaat ccccacaacg
    22981 ggaatgtgaa gccacgatag ttcatggcgg catcttccac atcggcctgt aaccattgcc
    23041 gtgcatcgcc aaaatgttcg ccgacaatat acgcttccgg ctgggtttct ttcgccgctt
    23101 cggtgatccc agcaacgtgc tgcatattat tgcgcgcccc acccgcctcc cccagcatat
    23161 gcaccacatc cagccgccag ccgtccatac tccacggcgc tttcagccaa tggcggacaa
    23221 tactgtcttc cccgcgataa atttcattca ccagactttc cgactgataa tccagcttcg
    23281 gcaagctggc atagccaagc cagtcgagcg ccgtgccatc atcactaaac gagtaccagt
    23341 cgcgccaggg cgattcaggg ttgtgacaag ctccccccgt gccacgattg tgcctgtcaa
    23401 accaggcatg ggaatcgcca ctgtggttaa acacgccgtc cagcaccagc cgcattccca
    23461 gctgctgcgt attgtgtcgc aaacgcagca acgccccatc accgccaaac tgcggatcga
    23521 catggcgata atcctcggta tcgtatttat gtacgctggg agctttaaac accggattga
    23581 gatacagcgc tgtcacgcca agctttttca gatacggcag tttttcgctt atcccgtcca
    23641 gatcgccgcc atagaacgtt gatccgcccg cctgcgccgt gaccggttca tcccagtcac
    23701 gcaagatgat ctcttgtccg gctgcatgat ggtaatagac atgatcctgt tcagcttcac
    23761 gaggaagact acgcgcaaaa cgatcaggga agatctgata aaaaatctga tccgcagccc
    23821 attgtgggcc gatatccggt acatcgacgg caaactgctc cagtcgtgcc ggcggcattc
    23881 ggctgaagcc ctgcggtgta aaccaacgct ggcgatcgtg ccacagcagt ttgaaactgt
    23941 aacgccgccg gggttgtccg ctggagagat caatcgccgc acgccatgcg gtgacgccag
    24001 gctgctgctg actgcgctgc ttatgcatcg atactgacat ttcttcgtta tcgtgttctg
    24061 tacgcagcat aatgcgctgc ggtgggtctt cgcccgtcag ccacagtgtt atgagcagtt
    24121 gatctttgct ttgtttaaca aatgggggca ccggcaggtg ccatgcattt aacatcataa
    24181 ttcccctgtg atgaaaatct gatcaccttg ccatagccta atgcaggatc actcatcatc
    24241 tgaaggatat ttgggggagg aggacggggg cggataactt tcggaatagc cggatcagga
    24301 tttcctcctg aaccggctta cagaacgcaa gtattattgg ttttcagcca gctgacgatc
    24361 gtggcgacgc ttaaacatcc agccaatcaa cagcacaaca atccacgcga aaccgacata
    24421 cagcgagata cgcgtatccg ggtgataacc aatcaacccg ataataaaga gcaggaaaat
    24481 cagcccgccg atggtcgttg ctaccccacc cggcacttta aatttcagcg ccttaacttc
    24541 ttctggcggc aaacggcgac ggaaggcgat ttgcgacagc aggatcataa tccacaccca
    24601 taccgtggcg aaggttgcca gcgaagcgat caccaggaag acgttttccg gcatgatgta
    24661 gttcagatac accgcaaaca gcagcgcggt agtcatcacc agcaccgtaa cccacggaat
    24721 accgcgacgc gacgttttgc tgaaaatttt cggcgcgctg ccctgctctg ccataccgtg
    24781 gagcatacgg cctacgccaa atacatcact gttaattgcc gacagcgaag cagtcagcac
    24841 aacaaagtta agaatgctgg cggcaaaggt aatgcccata tgctggaacg tcagcacgaa
    24901 cgggctaccg gcagtgccaa cctgattcca cgggtagata gacataatga cgaacagcgt
    24961 accgacgtag aacaccagaa tacgcatcgg cacggagtta atcgcacgcg gtatcgattt
    25021 ctcaggatct ttcgcttcac cggcggtaat cccgataatt tcgatcccac cgtaagcaaa
    25081 catcaccatt tgcaacgaca ttaccatgcc aagccagccg ttactgaaga agccgccgtt
    25141 gctccacagg ttatgaatac cggtcggttg cccgccgttg ccaatccccc agatgatgat
    25201 gccgaaaccg gcgacaatca tgatgatgat ggtggcgact ttaaagaacg agaaccagaa
    25261 ttccagctca ccgaatacct tcacgctcat caggtttacg gcgcaaatga tcagcaccac
    25321 gctcagtacc caaatccagt gcggcaccgt cgggaaccag acacccatat agataccaaa
    25381 agcggtcaca tcggcgatgg cgacaataag gatttcaaag cagtaggtcc agccggtaat
    25441 gtaacctgcc agcgggccga ggttttcctg cgcataacgc gagaaagagc tggcggccgg
    25501 gttatgtacc gacatttccc ccagcgcacg cataatgata tacgccgcga taccaccgat
    25561 aatataggcc aacaacacgc tcggaccggc cattttgatg gcgtctgccg aaccgtaaaa
    25621 cagcccggtg ccaattgctg aacccagtgc cataaagcgt atgtggcggg tacttagccc
    25681 acgctttagc ttgttcttac tttccatcaa ttcctaaccc atcaacacaa taaaaaacca
    25741 cggggcatcg cagccccgtg gttaaaacaa atgttcagtg atttagtgag cgctggaggt
    25801 cacctgacga cctgccgcgc gatcccagat aatggccaga accaccatca ccactgttgg
    25861 cattaaccac gccagacctt gttcggccag cggtaaacgc tgcgcccagg acggtaagat
    25921 atcgctgaat gcagatgctt tgatcccgtc gagaatacca aaaagcaggc tgataaacat
    25981 cggcggagca atcacgcggg acgaattatg ccaccatgag cgtgtaaaac ttaatacaac
    26041 cagtgcgata cacggcggat aaatagcggt cagcaccggt acggagatct ggatcagctg
    26101 gcttaagccg aggttagaaa ccaccatcga gaagccgccg aggataaaca ccagcgtacg
    26161 ataagagagc ggtacgtact gggcaaagaa ttctgcacaa gcacaggtca ggccaactgc
    26221 cgttaccagg caggcgatga agattaacgc cgccagcagg aagctaccgc cgccgccaaa
    26281 ggtgtgctga acgtaagcat gcagaatagc agcgccgttt gcagactgat cgaccagcga
    26341 cgcgctgtct gaacccagac ggaacagcgc caggtacagc agagtcagac caacacccgc
    26401 catcaggcca gcccagacgg tataacgggt cagtaggcgc gcttcggtaa cgccacgaga
    26461 acgcgccgcg ttaacaataa cgataccaaa caccattgcg cccagcgtat ccatggtcag
    26521 atagccgtta acgaaaccgt tagaaaacgc agcgttttga taagcctcag tcgccgtgct
    26581 gatagaaccc gccggccaga caatcgcggc aacagacagg atgaccagcg cgataatttt
    26641 cagcggcgca aggaagttgc ccacggtatc cagcagcttg cccggataga gcgaaaccag
    26701 aataacgata gcgaaataga ccaggctgta gataaacagc ggcagcgcgg aatcacccgt
    26761 cagcggcgca atccccactt caaaggaaac ggtagctgta cgcggcgtag cgaaaagcgg
    26821 ccccaccgcc aggtaacaaa ccgttgccag cagtacgcca gcgactttac cgattggcgt
    26881 gctgaggctg tcaacaccgc cgccaacttt tgccagcgcc actaccgtta gtaccggcag
    26941 accaacggca gtaatgagga agccgaatgc cgcagtccag acgtgttcgc ctgcctgcaa
    27001 gccgaccatt ggagggaaaa taatgttacc tgcgccgacg aacaacgcaa atgtcataaa
    27061 gcccagagcg atgatatcgc gcgatcttaa ttgatgggtc ataaatcata ctgcctgtgg
    27121 atgtggtgtt gtaacgttaa atttttgtgc caatccctgg ggatgataca gcggtatatt
    27181 taaacaattt tagcgggaaa tactcccaac caggcgcggc gaagaatagt ttttcgattt
    27241 accgcgttaa tgcagtcagt atgacagcac ctgaagcgca atttaaacgc ttataacgtt
    27301 ttaaagcaag gagggatcta aaaaccagag gtatatagcg ataagaaatc accaaccagt
    27361 ataaaacact atttccgtaa aaaagacatg gctaatcatg cgaacaaaaa atcaccaacc
    27421 gttgtaaaat ctaacgcccg tattttgact gcaaagaaaa taagccagca tcgactggct
    27481 tatggcaata aaagatgaca aaggcggatt aatcgctgtt tttggcaatt aaacgttccg
    27541 ggataacaaa actgaaacgt gttccttttc ctactgtact ctcaatattc aggcgacttt
    27601 cgtgatgatt cacagcatgt ttcacgatcg ctaaccctaa tccgctaccg ccggtttgcc
    27661 gggaacgcgc tttatcaacg cgataaaaac gctcggtcag gcgcggaata tgctccggtg
    27721 caatgcccgg tccgttatct tcaacgctaa attcggcacc gtgcggcact cgctgccagc
    27781 gtacggtgat atgcgtgcct tccggcgtat gattcacggc gttatagacc aggttcgaaa
    27841 tcgcactgcg tagctgatct tcgttgccag acaccttgag gccgttatct atctcaaagg
    27901 taaatgtctg ttttttctga ctcagagtct gagcctcgcg ctcaacaacg cgcagcatca
    27961 tcggcacatc aaccttttca ttgagcaaat gcgtcggtgc ggcttctatt ttcgacagcg
    28021 tcagcaattg cttcaccagt ccttccatcc gctgggtctg ctcgcgcatg gtgtgcaacg
    28081 ctttttcgcg taccgcgcct tccagcggct gctcattcat catctccagg taaccctgta
    28141 acacggtcaa tggcgtacgt aactcatggc tcacgttggc aaaaaagtta cgccgcgccc
    28201 cttccagttg atgcatttgc gtgacatcac gcgccaccat cagcaactgt ttgtgggtat
    28261 aaggcatgac gcgaatttcc agatgccgcc cggtgttgag caccagattg agcgggcgag
    28321 aaaaatcacg cgttttcaga tattgcgtaa actccgggta acgcagtagg ttaaggatgt
    28381 tctgcccgtt atcttccggc cagcgcaaac caagaatttg ttgcgccaga ccgttacacc
    28441 agaaaatacc gccctcttcc gtggtcagca ccaccgcgtc gggcagcgac tccgcgccgc
    28501 tacgaaagcg tttaatcaga ttgcccagtt cacggcggcg ttttttattt cgcagctgca
    28561 tctggtgtaa gccgtatagt agcggttccc agctaccacg ccccggtggc ggggtcatac
    28621 tgcgatccac ccacagccac catgaaaggc gcaataaatt ccagaaatgc cagataagca
    28681 gtcctgttac cgatgccagc aaaaaccagg gcaggtaacc aaaaaatgca cccaggatga
    28741 aagccgggag gcagcaaagt agcagctcca gcaccagcct tttccacgac agccgttcca
    28801 gcacgtaaga tactccagtt aagaaatcat aagccctgct ctgcgtccga tgagcaaggc
    28861 gttaaaagcg ggttgaaaaa cgatatcctg taccgcgcac ggtctgcacc atgcggtcat
    28921 gcccgccggg ctccagtgct ttacgcaggc gacgaatgtg gacatcgacc gtgcggtctt
    28981 ccacatacac gttagttccc cagacgtggt ttaacagctg ctcgcggctg tacacgcgct
    29041 caggatgcgt cataaaaaag tgcagcagtt taaattctgt cggccccatc tccagcggct
    29101 cttcgcccgc catcactcgg tgagatgtcg ggtcgagact taatccctgc atctcaatca
    29161 cctcttccac cgccattggc gaaatacggc gcattaccgc tttgattcgc gccaccagct
    29221 ccttcggcga aaacggcttg gtgatatagt catccgcgcc ggtttcaagg ccgcgcacgc
    29281 gatcttcttc ttcccctctg gcggtcaaca tcaccactgg aatatcccgg gtcatcgact
    29341 cgcgcttgag gtgtttgatg aactggatac cggagccgcc aggtaacatc cagtcaagga
    29401 gaattaaatc cggccagggt tcattcagtt gattcacagc actgtcataa tcttccgctt
    29461 cgaccggctg aaagccattt tgttcgagca cgaagcagac catttcgcga attggagctt
    29521 catcttctac gaccagaata cgtctcgcca tgatttgccc tgttgtaata aataggttgc
    29581 gatcattaat gcgacgtcat tatgcgtcag atttatgaca gatttatgaa aagctcgtcg
    29641 cacatatctt caggttattg atttccgtgg cgcagaaaaa agcaaatggc acatctgttt
    29701 gggtataatc gcgcccatgc tttttcgcca gggaaccgtt atgcgcatcc ttcacacctc
    29761 agactggcat ctcggccaga acttctacag taaaagccgc gaagctgaac atcaggcttt
    29821 tcttgactgg ctgctggaga cagcacaaac ccatcaggtg gatgcgatta ttgttgctgg
    29881 tgatgttttc gataccggct cgccgcccag ttacgcccgc acgttataca accgttttgt
    29941 tgtcaattta cagcaaactg gctgtcatct ggtggtactg gcaggaaacc atgactcggt
    30001 cgccacgctg aatgaatcgc gcgatatcat ggcgttcctc aatactaccg tggtcgccag
    30061 cgccggacat gcgccgcaaa tcttgcctcg tcgcgacggg acgccaggcg cagtgctgtg
    30121 ccccataccg tttttacgcc cgcgtgacat tattaccagc caggcggggc ttaacggtat
    30181 tgaaaaacag cagcatttac tggcagcgat taccgattat taccaacaac actatgccga
    30241 tgcctgcaaa ctgcgcggcg atcagcctct gcccatcatc gccacgggac atttaacgac
    30301 cgtgggggcc aataaaagtg acgccgtgcg tgacatttat attggcacgc tggacgcgtt
    30361 tccggcacaa aactttccac cagccgacta catcgcgctc gggcatattc accgcgcaca
    30421 gattattggc ggcatggaac atgttcgcta ttgcggctcc cccattccac tgagttttga
    30481 tgaatgcggt aagagtaaat atgtccatct ggtgacattt tcaaacggca aattagagag
    30541 cgtggaaaac ctgaacgtac cggtaacgca acccatggca gtgctgaaag gcgatctggc
    30601 gtcgattacc gcacagctgg aacagtggcg cgatgtatcg caggagccac ctgtctggct
    30661 ggatatcgaa atcactactg atgagtatct gcatgatatt cagcgcaaaa tccaggcatt
    30721 aaccgaatca ttgcctgtcg aagtattgct ggtacgtcgg agtcgtgaac agcgcgagcg
    30781 tgtgttagcc agccaacagc gtgaaaccct cagcgaactc agcgtcgaag aggtgttcaa
    30841 tcgccgtctg gcactggaag aactggatga atcgcagcag caacgtctgc agcatctttt
    30901 caccacgacg ttgcataccc tcgccggaga acacgaagca tgaaaattct cagcctgcgc
    30961 ctgaaaaacc tgaactcatt aaaaggcgaa tggaagattg atttcacccg cgagccgttc
    31021 gccagcaacg ggctgtttgc tattaccggc ccaacaggtg cggggaaaac caccctgctg
    31081 gacgccattt gtctggcgct gtatcacgaa actccgcgtc tctctaacgt ttcacaatcg
    31141 caaaatgatc tcatgacccg cgataccgcc gaatgtctgg cggaggtgga gtttgaagtg
    31201 aaaggtgaag cgtaccgtgc attctggagc cagaatcggg cgcgtaacca acccgacggt
    31261 aatttgcagg tgccacgcgt agagctggcg cgctgcgccg acggcaaaat tctcgccgac
    31321 aaagtgaaag ataagctgga actgacagcg acgttaaccg ggctggatta cgggcgcttc
    31381 acccgttcga tgctgctttc gcaggggcaa tttgctgcct tcctgaatgc caaacccaaa
    31441 gaacgcgcgg aattgctcga ggagttaacc ggcactgaaa tctacgggca aatctcggcg
    31501 atggtttttg agcagcacaa atcggcccgc acagagctgg agaagctgca agcgcaggcc
    31561 agcggcgtcg cgttgctcac gccggaacaa gtgcaatcgc tgacagcgag tttgcaggta
    31621 cttactgacg aagaaaaaca gttacttacc gcgcagcagc aagaacaaca atcgctaaac
    31681 tggttaacgc gtcaggacga attgcagcaa gaagccagcc gccgtcagca ggccttgcaa
    31741 caggcgttag ccgaagaaga aaaagcgcaa cctcaactgg cggcgcttag tctggcacaa
    31801 ccggcacgaa atcttcgtcc acactgggaa cgcatcgcag aacacagcgc ggcgctggcg
    31861 catattcgcc agcagattga agaagtaaat actcgcttac agagcacaat ggcgcttcgc
    31921 gcgagcattc gccaccacgc ggcgaagcag tcagcagaat tacagcagca gcaacaaagc
    31981 ctgaatacct ggttacagga acacgaccgc ttccgtcagt ggaacaacga actggcgggt
    32041 tggcgtgcgc agttctccca acaaaccagc gatcgcgagc atctgcggca atggcagcaa
    32101 cagttaaccc atgctgagca aaaacttaat acgcttgcgg cgatcacgtt gacgttaacc
    32161 gccgatgaag ttgctagcgc cctggcgcaa catgccgagc aacgcccact gcgtcagcgc
    32221 ctggtcgcgc tgcatgggca gattgttccc caacaaaaac gtctggcgca gttacaggtc
    32281 gctatccaga atgtcacgca agaacagacg caacgtaacg ccgcacttaa cgaaatgcgc
    32341 cagcgttata aagaaaagac gcagcaactt gccgatgtga aaaccatttg cgagcaggaa
    32401 gcgcgcatca aaacgctgga agcccagcgt gcacagttac aggcgggtca gccttgtcca
    32461 ctttgtggtt ccaccagcca cccggcggtc gaggcgtatc aggcgctgga gcctggtgtt
    32521 aatcagtctc gattactggc gctggaaaac gaagttaaaa agctcggcga agaaggtgcg
    32581 gcgctgcgcg ggcaactgga tgcattaacg aagcagcttc agcgcgatga aaacgaagcg
    32641 caaagcctcc gacaagatga gcaagcactt actcaacaat ggcaagccgt cacggccagc
    32701 ctcaatatca ccttgcagcc acaggacgat attcaaccgt ggctggatgc acaagatgag
    32761 cacgaacgcc agctgcggtt actcagccaa cgacatgaat tacaagggca gattgccgcg
    32821 cataatcagc aaattatcca gtatcaacag caaattgaac aacgccagca gcagctctta
    32881 acggcattgg cgggttatgc actgacattg ccacaggaag atgaagcaga gagctggttg
    32941 gcgacacgtc agcaagaagc gcagagctgg cagcaacgcc agaacgaatt aaccgcgctg
    33001 caaaaccgta ttcagcagct gacgccgatt ctggaaacgt tgccgcaaag tgatgatctc
    33061 ccgcacagcg aagaaactgt ggcgctggat aactggcggc aggttcatga acaatgtctc
    33121 gcattacaca gccagcagca gacgttacag caacaggatg ttctggcggc gcaaagtctg
    33181 caaaaagccc aggcgcagtt tgacaccgcg ctacaggcca gcgtctttga cgatcagcag
    33241 gcgttccttg cggcgctaat ggatgaacaa acactaacgc agctggaaca gctcaagcag
    33301 aatctggaaa accagcgccg tcaggcgcaa acgctggtca ctcagacagc agaaacgctg
    33361 gcacggcatc aacaacaccg acctgacggg ttggctctca ctgtgacggt ggagcagatt
    33421 cagcaagagt tagcgcaaac tcaccaaaag ttgcgtgaaa acaccacgag tcaaggcgag
    33481 attcgccagc agctgaagca ggatgcagat aaccgtcagc aacaacaaac cttaatgcag
    33541 caaattgctc aaatgacgca gcaggttgag gactggggat atctgaattc gctaataggt
    33601 tccaaagagg gcgataaatt ccgcaagttt gcccaggggc tgacgctgga taatttagtc
    33661 catctcgcta atcagcaact tacccggctg cacgggcgct atctgttaca gcgcaaagcc
    33721 agcgaggcgc tggaagtcga ggttgttgat acctggcagg cagatgcggt acgcgatacc
    33781 cgtacccttt ccggcggcga aagtttcctc gttagtctgg cgctggcgct ggcgctttcg
    33841 gatctggtca gccataaaac acgtattgac tcgctgttcc ttgatgaagg ttttggcacg
    33901 ctggatagcg aaacgctgga taccgccctt gatgcgctgg atgccctgaa cgccagtggc
    33961 aaaaccatcg gtgtgattag ccacgtagaa gcgatgaaag agcgtattcc ggtgcagatc
    34021 aaagtgaaaa agatcaacgg cctgggctac agcaaactgg aaagtacgtt tgcagtgaaa
    34081 taactattca gcaggataat gaatacagag gggcgaatta tctcttggcc ttgctggtcg
    34141 ttatcctgca agctatcact ttattggcta cggtgattgg tagccgttct ggtggttgtg
    34201 atggtggtat gaaaaaagtc attttatctt tggctctggg cacgtttggt ttggggatgg
    34261 ccgaatttgg cattatgggc gtgctcacgg agctggcgca taacgtagga atttcgattc
    34321 ctgctgccgg gcatatgatc tcgtattatg cactgggggt ggtggtcggt gcgccaatca
    34381 tcgcactctt ttccagcctc tactcactca aacatatctt gttgtttctg gtggcgttgt
    34441 gcgtcattgg caacgccatg ttcacgctct cttcgtctta cctgatgctc gccattggtc
    34501 ggctggtatc cggctttccg catggcgcat tttttggcgt cggagcgatc gtgttatcaa
    34561 aaattatcaa acccggaaaa gtcaccgccg ccgtggcggg gatggtttcc gggatgacag
    34621 tcgccaattt gctgggcatt ccgctgggaa cgtatttaag tcaggaattt agctggcgtt
    34681 acaccttttt attgatcgct gtttttaata ttgcggtgat ggcatcggtc tatttttggg
    34741 tgccagatat tcgcgacgag gcgaaaggaa agctgcgcga acaatttcac tttttgcgca
    34801 gcccggcccc gtggttaatt ttcgccgcca cgatgtttgg caacgcaggt gtgtttgcct
    34861 ggttcagcta cgtaaagcca tacatgatgt ttatttccgg tttttcggaa acggcgatga
    34921 cctttattat gatgttagtt gggctaggga tggtgctggg aaatatgcta agtggcagga
    34981 tttcaggacg ttattcacca ctgcgcattg cagcagtgac tgactttatc attgtactgg
    35041 cactgctgat gctctttttc tgcggcggca tgaaaacaac gtcgcttatt tttgctttta
    35101 tttgttgcgc gggattattt gccctttcag cacctctgca aatattgtta ctgcaaaatg
    35161 ccaaaggcgg agagttatta ggtgccgcag gtgggcaaat agcgtttaac ctcggtagcg
    35221 ccgtcggcgc atattgcgga ggtatgatgc tgacgctggg gctggcatat aattacgtgg
    35281 cgctgcctgc cgccctgctt tcgtttgctg cgatgtcatc gttgctgctg tatggtcgct
    35341 ataagcgcca gcaagcggcg gatagttcgg tgctggcgaa accactgggg tagattactg
    35401 tcacggtgcg cgttgcttat tgccggatgc ggcgtgaacg ccttatccgc cctacgcggt
    35461 tctggcacat tttgcaggcc tgataagacg cggcaagcgt cgcatcaggc atgatgcgcc
    35521 aattgcctac gttttttact cttgtggcca taaccacgca gcgccgcgta cgccgctgga
    35581 atcaccgtgc ttcgccttac gcaccggcgt ttcacattcg ccgccgaaga caaattgttt
    35641 aatcaactgc ggaaccgttt gatataaacg gtctacattg ctcatcccac cccccaggac
    35701 aatcacatcc ggatcgagaa tattcacgac atgtgccagc gattttgcca gccgcagctc
    35761 gtagcgacgc aatgccagtt ccgctaccgg atcgctttct tcaaccaggc ggataatttc
    35821 actgcctttc agcgcatgtc cgctcaaacg acgataatcc gtcgcgaatc ccgtgcccga
    35881 aataaaggtt tcaatacaac cttgtttacc gcaataacaa gggacttcct cgcgataacg
    35941 cagttcgtct tcgtccatcc acggtagcgg attgtgtccc cactcacctg ccgtgccatt
    36001 gccgccgata tgcgcccgcc cattgaatgc cacgcccgcg ccgcatcccg tgccgataat
    36061 cacggcaaat actgtctgcg ctcccgctgc cgcgccatct actgcttctg aaaccgccag
    36121 acagttggcg tcatttgcca gccgcacttc ccgctgcaac ctcgcgctta agtctttatc
    36181 gaatggctga ccgttgagcc aggttgaatt ggcattctta accacaccgg tgtaaggcga
    36241 aattgagcca ggaatgccca tacctaccgt tccgcgctgc cctgtcgcct gctccgccat
    36301 atcaaccaac gtggcgatcg tttcaatagt ctgccggtaa tcatcacgcg gcgtgggcag
    36361 acgatggcgg tacaactgct cccctgcatc gcccagtgca atcacttcag ttttggtgcc
    36421 gcctaaatcg atacctatac gcacggtact ctccttattt ttttcaatat caatagcgta
    36481 gagacggaca accggattgg caatgcaagg ccgccgacaa ttcgttatca tgcccgctaa
    36541 atttaacgac aaggccgtgg aaattatcat gctgtggttc aaaaatttaa tggtttaccg
    36601 tcttagccgc gagatttcgc tgcgtgcaga agagatggaa aaacagctag cctcgatggc
    36661 atttacccca tgcggcagcc aggacatggc gaagatgggc tgggttcctc cgatgggatc
    36721 gcacagcgat gcgttaacgc acgttgccaa tggtcaaatt gttatctgcg cgcgcaaaga
    36781 agaaaaaatc ctcccgtctc cggtgattaa acaggcgctg gaagcgaaaa tcgccaaact
    36841 ggaagcggaa caggcgcgta agctgaagaa aaccgaaaaa gattcgctga aagacgaagt
    36901 gctacactct ctgctgccgc gtgctttcag ccgttttagc cagacaatga tgtggatcga
    36961 cacggttaac ggtttgatta tggtggactg cgccagtgcc aaaaaagcgg aagatacgct
    37021 ggcattactg cgtaaaagcc tggggtcgtt accggttgta ccattgagca tggaaaaccc
    37081 gattgaactg acgctgaccg aatgggttcg ctccggtagt gcggcacagg gcttccagct
    37141 gcttgatgaa gccgagctga aatcgttgct ggaagatggc ggcgtgatcc gcgcgaagaa
    37201 acaagatctg accagcgaag agatcaccaa tcacattgaa gccggaaaag tggtgactaa
    37261 actggcgctc gactggcagc agcgcattca gtttgtgatg tgcgacgatg gttcgcttaa
    37321 gcgtctgaag ttctgcgacg agctgcgcga tcaaaacgaa gatatcgacc gtgaagattt
    37381 cgcccagcgt tttgatgccg atttcatcct gatgactggt gaactggcag cgttaattca
    37441 aaacctgatt gaaggattag gtggcgaagc acaacgttaa ttgctgattt tcctttaatg
    37501 ccggatgcga cgcctgccgc gtcttatccg gcgtacgaag ccacaccagg catataatta
    37561 ttcgctacgg cgggcaataa tttttagcgc agcaatatta tgcgttttat gctgtaactt
    37621 gctccatgga cgttgtgtca ttgtttttcc tcaagccagt gacgcgcttc actgcgaatt
    37681 ttccgcattg ccccggtttt cactaaatcc cgtaacagcg aaagatcggg ttgttcgaca
    37741 aataagagta atgatggatc ctcttttaac cacgtcgttt tcacatcggc agcgagctgt
    37801 ggattattca tagctagcga ccgatgccga gactcggtca atgtcgttaa caacgacgcc
    37861 ggggtatgtg cgttgcgtaa tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat
    37921 gcacgccacc attcatcatc tgatttatct tttatcgtag ccagttccgc aacaatatct
    37981 ggagatcttc actgatattt atatcaacct tgatgaatta tgaatatatc ggaaattgaa
    38041 aaaatcgcag caaaggaaat attattatgg gttaaagaga ggatatttca gaaaaatgcc
    38101 ctcccgttca aggggaaggc gaggaattac agatagcggc acagataaga ggtgggttcg
    38161 gcaacttgca gatgaaactc actgtgaccg ggaacattaa acaccgaacc ggcttcatac
    38221 acctgccagt cggtcgcgtc aggcagtaac acattcagcg cgccactgat taccgtcatc
    38281 tcttccggct cagcggtgct gaaggtgtat tcgccttcaa ccataacacc cacgctggcg
    38341 cgaccagtgc tgctgctgga aaagccgatt gatttcactt tgccggaaaa gtactcatta
    38401 ctttgaagca taaactggcc ctgtctggta gttgaacatc tacttcacta taggggccag
    38461 aggcgctggc tgtcacgcaa aattacacca gtaattccgc agccagccgt gcaatcaata
    38521 cgttagacag caagacagga acatcgagct gtttttgcag taaatcgcga tgacgttggt
    38581 gaaatcctaa acaatccagc atgatgacat cagccccttt tgccagtaat tctttcccgg
    38641 catcaatgat tttttgttct gaatcatgaa tggggttacc caatgaaaat accggcgatt
    38701 tctgcaaaat ttgccatttt tgcgcctgaa cggtcagcat ctcctcaacc ggaacgataa
    38761 cccccacctg atgatcttca acaatagagg aaaccagtgg aggcaatatt cgcgacggct
    38821 caagaaagat cgtattacgc gcagtcatac tactaatgtt tgctgtactc attaataaaa
    38881 tgacgtcata accctgatta tcgagcactt caaccacacc ttgcaggtct cgctccactt
    38941 tacgacgcga aacatgggcc agctggttgt catttaataa tgtgagaatg gtgtcttcgc
    39001 ctgcttctgg cgcgtactcc gccatcactt cttcacgact taacttcccc agcaggctat
    39061 gatgggaaat attatcttcg tcaatgtatt ccgtcaggag cggcaaaacc tcctgcatag
    39121 gtacaatgcc gatggtcagg atcgccaacg acgcactcat tgttcatcca ccttactttt
    39181 ctttcgtcgt taattaccgg gcaagtgtga agcaccactg ctgacactta cttgtgctac
    39241 aaatgacaaa aagcgtagca gcagacgtgc acggcataat gtaaagattc caaatgattc
    39301 cagtaatgga ttgttattgt ttaatattct aattattaga aaaacatgaa
//
LOCUS       DAJSKJ010000015        38921 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000015 DAJSKJ010000000
VERSION     DAJSKJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.015"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..707)
                     /locus_tag="ON345_000696"
     CDS             complement(<1..707)
                     /locus_tag="ON345_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3070805.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARIAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMQF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLL"
     gene            complement(830..1282)
                     /gene="tabA"
                     /locus_tag="ON345_000697"
     CDS             complement(830..1282)
                     /gene="tabA"
                     /locus_tag="ON345_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR3070806.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1427..2020)
                     /locus_tag="ON345_000698"
     CDS             complement(1427..2020)
                     /locus_tag="ON345_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3070807.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2091..2804
                     /gene="bdcA"
                     /locus_tag="ON345_000699"
     CDS             2091..2804
                     /gene="bdcA"
                     /locus_tag="ON345_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3070808.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2935..3330
                     /locus_tag="ON345_000700"
     CDS             2935..3330
                     /locus_tag="ON345_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR3070809.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            3611..3745
                     /gene="pyrL"
                     /locus_tag="ON345_000701"
     CDS             3611..3745
                     /gene="pyrL"
                     /locus_tag="ON345_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR3070810.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3749..4684
                     /gene="pyrB"
                     /locus_tag="ON345_000702"
     CDS             3749..4684
                     /gene="pyrB"
                     /locus_tag="ON345_000702"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR3070811.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4697..5158
                     /gene="pyrI"
                     /locus_tag="ON345_000703"
     CDS             4697..5158
                     /gene="pyrI"
                     /locus_tag="ON345_000703"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR3070812.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPISSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5231..5617
                     /gene="ridA"
                     /locus_tag="ON345_000704"
     CDS             5231..5617
                     /gene="ridA"
                     /locus_tag="ON345_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR3070813.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5823..8519)
                     /gene="mgtA"
                     /locus_tag="ON345_000705"
     CDS             complement(5823..8519)
                     /gene="mgtA"
                     /locus_tag="ON345_000705"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR3070814.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8660..8713)
                     /gene="mgtL"
                     /locus_tag="ON345_000706"
     CDS             complement(8660..8713)
                     /gene="mgtL"
                     /locus_tag="ON345_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR3070815.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8898..9845
                     /gene="treR"
                     /locus_tag="ON345_000707"
     CDS             8898..9845
                     /gene="treR"
                     /locus_tag="ON345_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR3070816.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPKLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASIGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9964..11385
                     /gene="treB"
                     /locus_tag="ON345_000708"
     CDS             9964..11385
                     /gene="treB"
                     /locus_tag="ON345_000708"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR3070817.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKTARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11435..13090
                     /gene="treC"
                     /locus_tag="ON345_000709"
     CDS             11435..13090
                     /gene="treC"
                     /locus_tag="ON345_000709"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR3070818.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESERYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWISLGDNYQEINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13484..15622
                     /gene="nrdD"
                     /locus_tag="ON345_000710"
     CDS             13484..15622
                     /gene="nrdD"
                     /locus_tag="ON345_000710"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR3070819.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDETTFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15807..16271
                     /gene="nrdG"
                     /locus_tag="ON345_000711"
     CDS             15807..16271
                     /gene="nrdG"
                     /locus_tag="ON345_000711"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR3070820.1"
                     /translation="MNFHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16272..16562)
                     /locus_tag="ON345_000712"
     CDS             complement(16272..16562)
                     /locus_tag="ON345_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3070821.1"
                     /translation="MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKL
                     RDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGKRENSAVYEDATKRLDE"
     gene            complement(16552..16794)
                     /locus_tag="ON345_000713"
     CDS             complement(16552..16794)
                     /locus_tag="ON345_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR3070822.1"
                     /translation="MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRM
                     PVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL"
     gene            complement(16986..17372)
                     /gene="cybC"
                     /locus_tag="ON345_000714"
     CDS             complement(16986..17372)
                     /gene="cybC"
                     /locus_tag="ON345_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR3070823.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17555..18907)
                     /gene="pmbA"
                     /locus_tag="ON345_000715"
     CDS             complement(17555..18907)
                     /gene="pmbA"
                     /locus_tag="ON345_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR3070824.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            19001..19552
                     /locus_tag="ON345_000716"
     CDS             19001..19552
                     /locus_tag="ON345_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR3070825.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19635..21008)
                     /gene="mpl"
                     /locus_tag="ON345_000717"
     CDS             complement(19635..21008)
                     /gene="mpl"
                     /locus_tag="ON345_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR3070826.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21184..22182
                     /gene="fbp"
                     /locus_tag="ON345_000718"
     CDS             21184..22182
                     /gene="fbp"
                     /locus_tag="ON345_000718"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR3070827.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22215..23210)
                     /gene="yjfF"
                     /locus_tag="ON345_000719"
     CDS             complement(22215..23210)
                     /gene="yjfF"
                     /locus_tag="ON345_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR3070828.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCVFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(23197..24219)
                     /locus_tag="ON345_000720"
     CDS             complement(23197..24219)
                     /locus_tag="ON345_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3070829.1"
                     /translation="MPQSLPDTTPSKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24233..25735)
                     /locus_tag="ON345_000721"
     CDS             complement(24233..25735)
                     /locus_tag="ON345_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3070830.1"
                     /translation="MNTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLQSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAQRFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELAGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25868..26824)
                     /gene="ytfQ"
                     /locus_tag="ON345_000722"
     CDS             complement(25868..26824)
                     /gene="ytfQ"
                     /locus_tag="ON345_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR3070831.1"
                     /translation="MWKRLLVVSAVSAAMSSIALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27134..27664
                     /gene="ppa"
                     /locus_tag="ON345_000723"
     CDS             27134..27664
                     /gene="ppa"
                     /locus_tag="ON345_000723"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR3070832.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27743..28093)
                     /gene="chpB"
                     /locus_tag="ON345_000724"
     CDS             complement(27743..28093)
                     /gene="chpB"
                     /locus_tag="ON345_000724"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HCR3070833.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAAGE
                     VVEEALLRLQAVVE"
     gene            complement(28087..28338)
                     /locus_tag="ON345_000725"
     CDS             complement(28087..28338)
                     /locus_tag="ON345_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ChpS family
                     antitoxin"
                     /protein_id="HCR3070834.1"
                     /translation="MRITIKKWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGDEIW"
     gene            complement(28550..28891)
                     /locus_tag="ON345_000726"
     CDS             complement(28550..28891)
                     /locus_tag="ON345_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR3070835.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28894..32673)
                     /gene="tamB"
                     /locus_tag="ON345_000727"
     CDS             complement(28894..32673)
                     /gene="tamB"
                     /locus_tag="ON345_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR3070836.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVL
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGDKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTISALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWKIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVSI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDISPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYMEAVSGVDQALDLLYQFEF"
     gene            complement(32670..34403)
                     /gene="tamA"
                     /locus_tag="ON345_000728"
     CDS             complement(32670..34403)
                     /gene="tamA"
                     /locus_tag="ON345_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR3070837.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGVVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34609..35247
                     /gene="msrA"
                     /locus_tag="ON345_000729"
     CDS             34609..35247
                     /gene="msrA"
                     /locus_tag="ON345_000729"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3070838.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35570..36913
                     /locus_tag="ON345_000730"
     CDS             35570..36913
                     /locus_tag="ON345_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3070839.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPTFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36975..37181)
                     /locus_tag="ON345_000731"
     CDS             complement(36975..37181)
                     /locus_tag="ON345_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR3070840.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37506..38060
                     /locus_tag="ON345_000732"
     CDS             37506..38060
                     /locus_tag="ON345_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR3070841.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(38150..>38921)
                     /locus_tag="ON345_000733"
     CDS             complement(38150..>38921)
                     /locus_tag="ON345_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070842.1"
                     /translation="DSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKLLISFSLK
                     DNGAEYTMTLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSD
                     IPKRIKGYIRQAFGDVSFGYALLEDIVSKLKRGKFELQIPGCGIKECDGWYIYEKIID
                     DNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVD
                     AIVSANLAADSNITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         9468 a        10414 c         9564 g         9475 t
ORIGIN      
        1 attaatagca gatgttcttt ccttattgct aataatgcat tattcaatgg tttttctaat
       61 aaatccgtaa tcgtagaatc aactgaatct ttacaactca cctgcgagtt attcaagtcc
      121 tgagaattat ttaactcctg cggaatattt aatagacgag ctatttcctt gttagcttcg
      181 cgttggtaac acgccagaat cttgttagta atcaggtgta atgaactctc atcttttctt
      241 tcaaaggact tcccttcata acgattagtc gaaccatcag gttgtgtaaa tataatgact
      301 gtatgtcctt cacgaatccc attattaaaa gctgaggcaa gtgtaatttc tatttccacc
      361 ttagataaat ccgctcgctc cccttcagga ttattcataa caaactgcat tatttcactg
      421 ccatcattat ttttgatata agatgaggac tcaatatgag ccatcatatt tctacccgct
      481 tcgttggagc gtttgctaaa atccacataa cccagggtaa atatttttac caaaaactcc
      541 aaaaatgtct taggatcaag gatatttctt gtgttatcct gcccctcggc aattcgggca
      601 aaattattca ttccaacacg ccaggtattt aaaacttcac ttttttgttt aaaattgtcg
      661 gctaatgtaa tgtgttgaga ataattcaaa tctgatattt tgctcatgac ggactccttg
      721 agttaataag aacgtctaag cattacaaat tctactcaca aaaaaatatc aaaaaacgct
      781 cttttcacaa actcgccgta aacctggagt tatttacggc gaaaagtcat cacgccatca
      841 gcatcttcac tactgctttg cgaacctggg ctggcgcgcc cactgcgcac agcggtttat
      901 gcacttcccc cggataaaac acaacaaaat caccttcatt aaggataact gttttctcat
      961 caacgccttc cggcaaaaat gcgatgtctt tatcagctaa ccagtcggta tccggcgtgc
     1021 ctgcaggttg cgtgctgaag gtcatgcctt cctgaccttt taacacaatc tgaatgtcga
     1081 gatagcgggc atgatactcc gcacggcgag cttcgtacgg ctcggtcata tcttccgaga
     1141 taagataaaa cagacgattg ccttcgatat cgtgcttacc ctttggcgtt tctgccgtaa
     1201 cgtgcgcttt gatatgctca atcgcctggc gtaactcctg gggtagccac ggctgaagat
     1261 tatgaatatt tccgatgatc atcttttcca ctccttaatt aaaacaatgt ttcatttcta
     1321 tcgttatacg aaaagatgat caattccgcc agccttaacg cgtgaattat tgtcttatca
     1381 caacttatcg gcaggcgcgt tcccgccttt cggatcaagg ccagcatcac tccttgagaa
     1441 gagtttttat cgcttccccc gccagtgcag ctgttgcaca gagttgttcg atggagtgcc
     1501 cctcccgtgc cttcgcagaa agccctgaca tcaccgtact cataaaatca gtcacacatt
     1561 gtgcgctttg tggatgccgc ctggcgatat agtcataaat ggtcgtttct gcggcgtgat
     1621 aatattgaac ggcaatatca cgcgctagtg gatcatgact atgaatacct tcaagaacca
     1681 tacagccagc gcagccgcca ttttggctat atctgcgcgc cgcttctttt aatacctcaa
     1741 ccaggcactc gcctactgga cgatcatcac gaagaatatc ggcaagcgga atagcttccg
     1801 taccgacgta ttcattgagt acacggctaa ataacccagc tttactgcca aaagccgcgt
     1861 agaggctcgg ggggttaata ccaagataat cagtaacttc agcaacactg acagcatcga
     1921 aacctttttg gtgaaaaagc acttttgccg cagagactgc ctgctcagga gcgaaacgtc
     1981 tgggacgacc tggaacgcga gattgttttt tagtgaccat tacaaaacct gttgacagaa
     2041 agttaaaaca gttttgtaat gcatgttaca taataaatca aggagtcctt atgggcgctt
     2101 ttacaggtaa gacagttctc atcctcggtg gcagtcgtgg tatcggtgcc gctatcgtac
     2161 gtcgtttcgt caccgatggg gccaatgtac gattcaccta tgcggggtcg aaagatgccg
     2221 ctaaacgcct ggcacaagag actggagcga cagcagtatt cacagatagt gctgacagag
     2281 acgctgtcat tgatgtcgtt cgtaagagcg gcgcattgga tatcctggtg gtaaatgcag
     2341 gtattggcgt ctttggcgag gccctggaat taaatgccga cgatattgat cgccttttca
     2401 aaatcaatat tcatgctcct tatcatgcct ctgttgaagc cgcccggcag atgcccgaag
     2461 gcgggcgcat cttaatcatc ggctccgtga atggcgatcg tatgcctgtt gcaggcatgg
     2521 ctgcttatgc cgccagcaaa tctgccctgc aaggcatggc gcgcgggctg gcccgtgatt
     2581 ttggaccgcg tgggatcacc attaacgtcg tccagccagg gccaattgat accgacgcta
     2641 atcccgccaa cgggccaatg cgcgatatgt tgcatagttt gatggctatc aaaagacatg
     2701 ggcaaccgga agaggtcgct ggtatggtcg catggttagc agggccagaa gccagttttg
     2761 ttaccggcgc gatgcatacc attgatggcg cgtttggcgc ataaccgact acgctcaatt
     2821 aagcccagcc attatccatg atgtctgggt tttgtttact cacgtcgtcc gctaaaagcg
     2881 gctcccggta aagagaaatc ttctgttcat gtcgtaaatc ttgaggataa ccaaatggta
     2941 gaaagaaccg ctgttttccc tgctggccga cattcactat atgctgagca tcgttattct
     3001 gcggctattc gttccggcga tttgctgttt gtttccgggc aagtaggaag tcgagaggac
     3061 ggaacaccag aacccgattt tcagcaacaa gtcagactgg catttgataa tttgcatgcg
     3121 accctggcag ctgcgggatg cacttttgac gatatcattg atgttacgag cttccatacc
     3181 gatccagaaa accaatttga agacatcatg acggtgaaaa atgaaatatt tagcgcccca
     3241 ccttatccaa actggacggc ggtgggtgtt acatggctgg caggctttga ttttgaaatt
     3301 aaagtgatag cgcgcatccc tgagcagtaa gcaatagtgt tagccgttcg ttttcacact
     3361 ccgccctata agtcggatga atggaataaa atgcatatct gattgcgtga aagtgaaaaa
     3421 gaaaaaagca ggaaatgtct gcaattattg ataccgaacg accgttcccc tgcagaatca
     3481 caacaaataa aaatgcatgt attttgactt ataattcaaa taaaccgttt gcgctgacaa
     3541 aatattgcat caaatgcttg caccgcttct gacgatgagt ataatgccgg acaatttgcc
     3601 gggaggatgt atggttcagt gtgttcgaca ttttgtctta ccgcgtctga aaaaagacgc
     3661 tggcctgccg tttttcttcc cgttgatcac ccattcccag cccctcaatc gaggggcttt
     3721 tttttgccca ggcgtcagga gataaaagat ggctaatccg ctatatcaga aacatatcat
     3781 ttccataaac gaccttagtc gcgatgacct taatctggtg ctggcgacag cggcgaaact
     3841 gaaagcaaac ccgcaaccag agctgttgaa gcacaaagtc attgccagct gtttcttcga
     3901 agcctctacc cgtacccgcc tctctttcga aacatctatg caccgcctgg gtgccagcgt
     3961 ggtgggcttc tccgacagcg ccaatacatc actgggtaaa aagggcgaaa cgctggccga
     4021 taccatttcg gttatcagca cttacgtcga tgcgatagtg atgcgtcatc cgcaggaagg
     4081 tgcggcgcgc ctggccaccg agttttccgg caatgtaccg gtactgaatg ccggtgatgg
     4141 ctccaaccaa catccgacgc aaaccttgct ggacttattc actattcagg aaacccaggg
     4201 gcgtctggac aatctccacg tcgcaatggt tggtgacctg aaatatggcc gcaccgttca
     4261 ctccctgact caggcgttag cgaagttcga cggcaaccgt ttttacttca tcgcgccgga
     4321 cgcgctggca atgccgcaat acattctgga tatgctcgat gaaaaaggga tcgcatggag
     4381 tctgcacagc tctattgaag aagtgatggc ggaagtagac atcctgtaca tgacccgcgt
     4441 gcaaaaagag cgtctggacc cgtccgagta cgccaacgtg aaagcgcagt ttgttcttcg
     4501 cgccagcgat ctccacaacg ccaaagccaa tatgaaagtg ctgcatccgc tgccgcgtgt
     4561 tgatgagatt gcgacggatg ttgataaaac gccacacgcc tggtacttcc agcaggcagg
     4621 caacgggatt ttcgctcgcc aggcgttact ggcactggtt ctgaatcgcg atctggtact
     4681 gtaaggggaa atagagatga cacacgataa taaattgcag gttgaagcta ttaaacgcgg
     4741 cacggtaatt gaccatatcc ccgcccagat cggttttaag ctgttgagtc tgttcaagct
     4801 gaccgaaacg gatcagcgca tcaccatcgg tctgaacttg ccttctggcg agatgggccg
     4861 caaagatctg atcaaaatcg aaaatacctt tttgagtgaa gatcaggtag atcaactggc
     4921 attgtatgcg ccgcaagcca cggttaaccg tatcgacaac tatgaagtgg tgggtaaatc
     4981 gcgccccagt ctgccagagc gcatcgacaa tgtgctggtc tgcccgaaca gcaactgtat
     5041 cagccatgcc gaaccgattt cttccagctt tgccgtgcga aaacgcgcca atgacatcgc
     5101 gctcaaatgc aaatactgtg aaaaagagtt ttcccataat gtggtgctgg ccaattaatt
     5161 gcggttggta ataaaagtct ggctccctat aatgagccag actttttacc gctgtaataa
     5221 aggagaaatc atgagcaaaa ctatcgcgac ggaaaatgca ccggcagcta tcggtcctta
     5281 cgtacagggc gttgatctgg gcaatatgat catcacctcc ggtcagatcc cggtaaatcc
     5341 gaaaacgggt gaagtaccgg cagacgtcgc tgcacaggca cgtcagtcgc tggataacgt
     5401 aaaagcgatc gtcgaagccg caggcctgaa agtgggcgac atcgttaaaa ccaccgtgtt
     5461 tgtgaaagat ctgaacgact tcgcaaccgt taatgctacc tacgaagcct tcttcaccga
     5521 acacaatgcc accttcccgg cacgttcttg cgttgaagtt gcccgtctgc cgaaagacgt
     5581 gaaaattgag atcgaagcga tcgctgttcg tcgctaatct tgatggaaat ccgggctatc
     5641 atgcccggat taagtctgat gacaaacgca aaatcgcctg atgcgctacg cttatcaggc
     5701 ctacgtgatt cctgcaattt attgaatttg ttggccggat aaggcattta cgccgcatcc
     5761 ggcatgaaca aagcttactt tgtctgcaat ctgaatcggg gctatcgtgc ccagtttatt
     5821 ctttactgcc agccgtaacg acggctataa aatcctttca ccaactgagt taatgtcata
     5881 taccctgcca gaatcgcaac cagccacggg aaatagctta acggcagcgc ctgcagttgc
     5941 agataactgg ccagcggtga aaacggcaat gcgatcccga caatcatcac gatcacggtc
     6001 atgatcatta acggccacga tgcacagctc tgaataaacg gcacacggcg ggtgcggatc
     6061 atatgcacaa tcagcgtttg cgacagtaag cccaccacaa accatcccga ctggaacagc
     6121 gtttgcgttt ccggcgtgtt ggcatggaat acccaccaca tcaggcaaaa cgtcagaata
     6181 tcgaagatcg agctgatcgg tccgaagaag atcataaagc gccccagatc cgccggattc
     6241 caacgctgcg gcttttgaat ttgctcgtcg tcgacgttat caaacgggat cgccacctgt
     6301 gacacatcat acagcaggtt ctgaataagc aagtgtaacg gcaacatcgg caggaagggc
     6361 aagaaagcac tcgccaccag cacgctgaac acattaccga agttggagct cgccgtcatt
     6421 ttgatgtatt tcagcatgtt ggcgaaagtg cgacgtccct caataacccc ctcttccagc
     6481 accatcaggc ttttttccag caggatgata tcagccgctt cacgggcaat atctaccgcg
     6541 ccgtccacag aaatgccgat atccgcagca cgtaaggcgg gcgcatcatt aataccatcg
     6601 cccataaagc caaccacatg cccttcgcgc ttcagtaagg tcacaatgcg ttctttatgc
     6661 atcggcgtca ggcgagcaaa cagcgtggtg cgctgtgcga gatttgccag ttcatcgtca
     6721 gatagcgttt caatatcact accaatgacc acctctcccg cgtccagccc cacttcatgg
     6781 cacactttcg ctgcaactaa ctcactgtcg ccagtaagga tttttacggt aatgccactc
     6841 gcttttaatg ccttcagtgc cggagcagtt gtctcttttg gcggatcaag gaaagcaata
     6901 tatccttcga ggatcaggtc ggattcatcc gctcgctggt aatcgccttc acgcgctggc
     6961 aggtatttcg tcgccaccgc aaccacgcgc agcccctgac gattcagcgt atcggtaacc
     7021 cgcttaatct tacgcagcat ggtgtcatcg agtggtacaa tctcgccatt gtgacgcacc
     7081 tgcgaacaca cattgaggat ttcctgcaat gcgcctttgc aaaccagctg atggtgttcg
     7141 gtattttctg ccactaccac cgacatccgg cgacgctcga aatcaaacgg aatctcatca
     7201 attttttgcc aacggctggc cagcgagcgc gctgactctt catccgtacc ttcgagcacc
     7261 gcggtatcaa gcaggttttt aagcccggtc tggtaatgac tgttcaacca cgcgctatgc
     7321 agcacgcgtt cgctggtttt accggagata tcggtatgat tctccagcac aattttgtcc
     7381 tgcgtcaggg tgccggtttt atcagtgcac agaatatcca ttgcgccaaa gttctgaatg
     7441 gcatccagat gcttgacgat cactttctgt ttcgacagct ttactgcgcc gcgcgccagc
     7501 gtcgaggtaa caatcatcgg caacatttct ggcgttaagc ctaccgcgac cgaaagcgca
     7561 aacagtgccg cttcccacca gtcacctttg gtgtaaccgt tgataagcaa caccaccgga
     7621 gccatcacca gcataaagcg aatcagcagc atactgacgc ggctgatccc ttgctgaaag
     7681 gcattcggct cgctttcctg ctcactaaca cgccccgcca gttgaccaaa ccaggtgttg
     7741 gctcctgtag caatcaccat tgcttgtgcc gtgccgctca ccacggtggt gcccataaaa
     7801 cacagggtgt cgcactccag cggattgctg tgctccggct ggcgagtagt agcggctttt
     7861 tccacgggca gagactcacc ggttaacgac gcctgagcga cgaacagatc ccgcgcctgc
     7921 aagatacgta aatctgccgg gatcatatcc cctgccgcca gttttataat atcgccgggc
     7981 accagctggt cgatcgggat ctccagccag ccattttcgc ctttgtcgtt aattacgcgc
     8041 agcaccgtcg cagtattgct gaccatcgct ttcagggcat ctgccgcttt agtggaacgt
     8101 gcttcctgaa taaagttcag caacgtagaa atagcgacca ttagcgcgat aacgcccgcg
     8161 gcaaataaat cttccgtggc gtaagaaata gcgccgagaa tggtgagtaa aatattaaag
     8221 gggttgcgat agcagaccca taaatgaacc caccacggcg acggttgttg tgcgggtaat
     8281 ttattttcac catgttgttc gcgggcagat tccacttccg cctgatttaa tccttccgga
     8341 tgggtgtcga acgttttcca caattcttct tcgggcatca ccgccatttt caggcaatgc
     8401 gcacttaagg acggcgggac cgccgtattc actgtctgct gcgcgccagg caatggatcg
     8461 cgatgaacca gacgggaagg taaatggcga atgaaccggg taaaaatttc tttaaacata
     8521 aggagtccct ccgcactgtc tgaaacagta cagaaatgtc ttcaggcgtg gcgaagcctt
     8581 acctgaaggt aataaagctg agcagggacg ttaaaacgtc acggccttac gcatccggta
     8641 acgccgtgaa aagacgggct taccggaaaa gttttaatct ccgtcgaggg agaggcgtgg
     8701 gatcaggttc catataacct ccggtaagtg aaaaaagcgc cgcaaataat aaggagaatc
     8761 caataatatt cgtagcatct atggcgatat aatacctgca ggcaactaaa ccaacgataa
     8821 accagacttt acccattgct gaatgcacgg gtaaagttag gctcaaataa ttaaacaaca
     8881 cgttacggga caacaggatg caaaatcggc tgaccatcaa agacatcgcg cgcttaagcg
     8941 gcgtggggaa atctacggtt tcccgggtgc tgaataacga aagcggcgtg agccagcgca
     9001 cgcgcgagcg tgttgaagca gtgatgaatc agcatggatt ttccccttcc cgctctgcgc
     9061 gcgctatgcg tgggcaaagc gataaagtgg tcgccatcat tgttacccgt ctggattcgt
     9121 tgtcagaaaa tctcgccgtt caaaccatgc tgccagcgtt ctatgaacaa ggttacgatc
     9181 caatcatgat ggaaagtcag ttttccccaa aattagttgc cgaacatttg ggggtgctga
     9241 aacggcgtaa tatcgacggc gtagtgctgt tcggttttac cggcataacc gaagaaatgt
     9301 tagcccactg gcagtcatcg ctggttctgc tggcgcgtga cgcaaaaggc tttgcttcgg
     9361 tctgttatga cgacgaaggg gcaatcaaga tcctgatgca acggctgtat gaccaggggc
     9421 atcgtaatat cagttatctc ggcgtgccgc atagtgacgt gacgaccggt aagcgacgtc
     9481 acgaagccta cctggcgttc tgcaaagcgc ataaactgca tcccgttgcc gccctgccag
     9541 gccttgctat gaagcaaggc tatgagaacg tagcaaaagt gattacgcct gaaactaccg
     9601 ccttactgtg cgcaaccgac acgctggcac ttggtgcaag taaatacctg caagagcaac
     9661 gcatcgacac cttgcaacta gcgagcatcg gtaatacgcc gttaatgaaa ttcctccatc
     9721 cggagatcgt caccgtagat cccggttacg ccgaagctgg acgccaggcg gcctgccagt
     9781 tgatcgcaca ggtaaccggg cgcagcgaac cgcaacaaat catcatcccc gccaccctgt
     9841 cctgatcgtt tcctgaacga taaattgtga tcttcgctgc gtttcgggaa cgttcccgtt
     9901 tttaaatttt tccgcgcaat atattctgca gccaaccaaa aatgtcatct gccatggggc
     9961 tttatgatga gcaaaataaa ccaaacggat atcgatcggt tgattgaact ggtcggcggg
    10021 cgcggcaata ttgcgacggt gagccactgt attactcgtc tgcgctttgt cctcaaccaa
    10081 ccggccaatg ccagaccgaa agaaattgag caactcccca tggtgaaagg ctgtttcacc
    10141 aatgccgggc aatttcaggt ggtaattggc accaacgtgg gtgattacta tcaagcactg
    10201 atagcgtcaa ccggacaggc gcaggttgat aaagagcagg taaaaaaaac cgcacggcag
    10261 aatatgaaat ggcatgagca gttgatctct catttcgcgg agatcttctt cccgttgctg
    10321 cccgcgttga ttagcggcgg tttgatcctc ggttttcgca atgtgatcgg cgatttgccc
    10381 atgagcaacg gtcaaacgct ggcgcaaatg tacccttccc tgcaaacgat ctacgatttt
    10441 ctgtggttga tcggtgaagc gatcttcttc tacctgccgg tcgggatttg ctggtcagcg
    10501 gtgaaaaaaa tgggcggcac gccgatcctt ggtatcgtgc ttggcgtgac actggtttcc
    10561 ccccagctga tgaacgctta tctgctcggg cagcagctgc cggaagtgtg ggactttggc
    10621 atgttcagca tcgccaaagt gggctatcag gcgcaggtga tcccggcact gttagccggg
    10681 ctggcgctgg gcgttattga aactcgcctt aaacgcatcg taccggatta cctctatctg
    10741 gtggtggtgc ccgtctgttc gctgatcctc gcggtgttcc tcgcccatgc gctgattggt
    10801 ccgtttggtc gcatgattgg cgatggcgtt gcctttgcgg tacgtcacct gatgaccggc
    10861 agctttgctc cgattggtgc ggcattgttt ggcttcctgt acgcgccgct ggtgatcacc
    10921 ggcgtacacc agaccaccct tgctattgat ttgcagatga ttcaaagcat gggtggcacg
    10981 ccagtgtggc cgctgattgc gctgtcgaat atcgctcagg gctccgccgt gataggcatt
    11041 atcatttcca gccgcaagca caatgaacgc gagatctccg tgcctgccgc tatctccgcc
    11101 tggcttgggg tcactgagcc tgcaatgtac ggcatcaacc tgaaatatcg cttcccgatg
    11161 ctgtgcgcga tgattggttc tggtctggca ggattactat gcggcctgaa cggcgttatg
    11221 gcgaatggta tcggcgtagg cggcctgccg ggaattctct cgattcaacc gagctactgg
    11281 caggtatttg cgctggcaat ggttatcgcc atcatcatcc cgattgtact cacctcgttt
    11341 atctatcagc ggaaataccg cctgggcacg ctggatattg tttaattttc ttcggggcgc
    11401 aattgcgccc cctcgcattt gcaggaataa cgtaatgact aatcttcccc actggtggca
    11461 aaacggcgtt atctaccaga tttatccaaa gagttttcag gacaccacgg gtagcggcac
    11521 cggcgattta cgtggcgtta tccaacgcct ggactatctg cataaactgg gcgttgatgc
    11581 catctggcta accccctttt atatctctcc ccaggtcgat aacggttacg acgtagcgaa
    11641 ctatacggcg attgatccca tctacggcac gctggacgat tttgacgaac tggtgacgca
    11701 ggcaaaatcg cgcgggattc gtatcattct cgatatggtg tttaaccata cctctaccca
    11761 acatgcctgg tttcgcgagg cgctgaacaa agaaagccct taccgccagt tttatatctg
    11821 gcgcgatggc gaaccagaaa cgccaccgaa caactggcgt tcaaaatttg gcggtagtgc
    11881 gtggcgctgg catgcggaaa gcgaacggta ctatttgcat ctctttgcac cagaacaggc
    11941 ggatctcaac tgggagaatc cagcggtacg cgcagagctg aaaaaagtct gtgaattctg
    12001 ggccgatcgt ggggtcgacg ggttgcgcct ggatgtggtg aatctgatct ccaaagaccc
    12061 gcgtttccct gaagacctgg atggcgacgg gcgtcgcttc tacaccgacg ggccacgagc
    12121 acacgagttt ttgcacgaga tgaaccgcga tgtgtttacg ccacgcgggt taatgaccgt
    12181 aggtgaaatg tcctccacca gccttgagca ttgccagcga tacgcggcac tgacaggcag
    12241 tgaattgtcg atgaccttta attttcatca cctgaaggtc gattatcccg gtggtgaaaa
    12301 atggacgctg gctaaacctg actttgtggc gttgaaaaca ttgttccgcc actggcaaca
    12361 aggaatgcac aacgtggcat ggaatgcctt gttctggtgt aaccacgatc agccgcgcat
    12421 tgtttctcgc tttggtgatg aaggtgaata ccgcgtgcct gcggcaaaaa tgctggcgat
    12481 ggtgctgcat ggcatgcagg gaacgccgta tatctaccag ggcgaagaga ttggcatgac
    12541 caacccgcat ttcacgcgca ttactgacta tcgcgacgtg gagagcctca atatgtttgc
    12601 cgagctgcgc aacgatggtc gtgatgccga cgagttattg gcaatcctcg ccagtaaatc
    12661 ccgtgacaac agccgcacgc ccatgcaatg gagcaacggc gataatgccg ggtttacggc
    12721 tggcgaacca tggattagcc tgggcgataa ctatcaggaa atcaacgtag aagctgcgct
    12781 gaccgatgag tcctcggtgt tttacaccta ccaaaagtta atcgcactgc gtaagcaaga
    12841 agccgtcctg acatggggcg attaccagga tctgctgcca aacagccctg tattgtggtg
    12901 ctatcgccgt gagtggaagg ggcaaacctt gctggtcatt gccaacctta gccgtgagat
    12961 ccaaccctgg caaccagggc aaatgcgcgg caactggcag cttgtgatgc ataactacga
    13021 agaagcctca ccacaaccct gcgccatgac tttacggcct tttgaggctg tctggtggtt
    13081 acaaaagtaa atcttcgcta agccccggta atgccgctca ttccggggtt ttgtctgtca
    13141 gcatgttgtt tttgttgatt tttcaaccag caaattcatt aaaaaattta catatcgctg
    13201 tagcgcccgt catccgtacg ctctgctttt tactttgagc tacatcaaaa aaagttcaaa
    13261 catccttgat gcaaagcact atatatagac tttaaaatgc gtcccaaccc aatatgttgt
    13321 attaatcgac tataattgct actacagctc cccacgaaaa aggtgcggcg ttgtggataa
    13381 gcggatggcg attgcggaaa gcaccggaaa acgaaacgaa aaaaccggaa aacgcctttc
    13441 ccaatttctg tggataacct gttcttaaaa atatggagcg atcatgacac cgcatgtgat
    13501 gaaacgagac ggctgcaaag tgccgtttaa atcagagcgc atcaaagaag cgattctgcg
    13561 tgcagctaaa gcagcggaag tcgatgatgc cgattattgc gccactgttg ctgcggttgt
    13621 cagcgagcag atgcagggcc gcaaccaggt ggatatcaac gagatccaga ccgcagttga
    13681 aaaccagctg atgtcgggtc catacaaaca actggctcgt gcttacattg agtaccgtca
    13741 cgatcgcgac attgaacgtg aaaaacgcgg tcgcctgaac caggagatcc gtggtctggt
    13801 cgagcagacc aactcctcgt tactcaacga aaacgccaac aaagacagca aggtgattcc
    13861 aacccagcgc gatctgctgg ccgggatcgt ggctaaacac tatgcacgcc agcacctgct
    13921 gccgcgtgac gtggtgcagg cacatgaacg tggtgatatt cactatcacg atctcgatta
    13981 ctcgccgttc ttcccgatgt tcaactgcat gttgatcgac ctgaaaggca tgctgaccca
    14041 gggctttaaa atggggaacg ccgagattga accgccgaag tcgatctcta cggcaaccgc
    14101 tgtaactgcg cagattattg ctcaggtcgc cagccatatt tatggcggca ccaccattaa
    14161 ccgtatcgat gaagtgctgg caccgtttgt caccgccagc tacaacaaac atcgcaagac
    14221 cgcagaagag tggaacatcc cggacgccga aggctacgct aactctcgta ccatcaaaga
    14281 gtgctacgat gccttccagt cgctggagta cgaagtaaac acactgcaca ccgccaacgg
    14341 tcaaacaccg tttgtgacct ttggttttgg tctgggcacc agctgggagt cgcgcctgat
    14401 tcaggaatct atcctgcgta atcgtatcgc aggcctcggt aaaaaccgta aaactgcggt
    14461 gttcccgaaa ctggtgtttg cgattcgcga tggcctgaac cataaaaaag gtgatccgaa
    14521 ctacgacatc aaacagctgg cgctggagtg cgcaagcaag cgcatgtatc cggatatcct
    14581 gaactacgat caggtagtga aagtcaccgg ttcgtttaaa accccgatgg gctgccgcag
    14641 cttcctcggc gtgtgggaaa atgaaaacgg cgagcagatc cacgatggtc gtaacaatct
    14701 cggcgtgatc agcctgaacc tgccgcgtat tgctctggaa gcaaaaggcg atgaaaccac
    14761 cttctggaag ctgctggatg aacgtctggt gctggcacgt aaggcgctga tgacccgtat
    14821 cgcccgtctg gaaggcgtga aggcacgcgt agctcctatc ctctatatgg aaggtgcttg
    14881 tggcgtgcgt ctgaatgctg acgatgatgt ttctgaaatc ttcaaaaacg gtcgtgcatc
    14941 tatttcgctg ggttacatcg gcatccacga aaccattaac gcgctgttcg gcggcgagca
    15001 tgtttatgac aacgagcagc ttcgcgcgaa aggtatcgcg attgttgaac gtctgcgtca
    15061 ggcagtggat cagtggaaag aagaaacggg ttatggtttc agtctctaca gcacgccgag
    15121 tgaaaacctg tgcgaccgct tctgccgtct cgataccgct gagtttggcg tggtgccggg
    15181 cgtgaccgat aaaggttact acaccaacag tttccacctt gatgtggaga aaaaggtgaa
    15241 cccgtatgac aagatcgact ttgaagcgcc ttacccgccg ctggcgaacg gtggtttcat
    15301 ttgctacggc gagtatccaa acattcagca caacctgaag gcgctggaag atgtctggga
    15361 ttacagctat cagcatgtac cgtattacgg caccaatacg ccgatcgatg agtgctacga
    15421 gtgtggcttt actggcgagt tcgagtgcac cagcaaaggc ttcacttgcc cgaaatgtgg
    15481 taaccatgac gcctcccgtg tgtcggtaac tcgccgcgtg tgcggatatt taggtagccc
    15541 ggatgcacgt ccatttaacg ccggtaagca ggaagaagtt aagcgccgcg ttaaacattt
    15601 ggggaatggg cagataggtt aatccttccc cctcacccta accctctccc tcaagggaga
    15661 gggtaccgtt ccgtgcccct tcctgattcc cgacataatg aattgcctct ggccagttgg
    15721 tgtcgtcgca aggtggcaaa tgcgtagcat cgggggactt cgcccgtacg caccagagca
    15781 gtcatggcgt caattgagaa tcgaaaatga actttcatca gtactatcct gtcgacatcg
    15841 tcaacggccc cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg
    15901 gttgctataa caaaagcaca tggcgggtaa attccggtca gccatttacc aaagcaatgg
    15961 aagaccagat cattaacgat ctaaatgaca ctcgcatcaa acgccagggg atttccctct
    16021 ccggcggcga tccgctgcat ccgcaaaacg tgccggatat tctgaaactg gtacaacgaa
    16081 tccgcgccga atgtccgggt aaagacatct gggtgtggac aggctataaa ctcgacgaac
    16141 tcaacgctgc gcaaatgcag gttgttgatt tgattaacgt actggtcgac ggcaaatttg
    16201 tgcaggattt aaaagaccct tccctgatct ggcgcggcag cagcaatcag gtggtgcatc
    16261 atttgcgtta gtcactcatc cagccgttta gtcgcgtctt cataaacggc tgagttctct
    16321 ctcttaccaa tagctatcac caacacgagc aactctttat cgttaacctg ataaaccagg
    16381 cgatatccct gtgtgcgcag cttaattttg tagcagtccg gtaaatcccg cagtttagcc
    16441 gatggattgc gcggcctttt cagcacatcc tcaagctttt ttttaaattg ttcccggact
    16501 gtcacgccca gtttttgcca ctctttcagc gcccgcggat caaacgccag ctcatagttc
    16561 atccagtcgg accttaatgg cctcttgtgg gttggccagt cgttccctga cagtgcgtaa
    16621 caaggcttct tcactatcac tgacagtgag cgtttttacc ggcattcgtc cattttccgc
    16681 aacgtactga aacagcagtc gcactgcttc agtaggcgtg atatttaatt tttccagcac
    16741 cgcataagcc tcatttttca gtttgtcatc caagcggacg ttaagtgtgg ccattatgcc
    16801 tccggtaatt atgaaaagtg taatgcatta tagcattaca tatgcattac attcatttct
    16861 ggaaaacgcc tcgcaaaaat gaggacgaca cgcaggaata tgccgtggtg aagatagata
    16921 ttgcggtgat tttacgaagg gagaagaaag cgttgggcgg taacgtttca ccgcccaata
    16981 aggaattaac gatacttctg gtgataggcg ttgcgggtcg ttttcagttg ctctgcggca
    17041 gcctgcgctt cttttacttt accttcattt gccagcttca gcgcgtcgtc aatctgaccg
    17101 accagaatat cgaaaccgtg gcggaagtct ttcatttccg ggctgtccgg tgatttgcct
    17161 tcgagcttcg gcggcgtcgc tttttgcgca tccagcgctg cggcgcgcat cttcgttaac
    17221 gcgtctttga cttgcgccgc gttatcggct ttttcgacca cttttaaatt gtcgttgagg
    17281 gtttccatat tgtcttcaag atcagcggca aacgacgccg aactgaatac caacgaggag
    17341 actgcaagaa tagctaacag gcttttacgc attgctcact tcctttttta tttacacttc
    17401 atccttcaca ctgcctcatc gacggctacg ctcattcgcc cgagtcacgt actgcgagta
    17461 cgctccccgg ccctcattca ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt
    17521 tttagtttat agcgcggcag gtcgcgccag ttttttactg cccggcgatt ttcatctccg
    17581 gcaacagcac agaaccacac tgtatattac tgcgtgtttc aatatcgtta ccgacggtga
    17641 caatattgcg ccacatatct tttaaattac ctgcgatggt gatttcacta accggatact
    17701 gaatttcgcc attctctacc cagaagcccg ctgcaccacg ggaataatca ccggtaatgg
    17761 cactcacgcc ctggcccatc agctccgtca ccaccagccc ggtgcccatc tctttaagca
    17821 tttgttcgaa gcttagccct tgtccggcaa tccgccagtt gtgaataccg cccgcatgtc
    17881 cggtgctttt cagccccagt ttccgcgccg aatagctggt caacagccac tgagtcagga
    17941 tgccatcttt aataatatcg cgacgctctg tgcgcacacc ttcgctgtcg aatggcgtag
    18001 aggccagccc tttcagcaga tgcggatgct cttcaatggt cagccagtcc ggcagaattt
    18061 gtttacccag cgaatccagc aggaaggtgg atttacggta aaccgatccg cccgctatcg
    18121 ctccaaccag atggccaaaa agcccggttg ccacttcatt ggcaaaaatg accggcgctt
    18181 tcatggtgga gagtttacgc ggtgacagac gcgataaagt gcgacgagca cagtcggccc
    18241 caacccactc tggcgtttgc agatcgctca tcgcacggcc aatggtgtag gcgtaatcac
    18301 gctccatatc gccattttct tcggcaatta cacagctgga gagcgaatga cgcgtagagc
    18361 agtaaccctg caacatgccg tggctgttgc cgaaaacttt gacaccgtag tggctgttaa
    18421 agctaccgcc ttcggtattg gtgatgcgtt tgtccgcctg caatgccgcc tgttctgcgc
    18481 gggccgccag ttcaatggct tcatccgggg aaacttccgc agggtggaac aagtcgagat
    18541 ccggtgcgtc aaaggccagc agctctttgt ctgccacgcc ggcacaggga tctggcgagg
    18601 tataacgggc aatatccagt gccgcctgta cggtgcgggc aatggcctgc gggcttaaat
    18661 cggtagacga tgcgctacct ttgcggttct ggtgataaac agtgatcccc aacgcgccat
    18721 cgctattgaa ttcaacattc tccacttcac cataacgcgt gcttacgcta atgccggtgg
    18781 tcttgctgac ggcaacttct gcaccgtccg atttgcctga ggccaactcc agcgcagtcg
    18841 aaactgcttc ttccagaatc ttgcgctgcg cttcaacttg agagattact ttcattgcaa
    18901 gtgccataat gtagagagtt tctctgaagt ctaacagaga accgtttttc agtgcgcatc
    18961 ttaactggta acattagcct ctttttttaa ggagcctgag atgactaagc agcccgaaga
    19021 ctggctcgac gacgttcccg gtgatgacat cgaagacgaa gacgatgaaa ttatctgggt
    19081 cagtaaaagt gaaattaaac gcgatgccga ggagctaaaa cgccttggcg cggaaattgt
    19141 tgatctgggg aaaaacgcgc tggataagat cccgttagat gcggatctgc gcgcggctat
    19201 tgagctcgcc cagcgtatta agatggaagg tcgccgccgc cagttgcagc tcatcggtaa
    19261 gatgctgcgc cagcgcgacg tagagcctat tcgtcaggcg ctggataagc tgaaaaaccg
    19321 tcacaaccag caggtggtgc tgttccataa actggaaaac ctgcgcgatc gtctgatcga
    19381 ccagggtgat gatgccatcg ctgaagtatt gaatctgtgg cccgatgccg atcgtcagca
    19441 actgcgtact ctgatccgca acgcgaagaa agagaaagaa gggaataagc cgccgaaatc
    19501 cgcacgtcag attttccagt atctgcgcga gttagcggaa aacgaagggt aattttcgtt
    19561 gttgatgtga gtatggtttg ccggatgcgg cgtaaacgtc ttatccggca atgctatatc
    19621 aggctgaggc cgaattactg cgcggcttcc gctttcttcg ccagaccatc cagcagtttc
    19681 tgatggatgc caccaaaacc gccgttgctc atcaccagaa tatggtcgcc aggctgagcg
    19741 gttttcacca ccatatctgc cagcgtatcc acatcgccac tccagtgtgc aggctgaacg
    19801 caggcttctg ccacttctgc cacctgccat ggaatatgcg ccggttgcag caggaagact
    19861 tcatcggcac gacctaatga aggtgccaga tcgtctttgc agatccccat tttcatggta
    19921 ttcgagcgcg gttccagcac agcaataatg cgcgccgtac caccaacttt gccacgcagc
    19981 gccgccagcg ttgccagaat cgccgtcggg tgatgggcaa aatcgtcata taccgtgacg
    20041 ccattcgctt caccacgcaa ctccagacgg cgacgagcat taataaacga acccagcgcg
    20101 ttagcggcat ctgccggcgc tacaccaaca tggcgagccg ccgcaatcgc catcaggcca
    20161 ttgtgcatat tatgttcgcc taccagcgac catttcactt cgcccacttt ttcgccatcc
    20221 agcaagactt cccattcgga agcatcggtg gtcagctttt tcgcctgcca gtgaccctgc
    20281 tcgcccacca gctcctgctc gctccagcag cccatcgcca tggtctgttt caggttgatg
    20341 tcgttttccg gccagataat acggccctgc cccggaacga tacgcaccag atggtggaac
    20401 tgtttctgga tcgctttcag gtcgtcaaag atatcggcgt gatcgaactc aaggttgttg
    20461 aggatcagcg tacgcgggca gtaatggaca aatttagagc gtttgtcgaa gaaggcgcag
    20521 tcatactcat ccgcttcgat aacaaagaag tcgctttcgc ccagacgcgc cgagacctca
    20581 aagttccccg gcacaccgcc gatcacaaat ccgggtttgt aaccgcactg ttccagaatc
    20641 caagtcgcca ttcccgctgt agtcgtttta ccgtgtgtac cggcgacggc cagcacccaa
    20701 cggtcacgca gtacaaaatc gtgcagccac tgcggacctg acatataagg gatgtttttt
    20761 tccagtaccg cttccacaca cggatttcca cgggtcatgg cgttgccaat aatcaccaga
    20821 tccggctgcg gttcgagctg gctggcatcg taaccctgaa tcagttcaat gccttgcttc
    20881 tcaagtaagg tgctcatcgg cggatacaca ttggcgtccg aacccgttac ttcatggcct
    20941 aactggcgcg ccagcatcgc cagaccgccc ataaacgtgc cacaaattcc taaaatatga
    21001 atgcgcatac gtcactatcc ttttttaatc tgccgctcat tttacgcata tgtcgggcaa
    21061 gtgagaaacg catttcagga taatccgcaa tttgctggcg cgattcacct gagcgcaaca
    21121 ttgagattat ttgttaagat tgttgcggtc gctttactcc ataaacattg cagggaaagt
    21181 tttatgaaaa cgttaggtga atttattgtc gaaaagcagc acgagttttc tcatgctacc
    21241 ggtgagctca ctgctttgct gtcggcaata aaactgggcg ccaagattat ccatcgcgat
    21301 atcaacaaag caggactggt tgatatcctg ggtgccagcg gtgctgagaa cgtgcagggc
    21361 gaggttcagc agaaactcga cttgttcgct aatgaaaaac tgaaagccgc actgaaagca
    21421 cgcgatatcg ttgcgggcat tgcctctgaa gaagaagatg agattgtcgt ctttgaaggc
    21481 tgtgaacacg caaaatacgt ggtgctgatg gaccccctgg atggctcgtc caacatcgat
    21541 gttaacgtct ctgtcggtac cattttctcc atctaccgcc gcgttacgcc tgtcggcacg
    21601 ccggtaacgg aagaagattt cctccagcct ggtaacaaac aggttgcggc aggttacgtg
    21661 gtatacggct cctctaccat gctggtttac accaccggat gcggtgttca cgcctttact
    21721 tacgatcctt cgctcggcgt tttctgcctg tgccaggaac ggatgcgctt cccggagaaa
    21781 ggcaaaacct actccatcaa cgaaggaaac tacattaagt ttccgaacgg ggtgaagaag
    21841 tacattaaat tctgccagga agaagataag tccaccaacc gcccttatac ctcacgttat
    21901 atcggttcac tggtcgcgga tttccaccgt aacctgctga aaggcggtat ttatctctac
    21961 ccaagcaccg ccagccaccc ggacggtaaa ctgcgtttac tgtatgagtg caacccgatg
    22021 gcattcctgg cggaacaagc gggcggtaaa gcgagcgatg gcaaagagcg tattctggat
    22081 atcatcccgg aaaccctgca tcagcgccgt tcattctttg tcggcaacga ccatatggtt
    22141 gaagatgtcg aacgctttat ccgtgagttc ccggacgcgt aatctcctgc gcctctccac
    22201 acggagaggc gtttttattg ctgcgcaatg ttgactcttg tcacaggcga actctgacga
    22261 ttctcccaca gcaccgttaa tccacgctgt aatgctataa aaataaacaa caaaataccg
    22321 atggcgattt tcgtccacca tgagctcagc gtgccatcaa agtttatgta agtctgaatc
    22381 agtccctgaa tcgccacgcc aaaaagcgtc cctaataccg ttccaacgcc accgctcaaa
    22441 agcgtaccgc caatcaccac tgaggcgata gcgtccagtt ccacacctac gcccgccagc
    22501 gcatatccgg cctgggtata aatcgagaag acaatccccg ccagcgttgc cagtccggta
    22561 gagagcatat aaatgcgaat agtggtgctg cgagtggaaa tccccatcag gttcgccgac
    22621 gttgcgttgc cgccaatggc gtacacctga ttaccaaaac gcgtacgatg cgcgaggaat
    22681 atgccgataa ccaccaccgc cagcatcagc agccccatcg cacttaagcg accgccgcca
    22741 gggattttcc acgcaaggct tgagagcgtg tcataaacag gatggtttat cgggatcgac
    22801 tcttccgaaa cgagatagct gacgccacgc aaaaagaaca ttcccgccag ggtaatgata
    22861 aatgccggga tcttcagggc gtcgatcaaa agccccataa atgcgccgaa gacacagccc
    22921 atcaccagca ccagcggaaa tgccagcagc ggcgaaaggc cgaaatcgcc aatcactttt
    22981 gccagaaaca cgccggtaaa ggcgatcacc gaaccaacgg agagatcgat cccaccggag
    23041 aggatcacaa aggtcatgcc aacagcgatg atcccaagga aagcattatc ggtcaggata
    23101 ttgcagatca ctcttgtaga agcgaaaccg ggaaactgcg tcaggcagta gagatagccc
    23161 agcacaaaga cgccgatggt gatcataagc ggcaagttac gttttatcac ggctacgcac
    23221 tcctttaatc agactgataa agcgttgcga ctggacaatc agcacgcaaa gcaccaccac
    23281 cgcttttacg acctggttca tctccggcgg aaagccagaa agcaaaattc cggtgttcat
    23341 tccctgaata atcagcgccc ccaccaccga aagcagcagg ttaaagcgcc cgcccatcag
    23401 cgatccgccg ccaatcacca ccgcgagaat ggcgtccagc tccagccata acccggcgtt
    23461 attggcatcg gcaccgcgaa tatccgccgc cacgataatg cccgcaatcg ccgcacatag
    23521 cccgctcaac acgtaggtga gcatgacgat gattcgcgtg tttaccccgg cattttttgc
    23581 cgcccgaata ttgataccaa cggcttcgat aaacatcccc agtgccgttt tgcgggtcaa
    23641 cagccagaac aggagaagcg tcagcaccgc aataatgact ggcgttggca ggaacaacag
    23701 cgatccactg ccgaaccagg agagatccgg tgagttaaat gtgacgatct gtccggaggt
    23761 gatcagttgc gccacgccgc gcccggcgac catcaggatc agggtggcga caaacggctg
    23821 aattttgagg atcgctacca gtatgccgtt ccacaatcct gccaagatgc cagtgcccag
    23881 ggcgcttaac aaaacaattg gcaggctgaa tcccgcgacc gtcatcgcag ccgttgtggc
    23941 tccggcgata gccatcaccg cccccacgga gagatcaatc ccaccagtgg cgatcaccag
    24001 cgtcatacca atcgccagta acgcaacggg agctgcacgg ttaaggatgt cgatagggct
    24061 accgaacaaa cgcccatcct gaagcactac ctgccagaaa tgcggggcca ccaggctatc
    24121 gaccagcaac accagcaata gcgccgccag ctgcggcatt cccgttggcc agcgaaagcg
    24181 ccttttcgac ggcgtagtgt ccgggagaga ttgaggcatc acactgttct ccttacgccg
    24241 caatggcgtt catgatcgcc ggaacggaaa gctctgccag cgggatctcc gccacctgtt
    24301 tgcggtcgcg catgataatc acccggtcgg catagcccgc cagctcttcc agttcggagg
    24361 agatcaccag cagcgccaga ccatcggcgc acagcgtttc aatcaggcgg atgatctcgg
    24421 catgcgcacc aacgtcaatg ccgcgggttg gctcatcgag gatcagaaat tgcggtcggg
    24481 tcagtagcca acgtgaaagc aacacttttt gctgattgcc gccggagaga aattcaatcg
    24541 gttgttcagt tgaaggtgtg cgaatgccaa gctggcggat aaagcgttgg gcaatctctt
    24601 gctgttcttt gcgggaaatc ggtcgtagcc agccgcgctg ggcctggaga gcaaggatga
    24661 tattttcccg caccgaggcg gcggcgatga tgccatcggt tttcctgtct tccgggcaga
    24721 agccgatacc cagtaccgaa gcctgatgtg gcgattgcag ggtttgcggt ttgcctttga
    24781 tcaacgccgt gccgctgtca gcaggtttga tcccgaagat cacttcggcg gtttcggtac
    24841 gtcctgatcc cagcaagcct gccaggccaa cgatctcgcc ggggcgtact tcgagatcaa
    24901 acggtgcgat cgttcctttt ttgccgtaat ttttgaacgc ggcaacgggt ttgtcgctca
    24961 gcaatgttcg tccggcacgc tgtagcgcgt gggtgtccag ctcgcgcccc agcatcattt
    25021 ttaccagttc gatctgcggt agctcgcggg tttcccgaca gcctacgaaa ctgccgttgc
    25081 gtaagacggt gatccgatcg ctgacctgat agacctgatc gagaaagtga gtgacaaaga
    25141 tcaggctgac gccgcgatcg cgcaactgac gcatcaggcc aaacagtaac tctacttcct
    25201 gagtgtcgag actggcagtg ggttcatcga ggatcagcac tttggcagag agatcgatag
    25261 cccggcaaat ggcgacgatt tgctgcatcg cgactgaaaa gcggttgagc ggttcgcgca
    25321 cgtcgaggga gaaaccgtaa gatgccatca gttcggtggc gcgcttttcc atctctttac
    25381 ggcgtagaag gccgaaacgt ttgggttcgc gacctatgaa tagattatca gcgaccgaca
    25441 tattgggtag caggttgact tcctgataga cggtgccgat gccgagttgt tgtgcatggg
    25501 cggtattttt cggtgagata gtctggcctt ccagccagat ggtgccacga tcggcgtggt
    25561 agacaccggt taatgctttg attagcgttg attttcccgc cccgttttca ccgagcagcg
    25621 ccatgatttc accacggcgc aggctgaaat caacgttgtc taacgctttg acgccgggga
    25681 aaaatttact taatccttcg gtgcggagga tttcctggtg ttggttagta ttcatagttt
    25741 cccccgttat aaagcgtgcg ccagataaga cacaacagcg tcgcatcagg catctcgcac
    25801 aaccgccgga tgcggcgtga atgccttatc cggcggccca caagtcggca tcaggcattg
    25861 atgcccctca ataccccatg tttttcttct tctctaactc ttcttttgcg gtatcaggca
    25921 gataaagggt ggatttggtc agcgtcagct tttcaggcat ggtgccgtct tttttgtatt
    25981 tctccagcgc gtcgaaagca ggacctgcca tgttcggcgt cagttcaaca ctggcgttcg
    26041 cttcgccatc aatcatcgct ttgtagatat ccggtacgcc atcaatggaa cccgtgagga
    26101 tatctttacc cggcttcagg cccgcttctt taattgcctg aattgcgcca atcaccatgt
    26161 catcgttatg ggcgtaaacc atgcagatgt ttttgccgtt gttttctgct ttgataaagc
    26221 tctccatgac ttctttgcct ttactgcggg tgaagtcacc tgactgcgag cggataattt
    26281 tgatgtttgg cgcattctta atggcttcgg caaagccttt cttacggtca atggcgacgc
    26341 tggccccaac agtgccctgc aactccacca cgttgcatgg tttgccattc acttctttta
    26401 ccagccagtc accaatcaac ttgccttcga ggatgttgtc ggcggtgaca gtggtcatat
    26461 agagagattt gtctttcaca tcgatggaac ggtcaagcaa gaagaccggg atttcggcat
    26521 ctttcgcctc ttttaatacc ggctcccaac cggtcgctac caccggagcg ataaagatcg
    26581 catccacccc ttgcgcgacg aaggaacgta ccgctttaat ctggttttcc tgcttttgct
    26641 gaccatcggc aattttcaac gtaattccgc gcttttcggc ttcacttttc gccacgttgg
    26701 tttctgcggc gcgccagccg gattccgatc cgacctgcga aaatccaacg gttaatggag
    26761 cagccaacgc aatagacgac atggctgccg agactgcaga gactacaagt aagcgtttcc
    26821 acatagggtt gtcctcgtca gggtaattat tattggtgaa aagatgttcg cgaaaaaact
    26881 atagacaatt cgttatgtaa cggattgcgt tacatcacac ttcaaaaaag taaataaaac
    26941 gttaatcaca agtttgtaat cgctttcatc tcactatgaa aaatgcggct acggttatgg
    27001 attttcctgc tctttaaacc accttaaaac tggctaaaaa ggaaaatgaa gaagaaaaca
    27061 agcgaagaca ttcggcgcga gttggctata atactcggca cttgtttgcc acatattttt
    27121 aaaggaaaca gacatgagct tactcaacgt ccctgcgggt aaagatctgc cggaagacat
    27181 ctacgttgtt attgagatcc cggctaacgc agatccgatc aaatacgaaa tcgacaaaga
    27241 gagcggcgca ctgttcgttg accgcttcat gtccaccgcg atgttctatc cgtgcaacta
    27301 cggttacatc aaccacaccc tgtctctgga cggtgacccg gttgacgtac tggtcccgac
    27361 tccgtacccg ctgcagccgg gttctgtgat ccgttgccgt cctgttggcg ttctgaaaat
    27421 gaccgacgaa gccggtgaag atgcgaaact ggttgcggtt ccgcacagca agctgagcaa
    27481 agaatacgat cacattaaag acgttaacga tctgcctgaa ctgctgaaag cacagatcgc
    27541 tcacttcttc gagcactaca aagacctcga aaaaggcaag tgggtgaaag ttgaaggctg
    27601 ggaaaacgca gaagccgcta aagctgaaat cgttgcctcc ttcgagcgcg caaagaataa
    27661 ataagttctt ctagcgtaat aaccctgaac gccgggcttc ggttagtagg ggttttttat
    27721 gcccgcgata aataaacaca ccttattcca ccaccgcctg caaacgtaat aacgcctctt
    27781 ccaccacctc acccgcagcc agaccaatac gctttgccag ccgggcgcgt agatccatca
    27841 tccgcacctg attcaccagc accacgccgt gcacatcacc ttcttcgcaa tgtaaaggaa
    27901 cgctaaatcc ggcataacgg gcaaaatttc cgccctgcgt aatgggggcc accagcgtca
    27961 ttcccagttg attaaaggct tgaacggaga gcacaagcgc aggtcgacca gcaccttgct
    28021 gttcatggcc gcttgctgga tcaaagccaa ccagcacaat gtctccccgt tcaaattcac
    28081 ttttctttac catatttcgt cacccgcagg ggtggattta ccccagacat cctgctcgct
    28141 aagttccgtg gcgttcatgt cacactgtgc cagcagttca tcaagcgagt agcgcctgga
    28201 gatgggtgtc agaatcagtt gattgttgct cacttgcgcc tccacgctct gccccggccg
    28261 taagttaagt tctttcatca caacattagg aatgaccata cctgaactgt tcccccattt
    28321 ttttatggta atacgcatct gcacctcctt aagttataca aagtataact cacggtgcta
    28381 caggaaacgt tttacccctg cgcatttgtg aacgccagca gagtttttta tgcgttaaca
    28441 gatatggaaa gcggctcgga gagaaaagca aaaggtgagg gaattacaaa acagaatgcg
    28501 agtcgtctcg caaaaaacaa cgccacccga aggtggcgta tgcatatggt tacttatccc
    28561 tgtctaacca gtcgccgctt tcaattagct ttaatccatc gacgggtcgt tgataaacgt
    28621 acatccatgc actcccgtac ggcgtctgaa tcaactggcg cgcgtattca ccgcccctgg
    28681 tgcgcaaggc atcaagttcg gccagcgtgg cgttgtcaat acgataaact tcaccgtgta
    28741 ctgttccgtt ccccggaact gcgcctggat agtggcccag gctatacaac tggtaattat
    28801 cgatactgaa atcgcccagt aactgggcat tggtcatcca gtgactgttg ccttgtttgt
    28861 ggcgtaaact gccgtagaca aatattcgca ttgctaaaac tcgaactgat agagcaaatc
    28921 cagtgcctgg tctacaccag acacggcttc catatatagc ttaggcatca ggcgataacg
    28981 taacgtgagt gttgctatag agtcaaatat acccacgccg tatttcactt gcagacctgg
    29041 caatacatag ccgctgacca ccacctggga ggagtcgcct actccctggg tgtcgagcgc
    29101 taaattgctt acgccaaacg tctcgccgat tttacccaca atctgaccac tttgtgcaac
    29161 ccccagacca atcagcatcg aggtcatcgc cgcactgtcg ctctgatcgc tctccagccc
    29221 ttgtccacgt agcaaataag acaatgcagc ttgttgcgac atggccgggt cagagaagat
    29281 ctccgctttc ggttcgtccg ccagaccagt gacgcgaact ccggcgatta cgtcgtcttc
    29341 tgtagcatcc gggttacgaa tagcttcgat attaagatac ggttgatccg gcggaccaga
    29401 gaacagtaac tcacccttac gcacaatcag atcctgacca taggcgtgga aacgcccttc
    29461 agggatgttg atctgcccgt tcaggcccag cccttgttta tcctgtacga cgttaagatc
    29521 gcccgtcagc cgcgctttca ggccaaaggc gtcaatgcgc acattgttgc cgacgtggac
    29581 aatcaggttg ctgttaatcg gaatcgacgc cgtttttgcc tcttccggtt gcaggttatc
    29641 gttaagcatc accacatcgc tggagacgcc caccgcgctt tccggcagat cgtgcaccac
    29701 gatgcgcgcc cacgggacat ccacgcgacc atcaagggta aacaggtttg gtgtagcctc
    29761 aaatacaaca tctggtgata tatccattcg taccatcggc ggcacggtga tacgcacttt
    29821 actgcccttc gctgttaccc gcgcccgcca gttttcaatc tggctccagt cggcatcacc
    29881 gttcaggtag atttcaccct gctgggtccg tactgtaccg gcaagcgtcg agcgcatacc
    29941 gttaaagttg accgcaagct ggctcggttg catatcaaac ggcataaagt tgccgtcgat
    30001 atccacaccc gtaacctgaa gctgaccaaa caactgcggg ctttgcacat caccacccag
    30061 acgcaagttg gcactcacca tccccgcagc tttttcccca cgggtaaaga tagggtttat
    30121 catcgcaagg ttgaagttac ggatattgac gttgccacca agattacggc ggccttgcgg
    30181 atcggtcacc tgcacctgtc catcaaactg gccgttattg gtcaggcgga tggtccagcc
    30241 caattcggca cggttgttac gcaattccgc cgtcagattc agcgtctgaa acgccacggg
    30301 cagcgccgca tcgttgacgg tttgcgttac ctgcacgtta cgccccgaaa gggtgatact
    30361 gccctgcggc agcccctctt tcgtggtgtc ccaggcaacg tccgctttac ccgtgaagat
    30421 accgctggcc tgagtggttt ctggcataaa cggtttcagc atggcgaggt cgaagcggtt
    30481 gagattcacc accgcacgcc cttcagcccc ggcatcaatg gtttgcggca cgcacagttc
    30541 cgcattcggg ttaagccaac agtgtggccc gatgctgatt ttttgctcct tattgcggta
    30601 atccagcgca atatcgcggg tcagcgacca cgggccgacc ggcgtctgga agcgggtatt
    30661 gctaagagtt cccttccagc gttcttcttt gcgatcaaaa cttcctgcca gattaagctg
    30721 cccggaaacc ggctcgccct gaatccgcaa ctgtaactcg tgctgttttt cgctgccttt
    30781 ggcattcagg gtgacgaggt tgatactcac atcgggctgc gagatttgct caacgcgtac
    30841 gtcaagttta cccgcaatct gatcggtgga tttgatatcg ccttccacgc gaacctgcgc
    30901 cacggaaagt tcctgccagc gcaggccgcg cgcggtgata tccgccagta gttgtggcgc
    30961 ttccaccgtg ccgcgtactt tcaccagccc tttcgctgta ccgccaagcc ccggcagcgc
    31021 gttatccagc cccggcgcgt tgatggtggc atcaagattg agatctttta cccccagctc
    31081 gcctttcact tcggcactgt ttggccccag ttccagatgc agccctggga tcttccactg
    31141 catataactg ttgcctttca gcgtaccgtc aacgttcact ttgttctgtt tgacgttacc
    31201 ggtcagcttc agttctggca cctccatctg ccaggtgccg ccgtacaggc taccgcgggt
    31261 tttaatcaat ccattgagtt tcgacggcca ctccgggaac tctttggcgg tgttaatgcc
    31321 gttaagcgtt agctcaccgc gccagctaat ggcctgctgc cagtcgagca acgccttgag
    31381 ttcagttttc ccttccagcg ccgagatggt gagtttgtcg agattgacct gctgttcatt
    31441 gcctttggca tcaagggtaa tggtggctgg cgggatctcc tgtcccttca ctgccgtacg
    31501 catagagagc gtgtaatcgg tcattttgcc ggtaagtttc agtttcagat catccgcctg
    31561 atactgctta tctccagtga atggccagta aagctgtttg ctgttcactt ccacgttgag
    31621 cggcaatccg gcttccgcca gtcgcgtctg ggcgcgtaaa tccatatcca ccggaccaga
    31681 aaggttaacg cctatctcca gctgttcgcg cagcgcgccg cccactttca gcttcacctt
    31741 ttcacctttc aacggctcca cgttcagtgt gctgttgagg gtgatatcca ccggccagtt
    31801 gtctgacagc tgcgccgtgc cgctggcgtt gacaatcccc tggttggagt cgatatccag
    31861 cgcgtccagt ttggtattgc cgtcgatact gctcactttc agcagcatgg tgcgcacggt
    31921 gatgtctgtg tcgcccgtca cgcgcagttg ctcgccctta aactcttcaa tgttcaggtt
    31981 gagcggcaga tgcacgtcgg tcatttccgg caataccggg cgagaaaaga gatctttcag
    32041 cgtttcgccg agcggctttt catccggctg cggattttca attttcggtt cgacaacttc
    32101 ttcctgcgcc acttctgcca ctttcggcaa agcaatcaac aggcctttca gcgacgtcgg
    32161 tttcagggtc agggttttct cctgccagtt caggccggag gtgaagtcca tcaccgatac
    32221 cgtggtgtca tcaatcttga tgttgacgtt atccagcgcc acccgtgtca gggtgatggg
    32281 atatggcgtg gagagatcca gcggaccgct atcttcctct tcttcaacct gttcagaagg
    32341 aggcattttt ttactgtcga tgttgacctg aatgtctttc agcgccaggt cattaataca
    32401 aacactgctg ttccacaggc actcaagccc gaccgccaga tgcagattgc ccgcttttac
    32461 cgccacgcct ggctgctcat aacgaacgtc agacaaggtg agatcgcgcc aaccgccggt
    32521 gaccttgcca atatccagtc ctagcaccca gcgatccgcc gctttaaata ccagatgcag
    32581 gccgctggtg gtgcccacca gaaacgccac cgatcccagc aacagtaaga taacgataac
    32641 cacgccgagg ctgatttttt tccataaact cataattctg gccccagacc gatgtaaaac
    32701 tgtaacccgt gttcgtcttt atccgcgacc ggtacggcaa aatcgagttt gattggcccg
    32761 accggcgatt cccagcgcac gccgaccccg gtaccggttt taaagtcgct gcggcgaata
    32821 tcgcttaccg cttcgccgct atcgacaaac accacgcccc accattttcc ggtcacgttg
    32881 tactggtact ccagcgatcc ggttatcaac ttcgaggccc ctttcaggtc accgttggcg
    32941 tatttcggag cgattgattt gtatttgtag ccgcgaatac tgcgatcgcc cccggcgaag
    33001 aaacgcagat ccggcggtac tttgtcgaaa tcaccggttt caatccagcc cagcgtgcca
    33061 cgtgtaacaa aacgatggcg atcgtacagt gtgcggatcc agacgttctg cgcctggaaa
    33121 acggagaaat cgacatctga gccccaggcc gtgttggagt agtcgataga gtagcgttgc
    33181 gagtcgcccc aggttggcat caggccacca cgagaacgcg tgcggctaat catcacccca
    33241 ggataaaaca gcatcgtggt gttggtaatt tcaccctggg taaagtgatc gaggctccag
    33301 cgcaggttaa tggcacgctg ccagccgctg gagagatccc agtagcgaga agctaccagc
    33361 gtagtggagt cagactcggt atcgttcagg tcagtgcgct taaaaccgcc ctgcaccaga
    33421 taatattgtt ccagcggatt cttcagcagc ggcattttat agctgaagtc gaggatctgt
    33481 tctggcgcgg aaatactggt actggtggtc aaactgtgac cgtaagagtt catccacggc
    33541 tttttccacg tcgctttcac gcgcggtccc acgtccgtag agtaaccgac gccggtttcg
    33601 atggtgtttt ctgttcgcgg cgaaaccacg cccgtcaatg gtaatacttt cgtttcgcgc
    33661 gctttatcaa attgtggagc caccaccacc gagttaaacc agccggtagc agaaagtcgg
    33721 cggttcagtt ccgccagatc tttcgattcg tactcatcgc cctctttaaa cggcaccaga
    33781 ttttgcaggt attcatcgcg gatttgtgat ccttcaaagg tcacatgccc aaagcggtaa
    33841 cgttcgccac tgttataatc aatatcccag aaggctttat gcaggccgag cgcaatgccc
    33901 agctgcgctt tggtaaattc gctatcgaaa taacctttac gcaacgcaat gctggttaag
    33961 gactttttga aattttcata atcgccctgg ttcagcaccg tgccaatagc cgggcgagta
    34021 tcgagcaatt tcaaatagtc tttatcggtc cgcgcgccgc cgcgcaatac cacatcggtg
    34081 ccgccaatta acaccggcac gcctggcgtg actttggcga tcaatacctg ccgccctttc
    34141 tttggcggtg gacggagatc aaattcaatg gtcggctggt aataacccag cgctttcaga
    34201 ccttcgcgga tggcatcatc gacgcgtgcg cgaaagcgac ggtctggcgt cacttcatca
    34261 ctttcaatcg tagaaagctg cgcacgaacg ttcttttcca gctgtcccga taacccctcg
    34321 acctgtagac ggacgttcgc ggcgacggca gatccgctta agcagagtaa gcttacacag
    34381 cataactgtc ggatatagcg cacattttct cctgaatatc cttttttcct gcccctggaa
    34441 cgccgttaaa cggcttaaca aaaatccagt aatatggatt aaaaagcaga ctaaacccca
    34501 aatatttctt atgttttact ttagacctat tcacggtggt tattgtgtgc aaatacgcct
    34561 cttgttacaa ccttaacccc aatgaccgat tttcgggaga gcgacaccat gagtttattt
    34621 gataaaaagc atctggtttc ccccgccgat gccctgcctg gacgtaacac cccgatgccc
    34681 gtagccacgc tgcatgcggt caacggtcac tcaatgacca atgtacctga cggaatggag
    34741 attgccattt ttgcgatggg ttgtttctgg ggtgtggagc gtctgttctg gcagttaccc
    34801 ggcgtttaca gcaccgccgc aggctatacc ggaggctata cgccaaatcc gacttatcgg
    34861 gaagtgtgct ccggtgatac gggtcatgcc gaagcggtac gcattgttta cgatccttcc
    34921 gtcatcagct atgagcagtt gctacaggta ttttgggaga atcacgatcc cgcccaggga
    34981 atgcgtcagg gcaatgacca cggcacgcag tatcgttcag cgatttatcc gctgacccca
    35041 gaacaggatg ccgcagctcg cgccagtctg gaacgttttc aggcggcgat gcttgccgcc
    35101 gatgacgatc gtcacatcac cacggaaatc gctaacgcca caccgtttta ttatgccgaa
    35161 gatgaccacc agcaatatct gcataaaaac ccgtatggtt actgtggaat tggcggaatt
    35221 ggcgtctgtc tgccaccgga agcatagcgt tacgggtaca aatgtagatt gttgataaag
    35281 tgcgctttgt ttatgccgga tgcggcgtaa acgccttacc aggcctacaa aatcgtccaa
    35341 attcaatata ttgcaaggac tgcgtaggcc tgatcggcat agcgcatcag gcagtattgc
    35401 atttatcagc agtctgaatt ttaaccctct ggcgacttta cagtacctta cgctatacta
    35461 gccactgaaa atgccggttc actttcttcg aatcggcttt caatgtgtat ttcacacaaa
    35521 ttaatcaact tccccttccg aggatctggc ctgaaaggtc ggataagata tgttaaacag
    35581 tattttagtc atactctgct tgatcgctgt aagtgcgttc ttctcgatgt ccgagatctc
    35641 gcttgccgcc tcacgcaaaa tcaaacttaa actgctggct gatgaaggca atataaatgc
    35701 ccaacgcgtt ctgaatatgc aggaaaatcc cggcatgttc tttaccgtgg tccaaatcgg
    35761 tctgaacgca gtggcgattc tcggcggtat cgtcggtgat gcggcatttt ctccaacttt
    35821 tcacagcctg ttctcccgct atatgtcggc agagctctct gagcaactga gctttattct
    35881 ctctttctcg ttagtgactg gcatgtttat cctgtttgcg gatttaaccc cgaaacgcat
    35941 cggtatgatt gcgccagaag cggtagcttt gcgtatcatc aacccgatgc gcttctgcct
    36001 gtacgtatgc accccgctgg tgtggttctt caacggcctg gcgaacatga tcttccgtat
    36061 tttcaaactg ccaatggtac gtaaagatga catcacttct gatgacatct acgcggtagt
    36121 ggaagccggt gcgctggcgg gcgtgttacg taaacaggaa cacgagctga ttgaaaacgt
    36181 ctttgagctg gaatcccgta ccgttccgtc ttcaatgaca ccgcgtgaaa acgtgatttg
    36241 gtttgatctc cacgaagatg agcaaagcct gaagaataag gtggcggaac atccgcactc
    36301 taagttcctc gtctgtaatg aagatattga ccacatcatc ggttatgtcg attctaaaga
    36361 cctgctgaac cgcgtgctgg ctaaccaaag cctggcactg aacagcggcg tacaaattcg
    36421 caacacgctg attgtgccgg atacgttaac cctttcagag gcgttggaaa gttttaaaac
    36481 cgcaggtgaa gacttcgcgg tgatcatgaa cgagtacgcg ctggtggtgg ggatcatcac
    36541 cctcaacgac gtgatgacca cgctgatggg cgatctggtc ggtcaggggc tggaagagca
    36601 gattgtcgcc cgtgatgaga actcatggct gattgacggc ggcaccccaa ttgacgacgt
    36661 catgcgcgtg ctggatattg acgagttccc gcagtcgggc aactacgaaa ccatcggcgg
    36721 ctttatgatg tttatgctgc gtaagatccc gaaacgcacc gattcggtga aattcgccgg
    36781 ctacaaattt gaagtggtgg atatcgataa ctaccgcatc gaccagctgc tggtgacccg
    36841 gatcgacagc aaggccaccg ccctttcgcc aaaactgcct gacgctaaag ataaagaaga
    36901 aagcgtcgcg taacccccag aaacatcaac ggctcctgaa tcaggagccg tcttattact
    36961 gcatagcact ttggttaagc catctctgtt tgcagacgca taacctgacg gttaacttcg
    37021 gacatcactg acaaatgcag cttatccttc acttttggga taagaatctt acccttatca
    37081 aattcaaaag cgccaacgtc cttgatgtat aaccgtccac ggaacaggat ctttacgtac
    37141 ttcgccacct gaagtgggtt gtaccgttgg aaaattttca ttgttatctc ctgctgagta
    37201 ttacgcctgt gcggtgccac aatcggccca actattatga ggcgcaaatt ttaatgctta
    37261 gtgactatag actatccggg caatgtttcc accgcgtata acttttttta cctaaaggtt
    37321 acaattattc agaattatct ttttaccgaa gcgcggtctt cagtataagc attcattttt
    37381 catatgattt gtgcgcttga ccgcaaactg gcatcacact tgcgggaaat tcgataaata
    37441 gcacatatga ttaaaactca gacccaagtg gtcggatcac ctgcatatca taagaaggaa
    37501 acaccatgac cctacgcaag attctggcac tcacctgcct gctgttgccg atgatggctt
    37561 ccgcacatca gttcgaaacc ggtcagcgag tgccgccgat tggcattacc gatcggggcg
    37621 agttggtgct tgataaagat cagtttagct acaaaacctg gaacagcgcg cagttagtgg
    37681 gaaaagtgcg agtactgcaa catattgctg gtcgcacctc tgcaaaagag aaaaacgcga
    37741 cgctgattga agcgattaaa tcagcgaagt taccgcacga tcgttaccag accaccacca
    37801 ttgttaacac cgacgacgcg attccgggtt ccggcatgtt tgtgcgcagc agtctggaga
    37861 gtaataaaaa gctttatccc tggtcgcagt ttattgtcga tagcaatggc gtcgcgcgcg
    37921 gtgcctggca gctggatgaa gaaagttccg ctgtagtggt gctggataag gatggtcgcg
    37981 tgcaatgggc caaagacggg gcgctcacac aggaagaggt gcagcaagtg atggacctgc
    38041 tgcaaaagtt gctcaaataa tggcaataac ataaattctg caaaaataat tttcccctat
    38101 cgttatctat gggatagggg aattaagtct cttagttctt acgccgaact catactggta
    38161 acgctagttg ctctttttta aatgcctcaa tggtcgattt atatttctcg caaatctttt
    38221 ctttggtaat attactatct gcggccagat tcgcacttac tatcgcatcc acttgttcgg
    38281 ctaattcgac ttgttggcaa cctgcattaa ccagaaaatt ataaataaga gagtaatctt
    38341 catcctccaa aacaacagag gaaccattac gaaaacgttc atcccctcca tagattttaa
    38401 gcgcaaagcc caaagattct atgactatgg caaaattatc atcaattatt ttttcgtaaa
    38461 tataccatcc atcgcattct ttaattccac agcctggtat ttggagttca aatttacccc
    38521 gttttaattt gcttactata tcctccaata aggcataacc gaaagatacg tcaccaaacg
    38581 cctggcgaat ataaccttta atcctttttg gtatgtcaga ctgttttttc tgcaaaatat
    38641 cacaggcata caatcttctg ttttgttcta acggaaactc gcggtttttg aaatccatga
    38701 atgagttata ctcatttatt gttatgggaa tttcttcgta ttcaaatcca ctgcctcgca
    38761 atgtcattgt atattcggca ccgttatctt taaggctgaa actgataaga agcttatcca
    38821 tggctttaac agtttcattt tttgatgaaa gcacttgata actatcttta tcaatctgag
    38881 ttgcgcaacg aaaaaactcc cacaaagtta acaggctatc a
//
LOCUS       DAJSKJ010000016        36741 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000016 DAJSKJ010000000
VERSION     DAJSKJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.016"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..424)
                     /locus_tag="ON345_000734"
     CDS             complement(<1..424)
                     /locus_tag="ON345_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070843.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVE"
     gene            complement(439..3102)
                     /gene="aceE"
                     /locus_tag="ON345_000735"
     CDS             complement(439..3102)
                     /gene="aceE"
                     /locus_tag="ON345_000735"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR3070844.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(3263..4027)
                     /gene="pdhR"
                     /locus_tag="ON345_000736"
     CDS             complement(3263..4027)
                     /gene="pdhR"
                     /locus_tag="ON345_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR3070845.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            4568..5941
                     /gene="aroP"
                     /locus_tag="ON345_000737"
     CDS             4568..5941
                     /gene="aroP"
                     /locus_tag="ON345_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR3070846.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(5984..6838)
                     /gene="ampE"
                     /locus_tag="ON345_000738"
     CDS             complement(5984..6838)
                     /gene="ampE"
                     /locus_tag="ON345_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR3070847.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(6835..7386)
                     /gene="ampD"
                     /locus_tag="ON345_000739"
     CDS             complement(6835..7386)
                     /gene="ampD"
                     /locus_tag="ON345_000739"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR3070848.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            7474..8367
                     /gene="nadC"
                     /locus_tag="ON345_000740"
     CDS             7474..8367
                     /gene="nadC"
                     /locus_tag="ON345_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR3070849.1"
                     /translation="MPPRRYNPDTRRDELLVRINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINTNQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            8570..9010
                     /gene="ppdD"
                     /locus_tag="ON345_000741"
     CDS             8570..9010
                     /gene="ppdD"
                     /locus_tag="ON345_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR3070850.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            9020..10405
                     /gene="gspE"
                     /locus_tag="ON345_000742"
     CDS             9020..10405
                     /gene="gspE"
                     /locus_tag="ON345_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCR3070851.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            10395..11597
                     /gene="hofC"
                     /locus_tag="ON345_000743"
     CDS             10395..11597
                     /gene="hofC"
                     /locus_tag="ON345_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR3070852.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCLYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(11632..12675)
                     /gene="guaC"
                     /locus_tag="ON345_000744"
     CDS             complement(11632..12675)
                     /gene="guaC"
                     /locus_tag="ON345_000744"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR3070853.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(12831..12875)
                     /locus_tag="ON345_000745"
     CDS             complement(12831..12875)
                     /locus_tag="ON345_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070854.1"
                     /translation="MIIKTLKMSARKRN"
     gene            12900..13520
                     /gene="coaE"
                     /locus_tag="ON345_000746"
     CDS             12900..13520
                     /gene="coaE"
                     /locus_tag="ON345_000746"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR3070855.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            13520..14263
                     /gene="zapD"
                     /locus_tag="ON345_000747"
     CDS             13520..14263
                     /gene="zapD"
                     /locus_tag="ON345_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR3070856.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            14273..14470
                     /gene="yacG"
                     /locus_tag="ON345_000748"
     CDS             14273..14470
                     /gene="yacG"
                     /locus_tag="ON345_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR3070857.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(14570..14959)
                     /gene="mutT"
                     /locus_tag="ON345_000749"
     CDS             complement(14570..14959)
                     /gene="mutT"
                     /locus_tag="ON345_000749"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR3070858.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(15019..17724)
                     /gene="secA"
                     /locus_tag="ON345_000750"
     CDS             complement(15019..17724)
                     /gene="secA"
                     /locus_tag="ON345_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR3070859.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(17786..18298)
                     /gene="secM"
                     /locus_tag="ON345_000751"
     CDS             complement(17786..18298)
                     /gene="secM"
                     /locus_tag="ON345_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR3070860.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(18529..19446)
                     /gene="lpxC"
                     /locus_tag="ON345_000752"
     CDS             complement(18529..19446)
                     /gene="lpxC"
                     /locus_tag="ON345_000752"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR3070861.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(19547..20698)
                     /gene="ftsZ"
                     /locus_tag="ON345_000753"
     CDS             complement(19547..20698)
                     /gene="ftsZ"
                     /locus_tag="ON345_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR3070862.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(20759..22021)
                     /gene="ftsA"
                     /locus_tag="ON345_000754"
     CDS             complement(20759..22021)
                     /gene="ftsA"
                     /locus_tag="ON345_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR3070863.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(22018..22848)
                     /gene="ftsQ"
                     /locus_tag="ON345_000755"
     CDS             complement(22018..22848)
                     /gene="ftsQ"
                     /locus_tag="ON345_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR3070864.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(22850..23770)
                     /gene="ddlB"
                     /locus_tag="ON345_000756"
     CDS             complement(22850..23770)
                     /gene="ddlB"
                     /locus_tag="ON345_000756"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3070865.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(23763..25238)
                     /gene="murC"
                     /locus_tag="ON345_000757"
     CDS             complement(23763..25238)
                     /gene="murC"
                     /locus_tag="ON345_000757"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR3070866.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYTEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDAILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(25292..26359)
                     /gene="murG"
                     /locus_tag="ON345_000758"
     CDS             complement(25292..26359)
                     /gene="murG"
                     /locus_tag="ON345_000758"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR3070867.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(26356..27600)
                     /gene="ftsW"
                     /locus_tag="ON345_000759"
     CDS             complement(26356..27600)
                     /gene="ftsW"
                     /locus_tag="ON345_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR3070868.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(27600..28916)
                     /gene="murD"
                     /locus_tag="ON345_000760"
     CDS             complement(27600..28916)
                     /gene="murD"
                     /locus_tag="ON345_000760"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR3070869.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(28919..30001)
                     /gene="mraY"
                     /locus_tag="ON345_000761"
     CDS             complement(28919..30001)
                     /gene="mraY"
                     /locus_tag="ON345_000761"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR3070870.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(29995..31353)
                     /gene="murF"
                     /locus_tag="ON345_000762"
     CDS             complement(29995..31353)
                     /gene="murF"
                     /locus_tag="ON345_000762"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR3070871.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(31350..32837)
                     /gene="murE"
                     /locus_tag="ON345_000763"
     CDS             complement(31350..32837)
                     /gene="murE"
                     /locus_tag="ON345_000763"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR3070872.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQVIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(32824..34590)
                     /gene="ftsI"
                     /locus_tag="ON345_000764"
     CDS             complement(32824..34590)
                     /gene="ftsI"
                     /locus_tag="ON345_000764"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR3070873.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(34606..34971)
                     /gene="ftsL"
                     /locus_tag="ON345_000765"
     CDS             complement(34606..34971)
                     /gene="ftsL"
                     /locus_tag="ON345_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR3070874.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(34968..35909)
                     /gene="rsmH"
                     /locus_tag="ON345_000766"
     CDS             complement(34968..35909)
                     /gene="rsmH"
                     /locus_tag="ON345_000766"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR3070875.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(35911..36369)
                     /gene="mraZ"
                     /locus_tag="ON345_000767"
     CDS             complement(35911..36369)
                     /gene="mraZ"
                     /locus_tag="ON345_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR3070876.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         8648 a        10262 c         9093 g         8738 t
ORIGIN      
        1 cttctacggt gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt
       61 cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgccgcag
      121 ccgcgggtgc tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt
      181 ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc
      241 caacagagac tttgatctct ttaacgatac ccgcctgcgg agacggaact tccatagagg
      301 ctttgtcgcc ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga
      361 ccaggatctc ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag
      421 ccattattct tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa
      481 tttggcgatt gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc
      541 cagcgccgca accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg
      601 gctgtcggaa cgaccgaagc catcagtacc cagtacgcgg tagtcgtcag ccggtacgta
      661 agtacggacc tgctcagcga acagtttcat atagtcggta gatgccactg ccggagcgtc
      721 gttcatcacc tgagcgatat acggaacgcg cggagtttcc agcgggtgca gcatgttcca
      781 gcgttcacaa tcctgaccat ctcgcgccag ttcggtgaag gaggtcacgc tataaacgtc
      841 agaacctacg ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat
      901 agaaccggag cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta
      961 gataccttta cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc
     1021 gttcagcgtg gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag
     1081 accgtcatgc ataatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt
     1141 cggaatagtc agcgactgaa tgtggctgtg accatcttcg tgctgcagac cttcgccgtt
     1201 cagggtggta cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtcgccagc
     1261 cgcccagcac agatcgccaa tacgctggaa gccgaacatc gagtaataga tgtagaacgg
     1321 gatcatcggc agattgttgg tgctgtaaga ggtcgccgct gccagccagg aacaacctgc
     1381 gcccagctcg ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc
     1441 aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg
     1501 acggaacaga ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg
     1561 atctttgatc gacttgttct tcagcattac gttcagggca cgaacgaaag cgatagtggt
     1621 agagatctct ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc
     1681 aagcttctcg gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg
     1741 tgcgtgcaga taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc
     1801 gatatctgca tcagacaccg gcacattgaa acggtcgcgg atgtgacgca cgccgtccat
     1861 gttcattttc ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta
     1921 acctttaatg gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt
     1981 gaatgcagcg tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc
     2041 gtcagtccag tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg
     2101 aacgtacgca ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat
     2161 cagctggatc agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca
     2221 catcactttg atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat
     2281 cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaagac
     2341 caggttatcc agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc
     2401 catttcaccg tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg
     2461 gtgttccaga tatttcagga atttagcctg gtaaatagca ccaatcggcc ccagacccat
     2521 agatacggtc gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag
     2581 gccattgccg tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc
     2641 caggaaagca cgagcgtaca cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc
     2701 gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc
     2761 ggaagactgg aaggacgcca tatggccgcc cagttcgagg tcttttttcg acgcacgcag
     2821 caccgtcatg atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt
     2881 acccggatac tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgatacctgt
     2941 gcctgcggct acgtttacac cgcctttgcg ggcttcagca agcagttggt cgatcagata
     3001 ctgagcacgc tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg
     3061 agtttcgatc ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat
     3121 ctaataacgt tgagttttct ggaacctgtc ccattgaact ctcgccggaa agctcaataa
     3181 gacaggttct acgtttagtt gccgcgcttt tatatgcgct tgatttacaa catcttctgg
     3241 ataattttta ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct
     3301 cacggcggct ctcttcacga cttctgtcga gcaaaatttc ttcgataaag gccagatggc
     3361 gatgcgatgc ttcgcgcgct tcttccggct taccggccat aatcgcttca aatatgcggg
     3421 tgcggtgact actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct
     3481 ggcggacatt ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat
     3541 tgtgggccgc ttcagtgaca gcaatctgat actggagtac ggcgtttgat tccgcgtcca
     3601 gatcgccaga ctgctgcgcc agttctatgg cgtgatggag ttcacggatg cgttccttgt
     3661 cttcatcggt actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg
     3721 tttcgagcaa gtcatactgt gactcaggat ggtcggagag cagctccacc agcggatcgc
     3781 tgaagctttg ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca
     3841 agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcaaact
     3901 gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga
     3961 tcaaaaactc cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt
     4021 aggccatgag ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg
     4081 tctgtgacgc taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc
     4141 atgaaactgt gcacatttta acaacttgac atatataacg tttcaaagtt gtaactatgc
     4201 acaaatgtag actttacgta ggaaaggagt tttttaacga ttattaaact ataaaaatcc
     4261 gaattgaacc gattcactta ccaattttgt gatttttaat tcaattaaaa cgaatttaaa
     4321 ttcattctac atattgagag gggttgaggc tgagctttac aaacggtttc tttttaagca
     4381 actcatcttc aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat
     4441 ctgacggaag tctttttgta acaattcaaa cttctttgat gtaaacaaat taatacaaca
     4501 aacggaattg caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg
     4561 aggtttcatg atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg
     4621 ccatattcag cttatcgcgc tgggtggcgc gatagggaca gggctattcc tgggtagcgc
     4681 ctccgtaata cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat
     4741 cgcctttctg atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc
     4801 ctttagccac tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa
     4861 ctactgggta ctgtacgttt tagttgccat ggcagagctg actgccgtgg gtaaatacat
     4921 tcagttctgg tatccggaaa tcccaacctg ggtttctgcc gccgtattct ttgtggtgat
     4981 taacgccatc aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat
     5041 tatcaaagtt attgcggtag tagcgatgat catcttcggc ggctggctgc tgttcagtgg
     5101 caacggcggc ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt tcctgccgca
     5161 cggcttcacc ggactggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga
     5221 actggtgggg atcaccgcag cagaagctga taacccggag caaagtatac cgaaagcaac
     5281 taaccaggtt atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc
     5341 actgatgccg tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga
     5401 gttaggcgat acctttgtgg cgaatgcgct gaacatcgtg gtactgactg cggcgctctc
     5461 cgtgtacaac agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg
     5521 taatgcgcca aaagcgctgg cgtctgtcga taaacgtggt gtaccagtaa ataccattct
     5581 ggtgtctgca ctggtaacgg cgttgtgcgt actgattaac taccttgccc cagagtccgc
     5641 tttcggactg ttaatggcgc tggtggtatc tgcactggta atcaactggg cgatgattag
     5701 cctggcgcat atgaaattcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc
     5761 tgctctgctt tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt
     5821 gattatgctg atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat
     5881 cgtgttaggt atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta
     5941 atctctctac gccctcaccc gtacagggtg agggcaataa tctttacacc aacgccccgt
     6001 aaatcgtcag tagtgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa
     6061 ccgctgccct cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg
     6121 ccagacgcgt taacacctga tactgcgaag tatggaaatc acccagcgaa gcaaaccagg
     6181 ccggtaacgc tttctcacca tgaccgatca aggcatatac cacacccgca agacgaaccg
     6241 gcacccaatc cagtacatga agcacggcat caatgccgga ctgtaaacga tgatgcggcg
     6301 tctgatatcg cgccagccag tattgccatg cacgcaaaaa cgcatacccc atcagcgtaa
     6361 cgggtcccca ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt
     6421 taatccacag caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg
     6481 ggacgccgtg aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac
     6541 gtgaagcagc tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca
     6601 gcaaaccaat cagcagccac accagtagcg tgggaacgtt gaacaatact ccctgcaatg
     6661 cgcgtaacag taaaaaagtc acgcccatcg caataatggt catgcctaac gtgcgcccga
     6721 gagaaaaatg tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct
     6781 cgcccaactt aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt
     6841 tgtctccttg ctgaccagca cacgaaaccg tgcccaatca aatgcagggc cgggatcggt
     6901 tttccgatcc ggcgcaatat cacaatggcc cgtcatgttt ttagcgatat ccggatagca
     6961 atcaatcagt gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag
     7021 cgtatcggtg ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cgcgcccctg
     7081 atactgagag actcccgcat gccatgcacg tttatcgaaa ggaacatact ggactatttc
     7141 accatcacgg cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa
     7201 aggatgtgcc tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa
     7261 ctcgcctggc ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccgg
     7321 gcggcaatcg taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa
     7381 caacatgcag gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta
     7441 ccttatgatt cgttagctat ctggagtttt aacatgccgc ctcgccgcta taaccctgac
     7501 acccgacgtg acgagctgct ggtacgcatt aatctcgata tccccggcgc ggtggcccag
     7561 gcgctgcggg aagatttagg cggaacagtc gatgccaaca atgatattac ggcaaaactt
     7621 ttaccggaaa attctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtcttttgc
     7681 ggcaaacgct gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc
     7741 tggcatgtgg atgacggcga tgtcatcaat accaatcaat ccttgttcga acttgaaggc
     7801 ccatcccgcg tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca
     7861 ggagttgcca gtaaggtacg ccactatgtc gaattgctgg aaggcaccaa cacgcagttg
     7921 ttggatacgc gcaaaacctt acccggcctg cgttcagctc tgaaatacgc ggtactttgc
     7981 ggcggcggag cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat
     8041 attattgcct ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcacccggat
     8101 gcgccagtag aagtcgaagt agagaatctg gaagaacttg atgaagccct gaaagcagga
     8161 gccgatatca tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc
     8221 accaacggca aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgtgaa
     8281 tttgccgaaa cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca
     8341 ctcgaccttt caatgcgttt tcgctaatag ttgacagatt tatccgctcc atcgcgggcg
     8401 gataatctcc ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg
     8461 caacgaaata aaaaacctcg gaagctgctt tcccgtttcg ttttttgccc ctcgcttgtg
     8521 ctgatggcgt ttgccaaagt agcgccaacc aaatcaagga gcgaaacaga tggacaagca
     8581 acgcggtttt acacttatcg aactgatggt ggttattggc atcattgcca ttttaagcgc
     8641 cattggtatt cccgcttatc aaaactacct gcgcaaagct gcactcaccg acatgctaca
     8701 aacctttgtg ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga
     8761 tacctgcgac ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc
     8821 catgagtgtg gcaaaaggcg tggtgtcgct gaccgggcaa gaaagtctca atggactaag
     8881 cgtcgtcatg acaccgggtt gggataacgc aaacggcgtc accggctgga cgcgcaactg
     8941 caatattcaa agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga
     9001 cgccaactaa ggagcggcaa tgaatattcc acagctcact gccctgtgtc tgcgttatca
     9061 tggagtcttg ctggatgcca gcgaagaggt ggttcatgtt gcggtagtcg atgcaccttc
     9121 gcatgagcta ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg
     9181 gacgcgccaa caaatggaag gtcacgccag tcgcacacaa cagacattgc ccgtagctgt
     9241 tcaggagaag catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga
     9301 acaacgcgcg tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg
     9361 tatcgacggc gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac
     9421 cgccagatta aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg
     9481 gcaattcact gtcgaactgg caggaaacgc cgtctcattt cgtattgcga ccttaccatg
     9541 tcggggtggt gaaaaggtgg tattaaggtt gttacagcag gtgagtcagg cactggatgt
     9601 caacacgctt ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc
     9661 acagggactg gtgctggtaa ctggccctac cggcagcggc aaaacggtca cgctttatag
     9721 tgccctgcaa aagctgaata ccgctgacat taatatttgt agcgtcgaag atccggttga
     9781 gatccccata gccggactaa accagacgca aatccatccg cgtgcaggac tcacctttca
     9841 gggcgttttg cgtgcgttat tgcgccagga tcctgacgtc atcatgatcg gagagatccg
     9901 cgatggcgaa acagcagaga tcgctattaa agcggcgcaa actggtcacc tggtgttgtc
     9961 taccctacac actaattcca cctgcgaaac gctggtacgt ttacagcaaa tgggagtcgc
    10021 ccgctggatg ctctcatcag cgcttacgct ggtaatagcc cagcgtctgg tacgtaaact
    10081 ttgcccacat tgtcgccagc agcaagggga gcccatccat attccagtca atgtatggcc
    10141 gtcgccgctg ccccactggc aggcacccgg ttgtgtacat tgctaccacg gtttttatgg
    10201 tcgtacggcc ttatttgaag ttctgcccat aacgccggtc attcgtcagc ttatttccgc
    10261 taataccgac gttgaatcgc tggaaacgca cgcacgacag gcgggtatgc gtacgctttt
    10321 tgaaaacggc tgcctggccg tggagcaagg cttaaccacc tttgaagagt taatccgcgt
    10381 actggggatg ccgcatggcg agtaagcaac tctggcgctg gcatggcata accggcgacg
    10441 gcaatgcgca agatgggatg ctatgggcag agagccgtgc tttgctgctc atggcactac
    10501 agcaacagat ggttacccca cttagcctga agcgaatcgc catcaattct gcgcagtggc
    10561 gaggagataa aagcgcggaa gtcattcatc aactggcgac gctactcaaa gccgggttaa
    10621 cgctttctga agggctggca ctgctggcgg aacagcatcc cagtaagcaa tggcaagcgt
    10681 tgctgcaatc gctggcgcac gatctcgaac agggcattgc tttttccaat gccttattac
    10741 cctggtcaga ggtatttccg ccactctatc aggcgatgat ccgcacgggt gaactgaccg
    10801 gtaagctgga tgaatgctgc tttgaactgg cgcgtcagca aaaagcccag cgtcagttga
    10861 ccgacaaagt gaaatcagcg ttacgttatc ccatcatcat tttagcgatg gcaatcatgg
    10921 tggttgtggc aatgctgcat tttgttctgc cggagtttgc cgctatctat aagaccttca
    10981 acaccccact accggcacta acgcagggga tcatgacgct ggcagacttt agtggcgaat
    11041 ggagctggct gctggtgttg ttcggctttc tgctggcgat agccaataag ttgctgatgc
    11101 gccgaccgac ctggcttata gtgcggcaga aattgctgtt acgcatcccg attatgggtt
    11161 cactgatgcg gggacaaaaa ctcacgcaga tctttacgat tctggcgctg acacaaagtg
    11221 caggcattac ttttttgcag ggcgtagaga gcgtcagaga aacaatgcgc tgcctgtact
    11281 gggtgcaact tctgacacaa atccagcacg atatcagtaa cggtcatccc atctggctgg
    11341 cgctaaaaaa taccggggag tttagcccgc tctgtttgca attagtgaga acaggagagg
    11401 catccggctc gctggacctc atgttagaca acctcgccca tcatcatcgg gataacacaa
    11461 tggcgctggc ggataacctc gcagccttac tggaaccggc gttgctgatc ataacgggag
    11521 gaattatcgg tacgctggtg gtggcgatgt atctgccaat tttccattta ggcgatgcga
    11581 tgagtgggat gggataacgc tggcgtgttg ctccacacca gcgttggaag attacaggtt
    11641 gttgaagatg cggttttctt gttcctgcac acggataaat gtggtgcgct tggtcagttc
    11701 tttcaggcgt gaagccccaa cgtatgtaca agctgaacgc aggccgccca aaatatcacg
    11761 cgcggtattt tcaaccgggc ctcgcagcgg cagcttaacg gttttacctt ctgctgcgcg
    11821 atattccgca acgccgccaa cgtgacgttt catcgcagac tcggagctca tgccgtagaa
    11881 cagcataaat ttctcgccgt tctcctcaac gatgcgaccg ccgctctctt cgtggcccgc
    11941 cagcatgccg ccaagcatga cgaaatcggc accgccgcca aaggcttttg cgacatcgcc
    12001 cggcgtggtg cagccaccgt cgctgacgat cattccgccc agaccgtgcg ccgcatcggc
    12061 acattcgatt accgcagaaa gctgcggata accgacgcct gttttgacgc gagttgtaca
    12121 aacagaacct gggccaatgc caactttaac gatatcagca ccggagagga taagctcctc
    12181 acacatttca ccagtcacta cgttgccagc acaaatggtt ttggtcggcc acgcttcacg
    12241 cgctttcgca acgaactgca cgaagtgttc ggaataacca ttcgccacat caatacaaac
    12301 gaagtttaat gccgggttca ggtcgagaat ctgtttagtt ttttcgaaat ccgcatcaga
    12361 cgtaccggta gaaaccatca catgtttcag cacatcagcg gaagaattgt tgataaacgc
    12421 ttgccactct tcgacagaat agtgtttatg cacagcagtc aaaatatcaa aagaagccag
    12481 cgcagaggcc atagaaaatg tacctacggt gtccatattt gcggcgataa tcggcacgcc
    12541 ggaccagctc tgacctgaat gtttgaaggt gaattgacgt tccagttcaa catcggaacg
    12601 gcttttaaga gtggagcgtt tagggcggat gagaacgtct ttaaaaccta acttcagatc
    12661 ttcttcaata cgcatgtgcg gattcctggg gttaatggct ataagggtaa gagcgcaact
    12721 ccagtgacgc tatcatacgc actaatcaat gcggcgcaag actgcgaaat tgtctttttt
    12781 tacgctacaa tcccataaat ttacctggta aatagtgagc gaacgtttca tcaatttcgc
    12841 tttcgtgcac tcatttttaa cgttttgatt atcatgatga attcccggga atataaatta
    12901 tgaggtatat agttgcctta acgggaggca ttggcagtgg caagagtacc gttgccaatg
    12961 cgtttgctga tctcggaatt aacgtcattg atgccgatat tattgcgcgt caggtggttg
    13021 aaccaggtgc acctgcgcta catgccattg ctgatcactt tggcgctaac atgattgctg
    13081 ctgatggaac attgcagcgc cgggccttgc gcgagcggat cttcgccaac ccggaagaga
    13141 aaaactggct taacgccctg ctgcatccgc tgattcagca agagacgcaa caccagatcc
    13201 agcaagctac ttccccctat gtactgtggg ttgtgccatt gctggtagaa aactcactgt
    13261 ataaaaaagc gaatcgagtg ctggtggtgg atgtcagccc agaaacgcaa cttaagcgca
    13321 ccatgcagcg cgatgatgta actcgcgagc atgtcgaaca aatccttgct gctcaggcaa
    13381 cgcgcgaagc ccgccttgcc gtggcagatg acgtcattga taataacggc gcaccggatg
    13441 ctatcgcatc ggatgttgcc cgcctgcacg cacactattt gcagcttgcg tcgcagtttg
    13501 tctcacagga aaaaccgtaa tgcagaccca ggtccttttt gaacatccac taaatgaaaa
    13561 aatgcgtaca tggctgcgca ttgagttttt gattcagcaa ctcaccgtta atttacccat
    13621 cgttgaccac gctggcgcgc tgcatttctt ccgtaatgtc agtgaattac tggatgtttt
    13681 cgagcgcggc gaagtccgca ctgagctgtt gaaagaactt gaccggcagc aacgtaaact
    13741 ccagacctgg attggcgtgc ctggcgtgga ccagagccgt attgaagcat taattcagca
    13801 gttaaaagcg gcggggagcg tattaatttc cgcgccgcgt atcgggcaat ttctgcgtga
    13861 agatcgtttg attgctctgg tgcgtcagcg actgagcatc ccaggcggct gttgcagctt
    13921 tgatttacct acattgcaca tttggctgca tctaccccag gcgcagcgcg acagccaggt
    13981 agaaacctgg attgccagcc tgaacccgct cacccaggca cttaccatgg tgctggattt
    14041 aattcgccag tcggccccct tccgtaaaca aaccagcctg aatggttttt atcaggataa
    14101 cggtggcgat gccgacttgc tgcgcctgaa tctgtcgctc gattcacagc tttatccgca
    14161 aatttccgga cataagagcc gttttgccat tcgttttatg ccgctggaca ctgaaaacgg
    14221 acaggtaccg gaacgtctgg atttcgaact ggcctgttgc taaggagtaa aaatgtcaga
    14281 aactattacg gtgaattgcc caacctgcgg gaaaacggtg gtgtggggtg aaatcagccc
    14341 gtttcggcca ttttgctcca aacgttgtca gctgatcgac ctcggagaat gggctgctga
    14401 agaaaaacga atccccagca gtggcgatct ttccgaaagc gatgactgga gcgaagagcc
    14461 aaagcagtga catttgtctg atgccgcacg taggcctgat aagacgcgga cagcgtcgca
    14521 tcaggcatct tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc tacagacgct
    14581 taagcttcgc aattaccggt tcattggctg gcggaaaatc atcggcatta agaccgacta
    14641 gcgacatcca ctcaccgggt tgcccttctt taccccacgg ctccccttcc cagctttcga
    14701 ccagccagaa ccacagagtt atatgcctgt ccgggaattc atattccagt ttttcaaata
    14761 gcgaaaaatg ttgtggggta atcccgactt cttcctgaag ttcacgcacc accgcctgtt
    14821 ccggcgtttc gcccatttca attttaccgc cgggaaactc cagtttattc gccatgtgcg
    14881 catctgctgc gcgacgcgtt ataaagattt cattgttctc gttgcgaata atacctaccg
    14941 caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc ctgcgccttt
    15001 tacttcaact gttagctttt attgcaggcg accatggcac tgcttgtatt ttttaccaga
    15061 accgcacggg caaggatcgt tacgtcctac tttgcgctca ccggtttgcg ccgccagtgc
    15121 agctgcggct gcagagtcgt catcctgatg gctaagctgc tgcatttgcg ctaaacgctc
    15181 ggcttccata cgacgctgtt gttccagctc ctcaacctct tcaggcatac gtacctggac
    15241 tttgctcagc gtgctgataa cttcatattt taacgactcc agcatcgctg caaacatgga
    15301 gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagccac gcaggtggat
    15361 accctgacgc agatagtcca tcgccgccag gtgctctttc cacagagagt cgagagtttg
    15421 cagcatgacg cctttttcga agtgacgcat catctcagca ccaaccactt cttctttacg
    15481 ctgatacact tcgatggact gcgccagaat tcgctcacgc agcgtctctt catgcagttc
    15541 tggttcttta tccagccact cggcaattgg caaatcgagg tcgaaatcgt tcttcagacg
    15601 ttcctgcagc cccggaatat cccacatttc ttccagtgac tgtggcggaa tgtaggcatc
    15661 aatggtcgct ttgaacacat cttcacgaat gctgttgatg gtttcgctca catcgctgac
    15721 atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag ccacgtcatc
    15781 atattccagc agttgcttac gaatgtcgaa gttacggctt tcaactttac gctgggcgtt
    15841 ggcaatcgct ttagtcaccc acgggtgttc aatggcttcg cctggcttca tacccagttt
    15901 acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat cttccatcga
    15961 caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc gcaactggtt
    16021 atcgatacga cgggattcgt gacgctcggt accgatgata tgcaggccac ctgcttccag
    16081 taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct ctgcggtcgg
    16141 attttccagc gcggcaactt ctgcctgcca gctaccaccg agcacaatat ctgtaccacg
    16201 acccgccata ttggtcgcga tagtcaccgc agccggataa cctgcctgag caacaatcgc
    16261 cgcttcgttg gcgtggaatt tggcgttcag gacgttgtgc ttaataccgg ctttggtcag
    16321 ttcgtttgac accagctccg atttttcgat ggagatagta cccaccagca ccggctggcc
    16381 tttcgcagta cgttctttga tatcttcaat gatcgcctga attttttccg cttcagtcat
    16441 gtagaccagg tccggcagat ctttacgaat cattggacgg ttggtcggaa caacgacggt
    16501 atccagcttg tagatggagc taaattcgaa agcttcggta tcagcagtac cggtcatccc
    16561 cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca gcgtttggtt
    16621 ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac catcggacca
    16681 gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt caccatcttt
    16741 aacgatgtag tcgacgtcgc gggtaaacag cgcatgagcg cgcagcgccg ccgttacgtg
    16801 gtgcatcagc atgatgttgg ccggagagta cagagactcc ccttcatcca tgatgccctc
    16861 tttcaccagc agttcttcaa tcagcaccag accacgttcg gtcaggttca cctggcgaga
    16921 tttttcgtcc accgagaagt ggccttcgcc ctggaaggtt tcggagtctt ctttttcctg
    16981 acggatcagg tgcggaataa ttttattcac gcgtttatac atttccgagc tgtcttctgc
    17041 cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt ccacttcgtc
    17101 caccagcgca tagtgcagtt tacgctgtac acgttcttca gggctgaacg ccatattgtc
    17161 gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag ctgcgtaagc
    17221 ttcgcgcttt gccggtgctg gcatgcccgg caggttgata ccgacagtca ggccaaggaa
    17281 ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt tgacggtaac
    17341 tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg cggtcagggt
    17401 tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca taccgccgag
    17461 taactgaacg tcgaagtgac gcataccaaa gacgcgctta cttgcctcac gtaccacggc
    17521 gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgtg cacgaaactc
    17581 tgcggttttc cctttcagtt cttcgtcgga gagtttttcc atctccggtt ccatggcatt
    17641 gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac taccgaaaac
    17701 tttagttaac aatttgatta gcataataaa atctcaaacg ccccgcgttg cggagttaat
    17761 aaaatgaagt aaaggtttat tgttgttagg tgaggcgttg agggccagca cggatgcctt
    17821 gcgcctggct tatccagacg ggcgtgctga attttgcttg tggggtaaaa tgcgcataat
    17881 caatgcgata acccttttca gacggcgtgc cttcctgggt cagcagcgcg ctgagcgtat
    17941 ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg ggcagtgttt
    18001 gcggtgccat tgcgaaagaa agatgacgga ttaccgtgcg aatggcatgt tgatgccagt
    18061 aatcaacgga atagttcgaa ttcgggcggc gtgtgttcgc ttccagcaag gccaattgac
    18121 caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg ggcgcgtttg
    18181 gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc cgcaaccatc cctaataaga
    18241 gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt ccactcacgt
    18301 tattgccatc ccgatcatat tcaaacgtat ttagcgcaca aatcttatca ttaaagcctg
    18361 cagtccacag cacgaatgaa gataagttgt gcggaaaagt gcttaagaaa acagcgttcg
    18421 caccgttttt tatagctctt ataaaattca aaaaaaaacg actggttcac ctggccggag
    18481 agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt atgccagtac
    18541 agctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg tcacatattc
    18601 ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg ttattcagtg catgaccgga
    18661 tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt caccgatcgc
    18721 atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt cgttcagtac
    18781 gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca aaccacggga
    18841 ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga tctggcgcat
    18901 aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa tagccggatg
    18961 gttaaaatcg atggtgaaat ccagcgaaaa accattgtac ggcttaaatt cagcccactt
    19021 atcgccatct tcgacacgaa cagtctcttt gatgcgaaca aattttttgg cgcagttcaa
    19081 ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc cgtccatgat
    19141 cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaggc ccgcgagagc
    19201 agcattgagg tgctctacgg ttgaaatccg tacatcatgc tcgttgacca gacacgtaca
    19261 gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg gattcaagtc
    19321 ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca gagtgacttt
    19381 cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg tcctttgttt
    19441 gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata cattcggcgg
    19501 gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa tcagcttgct
    19561 tacgcaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc ggcgcattgt
    19621 cattcacgac tttagcaacc ggcttctgct cctgggtcag cggagccatc ccatgctgct
    19681 ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg atttcaggac
    19741 gtttgtccat gccgatacct gtcgcaacaa cggttacgcg cagctcgtca ttcatatccg
    19801 ggtcaagaga agtaccgata accacagtcg cgttgtcgga agcaaatgca cggatggtgt
    19861 tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgccgtgatg ttaaccagca
    19921 cgccgcgcgc gccagacagg tcgatatctt ccagcagcgg agaagagata gccatttcag
    19981 cagcttcttc cgcacggtct tcaccgctcg ccacgccaga acccatcatt gcgtagccca
    20041 tctcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc ggacgagtaa
    20101 tcagttcagc gataccttgc acagcgcctt tcagtacatc gttcgctgcg ccaaacgcat
    20161 ccagcaggga gataccgcgg cccagaactt tcagcagttt gtcgttcggg atagtgatca
    20221 gagagtccac atgcttggac agttcagtga tcccctgctc cgcgaatgcc atacgcttct
    20281 tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt caggataccc aaatcttttg
    20341 ccacttcagc gacgactggt gctgcacctg taccggtacc accacccata cccgcagcaa
    20401 taaagaccat gtctgcacct tccagcgccg cacgcaatgc atcgcgatcc tcatcagccg
    20461 cattgcggcc aacttctgga ttagcgccag cgcccagtcc tttggtgata ccgctaccga
    20521 tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta tttaccgcga
    20581 agaattcaac accttcaatg cgctcgcgca ccatgtgttc aacagcatta ccgccgccgc
    20641 cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt tcaaacatag
    20701 tttctctccg atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct cataaaaatt
    20761 aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact gatgctgtaa
    20821 cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccatag tgaagcaatc
    20881 ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta atgttcagcg
    20941 gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc acaggctgca agaccttcga
    21001 tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta accccttgtt
    21061 ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac caggttgagc agctcggtat
    21121 agcgcggctc gatcacctct gccagtgtct gacgttgcag actccgtggc ggacgaccac
    21181 ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggaacccagc gcacaaccgt
    21241 ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcgtaa gcgatatcac
    21301 tggtcacgac attgccagca taaggaatta ccttagtgtg gcgcaatgcc ccaccggtat
    21361 aaacggcgat atccattgta ccaccaccga tatcgacgac gcagacaccc agttcacgtt
    21421 catcttccgt caataccgaa taacttgatg ccagtccggc aaatatcagt tggtcaactt
    21481 tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcgttg tgacatgtga
    21541 tcaggtgcac ttttgcctgc atccgcacgc ccgaaagtcc taccggattc ttgatccctt
    21601 cctgatagtc aatcgcatac tcttgcggga tcacatgcag cacacgatgc tcatcgcgca
    21661 cacgcaccga tttcgcggta tggacgacgt tttccacatc ttcttgcgtt acttcttctt
    21721 cagaaatagg caccatacca atttcattct ggcagctgat atgcttacca gaaagcgcca
    21781 gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg gcgcgctgta
    21841 cgcacttgac cacggattcg aggtcgttca ccccgccttt atccatacca cgcgacgggc
    21901 agctgcccac gccaatgata ttgaccatac cgtcgggcag aacttcccct actaaagcgg
    21961 caaccttcgc ggtaccaatc tccagtccta ctaccagttt tctgtccgtc gccttgatca
    22021 ttgttgttct gcctgtgcct gattttgttg ctgagtagat tcctctggcg gcaagggcgc
    22081 ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc gtttgccatc
    22141 ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca aacgtttcat
    22201 cgtatcgccc cggccaagat tgagcttaat atcgttattc agcgtcaact gccaggaacg
    22261 ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca tctgccccat
    22321 ttcgcgatag ccctgcaaca cttcattagc gctgccttcc gggccataca gcattggaag
    22381 cacctgcttg ctggtgcgat ctggcggcac gctgaaggtg tttccttccg cgtctaccat
    22441 atgttgatca ttccaccgcg caatcggcac atattcaacc agatgaatct tcaattcatc
    22501 aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt ctatttgcgt
    22561 ctggatgatg ttgacatcct gggtcataaa ggtacccggc tcacccaatg ccaggatcga
    22621 ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca gctttgagag
    22681 cggcaggcgt tgcgcatctt ccatccagcc caacacgacc cagccgctca ccaacactgt
    22741 cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat tgcggcgaga
    22801 agaaacctct tcttcgctgt ttcgcgtgtt cagagcagcc tgcgacatat tagtccgcca
    22861 gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt gccgccatcg
    22921 gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaga taaaactgtc
    22981 catcgctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac gtcgtccatg
    23041 ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct gcggggcaga
    23101 aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt ccggccggtt
    23161 gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac tccggcccac
    23221 ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt aatgcatctt
    23281 gtagagcatt ttctgctact acttttgaca ttcccacact ggaaccttcg cggctcggct
    23341 taacgataac cggcaaaccc agagcagaaa tttctgctaa ctgcttatca ctcaagcctt
    23401 tttcaaactc tgcgcgggtt aacgctaccc acggcgcgac cggtaaaccg gcaccttgcc
    23461 acagaagttt gctgcgtagt ttatccattg aaagcgcaga tgccatcact ccactgccgg
    23521 tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt accatcttca ccgccgcgac
    23581 cgtgtagcgc gataaacact ttctggaagc ccatcgactt cagttgcgtc acgtcgactt
    23641 ctttcgggtc gacaggatac gcgtcaatac cgccttcacg cagcccggct aacaccgctg
    23701 cgccagaatt cagagaaact tcccgctcag cggaggtccc gcccaacagg accgcgattt
    23761 tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc agctaaagaa
    23821 cgggcaattt ttccgatatt accagccccc tgaacgagaa tcaggtcgtt accggttaat
    23881 accggtgcca gcatctcggc tacctgcgca gggtccggca ccagaatggc atcaattttc
    23941 ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat tggcgcttcg
    24001 ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac attggcgaaa
    24061 tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat taccaggttt
    24121 ttatccggcc agcctgcgcg cgccgcttta atggtggcgt caacttccgt cgggtggtgg
    24181 ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcactggctc cagcgggaat
    24241 tcaccgagga aatcaaaacg gcgcccagtc ccctggaagc tctccagcgc acgtaaaata
    24301 gcctcgtcat caattccctc ttccgtagca accgcaaccg cagctgcggc gttcagcgcg
    24361 ttatgacggc ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg gcgcagcagc
    24421 gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc ggcatcttcg
    24481 ctgaagccgt aagtcgtggt ctgacgtccc acacgcggta acaattcgcg gatcaccgga
    24541 tcatcaacac acatcaccgc acgaccgtaa aacggcaggt tgtgcagaaa attaataaaa
    24601 gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc ttcgatattg
    24661 gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc atctgcttcg
    24721 gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt taccagcccg
    24781 ccgttaacga aggttgggtc gagccccgct tctgtgtaga tgctggaaac catcgcggtg
    24841 gttgtcgttt tgccgtgcgt tcctgcaatg gcgatgccat gacgaaaacg cattaactca
    24901 gccagcattt cggcacgacg gatcaccgga atacgcgctt catgagctgc gacaatttcc
    24961 gggttatcgg cagaaatggc gctggaaaca acgaccacgc tggcatcacg tacgttttcc
    25021 gggcgatggt tgaaataaat cgtcgctccc agattcatta actgctgcgt gaccggattt
    25081 ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc ggcaataccg
    25141 cccataccgg caccaccgat accgacaaaa tgtatgtgcc gaacgcgacg catttcgggc
    25201 acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat taacttctta
    25261 aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg ctcacttcat
    25321 ttgccactcg ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct gccatggtta
    25381 ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg ctaagctgtg
    25441 gctgctcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc cagtattgct
    25501 ggcggtcttt atgttgaaac ggcacaaaca acgccggtag tcctgccgcg gcgatttcac
    25561 tcaccgttaa agcaccggag cggcaaacga cgacatccgc ccacgcatac gccgccgcca
    25621 tatcatcaat aaattccgtc actttatgct gcggttgccc cgcttcggca tacgcctgtt
    25681 caacggattg ttgcgaacct ttaccgctct gatgccagat agtgactgaa tcacccagtt
    25741 tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc gccctgggaa ccacccacta
    25801 ccagcacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac ggcagcgcca
    25861 gcacatcggt acgcaccggg ttacccacca cttccgcatt agggaaagcg cctggaaacg
    25921 cctgcatcac tttggtggca atcttcgcca gccatttatt ggttaagccc gcaataccgt
    25981 tttgttcatg aagtacaacc ggaatgccta acgaccacgc ggccagacca cctggacctg
    26041 atacatagcc gcccataccg agtaccacgt caggtttgta cgctttcata atcgcccgcg
    26101 cctgacgcca ggcgttgaag atacgcagcg gcgcagctat cagtgctttt ataccttttc
    26161 cacgcagacc agagatacga atgaaatcaa tttcgatgcc atgttttggc actaagtccg
    26221 cttccatacg gtcggcagtc cccagccagc gaacttgcca accctgagcc attagatggt
    26281 gcgcaaccgc cagtcccggg aatacatgcc caccggttcc gcctgccatc accattaatc
    26341 gctttccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct ccagacgcgt
    26401 ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta agctcgaacc
    26461 accgtaactg atcagcggca atgtcagacc tttggtcggt aacatccccg ccgccgcgcc
    26521 tacgttaacc agcgcctgga agctaaacca gatgccaata gaacaggcga gaaaaccgga
    26581 aaaacggtgg tcaatttcta acgctttgcg gccaatcgac atcgcgcgaa aagcgacgaa
    26641 gaataccatt aaaagtgcca gcaccacacc gacatacccc agttcttcgc cgataatggc
    26701 gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg agttacctaa
    26761 accttgtccc caaagttcgc cgcgaccaaa cgccatcagc gattgcgtta actgatagcc
    26821 gctgccaaag ggatcttccc acgggttcca gaatgcggta acacggcgga tacggtacgg
    26881 ttcggcgagt atcagcaaca caaccgctga aatgcccata ccgataatgg caatgaactg
    26941 ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca acaccaccac
    27001 cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatca cgcccatcgg
    27061 tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca gatagttggc
    27121 gatataacaa aacagcgaca gttttgtcag ctccgcaggc tggatacgca gcaaaccgag
    27181 atcgatccaa cgcgatgccc ctttaaccga gctacccact accagcacga tcatcagcag
    27241 gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca tcggcagacg
    27301 cagcgtaatg atcgccagaa tgaatgccag gatcagatag acgccatcac gcttcgcgaa
    27361 gaagaacgga tcgttggtta agcgttgccc tatgggcatt gacgccgagg tcaccatgat
    27421 aaagccaatc gccgcaaggc cgaaggtcag ccacagtaag gtgcgatcgt acatgatcag
    27481 gctgtcggta tctttttccc gcgagcccat cacccagcct tttagcgccg tggagatcca
    27541 gaccaggata ctgaatcctg gcaggcgcgg cattttcagg cgagggagag ataaacgcat
    27601 caacctaact ccttcgccag acgggcaaac tcattgcctc gttgttcaaa gttcttgaac
    27661 tgatcaaggc tggcacaggc cggggagagc agaaccatat cgcccggctg aacacgcgga
    27721 gccagcaagc gcatcgcctg ttccatagtt tcggtttgtt ctgccacttc cgggcgtagc
    27781 gccgccagct gcgcaccgtc acgaccaaaa cagtacaaac gcacgttgtc gccattcaga
    27841 tagcgcgcca gtggactgaa gtccgccgat ttaccatcgc cgcccagcaa caaatgcagc
    27901 gtgccgtcta cctgcaagcc attcaacgcc gcttccgtac tgccgacgtt agtggctttc
    27961 gaatcgttaa tccagcgtac gccattgtgc tccagcacca cttcaaagcg atgcggcaga
    28021 ccggtgaagg tggttaacgc tttcaggctg ctggcgcgcg gcaaaccagc tgcgtctgcc
    28081 agtgccagtg ctgccagcgc attggtgtag ttatgctgcc cggaaagttt catctctttc
    28141 acgttcagca ctttctcgcc tttcactcgc agccaggttt ctccctgctg atgattcagg
    28201 tgatagtcac ccatgttgac gccaaagctg acgcagcgtt catcagcacc gcgaatcggc
    28261 attgttaagg catcatcagc attgacgaca caaactttcg cattttcgta aatacgcaat
    28321 tttgccgcac ggtattgctg caaaccaaac ggatagcggt ccatatgatc ttcagtcacg
    28381 ttcagaatgg tcgccgccac cgcctgtaag ctggaagtgg tttccagctg gaagctcgac
    28441 agttccagca cgtacagttc gcactcagca tcaagcagca tcaacgctgg caggccaata
    28501 ttgccaccca caccaacgtt aacccccgcc gctttcgcca tttcacccac tagcgtggtg
    28561 accgtgcttt tgccgttaga accggtaatc gccacaatcg gtgcttgtgc ttcgcggcag
    28621 aacagctcga tatcgccaac gatttcgatt ccggcatcag cggcagcgct taaggatgga
    28681 tgcgccagtg caataccggg actggcgaca atcagatctg ccgccatcag ccattcatca
    28741 ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt tatccaggcc aggcggtgtc
    28801 atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc cacgcaggaa
    28861 agcccggtga ggcccaggcc gataatgacg acatttttac cctgataatc agccatgatt
    28921 aacgtacctt cagcgttgcc agaccaatca gaaccagcat cagcgaaata atccagaaac
    28981 gcacaatgac gcgcggttcc ggccagcctt tcagttcata gtggtgatga atcggtgcca
    29041 tgcggaaaat acgttgtccg cgcagtttaa aggagccgac ctgcaggatg acagaaagcg
    29101 tttctaccac gaacacgccc cccataatca ccagcaggaa ttcctgacgt agcagtacgg
    29161 cgataatgcc taacgcacca cctaacgcca gcgaacctac atcgcccata aagacctgcg
    29221 ccggataggt gttaaaccac aggaagccca gtcctgcccc gactatcgcg gtacagacaa
    29281 taaccagttc cccggcgtgt cgcagatacg gtatgtgtaa atagctggca aagttcatgt
    29341 tgccggtcgc ccacgccacc agcgcaaaac caccggcgac aaatacggtc ggcataattg
    29401 ccaggccgtc gagaccatcg gtcaggttta ccgcgttgcc agtacccaca atgacgaagt
    29461 aagccagcag aatgtagaac agccccagct gcggcatcac atctttaaag aatgggacca
    29521 ccagctgcgt tgcgggcgtg tctttgccgg caaggtacag ggcgaaggcg acacccagcg
    29581 caatgaccga catccagaaa tacttccaac gagcgatcaa ccctttggtg tctttacgca
    29641 ccactttgcg ataatcatca acaaagccaa taacaccgta acctaccagc accaccaaca
    29701 cgcaccagac gtacggattg gacgggtaag cccacagcag tacggagatc acaatcgccg
    29761 tcaggatcat aatcccgccc atggtcggcg taccgcgctt gctgaagtgt gattcaggac
    29821 cgtcgttacg caccacctga ccaaaggaaa gtttttgcaa atgagcaatc atacgcgggc
    29881 ccatccacaa tgagatgaac agcgcggtca gcaggctgac gatggcgcga aacgtcagat
    29941 aggaaaagac gttaaagccg gaataatatt tgaccaaatg ttcggccagc caaactaaca
    30001 tgtcccattc tcctgtaaag cgcgtactac ctcttccatg gcggcactac gtgaaccctt
    30061 aactaaaatc gtaattacct gttgctcagc aatcagtgat ttaagacgcg taattaacgc
    30121 agttttatca gcaaaatgtt cgccaacgcc gctggcggtg ctgatagcat ggctttgttt
    30181 acccacgctt aacacgcggt caataccagc agcttttgcc gcctcgccca cctgtacatg
    30241 gcaggcttcg ctttcagcgc ccagttccgc catatcgccc accaccagca cgcggtagcc
    30301 cggcatttca gccagtacct gaacagcggc ggtcattgaa ccgacattgg cgttgtagga
    30361 gtcgtcgagc agcaactggt tttctgccag ttggatgggg aacagacggc ctggaacagc
    30421 tttcagattt gccagccccg ctttgatagc atcaagcgtt gcgcccacgg acatggagag
    30481 cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca gcagaacatc
    30541 gacgctaccg gttggggttt gtagggtaaa ttccgtaccg tgcgaggtca catggatatt
    30601 ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa cgccacactt tgcgtgagcc
    30661 aattacgctc tgccagttca gccagtcgtt gttgtcggcg ttcataatgg cgataccgtt
    30721 ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa gcgagccaaa
    30781 accttccaga tgcgccgctg ccaggttgtt gaccagcgca gcttccgggc gagtcagact
    30841 cacagtccag gctatttcgc cctgatggtt cgcgccaagt tcaataactg cgtaatcgta
    30901 ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg tcgttgttga gattgcctgc
    30961 cgtataaagc gtgttgccgc actggcttaa aatcgccgcc gtcatctctt taacggaggt
    31021 tttgccggag gaccccgtca gagcaaccac gcgcgccgga acttgctggc gaacccatgc
    31081 agccagttca ccaaacgcca gacgcgtatc cttgacgatt aactgcggca ggtcgatatc
    31141 cagcggacgg ctgaccagta gtgcgcccgc gccgccagct ttcgcctggt cggcaaaatc
    31201 gtgggcatca aaacgttcgc ctttcagggc aacaaacagg cagcccggcg tcagttttcg
    31261 ggtatcagtg gttacagcat caagggtgat atctgcacct tgcagttcac cgttgagaat
    31321 gtcggtaagt tggctaaggg ttacgctaat catgcaatca cccccagcag acgcgccacc
    31381 gtgacgcgat cggagtagtc cagacgctga ttgccaacaa tctggtaatc ttcatggcct
    31441 ttgcccgcga ccagcaccac atcgttctct ttagcctgca taacggcgca agtcaccgct
    31501 tcagcacggc cttccatcac tttggcatgt ccggcatcta acattcccgc cagaatatcg
    31561 ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac cgccacgtca
    31621 gcaaactctt cggcaattgc gcccatcagt ggacgcttac ctttatcgcg atcgccacca
    31681 cagccaaaga cacaccacag cttgcccgca cagtgcagac gcgccgcctg taaggctttt
    31741 tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg tcggtttgcc tggcgcagtg
    31801 aacacttcca tacgtccgca aaccggttgc agacgcgcgg cggttttcaa cagatcagcc
    31861 aatggatagc cgagtgccaa cagtgtcgcc agcgcgagca gcaggttgct gacgttaaaa
    31921 gcgcccatca gatggctttc aatttcgcca tcgccccaac ttgagctaaa gcgaatcgtc
    31981 gcaccgctgt cgtgatagtt cacgtcgatc acttttaacc agcgtccgtg acagttcgga
    32041 ttaatatgat cttccattga taccgcaacc gcgtccggca gctttgccag ccaacggcgg
    32101 cccacttcat cgtcggcgtt aataatcacc tgaccgcaat gatgttcaga ataaagcagc
    32161 catttcgcag cttcgtagtg ttccatatca ccatgataat caaggtgatc gcggcttaag
    32221 ttggtaaaga ccgacgccgc aaatttcaat gccgccacac ggtgctgtac cagcccgtgg
    32281 gaggaaactt ccattgcgca aaacgtcgcg ccctgctcca ccagccccgc cagctcatgc
    32341 tgaacatcga ctgccgaacc ggtggtattt tctgtcggga tcactttccc cagcagaccg
    32401 ttaccaacgg tgcccattac cgcgctggtt tcgccaagca gttggctcca ctgcgccagc
    32461 agctgggtag tcgtggtttt gccgttggtg cccgttacgc ctacgagacg taaattgtca
    32521 gagggttcat ggtaaaagcg gcccgccagt gcagataaac gctcgttgag ctggctgaga
    32581 tagatgaccg gtacgccgtg catttcacgg atttcaccgt cggtcgcctc atctttcgcc
    32641 tctgcaataa tggcagccac accttgcgct atcgcctgcg ggatatatcg acgcccgtcc
    32701 gcctgatgac ctactacagc tacaaagaga tcgcccgccg cagccacacg gctgtcgagt
    32761 gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag aaggtcgcgc
    32821 aaattacgat ctgccacctg tcccctcgcc ttgattaatc acaaattcat ttttatcgcc
    32881 cgttgtcagc gcatccggct cgatgttcat ggtacgcaat acgccgccca tgatggcacc
    32941 aaagaccggc gcggaaacgg cgccgccgta gtatttaccc gcctgcggat cgttgataac
    33001 aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat aagcaatata
    33061 tttattgatg tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt taatggcgat
    33121 acgatagcct ttaatcgccg ccttcacgcc gccgccgcct ggtagcgcca cgctttccat
    33181 catatgcacc acagtgcgga caatggattc cgggaagaca cgttcaccgg gaaccggggg
    33241 gtcaactttg gtaatcgaca gtgggcgata aatgccgtaa ctgccgatag ttgcgtagac
    33301 tcgcgctaac tgtaacggtg ttaccattag cccgtagccg aaagagaagg tggccctctc
    33361 tatgtcagac caccgttgtt tttgaggata taagccactg cgttctccga ccaaccccaa
    33421 attggtcgct tttcccagtc caaaacgtga gtaagtatct actaacgctg aggacggcat
    33481 cgctaacgcc agcttggaaa caccgacgtt actcgacttc tgtaataccc cggtcagggt
    33541 taattcgctg tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat aaggaatggt
    33601 attgagtacc gagttttccc gcaccacgcc acgttgcaac gcggtcatta ccaccatcgg
    33661 tttaaccgtt gagcccggtt caaacacgtc ggtgatggta cggttacgca tcgcctcttt
    33721 cggcgtgccg ctcagattgt tagggttgta tgacgggctg ttagccatcg ccagcacttc
    33781 accggtgttg acatccacca gcacggcgct accagattca gccttgttaa aggccaccgc
    33841 gttgttcagt tcgcgataaa ccagcgcctg caggcgttca tcaatactca gcgccaggtt
    33901 gtgcgctgcc tggctgtcag tagaagaaat atcttcaatt acgcgaccat agcggtcttt
    33961 acgcacaatg cgctcacccg gctgcccggt aagccattta tcgaaactct tctcaacgcc
    34021 ctcaatccct tgactatcga cgttagtaaa gccaatgagg tgagcagtca cttcgccgga
    34081 tggataataa cggcgagatt cttcacgcag atgaatcccc ggcagtttca gttttttgat
    34141 gtagtccgcc atgtcagggt ttacctgacg cgccagataa ataaagcgcc ctttcgggtt
    34201 ggcgttaatg cgggctgaaa gctgatccag cggaatattg agcgcgttag ccagcgcctt
    34261 ccagcggtca ccgacgctga taccgccagc gtcatgcact tctttcgggt cagcccaaat
    34321 cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa tcatgccgcg
    34381 ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg ccctctttca ccagcatatc
    34441 cggggagata acttgtaacc acgctacgcg tccgagcaga aaagccagcg ccaggagaat
    34501 acagccgcat aacaacgcaa aacgccaact gataaagttg gcatgttctt cctgacgttt
    34561 tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt tttgcactac
    34621 gatattttct tgtgacggat caacatgctg catttgcagt ttttccgtgg cgatcctttc
    34681 cacccggcta tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc attcaatgtc
    34741 taaagcatct cgctccagca ccagttgttc gcgctgagcg gtcagtaaac gggtatggtg
    34801 cgccgtggtt accacggtca ccgccgtcaa aataatgcaa atgaacaggc agagtggcag
    34861 cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc gctcgtggct
    34921 tcccatcgat cctttaactt tgcttagagc ttctgtcact ctgctgatca tgcgttcgtc
    34981 ctctctgcaa tacgcagaac tgaactacgg gcacgaggat tctcagccac ctcttcttcg
    35041 cccggcatta acttgcctaa tgctcgcagc tgacggccac ccagtttttt gagctgctct
    35101 tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg cataaaacgt
    35161 ttcacaatac ggtcttccag cgagtggaag ctgatgatcg aaagccgccc acccggggcc
    35221 agcacgttga gcgagctttt tagcgcctgc tctatctcct ccagttcact gtttacccaa
    35281 atgcgcaccg cctggaaggt acgggtcgcg ggatgtttga atttgtcttt caccggcgtt
    35341 gcggcagcca cgacttccgc cagttctttg gtgcgggtca tcggctgttc gcggttacgc
    35401 tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt caccataggt tttcaatacc
    35461 caggcgatat cggcttcttc tgcggtttgc agccattcag cggctgactg cccacgggtt
    35521 gggtccatac gcatgtccag cggaccatcg cgcataaagg aaaagccacg ttcagcatca
    35581 tcaagttgcg gtgaagagac gccaagatcg aggagaatgc cgtcgatctt gccgataaga
    35641 tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc cgtggatgat ggagaagcgc
    35701 ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat cgccagcaaa
    35761 cgcccctctt cgccaagctg cgagaggatc agacgtgagt gaccaccgcg accaaaagtc
    35821 ccatcaatgt agatgccatc aggacggata ttgaggccat taacggcttc atccagcagc
    35881 accgtagtat gtttatagtt ttccatcatt ttatagagac aagtcctgca gtcgctccga
    35941 taagtctcca gtagccaact gctctgcgtc gatatcttcc ttgacctgtt gatgccaggt
    36001 tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc agcatcactt cttttgtcag
    36061 cccggcatgt tgccgcagta ctggcgcgat taacaatcga cctgcgccat ccatctgaca
    36121 ttcgctggca tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt tcatgctcga
    36181 cagacgcgat aatttttgct cgataatttc ccattcaggc agggggtaaa gcagcaggca
    36241 cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa gcagctgttc
    36301 ccggtaacgg gtaggcactg ataagcgccc tttgctgtcg agattgacta acgttgctcc
    36361 ccggaacatg ccagcctcac cccttattcc cactttgccc cacaatttcc cactgaaagg
    36421 agtttacgga gcggaggaaa agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa
    36481 cccgatgttt acggctaata tcagcgttgt ttattttacg tccaacgtgc gcagcaaatt
    36541 aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt aaaatgactc
    36601 actattttcc acagcaaata taaagtgtca gtttgcgacg cgagcggcat tttaggacat
    36661 atcttccccg gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca gcgggtttgc
    36721 gcaaaagcaa agaaaagtgc t
//
LOCUS       DAJSKJ010000017        36537 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000017 DAJSKJ010000000
VERSION     DAJSKJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.017"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..107
                     /locus_tag="ON345_000768"
     CDS             <1..107
                     /locus_tag="ON345_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR3070877.1"
                     /translation="QTDNDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            458..1375
                     /gene="gcvA"
                     /locus_tag="ON345_000769"
     CDS             458..1375
                     /gene="gcvA"
                     /locus_tag="ON345_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR3070878.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            1394..1789
                     /locus_tag="ON345_000770"
     CDS             1394..1789
                     /locus_tag="ON345_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR3070879.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            1782..2882
                     /gene="rlmM"
                     /locus_tag="ON345_000771"
     CDS             1782..2882
                     /gene="rlmM"
                     /locus_tag="ON345_000771"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR3070880.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(2926..3657)
                     /gene="fucR"
                     /locus_tag="ON345_000772"
     CDS             complement(2926..3657)
                     /gene="fucR"
                     /locus_tag="ON345_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR3070881.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(3715..4137)
                     /gene="fucU"
                     /locus_tag="ON345_000773"
     CDS             complement(3715..4137)
                     /gene="fucU"
                     /locus_tag="ON345_000773"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR3070882.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(4139..5557)
                     /gene="fucK"
                     /locus_tag="ON345_000774"
     CDS             complement(4139..5557)
                     /gene="fucK"
                     /locus_tag="ON345_000774"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR3070883.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(5666..7441)
                     /gene="fucI"
                     /locus_tag="ON345_000775"
     CDS             complement(5666..7441)
                     /gene="fucI"
                     /locus_tag="ON345_000775"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR3070884.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQS
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(7474..8790)
                     /gene="fucP"
                     /locus_tag="ON345_000776"
     CDS             complement(7474..8790)
                     /gene="fucP"
                     /locus_tag="ON345_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR3070885.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIKSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            9337..9984
                     /gene="fucA"
                     /locus_tag="ON345_000777"
     CDS             9337..9984
                     /gene="fucA"
                     /locus_tag="ON345_000777"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR3070886.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            10012..11160
                     /gene="fucO"
                     /locus_tag="ON345_000778"
     CDS             10012..11160
                     /gene="fucO"
                     /locus_tag="ON345_000778"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR3070887.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVLAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(11215..11970)
                     /gene="xni"
                     /locus_tag="ON345_000779"
     CDS             complement(11215..11970)
                     /gene="xni"
                     /locus_tag="ON345_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR3070888.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVSGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(12082..13449)
                     /gene="sdaB"
                     /locus_tag="ON345_000780"
     CDS             complement(12082..13449)
                     /gene="sdaB"
                     /locus_tag="ON345_000780"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR3070889.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(13507..14796)
                     /gene="sdaC"
                     /locus_tag="ON345_000781"
     CDS             complement(13507..14796)
                     /gene="sdaC"
                     /locus_tag="ON345_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR3070890.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(15353..16717)
                     /gene="ppnN"
                     /locus_tag="ON345_000782"
                     /gene_synonym="ygdH"
     CDS             complement(15353..16717)
                     /gene="ppnN"
                     /locus_tag="ON345_000782"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR3070891.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLDENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(16829..17677)
                     /gene="queF"
                     /locus_tag="ON345_000783"
     CDS             complement(16829..17677)
                     /gene="queF"
                     /locus_tag="ON345_000783"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR3070892.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSINLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            17745..18290
                     /gene="syd"
                     /locus_tag="ON345_000784"
     CDS             17745..18290
                     /gene="syd"
                     /locus_tag="ON345_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR3070893.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18912..19241
                     /locus_tag="ON345_000785"
     CDS             18912..19241
                     /locus_tag="ON345_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR3070894.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            19241..20023
                     /gene="truC"
                     /locus_tag="ON345_000786"
     CDS             19241..20023
                     /gene="truC"
                     /locus_tag="ON345_000786"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR3070895.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            20041..20490
                     /locus_tag="ON345_000787"
     CDS             20041..20490
                     /locus_tag="ON345_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR3070896.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            20925..22277
                     /gene="gudP"
                     /locus_tag="ON345_000788"
     CDS             20925..22277
                     /gene="gudP"
                     /locus_tag="ON345_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR3070897.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            22279..23619
                     /locus_tag="ON345_000789"
     CDS             22279..23619
                     /locus_tag="ON345_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR3070898.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            23640..24980
                     /gene="gudD"
                     /locus_tag="ON345_000790"
     CDS             23640..24980
                     /gene="gudD"
                     /locus_tag="ON345_000790"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR3070899.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(25212..27968)
                     /gene="barA"
                     /locus_tag="ON345_000791"
     CDS             complement(25212..27968)
                     /gene="barA"
                     /locus_tag="ON345_000791"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR3070900.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFIVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            28025..29326
                     /gene="rlmD"
                     /locus_tag="ON345_000792"
     CDS             28025..29326
                     /gene="rlmD"
                     /locus_tag="ON345_000792"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3070901.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            29374..31608
                     /locus_tag="ON345_000793"
     CDS             29374..31608
                     /locus_tag="ON345_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR3070902.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            31686..31934
                     /gene="mazE"
                     /locus_tag="ON345_000794"
     CDS             31686..31934
                     /gene="mazE"
                     /locus_tag="ON345_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR3070903.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            31934..32269
                     /gene="mazF"
                     /locus_tag="ON345_000795"
     CDS             31934..32269
                     /gene="mazF"
                     /locus_tag="ON345_000795"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR3070904.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVVPEELQLI
                     KAKINVLIG"
     gene            32340..33131
                     /gene="mazG"
                     /locus_tag="ON345_000796"
     CDS             32340..33131
                     /gene="mazG"
                     /locus_tag="ON345_000796"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR3070905.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            33359..34996
                     /gene="pyrG"
                     /locus_tag="ON345_000797"
     CDS             33359..34996
                     /gene="pyrG"
                     /locus_tag="ON345_000797"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR3070906.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            35084..36382
                     /gene="eno"
                     /locus_tag="ON345_000798"
     CDS             35084..36382
                     /gene="eno"
                     /locus_tag="ON345_000798"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR3070907.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(36366..36506)
                     /locus_tag="ON345_000799"
     CDS             complement(36366..36506)
                     /locus_tag="ON345_000799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070908.1"
                     /translation="MAADLAAVAVLLEVAAHPAAGNSTIKNASPEAGIFKSDKVSLMP
                     GL"
BASE COUNT         9094 a         8824 c         9707 g         8912 t
ORIGIN      
        1 cacagactga taacgatacc ggtatgattt cgtataaaga cgctaatggc aacaaacagc
       61 agatcaaccg tactgacgtg aaagagatgg tcgaactgga tcagtaattc gcgatcgcaa
      121 ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat cactatggac agacagggta
      181 aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg tctgaatcgc agaccaattg
      241 caaacacaac aacacaacat cacaaccgta agccaaaagt tcaccagaac acgcattccg
      301 ataaaacttt tcgttccggc tcaggaagtg ccgccactat aggtatttgc tggtagaagc
      361 tcaacggaca atttataatg gctcagatta aaaaaactaa taggttacac agtgtgatct
      421 aattgttaaa ttcatttaac atcaaagttt aaaagccatg tctaaacgat taccaccgct
      481 aaatgcctta cgagtttttg atgccgcagc acgccattta agtttcactc gcgcagcaga
      541 agagcttttt gtgacccagg ccgcagtaag tcatcaaatc aagtctcttg aggatttttt
      601 ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag
      661 ctatttcctc gatatcaaag agatattttc gcaattaacc gaagcgacgc gtaaactcca
      721 ggcccgtagc gccaaggggg cgttgacggt cagtttactc cccagtttcg ccattcattg
      781 gttggttccg cgactttcca gctttaattc agcttatccg ggaattgacg ttcgaatcca
      841 ggcggttgat cgtcaggaag ataagctggc ggatgatgtt gatgtggcga tattttatgg
      901 tcggggcaac tggccggggc tacgggtgga aaaactgtac gccgaatatt tattgccggt
      961 gtgttcgccg ctactgctga ctggcgaaaa acccttgaag acaccggaag atctggctaa
     1021 acatacgtta ttacatgatg cttcgcgccg tgactggcag acatataccc gacagttggg
     1081 gttaaatcat atcaacgttc agcaagggcc aatttttagc catagcgcca tggtgctgca
     1141 agcggctatc cacgggcagg gagtggcgct ggcaaataac gtgatggcgc aatctgaaat
     1201 cgaggccgga cgtcttgttt gcccgtttaa tgatgttctg gtcagtaaaa acgcttttta
     1261 tctggtttgt catgacagcc aggcagaact gggtaaaata gccgcctttc gccaatggat
     1321 cctggcgaaa gccgctgctg aacaagaaaa attccgcttt cgttatgaac aataatttac
     1381 gtagggtacg accatgacca gccgttttat gctgattttc gccgccatta gcggcttcat
     1441 ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt aaaaccatgg gggccgttga
     1501 gatgggctgg atccagaccg gcctcgaata ccaggcgttt catacgctgg cgatcttagg
     1561 tctggcggtg gcaatgcagc gtcgcatcag tatctggttt tactggagta gcgttttcct
     1621 cgcgttaggc acggtgttgt tcagcggcag cctttattgc ctggcgctgt cccatctgcg
     1681 tttgtgggcg tttgtcactc cggttggcgg cgtgagcttc ctcgcgggct gggcgttaat
     1741 gttagtcggt gctatccgtt taaagcgcaa gggcgtaagt catgaataag gttgtattgc
     1801 tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat taccgataaa gccggccagc
     1861 gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt
     1921 atcaacctga tgatggcgat aagttaatcc gtgagctgcc gttcagttca ttaatttttg
     1981 cccgccagtg gtttgtggtg ggggaactcc tgcagcattt gccgccagaa gatcgtatta
     2041 cccccattgt cggcatgtta cagggcgtag tagagaaggg cggtgaactg cgtgttgaag
     2101 ttgccgatac caacgaaagc aaagagttac tgaaattctg ccgtaaattt actgttccgc
     2161 tacgcgctgc cttgcgcgat gccggggtgc tggcgaacta tgaaacgccg aagcgtccgg
     2221 ttgtgcatgt attctttatt gcaccaggct gctgctatac cggttactca tacagcaaca
     2281 ataattcgcc gttctatatg ggcattccgc gcctgaaatt tccggcagat gcgccgagtc
     2341 gttccacgct caaactggaa gaggcatttc atgtgtttat tcctgcggat gagtgggatg
     2401 aacgcctggc gaacgggatg tgggcggtgg atttaggcgc ttgccctggc ggctggacct
     2461 accaactggt gaagcgcaac atgtgggttt attccgtcga caacggcccg atggcgcaaa
     2521 gtctgatgga taccggacag gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga
     2581 cgcgcagcaa tatctcctgg atggtatgcg atatggttga aaaaccggcg aaagttgcgg
     2641 cattgatggc gcagtggctg gttaatggct ggtgccgtga aaccattttc aacctcaaac
     2701 tgccgatgaa aaaacgctac gaagaagtgt cacacaatct ggcgtatatt caggcacagc
     2761 ttgatgaaca tggcataaat gctcagattc aggcacggca gttgtatcac gatcgcgaag
     2821 aagtgacggt gcacgtccgc cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat
     2881 aacaggcaag gccggggcgt cgtttccggc ctgctaccct ttccgtcagg ctgttaccaa
     2941 agaagttgca acctggcgct catcagaaat aatgtgcgtt acctcatcca gatgcccgat
     3001 gcgggcttcc cctgaacgat taaatttact tttatcaatc aataacaacg attgcgcggc
     3061 acgttttaat agcatcgatt tgtaatcagc gttgatggca ttggagtccc acagtgcgcc
     3121 gctgctatcg atcccttcac aagaaaaaat aaacagatcg atttccagcg atttaagttg
     3181 ggaaatcagc gagggattga cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact
     3241 gatcagttga atgcgttcgc gtttaccgag ttcatggcaa atcggatggc tattggtgaa
     3301 gacctgaatg ttgatgtcag gcaactggcg tgccagatac cagcaagttg aactggcgtc
     3361 taaggctatc accatccctt cttcaatcca ggcgagcgcc tcgcgcgcga tatctgcttt
     3421 atgcgcataa tggcttttca gcctgatgtg aaagggatcg ccactgtctt gattttgacg
     3481 gtggatatat ttagcgcgtc catgattgcg cagaatttta ccctgcgtct gtaattcatt
     3541 gagatcgcga cgaatggttt ctttactgac ctttagctgt tcagagagag cttccgtggt
     3601 caggctggta tggttcagca gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt
     3661 tttcacccca tcattcgata accgcccgcg tcatgcgggc gtaccggtat gagattacgg
     3721 tgttacccct ttttttaaaa gaatattccc gtacttcgct cgttcgcctg tgataacgat
     3781 cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg
     3841 acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt tctacttcag gatcgagagt
     3901 gtcaccttct accgccgcca tcatcaccag cggcggtgca taactgtcca gttcaaataa
     3961 cgggataatc gcctggagca agtcgctcac caacaggcca tcagcgcgga tcacctgcgg
     4021 tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata atttcatctc catgtcccat
     4081 ctctgccagc actttcaata gttcgggaga aattaacggc gaaattgttt tcagcatttc
     4141 acacttcctc tataaattca ggttcagttt gcgggtagaa ataacggtac tgataatgaa
     4201 tctgtgcgcg ggcttcttcc gggctgttaa attcccctac gccataccaa ccgaacagcg
     4261 cagctcctgc gacggtcgtt tcggcgtcgt cgagaacttt taccggaata tcaagcatat
     4321 tggctttaat ctgattccac aatgtgttgc gacttcctcc accgactaac aataattcag
     4381 aggccttaaa gtgcccgatt ttttccagca tctgtagatt gcgctgtaat tgcgcagtta
     4441 acccttccag cgccgcgcga tagaaatgcc cccgcgtggt attaagcgtc actccttgcc
     4501 agccagcgtt ctgacacgac aataaatcac actgcatttt tacgccatcc gcgccaggcg
     4561 cgatcagacg agcttcttca atcaacattt gccagggtgt ttcagccgtc cagaacagtt
     4621 ttctcaccca ttccagcacg ccggatgcca gccattgcat acctggatta tacaaccctg
     4681 cctggctatc cagttcgcag gtggaaccgg cgtactgact taacagcgaa gtatcaacct
     4741 gggcgctacg gaccattaaa atttcccatg taccggaaga gagcacgggt tcattttggt
     4801 cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc tgcggaaatc accggtatgc
     4861 caacgggtaa gccgagcatt gctgcggcgc tgttctgtag cgtaccaatc tgttcacccg
     4921 cttccaccag acgagggaag agtcggcgtg gaataccggt ggcttgtaaa atttgcggac
     4981 tgaaatcgcg ttgctggata tccagcatct ggctggttcc ggccatcgtg atatcagtag
     5041 tgaattctcc ggttaaacgg tggttaatca gcgacgaaat aaagagccag gcgtgcgcgc
     5101 gttccagcag ttgtggatga ttttctttca accacaccaa cttatataac gtattgaaac
     5161 taaaggctcc gacgccagaa atagcctgca accgctgtgc ggagatcaac tgttcaatat
     5221 tgtccataac cgctgctgtt cgcggacatt tccagctaat gatcggatag agcagattgc
     5281 cttgcttatc taccagagcg ccatccacac caaaggtggt gacggcgata ccgcggatgt
     5341 ggcattcagt cagttcacta ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg
     5401 cgtctaaaga ccactggtgc caggtgttgt tttccatcgc gatatcgctg gcattaggcg
     5461 ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc gatggccctg acattggtcg
     5521 cgccacagtc gagtaccagg ataacttctt gtttcataat cgctcctgca atatagccgg
     5581 ataacattgc ttatccggct aaccactctt gcttgttcag gcccggtagc cggagcgacc
     5641 gggcatcaca tcagggagta atgtattaac gcttgtacaa cggaccgtag ttctggcaag
     5701 cgcggtaatc ctggccttca atatccatgc cgtgcgcagc ccaggcagaa ggacgataca
     5761 ctttggtctc ttcaacgttg tgcatacata ccgggatacg cagcatggag gcgagagtga
     5821 taaagtctgc gccaacgtgg ccgatggtca gaaccccatg gttagcgccc cagttcgcca
     5881 ttaccgagta cacatccgta aacggccctt taccggtgag gcgcggtgca aaccaggtgg
     5941 ttggccaggt tgagttggtg cgtttgttga ggatgtcatg cacatccttc ggcaattcca
     6001 cgctccagcc ttccgcgatt tgcagtaccg gtcccaggcc tttgatgatg ttgacacgag
     6061 tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga agagtaaccg ccgccacgga
     6121 agtattcgtg gatcgccggg caccattcgg tagcggcgag gcaagcgtca gcctcttgct
     6181 gagagatttc ccagtgcggc ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg
     6241 aaccgtccag cgcagcagaa cctgagttga tcaaatggat gatgccgtgt tctgccagtc
     6301 catcgagttt atgccccgtt acacgctcaa ttgcttctgg tgaccagtag gtacgcacat
     6361 cggcaaatac ctgagcggtg ccggtgagct ggtgacccat tagcattgcc acgccgttaa
     6421 gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac gccattccag tcaaatgaac
     6481 tgttgaggat cgcttcggcg gtgtcaccat tgggatattg atcggtccag tgacgttgcc
     6541 cctggaagcc cgcagcgatg gcgttgtagc caagtgattc ttccacgcga ccaatatcgg
     6601 ccagtttgct gttgctttgc atcatgtcgc ggatacacat cgccatcagt aaactttcgc
     6661 gcagaactgc gcggctttgc tcggcattgc gttgatactg tttgttattt tcatcttcgc
     6721 catagcggaa gtttttatca gcccaggtca gtgccatttc cagttcggct tcgtcgtaaa
     6781 tcttctgatc gatacggcga cgcagttcgg tcatatccac cgcctggact ttcattccca
     6841 gccaggattc aaagaagttg tgatcaacaa tagaaccggc gatacccatc gaaacgccgc
     6901 ccagcgacag atagctttta cctttcatgc tggcgacggc caaaccggcg cgggcaaagc
     6961 gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat
     7021 gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg agctgccaga gccgctgcca
     7081 ggtaaacagc gccgggacgt tcagtgccgt taaagcccca aatggctttc gggcgggttg
     7141 gatccatgtc gatggtttca ctgccatagc accagcaagg cgttaccgta atggtgaggc
     7201 ctacattctg actgctgaat ttttcttcgc aagcagcggc ttcagccata cccgcgatac
     7261 aggtatcgga aatgacacac tcgacggcag ctccgcaggc atggcgcagt ttctcggtca
     7321 gcagtgcggc cgtagctttc gccatattca ttgtttgttc ttcaagcgac tcacgaacac
     7381 ccatgcgacg accgtcaata accgggcgga taccaatttt cggtaagctg atttttttca
     7441 ttacagattc ctcactttat tcggaaaata tgttcagtta gttgccgttt gagaacggaa
     7501 acgggcaaag ataaagatga ccgcgaagca gagtgcgggg atcagttcag cagtggggat
     7561 gttgcccgcc gcgtcactga caaaacccat gaccggagtg acaataccgc cgccaatgat
     7621 ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg ccgagattct taatgcccag
     7681 cgagaagatt gttgggtact gaatcgacat aaaggcgctg cataaagtca gggctattaa
     7741 gcccacatga ccgccagcga aggctgagat caggcacagt gccatagcga ttaatgcgta
     7801 ggcggccagg actttgtgtg gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc
     7861 aataaagaag cacaccatgg ttccggttaa atagttagcg gcaaagcctg cagtcatacc
     7921 tggaatttct tctacagcgt agcgaatcaa atagctccag caggccgttt gtgcgccgac
     7981 atagcagaat tgcgctaata ccgcccagcg ccagtgacga atacgcgcca gacgagaaag
     8041 cgatgcggag aacgatcctt gtttggcgtc actgtgatta tcactctgca atgccgggaa
     8101 tttcgtcagc atgatcagca gggcgaccag taacacgata gccacgatga tcatataagg
     8161 tgtctgtacc gataatacca ggctgtgttt atacgcactc aattgctctg gagacatttt
     8221 atcgagaacg tcttgcgatt gatgtggcac gttagacaaa ataagtcttt gcccaaagac
     8281 aaccgcgata attgcgccaa acgagttaaa tgtttgcgca agatttaagc ggaagtgacc
     8341 actgctttcc ggccctaata ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa
     8401 tccggctgca ataataaata ggccaactaa aaataaggtg tagttcatta tttctgcagc
     8461 gggccagaat aatgcagcgc ccagggcata taaaaataac ccagtaataa ttcctgcttt
     8521 ataactgagt tttttcatca atatcccagc aggaattggg ataatgaaat aaccaaagta
     8581 aaaggccgat ttgatcaggc cagcctggaa atttgtcagc gtaaaagcct gctggaattg
     8641 aggtaataaa atgtcgttaa ggttattggc taccgcccaa agaaaaaaca gtgagcacag
     8701 cagcgcgaat ggaataatgt aacttctgct ttgccctgca tctttatcta ccgcacggta
     8761 actctgcgtt tgtattgatg tgtttcccat agcatcctct taggttcaga agcttaagca
     8821 cgtaatcggt tatgttttca aaagtgcaaa accagcgagt tcgggcttca tatcggtgaa
     8881 tatttgcctg tctgaaatga actcatggat tcattttcaa ataaaaaacg ctctgtaata
     8941 tgacggcggt cacacttatt agctggaagg tgactttatg tgactaccat cactttaata
     9001 atcgaaattt catgcccgtt tcagaaaata aatcataaaa aacacaaacc agatgaccgt
     9061 attaattgtt tgtgccgaaa acgggcattt ctggcgaggg gaaatggaaa aatgaccgtt
     9121 ttgataaata tttttaagag cggtcatttt taacggggtt ataaaaagag aacttgcagt
     9181 tatttactgt gattagttga accaggtcac aaaatataag cttgtgaatt tctttcttgt
     9241 taatcaggaa gaagatggtg ggtaattaaa cggctaattc aatagtgtga aaggaacaac
     9301 attattgccc tgttttgaat cagagagagg tagctaatgg aacgaaataa acttgctcgt
     9361 cagattattg acacttgcct ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg
     9421 aacgtcagtg tacgttatca ggatgggatg ctgattacgc ctacaggcat tccatatgaa
     9481 aaactgacgg agtcgcatat tgtctttatt gatggcaacg gtaaacatga ggaaggaaag
     9541 ctcccctcaa gcgaatggcg tttccatatg gcggcctatc aaagcagacc ggatgccaac
     9601 gcggttgttc acaatcatgc cgttcattgc acagcagttt ccattcttaa ccgaccgatc
     9661 cccgctattc actacatgat tgcggcggca ggtggtaatt ctattccttg cgcgccttat
     9721 gcgacctttg gaacacgcga actttctgaa catgttgcgc tggctctcaa aaatcgtaag
     9781 gcaactttgt tacaacatca tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta
     9841 tggctggcgc atgaagttga agtgctggcg caactttacc tgacgaccct ggcgattacg
     9901 gacccagtgc cagtgctgag cgatgaagag attgccgtag tgctggagaa attcaaaacc
     9961 tatgggttac gaattgaaga gtaatttcgt aaagcaacaa ggagaaggat gatggctaac
    10021 agaatgattc tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc tttaaccgat
    10081 gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa
    10141 tgcggcgtgt tggcgaaagt gaccgataag atggatgctg cagggctggc atgggcgatt
    10201 tacgacggcg tagtgcccaa cccaacaatt actgtcgtca aagaagggct cggtgtattc
    10261 cagaatagcg gcgcggatta cctgatcgct attggtggtg gttctccaca ggatacttgt
    10321 aaagcgattg gcattatcag caacaacccg gagtttgccg atgtgcgtag cctggaaggg
    10381 ctttccccga ccaataaacc cagtgtaccg attctggcaa ttcctaccac agcaggtact
    10441 gcggcagaag tgaccattaa ctacgtgatc actgacgaag agaaacggcg caagtttgtt
    10501 tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg acgctgacat gatggatggt
    10561 atgcctccag cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg
    10621 tatattaccc gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc gattgaaatc
    10681 attgctgggg cgctgcgagg atcggttgct ggtgataagg atgccggaga agaaatggcg
    10741 ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt
    10801 atggcgcatc cactgggcgc gttttataac actccacacg gtgttgcgaa cgccatcctg
    10861 ttaccgcatg tcatgcgtta taacgctgac tttaccggtg agaagtaccg cgatatcgcg
    10921 cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa tgccgctgtt
    10981 gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc cacatttgcg tgatgttggt
    11041 gtacgcaagg aagacattcc ggcactggcg caggcggcac tggatgatgt ttgtaccggt
    11101 ggcaacccgc gtgaagcaac gcttgaggat attgtagagc tttaccatac cgcctggtaa
    11161 atgcgctgat gtgataatgc cggatacgac gtttgcggcg tatccggctc gccgttaccg
    11221 taccaaccgc aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat
    11281 atcgcggcac agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc
    11341 aaccgcatcc agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt
    11401 ggcgcttttt ggtccgattc ccgaaacacc cggtaccttt gaactgctga tccccgcaag
    11461 tccccagtaa tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc
    11521 atccagccaa cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca
    11581 gtagccttta tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc
    11641 cagcgtggcg gctaagtcat cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg
    11701 ttgctcaaag gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg
    11761 tcgacccgct ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc
    11821 atcaaaaacg gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg
    11881 ctggcaggtt tcgacacagg gcgatccctg aacggcatga atgcgacgaa taagattcag
    11941 tgcatcgaca ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg
    12001 gtaacattcg atgccggaac aggctacggc tggatgagaa atcgggaaga ggcctcgcaa
    12061 aacgaggcct ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg
    12121 agaggtttcg cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tttcgataac
    12181 tttatcgagg cagacgcgcg gctcgctggt acggcgtagc gccatacgtg cggcgttcac
    12241 cgcttttact gccgcaatgg cgttacgctc gatgcatggc acctggacct gtccggcgac
    12301 cgggtcacac gtcagaccga ggttgtgctc catggcgatt tccgccgcga tgcacacctg
    12361 cgccgggctt gcacctaaca gttctgccag accagccgcc gccattgagc acgctacacc
    12421 aacttcaccc tggcagccga cttcagcacc agaaattgac gcgttcatct tataaagaga
    12481 accaatggca ctggctacca gcaggtaacg agccagtgag ttagcgttca cttcgcggat
    12541 aaacttatcg tagtacgcca gcacagccgg gataatcccg cacgcaccgt tagtcggcgc
    12601 agtcaccacg cgaccgccag cggcgttctc ttcgttcact gccagtgcaa acatgttgat
    12661 ccagtcaaca accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg
    12721 tagtgccgca gcacggcgtg gtacgcgcag tttgccaggc aacacgcctt cggtagaaat
    12781 accgcgctca ataccgccgc gcatgacttc ccagacgttc gccaggtgct gttccagctc
    12841 ttctttgctg tgcagcgcca gctcgttttt catcatcagg ccagagagtg acagcccggt
    12901 ttcctgacaa tgtttttgca gatcggctgc tgaactgtac ggataaggaa cttcaaccgg
    12961 tgcgctatcc tgctggccaa aatgctcttc atcaacgata aagccaccgc caatagagta
    13021 gtaagtctgg ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc
    13081 atgcagagaa aggttgtcgg cgtggaagtt catgcactga tcaaccggga actccacttc
    13141 atgctgaccg tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccggg
    13201 gatggaatcg atatccacgg tatccggcag gttacccgcc aggcccataa taatggcgat
    13261 atcagtgtgg tggcctttac cagtcagaga gagcgagccg tacacgtcaa ccaccacgcg
    13321 ggtcacgtct ttaagcaggt tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt
    13381 cattggtcca acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac
    13441 gctaatcata ggaaatacat cgcgttaaaa cggaggaagc gccgcccgaa agcggcgcga
    13501 aaggacttag ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac
    13561 aacgaatacg ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc
    13621 gtacatcggc atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat
    13681 acccaggatg ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc
    13741 agtgatacgg ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat
    13801 caccataccg ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat
    13861 agcgataatc gcgataatcg gagccatcca cgcgataacc ggtgcgttaa agtggttagc
    13921 caggtaagac agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag
    13981 gctcagtaca cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa
    14041 tgccaggatc ttcgagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac
    14101 ggcgaaagaa gagatgatcg gagagtggtt gaacgagaac accattaccg gaattgccag
    14161 ccacagggtc atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc
    14221 cagtgctgcg ccgttccact gcgggatcag gtacagagcc agcagcatca atacgccaac
    14281 aaacgggaac accagaatac tcatcgcttt aacgatcatc tgctcaccga agcgaacgat
    14341 ggtcatcata cccacgatca ggatcagcga cagaatcgca cgcggcggtg gtgtcatacc
    14401 cagctggtga gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag
    14461 gatcgggtag atagcgaaga agtagagcag ggtaatcagt ttacctgcgc caataccaaa
    14521 gtgttcttct acaacctcgg tgatgtcttc gcccgggttt ttaccagaca gtacgaagcg
    14581 agtcaggccg cggtgagcaa aaaacgtcat cgggaacgca aggatagcca tgatgatcag
    14641 cgggatcata ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc
    14701 cgtgccgtaa aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc
    14761 tttcgacgca atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa
    14821 taattcagct tttagccagt tcagcccggg aaggtgcctg accgtaaaaa tgaaatctgt
    14881 ctatcggcga tggcttaatc atttaccgcc gtaataattt gagggcggaa agatacacac
    14941 aagttatgta tctatcagtg atcttgatct caaatacaat aatctacttg gaagatagat
    15001 tatcccaacg ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc
    15061 cattaaacga catctgactt cttccggggc gcgcaatata tgtaggttga actgaaaaaa
    15121 tcaatacttt tagttaatca tggcgataat ttctttttca tagcgataaa atacggttta
    15181 tgaaaatagg gaaacgtttg cgttgcaaca taaattgtat gacatattaa ctgtgatgat
    15241 atttgtcgcg tgagggtggt aagaaacatt catataacaa atgaaatgag ttgatgagta
    15301 agtgaccaaa aacgagaaaa cccggcaaca aacgtcaccg ggggagggga gattacgtgc
    15361 agatttcgta gcaagggatg taggctgagc ctggcaactt catacgatgc tgggcaacaa
    15421 aaccctgtag caggtcgtcc atacgacgca taatctcttt atcgccgttg attttgtaag
    15481 gaccaaactc ttcaatggcg cgaataccga cttcttttac gttacccgcc acaataccgg
    15541 agaacgcacg gcgcaggtcg gcagctagca cttcaacggg ttgatccggg taaagcttca
    15601 gattagccat attctcgtga gacggctcaa acggcatttg caaatctggc gcaatgcgca
    15661 ttgaccagtt aaagctgtag gcatcgcctg tgtcgcggcg attttctttc accagcggca
    15721 tcgatttttt catctgacga gcgacttcag cggcgtcatc aatgatgatg cggtaatggc
    15781 ggcgcgcgtt ttcatccagc gtatgcacga caaactcgtc cagtacgcgg aagtagtcgg
    15841 cgctctcttt cgggccggtg aggatcaatg gtaaaacctg atctttgttg gccgggttca
    15901 ttaaaattcc cagcaaatac agcaactctt ctgccgtacc cacaccgcca gggaagataa
    15961 tgataccgtg agcgatacgg acaaacgctt ccagacgttt ttcgatatct ggcatgatga
    16021 tcaattcgtt gaccagcggg ttaggcggtt cagcggcgat aatcgacggt tctgtcatac
    16081 caataaaacg actgtctttg taacgctgct gcgcgtgtcc gaccgcagca cctttcatcg
    16141 gcgcttccat cgctcccgga ccacagccgg tgcagatatt cagctcacgc aggcccagct
    16201 ggtttccgac gcgacgggca tacaaatact cgttttcgtt aattgagtga ccgccccagc
    16261 agaccaccat atttggcgct tcacccacat gcagcgcgcg agcgttacgc aagatggaaa
    16321 agaccaggtt agtgatgtgg acggagttgt cgagattcag gttgggaaaa cgaacggtgt
    16381 tatggatttg cccgtaaacg aagagaatgt cacgcaggac cgcgaacaag ttggcctgca
    16441 aagcgcgaat aattcgccca tcgacaaaag cctcttccgg gggattaatc agttccagct
    16501 ttacgccgcg ttcacggcgc aagacgttaa tatcgaaatt ttcaaaacga gacagcaatt
    16561 ctttgctgtt atcggtcaaa ctaccggagt tcagtacggc aagtgaacag ttgcgaaaca
    16621 gttgatagag atcgctgctg gcggtgcgtt taagcatatc cacttccagc tgcgacaaca
    16681 tatccatgga gccaagcggg ctaatatgtg taatcaagaa aaactcctta tgggacgaat
    16741 atcgtcattc cctttgatta caatagccct gcctgatgac ctttcacaac cttctgcgtg
    16801 aatccagcac gcaaaattga gaaaaaattt attgccgaac cagtcttgtg gtcgatggga
    16861 caaaatcgct attactgcgc cacgggttaa tgtccagacc gccacgacgg gtataacgtg
    16921 cgtaaacgct caatttttct ggctggcaga agcgtaacag gtcattaaag atgcgttcca
    16981 cgcactgttc gtggaactcg ttgtgatgac ggaatgagac caggtaacgc agcagttttt
    17041 ctctgtcaat ttggcgtcca cgatactgaa tttggatcga accccaatct ggttgatggg
    17101 tgatcaggca gtttgatttc agcaggtggc tgacaagcgt ctcttccact actttttcac
    17161 cacaggtggc attctccaga tagtcagtag tgaattcata gttatcgata gtgatatcct
    17221 ggtcatcaat gcaagtgcca ttaaaatgac ctatcggctg gccttccagt tcatcaagac
    17281 gatataacgc cacgctaatc ttaccctgag cgcaagtgct taagtcgcgc tccagcgtct
    17341 ggcgcacctc atcccagtta ttaaaacgcg tctggttaaa actgttgaga tagagcttaa
    17401 aactcttcga ctcaatcaga tttatgctgg tgtaatcaag ttcaacatga ccgacagcga
    17461 cctgcggcaa acctttcgca ttcagccagg aaagttcata cagcgtccag atatccgtac
    17521 cgtgaaaagg caggttatcc gctttcagac ccagcgggtc gcgattcagg ctgcgtggaa
    17581 cgccttgcag taggctggcg tcataggtat cccggtaatc ggttgatttt ccaagagtca
    17641 ggcccgcaag tgcctgatgg tttgcataag aagacatgtt tcaccgtcat aaaccagata
    17701 aactaaacga caagtgtatc tggtttaagt gaagagagaa attagtggac gatttgaccg
    17761 cacaagccct gaaagatttt actgcacgct actgcgatgc gtggcatgaa gagcataaaa
    17821 gctggccgtt aagtgaggaa ctgtacggtg ttccttcacc gtgcattatt tcaactaccg
    17881 aagatgccgt atactggcaa ccccagccgt ttaccgggga acaaaatgta aacgcggttg
    17941 aacgcgcttt tgatattgtg atacaaccca caattcatac cttttatacg actcagtttg
    18001 ccggggatat gcacgcgcag tttggcgata tcaaactgac attgctgcaa acctggagtg
    18061 aagacgactt ccgtcgggtg caggaaaatc tgattggtca tctggtgact cagaaaaggc
    18121 taaaactgcc gcccacgcta tttatcgcga cgctggaaga agagttagag gtcatctcgg
    18181 tgtgtaatct ctccggtgaa gtgtgcaaag agacgctggg aacacgtaaa cggacgcatt
    18241 tagcctcaaa tcttgcggaa ttccttaatc aacttaagcc tcttctgtaa tccaaatacc
    18301 ccatctggtt gtgagagatc tcttacagat cctgtaggag atcgccagga aataagcgaa
    18361 tacttaaaaa gataagaatc gttattttca tttaaaaaca acatgttact tgttaattct
    18421 taactttcat atgaaatttt ccttaaggca ttgtctgtaa gcgccttgta agacttcgcg
    18481 aaaaagacga ttctatcttc gtcgacaggg ggtcagacaa cgaagtgaac atcaggatga
    18541 tgacacttct gcaggacgca ccaggatggt gtttcaggga aaggcttctg gatgaagcga
    18601 agaggatgac gcaggacgcg ttaaaggaca cctccaggat ggagaatgag aaccggtcag
    18661 gatgattcgg tgggtcagga aggccaggga cacttcagga tgaagtatca catcggggtg
    18721 gtgtgagcag gaagcaatag ttcaggatga acgattggcc gcaaggccag aggaaaagtt
    18781 gtcaaggatg agcagggagc aacaaaagta gctggaatgc tgcgaaacga accgggagcg
    18841 ctgtgaatac agtgctccct ttttttattc ctgctatcct tcgcggcagt ttttcttatt
    18901 gaggttgctt tatgaccact catgaccgcg ttcgtctcca gttgcaggcg cttgaagcgt
    18961 tactgcgtga acatcagcac tggcgaaacg atgaacccca gccgcatcaa tttaatagta
    19021 cccaaccgtt ctttatggac accatggaac cactggagtg gttgcagtgg gtgctgatcc
    19081 cgcgaatgca cgatttgtta aataacaacc agccgttacc gggcgctttt gctgttgcac
    19141 cctattatga aatggcgctg gcgacggacc atccgcaacg cgcgcttatt ctggctgagt
    19201 tggaaaaact ggatgctctt tttgcggatg atgcgagcta atgctggaaa tactctatca
    19261 ggatgaatgg ctggttgcgg taaataaacc ctccggctgg ctggttcacc gtagctggct
    19321 ggatcgcgac gagaaagtag tggtcatgca aaccgtgcgt gaccagatag gacagcatgt
    19381 ttttactgct catcgtctgg accgacccac ttctggtgtg ttgttgatgg gattatccag
    19441 cgaggccgga cggctgctgg cacaacagtt tgaacagcac caaatccaga aacgttacca
    19501 tgcgattgtg cgcggctggt tgatggaaga agcggtgctg gattatccac tggtggaaga
    19561 actggacaaa atcgctgata aatttgcccg cgaagataaa ggcccgcagc cagcagtgac
    19621 gcattatcgc ggtctggcga ccgtagaaat gccggtagcg accggacgtt acccgaccac
    19681 gcgctacggc ctggtggaac tggagccgaa aaccggacgc aaacatcagc ttcgccgaca
    19741 tcttgcccat ttgcgtcatc cgattattgg cgatagcaaa catggcgatt tacgccagaa
    19801 tcgcagcggt gctgagcatt ttggcctcca gcggttaatg ctacatgcca gtcagttgtc
    19861 attgacgcat ccttttactg gcgagccgct gactattcac gcgggcctgg acgacacctg
    19921 gatgcaggcg ttatcacaat ttggctggcg cggtctgctt cctgaaaatg aaagggttga
    19981 gttcagtgcg ccatcgggtc aggatggaga gataagttcc taatccaggg agaaaacggt
    20041 atggcggaaa ttggtatttt tgtcggcacc atgtacggaa attcgctgtt agtggcggaa
    20101 gaagcagaag caattctgac cgcacagggc cacaaagcaa cggtatttga agatcctgaa
    20161 ttaagcgact ggctgcctta tcaggataag tatgttctgg tggttacgtc cacgaccggg
    20221 cagggcgacc ttcctgatag cattgtgccg ctctttcagg gaatcaaaga tagtctgggc
    20281 ttccagccga atctgcgtta tggcgtgatt gcgctcggcg acagcagtta tgtaaatttc
    20341 tgcaatggcg gcaaacagtt cgatgccttg ttgcaggaac agagcgctca gcgggttggc
    20401 gaaatactgt tgattgacgc cagtgagaac ccggaaccgg aaacggaatc aaatccgtgg
    20461 gtcgaacagt ggggcacgct gttgtcctga tatgttcagc gagcggtaaa tgtcgtttta
    20521 gcggtgctga atcgaatctt ttcaggcaaa tgccagtaaa aactgcttca tagcgcggat
    20581 ttttactggc gtttgcctgg agtcaagcga tccatttcat actcttcttt atttcttcgt
    20641 tttaaccctt cctttcttgt tcttgttttc atttccgtga agtggattcc accgtccagg
    20701 gctaatgcca aaatcgggcc tcattgaacg cattaatgtt gtgttgttgc acggtgagcc
    20761 gctatggcgc gctttttata ctgctattgc cagatataaa cacgcgccgt attcggcgaa
    20821 cgacctataa aaacggcaaa aaacacccta cgtcacctct gatttcctgg ctatgtcgca
    20881 gtccagagtg agcgtggcta acgcgaattt tcaggagtgc aacaatgagt tctttaagtc
    20941 aggctgcgag cagtgtggaa aaacgcacga atgctcgtta ctggatagtg gtgatgttgt
    21001 ttatcgtcac atccttcaac tacggcgacc gcgctacgct ttctattgcc ggttcggaaa
    21061 tggccaaaga tatcggcctt gatcccgtgg gaatgggcta tgtgttctct gccttctcat
    21121 gggcttatgt tatcgggcag atccctggtg gttggttgct ggatcgtttt ggttcaaaac
    21181 gcgtctactt ctggtcgatc tttatctggt cgatgtttac cttgctgcaa ggcttcgtcg
    21241 atatctttag tggattcggc attatcgttg ccctgtttac tctgcgcttc ctggtcgggc
    21301 ttgctgaagc gccatctttc cccggcaaca gtcgcattgt tgcggcctgg tttccggcgc
    21361 aggaaagggg aacggcggtg tcgattttta actccgctca atacttcgca acggtgatct
    21421 tcgcgccgat tatgggctgg ctgacgcatg aagtgggctg gtcacacgtc ttcttcttta
    21481 tgggtggtct ggggattgtc atcagcttta tctggttgaa agtcatccac gagccaaatc
    21541 aacatccggg ggtaaatcag aaagagctgg agtacatcgc cgcgggtggc gcgctgatca
    21601 atatggatca gcaaaacacc aaagttaaag tgccgttcag cgtgaagtgg gggcagatca
    21661 aacaactgct cgggtcacgg atgatgatcg gcgtttatat cggtcagtac tgtatcaacg
    21721 ccctgactta ctttttcatt acctggtttc cggtttatct ggtgcaggcg cgtgggatgt
    21781 cgattctgaa agcgggcttt gtggcttccg ttccggcggt ttgcggtttt atcggcggtg
    21841 tgctgggtgg gattatttcc gactggctga tgcgccgcac gggatcgctg aacattgcgc
    21901 gtaaaacacc gatcgtaatg ggcatgttgc tgtcgatggt gatggtgttc tgcaactacg
    21961 tcaacgttga gtggatgatc atcggtttta tggcgctggc cttcttcggt aagggcatcg
    22021 gggcgctggg ttgggcagta atggcagata ccgcgccaaa agagatcagc ggtctttccg
    22081 gtggcctgtt caacatgttc ggtaacattt ctggcatcgt cacgccaatc gcaattggtt
    22141 atatcgttgg cacgactggc tcctttaatg gggcgctgat ttatgttggt gttcatgcct
    22201 taatcgcggt actgagctac ctggtgctgg tgggcgatat caagcgtatc gagctgaaac
    22261 ctgtcgcggg gcaataagat gacgacacaa tccagtcctg ttattactga tatgaaagtc
    22321 attccggtcg ccgggcacga tagcatgttg cttaatattg gtggggcaca taacgcatat
    22381 ttcacccgca atattgtggt actcaccgat aacgccgggc ataccggcat tggtgaagcg
    22441 ccgggcggag aggtgattta tcaaacactg gtcgatgcta ttccgatggt tctgggccag
    22501 gaagttgcgc gcctgaataa agtggttcag caggtgcata aaggtaatca ggcagccgat
    22561 tttgatacct tcggcaaagg tgcctggact tttgaattgc gcgttaatgc cgtggcggcg
    22621 ctggaagccg ccttgcttga cctgctaggt aaggcgctga atgttccggt ctgcgaactg
    22681 ttggggccag gcaagcaacg cgagactatt accgtcctcg gttatctgtt ttatatcggt
    22741 gatcggacca aaaccgatct tccttatctg gaaaatacgc cgggcaacca tgagtggtat
    22801 cagttgcgcc atcagaaagc gatgaacagc gaagccgttg tgcgtctggc ggaagcctca
    22861 caggatcgct atggctttaa agatttcaaa cttaagggcg gggtgttacc tggcgagcaa
    22921 gaaatcgaca ctgttcgtgc attgaagaaa cgcttccctg atgcgcggat taccgttgat
    22981 cccaacggtg catggctgct tgatgaagcc atttctctat gcaaagggct gaatgatgtt
    23041 cttacctatg ccgaagatcc atgcggcgca gaacagggtt tctccggtcg tgaagttatg
    23101 gcggagtttc ggcgggcgac cggcttgccc gtcgcgacta acatgatcgc caccaactgg
    23161 cgcgaaatgg gtcatgcggt gatgctcaat gcggtagata ttccacttgc cgatccgcac
    23221 ttctggacgc tttccggtgc agtccgtgtg gcgcagcttt gcgacgactg ggggctgacc
    23281 tggggctgcc attctaataa ccatttcgat atctctctgg cgatgtttac ccatgtgggc
    23341 gcggcggcac cgggtaatcc taccgctatc gatacccact ggatttggca ggagggcgat
    23401 tgtcgcctga ccaaaaatcc gctggagatt aaaaacggaa aaattgccgt tcctgatgcg
    23461 cccggtctgg gcgtggaact ggactgggaa caggtacaaa aggcacatga ggcctataaa
    23521 cgtctgcctg gcggtgcgcg taacgacgca ggtccgatgc agtacctgat ccccggttgg
    23581 acctttgacc gtaaacgtcc cgttttcggc cgtcattgat tctgaaaaag gacataaata
    23641 tgagttctca atttacgacg cctgttgtta ctgaaatgca ggtcatcccg gtggcgggtc
    23701 atgacagtat gctgatgaat ctgagtggtg cacacgcacc gttctttacg cgtaatattg
    23761 tgattatcaa agataattct ggtcatactg gcgtagggga aattcccggc ggcgagaaaa
    23821 tccgtaaaac gctggaagat gcgattccgc tggtggtagg taaaacgctg ggtgaataca
    23881 aaaacgttct gacgctggtg cgtaatactt ttgccgatcg tgatgctggt gggcgcggtt
    23941 tgcagacatt tgatttgcgt accactattc atgtagttac cgggatagaa gcggcaatgc
    24001 tggatctgct ggggcagcat ctgggggtaa acgtggcatc cctgctgggc gatggtcaac
    24061 agcgtagcga agtcgaaatg ctcggttatc tgttcttcgt cggtgatcgc aaagccacac
    24121 cgctgccgta tcaaagccag ccggatgact catgcgactg gtatcgcctg cgtcatgaag
    24181 aagcgatgac gccggatgcg gtggtgcgcc tggcggaagc ggcgtatgaa aaatatggct
    24241 tcaacgattt caaactgaaa ggcggtgtac tggccgggga agaagaggcc gagtctattg
    24301 tggcactggc gcaacgcttc ccgcaggcgc gtattacgct cgatcctaac ggtgcctggt
    24361 cgctgaacga agcgattaaa attggtaaat acctgaaagg gtcgctggct tatgcagaag
    24421 atccgtgtgg tgcggagcaa ggtttctccg ggcgtgaagt gatggcagag ttccgtcgcg
    24481 caactggcct gccgaccgca accaatatga tcgccaccga ctggcggcag atgggccata
    24541 cgctctccct gcaatccgtt gatatcccgc tggcggatcc gcatttctgg acgatgcaag
    24601 gttcggtacg tgtggcgcaa atgtgccatg aatttggcct gacctggggt tcacactcaa
    24661 acaaccactt cgatatttcc ctggcgatgt ttacccatgt tgccgccgct gcaccgggta
    24721 aaatcaccgc gattgatacg cactggattt ggcaggaagg caatcagcgc ttgaccaaag
    24781 aaccgtttga gatcaaaggc ggtctggttc aggtgccgga aaaaccgggg ctgggtgtag
    24841 aaatcgatat ggatcaagtg atgaaagccc atgagctgta tcagaaacac gggcttggcg
    24901 cgcgtgacga tgcgatggga atgcagtatc tgattcctgg ctggacgttc gataacaagc
    24961 gcccgtgcat ggtgcgttaa gacagaaaac caaaaccggc tctatctgga gccggcttga
    25021 gactgatgct aaacgcaaaa tagcctgttg tgctatgtat caggtctaca tcatttttta
    25081 attcattgaa tttaattggt tttgtaggcc ggataaggcg ttaacgccgc atccggcata
    25141 aacacaggca ctttgtcacc aatctgaaac cagcgtcata aaaagccgat tgctactcga
    25201 caagacgtcc gttacccgag aattttgctg gcttcgcgcg ctacattatc catctcgtcc
    25261 aacagttcca gcagctccgg ctccaaatct tcttctttag taccgctacg tagctgttgt
    25321 tcgatcagtt ggcagagatt cttcatacgc ggcacaccgc tatagccgca actgccatgc
    25381 agtttatgaa tcaaatcaac caggccttcc gggttttctc caaccagctg ttcctcaact
    25441 ttgttgcgaa cttcaggcag gaaatcgagt aacatttgca gcatatcgcg cgctaaatcg
    25501 gtttttcctg ctgcctggcg tagtgccagt tgccagtcga gggtcgcatt cgggttcacc
    25561 acaatttcgt tgacttcggg cgtcacgacg cgagaggaaa taccgctgcc aggcttgtag
    25621 cgcaacaaca aattatgcaa tcgctcttct tcaatcggtt tcgccagata atcgctcatc
    25681 cctgcgccaa gcagcttctc tttttgcccg gccattgcat gcgccgttac cgcgataacc
    25741 ggcgtttgtt gctgatgggg gagctggtgg atgagctcgc aggcccgaat gccatccatg
    25801 tcaggcattt gaatatccat taagatcaaa tcgaacggca tctgtttcgc ccgttcaacc
    25861 gcctgatgcc cgctatcgca aagttccaca tgttgcacca tatcttccag caatgcgccg
    25921 ataagtttca ggttagcggg gttgtcatca actgccatga ctgtcattgc cagcttgctt
    25981 tcatcggtta caggcaaaag cgtgttttgt ttgtgatgac aaaattccgt cagggcaggc
    26041 aacaggcgcg taggtgttaa tggtttcagc agacacgcgc cgataccatc ttgcttgagt
    26101 ttttcagcat tgacttgtgc atggcaagga agtgccagca tcaggaaatc ggtcatcgat
    26161 accgctttcg ctaatcgctc atgttgcatt gttagcggct cgcggaaggt caccgcgatg
    26221 cctaacaaca tcatgtcgta atgcgcggga ggcagcgcgg agaacgttgg gctataaacc
    26281 acttccagcg gcgtttcact taaaatatcc agcgtgcatt gcgctgctgc ggagtttggt
    26341 tcgacatagg ccaggcgctt acctgcgagg cactgggtgg atggcccttc gataataatg
    26401 ttcgggttca gatcgagatt aatgtggaac cagaaagttg aaccgcgatt cggctggcta
    26461 tggaacgaaa tatcgccgcc catttcatta accagttttt gtgtaatcac cagccccagc
    26521 ccggtgccac catgacggcg ggaaatactg gcatcagcct gtcggaaggc ctggaataag
    26581 cgcgattgat cgcgttcagg aataccaatg ccggtatccc gaatctgcac ttcaatctgc
    26641 actttggtat tactcagcgc acgtttttct accagaatat caatgttgcc attctcggtg
    26701 aatttaattg cattccccac caggttagtg atgatttgct gtaatcgcag tgggtcgccg
    26761 atcacgttat caggcacgtc gcttttaata ttgagcgtca gttccaaccc tttatcgtga
    26821 gaagaatgtg ccagcagagt aacgacttca tccagcgtgc tgcgtagtgg gaatggaata
    26881 ctttccagaa tcagcttacc tgcttccagt ttcgagaagt cgagaacatc attaataatt
    26941 gccagcaaat tatttgccga acgttcaatc gtattcaggt gatcgcgctg cgttggtgtt
    27001 aattctgttt tcagcgtcag gcgggtaaag ccaataacac cattcagtgg tgtacgcagc
    27061 tcgtgtgaca tatttgccag aaactcggat ttaatacgcg ccgcttcctg ggcgcgcttt
    27121 ttcgccagat ccaactcaac gttctgaatt tccatctgct ccagcgtttc acgcagatcg
    27181 gacgtcgcct ggtcgatatt gtgctgcatc tcttcgtgat aagcagccag cgacattgcc
    27241 atcgagttga taccgttttt cagcatatcc agctcgccga gcataaatcc ttccacccgg
    27301 ctgtcgagtt gcccgcgacg gatgcggtcg acggtattca ccatgttgcg aatcggaccg
    27361 gttacatcgc gcattaagcg ccagccaaaa ataagcgcaa taccgataca aaacagcatc
    27421 atcacgctgg aaataaagat ctctttatat tgctgcaagc gaaccgattt aagatccagc
    27481 tccagcgcaa tatatcccaa catattttga ctatttttgg catcgctact gggcgattcg
    27541 tcgggggagt aactctcaga aataatcggc gtgcggagga tcataatatc gccgtcacga
    27601 gtgacagtga gctggcgagg aaacggcacg ttgctgccga gctgcattga tgagggatca
    27661 agatgaaaat tggaggtgac aaagagtcgg ttattttcat cataaaccga aatcgcgcga
    27721 acaatatcgg aatggcgacg atgcagtacg cttattaact gaccgataga ttcgcgattt
    27781 tgcaggctca tgccatattc agtagaaact gcaagcggct caataatgct ggcaccggca
    27841 tcttccagtt gacgctgcaa gtcgttatag cgatgcacga tgaaaaagat actcagcaat
    27901 aaaccaataa ggacggtcgg tgccaggatc agaatcatca tgcgtgcgcg caggctgtag
    27961 ttggtcatgg agttccgtta tgggacaatt aaggtcacac tgttaaattg agaaagtctc
    28021 gccaatggcg caattctact ctgcaaaacg acgcacgacg acgcgtcaga tcataaccgt
    28081 ttcagtcaac gacctcgact cttttggtca gggcgtggcg cgacataacg gcaaaacgtt
    28141 atttatcccc ggattattgc cgcaggaaaa cgcggaagtt gctgttactg aagataaaaa
    28201 acagtatgcc cgcgctaaag tcgtacgccg gttaagcgat agcccggaac gcgaaacgcc
    28261 acgctgtcct cattttggcg tatgcggcgg ctgtcagcaa caacacgcca gcgtggattt
    28321 acagcagcga agcaaaagtg cggcactcgc ccgattaatg aaacacgaag tctctgaagt
    28381 gatcgccgat gttccctggg gctatcgccg tcgcgcgcgt ttaagtttga actacttacc
    28441 gaaaacacag caacttcaga tgggctttcg caaagcgggc tccagtgaca ttgtcgacgt
    28501 taaacaatgc cccattttag tgccccaact tgaagcattg ctgcccaaag tcagggcatg
    28561 cctgggcagc ttacaagcta tacgccatct tggtcatgtt gaactggtac aggcaaccag
    28621 cggcacgctg atgattttgc gccataccgc accgctaagt tcggctgatc gcgaaaaact
    28681 ggaacgcttt tcgcattctg aaggcctgga tctgtatctc gcccccgata gtgagatact
    28741 cgaaaccgtc tctggtgaga tgccctggta tgactcaaac gggttgcgct taacttttag
    28801 cccgcgcgat tttattcagg tcaatgcggg tgtgaaccaa aaaatggtag cgcgtgcgtt
    28861 ggaatggctg gatgtgcaac ctgaagatcg cgtactggat ctgttctgcg gtatgggcaa
    28921 ctttacactg ccattggcga cacaagctgc cagtgtggtg ggtgtagaag gtgttccggc
    28981 gctggtggaa aaaggccagc agaatgcgcg tcttaatggc ttacagaatg tgacgtttta
    29041 tcatgaaaat cttgaagaag atgtcacaaa gcagccgtgg gcgaaaaacg gcttcgataa
    29101 agtgttgctg gaccccgcgc gagcaggtgc cgcaggtgtt atgcagcaaa ttataaaact
    29161 ggaacctatt cgtatagttt atgtatcctg taatcctgca acgctggctc gggatagcga
    29221 agcgttatta aaagcaggat ataccattgc gcgactggcg atgctggata tgttcccaca
    29281 cacgggacat ctggaatcga tggtactttt ctcgcgcgtt aaatagttac gatttgccga
    29341 tttcggcagg tctggtccct aaaggagagg acgatggttg cggtaagaag tgcacatatc
    29401 aataaggctg gtgaatttga tccggaaaaa tggatcgcaa gtctgggtat taccagccag
    29461 aagtcgtgtg agtgcttagc cgaaacctgg gcgtattgtc tgcaacagac gcaggggcat
    29521 ccggatgcca gtctgttatt gtggcgtggt gttgagatgg tggagatcct ctcgacatta
    29581 agtatggaca ttgacacgct gcgggcggcg ctgcttttcc ctctggcgga tgccaacgta
    29641 gttagcgaag atgtgctgcg tgagagcgtc ggtaagtcgg tcgttaacct tattcacggc
    29701 gtgcgtgata tggcggcgat ccgccagctg aaagcgacgc ataccgattc tgtttcctcc
    29761 gaacaggtcg ataacgttcg ccggatgtta ttggcgatgg tcgatgattt ccgctgcgtg
    29821 gtcatcaaac tggcagagcg tattgctcat ctgcgcgaag taaaagacgc gccggaagat
    29881 gaacgcgtac tggcggcaaa agagtgcacc aatatctacg cgccgttggc gaaccgtctt
    29941 gggattggac aactgaaatg ggagctggaa gattactgct tccgttatct ccacccgacc
    30001 gaatacaaac gcatcgcaaa actgctgcat gaacgccgtc tcgaccgcga acactatatc
    30061 gaagagtttg tcggccatct gcgcgctgag atgaaagctg aaggcgttaa agctgaagtg
    30121 tatggtcgac cgaaacacat ctacagcatc tggcgcaaaa tgcagaaaaa gaacctcgcc
    30181 ttcgatgagc tgtttgatgt gcgtgcggta cgtattgtcg ccgagcgttt acaggattgt
    30241 tatgccgcac tggggatagt gcacactcac tatcgccacc tgccggatga gtttgacgat
    30301 tacgtcgcta acccgaaacc aaacggttat cagtctattc ataccgtggt tctggggccg
    30361 ggtggcaaaa ccgttgagat ccaaatccgc accaaacaga tgcatgaaga tgcagagttg
    30421 ggtgttgctg cgcactggaa atataaagaa ggcgcggcag ctggcggcgc acgttcggga
    30481 catgaagacc gaattgcctg gctgcgtaaa ctgattgcgt ggcaggaaga gatggctgat
    30541 tccggcgaaa tgctcgacga agtacgcagc caggtctttg acgaccgggt gtacgtcttt
    30601 acgccgaaag gtgacgtcgt tgatttgcct gcgggatcaa cgccgctgga cttcgcttac
    30661 cacatccaca gtgatgtcgg gcaccgctgc atcggggcaa aaattggcgg gcgcattgtg
    30721 ccgttcacct accagctgca gatgggcgac cagattgaaa ttatcaccca gaaacagccg
    30781 aaccccagcc gtgactggtt aaacccaaac ctcggttacg tcacaaccag ccgtgggcgt
    30841 tcgaaaattc acgcctggtt ccgtaaacag gaccgtgaca aaaacattct ggctgggcgg
    30901 caaatccttg acgacgagct ggaacatctg gggatcagcc tgaaagaagc agaaaaacat
    30961 ctgctgccgc gttacaactt caatgatgtc gacgagttgc tggcggcgat tggtggcggg
    31021 gatatccgtc tcaatcagat ggtgaacttc ctgcaatcgc aatttaataa gccgagtgcc
    31081 gaagagcagg acgccgccgc gctgaagcaa cttcagcaaa aaagctacac gccgcaaaac
    31141 cgcagtaaag ataacggtcg tgtggtggtt gaaggtgtcg gtaacctgat gcaccacatc
    31201 gcgcgctgct gccagccgat tcctggagat gagattgtcg gcttcattac ccaggggcgc
    31261 ggtatttcag tacaccgcgc cgattgcgaa caactggcgg aactgcgctc ccatgcgcca
    31321 gaacgcattg ttgacgcggt atggggcgag agctactccg ccggatattc gctggtggtc
    31381 cgcgtggtgg ctaatgatcg tagtgggttg ttacgtgata tcacgaccat tctcgccaac
    31441 gagaaggtga acgtacttgg cgttgccagc cgtagcgaca ccaaacagca actggcgacc
    31501 atcgacatga ccattgagat ttacaacctg caggtgctgg gccgcgtgct gggtaaactc
    31561 aaccaggtgc cggatgttat cgacgcgcgt cggttgcacg ggagttaggc agaaatttgc
    31621 tcgtatctac aatgtagatt gatatatact gtatctacat atgatagcgg tttgaggaaa
    31681 gggttatgat ccacagtagc gtaaagcgtt ggggaaattc accggcggtg cggatcccgg
    31741 ctacgttaat gcaggcgctc aatctgaata ttgatgatga agtgaagatt gacctggtgg
    31801 atggcaaatt aattattgag ccagtgcgta aagagcccgt atttacgctt gctgaactgg
    31861 tcaacgacat cacgccggaa aacctccacg agaatatcga ctggggagag ccgaaagata
    31921 aggaagtctg gtaatggtaa gccgatacgt acccgatatg ggcgatctga tttgggttga
    31981 ttttgacccg acaaaaggta gcgagcaagc tggacatcgt ccagctgttg tcctgagtcc
    32041 tttcatgtac aacaacaaaa caggtatgtg tctgtgtgtt ccttgtacaa cgcaatcaaa
    32101 aggatatccg ttcgaagttg ttttatccgg tcaggaacgt gatggcgtag cgttagctga
    32161 tcaggtaaaa agtatcgcct ggcgggcaag aggagcaacg aagaaaggaa cggttgtccc
    32221 agaggaatta caactcatta aagccaaaat taacgtactg attgggtagt gttactaact
    32281 cacttctatt ctggtcacag gtttccacct gacagacccg caattttcag gacattctaa
    32341 tgaatcaaat cgaccgtttg ctcactatta tgcagcgcct gcgcgatccg gaaaacggct
    32401 gcccgtggga taaagagcag acatttgcca ccattgcgcc ttacaccctt gaagaaacct
    32461 acgaagtgct ggacgccatc gcccgtgaag attttgacga tctgcgcggt gaactcggcg
    32521 atctgctgtt ccaggtggtg ttttacgcgc aaatggctca ggaagaaggg cgctttgact
    32581 ttaatgatat ttgcgctgcg attagcgata aattagagcg tcgccatccg catgtttttg
    32641 ctgacagttc tgccgaaaac agtagtgaag tgcttgcccg ttgggagcaa atcaaaaccg
    32701 aagagcgcgc gcagaaagcg cagcattcgg cgctggacga tattcctcgt agtttaccgg
    32761 ctttaatgcg tgcgcaaaaa atccagaaac gttgcgccaa cgttggcttc gattggacga
    32821 cgcttggtcc ggtagtcgat aaagtctacg aagagatcga cgaggtgatg tacgaagcgc
    32881 ggcaggctgt tgtcgaccag gctaaactgg aggaggaaat gggggacctg ctgtttgcca
    32941 cggtcaatct ggctcgtcat ttagggacaa aagcggaaat cgcattgcaa aaagcgaacg
    33001 aaaaatttga gcgtcgtttt cgcgaagtgg agcgtattgt tgccgcgcgt ggactggaaa
    33061 tgacaggtgt tgacctcgaa acaatggaag aagtctggca acaggtaaaa cggcaggaaa
    33121 ttgatctcta agggaattac gcggtcaagc gccatttgtg tcatttttta aatgacaagc
    33181 gcttgatttg cgtcaaaaac atttacccca aaggggctat tttctcactc ctgatttcaa
    33241 tagtgcgctg gcgaagagga gggataatga aagtttgtgg cacaggtcat gttcgggtat
    33301 actgctttcc cgtcctggtt attccatcgt cttttcaacc taacttctca ggttcagcat
    33361 gacaacgaac tatatttttg tgaccggcgg ggtcgtatcc tctctgggta aaggcattgc
    33421 cgcagcctcc ctcgcagcca ttcttgaagc ccgtggcctc aatgtgacca tcatgaaact
    33481 ggatccgtac atcaacgtcg atccaggtac tatgagccca atccaacacg gggaagtgtt
    33541 cgttactgaa gacggcgctg aaaccgacct ggacctgggg cactacgagc gtttcattcg
    33601 taccaaaatg agccgccgca acaacttcac cacgggtcgt atctactctg acgttctgcg
    33661 taaagaacgc cgcggtgact acctcggcgc aaccgtgcag gttattccgc acatcactaa
    33721 cgcaatcaaa gagcgcgtgc tggaaggtgg cgaaggtcat gacgtagtac tggtagaaat
    33781 cggcggtaca gtaggtgata tcgaatcctt gccgttcctc gaagcgattc gccagatggc
    33841 tgttgaaatt ggccgtgagc acactctgtt tatgcacctg acgctggtgc cgtacatggc
    33901 agcgtctggt gaagtcaaaa ccaaaccgac tcagcactct gtaaaagagc tgctctccat
    33961 cggtatccag cctgacatcc tgatttgtcg ttcagatcgc gctgttccgg cgaacgaacg
    34021 tgcgaagatt gcattgttct gtaatgttcc ggaaaaagcg gttatttctc tgaaagacgt
    34081 cgattccatc tataaaattc cgggcctgtt gaaatctcag gggctggacg attatatttg
    34141 taaacgattc agcttaaact gcccggaagc gaatctgtcc gaatgggaac aggttatctt
    34201 cgaagaagcg aacccggtaa gtgaagtcac catcggtatg gtcggcaagt acattgaact
    34261 gccggatgct tataaatcag tgatcgaagc actgaaacac ggtgggctga agaatcgtgt
    34321 cagcgtcaac atcaaactga tcgattcaca agatgttgaa acgcgcggcg ttgaaatcct
    34381 taaaggtctg gacgcaatcc tcgtacctgg cggtttcggc tatcgtggcg tagaaggcat
    34441 gattacgacc gcgcgttttg cgcgtgagaa caatattcct tatctgggca tttgcctggg
    34501 tatgcaggtg gcgttaattg attacgctcg ccatgttgcc aacatggaga acgccaactc
    34561 tacggaattt gtgccagact gtaagtaccc ggttgtggcg ctgattaccg agtggcgcga
    34621 tgaaaacggc aacgttgaag ttcgtagcga gaagagcgat ctcggcggta ccatgcgtct
    34681 cggcgcacag cagtgccagt tggttgacga tagcctggtt cgccagctgt acaatgcgcc
    34741 gacaattgtt gagcgtcatc gtcaccgtta cgaagtcaac aacatgctgt tgaaacagat
    34801 tgaagatgca ggtctgcgcg ttgcgggccg ttccggggat gatcagttgg tcgagatcat
    34861 cgaagttccg aatcacccgt ggttcgtggc ttgccagttc catccggagt ttacttctac
    34921 tccacgtgat ggtcacccgc tgtttgcagg ctttgtgaaa gccgccagcg agttccagaa
    34981 acgtcaggcg aagtaagtaa aaaagttaga acggcaacgc gtaccctggg tacgcgttgt
    35041 ttgtctggag tttcagttta actagtgact tgaggaaaac ctaatgtcca aaatcgtaaa
    35101 aatcatcggt cgtgaaatca tcgactcccg tggtaacccg actgttgaag ccgaagtaca
    35161 tctggagggt ggtttcgtcg gtatggcagc tgctccgtca ggtgcttcta ctggttcccg
    35221 tgaagctctg gaactgcgcg atggcgacaa atcccgtttc ctgggtaaag gcgtaaccaa
    35281 agctgttgct gcggtaaacg gcccgatcgc tcaggcgctg attggcaaag atgctaaaga
    35341 tcaggctggc attgacaaga tcatgatcga cctggacggc accgaaaaca aatccaaatt
    35401 cggcgcgaac gcaatcctgg ctgtatctct ggctaacgcc aaagctgctg cagctgctaa
    35461 aggtatgccg ctgtacgagc acatcgctga actgaacggt actccgggca aatactctat
    35521 gccggttccg atgatgaaca tcatcaacgg tggtgagcac gctgacaaca acgttgatat
    35581 ccaggaattc atgattcagc cggttggcgc gaaaactgtg aaagaagcca tccgcatggg
    35641 ttctgaagtt ttccatcacc tggcaaaagt tctgaaagcg aaaggcatga acactgctgt
    35701 tggtgacgaa ggtggctatg cgccgaacct gggttccaac gctgaagctc tggctgttat
    35761 cgctgaagct gttaaagctg ctggttatga actgggcaaa gacatcactc tggcgatgga
    35821 ctgcgcagct tctgaattct acaaagatgg taaatacgtt ctggctggcg aaggcaacaa
    35881 agcgttcacc tctgaagaat tcactcactt cctggaagaa ctgaccaaac agtacccgat
    35941 cgtttctatc gaagacggtc tggacgaatc tgactgggac ggtttcgcat accagaccaa
    36001 agttctgggc gacaaaatcc agctggttgg tgacgacctg ttcgtaacca acaccaagat
    36061 cctgaaagaa ggtatcgaaa aaggtatcgc taactccatc ctgatcaaat tcaaccagat
    36121 cggttctctg accgaaactc tggctgcaat caagatggcg aaagatgctg gctacactgc
    36181 agttatctct caccgttctg gcgaaactga agacgctacc atcgctgacc tggctgttgg
    36241 tactgctgca ggccagatca aaactggttc tatgagccgt tctgaccgtg ttgctaaata
    36301 caaccagctg attcgtatcg aagaagctct gggcgaaaaa gcaccgtaca acggtcgtaa
    36361 agagatcaaa ggccaggcat aagactgact ttatctgatt taaaaatgcc agcctccggg
    36421 ctggcatttt ttatggttga gttaccagcg gccggatgcg ccgccacctc cagaagaacc
    36481 gccaccgccg ctaaatccgc cgccacttga gcctccaccg gaaccgctgt tgtctga
//
LOCUS       DAJSKJ010000018        36492 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000018 DAJSKJ010000000
VERSION     DAJSKJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.018"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..2042
                     /gene="pdeF"
                     /locus_tag="ON345_000800"
     CDS             <1..2042
                     /gene="pdeF"
                     /locus_tag="ON345_000800"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR3070909.1"
                     /translation="ALGIFVRKYAELGFYETLSLTANFIIIIILCWAGYRVFTPRRNN
                     VSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQ
                     ALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLL
                     LLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIP
                     IYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRAL
                     NRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSG
                     NDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLG
                     ELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITG
                     MRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKM
                     PAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQ
                     HLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHL
                     ARMKKMRVVSEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            complement(2081..3622)
                     /gene="ppx"
                     /locus_tag="ON345_000801"
     CDS             complement(2081..3622)
                     /gene="ppx"
                     /locus_tag="ON345_000801"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR3070910.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGVSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELHLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3627..5693)
                     /gene="ppk1"
                     /locus_tag="ON345_000802"
     CDS             complement(3627..5693)
                     /gene="ppk1"
                     /locus_tag="ON345_000802"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR3070911.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(5864..6502)
                     /gene="purN"
                     /locus_tag="ON345_000803"
     CDS             complement(5864..6502)
                     /gene="purN"
                     /locus_tag="ON345_000803"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR3070912.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6502..7539)
                     /gene="purM"
                     /locus_tag="ON345_000804"
     CDS             complement(6502..7539)
                     /gene="purM"
                     /locus_tag="ON345_000804"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR3070913.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTTSAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7864..8490
                     /gene="upp"
                     /locus_tag="ON345_000805"
     CDS             7864..8490
                     /gene="upp"
                     /locus_tag="ON345_000805"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR3070914.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8576..9865
                     /gene="uraA"
                     /locus_tag="ON345_000806"
     CDS             8576..9865
                     /gene="uraA"
                     /locus_tag="ON345_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR3070915.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            9915..10661
                     /gene="hda"
                     /locus_tag="ON345_000807"
     CDS             9915..10661
                     /gene="hda"
                     /locus_tag="ON345_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR3070916.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(10799..11158)
                     /gene="arsC"
                     /locus_tag="ON345_000808"
     CDS             complement(10799..11158)
                     /gene="arsC"
                     /locus_tag="ON345_000808"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR3070917.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11179..12642)
                     /gene="bepA"
                     /locus_tag="ON345_000809"
     CDS             complement(11179..12642)
                     /gene="bepA"
                     /locus_tag="ON345_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR3070918.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            12855..13916
                     /locus_tag="ON345_000810"
     CDS             12855..13916
                     /locus_tag="ON345_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3070919.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(13954..14802)
                     /locus_tag="ON345_000811"
     CDS             complement(13954..14802)
                     /locus_tag="ON345_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR3070920.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIISGAVLVSICYRAI
                     YLRQES"
     gene            complement(14824..16836)
                     /gene="hyfR"
                     /locus_tag="ON345_000812"
     CDS             complement(14824..16836)
                     /gene="hyfR"
                     /locus_tag="ON345_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR3070921.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(16866..17279)
                     /gene="hyfJ"
                     /locus_tag="ON345_000813"
     CDS             complement(16866..17279)
                     /gene="hyfJ"
                     /locus_tag="ON345_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR3070922.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSMQFIHLLDEIVQEPAIYLMARKIT"
     gene            complement(17272..18030)
                     /gene="hyfI"
                     /locus_tag="ON345_000814"
     CDS             complement(17272..18030)
                     /gene="hyfI"
                     /locus_tag="ON345_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR3070923.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18027..18566)
                     /gene="hyfH"
                     /locus_tag="ON345_000815"
     CDS             complement(18027..18566)
                     /gene="hyfH"
                     /locus_tag="ON345_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR3070924.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVLLVAKEQL"
     gene            complement(18576..20291)
                     /gene="hyfG"
                     /locus_tag="ON345_000816"
     CDS             complement(18576..20291)
                     /gene="hyfG"
                     /locus_tag="ON345_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR3070925.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSVLVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVTVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20281..21861)
                     /gene="hyfF"
                     /locus_tag="ON345_000817"
                     /pseudo
     CDS             complement(20281..21861)
                     /gene="hyfF"
                     /locus_tag="ON345_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            complement(21866..22516)
                     /gene="hyfE"
                     /locus_tag="ON345_000818"
     CDS             complement(21866..22516)
                     /gene="hyfE"
                     /locus_tag="ON345_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR3070926.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22528..23843)
                     /gene="hyfD"
                     /locus_tag="ON345_000819"
                     /pseudo
     CDS             complement(22528..23843)
                     /gene="hyfD"
                     /locus_tag="ON345_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(23855..23965)
                     /locus_tag="ON345_000820"
     CDS             complement(23855..23965)
                     /locus_tag="ON345_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR3070927.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(23982..24929)
                     /gene="hyfC"
                     /locus_tag="ON345_000821"
     CDS             complement(23982..24929)
                     /gene="hyfC"
                     /locus_tag="ON345_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR3070928.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKHQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(24940..26956)
                     /gene="hyfB"
                     /locus_tag="ON345_000822"
                     /pseudo
     CDS             complement(24940..26956)
                     /gene="hyfB"
                     /locus_tag="ON345_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(26956..27573)
                     /gene="hyfA"
                     /locus_tag="ON345_000823"
     CDS             complement(26956..27573)
                     /gene="hyfA"
                     /locus_tag="ON345_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR3070929.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(27826..28296)
                     /gene="bcp"
                     /locus_tag="ON345_000824"
     CDS             complement(27826..28296)
                     /gene="bcp"
                     /locus_tag="ON345_000824"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR3070930.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDDLKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28296..28868)
                     /locus_tag="ON345_000825"
     CDS             complement(28296..28868)
                     /locus_tag="ON345_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR3070931.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29014..29892
                     /gene="dapA"
                     /locus_tag="ON345_000826"
     CDS             29014..29892
                     /gene="dapA"
                     /locus_tag="ON345_000826"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR3070932.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            29909..30943
                     /gene="bamC"
                     /locus_tag="ON345_000827"
     CDS             29909..30943
                     /gene="bamC"
                     /locus_tag="ON345_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR3070933.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYNTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            31156..31869
                     /gene="purC"
                     /locus_tag="ON345_000828"
     CDS             31156..31869
                     /gene="purC"
                     /locus_tag="ON345_000828"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR3070934.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32024..32251
                     /gene="vapB"
                     /locus_tag="ON345_000829"
     CDS             32024..32251
                     /gene="vapB"
                     /locus_tag="ON345_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR3070935.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32251..32649
                     /gene="vapC"
                     /locus_tag="ON345_000830"
     CDS             32251..32649
                     /gene="vapC"
                     /locus_tag="ON345_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR3070936.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32796..33659
                     /locus_tag="ON345_000831"
     CDS             32796..33659
                     /locus_tag="ON345_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR3070937.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33674..35689
                     /gene="tmcA"
                     /locus_tag="ON345_000832"
     CDS             33674..35689
                     /gene="tmcA"
                     /locus_tag="ON345_000832"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR3070938.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35763..36461
                     /gene="ypfH"
                     /locus_tag="ON345_000833"
     CDS             35763..36461
                     /gene="ypfH"
                     /locus_tag="ON345_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3070939.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
BASE COUNT         8886 a         9390 c         9673 g         8543 t
ORIGIN      
        1 tcgccttagg gatttttgtc cgcaaatatg cagagctggg tttttacgaa acgctatcat
       61 taacggctaa ttttattatc attatcattc tctgttgggc cggttacagg gtctttactc
      121 cccggcgtaa caacgtttca catggtgata cccgtttaat ttcccagcgt atattctggc
      181 agattgtgtt tcctgcaacg ctgtttctga tacttttcca gtttgctgca tttgtaggat
      241 tactggcgag cagagaaaat ctggtcggtg tcatgccttt taacctcggg actttaatca
      301 attatcaggc cttactggtg ggtaatctga tcggtgtccc gctgtgctac ttcatcattc
      361 gggtagtgcg aaatccattt tatttacgta gctattattc gcaattaaaa cagcaggttg
      421 atgccaaagt caccaaaaaa gagttcgcgc tctggctact ggcattaggt gctttattgt
      481 tgctgttatg catgccgtta aatgaaaaaa gcacaatttt tagcaccaat tataccttgt
      541 cattattgct gcccctgatg atgtggggag cgatgcgcta tggttataag ctgatttcgc
      601 tgctctgggc ggtcgtgttg atgatcagca tccacagcta tcaaaattac attcccattt
      661 atcctggcta taccacgcag ctgaccataa cctcctccag ttatctggta ttctctttta
      721 ttgtcaatta tatggctgta ctggcaaccc gtcagagagc ggtagtcaga cgcattcagc
      781 ggcttgcgta tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt
      841 tgcgtgatgc cccctggtct gcgctttgtt atttacgcat ccctggcatg gaaatgctgg
      901 ttaagaacta tggcatcatg ctgcggattc aatacaagca aaaactttct cactggctgt
      961 cacccttgct ggaaccgggt gaagatgttt atcagctttc gggtaacgat ctcgcgctgc
     1021 gactgaatac agaatcgcac caggagcgaa ttaccgcact ggatagccat ctcaagcaat
     1081 ttcgtttctt ttgggatggc atgccgatgc aaccgcagat tggcgtcagt tactgctatg
     1141 tgcgctcgcc agtgaatcat atctacctgc tgctgggaga gctaaatacg gtcgccgaac
     1201 tttccatcgt gaccaacgcc ccggaaaata tgcagcgtcg cggggcaatg tatttgcaac
     1261 gcgaattgaa agataaagtc gcgatgatga atcgactaca gcaggcgctg gaacacaacc
     1321 attttttcct gatggcccag ccgattaccg gtatgcgtgg tgatgtttac catgaaattc
     1381 ttctgcgcat gaaaggtgag aatgatgaac tgatcggccc cgatagcttc ttgccggtcg
     1441 cgcacgaatt tggtttatcg tcgagtatcg acatgtgggt cattgagcat acgctgcaat
     1501 ttatggctga aaacagagcg aagatgcccg ctcaccgttt tgctattaat ctgtctccaa
     1561 cctcggtatg tcaggctcgt tttcctgttg aagtcagtca gttgctggct aaatatcaga
     1621 ttgaagcgtg gcaacttatt tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc
     1681 aggcgcaaat caccttgcag catcttcagg aattaggctg ccagattgcg attgatgatt
     1741 tcggcaccgg ctacgccagc tatgcacggc ttaaaaatgt gaatgccgat ctgcttaaaa
     1801 ttgacggcag ttttatccgc aatattgtgt caaatagtct ggattatcag atagtggcat
     1861 cgatttgcca cctggcgcga atgaagaaaa tgcgggtagt gtcagagtac gttgaaaacg
     1921 aagagatccg cgaggcggtg ctctctttgg ggatcgatta tatgcagggt tatcttattg
     1981 gtaagccgca gccgttaatt gatacgttga atgaaatcga acccattcgc gaaagtgcct
     2041 gaataatgcg ggccgacatt tctcgtcggc ccgcaaagca ttaagcggcg atttcaggtg
     2101 tactttcttc ttcaattttc aaccgccagc cagccacgcc ttcccagtat tcttgctcct
     2161 tttccagatc aagcagtacc aacgcattct gactaaacca gtcatgcggg aaacgcagtg
     2221 tccagtgact gtcatcagta atcagtgtca atgttggcgg tgtggtggtt gcctgacgtt
     2281 gattgttgag taatacgcca aggcgcaata gctgaatcag tggcaggaac tgtttcttct
     2341 taaacaaggt aaagcgcggc agatcgtcga gtttaatcgc tttacggtga tagcgcacca
     2401 gtgtcgccat catcagctgc tgttcctgat taaaacccgg caagtcactg ttttgcagaa
     2461 tataagcgga gtggcgatgc aaaccgctgt ggttgatatt caacccgacc tcatgcagca
     2521 tggcggccca tcgcagtagc gcctccagtt gcggatgcgc cagcttcggt tgctgttccc
     2581 gccactgttc gtacatttgc atagtggtat ccagcacccg tcgggcctgt tcgctgtcga
     2641 tgtgatactg gttggcgagg ctgctggcgg tgcgactacg cacatcctga tgacggaaac
     2701 gtccttccat ttcgtacagt acgccttcgc gtaacgcccc gtcagaaagg tgcagttcac
     2761 ggatggctaa agcatcaaac acaccgcata aaatcgccag tccaggaacg aagactgttt
     2821 tccgctcttc ggaaagaccc ggtaaactca gcgatgcgaa attacggtga cgtaaaactt
     2881 cttttaccag tttttccaga cgttccgggg taattatccc gtctttctcg cccatttcca
     2941 ttaacacttc atgggcggct tttatggtac cggaagcgcc cattgccacg ttccagccct
     3001 gaatacggaa ttgccaggtt aaagtttcca gtttttgtgc tgccgccata cgagcgcgct
     3061 gaaaattctc tttattgatg accccgccag ggaagtaaag ctgggcaaag ctgacacaac
     3121 ccatccggcg gctttcaacg agaatcggtt cgaaattttc accaatcacc agttccgtag
     3181 atccgccgcc aatatcaata accagtttgc gacctttctc cggttgggta tgttccacgc
     3241 ccataaaaat cagacgggct tcttcattac cggaaataat ttcaatcggg tagggaatga
     3301 ccttttccgc gcgtttcaga aagtcagtgg cgttcagcgc ctgacgcagc gtatgggtgc
     3361 cgactataca gacgctggca ggagaaaccc cttgtagccg ttcggcaaac agcgacagac
     3421 agttcaaacc gcgcgtcatt gcctcttcac tcaacatatt atctggcccc aggccgtccg
     3481 ccagatgcac tcgctgtttc aggcggccaa taatctgcat ggcaccatct accacacggg
     3541 ctatgaccat gtgaaaactg tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag
     3601 gggatttatc gtgtattggc atagggttat tcaggttgtt cgagtgattt gatgtagtcg
     3661 taaatcgcca actgcgcccg tactttgcgg cgattgccgc ggggaacgta gcgattactg
     3721 agttctttat cgatataacg tgctttgacc gtatcgctga acaatatgtc gatgatgtcc
     3781 agcacccgct gcttcaggcg cggatcgagc agcggtgtcg ccacttcaat acgataatca
     3841 atattgcgcg tcatccagtc ggcggaagaa aggtagacct ttttatcgcc gccattttca
     3901 aaaatataaa cccggtcatg ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg
     3961 tcgctaatgc cttccagatt ggggatcagc gaacacattc cgcgaaccag cagattaacc
     4021 ggtacgccgg agctggaggc cgcatacaga cgatcaacca ggcctttatc gacaaggtta
     4081 tttagcttca gggtgatacc actgggcagc ccttgctgcg cgttggcgat ttcgcggtct
     4141 accatttcat ataacagacg gcgagagttt tgcggtgaca ccattaaata atcaaatgtc
     4201 actgggcggt atgggttttc aataaagtta aatacccgcc gtacttcgtt ggtgatacgc
     4261 gcatctgcgg tcagcaacga atagtcagta taaagacgcg cggttttttc gttaaagttc
     4321 ccggtcccga tgtgtgcgta acggaccact tcaccgtttt ctttacgtga aatcaggaac
     4381 agtttggcgt gaattttcag ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc
     4441 aggcgcttcg cccagtgaat gttggcttct tcgtcgaaac gcgcctgtaa ctcaaccacc
     4501 acagtgactt tcttaccgtt atgtgcggcg tggatcatcg agtcgatgat gcgtgaatct
     4561 ttcgccacgc ggtaaatgtt aattttaatc gccagtacgc tcgggtcgaa cgaagcctga
     4621 cgcagcagtt ccagcacatg ctcaaaggtg tgataaggat aatagagcaa cacatcgcgt
     4681 tcgcgaatgg catcaaaacc attgcggaac tgggctttat caaaccaaat gtggcgtaaa
     4741 cgcggcagtg gtttgttcac cagattggct ttgccgacat tggggaaatt aataaagtct
     4801 ttaaaattat gataacgacc gccggggacg atggagtcgt agcgggaaat agtcagtttt
     4861 tcgcgtaaca cttcaaccag cgcattgggc atatcgcgct gataaacaaa acgcaccggc
     4921 tcagcagtta aacgctgctt gagactggaa gacatcaact ccatcaggct ggcttccatc
     4981 tcatgcacta aatcgtattc ggcatcgcgg gtcatcttca ttgaataggc attcagcgcg
     5041 tcataatcaa agaagccttt gaaaatatca tcaaggcagt aacgcagaat gttatccaga
     5101 agaatcatcg gcttgcgtcg acgcggcgct tctggcggta aattcacaaa gcgcggcact
     5161 ttatctgatg ggatctccag cagcgcgtaa cggatggtat caccacggat aatttccacc
     5221 gccagatagg tgtaatcatc cttcaggaac tgcactaagt cagtgtcagg attgattaaa
     5281 atcggcgtaa tgtgctgacg cagatactgc ttaaaataat gacgaagcca gttttgttga
     5341 ttgacggaga gttggcgttc attaatcagg aagatctggt tgcgcgccat ctccagcaat
     5401 agctcgttgt agaggccgtc gaattcctga tcggctttca gcacccggga ctgaattttg
     5461 cccagtaaat ggcgggaatg agagttggag ccttgttctt cgctaataat gatgcgtcgc
     5521 ttcagttcag cgaagcggac tttatagaac tcatcaaggt tattggaata gatccccagg
     5581 aaacgcatcc tttcaatcag cgggttagat ttgtccgccg cttcctgaag cacgcgttca
     5641 ttgaacgata accaactgag ctctttttcg atgtatagct tttcctgacc cattaccact
     5701 tttactccgt tttattcacg ggacactgcc tggatattat ggcgagcaat attggcagat
     5761 gtccaactat gccagaaaag ggggaaaaaa actgctggag cttgcgattt acaggtgaat
     5821 tgaaaaacgc cagcggcaga gctggcgctt taactacggg gcattactcg tcggcagcgt
     5881 agccctgcgg cggcagacgt tgaccatcca gccacgcggc gttttcgtgc attttcagac
     5941 gaccatcggc aaaccagcta atcaccagtg gataaatagc gtgttcctgg gtttgcacgc
     6001 gggcggtgat gtcatcttcc gtatcaccag caaataccgg gactttcgcc tgtaaaataa
     6061 ccgggccacc gtccagttca tcggtgacga aatgcaccga tgtaccgtgc tcttcatcac
     6121 cattttccag cgcctgacga tgggtgtgta atccgggata tttcggcagc agagaagggt
     6181 gaatgttcag caaacgcccg gcatagtggg agacaaacgc cgggctgaga atgcgcataa
     6241 aaccagccag cacgaccaca tcgggtgcgt acatgtcgat ttcatgaatc aactcccggt
     6301 cataggcttc acgactgtca aacgcgctgg cgatgagcgt atgcgttgca atacccgcct
     6361 ggcgggcgcg ttcaaggccg aacgcgtcgg ccttattgct gaaaactgcc cgtacggtgc
     6421 ctttaatttt gttggtttta caggcgtcaa taattgcctg taaattactt ccgttgccgg
     6481 aaataagcac cacaatattc attattcgat aaccacgcgt tgttcggaat cagaggcttt
     6541 gatgataccg attttccacg cgttttcacc gttggcattt agcagggcga gggctttgtc
     6601 cacttccgga gcaggcaggg cgataatcat cccgacgccg cagttgaagg tgcgatacat
     6661 ttcatggcgc tcaacgttac ctgccgtttg cagccagttg aacacttccg gccactgcca
     6721 ggaagattca tcaatcactg cctgagtatt atctggcaat acgcgtggaa tgttttccca
     6781 gaagccgccg ccggtcaggt gcgcaatggc atgcacatcg accttttcaa tcaactccag
     6841 cactgacttc acgtaaatgc gggtcggtgc cagcagatga tcggctaatg gcttaccatc
     6901 aagttcggtg gtttgcggat cacaaccgct gacttcaaga attttgcgca ccagcgaata
     6961 gccgttcgag tgcggaccgc tggaaccgag tgcaatcaga acatcgccgt cgctgacttt
     7021 agagccgtcg atgatttctg atttttctac cacgcccacg cagaaacccg cgacatcgta
     7081 atcttcaccg tgatacatcc ccggcatttc tgccgtttcg ccacccacca gtgaacagcc
     7141 cgattgcaga caaccttccg cgatgccgct gatcaccgct gaagtggtat caacatccag
     7201 ttttccggtt gcgtaatagt cgaggaaaaa cagcggttct gcaccttgca ccaccaggtc
     7261 attaacgcac atggcgacca gatcaatacc aatggtgtcg tgacgtttta agtccattgc
     7321 cagacgcagc ttggtaccta cgccgtcagt gccggaaacc agcacgggtt cacgatattt
     7381 ttgcggcaat gcacacagcg caccgaagcc gcccagaccg cccatcactt ccggacgacg
     7441 cgttttcttc actacgcctt tgattcttcc aaccagagca ttacccgcgt caatatcaac
     7501 accggcatct ttgtagctaa gagaggtttt atcggtcact gcttgggtcc ccacgcgtta
     7561 cttgcggtag aaaaataaaa ttcggcgcaa ttctaacagg gaaagcaaac gtttgcgaga
     7621 ctgctttgca caaccttttt gcacgtcttt tccccagacg cgcggcgaaa gaagacttgt
     7681 gccagggcaa aggttagttt tcggatggaa tgatcttctt tcataaccat ttgaatataa
     7741 aataacttta tctcaaatcg ttatcatttt gactaaagtc aacgaaaaga atattgccgc
     7801 cttgaagaaa ggaggtataa tccgtcgatt ttttttgtgg ctgcccctca aaggagaaag
     7861 agtatgaaga tcgtggaagt caaacaccca ctcgtcaaac acaagctggg actgatgcgt
     7921 gagcaagata tcagcaccaa gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg
     7981 acttacgaag cgaccgccga cctcgaaacg gaaaaagtaa ctatcgaagg ctggaacggc
     8041 ccggtagaaa tcgaccagat caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg
     8101 ggtcttggta tgatggacgg tgtgctggaa aacgttccga gcgcgcgcat cagcgttgtc
     8161 ggtatgtacc gtaatgaaga aacgctggag ccggtaccgt acttccagaa actggtttct
     8221 aacatcgatg agcgtatggc gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt
     8281 atcgcgacca tcgacctgct gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg
     8341 gtcgctgcgc cagaaggtat cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat
     8401 accgcatcga ttgatcaggg actgaacgag cacggataca ttattccggg cctcggcgat
     8461 gccggtgaca aaatctttgg tacgaaataa agaataaaaa taattaaagc cgactttaag
     8521 agtcggcttt tttttgagta aagcgcctat aacacataat acagaggata atactatgac
     8581 gcgccgtgct atcggggtga gtgaaagacc gccactttta cagacaatcc cgcttagttt
     8641 gcaacatttg ttcgccatgt ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa
     8701 cccggcgact gtactgttat ttaacggtat tggaacgctg ctgtatctct tcatctgtaa
     8761 agggaaaatt ccggcttatc ttggttccag ctttgccttt atttcaccgg tattgttact
     8821 gttgccgtta gggtatgaag tcgcgctggg cggctttatt atgtgcggcg tgctgttctg
     8881 cctggtttct tttatcgtga agaaagcggg gaccggctgg ctggacgtgc tgtttccacc
     8941 tgcggcaatg ggcgcaatcg ttgccgtcat cggtctggag ctggcgggcg tagctgccgg
     9001 tatggcgggt ttactcccgg ctgaagggca aacgccagac tccaaaacca tcatcatctc
     9061 tattaccacc ctggcggtca cggttttagg ttccgtgctg tttcgtggtt tcctggcaat
     9121 tatcccgatt ttaattggcg tgctggtggg ctacgcgctc tctttcgcaa tgggaattgt
     9181 cgataccacg ccgattatta atgctcactg gtttgcgctg ccaaccctct atacgccgcg
     9241 cttcgagtgg tttgccattc tgactattct gccagcggcg ttagtggtta ttgccgaaca
     9301 cgtagggcac ctggtagtaa cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg
     9361 tctgcaccgt tcgatgtttg ctaacggcct gtcgactgtg atctccggct tctttggctc
     9421 cacgccaaat accacttacg gagaaaacat tggcgtgatg gcgatcaccc gtgtttacag
     9481 tacctgggtt atcggcgggg cggcgatttt cgctatcctg ctttcctgcg tcggtaaact
     9541 ggctgccgct atccagatga tcccattgcc ggtgatgggc ggcgtttcgc tgctgcttta
     9601 tggtgtcatc ggtgcttccg gtattcgtgt tttgatcgaa tcgaaagtgg attacaacaa
     9661 agcacagaac ctgatcctga cttccgtgat tttgatcatc ggcgtcagtg gcgcgaaggt
     9721 aaacatcggc gcggcggagt taaaaggtat ggcgctggcg accatcgtcg gtatcggctt
     9781 aagtttgatt ttcaaactga tcagtgtgct gcgccctgaa gaagtggtgc tggacgcaga
     9841 agacgcggat ataacagaca aataatgtac cgaccgggca gtgttgctgc ccggttcaaa
     9901 catcatggga ttctgtggta aacttctcgc gattttgtga aatcctggtt gaggtatctc
     9961 tgaacacacc ggcacagctc tctttgccac tttatcttcc tgacgacgaa acctttgcaa
    10021 gtttctggcc gggggataac tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg
    10081 aacatagcgg ttacatctat ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc
    10141 acgcggcttg cgcggaattg tcgcagcgtg gcgatgcggt gggctatgtc ccgctggata
    10201 aacgcacctg gtttgttccg gaagtgctcg acggtatgga gcatttgtcg ctggtctgta
    10261 tcgacaacat tgagtgtatt gcaggcgatg agttgtggga gatggcgatt ttcgatctct
    10321 acaatcgaat tctggaatcg ggcaaaacac gattgttgat caccggcgat cgtccaccgc
    10381 ggcagttgaa tctgggatta ccggatctcg cttcgcgact cgactggggg cagatctaca
    10441 aattgcagcc actttctgat gaagataagt tgcaggcgct acagttacgc gcgcgtttgc
    10501 gtggttttga actgccggaa gatgtggggc gtttcttgct gaagcggctc gacagagaaa
    10561 tgcgcacgct atttatgacg ttggatcagt tggatcgtgc gtcgattacc gcgcaacgta
    10621 agctgaccat tccgtttgtg aaagaaattc tgaagttgta gataaggtgt ttattgtcgg
    10681 atgcggcgcg aacgccttat ccgaactacg cgaagttaat ccatagtatt cgtaggccgg
    10741 ataagacgtg gcaagcgtcg catccggcaa tgtgcttcaa ctagcgatgg agcacatctt
    10801 aacccacgat ttccagtacc tgctccggtg gacgaccaat ccgcgctttg ccattcgcga
    10861 ccacaatcgg gcgctccatt agcttcggat tatccaccat tgcctgaatc agcgcctctt
    10921 cgctaagcga actgtcagcc aggttcagtt ctttataaag atcctctttc tggcgcatca
    10981 attcacgggc gctattcatc cccagcattt tcagcaaatc gcgcagcgtt gccgcatcgg
    11041 cgggtgtctc aaggtagagc accacttctg gctccacgcc gttttctttc agcaggttca
    11101 gcgtttcccg gctctttgaa cagcgtgggt tatggtagat ttttacctgt ttggtcattg
    11161 ttcttccttt aatgcgaatt acatcttggt ataaggctta aagcgttcct gcagctggcg
    11221 caactggtcg atgcgcgcat cgtaacgcgc ttgttgcagg ctgcctaatt tcacctgcga
    11281 actggcgcta ctcaacagcg aaatggcctg atcgagtcgc ccggcgagcg cataaccttc
    11341 tgctcgcgca gccagttcct gatcgcggtt atttagcgcg gcttccgcct gtgccagtaa
    11401 atcccagccg ttgctgtcat ctttattgtt aaaagtgtag cgattgagaa tattggccgc
    11461 ttcttgtggt tgaccgcctt gtagataagc gttcgccagg ttgagctgca acaccggatt
    11521 ggtgcgcaaa tcacgggcat ttttcaggcg attgattgcc tcattggctt tgttttgccc
    11581 aagatcgata tcggtagcca gatcgagata ccatgcgttg ccaggttctg ccgccagtaa
    11641 cggttgcagc gttttacgcg cctcgtcgta tttattggct tccatcgcct gtaaagcacg
    11701 accatattgc gccgctcgtt gctgacgaac gtttcctttc gcccattcat ccagcaaatc
    11761 actggtgagc tggttacgtc cggaattata catccccagt gtgcgcgctt tcgccagata
    11821 gaaatcttcc gacgactgca ccaccatcgg tcgcatctga ttagcacggt tgcgggcatc
    11881 tgccagacga ctttccggca acgggtgagt caataaaatt tccggcgggc gcgaggagta
    11941 acgcgcctga tcgagtaatt tttcgaggaa ggttggcatc gcctgcggat cgaatcccga
    12001 gcgttgcagt acctgaatac caatgcggtc cgcttcctgt tcattttgct gggtgaaact
    12061 gatcatcccc tgacgtgttc ccgccagtgt accggtcagc gccgccatcc ccgcctgcgg
    12121 actggccatc gccagtaaaa tagaacctaa cgcgccgacc caggtcagcg gcgcgctgcg
    12181 ctgctgatct tccatcgctc gcgccaggtg acgttgggtg acgtgggaga tttcgtgcgc
    12241 cataactgaa gccagttgac tttcgttatc ggaataacgg aacagggcag agtgcagcac
    12301 cacgttgccg ccaaagaaag caaaggcgtt aatttcgtcg ttgttgatca gaaaaaaatg
    12361 aaacggtgtc ttaaccgaat tggcatgcga aaccagacgc atccccagcg aattaatata
    12421 ttgcgttaac agcgggtcat taattaacgg cgcgctgccg cgtagctggc ggacataata
    12481 gtcgcccatc tgcatttcct gaccaatgga aagcgtgctt cctgcggagg ttcccatatc
    12541 cggcaaggtg tctgcgttgt cggcaaacgc cggggctacc tgaccaatgg tcatagcagc
    12601 aatgagggtt gcaaccaggt tttttttcaa ctgcctgaac ataacctcta tcctgtattt
    12661 ctgaagatag ttatttgacc gatactgtga aatatcgttc ccaccgcgcc gcctttgagt
    12721 gtaacggcga agattagccc actcaaatga cttttcagtt tcctgaatcc gtcataaaaa
    12781 gcgaagtctt ttttgtgaca tttcgataca attgcggatc gcaatcccgc tcttgaggtt
    12841 cagggaaggt ttttatgctc gaaatgttga tgcaatggta tcgccgccgt tttagcgacc
    12901 cggaagcgat tgctttgctg gttattttag ttgccggatt tggcattatc tttttcttta
    12961 gtggcctgct tgctccgttg ctggtggcta ttgtgctggc ctatttgctg gaatggccaa
    13021 ccgtgcgcct gcaatctatt ggctgctccc gccgctgggc gacgtcgatt gtattggtgg
    13081 tttttgtcgg tatattgcta ctgatggcgt tcgtggtact gcctatcgcc tggcaacagg
    13141 gcatctactt aatccgcgat atgccgggga tgctcaataa gctttctgac tttgccgcca
    13201 cgttgccgcg ccgctatccg gcgttaatgg acgcgggcat tattgatgca atggccgaaa
    13261 atatgcgcag tcggatgctg accatgggcg attcggtggt gaaaatttcc ctcgcctcgc
    13321 tggtcggttt gctgaccatt gccgtctatc tggtgctggt gccattgatg gtcttcttcc
    13381 tgctgaaaga caaagagcag atgctgaacg ccgttcgtcg ggtgctgccg cgcaaccgtg
    13441 gactggcagg acaggtgtgg aaggagatga atcaacaaat caccaactat atccgcggca
    13501 aagtgctgga gatgatcgtg gtggggatcg ccacctggct ggggttcttg ctctttgggc
    13561 tgaactattc gctgctgctg gcggtgctgg tcggcttctc ggttcttatt ccgtacattg
    13621 gcgcatttgt ggtgaccatt ccggtggttg gcgtggcgct attccagttt ggcgcaggca
    13681 cggaattctg gagctgcttc gcggtgtatc tgattattca ggcgctggac ggcaacctgc
    13741 tagtaccggt gttgttctcc gaagcggtta acctgcatcc gctggtgatt attttatcgg
    13801 tggtgatctt cggtggtttg tggggattct ggggcgtatt cttcgccatt ccattggcga
    13861 cgctgatcaa agccgtgatc cacgcctggc ccgatgggca aatcgcgcaa gaataatctt
    13921 cagcccgacg aactgtttcg ccgggctgat ttttcaggat tcctgacgta aataaatagc
    13981 ccgataacaa atactcacca gcactgcacc gctgataata ttacccagca tcaccgggag
    14041 cagattggcg gtaataaaat ggctgaccgt cagtgccgga aaattgtctg cgctactgtg
    14101 cgccagttgc cagaaggggg gaggggcgaa atggcgaatg gcaatggcga acggaatcac
    14161 aaacaaattg gcgatacagt gctcaaagcc actggcgaca aacagggtga tgggcaaaat
    14221 catggcgacg attttgtcgc ataacgaacg cccgcaataa ctcatccaca gcgccaggca
    14281 aaccatcaga ttgcacataa tgccgaggct gacagattca gtaaatgtat gatgcatttt
    14341 gccctcggcg cagtgtaaaa ccgcgactcc ccacatcgcg ttttcactca tcaccagccc
    14401 ggaaaaccag attaacaaac tgaaacaggc aatacctgcc agattaccgc aggccaccag
    14461 aagtgcgtta atcagccaag ttcgccaact aataacgccc cgactttttg ccattaccgt
    14521 cattaccgac gaggtgaaca ggttggtgcc gcaaaccgcc agcaaaataa agccaagtgt
    14581 aaagcaaagg ccgcccacca gatgggttaa tgcctgtgaa gacggggcat cggcgataac
    14641 tgaaaggtaa aaagtaaatc cgatcgccat aaacacgcca gcggacatcg ccattaaaaa
    14701 gaccgacact ttactgcggg caattttatg ggcggcaatc cgttcagcga ttgccgcttt
    14761 tctggcgctc aactgcaagt cgaaagagag tttgtttctc atgcatactc ctgcaaaagc
    14821 agattacaac acctcgcgaa ccgagattcc cagacgctgc attcgtgaca gcagcgtggt
    14881 gcgcttcatc cctaatcgtg tcgccgcgcc acggggcccg gcaacaatgc cattggtttc
    14941 tcgcaatacc tgaacaatgc gctgacgttc ttcttcgtca ttctctggcg tcgtcgggtg
    15001 cagcaactgc gccattgaac tgcgaagcgc tgaatcttca tttagcgtcg gcagtaaacg
    15061 gctttgtcgg acattaagat gtaaattcag actgttaccg cgagtcagca gtaccgctcg
    15121 ctcaatcacg ttttccagct cgcgcacgtt gcccggccag tcccacgaca tcaactggcg
    15181 tagcgcctcg gtcgggatgg cgtcaattga acgattcata tggcgcgcca ttttttgcgt
    15241 gaaatgcttt gctaaaagag ggatatcttc cggacggtcg cgcagcggcg gcaattccag
    15301 tgggaagaca ttcaggcgat aaaagagatc gctgcgaaac tggcgatctt caaccatttg
    15361 ccacaaatca cggttggtgg cggcaatgac tctgacattt accgggatcg ttctactccc
    15421 gccgagacgc tcaatctccc gttcctgcaa tacgcgcagc agtttaggct gaagttctaa
    15481 cggcagatcg ccaatttcat cgagaaacaa cgtgccgcca tcggcaattt caaaacgacc
    15541 acgatgggta ttaatcgcac cagtaaacgc ccctttgtca tgaccgaata actcactttc
    15601 cagaagactg gcggggatgg cagcgcagtt gattttgacc agcggcttgt cgcgtcgcgg
    15661 gctaagttga tggatcgctc tggcgatcac ctctttgccg gttccggttt caccgcaaat
    15721 caacaccgta ctgtcgctct tcgccacaat atctacctgc tggagcaggt cttccattgc
    15781 ctggctttga tagataatgt caccgacgcc cagattcgaa aggagctgct cgctaagctg
    15841 gtggttttct tgctgcaaac tttcctgcaa atcggtcatt ctacgccagg catcggcatt
    15901 gccaacggca atagcgatgc gatcggctat gtgttgtagt agctggcagt tttcctcact
    15961 aaagagagtg gaagaggtat gcgccagcaa caatgcaccc ggtgtatggt tgccaaaggt
    16021 aagcggtata aggagcgcag attcgcagcc gttagatgca agtaagagta ataacgggtc
    16081 gcgttgccag agaaacagat cgtctgcacg gtgcgtcaag gtcggttgtc gggtttgtag
    16141 cgtttgtgtc agcaatacac tgtcgccagg catattgcgt ggcagacacg cacaatgtga
    16201 ggcagaaaga tcgctgcacc acaggctaaa cttctcgttc tttcgatgat cgcccagtac
    16261 catactgact gaagccagac cgaaaaaatg atggatctca cgagcgacgt cagcgatcag
    16321 atcgtcgaga tcaagatgtg acagcactgc attagtaata tcaaccagta cccgatgatg
    16381 atcgcgatct ttctccagca aacgcagtgc cacctgctgt tcttcatcat tgagccatga
    16441 ttgcagaacc ggggtgatta tccgcgccag ccagttaaac agtgtgcaat cctggggagt
    16501 aaaacgactg ccgtgggtgc gaataaagcg tagctccgcc attccactac tgccgagaag
    16561 gacggcgtga cgcgaataat cgaccatctc gccctgatac tctgttgttg ccgtcacatc
    16621 gctgccatca tcatggtagc ggtaatagaa gttgcttttg agaacatgca gttcaataag
    16681 ttgtaccggg ctgaacggcg gcggcagcgg gatgaaggcg cgtaataaag acgccttacc
    16741 gtgtttctgc gctaaccgct ccgcgagcat tccgtttacc gcttcaattg ttattccttg
    16801 cggcggggca aacatcgcct cgtctgacat agccatcaac agtccttatt aatgagaaat
    16861 ccttctcacg taatttttct ggccatcaga tagatggccg gttcctgcac aatttcatcc
    16921 aacagatgaa tgaattgcat gctacgcgcc tgccacacag acgttgcgtc atctgccagt
    16981 ggcgcgaatg cccgggtcaa cagggcggtg tggctggcgt caataacaat ctcaccaaaa
    17041 gtcatcaccc ccagcatctt acgtcgggct tgctcctctt cgacctgggc aagccaatct
    17101 tcgtattccg tcagtgggca gcggaaggcg acattcagac aatcaatcac gccaacgtga
    17161 tggccgatag ccagcgagta atacattacc tgagagctgt gttccggcat ctcgtcgcta
    17221 ctggcgacaa acttttttcg cagcgtccag aaaacaattt ccccgcactc ttcagtcatg
    17281 taacccccgg agtacgcgaa agagttgctg aacgatacta ttgagacgtg gatcgtcggc
    17341 atcgcgcaac caggtgttaa cccgatttcc tgtaggatcg tcgcttaaat ggcgcaggag
    17401 ccgatcgcaa atttctcgcc cctgacgata gcccgccagc cgccgcgctt ctcgctcaat
    17461 ggcgatacgc tgtgatggcg ggatctgtgg ccacaacggt tgcatagtca ccccggtggg
    17521 atcgcgataa tccacagcgt gaatcttctg ttgcagcaaa ccgagcgcca cggcgaaacc
    17581 gtgaatggtg gcggccggtg ttggcgggca gccggggatc caaacatcaa tggggacaat
    17641 ggtgtcgctg ccgccccaga cgctgtagag atcgtggaaa ataccgccgc cgacaccgca
    17701 cgcgccgtag gaaacacaga ttttatgatc gggggcagac tcatacgccc gaagtgcagg
    17761 catacgcatc gcccgggtga ctgcgccagt aaataacaaa atatcggcgt gacgcggtga
    17821 tgaaacaacc ttaatgccaa aacgttctgc gtcgaatacg ggggtgatgg cagcaaaaat
    17881 ttcgatttca caggcgttgc agccgccgca atcgacgcga taaacgtagg ctgagcgacg
    17941 gatatcctgt agcaaatgcc gcttcatctt ttgcgtttgc tcatccagcg tgatgggctg
    18001 gctgacatgt tgtgtaagca ctggactcat agctgctcct tagccaccag cagtacatct
    18061 gtatcgtcgt tgatcagcgt cgcgcgttgt ttgcactccg ggcagacgct cgcttgcgcc
    18121 cgcaacattt cgcggttttg tggcgcattt tgttgctgtg ccaacaattc agcagccagt
    18181 gcgacggttt tttgcggggc aaacgggcgt tcgcaacggc tgcaacgttg tagatggaac
    18241 gtcgcgcggg tatagagatc ggctttattg gtgacggtca gttcaaagtt attggtaagc
    18301 tggatggctc tggtcgggca cacttcttca caacgtccgc agtaaataca acgccccaga
    18361 tagagctgcc aggtgcgcga attttgctgg tcgtcggtct ggatagtcag cgcatttgcc
    18421 ggacaagcac aggcgccgca ggcaatacat tggctgggca tcaggtccgg tttcccgcga
    18481 aagcctgggc tgacctccag tggcgcgaag ggatatttca ccgtcgcggt tccggcgcgc
    18541 ataatagttt tcagtaactt cagcatcttc tgtccttact tcagcggcga acggttacga
    18601 tcaatgccgt agcgttcgat ctctttatac ggcacggttt ttgactggcg cttgcgcaca
    18661 tctaccagcg tcacacggtc ggtacaggag tagcagggat caaggctacc gataatcagc
    18721 ggtgcgtcag aaacggtatt gccgcgcagc atgtaacgca acaccggcca gttggcgtag
    18781 gtggcggcac ggcagcgcca gcggaacaat ttttggttat caccgagcat gctccagtgc
    18841 acgtcttcac cgcgtggcgc ttcaacaaag cccagcgcga atgcgtgagg tttatagcta
    18901 aagccttcgg tcagcagtgg ggtatccggc atgttgtcga gagcaaattc cagcattgcc
    18961 agcgaatcaa acgtctcttt gacacggacc gtcacgcggg agaaaacatc gccgccggta
    19021 aaggtaaaca gtgtttttgg aatattgccg tagtcggcgt aggggtgatc aaagcgcaaa
    19081 tcacgggcaa aaccactgcc gcgaatcagc ggccctacag ggctataatc acgggcgatt
    19141 tgtcggtcga gaatgccaat gccctgagtg cgttgttcca tattcggcgt agcaagcagc
    19201 atctctacca gcaccgacac gtcggcgcgc atctcgcgca ccagtttcag cgtttgcaga
    19261 cgttgctctt tgagaatatc gcggcgaaca ccaccaatca gattcagacc gtaggtttta
    19321 cgcgacccga tcagcaattc cgccatcgtc atcgactttt cccgcacgcg gaaaaattgc
    19381 ataaagccgg tatcaaaacc aacgaaatgg caggagaggc caaggttaag caaatgactg
    19441 tgtagccgtt cgacttccag cagaatcgag cgaatagtat gtgctcgttg cggcacctca
    19501 atccccagtg cattttcaac cgaattggta taggccacac tgtgggcaaa accgcaaatc
    19561 ccacacacgc ggtctgataa gaaggtcact tcgttgtagc ccatccgcgt ttctgccagt
    19621 ttctccatgc cgcgatggac ataaaacagg cggtaatcag catcgacaat ttgctcgcca
    19681 tccacaaaca agcggaagtg acccagttca tcggaggtga tatgcagcgg gccgacaggg
    19741 atcacctgcg catcgctgtt gccctcattg ataaacggat acgtttcgga atcagtcgtc
    19801 ggttcagggc gcagtcgata atccatcgca tctttgcgca gcggatgcat atcttccggc
    19861 cagtcatcgg gcaacaccag gcgacgctga tccggcaggc caaccggaat cagcccgtac
    19921 atatcgcgaa tttctcgctc gccccagacc gcggcaggga cgcgcggtgt gactgacgga
    19981 aactcccgac tgtcagcatc gaccagtgcc ttcactacga tccagcattt ttcggcccct
    20041 tccatagaaa gggcataata aaccgcgtaa tgaccgttaa gtgtccgctc gtcgttgcca
    20101 aacagaaccg gaagccagcc atcatgttga taataaagat aatgtacaac gtcaggcagc
    20161 agattgattt tcaccgtaat ggtgacctgt tcaggcgttt gtcgctcttc atccagcacc
    20221 gcgccgggga actgcgtttt cagggcggcg agaatcgctt cgccacgatt tgacgatgaa
    20281 ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct gaaggcaaaa aatcatgcca
    20341 ggtgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg actatagtgg aagcgcccgc
    20401 caggatcctg atgacaggtt gtggaatatg cgttcccatc gccagcatca tgaccagcag
    20461 aatcaccatt ggcgaggtgg tcaaccagcc gagatcaccc cggttaacgg cctgcggcgg
    20521 tttcgccatt aacacccgcg cagccatccg taccaggccc gccagcacca gcgttaacag
    20581 caataacagc aggacgataa tcagcaggtg attacgtgcc agtccggcgg taacggtcat
    20641 aaattcgcta agaaaaatgt tgaagggcgg catccctgcc agcgccagcg caccgccgcc
    20701 aaacagcacg gcggtaaatg gcatgatttt gagcatccct cagacgacgt tgagatcgcg
    20761 cgtgccgtac ttgagcagta cattgccgga accgcagaac agcagcgttt ttgccagact
    20821 gtggtttaag gtgtgcagca gcgcggcaaa aattcccagt ggcccgccaa tgcctagcgc
    20881 caccgcgacc agtcccatgt tctccacgct ggagtacgcc agcagacgct taatgtcccg
    20941 ctgtaccaga atgaaaaatg ccgccacggc aaccgacaac atgccgaaga tgagcaacaa
    21001 ccggttgggg aaatcgctgc cgatggcctg gcagataatg atgtaatagc gaattagcac
    21061 caacagcgcg cagttcagca atacggcgga gagcagggcg ctgaccggac tcggcgcttc
    21121 actgtgagca tccggcagcc aggcgtgcat gggaaatagc ccggttttgg tgccaaagcc
    21181 aattagcaca aacacaaagg ccaacagcat taatgttggg tcaagcaagg acgattgctt
    21241 aagaacctcg ctccagaata tcgccatttc tgcctgcggc ataacgctgg cgacgttggc
    21301 gtataccagc acggtaccga acagaccaaa agcgacacca acagtacaaa taatgatgta
    21361 cttccatgct gcttccagcg atgaacgctg accgtaaatg cctaccagaa acgccgagct
    21421 taaggtggtg gcttcgatcg ccgcccacat cacaatcagg ttattgctgg taacaaccag
    21481 cagcatggtg aacaaaaaca gatggaagaa accgtagtaa tcgcacagcg taacgggtga
    21541 aagctcgccg tgtgccactt catgacgcat gtagccaatc gagtaaacac cggtgagaaa
    21601 gccaatcaca ccaagaatgg cgaggaacaa accgcccaga ccatcaatat gaagccacag
    21661 tcccgcagcg aatatttctc ctgcatcagc ggcagtttgg accacccaga gtgccagaat
    21721 cagcagcagt gtgatcccta agctatgtaa tacagtcacc gtacaagtcg cagagagccc
    21781 ccgtttccgg caggcaaaac agagcagcga aaaaagcagc ggcgtgagca ggagtaaagc
    21841 gaacatcaca gaataactca tctcattatc ccttcagcgc ggtcaagttg ttcacgtcca
    21901 gcgtgccgtg ggtacgccat atttttcttg ccagtaacac catcacaatg acggcgaaga
    21961 tggcgtcggt agcgatacct atttccacca gttccggtgc tcgccaggca agaagcgcca
    22021 gcaccagatg ggagccgttt tccatcaggc agtaaccaaa aatttgccgc aggatattgc
    22081 gctggctgac aatgcacagc aggccaagca gaaaatgacc taacgctacc gccagcgccg
    22141 gtttcagccc ggtagccatc ggtagcttca cgggctgaac gacaaatgcg caaagcagga
    22201 caattaacgc cgcgagcagt gccatcattg ccggaccgaa taacgctttt tccgggatgt
    22261 tctggggaat atttcgtgca gcgtaagtca tgattaacgg taccagcagc actttggtga
    22321 taaaggcgct ggcggaccag atcagcagtt gctctgcggc gaacaggcac gagagagtgg
    22381 cgaaaataga caccagcacc agcgactggc aggcgtaaaa tccgcatgac aggcgatagc
    22441 ttttgacgct aatcacaaac agcgatgtca gcatcatcag tcccgccaga ttatttacga
    22501 tcatagaacc ggtcattcat catctcctta ctgcaaccag gtcgcggcga ttacgctgga
    22561 aatcagcgac atcacaatca gtacaatcaa caccaggcgc attgaccctg gcagcggtgc
    22621 ggcatcggcg acggcctcgc tcggtttgcc aggcacaacg cgaccaaacc agcgaataaa
    22681 ccaggcgaaa ctggcgaccg attcaatcat cagcagaatc atggcgggca gcaggatcca
    22741 gtactccact gacaacgcaa aaccggcggc aaacagcggg aatttactga agaagccgtt
    22801 gaacggcggc acaccggtaa tcgccagcgc tgccacgcag aaacccacgc ctggcaacgg
    22861 cagggtgtgc aatacgccac gcagacgcgg caacaagcgc gtgccgcagc tgtaactcag
    22921 cgcacctgct acaaggaaaa acaggctttt agcgaacgcg tggttgacga tgtaggcgat
    22981 gctaccctcc agcgccagcc gcgagccgaa gatggagagc gacaagccga agaacatcca
    23041 gccaagttga gtgatggtcg accaggccag caaccgcttc atatcctgct gtggcaaata
    23101 catcagaaag ccataaagaa tggtgaccag tgccatgacc atgccaacgc cgccaatcac
    23161 atgcgggata ttgccgccgt cgataatggc gcgggcaaaa atgtaaacgc ccactttcac
    23221 catcgatgcg gcgtggagat aggcgctgat cggtgttggc gcttccattg cgtccggtag
    23281 ccacgcttgc atcggtagct gggccgattt cccccacgcg gcaaacagga tgccgccata
    23341 aaccagataa cgtgcgtcgc cgtgtaactc gctcatcgcg ctaagcgcaa acgttccggt
    23401 ctgcaaaaac agcgtggcgg cggcaagata caaccccaac gaaccgatat gggtgataag
    23461 caacgctttt agtgctgaac gctgcgcttt atcgctctgg taataactga tcaacgccca
    23521 ggagcagccg ccctaatttc aaaaaacaac aactgaccga gcagcgtcga ggagagtacc
    23581 agtcccgcca tcgcgccgat aaacaccagc aaaaatgcgt aataacgatt cgtgccgtta
    23641 tgcgggtgtt cgcgattttt atccgtcaga taacccgtgg agtagatcgt gaccagcaaa
    23701 ccgagaaaca ccaccacaaa cagaatcagc gtactcacgc gatcaatgac caggccaaac
    23761 aatgccacat cccccacgtt gaccaacgtc aacgtgacgg caactttatc ggcctgataa
    23821 aacgcggaga tcagcgacaa catgcacagc gtggtcagtg cggcgaacaa aacccccatt
    23881 cggcggcccg acgttgtggc gaaaacgaaa cgaccagtgc gccgataaaa ggcagcatta
    23941 acgtcgtcag agcaagattt tccatattcc gtcagtgctc cttacagacc ggttaaccag
    24001 aagacccatg caagcgcagc aaggctgaag ccaagccagg tcacatggtg aatgagtaaa
    24061 aaacgcccgc gtgccagcgt gttttctgcg attgaggcca gtacaaaaat cagcaaaacc
    24121 ttgagcagcg taacgacaag tgaagtcagc aggcaggcga gagaaagttc ttgcgcgcgc
    24181 ccaaagggca gaaacagggc cacaaacagt gatgccatca cgacctgttt cagccccagc
    24241 ccccatttcg ccagcgccag cccggcaccg gaatattcgg tcagcgggcc ttcctgtaat
    24301 tcctgttctg cttcagcaac atcaaaggga atttttccca tctcaatgaa gcaggcaaaa
    24361 ccacaggcca gtaacgccag tacggtggtt agcggcgagt tccagcccgt tgccagcgta
    24421 ttgctgatca tctcgatatg cgtggaacct gctatcagcg ccaataccag cagtgagaga
    24481 atgagcatgg gttcgaccag aatgccgagc gtcaactcgc gactggcacc gactcccgca
    24541 aacgggcttc cggtatccag cccggaaaga gcaaagaaaa aacgaaacag ggcaagaaga
    24601 tagataaggg tgatcagatc gccgccgccc gcaaaagggg aaacggtaat aaaaagtggt
    24661 agggccatcg ccagcaccag catgctgctg attaataccc acggcatcag gcggaacatc
    24721 agacctgaag atataggcgc aacttcctgg tgtttaaaca gtttgtggat atcgcgataa
    24781 tcctgccaga tccccggccc gcggcgggag tgcatacgcg cgcgtatctg ccgggaaata
    24841 cccgtaaaaa gcggggttag cagcagcaga atcacggcct gtgctaacgc aaaaatgacc
    24901 agatatccgt cgcaaagagt ttgtctcatg gtgatttcct tagacggcaa tagcgattag
    24961 cagcacaacc agtgcggcga ccacatacag gcaatagaga cgaaagtcgc cgctttgcag
    25021 atgctgtatt tctttggcca gccgttgggt ggcgctcacg atggggcgga tcacccgctc
    25081 atcccagaag ggttctgtgc tctgagccct ggcggtgacg tgcgcaagac ctttatttag
    25141 cctcagcgta gggtcgagct gttgtcgtag acgaaatagc gcagaaaaga ccacacgcag
    25201 cggctgcatc acgccattgc ctgacggggc catcgcattt tcccagccgt agccgcatgc
    25261 ctaggcatct cctgtgcgac gaaacgctgc gcggcgcgaa cgacaccaca gccagtaaag
    25321 gccaggcagc aaaggcatcg ccagtagtaa cagcaacagt aatgagggtg tgacccgtgt
    25381 atgaaacgtg ccgggtacaa gtgctattcc gctggcgaca gtgacgggag gggtattggt
    25441 aaacgcatgg gcaatgtgca ttatcttcgg tgccagccaa ctggcactaa gcgcaattaa
    25501 tacgcagagt gccgcgagca gtagcatggc gacgatcatc gtatttggca cttcctgtgc
    25561 ctcttcagcg tgtgtgctgc gcggcgcacc acagaaagta ataccgtaca ttttgacgaa
    25621 gcacattact gccagcccac cagtgactgc cagcattacc atagcaatag gacccgcaag
    25681 ttgtagcgct acggcttcca cacgacttag tgagaacagc gactgccagg tgtaccattc
    25741 gctgataaaa ccattcagcg gaggaatggc tgatatcgcg aggcaaccaa tcaggcatgc
    25801 tgcggctgtc cacggcatcc gtttcgccag tgcccccatt ttttccatgt cgtgggtatg
    25861 caaacgcgaa ataatcgcgc ccgcgccgag aaatagcagc ctttgaacag cgcatggttg
    25921 agcagatgaa acagtgcgcc gagcagtcca accacggtga gcagcgggtc gtgcagtgac
    25981 agaccgacca tcgccacacc gactgccagc aaaataatgc cgacgttttc gacggtactc
    26041 caggccagca gccgtttgat atcctgttcc gccagcgcat atagcacgcc caggagcgcg
    26101 gagattgcgc cgatcgccat caccagaatg ccccaccaca gaggcaaacc cgtttgcgcc
    26161 agcagatcta tcgctacttt caggatgccg aaaataccga ttttgaccat tacgccagac
    26221 atcaacgccg aagcgtgcga tggtgcggca gggtgagcgc gcggcaacca gctgtgcaac
    26281 ggcatcatcc cggctttcgc gccaaaaccg aaaaaggcca gcagaaacac cgccgacgcc
    26341 agccccggag aaagtgaaag cgtgcgaaac tggcaaaatc gaggctgccg ctttcgcgcc
    26401 ccatcagcaa gaaggcgatc ataatcagca ccgatccggc gtgggcgata aaaaagtaga
    26461 gcatgccagc gttgatcgac gttttatcct gcctggcaat gaccagaaac caggaagaca
    26521 gcgacatcat ttcaaacagc acgatgaacc aaaaagcgtt gtccatcacc agcagggcaa
    26581 ccatcgatgc gatgaaaaga ttcataaaga agcccatcgc cgccgcgcct ttgccctcgt
    26641 attcgcgcat ataagtcaat gaatagagcg aacaaaccac caccagcaag gagataacca
    26701 gcaccataaa tgccgagagg ctatccattc gcagggttaa atcggcaaag tcaaaagggg
    26761 tggcaaaatg ggcgactaaa gttacaccag catgtaattg cgtaattccg ctgatgatgc
    26821 caatcacgcc gcccaccagc gatgtgatgc cggaaagctt aatagccagt ctgtccagac
    26881 cgagtaaaaa cagcgaagcc agactggcaa acagatagag gatcagcgac caggttaata
    26941 attgcagggc atccattagt gttgctcctg agtgagggaa aggggatcct ccccgccatt
    27001 ggcaaaccag cttgcggcaa ggcgctgttt ttctttcatc tgacgttgca ggctatcgtc
    27061 ggtgatcagc cgtaacgcct gattcgggca agcgcgaacg caggccggac cttctggcaa
    27121 gaaatcacac aggtcgcatt tcaccgcgac ggtctggaca cccggttccc agcgcagcaa
    27181 cgcatgttgg ggcggcacgt tttcttcgcc gtctttgagt gagccttcag cctgaaaaac
    27241 atattgcgca tgggcattca ccggacggct tcctgaagcg ctgattgcgc caaatgggca
    27301 gaccacggcg caaagcttgc agccaataca gaggctttcg ttgagttgga tcgcatcatc
    27361 cctctgagag atggcattga ccgggcagac ctgcaggcaa ggggcttcct cacagtgatg
    27421 acacacgaca ggggcagtga ttgttgacgt cttcgccagg gccaggcgcg ggtgttgctg
    27481 taaaccttgc gttttatgca cgtccgaaca ggcagcgaga caggtattac atcctgtaca
    27541 ccacagtggt tcggccacca caaagcggtt catgtcggct ccttgaaaat gatatgaaac
    27601 attgcatttc atattgcata aaggatacca ttctgattaa cccaatatat tcaataagtt
    27661 atgtattaac tggattctga acggtacaaa aatgatggtc attaacgttc agcaaaaaat
    27721 ggattcgaca cccgtgtcgc aggggaaagc gagaatgtga tcttgatcac gagaagtgag
    27781 gtatgctggc cgcaagcgca gccagcacgg aatggagcaa agtaatcagg cgtgttcttt
    27841 cagccagttc agcacaacgt cgtggtgatt gctggttttg aaatcgtcaa agacatgttc
    27901 gattttgcca tcagcgtcaa tcaggaagct gatgcgatga ataccatcgt aggttttgcc
    27961 catgaaggac ttttcacccc agacgccgaa ttgttcgcac acctggtggt cctcatcaga
    28021 caggagcgta aagttaagca gctctttttc cgcaaaacgg gagagttttt cgggtttatc
    28081 ggtgctgata cccagcacat caacgcccgc ttttttcaaa tcatccatgt tatcgcgtaa
    28141 gccgcaggcc tgtacggtac agccgggggt catggctttc gggtagaaat aaaccagaac
    28201 acgctgtccc tggaagtcgg tcaaattaac ttgttctccg tcttgatccg gcaagctaaa
    28261 tttcggtgcg atatcaccgg ctttcagtgg attcattact taactccatc ctgttcatca
    28321 tgttgggaat aattgacgac gttaatactg ccttgtgcat tgagttctgt acatagggct
    28381 ttgaacgctt gctcaatatt tgctgcgtcc gcagatgcgg ggctgtgggc ggttatctga
    28441 atatgcaact gcgcagcccg ttcattttca gcaggttgcg tgcgcgacac cagctccgca
    28501 atgttcatat gatgcgcgtc gaaaagtgct gtgaagcgtt caattaaatg cggggagtct
    28561 gccacatcga cttgaaccca gacagatgct ggcattggcg gacgcggacg cgccgtcgtg
    28621 cgcttcatca cgattaaaag atccagttcg gcacctttca acggtaacgt tgattcaatc
    28681 agagtgatgg cattccatga accggaaagc agcatgataa acgtgaactc ttctcccagc
    28741 atcgccaggc gactgtcttc aatattacag ccgcaactac tgacatgacg ggtgatggtg
    28801 ttcacaattc cagggcgatc ggcacccaac gcagtgatca ccagataatg ttgcgatgac
    28861 agtgtcaaac tggttattcc tttaaggggt gagttgttct taaggaaagc ataaaaaaaa
    28921 catgcataca acaatcagaa cggttctgtc tgcttgcttt taatgccata ccaaacgtac
    28981 cattgagaca cttgtttgca cagaggatgg cccatgttca cgggaagtat tgtcgcgatt
    29041 gttactccga tggatgaaaa aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat
    29101 tatcatgtcg ccagcggtac ttcggcgatc gtttctgttg gcaccactgg cgagtccgct
    29161 accttaaatc atgacgaaca tgctgatgtg gtgatgatga cgctggagct ggctgatggg
    29221 cgcattccgg taattgccgg gaccggcgct aacgctactg cggaagccat tagcctgacg
    29281 cagcgcttca atgacagtgg tatcgtcggc tgcctgacgg taacccctta ctacaatcgt
    29341 ccgtcgcaag aaggtttgta tcagcatttc aaagccatcg ctgagcatac tgacctgccg
    29401 caaattctgt ataatgtgcc gtcccgtact ggctgcgatc tgctcccgga aacggtgggc
    29461 cgtctggcga aagtaaaaaa tattatcgga atcaaagagg caacagggaa cttaacgcgt
    29521 gtaaaccaga tcaaagagct ggtttcagat gattttgttc tgctgagcgg cgatgatgcg
    29581 agcgcgctgg acttcatgca attaggcggt catggggtta tttccgttac ggctaacgtc
    29641 gcagcgcgtg atatggccca gatgtgcaaa ctggcagcag aagggcattt tgccgaggca
    29701 cgcgttatta atcagcgtct gatgccatta cacaacaaac tatttgtcga acccaatcca
    29761 atcccggtga aatgggcatg taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg
    29821 ccaatgacac caatcaccga cagtggtcgt gagacggtca gagcggcgct taagcatgcc
    29881 ggtttgctgt aaagtttagg gagatttgat ggcttactct gttcaaaagt cgcgcctggc
    29941 aaaggttgcg ggtgtttcgc ttgttttatt actcgctgcc tgtagttctg actcacgcta
    30001 taagcgtcag gtcagtggtg atgaagccta cctggaagcg gcaccgcttg cggagcttca
    30061 tgccccggct ggaatgattt tgccggtgac ctccggtgat tatgcaatcc cggtgaccaa
    30121 cggtagtggt gctgtcggta aggcgctgga cattcgtcca ccagcccagc cgctggcact
    30181 ggtttctggc gcgcgtaccc agttcacggg cgataccgct tcattgctgg tggaaaatgg
    30241 tcgtggcaat actctgtggc cgcaggtggt tagcgtgctg caggcgaaaa actacaccat
    30301 cacccaacgt gatgatgctg gtcagacact gaccaccgat tgggtacaat ggaaccgtct
    30361 ggacgaagac gagcagtatc gtggtcgtta tcaaatctct gttaagccgc agggttatca
    30421 gcaggcggtt acggttaaac tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc
    30481 ggcttccatg cagcgttaca acacggagat gatgaacgtt atttccgccg gtctggataa
    30541 atctgccact gacgccgcga acgctgcgca aaatcgtgcc tccaccacta tggacgtaca
    30601 aagtgcagct gatgacaccg gtttaccgat gctggtcgta cgcgggccgt tcaatgtggt
    30661 ttggcaacgt ctgccagcgg cgctggaaaa agtgggcatg aaagtgaccg acagcacccg
    30721 ttcgcagggt aacatggccg taacttataa gccgctgtct gacagcgact ggcaggaact
    30781 gggcgcgagc gatccaggcc tggcatccgg tgactataaa ctgcaggttg gcgatttaga
    30841 taaccgcagc agcctacagt tcatcgatcc gaaaggtcat actctgactc agagtcagaa
    30901 cgacgcgctg gtatctgtct tccaggctgc gtttagcaag taaaaataca gggctggaat
    30961 catccggccc ttttttctga tatgatacgc aaacgtgtgc gtaggcagga aaacgcgatt
    31021 ttagcggtaa ttcgcacgaa atttgtttgt cggacgtagt tcgaataagg cgttcacgcc
    31081 gcatccgaca aaacatccgg cacaccagac agcaaaagat tttaaaacgt taattcatac
    31141 ccaggagtga taaagatgca aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta
    31201 tacagcacgg aaaacccgga cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg
    31261 gatggcgcgc gcattgagca gtttgatcgc aaaggtatgg tgaacaacaa gttcaactac
    31321 ttcatcatga gcaaactggc tgaagcgggt atcccgactc aaatggagcg tctgctctcc
    31381 gataccgaat gtctggtgaa aaagctggat atggtgccgg ttgagtgtgt cgtgcgtaac
    31441 cgtgctgctg gctctctggt gaaacgtctt ggaatcgaag aaggtattga gctgaacccg
    31501 ccgctgttcg atctgttcct gaaaaacgac gccatgcacg atccgatggt caacgaatct
    31561 tactgcgaaa cctttggctg ggtgagcaaa gagaacctgg cgcgtatgaa agagctgacc
    31621 tacaaagcga acgacgtgct gaaaaaactg ttcgatgatg ctggtctgat tctggtcgac
    31681 ttcaagctgg agtttggcct gtacaaaggt gaagtggtac tgggcgatga gttctccccg
    31741 gacggcagcc gcctgtggga caaagaaaca ctggagaaaa tggacaaaga ccgtttccgc
    31801 cagagcctcg gtggcctgat cgaagcctat gaagccgtcg cccgccgcct gggtgtacag
    31861 ctggactgat ttttctggtt gcttgtttct ttagccggag ggtactccgg ctaaatgtct
    31921 ttgatacccc cgctacaata cttctcatta tctccctttg acaggaaagt cataccaggc
    31981 caatatgtat atacataacg tatatcccaa aaaagggggg gctatggaaa ctactgtatt
    32041 tttaagtaat cgcagtcagg ccgtcagatt accgaaagcg gtcgctctgc cagaagacgt
    32101 taagaaagta gaaattattg cgattggtcg tacaagaatc atcactcccg ccggtgagtc
    32161 ctgggacagt tggtttgatg gcgaaaatgt gagtgcggat tttatggata tccgtgatca
    32221 acccgcaatg caggaaaggg aatcattttg atgctgaaat tcatgctgga taccaatatc
    32281 tgtattttca ctattaagaa taaaccagta catgtcagag agcggtttaa cctgaattct
    32341 ggccggatgt gtataagttc tgtaacattg atggagctta tctatggggc agaaaaaagt
    32401 cagatgccag aacgcaattt ggcggttatt gaaggtttcg tatctcgttt ggtagtgctt
    32461 gattatgata gtgctgctgc gacgcatacc gggcaaatca gggccgagct tgcccggcag
    32521 ggaaggcctg ttggaccttt tgaccagatg atagcgggac atgctcgcag tcgcggtttg
    32581 attgtggtta ccaacaatac ccgagagttt gagcgagtgg cgggaatcag actagaagac
    32641 tggagctaac attctctttt acttctaaca ttcgtaaatt atataacgtg gcatgaagcc
    32701 ttaattcctc cgccatttca tcccgctttc ctgtggtaat cttcctctga accacctacg
    32761 atcatttcta taatgaatat attgttgagg tatctatgcg ttggcaaggg cgacgtgaaa
    32821 gtgacaatgt tgaagacagg cgcaacagct ccggcggtcc gtcaatgggc ggtcccggtt
    32881 tccgtctgcc gagcggtaaa ggcgggctga ttttactgat agtcgtgctg gttgcaggct
    32941 actatggcgt tgatttaacc gggttgatga ccgggcagcc ggtttctcaa caacaatcaa
    33001 cgcggtcaat tagccctaat gaagacgaag ccgcaaaatt cacctcggtg attctggcaa
    33061 ccacggaaga cacctgggga caacagttcg aaaagatggg taagacctat cagcaaccga
    33121 agctggtcat gtaccgtgga atgacgcgca ccggctgcgg ggcgggccag tccattatgg
    33181 ggccgttcta ttgcccggcg gatggcacag tttatatcga tctctccttc tatgatgaca
    33241 tgaaagacaa acttggcgcg gatggcgatt ttgcccaggg gtacgttatc gcccatgaag
    33301 tcggtcatca tgtgcagaaa ctgttaggca tcgagccgaa agttcgtcaa ctgcaacaaa
    33361 acgcgacgca ggcggaagtg aaccgcttat ctgtgcgtat ggaactccag gccgactgtt
    33421 ttgccggtgt ctgggggcat agtatgcagc agcaaggcgt tctggaaacc ggcgatctgg
    33481 aagaggcgct gaacgcggcg caggccatcg gcgatgaccg tttacaacag caaagtcagg
    33541 ggcgagtagt accagatagt ttcacacatg gcacttctca gcaacgctac agctggttta
    33601 aacgtggttt cgacagcggc gatccggcac aatgcaatac ttttggtaaa agcatttaac
    33661 ttccggggca gggatggctg aactgactgc gcttcacaca ttaacagcgc aaatgaaacg
    33721 tgaagggatc cgccgcttgc tggtgttgag cggggaagag ggttggtgtt ttgatcatgc
    33781 gcttaagttg cgtgatgcct tacctggcga ctggctgtgg atttcgccgc agccagatgc
    33841 tgaaaaccac tgttcaccct cggcgctaca aactttactt gggcgcgagt tccggcatgc
    33901 ggtattcgac gcccgccacg gctttgatgc cgctgccttt gcagcactta gcggaacgtt
    33961 gaaagcggga agctggctgg ttttgttact ccctgtatgg gaagagtggg aaaaccaacc
    34021 tgatgccgac tcgctgcgct ggagtgattg ccctgaccct attgcgacgc cgcattttgt
    34081 ccagcatctc aaacgcgtac ttacggcgga taacgacgct atcctctggc ggcaaaacca
    34141 gccgttctcg ttggcgcatt ttactccccg tactgactgg caccccgcga ccggcgcacc
    34201 acaaccagaa caacagcaac tcttacagca gctactgacc atgcctccgg gcgtggcagc
    34261 ggtaacggct gcgcgtgggc gcggtaagtc ggcgttggca gggcaactca tttctcgtat
    34321 tgcgggcagt gcgattgtca ccgcgcccgc aaaagcggca acggatgtac tggcacaatt
    34381 tgcgggcgag aagtttcgct ttattgcacc ggatgccttg ttagccagcg atgagcaagc
    34441 cgactggctg gtggtcgacg aagccgcagc catacctgcg ccgttgttgc atcaactggt
    34501 atcgcgtttt cctcgaacgt tgttaaccac tacggtgcag ggctacgaag gcaccggacg
    34561 tggttttttg ctgaaatttt gcgctcgctt tccgcattta caccgttttg aactgcaaca
    34621 gccgatccgc tgggcacagg gatgcccgct ggaaaaaatg gttagtgagg cactggtttt
    34681 tgacgatgaa aacttcaccc atacaccaca aggcaatatc gtcatttccg catttgaaca
    34741 aacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc
    34801 gcactaccgg acttcgccgc tggatttacg ccggatgatg gatgcaccag ggcaatattt
    34861 tttacaggcg gctggcgaaa acgagattgc cggagcgctg tggctggtgg atgagggggg
    34921 attatctcaa caactcagtc aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct
    34981 ggtggcccag tcgctggcgg cgcacggtag caatccactg gcagcgacat tgcgtggacg
    35041 gcgggtcagc cggatagcag ttcatccggc gcgtcagcgg gaaggcacag ggcagcaact
    35101 tattgctggt gctttgcaat atacgcgtga cctcgactat ctttcggtga gttttggtta
    35161 caccggggag ttatggcgtt tctggcaccg ctgcggtttt gtgctggtgc ggatgggtaa
    35221 tcatcgtgaa gccagcagcg gttgctatac ggcgatggcg ctgttaccga tgagtaatgc
    35281 gggtaaacaa ctggctgaac gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc
    35341 aaagtggaat ggcgaaacgc ttcctgttga tccactaaac gatgccgtcc tttctgacga
    35401 cgactggctt gaactggccg gttttgcttt cgctcatcgt ccgctattaa catcgttagg
    35461 ttgcttattg cgtctgctac aaaccagtga actggcatta ccggcgctgc gtgggcgttt
    35521 acagaaaaac gtcagcgacg cgcagttatg taccacactt aaactttcag gccgcaagat
    35581 gttactggtc cgtcagcggg aagaggccgc gcaggcgctg ttcgcactta atgatgttcg
    35641 cactgagcgt ctgcgcgatc gcataacgca atggcaattt tttcactgac tccttcaatt
    35701 ttctcccatg gtcattgtga gtcagcggcg taaaattaat gacatcagtt aaggagttcg
    35761 ccatgaaaca tgaccatttt gttgttcaaa gcccggataa acctgcgcaa cagttgttac
    35821 tgcttttcca cggtgtcggg gataacccgg tggcaatggg ggaaataggt agctggtttg
    35881 caccgctgtt tcctgatgcg ctggtggtga gtgtcggtgg cgcggagccg agcggtaatc
    35941 cggcggggcg tcagtggttt tcggtgcagg gtattacgga agataatcgc caggcgcgtg
    36001 tggatgcgat catgccgacg tttattgaaa cggtgcgcta ctggcagaaa cagagcggag
    36061 taggggctaa cgccacggcg ctcatcggtt tctctcaagg cgcgattatg gcgctggaga
    36121 gcattaaagc cgaaccgggc cttgcttcac gcgtcattgc ttttaacgga cgctatgcga
    36181 gcctgccgga aaccgcgtct acagccacga caattcactt gattcatggt ggtgaagatc
    36241 cggttatcga tctggcgcat gcagttgcgg cgcaggaagc gttaatcagt gccggtggtg
    36301 acgtgacgct ggatatcgtg gaggatttag gccatgcgat cgacaatcgc agtatgcaat
    36361 ttgccctgga tcacttgcgt tataccattc cgaaacatta tttcgacgaa gcgctaagtg
    36421 gcggtaagcc tggcgatgat gatgtgattg aaatgatgta aaagacagat tacctggcct
    36481 gataagacgc gt
//
LOCUS       DAJSKJ010000019        34951 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000019 DAJSKJ010000000
VERSION     DAJSKJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.019"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            109..576
                     /gene="slyB"
                     /locus_tag="ON345_000834"
     CDS             109..576
                     /gene="slyB"
                     /locus_tag="ON345_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR3070940.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTLFSPGQRVVLASNGSQVTVSPR"
     gene            complement(623..1057)
                     /gene="slyA"
                     /locus_tag="ON345_000835"
     CDS             complement(623..1057)
                     /gene="slyA"
                     /locus_tag="ON345_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR3070941.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            1258..1494
                     /locus_tag="ON345_000836"
     CDS             1258..1494
                     /locus_tag="ON345_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR3070942.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            1497..2354
                     /locus_tag="ON345_000837"
     CDS             1497..2354
                     /locus_tag="ON345_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR3070943.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANVNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            2354..4366
                     /locus_tag="ON345_000838"
     CDS             2354..4366
                     /locus_tag="ON345_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3070944.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVIKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(4367..4888)
                     /gene="sodC"
                     /locus_tag="ON345_000839"
     CDS             complement(4367..4888)
                     /gene="sodC"
                     /locus_tag="ON345_000839"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR3070945.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4969..5865)
                     /locus_tag="ON345_000840"
     CDS             complement(4969..5865)
                     /locus_tag="ON345_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR3070946.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5914..6153)
                     /locus_tag="ON345_000841"
     CDS             complement(5914..6153)
                     /locus_tag="ON345_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR3070947.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            6256..6855
                     /gene="nemR"
                     /locus_tag="ON345_000842"
     CDS             6256..6855
                     /gene="nemR"
                     /locus_tag="ON345_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR3070948.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKKAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6892..7989
                     /gene="nemA"
                     /locus_tag="ON345_000843"
     CDS             6892..7989
                     /gene="nemA"
                     /locus_tag="ON345_000843"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR3070949.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            8070..8477
                     /gene="gloA"
                     /locus_tag="ON345_000844"
     CDS             8070..8477
                     /gene="gloA"
                     /locus_tag="ON345_000844"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR3070950.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            8580..9227
                     /gene="rnt"
                     /locus_tag="ON345_000845"
     CDS             8580..9227
                     /gene="rnt"
                     /locus_tag="ON345_000845"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR3070951.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            9320..13936
                     /locus_tag="ON345_000846"
     CDS             9320..13936
                     /locus_tag="ON345_000846"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3070952.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWENQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVQVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(13987..14334)
                     /gene="grxD"
                     /locus_tag="ON345_000847"
     CDS             complement(13987..14334)
                     /gene="grxD"
                     /locus_tag="ON345_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR3070953.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14668..15483
                     /gene="mepH"
                     /locus_tag="ON345_000848"
     CDS             14668..15483
                     /gene="mepH"
                     /locus_tag="ON345_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR3070954.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTLSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15611..16192
                     /gene="sodB"
                     /locus_tag="ON345_000849"
     CDS             15611..16192
                     /gene="sodB"
                     /locus_tag="ON345_000849"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR3070955.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16338..17507)
                     /locus_tag="ON345_000850"
     CDS             complement(16338..17507)
                     /locus_tag="ON345_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3070956.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(17673..17762)
                     /gene="ynhF"
                     /locus_tag="ON345_000851"
     CDS             complement(17673..17762)
                     /gene="ynhF"
                     /locus_tag="ON345_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR3070957.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            18061..19086
                     /gene="purR"
                     /locus_tag="ON345_000852"
     CDS             18061..19086
                     /gene="purR"
                     /locus_tag="ON345_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR3070958.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(19083..20015)
                     /locus_tag="ON345_000853"
     CDS             complement(19083..20015)
                     /locus_tag="ON345_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3070959.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            20128..21339
                     /locus_tag="ON345_000854"
     CDS             20128..21339
                     /locus_tag="ON345_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR3070960.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            21630..22778
                     /gene="cfa"
                     /locus_tag="ON345_000855"
     CDS             21630..22778
                     /gene="cfa"
                     /locus_tag="ON345_000855"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR3070961.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(22818..23459)
                     /gene="ribE"
                     /locus_tag="ON345_000856"
     CDS             complement(22818..23459)
                     /gene="ribE"
                     /locus_tag="ON345_000856"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR3070962.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            23674..25047
                     /gene="mdtK"
                     /locus_tag="ON345_000857"
     CDS             23674..25047
                     /gene="mdtK"
                     /locus_tag="ON345_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR3070963.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALAEKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(25088..26344)
                     /locus_tag="ON345_000858"
     CDS             complement(25088..26344)
                     /locus_tag="ON345_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR3070964.1"
                     /translation="MGSDAKNLMNDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFVVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            26652..26728
                     /locus_tag="ON345_000859"
     tRNA            26652..26728
                     /locus_tag="ON345_000859"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26686..26688,aa:Val,seq:gac)
     gene            26733..26809
                     /locus_tag="ON345_000860"
     tRNA            26733..26809
                     /locus_tag="ON345_000860"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26767..26769,aa:Val,seq:gac)
     gene            26917..27222
                     /locus_tag="ON345_000861"
     CDS             26917..27222
                     /locus_tag="ON345_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR3070965.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            27348..28952
                     /locus_tag="ON345_000862"
     CDS             27348..28952
                     /locus_tag="ON345_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR3070966.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLQLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSKTFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(28964..29776)
                     /locus_tag="ON345_000863"
     CDS             complement(28964..29776)
                     /locus_tag="ON345_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070967.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(29780..30565)
                     /locus_tag="ON345_000864"
     CDS             complement(29780..30565)
                     /locus_tag="ON345_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR3070968.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(30562..31230)
                     /locus_tag="ON345_000865"
     CDS             complement(30562..31230)
                     /locus_tag="ON345_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR3070969.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(31294..31932)
                     /locus_tag="ON345_000866"
     CDS             complement(31294..31932)
                     /locus_tag="ON345_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR3070970.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(31945..34047)
                     /locus_tag="ON345_000867"
     CDS             complement(31945..34047)
                     /locus_tag="ON345_000867"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR3070971.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVLSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(34068..34694)
                     /locus_tag="ON345_000868"
     CDS             complement(34068..34694)
                     /locus_tag="ON345_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR3070972.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8393 a         9142 c         8617 g         8799 t
ORIGIN      
        1 gatttgctca tatttggtag aacatgtaac catggattca catatgccat atactttgac
       61 catgagggat gcttgcgtgg cgtttcatgg tgaacaggag atttttcaat gattaaacgc
      121 gtattggttg tttcaatggt aggtctgtct cttgtcggtt gtgttaataa cgacaccctg
      181 tcaggggatg tttataccgc ttctgaagcg aaacaagtac agaatgtcag ctatggcacc
      241 atcgttaacg tacgtccggt acagattcag ggcggtgatg attccaacgt tatcggtgca
      301 attggcggtg ctgttcttgg tggtttcctg gggaatactg ttggtggcgg aaccgggcgt
      361 tctctggcta ctgcagcagg cgctgttgca ggtggcgtag ctggtcaggg cgtacagagt
      421 gcaatgaaca aaacgcaggg tgtcgagctg gaaattcgta aagacgatgg taataccatc
      481 atggtggtac agaaacaagg caacactctt ttctctccgg gccaacgtgt cgtactggcc
      541 agcaatggca gtcaggtgac cgtttctccg cgctaaataa gtttgcgtgt ggtcaggtta
      601 ctgaccacac gcccccttca tttcaccctt tggcctgtaa ctcaatgata ttatgctcaa
      661 gttttgcgat gagcgtaatc agttgctcca gttcctctgc ggagatgcca tgtaatattt
      721 ccgcgcgggt tttgttaata acagcttcca tttcgctgat cagtggctct gccttttccg
      781 tcagtttaat acgtttagcc cgacgatcgc tggcacaagt ttgacgcgaa attaaccctt
      841 tttcttccag ttggtccaga gtacggacca gtgatggctg ctcgatgcca atcgcttttg
      901 ccagttgaat ttgcgactgg tctggaggta actgatggat attgtgtaac gtaacccaat
      961 gggtttgtgt taactccagc ggtttcaggc gatggtctat cagagcacgc catatgcgca
     1021 ccaaccgtgc cagatcagaa cctagtggcg attccaattt catctcctta taattagctt
     1081 gctaagatat tatgcggctt ttagaatagt gtgcagcaat tgtattgcta aaacaaatgt
     1141 attgctgcat ttggttaccg tcagacatat ttttcagaaa ttgcgcgtaa atttttcgca
     1201 cttaaagaat atttattaat ctaacgcaat atattcggtc gtaaaggaat ctactttgtg
     1261 aagtttatgc tcaatgcaac aggattgccc ttacaagatc tggtgttcgg tgcgtccgtc
     1321 tattttcctc cgtttttcaa agccttcgcg tttggattcg tcatctggct tgtcgtacac
     1381 cgcctgcttc gtggctggat ctacgccggt gacatctggc atcccttgtt aatggattta
     1441 tcgctgtttg cgatttgcgt ttgccttgct ctggcaatac tgattgcgtg gtaactatgt
     1501 caattaaaac aattaagtat ttctcaacaa tcattgtagc ggtagttgcg gttcttgccg
     1561 gatggtggct gtggaattat tacatgcaat ctccgtggac acgcgacggg aaaatacgcg
     1621 cagaacaggt ttctatcacc ccacaggtgt cgggacgtat tgttgagctg aatataaaag
     1681 acaaccagct ggttaacgca ggggatcttc tgctcacgat cgataaaacg ccctttcaga
     1741 tcgccgagct gaacgcacag gctcagctgg cgaaagcgca atctgacctc gcaaaagcca
     1801 ataacgaagc gaatcgtcgt cgtcatctct cacaaaattt tatttctgcc gaagaactcg
     1861 ataccgctaa cgtcaatgtt aaagcgatgc aggccagtgt taatgccgca caggccacgc
     1921 tgaaacagac acaatggcaa ctggcgcaaa cggaaattcg cgccccggtg agtggttggg
     1981 tgactaacct cactacccgc atcggcgatt acgctgacac cgggaaaccg ctgtttgccc
     2041 ttgtcgacag ccactcgttt tacgtcattg gttattttga agaaaccaag ttgcgccata
     2101 tccgcgaagg tgcaccagca caaattaccc tctatagtga taacaaaacg ttacggggtc
     2161 acgtttccag tatcggtcgg gcgatttatg atcaaagcgt tgaaagtgac tcaagcctta
     2221 ttccggatgt gaaacctaac gtcccctggg ttcgtctcgc ccaacgcgtt cccgtccgct
     2281 ttgcgctgga taaagttcca ggcgatgtca cgctggtgtc gggcaccacc tgtagcattg
     2341 ccgtaggtca ataatgaacg catcgtcatg gtccttgcgc aatttgccct ggttcaaggc
     2401 cacgctggcg caatggcgtt atgcgttacg caataccatt gccatgtgtc tggcgctgac
     2461 ggttgcctat tatttgaatc tggatgaacc ctattgggcg atgacctcgg ctgcagtggt
     2521 tagctttccc accgttggcg gtgtcatcag caaaagcctc ggacgcatcg ctggcagttt
     2581 actgggagcc attgcggcac tgcttcttgc cgggcatacg ctcaatgagc cgtggttttt
     2641 tctattgagc atgtcggcgt ggcttggctt ttgtacctgg gcctgtgcgc acttcacgaa
     2701 taacgtcgcg tatgcatttc aactggcggg ctacacggct gccatcatcg cctttccgat
     2761 ggttaatatt actgaggcca gccagctgtg ggatatcgct caggcgcgcg tttgcgaggt
     2821 gatagtcggt attttgtgcg gcggcatgat gatgatgatc ctgcctagca gttccgatgc
     2881 tacagccctt ttaaccgcat tgaaaaacat gcacgcccga ttactggaac atgccagttt
     2941 actctggcag cctgaaacaa acgatgccat tcgtgcagca catgaggggg tgattgggca
     3001 gatactgacc atgaatttgc tgcgtatcca ggctttctgg agccactatc gttttcgcca
     3061 gcaaaacgcg cgccttaatg cgctactcca ccagcaatta cgtatgacca gtgtcatctc
     3121 cagcctgcga cgtatgttgc tcaactggcc ctcaccgcca ggtgccacgc gagaaattct
     3181 cgaacaattg ctgacagcgc tcgccagttc gcaaacagat gcttacaccg tcgcacgtat
     3241 tatcgccccg ctacgcccga ccaacgtcgc cgactaccgg cacgtcgcct tctggcagcg
     3301 actacgttat ttttgccgcc tttatctgca aagtagtcag gaattacatc gtctgcaaag
     3361 cggtgtagat gatcatgcca gactcgcacg gacatccgct ctggctcgtc ataccgacaa
     3421 cgccgaagct atgtggagcg ggctgcgtac attttgtacg ttgatgatga ttggcgcatg
     3481 gagtattgca tcgcaatggg atgccggtgc caatgcatta acgctggcag caattagctg
     3541 cgtactctac tccgccgtcg cagcaccgtt taagtcgttg tcactgctga tgcgcacgct
     3601 ggtgttactt tcgctattca gctttgtgat caaatttggc ctgatggtcc agattagcga
     3661 tctgtggcaa tttttactgt ttctctttcc actgctggcg acaatgcagc ttcttaaatt
     3721 gcagatgccc aaatttgccg cactgtgggg gcaactgatt gtttttatgg gttcgtttat
     3781 cgctgtcact aatcccccgg tgtatgattt tgctgatttt cttaacgata atctggcaaa
     3841 aatcgttggc gttgcgctgg cttggctagc gttcgccatt ctgcgtccag gatcggatgc
     3901 tcgtaaaagc cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg
     3961 ccatccaaca ctgagtgaaa gcgaatttga atcgctcact tatcatcacg tcagtcagtt
     4021 gagtaacagc caggatgcgc tggctcgccg ttggttatta cgctggggtg tagtgctgct
     4081 gaactgttct catgttgtct ggcaattgcg cgactgggaa tcgcgttccg atccgttatc
     4141 gcgagtacgg gataactgta tttcactgtt gcggggagtg atgagtgagc gtggcgttca
     4201 gcaaaaatca ctggcggcca cacttgaaga attacagcgg atttgcgaca gccttgcccg
     4261 tcatcatcaa cctgccgccc gtgagctggc ggcaattgtc tggcggctgt actgctcgct
     4321 ttcgcaactt gagcaagcac caccgcaagg tacgctggcc tcgtaattac ttaattacac
     4381 cacaggcata gcgttcaccg ccaccgccca gcggtttagg ttgatcggac atattatcgc
     4441 cgccaacgtg gaccatcagc gctttgtctt tgatttcatc cagtgatttc agacgaggcg
     4501 cgatgacggc atcggtagct ttgccgtcat tattgacgac cagtgcaggc agatcgccta
     4561 aatgcccggc accttctggc ccttcatgtt taccggtatt ttgtggatca agatgcccgc
     4621 ctgcggattc cgcggcgctg gctttgccat ctttggtggc tggctggcag cttcctttgg
     4681 catgaatatg gaagccatgt tcaccggggg gtaatgcttt cagatcgggc gaaaactcca
     4741 gacctttatc ggtttcagta atggtgacgc taccaattga ctgccctacc ccttgcgacg
     4801 tgacgaggtt catctcgact ttttcactgg cagcttgtgc gccggttgca acaaccagcg
     4861 ccagaatagc cagactaaaa cgtttcatag gacctccgtt cattgtgcat tcctgaataa
     4921 gtgtaaacca gtgacacatt tttgaacggc ggctattcct aaaagtgctt acggtacgtc
     4981 gtaccccagt gccgctttac ggatacgaaa ccattgttga cgggtcattt tcagtgtttc
     5041 tgcttcgaca gctgcccgta cgcgctcaat tttacctgaa ccgataattg gcagcggctg
     5101 cgatggtaaa cgtaataccc aggcgtaaac cacctgttca atcgagcccg cgtttaactc
     5161 ctctgccacc acagccagtt catcacgcag cggctggaaa taatcatcat taaacagacg
     5221 accaccacca aggcaggacc acgccatcgg acgaacacgc agttgttgta gttggtcgag
     5281 cgtgccatcc agcagtaacg gctgatgcac cggggatatt tccacctgat tagtggcaag
     5341 ggtaaacggc agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc
     5401 aaaatgacgc actttgccgc tctgatgcag atgtttgaac gcgtccgcca cttcatcggc
     5461 atccattaac gggtctggtc ggtggattaa cagcagatcc agatgatcgg tcgcgagatt
     5521 aattagcgac tgttcggcgc tcttaatgat gtgatcgcgg tcagtgatgt aatgaccaat
     5581 gacgttttct tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt ccatccgttc
     5641 acgcaggtga ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact gatagccacc
     5701 ataaatatca gcatggtcca cggtggtcac gccgagatcc agatgctctt caataaaact
     5761 gaccagctgg cgggcggaca tattccagtc catcaatcgc cagtagccca tcacaaaacg
     5821 ggaaaactcc gggccttgcg gcgcaatagt aatacgctga accataatcg cttcctctta
     5881 tcagatatga gaggagtata cgcaagatta ggttcaaaag agtgatggtt gctccggttc
     5941 gtctgatgac gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg
     6001 cagcacctct tgtttttcgc catcgctcat tttattccag ttaaaacgct catcccgact
     6061 acgaaaacag ccgcgacaaa acccgcgttc gtcagactgg caaattcccc ggcacgggct
     6121 ctggacggga aagaactcta attgctccgc cacttcgccc tcctcagata agattattac
     6181 cattattgaa gctgttaatg tccaaagtag caactttgct tgcactagac cgactggtct
     6241 actacactcc aacgcatgaa caaacacacc gaacatgata ctcgcgaaca tctcctggcg
     6301 acgggcgagc aactttgcct gcaacgtgga ttcaccggga tggggctaag cgaattacta
     6361 aaaaccgctg aagtgccgaa agggtccttc tatcactact ttcgctctaa aaaagcgttt
     6421 ggcgttgcca tgcttgagcg tcattacgcc gcatatcacc agcgactgac tgagttgctg
     6481 caatccggcg aaggtaacta ccgcgaccgc atactggctt attaccagca aacactgaac
     6541 cagttttgcc aacatggaac catcagtggt tgcctgacag taaaactctc tgccgaagtg
     6601 tgcgatctgt cagaagatat gcgcagcgcg atggataaag gtgctcgcgg cgtgatcgcc
     6661 ctgctctctc aggcgctgga aaatggccgt gagaaccatt gtttaacctt ttgtggcgaa
     6721 ccgctacaac aggcacaagt gctttacgca ctatggctgg gtgcgaatct gcaggccaaa
     6781 atttcgcgca gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa cattattgcg
     6841 acgcctgccg tttagcaggc attttttatc accagacgac cgggagcctt tatgtcatct
     6901 gaaaaactgt attccccact gaaagtgggc gcgatcacgg cggcaaaccg tatttttatg
     6961 gcaccgctga cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc gttgatggcg
     7021 gaatactatc gccaacgtgc cagtgccggt ttgattatta gtgaagccac gcaaatttct
     7081 gcccaggcaa aaggatatgc aggtgcgcct ggcatccata gtccggagca aattgccgca
     7141 tggaaaaaaa tcaccgctgg cgttcatgct gaaaatggtc atatggccgt gcagctgtgg
     7201 cacaccggac gcatttctca cgccagcctg caacctggcg gtcaggcacc ggtagcgcct
     7261 tcagcactta gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca ggcgatccgc
     7321 gttgaaacat ccatgccgcg tgcgcttgaa ctggaagaga ttccgggtat cgtcaatgat
     7381 ttccgtcagg ccattgctaa cgcgcgtgaa gccggttttg atctggtaga gctccactct
     7441 gctcacggtt atttgctgca tcagttcctt tccccttctt caaaccatcg taccgatcag
     7501 tacggcggca gcgtggaaaa tcgcgcacgt ttggttctgg aagtggtcga tgccgggatt
     7561 gaagaatggg gtgccgatcg cattggcatt cgcgtttctc caatcggtac tttccagaac
     7621 acggataacg gcccgaatga agaagccgat gcactgtatc tgattgaaca actgggtaaa
     7681 cgcggcattg cttatctgca tatgtcagaa ccagattggg cgggaggtga accgtatact
     7741 gatgcgttcc gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca
     7801 tacacggtag aaaaagccga aacgctgatt ggcaaagggt taattgatgc ggtggcattt
     7861 ggtcgtgact ggattgcgaa cccggatctg gtcgcccgct tgcagctcaa agctgagctt
     7921 aacccacagc gtgccgaaag tttctacggt ggcggcgcgg aaggctatac cgattacccg
     7981 acgttgtaat ccaacattgc gagcggcgta aagccgccgc tatactaaaa caacattttg
     8041 aatctgttag ccattttgag gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg
     8101 gcgatttgca acgctccatc gatttttata ccaaagtgct gggcatgaaa ctgctgcgta
     8161 ccagcgaaaa cccggaatac aaatactcac tggcgtttgt tggctacggc ccggaaaccg
     8221 aagaagcggt gattgaactg acctacaact ggggcgtgga taaatacgaa ctcggcactg
     8281 cttatggtca catcgcgctt agcgtagata acgccgctga agcgtgcgaa aaaatccgtc
     8341 aaaacggggg taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact acggttatcg
     8401 cgtttgtgga agatccggac ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc
     8461 gcggtctggg caactaatct cctgccgggc gtgaactcat cgcgcccgca tctttactgc
     8521 atcgacaagt aatatttgtc ataatgcgcg ctgcaattta tccgtattaa gagaatcaga
     8581 tgtccgataa cgctcaactt accggtctgt gcgaccgttt tcgtggtttt tatcctgttg
     8641 tgatcgatgt tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt gagattgccg
     8701 ccatcaccct gaaaatggat gaacaaggct ggctgatgcc ggacaccaca ttacatttcc
     8761 acgtcgaacc atttgtcggc gcaaatttgc aaccagaagc cctcgccttc aacggcattg
     8821 acccgaacga tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac gaaattttta
     8881 aagttgtacg taaaggtatt aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca
     8941 acgccaattt tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta
     9001 acccgttcca ccctttcgcc acttttgaca ctgctgcact ggccgggctg gcactcggac
     9061 aaaccgtatt gtcaaaggct tgccagaccg ctggcatgga cttcgacagc acccaggcgc
     9121 actccgcgct gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct
     9181 ggaaacgtct gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc
     9241 tgatgacatg caatgattca ggcatctata gtgaggctat tccacgcatc aagcatgata
     9301 ttcacgggga atagcgttaa tggcagataa tccagaccct tcatcgctcc tgccggacgt
     9361 gttttcaccg gcgacccgcg actggtttct tcgcgccttt aaacagccga ccgctgtcca
     9421 gtcgcaaacc tggcatgtgg cggcgcgaag cgaacatgcg ctggtgattg caccgaccgg
     9481 ctctgggaaa acgctggcag catttctcta cgccctcgat cggctcttcc gcgaaggcgg
     9541 cgaagatacc cgcgaggcgc ataagcgtaa aacctcacgc atcctctata tttcaccgat
     9601 aaaagccctg ggaaccgacg ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga
     9661 tgaacggcgg cggcgcggcg aaacggaagt caatcttcgc gtagggatcc gtactggcga
     9721 tacgcctgca caggaacgca gcaaactcac ccgtaatccg ccggatattc tgatcaccac
     9781 acccgaatca ctctatctga tgctgacctc ccgcgcgcgc gaaacgctac gcggcgtcga
     9841 aacggtaatt attgatgaag tccacgcggt agcgggcagt aaacgtggtg cgcatctggc
     9901 gttaagtctg gagcggctcg atgcgctgct ccacacctca gcacagcgaa ttggcctttc
     9961 tgccactgtg cgctcagcca gcgatgtggc agcatttctt ggtggcgatc gcccggttac
    10021 ggtagtcaac ccgcccgcaa tgcgccatcc gcagatacga attgtcgtac cggtcgccaa
    10081 tatggatgat gtctcatcgg tcgccagcgg caccggcgaa gacagccatg ccggccggga
    10141 aggctccatc tggccatata ttgaaacggg tatccttgat gaagtgttgc gccatcgctc
    10201 gaccattgtc tttactaatt cacgcgggct ggcggaaaaa ctgacggcac gattaaatga
    10261 gctttacgcc gcacgcttac agcgttcccc gtctatcgcc gttgatgcgg cccatttcga
    10321 gtcgacctcc ggcgcaacct ctaaccgtgt acaaagtagc gacgttttta ttgcccgctc
    10381 acaccacggc tccgtctcta aagaacaacg agcaatcacc gaacaggcgc tgaaatcggg
    10441 tgaattacgc tgcgtggtcg caacctccag tcttgaactg gggattgata tgggcgcggt
    10501 ggatctagtg attcaggtgg caacgccgct ttctgttgcc agtgggttac aacgcattgg
    10561 tcgcgccgga catcaggttg gcggtgtatc taaagggctg tttttccccc gtacccggcg
    10621 tgatttagtc gattccgcag tcattgtaga gtgtatgttc gcaggcaggc tggaaaacct
    10681 gacaccaccg cataatcctc tcgacgtcct tgcgcagcaa accgttgccg ccgcggcgat
    10741 ggatgcatta caggtagacg aatggtactc ccgcgtacgc cgtgccgcac cgtggaaaga
    10801 tctgccaaga cgtgtttttg acgccacgct ggatatgctt tccgggcgct atccctctgg
    10861 cgatttttct gcttttcgcc ccaaactggt ctggaaccgg gagaccggga tattgaccgc
    10921 ccgacctggc gctcaattgt tggcggttac cagcggtggc accattccgg atcgtggcat
    10981 gtatagcgtg ttattacccg aaggtgaaga acaggccggt tcgcggcggg tgggtgaact
    11041 ggatgaggag atggtatatg agtcgcgggt gaacgacatt atcactctcg gcgctacctc
    11101 atggcggatc cagcaaatca cccgcgatca ggtgattgtg actcctgctc cgggtcgttc
    11161 tgcccggctc cccttctggc gtggtgaagg taacggacgt ccggctgaat taggcgagat
    11221 gatcggagat tttcttcatt tgctggcgga tggcgcgttc ttttccggga ctattccccc
    11281 gtggctggca gaagaaaata cgatcgccaa tattcagggg ttgattgacg agcaacgcaa
    11341 cgcgacgggc atcgttccgg ggagtcgcca tctggtcctc gaacggtgcc gtgatgaaat
    11401 tggcgactgg cgtattattt tgcactctcc ctatggaaga cgggtgcatg aaccctgggc
    11461 gctggcgatt gccgggcgaa tacatgccct atggggcgct gacgcgtctg tggtcgccag
    11521 tgatgacggc attgttgcgc gcattcctga cactgatggt aaattgcccg acgccgcgat
    11581 ttttttgttt gaaccagaaa agttgctgca aattgtccgc gaggcggtag gcagctcggc
    11641 acttttcgcc gcccgttttc gcgaatgcgc cgcgcgggca ttattaatgc cggggcgcac
    11701 tccgggccat cgcaccccgc tttggcaaca acggctgcgc gccagtcagt tgctggaaat
    11761 cgctcaggga tatccggatt ttccggtcat tctcgaaacc ctacgcgaat gtctgcaaga
    11821 tgtttatgat cttcccgcac tggaacgttt gatgcgtcgc ctgaacggtg gcgaaattca
    11881 aatatccgat gtaacgacca ctacgccctc gcctttcgcc acaagtttat tgttcggcta
    11941 tgtcgcggaa tttatgtacc agagcgacgc cccgctggca gagcgccggg catccgtact
    12001 gtcgctggac agcgagttac tgcgcaatct actcggacag gtcgatccgg gggaattact
    12061 cgacccgcag gtcattcgcc aggtggaaga agagttgcaa cgactggctc ctggcagaag
    12121 agcgaaaggt gaagaaggat tgttcgacct gctgcgcgaa ctggggccaa tgaccgttga
    12181 agacctggcg caacggcata caggcagcag tgaagaggtt gcgtcgtatc tggaaaatct
    12241 tcttgcagta aaacgaatct tcccagcgat gattagcgga caggagcgtc ttgcctgtat
    12301 ggatgatgcc gccaggctgc gtgatgccct cggcgtacga ctaccagagt cattgccaga
    12361 gatttattta catagagtca gttacccgct tcgcgacctc tttctgcgct atctccgggc
    12421 tcatgctctg gtcacggctg aacaactggc tcatgagttt agtctcggta ttgccattgt
    12481 cgaagagcag cttcagcaac tgcgtgaaca gggtctggtg atgaatctgc aacaagacat
    12541 ctgggtgagc gatgaagtat ttcgtcgtct gcgtttgcgc tcgctgcaag ccgccagaga
    12601 agcgacgcgt cccgttgcag ccacgaccta tgcgcgattg ctgctggaac gtcagggcgt
    12661 attacccgcc accgatggta gcccggcgct ctttgcctca acatcgccag gcgtttatga
    12721 gggcgtagat ggcgtgatgc gggtgatcga acagcttgcc ggagtcggtt tacccgcctc
    12781 actctgggaa aaccagatcc tgcctgcccg cgtacgcgac tattcgccag aaatgctcga
    12841 tgaattactg gcaaccggag cggttatctg gtcgggacaa aaaaagctgg gtgaagatga
    12901 cggcctggtg gcactgcatc tacaggaata tgccgcagaa tcgttcactc ccgccgaagc
    12961 ggatcaggcg aatcgttcgg cgctgcagca agcgatagtc caggttctgg ctgacggagg
    13021 agcctggttt gcacaacaaa tcagccaacg gatacgcgat aaaatcggcg aatcggttga
    13081 tccctcggca ctgcaagagg cgttatgggc gctggtctgg caaggcgtca tcaccagcga
    13141 tatttgggca ccgttacgcg ccctcacccg cagcagttcc aacgcacgca cctcaactcg
    13201 ccgcagtcac cgggctcgtc gtgggcgtcc tgtctatgca caacccgtct cgccgcgggt
    13261 gtcttacaac acaccaaatc tggctggacg ctggtcgtta ttgcaggtgg agccactaaa
    13321 cgataccgaa aggatgctgg cgctggcgga aaatatgctc gaccgctacg gcatcatcag
    13381 tcgtcaggcg gtgatagccg aaaatatccc tggcgggttt ccatcgatgc aaacgctttg
    13441 tcgaagtatg gaagactccg ggcgaattat gcgaggtcgt tttgtagaag gtctgggtgg
    13501 cgcgcaattc gctgaacgtc tgactattga ccgattgcgc gatctggcga cacaagccac
    13561 gcaaacgcgc cactatacac cagtggcgct ctctgccaac gatccggcta atgtgtgggg
    13621 aaatcttctg ccctggcctg cacatccggc aacgctggtt ccaacgcgtc gggcgggtgc
    13681 gctggtggtc gtttctggcg gcaaattgtt actctatctg gcgcaaggtg gcaaaaaaat
    13741 gctggtctgg caggaaaaag aggaattact cgccccagag gttttccacg cgctgactac
    13801 cgcactgcgt cgcgaaccac ggctgcgctt tacgctaaca gaagtgaatg atctaccggt
    13861 ccggcaaacg ccgatgttta cgctgctgcg tgaggcggga ttttcaagtt cgccacaagg
    13921 gctggattgg ggatagagaa aggactgacg gatgcctgtt cgcatccgtc agtattgcag
    13981 gacggattat tccgcgtccg gctcttcaga cttgtattta gcggcagttt ctttgatcag
    14041 ctgctgcagt tcgccacgct gatacatttc gatcacgata tcacaaccgc ctaccagctc
    14101 gccgtcaacc cacagttgcg ggaaggtcgg ccagttagca tatttaggca gttccgcacg
    14161 aatgtccggg ttctgcagaa tatcaacata ggcaaaacgt tcgccacatg cggcaagcgc
    14221 ctggactgcc tgggcagaga aaccgcagct cggcagtttc ggtgaacctt tcatgtacag
    14281 caggatcggg ttttcagcaa tctggcgttg gattttttcg atagtggtgc tcattgtctt
    14341 gcttcctcaa acttctttac ggcagtaatc tgacattgta gcgggtcagt gcggcatcgg
    14401 aaaataacat tttcatcacg cttttgctat tttatccctt tgctctattt gttgcatttc
    14461 aaatattcgt ttcttctctg atgccggcaa actggcgtat ttataaccat ttttagcttt
    14521 cactgctgca tttttttgcg ctcgccaacg aaacgtattt tttaacaata aaagctatta
    14581 actttctctt cttctatgca ttagaatcat caagttttgt aaatcagacg caggcatgat
    14641 agacctgcct taacagaggg acgctcagtg gcgcggataa accgtatttc gatcacgctc
    14701 tgtgctttac tttttaccac cctgccttta acgcctatgg cccatgcttc aaagcaagcc
    14761 agggagagtt ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa aagcacggca
    14821 accaccaaaa agacccagaa aacagcgaaa aaagccgcca gtaaaagtac gaccaaaagc
    14881 aaaaccgctt cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc caaaactctg
    14941 agcaaacacg ccgtcaataa aacggcctca gccagcttta ccgaaaagtg taccaaacgt
    15001 aagggctata aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac tcttgccgac
    15061 gcgcacaaag cgaaggtgca aaaagctacg aaagtggcaa tgaataaact gatgcagcaa
    15121 attggtaagc catatcgttg gggtggcagc tcaccgcgta ccggttttga ttgcagcggc
    15181 ctggtttatt acgcttataa agatttggtg aaaattcgta ttccgcgtac ggcgaatgaa
    15241 atgtatcacc tgcgtgatgc agcgccaatc gaacgtagtg aactgaaaaa cggcgacctg
    15301 gtctttttcc gtactcaggg acgcggcaca gccgatcatg tcggcgtgta tgtcggcaac
    15361 ggcaaattta ttcagtcacc gcgcacaggt caggaaattc aaatcacttc tctcagtgaa
    15421 gactactggc agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa aacacttcgc
    15481 taaaacttta ccctgttgtt acggcaacag ggtaagttca tcttttgtct caccttttaa
    15541 tttgctaccc tatccatacg cacaataagg ctattgtacg tatgcaaatt aataataaag
    15601 gagagtagca atgtcattcg aattacctgc attaccatat gctaaagatg ctctggcacc
    15661 gcacatttct gcggaaacta tcgagtatca ctacggcaag catcatcaga cttatgtcac
    15721 taacctgaac aacctgatta aaggtaccgc gtttgaaggt aaatcactgg aagagattat
    15781 tcgcagctct gaaggtggcg tattcaacaa cgcagctcag gtctggaacc atactttcta
    15841 ctggaactgc ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg ctgaagctat
    15901 cgccgcatct tttggcagct ttgccgattt caaagcgcag tttactgatg cagcgatcaa
    15961 aaactttggt tctggctgga cctggctggt gaaaaacagc gatggcaaac tggctatcgt
    16021 ttcaacctct aacgcgggta ctccgctgac caccgatgcg actccgctgc tgaccgttga
    16081 tgtctgggaa cacgcttatt acatcgacta tcgcaatgca cgtcctggct atctggagca
    16141 cttctgggcg ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat aataactgat
    16201 ggcaaatgca gcattgcctg atgcgcttcg cttaccatgc caacgcggat gaggcgttaa
    16261 cagcgcattc ggcaagataa atcgcacgtt gtcagcaact gtaacgcaga aggttatcct
    16321 tctgcgtttt tgtttaatta gctgttagca acgcaaactg tttcaggttg ttttctggct
    16381 gacataaaca ccagcaataa tgccagtccc gcgacaatcg ctcccatcac cggcacaaag
    16441 ctgtatccca gcccagcgga aattaccgca ccaccagcag ctgctcccag cgcatttcca
    16501 agattaaagg caccaatatt gactgatgaa gacagacctg gcgcttcact ggcgacacgc
    16561 atcacgcgca tctgtaacgg cggtacgacc gcaaaggttg ctgcgcccca caccaccatg
    16621 ctaatagctg cgccgaactc attgcgggcc aggaacggga ttgccagcat aatcaccatc
    16681 agcaacaaca aaaagccttt caacgtgccg ttaactgaac gatctgccag tttgccgccg
    16741 agatagttac cgatagagaa tccgacacca atcagcacca gcattgccgt gacgaacacc
    16801 ggtgttgcgt gggtaatact ttgcagtacc ggagagatat aggtgtagag agtaaacatt
    16861 gcaccagctc ccagtaccgt cgtcagcaat gcagacagca cctgcggacg cattaatacc
    16921 gccagctctt ttttcacttc aggtcgtgcc cctgcaccac ctttaggtaa tgagaagaac
    16981 agacttacca ttgaaatcac tcccagcccc gccgttgcca gaaatgacat ccgccagccg
    17041 atggtttcac ccaaccaggt cgccgccggc acgccaccga tatttgccag ggttaacccc
    17101 ataaacatag tggcaactgc gctggcctgt ttatgttttg gcaccacgct tgcggccacg
    17161 actgaaccca aaccaaaaaa tgctccgtga ttcaggctgg tcaaaatgcg tgaaagcatc
    17221 agggtcatat aatccggcgc gatggcggaa agtacgttgc cgagcgtgaa aattgccatc
    17281 aggaaaatca acgcactgcg gcgggcacga tgagaaagta gaagcgtcat cagcggcgcg
    17341 cccaccatta cgccaactgc ataggcactg attaacattc cggcagcggg aatcgagaca
    17401 tccacaccgc gcgcaatgac gggcaacaag cccattggcg agaactccgt tgtcccgata
    17461 ccaaacgcgc caatcgccag cgccagcaac ggatagttaa ttttcatgcc ttatctccac
    17521 ctcttcgcgt cattacgcga tattcattaa agtggcgaaa gcatgacagc aatcacaaaa
    17581 aaatgaaaat aacaaaaaga gaaaacactt ttgccatttt gctaacaaac aggaaggaga
    17641 tgcgagggag aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt caaacccacg
    17701 gctacgatca aaccgaggac gataatcgtt gttaccagtg aaaatttaag gtcggtgctc
    17761 atcaagtttt ctcctttttt attaccacac aaaaagtgat attacgcatt tttacacact
    17821 gtgatgaaaa aatctcccgt catttataat gataagtgtt tttaccactt ccccttttcg
    17881 tcaagatcgg ccaaaattcc acgcttacac tatttgcgta ctggccattg accccttcct
    17941 gacgctccgt gtcgtttttc cggcgtaccg caacactttt gttgtgcgta aggtgtgtaa
    18001 aggcaaacgt ttaccttgcg attttgcagg agctgaagtt agggtctgga gtgaaatgga
    18061 atggcaacaa taaaagatgt agcgaaacga gcaaacgttt ccactacaac tgtgtcacac
    18121 gtaatcaaca aaacacgttt cgtcgctgaa gaaacacgca acgccgtgtg ggcagcgatt
    18181 aaagaattac actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa ccacaccaag
    18241 tctatcggtt tgctggcgac cagcagcgaa gcggcctatt ttgccgagat cattgaagcg
    18301 gttgaaaaaa attgcttcca gaaaggttac accctgattc tgggtaatgc gtggaacaat
    18361 cttgagaaac agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga tggtctgctg
    18421 gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc tggaagagta tcgccatatc
    18481 ccaatggtgg tgatggactg gggtgaagca aaagctgact tcaccgatgc ggtcattgat
    18541 aacgcgttcg aaggcggcta catggccggg cgttatctga ttgaacgcgg tcaccgcgaa
    18601 atcggcgtca tccccggccc gctggaacgt aacaccggcg caggccgcct tgccggtttt
    18661 atgaaggcga tggaagaagc gatgatcaag gtgccggaaa gctggattgt gcagggtgac
    18721 tttgaacccg aatctggtta tcgcgccatg cagcaaatac tgtcgcagcc gcatcgccct
    18781 actgccgtct tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc tgctgatgaa
    18841 atgggtctgc gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt gcgcaacgcc
    18901 cgctatttta cgccggcgct gaccacgatc caccagccaa aagattcgct gggtgaaaca
    18961 gcgttcaaca tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca gtccattgaa
    19021 gtgcatccgc gtttgattga acgccgctcc gtggctgacg gcccgttccg cgactatcgt
    19081 cgttaatcac ccgttgcggg agtctcttcc ggctcccgca gccactcctt attcagcgtc
    19141 tcactatcgc cgagatactc aagcagccag gttaacgcag gcgacatatc attttgctgc
    19201 catgtcagac aacatgccga atccgggaag gggttttcca gttcaagtgc tacccacttc
    19261 ccctcattaa gccacggttt ggcgaaatgt gttggcacca tccctatgca taatcctgcc
    19321 gagatacagg ttgccgatga ttcccagtca ggcacgacaa ctcttttttg gttatccagc
    19381 aaccaggtaa tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa cgacggccag
    19441 ttgcgcaacg tatcatcgct gaatgggcca tccatcaacg ccagcgggtg gtggctggca
    19501 acaacgcaac tccagcttag catccccata tcccggaagg cataacgacc gcctaccgga
    19561 atcgcgcgtg ttgcgccaat cgccagttcc acgcgcccgt cggaaagcgc atcccagaca
    19621 ccgttgaaca cttcctgaaa gacaagaagt tcgacatcat caaaatggcg ataaaaatca
    19681 acgatcatct gccgtgtacg ttctggcctg acaatattat ccactgcgat agctaactga
    19741 ccgcgccagc cgttcgctat ctgctgacat tgctggcggg tgatctgcat ttttttgaca
    19801 acagagcgcc cttctttgag aaaccacgct ccagcagcgg tcagctccac atcacggtgc
    19861 cgtcgttcaa agagcggcac cgccagccac tcttccagct gacgcacggt atagctgacc
    19921 gcagaaggaa cgcgatgcag ctcctgtgcc gcagcgctaa aactaccatt acgcgctacc
    19981 gcatcaacaa cttcgagtga atattctgac cacatagtct gcctgcaaaa tttttgaaat
    20041 cagtcatcaa atattaccgt ttcacaacac taatttcact ccctacactt tgcggcggtg
    20101 tttaattgag agatttagag aatatacatg caacctggga aaagattttt agtctggctg
    20161 gcgggtttga gcgtactcgg ttttctggca accgatatgt atctgcctgc tttcgccgcc
    20221 atacaggccg acctgcaaac gcctgcgtct gctgtcagtg ccagccttag tctgttcctt
    20281 gccggttttg ccgcagccca gcttctgtgg gggccactct ccgaccgtta tggtcgtaaa
    20341 ccggtgttat taatcggcct gacaattttt gcgttaggta gtctggggat gctgtgggta
    20401 gaaaacgccg ctacgctgct ggtattgcgt tttgtacagg ctgtgggtgt ctgcgccgcg
    20461 gcggttatct ggcaagcgtt agtgacggat tattatcctt cacagaaagt taaccgaatt
    20521 tttgcgacca tcatgccgct ggtgggtcta tctccggccc tggctcctct gttaggaagc
    20581 tggctgctgg tccatttttc ctggcaggcg attttcgcca ccctgtttgc cattaccgtg
    20641 gtgctgattc tgcctatttt ctggctcaaa cccacgacga aggcccgtaa caatagtcag
    20701 gatggtctga cctttaccga cctgctacgt tctaaaacct atcgcggcaa cgtgctgata
    20761 tacgcggcct gttcagccag tttttttgca tggctgaccg gttcaccgtt catccttagt
    20821 gaaatgggct acagcccggc agttattggt ttaagttatg tcccgcaaac tatcgcgttt
    20881 ctgattggtg gttatggctg tcgcgccgcg ctgcagaaat ggcaaggcaa gcagttatta
    20941 ccgtggttgc tggtgctgtt tgcagtcagc gtcattgcga cctgggctgc aggcttcatt
    21001 agccatgtgt cgctggtcga aatcctgatc ccattctgtg tgatggcgat tgccaatggc
    21061 gcgatctacc cgattgttgt cgcccaggcg ctgcgtccct tcccacacgc aactggtcgc
    21121 gccgcagcgt tgcagaacac tctacaactg ggtctgtgct tcctcgcaag tctggtagtt
    21181 tcctggctga tcagtatcag cacgccattg ctcaccacca ccagcgtgat gttatcaaca
    21241 gtagtgctgg tcgctctggg ttacatgatg caacgttgtg aagaagttgg ctgccagaat
    21301 catggcaatg ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat atacttatac
    21361 ttaggctgct aacaaaattt tgttgtatga ttgaaattag cggcctatac taatttcgag
    21421 ttgttaaagc tacgataaat attatgtttt tacggggaca ggatcgttcc cgactcacta
    21481 tggatggtca tttcggcaag ggttcctcct ttccctctgt tctacgtcgg attatagact
    21541 cgcggttttt tctgcgggat ttctcacaaa gcccaaaaag cgtctacgct gttttaaggt
    21601 tctgatcacc gaccagtgat ggagaaacta tgagttcatc gtgtatagaa gaagtcagtg
    21661 taccggatga caactggtac cgtatcgcca acgaattact tagccgtgcc ggtatagcca
    21721 ttaacggttc tgccccggct gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc
    21781 tgcaagaagg ctctttgggg ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc
    21841 gactggatat gttttttagc aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc
    21901 atttcaaaga cacgctacgt attgccggcg ctcgtctctt caatctgcag agtaaaaaac
    21961 gtgcctggat agtcggcaaa gagcattacg atttgggtaa tgacttgttc agccgcatgc
    22021 ttgatccctt catgcaatat tcctgcgctt actggaaaga tgccgataat ctggaatctg
    22081 cccagcaggc gaagctcaaa atgatttgtg aaaaattgca gttaaaacca gggatgcgcg
    22141 tactggatat tggctgcggc tggggcggac tggcacacta tatggcatct aactatgacg
    22201 taagcgtggt gggcgtcacc atttctgccg aacagcaaaa aatggctcag gaacgctgtg
    22261 aaggcctgga tgtcaccatt ttgctgcaag attatcgtga cctgaacgac cagtttgatc
    22321 gtattgtttc tgtggggatg ttcgagcacg tcggaccgaa aaattacgat acctattttg
    22381 cggtggtgga tcgtaatctg aaaccggaag gcatattcct gctccatact atcggttcga
    22441 aaaaaaccga tctgaatgtt gatccctgga ttaataaata tatttttccg aacggttgcc
    22501 tgccctctgt acgccagatt gctcaatcca gcgaacccca ctttgtgatg gaagactggc
    22561 ataacttcgg tgctgattac gatactacgt tgatggcgtg gtatgaacga ttcctcgccg
    22621 catggccaga aattgcggat aactacagtg aacgctttaa acgaatgttt acctattatc
    22681 tgaatgcctg tgcaggtgct ttccgcgccc gtgatattca gctctggcag gtcgtgttct
    22741 cacgcggtgt tgaaaacggc cttcgagtcg ctcgctaaag gctattctat cgccccctct
    22801 ccgggggcga tttcagatca ggcttctgta cctggttgat tcatggcatt ttctcgtgcc
    22861 gccagcacac gttctaccgt atctaccact gcctgagttt gtggatcaat ttcaatgttg
    22921 acgcgtgcgc cgagtttttt cttcccaaga gtcgtgcgtt ccagagtttc cggaattaaa
    22981 tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca
    23041 ataaatcctt tgtacagaat atatttcatc aactgactat cctggacttt aaaccagatc
    23101 tggcgattat tttctgaggt taatattttc gccacttcag cagtggtcat aatatgacct
    23161 gacattaagt gtccgccaat ttcatcactg aatttcgccg cacgctcaac gtttacccaa
    23221 tcccccactt ttaaatcgcc aagattggta atgcgtaacg tttctttcat caggtcaaaa
    23281 ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa
    23341 gcaccggttt ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa
    23401 tttggtttct cgtcaatcga caccagtttt gcggtgccct gtacaatccc cgtaaacata
    23461 cttacaactc ctgaaatcag ttaagacatt ctgttcagca caatagcagg tggaaaacgc
    23521 ccttaccagt gaaggggtaa gaatggctat tttttcactg gagaattaat aaatcctcgc
    23581 tacaatagac tgaatttccc ctgcttcttc tttttgctgc ccattcaggc ggctttttag
    23641 tctctcatat aactacaaat aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt
    23701 ctgttattag cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt
    23761 tttgtcgata ccgtgatggc gggcggctat agtgccaccg acatggcggc ggtcgctatc
    23821 ggtacttcta tctggcttcc ggcgatcctc tttggtcacg gactgctgct ggcattaacg
    23881 ccggttatcg cgcaattaaa tggttccggt cgacgtgagc gcattgcgca tcaggtgcga
    23941 caaggtttct ggctggcagg ttttgtttcc gttctcatta tgctggtgct gtggaatgca
    24001 ggttacatta tccgctccat ggaaaacatc gatccggctc tggcggagaa agccgtgggt
    24061 tatctgcgtg cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt tgcccgtaac
    24121 cagtgtgaag gtctggcaaa aaccaagccg ggtatggtaa tgggctttat cggcctgctg
    24181 gtgaacatcc cggtgaacta tatctttatt tatggtcatt tcggtatgcc tgagctcggt
    24241 ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg ttatgttcct tgccatggtt
    24301 tcttacatta aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg caccgcgaaa
    24361 cccgatcctg cggttatgaa acgactgatt caactcggtt tgccgattgc gctggcacta
    24421 ttctttgaag tgacactgtt tgccgtcgtg gctctgttag tgtctccgct cggtattgtt
    24481 gatgtcgcag gacaccagat tgccctgaac tttagttcac taatgttcgt gcttccaatg
    24541 tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg
    24601 gatgcgcaaa ccgctgctcg gaccgggctt atggtgggtg tctgtatggc aaccctgacg
    24661 gccattttca cggtttcact gcgggagcaa atcgccctgt tgtacaacga caatcccgag
    24721 gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg tatatcagat ttctgactca
    24781 atccaggtga ttggcagtgg gattttgcgt ggttataaag atacgcgttc cattttctat
    24841 atcaccttta cagcttactg ggtgctgggc ttgccaagcg gctatattct ggcactgacc
    24901 gatctggtcg ttgaacctat ggggccagca ggcttctgga taggctttat tatcggcctg
    24961 acatcggcag ccattatgat gatgctgcgt atgcggttcc tgcaacgtat gccgtcagcc
    25021 atcattctgc aacgagcgtc ccgctaataa agacaaggcg caaccttcac gggttgcgcc
    25081 tgtgttttta cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca
    25141 gagcgttaca cagagtttgc cgactggcat ctgttatctc aacgtgtata tccccggtaa
    25201 cttccccttt cacattgatg aaatcaggtg aaatttcttt cgcactggtt tgcaggcaaa
    25261 tcgctccctc tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc gcaccagtga
    25321 tcagccacag ttttccgcca ttcagattga tgttaccaac aacaaaagtg acaccgtcac
    25381 cttttaccgt actgccgtcg agaatcaaca caccgccctg ctgtacagta atcgttgatt
    25441 gttgcagatc tgcgccatag ccaataatca ttgaagcgcc gtccagtaat gcaacactcc
    25501 cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg
    25561 gtgacaacag gatcactgtt ccaccttgta ttaccgcatt ttccagcgta ccggcatgca
    25621 cttcagcccg accacccaca ttcacggaca ggttttgcgt cttaccggaa ctgtagagct
    25681 gaaggccatc cgtccccaga cgataaatgg atccattctc aacaatagtg tcagagtctt
    25741 caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct ttcccggcca
    25801 gcataaaaac gccgccctga ttaatctcac aattgctggc ttccccacct ggatacacca
    25861 acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc aacaggccgc
    25921 cctcttgatc gagaatgatt ttttccgcgc gatgtccttc cagtacacgc aggttaccgc
    25981 cattttccag caacaaaccg caggcataac cctgatcaac gctgaattca ccttcgggat
    26041 ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg gactgaacaa
    26101 tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc
    26161 atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc acggtatttt
    26221 cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc gcgccaatga
    26281 cctcgccggt cttaacaatt tgcacattcc cgtcgttcat caagtttttc gcatcagatc
    26341 ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt gtaaaggcta
    26401 cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt aaactctgat
    26461 acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat cgcccttgca
    26521 aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa
    26581 aattacgttt tgcctcttgc caccttccca ctctgccgct aatattcgtc cccgttgtca
    26641 cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat ggtgggggtc
    26701 gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg gttagagcac
    26761 caccttgaca tggtgggggt cggtggttcg agtccactca gacgcaccag attttcttaa
    26821 tctggtattc tcctttttcc ctctgtttct tctctgtatc caatacgtta aaagatttac
    26881 actgtcttcg tatgcgttat cagaaggaga atcgctatgg caactttgtt acaacttcat
    26941 tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc gcttgctgag
    27001 tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga aaagaaccac
    27061 gaagccggtg ggatctatct ttttactgat gaaaaaagcg cccttgccta tctggaaaaa
    27121 catactgccc gacttaaaaa tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc
    27181 aatgagccac tgagtcaaat caatcaggca aaactcgcct gacagaattt aatcaagggc
    27241 ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac
    27301 ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg
    27361 ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta cagcaacaaa
    27421 ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg atgccataca
    27481 gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa ataccgccga
    27541 ttaattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc cagcgttatg
    27601 gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg ctgggcgaat
    27661 atttccgcga tcaattttta caattagtag accaggcacg aaagcaaaaa tttgcagtgg
    27721 ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc gtcatgctcg
    27781 ctacaaatca ggatttaccc agcaagacgt ttgatttagc ggtgattgcc acgggtcacg
    27841 tctggcctga tgaagaagaa gcaacccgaa cgtattttcc cagcccgtgg tcaggcctga
    27901 tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg agcggactgg
    27961 atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat aaacaacacg
    28021 tcgtttttca ccgcgataac gcaagtgaaa agctaaatat cacgttgatg tcgcgcacgg
    28081 gtattttacc cgaagccgat ttctattgcc ctattcccta cgagccctta cacatcgtca
    28141 ccgatcaggc attaaatgct gagattcaaa aaggcgaata tggccttttg gatcgggtat
    28201 ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa cgcatagcct
    28261 tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg
    28321 acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac
    28381 atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag gaaattgttc
    28441 cacatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgcccgg gttttcatcg
    28501 ataattatgc ggcaatccct tcagagtcta ttcgtcggct gctggcctta cgtgaagcgg
    28561 gaatcattca tattctcgcc ctcggtgaag actacaaaat ggaaattaat gagtcgcgca
    28621 ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttattgat gcccgcggac
    28681 agcgtccgct taaagtgaaa gatattcctt tccctgggct acgcgagcaa ttacagaaaa
    28741 caggggatga aatccctgat gttggcgaag attatacgtt acagcaaccc gaagatattc
    28801 gtggacgcgt agcgttcggc gcgttgccct ggttgatgca cgaccagcct ttcgttcagg
    28861 gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta aagcctgcat
    28921 cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact
    28981 gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca ccttcaatgg
    29041 cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt ccccctggcc
    29101 cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca ccgctggcaa
    29161 tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt
    29221 gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa acgcgtactc
    29281 gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc gaagaattac
    29341 cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta aagccgacac
    29401 ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca gcggttgcga
    29461 ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac
    29521 tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa acttcctgat
    29581 gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga ctctccgccc
    29641 agtccgtata tccttcctca atcagtgcat gatgaatatc ggcataacta ccgccgcgtg
    29701 ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct cgcaggtctg
    29761 ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt ttaaaggttt
    29821 cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa aagaggctta
    29881 taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc
    29941 ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc agcaatccat
    30001 acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt gttgttgccg
    30061 ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg
    30121 ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc ccaacggcat
    30181 tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tccgcaaact tcatgcaccg
    30241 caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc agcccacttg
    30301 ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgctgt
    30361 aaagataaac cttctcacca tgatcggtgg ctgatttctt cacgccacga gcacgaagca
    30421 aggcgtgcag ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc
    30481 acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg ctctgaaact
    30541 gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg
    30601 gcgatacaga tgcggtttcc cggcaccagg tagctgatat tgatagtatt tattatcctg
    30661 caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccggaca
    30721 ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca
    30781 tttatctgcc actttagtga cagggttaag gtagcgaacc tggtacggac aagcgccaat
    30841 gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg
    30901 ccacgatgcc cccgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga
    30961 ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat
    31021 cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc gggcgcaaat
    31081 attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg
    31141 cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc
    31201 cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag tggctgcaac
    31261 tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc
    31321 gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta
    31381 caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat
    31441 cgcggcgatc gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat
    31501 ggcgactttg taaggacgag gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc
    31561 gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc
    31621 ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat ccgcgtagtc
    31681 tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg
    31741 tggcgagaga aacacggtgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa
    31801 ctcagccagg atttcagcag gttctggttc ggttaccgta tcaacggggt gacgcatccc
    31861 ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc
    31921 ctgatggttc atcttaccca ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc
    31981 ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gttggacaac caagctgtgg
    32041 gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat
    32101 tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaata cccaggaaca
    32161 gataagatcg tgttcattac gcatatcttt ggtttgcatc agttttaccg tgtaggcacg
    32221 atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc
    32281 ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt
    32341 cagcggagaa acggtcattg gccagaccca gttacatagg gtgacggcgt tatgcaaaca
    32401 gacccgcaac agcgaccact tagcatattt aatttttgcg tcattgattg gcgtgtagtt
    32461 tttggtttca tcgtaagcat cttcagaacc aaaaagttct ttcgccactt cacgttgcag
    32521 tttcaatggc aagccggaac caataaagtt gatatgggta tgcgtcatgc agtcacggtt
    32581 gaacatacag ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg
    32641 atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt cacccaaatt
    32701 ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac ccacacgatg
    32761 cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca gttgatccca
    32821 gcgaatttcc gcatactctt cagcaggcag aacacgcttg aacacacctt tgctgtaaca
    32881 ataggtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt catcgaacag
    32941 attcagaccc accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg
    33001 accgttcggg aagatggtgg tatggacaaa gttggctaca caagtgttac cacctgtgct
    33061 gagcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca
    33121 agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta agtcaaaaac
    33181 ggatttatag gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt cacccgtttc
    33241 aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc agcgtgactt
    33301 gggatctgaa tactctgccc acgattgtgg cgtacttggc acgacatggt tgttattcgc
    33361 accgataagt tcagtcatca tgtaatcgtt gagacgcttc atctcctgac gatcggcaat
    33421 gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccattattgc
    33481 gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaacaag
    33541 gttttcccca gcctgaccaa tagccgccac acaggtttcc ggactggtca agcgacaaat
    33601 ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tctgcttttt ccaggctaac
    33661 tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac
    33721 gtcgtatcca gcgaatttca tttgcgccgc aaaaaagccc cccatatggg catcgacgac
    33781 taaatttcct ttggtaaaag tagaaagtga ggtgatattt acgcgagaac tacagggggc
    33841 accagatcca gttaatgggc cggtagcaaa aactaattta ttcgcttcat cgaaaggttt
    33901 cgtgcctggc ggtacttcgt catacataat tttgtagccg aagcccatgc caccgacaaa
    33961 acttttaaac ttactggaat cttcgagggt aatatttcct gtcgtgagat tgactcttaa
    34021 tatattacct gtccaaccgt tagccatgat tttttccttt gcaagattca cacagtaata
    34081 tctttccact cgataatttt taacgcccct gttgggcagg cgtttgcgca ttccccgcat
    34141 aacacacatt tcgaggattt tttactttcg gtatttacgg tggccatcat ccacgggcat
    34201 gccgtagtac aggcgctaca gccaatacaa cgtttatgat cgacggtaat acagccttct
    34261 ttctgctgcc aggtaatcgc accaatcggg cagacgttca tgcattgcgg ctctttgcat
    34321 tgacggcagg tgtccgcggt atagttgaga tcgccataca ggccgccgcc agagccaacc
    34381 ccgttgtcgc caaagaaata attgcggtgg attttgatac gggagaagaa tgtccctact
    34441 gagccatcgt tgaagttggt acaagagatt tcacagcggt ggcaacccgt gcatcgcgct
    34501 cgctgggtca ccagcacccc tttgggtgta tttatcaacc ctactgtgcc gctatcaatg
    34561 tcttcctgtt tgcaacccaa aagtgacagt aacgcagggg caattgttag accagcaagg
    34621 ccttttcctg atatacgcaa gaattctaat cgcgttaagc caatatctaa tagtggacga
    34681 tcaaccgggt tcatttattt cgcatcctct tcgcaaacac agtgctgaca attcagcaga
    34741 ctgatattaa gcattcaact aatacgctgc tgactctgtc ctcaacacca gatattttcc
    34801 ctaacctgat agtgtatgag aaaagattta acgccgataa actaatcctt tatggttagg
    34861 agaaaaaata atgtggttat tctgggctct taacattgat cattatcaat taaaattaca
    34921 aagcaagaaa ttataagtga actgatattt a
//
LOCUS       DAJSKJ010000020        34600 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000020 DAJSKJ010000000
VERSION     DAJSKJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.020"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            11..2437
                     /locus_tag="ON345_000869"
     CDS             11..2437
                     /locus_tag="ON345_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCR3070973.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMMRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2498..4921
                     /locus_tag="ON345_000870"
     CDS             2498..4921
                     /locus_tag="ON345_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCR3070974.1"
                     /translation="MKIHTTEALMKVEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQSSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLGVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4932..5549
                     /gene="dmsB"
                     /locus_tag="ON345_000871"
     CDS             4932..5549
                     /gene="dmsB"
                     /locus_tag="ON345_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR3070975.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5551..6405
                     /locus_tag="ON345_000872"
     CDS             5551..6405
                     /locus_tag="ON345_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR3070976.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6448..7062
                     /gene="dmsD"
                     /locus_tag="ON345_000873"
     CDS             6448..7062
                     /gene="dmsD"
                     /locus_tag="ON345_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR3070977.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7221..8513
                     /gene="clcB"
                     /locus_tag="ON345_000874"
     CDS             7221..8513
                     /gene="clcB"
                     /locus_tag="ON345_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR3070978.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQLFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8466..9161)
                     /gene="bioD"
                     /locus_tag="ON345_000875"
     CDS             complement(8466..9161)
                     /gene="bioD"
                     /locus_tag="ON345_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR3070979.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRSLLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9286..10506)
                     /gene="mlc"
                     /locus_tag="ON345_000876"
                     /gene_synonym="dgsA"
     CDS             complement(9286..10506)
                     /gene="mlc"
                     /locus_tag="ON345_000876"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR3070980.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVGSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10641..11534)
                     /locus_tag="ON345_000877"
     CDS             complement(10641..11534)
                     /locus_tag="ON345_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3070981.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11641..12894
                     /locus_tag="ON345_000878"
     CDS             11641..12894
                     /locus_tag="ON345_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3070982.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            13291..13626
                     /locus_tag="ON345_000879"
     CDS             13291..13626
                     /locus_tag="ON345_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR3070983.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPERKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            13719..13802
                     /locus_tag="ON345_000880"
     CDS             13719..13802
                     /locus_tag="ON345_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3070984.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13902..14723
                     /locus_tag="ON345_000881"
     CDS             13902..14723
                     /locus_tag="ON345_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR3070985.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLLFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14762..15091)
                     /gene="mdtI"
                     /locus_tag="ON345_000882"
     CDS             complement(14762..15091)
                     /gene="mdtI"
                     /locus_tag="ON345_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR3070986.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15078..15443)
                     /gene="mdtJ"
                     /locus_tag="ON345_000883"
     CDS             complement(15078..15443)
                     /gene="mdtJ"
                     /locus_tag="ON345_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR3070987.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15855..16889
                     /gene="tqsA"
                     /locus_tag="ON345_000884"
     CDS             15855..16889
                     /gene="tqsA"
                     /locus_tag="ON345_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR3070988.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIFTGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16914..18302)
                     /gene="pntB"
                     /locus_tag="ON345_000885"
     CDS             complement(16914..18302)
                     /gene="pntB"
                     /locus_tag="ON345_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR3070989.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18313..19845)
                     /gene="pntA"
                     /locus_tag="ON345_000886"
     CDS             complement(18313..19845)
                     /gene="pntA"
                     /locus_tag="ON345_000886"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR3070990.1"
                     /translation="MRIGIPRERLTNETRVTATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20369..21313
                     /locus_tag="ON345_000887"
     CDS             20369..21313
                     /locus_tag="ON345_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR3070991.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21499..22881
                     /locus_tag="ON345_000888"
     CDS             21499..22881
                     /locus_tag="ON345_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3070992.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22918..23640
                     /gene="folM"
                     /locus_tag="ON345_000889"
     CDS             22918..23640
                     /gene="folM"
                     /locus_tag="ON345_000889"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR3070993.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(23637..23972)
                     /locus_tag="ON345_000890"
     CDS             complement(23637..23972)
                     /locus_tag="ON345_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR3070994.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IFASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24101..24820
                     /gene="rstA"
                     /locus_tag="ON345_000891"
     CDS             24101..24820
                     /gene="rstA"
                     /locus_tag="ON345_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR3070995.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24824..26125
                     /gene="rstB"
                     /locus_tag="ON345_000892"
     CDS             24824..26125
                     /gene="rstB"
                     /locus_tag="ON345_000892"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR3070996.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LIEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26201..27130
                     /gene="tus"
                     /locus_tag="ON345_000893"
     CDS             26201..27130
                     /gene="tus"
                     /locus_tag="ON345_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR3070997.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLDQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27127..28530)
                     /gene="fumC"
                     /locus_tag="ON345_000894"
     CDS             complement(27127..28530)
                     /gene="fumC"
                     /locus_tag="ON345_000894"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR3070998.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(28673..30319)
                     /gene="fumB"
                     /locus_tag="ON345_000895"
     CDS             complement(28673..30319)
                     /gene="fumB"
                     /locus_tag="ON345_000895"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR3070999.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30518..31693
                     /gene="manA"
                     /locus_tag="ON345_000896"
     CDS             30518..31693
                     /gene="manA"
                     /locus_tag="ON345_000896"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR3071000.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGTHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANKLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCQGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            31794..33302
                     /locus_tag="ON345_000897"
     CDS             31794..33302
                     /locus_tag="ON345_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR3071001.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETQLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQQDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGETWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(33347..34594)
                     /gene="uidC"
                     /locus_tag="ON345_000898"
     CDS             complement(33347..34594)
                     /gene="uidC"
                     /locus_tag="ON345_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR3071002.1"
                     /translation="MAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRADKPSAG
                     AGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDISFGFA
                     LGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYWALTP
                     NIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLG
                     ASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQL
                     TWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQ
                     FTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKGREKRDG
                     STLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         8500 a         8929 c         8526 g         8645 t
ORIGIN      
        1 ggagtgagtt atgtccaaaa atgaacgaat ggtgggcatc agccgcagaa cactcgttaa
       61 atctaccgcg ataggttctc tggcgctggc tgcaggcggt ttttctttgc cgtttaccct
      121 gcgcagtgca gcggcaacgg tacaacaggc cagcgaaaaa gtgatctggg gtgcctgttc
      181 cgtcaactgt ggtagccgct gtgcacttcg tctacatgtt aaagataatg aagtgacctg
      241 ggtggaaact gacaataccg gcagcgatga gtacggcaac catcaggtac gcgcctgttt
      301 gcgcggtcgc tccatccgcc ggcgtattaa tcatcccgat cgcttgaatt acccaatgaa
      361 acgcgtgggt acacgcggtg aaggcaaatt cgaacggatt agctgggatg aagccctgga
      421 tactatcgcc agtagcctga aaaaaaccgt cgaacaatat ggcaatgagg ctgtatatat
      481 tcagtactct tcggggattg ttggcggcaa tatgacccgc tcttcgccat cagcctcggc
      541 ggtcaaacgc ctgatgaact gctacggcgg ttcactcaat cagtatggct cctacagcac
      601 cgcgcaaatt tcctgtgcca tgccctacac ctacggcagt aatgatggca acagcaccac
      661 ggatattgaa aacagcaagc tggtcgtgat gtttggtaac aacccggcag aaacccgcat
      721 gagcggcggt ggcatcactt atcttcttga aaaagcgcgc gagaaatcga acgccaaaat
      781 gattgttatc gatccgcgat ataccgatac ggctgctggt cgtgaagatg aatggctccc
      841 tattcgcccg ggcaccgatg ccgcgctggt tgcgggtatt gcctgggtat tgattaacga
      901 aaatctcgtt gatcaacctt ttctcgataa atactgcgtc ggctatgacg aaaaaacctt
      961 acccgcagat gcacccaaaa atggtcacta taaagcctat attcttggtg aaggtgatga
     1021 taacacagcg aaaacgccgc agtgggcttc gcaaattacc ggtatcccgg tagaccgtat
     1081 catcaaactg gcgcgtgaaa ttggcacagc aaaacccgct tatatctgcc agggctgggg
     1141 gccgcaacgc caggcaaacg gcgaactgac tgcacgcgct attgctatgt tacctatttt
     1201 gacgggcaat gtcggcatca gcggcggaaa tagtggcgcg cgtgaatcga cctataccat
     1261 taccatagaa cgcctgccgg tgctggataa tccggtcaaa acgtcaatct cctgcttcag
     1321 ctggacagat gctatcgatc atggtccgca aatgacggca atccgcgacg gcgtccgcgg
     1381 caaagataaa ctggatgtgc ccattaagtt catctggaac tacgcgggaa ataccctcgt
     1441 taatcagcat tctgacatca acaaaacgca tgaaattctg caggacgaat cgaaatgcga
     1501 aatgattgtg gtcatcgaaa actttatgac ctcatcggca aaatatgccg acattctgct
     1561 gccagacctg atgaccgttg agcaggaaga tattattcct aacgactacg ccggtaacat
     1621 gggatatctc attttcctcc agcctgtcac cagcgaaaaa ttcgaacgca aaccgattta
     1681 ctggatcctg agtgaagtcg cgaaacgtct tggacctgac gtctatcaaa agttcacaga
     1741 aggtcgcacg caggaacaat ggttacaaca tctgtacgcc aaaatgcttg ccaaagatcc
     1801 ggcgttaccg tcttacgacg aactgaaaaa aatgggtatc tataagcgta aagatcccaa
     1861 tggccatttt gtcgcctaca aagcatttcg tgacgacccc gaggcaaatc cacttaaaac
     1921 gccttccggt aagattgaaa tttattccag caggctggcg gaaattgccc gtacctggga
     1981 actggaaaaa gatgaagtga taagcccatt gccagtttat gcctcaacct ttgaaggctg
     2041 gaactcccct gaacgtagaa ccttcccact gcaactgttt ggtttccatt acaaatcccg
     2101 tactcactcg acctacggca atattgatct cctgaaggct gcctgccgtc aggaggtgtg
     2161 gatcaaccct atagatgcgc agaaacgtgg gattgccaac ggcgatatga tgcgggtgtt
     2221 taaccatcgt ggcgaagttc ggctaccagc caaagtaaca ccacgtattc tccctggagt
     2281 tagcgctatg ggccagggag cctggcacga ggccaatatg tctggcgata aaatcgacca
     2341 tggcggctgt gtgaatacgc taaccactct gcgcccttca ccactggcga agggaaaccc
     2401 gcagcacact aatctggtcg agatcgaaaa aatataaccc acgacaacca taaattctgg
     2461 catgacattt tgtttgaaaa gcaataagtg agtaatgatg aaaatccata ccacagaggc
     2521 gctcatgaag gttgaaatca gccgtagaag tctgatgaaa acctccgcac ttggcagtct
     2581 tgcgctggca agcagtgctt tcactctgcc attttcccaa atggtccggg cggcagaggc
     2641 tccggtagaa gagaaagcgg tctggagttc ctgcaccgtt aactgcggga gccgctgtct
     2701 gttacgtttg catgtgaaag atgacaccgt gtactgggtg gagtctgata cgacaggtga
     2761 cgacgtctac ggtaatcatc aggttcgagc gtgtttacgc gggcgctcta tccgccgacg
     2821 gatgaatcat cctgataggt tgaaatatcc catgaagcgc gtcggcaagc gcggtgaagg
     2881 taaatttgaa cggataagtt gggacgaagc cctggatacc atcagtgata atcttcggcg
     2941 gatcctgaaa gattacggca atgaggctgt acatgtcctg tacggaacag gcgtagatgg
     3001 cggaaacatc accaactcaa acgtcccgta ccgtctgatg aactcttgcg gtggttttct
     3061 cagtcgctat ggcagctaca gtaccgcaca gatcagtgcc gcaatgagtt atatgttcgg
     3121 tgccaatgat ggcaacagcc cggatgatat cgccaatacg aaactggtcg ttatgttcgg
     3181 aaataacccg gcagaaacgc ggatgagcgg cggtggtgtc acttactacg tcgagcaagc
     3241 ccgcgaacgt tcaaacgcac gcatgatcgt catcgatcca cgttataacg acactgctgc
     3301 cgggcgtgaa gatgaatggc tgcccattcg ccctggcacc gatggcgcac tggcctgtgc
     3361 gattgcctgg gtactgatta ctgaaaacat ggtcgatcag ccatttctcg acaaatattg
     3421 tgttggttac gatgaaaaaa cgctgcccgc caacgcacca cgtaacgcgc attataaagc
     3481 ctatattctg ggcgaagggc ctgacggcat agctaaaacg ccggaatggg cagcaaaaat
     3541 caccagcatc ccggcagaaa aaattatcca gttggcacga gagatcggtt cagcaaaacc
     3601 tgcttatatt tgtcagggtt gggggccaca acgacattcc aacggcgagc aatcgtcccg
     3661 cgctattgcc atgctttccg ttctcaccgg caacgtcggc ataaacggcg gcaactcagg
     3721 cgtacgcgaa ggtagctggg atctgggggt agaatggttc ccgatgctcg agaatcctgt
     3781 taaaacgcag atttccgtct ttacatggac agatgccatc gaccatggta cggaaatgac
     3841 cgcgacccgt gatggtgttc gtggaaaaga aaaactgggt gtccccatca agtttttatg
     3901 gtgctacgcc agtaacacat tgatcaatca acatggcgac atcaatcaca cccatgaggt
     3961 gcttcaggat gacagcaagt gcgagatgat tgttggcatt gaccacttca tgacggcctc
     4021 ggctaagtat tgcgatatcc tgttgcccga cctgatgcca acagagcaag aagaccttat
     4081 ctctcatgaa tctgcaggga atatgggcta tgtgatcctc gcccaacccg caacctcagc
     4141 aaaatttgaa cgcaaaccca tctactggat gctgagtgaa gtcgccaaac gcttaggacc
     4201 agacgtttat caaaccttta ctgaaggtcg cagtcagcat gaatggatca aatatctcca
     4261 tgcgaaaacg aaggaacgta accctgagat gcccgactac gaggagatga aaacgaccgg
     4321 gatctttaag aaaaaatgcc cggaagaaca ctacgtcgct ttccgcgcat tccgtgaaga
     4381 tccacaggca aacccgttga aaacaccttc ggggaaaatc gaaatttatt ctgaacgact
     4441 ggcgaagatt gcagatacct gggaattgaa aaaagatgaa attattcatc cccttcctgc
     4501 gtatacccca ggttttgatg gctgggacga tcccctgcgg aaaacctatc cactgcagtt
     4561 aacgggcttc cattacaaag cgcgtaccca ctccagctac ggcaatattg atgtgttaca
     4621 gcaggcctgc ccacaagagg tgtggatcaa ccccattgat gctcaggcac gcggtatccg
     4681 tcatggcgat accgtgcggg tatttaacaa taatggagaa atgctgattg ccgcaaaagt
     4741 gactccgcgt attctgcctg gcgttaccgc catcgggcaa ggtgcgtggc ttaaagcgga
     4801 tatgtttggt gaccgggtcg atcacggcgg cagtatcaat attctgacct ctcaccgccc
     4861 ttcaccgctg gcaaagggaa acccgtcgca cagcaatctt gtccagatcg aaaaggttta
     4921 aggagtagcc catgaccaca caatatggat tttttatcga ttccagccgc tgtactggct
     4981 gtaaaacctg cgaactggcg tgcaaagatt ttaaagatct tggcccggaa gtcagtttcc
     5041 gccgtattta tgaatacgct ggcggcgact ggcaggaaga taatggcgtc tggcaccaga
     5101 acgtgttcgc ttattatctc tccatctcct gtaaccattg cgatgacccc gcatgtacaa
     5161 aagtctgccc cagcggcgca atgcataagc gtgaagatgg ctttgtggtg gttgatgaag
     5221 atgtttgtat cggctgccgc tactgccaca tggcttgtcc gtacggcgcg ccacagtaca
     5281 atgctgaaaa agggcacatg acgaagtgcg atggttgtta ttcgcgcgtc gccgagggga
     5341 aacaacccat atgtgtcgaa tcctgcccgc tgcgtgcact tgagttcggt ccaattgaag
     5401 agctgcgcca gaagcacggc actctggctg ccgtcgcgcc actgccgcgc gcgcatttca
     5461 caaaacccaa tatcgttatc aaacctaacg ccaacagccg cccgaccggc gataccactg
     5521 gttatctggc taatccggag gaagtgtaaa atgggaaatg gatggcatga gtggccactg
     5581 gttatcttta ccgtattggg tcaatgcgta gtaggcgcgc tgattgttag cggaattggc
     5641 tggttcgccg ccaaaaatga cgcagaccgt cagcgtattg tccgtggcat gtttttcctc
     5701 tggctgttga tgggcatcgg gtttattgcc tctgtcatgc acctgggatc gccactgcga
     5761 gcgtttaact cgctgaatcg aatcggagct tctggtctga gtaacgagat tgctgccggt
     5821 tccatatttt ttgccgttgg cggactatgg tggctggtgg cggtcatcgg taaaatgccg
     5881 caagcgttgg ggaaactctg gttacttgtc agcatggcgc tcggcgtcat tttcgtctgg
     5941 atgatgacct gcgtgtatca aatcgacacc gtgccaacct ggcataacgg ttacactacg
     6001 ctggctttct tcctgacggt attgttgagc ggtccaatac ttgctgccgc aattctgcgg
     6061 gcagctcgcg ttacatttaa taccacacca tttgccatca ttagcgtttt ggcgctgatc
     6121 gcttgtgccg gggtgattgt gctgcaaggt ttgtctctgg cgtctattca ctcatccgtg
     6181 caacaagcca gcgcactggt accagattac gcctccttac aggtctggcg tgtggtattg
     6241 ttatgcgcag gtcttggatg ctggctttgc ccgctcattc gtcgccgtga acctcacgtt
     6301 gccggactta ttctggggct aatcctcata cttggcggtg agatgatcgg gcgtgtgctc
     6361 ttttatggcc tgcacatgac agtaggaatg gctattgcag gttaacaaat tggtgcgggg
     6421 caacccgcac atttcaggat gttaggaatg acccattttt cacagcaaga taatttttct
     6481 gtcgcggcgc gcgtgttggg cgcgctgttt tattacgctc cagagagtgc ggaagccgca
     6541 cctctggttg cggtactcac cagtgacggc tgggaaactc agtggcctct accagaagcg
     6601 tcattagcgc cgctggtgac ggcttttcag acacagtgtg aagagactca cgcccaggcc
     6661 tggcagcgtt tgtttgtcgg cccgtgggca ctgccgtctc cgccgtgggg gtcggtctgg
     6721 ctggatcgcg aatctgtgct gtttggcgat tcaacattgg cacttcgtca gtggatgcgc
     6781 gagaaaggca ttcagtttga aatgaagcaa aacgaaccgg aagatcattt tggttcactg
     6841 ctgttgatgg ctgcgtggtt ggcggagaat ggtcgccaga cagaatgcga agaattgctg
     6901 gcatggcacc tttttccgtg gtcaacacgt tttcttgatg tttttatcga aaaagcagaa
     6961 caccctttct accgtgcact gggtgaactg gctcgcttaa cgctggcgca atggcaatca
     7021 caactgttaa ttcctgtcgc ggttaaaccg ctgtttcgat aggatcaccg taatattgcc
     7081 ggatggtgac gattacgcgc aatccggcaa taataggtta cagtgtcacg tttttttatc
     7141 tcttaaagca cgcactgctt ttgcggctgg cctcttttgc cgcaaaatag tcgcccgtgt
     7201 ttcattgccc atttctgctc atgcatcatc tacacatcta tccggatctg cgcactatgt
     7261 tccgccgtct gcttatcgca acagtcgtcg gtattctcgc ggcctttgcc gttgccgggt
     7321 ttcgtcatgc gatgctgcta ctggagtggt tgttcctcaa taatgactcc ggcagtctgg
     7381 tcaatgcagc gacaaacctt tccccctggc gacggttgct aactccggcg ctcggcggac
     7441 tggcggcggg tttgttgctg atgggctggc agaaatttac ccaacaacgc cctcatgcgc
     7501 cgaccgatta catggaagcg ttgcaaaccg atggacagtt cgattacgca gcaagcctgg
     7561 ttaaatcgct tgcctcgctg ctggtagtaa ccagcggcag tgcaattggt cgcgaaggtg
     7621 cgatgattct tttagctgcc cttgccgcct cctgttttgc ccaacttttt acgccacgcc
     7681 aggagtggaa attatggatc gcctgtgggg ccgcggcggg aatggctgcg gcctatcgtg
     7741 ccccgcttgc tggcagttta tttatcgccg aagtgctgtt tggcactatg atgttggcct
     7801 ctctcggccc ggtgattatt tccgccgtcg tggcattgct ggttagcaat ctgattaatc
     7861 atagcgacgc gttactctac aacgtacaac tctcagtgac ggttcaggct cgtgactatg
     7921 cgctgattat cagtacaggt gtgctggcag gtctgtgcgg accactgttg ttaacgttaa
     7981 tgaacgcctg ccatcgtgga tttgtgagtc tcaaacttgc gccgccctgg caactggcac
     8041 taggcgggtt gatcgtgggt ctgctttccc tgttcacacc tgcagtgtgg ggcaacggct
     8101 atagcaccgt acaatccttt ttaaccgccc caccactgtt aatgatcatt gccgggatct
     8161 tcctttgtaa actgtgtgcc gtgctggcga gtagcggttc cggcgcaccc ggtggggtct
     8221 ttacaccgac gctatttatc ggtcttgcca ttggcatgtt gtatggtcgt agcctgggat
     8281 tatggttccc tgatggcgaa gaaattacac ttttactcgg attgaccggg atggcgacac
     8341 tgttggcggc gaccacgcac gcgccgatta tgtcgacgtt gatgatatgt gaaatgaccg
     8401 gggagtatca gctactcccc ggtttattga ttgcctgcgt aattgcgtcg gtaatttcgc
     8461 ggacgttaca ccgtgactct atctaccgcc agcacactgc gcagcatagc taagcggatg
     8521 tattgcccca gttcacgctg ttcggcgcgc ggcagataag gcagttcacc aatgagcggt
     8581 gccggaagtt ttttacccag cacatcaatg atttccgcat aatgcgccag tcctgggttg
     8641 attcggttag ccacccagcc aatgagcggc agcccgtcgt tggcgatcgc ctgagctgtt
     8701 agcagtgcat ggttaatgca accttcctga ataccgacaa ccatcaacac cggcagttgt
     8761 tcctgcacta cccattcaga gagtggacgc aaatcattca tcagactgcg ccagccgcca
     8821 gtcccttcta ccacgacatg atcgactttt tcggtcaggt ttgccaggcc gtttgaaatg
     8881 agggtgtaat tgattgggca actgtgcgcc acgctacttt cttcttcgct taacgcgata
     8941 ggattaactg cttcataagg cagttcgatg gttgaaacac tctgcaacac cagggcatct
     9001 ttattacgca gcccttcggg tgtctctttg ctccccttcg ctacgggttt atatcccgca
     9061 accgtttttc cctgggaggc taacgcttgt agcaatgagc gggaaaccac cgttttccct
     9121 acagaagtgt ctgtaccggt aataaagaaa cgcttcagca tcactaactc caccgttatg
     9181 cttcacaaat ataaaccagg aaaataatta accttgaaag tctaagttat gctttcctgg
     9241 cccaaattga gatagcgcaa attttggtag aacagttaaa aaatgttaac cctgcaacag
     9301 acgaatcaac aaagaaccgt tatacatcgc gtcttttacc agcgcagccc ctgccatcgt
     9361 accctggtta gaaaactgag tactctcaac gctgatgtgc tgactatacg caggaagggc
     9421 ctgctgacgg atgctgtctg agatgaccgg gaagaggata tctgccgctt tacttaacgg
     9481 tgagccaatc agtatttttt gtgggttaaa taaattcacc atgatggcaa gaatgcgccc
     9541 tacatgcgcg cccaccccgg taatgatgtc ttttgccagc agatcgccgc gcaatgccgc
     9601 ctgacacaat gagcccacgg ttaacggttg tccatgtaac atcgagctca tggattgatt
     9661 aagacgcagc tgtgccagct caagaatact gtccacactg gcgatggttt cgaggcagcc
     9721 gtgattcccg caataacagc gtttcccata cgggtcgacc tgtgtgtggc ctatttccac
     9781 gagactactg ctgcctgcgt gtagcagatg accatcggta atgacgcccg cccccacgtt
     9841 gtgatcgata accacctgaa tcacatcgcg cgccccgcgt gaggcaccaa acaaggcctc
     9901 tgccatcgtc catgcgctaa tatcatgctg aatataaacc ggtacgccgg tatgctgctc
     9961 cagcgcctcg ccgagcggca tctcttttac atcctcgtag aacggcatgc gatgtacaat
    10021 accattttcc gtatcaataa ttcccggcag ggttatggca atcgaagtta gacgctcaag
    10081 ttttttctgg tggcggataa aaaactgatc gatatgggaa atgatacgat ccagcaatgg
    10141 cgagtcatct tttaacgcca gttcctgcga ctcttccacc accagtttgc tgctcagatc
    10201 gcgcagagca aggaaaatct ccccgcgact aatgcgcaga gaaagatagt gccaggcttc
    10261 agtttcaacc accagcccca ccgccggacg gccacggttc cccgcttctt tgatttccag
    10321 ctcttgcacc aggtgtgctt cgagcatctc acggacaatt ttagtgatac tggcaggagc
    10381 cagctgcgcc agacgggaaa gatcgatacg cgagactgga ccaagctgat caatcaggcg
    10441 ataaaccgcg cccgcgttgg tctgctttat ttgatcaatg tgcccaggct ggttttcagc
    10501 aaccaccgca tactccctat attttcgcgc tccgaaataa tctgtaggct atggtgaagc
    10561 acttcaatac gtgtcgtcaa atttttactt aggcatgtga ttaacagcac atttttcggg
    10621 ctttttcgct gaaatttccc tcacctgaga gcattcagca gatgaatacg aaagttacgc
    10681 gcagccggac tttgttcatg atgtttcggc cagaccaacc acatttcaga aaccgcatcc
    10741 tcttcagcaa tcggcaccca gcgcatttcg ttgagctgaa cacgtttaaa tgaagcaggc
    10801 aaaattgaaa cacccagacc ggcggaaacc agaccgatga tggtcattgc ctcgcccacc
    10861 tcctgagtga tgacgggcgt caaatggtaa cgtcgcatca gcccgagaat atcgtcatac
    10921 agccctgtcc cgacgtgcgg atcaaaaaag acgaagggtt ctttcgccag ttcagccagc
    10981 gttacattcg ggttatttgc caggggatga tcgtgcggga tcatcgccat aagcggttca
    11041 tggacgatga ctgcgtgttc aagcgtctcc ggtaacgctg tgttacgcag caatcccata
    11101 tccagcgttc cttcaatgag cggagcgatt tgctcacgag tgttcatttc gcgggtttgt
    11161 aaatggacat cgggataatc acggcgaaac agcgataacg tatcggacac cgcccgaata
    11221 aaaggagcgg acgaagtaaa accaatgcgc aactcccccg cttccccctg atgcagtctt
    11281 tcagcgcgag cggcagcgtc atctaccata gacaggattt gccgactatc tgcaagaaac
    11341 tgttttcctg ctgccgtcag caatacactg cgattagttc gtgccagcag tcgggcacca
    11401 atttgttgct ccagtgcctg aatctgctga cttagcggtg gttgcgaaat attcaggcgg
    11461 gcagcggcgc gcccgaaatg cagctcttcc gcaacagcaa caaagtaacg cagatgacga
    11521 agttcaatat tcatatttaa aacatcttat ttgagattat taatatatta gacagaacaa
    11581 ttcgattttc ctaacctatg tataagcctg atccacaggc atatttagca aggatttcaa
    11641 gtgagccgta ctacaactgt tgatggcgct ccggcaagcg acactgacaa gcaaagcatt
    11701 tctcagccaa atcaatttat taaacgcggt acgccgcaat ttatgcgcgt caccctggcg
    11761 ctgttctctg ccggactggc aacatttgca cttctctatt gtgtgcagcc tatccttccg
    11821 gtgctttcgc aggagtttgg cttaacgccc gcgaacagta gtatttcact gtccatttcc
    11881 acggcgatgt tggctattgg tttgctgttt actggccccc tatccgatgc cattggtcgc
    11941 aaaccagtga tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg
    12001 atgaccagct ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc
    12061 gtggcagctg ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt
    12121 tcgatggggt tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagt
    12181 ggtgtcttca cggacttttt caactggcga attgctctgg cggcaatcgg ttgtttcgcg
    12241 ctggcctcgg cgttgatgtt ctggaaaatc cttccggaat cacgccattt tcgcccgact
    12301 tcgctgcgcc ctaagacgtt atttatcaac tttcgtctgc actggcgtga ccggggatta
    12361 ccgttattgt tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac
    12421 atcggctatc ggttgatgct ttccccctgg catgtcagtc aggccgtggt tggcttatta
    12481 tcgctggctt atttgaccgg tacatggagc tcacccaaag ccggaaccat gaccacccgc
    12541 tacgggcgcg gtccggtgat gttgttttcg acgggggtta tgctgtttgg tttactgatg
    12601 accttattca gctcgctgtg gctgatcttt gccggaatgt tactcttctc agcaggattc
    12661 ttcgcagccc actcagttgc cagcagctgg attggccccc gcgcaaaacg cgctaaaggc
    12721 caggcctcct cgctgtatct gttcagttac tatctggggt cgagtattgc cgggacgctg
    12781 ggtggtgttt tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg
    12841 ctggtcattg ctctgctggt cgggacgcgt ttgcatcgtc gtctgcacgc ctgaaaaata
    12901 agcccggact gcggtaacta cccgtccgga cttattgctt gctcaaacca acgttaatag
    12961 ccatcctaaa atagacgaag cgccagccaa ttcccgcagc gcgtctagcg tcatcaggat
    13021 tataagtacc caaataaacg gattcatttt gctgtgtgtc atttattact gatgcgcagt
    13081 tattctaccg ctttgtaagt agtaaaatag ttaacccgat caagactact attattggta
    13141 gctaaatttc ccttaagtca caatacgtta ttatcaatgc tgtaatttat tcagcgtttg
    13201 tacatatcgt tacacgctga aaccaaccac tcacggaagt ctgccattcc cagggatata
    13261 gttatttcaa cggccccgca gtggggttaa atgaaaaaac aaattgaggg tatgacaatg
    13321 aaaaaagtat tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct
    13381 gcagagactg cgaccacacc tgctccgact gcgacgacca ccaaagcagc gccggcgaaa
    13441 acgacacatc ataaaaaaca gcataaagca gcacctgccc agaaagcgca ggcggctaaa
    13501 aagcatcata aaaatacgaa agctgaacag aaagcccctg aacgaaaagc gcaggcagcg
    13561 aagaaacacg ccgggaaaca cagccatcag caaccggcaa aacctgctgc acaacccgca
    13621 gcgtaagttt acaacagtaa tgctggcgcg ccctctcgcg cctgaaaatt acggtgctaa
    13681 gcgggtaacg tttagcaccg cctttagcgg agggtagtat ggtgggccgt tatcgctttg
    13741 agttcattct tatcatcctt attttatgcg cactgattgc cgcccgtttt tatctttcct
    13801 gactgtagtt atctgatttt actcccactt tcatcccgtc ccgtctatag tatttatgag
    13861 ggtttgcttt taataatcat aattacccac cagagtgtga tatgcgtaca accattgctg
    13921 tagtgttggg tgcaattagt ttgacgtctg cttttgtgtt tgcagataaa ccagacgttg
    13981 ccaaatcggc aaacgatgag gtcagcaccc tgctttttgg tcatgatgat cgtgtgccag
    14041 tgaatgacac gacccaatca ccgtgggatg cggttgggca actggaaacg gccagcggca
    14101 atttatgtac ggcgacgctg attgcaccca atctggcatt aacggcagga cactgtttat
    14161 tgacgccgcc taagggtaaa gcggataagg cggtagcgct gcgttttgtg tcaaataaag
    14221 gtctttggcg ctatgacatc cacgacatag aaggccgcgt tgatccgaca ctgggaaagc
    14281 ggttaaaagc agatggggat ggttggattg ttcctcccgc agctgcacca tgggacttcg
    14341 gcttgattgt gctgcgtaat cccccttctg gcattacacc gttgccgtta tttgagggag
    14401 ataaagccgc gcttactgcc gcattaaaat cggctggtcg taaagtgact caggcgggat
    14461 atcctgaaga tcatctcgat acgttataca gtcatcaaaa ctgtgaagtg actggctggg
    14521 cgcaaacgtc ggtgatgtca catcagtgcg ataccttgcc gggtgacagc ggttcgcctc
    14581 tgatgttgca taccgatgac ggctggcaat taattggggt gcaaagttcg gctcctgccg
    14641 cgaaagatcg ctggcgcgcc gataaccggg ccatttctgt taccggtttt cgcgacaagc
    14701 tggatcaact gtcgcaaaaa taatgttcaa acgctgcccg acagtgcggg cagcgtgttc
    14761 atcaggcaag tttcaccatg atcattccag ccaacagcaa gaccaggcca atccagcctt
    14821 tacgatttaa ccgttgacca aacaagatcc aacctgcggc taacgtggcg gcaataccaa
    14881 acccgcccca caatgcataa gcgacagaca agtcgatccc ttttacggcc tgagaaagcg
    14941 cactaaaggc agccagcacc gccgccaggg agagcaagcc aaatattttg cgacgaaagc
    15001 cgtcagaaaa tttcaaaaag acgttagcaa cgatttccag cacgattgcc aatgccagcc
    15061 aggcggcgtg aacccattca aactgcgcca tggttcacct ccagttcagg tttacgcgct
    15121 ttacgggtac ctgatttgat caacacaatc ccggcgacca gggtggttaa cccggcaatt
    15181 ttcatcagcg ataaactttc gtcgaataac aaaacgctaa acaaggtaat aaataaaata
    15241 ccgatacctt cccacagcgc ataagctacg cctaaggcga tctttttaac ggcgaaagag
    15301 agaaatatat acgacagaga aatcatcacc agcattaaaa taaagccgcc atttccctca
    15361 ctgacgctcg cccatttcat tgacagcgta ccggtaattt ctgtagcaat agccaggcct
    15421 aataaaatcc aataaatata cattgtcctt ctcctgcaag agaattattt taattttcgc
    15481 ttaattcagc gaaaccaaag taaagtggtc aagccccgcg catagggcaa gcttaggcag
    15541 aagaaaggac taaagcgcgt tgcgccagtg ctgctcacct acgagcaaga tagtggatga
    15601 ggtacgaaga tgggtaaatg ttgaaaataa cgtcctgaac aaattgtcca taatattaca
    15661 attatccgca gtgttgcttc tcgtcatcgc ggatgataat tgtcctcggt agttgaacac
    15721 gcctgatttg tatcatagct taagaattaa ctcaaaatat tttcacttct ttacctgagc
    15781 ggtttgattt tcgttatgat gacggagcga aaaagacatt attattagca aaggaagaaa
    15841 aaacggggac aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta
    15901 tgttgggaat gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc
    15961 cgtttattct cgcattattt attgctgtta ttcttaaccc gctggtgcaa cacatggtcc
    16021 gctggcgagt gccgcgtgta ctggcggtgt cgattttgat gaccatcatc gtgatggcga
    16081 tggtgttgct attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc
    16141 aatatcgcaa ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag
    16201 ggattgacgt ctcagttgac cagctggcgc attacattga tccgaacgcg gcgatgacgt
    16261 tgctcaccaa cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc
    16321 tgacggtgct gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga
    16381 tggcgcgtcc ggttgaaggg atggcggcga ttcaacgtgc aattgacagt gtttctcatt
    16441 atctggtgct gaaaacagcc atcagcatct tcaccggcct ggtcgcctgg gcgatgctcg
    16501 ccgcactcga tgttcgcttc gcttttgtct ggggattgct ggcctttgcg cttaattaca
    16561 tccctaatat tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta
    16621 atggcttcta cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg
    16681 gcaatattct ggagccgcgc atcatgggac gtgggctggg gctttccaca ttggtggtat
    16741 ttttgtcgtt gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc
    16801 cgttaacaat tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg
    16861 ttctgttaag cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga
    16921 gctttcagga ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc
    16981 ttgaagaaca gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca
    17041 atcacgttct gcgctttcca cacttccagc acaggcatac cagcaatagg actcttcgga
    17101 tcatcctgcg ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta
    17161 tcagcaaagt catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct
    17221 tcagccagca atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa
    17281 cgcacgttga taccacgagc gcgcagtttc tcggtaattt cagcgacagg atattgcgcc
    17341 tgcgcgactg ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt
    17401 tccgctgtct cttctgcggt gatttcgcgg tgctcgccca cttcctgatc atcgccagta
    17461 gaagagccgt cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc
    17521 ttacacataa tgtaagaaag gatagcgccc gaagaaccga ccagcgcacc ggtcacaatc
    17581 agcaggtcgt tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc
    17641 agcatcgaca ccaccactgg catatctgca ccaccgatgg aggcgactaa atgccagccg
    17701 aataccagcg caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctatcc
    17761 gtgcgaacaa atacaatcag cagcaggaag gaaacgacca gagccgccag gttcattttg
    17821 tgacggtttg gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc
    17881 accaccgaac ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg
    17941 tgaatattga ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca
    18001 accagcactg ccgccagacc cacgaagcta tgcaggatcg ccaccagttc tggcatttcg
    18061 gtcatttcaa ctttcttcgc cagacggata ccaattgccc caccaatgac catcgccagc
    18121 aagatccagc caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc
    18181 atcccggcga taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc
    18241 agactgaaga taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac
    18301 atatgttacc ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc
    18361 accgaaaata ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc
    18421 gccctggcca atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtgac
    18481 cgacatcaac ggtgtatgca gcgcatgcga tacattccac accacgtaat aaccgacaac
    18541 gcaggccagc gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgccag
    18601 ccaaccgaaa aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc
    18661 acatttttcc tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga
    18721 tacctgaatc ggcggcgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg
    18781 aatcaccaca tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt
    18841 cagcagatta acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg
    18901 aagatcggta taaccaatca ctttgacacc attttccgta gtgaagattt cacccggcac
    18961 ggtgtattca cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt
    19021 catggagtca accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc
    19081 ggtggtgaca atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt
    19141 aatgaacgcg tccgacatca ctttggcata gccatcgccg ctgcccgctt cctctttaaa
    19201 atccagctcg aggaattccg cgcccatact ttgaacttgt tctttcactt ccggacgggt
    19261 gtcgaatgca cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccagacctgc
    19321 aacacccgca ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtaatttg
    19381 cccggtaaag aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat
    19441 gttcgccatc gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagaatc
    19501 catcgccatc acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc
    19561 aggccagata aaactcacca gcgttgtccc aggattcagt aacgcaattt catcatctaa
    19621 cggcgcattg accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc
    19681 agcgcccgct tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc
    19741 taccgcgacg gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgtaac
    19801 acgggtttca ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat
    19861 cggttttatt gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc
    19921 taaatgttac tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat
    19981 gcctgtgatc tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt
    20041 cagccgtgat agtgggataa acaccttaga acgccggata aagactgata attgtcttcg
    20101 acggtcgggt aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact
    20161 tatacaattc agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac
    20221 ggtcatttga gcaggattaa aattggctta aggaatgtga tatgaaaaat gacgcagaca
    20281 gttacaccgt ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct
    20341 ggtttgcgca aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc
    20401 actgctttct gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc
    20461 ggcagcggtt aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga
    20521 agcggtgaaa gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt
    20581 cgacacttct gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc
    20641 cgatgctgaa aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc
    20701 gaaagatcag gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg
    20761 tagccagcca gaagtcaatg atgccatcac caaagcagca aaagcgaaag gtgcctactc
    20821 tttctacatt gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt
    20881 catctataaa aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga
    20941 ttccgaagca ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat
    21001 cccaggtgtt gcgactaccg catcaccaag ttctgaagtc ggtcgtttct ttgaaaccca
    21061 gtcatcaaaa ggcggacgtt acactgtcac gcttcctgac ggcactaaag tcgaagaatt
    21121 gaacaaagcg acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta
    21181 cggcaacatg accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa
    21241 gtattaccac atcacccgcc agtggcagga acgtggtaat aacctgaccg tcagcgcaga
    21301 tctgtataaa taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt
    21361 ttagtcacaa atttccacgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg
    21421 cttcagtggc aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt
    21481 tttaatcact ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta
    21541 ttaagctcaa tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc
    21601 agcccggcag cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc
    21661 tttgccatgc tgatcctcac gcgcattcgt cccgaacttg acggcggtat ctttacctat
    21721 gcccgcgaag ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc
    21781 gcagtcatcg ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg
    21841 gacacgccgg aattgcgcct gttcggcgat ggtaatacct ggcaatcgat agtcggtgca
    21901 tcggctttat tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc
    21961 attaacctgg tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg
    22021 atgatgatgt tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta
    22081 cccgtgtggg aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg
    22141 gaaggtgccg tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca
    22201 ctgctggcgg ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggc
    22261 gtggtggcac gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg
    22321 gaaatgatgg gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc
    22381 ggcgcgtact taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat
    22441 aaagcattcc cacgcatttt tgcgcgccag aacgcacaag ctgcgccatc cgcctcgctg
    22501 tggttgacca acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat
    22561 tacaacacgt tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc
    22621 gcgttcttgc tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc
    22681 atttatggct tatggttatt gtatgcctct ggtccaatgc acttgctgtt atccgttgtt
    22741 ctgtatgcgc cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat
    22801 gtactgaacc gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg
    22861 acctggatgc tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg
    22921 ggtaaagccc agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc
    22981 ctcgcctggc atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacgcactat
    23041 ccagccattg atggactgat taaggcaggt gcgcagtgta ttcaggctga tttttcgacc
    23101 aacgacggtg tgatggcgtt tgccgatgaa gtactaaaaa gcacccatgg tctgcgtgct
    23161 attttgcata acgccagtgc gtggatggcg gaaaaaccgg gtgcgccact ggccgacgta
    23221 ctggcttgca tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa
    23281 agattactgc gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg
    23341 gtggagcgcg gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat
    23401 atgacccgct cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca
    23461 tcgctgatcc tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa
    23521 tcactgatga aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt
    23581 accagctgct ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa
    23641 tgcagcttta tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa
    23701 ccaacaaaag cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa
    23761 acaaaatagg gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg
    23821 ccgcgttcgc tggcaaaaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc
    23881 agcccggcga tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc
    23941 gcaccgagcg cggctttgat taccagcccc atctttttac cttaacactt ccataacaag
    24001 tcatcagtag aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag
    24061 cgcggtgtat tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt
    24121 ggaagatgat gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca
    24181 ggttaccgta gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga
    24241 tttggtgtta ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt
    24301 acgcgcaaag tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca
    24361 catcctggca ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt
    24421 tttgctagcg cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg
    24481 tcttcaggaa acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga
    24541 ccccatcaac cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt
    24601 cgaattattg tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct
    24661 gaaaaattta cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc
    24721 gcggttaaga aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg
    24781 taacaaaggc tatctttttg cgcctcatgc atgggaataa gcgatgaaaa aactgtttat
    24841 ccagttttac ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt
    24901 gtacaaattt accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc
    24961 gctgtatctg atgcgtagcg aattgcgtga gatcccccca cacgattggg gtaagacgct
    25021 gaaggagatg gatttaaatc tctctttcga tctgcgtgtc gagccactga gtaaatacca
    25081 tcttgatgat atttccatgc accgactgcg tggcggcgaa attgtcgccc tggacgatca
    25141 gtacacgttt ttgcagcgta tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc
    25201 ttatctttat tatctccatc agatgcgatt gctggatatc gccctgatcg cttttattgc
    25261 tatttccctc gcctttccgg tgtttatctg gatgcgtccg cactggcagg atatgttaaa
    25321 actggaagca gcggcgcaac gatttggcga tgggcatctc aatgagcgta tccactttga
    25381 tgagggttcg agctttgaac gacttggtat cgcatttaac cagatggcgg acaatatcaa
    25441 cgccttgatt gccagcaaaa aacagcttat tgacggtatc gctcacgaac tgcgaacacc
    25501 gttagtgcgc ctgcgttatc gactggagat gagcgataac ctgagcgccg ccgaatccca
    25561 ggcgttgaat cgtgatatca gtcaacttga agctttaatt gaagagctgc tgacttatgc
    25621 ccgactcgat cgcccacaaa acgagcttca tcttatcgaa ccagacctgc cgttgtggct
    25681 gtcaacgcat ctggcagata ttcaggcagt aacgcccgat aaaacggtac ggataaaaac
    25741 gctcgtgcaa ggccattatg cggcgttgga tatgcgctta atggagcgcg tgctggataa
    25801 tttgctcaat aacgccctgc ggtactgcca ttcaacggtt gaaaccagcc tgctactgtc
    25861 ggggaataga gcgacattaa ttgttgagga tgatggccca gggattgccc cagaaaaccg
    25921 cgaacatatc tttgaacctt ttgttcgcct cgatcccagc cgggatcgct caaccggcgg
    25981 ctgcgggctg gggctggcaa ttgtccactc tatagcactg gcaatgggcg gtacggttaa
    26041 ttgtgacacc agcgaactgg gtggtgctcg cttctcgttt agctggccgt tatggcataa
    26101 catcccgcaa tttacctctg cctgacacta cgcgcacgat ggtcaagtca ccaccattgt
    26161 gctataaatt aaggatgttg taactaaagt ggttaatatt atggcgcgtt acgatctcgt
    26221 agaccgactc aacactacct ttcgccagat ggaacaagag ctggctgcat ttgccgctca
    26281 tcttgatcaa cacaagctat tggttgcccg cgtgttctct ttgccggagg taaaaaaaga
    26341 ggatgagcat aatccgctta atcgtattga ggtgaaacaa catctcggca acgacgcgca
    26401 gtcgctggcg ttgcgtcatt tccgccattt atttattcaa caacagtccg aaaatcgcag
    26461 cagcaaagcc gctgtccgtc tgcctggcgt gttgtgttac caggtcgata acctttcgca
    26521 agcagcgttg gtcagtcata ttcagcacat caataaactc aagaccacgt tcgagcatat
    26581 cgtcacggtt gagtcagaac tccccaccgc ggcacgtttt gaatgggtgc atcgtcattt
    26641 gccggggctg atcaccctta atgcttaccg ctcgctcacc gttctgcacg accccgccac
    26701 tttacgtttt ggctgggcta ataaacatat cattaaaaat ttgcatcgtg atgaagtcct
    26761 ggcacagctg gaaaaaagcc tgaaatcacc acgcagtgtc gcaccgtgga cgcgcgagga
    26821 gtggcaaaga aaactggagc gagagtatca ggatatcgct gccctgccac agaacgcgaa
    26881 gttaaaaatc aaacgtccgg tgaaggtgca gccgattgcc cgcgtctggt acaaaggaga
    26941 tcaaaaacaa gtgcaacacg cctgccctac accgctgatt gcactgatta atcgggataa
    27001 tggtgcgggc gtgccggacg ttggtgagtt gttaaattac gatgccgaca atgtgcagca
    27061 ccgttataaa ccacaggcgc agccgcttcg tttgatcatt ccacggctgc acctgtatgt
    27121 tgcagattaa cgcccggctt tcatactgcc gaccatctgt tctggccgta cccagctgtc
    27181 aaactcggct tcgctaagat accccagcgc aagggccgca gcttttaagg tcagcccttc
    27241 tttatgcgct tttttggcga tctccgcggc tttgtcataa ccaatgtggg tgttaagcgc
    27301 agtcaccagc atcagcgatt cattgagtaa ttgattgatt cgctcacgat tcggttcaat
    27361 acccactgcg cagtgtttgt taaaactttc catgccatct gccagcaagc gcaccgattg
    27421 caggaaatta tggatcacca tcggacggaa gacgttcagt tcaaagttac cggaagcgcc
    27481 acccatgttg atcgccacgt cgttccccat tacctgacag cagagcatgg ttaatgcttc
    27541 acactgggtt gggttcactt tccccggcat gattgagctg cccggctcat tttccgggat
    27601 tgagatttca ccaattccgc agcgcgggcc agaggccagc cagcggacat cattggcgat
    27661 tttcatcagt gacacagcca accctttcaa cgcgccgtgc gcctgaacca gggcatcaca
    27721 ggtcgccagc gcttcaaatt tgttcggcgc ggtaacaaac ggtgcacagg taatgactgc
    27781 cagttcatct gctacgcgac gcgcatactc cggatgggta tttagtccag tacccaccgc
    27841 tgtaccgcca agagccagtt ccgctacgtg aggcaggctg tattcgatat gtttgagatt
    27901 atgctcgagc atcgctaccc agccggaaat ctcctgcccc agtgttagcg gcgtggcgtc
    27961 ctgcaagtgg gttcgaccga tttttacgat atcggcaaat gcacgcgatt tttcactcag
    28021 cgtctgtgtc agggttttaa gctgcggaat gagttgcttg cgcagcgcca gcagcgccgc
    28081 aacgtgcatc gccgtcggaa agacatcgtt ggaactttgg cttttgttca cgtcgtcgtt
    28141 agggtgaact ttacgttcca tcccgcgcac gccgccgagt aattcactgg cccggttagc
    28201 cagcacttcg ttcatgttca tgttactttg cgtgccggag ccggtctgcc agatagccag
    28261 cgggaattcg tcgtcatgct gtcctgccag tacttcatcc gccgcctgcc gaatggcgct
    28321 cgctttctct tcagacaaca agcctaaatc ttcattaact tttgccgctg cgcgcttggt
    28381 tagcgccagc gcatgaatca gtgaggtggg cattttctcc gtcgaaatgc ggaaatgctc
    28441 cagcgagcgt tgagtttgtg cgccccacag cttatctgcc gggacatcaa tcgcccccat
    28501 cgaatctttt tcgctgcgta ctgtattcat ggcctgctcc tcacctgttt aattttttct
    28561 ttctgttttg ctttcgttaa gcaactttta gctcacttat tatttactat ttgataacaa
    28621 atgtttggtc tttcgtgcca tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca
    28681 cacagcgagt gcactgtgtg agttgtatct gctggaagaa gtcatttcct ttatcatcca
    28741 caaggataaa cgccgggaaa tcttccactt caattttcca gatggcttcc attcccagtt
    28801 ccggatattc aacacattcc aggctcttaa tacttccctg cgccaataca gcggccggac
    28861 caccgatact gccaaggtag aagccgccgt gttttttaca ggcatccgtc acctgctggc
    28921 tgcggttgcc tttcgccagc atgatcatac ttccgccctg cgcttgcagt tgatcgacat
    28981 aagaatccat ccgtccggcg gtcgttgggc caagagaacc ggaggcataa ccttccggcg
    29041 ttttggccgg acccgcgtag taaatcggat gatctttaat gtactgcggc agcccttcac
    29101 cgttatccat ccgctctttc agtttggcgt gagcaatatc acgaccgacg ataatcgtgc
    29161 cgttaagcga taagcgtgta gaaacgggat actgcgacaa ctgtgcgagg atctctttca
    29221 tcggacggtt aaggtcaacg cgcaccgctt cgccttctcc cgctttacgc agctcttcag
    29281 ggatatattt acctggatta tgttccagtt tttcgatcca gatcccctga cggttgatct
    29341 tcgctttgat attacggtca gcagagcagg aaacacccat accaaccgga caggatgcgc
    29401 cgtgacgtgg caggcgaatc acgcgtatgt cgtgagcgaa gtatttacca ccaaactgcg
    29461 cacccagacc gagattttgc gcttcgatca gcaattcttt ttccagttcc acatcgcgga
    29521 atgcctgacc gtgctcattc ccttccgttg gcagttcgtc atagtattta gcggaagcca
    29581 gtttcactgt tttaaggtta gtttctgcag aagttccacc aattacgaac gcaatatgat
    29641 acggaggaca ggccgccgta cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt
    29701 tccccggcgt tagtaacgct ttggtttcct gatagagata cgtcttgttt gccgaaccac
    29761 cacctttggc gatacagagg aatttgtact cgtcgccatc aacggcataa agatcgatct
    29821 gcgctggcag attggtgccg gtattcacct ctttatacat atccagcggc gcgttttgcg
    29881 agtagcgcaa attatcttcg atataagtgt tatagacacc gcgcgccagc gccgcttcat
    29941 cacagccacc ggtccataca cgctgccctt ttttaccaac aataatcgca gtgccggtat
    30001 cctgacaggt tggcagaacg cctttcgccg cgatgtcgga gttacgcagg aattgcagcg
    30061 ccacatattt atcattttcg ctggcctccg ggtcacgcag aatgtcggcc acctgttgtt
    30121 ggtgagccgg acgcagcata aacgacgcat catgaaacgc ctgacgcgcc aacagagtta
    30181 acgcttcggg tgcgactttc aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt
    30241 cgctggttag caggtaatac tcagtatcat cttttttgag tggaaaagga gcctgataat
    30301 gaaagggttt gtttgacatt gttctctcac ttactgcctg gtttggttat gctctgggcg
    30361 ggtgttccgt tgccctgtta aaagcgagta acaatatcct acacactttt ttaacaaaaa
    30421 ctgagactag tacgactttt tgcggctcca ggttacttcc cgtaggattc ttgctttaat
    30481 agtgggatta atttccacat taaaacaggg attgatcatg caaaaactca ttaactcagt
    30541 gcaaaactat gcctggggca gcaaaacggc gttgactgaa ctttatggta tggaaaatcc
    30601 gtccagccag ccgatggccg agctgtggat gggcacacat ccgaaaagca gttcacgagt
    30661 gcagaatgcc gccggagata tcgtttcact gcgtgatgtg attgagagtg ataaatcgac
    30721 tctgctcgga gaggccgttg ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt
    30781 atgcgcagca cagccgctct ccattcaggt tcatccaaac aaacgcaatt ctgaaatcgg
    30841 ttttgccaaa gaaaatgccg caggtatccc gatggatgcc gccgagcgta actataaaga
    30901 tcctaaccac aagccggagc tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt
    30961 tcgtgagttt tccgagattg tctccctact ccagccagtc gcaggtgcac atccggcaat
    31021 tgctcacttt ttacaacagc ctaatgccga acgtttaagc gaactgttcg ccagcctgtt
    31081 gaatatgcag ggtgaagaaa aatcccgcgc gctggcgatt ttaaaatcgg ccctcgatag
    31141 ccagcagggt gaaccgtggc aaacgattcg tttaatttct gaattttacc cggaagacag
    31201 cggtctgttc tccccgctat tgctgaatgt ggtgaaattg aatcctggcg aagcgatgtt
    31261 cctgttcgct gaaacaccgc acgcttacct gcaaggcgtg gcgctggaag tgatggcgaa
    31321 ctccgataac gtgctgcgtg cgggtctgac gcctaagtac attgatattc cggaactggt
    31381 tgccaatgtg aaattcgaag ccaaaccggc taacaagttg ttgacccagc cggtgaaaca
    31441 aggtgcagaa ctggacttcc cgattcctgt ggatgatttt gccttctcac tgcatgacct
    31501 tagtgataaa gaaaccacca ttagccagca gagtgccgcc attttgttct gcgtcgaagg
    31561 cgatgcaacg ctgtgtcaag gttctcagca attacagctt aaaccgggtg aatcagcgtt
    31621 tattgccgcc aacgaatcac cggtgactgt caaaggccac ggccgtttag cgcgtgttta
    31681 caacaagctg taagagctta ctgaaaaaat taacatctct tgctaagctt attaaaggct
    31741 tataacacct tcaggcggcc agtccgcctg atttcatttt atggataatc attatgaata
    31801 aatcgctggt agcggtaggc gtcattgttg cgctaggcgt agtctggaca ggcggcgcat
    31861 ggtatacagg caagaagatt gaaacccaac tcgaagagat ggtcgcgcag gcgaacgcgc
    31921 aactcaaact gacagctcct gaatccaacc tggaagtgag ttatcaaaac tatcatcgcg
    31981 gcgtattcag cagccagttg caactgttgg tgaaacccat tgccgggaaa gaaaatccgt
    32041 ggattaaaag cggtcagagc gtcatcttca acgaatcggt tgatcatggt cccttcccgc
    32101 ttgcccagct taaaaaactg aacctgatcc cgtcgatggc atcaattcaa accacgctgg
    32161 ttaataacga agtaagcaaa ccactgtttg atatggcaaa aggtgaaacg ccttttgaga
    32221 ttaactcgcg cattggttac agcggtgatt ccagttccga tatttcgctc aagccactga
    32281 attacgagca acaggatgaa aaagtcgcct ttagcggcgg cgagttccag ttaaatgctg
    32341 acagagacgg caaagctatc tccctttccg gggaggcgca aagtggtcgg atagacgcgg
    32401 ttaacgaata caaccagaaa gtgcagttga cctttaataa tctgaaaacc gacggttcca
    32461 gcacgctggc aagttttggt gagcgcgtag gaaaccaaaa actgtcactg gaaaaaatga
    32521 ccatttcagt ggaaggcaaa gaactggcac tgctggaagg catggagatc agcggtaaat
    32581 cggatctggt caatgacggt aaaacgatca atagccaact ggattactcg ctaaacagcc
    32641 tgaaggtaca gaatcaggat ctgggcagcg gcaagctgac tttaaaagtc ggccagattg
    32701 atggtgaaac ctggcatcag tttagccagc aatataacgc gcaaactcag gcgctgctgg
    32761 cacagccaga aattgccaac aatcccgaac tttatcagga gaaagtgacg gaagccttct
    32821 ttagcgccct gccgctgatg ttgaaaggcg atccggtgat tactatcgca ccgctaagct
    32881 ggaaaaacag tcagggtgaa agtgcgctga atctgtcgct gttcctgaaa gatccggcaa
    32941 cgactaaaga agcgccgcaa acgctggcgc aggaagtaga tcgttcggtt aaatctctgg
    33001 atgcgaaact gaccattccg gtggatatgg caactgagtt tatgactcag gtagcgaagc
    33061 tggaaggtta tcaggaagat caagcgaaaa aactggcgaa acagcaagtt gaaggtgcat
    33121 cagcaatggg gcagatgttc cgtctgacca ccttgcagga caataccatc accaccagcc
    33181 tgcaatatac taacggtcag ataacgttaa acgggcagaa aatgccactg gaagatttcg
    33241 ttggtatgtt tgcaatgccg gcattaaatg ttccggtcgt acccgctatt ccgcagcagt
    33301 aattcacagc caaaacgccg gagatttttc tctccggcgt tattttttac ttcagcataa
    33361 agtcatactg aatactcatt ttcacatccc agtaattcag gcgatcgccc agcgtactgc
    33421 catcgcgctt ttcccgccct ttatcggcag caagaaagaa attcatccct tgtaacggcc
    33481 cttcgctgac ccgatagttc ataccaccta agatacccgt gccttcaatc cgcacatctc
    33541 gtttactgga ttcatagccg cgagtcacaa tcggtgcaaa tgtcagcgta aattgcggcg
    33601 ttaaacgata gttgacgccc aattgccagg actgcattcc ttcacggtta cgatcaagac
    33661 ttaatgaaaa gggatagcca atgtcggtat ccaccacggc accattcatt gcatggcgca
    33721 aataaccacc actgccaaat acgcttcccc acggcgcatt ccaggtcaat tgtccactaa
    33781 ccaacgccgt ttcattgggc tggctggtat tacgacttaa accttcgagc tgcgcgtaaa
    33841 agccgagcaa ctcccctttc aacgtataat tcgggtcgag cggtatgctt ttctcgagaa
    33901 tactttgtat ctgattggaa acatctttct gtaccgatgc ccccagagaa tagcgactgg
    33961 tatcatcatg ccaactggcg gctaaaaaag aacgtgcgcg atgtttatcg tattttcccg
    34021 tctggctgtt atacacccct tcatcacgaa taccggttgt tgaatcagtc cataaaaata
    34081 ctcgtgaacg ccacattccc cacagatcaa tatttggtgt taaagcccaa taacctaatg
    34141 ctccttcaga aactgtcggt aatgtacgtt gcgaactgcc agccattaac ggaatacgat
    34201 aaggtaagct accataacta taatccgtcc cgaagcgacc taatttaagc aggctatttt
    34261 cactgggttt tatttttact gcccccaggg caaagccaaa acttatatca ccatcaagat
    34321 accaccgggt actcatatca gcacgagcac ctaatttata aacataatat gccccgcctt
    34381 caacgccaat aatattggaa taataaccac tattgaaatc gagcaatcct ccctgtaccc
    34441 atgcgtaaat atctcttccc gcgccagcac tgggcttatc cgccctgcgc aattcgtttt
    34501 tcagtctgat gcgtagtccc gcttcatcgt cagccagttg cgccgcataa gcagaggtaa
    34561 ggccagaggc agccgtcagg caaataacgg ccatggccac
//
LOCUS       DAJSKJ010000021        32988 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000021 DAJSKJ010000000
VERSION     DAJSKJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.021"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(19..1878)
                     /gene="gss"
                     /locus_tag="ON345_000899"
     CDS             complement(19..1878)
                     /gene="gss"
                     /locus_tag="ON345_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR3071003.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2083..2949
                     /gene="yghU"
                     /locus_tag="ON345_000900"
     CDS             2083..2949
                     /gene="yghU"
                     /locus_tag="ON345_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR3071004.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3072..3320)
                     /gene="hybG"
                     /locus_tag="ON345_000901"
     CDS             complement(3072..3320)
                     /gene="hybG"
                     /locus_tag="ON345_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR3071005.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3333..3674)
                     /gene="hypA"
                     /locus_tag="ON345_000902"
     CDS             complement(3333..3674)
                     /gene="hypA"
                     /locus_tag="ON345_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR3071006.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3667..4155)
                     /gene="hybE"
                     /locus_tag="ON345_000903"
     CDS             complement(3667..4155)
                     /gene="hybE"
                     /locus_tag="ON345_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR3071007.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4148..4642)
                     /locus_tag="ON345_000904"
     CDS             complement(4148..4642)
                     /locus_tag="ON345_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR3071008.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4642..6345)
                     /gene="hybC"
                     /locus_tag="ON345_000905"
     CDS             complement(4642..6345)
                     /gene="hybC"
                     /locus_tag="ON345_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR3071009.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6342..7520)
                     /gene="hybB"
                     /locus_tag="ON345_000906"
     CDS             complement(6342..7520)
                     /gene="hybB"
                     /locus_tag="ON345_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR3071010.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSIQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7510..8496)
                     /gene="hybA"
                     /locus_tag="ON345_000907"
     CDS             complement(7510..8496)
                     /gene="hybA"
                     /locus_tag="ON345_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR3071011.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8499..9617)
                     /gene="hybO"
                     /locus_tag="ON345_000908"
     CDS             complement(8499..9617)
                     /gene="hybO"
                     /locus_tag="ON345_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR3071012.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9806..10093)
                     /locus_tag="ON345_000909"
     CDS             complement(9806..10093)
                     /locus_tag="ON345_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR3071013.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10212..11099)
                     /locus_tag="ON345_000910"
     CDS             complement(10212..11099)
                     /locus_tag="ON345_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR3071014.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11256..12296
                     /gene="gpr"
                     /locus_tag="ON345_000911"
     CDS             11256..12296
                     /gene="gpr"
                     /locus_tag="ON345_000911"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR3071015.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLMNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12336..12830)
                     /locus_tag="ON345_000912"
     CDS             complement(12336..12830)
                     /locus_tag="ON345_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR3071016.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13021..13905
                     /gene="yghA"
                     /locus_tag="ON345_000913"
     CDS             13021..13905
                     /gene="yghA"
                     /locus_tag="ON345_000913"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR3071017.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14177..14602)
                     /gene="exbD"
                     /locus_tag="ON345_000914"
     CDS             complement(14177..14602)
                     /gene="exbD"
                     /locus_tag="ON345_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR3071018.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14609..15343)
                     /gene="exbB"
                     /locus_tag="ON345_000915"
     CDS             complement(14609..15343)
                     /gene="exbB"
                     /locus_tag="ON345_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR3071019.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15595..16782
                     /gene="metC"
                     /locus_tag="ON345_000916"
     CDS             15595..16782
                     /gene="metC"
                     /locus_tag="ON345_000916"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR3071020.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16922..17581
                     /locus_tag="ON345_000917"
     CDS             16922..17581
                     /locus_tag="ON345_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR3071021.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17621..18520)
                     /gene="yqhC"
                     /locus_tag="ON345_000918"
     CDS             complement(17621..18520)
                     /gene="yqhC"
                     /locus_tag="ON345_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR3071022.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18714..19877
                     /gene="yqhD"
                     /locus_tag="ON345_000919"
     CDS             18714..19877
                     /gene="yqhD"
                     /locus_tag="ON345_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR3071023.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19982..20809
                     /gene="dkgA"
                     /locus_tag="ON345_000920"
                     /pseudo
     CDS             19982..20809
                     /gene="dkgA"
                     /locus_tag="ON345_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            21009..21935
                     /locus_tag="ON345_000921"
     CDS             21009..21935
                     /locus_tag="ON345_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR3071024.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21986..22243
                     /gene="yqhH"
                     /locus_tag="ON345_000922"
     CDS             21986..22243
                     /gene="yqhH"
                     /locus_tag="ON345_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR3071025.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22286..24505)
                     /locus_tag="ON345_000923"
     CDS             complement(22286..24505)
                     /locus_tag="ON345_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR3071026.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALLGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVMPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24616..26028)
                     /gene="ftsP"
                     /locus_tag="ON345_000924"
     CDS             complement(24616..26028)
                     /gene="ftsP"
                     /locus_tag="ON345_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR3071027.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26103..26840)
                     /gene="plsC"
                     /locus_tag="ON345_000925"
                     /gene_synonym="parF"
     CDS             complement(26103..26840)
                     /gene="plsC"
                     /locus_tag="ON345_000925"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR3071028.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27073..29331)
                     /gene="parC"
                     /locus_tag="ON345_000926"
     CDS             complement(27073..29331)
                     /gene="parC"
                     /locus_tag="ON345_000926"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR3071029.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVLRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29877..30357)
                     /locus_tag="ON345_000927"
                     /pseudo
     CDS             complement(29877..30357)
                     /locus_tag="ON345_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
     gene            complement(30410..30802)
                     /gene="ygiW"
                     /locus_tag="ON345_000928"
     CDS             complement(30410..30802)
                     /gene="ygiW"
                     /locus_tag="ON345_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR3071030.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30954..31613
                     /gene="qseB"
                     /locus_tag="ON345_000929"
     CDS             30954..31613
                     /gene="qseB"
                     /locus_tag="ON345_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR3071031.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31610..32959
                     /gene="qseC"
                     /locus_tag="ON345_000930"
     CDS             31610..32959
                     /gene="qseC"
                     /locus_tag="ON345_000930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR3071032.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     NTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
BASE COUNT         8059 a         8846 c         8366 g         7717 t
ORIGIN      
        1 gggttgctac aacatgtatt actttttcac cacaattaac ggttcaatat cactctcttt
       61 tttgatgacc agtgattcat caccgcgcaa acacgtccca ccgtagttgc cgcccacggt
      121 gaaggtacat acctgaatgt atttaccgtc cactttcggc aaacaccaca gttgctgata
      181 gatgtttttc tgctcggcaa atttaccgct ggttttgtcc agcacttctt catgatggct
      241 gaccaggtcg atattgctac cacagcgacc ggcaatcggt ttcactgcgt aacctgtttt
      301 caccaattca tcattaacag tgaaatcggt atccagcagg taacgatggt gcgggaacag
      361 cgaccagagg atcggcagaa tcgctttgtt gccggggatc accgtccaca gcggctcaaa
      421 gactagcact tccgggcgca gcaatacgtc gataagacgc acttcgtttt gcggatgacc
      481 ggtacggatt ggcaccgcag caaactcacg gtcgctaact tcacgaatct gatcaaacgc
      541 ggtttcccac gcccaggttt tccacacgca gttaaccagt cgcccttccc catcaatcag
      601 ttgcccggca gcatcccagc ccagttcatc caatccacgc aagatacgcg tttcaaagcc
      661 cgcctggtgc agcgcctgct ccataaactg cgcgtgatag ttttcctcga tatctttgtc
      721 ctgcatgata tggacaaacg gacgtgcacg actgtgtttc caggcaccag ccaattcgtt
      781 aatcagccct tccgccggat tgaagccgtt gcctttatag ccctgctccg cccaacgttc
      841 gaggatcaag cccgcttcgg tatgacagga ggcggagtcg gcgttgtact cgtaaacctt
      901 caggccacgc tcatccatgc agaaatccat acgaccagtg atcatatggt gacggcgacg
      961 ctgccaggag agacgcaaac gtggccagag gattttcggg atgtcgaata gcgccagcag
     1021 gttgtcatct ttcagcacct tgtcggttgc gtgaagatac atcaggtgca gctcgttggt
     1081 ggctttaatt agctcctgct cggcactctc ggtaatggtg tagtaatgat aaggatcctg
     1141 attgatcacc tgaccgttgg cctgcacata ggcgttttgc agcggatctt tttcatccag
     1201 ccatttaccg tcaaactggc ctttgttttc caggcgcgct ccgctgattt tcagcagctc
     1261 gcctgcaatt tccggctgcg gtaagctgta ttcagtatct tccgtctgga tcatccagcc
     1321 cagaatggtg gtgtcatcaa aagtgtcttt cagggtatag cagccgtttt cgaccaccat
     1381 ctccagctca cgcgtccact gttgcccttg cggcaacggg gaatgaatca cgttctgttc
     1441 cgcaatacgg actttgttgc catgcaattg ggtaatgatg gcgacatggc cagtgtcttt
     1501 aaattcaccg cctttatccc agataagaag cgcacccgcg accggcgcac gcggcgagcc
     1561 gttaggaaat gcctgcaatg gcaggatgtt gtcattaacc acttctcgca ggaagcgcag
     1621 cgagaaaatc tcccacgcca tacccacgtc agtaaagacc acaccgtaat tcagaaagag
     1681 aaaacggcga gcaaattcaa cgcattgcca cttgtggccc atatattcgt cgtcgatata
     1741 gctacggaat acggcgtcat cttcgtattc ctgcggatcg agagaactgt aatctgaaga
     1801 gtagattgct accccacctg gggcgtagcc caataatgtc ccgaacgggg catcctggct
     1861 ggtcgttcct ttgctcatca ctttaccttt aacaatacaa cctaagcagt tggcgtaatt
     1921 gttgtgctct gattacctgc tcagcgacat taaacgtaca gaggtcagac taatcataca
     1981 cctcatcgca gcggctggcg caaagggtta aaaaattcac attctgtaca gcaagtgacc
     2041 tgctacactg cttcaacact accactcaga aggcaactca ctatgacaga caatacttat
     2101 cagcccgcga aagtctggac gtgggataaa tccgctggcg gcgcgttcgc caatatcaat
     2161 cgcccggttt ctggtccgac gcatgaaaaa acgctgcccg ttggcaaaca cccattgcaa
     2221 ctttattcgc tgggaacgcc gaacggtcag aaagtaacga ttatgcttga ggagctgctg
     2281 gcgctgggcg ttactggtgc agagtacgac gcctggctga ttcgtattgg cgatggcgat
     2341 caattctcca gcggctttgt cgaagtgaac ccaaactcga agatcccggc gctgcgcgat
     2401 catacgcata atccgccgat ccgcgtgttt gaatctggtt ccatcctgct ttatctggcg
     2461 gagaaatttg gctacttcct gccgcaggat ctggcgaaac gtactgaagc aatgaactgg
     2521 ctgttctggc tacagggggc tgcgccgttc ctcggcggtg gttttggtca cttttaccat
     2581 tacgcgccgg tgaaaattga gtacgccatc aaccgcttta ccatggaagc caaacgtctg
     2641 ctcgacgtgc tggataagca actggcgcag cataagtttg ttgcgggcga tgagtacacc
     2701 attgcggata tggcgatttg gccgtggttt ggcaatgtgg tgttaggcgg cgtgtatgat
     2761 gccgcagagt ttcttgatgc aggtagttat aaacacgtac aacgctgggc gaaagaagtg
     2821 ggcgaacgtc cggcggtgaa gcgtgggcgt attgttaacc gcaccaacgg gccgctgaac
     2881 gagcagttgc atgagcgcca tgacgccagt gattttgaga caaatacaga agataagcgt
     2941 caggggtaag ggttggtgtt cgtcgcagca agccatccag gccggataag gcgttcacgc
     3001 cgcatccggc aatcgtgcac aatgcctgat gcgacgctgt cgcgtcttat caggcctacg
     3061 aaagctaatc atcacgcgct ggtaatgtcg taatccattt ggcgcagtgc gtctaatgtg
     3121 gctttggctt catcttcgtc gatgatgctc atggcaaatc cgacgtgtac cagcacccac
     3181 tggcccagca gatcggcagg gttaccttca caaatcaggg cgatattcac gtcgcgcttg
     3241 ataccacata cttcaacctg cgcaagctgg tgaatatctt caccgacagc cagcacctgg
     3301 cctggaacgc caatacacat tattaactcc ggttattcaa cttcaatact tttgacgatc
     3361 agcgaatcgc cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg
     3421 tgctgatgaa tctccaccac ctggctgcaa tcccagcacc aagcctgggc gggtttatag
     3481 acgatatgta aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa
     3541 cggacggcgc tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg
     3601 cgcttaacat cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaaga
     3661 gacaactcat gcattttcgc cactcctgcg accaaacagc agggcgcgac gccctgcgtg
     3721 tggtacatcc ggattcgtga ctggcagcga aaggatcatt cgtgcgcagt catccgtcag
     3781 gcgttggccc tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag
     3841 atattgcgaa acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg
     3901 cagaccaatt ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat
     3961 aactgcactc agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt
     4021 aaaatcagaa acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg
     4081 ccgggcaatt tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc
     4141 tatctcttca gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg
     4201 cagccagaac ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga
     4261 tgtgtggctc cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac
     4321 cggtgaagcg cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt
     4381 tggtaaacaa cgccggaact tcttcatccc gcaggatcat catcgttccc ggcgtgttct
     4441 ttttcgacac aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca
     4501 gctccattcc cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt
     4561 gctctaacgc ttcgacaatc cgcacaccga tggcttcatc ggtcagcaaa atattgccga
     4621 cccctaagac taagatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc
     4681 atccactacg tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac
     4741 ttccagcggt ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc
     4801 gacgtcatca ttgaagttac gcggaccaga gttccaggtt gatggaacaa ctgcctggta
     4861 gttactgata ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc
     4921 gaggaagcca acacctttga actcacccgt tgccggaatg ttcggtttca caaaggtggt
     4981 gtgatcgcct ttgccgatat tggtgatcag tgcactgtat tgattttgca ggatatcctg
     5041 caattcgcag cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgcgc
     5101 cacttccagc gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt
     5161 ctgggtagat tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc
     5221 taccgttttg ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca
     5281 accatcataa gccggaatgg tggtaccttc ccacggtgcc tgcggcgctt cgtctttata
     5341 ccaggagtgc ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt
     5401 gatcggacga tacgaggaca gatccgcatt ctcaatgtag ccgcccggga acaggaagct
     5461 gccgttttta ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc
     5521 gcgcgtcagc cattccgggt agaacgcggc aataactgcg gtatcaacct tataaacctg
     5581 ttcaacaaag tcgctcagct tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt
     5641 cagcacgccc aaaccgtcga ggttgatcgg gttcgcgaca ccacctaccg ccaggttctg
     5701 aatgtgcggc gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc
     5761 cagcgcctgc aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc
     5821 cgggtgaccc cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt
     5881 gatcttgttc tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc
     5941 tttcagcatt tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca
     6001 gtccagcgcc gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag
     6061 aatgatgtta cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc
     6121 cgcacgaacg gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat
     6181 catccatgca tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt
     6241 accggaagcc catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg
     6301 cccctcaata cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca
     6361 tgacgattat gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg
     6421 atgtaagcgc aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc
     6481 gggaagtaag cgtaaccgcc gcccgggttg aatgccacca gcgaataggt cagacgccag
     6541 gttgcacaac ctaacagtgc gctcagtgct gacaggaaca gcatgcggga atcattacgc
     6601 agcttcgcca cacgcagaac caccagcggg aagagcatca gcaggacttc aatccagaac
     6661 atcacggagt agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg
     6721 ccaaagcgca gcacgatgaa aatcgccagc aacacactga tggtgttggt cagcttaaca
     6781 aacagactct tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca
     6841 aagatgacaa tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt
     6901 tcatagctct gccacagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt
     6961 gaagactggt gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg
     7021 ttcagacgct gtatcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc
     7081 agagccatca cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc
     7141 acgttgaagt gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata
     7201 gtgatcgaca agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc
     7261 agcggatggt attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca
     7321 caggcaaagc cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg
     7381 ccgttcaagt cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata
     7441 cagatgacga ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc
     7501 ggttgtggat catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg
     7561 acgaaccagc acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag
     7621 ggtgtgttga atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc
     7681 gagattttca taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc
     7741 gccgtacaga tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag
     7801 cgtctgacgc ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc
     7861 cgccatcagc tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca
     7921 gccaggcaga ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt
     7981 atgcagcgca ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac
     8041 catgcagtaa cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg
     8101 atcttttttc agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat
     8161 acactgtttc ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc
     8221 gctggtccac acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca
     8281 ggtctgttcc ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca
     8341 agcctggcag cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg
     8401 cgggcggttt tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc
     8461 gcaggaggct gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg
     8521 ttatctttct tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc
     8581 agcccaacca caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta
     8641 gcgttaacat ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg
     8701 atgcctttat ggaagccgat accttcttcg ttacagccat agcatgggtg accaatcgct
     8761 accggccaca cgccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct
     8821 ggccctttac agccgaggtg gtacaagcac cagccttcac ggtggccttc atcaccgaac
     8881 tctttggcaa aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga
     8941 cggccatagg cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg
     9001 atgatgtgcg caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg
     9061 ataacggttt tgcctggcag aacttcttgc aggctgactg cgccagttgg gttaactcca
     9121 gctgcggcaa caccgcccca cgcagagcag gaaccgatag cgataatcgc tgctgcgcct
     9181 tctgccgctt tgcggatgtg atccacaatc ggctcaccag caaccatgca ataaataccg
     9241 ttatctttta atgggatgga accatccaca accaacacat actgcccttt gtacttctca
     9301 agtgcgttat gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga
     9361 tactccagag agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc
     9421 agagattccg tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc
     9481 gggttagtaa ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg
     9541 gctaatgctg cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg
     9601 gtgttatctc cagtcattta tagttattcc gttgcgaaga cctggcatat attttgcctc
     9661 aatcgcaaaa tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg
     9721 atatcatgat actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt
     9781 aaagcaagat aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc
     9841 gcaacgtaga acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc
     9901 catagcggcg cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt
     9961 cgtacatccg gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg
    10021 tcacattaac cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa
    10081 aatggttatt catcgttcac ctcattcgct atatatcgcc atatatagcg atattttagg
    10141 caaagaacga cttttgcgca cgctattttt tcatgcttgt ttaagacgta atccagtaag
    10201 agataaacct atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga
    10261 tcggcggcag atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc
    10321 ggatagatat acgcctcata aaccttatta ttggctttca acgccgcctc gtaagcaggc
    10381 cagccctcgt tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc
    10441 ttcgccacat cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc
    10501 ggatacgcga cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc
    10561 actttgcctg ccgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc
    10621 atcagtttgg ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga
    10681 taacctccaa cggaacttaa gccgtcaggt gccagggcga tataccccgc cttcgccact
    10741 cgccgtgcca catcttcgat atacggattc agtccacgat tctcatgcac caccaccacg
    10801 gctggcgttt tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga
    10861 ccatttggcg aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc
    10921 tgagtcgcca gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg
    10981 cctaccgcat acttcgccgc aaggttgagg aactcacgtt tcgagatttt cccgtgagcg
    11041 taatagtcgt agtaatccag caactcttgt gggaaatctt tggcggttaa acgcggcatc
    11101 gttgcactcc tcagttggtg tttttttaag caaagcataa gcacgtgttt ttgcccagtt
    11161 tttcgtcact ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac
    11221 attgtttcga aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac
    11281 gttacgggca gatgcaatac cgctattgcg gaaaaagcgg tttacgcctg cccgcgttat
    11341 cgctcggttt atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcgatcc
    11401 tgcgtaaagc gtttgatttg ggcattacgc actttgattt agccaacaat tacgggccgc
    11461 ctccaggaag cgcagaagag aactttggtc gcctgctgcg ggaggatttt gccgcttatc
    11521 gcgatgaact gattatctct accaaggctg gctacgatat gtggcccggc ccttacggct
    11581 ctggcggttc acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc
    11641 ttgagtatgt cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa
    11701 ccgcctctgc gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct
    11761 cttactcgcc agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc
    11821 ttttaattca tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc
    11881 tggataccct gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat
    11941 tgctgaccgg aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga
    12001 ataaagttcg tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct
    12061 taatgaatga aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt
    12121 tgctgaaaga tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag
    12181 aggagaacgt gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg
    12241 atcagcatat cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg
    12301 ttaataacgg gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg
    12361 tcaagatagc ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac
    12421 atcagtttgt tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa
    12481 gaaattgcca caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc
    12541 agccagttca gcttctcttt gttctcggag atatcgagtt gtgagacgaa attctcataa
    12601 ccggaaaaca tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc
    12661 aacacgagga tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc
    12721 tcctggaaga acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg
    12781 gcaagcagcc agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt
    12841 cgtgtagtta aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat
    12901 caacatatac gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc
    12961 ttatcaacgc atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg
    13021 atgtctcatc taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa
    13081 caaccgacgc caggcatcca ggcgaagatg acaccggtac cggattgcgg cgagaaaacc
    13141 tatgttggta gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga
    13201 ataggtcgcg ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat
    13261 cttcccgtgg aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggtcgc
    13321 aaagccgttc tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac
    13381 gaagcgcaca aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt
    13441 gccattcctg atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac
    13501 gttttcgcgc tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt
    13561 atcatcacca cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg
    13621 gctacgaagg cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa
    13681 ggtattcggg tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttctggc
    13741 ggacaaacgc aggataagat cccgcagttt ggtcagcaaa cgccgatgaa acgtgcgggg
    13801 caaccggcgg aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc
    13861 accgcagaag tgcacggtgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg
    13921 tgaaaagcaa ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta
    13981 tccgcctaca aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc
    14041 gtagcgcatc aggcattgag cacctaatgc cggatgcggc gtgaacgcct tatccggcct
    14101 acaaacgcac gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc
    14161 atcaggcaat tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca
    14221 ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt
    14281 tatccgctcg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg
    14341 taatcattgt ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca
    14401 ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt
    14461 tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca
    14521 acatcacgtc gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt
    14581 tttcgttaag atgcattgcc atcggatatt atcctgcgcg taatttttgt gcgacacgaa
    14641 ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc aacaatacct
    14701 gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat
    14761 agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg
    14821 cgatacccgg cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc
    14881 tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg
    14941 ttgccagata gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca
    15001 gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca
    15061 gctcattctg cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg
    15121 cgatatcgtt ggcctggttt aaggaacgcg cttcagccag cagttgctgc tcgcgcttaa
    15181 ggcgacgctt ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta
    15241 cggaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg tgctgataca
    15301 taccccagac ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg
    15361 caagtcacaa aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca
    15421 ttactatttg catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa
    15481 gtcaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc
    15541 gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc cgagatggcg
    15601 gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata cactctcggc
    15661 gcggtaaata gcgtgattca gcgcgcttct tcgctggtct ttgacagtgt ggaagccaaa
    15721 aagcacgcga cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta
    15781 acccatttct ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgtgcta
    15841 tttccctgcg gggcggcagc ggttgctaat tccattcttg cttttgtcga acagggcgat
    15901 catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc
    15961 agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag
    16021 catctgcagc caaacactaa aatcgtgttt ctggaatcgc caggctccat caccatggaa
    16081 gtccacgacg ttccggcgat tgttgccgcc gtgcgcagtg tggtcccgga tgccatcatt
    16141 atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat
    16201 gtttccattc aggccgctac caaatatctg gttgggcatt cagatgcgat gattggcact
    16261 gccgtgtgca atgcccgttg ctgggagcag ctgcgggaga atgcctatct gatgggccag
    16321 atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt
    16381 ttgcgtcaac atcatgaaag cagtctgaaa gtggctgaat ggctggcaga acatccgcaa
    16441 gttgcgcgag ttaaccaccc tgctctgcct ggcagtaaag gccacgaatt ctggaaacga
    16501 gactttacag gcagcagcgg gctattttcc tttgtgctta agaaaaaact cagtaatgaa
    16561 gagctggcga actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg
    16621 tatgaatcgt tgatcctggc gaatcaacca gaacatatcg ccgccattcg cccccaaggc
    16681 gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat
    16741 ctgattgccg atctggacgc cggttttgcg cgaattgtat aacattgcca cttttggaca
    16801 attttgcaga catttaattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc
    16861 cggacgaatc aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc
    16921 catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc
    16981 ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa
    17041 cggcctgctg cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt
    17101 gattgcccaa ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag
    17161 tctgggctgc tggctaagtt atattcaggg gcgctggtta gggaatacca aaacggtgaa
    17221 aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg
    17281 ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc
    17341 aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag
    17401 tggattgctg tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt
    17461 cgttaaacgc catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt
    17521 aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg
    17581 acgattttcc ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc
    17641 gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa
    17701 attgcgatgc gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat
    17761 ggatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg
    17821 tgctggtgac agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg
    17881 ccagttgctc gacgctcagg ttttcggtgt atttattctc aatccgtttc agcacgcggc
    17941 taatcagact gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc
    18001 cggtcagcac gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca
    18061 gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg
    18121 tggcggagtt aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt
    18181 ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca
    18241 cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt
    18301 cattggcatc ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga
    18361 gaattatgat gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca
    18421 acaaacgcac atcgggcaac agttctgaca aactattttc tttaattttc agtttattaa
    18481 ctttatccgc cagcaagcgg caaatctctt cacgtttcat atcgcgtaat ttcttaggaa
    18541 taatgcggca atttgattgt gcgcaatttt gtagcatttc tccagcactc tggaggaata
    18601 ggcaagacat tggcagaaat gagcattgag agccaaggcg ctggcgatca caatgaaaaa
    18661 catcaggcag atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca
    18721 actttaatct gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac
    18781 gcgaacaaat tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa
    18841 aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg
    18901 gcggtattga gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg
    18961 aacagaaagt gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat
    19021 ttatcgccgc agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg
    19081 gcggtaaaga gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg
    19141 gttcagaatc caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt
    19201 tccattctgc ccatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc
    19261 tgccgccgcg tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt
    19321 atgttaccaa accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga
    19381 cgctaatcga agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca
    19441 acgtcatgtg ggcggcgact caggcgctga acggtttgat tggcgctggc gtaccgcagg
    19501 actgggcaac gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc
    19561 aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta
    19621 agctgctgca atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta
    19681 ttgacgccgc gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc
    19741 tctccgacta cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc
    19801 acggcatgac ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat
    19861 acgaagccgc ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag
    19921 acttaagttc acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg
    19981 tatggctaat ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact
    20041 gggcgtctgg caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt
    20101 gggttatcgc tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc
    20161 cctgaaaaat gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga
    20221 cgaccacaag cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta
    20281 tatcgacctc tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg
    20341 gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt
    20401 ccagatccat cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca
    20461 gatcgaactt catccgctga tgcaacaacg ccagctacac gcctagaacg cgacacacaa
    20521 aatccagacc gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa
    20581 agtcattcgc gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg
    20641 gcatctggat agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga
    20701 aaactttgat gtctgggatt tccgtctcga caaagacgaa ctcggcgaaa ttgcaaaact
    20761 cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac atgcaaattc
    20821 tcccggtggc ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt
    20881 cccgcccgtt gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag
    20941 agccggaagc tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg
    21001 aaaaacagat gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg
    21061 cacagccccc ctcactgacc gtagaacaaa cagtccggca tatttatcag aactataaat
    21121 cagatgccac tgccccttat tttggtgaaa ccggagagcg ggcgataact tctgcgcgta
    21181 ttcaacaggc gcttaccctg aacgacaatc ttacgctgcc gggcaatatt ggctggctgg
    21241 attatgatcc ggtttgtgat tgtcaggatt ttggcgatct ggtgctagaa agcgttgcga
    21301 taacccaaac tgacgccgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg
    21361 ataaagaaaa gaccacgcag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg
    21421 acgatattgt cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa
    21481 cgctggccgc tttagcttcg ttgcaaaaag aacagccgga agcctttgtt gccgaactct
    21541 ttgaacatat tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc
    21601 aggcggttaa tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata
    21661 tgcaaataga acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat
    21721 tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg
    21781 ttcgttttac gctgaccaat ggcaacaacg aagagcaaga actggtttta cagcggcgcg
    21841 aaggcaagtg ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga
    21901 ttgaggcaaa aactgccgcc agattaaagc aatgagctga attaaataac aattagccgg
    21961 aacaataaat aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct
    22021 ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcagc tggcgagcaa
    22081 tgtgcaaaca ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc
    22141 acaaatctat gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga
    22201 ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa tgataaaata atccccggca
    22261 gcatgtcagt tgccggggat attttttaac gtccaaccgc cgctttaggc cgtttcttcg
    22321 caccagcatt caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct
    22381 gacgctgggt cgccatcggc gtatgtttcg tcaacgccgg acgggtattg cggttctggc
    22441 gacgagcttc acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc
    22501 caatcagatg ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg
    22561 gatcgtggta acgcaacaac gctttatgca aacgacgctg tttgtcaccc ttcggtacaa
    22621 agacgtcttc actcttataa ccaatcttcg ccagcgggtt tttcccggtg taatacatgg
    22681 tggtcgagtt agccagcggc gatggataga agttctgtac ctggtcgaga cggaaacgat
    22741 gctttttcag ccacagcgcc agattcacca tatcttcatc acgcgtaccg gggtgcgcgg
    22801 agatgaaata ggggatcaga tactgctctt tacctgcctg ttttgagtaa gtatcgaaca
    22861 gctctttaaa gcggtcatag ctgcccatgc ccggcttcat catcttcgat aacggccctt
    22921 cttcggtatg ttccggggca atcttcagat aaccgccgac gtgatgggtc gccagctctt
    22981 tgatatagcg tggatcttcc acggcgatgt cataacgtac gcctgaggca atcaggatct
    23041 ttttaatgcc tttcagatcg cgagcgcggc gatagaggtt gatcgtcggt tcgtggttag
    23101 tgtccatatg cggacaaata tccgggtaaa cgcaggacaa acggcgacaa gtttgctctg
    23161 cgcgtggcga tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa
    23221 tcacgcccgt aaaacctgga acggtgtcgc ggatcgcttc aatctcatta atgatcgaat
    23281 cttccgaacg gctctgaata atgcgccctt cgtgctcggt gatagaacag aaagagcagc
    23341 cgccaaagca gccgcgcata atgttgaccg agaaacggat catttcgtaa gccggaatac
    23401 gggcattgcc ataggccgga tgcggcacgc gcttgtacgg cagcgcaaag acgctgtcca
    23461 tctcttcggt agaaagcgga atagcaggcg ggttgatcca cacatagcgg tcgccatgtt
    23521 tttgcatcaa tgcgcgggcg cagcctgggt tagtttcgtg gtgcagaata cgcgaagcat
    23581 gggcgtacag cactttatcg cccttcactt tctcgaaaga aggcagcaac acgtaggttt
    23641 tttcccacgg tttcgggcgc ggcggctgca cggttacggc tttggcttcc tgctttttcg
    23701 gcatcaccgg tttgttatcc gcacacggca aatcttcgcc atacggatgc ggtatcgggt
    23761 cgattttccc aggagtatca agacgagtag aatccacgcc gctccagcct agcaacgcct
    23821 cttttacgat aatcgcagta ttacgcacat cgcggatttc gctaattggc tcgcccatcg
    23881 ccagacgatg cgccacttcc accagcggac gctcaccgtt accaaacatc agcatgtcgg
    23941 ctttcgaatc caccagcacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg
    24001 tacggcgcag actagcctca ataccgccga ggatcaccgg tacatctttc cacgcctctt
    24061 tacaacgctg ggtataaacc agtgtggcgc gatccgggcg cttacccgcg acgttatccg
    24121 gcgtgtaggc atcgtcatga cgtaaacggc gatcggcggt ataacggttg atcatcgaat
    24181 ccatgttgcc agcagtaaca ccgaaaaaca gattcggttt acccagacgc ataaagtcgt
    24241 ctttgctgct ccagtccggc tgggcgatga tcccgacacg aaagccctgc gcttccagca
    24301 tacgaccgca aatcgccatc ccgaagcttg ggtgatcaac atacgcgtcg ccagtaacca
    24361 aaatgatgtc gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa
    24421 acggtgccgg tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg
    24481 gttggatcag ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg
    24541 ccggggatta taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc
    24601 gggcgagtat gaagattacg gtaccggatt gaccaaaagt tgccctatcg agccacggtc
    24661 cgccatttcc agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc
    24721 gaaataaacc agcagctcca cttgtccgtc aacccaaaca gtatctttcc agcctctgtc
    24781 ttccgggaac ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg
    24841 gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt
    24901 gacatcaata cggttgacgt cccacagctg cccattaata cccgggtcat cacccagact
    24961 gatatcgcga ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg
    25021 aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ctagggtaga
    25081 aaccagaata ctggatggct caaagaagcc acgaatacga tcaacaatgc tcgccgcttc
    25141 gccacaggtg atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc
    25201 gcccggtgcc agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc
    25261 agaaatcaca tgtaacgggc gaccatcgtt catctgtagt tgatagcgac gagagttcga
    25321 cgcgttcagc agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac
    25381 accgttaacc agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg
    25441 cgtaccaaag ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata
    25501 atggttgggg ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag
    25561 gccgttatag acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc
    25621 tgcgttctgg cgaatgggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc
    25681 cggaccgccc atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc
    25741 tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg
    25801 ccccaggtaa cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca
    25861 gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg
    25921 aacgggtagc ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca
    25981 aagtgcaatc cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg
    26041 taaaactggc taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg
    26101 acttaaactt ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt
    26161 tgttccatta tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga
    26221 ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga
    26281 ttaatcttat tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt
    26341 gccgcgtgaa atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct
    26401 tccgggaaca tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg
    26461 gtgccgtgag ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac
    26521 aactgcccga agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt
    26581 tgcacaatgt tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata
    26641 gcattgccgt agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagc
    26701 ggcgcaagac ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg
    26761 aaaaggcagt aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata
    26821 agacgaaaga tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca
    26881 cctgaaatca ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg
    26941 caacgcatga ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg
    27001 ctgccggatg acgacttaac gtttcatccg gcgttccttg caagcgggag gaaacaggcc
    27061 ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc
    27121 aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagt
    27181 gactttctgt aactcttccg ggcgcagttt aattttgcgt ttcccaacat gaatggtcag
    27241 cgtgctttgc ggcggcagaa cgtacaattg cgccagacca tcttctccac gcgcggcttc
    27301 tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct
    27361 taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc
    27421 ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca aagccttacc
    27481 tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga
    27541 agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgccccagg
    27601 cggcaacgtt aatttgccgg tgagtggttc gccctgacca cgcgccgacg gcagcgtaat
    27661 cgggtcgatg gcatagctac gaccggtgga atcaacaaac actaccggtt ggttgctctt
    27721 acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg gcgcgtcgat
    27781 atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg tgacaggttc
    27841 agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga
    27901 acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt
    27961 cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt ccagctcact
    28021 ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag
    28081 gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc
    28141 atcttcgtta cgaatgatct caatcacttc gtcgatgttg agaaacgcca ccagcaaacc
    28201 ttccaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac
    28261 ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg
    28321 acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt
    28381 agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgca gcacaatcac
    28441 cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt
    28501 tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg
    28561 taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga
    28621 accacgtccg ttctcgtaga ttttacggat ctcggcgcgc gaagtgataa tttccgcttc
    28681 agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtgg ttttcggctg
    28741 gtcgattaat gcgattgccg cctgagctac ttcacgcagg ttatgcggtg gaatatcggt
    28801 cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgagcagg
    28861 cagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg
    28921 ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg tgtaacgcat
    28981 tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg
    29041 ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg cgctatcgcc
    29101 gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc
    29161 gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa caggtttaag
    29221 accatcgcca ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta
    29281 ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga
    29341 ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct
    29401 gccgcgtcga gtcacaggag atcagcgtga atagcgccac cggacgtgct ccgacggcga
    29461 taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc ggcatagagg
    29521 gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc cgggcgattc
    29581 gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt cgactgtaag
    29641 tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc ctgccataag
    29701 tgcggcttgc tcatggatac ccccgagacg gcttacggcg caccggataa taaatttcgc
    29761 gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat
    29821 tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac
    29881 gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc ggcaagattg
    29941 gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg
    30001 cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg
    30061 gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt
    30121 tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcagggtcg
    30181 tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc
    30241 cacatgataa atttcgccac tgtagttgag caactcaggg ctacagcggt gcggcaacat
    30301 cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt ttgtcatttt
    30361 gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat tacggattta
    30421 ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg acttcaccct
    30481 gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt ccagcgtttg tggtcgatat
    30541 caacattgat agtaccgctg gcatctttaa acacgtagag atcgtcagag atgcgttcaa
    30601 cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg ctttctacgg
    30661 tcgttacgct gccgttcggc ccctggaatc ctccggcctg actttgcgtt gccgatgggc
    30721 cagaaaaacc gccctgctct gctgccatca ccggtgcgct gcacagggcc attactgcga
    30781 ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc cgagcattta
    30841 acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttagtgc tgtacagagc
    30901 gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa aaaatgcgaa
    30961 ttttactgat agaagatgac atgctgattg gcgacggcat caaaacgggc cttagtaaaa
    31021 tgggttttag cgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg ctttatagcg
    31081 cgccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt cgcgatattt
    31141 tgcgcgaatg gcgagaaaaa ggtcagcgtg agccggtact gatcctgacc gcgcgcgatg
    31201 cgctggagga acgtgtagaa gggctgcgtc tgggagctga cgattatctg tgtaaacctt
    31261 ttgcgttgat agaagtcgcc gccaggctgg aagctctgat gcgccgaacc aacggccagg
    31321 ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt atcgccacgc
    31381 tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc
    31441 gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat acctgggacg
    31501 aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc aaactcggta
    31561 gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa tgaaatttac
    31621 ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt ttaattctgg cctcggtgac
    31681 ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg atgaattgtt
    31741 caacacccaa ctgatgctgt ttgccaagcg gttaagtacg ctcgatctca acgaaatcaa
    31801 cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg ttgatgacga
    31861 tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaacg atggcgataa
    31921 cggagaagat attccctata gctatcaacg ggaaggtttt gctgacgggc aactggtggg
    31981 cgataaagat caatggcgtt ttgtctggat gacctcacct gatggcaaat atcgcatcgt
    32041 tgttggtcag gaatgggaat accgtgaaga catggcgctg gcgattgttg ccgggcaatt
    32101 gatcccgtgg ctggtcgcac tgccgattat gttaatcatc atgatggtac tattgggtcg
    32161 tgaactcgcg ccgctgaaca aactggcgct ggcgctgcgt atgcgtgacc ctgactcgga
    32221 aaaaccacta aacgcgactg gcgtacccag cgaagtgcgt ccgttggttg agtcgctaaa
    32281 tcaactgttc gcccgcacac atgcgatgat ggttcgtgaa cgacgcttta cctccgacgc
    32341 agctcacgaa ctacgtagcc cgttaacggc gctgaaagtg caaaccgaag ttgcgcagct
    32401 ttctgacgat gatccgcaag cgcggaaaaa agcgctgctt caattacatt acgggatcga
    32461 tcgcgctact cgtctggttg atcaactgct cactctatcg cggctggact cactggataa
    32521 ccttcaggac gtcgcggaga tcccgcttga agatctcctg caatcgtcgg tgatggatat
    32581 ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg cccacggcat
    32641 caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc tggataacgc
    32701 cgtgcgctac agtccacagg gcagcgtggt agacgtcacg ctgaatgctg ataatttcat
    32761 cgtgagggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt
    32821 ctatcgccca cccggacaaa ccgctaccgg cagcgggctt gggctatcga ttgtccagcg
    32881 aatcgccaaa ttgcatggca tgaatgttga atttgggaat gcggaacaag gtggatttga
    32941 ggcgaaggta agctggtaac ttctctgtct atgagagctg ttaaaacg
//
LOCUS       DAJSKJ010000022        32576 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000022 DAJSKJ010000000
VERSION     DAJSKJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.022"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(16..297)
                     /locus_tag="ON345_000931"
     CDS             complement(16..297)
                     /locus_tag="ON345_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR3071033.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(560..1669)
                     /gene="apbC"
                     /locus_tag="ON345_000932"
     CDS             complement(560..1669)
                     /gene="apbC"
                     /locus_tag="ON345_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR3071034.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1801..3834
                     /gene="metG"
                     /locus_tag="ON345_000933"
     CDS             1801..3834
                     /gene="metG"
                     /locus_tag="ON345_000933"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR3071035.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3975..7769
                     /locus_tag="ON345_000934"
     CDS             3975..7769
                     /locus_tag="ON345_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCR3071036.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFTASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEVLYPGILQVKDACTADSLAEFAWDLFNAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7779..8585
                     /locus_tag="ON345_000935"
                     /pseudo
     CDS             7779..8585
                     /locus_tag="ON345_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8575..9063
                     /locus_tag="ON345_000936"
                     /pseudo
     CDS             8575..9063
                     /locus_tag="ON345_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9074..11353
                     /locus_tag="ON345_000937"
     CDS             9074..11353
                     /locus_tag="ON345_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071037.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAVLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQIPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11346..12482
                     /locus_tag="ON345_000938"
     CDS             11346..12482
                     /locus_tag="ON345_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3071038.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHHVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12479..14479
                     /locus_tag="ON345_000939"
                     /pseudo
     CDS             12479..14479
                     /locus_tag="ON345_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            14604..15065
                     /locus_tag="ON345_000940"
     CDS             14604..15065
                     /locus_tag="ON345_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR3071039.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIACVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(15105..15575)
                     /locus_tag="ON345_000941"
     CDS             complement(15105..15575)
                     /locus_tag="ON345_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR3071040.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(15622..16341)
                     /gene="yehT"
                     /locus_tag="ON345_000942"
     CDS             complement(15622..16341)
                     /gene="yehT"
                     /locus_tag="ON345_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR3071041.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(16338..18023)
                     /gene="btsS"
                     /locus_tag="ON345_000943"
     CDS             complement(16338..18023)
                     /gene="btsS"
                     /locus_tag="ON345_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR3071042.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            18245..18976
                     /gene="mlrA"
                     /locus_tag="ON345_000944"
     CDS             18245..18976
                     /gene="mlrA"
                     /locus_tag="ON345_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR3071043.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            19036..19143
                     /locus_tag="ON345_000945"
     CDS             19036..19143
                     /locus_tag="ON345_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR3071044.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(19124..19855)
                     /gene="yehW"
                     /locus_tag="ON345_000946"
     CDS             complement(19124..19855)
                     /gene="yehW"
                     /locus_tag="ON345_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR3071045.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(19860..20786)
                     /gene="yehX"
                     /locus_tag="ON345_000947"
     CDS             complement(19860..20786)
                     /gene="yehX"
                     /locus_tag="ON345_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR3071046.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            complement(20779..21936)
                     /gene="yehY"
                     /locus_tag="ON345_000948"
     CDS             complement(20779..21936)
                     /gene="yehY"
                     /locus_tag="ON345_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR3071047.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSIFALILVQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(21943..22860)
                     /gene="osmF"
                     /locus_tag="ON345_000949"
     CDS             complement(21943..22860)
                     /gene="osmF"
                     /locus_tag="ON345_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR3071048.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(23112..25409)
                     /gene="bglX"
                     /locus_tag="ON345_000950"
     CDS             complement(23112..25409)
                     /gene="bglX"
                     /locus_tag="ON345_000950"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR3071049.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            25605..27320
                     /gene="dld"
                     /locus_tag="ON345_000951"
     CDS             25605..27320
                     /gene="dld"
                     /locus_tag="ON345_000951"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR3071050.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFNQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(27359..28291)
                     /gene="pbpG"
                     /locus_tag="ON345_000952"
     CDS             complement(27359..28291)
                     /gene="pbpG"
                     /locus_tag="ON345_000952"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR3071051.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(28465..29052)
                     /locus_tag="ON345_000953"
     CDS             complement(28465..29052)
                     /locus_tag="ON345_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR3071052.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            29222..29800
                     /locus_tag="ON345_000954"
     CDS             29222..29800
                     /locus_tag="ON345_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR3071053.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(29930..30691)
                     /locus_tag="ON345_000955"
     CDS             complement(29930..30691)
                     /locus_tag="ON345_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3071054.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGLRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(30744..32180)
                     /gene="mdtQ"
                     /locus_tag="ON345_000956"
     CDS             complement(30744..32180)
                     /gene="mdtQ"
                     /locus_tag="ON345_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR3071055.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQRLDASIQLTGALGGGYKR"
BASE COUNT         7994 a         8740 c         8238 g         7604 t
ORIGIN      
        1 aatcaggttg ccaaattaat cgtagcgatt taacacgatt cgacgtttgc tatcgctacc
       61 tgcaacaacc acttctgtcg ttacaccttt gacgggcgaa gaaaacagat gacgggtgtc
      121 aacattatac gttaactgac cacaatgctg ttgaggttga ccattaacca tagccatact
      181 gcatgttggg ggactgatcc tgccattaat cgctaatgtg gcagtctctg aagagaatgc
      241 gggagaagcc aatccatagg caagaaaaat aattcctgac agccaatact tattcattga
      301 acgttatccc tgtagtaaag gttatgcctg gatagaatga gtgcataaca aactatagct
      361 gtacatccac tacacagcca cgaaggatga taatgaagca ttgcctgtat gatcaatcga
      421 ctttgtagag tttcggacga aggtccgcag aatattcgca gtattaaata agtgttcatt
      481 aatgcggcaa aaactaatac accgcatcaa tgtaacatct ctgcggtgta tgaagaataa
      541 ccagaatggt taataggcgt tagaccgcgc ggaaggaaat ctcgcctgga atgacttcac
      601 cctgccagta gagctgagct gcaacgcggt cagccagctg gcggtagata gcggtaaatt
      661 cgctctccgg acggctgata accgttggcg tgcctttatc gagatcctca cgtaaggaga
      721 tgtgtaacgg catctgcccc agcagctgtg tgtggtattt ttcagccagt ttctctgcac
      781 caccggtacc gaaaatcggt tcatggtgac cacagttact gcaaatatgc acactcatgt
      841 tctcgacaat accaagcacc ggaacctcga ctttctcgaa catcacaatg ccttttttcg
      901 catcgatcaa cgcgatgtcc tgcggggtgg tgaccacgac tgcaccagtt actggaatgt
      961 tttgcgccag cgtcaattga atgtcaccgg tgcctggcgg catatcaagc accagatagt
     1021 cgagatccgg ccacaaggtt tcctgcaaca tctgcattaa cgccttgctc gccatcggtc
     1081 cacgccacac catcgcattg tcgtcagtga ccagataacc aattgaattg gttgccaggc
     1141 catgagacat aatcggtgcc atatgggtac cgtccggcga agttggacgc tgattttctg
     1201 cgcccagcat ggtgggaatt gatgggccgt agatatcggc gtccagaatg ccgactttcg
     1261 ccccttccgc cgccagtgcc agcgccaggt ttaccgccgt ggaggattta cccacgccgc
     1321 ctttaccgga actgaccgca atgatgttct tcacaccatt aatacccggc tgattcttca
     1381 cgcgttttaa ggtggcgata ttgtgcgaca gtttccagtc gatagctttc gccccagtga
     1441 tacgcagcag ttcggcgcta cattgctctt ttaactcttc aaacgcgcta tgccagacga
     1501 atggcataac cagttccaca tgcaacgtgt cgtccatcca ggcgacatgg tgcaacgctt
     1561 tcagcgtggt gaggttatgc ttcagggttg ggtgctgaaa attggccagt gtcccggcga
     1621 ccattgcgcg caaggcttct ggtgatttgg cctgggattg ttcgttcatc ccgactcctt
     1681 ttttgtatag ataaaccatc agctgatagt ttacctgaag aatatagaga agtacttact
     1741 taacattttc ccatttggta ctatctaacc ccttttcact attaagaagt aatgcctact
     1801 atgactcaag tcgcgaagaa aattctggtg acgtgcgcac tgccgtacgc taacggctca
     1861 atccacctcg gccatatgct ggagcacatc caggctgatg tctgggtccg ttaccagcga
     1921 atgcgcggcc acgaggtcaa cttcatctgc gccgacgatg cccacggtac accgatcatg
     1981 ctgaaagctc agcagcttgg tatcaccccg gagcagatga ttggcgaaat gagtcaggag
     2041 catcagactg atttcgcagg ctttaacatc agctatgaca actatcactc gacgcacagc
     2101 gaagagaacc gccagttgtc agaacttatc tactctcgcc tgaaagagaa cggttttatt
     2161 aaaaaccgca ccatctctca gctgtacgat ccggaaaaag gcatgttcct gccggaccgt
     2221 tttgtgaaag gcacctgccc gaaatgtaaa tccccggatc aatacggcga taactgcgaa
     2281 gtctgcggcg cgacctacag cccgactgaa ttgatcgagc cgaaatcggt ggtttctggc
     2341 gctacgccgg taatgcgtga ttctgaacac ttcttctttg atctgccctc tttcagcgaa
     2401 atgttgcagg catggacccg cagcggtgcg ttgcaggagc aggtggcaaa taaaatgcag
     2461 gagtggtttg aatccggcct gcaacagtgg gatatctccc gcgacgcgcc atactttggt
     2521 tttgaaattc cgaacgcgcc gggcaaatat ttctacgtct ggctggacgc accgattggc
     2581 tacatgggtt ctttcaagaa tctgtgcgac aagcgcggcg acagcgtaag cttcgatgaa
     2641 tactggaaga aagactccac cgccgagctg taccacttca tcggtaaaga tattgtttac
     2701 ttccacagcc tgttctggcc tgccatgctg gaaggcagca acttccgcaa gccgaccaac
     2761 ctgtttgttc atggctatgt gacggtgaac ggcgcaaaga tgtccaagtc tcgcggcacc
     2821 tttattaaag ccagcacctg gctgaatcat tttgacgctg acagcctgcg ttactactac
     2881 actgcgaaac tctcttcgcg cattgatgat atcgatctca acctggagga tttcgttcag
     2941 cgtgtgaatg ccgatatcgt gaacaaagtg gttaacctgg cctctcgtaa tgcgggcttt
     3001 atcaacaagc gttttgacgg cgtgctggca agcgaactgg ctgacccgca gttgtacaaa
     3061 accttcactg atgccgctga agtgattggt gaagcgtggg aaagccgcga atttggtaaa
     3121 gcagtgcgcg aaatcatggc gctggctgat ctggctaacc gctatgtcga cgagcaggct
     3181 ccgtgggtgg tggcaaaaca ggaaggccgc gatgccgacc tgcaggcaat ttgctcaatg
     3241 ggcataaacc tgttccgcgt gctgatgact tacctgaagc cggtactgcc gaaactgacc
     3301 gagcgcgcag aagcattcct caatacagaa ctgacctggg atggtatcca gcaaccgctg
     3361 ctgggccaca aagtgaatcc gttcaaagcg ctgtataacc gcatcgatat gaagcaggtt
     3421 gaagcactgg tggaagcgtc taaagaagaa gtgaaagcag ctgccgcgcc agttactggc
     3481 ccactggcag atgatccgat tcaggaaacc atcacctttg acgacttcgc aaaagttgac
     3541 ctgcgcgtgg cgctgatcga aaacgcagag tttgttgaag gttctgacaa actgctgcgc
     3601 ctgacactgg atctcggcgg tgaaaaacgc aatgtcttct ccggtattcg ttctgcctac
     3661 ccggatccgc aggcactgat cgggcgtcac accattatgg tggccaacct ggcaccgcgt
     3721 aaaatgcgct ttggtatctc cgaaggcatg gtaatggctg ctggtcctgg cgggaaagat
     3781 atcttcctgt taagcccgga tgctggtgct aaaccaggcc atcaggtgaa ataatccccc
     3841 ttcaaggcgc tgcatcgaca gcgccttttc tttataaatt cctaaagttg ttttcttgcg
     3901 attttgtctc tctctaaccc gcataaatac tggtagcatc tgcattcaac tggataaaat
     3961 tacagggatg cagaatgaga cactttatct atcaggacga aaaatcacat aaattctggg
     4021 cagttgagca acagggaaac gagttgcata tcagttgggg aaaagttggc accaaagggc
     4081 aaagccagat aaaaagtttt tcagatgctg cggcagcggc aaaagcggag cttaagctga
     4141 ttgcggagaa ggtgaagaag gggtatgtgg agcaagcgaa ggataattct ttgcaacctt
     4201 cccaaacggt aacgggctct ctcaaggtag cggacttatc caccattatt caggaacaac
     4261 cctctttcgt agcagaaacc cgtgcgcctg acaaaaatac agatgctgtt ttaccgtggc
     4321 tggcgaaaga tattgctgtc gtttttccgc ccgaagttgt acacaccacg ttaagtcatc
     4381 gccgctttcc cggagttcct gttcagcaag cagacaaatt gacccaatta cgtcgcttag
     4441 cctgtagtgt gtcgcaacgg gataataaaa cagccacatt tgacttcagc gcctgttctt
     4501 tagaatggca aaacaccgtc gcccaggcga tcagtcagat cgacggcctg aaaacaacac
     4561 agctaccatc accagtaatg gctgtactca cggcacttga aatgaaatgc acaagatata
     4621 aagtgcgtga ggatgttatg gatcagatcg tccaggaagg cggtctggaa tatgctactg
     4681 atgtaataat acaccttcaa cagattgata ttaaatggga ttatgcgaat aatgtcatta
     4741 ttattctgcc gtctggcatt gcacctgact acttggagca atattccaga tttgaattac
     4801 gcctacgtaa acatttatca ctggcggaag agtctctctg gcaaaaatgt gcacaaaaac
     4861 ttattgccgc aattccacat attccagaat ggcggcaacc attaattgct ttgttattac
     4921 ccgaaaaacc agaaattgca catgaaattg cccagcgtct actggggcaa aaaaaattac
     4981 cctcgcttga gtggttaaaa atagtggcga ctgatgagca cattcttgcc tcattagaaa
     5041 aatatcacga gccatatgcc atttttgatg attactattg tggtgcgata tggtcagcca
     5101 ccgtattaca ggagcaaggt gttgcagccc tgccccgatt tgctccctat gccgcaagtg
     5161 actactgcgc cgatgtgttg cgtcatatca atcatccgtt cgcattaaca ctgcttatac
     5221 gtgtagccgg gcatactaaa cgctgtcacg atcggatgac gaaagccatt gctgcgttcc
     5281 cacatgcagc aatggcggca ctgacggaac ttcttgggca aaaagaagag aacagttggc
     5341 gcattatgct aatgacaatg cttatctcac aaccagcact ggcagaacag gtcattccct
     5401 ggctctcgac acccgcagtt gccgtactga aatcatgcca gcaacaactg acacagccct
     5461 caaaccatgc cagcgccgat ctactgccag ccgtagtagt ctcccctccc tggctttcga
     5521 aaaagaaaaa atcgccgatt ccggtgctgg atttagcgcc attaggcatt gagccaatct
     5581 gttatctgac agaagaaatc agtaatcaac ttttggcgaa atatatctgg tattcaaaac
     5641 acatcacggt tagccatgaa gaaagtacta ccaacctgtt ggcaaggatg ggttttcaac
     5701 gacggatcgc tggtacatat attaaagctc ccgaagcggt agttgaggca tggctaaatg
     5761 aagattattc aaccttacta agtgaattta aggtgtttca ttcacctacc gggcattatt
     5821 ggcagttggg gattttgaca acattgccgc tggagaaagc agtaaaagca tggaatgccc
     5881 ttaccctatc tccacatacc gataccgaat acgccatgtt acattttgga ctcaaagggt
     5941 tacctgggtt agtaaactca cttgcacgct atccacaaga agccttgccc atcacgaatt
     6001 acttcacagc gagtgagctg gctcctgccg tcgcccgtgc cttcaacaaa ctcaaaacgc
     6061 tacgcgaaaa cgcccgtagc tggctgttga aatacccgga acatgccctt accggcctgc
     6121 tgcctgcggc gctcggcaaa gccggtgaag cacaggataa cgcccgcgct gccttgcgta
     6181 tgcttaccga aaacggtcat cagccattac tgcaagaaat cgcccgacgt tataaccagc
     6241 cggaagtaac cgatgcggtg aacgctctgc ttgcgctcga tcccttagat aatcacccga
     6301 caaaaatccc cactcttccg gccttttatc aaccatcgct ctggacgcgc ccggtattaa
     6361 aagcaaacgc ccaatcactg ccagatagcg ccctcctcca cctcggtgaa atgctccgct
     6421 tccctcagga agaggttctg tatccgggaa tattacaggt gaaagacgcc tgtaccgccg
     6481 actcactggc tgaatttgcc tgggatctgt ttaacgcctg gcagaccgct ggcgcgccgt
     6541 cgaaagagag ttgggcgttc actgcgttag gcgttctcgg taacgatgac accgcccgca
     6601 aactgacgcc attaatccgc gcctggcctg gtgaatccca gcataaacgc gccaccgttg
     6661 ggttggatat tctcgctgct atcggtagtg atatcgccct tatgcagctt aacggcatcg
     6721 cccagaaact gaaattcaaa gcattacagg agcgggcaaa agaaaaaatt gccgacattg
     6781 ccgagagccg cgaactcacg gtggcggagc ttgaagatcg gttagcaccg gatctcggtc
     6841 tggatgataa cggttcgctg ctgctggatt ttggcccacg gcagttcacc gtcagctttg
     6901 atgaaacctt aaaaccgttt gtgcgtgatg tttccggcag ccgcctgaaa gacctgccca
     6961 aaccgaacaa aagcgatgat gaaacgcggg cgaacgatgc ggttaaccgc tacaaattgc
     7021 tgaaaaaaga tgcgcgtacc atcgccgccc agcaggtagc aaggctggaa tccgccatgt
     7081 gcctgcgccg ccgctggtcg ctggaaaact tccagctctt cctggttgag catccgctgg
     7141 ttcgtcactt aacccgccgt ctgatttggg gcgtttatag cgccgaaaac cagctactgg
     7201 cttgctttcg cgtagcagaa gataacagct acagcaccgc tgacgatgat cttttcaccc
     7261 tgccggaagg cgatatctct atcggcactc ctcacgttct ggaaatatca ccaacggatg
     7321 ctgccgcctt tggtcagctt tttgccgact acgaactgct accaccgttc cgccagctcg
     7381 accgtaacag ctacgccctg acagaagccg agcgcaatgc cagtgaactg acccgctggg
     7441 caggcagaaa atgcccgagt ggtcgggtca tggggctggc gaataaaggc tggataaagg
     7501 gcgaaccaca ggatggaggc tggatcggat ggatgatcaa acctttgggt cgctggtcgt
     7561 taatcatgga aatcgacgaa ggttttgcgg tgggtatgtc accagccgaa cttagcgctg
     7621 agcagctctt aagcaagctg tggttatggg aaggcaaagc ggaaagctat ggctggggga
     7681 gtaattcaac acaggaagcg cagttctccg tactcgatgc catcaccgcc agcgagctaa
     7741 ttaacgatat tgaagccctg tttgaataag gaaagagcat ggacaaggaa ttaccgtggc
     7801 tggcggataa cgcccaactg gaactgaaat ataaaaaagg caaaacacca ctcagtcatc
     7861 gcaaatggcc gggcgaaccg gtgcctgtta tcactgaaag catcattcag acactgggtg
     7921 atgaattgct acaaaaagct gagaagaaaa aaaacattgt ctggcgttat gaaaattttt
     7981 cactggagtg gcagtccgcc attacgcagg ccatcaactt gatcggcgaa cacaaaccct
     8041 cagtcccggc ccgcacaatg gcggcactag cctgtatcgc gcaaaatgac aaccaacagt
     8101 tgctcgacga aatcgtccaa caagaggggc tggaatatgc gactgaggtg gtgattgcac
     8161 gccagtttat tgcacggtgt tatgagagtg atcctctggt agtcacattg cagtatcaga
     8221 acgaggacta tggctatggt tatcgctcag aaacctataa cgaattcgat ctccgactgc
     8281 gtaaacacct ctctctggca gaggaaagct gctggcagcg ttgcgccgac aaactcattg
     8341 ccgcactgcc aggaataacc aaagttcgcc gcccttttat tgcgctgatc ctccccgaaa
     8401 aaccagaaat cgccaatgag ttggtaagcc ttgaatgccc gcggactcat tttcattcta
     8461 aggagtggtt aaaagttgtt gctactgacc ccaaagcggt gagaaaactc gaacgctact
     8521 ggagccagga tatatttagc gatcgagaag ccagctacat gtcgcatgaa aaccacttcg
     8581 gctacccgcg atcgcagcgt caatgaaatg agtgctgcgc taaagcgtcg cttcgatttt
     8641 gaaactgtct ttccgattat ggatttcgcc caggagctgg agcttgtcgc cagtgcttcg
     8701 gcgcgtttgc tggcccatag cggtattccg tataaagtgc ctgacgcggt actggagtta
     8761 ctggtccgca ccttccgcga tctgcgtgcc aacggcgaaa agaaaacgtc aatggataca
     8821 ctaacagcga ttatgtccac cgctgaagcc gtgaacgtgg cccacgccgt aggcgtccgc
     8881 gcctggtttt tagcgaatcg cgcaggtgag ccagccgacc tggtggactg cattgccgga
     8941 accatcgtca aagataacga agaagatcgc gctcgtctgc gccgttactt cgaacaacga
     9001 gtcgccacac ataaagaagc tcactggcag gcttattatc aagcccgcca ccgcctgccg
     9061 tgaggaaaga tgcatgagcg agccgttaat tgtcggcatc cggcatcata gtccggcctg
     9121 cgcccggctg gtgaaatcgt taatcgaaag ccagcggcca cgatacgtat tgattgaagg
     9181 cccggctgat tttaatgacc gggtagacga actgttttta gcccaccagc ttccggtagc
     9241 tatttacagt tattgccagt atcaggacgg tgcagccccc gggcgtggtg cctggacgcc
     9301 atttgctgaa ttttcgccgg agtggcaggc gctacaagcc gcacgtcgta ttcaggcaca
     9361 aacttacttc atcgatttgc cttgctgggc gcagagtgaa gaagtggacg attcgcctga
     9421 tacgcaagag gaaagccagg ccttgctgct gcgtgccacc cgcatggata acagcgacac
     9481 cctgtgggat cacttgttcg aagatgaaag ccagcaaact gcattaccct ctgcactggc
     9541 gcactatttt gcccaactgc ggggcgactc ccccggagat gctctcaatc gtctgcgcga
     9601 agcctttatg gcccgctgga ttggatgggc gatgcagcaa aataatggcg acgtgttagt
     9661 tgtctgcggt ggctggcacg ctccggtact ggcaaaaatg tggcgcgaat gcccgcagga
     9721 aattaacacg ccagaattgc cgtcgctggc agatgccgtt acaggttgtt atctcacccc
     9781 ctacagtgaa aagcgccttg atgtgctggc aggatacctt tccggaatgc ctgccccggt
     9841 ctggcaaaac tggtgctggc agtggggctt acagcaggcg ggagaacaac tgctaaaaac
     9901 ggttctcacc cgtttgcgcc agcacaagtt gcctgcttcg accgcggata tggctgccgc
     9961 tcatctgcat gcgatggcac tggcacagtt gcgcggtcat acactaccgt tacgcactga
    10021 ctggctggat gccatagcag gatcgctgat taaagaagcc ctgaatgcgc cgttgccgtg
    10081 gagctatcgc ggcgttattc atcccgacac cgatccgatt ctgctaacgt tgatagacac
    10141 attagcgggt gacggattcg gtaaacttgc cccttctacg ccacaacctc ctctgccaaa
    10201 agatgtcacc tgcgaactgg aacgtaccgc aatctccctt ccggcggagc ttaccttaaa
    10261 tcgctttaac cccaatggac tagcgcaaag tcaggtgtta catcggctgg caatactgga
    10321 gatcccaggg attgtgcgcc agcagggaag cacactgaca cttgcaggca acggtgaaga
    10381 acactggaaa ttaacccgcc cgcttagcca gcatgcggca ttgattgagg ccgcctgctt
    10441 tggtgccaca ctccaggaag ccgcacgcca taaattagaa gccgatatgc tggacgcggg
    10501 tggaatcggc agtatcacca catgtcttag ccaagcggcg ttagcgggtc tggcgtcctt
    10561 cagtcaacaa ttactggagc aactcacatt attaatcgcc caggaaaatc aatttgccga
    10621 aatgggccag gcgctggaag tgctatatgc cttatggcgg ctggatgaaa ttagcggtat
    10681 gcaaggcgcg cagatattac aaacgacgtt atgcgcggct atcgatcgca cgctgtggct
    10741 gtgtgaatct aacggcagac cggatgaaaa ggagtttcac gctcacctgc atagctggca
    10801 agcgctttgc catattctgc gcgatctaca tagcggcgtt aatttatccg gcgtttcgct
    10861 ttctgcggcg gtagccttac tggagcgacg cagtcaggcg attcatgccc cggcgctgga
    10921 tcgcggcgcg gttcttggcg cactaatgcg tctggaacat cccaacgcca gtgccgaagc
    10981 ggcgctgacg atgctggcgc agttatcccc ggcacaatcc ggcgaggcgc tgcacggttt
    11041 gctggcatta gcccgtcatc aactggcctg tcagccggca tttatcgccg gtttcagcag
    11101 tcatttaaat caactaagtg atgctgattt tatcaatgcc ctgcccgatt tacgcgcggc
    11161 gatggcctgg ctaccaccac gagaacgcgg gacactggcg catcaggtgc ttgagcatta
    11221 tcaactggcg caacttcccg tttcggcgct gcaaataccg ttgcattgtc caccgcaagc
    11281 cattgcacat catcaacaac tcgaacagca ggcactggca tcgctgcaac actggggagt
    11341 tttccatgtc tgaactgaac gatcttctga ccacccgtga gctacaacgc tggcgattaa
    11401 ttcttggcga agcggcagaa acgacgcttt gtgggctgga tgacaacgcc cggcagatag
    11461 accacgcgct ggagtggctg tacgggcgcg atcctgaacg gctccagcgt ggtgaacgtt
    11521 ccggtggatt aggtggctca aatctcacca cccctgagtg gatcaacagt attcacacgc
    11581 tgtttccgca acaggtgatt gagcggctgg aaagcgatgc cgtactgcgc tacggcattg
    11641 aagatgtggt gacaaatctc gacgtgctgg aacgtatgca gccttccgaa agcctgctac
    11701 gcgccgtttt gcacaccaaa catctgatga atcccgaagt actggctgcc gcccgccaga
    11761 tagtgcgcca ggttgttgaa gaaattatgg cgcgactggc aaaggaagtt cgccaggctt
    11821 tttctggtgt ccgcgatcgc cgtcgccgtt catttattcc actggcgcga aactttgatt
    11881 tcaaaagtac tctgcgcgcc aacctgcaac actggcaccc gcaacacggc aagttgtata
    11941 tcgaatcccc ccgctttaac agccgcatta aacgccaaag cgaacaatgg caactggtct
    12001 tactggttga tcaaagcgga tcgatggtcg attcggtgat ccactctgcg gtgatggcgg
    12061 cctgtttgtg gcagttaccc ggcattcgta cccatctggt ggcgtttgac acaagcgtcg
    12121 ttgatctcac ggcagacgtt gccgatccgg tagagttatt aatgaaagta cagttgggcg
    12181 gcgggaccaa tatcgccagt gccgtggagt atggtcggca acttattgaa caaccagcga
    12241 aaagcgtcat tatcctcgtg agcgattttt acgaaggggg ttcatcatca ttactgacgc
    12301 atcatgtgaa aaagtgtgtc cagagcggca tcaaagtgct gggactggca gcgctcgata
    12361 gcaccgcaac accttgctat gaccgcgata cggcccaggc gctggttaat gtcggcgcac
    12421 aaatagccgc catgacgccg ggcgagctgg catcatggct tgcggagaat cttcagtcat
    12481 gaattcacta cgtccggaat tattagaact gacaccgcag gccctgacgg cgttaagcaa
    12541 tgccggtttt gttaagcgca gtcttaagga actggaaaat ggcaacgtcc cggagatcag
    12601 ccatgagaac gacgctttaa tcgccacctt cagtgacggt gtccgtaccc agctggcgaa
    12661 cggccaggca ctgaaagagg ctcagtgcag ttgcggggcc aacggtatgt gccgtcatcg
    12721 cgtgatgctg gtgttaagtt atcaacgact ttgtgccacc actcagtcta cggaaaaaga
    12781 agaagagtgg gatccggcaa tctggctgga agaactggct acccttcccg atgctacccg
    12841 caaacgcgca caggcgctgg tcgctaaagg catcaccatt gagttgttct gtacgccggg
    12901 cgaaattccc tctgcccgct taccgatgag cgatgtgcgt ttttattccc gtagcagcat
    12961 tcgtttcgcc cgttgtgatt gtattgaagg cacactttgc gaacatgtcg tactggcagt
    13021 acaggccttt gtctaggcaa aagcgcagca agcggaattg actcatttaa tctggcagat
    13081 gcgcagcgaa cacgtcacat catctaacga tccgtttgcc aacgacgagg gtaacgcgtg
    13141 tcgtcaatat gttcagcaat taagccaggc attatggctg ggcggcatca gccagccgct
    13201 tattcactac gaggccgctt ttagtcgcgc gcagcaggcg gcggaacgct gcaactggcg
    13261 atgggtgagt gaatcactac gacagctacg ggcaagcgtt gatgccttcc acgcccgcgc
    13321 cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca gtcgattaaa
    13381 ttgcgcacaa gagatggccc ggcgcgacag tattggtgaa gttcctcctg tgccgtggcg
    13441 cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc ggctggtgtc
    13501 tttaggtatg cgttgctggc aggatattga gcattatggt ttaggcatct ggtttaccga
    13561 tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg aacaggaaaa
    13621 ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactag cgggcgggca
    13681 aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag ctacccgcaa
    13741 ccgcttaagc agcgttgtgc cgctgtcgcc tgatgcctgg caaatgttga gcgcgccgtt
    13801 acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc ccgcctgcat
    13861 acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt gtatttcgct
    13921 cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg aaggtgaaga
    13981 taatctcctg acgttatccc ttccggcatc tgcctgttct ccttttgccg ttgaacgcat
    14041 ggcggcgctt ttgcaacaaa cagacgaccc cgtgagtctg gtttctggct ttgtcagctt
    14101 tgtagagggg caattgacac tggaaccacg ggtgatgatg acaaaaaccc gcgcctgggc
    14161 gctggacgca gaaactgcgc ctgtggcacc gctaccttct gccagcgttt tgcctgtgcc
    14221 gtctaccgct catcagttgc tgatgcgctg ccaggcgtta cttattcaac tgctccataa
    14281 cggctggcgc tatcaggaac agagcgctat tagtcaggca gaattgctgg cgaatgacct
    14341 ctccgcggtc ggtttttatc ggctggcaca tgtgttggga caatttcgtg atacagaaag
    14401 cgaggcacgg gtagaagcaa tgaataacgg tgttttactt tgcgaacaat tattccccat
    14461 gcttcagcaa caaggatgaa atagtgcttt ttactaagag ttctactcca gttccggact
    14521 gctcacgcca cggtattagg catatcctat atagcccctg gtgagagtca ccagttcctt
    14581 gattaaataa aatggagttt tacatgaagg ctttcaataa gctgttttcc ctcgttgttg
    14641 catctgttct ggttttctct cttgctggct gcggtgacaa agaagaatcg aagaaattca
    14701 gcgccaatct gaacggcact gaaattgcca ttacctatgt ctacaaaggt gacaaggtgc
    14761 ttaagcaatc ttctgaaacc aaaattcaat ttgcctccat tggtgcaacc accaaagaag
    14821 atgctgccaa gacacttgag ccgttaagcg ccaaatacaa aaacatcgcg tgtgttgaag
    14881 aaaaattaac ctataccgat acctacgcgc aggaaaacgt gactatcgat atggaaaaag
    14941 tggattttaa agccctgcag ggtatttcag gaatcaacgt ttctgctgaa gatgccaaaa
    15001 aaggtatcac tatggcgcaa atggaactgg tgatgaaagc cgctggtttt aaagaagtga
    15061 aataatcggt tggcggtcat gctctaaaca tgaccgccaa ttttttagcc ttttttcaca
    15121 tgctggcgcg ctgccagtcc acgcagaaaa taacgtaaaa attgatcgcc gcattcgcgg
    15181 aagtttttat gatccggtgc gcgcatcatc gctgtaattt ccggcatcga aacgcggaac
    15241 tgctgttcgg tgaggatagc cagaatgtca tcggttttca gcgaaaacgc gatgcgtaat
    15301 tttttcagca cgatgttgtt attaatgcga cgttccggct ccagtgccgg agcagactca
    15361 tccttgccgc gtttttcata aatcaggcca ttgaggaatg acgacaaaac aatgtccgga
    15421 caacgctgaa aaccctcttc gtcttcttta cgtagccaga cggcgatctg ttctgcggtg
    15481 gcttcgacat tacccagcgc cagaatacgc accaggtcat tattattggc tttcaaaatg
    15541 tagcgcacgc tgcgcagaat atcgttactt agcatgaggc cttcaggtgt tgatgaggca
    15601 aaaagccatt ttagcagtct tttacaggcc aatcgcctct tttaagcttt tcagataacg
    15661 gcggctgacc ggcacggtta agccattacg caaaatcaac tcggcctggc cgttatcttc
    15721 cagacgaatc tcctgtaaat gcgcgaggtt aaccagatac tgacgatggc agcgcagtag
    15781 tggtgtacga ctttccaggg tacgtaatgt caattcggta aagccctctt tcccttcgtg
    15841 gctggtaacg tagacaccgc tcatccgact gctgacaaat gccacatctt tcatttgcag
    15901 caaataaatc cgactatgcc ccgtacaagg gataaatttc agcgcctgtt gattttccgg
    15961 taacagcgaa acatcctgct tgctgcgctc ctgacgcaat cgcgccagcg ttttctccag
    16021 tcgcgcttca tcaattggct tcagcagata atcaaaggca tgttcttcaa aggctttaat
    16081 tgcgtattcg tcaaacgcag tgagaaaaac aatatacggg cgatgttccg ggtcaagcat
    16141 ccccaccatt tccagaccac tgatgcgcgg catctggata tcgagaaaca gcacatccgg
    16201 gcgcagttta tgcaccgcgc cgatcccttc cacggcgttt gaacactctc caacgatttc
    16261 aatatcgctc tgctcctgca aaaatacacg caggttctcc cgtgctaacg gttcatcatc
    16321 gacaattaag actttaatca tgcctcgtcc ctccatggta gtcgtaacgt tattcgggtg
    16381 taactatcag gctcacaggc gacgcttatt ccatagtcat cgccaaaccg ttcacgtaaa
    16441 cgcttatcca ccagattcat ccccagccca ctggcattgg ttaccggttg atacaaaccg
    16501 gcattgtctt cgatctccag catcaaatgt tgcccctcac gtcgggcgct gattgccact
    16561 cgccctgtat ccagcagttg tgatgtccca tgtttaatgg cgttttccac tatcggttgc
    16621 agggtaaacg cgggcaattg ctgctgggat aattcttgcg gaatagcaat gttgacctgc
    16681 aaccgcgact ggaagcgcgc cttttcaatt tgcagataag cgttcacatg ttcaatttcg
    16741 tcggcgagag taacaaactc cgaaggccgc tttaagtttt tgcggaaaaa agtggaaaga
    16801 tactgcacca gctggctggc ctgttcgctg tcgcggcgga tcaccgcttt aatggtgtta
    16861 agcgcattaa acaaaaaatg aggattcacc tgggcgtgaa gcagtttgat ttctgactgg
    16921 gtgagcatcg ctttttgccg ctcatattgc ccggcaagaa tctgcgccga aagcagttgc
    16981 gcaatcccct cgcccagcgt gcggtttatt gaactgaata aacggttttt ggcttcatac
    17041 aatttgatgg tgcccatcac gcgctgattt tcgccacgca acggaattac cagcgtcgac
    17101 cccagtttgc attgcggatg caaagagcaa cgataaggta cttcgttgcc atcagcgtag
    17161 accacttcac cggtttcaat cgcttttaag gtgtaagtcg aagaaatcgg tttgccgggt
    17221 aaatggtggt cgtcaccaat tccggtaaag gccagcaatt tctctcgatc ggtaatcgcg
    17281 actgcaccaa tatccagctc ctgatacagc acctgtgcca ctttcatgct gttcacttcg
    17341 ttaaacccct ggcgcaaaat gccttccgtc gaggcagcca ctttcagcgc agtggcagaa
    17401 aaagccgaag tgtatttttc aaacatcgcg cgtttatcga gcaatatacg cataaacagc
    17461 gccgcgccga cggtattggt gaccatcatt ggtgcagcaa tattactcac cagacgcacc
    17521 gcatcttcat aaggtcgggc gatcgccagg atgatcaaca tttgcaccat ttcagcgacg
    17581 aacgtgacgg caccggcggt aatggggtta aagactttat cagtgcgccc gcgacgaatc
    17641 aggatgctgt gtaccaggcc gccgagcaat ccttcaacga tggtcgagat catgcaactt
    17701 agcgcggtca tgccccccat cgaatatcga tgtaacccgc cggtcaggcc aaccagccca
    17761 ccgacgaccg gaccgccgag taagccgccc attaccgcgc ctatcgcacg ggtattggca
    17821 atagaatcgt caatgtgcaa cccaaaccag gtgcccatga tgcagaagat ggaaaagacg
    17881 atgtagcaga gaaatttatg cggcagacga accgtgacct gcattaacgg tatgaataat
    17941 ggcgttttac tcattaacca cgcaatgact aaaaaaacgc acatctgctg aagcagcagc
    18001 aacaccagat taaaatcgta catacccgca aaccacactt ccctttaaaa cgcgtaacat
    18061 acattgcctg cgtttaactt tctttgaact cttgcagaaa aatgagaatt cgtgagtacg
    18121 atcactcaaa atcgcctggc aaaaataaaa tcaccctata gatgcacaaa aaacgggcaa
    18181 aactacctgg ttcgcaaaac tgcgtctaaa gttaaaccgg gacctcgcga gcaagggtga
    18241 gacgatggcg ctttacacaa ttggtgaagt ggcgttgctt tgtgatatta atcctgtcac
    18301 gttacgcgcg tggcagaggc gttacggatt gctgaaaccg caacggacag acggcggtca
    18361 tcggctgttc aacgatgccg atattgaccg gatccgcgag atcaaacgct ggatcgacaa
    18421 cggcgtgcag gtcagcaaag ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa
    18481 cggctggcgc gatcagcaag aaacattact gacttacctg caaagcggca atctacatag
    18541 cctgcgaacg tggatcaaag agcgcggtca ggattacccc gcccagacac tcaccacaca
    18601 tctgtttatt cctctgcgcc gacggcttca gtgccaacaa ccgactctcc aggcgctgct
    18661 ggcgatcctc gacggcgtac tgatcaacta catcgccatt tgtctggctt cggcacgtaa
    18721 aaaacagggt aaagatgcgc tggtggttgg ctggaatatt caggatacca cccgtctgtg
    18781 gctggagggc tggattgcca gtcaacaagg atggcgcatt gatgtcctcg cccactcgct
    18841 caatcaacta cgccctgaac tattcgaagg ccgtacattg ctggtgtggt gcggtgaaaa
    18901 tcgaacctcc gcccaacagc aacaactcac cagttggcag gaacaaggcc atgatatttt
    18961 cccactcggc atttaatgat tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa
    19021 cataaggagt gcattatgcg catagctaaa attggggtca ttgccctgtt cctgtttatg
    19081 gcgttaggcg gaattggtgg cgtcatgctc gcaggttata cctttatttt gcgtgctggc
    19141 taagcgcctg caccagcctt tcaaacaggc ggtctgcgat gatcgccgcc agtgccacca
    19201 gtaacgcccc ctggatcaca tacgcggtat taaatccgct aagcccgatg atgatgggcg
    19261 tacccagcgt gctggcccct accgttgagg cgatcgtcgc cgtaccaatg ttgataatca
    19321 ccgaagttcg cacgcccgcc agaatcaccg gagccgccag cggtagctcg accttacgca
    19381 gtcgctgacc acgactcatt cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg
    19441 ctcccagccc ggcaagtgtc gcctgcagga cgggcagcac accgtaaagg atcaaggcga
    19501 taatcgctgg ttgcagacca aagccgatca ccggaacggc gatcgccagc actgcgacgg
    19561 gcggaaaagt ctgtccaacg gcggcaatag tttccaccag tgggcgaaat tccgcgcccc
    19621 acgggcgagt gacagcaatt ccggcaccag taccaatgat caccgcaaac aaactcgaaa
    19681 ttcccaccag ccagaaatga gccagtgcca gagctgcaaa actttcttgc tgataaacgg
    19741 gtcgtggcag ttgtgggaac aaggcagcaa acagcggctg gctgtaaggc agccagaaaa
    19801 tcagtgccac aaacaaagca atgagccaga acagcggatc gcgcaacatt ttcatacgct
    19861 tacgcctcct ccagcagatc ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc
    19921 atcaccggca gcacctcgca tccccgcgcg acaaacaggg agagcgcatc gcgtagcgtc
    19981 atctcttctg ccagtgcctc accttctgcc cgttcttcgc gacgcacgta atccgccaca
    20041 ctacgtaacg aaagcaggcg cacccccagt tcactacgtc caaaaaactg gcggacaaaa
    20101 tcattcgccg gacgagtcag catcgtcagc ggattcccct gctgcaccac ttcaccgtga
    20161 tccatcaata ccagatgttc tgccagcctt agcgcctcat caatatcatg agtgaccagc
    20221 acaatagtgc gccccagcaa acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc
    20281 gttaccgggt ccagtgcgcc aaaaggttca tccatcagta agacttgcgg atcggcagcc
    20341 agtgcgcgcg ccactcccac acgttgctgc tgaccaccgg aaagctgatg cggataacgc
    20401 tcacgcaaat ttgactccag ccccagtagc gccattaatt cgtcgatacg atcgtcaatc
    20461 cgtgcccgcg accatttttg taattgcggc acggtagcga tgttttgcgc cacgctccag
    20521 tgggggaaca ggccaataga ttgaatggca tagcccatcc ggcggcgcaa ctccagtact
    20581 ggcagcgagc gaatttcttc tccggcaaag cggatcacgc cgctgtcatg ctccaccagg
    20641 cggttgatca ttttcagggt ggtggatttg ccggagccag atgtgccaat cagcaccgaa
    20701 aaactccctt cctgaaaatt gagattgaga tcgttaacgg ctttttgtgc gccgaacagt
    20761 ttgctgacat ggctaaattc aatcattacg tttcaccttc agcagtgcga taagtaaatc
    20821 gaacagcgcg tcggtaagca ccgccagaac aattaccggg atcaccccca gcaacactaa
    20881 atcaatggcg ctgcttagca gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat
    20941 taacgccgca atcaccgcca tgcctacagt ttgcaccatc accacccgca ggctgcgcag
    21001 aaataccggt aacgccagcg gtaactgaac atgcaggaaa cgctgcgccc cgctcatccc
    21061 catcgctctg gcgctctcca gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc
    21121 gcgcaccagc ggcagcaagg catagagcac cagcgcaatc agtgcgggtg tcattccggt
    21181 tcctgctatg ccgagcttcc ccagccacgg aaaggccgta accagcgcgg caagcggcgc
    21241 aatcaacagg ccaaagagcg ccaccgaagg cacggtctga atgacattga gcagagaaaa
    21301 aattgccccc tgccgcgcag tggaaaagta gcaccagatg cccaacggca caccaatcac
    21361 taacgcaggc agcaccgcac caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa
    21421 cacatcctga cggttggcgt attctttcat tagtgagaga tcgttaagcg tgccggagta
    21481 cagcaaccac agcggaataa tggcaatctg catatgcaac aaccagcgcc acagcggatg
    21541 cgtggagatt cggcggatgg catcgctaca ggccagcaat gccagcgccg cagccagcca
    21601 gaaaccactg ccgaggctgg tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc
    21661 cgcctttcca gctccccaca ccagcaatac gaagacgaat tgcaccagaa tgagtgcaaa
    21721 aatgctgcct tttttaccgg gaataaaaca ggctgtcagc caggcgcaac caacgcccac
    21781 cagcatccag atcgtttgcg gccacagctg ccagagatgt cgcccttcac ccgagactaa
    21841 acgattaggc gcgtaactga taaacggcag cgccgctgcg attaccgtca gcaacaacag
    21901 cagcgccaga acaggattaa tacgtagata agtcacggga aattacttcg cccacccttt
    21961 ttgtttcagg tagtcggcag ccactttttt ggcatccagt ccttccactg caatgctggc
    22021 attcagttgc tgcaatgttt ttgcatcgag gctggcgaag actggctgta gccactgtgc
    22081 catttgcgga tactctttca gcaccgactc acgcaccact ggtgcaggcg cgtagatagg
    22141 ttgcacgcct tgcggatcgc ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt
    22201 gccgtaagcc attgcggcat taacgccaga agtttgctgg gcagcggctt taatcgttac
    22261 cgccgtgtcg ccgccagcca gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc
    22321 tttttcaaac gcgggtaacg catcggcgcg ttcgataaac tctgccgagg ctgccagttt
    22381 gaaggtgccg ccctctttca gataacgact caggtcagca agcgaagtga gtttgttttt
    22441 ctctgccaca tcctgacgca cggcgatggt ccaggtgtta ttcgctggtg cgggcgtcag
    22501 ccagattaac ttgttttgct ctgcatcgag ttttttgact ttctcgtaac cttgctgcgc
    22561 gtttttccat gccgcatcgt tttcatcttt aaagaagaaa gcgccattgc cggtatattc
    22621 cggatagata tccagttcac cggaagtaat cgccccgcgc accacaggag tcgttcctag
    22681 ttgcacttta ttgaccgttg gtacgccgtg gctttcgagt acttgcaaaa tgatattgcc
    22741 gagcagcgcg ccttcggtat cgatttttga accgacttta acgggggaag ccgcgtgcag
    22801 cggcaggctc acggctgcca acaaaaccag tgaacctgcc cagacctttg agagtggcat
    22861 gatgctttcc tcattctttt actgttgttt tcagcgaatt aagagaaaag catagttgat
    22921 aatgacagag ttagcctgag cggaaggatt cagttgcaga atcagataaa tacttaaagg
    22981 gcatattttg cccggcgttt cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa
    23041 gggcgaaaat tcagcaagtt aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc
    23101 ttactaaagc attacagcaa ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg
    23161 ataaagacat tgaacttgcc aggctcggcg tcatatttca tctgttgatt ccagaacttc
    23221 agcgcctcaa tatcaatcgg gaagctgacg gtctgagttt cgcccggctt cagggtgatt
    23281 ttctcaaagc ctttcagctg cttcaccggg cggctcatag aagccgtcac atcctgcaag
    23341 tacatctgca ctaccgtggc cccttcgcgc ttaccggtgt tcgtcacctg cacgctggcc
    23401 gtcactttgc cgtcacgctt catggtcggc gcagaaagtt tcacatcaga gacggtgaaa
    23461 gtggtatagc tcagaccata gccgaacgga taaagcgccc cgttagcttc atcaaaataa
    23521 cgcgaagtgt atttgttcgg cttgtcggca ttatacggac gaccggtgtt cagatggctg
    23581 tagtacaccg ggatctgccc gacagaacgc gggaaggaca ttggcagctt gccggacggg
    23641 ttgtaatcgc caaacaatac atcggcaatt gcattaccgc cttcagtccc cgcaaaccag
    23701 gtttccagaa tcgcatcagc ctgctgatct tctttcacca gcgccagcgg acgcccgttc
    23761 atcagcacca gcaccagcgg tttaccggtg gctttcagcg ccgcaatcaa gtcacgttgg
    23821 ctttgcggaa tagtgatatc ggtccggctg gaggcttcgt gcgccatccc ctgtgcttca
    23881 ccgactacag ccaccaccac atcagattgt ttcgccgtct gcaccgcttc atcaatcatc
    23941 tcttgcggcg aacgcggatc gactttgacc gcttcttcat actgattcag gaaatcgata
    24001 atgcctttgt cactggtaac gttcgcccct ttggcataca gcactttacc gttttcaccg
    24061 acggaatttt taatcccggt cagtacggtc acggattgat cggcaacacc ggctgcggac
    24121 cagctgccca tcacgtcacg tttactgtcc gccagtggcc caaccaccgc aatggtggcc
    24181 gattttttca gcggtaacgt ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc
    24241 acttcacgcg cttctttacg gtgcaggcgg ctttcggcat tggtatccac cgggtcagac
    24301 tctttcggcc ccaaatggct gtatgggtcg ttaaacaacc ccatatcata tttaacgttc
    24361 agtacatggc gggcagcatc gtccagctct gccatggtca ctttgccgga tttaatcaac
    24421 ccaggcagat acttcgagta gtactcgtcg ctcatactca tgttgattcc ggatttcagc
    24481 gccacgcgca ccgcatcttc cgggtctgcc gccgtgccat gtttaatcag ctctttgatt
    24541 gcaccgtgat cggaaacggt gatgccttta aagccccact ggtcgcgcag aacatctttc
    24601 agcagccagg aatcggaggt ggctggcgtg ccgttcagcg agttcagcgc caccatcacc
    24661 gcgccgctgc ctgcgtccag ccccgctttg tacggcggca tataatcatt aaacaggcgc
    24721 tgcggactca tatcgacggt gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca
    24781 aagtgtttga cgctggtcat caccgagtag cgatctgccg ggcttttacc ctgcatcgct
    24841 tccaccatgg ttttacccat tgttgaggtg agatacgtat cttcgccaaa accttcggaa
    24901 gcacgtcccc agcgcggatc gcgcgagaca tcgaccatcg gtgcccaggt catattcagg
    24961 ccatcatctg ccgcttcata agcagagaca cgtccgaccg ttttcactgc atcgaggtta
    25021 aaagacgagg ccagaccgag gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg
    25081 taagcaaaga aaagaggaat tttcaggcgg cttaattcca tcacctgatc ctgcatggcg
    25141 cggatatcct gacgggttac ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc
    25201 tcgcggatcg cctctttcgg gttatccggg ccgacgctga ttaagcgcag ctgaccaatt
    25261 ttctcatcaa ctgtcatttt cttaagcagt tcggtgacga acgcatcccg cgcttcgggc
    25321 gttaatggat ggttgccgaa taaatcatcc gccagtgctg gctgcagggc cagactcacc
    25381 gcgattccta ctgaacatag ccatttcata tggatttttc ttcctctgtt gcccgacgag
    25441 gcagcgaaaa tggtgcaaaa accgtagttt gccataagca tgatggagag agaaaaagaa
    25501 tgctcagttt attgtctgaa ttttcaaaat attcactcgc tgaattgtca tacaaggcgc
    25561 tattctagtt tctgatattt tttcgccacc acaaggagtg gaaaatgtct tccatgacaa
    25621 caactgataa taaagccttt ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc
    25681 tcaccgatcc cgcaaaaacg gcccgctatc gcaagggctt ccgttctggt cagggcgacg
    25741 cgctggctgt cgttttccct ggctcactac tggaattgtg gcgggtgctg aaagcctgtg
    25801 tcaccgccga caaaattatt ctgatgcagg ctgccaatac aggcctgacc gaaggatcga
    25861 cgccaaacgg taacgactat gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca
    25921 agctgcacgt tcttggcaag ggcgaacagg tgctggccta tccgggcacc acgctctatt
    25981 cgctggaaaa agccctcaaa ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt
    26041 gtataggcgc atcggtcatc ggcggtattt gtaacaactc cggcggctcg ctggtgcaac
    26101 gtggcccggc gtataccgaa atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga
    26161 cgctggtgaa ccatctgggg attgatctgg gcgaaacgcc ggagcagatc cttagcaagc
    26221 tggatgatga tcgcatcaaa gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt
    26281 atgactatgt ccaccgcgtt cgtgatattg aagccgacac gcccgcacgt tataacgccg
    26341 atcctgatcg gttatttgaa tcttctggtt gcgccgggaa gctggcggtc tttgctgtac
    26401 gtctagatac cttcgaagcg gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc
    26461 cggaagtgct gaccgaaatc cgccgtcata ttctggctaa cttcgaaaat ctgccggttg
    26521 ccggggaata tatgcaccgg gatatctacg atattgcgga aaaatacggc aaagacacct
    26581 tcctgatgat tgataagtta ggcaccgaca agatgccgtt cttctttaat ctcaagggac
    26641 gcaccgatgc gatgctggag aaagtgaaat tcttccgtcc gcattttact gaccgtgcga
    26701 tgcaaaaatt cggtcacctg ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg
    26761 ataaatacga gcatcatctg ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat
    26821 cgtggctggt ggattatttc aatcaggccg aaggcgattt ctttgtctgt acgccggagg
    26881 aaggcagcaa agcgttttta caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg
    26941 cggtgcattc cgatgaagtc gaagacattc tggcgttgga tatcgctctg cggcgtaacg
    27001 acaccgagtg gtatgagcat ttaccgccgg agatcgacag ccagctggtg cacaagctct
    27061 attacggcca ttttatgtgc tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg
    27121 atgtgcatgc gttgaaagaa cagatgctgg aactgctaca gcagcgtggc gcgcaatacc
    27181 ctgccgagca taacgtcggt catttgtata aagcaccgga gacgttgcag aagttttatc
    27241 gcgagaacga tccgaccaac agtatgaatc cggggatcgg taaaaccagt aagcggaaaa
    27301 actggcagga agtggagtaa aaattacgga tggcatagta tcgccatccg tcattcactt
    27361 aatcgttctg tgccgtctgc cccgccgccg ccatttgggc ggctttttgt tttttatagc
    27421 tcaacgctgc tgctggaaca ggcatcactt taccggtttc aatccaggta cgcaggcggc
    27481 tggcatcggc aaaatgggta tatttgccaa acgcatccat cactaccagc gccaccggtt
    27541 tattattgat aaccgtacgc atcaccagac aatggcccgc cgcattggta aaaccggttt
    27601 tggttaactg aatattccag ttatcgcgat acaccagatg gttagtattg cggaacggca
    27661 gcgtatacgt cgggttagag aaggttgcca tatcttcccg ggtagtactt aactgcccga
    27721 tcaacggata ttgtttgctg gcaatgagca gtttggttaa gtcacgggca gttgaaacgt
    27781 tatgcaccga caatccggta ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg
    27841 ctttcgcatt cattgcctta ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc
    27901 ttgccgccgc gcggttttct gaagacatca gcgccagcaa cagcatatct ttacggctga
    27961 tttcgctatt cagtcgtacg cgcgaataga cccctttcat ctccggcgtc tggctgatat
    28021 ccacttttag tttttcatcc agcggcagtc gtgcatccag cacaaccatc gcggtcatta
    28081 atttgctgat agacgcaatc ggacgcacca gatccgggtg gttcgaatag atcactttgt
    28141 tggtattcag atcaacaatc atcgcgctac cggaggcaat ttccggttgt gaagcggtgg
    28201 tagcggctgc cgttttcgca acggcctgcg gtgcaaaagg cacagccagc atcagggcca
    28261 ggctaaataa agaaactcga aatttcggca tgatgagcat tcagatagtg gttcacgcgc
    28321 acgggttgcg caccgccgga gtaaggattt actgaggcta gcgacgccat cataacgagc
    28381 aaaaagtgcg atcgtcaaag gagaatcgtg aggaaatgct gcattgctga catttacgcc
    28441 agcaatgcaa cgtcaaagaa ctttctagaa caaacgataa ccgtagcccc atagtataac
    28501 ggtaagggcg agcagcactt ccagtaccag cacgccaatc gccagcgtcg aactggagaa
    28561 gctaaggcct tcctctttgt tgatattcag gaagctcgga ataccaaggt aaagcaggta
    28621 gccggtgtaa aacagcgcca ccgtgccgac cagcgcacac aaccagacca gcggataaag
    28681 cgccaccaga ccgcttaaaa acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg
    28741 cgcaagtgac ggacgctgcg gataattacg cgccatccac cagatgaccc gccccattac
    28801 cgcaacccca gccagcataa cgccataaaa caggacagcc agcgccagtc cggtaaacca
    28861 ggataacttc aggatagtgc catcgccaaa attccagcca atctgtgtag tgccaatgaa
    28921 ggcgcaaatc accggaatcg ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat
    28981 cgtttcgttt tcgcgattaa tcacctgcat ttcacgatcg ggatgggaaa acagtcccca
    29041 gacatggctc ataccgcccc cttgttgtga gttcatgaac ctgacagttc aagtataagc
    29101 cagcttgtga ttattttttg ttcatccatg taatttgcac cgtctgatgt atgtcctttt
    29161 tcagcgtgaa tgattcacag ggtgtatgct tacaggcaac caaagggaga cagactggcc
    29221 tatggatctc aatacactca tctcacaata tggttatgcc gcgctggtta tcggtagcct
    29281 ggcagagggt gaaaccgtga cgttgctggg aggcgttgcg gcacatcagg ggctattaaa
    29341 gttcccgctg gtggtacttt ctgtggcgct tggcggcatg attggcgacc aggtgcttta
    29401 tctgtgcggg cggcggtttg gcggcaagct attacgccgt ttctcgaaac atcaggataa
    29461 aattgagcgg gcgcagaaac ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg
    29521 ctttatgtat ggctttcggg tgattggccc gacgctgatt ggtgccagcc agctgccgcc
    29581 gaaaatcttt ctgccgctga atattctcgg cgcatttgcc tgggcgttga tttttaccac
    29641 tatcggttac gctggtggtc aggtgattgc gccgtggttg cacaatctcg accagcattt
    29701 gaagcactgg gtctggttga ttctggtcgt ggttctggtg gtaggcgtgc gctggtggct
    29761 gaaacgacgc gggaagaaaa agccggatca tcaggcgtaa aaccattgcc ctggataagg
    29821 cgttcgcgcc gcatccgaca tccggagtac catgcctgat gcgacgcttg tgcgtctttt
    29881 catgccgacc aaaatattac ccaatgaaat atccacgcac ctcactgcgc tattctgggt
    29941 tgaactgtgg attcgccaac ataaagccgc catccactat caacgactgc ccggtggtgt
    30001 aatttgcgcc ctccgaacaa agccacacca ccaggctggc aatctcatgc gttgcgccaa
    30061 aacgccgcaa gggaatcgaa ggctccgcgt cgggcttcac gtcgctgtca tccatgccat
    30121 tcattggcgt ggcgatcgcc ccaggcgcga ctgcgttcac caaaatctta tgcctgacca
    30181 gctccagcgc catcgctttg gttaacccac cgagcgcatg tttagcggct gtgtaagcgc
    30241 tggcatccgg cagcggcgta tgttcatgta ccgacgtaat gttgatgatg cgaccgccct
    30301 gcccttgttt caccatctgg cgggccgcaa tttgtgagca taaaaatgca ccatcgacat
    30361 caacggtaaa aatcttgcgc cactcatcga aagccatatc aagaaacggc gctttggtca
    30421 ttgcgcccgc attattcacc agcacatcaa tacgccccag ccgttgaatg agtttctcca
    30481 gcgcctgcgc accttctggc agtttaccga gatccagctg cacgatctcc gcacgtaagc
    30541 cgtggctaac tacctcacgc gcggtatctt ttgccccttc ttcatctgag tgccaggtaa
    30601 tgccaatatc aaacccctgc tgcgccagta ataacgcgca ctctttgccg atccccgaat
    30661 cggaggcggt aataatcgca acctgtgcca tcgagttctc cacttaacgc tgaataaacg
    30721 ttaagtatag aaggcgcata tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt
    30781 tgaatggaag catccagccg ctgcccttgc aataacaggc cattggcccg ctcacgcagc
    30841 gcggggattc tggcttcgct gacgcgggaa ccagcaatga tgcccgcgtt aaagcgcgcc
    30901 tgcgcaagac ctaccacacg caaggcatcg cgctcaattt gcgcctgatg ctggtttttc
    30961 tccgcaagtg tctgaacctg actggctgcc cgcgccacgt cattcaccgc ttcaaccacc
    31021 gctttgttgt agctggcgat agacaagttg ctttcggctt ttgcgatatc gagattggcg
    31081 ttaagacgac cactatcgaa aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc
    31141 gcggaatgac ggaacagatc gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg
    31201 ttgatgtcag gataaaatgc cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg
    31261 taccagtgcg ccgcctgcaa atctgcccgc cgggccagta aggagtaccc cagttcatca
    31321 ggaagctggc ttgccacttt cggcaacgcg accggtttaa gcttcaatga ctttgtctgg
    31381 ttatttgtaa gtgcgcttaa ccgtgcctca ataattttca ttttccccgc gacatcgttg
    31441 agctgctgcc gggttttgct ggcattaata tcggtttcca caccttcaac tgaagaagta
    31501 atcccgttct gatatagctg gcgatcggtc gcgataatgg tgttctgctc tttttctatt
    31561 tgctgcaaga ccgtgtttaa cgccgcctgg gtttgccact cccagtacag gcgggctacg
    31621 ctgccagcca gcaattggcg ggtttgctcg cgttccgccg cccgtgcttt aaccgtaccc
    31681 aggcgggcag taacctccgc ccgattcttt ccccagatat cgagatgcca gcccgccgtt
    31741 aagccaaaag ttccgttggt gtaccacgga ccggtcgtac ctgcggccgg atcgttcaga
    31801 gcaaacggcc ccattagccc ttctgccgac attttttgcc gctccatatc cgccgaaaag
    31861 tcgatctgcg gaccatcctg agtggcaact gccttcgcct gggcttcagc tagctgaatg
    31921 cgctgttcag ccacctgcat atccggtgcg ttctgtagtg cattgttaat taatgaagtg
    31981 agctgattat cgtgatactc cagccaccat tggctgtctg gccaaccatt tttcagcgcc
    32041 gtgggtaatg cggtgtcaac ttgtgcagcg ggcgtttgct ggcttaacgc ctggcgggtt
    32101 tcatgcatag gcgcacaccc ggccagcatc agtaacagcg gaaaacaggc gatggctgga
    32161 taaaaggaat cacgattcat gggggaataa tcaggtaaga aacggtgcgc ggagattacc
    32221 gtgtgttgcg atatattttt tagtttcgcg tggcaataca tcagtagcaa taaaacgaca
    32281 tacccagaaa aatatagact aagtaaatga tatcttccga tttatcttaa tcgtttatgg
    32341 ataacggcaa agggcttcgt tttttcctat acttattcag cactcacaaa taaaggaacg
    32401 ccaatgaaaa ttatactcta ggctgtattg attattttcc tgattgggct actggtggtg
    32461 actggcgtat ttaagatgat attttaaaat taattaatgt catcaggtcc gaaaataacg
    32521 agaatatttc agtctttcat cctgttgcgc tcctgtcatg tgcattgctt catata
//
LOCUS       DAJSKJ010000023        30961 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000023 DAJSKJ010000000
VERSION     DAJSKJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.023"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(22..1464)
                     /gene="pyk"
                     /locus_tag="ON345_000957"
     CDS             complement(22..1464)
                     /gene="pyk"
                     /locus_tag="ON345_000957"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR3071056.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(1592..2461)
                     /gene="hexR"
                     /locus_tag="ON345_000958"
                     /gene_synonym="yebK"
     CDS             complement(1592..2461)
                     /gene="hexR"
                     /locus_tag="ON345_000958"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR3071057.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVHHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            2799..4274
                     /gene="zwf"
                     /locus_tag="ON345_000959"
     CDS             2799..4274
                     /gene="zwf"
                     /locus_tag="ON345_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR3071058.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            4509..6320
                     /gene="edd"
                     /locus_tag="ON345_000960"
     CDS             4509..6320
                     /gene="edd"
                     /locus_tag="ON345_000960"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR3071059.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRNALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            6357..6998
                     /gene="kdgA"
                     /locus_tag="ON345_000961"
                     /gene_synonym="eda"
     CDS             6357..6998
                     /gene="kdgA"
                     /locus_tag="ON345_000961"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3071060.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(7053..8231)
                     /gene="purT"
                     /locus_tag="ON345_000962"
     CDS             complement(7053..8231)
                     /gene="purT"
                     /locus_tag="ON345_000962"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR3071061.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFRKAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSHRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAAQIKVQA
                     "
     gene            8365..8655
                     /gene="yebG"
                     /locus_tag="ON345_000963"
     CDS             8365..8655
                     /gene="yebG"
                     /locus_tag="ON345_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCR3071062.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8722..9078
                     /locus_tag="ON345_000964"
     CDS             8722..9078
                     /locus_tag="ON345_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR3071063.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            9405..10064
                     /locus_tag="ON345_000965"
     CDS             9405..10064
                     /locus_tag="ON345_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR3071064.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            10273..12333
                     /gene="ptrB"
                     /locus_tag="ON345_000966"
     CDS             10273..12333
                     /gene="ptrB"
                     /locus_tag="ON345_000966"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR3071065.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12330..12992)
                     /gene="exoX"
                     /locus_tag="ON345_000967"
     CDS             complement(12330..12992)
                     /gene="exoX"
                     /locus_tag="ON345_000967"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR3071066.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(13016..13672)
                     /locus_tag="ON345_000968"
                     /pseudo
     CDS             complement(13016..13672)
                     /locus_tag="ON345_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(13774..14004)
                     /gene="holE"
                     /locus_tag="ON345_000969"
     CDS             complement(13774..14004)
                     /gene="holE"
                     /locus_tag="ON345_000969"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR3071067.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            14143..14517
                     /gene="yobA"
                     /locus_tag="ON345_000970"
     CDS             14143..14517
                     /gene="yobA"
                     /locus_tag="ON345_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR3071068.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            14521..15393
                     /gene="copD"
                     /locus_tag="ON345_000971"
     CDS             14521..15393
                     /gene="copD"
                     /locus_tag="ON345_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR3071069.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            15406..15747
                     /locus_tag="ON345_000972"
     CDS             15406..15747
                     /locus_tag="ON345_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR3071070.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQSISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            16142..16798
                     /gene="pphA"
                     /locus_tag="ON345_000973"
     CDS             16142..16798
                     /gene="pphA"
                     /locus_tag="ON345_000973"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR3071071.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQYLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(16799..16990)
                     /locus_tag="ON345_000974"
     CDS             complement(16799..16990)
                     /locus_tag="ON345_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR3071072.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(17095..17331)
                     /locus_tag="ON345_000975"
     CDS             complement(17095..17331)
                     /locus_tag="ON345_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR3071073.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(17449..18888)
                     /gene="rsmF"
                     /locus_tag="ON345_000976"
     CDS             complement(17449..18888)
                     /gene="rsmF"
                     /locus_tag="ON345_000976"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR3071074.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTFSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEVLIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(18969..21602)
                     /gene="yebT"
                     /locus_tag="ON345_000977"
     CDS             complement(18969..21602)
                     /gene="yebT"
                     /locus_tag="ON345_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR3071075.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQVPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(21571..22854)
                     /gene="yebS"
                     /locus_tag="ON345_000978"
     CDS             complement(21571..22854)
                     /gene="yebS"
                     /locus_tag="ON345_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR3071076.1"
                     /translation="MALNTPQITPTKKIKVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRLVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            22984..23481
                     /gene="msrC"
                     /locus_tag="ON345_000979"
     CDS             22984..23481
                     /gene="msrC"
                     /locus_tag="ON345_000979"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR3071077.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            23578..24276
                     /gene="proQ"
                     /locus_tag="ON345_000980"
     CDS             23578..24276
                     /gene="proQ"
                     /locus_tag="ON345_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR3071078.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            24296..26344
                     /gene="prc"
                     /locus_tag="ON345_000981"
     CDS             24296..26344
                     /gene="prc"
                     /locus_tag="ON345_000981"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR3071079.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFVKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            26536..27417
                     /gene="htpX"
                     /locus_tag="ON345_000982"
     CDS             26536..27417
                     /gene="htpX"
                     /locus_tag="ON345_000982"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR3071080.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(27463..28836)
                     /locus_tag="ON345_000983"
     CDS             complement(27463..28836)
                     /locus_tag="ON345_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3071081.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            29013..29804
                     /gene="kdgR"
                     /locus_tag="ON345_000984"
     CDS             29013..29804
                     /gene="kdgR"
                     /locus_tag="ON345_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR3071082.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(29947..30186)
                     /locus_tag="ON345_000985"
     CDS             complement(29947..30186)
                     /locus_tag="ON345_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071083.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            30345..30488
                     /gene="mgrB"
                     /locus_tag="ON345_000986"
     CDS             30345..30488
                     /gene="mgrB"
                     /locus_tag="ON345_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR3071084.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            30563..30850
                     /locus_tag="ON345_000987"
     CDS             30563..30850
                     /locus_tag="ON345_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR3071085.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         7903 a         7626 c         7963 g         7469 t
ORIGIN      
        1 tcgccgcatc cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc
       61 cacggtactc atcacgtcgc cctgggtgac aatcaccagg tcaccagaca tcaagtaacc
      121 tttatcgcgc agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc
      181 aaagtgcact ggcgtaacgc cgcgatagag agcagtcagg ttcagcgtac gttcatggcg
      241 tgacatggcg aaaattggca gaccagagct gatacgggag gtcatcagcg ccgtacgacc
      301 cgattcggtc atggtgatga tcgccgtaac gcctttcagg tggttagccg cgtacattgc
      361 tgacatggca atagcttctt ccacattgtc gaactgcacg tccagacggt gtttagaaac
      421 gttgatgctc gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc
      481 tgaaggatac tgaccagcag cagtttctgc agaaagcatc acagcgtcag taccatccag
      541 aacggcgttt gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga
      601 ctccatcatc tgggtcgccg tgattactgc gcggttaagc tgacgcgcac gacggatcaa
      661 cgctttctga atgccgacca gttccggatc gccaatttcc acaccaaggt cgccgcgtgc
      721 aaccattacc acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcatacggc
      781 ttccgcacgt tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccagccg
      841 acgggcatag ttcagatctt cgccgcagcg tgggaaggag acagccaggt aatctacgcc
      901 aattaacgcc gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa
      961 accgccgcca agtttgttga tacctttatt gttggagagg ggaccaccga cggtcacttc
     1021 ggtgaacact ttcatgccct gaacttccag tacttttaac tgaacgcgac catcgtccag
     1081 cagcaggatg tcaccaggca cgacgtcagc aggcaggcct ttgtagtcga taccgacttt
     1141 ttctttgtcg ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt
     1201 gaggaaaact ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc
     1261 cagaatagcc acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat
     1321 tttgtgatct tcaggcgagc cgtgagaaaa gttcatacgt acaacgtttg cacccgctgc
     1381 gataactttt tcaaggttat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt
     1441 agttctgcga agccttctgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct
     1501 tgaacatgaa atccgaatga aataacgccg cgatggcgat aaattaatct cattcggatg
     1561 tcgtataaga ttaggacagt gacagtcgtt tttagcgatc gtcacttaaa ttaagtaact
     1621 gcttatcaaa acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt
     1681 ttgcaccgcg tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgcgcaa
     1741 gtcgagaaac catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca
     1801 gcgttgcttc ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atggcgtcgt
     1861 tttcgcgtgc cagctgcgcc agctcgacca gattttttgt tcttcccgtg tgagaaatca
     1921 gcaccaccac gtctccgtcg ctacaattca tacaactcat gcgttgcagc acaatatcat
     1981 cggagtacac caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg
     2041 cggctgaaga gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg
     2101 cgcggttgat ggcagattta tccagtgaat gatggacatg atcgagcgtt gccattgcgg
     2161 actcaaatat tttccccgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa
     2221 catagggagt gccattcgcc agactttgtg ccagatgaag tttaaaatca ggaaaaccgc
     2281 gcgtatccat gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagcg
     2341 ccagagcagc aatactcgaa tggatcgcgt tatcgggaga agccagaatg acctcggcaa
     2401 ctttgcgctc tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca
     2461 tgatgtaaag agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt
     2521 tttgtcagat attacgcctg tgtgctgtgt taatgacaaa agcagataaa aaagttgtta
     2581 ttttttttca taacatgatc agtgtcagat ttttacccaa tggaaaacga tgattttttt
     2641 atcagttttg ccgcactttg cgcgctttta ccgtaatcgc acgggtggat aagcgtttac
     2701 agttttcgca agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct
     2761 ggcttaagta ccgggatagt taacttaagg agaatgacat ggcggtaacg caaacagccc
     2821 aggcctgtga cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc
     2881 cttccctgta tcaactggaa aaagccggtc agctcaaccc ggacacccgg attatcggcg
     2941 tagggcgtgc tgactgggat aaagcggctt ataccaaagt tgtccgcgag gcgctcgaaa
     3001 ctttcatgaa agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt
     3061 tttgtaatct cgatgtcaat gacactgctg cattcagccg tctcggcgcg atgctggatc
     3121 aaaaaaatcg tatcaccatt aactactttg ccatgccgcc cagcactttt ggcgcaattt
     3181 gcaaagggct tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac
     3241 cgctggggac gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg
     3301 aggagtgcca ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt
     3361 tggcgctgcg ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc
     3421 atgttgagat taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata
     3481 aagccggtca gatgcgcgac atgatccaga accacctgct gcaaattctt tgcatgattg
     3541 cgatgtctcc gccgtctgac ctgagtgcag acagcatccg cgatgaaaaa gtgaaagtac
     3601 tgaagtctct gcgccgcatt gaccgctcca acgtgcgcga aaaaaccgta cgcgggcaat
     3661 atactgcggg cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga
     3721 acaagagcag caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct
     3781 gggccggtgt gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag
     3841 tcgtggtcta tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc
     3901 cgcagaataa actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga
     3961 ataaagttcc tggccttgac cataaacata acctgcaaat caccaagctg gatctgagct
     4021 attcagaaac ctttaatcaa acgcatctgg cagatgccta tgaacgtctg ctgctggaaa
     4081 ccatgcgtgg tattcaggca ctgttcgtac gtcgcgatga agtggaagaa gcctggaaat
     4141 gggtagactc cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg
     4201 ccggaacctg gggacctgtt gcctcagtgg cgatgattac ccgtgatggt cgttcctgga
     4261 atgagtttga gtaatatctg cgctcatcct ttatggttat tttaccggta acatgatctt
     4321 gcacagattg tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct
     4381 ttttttatta cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcag
     4441 tcataaatcc tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag
     4501 gagcctttat gaacccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg
     4561 agactcgctc tgcttatctc gcccggatag aacaagcgaa aacttcgacc gttcatcgtt
     4621 cgcagttggc atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaagacaaag
     4681 cctctttgaa aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc
     4741 tctccgcgca ccagccttat gaacactatc cagaaatcat tcgtaatgcc ctgcatgaag
     4801 caaatgcggt tggtcaggtt gcaggcggtg ttccggcgat gtgtgatggt gtcacccagg
     4861 ggcaggatgg aatggaattg tcgctgctaa gccgcgaagt gattgcgatg tctgcggcgg
     4921 tggggctgtc ccataacatg tttgatggtg cgctgttcct cggtgtgtgc gacaagattg
     4981 tcccgggtct gacgatggca gccctgtcgt ttgggcattt gcctgcggtg tttgtgccgt
     5041 ctggaccgat ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg
     5101 ccgaaggtaa agtggaccgc atggcattac tggagtcaga agccgcgtct tatcatgcgc
     5161 cgggaacatg tactttctac ggtactgcca acaccaacca gatggtggtg gagtttatgg
     5221 ggatgcagtt gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga
     5281 ccgcagccgc tgcgcgccag gttacacgca tgaccggtaa tggtaatgaa tggatgccga
     5341 tcggtaagat gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg
     5401 gtggttccac taaccacacc atgcacctgg tggcgatggc tcgcgcggcc ggtattcaga
     5461 ttaactggga tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc
     5521 cgaacggtcc ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc
     5581 gtgaactgct caaagcaggc ctgctgcatg aagatgtcaa tacggtggct ggttttggtc
     5641 tgtctcgtta tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg
     5701 cggaaaaatc actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg
     5761 gtgggacaaa agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc
     5821 cggttgagaa ccaggtgatt gaagcgcctg cggttgtttt tgaaagccag catgacgtta
     5881 tgccggcctt tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg
     5941 ggccaaaagc gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat
     6001 tggaccggtg tttcaaaatt gcgttagtta ctgatggacg actctccggc gcttcaggta
     6061 aagtgccgtc agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag
     6121 tgcgcgacgg ggacatcatt cgtgtgaatg gacagacagg cgaactgacg ctgctggtag
     6181 acgaagcgga actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg
     6241 gaacaggacg tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg
     6301 caacctgtat cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga
     6361 aaaactggaa aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg
     6421 tggtaaaaaa actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc
     6481 gcgttctgga agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca
     6541 aagaagtgcc tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag
     6601 aagtcaccga agcgggtgca cagttcgcaa ttagcccggg gctgaccgag ccgctgctga
     6661 aggctgctac cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga
     6721 tgctgggtat ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg
     6781 gtgtgaaagc cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg
     6841 gtggtatttc tccggctaac taccgtgact acctggcgct gaaaagcgtg ctgtgcatcg
     6901 gtggttcctg gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta
     6961 agctggcgcg tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatcgagg
     7021 atcgggcatt tttgtagcgt ttttcgccgg ggttaagctt gtacttttat ctgtgctgcg
     7081 gcgtgctttg cgcgctcaat cgcttcatca acactctctg cagtagccag tgccaccccc
     7141 agacgacggc tgccatcaat ttccggctta ccaaataaac gaatctgtag gcctgcgcct
     7201 acggcattct gcacattatc aaacgtgaca ttctgactgg tcagttgtgg caggataacg
     7261 gcagaagctg caggaccata ctgacggatc ccgccaaccg gaagtccgag gaaggcacgt
     7321 acatgcaggg caaactccga gagatcctga gaaattaacg tcaccatccc ggtatcatgt
     7381 gggcgatggg agacctcact gaaaatcacc tcatcaccac agacaaacag ctcgacacca
     7441 aacagcccat aaccgcccag tgccagcacc actttacggg caatttcttg cgcacgttcc
     7501 aaagccagct ggctcatttg ctgtggttgc caggattcac gatagtcgcc atcttcctgg
     7561 cgatgaccta ccggtgcaca gaaatggacg ccatccaccg cgctgacggt tagcagggta
     7621 atttcgaaat caaacttaac gacgccttca acaattacgc ggcccgctcc ggcgcgaccg
     7681 ccttgctgag cgtactccca tgcatgagca agttgctctg cagaacgaat aaacgtctgc
     7741 cccttgccgg aggaactcat caccggttta acaatgcagg gatagccgat ttcagcaacc
     7801 gccttgcgga aaaggttttc gttatcggca aaacgataag tggaagtggg cagctgcagc
     7861 tcttctgccg ccaggcgacg gataccctcg cgattcatcg ttaatttcgt tgcgcgcgcg
     7921 caggggacaa cattcagtcc ttcctcttca agttggatca gcatatcggt ggcaatagct
     7981 tcgatctccg gcacgatata atgtggtttt tccagttcaa ccacacggcg taatgcatca
     8041 ccatcaagca tattaatgac atgggagcga tgcgcgacat gcatagctgg cgcgtcggca
     8101 tagcgatcga cggcaatcac ctcaacgccg agacgctgac actcgattgc cacttcttta
     8161 cccagttcac cggagcctaa taacatcacg cgagttgctg ccggacgcag cgcagtgcct
     8221 aataacgtca taacgaatat cccctgatta attctgcgcg cagtataaac gaaaacgttt
     8281 gcgcgtgtct ttattgctga tgttgatttc ccaaccgaaa agaaatatac tgtataaaat
     8341 cacagttatt atgagaggct gcaaatggcg gttgaagtta aatacgtagt cattcgtgag
     8401 ggcgaagaga aaatgtcgtt taccagcaaa aaggaagccg atgcttatga caaaatgctt
     8461 gatacggcgg atctgcttga cacctggctg acaaattctc cagtgcaaat ggaagacgag
     8521 caacgtgaag ccctttcgct atggctggca gaacaaaaag atgtgctgag caccattctg
     8581 aaaaccggca aattaccgtc cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc
     8641 tcacacgcgg cctgaagcaa cttgcgctcc tgacaatttg taccatgctt tctcctgaat
     8701 cattgtgcta tggagaaaaa catgaaaaaa agaggggcgt ttttagggct gttgttggtt
     8761 tctgcctgcg catcagtttt cgctgccaat aatgaaacca gcaagtcggt cactttccca
     8821 aagtgtgaag gtctggatgc tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa
     8881 aatcgcgtgg cgcgttgggc agatgatcaa aaaattgtcg gtcaggccga tcccgtggct
     8941 tgggtcagtt tgcaggacat tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg
     9001 cgtggtaaaa gtgccgatat tcattaccag gtcagcgtgg actgcaaagc gggaatggcg
     9061 gaatatcagc ggcgttaatg gcttctgata attcgattga ccgttggcgg atgcggcgcg
     9121 agtgccttgt ccgccccaga acgcaccaca atcgtaggcc tgagaagacg caacagcgtc
     9181 gcatcgggca atcagcatca aacaatgcca ctcaaaacga caaactactt tcacctaaaa
     9241 tacccttatc acctgatata cagtaaatac cttacctggc gcgaatttct ttgaataacg
     9301 gcctaccgcc ccgcttattt gcgcatcttt tgtctcgata tacattttct gacatcctca
     9361 tccgctatgc tacaggcact aaaaatggtt attgaggagc aaatatggct aactggttaa
     9421 atcaactgca atccctgctt gggcaaagca gttcttctac ctcctcgtct gcggatcagg
     9481 gattgggcaa actgttagtg ccaggcgcat taggcggact ggctgggctg ctggtcgcaa
     9541 ataaatcagc acgtaaactt cttacgaaat atggcaccaa cgcgttactg gttggcggcg
     9601 gagcggtagc gggtacggtg ctgtggaata aatacaaaga taaaattcgc gcggcgcatc
     9661 aggacgaacc gcagtttggc gcgcaaagta cgccgctgga tgagcgtaca gaacgtttga
     9721 tccttgcgct ggtctttgcc gctaaaagtg atggtcatat tgatgccaaa gaacgtgcgg
     9781 caatcgacca gcaattgcgt gaagccggcg tggaagagca ggggcgtgta ctcattgagc
     9841 aggcaatcga acaaccgctg gatccacaac gcctggctac cggggtccgc aatgaagaag
     9901 aggcgctgga aatctatttc ctgagttgcg cggctattga tattgaccat tttatggaac
     9961 gcagttacct gaatgcactg ggtgacgcgc tgaaaatccc ccaggatgtt cgtgacggca
    10021 ttgaacgcga tctcgaacag caaaaacgca ctttggcgga ataattccgc tctctggata
    10081 gcctgaagtt gactatttcg ggctatccag aatcgcacag ccatttgaac aggctgattt
    10141 tctctgtctg cataacattc gatatccgcg ccattacctg tcatcatcta agcaatgact
    10201 cccctgtttc gcttgcatcc ccggtgagtt ttgccaccct tataagatgt ttcaaccaga
    10261 aagaacaata acatgctacc aaaagccgcc cgcattcccc acgccatgac gcttcatggc
    10321 gatacgcgca tcgataatta ctactggctg cgggacgata cgcgttctca gccagaagtc
    10381 ctggactacc tgcaacaaga aaatagttac ggtcatcggg tgatggcctc acaacaagcc
    10441 ttgcaggatc gcatcttaaa ggaaatcatc gaccgcattc cgcaacgaga agtttctgcg
    10501 ccctacatca aaaatggcta ccgctatcgg catatttatg aaccaggctg tgaatatgct
    10561 atctaccagc gtcaatcggc attcagtgaa gagtgggatg agtgggaaac attgctcgat
    10621 gccaataagc gcgcagctca tagtgagttt tattcgatgg gcggaatggc gattacgccc
    10681 gataacacca ttatggcgct ggcagaagat tttctttccc gacgccagta cggcattcgt
    10741 tttcgtaatc tggaaactgg taactggtac ccggaactgc tggataacgt tgaacccagc
    10801 tttgtctggg caaatgactc ctggactttc tactatgttc gcaagcatcc ggtgacgctg
    10861 ctgccttatc aggtctggcg tcacgccatc ggtacgccag catcgcaaga taaactgatc
    10921 tacgaagaaa aagacgatac ctattacgtc agcctgcata aaacgacctc gaagcactat
    10981 gtagtcattc atttggccag cgccaccacc agtgaagttc gcctgctgga cgcggaaatg
    11041 gccgatgccg agccgtttgt ttttctgccg cgccgcaaag atcacgaata cagccttgat
    11101 cactaccagc atcgttttta tctgcgttcc aaccgccacg gcaaaaactt tggcttatac
    11161 cgtacccgta tgcgtgatga gcaacagtgg gaagagttaa ttccgccacg cgaaaacatc
    11221 atgctggaag ggtttacgct gtttaccgac tggctggtgg ttgaagagcg tcagcgcggg
    11281 ttaaccagtt tgcgccaaat taaccgcaag acccgggaag tcattggtat tgcctttgat
    11341 gatccggcct atgtgacctg gattgcctac aatccagaac ctgaaaccgc gcgattgcgt
    11401 tatggttatt cttccatgac cacaccagac actttgtttg aactggatat ggataccggt
    11461 gagcgtcgtg tattaaaaca aacggaagtt cctggttttt atgcggcgaa ttaccgcagt
    11521 gaacacctgt ggatagtcgc ccgtgatggc gtcgaagttc cggtttcgtt ggtctaccat
    11581 cgcaaacatt ttcgcaaagg acacaacccg ttgctggtgt atggctatgg ttcttacggc
    11641 gcaagtattg atgccgattt cagttttagc cgcttgagtt tgttagatcg tggctttgtc
    11701 tacgccattg tccatgttcg cggcggtggt gagctggggc aacaatggta cgaagacgga
    11761 aaatttctga agaagaaaaa tacgtttaat gattatcttg atgcctgcga tgcattgtta
    11821 aaactgggct atggctctcc ttcgctttgt tatgcgatgg gcgggagtgc ggggggcatg
    11881 ttgatgggcg ttgcaattaa tcaacgcccg gaattattcc acggcgttat cgcccaggta
    11941 ccgtttgttg atgttgtaac aacgatgctt gatgaatcaa ttcctcttac cactggtgag
    12001 tttgaagagt ggggtaaccc gcaggatccg caatattacg agtacatgaa aagctacagc
    12061 ccgtatgaca acgtcaccgc acaggcttat ccgcatttac tggtaacgac cggtttgcac
    12121 gattctcagg tgcaatattg ggaaccggca aaatgggtcg ctaaattgcg cgagctgaaa
    12181 accgatgacc atcttttatt gctctgtacc gacatggact caggccatgg cggtaaatct
    12241 ggtcgcttta aatcgtacga aggcgtagcg atggaatatg cttttctggt cgcgctggcg
    12301 cagggaacat tacccgctac gcctgcggac taagtatttt ccagataatg tttcagtgtt
    12361 aaacgcagct ccgggctcat gctgtccagg ttattaaata accagcgcag atagcccgga
    12421 tcgcgttcgg caacgtcgga aaccgctttg ccacggtatt tgccaaaggt gaaggtcgtc
    12481 atcaacgacg gacgtccggt gatatcggcc atctgttctg ccgtccagcc ggaggtgttc
    12541 ataatatcga taagcaacgc ggcggtgata taacagtcat acaacgcgcg gtgatgatgc
    12601 aggcccggcg gcgtctgtac attgagcttg cgtgttttat ataacgccat attgctgtac
    12661 ttgatccctg gccacaatcg acgggccagt ttcatagtgc aaatccactc accgggcatc
    12721 tcaggcagta cgcggcggtc aaagctggcg ttatgcgcga cataccattc actaccgtaa
    12781 tagtgtggga tcacatcttc aatccacggt ttatcggcga ccatggcttc ggtgatgcga
    12841 tgaatcgcca tcgcttgcgg actaatagga cgatcgggac gcaccagatg gctcatgggg
    12901 ttgacgattt ttccgtcaat gacatcaaca gaggcaatct caacgatccc tccctgcaaa
    12961 ccgcaggttt ctgtatcgat aatgcgcaac atggcatgct ccaggccgaa aaagcctagc
    13021 gtaatggaat gatatcgcct tgccaaccct gtgatgaacg ctgaccgact aacaataatg
    13081 aaccacgatc ggcgcgaacg atgagacgac catattcatc ccatagtgca ctgttaccac
    13141 gggcatttgc catcagcacg gcaatcgagt actgatgtga aaaaaattgt aagcgacggg
    13201 ccgacgccag caggtcgggt tctcccagac attgccctgt agtaaacagt gaacaggtcg
    13261 ggtccatatc cataccttgc ggttgttcat cgaccaccgt gatggttctg gaacgacgac
    13321 ccagacatgc accatgactc tgatggtaga tcccaggcgt ttttcgccac ggtgcgaaca
    13381 ccgcaattcc acgaataaag cgatcgttat attcaacggg aaggccggca atgatggtca
    13441 ttcgctaggt cgttgcggca taacaaagcg ggtctaacag tgagagatcg ggtggggcag
    13501 gaagggcacg tcgcgaataa tcacacccca gtaaagaaag cgatggaaaa actaacagct
    13561 gacattgttg cttggcggcg gccctcacaa actccagatg atgagctacc tgttcggtga
    13621 gtgaggtttt acgaggctca tattgcgctg ctgcaacttt ccagaacgac ataatgactt
    13681 ccttttcaaa atgactgcaa attatgggtg cggtttagaa aatactcata atttagcagg
    13741 agttatgtaa gaaagtgtaa ctctgtataa gttttattta agtttgggct cgtaaggtaa
    13801 acgtgacaga ttgaccgaag ccaaacggtg ggcaataagc cgctcgcgaa accagctgcg
    13861 caaatgttca ggctgttcac gttcaaccgc ttcagcgatc accggcatat tgtagcgttc
    13921 tttaaatgcc accccggccg ccgccaaatc gacattcact ttatccattt ctgtttgatc
    13981 cagtttagcc agattcttca gcattcttaa tctcctcatc attcgcgggc aaaagttact
    14041 aagagtgagc caggatggca agccggaatt gatgatcctc gactcagaca ttagacaagg
    14101 ttatgctctg taaaaaagtt attacaaaaa ggaatagata atatggcttc aactgcacgc
    14161 tcccttcgct acgcgctggc aatcctgacc acttcgttgg tgacaccttc cgtctgggca
    14221 catgcgcatt taacgcatca gtatcctgcg gcaaacgcgc aagtgacagc tgcaccgcag
    14281 gcaatcactt taaacttctc ggaaggtgtt gaaacaggat tcagtggtgc aaaaatcacg
    14341 gggccaaaaa acgaaaatat taaaacatta ccggcgaagc gaaatgagca ggaccaaaag
    14401 caactgattg ttccactggc cgattcgctg aaacccggaa cctacaccgt tgactggcat
    14461 gttgtttcgg tggacgggca taaaacgaaa ggacactaca cctttagcgt gaaataaatc
    14521 atgctggcgt ttacctggat cgcgctgcga tttatccatt tcacctcgct gatgctggtt
    14581 tttggcttcg caatgtacgg tgcctggctg gcccccttga cgattcgtcg cttgctggcg
    14641 aagcgttttc tgcgtttaca acagcacgct gccgtctgga gtttgatcag tgccactgca
    14701 atgctcgccg ttcagggcgg gttgatggga acgggatgga cagatgtatt ttcgccaaac
    14761 atttggcagg ctgttttgca aacgcagttt ggtggcgtct ggttatggca aattgttctc
    14821 gccctcgtaa cgttgatcgt cgcccttatg caaccgcgga atatgccacg tttgctgttt
    14881 atgctcacta ccgcgcaatt tattctgctg gcgggagtgg ggcatgcgac gctgaatgaa
    14941 ggggtaacgg cgaaaatcca tcagactaat cacgcgattc acctgatttg tgcggcggcg
    15001 tggtttgggg gattactgcc ggtgctctgg tgtatgcaac tcatcaaagg ccgttggcga
    15061 caccaggcta ttcaggcgct gatgcgtttt tcatggtgcg ggcattttgc ggtgatcggc
    15121 gtactggcaa gcggcgtgct taatgcattg ttgattaccg gatttccccc cacgcttacg
    15181 acttactggg gccaactctt attgctcaaa gccatcctgg tgatgattat ggtggtcatt
    15241 gcgctggcta atcgttatgt tcttgtaccg cgcatgaggc aggatgaaga tcgtgcggca
    15301 ccctggtttg tgtggatgac taaactcgaa tgggcgatag gtgcggtagt gctggtgatc
    15361 atcagcctgc ttgcgactct cgaacctttt tgatggatta cgaaaatgat gaaaaaaagt
    15421 attctggcat ttctgttact caccagttct gcagcggcgc tggctgcacc gcaggtgatt
    15481 accgtcagcc gttttgaagt gggtaaagac aaatgggcgt ttaatcgcga agaggtgatg
    15541 ctgacttgcc gaccgggtaa tgctttgtat gttattaatc cgagtaccct cgtgcagtat
    15601 cctttaaacg atatcgcaca aaaggaagtt gccagtggga agaccaatgc ccaatccatt
    15661 tcggtgattc agattgatga tcctaacaat cccggcgaaa aaatgagtct ggcaccgttt
    15721 atagaacgag ctgaaaaact ctgttaatta cctaaaatag ccttttgatt tccaataaaa
    15781 aaccgcctca gttctttcac cagaacgggc ggtttttaac atttaagctg atgaccaccg
    15841 cgctttttat tgaccatttt gcacgcaaac tggaaaacct ggcgtcgtca tctattctta
    15901 aagggcaagg caactaagcc tgcattaatg ccaactttta gcgcacggct ctctcccaag
    15961 agccatttcc ctggaccgaa tacaggaatc gtgttcggtc tctttttatc tgttaaaagc
    16021 cagaaacatt cccttcgctg actttatagt caaccataac acacactcta ctgtctgagt
    16081 ccagtgtttt ttaacatttt tgttaagatt atgtgatctt tagcgcggga ggaaaatatt
    16141 gatgaaacag cctgcgcccg tttatcagag aattgcgggt catcaatggc gacatatctg
    16201 gctttctggc gatatacacg gttgtcttga gcagttgcgc cgcaaattat ggcattgtgg
    16261 ttttgatccg tggcgagatt tacttatctc agtgggagac gttatcgatc gtgggccgca
    16321 aagtttacgt tgcctgcagt tactggaaca acattgggtt cgtgcggtaa gaggcaatca
    16381 tgaacagatg gcgatggatg cgctggcatc ccagcagatg tctttgtggt tgatgaatgg
    16441 cggcgactgg tttattgcgc tggcagataa tcaacagaaa caagcgaaaa cggcgctgga
    16501 aaaatgtcag tatttgccct ttattcttga agtacacagt cgcaccggca aacatgttat
    16561 tgctcatgcc gattatccag atgatgttta tgaatggcaa aaggacgttg atttgcatca
    16621 ggtcttgtgg agccgctcgc gattaggtga acgccaaaaa gggcagggaa ttacaggtgc
    16681 tgatcatttc tggtttggtc atacaccgtt gcgccatcgc gtggatattg gcaacttgca
    16741 ttatattgat accggcgctg tctttggggg cgaactgact cttgtgcaat tgcaataatt
    16801 aaaaatcacc gtactcctgt gcaggtcgcc agaaaccatc tataaaatcc tcaatcggaa
    16861 aacaaccgcc atggcggatc cgttgatcgc tcatagaata aagacactgc tgttccgtgt
    16921 tgtagacatc cacaacaata tcttcacaac cgccatccag gtagcaaaca aaaagtacca
    16981 gcgcgaacat ttcatccccg aagtgtggtg ccgtaccgtt aagtttagga gagattttac
    17041 aacgggggaa taaccaggac aaataacccg ccatgccgac gggttctttt tggatcaggc
    17101 aagacgcata atccaggcat cagattgttg atcgtaacgg gtacggtaaa ggacagagtg
    17161 ttcgccattt agcagggcag ggatactggt ttcagcatcc caggcatcca gttgcataca
    17221 taaatcgggg tcagatttac aaggaatggt taacgttcga tcacctggcg attcaccgtg
    17281 taattcgccg tcgtcgattt caaaagcgcc tatgcgcaca ctggttttca tcttcgtgct
    17341 ctccatttag ctgccgattg tggtacgacc agtccgatcg caccattttt cagcctagtt
    17401 gaggcagccg aatgtgccag tcaaaaagtg cgcttttttt gtgcgctgtc aggcgttacc
    17461 ggtaaaaagt ttcccatcgc gcaccagttc acgcggatag ctgtttttca atcgcgaacc
    17521 aatccgtttg gctaaaccaa tcggttgatg ctggaaagta accaatacat catccgccac
    17581 tggcgcggct tgcgggtaaa catcgcgccc gcgataccac tcctccgctt cctgcggtgt
    17641 cagctcgaat gcattcatat tatcgggtga ggcaagagca ataaccgctt catgctgcca
    17701 gcgataacct ttgttgtgcg tctcggcaag tttaatcccc aaccgagaaa atcggacttt
    17761 accgatcagg acttcaatgc ccaccgggaa caaccacagt tctttgtcac gctgccagag
    17821 gcgcaggttt tcatcccagt ttaagccaac acctgcagcc gcctgacgaa tttgtccagc
    17881 ttcgcgatct ttcaccgggc tgaacggaaa attaccgact ttgtatttgg gggcgggtaa
    17941 ggcgggaatc gcctgagttt tacgcagacg agcaacgaag aagccttcgc agtcgtaaat
    18001 ttgtgggaaa acatgcaaaa agccttcctc ggtcagcgcc ttgtttgcac cagggaagag
    18061 atcaccaagc ggtaaaaact ctactgcgtc ggggtaagtc tctttcagcc acaggcaaac
    18121 ggcttcgttt tcttcctggt ttaaggtaca ggtcgagtaa accagcgtac cgccagggcg
    18181 taatgcatga aaggcgctgt cgatcagttc ccgttgggta gctgcgattt cctgattgct
    18241 ttctggtgac cagtttttta gcgcatcggg atctttacgc accacgcctt cgccggagca
    18301 gggagcgtcc agcaaaatgg catcaaacat ttctggcact gccgcaccaa acacgcggcc
    18361 atcaaaatgt gtgagcgcaa cattactgat accacagcgg ctgatattgg catgtaacac
    18421 ttttacccga ctggcggaaa actcattggc aagaattgcc ccttcgttat tcatccgcgc
    18481 ggaaatttgc gtcgttttgg agccaggcgc ggcagcgaca tccatcaccc gctgtggtgc
    18541 attaccgtca gcaaacaagg cggcgacggg caacattgaa ctggcttcct gaatataaaa
    18601 caggccactt aaatgctcgg cggtactacc caatggcaat gcatcttcat tgtcgcgttc
    18661 aatccagaaa ccttcttcac accacggaat tggcgtaagc gtccagccat aaggagcggt
    18721 taattgcagg aaatcagcaa cggagatttt cagcgtatta acgcgaatgc tgcggcgcaa
    18781 cgggcgctga caggcggcaa gaaaatcatc aaaagagaac gtcgaaggca tcgcttcgcg
    18841 catttgcgtc agaaaggcgt ccgggaaata aacggtgtgt tgggccacga gcatatacca
    18901 ctggcaaaaa aaacaggcgc gcagtttagc ataaacgctc cggcgcaggc acgccggagc
    18961 agtgggcatt atttgggaag cgcagtaccc cattcacgcc actctttcgg ttcactttcc
    19021 tgcaacagga agtgcttgcc ttcctgggct ttcggtgcca gtggcgtacc cggaggcgtg
    19081 gcgaaggcga tgccgccacg gataaactgg ttaaaggtgc cggttttcac tacgccgccc
    19141 gtcagaccaa agtccagact gtaacccgat gccaaccaga agacggaatt gttacgcacc
    19201 aggtgttgat agcgtttact gatgcgcatc gcaatcatca cgcgatctga caatgtcccc
    19261 agcgtcattc cggtaaccgt acctacttcc agaccacgga acagcacagg cgtaccaata
    19321 cctaacgaac cggcttccgg tgcttcgaca ataatgctta agccatccag gtaacgcgaa
    19381 tcagtaatag tagcttcttg caactcaaag tcacggcgag gattaccccg acctggttcg
    19441 acattgatat acggctggag gatagtatct agatgctcaa cgcccgcggc tgaaatttgc
    19501 ggtgtgacca ccgagaagcg cgtaccgccg cgggcgaagg tctggacata ctccggatag
    19561 agcaccgcct ttgcctgtac ttcattgcgc gcggtaatca gatccagcgt ctggatttgc
    19621 ccgatatcaa taccgagata gcgaattggc atcccgaccg ccagttttcc ggcatcgaaa
    19681 gcgtgaagcg taatctgccc gccaaccgca cgggccgctg tttcggaagc atacagaata
    19741 cgcttatcgc ctttacgctg actggcgctg gcaccgctga ggttatcgaa gctaatggct
    19801 ccctttaatg ctctggagag cggggatgcc tgtacggtca ggccactacc attcagctga
    19861 actttcgccc cgccttctgc ccagaacacg ctattgctgg tcagaaggtt gcgatactcc
    19921 ggcttaatat gcagatcgat atcaaacgcg ttagctcgcg gacggacggt aataacttca
    19981 ccaacttcaa atttacggta gagcactacc gatcctgcct gcacatccgg cagcgtctct
    20041 gcactcaaac tcacggttgt ggtgggtaaa tcgctaaggc tgttctccag cgctttttcc
    20101 agattggcat acagtggata gctggctttc atctcgcctt tatcgcccgg cagaatacgt
    20161 atcccgccgt taatccattc tgaggcgctg gcaccgagaa actcaacgcc atccagcccc
    20221 accttcacgt cgacacggct gttgacaaca aatttgctat cgccttttac cagttcgcga
    20281 tgctgaggct cgatggcgac ggtaaaggtg acgcctttgc tggtgagttt acgatcgata
    20341 acctggccta cctgcacgcc gtgaagaatg agcggctgac ccgcatcaat accgtaactt
    20401 tccggcgcgg tcagggtcag cgtcagaaca tcaggttcat gcagcagtgc tttttcgcct
    20461 ggcacaacaa cgaactcttt gcgtggctcg ccatcgccgg gtaccaactc gaaggttttg
    20521 ccagtcagca gggcgctgag attggcatcg ctaagggata atttcgggtt gcgtaattcg
    20581 atgcgggtat tttcccgtaa cagcgtaacg acgctgggat caacggtcat ttctccggtg
    20641 actttaccac caggatttaa atccagttta gttagctgtc cgacttccag tccctgatac
    20701 attaatggcg tcgagtcggc ggttaatccg gccccactcg gcagttccag tttgattatt
    20761 acgccacgct ggctatgggc gaggtcttca tacagaccaa aggtatcttc cgcctcggca
    20821 ggtttcgact cttctggtga atcgaaggca atcgcaccgt taaccagtgc cgccaggctt
    20881 tccagtttca ccttcgcgcc actgatactg acattggcat caacgccgga aacgttccag
    20941 aaacggctac cttttttcac cagatcggta aaacgccgct cgatcaggac atcaatcacc
    21001 acgccttgtt tgttgggatt gatggcatag tcgtagactt ttcccaccgg gatcttgcgg
    21061 aaatagacca atgaaccgct gttcagcgaa ccgagatcgg ggacttgcag gtggatcatc
    21121 agatcgccat tgtccagccg atatttcggt tgggtatcga gtgcgacaaa gtgatcctgc
    21181 tctttacctt tacccggcat cataccgata tagttcccac cgacgagggc gtccagcccg
    21241 gagacacctg ccaacgatgc ttttggcgtc accagccaga actgagtctc ttcgcgcagc
    21301 gcatctttca tatcggactt gatgctgacc ttgacttcaa tcttacgaag atcgtcgctg
    21361 aggctgatat cctgcactgt tccgacttca acgccctgat aacgaacagg cgtacggccc
    21421 gggacaatac catccgccga cataaagtcg atggtgacgg tattaccccg gtcctgataa
    21481 ctgtcccaaa tcagccaacc ggcaatcatt agcgcgatga aaggcagcag ccagaaaggt
    21541 gagatacggc gtttattttt aatctgcgct tcagtcgtcg aagcgggcgt ttcctgactc
    21601 atgtgcatcc caaagtaagc ggctgtccag ccattccaca gcaagaatag tcaaaattac
    21661 ggctgcgcca aaataaaacg cagccggtcc catagtaaaa gcgaggatct gatcgcgatt
    21721 aatcagcgac atggttaaag atatgacaaa caggtcgagc atcgaccagc gtccaatcca
    21781 ggtcaccatc cgcagtaaca gaatgcgtgt gcgtaaacct tgctggcatt taaaatgaat
    21841 gctcaacagt aaagtgaaca tgacgatcac tttagtaaac ggtaccagaa tactggcgat
    21901 aaacacgatt ccggcgaccg caatgttgct acttgccagc gacataatcc cggaaagaat
    21961 tgtatcttcc tgccgtcctc cgttcagata aatgatagaa ataggcaaca ggttggctgg
    22021 taacaacaaa acgattgacg ctaacagcgc cgcccagcat ttttgcagac tatgacggcg
    22081 acgcaggcgt agcgggatat ggcaacgcgg gcagcgacca cgctgatcag gatagccggt
    22141 aaaatggcac ccaagacaga cacggagttt ctcgtcccta cgcgtagcgg ggcgctgcgg
    22201 ataaaatcgc tcccacagct cctcgacatt aagatgtgac aacgtcaccg tcgttaaaat
    22261 caccaacgcc acaaaagaga acaagcccac acccgcctgg atatgggcat aatcctgtac
    22321 ctttatagaa gcaacgccaa tgccgaccag gtagatatcc agcattaccc actctttaag
    22381 tcgctcaagc atcagcagta ccaggcgtag attcattccc agtcggttac caaaccataa
    22441 ataagctatg gaggtcacca gaatgagggg ggcaccgata acgcagaaaa agaccatcga
    22501 ccccgttatc gcatcgccct gtttggtcat ttgccagatg ccttgcataa cgttggcgtc
    22561 aatacgaatg cctaacagcc agatatgcaa cagcggttcg ccccaggcaa acggcatcag
    22621 caacagcatg gtgaaagcca ttgccgccag gcgcgttagc gaccagtcgc gcccgtcgcg
    22681 aatttttgcc tgacagcgcg gacaataggc actctgatga gaatttatct cgggcaggct
    22741 aaacagcatg tcacattgcg ggcaacgttg gtaatcacca cgcggcagtt cctcgccgat
    22801 tgacctcact tttatctttt ttgtcggcgt aatttgtggt gtgttaagag ccatgacgtg
    22861 ccaaatgtaa taagaattaa cattaatatt aacccatgat tggaatgatc ttgagcatta
    22921 acgcatttaa tgcttatttt aataggctgg cagttccagg taagacaacc aagtgattat
    22981 ataatgaaca aaacagaatt ttacgcggat ttaaatcgcg actttaacgc gctgatggcg
    23041 ggagaaacca gttttctggc aacgcttgcg aacaccagtg cgttgttata cgagcgtctc
    23101 actgacgtaa actgggcagg tttttatttg cttgaggacg atacactggt actcggacca
    23161 tttcagggca aaattgcctg tgtccggata cccgtcgggc gcggggtgtg cggcactgcg
    23221 gttgcccgca atcaagtgca gcgtatcgag gatgttcacg cgtttgacgg gcatattgcc
    23281 tgtgatgcgg cgagtaattc tgaaattgtt ctgccgctgg tggtgaaaaa tcagattatt
    23341 ggtgttcttg acatcgatag taccgtcttc ggtcgcttta cagacgagga cgagcaaggc
    23401 ttacgtcagc ttgtggcaca gcttgaaaaa gtgcttgcaa cgacggatta caaaaaattc
    23461 tttgcgagcg tcgcaggata atcaacggat aacgtagcat ttactggtag cgtcattata
    23521 atgacgcctg ttcatgcctg cgcttgttgg ctacgtccgt tgtaatcagg aaatttcatg
    23581 gaaaatcaac ctaagttgaa tagcagtaaa gaagtaatcg cgtttctggc cgaacgtttt
    23641 ccccactgtt tcagtgcgga aggtgaagcg cgtccgctga aaatcggtat ttttcaggat
    23701 ttggtcgatc gtgttgccgg ggaaatgaac ctgagcaaaa cgcaattacg atccgcttta
    23761 cgtctctaca cttcgagctg gcgttatctt tacggtgtta aacccggcgc aacgcgtgtc
    23821 gatcttgacg gcaacccatg cggtgagctg gacgagcaac atgtagagca tgctcgcaag
    23881 cagcttgaag aagcgaaagc gcgtgttcag gcacagcgtg ctgaacagca agcgaaaaaa
    23941 cgcgaagctg ccgcagctgc tggtgagaaa gaagacgcac cgcgccgcga acgcaagcca
    24001 cgtccgacca cgccacgccg caaagaaggc gctgaacgta aacctcgtgc gcaaaagccg
    24061 gtagagaaag cgccaaaaac agtaaaagca cctcgcgaag aacagcacac cccggtttct
    24121 gacatttcag ctctgactgt cggacaagcc ctgaaggtga aagcgggtca aaacgcgatg
    24181 gatgccaccg tattagaaat caccaaagac ggcgtccgcg tccagctgaa ttcgggtatg
    24241 tctttgattg tgcgcgcaga acacctggtg ttctgaaacg gaggccgggc caggcatgaa
    24301 catgtttttt aggcttaccg cgttagctgg cctgcttgca atagcaggcc agaccttcgc
    24361 tgtagaagat atcacgcgtg ctgatcaaat tccggtatta aaggaagaga cgcagcatgc
    24421 gacggtaagt gagcgcgtaa cgtcgcgctt cacccgttct cattatcgcc agttcgacct
    24481 cgatcaggca ttttcggcca aaatctttga ccgctacctg aatctgctcg attacagcca
    24541 caacgtgctg ctggcaagcg atgttgaaca gttcgtgaaa aagaaaaccg agttaggcga
    24601 tgaactgcgt tcaggcaaac tcgacgtttt ctacgatctc tacaatctgg cgcaaaagcg
    24661 ccgttttgag cgttaccagt acgctttgtc ggtactggaa aagccgatgg atttcaccgg
    24721 caacgacact tataaccttg accgcagcaa agcgccctgg ccgaaaaacg aggctgagtt
    24781 gaacgcgctg tgggacagta aagtcaaatt cgacgagtta agcctgaagc tggcaggaaa
    24841 aacggataaa gaaattcgtg aaaccctgac acgccgctac aaatttgcca ttcgtcgtct
    24901 ggcgcaaacc aacagcgaag atgttttctc gctggcaatg acggcgtttg cgcgtgaaat
    24961 cgacccgcat accaactatc tttccccgcg taataccgaa cagttcaaca ctgaaatgag
    25021 tttgtcgctg gaaggtattg gcgcagtgct gcaaatggat gatgactaca ccgttatcaa
    25081 ttcgatggtg gcaggtggtc cggcagcgaa gagtaaagct atcagcgttg gtgacaaaat
    25141 tgtcggtgtt ggtcaaacag gcaagccgat ggttgacgtg attggctggc gtcttgatga
    25201 tgtggttgcc ttaattaaag ggccgaaggg cagtaaagtt cgtctggaaa ttttacctgc
    25261 tggtaaaggg accaagaccc gcactgtaac attgacccgt gaacgtattc gtctcgaaga
    25321 ccgcgcggtt aaaatgtcgg tgaagaccgt cggtaaagag aaagtcggcg tgctggatat
    25381 tccgggcttc tatgtgggtt tgacagacga tgtcaaagtt caactgcaga aactggaaaa
    25441 acagaatgtc agcagtgtga tcatcgacct gcgtagcaat ggcggtgggg cgttgaccga
    25501 agcggtatcg ctctcaggtc tgtttattcc tgcgggtccc attgttcagg tccgcgataa
    25561 caacggcaag gttcgtgaag acagcgatac cgacgggcag gtgttctata aaggcccgct
    25621 ggtggtactg gttgaccgtt tcagtgcttc ggcttcagaa atctttgccg cggcaatgca
    25681 ggattacggt cgtgcgctgg ttgtgggtga accgacgttt ggtaaaggca ccgttcagca
    25741 ataccgttca ttgaaccgta tttacgatca gatgttacgt cctgaatggc cagcgctggg
    25801 ttctgtgcag tacacgatcc agaaattcta tcgcgttaac ggcggcagta cgcaacgtaa
    25861 aggcgtaacg ccagacatca tcatgccgac gggtaatgaa gaaacggaaa cgggtgagaa
    25921 attcgaagat aacgcgctgc cgtgggatag cattgatgct gcgacttatg tgaaatcagg
    25981 cgatttaacg gcctttgaac cggagctact gaaggaacat aatgcgcgta tcgcgaaaga
    26041 tcctgagttc cagaacatca tgaaggatat cgcgcgcttc aacgctatga aggacaagcg
    26101 caatatcgtt tctctgaatt acgctgtgcg tgagaaagag aataatgaag atgatgcgac
    26161 gcgtctggcg cgtttgaacg aacgctttaa acgcgaaggt aaaccggagt tgaagaagct
    26221 ggatgatcta ccaaaagatt accaggagcc ggatccttat ctggatgaga cggtgaatat
    26281 cgcactcgat ctggcgaagc ttgaaaaagc cagacccgcg gaacaacccg ctcccgtcaa
    26341 gtaatatcaa tcaggcacaa gaaattgtgc ctgatttttt aacagcggca agatgccgta
    26401 aatcagatgc tacaaaatgt aaagttgtgt ctttctggtg acttacgcac tatccagact
    26461 tgaaaatagt cgcgtaaccc atacgatgtg ggtatcgcat attgcgtttt gttaaactga
    26521 ggtaaaaaga aaattatgat gcgaatcgcg ctcttcctgc taacgaacct ggccgtaatg
    26581 gtcgttttcg ggctggtact gagcctgaca gggatacagt cgagcagcgt tcaggggctg
    26641 atgatcatgg ccttgctgtt cggttttggt ggttccttcg tttcgcttct gatgtccaaa
    26701 tggatggcat tacgatctgt tggcggggaa gtgatcgagc aaccgcgtaa cgaaagggaa
    26761 cgttggctgg ttaatactgt agcaacccag gctcgtcagg cagggatcgc tatgccgcaa
    26821 gtggctatct accatgcgcc ggacatcaac gcttttgcaa ccggtgcgcg ccgtgatgcc
    26881 tctctggttg ctgtcagcac cggtttgctg cagaacatga gcccggatga agccgaggcg
    26941 gtaattgctc acgaaatcag ccacatcgcc aatggtgata tggttaccat gacgctgatt
    27001 cagggcgtgg tgaacacctt cgttatcttt atttcccgta ttctggcgca gcttgccgcg
    27061 ggttttatgg gcggaaatcg tgatgaaggt gaagagagca acggcaaccc gctgatctac
    27121 tttgcggttg caacggttct ggaactggtg tttggtattc tggcgagcat tatcaccatg
    27181 tggttctcgc gtcatcgtga attccatgct gatgccggtt cggcaaaact ggttggtcgc
    27241 gagaaaatga ttgccgcgct gcagcgcctg aaaaccagct atgaaccgca agaagcaacc
    27301 agcatgatgg ctttctgcat taacggtaag tcgaaatcgc tcagtgagtt gttcatgact
    27361 cacccgccgc tggataaacg aattgaagct ctgcgtacgg gtgaatacct gaagtaatat
    27421 gaagtaaata ctaaaaagcg cgtcctgatc gacgcgcttt ttttatgccc tggatcgtgg
    27481 ctgagtgata cgtaaaccac tcacacaggc agcaatcact gccagaatag ccgcagccat
    27541 cagcgaaacg tgcgtaccat tatcaccgaa ctgatttagc atcagcgcca ccagcgccgc
    27601 gccactactc tgacccagta gacgagccgt tcctaacatg ccactggccc caccgctacg
    27661 ttcgcgcgga gcggaggtaa taatggtgtg gttattgggt gactggaata agccaaatcc
    27721 agcgccacat aagatcatcg gccagataat attgatatcc gcaggtgacg cgggcagcag
    27781 aaccagggaa aaaagccccg cagccatgat gaacaaccct aaagcaccca gcaatcctgc
    27841 atgtacgcgt tcaatcaaat agcctgccag cggagccatc accatcgttg ctaacggcca
    27901 cggtgtcaga agtaaacctg tttcgacttc actacgcccg agcacggttt gcaggtaaaa
    27961 gggcagggaa accattgcca gcatttgtgc gcagaaagag caaacagatg tgcaaataga
    28021 aagtgaaaac agcgggatac gcagtaaatc caccggtagc agcggtacgg gaagagaaag
    28081 ctggcggcga ataaagaaaa taccaacaac aaccattacc accagttccg cagcaattaa
    28141 cgtcagcgat tgcccctgag cgaaaccact caacgcagtg ataagcaggc cgaaggttaa
    28201 cgcgttcatc acggcgctgg gcaggtcgaa acggggttta ctggcgcgag aaccattggg
    28261 tggcagaaaa cgcatcgcca gaagcagggc gataatacct aacggtacgt tgattaaaaa
    28321 taaccatttc caggatgcga tggagaggat tgctgcagca attgtcggcc cggcagcaga
    28381 agagacggca acaataaacg agtttatgcc catccctctc cccagaaaac gttgtggata
    28441 gatcaggcgg ataagtgcgg tattaacgct catcaacgct gcaccgccga aaccttgtat
    28501 gacacgcgca agggtgagca tttgcagcga atcagaaagg gcgcagaaca gtgaagacaa
    28561 cagaaaaacg accagaccgc atttataaat acgtcgatag ccaaacatat cgcccagaaa
    28621 cgaaaacgag agcagggaga tgacaatggc gatttgatag gcgttcacta cccagatgga
    28681 actggctggc gtggcatgaa ggtccgtggc gattgttggc agggcgacgt tggcgattgc
    28741 gccgtcaagg acagccatcg aaatgccaat cacaatggtt aatatcgcac cgtatcgctg
    28801 gggcaatggc aggccgtcgg cctgaacttt tggcatagga attttatatc tttggtgaat
    28861 aatcaacagg ggtatgattc tagcactgat attcacggtg gatgtcgcag aattgtaact
    28921 aaccggcaaa agatggattg cgaccaccct ggtgcgaaat tataataaaa aacagttctg
    28981 atttttataa aacactcgca atgaggtgat aaatggctaa cgcagatctg gataaacagc
    29041 ctgattctgt atcttccgtg ctaaaagttt ttggcatttt gcaggcgctg ggtgaagagc
    29101 gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat gtcaaaaagc accgtttatc
    29161 gctttttaca gaccatgaaa accttaggtt atgtggcgca ggaaggggag tcggagaaat
    29221 attccctgac cctgaaattg tttgaactgg gcgctcgcgc gttacaaaac gtcgatttaa
    29281 ttcgtagcgc agatatccag atgcgtgaga tctcccgcct gaccaaagaa actatccacc
    29341 tcggcgcact ggacgaagac agtattgttt acattcacaa aattgactct atgtacaatt
    29401 tgcgcatgta ttcacggatt gggcgtcgta atccgctgta cagcaccgcg attggtaagg
    29461 tactgctggc atggcgcgat ctcgatgaag tgaagcaaat tcttgagggc gtggagtata
    29521 aacgcagtac cgagcggacc atcaccagta cagaagcgtt attacccgtt ctggaccagg
    29581 tgcgcgagca ggggtatggc gaagataacg aagagcagga agaagggctg cgatgcattg
    29641 cggtaccggt atttgatcgc tttggtgtgg tcattgccgg tttgagcatc tccttcccga
    29701 cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc aatgttgcat accgcagcgc
    29761 gcaaaatttc tgcccaaatg ggttatcacg actatccgtt ctgatgagag taagaacctg
    29821 tcggaatatc aaacagacag gttctttatt tagcatgaga aaaataaagt tgaaggtgcg
    29881 ttatattaaa cgcgcttgct ataagaatat tttactcatg agtgagaatc tggttgttta
    29941 ttacccttaa ccattatcga ccacgatatt gcttttacgt aacagtgggc aatctgttat
    30001 ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg ctggtgcctc tggcagtcag
    30061 ataggtacat tgcaaaccta atcctgcggc attctctttg cttccaatca aaacgccata
    30121 tccgctgagt aataatccta tccataccag tgctatcagc ataactgtgc gaatgatgaa
    30181 tcgcattaca acctcttctc tttttatgtt cgcttaatcg tagcggcaat atgcgctgaa
    30241 gcaagcgact cattccgaaa aagcacgaat atcgacatag ttaggcgctg tttaactaac
    30301 gcatgctagt ttaatgacat aaggtaggtg aaacggagat tggagtgaaa aagtttcgat
    30361 gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct ttgggcgcag gtattcaaca
    30421 tgatgtgcga tcaggatgta caatttttca gcggaatttg tgccattaac cagtttatcc
    30481 cgtggtgata tcattttttc agcactagat tttcttcctc tccagcagtg gtagaatagc
    30541 gcctctactc agaggtggtg aaatgaacga agttgtaaat tcaggcgtga tgaacattgc
    30601 gtctttggtt gtatcggtgg tggttcttct tatcgggctc atcttgtggt tttttatcaa
    30661 tcgtgccagt tcacggacta acgaacagat tgaactgctt gaggcgttgc tggatcagca
    30721 aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca aacgaaccag agaaagcaga
    30781 taaaaagacc gttgagagtc aaaaatcggt tgaagacgaa gatattattc gtctggtcgc
    30841 cgaacgataa tccttcctca atcttgagta accccgtcgt tgaagggtta ctcaattcat
    30901 cctgattata cgcactgcca tggtgcaact gcttcaaatt aaaactctgg tctgacttca
    30961 a
//
LOCUS       DAJSKJ010000024        30855 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000024 DAJSKJ010000000
VERSION     DAJSKJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.024"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ON345_000988"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ON345_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR3071086.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ON345_000989"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ON345_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR3071087.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ON345_000990"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ON345_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR3071088.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ON345_000991"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ON345_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR3071089.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ON345_000992"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ON345_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR3071090.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ON345_000993"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ON345_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR3071091.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ON345_000994"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ON345_000994"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3071092.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ON345_000995"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ON345_000995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR3071093.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /locus_tag="ON345_000996"
     CDS             12676..13215
                     /locus_tag="ON345_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCR3071094.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ON345_000997"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ON345_000997"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR3071095.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ON345_000998"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ON345_000998"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR3071096.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ON345_000999"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ON345_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR3071097.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLNQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ON345_001000"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ON345_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR3071098.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ON345_001001"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ON345_001001"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR3071099.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ON345_001002"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ON345_001002"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR3071100.1"
                     /translation="MSATKLTRREQRAHAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19069..19169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19221..19697)
                     /gene="rsd"
                     /locus_tag="ON345_001003"
     CDS             complement(19221..19697)
                     /gene="rsd"
                     /locus_tag="ON345_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR3071101.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19792..20565
                     /gene="nudC"
                     /locus_tag="ON345_001004"
     CDS             19792..20565
                     /gene="nudC"
                     /locus_tag="ON345_001004"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR3071102.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20605..21669
                     /gene="hemE"
                     /locus_tag="ON345_001005"
     CDS             20605..21669
                     /gene="hemE"
                     /locus_tag="ON345_001005"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR3071103.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLREKDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21826..22497
                     /gene="nfi"
                     /locus_tag="ON345_001006"
     CDS             21826..22497
                     /gene="nfi"
                     /locus_tag="ON345_001006"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR3071104.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22540..23130
                     /locus_tag="ON345_001007"
     CDS             22540..23130
                     /locus_tag="ON345_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR3071105.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23317..23589
                     /gene="hupA"
                     /locus_tag="ON345_001008"
     CDS             23317..23589
                     /gene="hupA"
                     /locus_tag="ON345_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR3071106.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23602..24297
                     /locus_tag="ON345_001009"
     CDS             23602..24297
                     /locus_tag="ON345_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR3071107.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24299..24724)
                     /gene="zraP"
                     /locus_tag="ON345_001010"
     CDS             complement(24299..24724)
                     /gene="zraP"
                     /locus_tag="ON345_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR3071108.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24962..26338
                     /gene="zraS"
                     /locus_tag="ON345_001011"
     CDS             24962..26338
                     /gene="zraS"
                     /locus_tag="ON345_001011"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR3071109.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26335..27660
                     /gene="zraR"
                     /locus_tag="ON345_001012"
     CDS             26335..27660
                     /gene="zraR"
                     /locus_tag="ON345_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR3071110.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVDALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27657..28946)
                     /gene="purD"
                     /locus_tag="ON345_001013"
     CDS             complement(27657..28946)
                     /gene="purD"
                     /locus_tag="ON345_001013"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR3071111.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERDSLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28958..30547)
                     /gene="purH"
                     /locus_tag="ON345_001014"
     CDS             complement(28958..30547)
                     /gene="purH"
                     /locus_tag="ON345_001014"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR3071112.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7285 a         8000 c         8425 g         7145 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctattg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcactgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aagtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattact cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gttttttgcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgcttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgaa ttgcgaggca tttagcgccc gctctacgtc agcagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccgg tcagcgggct ggtagcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggaac
    15001 tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa
    15241 ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc
    15301 cgcggttttc gccccggaag tattcggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcgaatag actccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcctgc ttggcgttga ttgagctgct
    15661 ccagcagctc gtgaaccgtt tgtccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctag acttaccatc gaacagtcgg agttctcccg caggcgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtaccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa aatcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgtgt cgaaacaccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16861 atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 cactgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctggacc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggcagggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cacggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgaacgccg gggtgcgtgc cagtgatata aatacgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcgtg ggcgcgctgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaagttaagg acgtggcttg
    19021 tcagacgacg gatgaagcaa gagacgatcg ccgcaaaggc gatgcgatag cagattaact
    19081 cttgttccct tcgcaggtat tagcctgatc aggttccgcg gatcccgaat aaacggtctc
    19141 agccagttaa ggcactccga caagaaatta gccccgcaaa tggggcattg aatgtaaatt
    19201 acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc aagcaccagc
    19261 agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat gtcagataaa
    19321 acctgttgaa actcaaggta attatcatga tcgatggcgg tttccaggct ggaatcgtag
    19381 taatccataa tctgttgggt attggcttcg agttgcggcc aaatcttcgc ggcgcgcgcg
    19441 agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa atgtccggca
    19501 gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt tagcctcatg
    19561 tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag cagatgctta
    19621 cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc cgtcaggtta
    19681 tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc tcagtgagaa
    19741 atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc tatggatcgt
    19801 ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa attatggttg
    19861 ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg tcagcgcgca
    19921 ctacaaatcg gcgaatggca gggggaacct gtttggttag tacaacagca gcggcgtcac
    19981 gatatggggt cggtacgtca ggtcattgat ctcgatgttg ggctgtttca actggccgga
    20041 cgaggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta ctgcgggcat
    20101 gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg tgagcgttac
    20161 tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc gatcctcctc
    20221 gcccagcata cccgccatcg taacggtgtc catacagtac ttgccggatt cgtcgaagtg
    20281 ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg aagagagcgg catcaaagtt
    20341 aaaaacttgc gttacgtgac ttctcagccg tggccgtttc ctcagtcttt aatgaccgcg
    20401 tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt gctcgaggcg
    20461 aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt agcgcgccgt
    20521 ctgatagaag atactgtggc gatgtgtcgg gcagagtatg agtgatgata cactgaccgc
    20581 ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta tctgcgggcg
    20641 ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc gggtcgctat
    20701 ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct gtgcaaaaac
    20761 gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct ggatgcggcg
    20821 atcctctttt ccgacatact taccgtgccg gacgcgatgg ggttagggct ctattttgaa
    20881 gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt cgataaactg
    20941 ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg taccattcgt
    21001 cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg gacgctggca
    21061 acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa aatgatgtat
    21121 gccgatccgc aggcgctgca cgctctgctc gataaactgg cgaaaagcgt cactttgtat
    21181 ctgaatgcgc agattaaagc cggtgctcag gcagtgatga ttttcgacac ctggggcggt
    21241 gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt acatgcataa aattgttgat
    21301 ggtttactgc gtgaaaaaga cggtcgccgc gtaccggtca cgctgtttac caaaggcggc
    21361 ggacagtggc tggaagccat ggcagaaacc ggttgcgatg cgctgggcct cgactggaca
    21421 acggacatcg ccgatgcgcg ccgccgcgtg ggcaataaag tcgcgttgca gggcaatatg
    21481 gatccgtcga tgctgtacgc gccgccagcc cgcattgaag aagaagtagc gactatactt
    21541 gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat tcatcaggat
    21601 gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc tgaacagtat
    21661 caccgctaag agcctggtcc atcaggctat tttatttgcc attttgaggc cgggcagtgc
    21721 tcaaaatcct cacgtactac gtgtacgctc cggtttttcc gcgctgtccg gcctcaaact
    21781 gccttcaaca attacgcctg ttgaaccagg ctctaaggag cgattatgga tctcgcgtca
    21841 ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg actcgataaa
    21901 gatccaccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg agaagtgacg
    21961 cgagcggcga tggtgctgct gaaatatccc tcgcttgagc tggtggagta taaagttgcc
    22021 cgcatcgcca ccaccatgcc ttacattcca ggttttcttt ccttccgcga atatcctgcg
    22081 ctgctggctg cgtgggagat gctgtcgcaa aagccggatt tagtgtttgt cgatggtcat
    22141 gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggcttatt ggtggatgtg
    22201 ccgaccattg gcgtggcgaa aaaacggctc tgcggtaaat tcgaaccgct ctccagcgaa
    22261 ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt ctggcgcagc
    22321 aaagcgcgct gtaacccgtt gtttatcgct accggccatc gggtcagcgt ggacagcgcg
    22381 ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac gcgctgggcg
    22441 gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca gccctaattc
    22501 aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa cccaattcat
    22561 ctgcgtctgg agcgcctgga aagctggcag catgtcactt tcatggcttg cttatgcgaa
    22621 cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga tgggcaaatt
    22681 taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagacgc aaaagtaaat
    22741 ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga tttcgatctg
    22801 tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt ccattcgcgt
    22861 ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat cacgaccgtt
    22921 gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct caaagaaaac
    22981 ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt agctgagtgc
    23041 gaagaacgcg acatcgagct gataaaaggc cttagggcag acctgcgtga ggcgggtgag
    23101 agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa cgcctgattt
    23161 gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg ggcgaaaaaa
    23221 agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt cgcactcgat
    23281 gcttagcaag cgataaacac attgtaagga taacttatga acaagactca actgattgat
    23341 gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct ggagtccact
    23401 ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt tggtttcggt
    23461 accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac cggtaaagaa
    23521 atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact gaaagacgca
    23581 gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc accccttttg
    23641 tctttctggc gtcgatcatt gatgctggca ggcgctctgt ttctcactgc ctgtagtcac
    23701 aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg cgcggtacgc
    23761 atctggcgaa aagacagcgg cgataatgtg catctgcttg ccgtgtttag cccgtggcgc
    23821 agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac gctcatcaat
    23881 atcaatgttt acagcaaacc gccggtgaac attcgcgcgc gttttgacga tcgcggtgat
    23941 ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa cgaccaaatc
    24001 gatttatacc gttatcgtgc tgatcagatc cgccagatta gcgatgcctt acgtcagggg
    24061 agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac cacctgcgaa
    24121 gggcaaacca ttaaacctga tttagattcg caggcgatag cgcatatcga gcgccgccag
    24181 agccgctctt ctgttgatgt cagtgtggca tggctggaag cgcccgaagg ttcgcaatta
    24241 ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac gttctgattt
    24301 accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc atgcccattc
    24361 cggctccgcg cggaataccc gcttcagcca tcgcaatatc ccgtttcacc cgtaactcat
    24421 ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg ctgctatccg
    24481 gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt tgctgttgca
    24541 gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc tgttgttcgc
    24601 tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca aatgcagatg
    24661 tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc gtgttccgtt
    24721 tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg tgccaacgat
    24781 gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac tcgcctgctg
    24841 cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacaa cgcagggtaa
    24901 gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg caaagaagaa
    24961 gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg cgatcctccc
    25021 cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg attatgggcg
    25081 cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta ttcgcgctct
    25141 tgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc agcaggcctt
    25201 actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg atgaacaagg
    25261 aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt ccccgcagga
    25321 aatgcagcag ttacatcctg gagatgaaga agcgtggcgg cggatcgata gcgcagacgg
    25381 cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg gaatgcaccg
    25441 gatgcgccat atgcagcagt atgccgcaac accacaagca attttcatcg ctttcgacgc
    25501 cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta tcctcttcgc
    25561 cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc gctatctgcg
    25621 ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg cgctggggca
    25681 tcttgcggca ggcgttgccc acgaaatccg taacccactt tcctcgatta aaggactggc
    25741 gaaatacttt gccgaacgcg cgccagcagg gggagaagcg catcaattgg cgcaggtgat
    25801 ggcgaaagaa gccgaccgtt taaaccgcgt ggtaagcgag ttgctggaac tggttaagcc
    25861 aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact cattacagct
    25921 ggtaagccag gatgcaaaca gccgggagat ccagttacgc tttaccgcca acgacacatt
    25981 accggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc tctatctcaa
    26041 tgctattcag gcgattggtc agcatggcgt gattagcgtg acggtcagcg aaagcggcgc
    26101 gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc agcttgaagc
    26161 catcttcact ccgtacttca ccaccaaagc cgaaggcacc ggattggggc tggcggtcgt
    26221 gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagccagg agggaaaagg
    26281 ctcaacgttc accctctggc ttccggtcaa tattacgcgt aaggacccac aaggatgacg
    26341 cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac tattttgcag
    26401 gctttactgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg acaggcgctg
    26461 gagcaggtgc gggaacaggt ttttgatctt gtgctttgcg atgtgcgaat ggcggagatg
    26521 gacggcatcg ccacgctgaa agagatcaaa gcgttaaacc cggcaattcc ggtgctgatt
    26581 atgactgcgt actccagcgt cgagacggcg gtagatgcac tgaaaactgg ggcgctggat
    26641 tatctcatca agccgctgga tttcgataac ctacaggcga cgctggaaaa agcgctcgcg
    26701 catacgcaca gtattgatgc tgaaacacct gcggtgactg ccagccagtt cggtatggtc
    26761 ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc gccatcggaa
    26821 gccacggtac tgatccacgg cgattccggc accggtaaag agctggtcgc cagggcgatt
    26881 cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc ggcactcaac
    26941 gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac tggagccgat
    27001 aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgctgtttct cgatgaaatt
    27061 ggcgatatct cgccgatgat gcaggtgcgt ctgctgcgtg cgattcagga gcgcgaagtt
    27121 cagcgtgtcg gtagcaacca gactatctcg gttgatgtcc ggctgattgc ggcgacccat
    27181 cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta ctatcgcctg
    27241 aatgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat ccctctgctg
    27301 gctggccatt ttctgcagcg ctttgccgag cgtaatcgca aggcggtaaa aggttttacg
    27361 ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg tgagctggaa
    27421 aacgcggtgg aacgggcagt ggtgctgctg accggggaat atatttccga acgcgagctg
    27481 ccgctggcga ttgccagtac gccgatcccg ctgggacaaa gtcaggatat tcagccgttg
    27541 gtggaagtgg aaaaagaggt gattctggcg gcgctggaga aaacgggcgg caacaaaacc
    27601 gaagccgccc gtcagttagg gatcacgcgc aaaacgctat tggcaaaact gtcgcgttag
    27661 ttctgctcgc gttcgatagc gcgccagccg atatctttcc ggcagaagca gtcgtcccag
    27721 tggatatcgg tcattaaggc ataggcgcgt ttctgcgctt ctgccacggt atgacccagc
    27781 gcggtgacgc acagtacgcg cccgccgttg gtcactacct gctcgtcatc cgccagtttt
    27841 gtgcccgcgt ggaacacttt gccgcctgcc acttcttcca gcggcaggcc gtggatcacg
    27901 tcaccggtgc ggtaatcacc cggatatcca cccgcagcca tcaccacgcc gagagaatcg
    27961 cgttcatccc actcggacgt tttctcgtcc agtttgcttt cacaggccgc caggcagagc
    28021 tcaaccagat cggacttcat gcgcagcata atcggctggg tttccggatc gccaaagcgg
    28081 cagttaaatt caataacctt cggattgccc tgtttatcga tcatcaggcc cgcgtagaga
    28141 aaaccggtgt aggtgttgcc ttccgccgcc atgcctttca cggttggcca gataatacgt
    28201 tccatggtgc gctgatgaac gtcatcggtt actaccggcg cgggggagta agcgcccatc
    28261 ccgccggtgt ttggtccggt atctttatcg cctacgcgtt tgtgatcctg gctggtagcc
    28321 atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc gccatcgagg
    28381 aactcttcga taacgatgcg atgacccgcg tcgccaaaag cgttgcccgc cagcatatcg
    28441 tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt cccggcagcc
    28501 agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc cagcgcaggt
    28561 tctacctcgg tgaagttctg gtattccgcc gtagggatct tatggcgggc caggaaatct
    28621 ttggtaaacg cttttgagcc ttccagttgg gccgcacctg cggttgggcc gaagattttc
    28681 agcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc cgggccgacg
    28741 atggtcagat caatcttttc gttttgtgcg aaatccaaca gcgccgggat atcggtcacg
    28801 ccaatagcaa cgttttgcag cgcgggttcc agtgcagtgc ctgcattacc cggagcaaca
    28861 aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg ctcgcgcccg
    28921 ccgttaccaa tcactaatac tttcatctat tgctccatta atggcggaag tggcgcatgt
    28981 cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg tcatcacgga
    29041 tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca tcaataccgt
    29101 cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc aggccttcat
    29161 cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg cccgcgccaa
    29221 taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg aacttcgcca
    29281 ctttccagca gaacagcgca tcgcgcagtt cctgttcggt cggctgacgt tgggtgacga
    29341 cgcgcagttc ttccgcaccg accatcccca ggtcacgatc ctgaaccagc agaccgccgt
    29401 tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag gtcagaacgc
    29461 gtacgttctg cttggcggcg gtgattttca gggcttcttc gctggcggaa ggcgcaataa
    29521 tcacttcgac aaactgacga gaaatgatgg cctgtgcggt ttccgcatct agctcgcggt
    29581 taaaggcaat gatgccgccg aatgcggagg ttgggtcggt tttgtacgcg cgatcgtaag
    29641 catcaagaat ggaattgccg atagctacac cgcaagggtt ggcgtgcttc acaatcacac
    29701 atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg gcgatgttgt
    29761 tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct tctttcacat
    29821 tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc atatcctgct
    29881 tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct ttgctttcac
    29941 cgtggtacgc cggaaccatg ctgccgaagt agttggcaat catgctgtcg taggcggcag
    30001 tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc gatccttcgt
    30061 tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg attgcgacat
    30121 ctttatggtt cttagcggcg gagcgcacca tggtcgggcc gccgatatcg atgttctcaa
    30181 ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga tacaggttaa
    30241 caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg tcctggccgc
    30301 gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt ccatccatca
    30361 tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt tctgctaaca
    30421 gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt gcctgggcga
    30481 attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact ggacgacgtt
    30541 gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga atttttcgcg
    30601 aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat tgtaacgaaa
    30661 acgtttgcgc aacgctcgcg aatttttctc tttcaatggt gatcacaatt ttgacagtgg
    30721 ttaccgtggg aaaaatacag aaattacatt gatgattgtg gataactctg tgcgtaaaaa
    30781 ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg caatttttct
    30841 attgcggcct gcgga
//
LOCUS       DAJSKJ010000025        30547 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000025 DAJSKJ010000000
VERSION     DAJSKJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.025"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ON345_001015"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ON345_001015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR3071113.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSVWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ON345_001016"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ON345_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR3071114.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ON345_001017"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ON345_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR3071115.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="ON345_001018"
     CDS             3807..3995
                     /locus_tag="ON345_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR3071116.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="ON345_001019"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="ON345_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR3071117.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..7374
                     /gene="bcsA"
                     /locus_tag="ON345_001020"
     CDS             4756..7374
                     /gene="bcsA"
                     /locus_tag="ON345_001020"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR3071118.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTYWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7385..9724
                     /gene="bcsB"
                     /locus_tag="ON345_001021"
     CDS             7385..9724
                     /gene="bcsB"
                     /locus_tag="ON345_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR3071119.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGTQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTLMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9731..10837
                     /gene="bcsZ"
                     /locus_tag="ON345_001022"
     CDS             9731..10837
                     /gene="bcsZ"
                     /locus_tag="ON345_001022"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR3071120.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10819..14280
                     /gene="bcsC"
                     /locus_tag="ON345_001023"
     CDS             10819..14280
                     /gene="bcsC"
                     /locus_tag="ON345_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HCR3071121.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LETANRLRETNRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARAAYQN
                     VLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQAQLGD
                     TAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVASGVT
                     TTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGTGGYS
                     DLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDCSGNR
                     SQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAHRRPI
                     SSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTGKNVE
                     DNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEYLSFA
                     IPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDGGSSQ
                     GFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDLPPQP
                     LIPYADW"
     gene            14362..16350
                     /gene="hmsP"
                     /locus_tag="ON345_001024"
     CDS             14362..16350
                     /gene="hmsP"
                     /locus_tag="ON345_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR3071122.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQVAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16532..17818
                     /gene="dctA"
                     /locus_tag="ON345_001025"
     CDS             16532..17818
                     /gene="dctA"
                     /locus_tag="ON345_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR3071123.1"
                     /translation="MKTSLFKRLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18038..19534
                     /locus_tag="ON345_001026"
     CDS             18038..19534
                     /locus_tag="ON345_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3071124.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEVEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPDFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(19630..20559)
                     /gene="kdgK"
                     /locus_tag="ON345_001027"
     CDS             complement(19630..20559)
                     /gene="kdgK"
                     /locus_tag="ON345_001027"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR3071125.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20791..21558
                     /gene="pdeH"
                     /locus_tag="ON345_001028"
     CDS             20791..21558
                     /gene="pdeH"
                     /locus_tag="ON345_001028"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR3071126.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLLALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21628..23688
                     /locus_tag="ON345_001029"
     CDS             21628..23688
                     /locus_tag="ON345_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR3071127.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRDFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23922..25244)
                     /locus_tag="ON345_001030"
     CDS             complement(23922..25244)
                     /locus_tag="ON345_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR3071128.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25655..26668)
                     /gene="yhjD"
                     /locus_tag="ON345_001031"
     CDS             complement(25655..26668)
                     /gene="yhjD"
                     /locus_tag="ON345_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR3071129.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26717..27616)
                     /locus_tag="ON345_001032"
     CDS             complement(26717..27616)
                     /locus_tag="ON345_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3071130.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            28136..28738
                     /locus_tag="ON345_001033"
     CDS             28136..28738
                     /locus_tag="ON345_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3071131.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28789..30438)
                     /locus_tag="ON345_001034"
     CDS             complement(28789..30438)
                     /locus_tag="ON345_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR3071132.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         7228 a         7784 c         8402 g         7133 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgcccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactata ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag acggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggtc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgatc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaata acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatccgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatactggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg caggcgggca ttttcaggtc tgcaaaatat tcgtggacgt
     5101 taccatcaat ggatgaacga gctgcctgag cgcgttagcc ataaaacaca gcatctggat
     5161 gagaaaaaag agctcggtca tttgagtgcc ggggcgcggc ggttgatcct cggtatcatc
     5221 gtcaccttct cgctgattct ggcgttaatc tgcgttactc aaccgtttaa cccgctggcg
     5281 cagtttatct tcctgatgct gctgtggggt gtagcgctga tcgtacggcg gatgccgggg
     5341 cgcttctcgg cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg ttatatctgg
     5401 tggcgttaca cctctacgct gaactgggac gatccggtca gcctggtgtg cgggcttatt
     5461 ctgctcttcg ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt ccaggtagta
     5521 tggccgctga atcgtcagcc ggtgccattg ccgaaagata tgtcgctgtg gccgtcggtg
     5581 gatatctttg tcccgactta caacgaagat ctcaacgtgg tgaaaaatac tatttacgcc
     5641 tcgctgggta tcgactggcc gaaagacaag ctgaacatct ggatcctcga tgacggcggc
     5701 agggaagagt ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc ccgcaccact
     5761 catgaacatg cgaaagcggg caacatcaac aatgcgctga aatatgccaa aggcgagttc
     5821 gtgtcgattt tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc
     5881 tggttcctga aagagaaaca gctggcgatg atgcagactc cacaccactt cttctcgccg
     5941 gacccgtttg aacgcaacct ggggcgtttc cgtaaaacac cgaacgaagg cacgctgttc
     6001 tatggtctgg tgcaggatgg caacgatatg tgggacgcca ctttcttctg cggttcctgt
     6061 gcggtgattc gccgtaagcc gctggatgaa attggcggca ttgctgtcga aactgtgact
     6121 gaagatgcgc atacttctct gcgtttgcac cgtcgtggct atacctccgc gtatatgcgt
     6181 attccgcagg cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt
     6241 cgctgggcgc gcgggatggt gcaaatcttc cgtctcgata acccgctcac cggtaaaggg
     6301 ctgaagtttg ctcagcggct gtgctacgtc aacgccatgt tccacttctt gtcgggcatt
     6361 ccacgactga tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc
     6421 tatgcgccag cgttgatgat cgccctgttc gtgctgccgc atatgatcca tgccagcctg
     6481 accaactcca agatccaggg caaatatcgc cactctttct ggagtgaaat ctacgaaacg
     6541 gtgctggcgt ggtatatcgc accaccgacg ctggtggcgc tgattaaccc gcacaaaggc
     6601 aaatttaacg tcaccgccaa aggtggactg gtggaagaag agtacgtcga ctgggtgatc
     6661 tcgcggccct acatcttcct tgtcctgctc aacctggtgg gcgttgcggt aggcatctgg
     6721 cgctacttct atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggta
     6781 ttctacaacc tcattgttct tggcggcgca gttgcggtat cggtagaaag caaacaggta
     6841 cgccgatcgc accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga agatggtcac
     6901 ctcttctcgt gtaccgttca ggatttctcc gacggtggtt tggggatcaa gatcaacggt
     6961 caggcgcaga ttctggaagg gcagaaagtg aatctattgc ttaaacgcgg tcagcaggaa
     7021 tacgtcttcc cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct gaaattaatg
     7081 ccgctcacca cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg tgcggataca
     7141 tgggcgctct ggcaggacag ctacccggaa gataagccgc tggaaagtct gctggatatt
     7201 ctgaagctcg gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc ttcggtgaag
     7261 ggcatattcc gtgtgctgac ttctctggtt tcctgggttg tatcgtttat tccgcgccgc
     7321 ccggagcgga gcgaaacggc acaaccatcg gatcaggctt tggctcaaca atgatgataa
     7381 cgcgatgaaa agaaaactat tctggatttg tgcagtggct atggggatga gtgcgttccc
     7441 ctctttcatg acgcaggcga cgccagcaac gcaaccactg atcaatgctg agccagctgt
     7501 agccgcccag acggaacaaa atccgcaggt ggggcaagtg atgccgggcg tgcagggtgc
     7561 tgatgcgcca gtcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga cctttgcgca
     7621 aattgcaccg ccgccgggca gcatggtgct acgtggcatt aacccgaacg gcagcattga
     7681 gtttggtatg cgcagcgatg aagtggtgac gaaggcgatg ctcaacctcg aatacacccc
     7741 atcgccatcg ttactgcctg tccagtcgca gttaaaggtt tatctcaatg atgaactgat
     7801 gggcgtgctg ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc aaatgcccat
     7861 taacccactg tttattaccg acttcaaccg tgtacggctg gagtttgtcg gccattatca
     7921 ggacgtgtgc gaaaacccgg ccagcaccac gctttggctg gatgttgggc ggagcagtgg
     7981 actggatctg acctatcaga ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc
     8041 attctttgac ccgcgcgata accgtactaa caccttgccg atggtctttg cgggtgcgcc
     8101 ggatgttggg ctgcaacaag cctctgccat tgtcgcctcg tggtttggtt cgcgttctgg
     8161 ctggcgtggg cagaacttcc cggtgctcta taaccaactg ccggatcgta atgccattgt
     8221 ctttgcaacc aacgacaaac ggccggactt cctgcgcgat catccggcgg taaaagcccc
     8281 ggtgattgag atgatcaacc atccgcagaa tccttacgtc aaactgctgg tggtgtttgg
     8341 tcgtgacgac aaagacctgt tgcaggcagc gaaaggtatc gctcagggta acattctgtt
     8401 ccgtggtgaa agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta agccgtacga
     8461 tgcgccgaac tgggtgcgta ccgatcgtcc ggtcaccttt ggtgaactga aaacctatga
     8521 agaacagtta caatccagcg gtcttgagcc agcagcgatt aatgtttcgc taaacctgcc
     8581 gccggatctc tacctgatgc gcagtaccgg cattgatatg gatattaatt accgctacac
     8641 catgccgccg gtgaaagaca gttcgcggat ggatatcagc ctgaataacc agttcctgca
     8701 atccttcaac ctgagcagca aacaggaggc gaaccgcctg ctgctgcgga ttccggtatt
     8761 acaaggtttg ctggatggca aaacagatgt ctctattccg gcgctgaaac tgggcgcgac
     8821 caaccagctg cgcttcgact ttgagtatat gaacccgatg ccgggcggtt cggtggataa
     8881 ctgtattacc ttccagccgg tgcagaatca tgtggtgatt ggtgacgact ccaccatcga
     8941 cttctcgaag tattaccact tcatcccgat gccggatcta cgcgcctttg ctaacgcggg
     9001 cttcccattc agccggatgg cggatctgtc gcaaaccatc accgtgatgc cgaaagcgcc
     9061 taacgaagca cagatggaaa cgttgctgaa tactgttggt tttatcggca cacagacggg
     9121 cttcccggcg attaatctga cggtgaccga tgatggcagc accattcagg gcaaagatgc
     9181 cgacatcatg atcatcggtg gtatcccgga caaactgaaa gacgataagc agatcgacct
     9241 attggtgcag gcgaccgaaa gctgggtgaa aacactgatg cgccagaccc cgttccccgg
     9301 cattgtgccg gacgagagcg atcgcgcggc agaaacccag tcaacgctga cctcttccgg
     9361 tgcgatggcg gcggtgattg gcttccagtc gccgtataac gaccagcgca gcgtgattgc
     9421 gctgctggca gatagcccac gcggttatga aatgcttaac gatgcggtga acgatagcgg
     9481 caaacgcgcc accatgttcg gttcggtcgc ggtgatccgc gagtccggta tcaacagcct
     9541 acgtgttggc gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct tgtggtatgc
     9601 gctggcaaac catccgattc tgctggcggt gctggcggct atcagtgtga tattgctggc
     9661 atgggtactg tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc cggataacga
     9721 gtaattgaag atgaatgtgt tgcgtagtgg aatcgtgacg atgctgctgc tggctgcctt
     9781 tagtgttcag gcagcctgta cctggcctgc ctgggagcag tttaaaaagg attacatcag
     9841 tcaggaaggg cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct ccgaagggca
     9901 aagttacggc atgttctttg ccctggcggc taacgaccgt gcagctttcg ataatattct
     9961 cgactggacg cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc ccgcctggct
    10021 gtggggcaag aaagagaaca gtaagtggga agtgctggac agcaattcgg cctccgatgg
    10081 tgatgtctgg atggcctggt cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta
    10141 taccgacatc ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc
    10201 tgggctgggt tccatgttgt taccgggcaa agtgggtttt gctgaggata acagctggcg
    10261 ttttaacccc agctacctgc cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc
    10321 gtggactacg ctgcgcgaaa ccaatcaacg tttattgctg gaaaccgccc cgaaaggctt
    10381 ttctccagac tgggtgcgtt atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac
    10441 attgatcagc agctacgacg ccatccgcgt ttacatgtgg gtaggcatga tgcctgacag
    10501 cgatccgcag aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat tcactgagaa
    10561 aaacggttat ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc
    10621 agtcggtttt tctgccgcca tgctgccctt tttacaaaac cgcgatgcgc aggccgttca
    10681 gcgccagcgc gtggccgata actttcccgg cagcgatgcc tattacaact atgtgctgac
    10741 cctgtttgga caaggctggg atcaacaccg tttccgcttc tcgacaaaag gtgagttatt
    10801 acctgactgg ggccaggaat gcgcaaattc acactaaaca tattcacgct ttccctcggt
    10861 ctggccgtca tgccgatggt cgaggcagca ccaaccgctc agcaacagtt gctggagcaa
    10921 gttcggttag gcgaagcgac ccatcgtgaa gatctggtgc aacagtcgtt atatcggctg
    10981 gaacttattg atccgaataa cccggacgtc gttgccgccc gtttccgttc tttgttacgt
    11041 cagggcgata ttgatggcgc gcaaaaacag ctcgatcggc tgtcgcagtt agcgccgagt
    11101 tcaaatgcgt ataaatcgtc gcggactacg atgctacttt ccacgccgga tggtcgtcag
    11161 gcactgcaac aggcacgatt gcaggcgacg accggtcatg cagaagaagc tgtggcgagt
    11221 tacaacaaac tgttcaacgg tgcgccgccg gaaggtgaca ttgctgtcga gtactggagt
    11281 acggtggcga aaattccggc tcgccgtggc gaagcgatta atcagttaaa acgcatcaat
    11341 gcggatgcac cgggcaatac gggcctgcaa aacaatctgg cgctattgct gtttagtagc
    11401 gatcgccgtg acgaaggttt tgccgtcctg gaacagatgg caaaatcgaa cgccgggcgc
    11461 gaaggggcct ctaaaatctg gtacgggcag attaaagaca tgcccgtcag tgatgccagt
    11521 gtgtcggcgc tgaaaaaata tctctcgatc tttagtgatg gcgatagcgt ggcggctgcg
    11581 caatcgcaac tggcagaaca gcaaaaacag ctggccgatc ctgctttccg cgctcgtgcg
    11641 caaggtttag cggcggtgga ctctggtatg gcgggtaaag ccattcccga actacaacag
    11701 gcggtgcggg cgaacccgaa agacagtgaa gctctggggg cgctgggcca ggcgtattct
    11761 cagaaaggcg atcgcgccaa tgcagtggcg aatctggaaa aagccctcgc actggacccg
    11821 cacagcagca acaacgacaa atggaacagt ctgctgaaag taaaccgcta ctggctggcg
    11881 atccagcagg gcgatgctgc gctgaaagcc aataatcctg accgggcaga acgcctgttc
    11941 cagcaggcgc gtaatgtcga taacaccgac agttatgcag tgctggggct gggcgatgtg
    12001 gcgatggcgc gaaaagatta tcccgccgcc gaacgttatt atcagcagac cttgcgtatg
    12061 gacagcggca acactaacgc cgtgcgcggg ctggcaaata tttaccgcca gcaatcgcca
    12121 gaaaaagctg aagcgtttat cgcctcgctc tctgccagtc agcggcgtag cattgatgat
    12181 atcgaacgca gcctgcaaaa cgaccgtctg gcacagcagg cagaggcact ggaaaaccag
    12241 ggcaaatggg cgcaggcggc agcacttcag cggcaacgac tggcgctgga ccccggcagc
    12301 gtatggatta cttaccgact ttcgcaggat ctctggcagg ccggacaacg cagccaggcc
    12361 gatacgttaa tgcgcaatct ggcgcagcag aagccgaacg acccggagca ggtttacgct
    12421 tacgggctgt acctctctgg tcatgaccag gacagagcgg cgctggcgca tatcaacagc
    12481 ctgccgcgtg cgcagtggaa cagcaatatt caggagctgg aaaccgctaa ccgcctgcga
    12541 gaaactaacc gcctgcgaga aagcggcaaa gaggcagaag cggaagcgat gctgcgccag
    12601 caaccacctt ccacgcgtat tgacctcacg ctggctgact gggcgcaaca acgacgtgat
    12661 tacaccgccg cccgtgctgc atatcagaat gtcctgacgc gggagccagc taacgccgac
    12721 gccattcttg gtctgacgga agtggatatt gctgccggtg acaaagcggc ggcacgtagc
    12781 cagctggcga aactgcccgc taccgataac gcctcgctga acacacagcg gcgcgtggcg
    12841 ctggcacagg cgcagcttgg cgataccgca gcagcgcagc ggacgtttaa taagttgatc
    12901 ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga tggtgctgcg cgacggtgcg
    12961 aagtttgaag cgcaggcggg cgatccaacg caggcgctgg aaacctacaa agacgccatg
    13021 gtcgcatccg gtgtgaccac gacgcgtccg caggataacg acacctttac ccgactgacc
    13081 cgtaacgacg agaaagatga ctggctgaaa cgcggcgtgc gcagcgatgc ggcggacctc
    13141 tatcgccagc aggatcttaa cgtcaccctc gagcatgatt actggggttc gagcggcacc
    13201 ggtggttact ccgatctgaa agcgcacact accatgttgc aggtggatgc gccgtattct
    13261 gacgggcgga tgttctttcg cagtgatttc gtcaatatga acgtcggcag tttctccact
    13321 aatgccgatg gtaaatggga tgataactgg ggcacctgta cattacagga ctgtagcggc
    13381 aaccgcagcc agtcggattc cggtgccagc gtggcggtcg gctggcgaaa tgacgtctgg
    13441 agctgggata tcggtaccac gccgatgggc ttcaacgtgg tggatgtggt cggcggcatc
    13501 agttacagcg atgatatcgg gccgctgggt tacaccgtta acgcccaccg tcggcccatc
    13561 tccagttctt tgctggcctt tggtgggcaa aaagactccc cgagcaatac cgggaaaaaa
    13621 tggggtggcg tacgtgccga cggtgtgggg ctaagtctga gctacgataa aggtgaagca
    13681 aacggcgtct gggcatcgct tagtggcgac cagttaaccg gtaaaaatgt cgaagataac
    13741 tggcgcgtgc gctggatgac gggctattac tataaggtca ttaaccagaa caatcgccgc
    13801 gtcacaatcg gcctgaacaa catgatctgg cattacgaca aagatctgag tggctactca
    13861 ctcggtcagg gcggttacta cagtccgcag gaatacctgt cgtttgccat accggtgatg
    13921 tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg cgtctggctc gtggtcgcat
    13981 tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc tgatcccgac cgactggcag
    14041 gaagaagctg cgcggcaatc caacgatggc ggcagcagtc agggcttcgg ctacacggcg
    14101 cgggcattac ttgaacgacg tgttacttcc aactggtttg ttggcacggc aattgatatc
    14161 cagcaggcga aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc
    14221 ggatggcagg gtgacatgga tttaccgccg cagccgctga taccttacgc cgactggtaa
    14281 gttttcagat agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg
    14341 ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca
    14401 atggtggcag ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat
    14461 ctggtccagc agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc
    14521 cgcgaaccct tatcttcagc tattttgaaa ggcgatattc ccgaagcgga agctattctt
    14581 gccagcatta aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc taaccagttc
    14641 caggcgctgc gtaaaagttt tattccagag cgcccggtgc cggtaatggt tactcgcctg
    14701 tttgagctac cggttcaaat ctcgctgggc gtttactcgc tcgaacgtcc ggcaaacccg
    14761 cagccaattg cctatctggt actacaggcg gattccttcc gtatgtataa gttcgtgatg
    14821 agcaccctct caacgttagt gaccatttac ttacttttgt cgcttatcct gaccgtcgcc
    14881 atcagctggt gcattaaccg cctgattttg catccgttac gcaatattgc tcgcgaactt
    14941 aacgccatcc cagcccagga gcttgttggt caccaactgg cattaccgcg tctgcatcag
    15001 gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc
    15061 cattatgaag aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa
    15121 gccttgctga tggagatgct ggagcaggtt gtcgcgcgta aacaaaccac cgcgctgatg
    15181 atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc tgaaagaggc gcaacgagaa
    15241 attctgctgc tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg tatgatcctc
    15301 gcgcagatta gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac
    15361 gcaatcacct taggtcagca agtgctcact atcatgagcg agcgcctgcc gattgaacgt
    15421 attcaactcc gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc
    15481 gaacagcttt acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaagaat
    15541 cagattcagt tctttgatcc gcagcagatg gaagccgccc agaagcggtt gacggaagag
    15601 agcgatatcc ttaatgcact ggaaaatcat caggttgcta tttggttaca gccacaggtc
    15661 gagatgacca gcggaaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat
    15721 ggcagttggg acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt
    15781 accgtcggtc attgggtgct ggaagagtcc tgtcgattgc ttgcagcctg gcaagagcgc
    15841 ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc aactgatgca cccgaatatg
    15901 gtggcggata tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg
    15961 gaagtgacag aaagccgacg tattgacgac cctcatgctg cggtggcaat cctccgtccg
    16021 ctgcgcaatg ccggagttcg ggtggcgcta gatgatttcg gcatgggcta cgcagggctg
    16081 cgtcagctgc agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt
    16141 gttgaaggct tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc
    16201 ctgaacttac aaatgattgc cgaaggcgtg gagactgaag cacaacgcga ctggctggca
    16261 aaagcgggcg ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc
    16321 ttcgaagaga gttacctgga agaaaagtag ctaccccaaa ctgattacaa aactttaaaa
    16381 agtgctggtt tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca
    16441 ggtacatttc tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc cataacctta
    16501 caagacctgt ggttttacta aaggacacct tatgaaaacc tctctgttta aaagacttta
    16561 ctttcaggtc ctgacagcga tagccattgg tattctcctt ggccatttct atcctgaaat
    16621 aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga tgatcatcgc
    16681 tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc atggaaagca tgaaggcggt
    16741 cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca tcgcgctgat
    16801 tattggtctt atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg tcgatccggc
    16861 aacgcttgat gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc agggcattgt
    16921 cgccttcatt atggatgtca tcccggcgag cgtcattggc gcatttgcca gcggtaacat
    16981 tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc tgggcagcaa
    17041 aggccaactg atttttaacg tcatcgaaag tttctcgcag gtcatcttcg gcatcatcaa
    17101 tatgatcatg cgtctggcgc ctattggtgc gttcggggca atggcgttta ccatcggtaa
    17161 atacggcgtc ggcacactgg tgcaactggg gcagctgatt atctgtttct acattacctg
    17221 tatcctgttt gtggtgctgg tattgggttc aatcgctaaa gcgactggtt tcagtatctt
    17281 caaatttatc cgctatatcc gtgaagaact gctgattgta ctggggactt catcttccga
    17341 gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta aatcggtggt
    17401 ggggctggtc atcccgacag gctactcgtt taaccttgat ggcacatcga tatacctgac
    17461 aatggcggcg gtgtttatcg cccaggccac taacagtcag atggatatcg tccaccaaat
    17521 cacgctgtta atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa cgggtagtgg
    17581 ctttatcgtg ctggcggcga cgctctctgc ggtgggccat ttgccggtag cgggtctggc
    17641 gctgatcctc ggtatcgacc gctttatgtc agaagctcgt gcgctgacta acctggtcgg
    17701 taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc acaaaaaact
    17761 ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg cacgaattat cctcttaatc
    17821 tcatcactta tgcccgcact cccttccccg agtgcgggtg tttgcgcata attgacccct
    17881 gtgcctgaac ttaccacctg ggtgagttca ggtttatttc acttcttccg tgacattttc
    17941 atgttcttgc ggtctaacac gaagtgtttt taacgtcatt tagactgacc gacaacgggc
    18001 agttttttat taccaggata gttgatcagg ggttcacatg cagggcacaa aaattcgact
    18061 tttagcgggc ggtttgctga tgatggccac tgctggctat gtgcaggcag atgcgctcca
    18121 gcctgatcca gcatggcaac aggggacgct ttccaacggt ttacagtggc aagtgctgac
    18181 caccccccag cgtcccagcg atcgtgttga aattcgcctg ctggttaata ccggttcgct
    18241 cgccgaaagt acacaacaga gcggttacag tcacgccatc cctcgtattg cgctaacgca
    18301 aagcggtggc cttgacgcag cacaggcgcg ttcattgtgg cagcagggga tcgaccctaa
    18361 acgcccgatg ccgccggtaa ttgtctctta tgacaccacg ctgtttaatc tgagtttgcc
    18421 caataaccgt aacgacttgc tgaaagaagc gctctcttat ctggcaaatg ccactggcaa
    18481 actgaccatc acaccagaaa ccatcaacca cgcgctgcaa agtcaggaca tggtggcaac
    18541 ctggcctgcc gatactaaag agggctggtg gcgctatcgt ctgaaaggat caaccttgtt
    18601 aggtcacgat cctgccgatc cgctgaaaca acccgttgaa gtggaaaaga ttaaagattt
    18661 ctatcagaaa tggtacaccc cggatgcaat gacgctgctg gtggtgggaa acgtggatgc
    18721 gcgctcggtt gtcgaccaaa tcaacaaaac gtttggcgaa ctgaaaggca aacgtgaaac
    18781 accggctccg gtgccgacgc tttccccgct gcgtgcggaa gcggtgagta ttatgactga
    18841 cgcggtgcgt caggaccggt tatctatcat gtgggatacg ccgtggcagc cgattcgtga
    18901 atcagccgca ctgctgcgct actggcgtgc ggacctggcc cgtgaggcgc tgttctggca
    18961 tgttcagcaa gcgttaagcg ccagtaacag caaagacatc ggtcttggat ttgactgccg
    19021 tgtgctgtat ctgcgtgcgc agtgtgccat caacatcgag tcaccaaacg acaagctgaa
    19081 cagcaacctt aacctggtgg cgcgtgaact ggcgaaggtt cgcgataaag gtctgccgga
    19141 agaagagttc aatgcgttag tggcgcaaaa gaaactggag ctgcagaaac tgtttgccgc
    19201 ctatgcacga gctgataccg atattctgat gggtcagcgg atgcgttcgt tgcaaaatca
    19261 ggtcgtcgat atcgcgccgg agcagtatca gaaactgcgg caggatttcc ttaacagcct
    19321 gacggtggag atgttaaatc aggatctgcg tcagcagttg tcgaatgata tggcgttaat
    19381 actgctgcag ccgaaaggcg agccggattt taacatgaaa gcgttgcagg cggcctggga
    19441 tcaaatcatg gccccatcta ccgccgctgc gaccacctct gtcgccacgg atgacgtaca
    19501 tcctgaagtg acggatattc cacctgcaca gtaatgcctg atggtgatga gttagcacaa
    19561 ccatcaggta tttatggatg agctggatag aggggtaaca ctttatccag ccttttgcat
    19621 atgctgcttt tacgctggca tcgcctcacg cgggataatc gcgccgcgat actgaataac
    19681 ggtacttgcg gtcaggtgcc cgcgtttcgc tgcgttttcc gcactgccgc ccgtcaaacg
    19741 taccgccaga taaccggcac tgaaagagtc gccagctgcg gtggtatcga tcactttttc
    19801 tttcggcagt ttcaccgccg gaacatccac taacccttcg ccagcaatgg acaccaggca
    19861 agaatccgcc ccgcgtttca ccaccacttc tttcacgccc gcgttatggg tgcgcgcaat
    19921 gacgtcttcc accggttgtt gaccccacag cgcgtcttca tcgtccagcg tcaggaaggc
    19981 gatatccgtg cattccagca tttgttggta cacctgctgt gtctcttctt tgctggccca
    20041 caggcgcgga cgatagttat tgtcgaaaat cacttttccg ccgttggcgc ggcattcgcg
    20101 cagcagggaa agcagctttt cgcggctggt cgggcttaag atcgcaaggc taatcccgct
    20161 caggtagaga taatcgaaat tcgccagctc ttcgcaaatc gccgcagact gctcactctc
    20221 cagccagaat ttggcggcgg cttcgttccg ccagtagtag aacgtacgtt cgccggtgct
    20281 gtcggtttca atgtagtaaa ggcccggaag acggttttcc atccgttggg tcagggaagt
    20341 atcaacgttc tcgccgtgcc aggcgtccag catctgctgg ctaaaactgt ccgttcccag
    20401 cgccgttacg taatgaacgg ttaatgccgc aggatcgacc tgacgggcga tatagacgga
    20461 agtgttcagg gtatcgccgc cgaaaccgcg cttaacgtcc gcgcctttct cggaaagctc
    20521 aatcatgcat tcgccaatca cggcaatctt tttggacata gtcgtgaacc tgatctgtaa
    20581 aaaattagcg ttagtttgcg ctgtggtggt ttgctggtca accatattaa aacagtgttc
    20641 cattattttt ttgagccaga acgtattcct gaaagattcc gttgtggagt gaggaaattt
    20701 accccggcca ggcggtaaag ttctgccctt acgcgccgat aatctttgtc gagtccgggc
    20761 agcatcactt ttaaacacag gacatctttg atgataaggc aggttatcca gcgaataagc
    20821 aaccctgaag caagcatcga gagcttgcag gaacggcgtt tttggttgca gtgtgagcgt
    20881 gcttacacct ggcagccgat ctatcaaaca tgcggacggt taatggccgt ggagctatta
    20941 acggtggtca cgcatccctt gaacccttcg caacgcctgc cgccggatcg ctattttact
    21001 gaaatcaccg tcagccatcg gatggaggtt gtgaaagagc agattgattt gctggcgcaa
    21061 aaagccgact tctttgtaga gcacgacctg ctggcatcgg tcaatatcga tggccctacg
    21121 ctcctcgccc tgcgtcagca accaaaaatc ctgcgccaga ttgagcgtct tccctggctg
    21181 cgtttcgaac tggtggagca tatccgtctg ccgaaagatt caacctttgc ctcgatgtgt
    21241 gaatttggcc cgctgtggct ggatgatttt ggtaccggga tggcaaattt ctctgcacta
    21301 agtgaagtgc gttatgacta catcaaaatc gcgcgagaac tgtttgtgat gctgcgtcag
    21361 tcgccggaag gccgcacact cttttctcag cttttacatc taatgaatcg ctattgtcgc
    21421 ggggtgattg tcgagggcgt agaaacgccg gaagagtggc gtgatgttca gaactcgccc
    21481 gcattcgccg cacaaggctg gtttctttca cgcccggcac cgatagaaac gctgaatacg
    21541 gcggttctgg cgctataagc tgcctcattt tccgcctggc tggactatct ttaggactgg
    21601 cacaggaaga atatgaggaa gcgaacgatg agcaaggcag gcaaaataac cgctgcgatt
    21661 tcaggggctt tcttgttgtt gattgtcgtg gcgatcattt tgattgcaac ttttgactgg
    21721 aatcgactca aaccgaccat caaccagaaa gtctctgcgg agttgaatcg tccgttcgct
    21781 atccgtggcg atctgggcgt ggtgtgggag cggcaaaaac aagaaactgg ctggcgcagc
    21841 tgggtgccgt ggcctcatgt acatgcagaa gacatcattc ttggtaatcc accggatatt
    21901 cccgaagtca cgatggtgca tttgccacgc gtagaggcaa cgctggcccc gctggcgctg
    21961 ctgaccaaaa cggtctggct gccgtggatc aagctcgaaa agcccgacgc gcgcctgatt
    22021 cgcctctccg aaaagaacaa taactggacg tttaatcttg ccaacgatga caacaaagac
    22081 gcgaatgcaa agccgtcggc atggtcgttt cggctggata atattctttt cgatcaaggg
    22141 cggatcgcca ttgatgacaa agtaagcaaa gcggatctgg aaatttttgt tgatccgtta
    22201 ggcaagccgc tgccattcag cgaagttact ggatcgaaag gtaaagcgga taaagaaaag
    22261 gtgggcgatt acgtttttgg cctgaaggcg cagggacgtt ataacggtga accgctcacg
    22321 ggtacgggaa aaataggcgg tatgctggcg ctgcgtggcg aagggacgcc gtttccggta
    22381 caggctgatt tccgttcagg taatacccgt gttgcttttg atggcgtcgt gaatgaccca
    22441 atgaagatgg gcggtgtcga tttacggctt aaattttctg gcgattcact gggtgatctc
    22501 tatgaactga cgggcgttct gctgcccgat accccgccgt ttgaaacgga tggtcggctg
    22561 gtagcgaaaa tcgacactga aaaatcgtcg gtctttgatt atcgcgattt taatgggcga
    22621 attggcgata gcgatatcca cggttctctg gtctacacca ccggaaagcc acgaccaaaa
    22681 ctggaaggtg atgtcgagtc gcggcaattg cggctggcgg acctgggacc gttgattggc
    22741 gttgattccg ggaaaggggc agaaaagtcg aaacggtctg aacagaagaa gggcgaaaaa
    22801 agcgttcagc ctgcgggcaa agtgctgcct tatgaccgct tcgaaaccga taaatgggac
    22861 gttatggatg ccgatgttcg cttcaaaggg cggcgcattg agcatggcag tagcctgccg
    22921 attagcgatc tttctactca tatcatcctc aaaaatgctg acctgcgcct gcaaccgctg
    22981 aaatttggca tggcgggcgg cagcattgcg gcgaatattc atctggaagg cgataaaaag
    23041 ccgatgcagg ggcgggcaga tattcaggct cgtcgactga aactgaaaga actgatgccc
    23101 gatgtggaac tgatgcagaa gacgctgggg gaaatgaacg gtgacgcgga actacgcggt
    23161 agcggtaact cggtggcggc gcttttaggc aacagtaacg gcaacctgaa actgttgatg
    23221 aatgacgggc tggtgagccg caacctgatg gagattgttg ggctgaatgt cggcaactac
    23281 attgtcggtg cgatatttgg tgacgatgag gtgcgggtga actgcgcggc ggcgaatctg
    23341 aatattgcca acggcgtggc gcgcccgcag atttttgctt tcgatactga gaacgcgttg
    23401 attaacgtta ccggcacggc aagttttgct tccgaacagc tggatttgac tattgatccg
    23461 gagagtaaag gaattcggat tatcacactg cgttcgccgc tgtatgtgcg tgggacgttt
    23521 aaaaatccgc aggctggggt gaaagccggg ccgttgattg cccgtggtgc tgttgctgcg
    23581 gcactggcaa cgctggtaac accggcggcg gcgttactgg cactgatctc accttccgaa
    23641 ggggaggcta atcagtgtcg gacgatattg tcgcagatga agaagtgatg aggttggatt
    23701 gtgtttgctg gtgagttcgg gagcagcgtg ttcccctcac cctaaccctc tccccaaagg
    23761 ggcgagggga ctgttttgtg cgtgttgaaa caacgagtac cgcttttccg ttcactttct
    23821 gggggagggc tgttatgttc gcgatataac atctcgctcc gcttttctcc ctctcccttt
    23881 cagggagagg gctggggtga gggtggctgc ccacacgata cttacaacga ctgatgtcgc
    23941 gtctcatggg tcagcagcag ggcgattaac gtcaagccag ccatcgccgc cagatataac
    24001 cccaccgcac ctagcccgta gttagtctgc aaccaggctg cgatatatgg cgcaacggaa
    24061 gccccgagaa tcgacgctac gttgtaagag aacgatgctc cggtgtaacg cacttctgtc
    24121 ggaaacagct ctggtaacag cgcacccatt ggcccgaagg tcagacccat cagacttaac
    24181 cccagcagca ggaaggcaaa aaccagaatc gggttgccag aaccgagcag tgggttaaag
    24241 gcgaacagcg cgaacaggat gatcagcgtg gtgatgatta ccatgctttt acggcgacca
    24301 aaggcatcag ccagtaatcc agcgactggc accatcacgc caaaaccaat aactgccatc
    24361 atcaacatcc acaacacttc gttacgcggc aggccaagcc caactggcgc ggcggcggta
    24421 ctaaaggtca tagagtagac cgtcatgatg taaaacagcg tataggttgc cagcataatg
    24481 aacgtaccca gtacggttac gcgaacatgt ttggtcagca gcgtacccag cgggatcttc
    24541 acctgttttt tcgctttagc gactttctca aacaccggcg actcatgcag cgacacgcga
    24601 acatacaggc cgataatgac cagcaccgcc gagaagataa atggcacacg ccagccccag
    24661 ctcataaact gctcgtcggt cagtagccag gaaagcagca agaaagtgcc gttggcaaag
    24721 aagaagccaa tcggtgcgcc cagctgcggg aaggagccgt acaatgcacg tttgcgcggt
    24781 ggggcgtttt cagtcgccag cagcgccgcg ccgccccatt caccacctaa gcccagaccc
    24841 tgaccaaatc gagccagcgc cagcagcagc ggggcgaaaa taccaatcgt ggcatagcct
    24901 ggcagcagac caatcaccac ggtcgaaatc cccatcgtta gcaacgaggc gaccagcgtc
    24961 gctttacgcc caacgcgatc gccaaaatga ccaaaaacgg cagagccaat ggggcgcgcg
    25021 acgaaggcga tggcgaaggt ggcgagcgac tgtagcgttg ctgccgttgg atcgccctgc
    25081 gggaagaaga tatgcggaaa cacaataacg gccgcagtgg cgtaaatgta aaagtcgaag
    25141 aactcaatgg ccgtgccaat gagagaggcg acaaggactt tattacgcga gttgatcggc
    25201 gtgtattctt gctcgtggtc gagtgttgtg gctgttgctt gcataatctt ttcttatttt
    25261 ttggctaacg aatagccata ttacgcacag caaaagtgat atttcaatgt gtaacaaatc
    25321 gcggaaatgg tcaaaaacca ggcaaaaagg ggataatgtt taggccagtg atttttcttc
    25381 tatgaaatgt ttcacataat tttataaata gtggataaaa ctggtttcag gttaaataaa
    25441 agttaccaac tggatttata tgctggggtt atgagttgga atgaaattgt ttgctcccaa
    25501 atagatggct gaagaggctc tggggaagag tttcaagagg ttatgtgcgc attatagccg
    25561 gatgcgatgc tggcgcatct tatccggcct acggggagcc aggccgaata aaatcgtatg
    25621 ccggataagg cgtttacgcc gcatccggcc tattttaagg ctgcgttttc cctggcattc
    25681 gcgggtcgtc tttatattcg gcggtggcaa tccacgccgc gcaaaacagc gtcaaacggg
    25741 cgaagaagta gaaaaatgcc atcagcccca gcacggaacc aaatgctgcg ccagaggggg
    25801 atttcatcag cgatggcagg gtgtaggtca tcacgatttt aatcacttca aaaccaatag
    25861 cagccagaaa tgttccgcga atcagggctt ttttgcgcgg acggtggcgc ggcaaccgcc
    25921 agaagatcca gaagaaaagc agatagttgg cgaagatgga aatcgccagg ccaatcaatc
    25981 gccacgtcgg tttcagccac tcaatgctgt tcaggtgcag ggcgctaata atcatttgct
    26041 gcgccgaacc ggcaaccgag gtgatcgaaa gcgtcacaat cagcgcaatc aacaaaccaa
    26101 tcagtgaaat aaaatcacgc agatatttta cccagaactt ctcctgatct tgcggcgagc
    26161 gttcccagac atcgcgcgac tgggcacgaa tcgcttcacg caggttaccc atccagttga
    26221 tgccggaata aagtgccacc gccaggccga caagccctac agtcgtacgc tgctgaacgg
    26281 cggtgttgat ggtgtttttc aacgtggcgg ctagtgtcgg atcgctgatg ttttgcagaa
    26341 ttttgtcgaa gatatcctgt aacaacatcg gatgggaggc cagcacaaag ccccctgcgg
    26401 caaacgacac catcaaaatc gggatcatcg acaaaaatga gaaataggtg atagccgcac
    26461 caaactggtt acccagccga tcgttaaagc gttctgtcgc gcgaataagg tgcgcaataa
    26521 ccggttggcg ttggactttt tcggcagtgg tggtgacggt ttccagcgcc tggctaactt
    26581 tgccgccttt ctcgctatta tccaacggct taatcggctc gtgctccaga tcctgggtgg
    26641 gacgtttgat ctcgttttcc tgcgtcatcc gctcttttgt ccttctgtcg tggtcattct
    26701 taaaaagtat agtcggtcag tccaggtgct ctttcattac gccagccagc cactgcataa
    26761 acaggtttac acgccgggaa agctcccgac gctgcggata aaccagcgaa agggagagcg
    26821 gctcggcacg gtagcctggc aacacttcaa taagccgccc ggcacgcagg gcttcgcgca
    26881 cggcaatgcg cgggatctga ataatcccca gaccggcaag accggcggcg agatagtttt
    26941 cgctactgtt taccgtcaac atgccgccag acttaaacca ctggacgcca ttaacgctgg
    27001 caacctcaaa acctaacgga tgtacaccca ggtgcggtgt ataacgcact attgcgtgtg
    27061 aagtcagatc atcggggctt tgcggataac caaagcgcgt cagatagtgc ggactggcac
    27121 agttgaccat ggtcagtttg ccgaggggac gggcgataac gccatcttcc ggtaacgcgc
    27181 cagtgcgtat cacgcaatca aaaccatcat gaagaatatc taccggacgg tcatggctac
    27241 tcagttccag ctcaattccc ggatgcagat agagaaattc cgacaggcgc ggcagtaaca
    27301 ggcttttcgc gattcctggc gggatgtcga tgcgtaattt accgctgata ctggtggcat
    27361 cctgttgaaa cagaccgtcc agttcgctga gattactcaa cacatctttt gctcgttgat
    27421 aataggtcat gccttctggc gtgagtttga cccgtcgcgt ggtgcgctgg agaagctggg
    27481 tgccaagttg atgttccagt gcctgtatct ggcgcgaaac acttcctttt ggcaaagcaa
    27541 agaaatccgc cgcgcgggaa aaactttcca gctccgcgac tttgatgaac aactgcattg
    27601 cgtgaatttt atccatttct ttgaccgatt gttgttcaca gcgaaacagt aacgcgcata
    27661 tcgcattgtt tattgttttt tctaacatct aatgccgggt ttcacaatgt tgtcatgacg
    27721 aatgtggcga tttctcagca ggaaaagggc ggaatagctt tctgagaaag gtgaatcccc
    27781 ctaaacagag cgtatttttc agcgattcct tttaaagtga atagaggacg tattggctat
    27841 ttgtgcttat ttttgtctca ttgccgtttg ttattttggc ttgtggtcgc ggaatttcat
    27901 ttttaattgc atgcgtacta accatcaata taaggaaatc ctcaaaattg ttttaaatag
    27961 tgccctcgac atgtaatttc aggcgtttag ctgtaactat ttatatcggt aactattttc
    28021 tacgcgtcat ctctgtagaa tggccaccgc caaatggcga attcttattt tttctcgcgg
    28081 gtaataccct tccactactt aagtcttttt aacgtattca ggtggagatt cagttatgca
    28141 aatagtcatg tttgacaggc agtcaatatt tattcatgga atgaaaatca gtttacagca
    28201 gcgtattcca ggagtgagta ttcagggggc cagtcaggca gacgagttat ggcaaaagct
    28261 ggaaagttac cctgaagcct tagttatgct cgatggcgat caggatggtg agttttgcta
    28321 ctggttgctg caaaaaaccg tggtgcagtt tcctgaggtt aaggtgttaa tcacggcgac
    28381 ggattgcaat aaacggtggt tacaggaagt tattcatttt aatgtgctgg ccattgtgcc
    28441 tcgtgattca accgtcgaga catttgcgtt ggcggtaaat agtgcggcga tggggatgat
    28501 gtttctgcct ggagactggc ggacgacgcc ggaaaaggac attaaagatc tcaaatcgtt
    28561 aagcgcccgc cagcgggaga ttttaaccat gttagcggcg ggcgaatcaa ataaggagat
    28621 cggcagagcg ctgaatatca gtaccggaac ggtaaaagcg catctggaat ccctgtatcg
    28681 ccgtctggaa gtgaaaaatc gcacccaggc ggcaatgatg ttaaatatct cctcctgaag
    28741 aggataattt tacagccagc aggaagttag cggctggctg taaaaatatt atggttcgcc
    28801 gtacaaacca attaaacggc gtaccacacc gttagtccag ccaaacccat cctgcaacgg
    28861 atactcgcca ccgccgcctt cgcggggaac accatcggca atatggtatt tctcgatcat
    28921 tttgtgctgt tccagataga actgattcac cgttttcagc cagctgcgcg cgatttcatc
    28981 acccagaagg tcatcgccat acattttaaa tccctgaatt gccatccatt gtaacggtgc
    29041 ccagccattg ggtttatccc actgttcacc ggtttcgtac tcgcttgcca gaatcccgcc
    29101 aggtgtcagt aaccggctgc gtacggcgtt tgccagacga tcggcctgtt catggttcgc
    29161 catgccgaca tagagcggca caatggcggc agcggaaaac agcgccagtt gttcgcgtcg
    29221 ccagtcgtaa tcgcggtaga tgccgttttc atcatcccag aggtaacggt ttaccgcatc
    29281 gcgacgggca ctggccttct ggcggaacag cgcttctgtc tctttctcgc ctttcagcgc
    29341 cgagatgttg gcgatggcgc tctccagttt aaacaggaag gcattcagat cgatggggat
    29401 gaactgggtg gtacgaatgc tcgccagacg accagtatca cgcagccaac gggaagagta
    29461 atcccaacct gaggccgctc ccgcgcgtaa atcgcggtac acctcgttgg gcgggcgacc
    29521 agaatgtttc gcggtttcaa cgtcctcaag ccaggattcg tcacgcggcg tgtcgcgatc
    29581 gtcccagtaa cggttgagca gcgatccgtc cggcatccgc acaacatggc gataggcctg
    29641 attagggatc aacgattctg caccgtccat ccagaaggca tattccattt taaggtggtc
    29701 gagatagcgg cgcgcaccgc gtacaccatc ttcttcaaac aactccacca tcagcgcaaa
    29761 aaccggtggt tgcgagcggc tcaaataata ggtgcggttg ccgttgggga tgtgaccgta
    29821 gttttcgatc atccaggcga agttatcggc catgcatttc agcaaatctt cccgaccact
    29881 ttccgccagc cccagcatgg tgaaatagga atcccaatag taggtttcgc taaaacggcc
    29941 gcccgggaca atatatgact gcggtagcgc cagcagagaa gaccacggaa tgtgatcctg
    30001 tggttcgcgg gttagcaccg gccacagctg gtcgatatgc tccttcaggg aattttgcgg
    30061 gtccgataca tactcgctgg agtagacctc cggcagccag aagtgatttt caacaaactt
    30121 gcgcaagtca aaatcacgat gacggcgcac tttacggtag cggattaaaa tatccagcgg
    30181 gtccattttg ggtgcgcagt cgggaaaggt tttactgtcg gggaaaattt tcgccgactg
    30241 aacatgctcg aacagttcga gatagcgatc ggcaggcgtc agcgcatcag aggcgggcag
    30301 cccttcaatc atctcgggtt ccggttctgc ctcgatcatc tcatccagtt ttaattcata
    30361 cgggtccagt tcatagcaga gatcgacttc gatcatcagt tcgtctggat tagggttttg
    30421 aattttctga ttgagcataa ggatccaacc tccgaaattc gtcgctaatt atcgggcttt
    30481 tctttaaggg tagtcattcg ctccgcctgg tgggcgacaa agcgtgcggt ggatcacgtt
    30541 tattaac
//
LOCUS       DAJSKJ010000026        29279 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000026 DAJSKJ010000000
VERSION     DAJSKJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.026"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..479)
                     /gene="sulA"
                     /locus_tag="ON345_001035"
     CDS             complement(<1..479)
                     /gene="sulA"
                     /locus_tag="ON345_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR3071133.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     "
     gene            698..1327
                     /gene="sxy"
                     /locus_tag="ON345_001036"
     CDS             698..1327
                     /gene="sxy"
                     /locus_tag="ON345_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR3071134.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1290..3452)
                     /gene="yccS"
                     /locus_tag="ON345_001037"
     CDS             complement(1290..3452)
                     /gene="yccS"
                     /locus_tag="ON345_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR3071135.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAIWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDQSQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3462..3908)
                     /locus_tag="ON345_001038"
     CDS             complement(3462..3908)
                     /locus_tag="ON345_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR3071136.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4031..6085
                     /gene="helD"
                     /locus_tag="ON345_001039"
     CDS             4031..6085
                     /gene="helD"
                     /locus_tag="ON345_001039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR3071137.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLQDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6117..6575)
                     /gene="mgsA"
                     /locus_tag="ON345_001040"
     CDS             complement(6117..6575)
                     /gene="mgsA"
                     /locus_tag="ON345_001040"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR3071138.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6671..7333)
                     /locus_tag="ON345_001041"
     CDS             complement(6671..7333)
                     /locus_tag="ON345_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR3071139.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7506..7919
                     /locus_tag="ON345_001042"
     CDS             7506..7919
                     /locus_tag="ON345_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR3071140.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7964..8281)
                     /gene="hspQ"
                     /locus_tag="ON345_001043"
     CDS             complement(7964..8281)
                     /gene="hspQ"
                     /locus_tag="ON345_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR3071141.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8339..9529)
                     /gene="rlmI"
                     /locus_tag="ON345_001044"
     CDS             complement(8339..9529)
                     /gene="rlmI"
                     /locus_tag="ON345_001044"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR3071142.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9624..9902
                     /gene="yccX"
                     /locus_tag="ON345_001045"
     CDS             9624..9902
                     /gene="yccX"
                     /locus_tag="ON345_001045"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR3071143.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIH"
     gene            complement(9899..10228)
                     /gene="tusE"
                     /locus_tag="ON345_001046"
     CDS             complement(9899..10228)
                     /gene="tusE"
                     /locus_tag="ON345_001046"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR3071144.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10319..10978)
                     /gene="yccA"
                     /locus_tag="ON345_001047"
     CDS             complement(10319..10978)
                     /gene="yccA"
                     /locus_tag="ON345_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR3071145.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11185..11272)
                     /locus_tag="ON345_001048"
     tRNA            complement(11185..11272)
                     /locus_tag="ON345_001048"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11236..11238),aa:Ser,seq:tga)
     gene            11699..12817
                     /gene="hyaA"
                     /locus_tag="ON345_001049"
     CDS             11699..12817
                     /gene="hyaA"
                     /locus_tag="ON345_001049"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR3071146.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIMQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12814..14607
                     /gene="hyaB"
                     /locus_tag="ON345_001050"
     CDS             12814..14607
                     /gene="hyaB"
                     /locus_tag="ON345_001050"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR3071147.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LSVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            14626..15333
                     /gene="hyaC"
                     /locus_tag="ON345_001051"
     CDS             14626..15333
                     /gene="hyaC"
                     /locus_tag="ON345_001051"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR3071148.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15330..15917
                     /locus_tag="ON345_001052"
     CDS             15330..15917
                     /locus_tag="ON345_001052"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR3071149.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDHGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            15914..16312
                     /gene="hyaE"
                     /locus_tag="ON345_001053"
     CDS             15914..16312
                     /gene="hyaE"
                     /locus_tag="ON345_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR3071150.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16309..17166
                     /locus_tag="ON345_001054"
     CDS             16309..17166
                     /locus_tag="ON345_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR3071151.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            17300..18844
                     /gene="appC"
                     /locus_tag="ON345_001055"
     CDS             17300..18844
                     /gene="appC"
                     /locus_tag="ON345_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR3071152.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAMMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRKRDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18856..19992
                     /gene="appB"
                     /locus_tag="ON345_001056"
     CDS             18856..19992
                     /gene="appB"
                     /locus_tag="ON345_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR3071153.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTLQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLV
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            20005..20097
                     /gene="cbdX"
                     /locus_tag="ON345_001057"
     CDS             20005..20097
                     /gene="cbdX"
                     /locus_tag="ON345_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR3071154.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            20177..21475
                     /gene="appA"
                     /locus_tag="ON345_001058"
     CDS             20177..21475
                     /gene="appA"
                     /locus_tag="ON345_001058"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR3071155.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(21590..23770)
                     /gene="etk"
                     /locus_tag="ON345_001059"
     CDS             complement(21590..23770)
                     /gene="etk"
                     /locus_tag="ON345_001059"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR3071156.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(23790..24236)
                     /gene="etp"
                     /locus_tag="ON345_001060"
                     /gene_synonym="yccY"
     CDS             complement(23790..24236)
                     /gene="etp"
                     /locus_tag="ON345_001060"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR3071157.1"
                     /translation="MAQLKFNSILVVYTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(24224..25363)
                     /locus_tag="ON345_001061"
     CDS             complement(24224..25363)
                     /locus_tag="ON345_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR3071158.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKACVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(25409..27505)
                     /locus_tag="ON345_001062"
     CDS             complement(25409..27505)
                     /locus_tag="ON345_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR3071159.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDACWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(27505..28251)
                     /locus_tag="ON345_001063"
     CDS             complement(27505..28251)
                     /locus_tag="ON345_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR3071160.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(28248..28892)
                     /gene="gfcB"
                     /locus_tag="ON345_001064"
     CDS             complement(28248..28892)
                     /gene="gfcB"
                     /locus_tag="ON345_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR3071161.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(28999..29277)
                     /locus_tag="ON345_001065"
     CDS             complement(28999..29277)
                     /locus_tag="ON345_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071162.1"
                     /translation="MAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTGATTTATTTG
                     GVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         7068 a         7468 c         7734 g         7009 t
ORIGIN      
        1 ccggaaagtt gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata
       61 gcgttacctt catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct
      121 gccaaccaac cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc
      181 acagtgtggc aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat
      241 gcctgaaccc attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat
      301 tgctgaccga gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct
      361 tcgcgataga caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca
      421 attttacttg ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata
      481 atcaatccag cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga
      541 tcaaccctat tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa
      601 ttaatcttgc cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg
      661 aatcattctt ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg
      721 gatctataaa tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt
      781 tggcagttac agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt
      841 gtatcttcgg gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct
      901 gacatataaa aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct
      961 atggcgaaat caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa
     1021 agagaaaagc acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca
     1081 tctggaagcg attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg
     1141 ggcaaaaatg tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct
     1201 gtttatgctt gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg
     1261 ccaggagctt gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact
     1321 tgagtaatct ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact
     1381 tgttgtacga ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt
     1441 ttcaggctag ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg
     1501 tcatcaacat agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc
     1561 tcccggtgag caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac
     1621 cgaaacgcgg cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc
     1681 accgacgcca gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga
     1741 cggttatcac gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc
     1801 tctgtggcgc gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag
     1861 ctcactgccg cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct
     1921 acttcaaaac cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc
     1981 gttgcatgag cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc
     2041 agcaccagct gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata
     2101 cctaccagcg taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc
     2161 tggcagacaa acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga
     2221 atgatggcgt agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg
     2281 gattccggcg tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg
     2341 tcatcagcga gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca
     2401 attgttgcca gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg
     2461 agtaaatcgg cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta
     2521 aaagcgcgct caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac
     2581 agttgctggc acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg
     2641 ctgtggcgaa aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg
     2701 tgaatatcct gtgcgacaaa gtaataatgc agcgtgcggc gcgttccccg ttgaccacga
     2761 tcgccacgta agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga
     2821 ccgttggcga gagccaaatc gtacagcggt gcctggcttt gatcttcaat atcaggatca
     2881 aacatgcgcg acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg
     2941 tcctgcagcg ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag
     3001 atggcaccgg ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc
     3061 aacatagtgt aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc
     3121 agaccgccga gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag
     3181 ggccagggaa acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg
     3241 atgaggttac gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc
     3301 atccccagcg ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg
     3361 gttccacaaa gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag
     3421 cgtttgagca aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt
     3481 gcattggctt cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc
     3541 catagtgcaa tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa
     3601 atacattgtg cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc
     3661 agccagaaaa tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg
     3721 ttcagttcat cgacatgaat agcttcattg ccataaggca ccagagacag tttagtgatc
     3781 tcccagcagg atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc
     3841 aacagccagc ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg
     3901 gtacgcataa aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt
     3961 tttgggctgg agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat
     4021 ctacagagtc atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta
     4081 cgatcgggcg gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct
     4141 tattcctttc aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct
     4201 ggaatttgta ctgcaggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac
     4261 gcagcgtttt taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga
     4321 aattgcgtct ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa
     4381 taaatggctg acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc
     4441 ggcgttgccg ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg
     4501 taaatgtcag gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta
     4561 taccgaagcc atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct
     4621 gaatccggcg caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg
     4681 tgcaggaagc ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg
     4741 tgaagcgtcc cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat
     4801 ggacgagcgg attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc
     4861 gctggcgctg catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga
     4921 aaatgatacc gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga
     4981 aaagaaagcg caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt
     5041 gccagaaggt aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga
     5101 tcgctgggta agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc
     5161 acccgaagag attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa
     5221 agcctggaaa ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca
     5281 ggcgattgtg attctggaga aaggtcgctt tattagcccg tggaagcata ttctggttga
     5341 tgaatttcag gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa
     5401 cagtcagacg acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg
     5461 tgcgcaaatg tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga
     5521 tttagacacg acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc ggtttattca
     5581 gcagaaccca ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc
     5641 cgtcacgtta ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc
     5701 caaaccggaa gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct
     5761 ggaaaaagcg gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag
     5821 caaagggcaa caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt
     5881 tccggctgcg gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt
     5941 cccggacgct gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt
     6001 atgggcactg tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga
     6061 tgtgccggtg gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac
     6121 ttcagacggt ccgcgagata acgctgataa tcggggatca gaatatcgac cgcgtcgttg
     6181 aaatgcggcg actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc
     6241 cataccgtcg ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt
     6301 ggatcccaga agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc
     6361 tggtcacccc ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa
     6421 attaagttac cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc
     6481 cgttccaccc agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt
     6541 ttccgcgcag gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc
     6601 tacagattac tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg
     6661 cacaaaaatc tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt
     6721 gtgggtcagc cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg
     6781 gtggaatagc atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg
     6841 tggcaaattg cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct
     6901 cataatcaat cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg
     6961 gcgtcgcatc gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc
     7021 gctcattctc caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac
     7081 tgaccaccag cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct
     7141 tctcaacgcg aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc
     7201 gcagcagaga gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa
     7261 gccgcagcgt ggtggcaaaa acggataccg gcaaacataa tgcaatcaag gtggtcacga
     7321 tgccggtttt cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt
     7381 tttctttaat catacttact aacatataga catatttccc gcttttgctc tcattcattc
     7441 gtattagctg catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg
     7501 aaataatgaa agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg
     7561 tgggggcaag cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc
     7621 aggggtatca cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc
     7681 aggggtatgg cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta
     7741 attcagaagc ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct
     7801 ggatgcaact gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga
     7861 atgtggtgat ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa
     7921 aaatccccgg aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg
     7981 gggcctggag ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat
     8041 gttcatcctg caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc
     8101 cgttatcgtc ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg
     8161 ccagctcatc aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc
     8221 cgagataacc taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca
     8281 tagtcacctc ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt
     8341 acatgacgcg acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg
     8401 gatgatcggc tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg
     8461 cggcatccgc gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag
     8521 tcaggagaat accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc
     8581 cacgacacgc gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga
     8641 taacgtcaaa tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac
     8701 ggacaaactc agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat
     8761 ccagcgcttc ctgggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg
     8821 atacggcgaa accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc
     8881 ggcgggtagc caggcggcta tcacgctggt ccaggtagta gcccgttttg tgtccgtgct
     8941 gaatatccac cagcagtttc atgccgtgtt cttcaatcgg cagcagggca ggtggcaact
     9001 cgccggtgac gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc
     9061 tgcgatcgta aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc
     9121 gctgatattc tgcgccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga
     9181 tagtaatacc cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat
     9241 ctttttgcgc cagccagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag
     9301 caatgtcgat agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg
     9361 gcgaataagc gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac
     9421 cgaggctggc tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac
     9481 gaagtaatga tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt
     9541 ctgtcgatgc aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc
     9601 cattaaggat aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc
     9661 gagttcaggg cgtaggattt cgctacacca cgcagtacga agcgaaaaaa ctgggcttaa
     9721 ccgggtacgc caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag
     9781 ggcaggtgga aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtgg
     9841 aacgggtgct tagcgagccg catcatccct cgggggaatt agccgatttt cgtatccatt
     9901 aaatacattt taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt
     9961 tcggaaacag tcgatataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca
    10021 tcgcttttac cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca
    10081 caaaacgcac cacttcccag tgttctggcg acagcgaaat cccttcgttc tctgcaatca
    10141 ccacagccag cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt
    10201 ctatctcttt accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag
    10261 tgtaacaaaa aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt
    10321 aatcgcggct ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa
    10381 catacaggct aaccgtggca cgaatatagt tcgtttcacc gccatgaatg atgttgctgg
    10441 tttcaaacaa aatagcgcca gaggagatca aaatgaagac cgcgctgatc gccagatgca
    10501 gagcaggcag ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg
    10561 ccatcagcat accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc
    10621 agcagaagaa cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg
    10681 cagacagata ggtgttcaga attggtccga ggatataacc cagaaaaccg gtaaaggcga
    10741 atgcggagat aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat
    10801 acatacccac cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag
    10861 ttgcggtaat cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca
    10921 ccttatgggt gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg
    10981 agtcactctc tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac
    11041 ccagaagttt tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca
    11101 ggtcattaag caaatattac gccctgagaa tttcgacagg caaaagaaaa aggggttagc
    11161 atttagctaa ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt
    11221 tcgggtcgcc ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac
    11281 gcgcactata aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa
    11341 acagccaaaa gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga
    11401 attccttctc ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga
    11461 aaaataggtc catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca
    11521 aaagtttcac tacgctttat taacaatact ttctggcgac gtgcgccagt gcagaaggat
    11581 gagctttcgt tttcagcatc tcacgtgaag cgatggtttg ccttgctaca gggacgtcgc
    11641 ttgccgacca taagcgcccg gtgtcctgcc ggtgtcgcaa ggagaagaga cgtgcgatat
    11701 gaataacgag gaaacatttt accaggccat gcggcgtcag ggcgttaccc ggcgcagctt
    11761 tctcaaatat tgtagtctgg ctgccacgtc gctgggatta ggcgcgggaa tggcaccaaa
    11821 gattgcctgg gcgctggaga acaaaccccg cattccggtg gtatggatcc acggtctgga
    11881 atgcacctgc tgtaccgaat cttttatccg ctccgctcac ccactggcga aggacgtcat
    11941 cctttccctg atttccctcg attatgacga tactttgatg gctgccgccg gaacccaggc
    12001 ggaagaagtc ttcgaagaca tcatcatgca atacaatggc aaatatatcc tcgcagtaga
    12061 aggtaatccg ccgctgggcg agcaggggat gttctgtatc agcagcggtc gaccgtttat
    12121 tgagaaactc aaacgtgccg ctgccggagc cagcgcgatt atcgcctggg gaacctgcgc
    12181 gtcctggggc tgcgtgcagg ccgcgcggcc caatccgacg caggcaacgc ctatcgacaa
    12241 agtcatcacc gacaaaccca ttatcaaagt acctggctgc ccgccgatcc cggatgtgat
    12301 gagcgccatc attacttaca tggtgacctt tgatcgcttg ccagatgtcg acagaatggg
    12361 ccgtccgctg atgttctatg gtcagcgaat ccacgataaa tgctatcgcc gcgcccactt
    12421 cgacgccgga gagttcgtcc agagttggga tgatgacgct gcccgcaaag gttactgcct
    12481 gtacaaaatg ggctgcaaag ggcctaccac ctataacgcc tgttcctcca cacgctggaa
    12541 tgatggcgtt tcttttccaa tccagtctgg tcacggctgc ctgggctgtg cggaaaatgg
    12601 tttctgggat cgcggttcgt tctacagccg cgtggtcgat attccgcaaa tgggtactca
    12661 ttccactgcc gataccgtcg gtttaaccgc gcttggcgtg gtggcagcgg ctgttggtgt
    12721 gcacgcagtc gccagcgccg ttgaccagcg cagacgtcat aaccagcaac ctacagaaac
    12781 cgaacatcag ccaggcaatg aggataaaca ggcatgagca ctcagtacga aactcaggga
    12841 tacaccatca ataatgccgg acgccgcctg gtggtcgacc cgattacgcg catcgaaggc
    12901 cacatgcgct gcgaagtgaa tattaacgat cagaatgtga tcaccaatgc cgtctcctgc
    12961 ggcaccatgt ttcgcgggct ggagatcatc ctacaagggc gcgacccgcg cgatgcgtgg
    13021 gcgttcgttg aacgtatctg cggcgtctgt actggcgtac acgccctggc ttcggtttac
    13081 gccatcgaag atgctatcgg tattaaagtg ccggacaacg ccaatatcat ccgcaacatt
    13141 atgctggcaa cgctctggtg ccacgatcat ctggtgcact tctatcagct tgccgggatg
    13201 gactggatcg atgtgttaga tgcgctgaaa gccgacccgc ggaaaacctc cgaactggcg
    13261 caaagtctct cctcttggcc gaaatcatcc cctggctatt tcttcgacgt acaaaaccgc
    13321 ctgaaaaaat ttgttgaagg cgggcagttg gggatcttcc gcaatggcta ctgggggcac
    13381 ccgcagtaca aactgccacc agaagctaac ctgatgggct ttgcccacta tctcgaagct
    13441 ctcgatttcc agcgtgaaat tgtcaaaatc cacgcggtct ttggcggtaa aaacccgcat
    13501 ccaaactgga ttgtcggcgg gatgccgtgc gccattaaca ttgatgaaag cggcgcggtc
    13561 ggggcagtca atatggaacg cctgaacctg gtgcagtcaa ttatcacccg cacggcggac
    13621 ttcattaaca acgtgatgat ccccgacgcc ttagccatcg gtcagttcaa caaaccgtgg
    13681 agcgaaatcg gcactggtct ttctgataaa tgcgttctca gctacggcgc attcccggat
    13741 attgccaacg actttggcga gaaaagtctg ctgatgcctg gcggcgcggt gattaacggc
    13801 gacttcaaca atgtgctgtc agtggatttg gttgatccgc agcaggtgca ggagtttgtc
    13861 gaccacgcct ggtatcgata tcccaacgat caggtcgggc gtcatccgtt cgatggcatc
    13921 accgacccgt ggtacaaccc cggcgatgtc aaaggcagcg ataccaacat tcagcagctg
    13981 aatgaacagg aacgctactc gtggatcaaa gcgccacgct ggcgcggtaa cgcgatggaa
    14041 gtggggccgc tggcgcgcac gttaatcgct tatcacaaag gcgatgctgc gaccgttgag
    14101 tcggtcgatc gcatgatgtc ggcgttgaac ctgccgcttt ccggtatcca gtcaacgtta
    14161 ggccgcattt tgtgccgcgc gcacgaagcg cagtgggccg caggtaagtt gcagtatttc
    14221 ttcgacaagc tgatgaccaa cctgaaaaac ggcaatctcg ccactgcttc cacggaaaaa
    14281 tgggaacctg caacctggcc gacagagtgc cgtggtgtcg gttttaccga agcgccgcgc
    14341 ggggcgttag gccactgggc cgccattcgc gatggcaaga ttgatctcta ccagtgcgtg
    14401 gtgccgacca cctggaacgc cagcccgcgc gatcccaaag ggcagattgg cgcttatgaa
    14461 gcggcgctga tgaacaccaa aatggcgatc cccgagcaac cgctggagat cctgcgtact
    14521 ctgcacagct ttgacccgtg cctcgcctgt tcaacacacg tgctgggcga cgacggtagc
    14581 gagctgatct ccgtgcaggt gcgttaacag cgaaggagaa tcatcatgca acagaaaagc
    14641 gacaacgttg tcagccacta tgtctttgaa gcgccagtcc gcatctggca ctggttgacg
    14701 gtgttatgca tggcggtgtt gatggtcacc ggatacttta tcggcaagcc gctaccttcc
    14761 gtcagcggcg aggcgacgta tctgttctat atgggctaca tcaggttaat tcacttcagc
    14821 gccgggatgg tttttaccgt ggttttgctg atgcggatct actgggcttt tgttggcaat
    14881 cgatactccc gcgagctgtt tatcgtgccg gtatggcgta aaagctggtg gcagggcgtg
    14941 tggtatgaaa tccgctggta tctgtttctg gcaaaacgtc cgagtgccga tataggccat
    15001 aatcccatcg cccaggcggc gatgttcggc tatttcctga tgtcggtctt tatgatcatc
    15061 actggttttg cgctgtatag cgaacacagc cagtacgcta tttttgcgcc gttccgttat
    15121 gtggtggaat ttttctactg gacgggtggc aactcaatgg acattcacag ctggcatcgg
    15181 ctggggatgt ggctgattgg cgcgtttgtg atcggtcatg tctacatggc gctgcgtgaa
    15241 gacatcatgt ccgacgacac ggtgatctcc accatggtca acggctaccg tagccacaaa
    15301 tttggcaaaa taagtaacaa ggagcgttca tgagcgagca acgcgtggtg gtcatggggc
    15361 tgggcaacct gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa cggctgtatg
    15421 cccattacca ctggcccgag tatgtggaga ttgtcgatgg cggtactcag ggactgaact
    15481 tgctggggta tgtcgaaagc gccagccatc tgttgattct cgatgccatt gaccacgggc
    15541 tggaacctgg aacgctgcga acctatgccg gagaacgcat tccggcttat ctcagcgcga
    15601 agaaaatgag cctgcatcag aacagtttct ccgaagtgtt ggcgctggcg gatatccgcg
    15661 gacatctgcc agcacatatt gccctcgtcg gtctgcaacc cgcaatgctc gacgactacg
    15721 gcggtagcct gagcgaactg gcacgggagc aactgcccgc tgcggaacag gcggcgctgg
    15781 cgcagctggc tgcgtgggga attgtgccgc aaccggctaa tgaatcgcgc tgtctcaatt
    15841 atgactgtct gtcgatggaa aattacgaag gcgttcgctt gcgccagtac cggatgacac
    15901 aggaggagca gggatgagca acgacacgcc atttgatgcg ttgtggcaac gaatgctggc
    15961 gcgcggctgg acgccagtca gtgaatcccg tcttgacgac tggcttacgc aagcgccaga
    16021 cggcgtggtg ttattaagca gtgacccgaa acgcacgcca gaggtcagcg ataatccggt
    16081 aatgattggc gaattactgc gcgagtttcc cgactataca tggcaggtgg cgattgctga
    16141 ccttgagcag agcgaagcca tcggcgatcg ctttggcgtc tttcgctttc ctgccacttt
    16201 agtgtttacc ggcggaaact atcgcggcgt gctgaatggt attcacccgt gggcggaact
    16261 gataaacctg atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat gagcgaaact
    16321 tttttccatc tgctggggcc aggaacacaa ccgaacgatg acagtttcag catgaatcca
    16381 ctgccgatca cctgtcaggt gaatgatgaa ccgagtatgg cggccctgga gcaatgtgct
    16441 cacagcccgc aggtgattgc gctgttaaac gagttacaac atcaactaag cgaacgccaa
    16501 ccgccgttgg gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga cgatcgtcac
    16561 tttatcaata cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca ggctgacgac
    16621 agtgaaagtg aaatacagga ggcgatcttc tgcggattat ggcgggtgcg cagacgtcgc
    16681 ggcgaaaagt tgctggagga caaactggag gctggctgcg cgccgctggc gttgtggcag
    16741 gcggcaacgc aaaatctctt gccgacagat tcgctgttac cgccgcccat tgatggcctg
    16801 atgaatggcc taccgttggc gcatgagtta ctggcacatg tacgtaaccc cgacgcgcag
    16861 ccgcacagca ttaatctgac gcaattaccc atcagcgagg ctgatcggct ttttctctca
    16921 cgtctctgtg ggccgggaaa tattcagatt cgtaccattg gctatggcga gagctatatc
    16981 aacgccacgg ggttacgcca tgtctggcat ttacgctgta cggacacctt aaaaggcccg
    17041 ttactggaaa gttatgaaat ctgcccaata ccggaagtgg tgctggcagc gccagaagat
    17101 ttggtcgact ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga agctgcaccg
    17161 acgtaaaaag acggtaagta tcgctttcag tcttatgaat atcgcaatcg gcgaatacct
    17221 ctggtcgtag agtttcagga taaagaggga gatctaccat tatcgggtta tttttctctc
    17281 ttcgcctaca ggagtgcgca tgtgggatgt cattgattta tcgcgctggc agtttgctct
    17341 gaccgcgctg tatcactttt tatttgtacc cctaaccttg gggctgattt ttttgctggc
    17401 tatgatggaa accatttacg tggtcaccgg caaaacaatc taccgcgata tgacgcgctt
    17461 ctggggtaag ctcttcggta tcaattttgc tcttggcgtg gctaccggcc tgaccatgga
    17521 gtttcagttt ggtactaact ggtcattcta ttccaactat gtgggcgata tttttggcgc
    17581 accactggcg atggaagcat taatggcctt cttcctcgaa tccacctttg tcgggctgtt
    17641 cttcttcggc tggcaacggc tgaataaata ccagcacctg ctggtgacgt ggctggtggc
    17701 gttcggttca aatctctctg cgttgtggat attgaatgcc aacggttgga tgcaataccc
    17761 gaccggtgcg cattttgata tcgacaccct gcgtatggag atgaccagct tcagcgagct
    17821 ggtctttaat ccggtcagcc aggtgaaatt tgtgcacacc gtaatggcgg gctacgtgac
    17881 cggggccatg tttattatgg cgatcagcgc ctggtattta ctgcgcggac ggaagcgcga
    17941 tgtcgcatta cgctcgtttg ccatcggttc cgtcttcggt actctggcga ttatcggtac
    18001 cctgcaactc ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg taaaactggc
    18061 ggcgatggaa ggggagtggc aaacggaacc tgcacctgca ccgttccatg tggttgcctg
    18121 gccggaacag gatcaagagc gtaacgcctt tgccctcaaa attcccgcgc tgctagggat
    18181 cctcgccact cactcattag ataaacccgt gccgggtctg aagaatttga tggctgaaac
    18241 ctacccacgc ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa tatcgcaagg
    18301 caatcgtgag ccgcatgtgt tgcaggcatt ccgggaactg gaaggtgacc tgggctacgg
    18361 catgttgctc tcccgctatg cgccggatat gaatcatgtc acagccgcac agtaccaggc
    18421 ggcgatgcgt ggcgcgatac ctcaggttgc gccggtattc tggagtttcc gcatcatggt
    18481 gggctgtggt tccctgctgc tactggtgat gctgattgcg cttgtccaga cgctgcgtgg
    18541 caaaatcgac cagcatcgct gggtgctgaa aatggcgctc tggagtttgc cgttgccgtg
    18601 gattgcgatt gaagccgggt ggtttatgac cgagtttggt cgtcagccgt gggcgataca
    18661 ggacatctta ccgacatact ccgcgcactc cgctttaacc acaggacaac tggctttctc
    18721 actgatcatg atcgtagggc tttacaccct gttcttaatc gccgaagtct acctgatgca
    18781 gaaatatgcc cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc agcaacaggg
    18841 gtaaaggaga aaatcatgtt tgattatgaa acattgcgct tcatctggtg gctgctgatt
    18901 ggcgtgatcc tggtggtctt tatgatctcc gacggatttg acatggggat cggctgtctg
    18961 ctgccgctgg tggcgcgtaa tgatgatgaa cgccggatag tgataaacag cgttggtgca
    19021 cactgggaag gcaaccaggt ctggttgatc ctcgctggtg gggcattatt tgccgcctgg
    19081 cccagagtgt atgcagcggc gttttccggc ttttatgtgg cgatgatcct ggtgctgtgc
    19141 tcactgttct tccgcccgct ggcctttgat tatcgcggaa aaatcgccga tgcacgctgg
    19201 cgtaaaatgt gggacgccgg tctggtcatc ggcagtctgg tgccgccggt agtcttcggt
    19261 atcgccttcg gcaacttgtt gctcggcgtg ccgtttgcct tcacactgca attacgcgtg
    19321 gagtatctcg gcagcttctg gcaactgctg acgccattcc ctttattgtg cggattgctc
    19381 agccttggga tggtgatttt gcaaggtggc gtctggttac aactgaaaac tgttggtgtg
    19441 attcatctgc gttcacagct ggccaccaaa cgcgctgcac tgttggtgat gctgtgcttt
    19501 ttgctggtgg gttactggct gtgggtcggt attgatggct ttgtactgct cgcccaggat
    19561 gctaacggtc cttccaatcc gttaatgaaa ctggtggcag tgctacctgg tgcctggatg
    19621 aataatttcg tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt cttctgccca
    19681 ttgctgacgg tgatggcgat ttatcgtggt cgcccgggtt ggggattttt gatggcatca
    19741 ttgatgcaat ttggcgtgat tttcacggca ggcatcacgc tgttcccctt tgtcatgccg
    19801 tcaagcgtga gtccgatctc cagcctgacg ttgtgggaca gcacttccag tcagctgacg
    19861 ctgagcatta tgttggtaat cgtgctgata tttttgccta ttgtgttgct ctacactctc
    19921 tggagctact acaaaatgtg gggacgcatg acgacagaaa ctctccgccg taacgaaaac
    19981 gagttgtact aaggagcagg aacaatgtgg tatttacttt ggttcgtcgg cattttgttg
    20041 atgtgttcgc tctccaccct tgtgttggta tggctggacc cgcgtctgaa aagttaacga
    20101 acgtaggcct gatgcggcgc attagcatcg catcaggcaa tcaataatgt cagatatgaa
    20161 aagcggaaac atatcgatga aagcgatctt aatcccattt ttatctcttc tgattccgtt
    20221 aaccccgcaa tctgcattcg ctcagagtga gccggagctg aagctggaaa gtgtggtgat
    20281 tgtcagtcgt catggtgtgc gtgctccaac caaggccacg caactgatgc aggatgtcac
    20341 cccagacgca tggccaacct ggccggtaaa actgggttgg ctgacaccgc gcggtggtga
    20401 gctaatcgcc tatctcggac attaccaacg ccagcgtctg gtagccgacg gattgctggc
    20461 gaaaaagggc tgcccgcagt ctggtcaggt cgcgattatt gctgatgtcg acgagcgtac
    20521 ccgtaaaaca ggcgaagcct tcgccgccgg gctggcacct gactgtgcaa taaccgtaca
    20581 tacccaggca gatacgtcca gtcccgatcc gttatttaat cctctaaaaa ctggcgtttg
    20641 ccaactggat aacgcgaacg tgactgacgc gatcctcagc agggcaggag ggtcaattgc
    20701 tgactttacc gggcatcggc aaacggcgtt tcgcgaactg gaacgggtgc ttaattttcc
    20761 gcaatcaaac ttgtgcctta aacgtgagaa acaggacgaa agctgttcat taacgcaggc
    20821 attaccatcg gaactcaagg tgagcgccga caatgtctca ttaaccggtg cggtaagcct
    20881 cgcatcaatg ctgacggaga tatttctcct gcaacaagca cagggaatgc cggagccggg
    20941 gtggggaagg atcaccgatt cacaccagtg gaacaccttg ctaagtttgc ataacgcgca
    21001 attttatttg ctacaacgca cgccagaggt tgcccgcagc cgcgccaccc cgttattgga
    21061 tttgatcatg gcagcgttga cgccccatcc accgcaaaaa caggcgtatg gtgtgacatt
    21121 acccacttca gtactgttta ttgccggaca tgatactaat ctggcaaatc tcggcggcgc
    21181 actggagctc aactggacgc ttcccggtca gccggataac acgccgccag gtggtgaact
    21241 ggtgtttgaa cgctggcgtc ggctaagcga taacagccag tggattcagg tttcgctggt
    21301 cttccagact ttacagcaga tgcgtgataa aacgccgctg tcattaaata cgccgcccgg
    21361 agaggtgaaa ctgaccctgg caggatgtga agagcgaaat gcgcagggca tgtgttcgtt
    21421 ggcaggtttt acgcaaatcg tgaatgaagc acgcataccg gcgtgcagtt tgtaatgcat
    21481 aaaaaagagc attcagttac ctgaatgctc tgaggctgat gacaaacgaa gaactgtcta
    21541 atgcgtagac cggaaaaggc gttcacgccg catccggcca ctttcagttt tactctttct
    21601 cggagtaact ataaccgtaa tagttatagc cgtaactgta agcggtgctg gcgcgtttaa
    21661 tcacaccatt gaggatagcg cctttaatat tgacgcctgc ctgttccaga cgctgcattg
    21721 acaaactcac ctctttggcg gtgttcaagc caaaacgcgc aaccagcagg ctggtgccaa
    21781 cagaacgccc cacgaccgcg gcatcactca ccgccagcat cggcggcgta tcgacaatca
    21841 ccagatcgta atggtcgttc gcccattcca gtaattgacg catccgatcg cgcatcagca
    21901 gttcagacgg gttaggtggc acctgaccgc gagtaatcac atcaaagcct cctttgccaa
    21961 aatgctggat cactttgttg agctcatctt tacctgccag atattccgac aagccatgtt
    22021 cattactcac ggtaaacagg ttatgcgaat aaccacggcg taagtcggca tcaataaata
    22081 acactttttg atcggactgg gcgatcaccg ctgccagagt tgaactgaca aacgttttac
    22141 cactgtctgg cgtcgcaccg gtgatcatca gaatgttatt ctccgtctcc atcatagcga
    22201 agtgcagact ggttcgtagc gcacgtacgg cttccacagc agaatccgcc gggttatcca
    22261 ccgccaggaa ggggatattt ttagtacgat ggcgctgctg attagaaaat aaatttttct
    22321 tacgcagacg ggtacgttta tccagccact cggacattgg gatagtggca taaacgctga
    22381 tgccgtgctc ttccagttgt tccggggctt ctacaccacg acgcaacatc gcacgcgcca
    22441 gcacggcacc caccgaaata aacaggccaa gaataaaacc aagcaccacg ttcaacgctt
    22501 ttttcggttt cactggctgc ggctgagtga ctgccgggtc gataatccgc acgttaccaa
    22561 tggcactgga tttcgaaata ctcaactcct gctggcggtt aagtaattgc agatataccg
    22621 cacggcccgc ttctacgtca cggcttaaac gtaacacttc ctgttgagtg gaaggcattg
    22681 ccgatacccg cttattcagg cgtttgcgtt cttgctccag cgtctggcgt ttttccagca
    22741 gcgcacgata agttgggtga tctttcttat acagctggga gatctctgcc tcgcggaaag
    22801 tcagctcatt gagttgatta tcaacgttca caatctgctc aagaacggct ttggcttcca
    22861 ggttaaggtc aaccgaatcg cgctgctggc gataaacgtt gagtttttct tccgcctggt
    22921 ccagctcgct gcgcacttca ggcaactggc gctgtaagaa ttcaaggctt tgtgaatcct
    22981 gcgccgcctg gcgagcgata ttctgttgca aatagttgtt agcgatgctg ttcagaatac
    23041 gagtaatcaa ctggggatca tcaccagtca tggtaagttc cagcatcccg ctttctttac
    23101 tgcgttcgct aacggtaaag gtttcctgca atgcgttaat tgcttccagt tcggtacgct
    23161 ggctcaggac aaactgtgtt cctggtttgg ccttaatgtc cgcgatagtc agcgcaacgc
    23221 catctttttc cagacgctgg ccgaccatac cattgacggt gaactcttca ccttccagtg
    23281 tatagtggcc gttttcccca accgtgagtg tcagttgctg atcctgacca ttcagttgtg
    23341 gaatatgcat ccagctgatc gccagctcac ctggtttttc tttggttaat ctcgcccagc
    23401 cgcgacccac aatcggaaaa tacttctgct caactatgtc gcgcagattc agttcagcaa
    23461 tggttttacc gagaatcatg cgcgattgca gcagttggat ctccggtgca gactcgggcg
    23521 atgagttagg gatcatatcg ctcaggccgc tgagaatggc attgccctgt ttttgctcaa
    23581 cctggaccag agtatctgcc tgataaattg gtgtgcttaa cagcgagtaa gcgacagcga
    23641 tcagcgtgaa taacgcggtc acgctgataa taaacttacg gtgatcccat aactcgccga
    23701 ccagacgaag cagatcgatc tcattttcct gagtgctgcc tggtggcgta ttcatatttt
    23761 tagttgtcat cgtattttat aaactcaaat taccggctga ggcgcttcgc ccattcctga
    23821 ctggcgcgct ccaacatacc gtagacatgt tcaaatgcgt cctgactttt acgataggga
    23881 tccgggatct ctttctgttc cagccattgc ccaaacagca ttgtcttccc gcggacctcg
    23941 ggagcgattg cggtaacctg agcaatatgc tctgactcca ttgccagaat cagatcgtaa
    24001 tttcgtgcca tctcagcagt gagcttacgt ccggcatgtc cttcaagaga tacgccatga
    24061 ttagcggcaa catctgctgc tgtcgcatct gcagggtgtt ttaccagacc atgaacacct
    24121 gccgatttga cttttacgcc tggcagacgt ttacgcagca agcgctcgcc aatgggcgaa
    24181 cggcaaatgt tgccggtata aaccaccagg attgagttaa attttagttg ggccatgttt
    24241 gaatccaccg tgaagtctct gtcaggtcat gcaccccgga aatcgtcggt accagcagag
    24301 aaatcacacg gttccaacga gccagcggcg cagtagtgac ataaacaatg tcgtaaggtt
    24361 caagctggaa ttccgttccg agtatcattg ccgatgcgtc cttcgcattc agctgataaa
    24421 tattggcgat tttgccattc tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg
    24481 catcagcgac atcctgattc ataccctcag cgttacccag cgcttccgcc agggtcatac
    24541 cgctgcggtc cattttcagc gtgctctgtt tacccacttc acccatcacg aagactttca
    24601 gatcatcgtt gcgcggaata aataaaatat cgcccgggtg cagcagtttg ttctggcgca
    24661 agtcgccacg ctgcatcagc gcatagaggt tcactttggt ctttacgcca ttctgggtga
    24721 gcaccacgtt gcgccagtct gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca
    24781 tgatggtcag aggaatattg gtaatcggct gctgacccga tttgctgact tcgccggtga
    24841 cgcacgcttt ttgcgatcgg aacgccgcga cgctgacatc gacctgcggg ctttcaatga
    24901 ctgaatccag acgcgctgta atctcgttgc gtacctgggt aagtgttttc ccggccactt
    24961 tcaggcgacc aatatagggg tagaaaattg caccatcagc gttaacccag ttgccggtat
    25021 cactggcgct acggtattgc cctgcggggg tggttagttc cgggtgatcc cagaccgtaa
    25081 ccatcaacac atcaccaatc ccgatacgat attcatagtt tgcgatttgc tgctccagct
    25141 ccgggttagc ctgcgacata atcggtttgg cgcgcaactg atcaatcaat cctggcgtga
    25201 cgggatagat attgaccatt ttgtccaggt catactgatt gtcaggtagt tcaatgacat
    25261 ccttgttact ggtcgataaa ttctgaccag gaacaagggt acatgctgtc aaagacagaa
    25321 cagccaatgt cagtaccgaa aacttaaaaa tgttcttttt cattggttta tatttatcag
    25381 tcaatgtaat gttatgaatt acgtcaggtt attgtccaac cggtacttct cgctctgccg
    25441 tcattggata gagctggtat ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg
    25501 tccacgaaac caccgcgcgg ttgcggttag gtccaatggt catcaagtcg aatggaatcg
    25561 agatataaaa acctttacta aagccgcctt cgccgtagtc atctttagat acgttactga
    25621 ttgccgccca tacccctacc gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg
    25681 cgcctttatc ttttgccaga tattgcccaa cgctaagttt catcagtacg ccattgagcg
    25741 tcggcgggtt ccagtaagcc gtcacgaaac ctgttggcgt ggaataatcg gtgaaacgca
    25801 tcatgttgtc ccagtcacgt tgcttcacgt agttaacgtc cacacccagc gcccagcagg
    25861 catctagcgg gcgataaagc agctcggaac cgacaccggc gtacatcgtt tccagataac
    25921 cgccatacac ctggccatag aaaccattgc ctaagtcggc aaagtagttc gcctgcaagt
    25981 tgttgagata aacgtcattg cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg
    26041 tagagtccgc gggcaacagc gaagacttaa acttgtcgta gttgttgtaa atattggtga
    26101 aaataccgcc atcaagcagc aggtggtcgg taaaccagta gcgggcactg gacatcagcc
    26161 ccagctggaa catatagaaa tcttccgggc cgcccagcga ctgtgaaagt gttgggttga
    26221 aagagtagct aaagcgatct tcacgaatac ggtagccccg accaaaggca gaaagatctt
    26281 ctgcttcaac acgttgttgt tgaagttgct ctgattgacc aggcgctgta ccagccagct
    26341 gtttgcgcag gctggctacg tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt
    26401 gcgtcacgct aatcttctca acgccttgcg gcaggttatt caccagaatc cggttggcac
    26461 gatcgaccgc ttcgcgagaa tcacggtatt tgtattgttg accagacata tacagcgtct
    26521 tatcgcgcag ctgaatttcc ggtgcttcaa aacctgcgtt gtacttcagc gcggtaagtt
    26581 ggttcgctac tgtggtgtac tgcaatcctt cagattcagg cgcaggttga tatgccggtt
    26641 ttggcgtatc gcgcagggca gggcgcagat cgttgaaatt ggtccgtaac gtgaagccaa
    26701 acatcaacgt gttaccgcgt tcataactca ggttgagatc tgcccagctg gcagcgcgat
    26761 aaactgcgcc gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa tcattctggt
    26821 aattgttgcc atcgtattcg agtttcagac gcaggggatt ccacggcgtt tgatactcaa
    26881 tgccgccaaa gatggaagcc gggccacgaa agatatcgct aaagctgata tcgcccgcat
    26941 cgtgagactc tgcgcgatga caatatttat cgctcacccg gcaaaacggg ttggtaatat
    27001 tgcccgcatt accggcgtag ccccatgcca tcccgagggt gaaatcaaat ggccccgctt
    27061 gcttgctggc caccagatac tcaccgtcaa acagacccgt accagcaata tcacgtttac
    27121 caaacgccac ttgcggtagc cagtaacctt cttcccacag gcgaagttta aaatcgaatg
    27181 atttgtcttt gtatgactga tcgccgctga aatcttccca ctggctatat ttgcgtgtgc
    27241 gcacatccgt ataacgaatg gtgccttcca gccatgggaa cagcgccacg gaggtggagt
    27301 agaaccggta ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc
    27361 gcgcatttgg catctgcaac aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg
    27421 gatccgggta ggttaatact tcagcatgac aggcactgga gacggcaatg gccaggcagc
    27481 ttaaaagata agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat
    27541 ctggtcattt aaatcagcat atttttcggg cagtacatgc gcagaaaagc ccaaccaaag
    27601 ctggctgcca ggcggtggtt ccacatgtct tttattccac agggcaacag gggcgacgac
    27661 agtctcaccc tcaggcgtaa tcaccataac gttattcttg tccgcgcctg caaggcgggg
    27721 gtgatcttgc agataatcgg taacgctacg gcctgcctgc cagggcaatt gcccggcccc
    27781 agaaaccgca cccagcagag taatagtcac cggacgctgt acggtataga gcgtatagtc
    27841 cccgaccaac gggggattgc tgttctcatc cactcgtaca aaatcagggt ccagtttgac
    27901 cggtaagcga ccagtgatat tgagattgag taactgctgg cgcacggatt taatcgttgc
    27961 cgccacatcg tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga catgttggta
    28021 gtctttaagc gctttagctt tcgccgcaga gtcggtcagc aaggcaccag gccaccagag
    28081 gcgatcgcgc agttgcggtt gtgtcaccaa ctggacaaca ttttccatgg ggccaacgga
    28141 aagtgtctgt tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg
    28201 tgtcattacg taaagtacgc tggcaatgaa atacgactgt aatttattca tggtgcgggt
    28261 ttaagaaacg tcatttctac gggaataacc cctgcgccta acatttgccg actttgacga
    28321 acctggccag tagccgaatc gacccagaat gtgttttgcc actgtttttc tggccgcgtg
    28381 gaagtcacct cttcctgcca gacattacac agcacattgc gacccgcaat attcagcgtc
    28441 tctaatccag caaaggaaaa agtagaactc agggtcgcag agcgaaaatg gttgtcttca
    28501 ctccagagaa tatcgcgcgt ccagcggctg ccatctttta ttgccagcgc gttacgcaga
    28561 gggtcctgcc cggaatttgt cacttccagc agattattat taagtttgac ggtttttaaa
    28621 agacgtccat tgtgggtaac cagcatggcg ttatcctggg ataaccattt gctttgttct
    28681 tgttctatat atcccagtac cacaaagatt cgttgccctt cattaaggcg taaatacatc
    28741 gtggaataag gcaacgcctg gatctgctga tccgctacgg tgatatcctg attatcgaaa
    28801 aggctggcgc gaaatgtatc gaccatactt tgctggctgt gcgtacatcc cgcaagaaat
    28861 agtgcgaaaa tcgataaaat aagagggcgc acctttaatc cttgaaaggt taattaaaaa
    28921 aatgcaaatc tctaaagccc ggtacgaata tcgcaaccgg gtatcattta actttaattt
    28981 cgacgcgtta aagcagtatt agcgtgtagt ggacgtggtg gttgaggtgt tatgagaacc
    29041 gccatcgcca ctgtcatttg ctgcaacaac tgccaacgtc gccacaccaa cagccgttgc
    29101 cacaccgaca actgtcgccg tgccgacagc gccagcagcc acgccaccag tggtagtagc
    29161 agtcgtggtt gcgccggtag tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt
    29221 cccgtttgtt gcttcagggg cagcaaacgc agctggcgtc gtcaacatga aggccataa
//
LOCUS       DAJSKJ010000027        28462 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000027 DAJSKJ010000000
VERSION     DAJSKJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.027"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1209
                     /locus_tag="ON345_001066"
     CDS             <1..1209
                     /locus_tag="ON345_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR3071163.1"
                     /translation="TVNDDANDVAVTESYKPIWNWKISQYNVFDIKFETMIKPQFADR
                     TYFSNCSPVDPTSTRPTYFGDTDGSVGVVLYALFATGHLGIMAEGENFLSQLLNIEDE
                     VLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQTGD
                     LFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGI
                     KDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVELAAIINKK
                     HHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGG
                     RECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMP
                     LIRRRTMLQL"
     gene            1575..2642
                     /locus_tag="ON345_001067"
     CDS             1575..2642
                     /locus_tag="ON345_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR3071164.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAVQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2639..5374
                     /gene="rbbA"
                     /locus_tag="ON345_001068"
     CDS             2639..5374
                     /gene="rbbA"
                     /locus_tag="ON345_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR3071165.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYKPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPAIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQRPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTTVSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5374..6498
                     /locus_tag="ON345_001069"
     CDS             5374..6498
                     /locus_tag="ON345_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3071166.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGIPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6571..6846
                     /locus_tag="ON345_001070"
     CDS             6571..6846
                     /locus_tag="ON345_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR3071167.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6843..7202
                     /locus_tag="ON345_001071"
     CDS             6843..7202
                     /locus_tag="ON345_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3071168.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7322..7723)
                     /gene="nikR"
                     /locus_tag="ON345_001072"
     CDS             complement(7322..7723)
                     /gene="nikR"
                     /locus_tag="ON345_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR3071169.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7729..8535)
                     /gene="nikE"
                     /locus_tag="ON345_001073"
     CDS             complement(7729..8535)
                     /gene="nikE"
                     /locus_tag="ON345_001073"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR3071170.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(8532..9296)
                     /gene="nikD"
                     /locus_tag="ON345_001074"
     CDS             complement(8532..9296)
                     /gene="nikD"
                     /locus_tag="ON345_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR3071171.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9296..10129)
                     /gene="nikC"
                     /locus_tag="ON345_001075"
     CDS             complement(9296..10129)
                     /gene="nikC"
                     /locus_tag="ON345_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR3071172.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10126..11070)
                     /gene="nikB"
                     /locus_tag="ON345_001076"
     CDS             complement(10126..11070)
                     /gene="nikB"
                     /locus_tag="ON345_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR3071173.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11070..12644)
                     /gene="nikA"
                     /locus_tag="ON345_001077"
     CDS             complement(11070..12644)
                     /gene="nikA"
                     /locus_tag="ON345_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR3071174.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TTKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12755..13342)
                     /gene="acpT"
                     /locus_tag="ON345_001078"
     CDS             complement(12755..13342)
                     /gene="acpT"
                     /locus_tag="ON345_001078"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR3071175.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13397..14446)
                     /locus_tag="ON345_001079"
     CDS             complement(13397..14446)
                     /locus_tag="ON345_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3071176.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14578..15795
                     /locus_tag="ON345_001080"
     CDS             14578..15795
                     /locus_tag="ON345_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3071177.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15799..16356)
                     /gene="dcrB"
                     /locus_tag="ON345_001081"
     CDS             complement(15799..16356)
                     /gene="dcrB"
                     /locus_tag="ON345_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR3071178.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPTDDQQKAQTTAENIINTLVIQ"
     gene            complement(16429..17094)
                     /locus_tag="ON345_001082"
     CDS             complement(16429..17094)
                     /locus_tag="ON345_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR3071179.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17315..17560
                     /gene="tusA"
                     /locus_tag="ON345_001083"
     CDS             17315..17560
                     /gene="tusA"
                     /locus_tag="ON345_001083"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR3071180.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17662..19860)
                     /gene="zntA"
                     /locus_tag="ON345_001084"
     CDS             complement(17662..19860)
                     /gene="zntA"
                     /locus_tag="ON345_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR3071181.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWSLEQFNHPFGQLAFIATT
                     LAGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19934..20560)
                     /locus_tag="ON345_001085"
     CDS             complement(19934..20560)
                     /locus_tag="ON345_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR3071182.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20701..21060
                     /locus_tag="ON345_001086"
     CDS             20701..21060
                     /locus_tag="ON345_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR3071183.1"
                     /translation="MSKPTLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21063..21332)
                     /locus_tag="ON345_001087"
     CDS             complement(21063..21332)
                     /locus_tag="ON345_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR3071184.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21322..21918)
                     /gene="rsmD"
                     /locus_tag="ON345_001088"
     CDS             complement(21322..21918)
                     /gene="rsmD"
                     /locus_tag="ON345_001088"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR3071185.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22068..23561
                     /gene="ftsY"
                     /locus_tag="ON345_001089"
     CDS             22068..23561
                     /gene="ftsY"
                     /locus_tag="ON345_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR3071186.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETA
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23564..24232
                     /gene="ftsE"
                     /locus_tag="ON345_001090"
     CDS             23564..24232
                     /gene="ftsE"
                     /locus_tag="ON345_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR3071187.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24225..25283
                     /gene="ftsX"
                     /locus_tag="ON345_001091"
     CDS             24225..25283
                     /gene="ftsX"
                     /locus_tag="ON345_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR3071188.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25528..26382
                     /gene="rpoH"
                     /locus_tag="ON345_001092"
     CDS             25528..26382
                     /gene="rpoH"
                     /locus_tag="ON345_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR3071189.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26653..27756
                     /gene="livJ"
                     /locus_tag="ON345_001093"
     CDS             26653..27756
                     /gene="livJ"
                     /locus_tag="ON345_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR3071190.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27955..28338)
                     /gene="panM"
                     /locus_tag="ON345_001094"
     CDS             complement(27955..28338)
                     /gene="panM"
                     /locus_tag="ON345_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR3071191.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6785 a         7340 c         7869 g         6468 t
ORIGIN      
        1 acagtaaatg atgatgctaa tgatgttgca gttactgagt cgtacaaacc catctggaac
       61 tggaaaatat cgcaatacaa tgtatttgat attaaattcg aaacaatgat aaaaccccag
      121 ttcgctgaca gaacctattt ctccaattgt tcacctgttg atcccacaag taccaggccg
      181 acctattttg gcgataccga tggctcagtc ggtgtagttt tgtacgcgct ttttgcaacg
      241 ggacatttgg gaatcatggc tgaaggagaa aatttcctta gccagttgct taatatagaa
      301 gatgaagttc tcaacgtatt gctaagggag aactttaatg agcaacttga cactaatgtt
      361 aatataataa tcagtatcct gaacagaaga gacattgttt tagaaagtct tcagccttat
      421 ttggttatta ataaagacgc agtcacgcca tgtacgtttc tgggcgacca gactggcgat
      481 ctatttagta atatctgtgg cgataaattt atcatcgatt tattaaaacg cataatgagt
      541 atcaatgaaa acgttcatgt attagctgga aatcatgaaa cgaattgcaa tggtaactat
      601 atgcagaact tcacgcgcat gaaaccactt gatgaggata cctactctgg gataaaagat
      661 tatcctgtat gtttctatga ccccaaatac aaaataatgg caaatcatca tgggataact
      721 tttgatgatc agcgaaaacg ttacattata ggaccgataa cagtctctag tgatgaaatg
      781 accaatgctc tcgatccggt cgaattagcg gcaattatca ataaaaagca ccatgctgta
      841 attaatggca aaaagtttaa aaatagccgg gctatctctt gtcgatcctt taatcgttat
      901 ttttctgttt cgacagatta cagacctaaa cttgaagcac tattagcatg ctcacaaatg
      961 ttaggtataa atcaagttgt tgcacataac ggtaatggtg gaagagaatg tataggtgaa
     1021 acggggacag ttttaggtct taacgcccgt gacagtaaac atgctggaag aatgttcagc
     1081 atgcataatt gccaaattaa ccctggtgca gggcctgaga taaccactcc ctggaagtct
     1141 taccagcatg aaaaaaacag aaatggactt atgcctttga ttagaaggcg cacaatgtta
     1201 cagctctgaa ccatagacaa catgaatatt aatgttacta tctggttgag actgttgata
     1261 aaacgtaaaa aggaatgctc tgttcctgca actgaacagg cgtgaaacgc gatccaacag
     1321 gatggatacc gttttgtcag ccagttttat agatcacact tatgagtatg cgtgtttcat
     1381 ttacggccag gaatgctgat gatgacaaca ttactacccg ttttcactaa gccttccccg
     1441 ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt tccttcttat cccggatgga
     1501 agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc gtcgtttgct tacagggatt
     1561 atggcgggcc gattatggat aagagtaagc gccatctggc gtggtgggtt gtcggggcac
     1621 tggcggtggc tgccgtcgtg gcgtggtggc tgttgcgccc ggcgggtgtg ccggaaggct
     1681 ttgctgtcag caatgggcgc attgaagcga cggaagtgga tatcgccagc aaaattgccg
     1741 ggcgtatcga caccattctg gtgaaagaag gccagtttgt tcgcgaaggt gaagtgctgg
     1801 cgaagatgga tactcgcgtg ttgcaggaac agcgactgga agccatcgcg caaatcaaag
     1861 aggcacaaag cgccgttgcc gccgcgcagg ctttgctgga gcaacgacaa agcgaaactc
     1921 gcgccgcgca gtcgctggtt aatcaacgcc aggcagaact ggactccgta gcaaaacgtc
     1981 atatgcgttc ccgttcactg gcccaacgag gggctatttc tgcgcaacag ctggatgacg
     2041 accgcgccgc cgctgagagc gcccgagctg cgctggaatc ggcaaaagct caggtttctg
     2101 cttctaaagc cgctatagag gcggcacgta ccaatatcat tcaggcgcaa acccgcgtcg
     2161 aagcggtaca agccactgaa cggcgcattg ccgcagatat cgatgacagc gaactgaaag
     2221 ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc aggggaagtg ctggcggcag
     2281 gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta tatgaccttc ttcctgccaa
     2341 ccgaacaggc gggcacgctg aaactgggtg gtgaagcccg gctgatcctc gatgccgcgc
     2401 cagatctgcg tattccggcg accatcagtt ttgtcgccag tgtcgcccag ttcacgccaa
     2461 aaaccgtcga aaccagcgat gaacggctga aactgatgtt ccgcgtcaaa gcgcgtatcc
     2521 caccggaatt actccagcag catctggaat atgtcaaaac cggtttaccg ggcgtagcgt
     2581 gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct ggtggtgagg ttgccgcaat
     2641 gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa ctggcgggcg tgagccagca
     2701 ttatggaaaa accgttgcgc tgaacaatat tactctcgat attccggccc gctgtatggt
     2761 cgggctgatt ggtccggatg gcgtcgggaa gtcgagctta ttgtcgttga tttccggtgc
     2821 ccgcgtcatt gagcagggca atgtgatggt gctgggcggc gatatgcgcg acccgaggca
     2881 tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag gggctgggca aaaacctcta
     2941 ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct cgcctgttcg gtcacgacaa
     3001 agcggagcgg gaagtgcgaa ttaatgagct gctgaccagc acggggttag caccgtttcg
     3061 cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa aaacttgggc tgtgctgcgc
     3121 gttaatccac gacccggaac tgttgatcct tgatgagcca acaacggggg ttgacccgct
     3181 ctcccgcgcc cagttctggg atctgatcga cagtattcgc cagcggcaga gcaatatgag
     3241 cgtgctggtc gccaccgcct acatggaaga ggccgaacgc ttcgactggt tggtggcgat
     3301 gaatgccgga gaagtgctgg caactggaag cgccgaagag ttgcggcagc aaacgcaaag
     3361 cgccacgctg gaagaggcgt ttataaatct gttaccgcaa gcgcaacgcc aggcgcatca
     3421 ggcggtagtg atcccaccgt ataaacctga aaatgcagag attgccatcg aagcgcgcga
     3481 tctgaccatg cgttttggtt ccttcgttgc cgttgatcac gtcaatttcc gcattccacg
     3541 cggggagatt tttggttttc ttggatcgaa cggctgcggt aaatccacca ccatgaaaat
     3601 gctcaccgga ctgctgcccg ccagcgaagg tgaggcgtgg ctgtttgggc aaccggttga
     3661 tccaaaagat atcgataccc gccgtcgtgt gggctatatg tcgcaggcgt tttcgctcta
     3721 taacgaactc accgtgcggc aaaacctgga gttacatgcc cgtttgtttc acatcccgga
     3781 agcggaaatt cccgcgcgag tggctgaaat gagcgagcgt tttaagctca acgacgttga
     3841 agatgttctg ccggagtcat tgccgctcgg cattcgccag cggctttcgc tggcggtggc
     3901 ggtgattcat cgcccggaga tgttaatcct cgatgagcct acttctggtg tcgatccggt
     3961 ggcgagggat atgttctggc agttgatggt cgatctctcg cgccaggaca aagtgactat
     4021 cttcatctcc acccacttta tgaacgaagc ggaacgttgc gaccgcatct cactgatgca
     4081 cgccggaaaa gtgcttgcca gcggtacacc gcaggaactg gttgagaaac gcggagccgc
     4141 cagtctggaa gaggcattta tcgcctattt gcaggaagcg gcagggcaga gcaacgaagc
     4201 cgaagcgccg ccggcgatac acgacaccac ccacgcgccg cgtcagggat ttagcctgcg
     4261 ccgtctgttt agctacagcc gccgcgaagc actggaattg cgacgcgatc cggtccgttc
     4321 gacgctggcg ctgatgggaa cagtgatcct gatgctgata atgggttacg gcatcagtat
     4381 ggatgtggaa aacctgcgct ttgcggtgct cgaccgcgac cagaccgtca gtagccaggc
     4441 gtggacgctc aatctctccg gttcccgtta ctttatcgaa cagcggccgc tcaccagtta
     4501 tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg gtggcgattg agatcccgcc
     4561 caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa ctcggtgtct ggatcgacgg
     4621 agcgatgccg agccgtgccg aaacggtaaa aggttacgtg caggcaatgc accagagctg
     4681 gctacaggat gtggcgagcc gacaatccac aaccgtcagc caaagcgggc tgatgaatat
     4741 tgagacgcgc tatcgctata acccggacgt aaaaagcctg ccagcgattg ttccggcggt
     4801 gatcccgctt ctgctgatga tgatcccgtc aatgctaagc gccctaagcg tggtgcggga
     4861 aaaagagctt gggtcgatta tcaaccttta cgtgaccccc accacgcgta gtgaattttt
     4921 gcttggtaaa cagttgccat acatcgcgct ggggatgctg aactttttcc tgctctgcgg
     4981 cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt ttcctgacgc tcaccctggc
     5041 ggcgctgctg tatatcatca ttgccaccgg aatggggctg ctgatctcca cctttatgaa
     5101 aagccagatt gccgccattt tcggaacggc gattatcacg ttgatcccgg cgacacagtt
     5161 ttccgggatg atcgatccgg tagcttcgct ggaagggcct ggacgttgga tcggcgaggt
     5221 ttacccgacc agtcattttc tgactatcgc ccgcgggacg ttctcgaagg cgctggatct
     5281 gactgatttg tggcaacttt ttatcccgtt gctgatagcc atcccgctgg tgatgggctt
     5341 aagtatcctg ctgctgaaaa aacaggaggg atgatgcgcc atttacgcaa tatttttaat
     5401 ctgggtatca aagagttgcg cagtctgctc ggtgataaag cgatgctgac gctgattgtc
     5461 ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta cgcctggatc gttgaacctc
     5521 gcgccgatcg ccattgccga tatggatcaa tcgcagttat cgaaccggat cgttaacagc
     5581 ttctatcgcc cgtggttttt gccaccggag atgatcaccg ccgatgagat ggatgccgga
     5641 ctggacgccg gacgctatac cttcgcgata aatattccgc ctaattttca gcgtgatgtc
     5701 ctcgccggac gccagccaga tattcaggtg aacgtcgatg ccacgcgcat gagccaggca
     5761 tttaccggca atgggtatat ccagaatatt atcaacggtg aagtgaacag ctttgtcgcg
     5821 cgctaccgtg ataacagcga accgttggta tcgctggaaa cccggatgcg ctttaacccg
     5881 aacctcgatc ccgcgtggtt tggcggggtg atggcgatca tcaacaacat taccatgctg
     5941 gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg aacacggcac ggtggaacac
     6001 ttactggtga tgccgataac gccgtttgag atcatgatgg cgaagatctg gtcgatgggg
     6061 ctggtggtgc tggtggtatc gggattatcg ctggtgctga tggtgaaagg tgtactgggc
     6121 ataccgattg aaggctcgat cccgctgttt atgctgggcg tggcgctcag tctgtttgcc
     6181 accacgtcaa tcggcatttt tatggggacg atagcgcgtt caatgccgca actggggctg
     6241 ctggtgattc tggtgctgct gccgctgcaa atgctttccg gtggatccac gccgcgcgaa
     6301 agtatgccgc agatggtgca ggacattatg ctgaccatgc cgacgacaca ctttgttagc
     6361 ctcgcgcagg ccattctcta ccggggtgcc ggattcgaaa tcgtctggcc gcagttcctg
     6421 acgctgatgg caattggcgg cgcgtttttc accattgcgc tgctgcgatt caggaagacg
     6481 attgggacaa tggcgtaagg aagggaaact tgacttcata ctgtgatgtc atactattga
     6541 tgtcataaag tgaagtcata aggacatgcc atgagaacca aggtacaggc tttacggaag
     6601 aaacaaaaaa atacattgga tcaaatattt aaaactcctg ttcctcaagg gattaagtgg
     6661 tctgatatag agtcactggt taaagcatta ggcggagaaa ttaaggaagg aagaggttcg
     6721 cgttgtaagt tcatactaaa tatgagcgtt gcgtgtttcc atcggcctca tccgtcgcca
     6781 gataccgata aaggcgctgt agaaagcgtg cgtgactggt tactaagtat aggggtaaaa
     6841 ccatgatcaa actgaagacg ccaaactcta tggagattgc cgggcaacct gcagttatca
     6901 cttatgtgcc agaactgaat gcatttcgtg gcaaattcct gggcttgtca ggttattgtg
     6961 attttgtctc tgacagtatt caggggctgc aaaaagaagg agagctttcc ctgctagagt
     7021 atcttgagga ttgtaaagct gcgggtattg agccgtatgc gcgtacagaa aagattaaga
     7081 cctttacgtt acgctatcca gaatcgctga gtgaacgcct taataatgcg gctgcgcagc
     7141 aacaagtctc tgttaatacc tatattattg aaacactgaa tgagcgtttg aatcatttat
     7201 aaattttagc gacgtacatt acgcagaata tctgccttta caggatctat ctatgcaact
     7261 gaacttaatt ttataaatta agttcagtta atatcttttc tttcctcgtt ttagaaataa
     7321 ctcaatcttc cttcggcagg cactgcaaat gcccgtgccg cacgccgcgc tgggcgataa
     7381 cgtcatcggc aaaatgctgc acgtcaccca tgtcaccttt caacacggcg atttccagac
     7441 agtcgtcgtg gttgatgtgc acatgtagcg tggcgacgga gaggtcgtgg tgatgatgct
     7501 gggtggaaac aatacggctg gctaagtcgc gtttttcgtg ttcatacaca tacgacagca
     7561 ccgcgaaacc ttgtgtgccg tgctgctggg tagcttcctg cgccagggca ctacgcagaa
     7621 tatcgcggat agcttcggaa cggttgttat aaccacgacg ctggctcagg ctgtccagcg
     7681 tctccagtaa atcgtcatca agcgtgatgg tgactcgttg cattggcgtt aaaccttttc
     7741 tgaggtgcga cggcgcacgg ggaatgcggg taataccgcg ttttgtagca cacgtccggc
     7801 gtcagaggaa aaggttaatt tatctcccac cacctgggtt tcgacgattt gtccgttgtc
     7861 catcaccatc acccgctgac agaagcgttc caccaggcgt aagtcgtggg tgatgaacag
     7921 gcaggcggtg ccaaactgtt gttgtagctt tttcagcagg cgaatgacgc ccgcctgtaa
     7981 cacgagatca aggttagaaa cggcttcatc cagaatcagt agtttcggtt cgaccgccag
     8041 cgcgcgagcc aggcagacgc gctggagctg gccgccgctt aactgcggtg ggcgtttgtc
     8101 gagaacgctg tcatcgagat cgaccgcctt cagcatttcg ctggcgcgcg ccagttgttc
     8161 ggattttttc agtgatagca ggtggcgcat cggttcacgc aggatctcgc gcacggtttt
     8221 gcgcggattc acggcgctga tggagtcctg aaataccatc tgaatatcgc ggcggaacgc
     8281 tttacgctgg gcgcgattga gtttcgccag cggttcgccg cgccagctaa tattcccttg
     8341 tgagggagac tctaagccta ccagcagccg cgcgagggtg ctttttccgc agccgctgcg
     8401 tcccagcagg gcgacggttt cgccgctttt cagggtcagg gaaacgttat tcagcaccgc
     8461 ctgatgttga tgttttccgc taaatccacc gtgggcatag tgatgggaaa ggccgcagac
     8521 gttaagtaag gtcatgatgc cagctccata ccgtagaggg cgagatgagc ggaaaccagg
     8581 ctgcgcgtca ccgtatgttt gggggcgtta aacagcgttt ctacatcgcc ctgttcgaca
     8641 attttacctt gtgacatcac cgccacgtca tccgccagac gcgccacaac gcccatatca
     8701 tgggtgacca gcagcattcc cggcgcttgt ttttgcataa tgctttccag cagatcgagg
     8761 atgcgcgcct gtgctaccac gtcgaggtcg gtggtcggtt catcggcgat gataaacggt
     8821 gattcacaca gcaccgccat cgcaatcatc atgcgctgca acatgccgcc gctcatctcg
     8881 aacgggtaca gcttcagcac gcgcgcggcg ttttccagcc ccaccgcttc tatggcagcg
     8941 gtaagcgtag cgtcatcggc gggtttccct aacgccaggc aggtttcccg cgcgtgggtg
     9001 tgcatggtgt gcagtggatt aaaggcgctg cgcgggttct gcatgatggt ggcaattttg
     9061 atgccgcgca gggcgcaagg cgaaaccggt ttgccatcgg ctaaaatttc ccccgccgtc
     9121 tggcgaacgc cagcgggcaa aatgcccagc gtcgcggcgc aggttaatga tttcccgctg
     9181 ccgctaccgc cgactaacgc cagcacgcgc ccgcgttgca gggttaacga tacaccgtgt
     9241 accagcggct gcgcggcctg tagcgcgata ttacgtagtt caatctgttg cggcattagt
     9301 gtgcgtgctc cgtcaccaga tgagggtcca gatgatcgcg cagtgcgtca cccaccaggt
     9361 taaaggccat cacgctgata aacagcgcca gccccggcca gaacatttgc agcggctggg
     9421 tccagatata ctggcgcgcg tcgttaatca tcacgcccca ttcggcggtc ggcgcggtca
     9481 caccgaggcc aaggaaagac atccccgcga cgtgcagcat catatggccg atatccagcg
     9541 ttgccagcac cagcagcgaa gggatcaccg cgcctgccag atgatcgaca aacacccgca
     9601 catggcccgc gccagaaagc cgtgacgcca gcacaaactc gcgttggcgt agtgaaatca
     9661 ccaggctgcg caccatgcgt gcataccacg cccagtgcga cagggcgatg gcgataatta
     9721 cgttggtcag cccggtgccg agcacgccaa ccataaagaa cgacagaatc gaggtcggga
     9781 aggtcataaa catatcggcg acgcgcatgg tggcctgatc aacgcgcccg ccaatcaacc
     9841 cggcgctgcc gccaataacc agccctaatg tcagtaccag cagcaggcag gccattaccg
     9901 aaccgagcga cacgcgggtc gctgccatca gccgcgagaa aatatcgcga cctaagtgat
     9961 cggtgcccag ccagtgctgc gcatccggcg aaagcaggcg cgacggcaaa tcaatcgcct
    10021 gggggtcata cggcagccac cactggctgg tgagtgcaat cagcgccagc agggcgataa
    10081 tgatcagcgc caggcgtacc gaccagcggg aagagaggaa aaagttcacg cgtgcgctcc
    10141 ttcatgacga cgaatgcgcg ggtccagcgc ggcgttgagc aaatcgacaa tcaaattaca
    10201 gaccacaaaa accaccacca tcatcagcgt aaagcactga atcaccggat agtcacggtt
    10261 aaaaatcgcc gacaccgcat agcgcccgac gccaggccag gcaaagatgt tttcgataat
    10321 catcgtcccg ccaatcaatt cgccaatgtg catccccacg gcggtgatca tcggcagcga
    10381 ggcattgcgc agaatatggc ggcgttcggt ctgtttgtca ttcaggccgc gtagccgcgc
    10441 ccaggtgacg tgacgctgac cggcgacgtc cagcatactg gcgcgcagca gtcgcgcgtt
    10501 aatcgccagc gacataaagg cgatggaaac cgcaggcaaa atgatgtgct gccagccgcc
    10561 gtagcccatc gcgggtagcc attgcagata caccgaaaac gccatcacta gcaaaaatgc
    10621 cagccagaag ttgggcatcg acacgccaag aaacgcgata aagcgcacgg cgaaatccgg
    10681 caaacgatcg cggtggcgcg ccgcccagat accgagcggt acggaagtga gcagaattaa
    10741 taccagcgcc gcacctgcaa gttccagcgt ggcgggcagg aagttcagca tatcgtccag
    10801 taccgggcgt tggctggcga atgagatacc aaagtcaaga tgcagcgcct tccacaacca
    10861 ggtaccgtac tggacgtaca gcggctgatc cagcccgagc atggtgcggg tagaggccag
    10921 catctccggc gtcggcggca ggttagataa acgcagataa tcgagcgccg ggtcgccggt
    10981 accgaggcgc agcatcagaa aaatgatcac cgaggcggcg agcaccatcg ggatcagcag
    11041 cagaaagcgg cgtaatacgt aacgcaacat taaggtttca ccggtttaat ctgttcgaac
    11101 ggaatttcgg tggcgatcgg cgcgtagggg atattaccca gctccggttt tgataccacc
    11161 atcatcgaga tgtagctgat aggcagataa accgcctcgt catgcagacg ggtcagaatg
    11221 tcgcgataca gcgcctgacg ttgcgtttcg tcatgggtcg ccagcacttc gccgatctct
    11281 ttatcaatca gcggcttgtc ggctaatcct tgctgtgcct ggaagtcagc gtgcgacggt
    11341 acgcgcattg aactcaggaa ggcgtgtgga tcgtatggcg cgccccaggt gcggtggaaa
    11401 atcatgccaa aacgaccgtc gcgctgacga gcatagatac tgctctcttc ttcgccaatc
    11461 agcgagacat ctgcgccaat ctggcgcata tcagcctgaa tgatttccgc catcgatttg
    11521 cttaacgcat ctgtgccgat gaacgacagt tcaatgcgca gcggctgacc atttttctcg
    11581 cggatgtctt tgcccgcagg cagcgtccaa ccggcttttt ccagcaactc tttcgctttc
    11641 tgcggatcgt actggcgcgg tttcaggccg agattagcgt aaggcacaga aggggcaaac
    11701 agggtgtcgg cgacctgctg ggtgccatat aacgcgttat caatcagcga ttttttgttt
    11761 accgcgtaat taagagcttc acgtactgcc agctcattgg tgggggcttt ggtggtatta
    11821 agcgccagca tcacggtttc gatcggctgt gacagttggg tatggtaagc cgggttctgg
    11881 ctaaagcggg cgaaggtatc gagcggtaat aacccttcgt ttccgtacag caggtcgata
    11941 tcgccagttt caaacgccac cgcgcgggta gtcgggtccg ggatgacgtt aaaggtgatc
    12001 tttttaatcg ctggcttttc gccccaatag ttttcgttac ggacgaagac atcgtactga
    12061 ttcagtttcg attcctgcaa aatccatggt ccggtgccaa tcggcgcttt aattccgttc
    12121 atggtttcat ggtttttaaa ctgcgaggga gcgataaaac ggaaaggacg gggcagggcc
    12181 agttcttgca ggaaaggata gtaggcgctt ttcagggtaa tttgcagctc tgttttactg
    12241 agtgctttaa catcaacaat ctggtttgcc agctccagcc aggcgtgacg ttgacggtta
    12301 tcgagcactg cgcggaagtt ttctgccgcc gcctcggcat cgaacggttc accgttggag
    12361 aatttcacgt catcacgcag ggtgaaggtc caggttttac catcttctga atgagtccag
    12421 ctttttgcca gccacgggat caccgaaccg tctgcctgat acttcaccaa tggttcataa
    12481 accatgctct gggcgaacat ctggttaggc gtgtaaaggt gcgggtttag tggcccgaca
    12541 ttcaccggcc aggcggtagt gatttcatct ggtgcagcgg catggacgat aaaagacgca
    12601 caagccagca gcgcaaatag agtgcggcgg agtgtggaga gcatgataac cccaatggat
    12661 taaaatagat ggcggaaata agtatgacga ttttaagtat tcgtcatact gattacctgt
    12721 tctggatcaa tagatgggca gtcgggtggg cggatcagtt aactgaatcg atccattgca
    12781 cactgtcggc ggtgagcgta aagggggtgg gggtgcagat cgcaaggctt aaattttcta
    12841 gctgacaatg gctgactgac agcgaggagt gataggtgct atctacgctg acgatttgcc
    12901 aggcgctgcc gccacgctgt ttaacgatgg cttctttgcg cgtccagatg cgccagaaca
    12961 tttccagttg ctgatcagga tgcacggcgt ccatttcagc gtgttccccg aggctgaaca
    13021 cggcgttcgc cagccagcgc cagttggcgc gcgggcgaat cacttcgata tcgcagccga
    13081 cttcgccttc atcgctcaac agcagagcga tatcgtcacc gctatggctt aagttgaacc
    13141 atagcggcgt ttccggcgcg aatgcaggtt tgccttgttc gccatagatg atctccggta
    13201 gcggggaaag cgtgtgcgaa agcaatgcac gccccgccag ccagcgttcg cgtcgtggac
    13261 cttgcggtgc ttgctcgcgt aaacccggtg gcagtggagc tgcgcttaag gtcgaaactt
    13321 tccccagaac tatccgatac atatcagggc caacgtttaa tggaaaatga aagtgcgtat
    13381 cgtatcactt gtcgcctcat cccggtaacc gacttttcgg tctgcccggc cccagtaaaa
    13441 tcgccagttt gctgccacct ttcgtcgttt ccatccagat tttacacacg ctggttaatg
    13501 gcaccgaaag tagcatccct accgggccga gcagccatcc ccagattaac aatgaaagaa
    13561 ataccaccat ggtggacatc cccaggcgat ggcccatcat ccgtggttct aaaatattgc
    13621 cgatgaccat atggaccact aaaaacaatg cgccgaccag aatacattcg taaacaccat
    13681 taaacagcag cacctgaatc attggcggta cggcggaaat taccgcgccg atattgggca
    13741 cgtagttgag caaaaacgcc agtactgccc acatcagcgc aaactgcacc cccatcagct
    13801 ccagccccag ccagacgatg acacctgtcc ataaactgag tagcgtcttc aatgcaagat
    13861 agtgcgaaac gcctttaagt gcgcggtgta atcctgcgat gtgaatctgt gggttattca
    13921 gcgcaaaacg cattttgtaa gggacgtggc gcacttcaaa cagcataaaa actacggtca
    13981 tcaccagcaa aagcacgctc gccatcgccc cggaaagccc ggtcattagc gctgtggtga
    14041 aggtaaccac tttttccgag tccatccgct gcagcattcg ctccggcgac atatgcaaat
    14101 taagaaaagg caacatctcc tgcaatttaa aaagtttgcg cgtcagctcc ttattaaact
    14161 tcggcagcat agagataaat tcgttaaacg atgccgccag tacgccgacc agcgcagtta
    14221 gtgcgatcag catcaccacc accacaatcg taatggcaat ggggcgttgt actccccgac
    14281 gaataaacca ggtaacgagc gggttgagga cgatggcaaa aaacagcgcc agtaacagct
    14341 gcacaatgat atctgccgct gcgtgaatgc ccgcgaggat cactaccagc gaggccaact
    14401 tgagcagaat gtgcatgccc gttttatcgg gttgaggggt ttccattggg gcttccttgt
    14461 gactttttgt attaagtgta gcgggagtca cgccagcaat attctgattc ttactgctga
    14521 ttttcacgtc agcacgtggt aaaaatgaaa cactgttgta aaaatgtggt gatcctcatg
    14581 cccgaacccg tagccgaacc cgcgctaaac ggattgcgcc tgaatttgcg cattgtctct
    14641 atagtcatgt ttaacttcgc cagctacctc accatcgggt tgccgctcgc tgtattaccg
    14701 ggctatgtcc atgatgtgat gggctttagc gccttctggg caggattggt tatcagcctg
    14761 caatatttcg ccaccttgct gagccgccct catgccggac gttacgccga tttgctggga
    14821 cccaaaaaga ttgtcgtctt cggtttatgc ggctgctttt tgagcggtct ggggtatctg
    14881 acggcaggat taaccgccag tctgcctgtc atcagcctgt tattactttg cctggggcgc
    14941 gtcatccttg ggattgggca aagttttgcc ggaacgggat cgaccctatg gggcgttggc
    15001 gtggttggct cgctgcatat cgggcgggtg atttcgtgga acggcattgt cacttacggg
    15061 gcgatggcga tgggtgcgcc gttaggcgtc gtgttttatc actggggcgg cttgcaggcg
    15121 ttagcgttaa tcattatggg cgtggcgctg gtggccattt tgttggcgat cccgcgtccg
    15181 acggtaaaag ccagtaaagg caaaccgctg ccgtttcgcg cggtgcttgg gcgcgtctgg
    15241 ctgtacggta tggcgctggc actggcttcc gccggatttg gcgtcatcgc cacctttatc
    15301 acgctgtttt atgacgctaa aggttgggac ggtgcggctt tcgcgctgac gctgtttagc
    15361 tgtgcgtttg tcggtacgcg tttgttattc cctaacggca ttaaccgtat cggtggctta
    15421 aacgtagcga tgatttgctt tagcgttgag ataatcggcc tgctactggt tggcgtggcg
    15481 actatgccgt ggatggcgaa aatcggcgtc ttactggcgg gggccgggtt ttcgctggtg
    15541 ttcccggcat tgggtgtagt ggcggtaaaa gcggttccgc agcaaaatca gggggcggcg
    15601 ctggcaactt acaccgtatt tatggattta tcgcttggcg tgactggacc actggctggg
    15661 ctggtgatga gctgggcggg cgtaccggtg atttatctgg cggcggcggg actggtcaca
    15721 atcgcgttat tactgacgtg gcgattaaaa aaacggcctc cggaacacgt ccctgaggcc
    15781 gcctcatcat cttaaaactt actgaataac cagcgtatta atgatgtttt ctgcggtggt
    15841 ctgcgctttt tgctgatcgt cagttggcag agtaatttgc atggtcagca gttgattacc
    15901 cacgttaccc agaataacgg aagagtacgc cgtctggcct ttcgcggaga taatactgtc
    15961 taactgctgc attttgtgac ctttcagctc aatggcttta ttggttacca cttgcagctg
    16021 cggatcgcgg ctacgttgct gatcttccag acgcttcgcc agcaccgcca gatcttcttt
    16081 tggatcatcg cccatgatga cgatgactgc tttctgcccg gtggcgtcgg accagacatg
    16141 catgttattg gcctgcgttc ccagcttacc gctctggtcg gtcatatccg ctggcagcga
    16201 gaaacttaac ttgccatcaa gcaggttgac gggattcccg gtagcgttac tttccgcgac
    16261 cgaaccctgc gccgtagcgt tagtgtcttt atcatcacag gccgcaagcc ccataaccag
    16321 caggccaatt ccgacatatt taaccagatt gcgcattgac ttcttccttt cgataaacgg
    16381 ccataacggc tcattcatcc atcttatcac aactctgata acgaaccttt aactcgcctg
    16441 caaagcgttg atttcggatt tatctgccag tcttttcaac agcatattga gtaatacgcc
    16501 atacattggc aggaagaaaa cgatactgat taacactttg aaacagtaat cgaccagcgc
    16561 gatttccatc cagtgttcag ccataaaggc atccgggcta cgccagaagg caatgaagaa
    16621 aaaggccagc gtgtcgctga cgttaccgaa cagtgtggac gctgtcggtg ccagccacca
    16681 gcggcgactc tgacgcaggc ggttaaaaac gtgcacgtcg aggatctgtc ccagcgcgta
    16741 ggccatgaaa ctggcggtgg cgatacgggc gacaaacagg ttgaagtggg cgagtgcgcc
    16801 gaatccctgc caggaaccca tatagaatag cgacgagatg acgtaggaga tcagcaacgc
    16861 cgggatcatc accgcaaaga tgatacgacg ggccagcggt gcgccaaaaa tacgcacggt
    16921 caggtcggta gcaaggaaaa taaacgggaa gctaaacgcg ccccaggtgg tatggaaacc
    16981 caaaatggag acgggaagct gaaccagata gttactggag gtgatcacca gcagatgaaa
    17041 taacgataac cagaacaacg ccttatagcg ttgagtttgc gagaaaacgt tcatattgta
    17101 cctttttgat taaccattgg ggtgagggaa cccaatacgt acgacacgtc tgttacctta
    17161 acataataac gtgcttaccc ttttttcgag ccgccgcatg atactgcttt acgttgacaa
    17221 tgcaatggtt aattttcacg caatcgttaa cctggtttgc ttacggactc acagggcgta
    17281 aactagcgcc gtttttttat gtgatgagaa gaaaatgacc gatctctttt ccagccctga
    17341 ccacacactc gacgcacttg gcctgcgctg cccggaaccc gtaatgatgg tgcgcaaaac
    17401 agtgcgcaat atgcagcctg gcgaaacgtt gctgattatc gccgacgatc cggccactac
    17461 ccgcgatatt cctgggtttt gtacctttat ggaacacgaa ctggttgcga aagagacgga
    17521 tggactgcct tatcgttatt tgattcgtaa aggcggttga tgggggctga ttggcttcga
    17581 tgccgccttt tcccctcacc ctaaccctct ccccagaggg gcgaggggac cgatcgcgct
    17641 caatgttgcg atcggtttgc cttatctcct gcgcaacaat cttaacgcat tcgctgtcac
    17701 cagcaccgtc gcacccgtat ctgccagcac cgccagccat agtccggtca tccctaaaag
    17761 cgtggtgacg aggaagatcc ctttcagtcc cagcgcaatg gtgatgttct ggcggatatt
    17821 ggcgtgagtg gcgcgtgcca gttcaatcat ttgcaccagg ccgcgcaggt ggttatgggt
    17881 taatgctgca tcagcggttt ccagcgccac gtctgtgcca ctgcccattg caatcccaat
    17941 ggcggcagct ttcatcgctg gcgcgtcgtt aataccgtca ccgaccatcg ccagcggcgc
    18001 atgttgattc agctcggtca ccgctttgac tttatcttcc ggcaataggc ccgctttaaa
    18061 ctccagcccc agttcgccgg caattgccgc cgctgcacgt ggattatcgc cggtgaggat
    18121 cactccttta acccccagcg cgttcagttc actgatggca gttgcagcgt cggcgcgcag
    18181 ggtatcctgc aatgcaatga tacccagcac gtcatcatta cgtactacca gcacaaccgt
    18241 ttgcccggcg ctttccagtt cattaatcaa accagcaaat gcatcagcgg gatgtttccc
    18301 ggcagcgcaa atcaatacgc gctcaccgtt aacctgcgct tcaatgccag acccgaccag
    18361 cgcccgctgt gattcggcgg tgggaatggc gagttcagca acctgtgctt cgcgtacgat
    18421 ggcttgcgcc agtggatgcg tcgcgccttg ctcgaccgcc gccgccagtg tcagcagttc
    18481 agattcacta atacccgttg ccggatgaat cgcggtaacg cgcggtttac cgacggtcag
    18541 cgtaccggtt ttatcaaacg ccacctgggt aacacgaccc agctgctcca gcgccgctcc
    18601 gcctttaatc aacgccccac gacgcgctgc cgccgccagc ccggaggtaa tcgccgcagg
    18661 cgtggagata actaacgcac acgggcagcc aatcagcagc agcgtcagcc ctttataaat
    18721 ccactcctgc cagctggcgg caaacagcag cggtggcacc agcgtcacca gcagggcgac
    18781 ggccataatc gctggcgtat aaatacggct gaaacggtcg ataaaccgct caatgggggc
    18841 gcggcgctct tcggcttctt cgatcagttt cagaatccgg tcaatggcgc tggcacccgg
    18901 ttctgacagc acttccagcg ttaccagacg gtctacgctg gtggctcctg caggaacttt
    18961 atcgcccgtc gcgcgctcca ccggaatgga ttctccggtc agggcgcttt catcaaaact
    19021 ggcaaacggt gagagcagtt taccgtcggc aggcaaacgc ccacctgcgg cgacttcaat
    19081 cacatcgcca ggccgcaggc tgttaatcgc cacctcttcc cgctcaccgt tacgcaggcg
    19141 cgtggcggtt tctggtttca gcgccattaa cgcgctaacg ccctgacgcg cgcggctggc
    19201 ggcccagcct tccagtcgtt caccaatcaa aaacagcagc aacaccatcg cagcttcagc
    19261 cgttgcgcca ataaacagtg caccaatagc ggctacgctc attaaggttt caatggcgaa
    19321 gtagctgccg gatttgatca gccgtaatgc ctgacgagca atcgggtaca gcccagccag
    19381 cgtggtcgcg ataaacgcca gttgcccgaa cggatgatta aattgctcca aactccagct
    19441 gattgccatc atcacgatta gcgtaatcag cggcagattc tctttcaggc gcgatgcttg
    19501 tggctcgtcg gcggcctgct catcgcgcag ggaatagccc gctttttgca ccgcagattc
    19561 aacctgggca cggatgtcat tgtcggcatc aaccaccagt ttttcggtgg cgaacaacac
    19621 ctgcacctga ttcacgcctg caagctggcg cacggcattt tctaccttgc gcgcacaggc
    19681 ggcgcagtcc atgccgctga ctttccagct atagcgggtg ccggagacgt tttcagagag
    19741 cgttggcgag ctggaacatg cgccgtcgca gcaacagtcg ttggcgttct gtaccgtggt
    19801 tagcggtttg aacgcagcaa attgaggggc tttcttgccg tgattgtcag gagtcgacat
    19861 ggcatcctcc agttaagttt tttctcatta accgaaggat acactctgga gtcgactcca
    19921 gagtcaagtt ttatcagaga tacagtgagc ggacgatgag gaagtgaccg gcaaagtagc
    19981 aggccgcagc aatcgcgtta tccgcacgga agcgccggcg atagtggcta cccagccaga
    20041 caaagttact gataaacagc agcgaagcgc cgacaaacgc agagagtgcc ggagcggtcg
    20101 gacggaagaa ccacagttca cctgccagcc acaccatcac cagcgtcatg ccgataaacg
    20161 tgcagatagg ccaacggtac tcttccagcc gcgtccagat aatcgccagt aacagcgcac
    20221 ccagaaccag caacaccagt ggcagaggcc agaagaaaga gagcgtcatc tgactggcga
    20281 aatagatggt gtacagcagg tgcgagagga aaaacgcgcc gatggcgtac atcagacgtt
    20341 gacgtggcaa cagggttagc gcatcgccca gcagtgaggc gcacagccct gccagcacca
    20401 gatagctaat ggcgtcgaac atcggcgctt gccaggccag taacagcagg agaagaaggg
    20461 ttaacggttt aaacacccag cgttgccagg ttggcccacg atacgatgca tccacagata
    20521 gccatgcgga aagacagaca gcgataaacg accaaagcat cttagttcct tgaattcgtt
    20581 attttttcgt aagcggaatg cctacggtct tgcttccagt ttagtggcga gggtggacgg
    20641 atgacaacac cataactggc agggtatgct tatttccgca ttttccgatg agggataaag
    20701 atgagcaaac caactctttt ctttattgtt atcattggct taattgtcgt cgccgcatcg
    20761 tttcgtttta tgcaacagcg acgggaaaaa gctgataatg atatggctcc gctccagcaa
    20821 aagctggtgg tggtgagcaa caagcgggaa aaaccgatta acgatcgccg ttcgcgccag
    20881 caggaagtga ctccggcagg caccagtatg cgctatgagg caagcttcaa accgcaaagc
    20941 ggaggaatgg agcagacgtt tcgcctcgac gcccagcagt accatgccct gacagtgggc
    21001 gataaaggta cgctgagcta taaaggaacg cgctttgtta gctttgttgg cgaacaataa
    21061 ggttattttt taactttaaa tttcttctgc cagaccagca gttcaaacac gccgaaaagg
    21121 aaaatccgca ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa agtggatttc
    21181 aatagtgcca gctgcatacc atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa
    21241 atattcagcg ggcgcgggaa gggatgcacc agattgagga ttaaaaatcc ccaaacgcag
    21301 agcattaaca aacgtccaat attaatcagc atcactttct ccttgtgctt cgcgttgata
    21361 cagccgataa gccacctgac ccgccacttt ttcccgatgt aatgaccagt ttgccggaac
    21421 agtgggcaga ccgttttcga cttcgctttc gacataaatc agggcttcgt cagccagcca
    21481 gccgttatct tccagtaaat ttatcgtctc ttctaacaag ccacggcgga acggtggatc
    21541 gacaaacacg atattatgcg gtgtaccttt ttgcgccagg aatgacatcg cgttgctgtt
    21601 caccacgcgt gcattgcctg cttttagtgt cgccagattc ttaattaact gctgagaaac
    21661 cgcgcgatcc atctcaatca acgtggcccc cgcagcgtag cgcgataacg cttccagtcc
    21721 cagcgcgccg ctcccggcga agcaatccag acattgggcg tcaacaatga ccggagccag
    21781 ccagttaaac aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg
    21841 aaccgggagt ttacggcctc gccactgccc gccaataatg cggatttggc cgctgccgga
    21901 atgattcggt tttttcatga taaattgctc aatccgccag ataacacata cttgcaggcg
    21961 gtgatgtgaa ttaagttaag tgatagacta tttcatcatt tttttagctg ctatgtacat
    22021 agcgttaacg ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa
    22081 aaaaacgtgg ctttttttcc tggctgggct ttggtcaaaa agagcagacc ccggaaaaag
    22141 agacagaagt tcagaatgaa caaccggttg tagaagaaat cgttcaggcg caagagcctg
    22201 cgaaggcttc tgaacaagcc gtcgaagagc aaccgcaggc gcatactgaa gccgaggcgg
    22261 aaacttttgc tgccgacgtt gtggaagtca ctgaacaggt tgctgaaagt gaaaaagcgc
    22321 agcctgaagc ggaagtcgtt gcacagccgg aaccggtcgt agaagaaacg gcggagccag
    22381 tggctatcga acgtgaagag ctgccgttgc cggaagacgt caacgccgaa gaggtttcgc
    22441 cagaagagtg gcaggctgaa gcggaaaccg tagagattgt cgaagcggcg gaagaagaag
    22501 cggctaaaga agaaatcacc gacgaagagc tggaagcaca ggcgctggct gccgaagcgg
    22561 cagaagaggc ggtgatggtg gttcctccgg tagaagagca gccggtggaa gaaatcgctc
    22621 aggagcagga aaaaccgacc aaagaaggtt tcttcgcgcg cctgaaacgc agcctgttaa
    22681 aaaccaaaga aaatctcggt tccggattta tcagcctgtt ccgcggtaaa aaaatcgacg
    22741 atgatctgtt tgaggagctg gaagaacagc tattgatcgc cgatgtgggc gtggaaacca
    22801 cacgtaaaat tatcaccaat ctgacggaag gcgcatcccg caagcagctt cgtgatgccg
    22861 aggcgctcta tggcctgctg aaagaagaga tgggcgagat tctggcgaaa gtcgatgagc
    22921 cgctgaatgt tgaaggtaaa acgccgttcg tgatcctgat ggtgggcgtc aacggtgtgg
    22981 gtaaaaccac gacgattggt aagctggcgc gtcagtttga gcagcagggt aaatcggtga
    23041 tgctggcggc gggcgatact ttccgtgcag cagcggttga acagcttcag gtctggggtc
    23101 agcgcaacaa tattccggtg attgcccagc ataccggtgc ggattccgcc tctgttatct
    23161 tcgacgccat tcaggcagct aaagcgcgta atatcgacgt cctgattgcc gataccgccg
    23221 gacgtctgca gaacaaatcg catctgatgg aagagttgaa gaaaatcgtc cgcgtgatga
    23281 agaaactcga cgttgaagcg ccgcatgaag ttatgctgac tattgatgcc agcaccgggc
    23341 agaacgcggt aagccaggcc aaactgttcc acgaagctgt tggcttaacc ggcatcacgc
    23401 taacgaaact ggacggcacg gcgaaaggcg gggtaatttt ctcggtggct gaccagtttg
    23461 gtatccctat ccgctacatt ggtgtcggcg aacgtattga ggatttgcgt ccgtttaagg
    23521 cggacgactt tatagaggca ctttttgccc gagaggatta acaatgattc gctttgaaca
    23581 tgtcagcaag gcttatctcg gtgggagaca ggcgctgcag ggcgttacgt tccatatgca
    23641 gccgggtgag atggcgtttc tgaccggtca ttccggcgca gggaaaagta ccctcctgaa
    23701 gctgatctgt gggattgagc ggcccagcgc cgggaaaatc tggtttagcg gccatgacat
    23761 cacgcgtctg aaaaaccgtg aagttccgtt tctgcgccgc cagattggca tgattttcca
    23821 ggatcaccat ctactgatgg accgtactgt ctacgataac gtggcgatcc cgctgattat
    23881 cgctggtgcc agcggtgacg atattcgtcg ccgggtgtcg gcggcactgg ataaagtcgg
    23941 gctactggac aaagcgaaga acttccctat tcagctttcc ggcggtgaac aacagcgtgt
    24001 tggcattgcc cgcgcggtgg tgaataagcc cgcggtactg ctggcggacg aaccgaccgg
    24061 taacctggac gacgcgttgt cggaagggat tctacgtctg tttgaagagt ttaaccgcgt
    24121 tggggtaacc gtattgatgg caacgcacga catcaacctg atttcacggc gttcctatcg
    24181 catgctcacc ctgagcgatg gtcacttgca tggaggcgtg ggccatgaat aagcgcgatg
    24241 caatcaatca tattcggcag tttggtgggc gtctggatcg cttccgtaaa tcggtcggcg
    24301 gctcaggcga cggcggtcgt aacgcaccga aacgcgcgaa atcctcgcca aaaccggtaa
    24361 atcgcaaaac caacgttttc aacgaacagg tgcgctatgc cttccacggc gcattgcagg
    24421 atctgaaaag caaaccgttc gccacgtttt taacggtgat ggttatcgcc atctccctga
    24481 cgctgcccag cgtctgttat atggtgtaca aaaacgttaa tcaggcggcg acgcagtatt
    24541 atccgtcacc gcaaatcact gtttacttgc aaaaaacgct ggacgatgac gccgctgcgg
    24601 gcgtggtggc acagttgcag gccgagcaag gcgtggagaa agtgaactat ctttctcgtg
    24661 aagacgcact gggcgagttc cgtaactggt ctggttttgg tggtgcgctg gatatgctgg
    24721 aagaaaaccc gcttccggca gtggcggtgg tgatcccgaa actcgatttt caggggacgg
    24781 aatcactcaa tacgctgcgt gatcgtatca cgcagattaa cggcattgac gaagtgcgga
    24841 tggatgacag ctggtttgcc cgtctggcgg cgttgaccgg gctggtcggg cgcgtttcgg
    24901 cgatgatcgg cgtgttgatg gtggcggcag tgttcctcgt catcggcaac agcgtacgtc
    24961 tgagcatctt tgctcgccgt gactccatca acgtgcagaa actgattggt gcgacggatg
    25021 gtttcatcct tcgcccgttc ctgtatggtg gcgcactgct gggattttct ggtgcattgt
    25081 tgtcattaat tttgtcagaa attctggtgc tgcgattgtc atcggcggtt gcggaagtgg
    25141 cacaggtttt cggaacgaag tttgatatca atggcttatc attcgatgaa tgcctgctat
    25201 tgctgctggt atgctcgatg attggctggg tggcagcgtg gcttgccacg gtacaacatt
    25261 tacgccactt tacgcctgaa taataaaagc gtgttatact ctttccctgc aatgggttcc
    25321 gtagcaggga aagagacccc gttgtctctt cccggtattt catctctatg tcacattttg
    25381 tgcgtaattt attcacaagc ttgcattgaa cttgtggata aaatcacggt ctgataaaac
    25441 agtgaatgat aacctcgttg ctcttaagct ctggcacagt tgttgctacc actgaagcgc
    25501 cagaagatat cgattgagag gatttgaatg actgacaaaa tgcaaagttt agctttagcc
    25561 ccagttggca acctggattc ctacatccgg gcagctaacg cgtggccgat gttgtcggct
    25621 gacgaggagc gggcgctggc tgaaaagctg cattaccatg gcgatctgga agcagctaaa
    25681 acgctgatcc tgtctcacct gcggtttgtt gttcatattg ctcgtaatta tgcgggctat
    25741 ggcctgccac aggcggattt gattcaggaa ggtaacatcg gcctgatgaa agcagtgcgc
    25801 cgtttcaacc cggaagtggg tgtgcgcctg gtctccttcg ccgttcactg gatcaaagca
    25861 gagatccacg aatacgttct gcgtaactgg cgtatcgtca aagttgcgac caccaaagcg
    25921 cagcgcaaac tgttcttcaa cctgcgtaaa accaagcagc gtctgggctg gtttaaccag
    25981 gatgaagtcg aaatggtggc ccgtgaactg ggcgtaacca gcaaagacgt tcgtgagatg
    26041 gaatcacgta tggcggcaca ggacatgacc tttgacctgt cttccgacga cgattccgac
    26101 agccagccga tggcaccggt gctctatctg caggataaat catctaactt tgccgacggc
    26161 atcgaagatg ataactggga agagcaggcg gcaaaccgtc tgaccgacgc gatgcagggt
    26221 ctggacgaac gcagccagga catcatccgt gcgcgctggc tggacgaaga caacaagtcc
    26281 acgttgcagg aactggctga ccgttacggt gtttccgctg aacgtgtgcg ccaactggaa
    26341 aagaacgcga tgaaaaaatt gcgtgctgct atagaagcgt aatttccgct attaagcaga
    26401 gaaccctgga tgcgagtccg gggtttttgt tttttgggcc tctgtaataa tcaatttccc
    26461 ctccggcaaa acgccaatcc ccacgcagat tgttaataaa ctgtcaaaat agctattcca
    26521 atatcataaa aatcgggtat gttttagcag agtatgctgc taaagcacgg gtagtcatgc
    26581 ataaaacgaa ataaagtgct gaacaacaac atcacaacac acgtaataac cagaagaatg
    26641 gggattctca ggatgaacat aaagggtaaa gcgttactgg caggatgtat cgcgctggca
    26701 ttcagcaata tggctctggc agaagatatt aaagtcgcgg tcgtgggcgc aatgtccggt
    26761 ccggtggccc agtacggtga ccaggagttt accggcgcag agcaggcggt tgcggatatc
    26821 aacgctaaag gcggcattaa aggcaacaaa ctgcaaatcg taaaatatga cgatgcctgt
    26881 gacccgaaac aggcggttgc ggtggcgaac aaagtcgtta acgacggcat taaatacgtg
    26941 attggtcacc tctgttcttc atcaacgcag cctgcgtctg atatctacga agacgaaggc
    27001 attttaatga tcaccccagc ggcaaccgcg ccggagctga ccgcccgtgg ctatcagctg
    27061 atcctgcgca ccaccggcct ggactccgac cagggaccga cggcggcgaa atatattctt
    27121 gagaaggtga aaccgcagcg tattgctatc gttcacgaca aacagcaata cggcgaaggg
    27181 ctggcgcgtg cagtgcagga cggcctgaag aaaggcaatg ctaacgtggt gttctttgat
    27241 ggtatcaccg ccggggaaaa agatttctca acgctggtgg cgcgtctgaa aaaagagaat
    27301 atcgacttcg tttactacgg cggttatcac ccggaaatgg ggcaaatcct gcgtcaggca
    27361 cgcgcggcag ggctgaaaac tcagtttatg gggccggaag gtgtggctaa cgtttcgctg
    27421 tctaacattg cgggcgaatc agcggaaggg ctgctggtga ccaagccgaa gaactacgat
    27481 caggttccgg cgaacaaacc cattgttgat gcgatcaaag cgaaaaaaca ggacccaagc
    27541 ggcgcattcg tttggaccac ctacgccgcg ctgcaatctt tgcaggcggg cctgaatcag
    27601 tctgacgatc cggctgaaat cgccaaatac ctgaaagcga actccgtgga taccgtaatg
    27661 ggaccgctga cctgggatga gaaaggcgat ctgaaaggct ttgagttcgg tgtgtttgac
    27721 tggcatgcca acggcacggc gaccgatgcg aagtaatcat taatcggcaa ctttgggttg
    27781 ccgccaaatt gctaatatcg agtacgttgc ttcatgccgg atgcggcgta aacgccttat
    27841 ccggcctaca agatccaaag aaatcagtaa attgcaacac acattgtagg cctgataagc
    27901 gtagcgcatc aggcaatttc tcgttggtca tcacttttga aatcaacttg acgattaacg
    27961 cttctcccag ccgccctgtt gcgccgtaaa tcccagtgcc tgcataaacg ccgtcatcac
    28021 accgcgatct tccacgcctg catccgccat ccaccagcat gaaacgccag gattgttacg
    28081 caaaacctct tccagcagat attgccccac accgcgacgt cgggtgactt cccgcacacg
    28141 cagggaatcc agtgctccct cggtgccgct taaggttacc cgtacggcag cgagcaggcg
    28201 ctcgttaaaa cgcgcggcgt agatacggtg gttatcgtca acctgtaaag aggaagggga
    28261 atactccggc cagatctttt gcaggtcaat ccggtcctgg tcgctaaagt tttctaatcg
    28321 aatgatggtc agcttcatgg gtaacccgtg taaatcacaa aagtggaacc agtgtagcga
    28381 aataatttaa tcggaggctt tctctttttt atttcttttg gcaggtgatt aattttttaa
    28441 cagcaataat tacaaagtta aa
//
LOCUS       DAJSKJ010000028        28255 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000028 DAJSKJ010000000
VERSION     DAJSKJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.028"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..106)
                     /locus_tag="ON345_001095"
     CDS             complement(<1..106)
                     /locus_tag="ON345_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3071192.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRA"
     gene            114..635
                     /gene="tnpA"
                     /locus_tag="ON345_001096"
                     /pseudo
     CDS             114..635
                     /gene="tnpA"
                     /locus_tag="ON345_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(676..1998)
                     /locus_tag="ON345_001097"
     CDS             complement(676..1998)
                     /locus_tag="ON345_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HCR3071193.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPLQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPRASVAKAILDELLEANKAYWPELR"
     gene            complement(1998..3614)
                     /locus_tag="ON345_001098"
                     /pseudo
     CDS             complement(1998..3614)
                     /locus_tag="ON345_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            3910..4626
                     /locus_tag="ON345_001099"
     CDS             3910..4626
                     /locus_tag="ON345_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3071194.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4623..6284)
                     /locus_tag="ON345_001100"
     CDS             complement(4623..6284)
                     /locus_tag="ON345_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR3071195.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6480..6908)
                     /gene="ibpB"
                     /locus_tag="ON345_001101"
     CDS             complement(6480..6908)
                     /gene="ibpB"
                     /locus_tag="ON345_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR3071196.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7020..7433)
                     /gene="ibpA"
                     /locus_tag="ON345_001102"
     CDS             complement(7020..7433)
                     /gene="ibpA"
                     /locus_tag="ON345_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR3071197.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7427..7499)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7739..8071
                     /locus_tag="ON345_001103"
     CDS             7739..8071
                     /locus_tag="ON345_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR3071198.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8073..9287)
                     /locus_tag="ON345_001104"
     CDS             complement(8073..9287)
                     /locus_tag="ON345_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR3071199.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHERSPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGVKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9388..10452
                     /gene="cbrA"
                     /locus_tag="ON345_001105"
     CDS             9388..10452
                     /gene="cbrA"
                     /locus_tag="ON345_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR3071200.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10449..11786)
                     /locus_tag="ON345_001106"
     CDS             complement(10449..11786)
                     /locus_tag="ON345_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3071201.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11861..13009)
                     /gene="dgoD"
                     /locus_tag="ON345_001107"
     CDS             complement(11861..13009)
                     /gene="dgoD"
                     /locus_tag="ON345_001107"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HCR3071202.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(13006..13623)
                     /gene="dgoA"
                     /locus_tag="ON345_001108"
     CDS             complement(13006..13623)
                     /gene="dgoA"
                     /locus_tag="ON345_001108"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR3071203.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFSVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13607..14485)
                     /gene="dgoK"
                     /locus_tag="ON345_001109"
     CDS             complement(13607..14485)
                     /gene="dgoK"
                     /locus_tag="ON345_001109"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR3071204.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14482..15171)
                     /gene="dgoR"
                     /locus_tag="ON345_001110"
     CDS             complement(14482..15171)
                     /gene="dgoR"
                     /locus_tag="ON345_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR3071205.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15449..16105
                     /locus_tag="ON345_001111"
     CDS             15449..16105
                     /locus_tag="ON345_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071206.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(16151..16963)
                     /gene="yidA"
                     /locus_tag="ON345_001112"
     CDS             complement(16151..16963)
                     /gene="yidA"
                     /locus_tag="ON345_001112"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR3071207.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(17078..17476)
                     /locus_tag="ON345_001113"
     CDS             complement(17078..17476)
                     /locus_tag="ON345_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR3071208.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17716..20130)
                     /gene="gyrB"
                     /locus_tag="ON345_001114"
     CDS             complement(17716..20130)
                     /gene="gyrB"
                     /locus_tag="ON345_001114"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR3071209.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(20159..21232)
                     /gene="recF"
                     /locus_tag="ON345_001115"
     CDS             complement(20159..21232)
                     /gene="recF"
                     /locus_tag="ON345_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR3071210.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(21232..22332)
                     /gene="dnaN"
                     /locus_tag="ON345_001116"
     CDS             complement(21232..22332)
                     /gene="dnaN"
                     /locus_tag="ON345_001116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR3071211.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22337..23740)
                     /gene="dnaA"
                     /locus_tag="ON345_001117"
     CDS             complement(22337..23740)
                     /gene="dnaA"
                     /locus_tag="ON345_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR3071212.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            24037..24117
                     /locus_tag="ON345_001118"
     CDS             24037..24117
                     /locus_tag="ON345_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071213.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            24347..24487
                     /gene="rpmH"
                     /locus_tag="ON345_001119"
     CDS             24347..24487
                     /gene="rpmH"
                     /locus_tag="ON345_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR3071214.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24504..24863
                     /gene="rnpA"
                     /locus_tag="ON345_001120"
     CDS             24504..24863
                     /gene="rnpA"
                     /locus_tag="ON345_001120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR3071215.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24827..25084
                     /gene="yidD"
                     /locus_tag="ON345_001121"
     CDS             24827..25084
                     /gene="yidD"
                     /locus_tag="ON345_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR3071216.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            25087..26733
                     /gene="yidC"
                     /locus_tag="ON345_001122"
     CDS             25087..26733
                     /gene="yidC"
                     /locus_tag="ON345_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3071217.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26839..28203
                     /gene="mnmE"
                     /locus_tag="ON345_001123"
     CDS             26839..28203
                     /gene="mnmE"
                     /locus_tag="ON345_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR3071218.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIVLWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6826 a         7621 c         7206 g         6602 t
ORIGIN      
        1 gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cactcctgtt
       61 gaccacctgg tcttaactgg aatttaaatg cctgtaatcg tttcacaggc caaatgatac
      121 tttggtctat gaataaagaa accgatatcc gacggggaag gcattgtgtt ttcctgatgc
      181 atgttcacct ggtctttgtc accagatacc gacgtcagat ttttgaccat gacgcgacag
      241 aaaaactacg cgcttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa
      301 tggatggcga accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat
      361 ccagtctggt aaacagcctc aaaggcgtat cgggtaggtg actgcgacga gatcgaccag
      421 atattgcagt caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta
      481 gctgcggagg tgcgccaata tccgtcatcc gccaatacgt tgaacaacag caaacacctg
      541 gtcaggtgga aaaccgcgcc ttatatcccc gccctgaagg acggggtttt acggcgcacc
      601 ggataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc acccaacgga
      661 tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct ccagcaactc
      721 atccagaata gctttggcga ccgaggctct cggtaccgtt ttcgacaacg taatcgcctg
      781 ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt ccaccgccac
      841 ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca gtggttctgg
      901 tccgttgtgc ccgaccagac acgggatttc gaccatcgct tcatcatcaa agttatgaat
      961 tgccccgttg ttaggcacaa tcagcaacat ccgctcctga gtgttgaagg caattgccgc
     1021 cgccagatcg acgatgtatg acgcatgttc gtcaatttcc agcttgcccg ccgctgaatt
     1081 tcctgccgcc gtaatggcgc ggcaagcatc gaaaacctgt ttctcgcgat gttccatcac
     1141 ctcattcgcg cgggtatgtt cagggttgga atgctgaacg acataatccg ggaagagata
     1201 atatttcaga taggtgtttg gtaatgtatc cgggtccagt gcctggacat cccgcgcttt
     1261 tgcataggtg tcgttccagc tcgcttcagt atgtgtatct tgcagcggtg gaacataacc
     1321 gtgcttagag acatattgcc gcagctgggg catcaggtcg ttgccctgca aatcttcaat
     1381 tgatgtccac cagccaaagt ggttcaggcc gtagtagcgc acgcgcatct gtttgcgatc
     1441 ttgcagccca acaatttgcg ccatccggct ttcaataccg attggcatgt cacagatgtt
     1501 gaggattttc gcattcgggc gcagacgacg cgtggcttct gcgacaatgg ctgccgggtt
     1561 ggagtagttg agcatccagg catttggtga atatttttcc atataatcca ccagttccag
     1621 gacgccgcca atggaacgca tgccgtacgc tattccgccg ggtccgcaag tttcctgacc
     1681 aacaacgccg tggcgcagcg ggattttttc atccagttcg cgcatcgggt atttgccgac
     1741 gcggatgtgc gccatgacaa aatcaacgtc gctgaatgcc acttcaggat cggtggtgta
     1801 actaaacgca atgtccggcg ctttttcttt aaggatgact ttacaggctt cggcaatcac
     1861 ttcctgccgc gcaccatcgt tatcataaaa tttcagtgca cgaagcggga aacggtcctg
     1921 attcgccagg agcatcaaca cgatgcctgg cgtaaaggtg cttccaccgc ctgcgacaac
     1981 cactgagaat ttggtcatta tactgcctcc gtaatggcaa cattttctgc tgattgatga
     2041 gaattaatta agctatcgag ctgttcacgc agctgggata catgcagacc aatgatcacc
     2101 tgaatggcat cgccactacg gaagacgccg tgcgctccca gctttttaaa gacttcgtca
     2161 tccagcgttt gtgacatgtc atgcagtgca atacgtagac gcgtcgcgca attgttaatg
     2221 ctggagatat tgccgacccc gcccagggct tgcaggatac cggcagcctg atccagctct
     2281 ttttttggct ctgcagcggt ggtttggcct cgcgaggctt tgtattcggc ttttgagtag
     2341 agtttcactt ccgcatcttc acgtcccggc gtgcacatgt tgaactgcag aatcagggtg
     2401 cggaaaacca cgaagtacag cagggtaaag cacaacccaa tggcgatttg ggtcagcatc
     2461 atatccgcgt ggttgctgaa catcgggatc cagttttgcg gtaaaacctg gtcaatcaga
     2521 cctccgccca tgttgcccac cacaccaaag agatacatta cggtcgacat tgaggccgcc
     2581 agcacggcgt gtaccgcaaa cagcaacggt gaaatgaaca ggaaggtaaa ttccagcggt
     2641 tcggtaattc ccaccagcat ggcggttaag gttgcgggaa tcagcaagcc cgccactttt
     2701 acccgatttt ccggtgcggc agtgaagtac atcgctaaag aaatgcccac ggcaccaaag
     2761 atttttgagt taccgtgcag ggcaaaaccg ccttccggga acaacgattt cagcggctcg
     2821 gcgctcagac tgaactcttg cagatgctgc gcccagtaca tctaaatgcc gccttcaaca
     2881 gctgccggac caaagataaa cggtccgtag atgaggtggt gtaaaccggt tgggatcaga
     2941 atacgttcga ggaaggtgta aacccacacc ccaagcgcac ccgccgaacg caggaacgct
     3001 tgcagagatt caatccccat ttgtactttt ggccagccga gcaatgtcag ccaggcacag
     3061 gggatcatca ccaggaaggc gataatcacc acataagacg tcccctggaa aatgccgaga
     3121 aaaaccggca gttttttatc gaacagacgg ttatgcagcg ccgtcacaat gccggaaatg
     3181 ataattgcgc cgataatgct ggtatcgagg gttttaatcc cggccatcat tgtcagaccg
     3241 ctacctgcca ccgcgtcctg agtgaaatcg acgccgaagt agcttcccca ggtcattccc
     3301 atcgcgttga tgaaatagtt ccaggtcagg aaactcacca tcaccgccag acaagcacgt
     3361 ccctgcgctt gcttagcaag gccaatgggt aaaccgacag caaaaatcag cggcatatta
     3421 cggaataccg tccaaccgcc ctcttcaata atgtgtacga tttgcgcgaa taaactgttc
     3481 ggatcggtca gtgattcccc gacaaacatc gggttttgca gcaagatggc aagacccacc
     3541 acaatcccgg cgaagggaaa cagcagcact ggcgtgaaca tcgcgccgcc aaagcgttga
     3601 atttgactga gcatttccac atcctctttt ctcaattctg taagtaggaa gcctgattgg
     3661 ttatgcggca ggcctggagc gaggatagga agatgcgatc ggcagaacat gacgacccaa
     3721 tgggattcgt gatcgctttc atgcttttcg ttgggcggta gagatctacg ttttcgcgaa
     3781 aaagtagaca tctgtgagca ctggtacaca gcatcgaaca catctttaaa aaaaagatgt
     3841 tttttcaatc gattaagcag aacttgtggg cgcattaccc gggcttgcag gcaaaaaaga
     3901 gatctagaga tgatctacaa aagcattgcg gagcggttaa gaattcgact taattccgcg
     3961 gatttcacgc taaacagcct tcttcccggt gaaaaaaagt tggcggaaga atttgcagta
     4021 tcccggatga ccatccgtaa agcgattgac ctgctggtag cgtgggggct ggtggtccgc
     4081 cgccacggca gcggcactta cctggtgcgc aaagatgtgc tgcatcaaac cgccagcctg
     4141 accggactgg tggaggtgtt aaaacggcag ggaaaaacgg tcaccagcca ggtgctgatt
     4201 tttgaaatca tgcctgcgcc tccggccatt gccagccagt tacggattca aatcaacgag
     4261 cagatctact tctcccgtcg cgttcgtttt gtggaaggga aaccgctgat gctggaagac
     4321 agctatatgc cggttaaact gttccgtaat ctttcgctgc aacatctgga agggtcgaag
     4381 tttgaatata ttgaacaaga gtgcgggatt ttgattggcg gtaattatga aagcctgacg
     4441 ccggtgctcg ccgatagact gctggcgcgg caaatgaagg tagcggaaca cacgccactg
     4501 ctgcggatca cctcgttgtc atatagcgag agcggggagt ttttgaatta ttcagtgatg
     4561 ttcagaaatg ccagcgaata ccaggtggag taccatttac ggcgactcca cccggaaaag
     4621 agttaaccga tactccagaa gagcaccgcc agtaattggg gggtgataat tcgcagaaac
     4681 atcactaacg gatagacagt ggcgtaagag agcgccgccg caccactggt tggatgaaga
     4741 ttattagcaa acgccagcgc cggaggatcg gtcatggaac ctgccagcat cccgcacatg
     4801 gtcaggtaat tcattttggc taacatccgc gccagaatgc caacagtaat cagcggaacg
     4861 gcggtgatca gggcaccata accaatccag cttagccctt cgccattgac cagggtattc
     4921 acaaaatccc caccagattt cagaccaacg accgagagga acagcacgat ccccagctcc
     4981 cgcagcgcga ggttggcact tggcggcata aaccagtaca gcttgccgat actgccgata
     5041 cgcccgagga tcaacgccat aatcagcgga ccgcccgcca gccccagttt caacgcggcc
     5101 gggaacccag gcacaaagac gggaatagaa cctaacaata cgccaagccc gatgccaata
     5161 aacactggca gcatctgaac ctgttgcagt ttttgctgcg cattccccag cacattggca
     5221 acggcatcaa ttgcggacgg acgccccacc agattgagga tatcgccgaa ctgcaggctg
     5281 atatcgccac tggcgaccag ttcgaccccg gcacggttca ggcgcgagat aacaacgtca
     5341 tagcgttctt taaagtgcag gtcgcgaata cgttttccga gcacgttttc attggtgacc
     5401 accacacgct cgacgcgcaa atcagtgcct ttcgtggaca gcgaagtatc gacctcctga
     5461 ccaatcacca gttgcgcatt atgtaaatcc gctggctgcc ccaccagatg cagcaaatcg
     5521 cccagttgga taatggtatc tggcgaagga acttttaggg tttcttcgcg tttcagacgc
     5581 gagcagataa ttttgtcgcc gttgagaatc ggtacatctt taatggctaa atcatgcagg
     5641 ttagggttct caacgcgaat attgatagtc ttgatcagcg caccgccatt ggtgcgtgaa
     5701 gactcgtgct gctgagcttc tgtctcgaca ttgacgcgga aaataacccg caacatccac
     5761 atggtgaaca aaatcccgca aatgccgaat ggatacgcca tcgcgtaact catccccatc
     5821 tgatcgacca tttccattgg tgtacccagg tcgcgcaaaa tctgctgccc tgcccccagc
     5881 gctggcgtat tggtaaccgc accggagaaa atccccagca ctaccggcag tggaatatca
     5941 aacagtttat gcaggatggc ggtaaccaga ccaccgatga tgacgatcag aacagcaaac
     6001 aggttgaggc gtaatccgga gacgcgcaat gaggcaaaga agcccggccc tacctgaatc
     6061 ccgatggtat aaacgaacag gatcaggcca aattcctgaa taacatgcag catatcgcta
     6121 cttaatgtca tccccgcctg agaaacaaaa tggccgacga tgatcccacc aaacagcacg
     6181 ccgccaatac ctaatcctat gccgcgaaat ttgacgttgc cgataaacaa accgacgact
     6241 gccaccaaag ccagaatact gaccgttaat gctatatcac tcatcgtcca tttctcttga
     6301 gataacattt taattgctac ggattatggc tgaatcggtg aagcagattt gccttatagc
     6361 gcacattatc acgggaaaga acgtgccgaa atatcttaaa cagtcgaaat ttaacgcatt
     6421 gttgagtaag taagacaaaa aaagccccac cagaatggcg gggcaaagag aatagctagt
     6481 tagctattta acgcgggacg ttcgctgata gcgatacgct gcgctgcgat gggttcaggc
     6541 tcattacgaa ttaaatcaat atgcagtaaa ccgtttacga aggttgcgcc agagacttcc
     6601 atattttcag ccagcgtaaa gctcaggcta aatggctgat tcataagccc ttgatgcagc
     6661 cattttttct cttcttttgg ctgctccggc gtgcctttta cgctcaggcg cgtaccttcc
     6721 agttgaatct ctaaatcttc ctgacggaaa cctgccagcg caagggtaat gcggtagtgg
     6781 ttatcgtcgc ttttctcaat gttgtacggc gggaagctct ggctttcacc ggcgttttgc
     6841 agtgcgttgg ccagtttgtc aaaaccgatc cattgacgca tcagtgggga taaatcgaag
     6901 ttacgcatag tcatttctcc ttctaagaag cgagtaagta cctgcaaatc cgaagattcg
     6961 catatgctcc ctgacggcga gcatggagat gtcaggccgc gccaggcggc cttaaggaat
     7021 tagttgattt cgatacggcg cggttttttc gcttccggaa tcacgcgttc gagatcgata
     7081 tacagcaaac catttaccag gttagcgcca cgaacatgaa tgttctcagc taactggaat
     7141 ttgcgttcaa agttgcgttc agcgatgccc tggtacagat aggtgcgctc tttttgttcg
     7201 tcggcgtgag cacctttcac caccagcaga ttatcctggg cggtaatttc cagttcgctc
     7261 tcagcaaaac cagccacagc gatagcaatg cggtaatggt tttcgtctac cagttcaacg
     7321 ttatacggag ggtagccgcc attactctgg ctctggttgt tttctaagtg gttaaacaaa
     7381 cggtcaaatc caatagcaga acggtaaagc ggggataaat caaagttacg cataatcaat
     7441 agctcctgaa atcagcgaga atgtaagacc ttccacaatg gacaggtcag gtagccagaa
     7501 cacccatcag gcgcgtcctc atcgactacg atgtaaaaat gggtctggaa atgaactttt
     7561 caagccttat cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc
     7621 agcacgaaac gttaaagtgc gatagccgcc acagagcgac gtaatgatga tgttattttc
     7681 agcaagggat cgctataact catcaggcca acacccacga tcaacagatg aatttattat
     7741 gataagaaat gtgttgttag cgttcatgat atgcagcgga atggcattac tcggaggatg
     7801 ctccagcgtg atgtcccata ccggagggaa agaaggaacg tatcccggca cccgcgccag
     7861 cgccaccatg attggcgatg atgaaaccaa ctggggcact aagtcgctgg cgattctcga
     7921 tatgccgttt accgctgtga tggacacgct tttattaccg tgggatgtgt tccgcaaaga
     7981 cagctcggtg cgctcacgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt
     8041 tattccaccg gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc
     8101 tggccacctt ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca
     8161 gaaggtggat tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca
     8221 caagcaattc gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat
     8281 gcagactgaa tatccgtttt gttatgggtt aactggcgcg gctcgccgcc ctgtggcgag
     8341 ataagccaca gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc
     8401 tgcggattac agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatgg
     8461 tgggtaaagg ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggcag ggtggtttcc
     8521 gttccactta acggcgcatc acctgccgct ttccagccag cttcatcttg cggcaactca
     8581 acgataaaca gctccggcac tttcacaccc tttggcgaaa gtgtgtcgcc aataaacgcc
     8641 agcgcgtgat ttcctaccca tccttcttca taagcgcgat tgatttcatc actgccaggc
     8701 tgcggcgtgg gcgtggtttt actcaccagc acacaccagt ggctgccgct gtattcacgc
     8761 ggatgctgct tttgtacatt gaccggacca aacggcgcag caacaccgac gtttcgcaaa
     8821 tccagcgccg ggctgcgttc atgcattaca tggtcgttat aggtaaagct caccctttca
     8881 ccgttcgggc taaagacatg cacatggctg ccgccgcgca gcgctcctgg cgtatacggc
     8941 gcggtaatat ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgc
     9001 cgatgatgga agtcgtaatg ccatgtttca tcaggatttt ccgggccgtg aataaagaca
     9061 tatttctctg acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcacag
     9121 tagataacct cgacctcacc ggtatggata ttcacacgct caatggtctc gccggtaaac
     9181 gacgccccag aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg
     9241 gtattggtga gcaggtgatt acggggagca aaggttatct gtttcatggc gcggtcctga
     9301 tacaaaacgg gtccagccat catacttcac aaggacttca ctttcagaca atagtctttt
     9361 gccatcgtga tgaatgtgag tgaaaagatg gaacatttcg acgtggcgat tattggcctc
     9421 ggcccggcag ggtcggcgtt ggcacgaaag ttagccggca aaatgcaggt gatcgcgctg
     9481 gataaaaagc accagcatgg tactgaaggt ttcagcaaac cttgtggcgg tctgctggca
     9541 ccggacgcgc agcgatcttt tattcgcgat gggctgacgc ttcctgtcga tgtgatcgct
     9601 aatccgcaga ttttcagcgt caaaaccgtc gacgtcgccg catcgctcac gcgtaactac
     9661 cagcgaagct atatcaatat taatcgccat gctttcgact tgtggatgaa atcgctgatc
     9721 cccgccagcg ttgaggttta tcacgacagc ctgtgtcgaa aaatctggcg tgaggatgat
     9781 aaatggcatg tcatttttcg tgcagacggc tgggagcagc atattaccgc ccgctatctg
     9841 gtcggtgccg atggtgccaa ctcgatggtg cggcgacatc tctacccgga tcatcaaatc
     9901 cgtaaatatg tcgcaatcca gcagtggttc gcagagaaac atccggtgcc gttctactcc
     9961 tgcatctttg ataatgcgat aactgactgt tattcatgga gtatcagcaa agacggttat
    10021 tttatctttg gcggtgccta tccaatgaaa gacggtcaga cgcgtttcac gactctgaaa
    10081 gagaaaatga gcgcctttca gttccagttt ggcaaagcgg tgaaaagcga aaaatgcacg
    10141 gtgctgtttc cctcacgctg gcaggatttt gtctgcggta aggacaacgc ctttctgatt
    10201 ggtgaagcgg cgggatttat cagcgccagc tcgctggagg ggattagcta tgcgctggat
    10261 agcgcagaca ttctgcgttc ggtgttactg aagcagccag agaagctcaa taccgcttac
    10321 tggcgcgcca cccgcaaact gcgtttaaaa ctcttcggca agatagtaaa aagccgatgc
    10381 ctgaccgcac cggctttaag aaagtggatt atgcgcagtg gtgtggcaca tattccacag
    10441 ttgaaggatt agccaacgcg cttcacatcg cccaccagca ggatataaga gagcgcgcca
    10501 atcaacgcga cggcggagat ataaaccagt gcaggtgcga aaccgtaacc ctgcgccagg
    10561 taccccacca ccagcggaac ggtgatgccg cccagaccac cggcgaagtt aaacacgccg
    10621 ccggttaaac caatcaggcg catcggtgcc agagaagaaa ccagcgacca ggtaatcgaa
    10681 gcaaaaccgt taccgaagaa tgccagcgcc atcaggcaca taatcatcat cggatcgtta
    10741 gtgtagttag cgcccataat gcaggtggag atcagcaagc cgcagataat cggcgtttta
    10801 cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat ccgctaccca gccagagagc
    10861 aggacgccga caaacgccgc gaggaatggc accgtggtca taaagcctgc tttcagcgcc
    10921 gtgattcctt tttcctgggt taaatagttc gggaaccagg ttaagaaaaa ccacagtgta
    10981 gaagccaccg caaattgccc aagataaacg ccgatcagtt tacgatggaa caccagtttc
    11041 cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca ccggcgcatc accatccacc
    11101 agaccgccgc catcacgaat gtaatccagt tcagctttgc tgataccttt ggtcaggcgc
    11161 ggcggctgat aaaccttaaa ccaaatcagc gaccagataa tgccgatacc accagtgaca
    11221 atgaacaccc agtgccagct caacatctcc tgaatccaga tcagcagcgg agtcaaaaac
    11281 gccagaccga caaactgacc agacgtataa aaaccaacgg cggaagcgcg ttcatgttcc
    11341 gggaaccagc tggtcaccat ccggttattg gtcgggaaag cgggcgcttc gaaaataccg
    11401 gttatcgcac gcagaccaat taatgacatt aatcccgtgg caaagccctg gaataacgtc
    11461 gccactgacc aaccaaatat cgcaataaaa taagtcacgc gagaacctac gcgatctaaa
    11521 aaccaaccgc cggggatctg gcatagcgta taaagccagg cgaaggccga aaatacatag
    11581 cccatttccg ctttcgtaat gccgaactct tcctgaatat gggcggaagc cacggccagg
    11641 ttagcgcggt caacataaca aatgaccacc gtaataaaga tcatcaccag cgtcagataa
    11701 cgccgacgcc ccggctttgc tgcattaacg ggaatatcca tagcgagctt tctccagatt
    11761 ttgggcatag cgaagccgct caccatgccc tgtaatttac agagggttat atttttgtat
    11821 tgctgtttta gtgcccgatg aggggcttac gtggcaggaa ttaccactct gctacgctgt
    11881 tatcttcatg acgccagagc ggattacgcc agtccggggc atttttactg aactcaataa
    11941 ctttagcttc gtcgatttcc acacctaagc ccggtttcgt taacggttta aagaagccgc
    12001 caaccatact gaagtcttct ttgtttttca caaagtcgag taactccgcg cctttgttgt
    12061 aatgaatgcc catactttgt tcctgaagta ccgcgttata ggaaacaaag tcgatatgca
    12121 ggcaagccgc cagtgcaatc ggtccgagcg gacagtgcgg cgcaagggtc acatcatagg
    12181 cttctgccat tccagcaatt ttgtagcatt cggtaatacc gcctgcatgg gagagatccg
    12241 gttgcagaat cgaaataccg cctgcctcca gcacgcgttt aaaatcgaaa cgtgagaaca
    12301 tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc caatttcggg tagtattcgg
    12361 cttgttccgc cagcaccggc tcttcaataa acagcgggcg atacggctcc agttctttaa
    12421 tcagcacttt cgccattggc gcgctgacgc gaccgtggaa atcaagacca aactcaatct
    12481 gattgccaaa agcttcacga atttgtgcca cggtgttgac tgccgcatct accgcgcggg
    12541 agttatcaat tagccccagt tcttcacaac cgttcagttt gaaggtatcg aagccgattt
    12601 cgcgcagggt tttaatgccg tcgataacat ccgccggacg atcgccgccg acccaactgt
    12661 aggctttaat tttgtcgcga accaggccgc ccatcagttg ccagaccggc gcattcagca
    12721 ctttgccttt gatatcccat aacgcctggt cgatcccggc aatggcgctc atcaggattg
    12781 gaccgccacg ataaaatccg gcgcgataca tcacttgcca taagtcattg atgcgcgaag
    12841 gatcctgacc aatcaaatag tcacccagct cgtgaactgc cgcttccacc gtacgggcgc
    12901 ggccttcaat cacgggctcg ccccaaccga ccacgccttc atcggtttca attttcagga
    12961 acatccagcg gggaggtaaa cgatacgtgg taattttggt gattttcatt gcactgcctc
    13021 tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg cgttctacgg actgcccggc
    13081 gcgatagaga tcgctgccta agcccgcccc agcacaacct gcgtctatcc actgcgccag
    13141 gttttctggc gtcacgccgc caacggaaaa gactgcgatg tccgatggca ataccgcttt
    13201 taacgctttg atgtattgcg gaccaaaagc cgatgacgga aatattttca gcgcctgcgc
    13261 gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg cagccggggc agacggtcat
    13321 gccgtagccc accgcacggc ggatcacttc actatggata ttgggcgtaa cgatgagctg
    13381 acagcccatc ctggcgagcg catcgacctg ttcaggtttc agtaccgtac ctgcgccaat
    13441 caacgccttg tcgccatacg catcaacgat ggcgggaatg ctttgctccc attgtgggga
    13501 attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc accgcgccaa catgcgccag
    13561 cgcctcgtcg ggcttaatgc cgcgcaaaat ggcgatcagc ggaagtttag tttgccactg
    13621 catgagcgat gctccttata ccagcctgaa atgccgtgtc gcccgccacc gccgtcacgt
    13681 cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt cagcgatgtt ccggcgacaa
    13741 gggtgatggc gtgttgatgg gtcacatagt cgcgcatact ggcaacctct gcgccaatca
    13801 acaaaccaga gagaaattcg ctgacctgtt cgcggggaag tgttcccagc acatgcgagg
    13861 cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt aaggccgcgc tcaaggccag
    13921 ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc tgcgccaatc aatgagtgat
    13981 ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg aaaatcgttg atttgctggc
    14041 tatcggcctg cacccatttg caatgcgtac cgggcataac ataaagagag gaaggagcca
    14101 gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat cacattgtgg ttatcgtcat
    14161 gagagacgca taacccggga ataatccaga tattgtcgcc aacagacgtt aattgttcgc
    14221 caatagacga aaaacgggca ggaacagata aatacggagc aactttccag ccgacattgc
    14281 tgccgaccat tcctgccatc accactggcg tattttcttc acgccagtcg gtcgtgactt
    14341 ctgctaacac cgcagccgga gattttccgt tcaggcgcgt gacgcctgct tctgattgcc
    14401 tgctctccag gcagtggtcg ccctgataaa gccaggcgcg cagattggtc gatccccagt
    14461 caattgcgat gtagcgagct gtcatgtgat ttcctttaac cttcgtgtcg agctggcgat
    14521 catggtaagc gccgcctgct ctgccgcatc gccgtcctga tgccgtattg catcgaacag
    14581 cgccttatgt tcctggagcg tttgcggcat gttggcctca tcgcccatcc aggttcgttc
    14641 aaaaaccgcc cgctgcaacg aactgatcgc aatgctaagt tgctgtaaca ccgggttatg
    14701 taccgactgc agcaccgcct cgtggtagcg aatatccgct tcgttaaacg cttcgcggtc
    14761 ctgattgttg gcaatcatct cgttcagcgc cgattcaatc tgcgccagat cgctggaagt
    14821 cgcgcgctct gctgcccaac gggcaatcgc cggttccacc agatttcgca cttcgctcat
    14881 ggcactgata agccgtgggt cgtagtcgtt ttccaacacc cattgcagta cgtcagtgtc
    14941 gaggtaattc cactggttac gcggtgccac aaacgccccg cgataacgtt tcatttcaat
    15001 cagccgcttc gccatcagcg aacggaacac ctcacggatg atgttgcgcg aggttgcaaa
    15061 ctcttcacag agctccgcct cagccggaag tggcgagcca ggtacgtatt tgccgtgaac
    15121 gatctgttta cccagcgtaa tgacaatgcg atcggtttta ttgagagtca tggagagtcc
    15181 ttgtgctctg tatgttcttc tctactttac cccgatcgat gcataacgcg gcaactttgt
    15241 agtaccagcg tgatgacgtt cgcgtttgcc gtgtgcgtaa tgtagtacaa acttatattg
    15301 ttgtactaca atttagatca caaaaagaac aatacataaa aaaatgacat gcgtcgggca
    15361 gaaatctgaa aagggatatc aggcgctaaa caggagggaa agaagagtat gctttcaacg
    15421 gcttagctac tcgtttaaag gattaatcat gaagttgaat tttaagggat tttttaaggc
    15481 tgccggttta ttcccactgg cgctgatgct ttcaggctgt atctcgtatg ctctggtttc
    15541 ccataccgca aagggtagtt caggaaagta tcaatcgcag tcagacacca tcactgggct
    15601 atcgcaggca aaagatagta atggaacaaa aggctatgtt tttgtagggg aatcgctgga
    15661 ttaccttatc actgatggtg ccgatgacat cgttaagatg ctcaatgatc cagcacttaa
    15721 ccggcacaat attcaggttg ccgatgacgc aagatttgtt ttaaatgcgg ggaaaaagaa
    15781 atttaccggc acaatatcgc tttactacta ctggaataac gaagaagaaa aggcactggc
    15841 aacgcattat ggttttgcct gtggtgttca acactgtacc aggtcactgg aaaacctaaa
    15901 aggcacaatc catgagaaaa ataaaaacat ggattactca aaggtgatgg cgttctacca
    15961 tccatttaaa gtgcgatttt atgaatacta ttcacccaga ggcattccgg atggtgtttc
    16021 cgcagcatta ctgccagtga ctgttacgct ggacatcatt actgcaccgc tgcaatttct
    16081 ggttgtatat gcagtaaacc aataatcagt aagcgggcaa acgcattcat gctgtttgcc
    16141 cgcccacaga ttaattcagc acatacttct caatagcaaa cgccacgcca tcttcaaggt
    16201 tagatttggt gacaaagttc gccacttctt tcaccgaagg aatagcgtta tccatcgcca
    16261 caccgacgcc tgcatattca atcattgcga tatcgttttc ctgatcgcca atcgccatga
    16321 tttcttccgg tttaatacct aacacgtcgg ccagtgattt cacccccgta cctttgttaa
    16381 cgcgtttatc gaggatttcg aggaagtacg gcgcactttt cagcacggta tatttctctt
    16441 tcacttcctg cggaatacgc gcgatagcct ggtcgaggat ggcgggttca tcaatcatca
    16501 tcactttcag gaactgggta ttggggtcca ttttctccgc ttcgcagaac accagcggaa
    16561 tggtggcaac gaaggattca tgcaccgtgt agtagctgat atcacggttg gcggtgtaca
    16621 gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc gcgagagagt ttttccagga
    16681 aacgatagtc gtcatagctg agagcagttt gcgccacggt gctaccatca gcggccttct
    16741 gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccatg tgcagctctt
    16801 tcaggtaatt gtgaacacct gcataagggc ggcccgtcgt cagcacgaca tttacgccac
    16861 gggcgcgagc tgcagcaatc gcatttttaa cggcaggtga aatggtgtga tcgggcagca
    16921 gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc ccaggtagat
    16981 tggttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac ccgagggaaa
    17041 agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt ttccctttca
    17101 gtagttccat gcctgcggaa agcagatcgt tattggcttg tggtgacact tcaccttgcg
    17161 gtgagagtgc atcaataatc ttcggcaatt gttctgccag taaactggaa gctgtactgg
    17221 tatccacgcc cagtttttgc ccgagatcgg acaccgcatt tgtgccgagc gctgattcca
    17281 gttgctcgcc gctaactggt tggttgccct gttgattact caaccaggtt gagagaatgg
    17341 cacctaaacc gccactttgc agtttttcca gcagtacctg aatgccgccc tgctcctcaa
    17401 cccagctcaa aatggcctga tatttccccg catcgccttt cagaaaggca ccgacaactt
    17461 catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgactca aaaagtatag
    17521 atctgcggga tgataattgc ggattgcaga aataaaaagg gcgaagatga tctccgccct
    17581 tttcttatag cttcttgccg gatgcggcgt taacgtctta cccggcctac aaaatcatga
    17641 aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc aggcacgctc
    17701 gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct cttcaataaa
    17761 cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaactggt cagcggcaat
    17821 cgcatcctta acggtaacgc gcagcatacg acgactttcc gggtccatgg tggtttccca
    17881 cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga gaccgcgacg
    17941 agactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct gacgacgctc
    18001 gccacgttcg ataaatgcat cttcttccag caagccacgc agtttctcac ccagcgtgca
    18061 gatacgacga tattcgccac cagtgataaa ttcgtgatcc agcggatagt cagtatccac
    18121 accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat tggtgtggac
    18181 atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt
    18241 cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg tcggctgata
    18301 gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga tcattttctg
    18361 cgtcgcgttg tactcagaca ccagtttctc taacgcttcg ccagccaatg ccggtgcgct
    18421 ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact gatccatcgc
    18481 ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca gcggcggctg
    18541 agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga agaaggtcaa
    18601 cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg
    18661 ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa gcgcggtgat
    18721 aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct caacgttgag
    18781 gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga
    18841 gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat cgcgttcctg
    18901 gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac ggcgggtcat
    18961 ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt tgccaaccac
    19021 gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt tcatctgctg
    19081 ttcaaccgct gatttcacct cagaagaaac cagtttgtct ttggtctggg aggagaattt
    19141 cgggtccggc actttcacgg aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt
    19201 ggcgctaact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt tcagggtacg
    19261 ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt
    19321 ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcaacgcca cttcgacgcc
    19381 aataccgtct ttttcagtgg agaagtagaa gatattcggg tggatcggcg ttttgttctt
    19441 gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt cttctttgcc
    19501 gtcgcgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca actcacgcag
    19561 acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt cgaggctggg
    19621 ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca acggtgcctg
    19681 cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga taaccagctc
    19741 cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac cgccggacac
    19801 tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga tcacttccgc
    19861 cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac
    19921 agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac ccgcgagcgc
    19981 ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc
    20041 cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt tcaggacttt
    20101 gatactggag gagtcataag aattcgacat caacgtttct cgctcattta tacttgggtt
    20161 aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc gacatgtcta
    20221 taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc
    20281 tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct atcaggtaga
    20341 gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt aaggcgcaca
    20401 tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa
    20461 tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa
    20521 aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc tggaaagaga
    20581 aagtcagaga aaactcaggg agaaattgct tacaagtatc ggccatatca gccgcgatac
    20641 cggcgctata ctccgcgcgc caggtgctga tttgctccgc cagcgggatc agttctttat
    20701 cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg cgctgcttga
    20761 gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga aagcatcccc
    20821 agtcgaggaa tgctcttctg tatttggggc cgccgttgag taaagtaaac ccttctggcg
    20881 ttatcaactg cattggcatc aggtgcgcca gttccgcgac cttatgcccg tctgtaccgt
    20941 cgatgcggac tttgctgtcg ccctgtttgt ctttggttaa gccaatcgct gtctcgcgct
    21001 cttcgccctg taatcgcccg tggagaacaa acgcctcctg ctcatggcga atgacgcgac
    21061 caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct tccagcacgc
    21121 tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat aaagcgagat
    21181 ccgcggtttc aatgttgcgg aaatcgcgga tcaacaagcg ggtgagggac attacagtct
    21241 cattggcatg acaacatagg cagccgactg tgatgcggca tcttcaatct gcacgctgga
    21301 aaccgaatcg gtcagcatca tgcggacgtt ttcacatttc agcgcgttca gaacatccag
    21361 cacgtagctg acgttgaagc cgatttccat ctccgcacca ctataggtaa cgtcgaggat
    21421 ctcttccgct tcttcctgtt ccgggttgtt ggcggtgatt ttcagctggt tttcgctgac
    21481 ataaagacgt acgccgcgga atttctcgtt agagagaatc gccgcgcgag caaacgcctg
    21541 cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggca gaacgcggcg
    21601 ataatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc caacgtgggc
    21661 gcgaatgttg ttgctgccaa tctgtacgcg cagcggattg tcgccgccgt cgagcatacg
    21721 catcagttca atcacgcctt tacgcggtac gatcaccgaa tggcttggca aagattgacc
    21781 aattggcatt gaacagaccg ccagacggtg gccgtcggtt gccacggtgc gcagttcttc
    21841 accttcggtt tcaaacagca taccatttaa gtaatagcga acgtcctgat gcgccataga
    21901 aaactgggtc gcttcaatca gacgcttcat cgttgcctgc ggcagggtaa attcgacttc
    21961 actctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg
    22021 gctacgcccg gagcgtacca gcatccgttc accttccagc tgcacggcaa tttccgcgcc
    22081 ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg
    22141 ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat cagtaccggt
    22201 cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg gtagcgtagg
    22261 acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct cacgttctac
    22321 ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa tcttctttga
    22381 tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc accgtcgtgt
    22441 ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc agctctttcg
    22501 ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga
    22561 gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg tcgatggtga
    22621 ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag tcgatggtga
    22681 tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct tccagctcac
    22741 gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc aaacgaatgt
    22801 cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct ggcggttcga
    22861 tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg ccgttgatct
    22921 ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc agggcgttga
    22981 aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga atatcgtcga
    23041 tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca
    23101 gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact ttggcattcg
    23161 gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta cccagacccg
    23221 tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc acctggcgag
    23281 ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac gtgtgtttga
    23341 cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc caacctgagc
    23401 gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt
    23461 tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca
    23521 gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta ttaaggtact
    23581 tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc gtgttatcgc
    23641 tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt aactcatcct
    23701 gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc actcgaacaa
    23761 aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg aaaagaccct
    23821 gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag atcctgcaaa
    23881 acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt tctcacagat
    23941 tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat cctgccgcga
    24001 ggcgggcacg atttacgccg catattggaa aatttaatga ccatagacaa aaattggctt
    24061 aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc cgtataatcc
    24121 tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt ccctgcgaaa
    24181 aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt acaattatta
    24241 caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt ttcggcggat
    24301 atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga aacgcacttt
    24361 tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta tggctactaa
    24421 aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc
    24481 taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag ttacgcttgt
    24541 taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc acgccgcaaa
    24601 ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt acagtcgcca
    24661 agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg cgtgaaagct
    24721 tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg
    24781 ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg cgccgccact
    24841 gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc tgattagtcc
    24901 gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa ttgaggcatt
    24961 gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat taaaatgcca
    25021 ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata ccagagaaca
    25081 ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt gtctttcatg
    25141 atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca gaccacgcag
    25201 acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag tggccagggg
    25261 aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg tggtggtgat
    25321 gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca gccgttccag
    25381 ctgctggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac cggtcgtgat
    25441 ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa agacgcttat
    25501 gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga cgcggcaggc
    25561 aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac
    25621 aacgtgcaga acgctggcga gaaaccgctg gaaatctcca cctttggtca gttgaagcaa
    25681 tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact gcacaccttc
    25741 cgcggcgcgg cgtactccac gcctgacgag aagtacgaga aatacaagtt cgataccatt
    25801 gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcaat gctgcaacag
    25861 tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta taccgctaat
    25921 ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt tcagcctggt
    25981 cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga caaaatggca
    26041 gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt catctctcag
    26101 ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg cttctccatt
    26161 atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc
    26221 tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga gcgtctgggc
    26281 gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga gaaggttaac
    26341 ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc gttgtactac
    26401 atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat ccacgacctg
    26461 tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat gttcttcatt
    26521 cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat gacctttatg
    26581 ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct gtactatatc
    26641 gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct ggaaaaacgt
    26701 ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct aaaataaggg
    26761 cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc atccgccaca
    26821 caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc cacgcctccg
    26881 ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga agttgccgaa
    26941 accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt taaagacgcc
    27001 gacggcagcg tgctcgatca ggggattgtg ctatggttcc ctggcccgaa ctcgttcacc
    27061 ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga cctgctgtta
    27121 aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc
    27181 gcgtttctta acgataaact tgacttagcc caggcagagg cgattgccga tcttatcgac
    27241 gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc attctccgca
    27301 cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga agcggcaatt
    27361 gatttccccg atgaagagat cgatttcctc tccgacggaa aaattgaagc ccagctcaat
    27421 gacgttattg ccgatcttga tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc
    27481 gaagggatga aagtggtgat tgccggacgt cctaacgccg gtaaatcgag cctgttaaac
    27541 gcgctggcgg ggcgtgaagc ggcaatcgta accgatatcg ccggaactac gcgtgacgtg
    27601 ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac cgccgggcta
    27661 cgtgaagcca gtgacgaagt agaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa
    27721 caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt ggatccggca
    27781 gagatctggc cggaatttat tgcccgtctg ccagcgaaac tgccgatcac cgtggtgcgc
    27841 aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta
    27901 attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca tctcaaacag
    27961 agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg ccacctacag
    28021 gcgctggaac aggcagcgga acatctacaa cagggcaaag cgcaactgtt gggagcctgg
    28081 gcaggtgaac tgctggcgga agagttgcgt ctggcacagc agaacttaag cgaaatcacc
    28141 ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg tattggtaag
    28201 taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc attgt
//
LOCUS       DAJSKJ010000029        28097 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000029 DAJSKJ010000000
VERSION     DAJSKJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.029"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..650
                     /locus_tag="ON345_001124"
     CDS             <1..650
                     /locus_tag="ON345_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3071219.1"
                     /translation="IWLITALGLVTTAGLAWVAKYLEITATKYDSPQMYVAIGLLLLC
                     MYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAPVPAIIVVFSTAGAMFLISMLAG
                     LLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVISCLMIVLWSGIISHGRNK
                     LLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(686..1642)
                     /locus_tag="ON345_001125"
     CDS             complement(686..1642)
                     /locus_tag="ON345_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR3071220.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1642..2883)
                     /gene="clsB"
                     /locus_tag="ON345_001126"
     CDS             complement(1642..2883)
                     /gene="clsB"
                     /locus_tag="ON345_001126"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR3071221.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFTAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2880..3641)
                     /locus_tag="ON345_001127"
     CDS             complement(2880..3641)
                     /locus_tag="ON345_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3071222.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLRETHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3774..4184
                     /locus_tag="ON345_001128"
     CDS             3774..4184
                     /locus_tag="ON345_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR3071223.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4146..5252)
                     /locus_tag="ON345_001129"
     CDS             complement(4146..5252)
                     /locus_tag="ON345_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3071224.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5263..6396)
                     /locus_tag="ON345_001130"
     CDS             complement(5263..6396)
                     /locus_tag="ON345_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3071225.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLSGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6389..8125)
                     /locus_tag="ON345_001131"
     CDS             complement(6389..8125)
                     /locus_tag="ON345_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3071226.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8118..9113)
                     /gene="hlyD"
                     /locus_tag="ON345_001132"
     CDS             complement(8118..9113)
                     /gene="hlyD"
                     /locus_tag="ON345_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR3071227.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9116..9787)
                     /gene="cecR"
                     /locus_tag="ON345_001133"
     CDS             complement(9116..9787)
                     /gene="cecR"
                     /locus_tag="ON345_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR3071228.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10016..11380
                     /gene="rhlE"
                     /locus_tag="ON345_001134"
     CDS             10016..11380
                     /gene="rhlE"
                     /locus_tag="ON345_001134"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR3071229.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11615..12094)
                     /locus_tag="ON345_001135"
                     /pseudo
     CDS             complement(11615..12094)
                     /locus_tag="ON345_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
     gene            12214..14364
                     /gene="dinG"
                     /locus_tag="ON345_001136"
     CDS             12214..14364
                     /gene="dinG"
                     /locus_tag="ON345_001136"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR3071230.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14392..15354
                     /gene="ybiB"
                     /locus_tag="ON345_001137"
     CDS             14392..15354
                     /gene="ybiB"
                     /locus_tag="ON345_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR3071231.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15495..16580
                     /gene="hcxB"
                     /locus_tag="ON345_001138"
     CDS             15495..16580
                     /gene="hcxB"
                     /locus_tag="ON345_001138"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR3071232.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNLA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16809..17069)
                     /locus_tag="ON345_001139"
     CDS             complement(16809..17069)
                     /locus_tag="ON345_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR3071233.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17334..17600)
                     /gene="ybiI"
                     /locus_tag="ON345_001140"
     CDS             complement(17334..17600)
                     /gene="ybiI"
                     /locus_tag="ON345_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR3071234.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17674..18351)
                     /gene="ybiX"
                     /locus_tag="ON345_001141"
     CDS             complement(17674..18351)
                     /gene="ybiX"
                     /locus_tag="ON345_001141"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR3071235.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFRQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18393..20675)
                     /locus_tag="ON345_001142"
     CDS             complement(18393..20675)
                     /locus_tag="ON345_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCR3071236.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20940..21200)
                     /locus_tag="ON345_001143"
     CDS             complement(20940..21200)
                     /locus_tag="ON345_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR3071237.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21476..22402
                     /gene="rlmF"
                     /locus_tag="ON345_001144"
     CDS             21476..22402
                     /gene="rlmF"
                     /locus_tag="ON345_001144"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR3071238.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22399..24624)
                     /gene="ybiO"
                     /locus_tag="ON345_001145"
     CDS             complement(22399..24624)
                     /gene="ybiO"
                     /locus_tag="ON345_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR3071239.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(24741..25463)
                     /gene="glnQ"
                     /locus_tag="ON345_001146"
     CDS             complement(24741..25463)
                     /gene="glnQ"
                     /locus_tag="ON345_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR3071240.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25460..26119)
                     /gene="glnP"
                     /locus_tag="ON345_001147"
     CDS             complement(25460..26119)
                     /gene="glnP"
                     /locus_tag="ON345_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR3071241.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26258..27004)
                     /gene="glnH"
                     /locus_tag="ON345_001148"
     CDS             complement(26258..27004)
                     /gene="glnH"
                     /locus_tag="ON345_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR3071242.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27408..27911)
                     /gene="dps"
                     /locus_tag="ON345_001149"
                     /gene_synonym="pexB"
     CDS             complement(27408..27911)
                     /gene="dps"
                     /locus_tag="ON345_001149"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR3071243.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTIAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         6652 a         7303 c         7475 g         6667 t
ORIGIN      
        1 gcatatggct cattaccgct ttaggtttgg tgacaaccgc aggactggcc tgggtagcga
       61 agtatctcga aattacggca acaaaatatg attcaccgca aatgtatgtc gccataggat
      121 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgtcatcg
      181 caggcgtaat atatctattt ttactctctt tggtggcgat tgtcgttgct agtttagctc
      241 ctgtacctgc cattatcgtc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
      301 tggccggttt attatttaat gttgatcctg gttctcaccg ttttatcatt atgatgacgt
      361 tgacagggtt ggccctggtg atcatcatga atgccgcatt aatgagtgaa cgacccgttt
      421 gggtaataag ttgcttaatg attgtgttat ggtcaggcat tatctcgcat ggacgaaata
      481 agctccttga attggcgggg aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
      541 ctgttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
      601 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag tcgtaaatgc
      661 agatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcttct
      721 tcgcctggct ttccagtagc aggtaacaga tcagcgccag cagcagcggg ataaagtaat
      781 acaggacgcg ataagcgagt agggcggcga taattgtgcc tttggatgta tgctccccag
      841 ccagtagcgc gataaacacc gcttccagca caccaattcc cgccggaata tgcacgatga
      901 caccagcaat actactaacc agcaacacgc ccagtacaaa gaaatagttc acgctttgac
      961 caagtaacag ccagattatc gctcccatta ccatccagtt aacgctggaa atcagcatct
     1021 gagcgagggc gaatttccat gaaggcagca ccagcttttg ccctttgatg gtcatatggc
     1081 ggtgcttcgc gaaagcgcaa aaccacaaat aaaccgcgat aatcatcagt aagccaatgc
     1141 cgagaatacg cagcgtagtt tgatcgacat accagtgatc cggcaactcc accacgcctg
     1201 cggtaaagat aatccctgcc agtaaaatat agcccagcca gttggtggta atactgagcg
     1261 agaaaatccg cgtaatagtg ctgcccggta accctagtcg ggagtacaaa cgatagcgca
     1321 taccaatgcc gccgacccag gtactgagcg tcaggttgaa ggcgtagcag ataaacgaca
     1381 ccagcatcac ctggcgcttc gccagtttgt gaccgcagta aaagcgggcg agcaggtcat
     1441 agcagccgta aatcagatag ctgacgacca ccagcccgac cgcactaagc agcgcaacgc
     1501 gattgtagtc gcggatgacc ttccagacct cttcccagtc cacttttttg gcgtagacca
     1561 ccagcaacac aatcaccgcg ataaaaaaca gccaggtgag gatcttcttt gctaagcgcc
     1621 agcgcgggtg tgatttactc atcagggttt tacccccgtg ttttcagttt ctacccgatc
     1681 ctgcgtttcc attgtcggtt gtgcgggcgg atcaacctgc gccagacgtg gcgtgtgtgc
     1741 cggaagccag ccaaccagcg ccgggaagtg gcgtaaaaag tggaacgcca gcacgctttt
     1801 ggtcaggttc caccaggtgc gtttgggcag catggtttca tccacctgct gacaatctgc
     1861 ggcgataatg ccgttcagat tatcgcgcag cgtctggtta aaattacgat cgtggatgat
     1921 gacatttgct tcgagattca gtgacaaact gagcggatcg agattactgg accctactgt
     1981 cgcccagtga tcgtccatca atgctacttt gccgtggagc gggcggcggc ggtactcaaa
     2041 cacctgaacg ccgcctttaa ccagatagtt atacagcaag cgcgcgccga ctctgacaat
     2101 cggcatatcc ggttcgccct gaatgatcag tttgatccgc accccgcggc gtgccgcttt
     2161 acgcaaggcg tgtaaaaatc gatagccggg gaagaagtag gcgttggcga taatcacttc
     2221 ccgccgcgcc tgagtgagca ttttcagata gtggcgttct atatcatcgc ggtgttcttc
     2281 gttatcgcgc cagaccagca atacctgcgc ttctcccggc tggcgatttt cttccgcttt
     2341 gtgatgacgt cgccaccagc gtcgggccgc gctttgtcct ggcagatttt ccagttcaaa
     2401 ctggaggata tcttcaacaa tcggcccttc aaggcgtacc gcgtaatcct gtttggcctc
     2461 tggaccgtag ctggacatat gctcggcgga gtaattcagc ccgccaataa aggctatacg
     2521 cgcgtcgatc accacaattt tgcgatgcat ccggcgaaac acattggtgc gcataccaaa
     2581 aaggcgaggg cggggatcgt agtagcgaaa taccacgcca gctgccgtca gttcattgac
     2641 aaactcatcg ctgagatctg gcgaaccgta gccatccagc aagacttccg ctttaacccc
     2701 gcgttgcgct gctgccagta gtgccgcatg cagctgtttg ccgacgtcat cctcaaacca
     2761 gataaacgtt tcaagaatga tgcgttcttg tgcctcgcca atcgccgtaa acaccgcggg
     2821 ataatattgc tcgccgtttt ccagcaactg gatcttattg ccttcgcgcc agctacattt
     2881 cataaatgaa tctccgcact taaaggggca tgatcagaaa ggtgtcgcca tgtccgcagc
     2941 ggcaacgcgg ttggcgcgct ggcgctggca tttttgacat agatcctgtc cagtcgtagt
     3001 agaggaaatt gcaccggaaa cgtgcgcgcc gggcgtccgt gggcgcgggt aaaaatctca
     3061 tccagtccgg cctgcacttt taacggatga ttagcttttt gccgccagtc attgaaatca
     3121 cccgccacca atactggttc gccgtccggc agctcattga cccattcggc gagcatcgca
     3181 agctgagcct gacggtgcgt ctcacgcagc cccagatgta cgcacatcac atgaatcgct
     3241 ttcccggtca tcggtggcac aatgcggcag tagagcacgc cgcgcttttc cgcaccatcg
     3301 accgaaacat cgcgattctc ataatgttca atgggatagc gcgacagtac ggcgttaccg
     3361 tgatgccctt ccgggtagac ggcattgcga ccgtaggcaa aatcgctcca catggtgtcg
     3421 gcgagaaact cgtagtgcga ggtatcgggc cagttttcca catgcagcgg atgaacttcg
     3481 tgcgcgccca tcacttcctg caggcaaaca atatcggcgc tgacggtacg cacggcgtcg
     3541 cgaagttccg gcaaaatgaa gcgtcggtta aacgcggtaa agcctttgtg aatattgatg
     3601 gtgagcacct tgaacgaaaa ttgttgtgtt tgatcgggca taattttcct gtctttgcct
     3661 cttatctcat tgaaatagtg tagtcggcgt cacaaaaagg tgcggtctta cggaattttc
     3721 cgtaaagttc ggtactctga gtaagtagag ataaattctt caggagagaa gccatgaagt
     3781 ggcaacaacg tgttcgtgtc gcaactggtc taagttgctg gcagattatg ttgcatttac
     3841 tggtagtggc gctgctggtg gtgggctgga tgagtaagac tctggttcac gtcggcgtgg
     3901 gattatgcgc actgtattgt gtcacggtag tgatgatgtt ggtgtttcag cgccaccccg
     3961 agcaacgctg gcgtgaggtg gcagacgtgc tggaagagct gaccacgacc tggtattttg
     4021 gcgcagcgct gattgtgctg tggctgttgt cccgtgtact ggaaaacaac tttttgctgg
     4081 caattgcagg gctggcgatc cttgctggcc cggcggtagt gtctttgctg gcgaaagata
     4141 agaagttaca tcaccttacg tctaaacatc gcgtacgccg ctgaccctgt cgtggccgtt
     4201 atcaccagta gcggccacaa actattccac acaatatcca gactcgcatc cttcaaataa
     4261 atctgcttgg taatgtccgt aaagtggcga atagggttaa tccacgtcag gttttgcagc
     4321 catactggca tgttttccac cggcgaaacg taaccggaaa ggagaatggc gggcatcata
     4381 aagacaaaca cgccgataaa cgcctgctgt tgtgtcgagc agagtgatga aatcaacaga
     4441 ccgaatccca ccagcgataa accgtaaatc accatcgtaa agtagaacag cgccagcgat
     4501 ccggcgaagg ggatttgata cgcccagata ccaatcgcca gcacaatggt ggcctggaag
     4561 gtggcgacaa ttaacgccgg tacggctttg ccgatgaaga tctgccaggt ggtgagcggc
     4621 gaaaccagta gctgatcgag cgtaccttgt tcacgttcgc gggcgacgga aagtgaagtg
     4681 acgatcatta cgccgatagt ggtgatcatg gcgatcagtg acggcaccac aaaccatttg
     4741 tagtcgagat tcgggttata ccagttgcgt accaccagct cgctgttgtt aggtttcggt
     4801 tttccttcca gcagctcctg ctgataattt ttgacgatct gttgcaggta gttggcggca
     4861 atttgcgcac tgttggagtt acgcccgtcg aggatcaact gcaacggcgc ggtctggaag
     4921 gtatccagtt tgcgcgagaa gtcagccggg aaacgcacca ggagtaacgc cttttgtgtg
     4981 tcgatggttg ggcggatctc ctgtgggctt ttcagcagca gcacatgagt aaaggcgctg
     5041 gcacgggcaa aacgttgggt cagctccacc gaatgctcgc cgttatcttc atcgtagatg
     5101 gcgatggtgg cgttagtcac ttccagcgtg gcggcgaacg ggaacagaat cacctgaata
     5161 agcacaggta aaatcagaat cgcgcgggtt tgcggttcgc gcagcagcga ctgcaactct
     5221 ttgcggatta acgtccataa gcgatgaaac atgctcttct ccctaatcca gccgacgttt
     5281 ggttttcagc cacgtcaggc cgataaacat caccgccgaa gcgatcaaaa acagcacgtt
     5341 taccaccagc accactggaa tattcccgga gaggaacagg ctttgcaggg tgctgacgaa
     5401 ataacgagcg ggaataatgt acgtcaccgc gcggatcacc gcgggcatac tgtcgatctg
     5461 aaaaataaag ccggaaagca taatcgacgg cagaaaagcg gcgttcaggg cgacctgagc
     5521 ggcattgaac tggttgcggg taatcgtgga aatcagcagc cccatcccca gggtactgag
     5581 taaaaacagg ctggagataa aaaacagaat cagcagcgac ccgcgatacg gcacgccgag
     5641 aataaacact gacaccagca tacacagcaa catcgccagc atcccgagaa agtaataagg
     5701 gatcagctta cacagcagca gttccgtgcg ggtaatctcc gtagagagca gagcctccat
     5761 ggtgccgcgt tcccattctc gcgccaccac cagcgaggtg agaatcgcgc cgatgaccgt
     5821 catgataatg gtcaccgcac cggggataat gaagtgctgg ctaatcgccg ccgggttaaa
     5881 ccagtagcgg gtttgtacat caataagcgg ttcaaaagtc tgcccgttgt cctccgctcg
     5941 ctgcatttgc cagatctgcc agatcccttc gacatacccc tgtacaaagt tagcggtatt
     6001 cggctcactg ccgtcggtga tcacctgaat cggtgcggtg gcgttggcgc gctccatctg
     6061 ttccgcaaaa tccaccggaa taaccaccag accgcgaatt ttccccgcct gcattttggc
     6121 gatcagttcc tgacggttat cgctgatggt ggcgtcgatg tagggcgaac cggtcatggt
     6181 gtgggtgaaa tccagcgccg cttcgctacg ctgttccagt aaaatcccga cccgcagctt
     6241 gctggagtcg aggttaatgc cgtaaccaaa aataaacagc agtagcagcg ggatcactac
     6301 cgcaatcagc cagctactcg gatcgcgaac gatctgccgc gtctctttaa cgcacagcgc
     6361 ccgtacgcga cgccaggaca ggatcgggtt actcattgct atgctcctta tcccagtcgt
     6421 ggatcaactg aataaaggct tgctccatgg tgggatcggg ttgctcgtcg ttggccgact
     6481 gcgctttcaa atcatccggc gtgccgctgg caattaattt cccacggtac accaggccga
     6541 tgcggtcgca atattctgct tcatccataa agtgagtggt gaccatcacc gtgacgcctt
     6601 tctctaccat gctgttgata tgcagccaaa attcgcggcg ggtgagggga tcaacgccgg
     6661 acgttggttc gtcgagaaac agaatgtccg gttcatgcat cagcgaacag gccagcgcca
     6721 gccgctgttt aaaacctaat ggcagttcat cggtcgcgtg ggaggcgata cttttcaggc
     6781 caaatgcctc gctcatacgg gagatttttt cgttctgcgc ccgaccgcgt aagccataca
     6841 caccagagaa aaagcgtaaa ttctgttcga ccgtcaggtt gccgtagagc gaaaattttt
     6901 gcgccatata gccgagatgc tggcgcgctt taccggaact ctctttcaga tccatcccca
     6961 gcaccagcgc ctggccggaa gtcggcacca gcaaaccgca catcatctta aaggtggtcg
     7021 atttacccgc gccgtttggc cccagcaaac caaaaatctc cccacgttta acggcaaagt
     7081 tgacgtgatc ggtggcggca aaatcaccaa atttcttggt cagttctttc gcttcgatca
     7141 ccgtctcgcc gggtgtgcct tctaccgtat gcaatattgc acccagcggc gattctgagg
     7201 ttccggcacc acccagcaaa tcaataaacg catcttcaaa acgcggcgta gtttcgttga
     7261 tgttgatttc cggcatcccg tcggcatggc gaatatcgtc tggtgtggcc tcttttttga
     7321 ggatcagacg taccgatttc ccctgaatca tgccgtcgct gacctgtggc agtttcaatg
     7381 cccgttgcaa cagtttgcgg ttgccctcgt gtggactggt catcagaaag ctgcgtccgg
     7441 ccatggtttg tgtcagggct tttggttccc cctgatacag caactcgcct tcattcatca
     7501 gtaacacgtc acggcactgc tcggcttcgt cgagatacga ggtactccag aggattaaca
     7561 tcccttcgcc tgccagctca tgcaccatct gccacagttc gcgccgtgag atagggtcaa
     7621 cgccgacgcc gggttcatcg agcagcaaca ctttcggttc gcccaccagg gtacaggcca
     7681 ggccgagttt ttgtttcatc ccaccggaga gcttgcccgc caggcgtccg gtaaacggcc
     7741 caagagacgt aaactccagc aggcgagcaa aagtttgctt acgtgcctcg ccggtgacgc
     7801 tgcgcaaatc cgcgtacaga ttgaggttct ccatcaccgt gagatcttca tacagaccaa
     7861 atttctgcgg catatagccg agcacggcgt gcagcgcgcc gtcgtttttg attggatcaa
     7921 agccaatcac cgtggcattg ccgctgtcgg gtttcagtaa tcccgccagc atccgcatta
     7981 gcgtggtttt acctgcacca tccggcccca ccagccccgt cacataaccg gcgtgaatgg
     8041 tacaatcgag cggcgcgacg gcgggcttgt ccatgcccgg aaagcgtttt tccaggccgt
     8101 tcagcgtgat aacggcatca ttcatgtcct gcctcgttac cgaattgcac cgtcaccggc
     8161 attccctggc gtaacgcatc atcggcgtcg gtcaccacaa tacgcaggcg atagacgagg
     8221 tcggtacgca gatccggtgt ttcgacggtt ttcggggtaa attcagcagt cggcgaaacg
     8281 aaaccaatct gcccgtgata cggcttgtcc gggcgaccat cggtataaag cagcactttg
     8341 cgccccggct gggcctggtc aagattccgt tcatcaacat aagcgcgcac ccacaccgga
     8401 cgcgttagtg aaacggtaaa caccgtgcca ccttcattga ggaccgtgcc tggctccacc
     8461 gcgcgcgtta acagcgtgcc atcagacggg gctatcaacg ttgagtcctg caaattcaac
     8521 tccgcctgcg ccagttgcgc ctgcgcctgt tcgaggctgg ctttcgcctg ggcgatgtcc
     8581 tgttcacggt taccggaacg gtactgacgc aatttatcct gtgctgattt cagcgttgcc
     8641 tgcgcctggt cgcgcgagga gcgggcattt tccaggtcgt ttgccgaaat ggtgcggctt
     8701 ttccacaacc cttgctggcg gttatagaag ttctgcgcat agtcataggc ggcttgcgcc
     8761 tgtttcaccg ctgcggcggc ctgagcgatt tcttcatcgc gatacccggc aagcatcagg
     8821 tcatactgcg cctgtgccac cgaaacaccc gctttcgcct gcatcagggc aatctcatac
     8881 ggcttgtgat ccagttcgcc cagcacctgg cccgctttga tagcatcacc ttcgtccacc
     8941 gccagcgatt caacgcgccc cccaacacgg aaactaagat tcaccgtacg aatatccacg
     9001 ttgccataca gcgtcaggcc gttatcctgg cggctttgat accaccagta gcctccggca
     9061 accacggcgg caagtaccac taccgccagt ccgatcacga caggtttttt catcactaca
     9121 aactcctttg cgataatcct tgcaaaatca ggtcgatatg acaggtcacc gtctggttga
     9181 tcagttcggt cttttcttca tcgaacgcgg tccagccggt acgtaacaga attgtttctt
     9241 tgccaagacg gaacgccaga atctcaccaa tcaacgcatg agtatggagg atcatgcggg
     9301 tatcgttggc gtcgcagccg gtccaggcgg caatcaggcg tgtcaggtgg ctgtgtagcg
     9361 ggctaatcac ctgttcgtgc accaggtgat aggctgccgt gggagagagc tgctcacggg
     9421 agataaactt gctgaggttg acggtgtcat cctgggtgag cagcttaatc atgttcctgc
     9481 aggcgcgaag gatcagttca cggatggctg cgcgatcagg ctgtggttgt gcgaacaagc
     9541 gttcggcctc ttcggcgtgc ggacggaact gctcgccaat aaaatcggca atccactggg
     9601 cgcaggcgag gtacaaatct tctttcgaac cgaagtagta ggtgatggca gcgatattct
     9661 gcccggcctg ggcggctatc tcgcgggtgg tggcgttcat tccatattca ccaaactgcg
     9721 ccagtgcggc agcaatcagc tgttttttcg cctgttcacc cttgattgtc atggcaggat
     9781 tattcatcgc acagtccatt cttaatcaaa tgattgatta agattatgac tccatatggg
     9841 agttgtccag tatggctaag aattttagct acgccagtca cagggataat ttatgcgctg
     9901 cgtcacaaaa actgctacac tccgctccct catgacattg tggtttttgt cattttcctt
     9961 ttcagtatct ccctgaaaac tacaccggta acggtcgggg cggttcggag tagttatgtc
    10021 tttcgattct ttgggtttaa gccctgatat cctgcgcgcc gttgccgagc agggttaccg
    10081 tgaacccacc cctattcagc agcaggcgat ccctgcggtg ctggaaggcc gcgacctgat
    10141 ggctagcgcc cagaccggta ccggtaaaac agcgggcttt acgctgccgc tgttgcaaca
    10201 cctgatcact cgccagccgc acgccaaagg gcgtcgtccg gtacgtgcgc tcattcttac
    10261 cccgacccgt gaactggctg cgcagattgg cgaaaacgtc cgtgattaca gcaaatattt
    10321 gaacattcgt tcgctggtgg tgtttggcgg tgtcagcatt aacccgcaaa tgatgaagct
    10381 gcgcggcggc gttgatgttc tggtggcaac accgggacgt ttgctggacc tggaacatca
    10441 aaatgcagtg aagctggatc aggttgaaat cctcgtcctc gatgaagctg accgcatgct
    10501 cgacatgggc tttatccacg atatccgtcg cgtgttaaca aaactgccag cgaaacgcca
    10561 gaacctgctg ttctctgcga ccttctctga cgatattaaa gccctggcgg aaaaactgtt
    10621 gcacaacccg ctggaaatcg aagtggcacg ccgcaatacc gcgtctgatc aggtgaccca
    10681 gcacgttcac tttgtcgata agaaacgcaa acgcgaattg ctgtcgcaca tgattggtaa
    10741 agggaactgg cagcaggtgc tggtgtttac ccgtaccaaa cacggcgcta accatctggc
    10801 tgagcagctc aataaagatg gcatccgtag tgcggcgatc cacgggaata aatcgcaagg
    10861 tgcgcgtacc cgtgcgctgg ctgattttaa atcgggtgac attcgcgtac tggtggcgac
    10921 cgatatcgct gcgcgcggtc tggatatcga agagttgccg cacgtcgtca actatgaact
    10981 gccaaacgta ccggaagatt atgtccaccg tatcgggcgt accggtcgtg cggctgctac
    11041 cggtgaagcg ttgtcgctgg tgtgtgttga tgaacacaaa ctgctgcgtg atatcgaaaa
    11101 actgctgaaa aaagagatcc cgcgcattgc gattccgggc tatgagccgg acccgtcaat
    11161 caaagccgaa ccgatccaga acggtcgcca gcaacgtggc ggcggcggtc gtgggcaagg
    11221 tggtggtcgc ggtcaacagc aaccacgccg tggggaaggt ggcgcaaaat ctgcaagcgc
    11281 gaaacctgca gaaaaaccgt ctcgccgcct cggcgatgcc aaaccggcag gcgaacaaca
    11341 acgtcgccgc cgtccgcgta aacctgctgc tgcgcagtaa tctttaatgc cgggctatgc
    11401 ccggcatcag gctgatgaac aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta
    11461 cgtgaactct gcaatatatt gaatttgcat acttttgtag gccggataag gcgtttacgc
    11521 cgcatccggc atttcacaac aagcacttgt cagcaatttg agaacacggg aaaaaattta
    11581 tctgttttac cgcccataag ccaccgagac gatacttctc tatagccaat tgttcgcgca
    11641 actccattaa aaggtagccc agtcgattct tgcccttacc attaccaccg tctccccagt
    11701 aagcatcgtt ttccgtatgc tcaaccagtt ttgcgggcgc ggttgccagc aagagcgcac
    11761 gcagttctgc atgctgttcg aatttagcgc gaagtgcttt tcgcatcacc tgttctttga
    11821 ccgactccca gtttttacgc agaggcttag aacgatcgcg ccccatgcgt gcggcgacca
    11881 taggggaaga aacccgacgg atctcttcgc ggtatttttc atcaaggaat ttttgtgcct
    11941 gaaaatagtg ttctgaggtg ggccaggttt tcccgtcaac cttgatgggc caggcggcaa
    12001 agttagaaaa atcaccgtag tcatcgctgg tgctgtagaa atttatgatg gtgtcttgca
    12061 tgacgtgttg gatcctttgt gctcgaacgg gcattaaacc gcattatgtt ggtggttatt
    12121 gcgagccgct ttccagaaac agaaaaacca ttacccctga aaaccgaaaa atgccacaat
    12181 attggctgtt tatacagtat ttcaggtttt ctcatggcat taaccgccgc gcttaaagcg
    12241 caaattgccg cctggtataa ggcgcttcag gaacagatcc ccgactttat tccccgtgcg
    12301 ccgcagcggc agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga agggcggcat
    12361 ctggcgattg aagcccccac cggcgttggg aaaacgctct cctatttgat ccccggcatc
    12421 gccattgccc gcgaagagca aaaaacgctg gtggtgagta ccgccaacgt ggcgttgcag
    12481 gatcagattt acagcaaaga tttaccgctg ctgaaaaaga tcattcccga tcttaaattc
    12541 actgccgctt ttgggcgtgg gcgctacgtt tgcccgcgta atctgacggc gctcgccagc
    12601 actgagccca cgcaacagga tctgctggcg tttcttgacg acgaactgac gccaaataat
    12661 caggaagagc aaaaacgttg tgcgaagctg aagggcgatc tcgacactta taaatgggat
    12721 ggtctgcgcg atcatactga tatcgctata gatgacgatc tctggcgtcg tttaagtacc
    12781 gacaaagcca gctgcctcaa ccgcaactgt tactactatc gtgaatgccc gttttttgtt
    12841 gctcgtcggg agattcagga agcggaagtg gtggtggcaa accatgcgct ggtgatggcg
    12901 gcgatggaaa gcgaagccgt actacctgac ccgaaaaatc tgctgctggt gctggacgaa
    12961 ggtcatcacc tgccagatgt ggcccgggat gcgctggaga tgagtgccga aatcaccgcg
    13021 ccctggtatc gactacagct ggacttgttc acgaaactgg tcgctacctg catggagcag
    13081 tttcgcccga agaccatccc accgctggcg atccctgaac gtttgaatgc gcattgtgaa
    13141 gagttgtatg agcttatcgc ctcgttaaac aacattctca atctctacat gcctgccggg
    13201 caggaggcag agcaccgttt tgcgatgggc gaactgcctg atgaagtgct ggagatctgc
    13261 cagcggctgg caaaactcac cgagatgctg cgtggtctgg cggagttatt tcttaacgat
    13321 ttaagtgaga aaaccggcag ccatgacatt gtacgcctgc atcggttgat tttgcagatg
    13381 aaccgcgcgt tggggatgtt cgaggcgcaa agcaaactct ggcggctggc gtctctggcg
    13441 caatcttccg gtgcgccggt gaccaaatgg gcgacgcggg aagagcgcga agggcagctg
    13501 catctctggt ttcactgcgt ggggatacgt gtcagcgatc agctggaaag gctgctgtgg
    13561 cgcagtattc cgcacattat tgttacctcc gcaaccttgc gttcgctgaa tagtttttcg
    13621 cgtttgcagg agatgagtgg tctgaaagag aaagcgggcg accgttttgt ggcgctggat
    13681 tcccccttta accactgcga acagggcaaa attgttattc cccggatgcg cgttgagcct
    13741 tccatcgaca acgaagagca gcatattgcc gaaatggcgg cctttttccg taagcaggtg
    13801 gagagcaaaa aacatctcgg tatgttggta ctgtttgcca gcggacgggc gatgcagcgc
    13861 tttctcgact atgtgacgga tttacgcctg atgctgctgg tgcagggcga tcagccgcgt
    13921 tatcgcttag ttgaactgca ccgcaaacgc gttgccaacg gtgagcgtag tgtgctggtg
    13981 ggcttacagt catttgccga agggcttgat ttgaaaggtg atctgctcag ccaggtgcat
    14041 atccacaaaa tcgcttttcc gcccatcgac agcccggtgg tgatcaccga aggggaatgg
    14101 ctgaaaagcc tcaaccgcta tccgtttgag gtgcaaagcc tgccgagcgc ctcgtttaac
    14161 ctgattcagc aggttgggcg actgattcga agccacggtt gctggggcga agtggttatc
    14221 tacgacaaac gcttgctgac caaaaattat ggcaagcgac tactggatgc attaccggta
    14281 tttccgatag agcaaccgga agtccctgaa ggtatagtta aaaagaaaga aaaaacgaaa
    14341 tccccacgcc gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta aatggactat
    14401 cgcaaaatca ttaaagagat cgggcgcggg aaaaaccacg cgcgcgattt agaccgggat
    14461 actgcgcgcg gtctgtatgc ccatatgctc aatggtgaag tccctgacct tgagttgggc
    14521 ggcgtattga ttgcgctacg tatcaaaggg gaaggggaag cagagatgct cggcttttac
    14581 gaagccatgc aaaatcacac catcaagctg acgccgcctg caggcaagcc gatgccgatt
    14641 gtcatcccca gttacaacgg cgcgcgtaaa caggccaacc tgacgccgtt gttggcgatt
    14701 ctcctgcaca aactcggttt tcctgtggtg gttcacgggg ttagcgaaga tccaactcgc
    14761 gtgctgacag aaaccatttt cgaactgatg ggtattacac caacgttgca cggcgggcag
    14821 gcgcaggcga agctcgacga acatcaaccg gtgtttatgc ctgtcggcgc gttttgcccg
    14881 ccgctggaaa aacaactggc gatgcgctgg cggatgggcg tgcgtaacag tgcacatacc
    14941 ctggcgaaac tggcgacgcc atttgccgaa ggtgaggcgc tgcgtctttc cagcgtttcg
    15001 catccggaat acatcggacg cgtcgcgaag ttctttagcg atatcggcgg gcgggcgcta
    15061 ctaatgcatg gtactgaagg cgaagtgtat gctaatccgc agcgctgccc gcagatcaat
    15121 ctcattgacc gtgaagggat gcgggtgctg tatgaaaaac aggaccctgc tggtagcgag
    15181 ttactgccac aagcaaaaga cccagaaacc acggcgcagt ggattgagcg ttgccttgct
    15241 ggcagcgaac cgattcccga atcgctgaaa atccagatgg cttgctgcct ggtggctacg
    15301 ggtgaagcgg caactatcag cgacggcctg gcgcgcgtta atcaggcatt ttaattcttt
    15361 tctcagccgg atgagccatg tcatccggct tttaccccac cgcaatatga aattcctgca
    15421 tctttattga ccttcccgcg cccggcgtgc agcataaaaa aacaacaaac acataacata
    15481 aacaggagtt aaccatggaa agtggtcatc gctttgatgc tcagacgctg cacagtttta
    15541 ttcaggctgt atttcgtcag atgggtagcg aggaacaaga agcgaaatta gtggccgatc
    15601 atttaatcgc ggcaaacctg gcagggcatg attctcatgg tattggcatg atcccaagct
    15661 atgtgcgttc ctggagtcag gggcatctgc aaattaacca tcatgccaaa atcgttaaag
    15721 aggcgggggc ggcagtcacg ctcgatggcg atcgcgcatt cggtcaggtc gcggcacacg
    15781 aagcgatggc gctggggatt gagaaagcgc atcagcacgg tattgctgcc gtggcgctac
    15841 ataactcgca tcatatcggc cgtatcggtt actgggcgga gcagtgtgca gcggcggggt
    15901 ttgtctctat ccactttgtt agcgtggtcg gtattccaat ggtcgcaccg ttccacggtc
    15961 gcgacagccg ctttggcacc aatccgttct gtgtggtttt ccctcgtaaa gataatttcc
    16021 cgctgttgct tgattacgcc accagcgcca ttgcatttgg caaaacccgc gtcgcctggc
    16081 ataaaggcgt ccccgtgccg ccaggttgcc tgattgacgt taacggcgtg ccgacgacca
    16141 atctggcggt aatgcaggag tcgccgttgg gttcgctgtt gacctttgcc gaacataaag
    16201 gctacgccct tgcagcgatg tgtgaaattc ttggcggggc gctttccggc ggtaaaacga
    16261 cgcatcagga aacgttacaa accagtcccg atgccattct taactgcatg accactatca
    16321 tcatcaaccc ggaactcttc ggcgcgccgg attgtaacgc gcagaccgaa gcctttgccg
    16381 agtgggtgaa agcctcgccg catgatgatg ataagccgat tttgctaccg ggcgagtggg
    16441 aagtgaacac gcgtcgcgaa cggcagaagc aggggattcc actggatgcg ggaagctggc
    16501 aggccatttg tgatgcagcg cggcagattg gtatgccgga agagacgttg caggctttct
    16561 gtcagcagtt agccagctaa aaaaaagccc gtccagtggc ggacgggcaa acaagggtaa
    16621 cataggatca atgagggtta gagcatatgc gtctgtcggc aaacagacag ggaaatactt
    16681 gtgctggacg tagcgtaaac gcctgatccg tccaacggtt cggattttgt aggcctgata
    16741 agacgcggta gcgtcgcatc aggcatctgt tgccggatgc ggcgtaaacg ccttatccgt
    16801 cctacgaatt acttataaat taccgcagta ccgcttaatt tattgttgtt ggtggcggaa
    16861 gtgatgctgt atccactggc accggctgcc gcggctttct cagccagttt cgcttccagg
    16921 gcatcgaggg tggatgcgcc atcggcagaa accacgccga ttttattcat gttctgtgcc
    16981 tgggatgccg ttaccggttc cgcggcaaac acgccaaatg acagagttga aagagccata
    17041 gcagcaacaa cagtattgat agttttcata attaatctct cgcaggtgat ttttgtataa
    17101 gtgacgttgt ttcgtcgatg tgatgagtat cacgtttttt tacgagagat aaaatcgaag
    17161 agaattgacc gccttgttca aataaattga ttgatatcta acatactgat aaaccattta
    17221 tttgttgtac ttatggactt gttttatgca gcgtggcagt cacgacagcg ttaaaggcac
    17281 ggaatgccac gcaaaataca aattatactc gctatacatc tcgccagttg tagttaacgt
    17341 aactggctgt ctttcgaacc tctgcgatta tatcctgtat aagctggttt ttgtaaatct
    17401 ttctcctgct gacaatgaat acataagcgc acgccaggaa tggcttcccg acgggcttgc
    17461 gggatggggg caccgcactc ttcacattca tacaggcttt cgccacgcgg aatttcaccc
    17521 cgagcgcggg caatcgcatc ttcaattgta ctgttgatct gttcgttgac ggcgtcatcg
    17581 ttagcccaac cggatgccat atcgacctcc ccatatcaat acttgtacag ttaagtgtag
    17641 ctaatccagg gacgaactcg ggcagttcaa gcatcagatc tccgaccatt cccgcagcag
    17701 attatgataa agattaagca gcgacaggat ctcttcactt tcgccgtagc ggcttttcag
    17761 cgactgaata ttgttgtcca gttcaaacag catggcgcgc tttttatcat cgcggatcat
    17821 cgactggatc cacataaatg atgccactcg tacgccgcgg gttacgggtg tcacgcaatg
    17881 caggctgctg gagggataca acacgagatc gcctgccggg agttttaccc gatgttgtct
    17941 gaaggtgtca ttaacgacca gttcgccgcc gtcgtagctt tgtggatcgc ttaaaaacag
    18001 cgtggcagaa aggtcagtac gcatccagcc gttttgtgga tggctgcgta ctgcgccatc
    18061 cacatggaaa ccataggttt cattgttctg atagcgatta aacagcggcg tggaaagggt
    18121 acgcggcaag gccgcggcaa agaacaaagc atgttggtta accgcgttca gcacctcatt
    18181 ttgcagggcg gcgtataacg tgctgcgggt gtcgacctgt tgattgttct taacttgcgc
    18241 accttgtgcg ccggtggtga cgcgtccatc cacccattcg gcttgttcca gttgttcgcg
    18301 aaaccgggcg acgtcctgtg gcgataacac gccgggaatg tggtacatca tacaagttct
    18361 ccaaaaagtg gggcctgcgc cccacatctg aatcagaaat gcatattggc tgtgagcaag
    18421 aaggttcttg gctcgcccgg gtgataacgg tagccgctct tgttgattga ggcgacgtaa
    18481 tcagtatcaa acaggttgta gacgtttagc tggaagtcga gattgcgatt aacccgatac
    18541 cccagtttgg catcggcgac ccagtaacct tcggtaaatg ctggcgttcc caccgcgccg
    18601 tctgaacctt tatgcatact gccgatatag cgtgcgcccg cgccaacaga gatatcgtcg
    18661 gttgcctgat attggctcca taaggtgaag gcgtgctccg gggtatacgg cagcgatgag
    18721 gaaccatcct gggcaacatc tttgccgttt ttgatggttg ctttttgctg ggtatagccg
    18781 ccaatcacct gccacgcggg agtgatattc ccggccacgg atatctcata gccttcgacg
    18841 cgtttcttac cgtattgcga gtaagttccg tcatcatttt gctcaacttc attttcgata
    18901 tcagtacgga acagcgcggc ggtgagcaac agacgtttat ccagaacctg ccatttggtg
    18961 ccaatctcgc tggtgttggc tttttgcggt ttaaaatcgg tgcggttggc actgttaccg
    19021 ctgccagact gcgcaagggc gaagttgttg ccgcccggag gctgctggga aacggcatag
    19081 ttaatataga cattaccgtt ttccgtcagg tgatacagcg ccccggcttt ccagttcacc
    19141 agattgcccg acttggcggt gtcgacggtg gtgaccggag aaccttttgc cacaccagct
    19201 gggcaggtga tggcaccgcg tccgctgccg ccgcaggcgg tggcactgtc atattcagta
    19261 tgataattat ccagacggat cccgccgttc agctcaaaat cacgggtgat ttgcagcgta
    19321 tcgaaggcgt aaattgcgaa ggtatccgtc tgaccattgg cgtttgcgcc gttgcgcgtc
    19381 aggccgccgg gatgaatgct gctgtcagga tgataaatat ttaccgcggg taacgtcacc
    19441 ggattaacgc cgtagttagt ctgcgtttca cgggtaaatt ccacgccggt actgacatca
    19501 tgaccgatag aaccggtata gaacgtcgag gtcaggttgg tctggttggt gagaatttta
    19561 ttactcacat ctttggtatt cgccgtgcgt gaccaggtcc agctattcac atcgctggtg
    19621 ggctgagtaa tattcgacgc cccgcccata atcgccgtca tcaggtaatc ctgctttacg
    19681 cgcgaccaac gggtagtatt gcgaatggtg gtgttatcgt tgatgtcgtg ctcaaaacgc
    19741 atggtggcgg tgtcggtggt cgaatcgtcg taatcggaat ccgtgccgta aaagttatga
    19801 gtatcaactt ttccggaatg attcagagcc gctgttcccg cagatggggc agaataaccc
    19861 ggcaaaccga tggtcggaat gccgccgtct ggcgtgttgt gctgggtgac atgcagataa
    19921 ttaagataca aacgattcgc tgtaccaagg ccaaaagcga tagaaggggc gacgccgtaa
    19981 cgctcatttt tgactttgtc gcgtccggca tcgtgcgttt tttcgcccat cacattcagg
    20041 cgcaccgcag tggtatcacc aatgacctga ttgacgtcca gcgtgccgcg gcggaaccag
    20101 gcgctgccaa tactggcgga ggcgtcaatg ccggaatcat tgcgcggctg cttgctgatc
    20161 atattgatcg agcccgtcgg cgcgctgcgc ccgtagtcgg tgccggaagg ccctttaatc
    20221 acttcgacct gctcagtatt gaaggtgtcg cgcgagacgc tgccgatatc gcgaatgcca
    20281 tcaatataaa tactgttaga ggtatcagca ccgcgcatat aaatggcgtc gccagtggtg
    20341 gagttaccgt tctcacccgc aaaaaacgca cccacgccgg ggacgttttt gagcgcgtcg
    20401 gtaaggttgg ttgcgccctg atctttaatc acttgttcag aaatcaccgt catcgtgcgg
    20461 gtagtatccg ctaccggacg cgagaatttc ggatcggcag attgttgtgg cgcataaagc
    20521 gaaggcgttg atgcttcgac aaccagcgtg tcatccgcgt tagtttgccc ttcggcggcg
    20581 agtgcctgag ccacaggcgt gatgccaata caaagaccgg caaagaacgt gagcgaatga
    20641 aattgtctgg cagggaaatt gcgattgttt tccattttgc aggtgacttt tttttatatg
    20701 ttgaaatttt cactgccacg agaaagcctc tctgcggatg cgatgccact gttaatctcg
    20761 tgatagtgtt catgcaagtg ataataattt tgataagcat tatcacttgt cgcgattttc
    20821 tatcacccat tgcgcgaaaa agaaatacat ttatttacat tgcgttcata tttgatgttt
    20881 tttgattaca aaacaggcga aaactgcatc cagaggcgaa tgcagttacg ttggtggggt
    20941 tatttgtaga tggttgcagt accgaacatc tggtcatttc cgcctgcgga attaatgaca
    21001 taacctttcg cgccttgctc gcgcgctttt tctgccagtt tatcctccag atcgcttagg
    21061 tttgatgcgc cagttgccga aacggtaccg gcgggccgta gctgaccgct gtccaggtta
    21121 ctcatcggtt gcgcggcata agccgtgaga gagattccgc tcaggacggt ggcaatcagt
    21181 agtgtgaggc actttttcat aatgacatcc tcataaatac aacgggtagt gcctgataag
    21241 tgtaggtcgc ctgggcgatg aaaatgacgc aaaaaattga cagagatgtg cttatttttt
    21301 gaacgatata tttttacaaa ataagacaaa tcaatgacat gcataatgct gatttttatg
    21361 atgaattgcc ataaaccata gctatgttaa ggttttgggg aatgataaac gcagccagat
    21421 atttaccctg tcgcgttatg cgggtatctt acgccgctgc ttaaaggaga atgctatgtc
    21481 cgcccagaaa ccggggttgc atccgcgcaa ccgtcatcac agccgctacg atctcgccac
    21541 gctttgtcag gtcaatcctg aactcaggca attcctcacg cttacacccg caggggagca
    21601 aagcgtagac tttgccaatc cgctggcggt gaaggcgctc aataaggcgt tgctggccca
    21661 tttttacgcc gtagcgaact gggatatccc cgacggtttt ctctgcccac cagtaccggg
    21721 ccgggcggat tatattcatc accttgccga tttactggct gaagcaagcg gaacaattcc
    21781 agcgaatgcc agcattctgg atatcggcgt tggtgcgaac tgtatttatc cgctgattgg
    21841 cgtacatgaa tatggctggc gttttaccgg tagtgaaacc agcagccagg cgttaagcag
    21901 tgcgcaggcg attatcagtg ctaatccggg gcttaaccgc gccattcgtc tgcgtcggca
    21961 aaaagagagt ggggcgattt ttaacggcat catccacaaa aacgagcaat acgacgcgac
    22021 cttgtgtaac ccgccattcc acgattccgc cgctgcggcg cgggcaggta gtgagcgtaa
    22081 acgccgtaac ctggggctga acaaagacga tgcactgaac tttggtggtc agcaacagga
    22141 gttgtggtgt gaaggcggtg aagtcaactt tatcaaaaag atgattgaag agagcaaagg
    22201 cttcgcgaag caggtgatgt ggtttacctc gctggtatct cgtggtgaaa acttaccgcc
    22261 gttgtatcgt gctctgaccg acgtgggcgc ggtgaaggtg gtaaaaaaag agatggccca
    22321 ggggcaaaag cagagtcgct ttattgcctg gacctttatg aacgacgagc agcgccgccg
    22381 ttttgttaat cgccaacgtt aaagcgttgg ctcccccggc ggcggcggtt cagccggggt
    22441 cgcgcccgga gcaggcagca cctgataagt ctgcactggc gcgcgaacgc ccgccaggtc
    22501 gaaatgtttt ttcacctggc tgtcgagggc aaagcgtacc gtccactgtt tgagtggcag
    22561 cgtggtgaac gaaacacgca gtgtaaacgc ggtattgctt aagccgacaa tcccggcaaa
    22621 attcggttca ccaataatca gcccgcgaat ttcttcgttt tccatcaatt ccgctaccgc
    22681 atctttcagt gcctgattgg ctttatcagc atcttcatgg cgatcaacat cgtagtttgc
    22741 cactaccgaa ccaataccgc ggacgaagtt agcaaaggtg gttatcgaag accacggaat
    22801 gatgtgatac gccccggtat cctggcgcac gcccacggag cgaatcgaca tccgttccac
    22861 tgtgccggtc aacggcccga tagtcaccaa atctccagtg ttcatgccgt tttcaaactg
    22921 aataaacacc ccggtaatga tatctttcac cagcgtttgc gaaccaaacg agatagccag
    22981 acctaatgcc ccggcacctg ccagcaatgg cgcgatattg acgccgattt ccgacaacac
    23041 aatcatgatg gtgatggtac tgataatcac cgccagcgcg ttacggaaca gcgtcagcag
    23101 ggtacgcgta cgggcgctgg gtagcgggcg gccatgaata tccgaagcca gccggttttc
    23161 gatcaaactg gcgagcaccg tccagccaac cgccgagaaa aaaagaataa gtgcgatacg
    23221 gatcaggata tctacggttt tctgccccgc gccgttttgc agccagttcc agaaatcgaa
    23281 caatccccat gcgctcaaca gcaacattac cgccacgcag acggtcagaa tacgcgccgt
    23341 tttcagcgcc gccgaaagcc agccattcag ccgtttttgc aactctggat agttacgctg
    23401 agtatgtggc gagagagtga tggtttttgc cagccagcgg gaaaacatac cggaaacaaa
    23461 cgctgcaata ccaataatcg ccaggctgcg caccgttgca cccatcataa atttcaggct
    23521 gttgcccgga tcgaacaacg aaaagaaaaa cagcacgata aaataggcgc ttgccagcca
    23581 gtgccacacc agcgcaaagg cgcggataaa caggctgaaa aaggccagcg aatgctccgc
    23641 aaagttgagc aaatgctggg taatctcttt tttattacga aagatcaggt acaacgccca
    23701 gacggtcatg cacagcataa tgatgacgtt cgccagcgca cctatctgta cattcacctg
    23761 attagagata atcggcacgg ccacaatcag gccataacct atcaaactgc ttaaccagct
    23821 caggcgacga ctccagtaac gggcgctctc gtcctgaatc gtgaacgggc gcagctccgc
    23881 cacgtttggg caaaaaatca ggcgtagtac ggctttgaaa aattcaatca gggcaaaggc
    23941 gttgagaaac aaactttgtt ggaaagcgat ggtacgactg cctgcattca ggttgtcgct
    24001 taatacctgg ccgacaaata atgtcagggc cagtaacagc aggtcgataa taaacgcccc
    24061 gataatcatc gccggaagct gcaaccagtt gctgcgctca cgattttttt gcctcgccca
    24121 ctgacccatt ttgcgataca gcggcagtgc gcacaggcga atcagccagt aaaaaccaaa
    24181 cactaatacc gctaacattg aaaaatggct cagcgcattg ctgaaggttt gtggattaaa
    24241 tggcttatgc ggggagccgg tgatattgcg ataaagttgc ccgaagcggg cggaaagggc
    24301 ttcaccataa tggcggctga cttcggtgac cttttgcagc acggtttgct cttcaaccag
    24361 cgtcggcggc acgatcttcg ggaccggttc cgcagggggc gtcgcggcga cagtacgcaa
    24421 ctggtcgatc aattctttac gcgaggtgtc gttatccagc acatccgcca gtgcgccata
    24481 ggccgctttt ttttgttcga tatccggttc cggggccggt tcagtcgttg agtccgttgt
    24541 cgttgtggtt gtaacgccgg gtatggatac cgcgtgggca ggtgcgccca gtaggcagaa
    24601 gaggatgaac aggatccacc gcatgactcc tccagtgaga aaacagagca aaaagataag
    24661 tatagatgct gggggagggg cgattttaaa tgagaggaat ctggtgtgcc tccctttcgg
    24721 gtgaaaggga ggcagagaag ttaagagacg tgctgcaaaa attcctgcaa gcgctggctc
    24781 ggcgggttct tgatcaatac ctgcggatcg ccatcttccg caatccggcc tttgtcgata
    24841 aagatcagcc gcgaagctac tttctcggca aaaccgattt cgtgggtcac gatcaccatc
    24901 gtcatccctt cttcagccag atcctgcata accttcagca cttcatggcg cagttccggg
    24961 tcaagagcgg aagtcggttc atcaaacagc atcattttcg gcttcaccgc cagcgcgcgg
    25021 gcaatcgcca cacgctgctg ttgaccacca gaaagttcgg aagggtagtg atgtgcacgt
    25081 tctgccagac cgactttcgc cagcagctca cgcgccagtt tttccgcctc ttctttgttc
    25141 gcgccacgca cgcgtagcgg accaaacatg acgttttcca gcgccgtcag atgcgggaag
    25201 aggtaaaact gctggaacac catacctgct tcctggcgaa tcaggcgctc gtcaactttc
    25261 ggatcgttaa ccttcaggcc atcgacaatc agatcgccgg aggtgatttc ttccagtttg
    25321 ttgatgcagc gcagcagggt cgatttaccg gaaccggacg gcccgataat caccacgact
    25381 tcgccctggg caatgttcaa atcgatattg tgcagcacct gggttgggcc aaagtgcttg
    25441 gagacgtttt taaattcaat cacaggattt tcatccttct ttccagacga cgcaaaataa
    25501 agctcagcac cagggtaata atcagataga acaccgctac ggcgctccag atctcaaggg
    25561 cacggaagtt accggcaata atttcttgcc cctgacgggt cagttccgcc acgccgatca
    25621 caataaacag cgaggtgtct ttaatgctga tgatccactg gttacccagc ggcggcagca
    25681 tacgacgcag tgccagcggt aaaatgacgt agcgaatggt ttcccaacgt gaaagaccga
    25741 gcgccagtcc tgcttcacga aaacctttgt gaatagacag caccgcacca cgcgtgattt
    25801 ccgcaatata cgcgccggag ttgatcatga tggtgaccac cgccgcagta aatgggtcga
    25861 tgcgtaagtc gttaaacgcc atcggcaggg cgaaataaat aaacatcacc tggacgacga
    25921 taggtgtgcc gcggatcact tcaataaaga ccagcgcgac gtggttggct atccaacctc
    25981 cgaaggtgcg tgcaaaacct gccagcaatc cgattaccag accgcctgcc agaccgagga
    26041 ccgaaatcca cagggtcatt ttggcacctt caatcagaag cggaatggca ggccagatgg
    26101 cactccagtc aaactgcata tgttgttcct gttaccgtgg tgtaaataat caaattcagg
    26161 gtaattaatg gccccggagc gggtttgcgc ccctcaccct aatcctctcc ccatagggga
    26221 gagggaactg ccagtgcgtt ttacaggtgt agcgttatta tttcggttca gtaccgaacc
    26281 attttttgta gatttcgttg taagttccgt tctcgcgcag ggttttcaac gcgccgttga
    26341 ctttgtcacg cagctcgtcg ctacctttcg ggaacgcaat accgtattgc tgcgcttcca
    26401 gagagtcacc taccgctttg aactgaccgt taccggcggt tttgatgaag tacagaatgt
    26461 ttggcgtatc gtgcagaacg gcgtctgcgc ggttggtgcc cagttccata taggcgttat
    26521 cgatgttcgg gaactgacgc agatctttag ttttgatgtt tgctttcgcg taatcaacgg
    26581 agccagtacc gctcttcaca gcaaccactt tcccgtcgag atctttcacg cttttcacat
    26641 cgttattgtt agctttcacc atcactaaca ggccgctttt gtagtagccg tcagagaaat
    26701 cgatcgcttt tttacgctcg tcggtgatgg taatgcccgc cagcgccaga tcgacgtttt
    26761 tggtttgcag tgccggaatg atcccactga aatccatcgg cttcagttcg taatccagct
    26821 tcagctcttt agcgatggca gcccacagat caacgtcaaa gcccacatat ttatcgccct
    26881 gtttaaattc aaacggaacg aaggcggtat ccgtcgcgac aactaatttt ttatccgcgg
    26941 catgagaaga aaccgcaaaa gccagggtca gtgcagccag tgaaactttt aatacagact
    27001 tcatagcatt tccttttttc tatccacggg acgatcccct gcgtgacatt cacatatatg
    27061 aaaaaatcgt gccagttttg caactccttg atttttcgag atgcggcaaa aacgtgatgc
    27121 acgatttata gggcaatacc ctgaagatgc accattctgg ggcaccaatc tggtgcgcta
    27181 aaattgtgca ctcaacacag tgattattta gcgtaaaacc tgatgaaaaa acaatcttta
    27241 tgtaacgatt gtgtgatgat gtggatacaa aaaatttact taatcagctg gagatagcag
    27301 atggatgcac taaataagtg cgttgaggtg ggctgcaaaa caaaacggcc tcctgtcagg
    27361 aagccgcttt tatcgggtac taaagttctg caccatcagc gatggattta ttcgatgtta
    27421 gactcgataa accacaggaa tttatccagg tcgcgagacg cggcggtcag gatatctgcg
    27481 gtgtcgtcat ctttcgcttc gccaatcgct ttgcgtacgt cattagcgac gattgcgtaa
    27541 cggtcagcca gttctttcag gtgatcctga acgttgtgga tgtccagcgg gtaacttttc
    27601 agcggggttt tgctgttgat aacttgagtg gtccccagag ctacaccgcc cagctgcact
    27661 gcacgttctg ctatggtatc cagatgatcg atcagtgcgg tgcgaaagcc atccagcatt
    27721 tcatgtacgg caatgaagtt agcgccgcgc atgttccagt gcgcttgttt ggtaatcaaa
    27781 gaaagatcaa taaactggat aacctggcga ttcagcaact ctactgttgc ttttttctcg
    27841 ctgtcggaga catcgttgcg ggtataaagc agattggtcg cttttgattt aactaattta
    27901 gcggtactca taatttcata tcctcttgat gttatgtccc agtaattaac gagattaagt
    27961 atagcaccgg ctatgtgttc cgctattctg gctgttccta tcacactaat agtggtaaca
    28021 agcgtgaaaa acaaaactaa taaagattca atgagttaga tatattgata agaacaattc
    28081 ttatttatct gctcgcc
//
LOCUS       DAJSKJ010000030        28006 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000030 DAJSKJ010000000
VERSION     DAJSKJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.030"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            84..635
                     /gene="maa"
                     /locus_tag="ON345_001150"
     CDS             84..635
                     /gene="maa"
                     /locus_tag="ON345_001150"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR3071244.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            751..1221
                     /locus_tag="ON345_001151"
     CDS             751..1221
                     /locus_tag="ON345_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR3071245.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            1385..2935
                     /gene="pdeB"
                     /locus_tag="ON345_001152"
     CDS             1385..2935
                     /gene="pdeB"
                     /locus_tag="ON345_001152"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR3071246.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2977..3330)
                     /locus_tag="ON345_001153"
     CDS             complement(2977..3330)
                     /locus_tag="ON345_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR3071247.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(3450..3546)
                     /gene="ffs"
                     /locus_tag="ON345_001154"
     ncRNA           complement(3450..3546)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ON345_001154"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3709..4020
                     /locus_tag="ON345_001155"
     CDS             3709..4020
                     /locus_tag="ON345_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR3071248.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(4051..4623)
                     /locus_tag="ON345_001156"
     CDS             complement(4051..4623)
                     /locus_tag="ON345_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3071249.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            4841..5701
                     /gene="tesB"
                     /locus_tag="ON345_001157"
     CDS             4841..5701
                     /gene="tesB"
                     /locus_tag="ON345_001157"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR3071250.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(5750..7036)
                     /gene="amtB"
                     /locus_tag="ON345_001158"
     CDS             complement(5750..7036)
                     /gene="amtB"
                     /locus_tag="ON345_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR3071251.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(7066..7404)
                     /gene="glnK"
                     /locus_tag="ON345_001159"
     CDS             complement(7066..7404)
                     /gene="glnK"
                     /locus_tag="ON345_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR3071252.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(7585..9366)
                     /locus_tag="ON345_001160"
     CDS             complement(7585..9366)
                     /locus_tag="ON345_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR3071253.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(9359..11131)
                     /locus_tag="ON345_001161"
     CDS             complement(9359..11131)
                     /locus_tag="ON345_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR3071254.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(11161..11619)
                     /gene="decR"
                     /locus_tag="ON345_001162"
     CDS             complement(11161..11619)
                     /gene="decR"
                     /locus_tag="ON345_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR3071255.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(11772..12590)
                     /gene="cof"
                     /locus_tag="ON345_001163"
     CDS             complement(11772..12590)
                     /gene="cof"
                     /locus_tag="ON345_001163"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR3071256.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMLLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            12690..14390
                     /locus_tag="ON345_001164"
     CDS             12690..14390
                     /locus_tag="ON345_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR3071257.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            14455..15150
                     /gene="queC"
                     /locus_tag="ON345_001165"
     CDS             14455..15150
                     /gene="queC"
                     /locus_tag="ON345_001165"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR3071258.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(15202..15600)
                     /gene="fadM"
                     /locus_tag="ON345_001166"
     CDS             complement(15202..15600)
                     /gene="fadM"
                     /locus_tag="ON345_001166"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR3071259.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(15694..16065)
                     /locus_tag="ON345_001167"
     CDS             complement(15694..16065)
                     /locus_tag="ON345_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR3071260.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(16216..18087)
                     /gene="ppiD"
                     /locus_tag="ON345_001168"
     CDS             complement(16216..18087)
                     /gene="ppiD"
                     /locus_tag="ON345_001168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3071261.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(18279..18551)
                     /gene="hupB"
                     /locus_tag="ON345_001169"
     CDS             complement(18279..18551)
                     /gene="hupB"
                     /locus_tag="ON345_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR3071262.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(18760..21114)
                     /gene="lon"
                     /locus_tag="ON345_001170"
     CDS             complement(18760..21114)
                     /gene="lon"
                     /locus_tag="ON345_001170"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR3071263.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQALPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(21302..22576)
                     /gene="clpX"
                     /locus_tag="ON345_001171"
     CDS             complement(21302..22576)
                     /gene="clpX"
                     /locus_tag="ON345_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR3071264.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDIQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(22702..23325)
                     /gene="clpP"
                     /locus_tag="ON345_001172"
     CDS             complement(22702..23325)
                     /gene="clpP"
                     /locus_tag="ON345_001172"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR3071265.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(23572..24870)
                     /gene="tig"
                     /locus_tag="ON345_001173"
     CDS             complement(23572..24870)
                     /gene="tig"
                     /locus_tag="ON345_001173"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR3071266.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(25214..25531)
                     /gene="bolA"
                     /locus_tag="ON345_001174"
     CDS             complement(25214..25531)
                     /gene="bolA"
                     /locus_tag="ON345_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR3071267.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            25836..26414
                     /locus_tag="ON345_001175"
     CDS             25836..26414
                     /locus_tag="ON345_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3071268.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            26458..27933
                     /gene="ampG"
                     /locus_tag="ON345_001176"
     CDS             26458..27933
                     /gene="ampG"
                     /locus_tag="ON345_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR3071269.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         6618 a         7623 c         6785 g         6980 t
ORIGIN      
        1 tcacacagat gtaatgggaa cgttctcttc actgactttt cgtcttactg tgttgccgca
       61 ttttcagcaa ccggaggtca gtaatgagca cagaaaaaga aaagatgatt gctggtgagt
      121 tgtatcgctc ggcagatgag acgttatctc gcgatcgcct gcgcgctcgt cagcttattc
      181 accgatacaa tcattccctg gcggaagagc acacattacg ccagcaaatt ctcgctgatt
      241 tattcggtca ggtgacagag gcttatattg agccaacgtt tcgctgtgat tatggctata
      301 acatttttct cggcaataat tttttcgcca acttcgattg cgtgatgctt gatgtctgcc
      361 ctattcgcat cggtgataac tgtatgttgg caccaggcgt tcatatctac acggcaacac
      421 atcccatcga ccctgtagca cgtaatagcg gtgctgaact ggggaaaccc gtcaccatcg
      481 gtaataacgt ctggattggc ggacgcgcgg tcattaaccc tggtgtgacc attggtgata
      541 acgtcgtggt agcttcaggt gcagttgtca caaaaggtgt cccggacaac gttgtcgtgg
      601 gtggtaatcc agccagaata attaaaaaat tgtaatcggt ttttcgcaac tgttatgcaa
      661 aattgtggta gatctgttac ttctcctcta ctattcccac gttaaaatag ggtgttccct
      721 ggaaagttgc agataccacg aaggcaaacg atgaccgaaa tacaacgcct gctgaccgaa
      781 acgattgagt ctctgaatac ccgcgaaaaa cgcgacaaca aaccccgctt tagtatcagt
      841 tttatccgta aacatccggg gctgtttatc ggtatgtacg ttgctttttt tgccaccctg
      901 gcggtgatgt tgcagtccga aacgctgtca ggctctgtct ggctactggt tgtattattt
      961 atcctgctta atggtttctt ctttttcgat gtctacccac gctaccgcta tgaagatatc
     1021 gacgtgctgg atttccgcgt ttgctataac ggcgaatggt acaacacgcg ctttgtacct
     1081 gccgcgctgg ttgaagccat cttgaactct ccgcgtgtcg cggatgttca taaggaacaa
     1141 ctgcaaaaaa tgatcgtccg taaaggtgaa ctgtcttttt acgatatttt taccctcgct
     1201 cgcgccgaat caacatctta agttagggtt acataccagg cgtaaagctc tgcgcctggt
     1261 caaatgacaa tgatcgtttc cacccatcac ttcatgaaat accagctcta cctccttatc
     1321 tcctgccagc ctttttccac aatcagatat actttcccta cactgtgtta ataaggatat
     1381 gctggtgaga acacgacatc tggtcggcct tatttcggga gtactgattc tttcagtatt
     1441 gctgcctgtc ggcttaagca tctggctggc ccatcagcag gtagaaacat cgtttattga
     1501 agagctggat acctattcct cccgcgtcgc tattcgggcc aataaggtgg cgacacaagg
     1561 gaaagatgcg ctgcaagagc tggaaagatg gcaaggcgct gcctgtagcg aagcccatct
     1621 catggaaatg cgtcgggtat cttacagtta tcgctatatt caggaagtgg tttatatcga
     1681 taacaacgtt ccccagtgtt cgtctctgga gcatgaaagt ccgcccgata ccttccccga
     1741 gccaggtaaa atttcgaaag atggttatcg tgtctggtta acatcgcata acgatttagg
     1801 cattatccgt tacatggtcg ccatgggaac ggcacattat gtcgtcatga tcgaccccgc
     1861 ttcctttatt gatgtcattc cctatagctc atggcaaatt gatgccgcca ttattggcaa
     1921 tgcccataac gttgtcataa ccagcagcga tgaaattgct cagggaatta ttaccaggct
     1981 acaaaaaaca cccggtgagc atatcgaaaa taatggaatc atttacgata tcctgccctt
     2041 cccggagatg aatatttcga tcatcacctg ggcttcaacg aaaatgttgc agaaaggctg
     2101 gcatcggcaa gtctttattt ggttaccgct cgggttggtg attggcctgc tggcagcgat
     2161 gtttgtgctg cgtattttgc gccgtatcca gtcaccgcat catcggctgc aggatgctat
     2221 cgaaaatcgt gatatttgcg tgcactatca gccgattgtc tccttagcca atggcaaaat
     2281 tgtcggtgct gaggcactgg cgcgctggcc gcagacagac ggtagttggt tgtcaccaga
     2341 tagttttatt ccgctggcac agcaaacggg cctttctgag ccattgacgc tactgattat
     2401 aagaagcgtc tttgaagata tgggcgactg gctgcgtcag catccacagc agcatatttc
     2461 gatcaatctt gaatccaccg tgctcacctc ggaaaaaatc ccgcaattgc tgcgtgaaat
     2521 gatcaatcac tatcaggtta atcccagaca gatcgcgctt gaactcactg aacgcgagtt
     2581 tgccgatccg aaaaccagcg ccccgataat ttctcgctac cgggaggcgg gccatgaaat
     2641 ttatcttgat gattttggta cgggatattc aagtttaagt tatttacagg atctggatgt
     2701 cgacattctg aagatcgata aatctttcgt tgatgcgctg gaatataaaa atgtcacgcc
     2761 gcatatcatc gaaatggcaa aaacactgaa actgaaaatg gtagcggagg gaatcgaaac
     2821 cagtaaacaa gaagagtggc tacgccagca tggcgtgcac tacggtcagg gctggctcta
     2881 cagcaaggca ttaccgaaag aagatttctt acgctgggcc gagcaacatt tgtgaatttt
     2941 tgggctgacg gcacgccgca cagcccgtca cgctacctat tcgtcgatga ttgactcgaa
     3001 tccgccatag atcattcgct tgccgtcaaa cggcatggac tcgccgaact ctttcatccg
     3061 tgggtccgac atcatctttt gattagcagc gtcgcggacc tcttttgaag ggtattcaat
     3121 ccagctaaag acaacctctt cattctcttc cgctttcacc gccatacgaa aatcggtcac
     3181 tttgccatcc ggtacatcgc tggcccagca ttcgacaata cgaagcgcgc caaactcttt
     3241 aaacaatggc gcagccttag cggccatttc ccgataggca tcctttttat cggcaggaac
     3301 ggcaaccaca aaaccatcaa catacttcat cagacatacc tccgaagcgt actgcgcagc
     3361 cagacaattc ttgcgacgtc agttgtggtt aaagtttagc catgttgggg ttttttgacg
     3421 aaacggtggg aggcagaaat gggtgggggc cctgccagct acatcccggc acacgcgtca
     3481 tctgccttgg ctgcttcctt ccggacctga cctggtaaac agagtagcgt tgcgggagaa
     3541 ccaacagagc ccccattgag agcgttgaga accaacgcgc aggcgcatta tcactgctgc
     3601 cccggctaat tgcaacccat cgcgtgccag atgctggttt cttgcgcaat gcgacttcac
     3661 tcgggcgttt ttccagatta tgctgaaaaa ttacctcagg aagaaaagat ggaaaaagaa
     3721 gattcatttc cccaacgcgt ctggcaaatc gtcgccgcta ttcccgaagg ctatgtcacc
     3781 acttacggtg atgtggcgaa actggcggga tcgccccgcg ccgcgcgcca ggtgggcggt
     3841 gtgttaaagc gtctccctga aggcagcacc ttaccctggc accgggtggt taatcgccac
     3901 ggcacaattt cgctaaccgg accggattta cagcgtcagc gacaggcatt actggcagaa
     3961 ggtgtgatgg tatcgggaag cgggcaaatc gacttgcagc gttatcgctg gaactactaa
     4021 ccctctccgg gcggagaggg ttaacgtcgc ttaatattgc gtaggagctg gaactgccga
     4081 agacggattc acctgagttg gtgaagtcga aggtacggta gtcgttgcac caccgctggc
     4141 ctgaaccggc acggcggttt gctgcaccgg caccaatgtc aggtcggctt tagttccgcc
     4201 cttgttgatc accggctgaa cggtatcggt aataaatacc agtttgtcat tcacggtaat
     4261 cgccgcactc aacagaatac gcgcgttcgg ctgaacatct gccgggttaa atggcagaac
     4321 aaagctgaat ggtgactgtt taccttcggt acgcaccgct ttctgcgcca gcacttttga
     4381 cggtgcatcg gctaacgacg cgtcagaaag tgtcacggtc agcacagcat caggcggcag
     4441 tgcgactttc tgccggatcc agacggtacc ggagacattc ggttgctgga tagcaggttg
     4501 ttgtgttgct gaaatagacg tatttgcagc cggggctggc gtctgaatat ccgcgctttt
     4561 atctgcgcaa gccgccaacg caatcgcaac cgctaaacca ctggccatgt gcacgagttt
     4621 cattcatttc tccttattat caatgcacca gcgggctaac tttcctcgcc ggaagagtgg
     4681 ttaacaaaat agtaacgtca ataagtgtgg cacacatcac gcatttctgc ctgtaattag
     4741 cccgtaattc agattattgc acccatcgga ccagtggcaa atttgcgtta tactcaactc
     4801 actttggctt gctgcggcag ctttgttact ggagagttat atgagtcagg cgctaaaaaa
     4861 tttactgaca ttgttaaatc tggaaaaaat tgaggaagga ctctttcgcg gccagagtga
     4921 agatttaggt ttacgccagg tgtttggcgg ccaggtcgtg ggtcaggcct tgtatgctgc
     4981 aaaagagacc gtcccggaag agcggctggt acattcgttt cacagctact ttcttcgccc
     5041 aggcgatagt aagaaaccga ttatttatga tgtcgaaacg ctgcgtgacg gtaacagctt
     5101 cagcgcccgc cgggttgctg ctattcaaaa cggcaaaccg attttttata tgacggcctc
     5161 tttccaggca ccagaagcgg gtttcgaaca tcaaaaaaca atgccgtccg cgccagcgcc
     5221 tgatggcctg ccttcggaaa cgcaaatcgc ccaatcgctg gcgcacctgc tgccgccagt
     5281 gcttaaagat aaattcatct gcgatcgtcc gctggaagtc cgtccggtgg agtttcataa
     5341 cccactgaaa ggtcacgtcg cagaaccaca tcgtcaggtg tggatccgcg caaatggtag
     5401 cgtgccggat gacctgcgcg ttcatcagta tctgctcggt tacgcttctg atcttaactt
     5461 cctgccggta gctctacagc cgcacggcat cggttttctc gaaccgggga ttcagattgc
     5521 caccattgac cattccatgt ggttccatcg cccgtttaat ttgaatgaat ggctgctgta
     5581 tagcgtggag agcacctcgg cgtccagcgc acgtggcttt gtgcgcggtg agttttatac
     5641 ccaggacggc gtactggttg cctcgaccgt tcaggaaggg gtgatgcgta atcacaatta
     5701 aaaaaatagc cggaggtgaa aaccgtccgg ctgttttttg cagtgcttgt tacgcgttat
     5761 aggcattctc gccgtgactg ttgacatcca gtccttctcg ctcctgctct tccggtacac
     5821 gcagaccaac cgtcagatcc gccagtttgt agccaataaa tgccaccaca ccggaccaga
     5881 cgatcgtaat ggcgatgctt tccagctgta ccagcaactg atggcccatc gtcacgcctt
     5941 cagcgaagcc cacgccaccc agcgaactgg cggcaaaaat cccagtcatg atacagccga
     6001 caatgccaca aacgccgtgc acaccgaaga catcgcaggg atcatccacc cgcagcaagc
     6061 gtttgagcat agtaacgccc cacaagcccg ccagaccagc taccacgccg ataatcaacg
     6121 cgccgccaac cccaatgtag ccacacgctg gcgtcacgcc gaccagaccg gcaatcgcgc
     6181 cagaacacgc tcccagcaga gaaggcttac cacgcagcgc ccattcaccg aagatccagc
     6241 caagaattgc cgccgccgtt gcgaccacag tattcacaaa tgccagtgcc gcgatttcat
     6301 tcgccgtgcc cgctgacccg gcgttaaagc caaaccaacc aatatagaga atggcagtac
     6361 cggtgaagac cattggcagg ttgtgcggtt taaacgcctc tttgccgaag cccacgcgtt
     6421 tgcctatcag atacgcgccc accagaccgg cgattgcggc gttaatatgc accaccgtgc
     6481 cacccgcgaa atccagcgca ccgtgagaag ccagcaaacc accgccccac accatatgcg
     6541 caatcggaat gtaagagagc gtcagccata ccaccacgaa aatcaacaca gctgagaagc
     6601 ggattcgttc cgccagcgcc ccaacgatca agccgacggt aatgcaggca aacgagccct
     6661 gaaacgccac gtggatatac tgataaatgc tgcccatcac cgccgtcagt tcgatgtttt
     6721 ttagcatcaa ccagttaatg ttgccgaaga agttgttgcc ctcaccaaac gccagcgagt
     6781 aaccgtaaac cacccagaga atacagacca gtgcaaacgt caccgtcacc tgcgtcagca
     6841 tcgacagcac gtttttgccg cgaatcaacc caccgtaaaa cagggcaatc cccggaatag
     6901 tcataaacag caccagcgca gtacaaatca tcataaacgc attgtcggct ttatcggcca
     6961 ccgcaggtgc agccattacc agtcccggca gcatcgccag tgaagcaagc ccagttttta
     7021 tcgtcgctat cttcattttt cgttcctgtt gctgtgtgcc agagattaca gcgccgcttc
     7081 gtcggcttcg ccggtacgaa tacgaatgac gcgttgcaat tcagcgacga agattttgcc
     7141 gtcgccaatt tttccggtgt aagccgcctt actgacgata tcgatcactt catcgagttg
     7201 gtcatcagca atcgccacat caatttttac ttttggcagg aagttgacgc tgtattccgc
     7261 cccccggtac agctcggcat gccctttctg acgcccgaaa cctttcactt cggtgacggt
     7321 caggccctga ataccaatgg aagataacgc ttcacgaacg tcttccagct tgaatggttt
     7381 gattatcacg gtcaccagct tcatagatcc cctccggtca gaattcggta atggttctgc
     7441 tacacgaaga aggtattgca agcggtgtgc cagaaatgaa aaacgagaaa gagagcagga
     7501 agttaagaag attgaaaatg aaaacgcact atgacagtgc acagtgcgtt acatttttgc
     7561 accaacgatg aaatattgcg ctattcaggc gctcaatgat tcttcttcac gcacgctggc
     7621 tgccagctct tcgcccgcaa gttgcagttg atacatctgc cagtagcgtc cctgggccgc
     7681 cagcagttgc tggtgagtgc cctgctccac ggcttgccca cgatgaagca ccagaatggt
     7741 gtcggcatca acaatggtcg ataagcggtg agcaatcacc accagcgtgg tatgttcacg
     7801 cacagccgcc agagcatgtt gaatcgcctg ttcagtaccg gagtcaatgc tggcggttgc
     7861 ctcatcaagg atcaggattt gcggcgtctc caccaacacg cgtgccagtg ccagcagttg
     7921 cttttgcccg acggagagat tattcccctg ctcgccaagc ggcgtataaa taccatcact
     7981 catgctgcgc gccagctccg ccagttgcac ggtttccagc gcctgccaga cgcgttcttc
     8041 ggagatatca cgccccagcg tcacgttggc gaggaaggta tccgccagca ccaccggatc
     8101 ttgctgcacc attgccacgc cctggcgcag cgcgctgtga cttagcgaac ttaatggacg
     8161 accatcaagg cgaatctcac cttccgttag cgggtaatag cccatcaata aactggcgag
     8221 ggtgcttttg ccactgccgg tatgcccgac cagtgccaca aagttacgcg aaggcacaga
     8281 gagattaatg ttctttagca ccagattgtc atcgcgataa gcaaatgaca cgttatcgac
     8341 ttcgatggtg ccactctgta acgggcgatc atcattgcca tattgctggc gcggtccgtc
     8401 catcagttca aacacgcgct caccagcaac aacagcctgt tgcagcatcg cctgttgcgt
     8461 ggtcagttcg attaatggtt cgttaagtcg cccaagatag ctgataaacg catacagcac
     8521 gcccacttca atggtgccgc tagcggagaa gccaaacagc atcaacaagc cacaaagaat
     8581 gagcgatgaa aacagactca gcagcggacg cagcagaaaa ccgtcgaggc gcagggtttg
     8641 catcctggcc atatagtgtg aacgactggc ctcccccata cgttcgccaa atcgcgcctg
     8701 ctgacggaac tgctggataa cgctcatgcc attgatgatt tcgttaaagc cgtcgttgat
     8761 atccgctaga taggcgcgca cacgacggac aatcggcgtg ctgtaacgct ggtatatcac
     8821 cattaccacc agcaccaccg ggaaaatcat tatcgccacc agcgccattc gccagtcgag
     8881 gctgaacatc gccaccagca tcgcgcccac cagtgcggcg ctacgcagga cagttgccac
     8941 tacggtaacg tagagatcgc ggatcacttc agtgtcatta gtgacgcggg aaatcacctg
     9001 cccgacgggt tgggtatcaa actcgcttaa tggctggcgc aatgcggcat ccatcacgtc
     9061 ggtacgcaac tgttgcacta cgcctactgc cgcccgatta aacagcagcg actgcgcgta
     9121 atgtagcccg gcggcaaaca gttgtagccc aacatacgcc gcagccagcc ctgcaaccat
     9181 tttcaacggc aggttatttt tcgctaccat attgtcgata aaatagctga taagcagcgg
     9241 cccactgact tctgccgctg ccgcaaccca catcatcagg accgcaatcc ccagcggttt
     9301 acgccacggc gaaccgtacg ctaacagacg cttgagagtc ggccacagtt ggctaaaact
     9361 acgcatcgac ggcctcctcg cgaatttccg gagcgtcgtc gagcgccgcc tccagttgtt
     9421 gatagcgata catatcgcga taccagccgc tttgttgcgc cagctcatca tgattgccac
     9481 gctgggcgat atgtccgtgc tgcatcacaa taatttcact ggcttccgtc agtgccgaaa
     9541 ggcgatgggc actgatgatt accgttcttc cctgtcccca ctgacgcagg ttatgcagga
     9601 tctggtgctc tgtgcgtccg tccaccgccg aaagcgcatc atcaaggatg aggatttccg
     9661 cgttgactaa taacgctcga gcaatggaga tacgctgttt ttgcccaccg gaaagcatca
     9721 cgccgcgctc gcccacttct gtatcgtaac cttgcggtag acgcaaaata tcgtcatgta
     9781 cgctggctaa ccgcgcgaca tgctcaatct cttgctgggt ggcattcggg caacccaacg
     9841 cgatgttgtt cgctacggtg tcggaaaaaa ggaacggtgt ctggctaact accgccaggc
     9901 ggctacgcca gctatcgagt tgtaacttcg tcagaggaat atcatgaaag cgaatatccc
     9961 cctcgctgac gtcgaaatga cgctgaatga gcgacaacag ggtacttttg ccggaaccag
    10021 tcggcccgca gatacccagc atctgaccgg gtttcagggc gaaattgacg ttttccagcg
    10081 caggatggtc agtctgcgga tacgtgaact ggtgaatatt tacatccagt tcgccacgcc
    10141 cttccggcac gggttcgcta ccatcgttca ccaccggcgc ttccgccagc atcgcgcgaa
    10201 tacggctgta tgcagcacta ccacgttcca caatgttaaa catccatgcc agcgccagca
    10261 ttggccaaat catcagacct aaatacatca taaaactggt gagctggccc agcgttaaac
    10321 tgccctgcgc caccatccaa ctaccgccgc caatcgccag caagttcgcc ataccaatcg
    10381 cgatatagat ggtcgggtcg aaacgagcat caatacgcgc cacccgcatg ttttttttgc
    10441 cggtatcttc ggcatccgcg gcaaataacg ccgactggcg atcttccaga ccaaaggctt
    10501 tgatcatgcg gatactggtg aggctttcct gggtgcggtc attaagacta gaaaacgccg
    10561 cctgtgccag cttaaagcgt tcatgcaagg catcgccgtt gcgcttgatc atgatcgcca
    10621 tcactggcat cggcaacagg gcaaataagg tcaactgcca gctaatttgc gtagacatca
    10681 taatcaacac agcgcagccc atcaccagtg aatccaccag cgtcagcacc ccttctccgg
    10741 cggcaaacac gacgcgatcg acgtcattgg tcgcacgagc catgagatca cccgtgcgat
    10801 gacgcaggta aaactcagga tgctgccggc ttagctgacg gtaataatct tcacgcagtt
    10861 caacagccag ttgataagac gcaccaaaca gcaatacccg ccagacgtaa cgcaggagat
    10921 aaaccacaac ggcaatcagc accatggtgg cgatccacat caggatctgc ccggtagtaa
    10981 agtgttgttc tgtcacgcca tcgacaacaa taccaaccac ttttggcgga accagttgca
    11041 gcatcgcgat aatgacaagc aaggcgacag ccccgagata gcgacgccat tcccgacgga
    11101 aataccagct taattgagca aataatcgca cgcgttgtgt cctgacctga ttctggatat
    11161 ttattcgatg ggtaaagaag tggtgtattt aatctgttcc atcgcgaagc tggaagtgac
    11221 gtccgacagc cccggcacgc tgtttaccag acgcttataa aactcgtcgt agcgtttcat
    11281 gtcggcaacc tggacgcgca tcagataatc gtattcacca gccatgcgcc agaaccccag
    11341 cacttctggc atttcggtaa ccaccgtgac aaagcggcaa taccattcgc tgctgtgatg
    11401 ttgcgttttt atcagcacaa aagcggtcag gccgaggcct attttttccg gatccagcag
    11461 ggcgactttg ccgataagga taccgtcgtc ctccagccgt ttcaggcgct tccagcaagg
    11521 ggtggttgtc agattaacgg cttcagccag tgcctgcaaa gagagggtgc aatcctgctg
    11581 cagtaaggcc agcagcttac ggtcaatttt atctaacata gcccttccac agagaatttt
    11641 tttctctttt aattctattt taaaggtgaa atcacgacaa tttattctgt gcggtttaaa
    11701 caccacggca caaaatgaca aattcaacgc aaaaaaaacc gggcaattgc ccggtgctgg
    11761 aagggatctc gttattcggg ggaataaggt agatgtggat agtccagcca gtgcgtcaaa
    11821 tagtgagaga cagcctgatt tcggcaatgt ccaatcaccg gtaaatgcgg gagctccgcg
    11881 cgcagttgcg gcatcgcatt gcccataata aacccgctac cgacgctgcc taacatttcg
    11941 cgatcgttca tcgcatcacc aaaggccatg caatcgcgca acgataaacc taaatgttgg
    12001 gtcagcaccg tcaatgcagc gcctttattg cagcccaccg gcagcacttc gaggcaatcc
    12061 gtggcggaaa aacacaaatg tgcacgctcg cctaatgctt cgtatagctg gatctgcaag
    12121 cgtgtaagat cgtcgtgatc gccacagaag cagatcttgg tgacgctgcc gagtagcatt
    12181 tttttgacat cgattatctg ataacgaaaa ccgctataga caaatgcctg caacaacgca
    12241 gggatctctt tcccggtaaa ccaaccgtcg tcattgaaga tatgcatgct ggctcgggta
    12301 tcccattgct gatacagcac cagctccgcg acatccgcag gtaaatcatc acgatgtaaa
    12361 agttcacctt ccagagaatg cacgcgcgtt ccgttgccgg taatcaaata tgcatccagc
    12421 gatagcgccc cgagaatatg ctgcatctcc agcgcatgac gccccgtggc aaaagtgagg
    12481 gtaatgtcgc gttcacgcag tcgcgccaaa gtagagaggg ttttctcacc taaatgatgg
    12541 tcaggcatca ataaagtgcc atccatatca aatgctgcca gacgagccat ttctttctcc
    12601 actctgtgac acggttaatg gtgattgagt atcacctgat atatacggaa gtaatagtga
    12661 atagttaaat aatattgttc cgggtttata tgcgattgct caaccgtctt aaccagtatc
    12721 aacgtctgtg gcaaccttca gccggaaagc cgcaaaccgt caccgtcagc gaactggccg
    12781 aacgctgttt ttgcagcgaa cgccatgttc gtacgctgtt gcgtcaggca caggaggcgg
    12841 gatggctgga gtggcaggcg cagtcaggac gcggaaagcg cggacaatta cgctttctgg
    12901 tcacgccgga atcgctacgc aatgcgatga tggaacaggc actggaaacc ggaaagcagc
    12961 aagatgtgct ggagctggcg caactggccc caggtgagct gcgcactctg ttacagccgt
    13021 ttatgggcgg acaatggcaa aacgatacac ccacgttgcg tattccctac tatcgcccgc
    13081 tcgaaccgct acaaccaggc tttttgcccg gccgtgccga gcagcatctc gccgggcaga
    13141 tattttccgg cctgacccgc ttcgataata atactcagcg cccgattggc gatttagcgc
    13201 atcactggga aacctctact gacgggttac gctgggactt ttatcttcgt tcaaccctac
    13261 actggcataa cggcgatgca gtaaaagcct cacacttaca ccagcgatta ttgatgctgt
    13321 tacaactgcc agcactggat caattattta ttagcgtgaa gcgtattgaa gtcacccatc
    13381 cgcagtgtct gaccttcttt ttacatcgcc ccgattactg gcttgcgcac cggctggcga
    13441 gctattgcag ccatctggcg catccgcaat tcccactgat cggcactggt ccttttcgct
    13501 taacacaatt cacagcggag ctggtgcgcc tggagagcca tgattattac catttgcgtc
    13561 atccgctgct taaagcggtt gagtactgga taactccgcc gcttttcgaa aaagatttgg
    13621 gaaccagttg tcggcatccc gtgcaaatca ccatcggcaa accggaggag ctgcaacggg
    13681 tcagccaggt cagtagtggc atcagtttag gtttttgcta tttaacgttg cgcaaaagtc
    13741 cccgactctc cctctggcag gcgcgaaaag tgatctccat tattcatcaa tccggtttat
    13801 tacaaacgtt agaagtcgga gaaaacctga tcaccgccag tcatgcatta ctgccaggct
    13861 ggactattcc gcactggcaa gtaccggatg aagtcaaact accgaaaacc ttgacgctgg
    13921 tttatcactt accgatagaa cttcatacca tggcagaacg cctacaggcg acactggcag
    13981 cggaaggctg tgaactcaca attatttttc ataacgcaaa aaactgggac gacacgaccc
    14041 tactggcaca cgcagacctc atgatgggcg acagattaat tggcgaagca ccggaatata
    14101 ctctggagca atggctacgt tgcgatccac tatggccaca tgttttcgac gctccagcat
    14161 atgcacatct gcaatcgacg ctggacgcgg tgcaagtaat gcctgatgag gaaaaccgat
    14221 ttaatgccct gaaagcggtt tttagccagt taatggcaga cgcgacgctg acgccgctgt
    14281 tcaactatca ctatcgcatt agtgcccctc ccggcgtgaa cggtgtgcga ctgacaccgc
    14341 gcggctggtt tgaatttacc gaagcctggc ttcccgcgcc gtcgcaatga atagctggtc
    14401 cgggttaatc gcaggcgtta ccataacgct tttattgttc aagcaggatt atctatgaaa
    14461 cgtgctgtcg ttgtgttcag tggaggccag gattccacca cctgtctggt gcaggcatta
    14521 caacaatatg atgaagtcca ttgcgtgacg ttcgattacg gtcagcgaca tcgcgcagaa
    14581 atcgacgtgg cacgcgaact ggcgctgaaa ctgggagcac gcgcgcataa ggttcttgat
    14641 gtaacgctgc tcaacgagct ggcggtcagc agcctgacac gtgacagcat tccggtgcct
    14701 gattatgaac ctgaagccga tggcatcccg aatacgtttg tcccagggcg taatattttg
    14761 ttcctgacgc tggcggcaat atatgcgtat caggtaaaag cagaagccgt aattaccggc
    14821 gtctgtgaaa cggatttctc cggctacccg gattgccgcg atgagtttgt gaaagcacta
    14881 aaccatgccg tcagtttggg catggcgaaa gatattcgtt ttgaaacgcc gctgatgtgg
    14941 attgataaag cggaaacctg ggcgctggca gattattacg gcaaactgga tttagtccgt
    15001 aacgaaacgt tgacctgcta taacggcatt aaaggcgacg gttgcggtca ttgtgcggca
    15061 tgtaatttac gcgccaacgg tttgaatcat tatctggccg ataaaccgac ggtgatggca
    15121 gcgatgaagc agaaaaccgg gttgaggtaa ttattccggg tgtcgccgga tgcggcttga
    15181 gcatccggca ccacaaaacg tttacttaac catctgctcc agcttttcgc gcaattcccc
    15241 ttccagagct aatgctttct gcgttttaag atcaatacaa acaaacgtaa taagcgcatc
    15301 cgctaccacc tgcccttccg gctccagtgt aatgacctgg cttaagatgc cgcttttacc
    15361 gtttaattgc tgcaactgac tggtaatagt taacaggtca cttaataccg ctgggcgacg
    15421 atagttaata ttgatattga ccacgacgaa ggcgatgtta tgggccgtca tccactgaaa
    15481 actgtcgcta ttttccaacc catcccagcg ggcttcttcg agaaattcaa ggtagcgggc
    15541 gttgttgacg tgctggtaaa cgtcgagatg atatccacga actttgattt gtgtttgcat
    15601 agcgcaataa ccttacgttg ttgttataag tacagaggtc ataactggta tgacctcttc
    15661 agtctggcaa aaaattgcca ctatgcaaat taattacagg gttaataccg ccagattacg
    15721 ttccaccagc gaattgccca tccccggcac ctgctttaga tcctccaccg ttttaaacgg
    15781 accgtactct tcgcgataac tgacaatcgc ctgcgctttc ttcaggccaa cgccattcat
    15841 cgcgcgggct agctcttccg cgctggcatt attaatgctg acccgggtgc cttcttcgtc
    15901 actggctttc gccggtactg ctgctttact ttgtgcagca ggagcttccg ctttggtttc
    15961 taccgccgtc ggtttcgcca cagaagctgc cgccagcgcg ctatgagaca ttccggcaca
    16021 ggccagggac agggtaatga gcagtgcttt aattccgtgt ttcatactgt tttctccttg
    16081 tttgttaaca gtggaatcac cttaacggca gtgaaaacag taaacaaatg gcaatgttca
    16141 gaaatggaaa aggccgcgaa agcgaccttt tcttgttaca gctgcaacgc atttttttgc
    16201 gaggcggctt caggattatt gctgttccag cgcatcgcca attttgattt tcgcctcttt
    16261 acgcaggtta ctcatcagag cttcaaagac gatttgtgcg ttgttctggg tgataccctg
    16321 caccatcgct tttttctgat cttccggcat tgaaccttgt ttcacttcat ccagcgccag
    16381 cagaaccaca ttaccttgca tatcggtcgc cataccgtag ctcggtttgt ctttcgctgg
    16441 cagtggcagt gcaaacgccg cctggctaat cgggtcacga ccggaacggc ttaaggtttt
    16501 cggctcgcca aatttcagac cggcagcctg catagcttcc gcacctttgc cggctttcaa
    16561 atcaaccagc agtttctcag catccacttt cgcctgttgt tcagctttgt tgtgctgaac
    16621 caatgcctta acttgttcct gaacatctgc caacggtttc accgcttccg gtttgtgctc
    16681 gctgatgcgc agcacgaatg cgcggtcgcc gtctacggtg atgatgtcag agttgatgcc
    16741 cggcgcgccg ttttcaccta ccagaccgcc gttaaagata gcgtcggcaa ccggcttgaa
    16801 gttcaactct tccggcaggt tatctttgct gaaccaaccc gtctgagtgg ctttaacgcc
    16861 ggcagcttgc tctgcaccgg ccagagactc ggtgtcgttg cttgccgcat cgctcacttt
    16921 ctgctgcagc gcatagtacg catcgagggc tttttcgtgt ttcactttcg ccgcaatgtc
    16981 gtcacgtact tcgtctaacg atttcacttt cgctggctga atgtcgtcca gacgtacaat
    17041 caggaaaccg accgaagatt tgatgacacc agacagttgc cctttttctt tcagaccagc
    17101 atttttcagt tcgtcgggga tagtggcatc ttctaaccaa cccatatcgc cgccgttacg
    17161 ggcagagata atatcggcag atttttcttt ggctaatgca gcaaaatcac cgcctttatt
    17221 cagctcatca agtaccgctt tcgcttcatc ttcagttttg gtctggatga tgctgtagcg
    17281 ggtacgctgc ggctgggtga attgatcctg atgctgatcg tagtagctct ggatatccgc
    17341 atcgctaacc ggttgctgca tcgttgcggc atccagcttg atgtaactca cgcggaattg
    17401 ttccggcgtc atgaaattgt ttttgttttg ttcgtagtag ctggcaatct cctgttcggt
    17461 cacaggctgc ttcgccgcca gcgcgttaac atcgatagtc gcctcacgca ccacgcgttg
    17521 ttgcgcgacc agtgccgcca gctcgtcggt ttcacctttc agcataaaat cggtaccggc
    17581 aacaccgtta atcagctgtt gggtagtgag ctggttacgc agcgcctggg cgtactgatc
    17641 ggcggtcatc cccatctggt tgaggatacc gttatagcgg ctgttatcaa atttgccgtc
    17701 aacctggaag gctggggtcg cgaaaatcgc ctgtttaacc tgctcatcac tgatacccag
    17761 tttcagctca cgagcgtact gatccaacag cgcctcgtcg atcagacgat tcagcacctg
    17821 ttgacgcagg gttttcatat agccttcatt cgctgccagc tcggagtatt gatcgcccag
    17881 ctgttgctgc atgcgattac gctcgctgtt aaaggcgttc tcgaactgcc cacggctgat
    17941 ttcctggtca ttcacttttg cggcgtagtt attgcctccg ccaatcaggt aaccactcac
    18001 gccggtcaat atgaacgaca cgataatgat accgaaaata atcttgagca cgagactgtt
    18061 tgcagccgtg cgtaagctgt ccatcatggt gtaacaacac tccgctgtag atgactgtgt
    18121 acctcacgca acgtatcgat gcgcgcaagg gcctattgtg acaagaaacg ggggcaattg
    18181 tcagcccaca agacgcagaa agtcaccgaa tacaaataaa aaaggcacat cagtagatgc
    18241 gcccttgaac ttcgtcacat ccccaccggg gacaacgctt agtttaccgc gtctttcagt
    18301 gctttacctg cacggaagct cggtacttta gcagcagcga tggtgatctc tttaccggtc
    18361 tgcgggttgc ggccagtacg ggcagcacgc tctttaacgg caaaagtacc aaaacctacc
    18421 agtgctacat catccccttc tttcagagat tcagttacgg aagcaataat agcatctaac
    18481 gcacggccag ccgcagcttt agagatatca gcccctgcag caatcttgtc gatcaattga
    18541 gatttattca ctcttctctt cctctttata atttatatcg cacttgaatc cttcaaggta
    18601 cgaacgcgca gcagttatat caggccagcc atccccttac aacacccctt aataagggca
    18661 agcccgatcc gccatctaac ttagcgagac aaaaaaaggc tggcaagccc gaattagcct
    18721 gccagccctg tttttattag tgcattttgc gcgaggtcac tattttgcag tcacaacctg
    18781 cataccagac ggttcatttt gtagcgccag agtcagaact tcctcaatgc gcttcacagg
    18841 atgaatgtcc agatcggcaa ttacgttgtc aggaatctct tccagatcgc gtttattttc
    18901 gaacggaatt agcactgttt taatcccgcc gcgatgcgct gccaggagtt tttctttcaa
    18961 accaccgatc ggcagtgcct gaccacgcag agtgatctca ccggtcattg ccacatcggc
    19021 acgaaccggg ttaccggtca ggcaagaaac cagcgcggtg cacatagcaa taccggcact
    19081 cggaccatct ttcggcgtcg caccttccgg tacgtggacg tggatgtcac gtttttcgta
    19141 aaaatcaggg ttgatcccca gtttttccgc acgcgcacga accaccgtta acgccgcctg
    19201 aatagactcc tgcatcactt cgccgagcga accggtatag gtcagtttgc ctttacccgg
    19261 aacacatgcg gtttcaatgg tcagcaagtc accgcccact tccgtccacg ccagaccggt
    19321 tacctgaccg acacggtttt cgttatcagc gcgaccatag tcgaaacgct gaacaccgag
    19381 gtagtcatgc aggttatcgc cgttaatttc gatatgtttt aatgacttat cgagcagtaa
    19441 ctgcttaacc gctttgcggc acagtttgga gatttcacgc tccagaccac gcacgcccgc
    19501 ctcacgggtg tagtaacgaa taatgccgat aatggcgcta tcgtcgacgg tcagctcacc
    19561 ttttttcagt gcattacgtt caatctgctt cggcagcagg tgacgtttgg cgatgttcag
    19621 tttttcatct tcggtataac cggagaggcg aatcacttcc atacgatcca gcagcggtgc
    19681 cggaatgttc atggagttcg acgtcgcgac aaacatcacg tcgctgagat cgtaatccac
    19741 ttccaggtag tggtcgctga acgctacgtt ctgctctgga tccagcactt caagcagtgc
    19801 agaggccgga tcgcctcgca tgtcagaaga cattttgtcg atctcatcga gcaggaacag
    19861 cgggtttttc acgcccactt tcgccatttt ctggatcagt ttacccggca tagaaccgat
    19921 gtaagtacgg cggtgaccac ggatttccgc ttcatcacgc acgccgccca gcgccatacg
    19981 gacatattta cgcccggtgg ctttggcaat ggactgacca agagaggttt tacctacccc
    20041 cggcggccct accaggcaga ggatcggtcc cttgattttg ttgacacggc tttgaaccgc
    20101 aaggtactca aggatgcgat ctttcacgcg ctccagacca taatggtcgg tatcaaggat
    20161 ttcctgcgcc tgacgcaggt cttttttgac cttgctgcgc gcattccacg gtacctgtac
    20221 catccagtcg atataaccac gcactacggt cgcttctgcc gacatcggag acatcatttt
    20281 cagcttctgc aactctgctt ccgctttctc ttttgcctct ttcggcatct tcgccgcgtc
    20341 gattttgcgc ttcagggctt cgttttcgtc cggcgcgtcg tccatttcac cgagttcttt
    20401 ctgaatagct ttcatttgct cgttcagata gtactcacgc tgggatttct ccatctgctt
    20461 tttaacgcgg ttgcgaatgc gtttctcaac ctgcagcaga tcgatttccg attccatcat
    20521 tgccatcaga tattccagac gttcgttaac gtcggacatc tccagaacgg actgtttgtc
    20581 agccagtttc agcggcatat gtgcagcaat ggtatccgcc agacgcgccg gatcgtcgat
    20641 gctattcagc gacgtcagca cttctggtgg gatttttttg ttcagcttga tgtagccttc
    20701 gaactggctg attgcagtac gcaccagcac ttcctgttcc cgctcatcaa tggtcggcga
    20761 ctccagatac tccgccttcg cagaaaagtg ttcgccattg tcagagagcg cagaaatacg
    20821 cgcgcgctgt aacccctcga ccagcacttt gacggtgccg tcaggcagtt tcagcatctg
    20881 caatatagag gccacggtcc cgacggtgaa aagatcgttt acacccggct catccgttga
    20941 agcttctttc tgcgcgacca gcataatttt tttatcatgg tccatcgcag cttccagaca
    21001 acggatagat ttttcccgcc cgacaaataa ggggatgacc atgtgcggat aaaccaccac
    21061 atcgcgcagc ggcaatacgg ggatttcaat gcgttcagaa cgctcaggat tcatagagct
    21121 ctctcttagt ttaatttccg ccaggtaatc agatgacacg actgtgcttc acgccatcta
    21181 ttaacatgta cgtcagtata tggggatgtt tcccccacat tcaacgccga gaatagagga
    21241 aaaattaaag gggagataaa atcccccctt tttggttaac taattgtatg ggaatggtta
    21301 attattcacc agatgcctgt tgcgcttccg gcttaccata aatcagcaac ggtttgcttt
    21361 gaccatcaat taccgactcg tcgataacca ctttttcgac gtcttccatg gacggcagat
    21421 cgtacatggt atcgagcagt gcggcttcta cgatggaacg caggccacgg gcaccggttt
    21481 tacgcgccat cgctttctta gcgatagcat ccagcgcctc gtcacggaat tccagatcca
    21541 cgccttccag attaaacagc gcctgatact gcttggtcag ggcgtttttc ggctctttga
    21601 ggatctgaat cagagcttct tcgctcagtt cattcaacgt tgcgacaacc ggcagacgac
    21661 caataaactc agggataaga ccaaacttga tcagatcttc cggttcaacc tgcgccagca
    21721 gctcgccttc gcttgctttg tcggacttcg cttttaccgt cgcgccaaaa ccaatgccgg
    21781 agccggtttc tacacggtgg gaaatcactt tatccagacc ggcaaacgca ccgccacaaa
    21841 taaacaggat cttagaggta tcaacctgca agaattcctg ctgcggatgt ttacgcccac
    21901 cttgcggtgg aacagcagct accgtacctt cgatcagttt caacagtgcc tgctgtacgc
    21961 cttcaccgga aacgtctcgg gtaatggacg ggttgtctga cttacgagaa atcttgtcga
    22021 tttcatcgat gtagacaata ccacgttgtg ctttctggat atcgtagtcg catttctgca
    22081 acagcttctg aatgatgttt tcaacgtctt cacccacata accggcttcg gtcagtgtag
    22141 tcgcgtcggc catggtgaac ggaacatcca gcaggcgcgc cagcgtttca gccagcagcg
    22201 ttttaccgga accggtcgga ccgatcagca gaatgttact tttgcccaac tcgacaccat
    22261 tgctggtatc gccgttgcgc agacgtttgt aatggttgta taccgcgacc gccagcactt
    22321 ttttcgcctg ttcctggccg ataacgtaat cgtccaggtg gttgcgaatt tcatgcggcg
    22381 tcggtagcgc actgcgttca cgatgcggtg caacttcttt aatctcttcg cgaatgatgt
    22441 cgttacataa atcaacacat tcgtcgcaga tatacacgga tggaccggca atcagcttgc
    22501 gcacttcatg ctggcttttg ccgcaaaaag agcaatacag caatttgcct gagccatctt
    22561 tgcgtttatc tgtcatgggt caaaacctct tctttgttct ttgtgccgca cacgacgacg
    22621 caaatgccat tctcaggcgc aagccgctta cagcgttgtg ccgccctgga taagtatagc
    22681 ggcacagttg cgcctctggc atcaattacg atgggtcaga atcgaatcga ccagaccgta
    22741 ttccaccgct tcaggggcgg aaaggaagcg atcgcgctcg gtatcacgtt caatctgttc
    22801 taatgattga cccgtatgaa gcgccataag ttcattcatg cgccctttaa ctttcagaat
    22861 ttcacgggca tgaatttcga tatcggtcgc ctggccctgg tagccgccca acggttggtg
    22921 aatcatcacg cgcgaattcg gcaggcaaaa acgtttacct tttgcccctg cggtcagcaa
    22981 gaaagcgccc atcgaggccg cctggcccat acagatggtg ctgacatcag gcttgataaa
    23041 ctgcatggtg tcatagatag acatcccggc agtgatcacc ccgcctgggg agttaatgta
    23101 cagatagata tctttttctg ggttttccgc ttccaggaac agcatctgcg ccacaatcag
    23161 gttagccatg tggtcttcaa cctggccagt cagaaaaatg acgcgttcct taagtagacg
    23221 agaatagata tcaaaagagc gctcaccgcg tgaggtctgt tcaatgacca tcggcaccag
    23281 cgccatatgg ggtgcaaagt tatctcgttc gccgctgtat gacatttccg tctcctggat
    23341 aaaattgaaa aaacctgctg taccgattgt aaccttatgg acggattatc agctagtccc
    23401 tgtgttatgg gtacggcatc accatatttc aagcataaca atcttttgtt gttacgctaa
    23461 caccgaaaga ggcttttcgc acggttccat gcaaaattca tgacaaaaaa aaagcccacc
    23521 acctggcggt gacgggcctt tgtgcgaatt tagcgcgtta tgctgcgtaa attacgcctg
    23581 ctggttcatc agctcgttga aagtggtttc tttttcagtc actttcgctt tcgccagtac
    23641 agcttcaaca gcctgttctt ccagagcaac attgcgcatg ttgtccatca gttctttgtt
    23701 tttgctgtag aactcgataa cttctttcgg atcttcgtac gcagaagcca tctcttcgat
    23761 caggcctttc acgcgctctt cgtcagcttt cagctcgttg gtgcggataa cttcgcccag
    23821 cagcaggcca acaactacgc ggcgtttagc ctgttcttcg aacagttcgc gcggcagttc
    23881 cagagcttgt ttttcgttgc caccgaaacg ctgtgcagcc tggcgacgca gaacgtcgat
    23941 ttcgctgtcg atcagcgcag ccggtacgtc gatgtcgtta gcttttacca gaccttcgat
    24001 cgcctgagac ttaacgcggt tacggatggc gctcttcagc tcgcgctcca tgtttttacg
    24061 cacttcagcg cgcagacctt ctacggaacc atcttcaacg ccgaaacgtt tgatgaattc
    24121 tgcagtcagt tccggcagtt cacgctcttc aactttcttc aggttgatag cgaatttcgc
    24181 tgctttacct ttcaggtttt ctgcgtggta ttcttccggg aaggtcacgt cgatggtgaa
    24241 ctcttcgcca gctttgtggc ctttgatacc gtcttcaaag cccgggatca tacgaccctg
    24301 gcccatcgcc agtacgaaat cagacgcttt accgccttcg aactcttcgc cgtctacaga
    24361 accggtgaag tcgatggtta cgcggtcttc tgcttcaaca gcgccgtctt tttctttcca
    24421 ggtcgcctgc tgtttacgca gagtatccag catgccgtca acgtcagcgt cggtcacttc
    24481 aacgatcggt ttttcaactt cgatcgcttc cagaccctgc agttcaactt ccggataaac
    24541 ttcaaactct acagagtaag tgaagtcttc acccagcttg tattcgcccg gaacataagt
    24601 cggtgcgcca gccggattga ttttttcttt aatgatggcg tcaatgaagt tacggctcat
    24661 caggtcaccc agaacgtcct ggcgtacaga cgcgccataa cgctgagcaa cgatattcat
    24721 tggcactttg cctttgcgga agccgtcaat acgtactttt ttcgcaacgt tgaccagctc
    24781 gcttttaaca gcggtctcga tgctgtcagc agcgatagta atcgttacac ggcggccaag
    24841 gccttgagtg gtttcaactg aaacttgcat cttgttacct caaaaaatca cagtgctcgg
    24901 tcaactctta ccccgcaagc acaggttgtt ggcttcgcgg aaccgggatg ttcttataat
    24961 caatcacatt ccctgttacc agaatcatcc cgaaagcatt caaataggac gcggcattat
    25021 agcggcatca cttttatgag tcgagaacgg ttattgcgcg ctgtcgcgta ttttttcaca
    25081 attcccgggc aattttcgtc tgaatgctgg gcaaaaagcg caaaacccag acaaaacaaa
    25141 acggcccgca ggccgttttt cataaaatct ttagcaacat actggaaaag cgccgacagt
    25201 tgcaaatgcg tttttacgcg atgcttcctg ctccacgaca gggaggagag gcaaagacgg
    25261 tgtcctgcaa cccttcccac tccttaatag tgtaagtatg cagagccagc gcatgaacgg
    25321 tagtagagag ttcctccgct aaagtactgt aaatcattcg atgacgattc agaaaacgtt
    25381 cacccgtaaa acgatcgctg accagcacaa ctttaaaatg gctttcagag ccggctggga
    25441 cattgtgacg atagctttca tccactactt cgaggaatac gggttggaac gccgccctta
    25501 atttttcttc tatccgctca cgtatcatca tgaaatatct cctccgacaa cgctgagatg
    25561 tcatccatcc ctttaaatac tagccgtttt taccgtttcc attgcagctt aacaacaaat
    25621 atttaggttt cgtcctggtt tttccgtcaa gcgtatgaag tttactgatt taaagattga
    25681 aacacaggcc tcgcatcagc gtttctttta ccaggggtga ttatttcgtc aacatgatga
    25741 atttacatgc cttacccact tcccctcgcc gttggcaatg ttatgatggc gggaattttt
    25801 accaaacacg cttaagagtc tctgagagcc cgaacatgtt caaaaaaatc ctcttcccgt
    25861 tagttgctct gtttatgctt gcaggatgcg caaaaccgcc aacaactatt gaagtttccc
    25921 cgacgattac actgccacag caggatccaa gcctgatggg cgtcaccgta agcattaatg
    25981 gtgccgatca gcgtaccgat caggcgctgg caaaagtcac ccgcgataat caaatcgtta
    26041 ccctgaccgc ctcccgcgat ctgcgtttcc tgctgcaaga agtgctggaa aaacagatga
    26101 ccgcgcgtgg ttacatggtt ggtccgaatg gcccggttaa tctgcaaatc atcgttagcc
    26161 aactgtatgc tgacgtgtcc cagggcaacg tgcgctataa catcgctacc aaagcggata
    26221 tcgccatcat cgctaccgcg cagaatggca acaaaatgac caaaaactat cgtgccagct
    26281 acaacgttga aggtgcgttc caggcctcca acaaaaatat cgccgatgcg gttaacagtg
    26341 tgctgaccga taccatcgct gatatgtctc aggacaccag catccacgaa ttcatcaagc
    26401 agaacgcgcg ttaatttctg ccctctggcc cggtgcaagc cgggcctgta gacgcccatg
    26461 tccagtcaat atttacgtat ttttcaacag ccgcgttcag ccatattgct gatcctgggt
    26521 tttgcttccg ggctaccgct cgccctgaca tccggtacct tacaggcctg gatgacggtc
    26581 gagaatattg atctcaaaac cattggtttc ttctctctgg taggccaggc ttacgttttt
    26641 aaattcctct ggtcaccgct gatggaccgc tacacgcccc cattttttgg gcggcggcgc
    26701 ggttggctgc tcgccacgca aatcctgtta ttagtcgcca ttgcggcgat gggttttctc
    26761 gaacctggca cccaactccg ctggatggcg gcactggcgg tggtgatcgc tttttgctcg
    26821 gcctcccagg atattgtctt cgatgcgtgg aaaaccgatg tgctcccggc agaagaacgt
    26881 ggtgcgggcg cggcaatcag cgtgctgggt taccgtttag ggatgctggt ttccggcggc
    26941 ttagccctgt ggctggcaga taaatggctg ggctggcagg gcatgtactg gttgatggcg
    27001 gcgctgttga tcccttgtat tatcgcgacg ttgcttgcac cagaaccgac cgacaccatt
    27061 cctgtgccaa aaacgctgga acaagcggtt gttgcacctc tgcgagattt ctttggtcgc
    27121 aataatgcct ggcttatttt gcttcttatc gtgctgtata agctgggcga cgcattcgcc
    27181 atgagcctga caaccacgtt tttgattcgc ggcgtcgggt ttgatgcggg tgaagtaggc
    27241 gtggttaaca aaacgcttgg cttactggcg accattgtgg gcgcattgta cggtgggatt
    27301 ttgatgcagc gcctgtcgct gttccgggcg ctgctgattt tcggcatttt acaaggtgcg
    27361 tctaacgctg gttactggct gctgtcgatt actgataagc atctctacag catgggcgca
    27421 gccgtctttt tcgaaaacct ctgtggcggg atgggcacat cagcctttgt cgcgctgtta
    27481 atgacgctgt gtaataagtc attttccgca actcaatttg ccctgctctc agcgctttcc
    27541 gctgtagggc gagtttatgt cggccccgtg gcgggttggt ttgttgaagc acacggctgg
    27601 tcgacattct atctattctc cgtcgccgct gccgtaccgg ggcttatttt gctgctggtt
    27661 tgccgccaga cgcttgaata tacacgagta aatgacaact ttatctcccg taccgaatat
    27721 ccggcaggtt atgcctttgc catgtggaca ctggcggcgg gcgtcagcct gttggccgtg
    27781 tggttactgc tgttgacgat ggacgcgctg gatttgacgc acttctcttt cctgcctgct
    27841 ctgctggaag tcggggtttt agtcgccctt tctggcgtcg tgcttggtgg tttgctggat
    27901 tatctggcgc tacgaaaaac gcatctgacg taatctgtaa atattattta gctgtagtaa
    27961 tcactcgccg taattattac ggctaaataa acatcagtat cgctta
//
LOCUS       DAJSKJ010000031        27881 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000031 DAJSKJ010000000
VERSION     DAJSKJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.031"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..263
                     /locus_tag="ON345_001177"
     CDS             <1..263
                     /locus_tag="ON345_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3071270.1"
                     /translation="QLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQS
                     TTVKDAAMQWGFWHLGQFATDYQQLFAEKPSLTLHQRMREWG"
     gene            complement(269..1168)
                     /gene="hemF"
                     /locus_tag="ON345_001178"
     CDS             complement(269..1168)
                     /gene="hemF"
                     /locus_tag="ON345_001178"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR3071271.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(1172..2041)
                     /gene="amiA"
                     /locus_tag="ON345_001179"
     CDS             complement(1172..2041)
                     /gene="amiA"
                     /locus_tag="ON345_001179"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR3071272.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            2255..2680
                     /locus_tag="ON345_001180"
     CDS             2255..2680
                     /locus_tag="ON345_001180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR3071273.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            2667..3116
                     /locus_tag="ON345_001181"
     CDS             2667..3116
                     /locus_tag="ON345_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR3071274.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            3323..3898
                     /locus_tag="ON345_001182"
     CDS             3323..3898
                     /locus_tag="ON345_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR3071275.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            3994..4893
                     /gene="yfeX"
                     /locus_tag="ON345_001183"
     CDS             3994..4893
                     /gene="yfeX"
                     /locus_tag="ON345_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR3071276.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEEMRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(4951..6255)
                     /gene="pbp4b"
                     /locus_tag="ON345_001184"
     CDS             complement(4951..6255)
                     /gene="pbp4b"
                     /locus_tag="ON345_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCR3071277.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDVGYPSVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDCYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDANFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(6260..7684)
                     /gene="murP"
                     /locus_tag="ON345_001185"
     CDS             complement(6260..7684)
                     /gene="murP"
                     /locus_tag="ON345_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR3071278.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMISAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(7688..8584)
                     /gene="murQ"
                     /locus_tag="ON345_001186"
     CDS             complement(7688..8584)
                     /gene="murQ"
                     /locus_tag="ON345_001186"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3071279.1"
                     /translation="MQLEKMITEGSNATSAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            8748..9605
                     /gene="murR"
                     /locus_tag="ON345_001187"
     CDS             8748..9605
                     /gene="murR"
                     /locus_tag="ON345_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR3071280.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            9734..10525
                     /gene="ucpA"
                     /locus_tag="ON345_001188"
     CDS             9734..10525
                     /gene="ucpA"
                     /locus_tag="ON345_001188"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR3071281.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            10829..11845
                     /gene="cysP"
                     /locus_tag="ON345_001189"
     CDS             10829..11845
                     /gene="cysP"
                     /locus_tag="ON345_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR3071282.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            11845..12678
                     /gene="cysT"
                     /locus_tag="ON345_001190"
     CDS             11845..12678
                     /gene="cysT"
                     /locus_tag="ON345_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR3071283.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQLVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            12678..13553
                     /gene="cysW"
                     /locus_tag="ON345_001191"
     CDS             12678..13553
                     /gene="cysW"
                     /locus_tag="ON345_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR3071284.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            13543..14640
                     /gene="cysA"
                     /locus_tag="ON345_001192"
     CDS             13543..14640
                     /gene="cysA"
                     /locus_tag="ON345_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR3071285.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PCRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            14774..15685
                     /gene="cysM"
                     /locus_tag="ON345_001193"
     CDS             14774..15685
                     /gene="cysM"
                     /locus_tag="ON345_001193"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR3071286.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(15688..16056)
                     /locus_tag="ON345_001194"
     CDS             complement(15688..16056)
                     /locus_tag="ON345_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR3071287.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            16161..17012
                     /gene="pdxK"
                     /locus_tag="ON345_001195"
                     /gene_synonym="thiJ"
     CDS             16161..17012
                     /gene="pdxK"
                     /locus_tag="ON345_001195"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR3071288.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(17054..17563)
                     /gene="crr"
                     /locus_tag="ON345_001196"
     CDS             complement(17054..17563)
                     /gene="crr"
                     /locus_tag="ON345_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3071289.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(17604..19331)
                     /gene="ptsI"
                     /locus_tag="ON345_001197"
     CDS             complement(17604..19331)
                     /gene="ptsI"
                     /locus_tag="ON345_001197"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR3071290.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(19376..19633)
                     /gene="ptsH"
                     /locus_tag="ON345_001198"
     CDS             complement(19376..19633)
                     /gene="ptsH"
                     /locus_tag="ON345_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR3071291.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(20017..20988)
                     /gene="cysK"
                     /locus_tag="ON345_001199"
     CDS             complement(20017..20988)
                     /gene="cysK"
                     /locus_tag="ON345_001199"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR3071292.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(21173..21934)
                     /gene="cysZ"
                     /locus_tag="ON345_001200"
     CDS             complement(21173..21934)
                     /gene="cysZ"
                     /locus_tag="ON345_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR3071293.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            22164..23150
                     /gene="zipA"
                     /locus_tag="ON345_001201"
     CDS             22164..23150
                     /gene="zipA"
                     /locus_tag="ON345_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR3071294.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            23221..25236
                     /gene="ligA"
                     /locus_tag="ON345_001202"
     CDS             23221..25236
                     /gene="ligA"
                     /locus_tag="ON345_001202"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR3071295.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLKQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVDVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKKSLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            25238..25456
                     /locus_tag="ON345_001203"
     CDS             25238..25456
                     /locus_tag="ON345_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR3071296.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25453..26451)
                     /locus_tag="ON345_001204"
     CDS             complement(25453..26451)
                     /locus_tag="ON345_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR3071297.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            26541..27467
                     /locus_tag="ON345_001205"
     CDS             26541..27467
                     /locus_tag="ON345_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3071298.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(27458..27799)
                     /locus_tag="ON345_001206"
     CDS             complement(27458..27799)
                     /locus_tag="ON345_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR3071299.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
BASE COUNT         6654 a         7017 c         7562 g         6648 t
ORIGIN      
        1 atcaactgca tgtcagtcgc cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg
       61 gcccaaacgc gtggctgaaa cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc
      121 cgtggtcgca aagcacaacg gtaaaagacg ccgccatgca gtggggattc tggcatctgg
      181 ggcaatttgc cacggattac cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc
      241 agcggatgcg ggaatggggg tgagggagtt acacccaatc cctgacctta ataaactcac
      301 ttaacgccgc ttctgggctg ccatcttttg gctgataatc atactcccag cgtaccagtg
      361 gcggcattga catcaggatc gactcggtgc gcccgccagt ttgcaggcca aacagagtgc
      421 cgcgatccca gaccagattg aactcgacat aacgaccgcg acggtagagc tgaaaattgc
      481 gctcgcgctc gccgtaggcc atcgctttac gtcgctctac aattggtaaa taagcgtcgg
      541 tgtagccttt gcctaccgcc tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca
      601 gatcatcaaa gaacagcccg ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt
      661 aatcgtcgca ccactttttg taacggggat aaacgtcttc accaaatggc aggcacaggt
      721 cacgggcggt gcggtgccag tgaatggcgt cttcttcaaa accatagaaa ggggttaaat
      781 cgaagccgcc gccaaaccac cagacgggct ctgcacccgg tttttcggca ataaaaaaac
      841 gcacattcgc gtggctggtg ggaacatacg ggttatgcgg atgcactacc agtgaaacgc
      901 ccatcgcctc gaaactgcgc ccggcaagct ccgggcgatg agcggtggcg gaagcaggca
      961 tcgcctcgcc gtggacgtga gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac
     1021 gcaacacccg actacgcccg ccgccgccag cttcgcgctg ccaactatct tcgacaaatt
     1081 ctgcgccatc gacggcggtc agctgctgac aaatcgtatc ctgaaggttg agcagaaact
     1141 gtttaacctg gtgtgcgtcg ggtttcataa cttatcgctt tttcgaatgt gctttctggt
     1201 tgtcgaacca gtggaaataa ctgatcacgc cttcagcaat cgctgtggcg attttctgac
     1261 gaaacgccgc cgtgcctaac agccgctctt cttccgggtt ggtgataaac gaggtttcca
     1321 ccagcaccga aggaaccgac ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc
     1381 ggctgtgcag tttatgcacc ggcttaatct ttttcagaat atgcgagcca agcgtcaggc
     1441 tgtttttaat agtatcggtt tgcaccagat caaacagcac ttgctgcaat aggtgatcct
     1501 tgtcagtcgc ctttttaccg gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt
     1561 tcgccattgc gctacttgcc ccacggttag agagggcaaa taccgaagca ccggcagctt
     1621 tcgggttggt aaagccatcg gcatgaattg acataaacag atctgcgcca tgtttatggg
     1681 cgatttcaac gcgatcgtaa agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat
     1741 caatcccatg attacgcaaa atggaacgga cgtttttagc aatcgccagc accacatgtt
     1801 tttctttcga accgttgcgt ccgatcgctc cggtatcaat tccgccgtgg cctggatcga
     1861 gaacaacgac acgtttgccg ccagattttt tggctttcgg cttgctgtgt ccgttgctgg
     1921 tttttaaagg ttcgtctttg gcgatggctt gcgacattcc tgacaacgtc agggcagcca
     1981 aaccggcttt cagcacctgg cggcgcgaag tgagtgtttt tagtggttta aaagtgctca
     2041 tacggcctga gttgtaataa taaagttcca gatgttatat cgtatcgcgt aatccgttac
     2101 gacggtttga tgttgagaat tgttttactt ttcatttcaa tagttgacaa ctccccatta
     2161 tgccactttt tcaaccgatt ttcgtcagat attggctaat tcgaccgttc acgccataat
     2221 cagcctttta acccgcaaaa aggtaacgaa taccatggag atacgcgtat ttcgccagga
     2281 agatttcgaa gaggtcatca ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga
     2341 tccggaaatg gacatcgagc gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga
     2401 ggtgaacggt gaagtggtcg gaacggtgat gggcggttat gacgggcatc gcgggtctgc
     2461 ttattatctt ggcgtgcatc cagagtttcg tgggcgtggg attgccaatg cgttgcttaa
     2521 tcggctggag aaaaagctga ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga
     2581 agataacgac atggtgctcg gaatgtatga acgcctggga tatgaacacg ccgacgtgct
     2641 gagtctgggt aagcgtttga ttgaagatga agagtactga gttccatcct gtccattatg
     2701 atgcgcacgg acgcctgcgt ttacccttgc tcttctggct tgtgctattg cttcaggcgc
     2761 gaacctgggt gctgtttgtc attgccggtg cgtcgcgcga gcagggtact gcgctgctaa
     2821 atctgtttta tcccgatcac gataatttct ggctggggtt gattcccggt attccggcgg
     2881 tgctggcgtt tttgttgagc ggtcggcggg cttcgtttcc tcgcacctgg catgtgctct
     2941 attttctgtt gctgttggcg caggtggttt tactttgctg gcaaccgtgg ctgtggctga
     3001 acggtgaatc cgttagcggt atcggtctgg cgctggtagt ggcggatatt gtggcgttaa
     3061 tctggctgct gaccaatcga cgtttacgcg cttgttttaa tgaagaaaaa gaataacgca
     3121 gaggtgcgct ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaacatc
     3181 gaaaattcaa tatattgcag gagaaagtag gcctgataag cgtagcgcat caggcaattt
     3241 tgtgtttgtc atcaaccttt aacggcactt tttggtgaat ttgcactcca agcaacgtta
     3301 ttgaataacc aaaggaagtg acatgaaatc gctgcgttta atgttatgcg ctatgccgtt
     3361 gatgctgacc ggctgttcca cgatgtcgtc agttaactgg tcggccgcta acccgtggaa
     3421 ctggtttgga tcgtccacca aagtgagcga gcagggcgtg ggtgaattaa cggcgtccac
     3481 accactgcaa gaacaagcca ttgctgatgc gcttgatggc gattatcgcc tgcgcagcgg
     3541 aatgaaaacc gcgaacggca acgtggtgcg cttttttgaa gtgatgaaag gcgacaacgt
     3601 ggcgatggtg attaacggcg atcaaggcac gatcagtcgc attgacgtgc tggatagcga
     3661 tattcctgct gacaccggtg ttaaaatcgg tacaccgttt agcgaccttt acagcaaagc
     3721 atttggcaat tgccaaaaag ctgatggtga tgataatcgt gccgtcgaat gtaaagccga
     3781 aggcagtcaa catattagct accagttcag cggggaatgg cgtggtcctg aagggttaat
     3841 gccttcggac gataccctga aaaactggaa agtcagtaaa attatctggc ggcgttaatt
     3901 tgcgtctgaa caaaccgccg ccgcgaaaaa acacgtaaaa tagcgctcaa caatgccacg
     3961 gattgcgtgg cattcttatt ttcaggagga acaatgtctc aggttcagag tggcattttg
     4021 ccagaacatt gccgcgcggc gatttggatc gaagccaacg tgaaagggga agttgacgcc
     4081 ctgcgtgcgg ccagtaaaac atttgccgac aaactggcaa cttttgaagc gaaattcccg
     4141 gacgcgcatc ttggtgcggt ggttgccttt ggtaacaaca cctggcgcgc tctgagcggc
     4201 ggcgttgggg ctgaagagct gaaagatttt ccgggctacg gtaaaggcct tgcgccgacc
     4261 acccagttcg atgtgttgat ccacattctt tctctgcgtc acgacgtaaa cttctctgtc
     4321 gcccaggcgg cgatggaagc ctttggtgac tgcattgaag tgaaagaaga gatccacggc
     4381 ttccgttggg tggaagagcg tgacctgagc ggctttgttg acggtacaga aaacccggcg
     4441 ggtgaagaga tgcgtcgcga agtggcggtt atcaaagacg gcgtggatgc cggcggcagc
     4501 tatgtgtttg tgcagcgttg ggagcacaac ctgaagcagc tcaaccggat gagcgttcac
     4561 gatcaggaga tgatgatcgg gcgcaccaaa gaggccaacg aagagattga cggcgacgaa
     4621 cgtccggaaa cctctcacct cacccgcgtt gatctgaaag aagatggcaa agggctgaag
     4681 attgttcgcc agagcctgcc gtacggcacc gccagtggca ctcacggtct gtacttctgc
     4741 gcctactgcg cgcgtctgca taacattgag cagcaactgc tgagcatgtt tggcgatacc
     4801 gatggtaagc gtgatgcgat gttgcgtttc accaaaccgg taaccggcgg ctattacttc
     4861 gcgccgtcgc tggacaagct gatggcgctg taagcgtttc tgattctatg attaaaggcc
     4921 agccagtaac aaaactgact ggccttttta ttacttttgc ttcaacgccg catacacctg
     4981 atcaactacc caaccataag ttgcaatcgg taactgaccg ctttcgaaca tattgggatt
     5041 tttttgtgga tcggcaactg gcgaatgggg cttgttgctt aacatcacaa tcgccatatg
     5101 attgaccgga tcgataacgg ttactgtccc ggtccagcca gtgtgaccgt aggtttgcgg
     5161 gcttgccagc gtgccaaacg tcggcgtcat ggtggcatta ccattcacgc gccagccgag
     5221 gccaaaattg gcatcttcct tagagctggt ggtaaacatc ttcaccgttt ccgcactgaa
     5281 cagctgcaca tcgccatagc ccccgccgtt cagcatcgtt tgcattaaca ccgcaatatc
     5341 gccggtattg gaaaacaaac ctgcgtgccc ggaaacgccg cccatcgaat aaaaggcttt
     5401 ttcatcgtgc acctgacccc agagagtgga ggtgcggata ttcgggaaat ggatcacgcc
     5461 atcgcgggta ttgccgttca attccgtggc ggcaatttgt tgcggtttaa agcctttcag
     5521 cagcgggtta aataccgtat gtgtcaggcc gagcgggcga taaatcgact cttcaacata
     5581 gcagtcaagc ggctgaccgg tgaccgactc gacgataaat ccaagcagca tataatcgac
     5641 atcactgtaa atatgcttgc tgccgggctg atactctagc ggcgtgcgtt tgatcatctc
     5701 cagcgtctgg cctttatcct gggaatataa cgcgcccgcg accgctttat tcgggtattg
     5761 cggatctgcc gggaaaccgc cgctgtgatg cagcaggtca gaaatccgca gggtgttttt
     5821 gcctttaatg gtgtcatttg gactatcggc aaaccccgga atatatttcg caatccgatc
     5881 gtcaggatgc agtttgcctt cgctcatcag cttttgcagg gcgaagttcg tggcgtacat
     5941 tttggtgttt gaggccaggt catacagcgt cccggtggtg gctttgacag gctgttccat
     6001 caacacgctg ccatcgtact ttttcgccgc tccccaggct ttgcgataca caatctgatt
     6061 atctttaatg atcagcaggt ttacgctggg ataaccgaca tcaacttgct ggctaatcca
     6121 gcgatccatc tggttaagcc gttcgacgtt aaaccctgct ttctctggcg agctttctgt
     6181 cagaacaggg tattttgcgg cgctgacgct acaactaaca gccagcagag aaagatagag
     6241 cattgtccgt ttcatgatat cagtccagat tgacgttacg gcagccaaag agcgtggtga
     6301 aaataaaccc gcaaacccat gccaccagaa tcccgccagc ataaaccgcc atggccggaa
     6361 ggatgccttg tgcggaaatc atcagcggca gcgctaccag accagacggc ccaaaggcgc
     6421 tgtttaagcc catcggcaga ccccaccagg ctatcaggcc gataaacaaa ccgcccgccg
     6481 cgccgcctaa acaggcggta acaaacggct tcatgcgggg cagggtgaca ccgtaaatca
     6541 gcggttcacc aacgcccagc aggccgggaa taatcgcccc gcgtacctga ctgcgtagcg
     6601 cactgtgcgg ttgcgcccgc cagtagagtg ccagcgccgc gcccacctgg cccgcgcctg
     6661 ccattgaaag gatgggaaat aagctgttga atccctggct gtccattaac gcgaggtaaa
     6721 caggaataaa gccctgatgc acgccaaata ccacggcgat caggaacagc ccggctaaaa
     6781 ccgcacaacc gaacggatta ctgttcaggt gcataaacag ccatgacata ccttcgaata
     6841 accagccacc cagcggcata atgatcaggt acgcaagcgt agcggtgatc agcagggtga
     6901 ttaacgaggt cagcagcatg tcgagatcgt ccggcataaa gcggcgcacc atgccttcaa
     6961 tgcgggcgca ggcccacgcg gcaatcagca cgccgataat attgccgcgc ggatcgatgg
     7021 gcagaccaaa gaaatcgtga aaaccggcgt agtaaccggt ggttgcagcg gggttataac
     7081 cgagcaaaaa gagtgcggca ataattgcgc cattgacgcc cgtgccgcca aatgcctggg
     7141 cggcgttata gcccaccaga atcaccagga aagtgaacaa acctttgctg aacaccttca
     7201 taaaattcag cgcgtcaggt agcgtgccct gagcatctgc cggaacgtgc atcaccgtgg
     7261 caattaacgt cgctatcccc agcagcagac cggcggcaat aaaaccgggg atcagcggcg
     7321 tgaagatggt ggcgaatttg gcgagaaatt gttgcacgcc ggaggtttgt ttggctttta
     7381 actgacgttt gttttgggcg gcgatttctg cggcatcctg taccggtgcc tcgcccagca
     7441 gctcgctcat cgcttttgcg gcacgatgcg ctttaccagg cccaaaaacg acctgcacct
     7501 ggtcgctggt caaaatgacg cccttcacgc cttccagcgt tttgatattg ggatcaacca
     7561 gtgaactgtc atgtacaccc agacgcaggc gcgtcataca gttaccacaa ctggcgatat
     7621 ttcccggtcc gccgacacgg gtaagaatgg tgttcagaag ttcactgctg atctctttgg
     7681 ccatgggtta ttccttgtct aaaacctgac gaataaagcc gccgtgttga tccaggcgtt
     7741 ttttagcttc tgcggcatcg agatttttca gcaccatcac aatggccgtt ttacagttgc
     7801 gctcgcaggc aattaacgcc gcttccgctt gttcggcatt acatccggtg gcgtttttaa
     7861 cgatattgac ctggcgcaca tgcagttttt cgttggtggc gaccacatcg accatcaggt
     7921 tgccgaacac tttgccggat ttaatcatca gcccggtgga aagcatattg agcaccagtt
     7981 tctgcgctgt gcctgctttc attcgcgaag aaccagtgac cacttcagca cctacaatcg
     8041 gtgtaatggc aaactcggcg gtggtggaaa cggcactccc cggattacag gaaattccca
     8101 ctgtgcggca accgagctgg cgggcgtatt ccagtccggc aatcacatac ggcgtacgac
     8161 cgctggcggc aataccgacc accacatcct gcgcggttaa gccgatgttt ttcaaatcat
     8221 taacaccgcc ttcccggcta tcttccgcgc cttccaccgc gtgctgaatg gcatattcgc
     8281 cgccagcaat caaaccaacc accagacccg gtttcacgcc gtaggtgggc ggacattcgc
     8341 tggcatccag aatccccaga cgaccggatg ttcccgcacc gaggtaaatc agacgcccgc
     8401 cgccgcttac ctgggcgtgg ataacatcga tcgccgcggc gatatccggc agtacgcgct
     8461 caacggcaag cggtacggtt ttatcttcat cgttgataat ccggcacatt tccagcgttg
     8521 atacgcggtc aatttcagcc gaggtggcgt tcgagccttc agtaatcatc ttttcaagtt
     8581 gcatcggtgg tgaccttact atttacgatc aaataattgc agatgtatgg aatcatatat
     8641 cccgaattga cagacttaaa ggaatcattg agtctacact cgcagagtgt gaagagcgtc
     8701 ataagaactt aagcggaatt tttgctgcca gcccgggaga gtggaagatg ttgtacctga
     8761 cgaaaatacg caatgcggaa agtgaattta ccgagaacga gcaaaaaatt gctgactttc
     8821 tgcgggccaa cgtgagtgag ctgaaatccg tctcttcgcg caagatggcg aaacagctcg
     8881 gcatcagtca gtcgagcatt gtgaaatttg cccagaagct cggcgcgcaa ggatttactg
     8941 aattgcgtat ggcgttaatt ggcgaataca gtgccagccg ggagaaaacg aatgccacgg
     9001 cgctgcacct gcacagttcg attaccagtg atgattcgct ggaagtgatt gcccgcaaac
     9061 tgaatcgcga aaaagagctg gcgctggagc aaacctgtgc gttgttcgac tacgcgcggc
     9121 tgcaaaaaat cattgaggtc atcagtaaag caccgtttat ccagataacg ggcctgggcg
     9181 ggtcggcgct ggtggggcgc gatctgtcgt tcaaactgat gaaaattggc tatcgcgtgg
     9241 cctgtgaagc cgacacgcac gtacaagcca ccgtttccca ggcgttgaaa aaaggcgatg
     9301 tacaaatcgc tatctcttac agcggcagta aaaaagagat tgtcttgtgc gcagaagcgg
     9361 cacgaaagca gggggcaacg gtgattgcca ttacttctct ggctgactct ccgctgcggc
     9421 ggctggcgca tttcacgctc gataccgttt ccggtgaaac cgaatggcgt agctcgtcga
     9481 tgtctacccg cacggcgcaa aattctgtta ccgatttact gtttgttggt cttgtgcaac
     9541 tcaatgatgt ggagtcattg aaaatgattc agcgcagcag tgagctaaca caacgcctga
     9601 aatgatgcat aaagcagcga ctggattgag attttcctga attagtgagc tgatccgcag
     9661 caatattttg tttatcctgt attttcagag ggaatggagt gtaacgctct gtattaacaa
     9721 ggagagcatt aaaatgggta aactcacggg caagacagca ctgattacgg gcgcattgca
     9781 gggaattggc gaaggaattg ccagaacttt tgcgcgtcat ggcgcaaacc taatcttgct
     9841 ggatatctcc cctgagatcg aaaagctggc ggacgaactg tgtggtcgtg gtcatcgctg
     9901 tacggcggtt gtcgccgatg tgcgtgaccc ggcgtcggta gccgcagcta tcaaacgcgc
     9961 gaaggaaaaa gaagggcgca ttgatatcct ggtgaataac gcaggcgttt gtcgtctggg
    10021 cagtttcctc gatatgagcg atgacgatcg cgatttccat attgacatca atattaaagg
    10081 cgtatggaac gtcacgaagg cggtgctgcc ggagatgatt gcccgcaaag atggtcgcat
    10141 tgtgatgatg tcttcagtca ctggtgatat ggtggccgat cctggcgaaa cggcgtacgc
    10201 cttaacgaaa gcggcgattg ttggcctgac aaaatcgctg gcggtggagt acgcgcagtc
    10261 tggtattcgc gttaacgcca tttgcccggg atacgtgcgc acaccaatgg cggaaagcat
    10321 tgcccgccag tcgaacccgg aagatccaga gtcggtgctg actgaaatgg cgaaagcaat
    10381 cccgatgcgt cgcctcgccg atccgctgga agtcggcgaa ctggcggcct tcctcgcatc
    10441 ggatgaatcc agctatttaa ccggtacaca gaatgtgatt gatggcggca gcacactgcc
    10501 ggagacggtt agcgtcggta tctgattcac ctctgtttcc tccctgcatt tgtggggagg
    10561 atttcgtctt gaactatgtt caccaggcta ttttatttgt cattttggcc ccgggcagtg
    10621 ctcgaaatcc tcacgtacta tgtgtacgct ccggtttctc cgcgctgttc gtgtccaaac
    10681 tgactgcaac aattacgcct gttgaaccaa gttcttattc ccttttcaac ttccaaatca
    10741 ccaaacggta tataaaaccg ttactccttt cacgtccgtt ataaatatga tggctattag
    10801 aaagtcatta aatttataag ggtgcgcaat ggccgttaac ttactgaaaa agaactcact
    10861 cgcgctggtc gcttctctgc tgctggcggg ccatgtacag gcaacggaac tgctgaacag
    10921 ttcttatgac gtctcccgcg agctgtttgc cgccctgaat ccgccgtttg agcaacaatg
    10981 ggcaaaagat aacggcggcg acaaactgac gataaaacaa tctcatgccg ggtcatcaaa
    11041 acaggcgctg gcgattttac agggcttaaa agccgacgtt gtcacttata accaggtgac
    11101 cgacgtacaa atcctgcacg ataaaggcaa gctgatcccg gccgactggc agtcgcgcct
    11161 gccgaataat agctcgccgt tctactccac catgggcttc ctggtgcgta agggtaaccc
    11221 gaagaatatc cacgattgga acgacctggt gcgctccgac gtgaagctga ttttcccgaa
    11281 cccgaaaacg tcgggtaacg cgcgttatac ctatctggcg gcatggggcg cagcggataa
    11341 agctgacggt ggtgacaaag gcaaaaccga acagtttatg acccagttcc tgaaaaacgt
    11401 tgaagtgttc gatactggcg gtcgtggcgc gaccaccact tttgccgagc gcggcctggg
    11461 cgatgtgctg attagcttcg aatcggaagt gaacaacatc cgtaaacagt atgaagcgca
    11521 gggctttgaa gtggtgattc cgaaaaccaa cattctggcg gaattcccgg tggcgtgggt
    11581 tgataaaaac gtgcaggcca acggtacgga aaaagccgcc aaagcctatc tgaactggct
    11641 ctatagcccg caggcgcaaa ccatcatcac cgactattac taccgcgtga ataacccgga
    11701 ggtgatggac aaactgaaag acaaattccc gcagaccgag ctgttccgcg tggaagacaa
    11761 atttggctcc tggccggaag tgatgaaaac ccacttcacc agcggcggcg agttagacaa
    11821 gctgttagcg gcggggcgta actgatgttt gctgtctcct ccagacgcgt gctgccgggc
    11881 tttaccttaa gcctcggcac cagtctgctg tttgtgtgcc tgattttgct gctgccgctc
    11941 tccgcgctgg tgatgcaact ggcccagatg agctgggcgc agtactggga ggtgatcacc
    12001 aacccgcagc tggtcgcggc ctacaaagta acgctgctgt cggcgtttgt ggcatcgatt
    12061 tttaacggcg ttttcggtct gctgatggcg tggatcctaa cccgctatcg cttcccaggc
    12121 cgcacgctgc ttgatgcgct gatggattta ccctttgcgc tgccaacggc tgtcgccggt
    12181 ttaacgctgg cctcgctctt ttccgtaaac ggtttttacg gtgaatggct ggcgaagttt
    12241 gatatcaaag tcacctatac ctggctgggg attgcggtgg cgatggcctt taccagcatt
    12301 ccgtttgtgg tgcgtaccgt gcagccggtg ctggaagagt taggcccgga atatgaagaa
    12361 gcggcggaaa cgcttggtgc aacgcgctgg cagagtttct gcaaagtggt gctgccggag
    12421 ctttctccgg cgctggtggc gggcgtggcg ctgtcgttta cccgtagtct tggtgaattt
    12481 ggcgcggtga tttttatcgc cggaaatatc gcgtggaaga cggaagtgac gtcgctgatg
    12541 atttttgtgc gcttacagga gtttgattac ccggcagcga gcgcgattgc ttcggtgatc
    12601 ctcgcggcat ctctgctgct gctgttctca attaacactc tgcaaagtcg ctttggtcgg
    12661 cgtgtggtag gtcattaatg gcggaagtta cccaattgaa gcgttatgac gcgcgcccga
    12721 ttaactgggg caaatggttt ctgattggca tcgggatgct ggtttcggcg ttcatcctgc
    12781 tggtgccgat gatttacatc ttcgtgcagg cattcagcaa ggggctgatg ccggttttac
    12841 agaatctggc cgatccggac atgctgcacg ccatctggct gacggtgatg atcgcgctga
    12901 ttgccgtacc ggtaaacctg gtgttcggca ttctgctggc ctggctggtg acgcgcttta
    12961 acttccctgg acgccagtta ctgctgacgc tactggacat tccgtttgcc gtatcgccgg
    13021 tggttgccgg tctggtgtat ctgctgttct acggctctaa cggcccgctg ggcggctggc
    13081 tggatgaaca taatctgcaa attatgttct cctggcccgg aatggtgctg gtcaccatct
    13141 ttgtgacgtg tccgtttgtg gtgcgcgagc tggtgccggt gatgttaagc cagggcagcc
    13201 aggaagacga agcggcgatt ttgcttggcg cgtctggctg gcagatgttt cgccgcgtca
    13261 cattgccgaa cattcgctgg gcgctgcttt atggcgtggt gttgaccaac gcccgcgcaa
    13321 ttggcgagtt tggcgcggtg tcggtggttt ccggctcgat tcgcggcgaa accctgtcgc
    13381 tgccgttaca gattgaattg ctggagcagg actacaacac cgtcggctcc tttaccgctg
    13441 cggcactgtt aacgctgatg gcgattatca ccctgttttt aaaaagtatg ttgcagtggc
    13501 gcctggagaa tcaggaaaaa cgcgcacagc aggaggaaca tcatgagcat tgagattgcc
    13561 aatattaaga agtcgtttgg tcgcacccag gtgctgaacg atatctcact ggatattcct
    13621 tcaggtcaga tggtcgcgtt gctggggccg tccggttccg ggaaaaccac gctgctgcgc
    13681 attatcgccg ggctggagca tcaaaccagc gggcatattc gcttccacgg caccgacgtc
    13741 agccgcctgc acgcgcgcga tcgcaaggtc ggtttcgtgt tccagcatta cgcgctgttc
    13801 cgccatatga cggtattcga caatatcgct tttggcctga cggtgctgcc gtgtcgcgat
    13861 cgcccgaatg ccgcggcgat caaagcgaaa gtgacaaaat tgctggagat ggtgcagctt
    13921 gctcatctgg cggatcgtta tccggcacag ctttccggcg gccagaaaca gcgcgtggcg
    13981 ctggcgcgcg cgctggctgt agaaccgcaa attctgctgc ttgatgaacc gtttggcgcg
    14041 ctggatgcgc aggtgcgtaa agagctgcgt cgctggctgc gtcaactgca tgaagagctg
    14101 aaattcacca gcgtgttcgt gacccacgac caggaagaag cgacggaagt agctgatcgt
    14161 gtagtggtga tgagccaggg caatattgaa caggctgatg cgccggagca ggtatggcgc
    14221 gaaccagcga cccgttttgt gctcgaattt atgggcgaag tgaaccgcct gcagggaacc
    14281 attcgcggcg ggcagttcca tgttggcgca catcgctggc cgctgggcta cacacctgct
    14341 tatcaggggc cggtggatct cttcctgcgc ccgtgggaag tggatatcag ccgccgtacc
    14401 agtctcgatt cgccgctgcc ggtacaggta ctggaagcca gcccgaaagg tcactacacc
    14461 caattagtgg tgcagccgct ggggtggtac aacgaaccgc tgacggtcgt gatgcatggc
    14521 gacgatgccc cgcagcgtgg cgagcgttta ttcgttggtc tgcaacatgc gcggctgtat
    14581 aacggcgacg agcgtatcga aacccgcgat gaggaacttg ctctcgcaca aagcgcctga
    14641 taggttgagt gaatgttaaa cgcccggagg cgtttcccgc gatccgggct ttttaatggc
    14701 aaggtttgta acctgtagac ctgataagac gcgcaagcgt cgcatcaggc aacaccacgt
    14761 atggatagag atcgtgagta cattagaaca aacaataggc aatacgcctc tggtgaagtt
    14821 gcagcgaatg gggccggata acggcagtga agtgtggtta aaactggaag gcaataaccc
    14881 ggcaggttcg gtgaaagatc gtgcggcact ttcgatgatc gtcgaggcgg aaaagcgcgg
    14941 ggaaattaaa ccgggtgatg tcttaatcga agccaccagt ggtaacaccg gcattgcgct
    15001 ggcaatgatt gccgcgctga aaggctatcg catgaaattg ctgatgcccg acaacatgag
    15061 ccaggaacgc cgtgcggcga tgcgtgctta tggtgcggaa ctgattcttg tcaccaaaga
    15121 gcagggcatg gaaggtgcgc gcgatctggc gctggagatg gcgaatcgtg gcgaaggaaa
    15181 gctgctcgat cagttcaata atcccgataa cccttacgcc cattacacca ctaccgggcc
    15241 ggaaatctgg cagcaaaccg gcgggcgcat tacccatttt gtctccagca tgggaacaac
    15301 cggcactatc accggcgttt cacgctttat gcgcgaacaa tccaaaccag taacgattgt
    15361 tggcctgcag ccggaagaag gcagcagtat tcccggcatt cgccgctggc ctgcggaata
    15421 tctgccgggg attttcaacg cttctctggt ggatgaggtg ctggatattc atcagcgcga
    15481 tgcagaaaac accatgcgcg aactggcagt gcgggaagga atattctgtg gcgtcagctc
    15541 cggcggcgcg gttgccggag cactgcgggt ggcaaaagct aaccctggtg cggtggtggt
    15601 agcgatcatc tgcgatcgtg gcgatcgcta cctttccacc ggggtgtttg gggaagagca
    15661 ttttagccag ggggcgggga tttaagatta ttgcgcaggc accgttttat ccgtctgcgc
    15721 aattagcgca tgtaaaaaag gctgtgcgtt ctcatcgctc ttaccgggga tcttcacaat
    15781 atacggcttc ccggtaatcg atgacgacga agcaacctta tcaataaact gctcggcagt
    15841 gtcaatgcgg ttacgggtat tgcccagctt cagacgcaga tgagaaaccg cttcatcgca
    15901 ggtatgttca tcaccgttgc gcacaaatat caaatccttt ttctgtgcta atccctccag
    15961 catggcgttg atgcgggctt cttcatgggc agttaacttc gcgtaagcgg ggagtgtcat
    16021 tagtagtgta atgaccagac aaatgatttt cttcattgaa ttaccttatc cttgttttgt
    16081 gatagccgat ttgactgcca gaatcaggta aagtttcggt tggaatacga tgaggatcat
    16141 cggcaaggag aaaaaattaa atgagtagtt tgttgttgtt taacgataag agcagggcgc
    16201 tgcaggcgga tatcgtcgcc gtgcagtcgc aggtggttta cggcagcgtg ggcaacagca
    16261 ttgccgtgcc tgctatcaaa cagaacggtc tgaatgtctt tgccgtgccg acggtattgc
    16321 tgagcaatac gccgcattat gacactttct acggtggtgc gattccggac gaatggttta
    16381 gcggctattt gcgtgcgctt caggagcgtg atgcgctgcg ccaacttcgt gctgtaacca
    16441 cgggctatat gggaacggca tcgcaaatca aaatccttgc cgagtggctg actgcgctac
    16501 gcaaagacca tcctgaccta ttgatcatgg tcgatccggt gattggcgat attgatagcg
    16561 gaatttatgt caaacctgac cttcccgagg cgtatcgaca atatttactg ccgctggcgc
    16621 agggaattac ccccaatatc tttgagctgg aaatcctgac cggtaaaaat tgccgcgatc
    16681 tcgacagtgc cattgctgcc gcaaaaagtc tgctttcaga cacattaaaa tgggtggtga
    16741 ttaccagcgc ctccggtaat gaagaaaatc aggagatgca ggtggtggtg gtcagtgccg
    16801 acagcgtgaa tgtcatttcc cattcacggg taaaaaccga cctgaaaggg actggcgacc
    16861 tgttttgtgc tcagcttatc agtggtttgc tgaaagggaa ggcgttaaac gatgcggtgc
    16921 accgtgcggg gttgcgcgta ctggaagtga tgcgctacac ccagcagcat gagagcgatg
    16981 aattgatttt gccgccgctg gcggaagcat aaaaaatggc gccgatgggc gccatttttc
    17041 actgcggcaa gaattacttc ttgatgcgga taaccggggt ttcacccacg gttacgctac
    17101 cggacagttt gatcagttct ttgatttcgt ccatgttgga gataacaacc ggagtcaggg
    17161 tagacttggc tttctcttcc agcagcggca gatcaaattc aatgacagta tcgccaactt
    17221 tcacgcgctg accttcttca gcaatacgct tgaagccttc gcctttcagt tcaacggtgt
    17281 cgataccgaa gtggacgaac agttcaacgc cgctatcaga ttcgatagag aacgcgtgat
    17341 tggtttcaaa gattttacca atggtaccgt ctacaggcgc gaccattttg ttacccgttg
    17401 gtttgatagc aataccatca ccaacgattt tttccgcaaa aacgacatcc ggcacgtctt
    17461 cgatattgac gatctcgcca gagagcggag caatgatctc aatagttccg gtatccttct
    17521 tgtcgtcgga aaccagagat ttcagtttat cgaacaaacc catgatcttc tcctaagcag
    17581 taaattgggc cgcatctcgt ggattagcag attgtttttt cttcaatgaa cttgttaacc
    17641 agcgtcatta actcgtccgt tgtcggttga gcaagagcct gctctgctaa caccttcgca
    17701 tcttcgaagt tcgtgttacg gataatcttc ttaatgcgcg ggatagaaat ggcgctcata
    17761 gagaattcgt ccagacccat ccccagcaac agaagtgtag cacgttcatc gccagcaagc
    17821 tcaccacaca tgccagtcca tttgccttca gcatgagaag catcaataac ttgcttgatc
    17881 aagttcagca cggacggtga cattggctgg taaaggtgtg aaatcatatc attaccacgg
    17941 tcaactgcca gagtgtactg cgttaaatca ttggtgccga tactaaagaa atcaacttct
    18001 ttggctaaat gacgtgcaat tgttgcggca gccggtgttt ccaccattac gccgatttca
    18061 attgactcgt caaacgcttt accttcgtcg cgcagttcct gtttgtagat ttcgatctct
    18121 ttgcgcagtg cacgcacttc ttcaacagag atgatcatcg ggaacataat gcgcaattta
    18181 ccgaaagccg aggcacgcag gatagcgcgg agctgatcgc gcaggatctc tttacgatcc
    18241 atcgcgatac ggatagcgcg ccagccgagg aacgggttct cttctttcgg gaagttcatg
    18301 tatggcagct ctttgtcgcc gccgatgtcc atggtacgaa cgataaccgc ctgcgagcca
    18361 cacgcttcag ccactgcttt gtaagcagca aactgttctt cttcagtggg cagcgcgtcg
    18421 cggtccatga acaggaactc agtacgatac agaccaacgc cttcagcgcc gttacgctct
    18481 gcaccttcaa cgtcacgaac cgtaccaatg ttagcgcata cttctacctg gtgaccgtcc
    18541 agcgtaatag ctggcagatc tttcagttta gcaagctctg ctttttcaga agccacttgc
    18601 tcctgaacag cgcgcatttt atcaataact tcgttggttg gattgacgta aacctgatta
    18661 tttacggcat ccagaatcag atagtcgtca tttttcacct gagaggtgac gctaccggta
    18721 cccacgatag caggcagttc cagagaacgc gccatgatag aggtgtggga agtacggcca
    18781 cccgcgtcgg tgatgaaacc cagcaccttc ttcaggttca gctgtgcggt ttcggacggc
    18841 gtcaggtcag cggcaaccag aatgacttca tcctgaatgg cgctcaggtc gataatcttc
    18901 aggcccagga tgttgcgcag caggcgctta ccgatatcac gtacgtcagc cgcacgttct
    18961 ttcaggtatt catcatccag ctcttccagg gcagaagcct gaccttcgat aacttcatga
    19021 gcagctgcgt cagctgtcat gtgtttatct ttaatcaggg ctatgatttc ctgctccagc
    19081 tcctcatctt cgagcagcat aatatgccct tcaaagatgg cttctttttc ttcaccgaac
    19141 gtttcaccag ctttcgtttt gatcgtttcc agctgggctg atgccttggc acgaccgctc
    19201 agaaaacgtt caacttcctg atcaacctgg tcggcagaaa tttttttccg gtcaatgaca
    19261 atttcgtctt ctttcagaag cagagcttta ccgaaagcga tacccgggga tgctaaaatg
    19321 cctgaaatca taaccctacc ttacttgtga ctgattttta aaagaacccg ggaaattact
    19381 cgagttccgc catcagttta accagatgtt caaccgcttt ctgctcgtct tcgccttctg
    19441 cggagatagt cacaacggta ccttgagtca ggcccagagt ctgcagttta aacaggcttt
    19501 tcgcgctggc gcttttgccg ttggaagtca cagtaatttc agaagtgaag cccttagctt
    19561 cttttacaaa ctgggcagca gggcgggtgt gcagaccgtt cggagcggta atggtaactt
    19621 cttgctggaa cattgtattt ccccaactta taggtttagt gttgtggaac taaagtctag
    19681 cctggcggtt cgactttagc ctgtattgtt agcgccagtt tttaacagac gcgacgcacg
    19741 aagagcgcaa tacagtccat tgctggcggg tgttgaccgc tgacgtttcg tacgtcatta
    19801 aacattatgc cgctaaagga agaattgaac caaatcataa aatcgattca gctttggctt
    19861 ttcctgtaac gattaatttc gcgcatcaaa ataattagtc tggttaaata ccagttccga
    19921 caggctgaat caatgccagg aggggcgaaa gtttgaagca ggccacaaaa aagcacctaa
    19981 aaaggtgctt ttttacgcat ttttaacaag ctggcattac tgttgcaatt ctttctcagt
    20041 gaagagatcg gcaaacaatg cggtgcttaa ataacgctca cccgatgatg gtagaataac
    20101 cacaatattc ttgttggtaa agctttcatc ttcttgtagt ttcaacgccg cggcaacagc
    20161 tgctccagaa gagatacctg caagaatacc ttcttcttcc attagacgac gggcggtaga
    20221 aatggcttct tcattggtga tgccaatgac tttatcgacc agcttgagat cgaggttagc
    20281 cgggataaaa ccagcgccaa taccctgaat tttatgcggg ccaggtttaa tctcttcacc
    20341 tgccagcgcc tgggcgataa ctggagaatc ggttggctca acggcgacag agataagatc
    20401 ggtcttgcct ttggtgcctt taatgtagcg gctgacgcca gtcagcgtac cgccagtccc
    20461 aacgccagca ataaatacat caacctgacc gtcggtatct tcccagatct ccggaccggt
    20521 ggttttttcg tgaatttcag ggtttgccgg attgctgaat tgttgcagca gcaggtattt
    20581 ctctggattg ctggcgacaa tttcttctgc tttttggatt gcgcctttca tgcctttagc
    20641 accttccgtc agcaccaggt ttgcacctaa cgctttcagc agcttgcggc gttcaatact
    20701 catggtttct ggcatggtca gggtgagttt gtaaccgcga gcggcagcta cataggccag
    20761 tgcaatcccg gtattaccgc tggtcggttc aaccagttca acgcctggtt tcagcacgcc
    20821 gcgcttttcg gcatcccaaa tcatgttggc accgatacgg cacttaacgc tgaagctggg
    20881 gttacgagat tccaccttcg ccagaatgcg tccgttaccg atgcgattca ggcgaaccag
    20941 cggcgtgtga ccgatagtca gcgagttatc ttcaaaaatc ttactcatgg cctgtcctta
    21001 actgtatgaa attgggatac aacaggtagc atacccgctc agagaatatg cggaagtaag
    21061 gatttagcat atctatatac agaagggaaa taatgacatg caagatggaa taaggggcgg
    21121 cataagccac ccctgtttca cacaaacggt ttacaaaata accggtagat tgttaccgcc
    21181 acatcgcgtg tttatcgcga tagcaatcga cccacatcgc cgtcgcgcca caaacggcaa
    21241 cgggcatgat gaacagatta agcagcggga tcatcgtaaa caggctggtt aaagcaccaa
    21301 actgcatatt ggtgattttg cgtgtgcgca gggcggtgcg catctcttta aacggcactt
    21361 tgtggttatc gaaggggtaa tcgcaatact ggatggctaa catccaggcg ctaaacagga
    21421 accacagtac cggcgcgacg gtttgcccaa taccagggat gaagtaaaga attagtaata
    21481 caattgcgcg cggcagatac caggcaaatt tttgccattc gcgtttcatg attcgcggca
    21541 catctttcat gataccgaaa atcccggtat ctggcggtgt agcgccagtc aatcgtgctt
    21601 ccagttgttc agccaataaa ccgttaaacg gagcggcaat ccagttagca atcgtggaga
    21661 agaaatagcc aaacactaac agcacagaga tgacagccag aggccacaac agataactca
    21721 gccattgtag ccagtccgga acgtaactca tgagagtcgg gatccagaca tcgagctgtg
    21781 taaagagcca ccagaatgcg ccccccatca acaaaatatt gaccagcagc ggtaaaataa
    21841 cgaaacgccg aatcccaggt tgcgagacga gcttccagcc ttgcgcaaaa tagtaaaaac
    21901 cgctgcgtgg ggcagatgtg aatgatgaaa ccataatcag gatgagctcc ttttgaccaa
    21961 tcccaggaaa attctacgta ttttaccggg taattgcgca atggacagtt aggatatgtt
    22021 cgaaaaaaca gcaaaaagca cgatttcatc tatctttgtg ctgtgaaagt taatagtgca
    22081 cttgcacttg aggtaatcgg caaatactct tagtgagtaa atgtttgccg tggtggcaag
    22141 gtgttagaac aacagagaat ataatgatgc aggatttgcg tctgatatta atcattgttg
    22201 gcgcgatcgc cataatcgct ttactggtac atggtttctg gaccagccgt aaagaacgat
    22261 cttctatgtt ccgcgatcgg ccattaaaac gaatgaagtc aaaacgtgac gacgattctt
    22321 atgacgagga tgtcgaagat gatgagggcg ttggtgaggt tcgtgttcac cgcgtgaatc
    22381 atgccccggc taacgctcag gagcatgagg ctgctcgtcc gtcgccgcaa caccagtacc
    22441 aaccgcctta tgcgtctgcg cagccgcgtc aaccggtcca gcagccgcct gaagcgcagg
    22501 taccgccgca acatgctccg cgtccagcgc agccggtgca gcagcctgcc tatcagccgc
    22561 agcctgaaca gccgttgcag cagccagttt cgccacaggt cgcgccagcg ccgcagcctg
    22621 tgcattcagc accgcaaccg gcacaacagg ctttccagcc tgcagaaccc gtagcggcac
    22681 cacagcctga gcctgtagcg gaaccggctc cagttatgga taaaccgaag cgcaaagaag
    22741 cggtgattat catgaacgtc gcggcgcatc acggtagcga gctaaacggt gaactgcttc
    22801 ttaacagcat tcaacaagcg ggcttcattt ttggcgatat gaatatttac catcgtcatc
    22861 ttagcccgga tggcagcggc ccggcgttat tcagcctggc gaatatggtg aaaccgggaa
    22921 cctttgatcc tgaaatgaag gatttcacta ctccgggtgt cactatcttt atgcaggtac
    22981 cgtcttacgg tgacgagctg cagaacttca agctgatgct gcaatctgcg cagcatattg
    23041 ccgatgaagt gggcggtgtc gtgcttgacg atcagcgccg tatgatgact ccgcagaaat
    23101 tgcgcgagta ccaggacatc atccgcgaag tcaaagacgc caacgcctga tacacttaag
    23161 gcaaattaac tcctcttcga acccccgctt gtcgggggtt tttagcattg atggtgcgat
    23221 atggaatcaa tcgaacaaca actgacagaa ctgcgaacga cgcttcgcca tcatgaatat
    23281 ctttatcatg tgatggatgc gccggaaatt cccgacgctg aatacgacag gctgatgcgc
    23341 gaactgcgcg agctggaaac caaacatcca gaactgatta cgcctgattc gcctacccaa
    23401 cgtgtaggcg ctgcgccgct ggcggctttc agccagatac gccatgaagt accaatgctg
    23461 tcactggata acgtttttga tgaagaaagc tttcttgctt tcaacaaacg tgtgcaggac
    23521 cgtctgaaaa acaacgagaa agtcacctgg tgctgtgagc tgaagctgga tggccttgcc
    23581 gtcagtattc tgtatgaaaa tggcgtttta gtcagtgccg cgacccgtgg cgatggcacc
    23641 accggggaag atatcacgtc taatgtgcgt actattcgcg ccattccgct gaagctgcac
    23701 ggagagaata tcccggcgcg tctggaagtg cgtggtgaag tgttcctgcc gcaggcgggg
    23761 ttcgaaaaga ttaacgaaga tgcgcgacgc acgggcggga aagtttttgc taacccacgt
    23821 aatgcggcag ctggttcact gcgtcagctt gatccgcgta ttacagcgaa gcgaccgctc
    23881 acttttttct gctatggcgt tggtgttctg gaaggtggcg agctgccgga tactcatctt
    23941 ggccgtttac tgcaatttaa aaagtggggg ttgccggtca gcgatcgggt aacgctttgt
    24001 gaatcggcgg aagaagtgct ggcgttctat cacaaagtgg aagaagaccg cccgacgctg
    24061 ggctttgata tcgacggcgt ggtgattaag gtcaactcac tgaaacagca ggagcagctt
    24121 ggctttgtcg cgcgtgcccc gcgctgggcg gtagcgttta aattcccggc gcaggagcag
    24181 atgacctttg tgcgtgacgt cgagtttcag gttgggcgta ctggcgcgat tacgcctgtt
    24241 gcgcgtctgg aacctgtcca tgttgctggc gtgctggtga gtaacgcaac cttacacaat
    24301 gcggatgaaa tcgaacgtct tggtttacgc attggcgata aagtggtgat tcgccgcgct
    24361 ggcgacgtga tcccgcaggt ggttgacgtc gtgctttctg aacgcccgga agatacccgt
    24421 gaggttgtat tcccgacgca ttgtccggta tgtggttctg acgttgagcg tgtggaaggt
    24481 gaagcggttg cccgctgtac cggtggcctg atttgcggtg cgcagcgtaa aaagtcgctg
    24541 aaacactttg tttcccgccg tgcgatggat gttgacggaa tgggcgacaa aatcatcgat
    24601 cagctggttg aaaaagaata tgtccatact ccggcagatc tgttcaaact caccgcaggc
    24661 aaactgaccg gactggagcg tatggggcca aaatcggcac aaaacgtggt taacgcgctg
    24721 gaaaaagcga aagaaaccac ctttgctcgc ttcctctatg cacttggcat ccgtgaagtc
    24781 ggcgaggcca ccgcagcagg tctggcggca tatttcggca cgctggaagc gctggaagcc
    24841 gcttcgattg aagagctgca aaaggtgcct gatgttggca ttgtcgttgc atcccacgtt
    24901 cacaacttct ttgccgaaga aagcaaccgc aatgtcatca gcgagctgtt ggcggaaggt
    24961 gttcactggc ctgcgccgat cgttatcaac gcggaagaga ttgacagccc gtttgctggt
    25021 aaaaccgtgg tgcttacggg cagcttaagc cagatgtcgc gtgatgacgc taaagctcga
    25081 ctggttgaac tgggcgcgaa agtcgcgggc agcgtgtcga agaaaaccga tctggtgatt
    25141 gccggagaag cggcaggttc taaactggcg aaagcgcagg aactgggcat tgaagtcatc
    25201 gacgaagcgg aaatgctgcg tttgctgggt agctgagatg gaaaaagagc agctgattga
    25261 aatagccaat acgataatgc cgtttggcaa atacaaaggg cgtcgcttaa tcgacctgcc
    25321 ggaggagtat ctgctgtggt ttgcccgcaa agatgagttc ccggcaggga agctcggtga
    25381 gctaatgcaa atcacgctgc tgattaaaac cgaggggctg acgcaactgg tccagcccct
    25441 gaagcgtccg ctttaagctt tatcggcgct gctttcctgc tgcgcctgta actgttcggt
    25501 ctggcgtttg tatcgacgcg ccagcaccgc acagaccatc aattggatct gatggaaaat
    25561 catcaggggc agcaccatca taccgatcac cgatgtgggg aacagaatgt ttgccatcgg
    25621 gatgccattt gccagactct ttttcgaacc acaaaagacg atagtaattt catctgcctt
    25681 attgaagccc agtcggcgtg ccataaagac gttaactaca atcacgatag ccagaagaac
    25741 gcagctgacc accacgataa acagcaatga tccccagcca actttatgcc agataccatt
    25801 aacgacggct tcgctgaacg ctgtataaac caccaacaga atggacgtct ggtcagtttt
    25861 cgcaatccat tttttattgc gcgacaccca gtcaccaatc cacggccggg aaagatgccc
    25921 caacacaaac ggcagcagca gttgcagcat aattttaccg acctgctcaa ggctgccccc
    25981 tgcaccgtga acattcatca ccagaccaac cagcaatggt gaaaggaaaa tccccagcag
    26041 gctggatgcc gacgcagaac aaaccgccgc cgcgacgtta ccgcccgcca ttgacgtgaa
    26101 ggcgattgca gactgcacgg tagccgggag aatgcacaag tagagaaaac cggagtagag
    26161 catcgggtcg acatttaccg gtttccacca ggcaaacagt acacccagaa tcggaaacag
    26221 cacgaaggtg ctgcacatta cccacaaatg cagtcgccag tgaccaccgc cagcaataat
    26281 cgcctcacgc gacaacttcg cgccgtgcat aaagaacagc agggcaatag ctgcggtggt
    26341 cagattttca aagaagggga cgaaatcgcc tctggccgga aagaaagagg ccagcaacac
    26401 caccgtgatc agggttaagg tgaaaggatc gaggatacga aaaagtttca taaagactcc
    26461 agatgatcga tggttctatt gtgcttcttc cagtttgaga aataaaattg atttattgca
    26521 tccattcatg aagaaaacag atgaattatt ctttaaaaca attaaaggtt ttcgtcacag
    26581 tagcgcagga gaaaagtttt agtcgtgcag gagagcgtat cggcttgagc cagtcggcag
    26641 taagtcacag tgtgaaggag ctggaaaatc atactggtgt tcgcctgctg gacagaacca
    26701 cgcgtgaagt ggtgcttaca gatgcagggc agcagttggc tttgcgtctt gagcgactgc
    26761 tggatgaact taacagcacg ttgcgcgata ccgggcgtat ggggcaacaa ctgagcggaa
    26821 aagttcgggt cgctgccagc cagaccattt ccgcgcatct tattccgcaa tgcattgctg
    26881 aaagccatcg ccgctatcca gatattcagt ttgtcctgca cgatcgtccg cagcagtggg
    26941 tgatggaaag tattcgccag ggagatgtcg actttggcat cgtcatcgat ccaggcccag
    27001 tgggcgatct gcaatgtgaa gcgattcttt ccgagccttt ctttctgctt tgccatcgcg
    27061 atagcgcttt ggccgtggaa gattatgtac cctggcaggc attacaggga gcgaaattgg
    27121 tgttgcagga ttacgcgtca ggcagccgac cgctgattga cgcagcgctg gcgcgcaatg
    27181 gtattcaggc gaatattgtg caggagattg gacatccggc gacgctgttc ccgatggtag
    27241 ccgcaggcat cggcatcagt attcttcccg cactggcgct accattgcct gaaggtagcc
    27301 cactggtggt aaagcgcatt acgccggttg ttgaacgcca gttgatgctg gtgcgcagga
    27361 agaaccgttc actttccacc gccgctgaag cgttatggga cgtggtacga gaccagggca
    27421 atgcgctaat ggctggtcgg gaaggggatc cgttgtatca gatatagata tcgatatgag
    27481 tcgttttttc aacaggattt agaagggata cattcagaga caaacactgc tctttctcct
    27541 gcgccttttc tgcctgctgt ttctgcaact gcgccagttg tgtttccagt agggtgattt
    27601 gctgttgaat caattcggcc tgtttttgct tgtcttcagc actgccgcta gtgtctacaa
    27661 tctctttaat ttgttgagtg agtttgatga tttgctgact tatccgcgcg atttgtgaag
    27721 caacatcgct accttgtgat gccctgttac tactctgtgt ttgtatggaa ggaattgaag
    27781 ttgaagagat agtggccatt gttcagtcct cgtcactgta cgaggtaata tcggtagaag
    27841 aacaaggaac ttgagaagaa aaatcaatct tacaaatcgc a
//
LOCUS       DAJSKJ010000032        27240 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000032 DAJSKJ010000000
VERSION     DAJSKJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.032"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            114..557
                     /gene="cmtB"
                     /locus_tag="ON345_001207"
     CDS             114..557
                     /gene="cmtB"
                     /locus_tag="ON345_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR3071300.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            585..1973
                     /gene="cmtA"
                     /locus_tag="ON345_001208"
                     /pseudo
     CDS             585..1973
                     /gene="cmtA"
                     /locus_tag="ON345_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
     gene            1988..3265
                     /locus_tag="ON345_001209"
     CDS             1988..3265
                     /locus_tag="ON345_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR3071301.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDSLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3262..3747
                     /gene="glpX"
                     /locus_tag="ON345_001210"
                     /pseudo
     CDS             3262..3747
                     /gene="glpX"
                     /locus_tag="ON345_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            complement(3712..3840)
                     /locus_tag="ON345_001211"
                     /pseudo
     CDS             complement(3712..3840)
                     /locus_tag="ON345_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
     gene            4212..5231
                     /gene="epd"
                     /locus_tag="ON345_001212"
     CDS             4212..5231
                     /gene="epd"
                     /locus_tag="ON345_001212"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR3071302.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            5281..6444
                     /gene="pgk"
                     /locus_tag="ON345_001213"
     CDS             5281..6444
                     /gene="pgk"
                     /locus_tag="ON345_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR3071303.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            6659..7738
                     /gene="fbaA"
                     /locus_tag="ON345_001214"
     CDS             6659..7738
                     /gene="fbaA"
                     /locus_tag="ON345_001214"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR3071304.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8096..8956
                     /gene="mscS"
                     /locus_tag="ON345_001215"
     CDS             8096..8956
                     /gene="mscS"
                     /locus_tag="ON345_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR3071305.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9095..9730
                     /gene="argO"
                     /locus_tag="ON345_001216"
     CDS             9095..9730
                     /gene="argO"
                     /locus_tag="ON345_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR3071306.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            9823..10563
                     /locus_tag="ON345_001217"
     CDS             9823..10563
                     /locus_tag="ON345_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR3071307.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            10730..11626
                     /locus_tag="ON345_001218"
     CDS             10730..11626
                     /locus_tag="ON345_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3071308.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(11623..13101)
                     /gene="scpC"
                     /locus_tag="ON345_001219"
                     /pseudo
     CDS             complement(11623..13101)
                     /gene="scpC"
                     /locus_tag="ON345_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(13125..13910)
                     /gene="scpB"
                     /locus_tag="ON345_001220"
     CDS             complement(13125..13910)
                     /gene="scpB"
                     /locus_tag="ON345_001220"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR3071309.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(13921..14916)
                     /gene="meaB"
                     /locus_tag="ON345_001221"
     CDS             complement(13921..14916)
                     /gene="meaB"
                     /locus_tag="ON345_001221"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR3071310.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(14909..17053)
                     /gene="scpA"
                     /locus_tag="ON345_001222"
     CDS             complement(14909..17053)
                     /gene="scpA"
                     /locus_tag="ON345_001222"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR3071311.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQIAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAITAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLEHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17257..18150)
                     /gene="argP"
                     /locus_tag="ON345_001223"
     CDS             complement(17257..18150)
                     /gene="argP"
                     /locus_tag="ON345_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR3071312.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18533..19192
                     /gene="rpiA"
                     /locus_tag="ON345_001224"
     CDS             18533..19192
                     /gene="rpiA"
                     /locus_tag="ON345_001224"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR3071313.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19448..20680
                     /gene="serA"
                     /locus_tag="ON345_001225"
     CDS             19448..20680
                     /gene="serA"
                     /locus_tag="ON345_001225"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR3071314.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            20909..20968
                     /gene="ibsC"
                     /locus_tag="ON345_001226"
     CDS             20909..20968
                     /gene="ibsC"
                     /locus_tag="ON345_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR3071315.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            21301..21360
                     /locus_tag="ON345_001227"
     CDS             21301..21360
                     /locus_tag="ON345_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR3071316.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(21461..22063)
                     /locus_tag="ON345_001228"
     CDS             complement(21461..22063)
                     /locus_tag="ON345_001228"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR3071317.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(22084..22267)
                     /gene="ssrS"
                     /locus_tag="ON345_001229"
     ncRNA           complement(22084..22267)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ON345_001229"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22309..22638)
                     /gene="zapA"
                     /locus_tag="ON345_001230"
     CDS             complement(22309..22638)
                     /gene="zapA"
                     /locus_tag="ON345_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR3071318.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            22806..23384
                     /gene="ygfB"
                     /locus_tag="ON345_001231"
     CDS             22806..23384
                     /gene="ygfB"
                     /locus_tag="ON345_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR3071319.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23410..24735
                     /gene="pepP"
                     /locus_tag="ON345_001232"
     CDS             23410..24735
                     /gene="pepP"
                     /locus_tag="ON345_001232"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR3071320.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYACADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24732..25910
                     /gene="ubiH"
                     /locus_tag="ON345_001233"
                     /gene_synonym="visB"
     CDS             24732..25910
                     /gene="ubiH"
                     /locus_tag="ON345_001233"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR3071321.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25933..27135
                     /gene="ubiI"
                     /locus_tag="ON345_001234"
                     /gene_synonym="visC"
     CDS             25933..27135
                     /gene="ubiI"
                     /locus_tag="ON345_001234"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR3071322.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         6513 a         6697 c         7259 g         6771 t
ORIGIN      
        1 taattcactg ttttcaaaac accggtttcc ctgctcaatt gctttcatta aaccgcacga
       61 gtattttatt tttaccgccg gacggtaccc taaaaataaa ataaggataa tttatgcggc
      121 ttagtgatta ttttccagaa tcatcaatct cagttataca ttcagcaaaa gattggcagg
      181 aagctatcga tttctcgatg gtatcattgc tggataaaaa ctatatcagc gagaattaca
      241 ttcaggcaat taaagattcc accattaaca atggccctta ttatattctc gcaccaggcg
      301 tggcaatgcc tcatgcgcga ccggaatgtg gggcgcttaa aaccgggatg tctttgacat
      361 tacttgaaca aggtgtttat tttccgggga atgacgaacc aattaaatta ctcatcggac
      421 tctctgctgc cgatgccgat tcgcacattg gcgctattca ggcgttaagt gaattactgt
      481 gcgaagaaga aatactcgaa caactcttaa cagcatcatc agaaaaacaa ttagcggaca
      541 ttatcagccg cggataatta ctctctcctc tacaggataa tattatggaa aacaagtctg
      601 ctcgtgcaaa ggtccaggct tttgggggct ttttgactgc aatggtcatc cccaatattg
      661 gtgcttttat tgcctggggt tttattactg cgttatttat tcctaccggt tggctgccta
      721 acgaacattt cgccaaaatt gtcggcccga tgattaccta tttattgccc gtgatgattg
      781 gttctacagg tggtcatctg gtcggcggta aacgcggggc ggtcatgggc ggaataggta
      841 ctattggtgt gatcgttggc gcagagatcc cgatgttcct tggctcaatg atcatggggc
      901 cgctcggtgg gttggtcata aaatatgtcg ataaggcact ggaaaaacgc atacctgccg
      961 gttttgagat ggttatcaat aacttctcat taggtatcgc ggggatgctc ctttgtctgc
     1021 tgggttttga agtgatcggc ccggcggtgt taattgccaa tactttcgtc aaagagtgta
     1081 tcgaggcgct ggtacatgcg ggttatctgc ctctgctgtc agtcatcaat gaaccggcga
     1141 aagtgctttt ccttaataat gcgatcgatc agggcgtcta ttatccgctg ggaatgcaac
     1201 aggcttcggt taacggtaaa tccatcttct ttatggtggc ctctaaccca ggtccgggcc
     1261 tggggctgct gctggcgttt accttgtttg gtaaagggat gagtaaacgt tctgcgcccg
     1321 gggcgatgat tattcacttc ctcggtggga tccacgaact gtatttccca tatgtgctga
     1381 tgaagccgct gactattatt gccatgattg cgggcggtat gtctggtacc tgaatgttta
     1441 acttactgga cggtggtctg gtggctggcc caagtccggg gtctatcttt gcttacctag
     1501 cactgacgcc gaaaggctcg ttcctggcga caattgccgg tgttacggta ggtaccctgg
     1561 tgtcctttgc tattacttcg ctgatactga agatggaaaa aacggtggaa acggagagcg
     1621 aagatgagtt tgctcagtca gccaatgcgg ttaaggcgat gaaacaagag ggtgcattct
     1681 cgttaagcag ggttaagcgt atcgcctttg tttgcgattc ggggatgggc tccagtgcga
     1741 tgggcgcgac caccttccgt aaacgcctgg aaaaagcggg gctggcaata gaagtaaaac
     1801 attacgcgat agaaaacgtg cctgcggatg ccgatatcgt cgtcactcac gccagtctgg
     1861 aagggcgcgt gaaacgtgtg acggataaac cgctgatatt gattaataac tatattggcg
     1921 atccaaaact cgacacttta tttaatcaat taaccgccga acataaacac tgattacaga
     1981 ggtaaaaatg aaaaccaaag ttgctgctat ttatggcaag cgggatgtcc gtctgcgcga
     2041 atttgaactg ccagaaatta ccgataatga actgttagtg agtgtaattt ctgacagcgt
     2101 ctgtttatcg acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
     2161 tttagaaaat catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg
     2221 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
     2281 gttaccaagc ggatattcag caggctacag ctacgaatat tttggcggta acgccaccta
     2341 tatgattatt cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
     2401 ttttgctgcg gcgtcgctgg cagagcctat gtgctgcatt attggtgctt atcatgccaa
     2461 ttatcacacc acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat
     2521 tgcactgctg gcgtgtgcgg gtccgatggg cattggcgct atcgattacg ccattaacgg
     2581 tggcatacaa ccgtcacggg tggtggtggt cgatatcgac gacaaacgcc tggcgcaggt
     2641 gcagaagctg ctgccggtgg atctggcggc cagtaaaggc attgagctgg tgtatgtgaa
     2701 taccaaaggg atgagcgatc cggtccagac gctgcgggcg ctgacaggag atgtcgggtt
     2761 cgatgacatt tttgtttatg cggcggtgcc tgctgtcgtt gagatggctg acgaattact
     2821 ggcggaagat ggctgtctga acttctttgc cgggccgacg gataaaaact tcaaagtgcc
     2881 gtttaatttc tacaacgtcc attacaacag cacgcacgtc gtcggtacat ccggcggttc
     2941 aacggacgac atgaaagagg caattgcgtt aagcgccacg gggcagttgc agccgtcgtt
     3001 tatggtgacc catatcggtg gtctggatgc ggtgccagaa accgtgctca atctgccgga
     3061 tatccctggc ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc
     3121 cgattttgcc gaaaaaggca aaaccgattc gctgtttaaa gagctggcgc ggctggttga
     3181 ggaaacgcac ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt
     3241 tgatatcggg gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa
     3301 caggcagcgt tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc
     3361 ctggcggtca ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt
     3421 atcggcgaag gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa
     3481 ggcgatgggc cagaagtcga tatcgcggtt gatcccattg aaggcacgcg gatggtggcg
     3541 atggggcaga gcaatgcgct ggcggtcatg gctttcgccc ctcgagatag cctgttacat
     3601 gcgtccgata tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat
     3661 ctgtctttgc ctctggcgga caacttgcgt aacgtggcgc gggcgttagg taatccaggc
     3721 ggcatgaagt tgatgctgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg
     3781 gaggaggcgc agtgcgtaaa agcgtgcgga ggaagaatat gggtagggca atgtcatcat
     3841 tggagtactc cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc
     3901 tgatataagt cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat
     3961 tctgcaaagt ggaataaatt ccacaggggc ggggctgaaa acgtgacgta agtcagataa
     4021 ttgctttctt tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa
     4081 gcgtttcagt cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa
     4141 ggtttccccg gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt
     4201 gcaggagatc tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg
     4261 tggttcgtgc tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg
     4321 aactggcgga tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt
     4381 ttgcatggga agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg
     4441 tattgcatga acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc
     4501 tcgactgcac cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg
     4561 ccaaaaaagt gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg
     4621 gcgtcaatca ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca
     4681 cgaattgcat aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca
     4741 ctgtgaccac aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg
     4801 acctgcgtcg cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg
     4861 ccggtatcac acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg
     4921 tgccaaccat aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag
     4981 ccaatgaagt caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg
     5041 actatacgga attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg
     5101 tcgatggcac ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg
     5161 ataacgaatg gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg
     5221 ctttcaggta agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc
     5281 atgtctgtaa ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg
     5341 gatctgaacg taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct
     5401 ctgccgacca tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg
     5461 ggtcgtccta ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac
     5521 ctgaaagaca aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac
     5581 gttgctgaag gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa
     5641 gacgacgaaa ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca
     5701 ttcggtactg ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt
     5761 gcgtgcgcag gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa
     5821 cctgctcgcc cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt
     5881 ctggactccc tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc
     5941 tttatcgcgg cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac
     6001 gaagctaaac gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca
     6061 accgagttct ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac
     6121 gagcagatcc tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat
     6181 gcgaaaacca ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa
     6241 ggtactgaaa tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc
     6301 ggcgacactc tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc
     6361 actggcggcg gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg
     6421 ctcgaagagc gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag
     6481 cgcgctttta gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac
     6541 aagagagtga atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg
     6601 cagcacatgc aacttgaagt atgatgagta taaggcccga cgatacagga caagagacat
     6661 gtctaagatt tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt
     6721 tttccaggta gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga
     6781 ctccatcaac gccgtactgg aaaccgctgc taaagttaaa gcgccggtta tcgttcagtt
     6841 ctccaacggt ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg
     6901 tgctgctatc ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta
     6961 tggtgttccg gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga
     7021 cggtctgttg gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc
     7081 tcacatgatc gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata
     7141 cctggagcgt atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg
     7201 tgaagaagac ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga
     7261 agacgttgat tacgcataca ccgaactgag caaaatcagc ccgcgtttca ccatcgctgc
     7321 gtccttcggt aacgtacacg gtgtttacaa gccgggtaac gtggttctaa ctccgaccat
     7381 cctgcgtgat tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgaa
     7441 cttcgtattc cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta
     7501 cggcgtagtg aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct
     7561 gaactactac aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga
     7621 agatcagccg aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc
     7681 gatgatcgct cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaaga
     7741 tattcctttc tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaaaa
     7801 ttgcctgatg cgctacgctt atcaggccta cacgtatcct gcaatatatt gaatttgcag
     7861 gtttttgtag gccggataag gcgttcacgc cgcatccggc atgacaaagc gcacattgat
     7921 atctgtctga gccccgctct gcggggattt tttcgccaaa agcaaggtga ttcagatgag
     7981 aaatctgtga tctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt
     8041 cataaggcgg cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga
     8101 agatttgaat gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc
     8161 gctgctgcta agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt
     8221 gattatcgcg cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga
     8281 tgccactgtt gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct
     8341 aatcgctgcc ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc
     8401 cgcaggctta gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt
     8461 gttacttgtc atgttccgcc cgttccgtgc cggagaatat gttgacctcg gcggcgtagc
     8521 cggtactgtg ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat
     8581 tatcgttatt ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc
     8641 agttcgccgt aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa
     8701 gcagatcctg accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac
     8761 tgtgcgcctg aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa
     8821 cagcggcgat ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga
     8881 tgccgccggt atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga
     8941 agacaaagct gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta
     9001 gttattctga ttgctaatta atattatcaa tttccgctaa taacaatccc gcgatatagt
     9061 ctctgcatca gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct
     9121 tgctcttggg gcagctatga ttctgccgct cggcccacaa aatgcttttg tgatgaatca
     9181 gggcattcgt cgtcagtacc acattatgat tgccttactt tgcgctatca gcgatttggt
     9241 cctgatttgc gcggggattt ttggtggtag cgcgttattg atgcagtcgc cgtggttgct
     9301 ggcgctggtc acctggggcg gcgtagtctt cttgctgtgg tatggttttg gcgcttttaa
     9361 aacagcgatg agtagtaata ttgagttagc tagtgccgaa gtcctgaagc aaggcagatg
     9421 gaaaattatc gccaccatgt tggcagtgac ctggctgaat ccgcatgttt acctggatac
     9481 ttttgttgta ctgggcagcc ttggcgggca gcttgacgtc gaaccaaaac gttggtttgc
     9541 gctcgggaca attagcgcct ctttcctgtg gttctttggt ctggctattc tcgcagcctg
     9601 gctggcaccg cgcctgcgca cggcaaaatc acagcgcatt atcaatctgg tagtgggatg
     9661 tgtcatgtgg tttatagcct tgcagctggc gagagacggt attgctcatg cacaagcctt
     9721 gttcagttag gcgctatctg atggaaaaat aaaacagagg cgctaagctt gcctccagag
     9781 gtcctgaatt ttgggcaagt caattaacac ggagagacta acgtgaagtt caaagttatc
     9841 gccctggcgg cattaatggg tattagcggg atggcagcgc aggctaacga attgccggat
     9901 ggaccgcata ttgtcacctc cggtacggca agtgtggatg cggtgccaga cattgccact
     9961 cttgcgattg aagttaacgt ggccgcgaag gatgccgcta cagccaagaa acaggcagat
    10021 gagcgcgtcg cacaatacat ttccttcctt gaactcaatc agatcgcgaa aaaagatatc
    10081 agctcagcga atttacgcac ccagccagat tatgattatc aggatggtaa aagtatcctg
    10141 aaaggctacc gcgctgtgag aacggtggaa gtcacgctcc gtcagttaga caaactgaat
    10201 tccttgctgg atggcgcgct gaaggcgggt cttaacgaaa ttcgttctgt gtcgctgggc
    10261 gtggcgcagc cggatgccta taaagacaaa gcgcgtaagg cagcgattga taacgcgatt
    10321 catcaggcgc aggaactggc gaacggcttt catcgtaaac tggggccggt atatagcgtg
    10381 cgctaccatg tttccaacta tcagcccagc ccaatggtgc ggatgatgaa agccgatgcc
    10441 gcgccggtgt ctgcccagga aacttacgag caggccgcta ttcagtttga tgatcaggtc
    10501 gatgtggtct tccagttaga acctgtggat caacaacccg ctaaaacacc tgccgcacaa
    10561 taatacttgt agcccgacgt attcgtcggg ctaatgcctt tacccgatat tgcctccttt
    10621 ttctgtaatt ttctgtaaaa ccatgacgta atcacctatc tttgctcgcg gtttcatcaa
    10681 ggataggctg actgaaacgg ttaagcggac aaggatgcgg gggaggggga tggacatttt
    10741 tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata ttgcccgggc
    10801 ggcagaaaaa atccatatga cggcttcgcc atttggcaaa agcattgccg cgctggaaga
    10861 acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca ataaagccgg
    10921 tcaggaactg tatcaaaaac tgttcccggt ttatcaacgg ctttctgcga ttgataatga
    10981 aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata atacttatcc
    11041 gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg tgaccgccca
    11101 gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc agttagattt
    11161 tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc tgactatcag
    11221 tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag agcgtcaaga
    11281 gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc gggctaactt
    11341 cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca ttatttatcg
    11401 tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc tgacggtgat
    11461 cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg atgcgccgca
    11521 caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt ataccgccac
    11581 gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca gcatcgagcc
    11641 ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt gaatatgtcc
    11701 gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat gtgcacaatt
    11761 atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg cgatcccttg
    11821 ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc acattgacac
    11881 gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg acagatacgc
    11941 gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc cggctacgtg
    12001 cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag cgatgacgcc
    12061 cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca caatgcggct
    12121 ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga ttgtcaggct
    12181 ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccaccg attcctgtag
    12241 cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac gcgccattac
    12301 cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg gaatacgccc
    12361 atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct ggcacatggg
    12421 attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga cggcgacaat
    12481 ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat ggaagatcga
    12541 cacgctattg cgccgcggtg gcgcgccagg aatcacaata tctgccagtt ctgcaacgcg
    12601 cggatcgtga tagtgattaa gttcaatgat cactttcttc gctcgcagca gctaggtcgg
    12661 cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg ccgatgcttc
    12721 aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg ccacttcgct
    12781 caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta aaccggacga
    12841 tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat cgtcagcggc
    12901 ggcgctgatt gacgcccccg tcagcaggcg aatttgatac ggctttttgg cctcatgctg
    12961 ttcgttagct ctgcgggcaa tcgcggtggg tagggctttc ggcgaacccg ccggggtaaa
    13021 gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat cggcggtcat
    13081 ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg accaacgaaa
    13141 ttaggtttac gtttttcgag gaaagcgttc attccttcct ggtaatcttc gctgtcataa
    13201 accgcgcgtc gcatcccctg aatacgttca aattcatcgg agttcatggt gtgcgcttcg
    13261 cccagtacac gcagctcttc tttgataaca gcaatggcta acggcgcttt ctcagagatg
    13321 tggtgcgcca tttgtaaggt gaaatcttcc agttcttcca cttccacaac atggttgagg
    13381 atgccgacag ccagcgcgcg ctgggcggtg attggcgaag cggtaaaaat cagctctttg
    13441 acaatgtgga agcccgcgtc gcgggtcagg ttgtgaatgc cgaccaggtt atacgggacg
    13501 ccgaggttta caggcgtcat tgagaaggtt gaggtactgg cggcgatgat cagatcggaa
    13561 ctcatgatca tttcaaatgc gccaccccaa acactacctt ccaccatcga aatgatcggt
    13621 ttcgggaatt tttggatcat gcgggtaatt tgacgcaatg gatcatcata ggagagcgga
    13681 tcgcgaccgc cagacggcag ttcgtgaata tcgtgacctg cggagaagac tttggatcca
    13741 ctcggcgcgc gcaaaatgat acagcgaatt tccggccggt tgagatcgct taacgcctgc
    13801 ataagatcat caataaagac tttacttaag gcattaagtt ttcggccata gttaaactca
    13861 atgaccgcca ctttgttgat agtggcaacg ttcacatact gataagacat aaaaattcct
    13921 ttaatcaaaa tattgcgtct ggataaattc actgagctgc cgcaggccgg tgcgcggtga
    13981 gagcgtattg tttttgaccg ctaaaagcgt ctggcggtaa tagcgatcga aatcttcatt
    14041 cgcgaacagg tgattcagta cttcttcttc ggtctgctta cgcagccatt ccaccgattg
    14101 ttgttgccgc acttgttgta aacgaccact ggcagttagc gcggttttga actcgatgat
    14161 ggcgtgccag atctcatcga ttccacgttt ttccagtgcg ctacaagtca gaacccgtgg
    14221 ctgccattcg tcgtatttac gtcgcagaat atgcagggca ctctcgtaca tatgccgggc
    14281 aatggcgaca ttggtatggt tatcgccatc gtctttgttg ataacgatca gatcagccac
    14341 ttccatcagc ccttttttaa tgccctgcag atcatcgccg ccaccggcaa tttgcaacga
    14401 gataaaacag tccaccatgc gggcgacttc tgtttccgac tgcccgacgc caaccgtttc
    14461 gacaatcact acgtcataac ccgccgcttc gcataacagc attaattccc gcgctcgctg
    14521 actggcaccg cccagatgac cggaggatgg taccgggcga ataaacgccg tctcggcacg
    14581 cgccaggtca ttcatgcggg ttttatcccc gagaatgcta ccgccagtga ccggactgct
    14641 gggatcgacc gcaataacag caacctttaa tccctcacga atcaacaaca tgccaaaggc
    14701 ttcaagaaag gtacttttcc ccgcgccggg tgtgccggta acgcccagtc gcagggcgtt
    14761 accgcagtac ggcataatgg catcaagcag ttgcgtactt agcgcctgat gacgcgggtg
    14821 acggctttcc accagcgtca tggcctgggc gagtgtggca cgctcaccct gacgtaagcg
    14881 gcgaatactt tctgccagcg tggcttcatt aatcatgatg ctggcttatc agattcagta
    14941 cgtcgcgcac actgtcgagc ataggtgtac ctggaccata aatcgccgcc acgccgcgct
    15001 cttgcaggaa ggcgtaatcc tgcggtggaa tgacgccacc ggcgactacg cagatatctt
    15061 cgcgtcccca ttttttcagc gcttcgacca gttccgggat cagcgtttta tgaccggcag
    15121 ccagtgagga tgcgcccact acgtgaacgt cattttctac tgccaggcgg gcgatctctt
    15181 caggtgtaga gaacatcggg cttaaatcta cgtcgaaacc gagatcggaa taggcgctgg
    15241 cgatcacttt cgcgccgcga tcgtgtccat cctggcccat tttggcgatc agaatgcgcg
    15301 ggcgacgacc attgtcggca aggaactgct ccgtttgcgc aacaatggca tcgaactcgg
    15361 aggccgattt ctcagattga tgatagcttt gcgcaatcac gccggtaaca cactggcttg
    15421 gcaccagata acggtcgaaa gcggcttcca gcgcatcgga aatttcaccc aaggtggcgc
    15481 gaacgcgagc ggcattaaca gcggcagcca gcaggttttc gttatgctgt gcggcgtgag
    15541 tcagggcgtt caacgcggcg gttacggcgg catcatcacg ggtggcgcga atgcgttcca
    15601 gcgaagcaat ttgctcgtta cgcaccatca cgttgtcgat ctcaagtaca tcggtttcgt
    15661 cttcgtgctc cagtttgtac ttgttgacac caacgatgac acgcttgccc tggtcgatca
    15721 gcgactgttc gcgcgctgag gcctcttcga tcattcgttt tggcagacct gcttcgatcg
    15781 ctttcgccat gccaccggct tcgtcgatct gttggataat agctctggct tgtttgacga
    15841 tttgatcggt cagcgactca atgtaatagg atccggccag tggatcgacg gtgcggcaga
    15901 gttctgattc ttcctggatg atgatctggg tgttgcgggc aatgcgtgct gagaaatcgg
    15961 taggcaaacc aagcgcttcg tcaaaggcgt tggtatgcag tgactgagta ccgcccagcg
    16021 tcgcagccag cgcttcaatg gtggtgcgga taacgttgtt atacggatcc tgttcagtca
    16081 ggctccagcc tgaggtctgg cagtgggtac gcagcgccag tgatttcggg tcctgtgcgc
    16141 caaatccact gaccgcttcg ctccataaat aacgtgccgc acgcaacatg gcgacgttca
    16201 taaacagatc catgccgatg ccgaagaaga acgacaggcg aggagcgaag tcatcaattt
    16261 tcagtccggc agtgattgct gctttgatgt actcaatccc atcagcgagc gtaaatgcta
    16321 cctgctgcac gcagttggca cccgcttcac ccatgtggta accgctgata ctgatggtat
    16381 taaatcgcgg catgttgccg gaacaccagg cgatgatgtc ggcgataatg cgcattgacg
    16441 gttttggcgg gtaaatatag gtgttgcggc agaggtactc tttgaggata tcgttctgaa
    16501 tggtgccggt cagtttatca ggtgtaacac cttgctcttc cgcggcgacg atataaaacg
    16561 ccagtactgg tagcactgcg ccattcatgg tcatcgaaac cgacatttta tccagcggga
    16621 tctggtcgaa caggactttc atatcttcca cggtgtcgat agcgacgccc gctttgccga
    16681 cgtcgcccgc cacgcgcggg ttatcggagt cgtagcctcg gtgggtggca aggtcaaacg
    16741 caacggaaag acctttttgc ccggcggcca ggttacggcg ataaaaagcg ttggactctt
    16801 ttgctgttga aaaaccagca tactgacgga tggtccacgg ttgggcggta tacatagtgg
    16861 cacgcgggcc acgaacgtag ggcggcaaac caggaagggt acctgtcacc tccagattat
    16921 cgagatcggc ttcggtatac agcggcttga tggcgatccc ttccgcgatt tgctgaacca
    16981 gcgagtcgac agttttctcc cgacggctca attccttgtt ggcaagctgt tgccactcct
    17041 gcacgttaga cattatttgc tccgtaaaac gcaatcaaaa tgagggtatt tgcgcctacg
    17101 gtgagctttt tacgtaggtt tgtcgccagc aaaaaaaatg cattcagacg tcgttttttg
    17161 ccgcctgaga tttgtctatt ttgtgatgtg attcaaggaa tacaaaaaga agtgaggaca
    17221 acgcctgata tgtgctatca ggcattattt gatggattaa tcctgacgaa ggactttgtg
    17281 accataatcg agtaacgcat cagtgacttt acgcatcatg cggctttcag gagcaaagcg
    17341 gtgccagtag agcatccgtc gttgaaatag cccaggcgtt aagtcaatca gttcaccgct
    17401 ggccagctct ttctcgattt gcaggtgcgg gatcatacag caggtggtgc cctggcgagc
    17461 aagttgtacg aacgcttctg aagaattaac gatatggcag ggcacgctgc ctggaggcag
    17521 atcgaagttt tgctgcaaaa aggcctggtg catatcgtca agatggtcaa aggcgaccac
    17581 aggtgctttc aataatgccg aacgcgttac gccgttaggg aaatattttt cggcaaacgg
    17641 ttttgagctg acgaacagat agtcaagcgc accgagctta tcgacaaggc aactcggcag
    17701 cgcctgatgt tgaatactca ccgcgccgac cacttcgccg cggcgcagac gttcctgagt
    17761 acgggtttca tcttctacct gcaagttgag gcggataggc gaatcagcca acacaggagc
    17821 cagtgccgga agcaaccacg tcgccagact gtcggcgttg accgccagtg aaagcagcag
    17881 cggagtcgaa ccggtttgtt catcgcccag ccactcttct tccagcaact ccacctggcg
    17941 cagcagtgcc agcagttttt gcccttgttc cgtcgggcgc ggcggtacgg tacgcaccaa
    18001 cagcggctgc ccgaacatat tttccagttg cttaatgcgc tgtgagacgg ctgattgtgt
    18061 aatgcacagc ttttgtgccg cgcgctcaaa tcctcgttca cgtatcaccg catccagtgc
    18121 ctgtaatgtt ctgtagtccg ggcgtttcat tgctgttgtc tgctcctggt aaatgctttt
    18181 tcctgcacta tgacacaatt ttgtgtcagg ttgcatatac cgcagccgtc ggcgggtaac
    18241 atttgtatca atggttaaac gcgcagctta aaagatcagg gatttgcgct attgcatttc
    18301 gcttagcgac ttcgcgccct ggttatgttg aatggcgtgg cgttattgcc tctgttcgca
    18361 ccgtggagcg gcatacccag accgttgtat gctttatttg cggggcgaaa agggatgccc
    18421 gccattgcgc gatattttat ggatgagtta accacgcggc ttgccaacgg ggtctgaatc
    18481 gctttttttg tatataatgc gtgtgaaatt tcataccaca ggcgaaacga tcatgacgca
    18541 ggatgaattg aaaaaagcag taggatgggc ggcacttcag tatgttcagc ccggcaccat
    18601 tgttggtgta ggtacaggtt ccaccgccgc acactttatt gacgcgctcg gtacaatgaa
    18661 aggccagatt gaaggggccg tttccagttc agatgcttcc actgaaaaac tgaaaagcct
    18721 cggcattcac gtttttgatc tcaacgaagt cgacagcctt ggcatctacg ttgatggcgc
    18781 agatgaaatc aacggccaca tgcaaatgat caaaggcggc ggcgcggcgc tgacccgtga
    18841 aaaaatcatt gcttcggttg cagaaaaatt tatctgtatt gcagacgctt ccaagcaggt
    18901 tgatattctg ggtaaattcc cgctgccagt agaagttatc ccgatggcac gtagtgcagt
    18961 ggcgcgtcag ctggtgaaac tgggcggtcg tccggaatac cgtcagggcg tggtgaccga
    19021 taatggcaac gtgatcctcg acgtccacgg catggaaatc cttgacccga tagcgatgga
    19081 aaacgccata aatgcgattc ctggcgtggt gactgttggc ttgtttgcta accgtggcgc
    19141 ggacgttgcg ctgattggca cacctgacgg tgtcaaaacc attgtgaaat gatctaacgg
    19201 gggaacctcc cccgttaaaa aaattctctt cattaaattt ggtgacatgt gtcacgtttt
    19261 tacctagcta ttgtcgattg ctctaaataa atcctctaaa ccagcatatt caaccaagaa
    19321 atacctttgc gtgatatttc ctcaacatcg cgacgcaaac gttcatattg ccgcaatatt
    19381 attttttgat atgttgaaag gcggatgcaa atccgcacac aacatttcaa aagacaggat
    19441 tgggtaaatg gcaaaggtat cgctggagaa agacaagatt aagtttctgc tggtagaagg
    19501 cgtgcaccaa aaggcgctgg aaagccttcg tgcagctggt tacaccaaca tcgaatttca
    19561 caaaggcgcg ctggatgatg aacaattaaa agaatccatc cgcgatgccc acttcatcgg
    19621 cctgcgatcc cgtacccatc tgactgaaga cgtgatcaac gccgcagaaa agctggtcgc
    19681 tattggctgt ttctgtatag gaacaaacca ggttgatctg gatgcggcgg caaagcgcgg
    19741 gatcccggta tttaacgcac cgttctcaaa tacgcgctct gttgcggagc tggtgattgg
    19801 cgaactgctg ctgctattgc gcggcgtgcc ggaagccaat gctaaagcgc accgtggcgt
    19861 gtggaacaaa ctggcggcgg gttcttttga agcgcgcggc aaaaagctgg gtatcatcgg
    19921 ctacggtcat attggtacgc aattgggcat tctggctgaa tcgctgggaa tgtatgttta
    19981 cttttatgat attgaaaata aactgccgct gggcaacgcc actcaggtac agcatctttc
    20041 tgacctgctg aatatgagcg atgtggtgag tctgcatgta ccagagaatc cgtccaccaa
    20101 aaatatgatg ggcgcgaaag aaatttcact aatgaagccc ggctcgctgc tgattaatgc
    20161 ttcgcgcggt actgtggtgg atattccggc gctgtgtgat gcgctggcga gcaaacatct
    20221 ggcgggggcg gcaatcgacg tattcccgac ggaaccggcg accaatagcg atccatttac
    20281 ctctccactg tgtgaattcg acaacgtact tctgacgcca cacattggcg gttcgactca
    20341 ggaagcgcag gagaatatcg gcctggaagt tgcgggtaaa ttgatcaagt attctgacaa
    20401 tggctcaacg ctctctgcgg tgaacttccc ggaagtctcg ctgccactgc atggtggtcg
    20461 tcgtctgatg cacatccacg aaaaccgtcc gggcgtgcta actgcgctga acaaaatctt
    20521 cgccgagcag ggcgtcaaca tcgccgcgca atatctacaa acttccgccc agatgggtta
    20581 tgtagttatt gatattgaag ctgacgaaga cgttgccgaa aaagcgctgc aggcaatgaa
    20641 agctattccg ggtaccattc gcgcccgtct gctgtactaa ttccccttct ctgaaaatta
    20701 acgggcaggt cactgacttg cccgtttttt tatcccttct ccacaccgtt gccgcgttgc
    20761 gcattctact tttcgagtct gctccagaaa ataaatcaaa atcagatcaa tgcgttgtgt
    20821 ttatttaatg gggatttacg atggcgggac agcatggggc tgtaacagta aagccctcac
    20881 cgaagcgagg gcttaaagga gaagggttat gatgcgactt gtcatcatac tgattgtact
    20941 gttactgata agtttcggag cttattaaca gtcagtctca ggggaggagt tatcctccct
    21001 tacccttact cactaaatta ggtcaaagaa tcaacgatgt caatcagggc gatgcggggt
    21061 gcatcgccct gaaccgaact ttttgatagc cacttcatcg cccacgttta ctgccgtaat
    21121 agatcctttt tcccaaaaat tatctctgtt ttgcatgggg tttttcgagc gcgctcgaag
    21181 aaaagattta aaggtagatc aatgcgttgt ggttatttaa agagagctta cgatagctgg
    21241 gcagcatggg gctgtaacgg taaagccctc accgaagcga gggcttgaag gagaagggtt
    21301 atgatgcgac ttgccatcat actgattgta ctgttactca taagtttccc agcttattaa
    21361 cagtcagtca caggggagga gcaatcctcc cttaccctta ctcactaaat taggttaaag
    21421 aatcaacgat gtcaatcagg gcgatgcagg tgtatcgccc ttaccactcc cagactttcg
    21481 acggtgtaac caccgcagga agagggatat cccactcttc aacggggagt ttttccacca
    21541 actgacaatc atgcgcataa cccaccggtt gcgttttata gtgctgccag ttttgtaagg
    21601 tccgatcata aaaaccaccg cccattccca ggcgctgacc gtactcatca aaggcgacca
    21661 gcggtgtgat cagcacgtct aatcgggaaa ggggtagcac gtcacgcaca tccaattttg
    21721 gctcatggat cttcaacctg ttcatcacca gttcgctttg cggatggtaa ttcaggaaca
    21781 gcaaattacc ggcactaaag ggatgcaaaa ctggaagata tacgcgctta ccggcgcgcc
    21841 agagttgttc tatgagtggc tgggtgtcga gttcgccatc aaaagagagg aatacagcga
    21901 ccgtatgtgc catcaccacc gggggataag tcatcatccg ggtagcggct tgttgaccca
    21961 tttcctgctg ttgttccggc gttaacgcac gacgacgttg ccgaatcatt ttgcggattt
    22021 cttgtcggga taatgtcagt gggagttctg gtagttgcgt catggctggt gccagataag
    22081 aagggaatct ccgagatgcc gccgcaggct gtaacccttg aacccttggt tcaaggtgaa
    22141 tgtgtcgtcg cagttttaag gcttctcgga cggaccgagc atgctcacca accgcggagc
    22201 gccacattct tgtggtatga aatatcggct caggggactg gcccgcttgc gaacatctca
    22261 gagaaatttt gtcttcacgg ttactctacc acagtaaacc gaaaagtgtc attcaaagtt
    22321 ttggttagtt ttttcggtga tgcgaccttg ttcaagtaac gcttgttcta tggtctgctg
    22381 cagcatccga atacgctgtt ccatacttgc cgcgtagtca cgagtctttg ctttttcttg
    22441 cgctaactca tagctgatat tcaatgcggc aatgaagacc aactgttcag tatttgtgac
    22501 tctagtgcgt tctttcagat cttgcaaccg ttggttcaga tcgtccgctg cctgattcaa
    22561 cgcatccctt tggtcaggcg ggcagttcac acgcagtgaa cggccaaaaa tttggatatc
    22621 gacgggttgt gcagacatgc caccttcctg ctgattgact gcgctgcctt cgtctttaga
    22681 cccatggtct gcgaaggggc gacactatag ctaccctgat gagaagagac aagccctttt
    22741 ctggtccacc agggcccaaa gtggtagcat atcatgaata ttcctccctt tgacgacgaa
    22801 tgcttatgtc tatacagaac gaaatgcctg gttacaacga aatgaaccag tatctgaacc
    22861 aacaagggac gggtctgacc cctgctgaga tgcatggttt aatcagtggg atgatatgtg
    22921 gcggtaacga tgacagctca tggctaccgc tacttcacga cctgacgaac gaaggcatgg
    22981 ctttcggtca tgagctggca caggcactgc gtaaaatgca ctctgccacc agcgatgccc
    23041 tgcaggatga cggcttcctt tttcagcttt atctgcctga tggcgatgat gtcagcgttt
    23101 tcgatcgggc tgatgcattg gcaggttggg tcaatcactt cctgcttggt cttggcgtta
    23161 cgcaaccgaa gctggataaa gtgaccggcg aaaccggtga agctatcgac gatctgcgta
    23221 acattgcgca actgggttac gacgaagacg aagatcagga agagcttgaa atgtcgcttg
    23281 aagagatcat cgaatacgtt cgtgttgccg cgctgttatg ccacgacacc tttactcatc
    23341 cgcaaccgac cgcgccagaa gtacaaaaac cgactctaca ctaaaaacaa aaaacgtaag
    23401 gagagtgtta tgagtgagat atcccggcaa gagtttcagc gtcgccgtca ggccctggtg
    23461 gagcaaatgc aacccggcag cgccgcgctg atttttgctg caccagaagt aacacgtagc
    23521 gccgacagcg aataccccta tcgtcagaac agtgacttct ggtacttcac cggctttaac
    23581 gaaccggaag cggtgctggt gctgattaaa agcgatgaca ctcataacca cagcgttctg
    23641 tttaaccgcg ttcgcgacct gacggcggag atctggtttg gccgtcgctt aggccaggat
    23701 gccgcgccag agaaactggg cgttgaccgc gcactggcat tcagcgaaat caatcagcaa
    23761 ctttatcaac tacttaacgg cctggatgtg gtttaccatg cccagggcga atatgcatgt
    23821 gctgatgaaa tcgtgaacag tgcgctggaa aaactgcgta aaggttcgcg gcaaaatctc
    23881 accgcaccgg caacgatgat cgactggcgt cctgttgttc atgaaatgcg cctgttcaaa
    23941 tcgccagaag agattgccgt actccgccgc gcgggagaaa tcaccgccat ggcacataca
    24001 cgggcgatgg aaaaatgccg tccgggaatg ttcgagtacc atctggaagg cgaaattcac
    24061 cacgaattta accgccacgg tgcgcgctat ccgtcctata acaccattgt cggcagcggt
    24121 gaaaacggct gcattctgca ctacaccgaa aacgagagtg aactgcgcga cggtgacctg
    24181 gtgttgattg acgcgggttg tgaatacaaa ggttacgcgg gcgatattac ccgcaccttc
    24241 ccggtcaacg gcaaattcac ccaggcccag cgtgaaatct acgacattgt gctggagtct
    24301 ctcgaaacca gcctgcgcct gtatcgtccg ggaacttcca ttctggaagt cactggtgaa
    24361 gtggtgcgca tcatggttag cggcctggta aaactcggca tcctgaaagg tgatgttgat
    24421 gaactgatcg ctcagaacgc ccatcgtcct ttctttatgc atggccttag ccactggtta
    24481 ggactggatg tccatgacgt tggcgtttat ggtcaggatc gctcgcgcat tctggaaccg
    24541 ggcatggtac tgaccgtaga gcctggtctg tatatcgcgc cggatgcgga tgtgccagaa
    24601 caatatcgcg gtatcggcat tcgtattgaa gacgacattg tgattaccga aaccggcaac
    24661 gaaaacctca ccgccagcgt ggtgaaaaag ccggaagaaa tcgaagcgtt gatggctgct
    24721 gcgagaaagc aatgagcgta atcatcgtcg gtggcggcat ggcgggcgcg acgctggcgc
    24781 tggctatttc ccggttaagt cacggggcgc tgccggtaca tttgattgaa gcgactgcgc
    24841 cagagtcaca tgctcatccg ggctttgatg gacgagcgat agcgctggcg gcgggtacct
    24901 gtcagcaact ggcgcgcatc ggcgtctggc aatctctggc tgattgcgca accgccatca
    24961 ctaccgtgca tgtcagcgat cgtggtcatg ctggattcgt caccctcgcc gcagaagatt
    25021 accaactggc ggcgctgggg caggttgtcg aattgcataa tgtcgggcaa cggctgtttg
    25081 cattgctgcg aaaagcacct ggcgtaatgc tgcattgccc cgatcgcgta gctaacgttg
    25141 cccgtactca gagtcacgtt gaagtgacgc tggagagcgg cgagatgctg acgggccgcg
    25201 tgctgatagc cgctgatggc acccattcag cgttagccac cgcgtgcggc gttgattggc
    25261 agcaggagcc ttacgaacaa ctggccgtga ttgccaacgt tgcgacttcc gttgcgcacg
    25321 aaggccgcgc ttttgaacgc ttcacgcaac atggcccgct ggcgatgttg ccgatgtctg
    25381 acggacgctg ttcgctggtc tggtgtcatc cactggaacg gcgcgaagag gtgctgtcgt
    25441 ggagcgacga gaagttttgc cgtgaactcc agtcggcctt tggctggcga cttggaaaaa
    25501 ttacccacgc cggtaaacgc agtgcttatc cgctggcgtt aacccgcgcc gccagagcta
    25561 ttacccatcg taccgtgctg gtgggcaatg cggcgcaaac tctgcacccg attgccgggc
    25621 aagggtttaa cctcggtatg cgagatgtca tgagccttgc ggaaaccctg actcaggcgc
    25681 atgagcgcgg agaagacatc ggtgattacg gcatattgtg ccgctatcag cagcgtcgac
    25741 agagcgatcg cgaagcaacc atcggcgtca cggacagcct ggtacatctt tttgccaacc
    25801 gttgggcacc gctggttgtc gggcgcaaca tcggactgat gacgatggaa ttattcaccc
    25861 cggcacgcga tgtgctggcg cagcgcaccc tcggttgggt ggcgcgttga aggcggtttt
    25921 aaggaataaa caatgcaaag tgttgatgta gccattgttg gtggcggcat ggtggggctg
    25981 gcggttgcct gcggcttaca ggggagcggc ttacgcgttg ccgtactgga gcagcgcgta
    26041 ccggaacctc tggcggcgga tgcaccacca caactgcgcg tttcggctat caatgccgcc
    26101 agcgaaaaat tactcacccg tcttggcgtc tggcaggaca ttctctctcg tagggccagt
    26161 tgttatcacg gtatggaagt gtgggacaaa gacagttttg gtcacatttc gtttgacgat
    26221 caaagcatgg gctatagcca tcttgggcat atcgttgaaa actcagtgat tcactacgcg
    26281 ctgtggaaca aagcgcagca gtcgtcagat atcactctgt tggcccccgc agaattacag
    26341 caggtcgcct ggggagaaaa tgaaactttc ctgacgctga aagatggcag catgttaacg
    26401 gcgcgtctgg tgattggcgc ggacggtgct aattcctggt tgcgcaacaa agcagatatt
    26461 ccgctgactt tctgggatta tcagcatcac gcgctggtag cgaccattcg cacggaagaa
    26521 ccgcatgatg cggtggcgcg gcaggttttc catggcgaag gcattctggc ctttttaccg
    26581 cttagcgatc cgcatctttg ctcaattgtc tggtcactgt cgccagagga agcgcagcgg
    26641 atgcagcagg caagtgaaga tgaatttaat cgcgcgttaa atatcgcttt tgataatcgc
    26701 ctgggcttat gcaaggttga gagcgcgcgt caggtgttcc cactgacggg gcgttatgcg
    26761 cgccagtttg ccgcgcaccg tctggtgctg gtgggcgacg ccgcgcatac cattcacccg
    26821 ctagcggggc agggagtaaa tctcggcttt atggatgctg cagagctgat tgctgaactg
    26881 aaacggttgc atcgtcaggg gaaagacatc gggcagtaca tttacctgcg ccggtatgag
    26941 cgtagccgca agcacagtgc ggcgctgatg ctggctggta tgcagggatt ccgcgatctg
    27001 ttttccggta ccaatccggc gaaaaaactg ctgcgtgata ttggtttgaa actggccgac
    27061 acgcttcctg gcgttaaacc acaacttatc cgccaggcaa tgggattaaa cgatttgcct
    27121 gaatggctgc gctaaaaatt tctcttctgc tgtttatttg atacccatca cactttcatc
    27181 tcccggtttt ttcgccggga gattttcctc atttgaaata aactaatttc acctccgttt
//
LOCUS       DAJSKJ010000033        26851 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000033 DAJSKJ010000000
VERSION     DAJSKJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.033"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            11..757
                     /gene="ycaL"
                     /locus_tag="ON345_001235"
     CDS             11..757
                     /gene="ycaL"
                     /locus_tag="ON345_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR3071323.1"
                     /translation="MLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAK
                     AIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAM
                     ANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSG
                     VASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASL
                     DGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            930..1613
                     /gene="cmk"
                     /locus_tag="ON345_001236"
     CDS             930..1613
                     /gene="cmk"
                     /locus_tag="ON345_001236"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR3071324.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIIERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1724..3397
                     /gene="rpsA"
                     /locus_tag="ON345_001237"
     CDS             1724..3397
                     /gene="rpsA"
                     /locus_tag="ON345_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR3071325.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3557..3841
                     /gene="ihfB"
                     /locus_tag="ON345_001238"
     CDS             3557..3841
                     /gene="ihfB"
                     /locus_tag="ON345_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR3071326.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4048..6312
                     /locus_tag="ON345_001239"
     CDS             4048..6312
                     /locus_tag="ON345_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR3071327.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPRTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6349..8097
                     /gene="msbA"
                     /locus_tag="ON345_001240"
     CDS             6349..8097
                     /gene="msbA"
                     /locus_tag="ON345_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR3071328.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8094..9080
                     /gene="lpxK"
                     /locus_tag="ON345_001241"
     CDS             8094..9080
                     /gene="lpxK"
                     /locus_tag="ON345_001241"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR3071329.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGAEPAKLLTQLTSLASGN"
     gene            9117..10349
                     /locus_tag="ON345_001242"
     CDS             9117..10349
                     /locus_tag="ON345_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR3071330.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQCVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10401..10583
                     /gene="ycaR"
                     /locus_tag="ON345_001243"
     CDS             10401..10583
                     /gene="ycaR"
                     /locus_tag="ON345_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR3071331.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10580..11326
                     /gene="kdsB"
                     /locus_tag="ON345_001244"
     CDS             10580..11326
                     /gene="kdsB"
                     /locus_tag="ON345_001244"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR3071332.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIRNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            11480..12373
                     /locus_tag="ON345_001245"
     CDS             11480..12373
                     /locus_tag="ON345_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR3071333.1"
                     /translation="MEQLRAELSHLLGKKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12350..13129)
                     /gene="elyC"
                     /locus_tag="ON345_001246"
     CDS             complement(12350..13129)
                     /gene="elyC"
                     /locus_tag="ON345_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR3071334.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13265..14050
                     /gene="cmoM"
                     /locus_tag="ON345_001247"
     CDS             13265..14050
                     /gene="cmoM"
                     /locus_tag="ON345_001247"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR3071335.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETSVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14047..15369
                     /gene="mukF"
                     /locus_tag="ON345_001248"
     CDS             14047..15369
                     /gene="mukF"
                     /locus_tag="ON345_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR3071336.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15350..16054
                     /gene="mukE"
                     /locus_tag="ON345_001249"
     CDS             15350..16054
                     /gene="mukE"
                     /locus_tag="ON345_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR3071337.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            16054..20514
                     /gene="mukB"
                     /locus_tag="ON345_001250"
     CDS             16054..20514
                     /gene="mukB"
                     /locus_tag="ON345_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR3071338.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNNE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20775..22622
                     /gene="ldtD"
                     /locus_tag="ON345_001251"
     CDS             20775..22622
                     /gene="ldtD"
                     /locus_tag="ON345_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR3071339.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22803..23351
                     /locus_tag="ON345_001252"
     CDS             22803..23351
                     /locus_tag="ON345_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR3071340.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23378..24025
                     /gene="gloC"
                     /locus_tag="ON345_001253"
     CDS             23378..24025
                     /gene="gloC"
                     /locus_tag="ON345_001253"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR3071341.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24247..25437)
                     /gene="aspC"
                     /locus_tag="ON345_001254"
     CDS             complement(24247..25437)
                     /gene="aspC"
                     /locus_tag="ON345_001254"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR3071342.1"
                     /translation="MFENITTAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLALLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25622..26710)
                     /gene="ompF"
                     /locus_tag="ON345_001255"
     CDS             complement(25622..26710)
                     /gene="ompF"
                     /locus_tag="ON345_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR3071343.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6492 a         6581 c         7300 g         6478 t
ORIGIN      
        1 tactaaatta ctgctggcga ttgcgacctc tgcagcatta ctgacagggt gtcaaaatac
       61 ccacggtatt gataccaata tggctatcag ctccggttta aatgcctata aagcagcaac
      121 attaagcgat gccgatgcaa aagcgattgc caatcagggc tgtgccgaaa tggacagcgg
      181 caatcaagtc gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa tcgccaaagc
      241 attgggtaac aatattaatg gcacgccggt caactataag gtttatatga ccagcgacgt
      301 caacgcatgg gcgatggcga acggctgtgt ccgtgtctac agtggcctga tggacatgat
      361 gaatgacaac gaaattgaag gtgttctggg ccatgaactg ggccacgtcg cgttgggtca
      421 ctcgctggct gaaatgaaag cttcttatgc gatcgttgcc gcacgcgatg ccatttcagc
      481 taccagcggt gtggcttccc agctttcccg ctcacaattg ggtgatatcg cagaaggcgc
      541 tattaatgct aaatactccc gtgataaaga gtccgaagca gatgatttct cctttgatct
      601 gttgaagaaa cgtggcatca gcacccaggg gctggttggc agctttgaaa aactggctag
      661 tctggatggc ggtcgcaccc agtccatgtt tgactctcac ccaccatcaa cagagcgtgc
      721 gcaacacatc cgtgatcgta tcgcctctgg taagtaaatc attgtcatct ttcgggctgg
      781 tcttctgcca gcccgctata attgcgcaat aaatccccat ctgaatacag acaaaactgg
      841 tttttgcaca caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg cggttatgtt
      901 aacggtacgc ctgttttaag gagataaaga tgacggcaat tgccccggtt attaccattg
      961 atggcccaag cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa gcattgcaat
     1021 ggcatctgct ggattcgggt gcaatttatc gcgtactggc attggcggca ttacatcacc
     1081 atgttgatgt tgcgtcagaa gatgcgctgg taccgctggc atcccatctg gatgtacgtt
     1141 ttgtgtcaac caatggcaat ctggaagtga tcctcgaagg ggaagatgtc agcggcgaaa
     1201 ttcgtactca ggaagtggcg aatgcagctt cacaagtcgc ggcattccca cgcgttcgtg
     1261 aagcattatt gcgtcgccaa cgcgcgtttc gcgaattacc aggcctgatt gccgatggcc
     1321 gcgacatggg aacggtggta ttccctgatg cgccagtgaa aattttcctt gacgcctcct
     1381 cggaagaacg tgcgcatcgc cgcatgctac agttgcagga gaagggcttt agtgttaact
     1441 ttgagcgcct tttggccgag atcatagaac gcgacgaccg cgatcgtaac cgagcggtag
     1501 cgccactggt tccggcagcc gatgctttag tgttggattc caccacctta agcattgagc
     1561 aagtgattga aaaagcgcta caatacgcgc gccagaaatt ggctctcgca taagcgaccg
     1621 aatttgcagt acccccgttg caatggaatg acagcgggta tgttaaacaa ccccatccgg
     1681 catggagcca ggtggacgtt aaatataaac ctgaagatta aacatgactg aatcttttgc
     1741 tcaactcttt gaagagtcct taaaagaaat cgaaacccgc ccgggttcta tcgttcgtgg
     1801 cgttgttgtt gctatcgaca aagacgtagt actggttgac gctggtctga aatctgagtc
     1861 cgccatcccg gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc aggtaggtga
     1921 cgaagttgac gttgctctgg acgcagtaga agacggcttc ggtgaaactc tgctgtcccg
     1981 tgagaaagct aaacgtcacg aagcctggat cacgctggaa aaagcttacg aagatgctga
     2041 aactgttacc ggtgttatca acggcaaagt taagggcggc ttcactgttg agctgaacgg
     2101 tattcgtgcg ttcctgccag gttctctggt agacgttcgt ccggtgcgtg acactctgca
     2161 cctggaaggc aaagagcttg aattcaaagt aatcaagctg gatcagaagc gcaacaacgt
     2221 tgttgtttct cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg atcagctgct
     2281 ggaaaacctg caggaaggca tggaagttaa aggtatcgtt aagaacctca ctgactacgg
     2341 tgcattcgtt gatctgggcg gcgttgacgg cctgctgcac atcactgaca tggcctggaa
     2401 acgcgttaag catccgagcg aaatcgtcaa cgtgggcgac gaaatcactg ttaaagtgct
     2461 gaagttcgac cgcgaacgta cccgtgtatc cctgggcctg aaacagttgg gcgaagatcc
     2521 gtgggtagct atcgctaaac gttatccgga aggtaccaaa ctgactggtc gcgtgaccaa
     2581 cctgaccgac tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc tggtacacgt
     2641 ttccgaaatg gattggacca acaaaaacat ccacccgtcc aaagttgtta acgttggcga
     2701 tgtagtggaa gttatggttc tggatatcga cgaagaacgt cgtcgtatct ccctgggtct
     2761 gaaacagtgc aaagctaacc cgtggcagca gttcgcggaa acccacaaca agggcgaccg
     2821 tgttgaaggt aaaatcaagt ctatcactga cttcggtatc ttcatcggcc tggacggcgg
     2881 catcgacggc ctggttcacc tgtctgacat ctcctggaac gttgcaggcg aagaagcagt
     2941 tcgtgaatac aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg acgcagaacg
     3001 tgaacgtatc tccctgggcg ttaaacagct cgcagaagat ccgttcaaca actgggttgc
     3061 tctgaacaag aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg ctaaaggcgc
     3121 aaccgtagaa ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag catcccgtga
     3181 ccgcgttgaa gacgctaccc tggttctgag cgttggcgac gaagttgaag ctaaattcac
     3241 cggcgttgat cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag acgaagctga
     3301 cgagaaagat gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct ccaacaacgc
     3361 aatggctgaa gctttcaaag cagctaaagg cgagtaattc tctgactctt cgggattttt
     3421 attccgaagt ttgttgagtt tacttgacag attgcaggtt tcgtcctgta atcaagcact
     3481 aagggcggct acggccgccc ttaatcaatg cagcaacagc agccgcttaa tttgccttta
     3541 aggaaccgga ggaatcatga ccaagtcaga attgatagaa agacttgcca cccagcaatc
     3601 tcacattccc gccaagacgg ttgaagatgc agtaaaagag atgctggagc atatggcctc
     3661 gactcttgcg cagggcgagc gtattgaaat ccgcggtttc ggcagtttct ctttgcacta
     3721 ccgcgcacca cgtaccggac gtaatccgaa gactggcgat aaagtagaac tggaaggaaa
     3781 atacgttcct cactttaaac ctggtaaaga actgcgcgat cgcgccaata tttacggtta
     3841 agtttttact caaacttgaa cgcgagaaaa gcacctgtcg ggtgcttttt tcatttctct
     3901 aatcaggaac tggaagctgc ctcgcagagt tttgagcagt tttcaccctt tcgttaaatt
     3961 cttctgaatg tgcctcgggg aacgcaaaat tcccaccaga caaccgctca acaaagttgc
     4021 acactttcca taaacaggga ggggtgcatg aaaataacga cagtcggcgt atgcataatt
     4081 tgcggaattt ttccgttgct gattttgccc caattgccta ggacattaac ccttgcgttt
     4141 ctgactctct tcgcctgcgt actggcattt atccctgtta aaaccgtccg ttatatcgcg
     4201 ctgacgttgc tgtttttcgt ttggggcata ttatcagcaa agcaaatttt gtgggcagga
     4261 gaaaccttaa ctggcgcgac gcaggatgca attgttgaga tcactgcaac tgacggcatg
     4321 accactcatt acggtcaaat tactcatcta caaggtcgac gtatattccc tgcgccaggc
     4381 cttgtgctgt atggcgaata tcttccgcaa gcggtttgtg ctggacaaca atggtcaatg
     4441 aaactcaaag ttcgtgcagt tcatggccaa cttaatgatg gcggctttga tagccagcgt
     4501 tatgccattg cccagcatca gccgctcacc ggccgctttc tgcaggcaag tgtcattgaa
     4561 ccgaattgta gcctgcgtgc acagtatctg gcgtcactac aaacaacgct gcaaccctat
     4621 atgtggaatg cggttattct tggtttaggt atgggggaac ggttatccgt ccccaaagaa
     4681 atcaaaaata tcatgcgtga tactggaacg gcgcatttaa tggcgatatc gggattgcac
     4741 atcgcttttg cggcgttgct ggctgccgga ctcattcgcg gtgggcaagt ttatctgcct
     4801 gggcgctgga tccactggca aatgccatta attggtggaa tctgctgtgc tgctttttat
     4861 gcctggttga cgggaatgca acctcctgca ttgcgtaccg tggtggcgct tgctacgtgg
     4921 ggaatgctta agttaagtgg gcgacagtgg agtggctggg atgtatggat atgttgtctg
     4981 gcggcaattt tgctgatgga tcctgttgcc attctctcgc aaagtttatg gctctctgcc
     5041 gctgcggtcg cggcactgat tttttggtac cagtggtttc cctgtcctga gtggcaactg
     5101 ccgccggtat tgcgtgcagt tgtttccctc attcatttgc aactgggaat cacactcctg
     5161 cttatgcctg tgcaaatcgt catatttcat ggcattagtc tgacctcgtt tattgcaaat
     5221 ctattagcaa ttcccttggt gacatttatc acggttccgt tgatcctcgc cgcgatggtt
     5281 gtgcatttaa gcgggccgtt aatcctggag caagggttat ggtttcttgc cgaccggtct
     5341 ttggctttac ttttctgggg gttaaagagt ttgccagaag ggtggatcaa cattgctgaa
     5401 cgttggcaat ggctatcatt ttccccatgg ttcttactgg tggtatggcg attaaacgcc
     5461 tggcgaacgt tgccagcaat gtgtgtggct gtaggcttgc tgatgtgctg gccgctgtgg
     5521 caaaaacctc gacctgacga gtggcaggtg tacatgcttg atgtcgggca agggctggca
     5581 atggtgatag ccagaaacgg caaagcgatt ctctatgaca caggactggc ctggcccgaa
     5641 ggggatagtg ggcaacaact gattatcccc tggctccact ggcataatct tgaaccggaa
     5701 ggcgttattc tgagtcatga acatctggat caccggggag ggctggactc aatattgcac
     5761 acatggccga tgttatggat cagaagtccg ctaaactggg aacatcatca gccctgtgtg
     5821 cgtggcgaag cgtggcaatg gcaaggattg cgtttcagcg tgcactggcc tttacaagct
     5881 agcaacgata aaggaaataa ccattcctgt gtggttaagg ttgatgacgg gacgaatagc
     5941 attcttctaa ccggtgatat tgaagtcccc gctgaacaaa agatgctaag ccgttactgg
     6001 cagcaagtgc agacaacatt gcttcaggta cctcaccatg gcagtaatac ctcatcatcg
     6061 ttgccattaa ttcagcgagt gaatggaaaa gtggcactcg catcggcatc gcgctataac
     6121 gcatggcgat tgccctctaa taaagttaag catcgctatc aacagcaagg atatcaatgg
     6181 cttgatactc cacatcaggg tcaagtgacg gtcaattttt cagcgcaagg ctggcggatt
     6241 agcagcctca gagagcaaat tttacctcgt tggtatcatc agtggtttgg cgtgccagtg
     6301 gataacgggt agaatatgcg gctatttcaa caaatgctgg ttttttgaat gcataacgac
     6361 aaagatctct ctacgtggca gacgttccgc cgactgtggc caaccattgc gccttttaaa
     6421 gcgggtctga tcgtggcggg cgtagcgtta atcctcaacg cagccagcga taccttcatg
     6481 ttatcgctcc ttaagccact tcttgatgat ggctttggta aaacagatcg ctccgtgctg
     6541 gtgtggatgc cgctggtggt gatcgggctg atgattttac gtggtatcac cagctatgtc
     6601 tccagctact gtatctcctg ggtatcagga aaggtggtaa tgaccatgcg tcgccgcctg
     6661 tttggtcaca tgatgggaat gccggtttca ttttttgaca aacagtcaac ggggacgctg
     6721 ttgtcacgta tcacctacga ttccgaacag gttgcttctt cttcttccgg cgcactgata
     6781 acggttgtgc gtgaaggtgc gtcaatcatc ggcctgttca tcatgatgtt ctattacagt
     6841 tggcaactgt cgatcatttt gattgtgctg gcaccgattg tttcgattgc gattcgcgta
     6901 gtatcgaagc gttttcgcaa catcagtaaa aacatgcaga acaccatggg gcaggtgacc
     6961 accagcgccg aacaaatgct gaagggccac aaagaagtat tgattttcgg tggtcaggaa
     7021 gtggaaacga aacgctttga taaagtcagc aaccgaatgc gtcttcaggg gatgaaaatg
     7081 gtttcagcct cttccatctc tgatccgatc attcagctga tcgcctcttt ggcgctggcg
     7141 tttgttctgt atgcggcgag cttcccaagt gtcatggata gcctgactgc cggtacgatt
     7201 accgttgttt tctcttcaat gattgcactg atgcgtccgc tgaaatcact gaccaacgtt
     7261 aacgcccagt tccagcgcgg tatggcggct tgtcagacgc tgtttaccat tctggacagt
     7321 gagcaggaga aagacgaagg taagcgcgtg atcgagcgtg cgactggcga cgtggaattc
     7381 cgcaatgtca cctttactta tccgggacgt gacgtacctg cattgcgtaa catcaacctg
     7441 aaaattccgg cagggaagac ggttgctctg gttggacgct ctggttcagg taaatcaacc
     7501 atcgccagcc tgatcactcg tttttacgat attgatgaag gcgaaatcct gatggatggt
     7561 cacgatctgc gcgagtatac cctggcgtcg ttacgtaacc aggttgctct ggtgtcgcag
     7621 aatgtccatc tgtttaacga tacggttgct aacaacattg cttacgcacg gactgaacag
     7681 tacagccgtg agcaaattga agaagcggcg cgtatggcct acgccatgga cttcatcaat
     7741 aagatggata acggtctcga tacagtgatt ggtgaaaacg gcgtgctgct ctctggcggt
     7801 cagcgtcagc gtattgctat cgctcgagcc ttgctgcgtg atagcccgat tctgattctg
     7861 gatgaagcta cctcggctct ggataccgaa tccgaacgtg cgattcaggc ggcactggat
     7921 gagttgcaga aaaaccgtac ctctctggtg attgcccacc gcttgtctac cattgaaaag
     7981 gcagacgaaa tcgtggtcgt cgaggatggt gtcattgtgg aacgcggtac gcataacgat
     8041 ttgcttgagc accgcggcgt ttacgcgcaa cttcacaaaa tgcagtttgg ccaatgatcg
     8101 aaaaaatctg gtctggtgaa tcccctttgt ggcggctatt gctgccactc tcctggttgt
     8161 atggcctggt gagtggcgcg atccgtcttt gctataaact aaaactgaag cgcgcctggc
     8221 gtgcccccgt accggttgtc gtggttggta atctcaccgc aggcggcaac ggaaaaaccc
     8281 cggtcgttgt ctggctggtg gaacagttgc aacagcgcgg tattcgcgtg ggggtcgtat
     8341 cgaggggata tggtggtaag gctgaatctt atccgctgtt attgtcggca gataccacaa
     8401 cagcacaggc gggtgatgaa cctgtgttga tttatcaacg cactgatgcg cctgttgcgg
     8461 tttctcccgt tcgttctgat gcggtaaaag ccattctggc gcaacaccct gatgtgcaga
     8521 tcatcgtaac cgacgacggt ttacagcatt accgtctggc gcgtgatgtg gaaattgtcg
     8581 ttattgatgg tgtgcgtcgc tttggcaatg gctggtggtt gccggcgggg ccaatgcgtg
     8641 agcgagcggg gcgcttaaag tcggttgatg cggtaatcgt caacggcggt gtccctcgca
     8701 gcggtgaaat ccccatgcat ctgctgccgg gtcaggcggt gaatttacgt accggtacgc
     8761 gttgtgacgt tgctcagctt gaacatgtag tggcgatggc ggggattggg catccgccgc
     8821 gcttttttgc cacgctgaag atgtgtggcg tacaaccgga aaaatgtgta ccgctggccg
     8881 atcatcagtc tttgaaccat gcggatgtca gtgcgttggt aagcgccggg caaacgctgg
     8941 taatgactga aaaagatgcg gtgaaatgcc gggcctttgc agaagaaaat tggtggtatt
     9001 tgcctgtaga cgcacagctt tcaggtgctg aaccagcgaa actgcttacg caactaacct
     9061 cgctggcttc tggcaactag ttacgccgcg gcagcgttcg attgatggag tcatgaatgt
     9121 cgctgccgca cctctccctt gctgatgcgc gtaatcttca ccttgccgca caaggcctgt
     9181 taaacaaacc ccgccgtcga gcgtcgttgg aggatattcc ggcaacgatc tcccgcatgt
     9241 ccttgctgca aatcgatacc atcaatattg ttgcccgtag tccatatctg gtgcttttca
     9301 gtcgtctggg aaattatcct gcccagtggc tggatgagtc tctggcgcgt ggcgaattaa
     9361 tggaatactg ggcgcatgaa gcctgcttta tgccgcgtag cgactttcgt cttattcgcc
     9421 accgcatgct ggcacctgaa aaaatgggct ggaaatacaa agacgcctgg atgcaggaac
     9481 atgaggcgga aattgcacag ttaattcagc atattcatga taaggggccg gtacgttcag
     9541 ccgattttga gcatcctcgt aaaggtgcaa gcggctggtg ggaatggaag ccgcataaac
     9601 ggcatctgga aggtttattt actgccggaa aggtgatggt gattgaacgg cgcaacttcc
     9661 agtgcgttta tgatttaacc caccgtgtca tgcctgactg ggatgatgag cgcgatctcg
     9721 tttcgcaaac agaagcagaa atcatcatgc tggataacag tgcgcgtagc ctgggaatat
     9781 tccgcgaaca gtggctggca gattactatc ggctgaaacg tccggcactg gcggcgtggc
     9841 gcgaagcgag ggctgaacag cagcaaatca ttgctgtgca tgttgaaaaa ttgggcaatc
     9901 tttggctgca tgctgatttg ctgccgctac tcgagcgtgc gctggccgga aagctcactg
     9961 caacgcacag cgcggtactt tcgccttttg atcctgttgt ctgggatcgc aaacgcgcag
    10021 agcagctttt tgattttagc taccggctgg agtgctatac cccagcgccg aaacgccagt
    10081 atggctattt tgttctgcca ttattacatc gtgggcaatt agttgggcga atggatgcca
    10141 aaatgcatcg ccagacaggc atccttgaag ttatctctct gtggttacag gaaggcatta
    10201 aaccaacgac aatgctgcaa aaaggattac gtcaggcgat tactgatttc gcgagctggc
    10261 agcaggcaac gcgggtgaca ttaggacgct gcccgcaagg tctctttact gattgccgca
    10321 ctggctggga aatagacccc gtcgcataaa tgattatgat aagatttatg cattcataac
    10381 ccgatccatc tggaggaact atggatcatc gtctgcttga aatcattgcc tgcccggttt
    10441 gcaacggaaa actttggtat aaccaggaaa aacaagaact tatttgcaaa ctggacaacc
    10501 tggctttccc gctgcgtgat ggcattccag tgttgctgga aacggaagcc cgcgtgctga
    10561 ctgctgatga gagtaaatca tgagttttgt ggtcattatt cccgcgcgct acgcgtcaac
    10621 gcgtctgccc ggtaaaccat tggttgatat taatggcaaa cccatgattg ttcatgttct
    10681 tgaacgtgct cgcgaatcag gtgccgatcg catcatcgtg gcaaccgatc atgaggatgt
    10741 cgcccgcgcc gttgaagccg ctggcggtga agtatgtatg acgcgcgccg atcaccagtc
    10801 agggacagaa cgcctggcgg aagttgtcga aaaatgcgcg ttcagcgacg acacggtgat
    10861 cgttaatgtg cagggtgatg aaccgatgat ccctgcgaca atcattcgtc aggttgctga
    10921 taacctcgct cagcgtcagg tgggtatggc gactctggcg gtgccaatcc gcaatgcaga
    10981 agaagcgttt aacccgaatg cggtgaaagt ggttctcgac gctgaagggt atgcactgta
    11041 cttctcacgc gccaccattc cttgggatcg tgatcgtttt gcagaaggcc ttgaaaccgt
    11101 tggcgataac ttcctgcgtc atcttggtat ttatggctac cgtgcaggct ttatccgtcg
    11161 ttacgtcaac tggcaggcaa gtccgttaga acacatcgaa atgttagagc agcttcgtgt
    11221 tctgtggtac ggcgaaaaaa tccatgttgc tgttgcgcat gaagttcctg gcacaggtgt
    11281 ggatacccct gaagatctcg agcgcgttcg cgctgaaatg cgctaatttc acttcacgac
    11341 actccagcca attttgggag gagtgtcgta ccgttacgat tttcctcaat ttttcttttc
    11401 aacaattgat ctcattcagg tgacatcttt tatattggcg ctcattatga aagcagtagc
    11461 ttttatgagg gtaatctgaa tggaacagct gcgtgccgaa ttaagccatt tactgggcaa
    11521 aaaactcagt cgtattgagt gcgtcaatga aaaagcggat acggcgttgt gggctttgta
    11581 tgacagccag ggaaacccaa tgccgttaat ggcaagaagc tttagtacgc ccggaaaagc
    11641 ccgacaactg gcatggaaaa ccaccatgct ggcaagaagt gggactgtcc gtatgccgac
    11701 tatttatggt gtgatgacgc atgaagaaca ccccggcccc gatgtcctgc tactggagcg
    11761 gatgcgtggt gtttcggtgg aggcaccagc ccgaacacca gaacgctggg aacaactcaa
    11821 agaccaaatc gttgaagcct tactggcctg gcaccgtcag gacagtcgcg gttgcgtcgg
    11881 cgcggtcgac aatactcagg aaaatttctg gccctcatgg taccggcaac atgttgaagt
    11941 gctatggacc acgctcaatc agttcaataa caccggtctg acgatgcagg ataagcggat
    12001 cctgtttcgc actcgcgaat gtctcccggc attatttgaa ggctttaacg acaattgtgt
    12061 gctgattcac ggtaacttct gtttacgcag catgttgaaa gattcgcgca gcgatcagtt
    12121 actggcgatg gtcgggccgg gactaatgct ttgggcaccg cgagaatacg aactgttccg
    12181 actaatggat aattctctgg cggaagattt gctctggagt tacctgcaac gcgcgccagt
    12241 ggcggagtcg ttcatctggc ggcgttggtt gtatgtgtta tgggatgaag ttgcgcaact
    12301 ggttaatacc ggacgattta gtcggcgcaa cttcgatctg gcatcaaaat cactcttgcc
    12361 gtggctcgcc tgacgaacct tttagccact gccagatacg gccaagcgtt tcatagccaa
    12421 cacgatcgct atgcatcaac cagaccggag aagggattgc ccgttcccac gggttaagcg
    12481 gcgagtcgat ggccaactga ttcgccgggg ctggtagcgg atttaaccct tcctgctgaa
    12541 aaaagatcat cgcccgcggc agatgtgatg cggaggtcac cagcaggaaa ggggcgtcac
    12601 caatcgcctg tttcactgcc gcagcttctt cttcggtatc ttttggcaaa tccagggtga
    12661 taatttgctc gcgcggcacg cccagcgatt gcgcaactct ggcacctact tccgctgtac
    12721 tcaccgtatt ggtttttgct acgcctcccg tgaagatcag ttttgatccc ggattttcgc
    12781 gccataagcg aataccttca ttcaggcgtg gcagactgtt attgattaaa ttagagctcg
    12841 gtgcccactg cgggttccag gtataaccac ctccgagcac cacgatatag tccacttttt
    12901 gcgaattatt ccatgtcgga taggtgcttt cgatgggacg cagcaggcgg tcggctaccg
    12961 gttgcaaact caataacaag agtgccagcc acccaatgct aataaaaatc ttcgcggttt
    13021 tttgaaaacg gctaaaccac agcagcgcca ggccagcgcc aataattaac aacatcagcg
    13081 gaagtggcag caacatattg ccaatcactt ttttcagtgt aaaaagcatc ctttctggtt
    13141 ccttttttaa ccatatagca agggatcgcc agcgatatta caccagacgg gttcattctc
    13201 gccgtggctg tgacaaaata gcgtctttat cagaaaacgt cttagccaac cggtggagaa
    13261 agcaatgcag gatcgcaatt ttgatgatat tgcggaaaag ttttcccgta acatttacgg
    13321 caccaccaaa gggcagcttc gacaggctat tctgtggcag gatctcgatc gcgtgctggc
    13381 ggaaatgggt ccgcaaaaac tgcgtgtgct ggatgcgggc ggtggagaag ggcagaccgc
    13441 aatcaaaatg gccgagcgcg ggcatcaggt cattttatgc gatctttctg cgcagatgat
    13501 cgaccgcgca aaacaggcgg cagaagcaaa aggtgtgagc gacaacatgc aatttataca
    13561 ttgcgccgct caggatgttg cttcgcattt ggaaacgtcc gttgatctga tattgttcca
    13621 tgcggtgctc gagtgggtgg ccgatccccg cagtgtattg cagaccctgt ggtcagtgtt
    13681 gcgtccaggc ggcgtgttgt cgttaatgtt ctacaatgcg catggtttgt tgatgcataa
    13741 catggtcgcc gggaattttg attacgtgca ggcgggaatg ccgaaaaaga aaaaacggac
    13801 gctttcgcca gattatccac gcgacccggc gcaggtttat ctgtggctgg aagaagctgg
    13861 ttggcaaatt atgggtaaga caggcgttcg cgtgtttcat gattatctgc gcgagaagca
    13921 ccagcagcgc gactgctatg aagcattact tgaattagaa acgcgttatt gccgtcagga
    13981 accgtatatt accctggggc gttatattca tgtcaccgcg cgcaaaccgc agagcaagga
    14041 taaagtatga gtgaattttc ccagacagtc cccgaactgg ttgcctgggc cagaaaaaat
    14101 gacttctcca tctcgctgcc ggtagaccga ctctcttttc tgctggcggt tgccacgctg
    14161 aacggcgagc gtctggatgg tgagatgagt gaaggcgagc tggtggatgc attccgccat
    14221 gtgagtgatg cgtttgagca aaccagcgaa accatcggcg tgcgcgccaa taacgcgatc
    14281 aacgacatgg tgcgtcaacg tctgctgaac cgctttacca gcgagcaggc ggaagggaac
    14341 gcaatttacc gtctgacgcc gctcggcatc ggcattactg actactacat ccgtcagcgc
    14401 gagttttcta cgctgcgtct ttctatgcag ttgtcgattg tggcgggtga gctcaaacgc
    14461 gcagcagatg ccgccgaaga gggcggtgat gaatttcact ggcaccgtaa tgtctatgcg
    14521 ccactgaaat attcggtagc agaaattttc gacagtatcg acctgacgca acgtctgatg
    14581 gacgaacagc agcagcaggt gaaggacgat atcgcccagt tgctgaacaa agactggcgg
    14641 gcggcgattt ccagctgtga attgttgctt tcggaaactt ccggaacgct gcgtgaattg
    14701 caggatacgc tggaagcggc aggcgacaaa ttgcaggcta atctgttgcg cattcaggat
    14761 gcgacgatga cccatgacga tctgcatttc gtcgatcgtc tggtgttcga tctgcagagc
    14821 aaactcgatc gtattatcag ttggggccag caatccatcg acttgtggat tggctacgac
    14881 cgccacgtac acaaatttat tcgtaccgcg atcgatatgg ataaaaaccg cgtctttgct
    14941 cagcggttac gtcagtcggt acaaacctat tttgatgagc cgtgggcgct aacttatgcc
    15001 aatgccgatc gtctgctgga tatgcgtgac gaagagatgg cactgcgcga tgaagaagtg
    15061 actggggaac ttcctgagga tctggaatac gaagagttta acgagatccg cgaacagctg
    15121 gcggcgatca tcgaagaaca acttgccgtg tacaaaacca gacaagtgcc gctggatctt
    15181 ggtctggtgg tacgcgaata tctgtcacag tatccgcgtg cacgtcactt tgacgttgcg
    15241 cgtattgtta ttgatcaggc ggtacgtctt ggcgtagcgc aagcagattt caccggactg
    15301 ccagcgaaat ggcagccgat taatgattac ggagccaagg tacaggcgca tgtcatcgac
    15361 aaatattgaa caagtgatgc cggttaagct ggcgcaggcg ctggcgaatc cgttatttcc
    15421 ggcgctggac agcgccttac gttcaggccg ccatattggc ctcgacgaac tggataatca
    15481 tgcattcctg atggattttc aggaatatct ggaagagttt tacgcgcgtt ataacgttga
    15541 gcttattcgc gcaccggaag ggttcttcta tttacgccca cgttccacca ctcttatccc
    15601 gcgttccgtc ttgtcggaac tggatatgat ggtcgggaaa atcctctgtt atctctatct
    15661 cagcccggaa cggctggcga atgaggggat tttcacccag caggaactgt acgacgaact
    15721 gctcaccctg gccgatgaag caaaactgct gaaactggtg aacaaccgtt caaccggttc
    15781 agacgttgac cgtcagaagt tgcaggagaa agtacgttct tcgctcaacc gtctgcgtcg
    15841 tttaggcatg gtgtggttta tgggccacga cagcagcaag tttcgcatta ccgaatcggt
    15901 gttccgcttc ggagccgatg tgcgtgctgg cgacgatccc cgtgaagcac agcgtcgcct
    15961 gattcgtgat ggcgaagcaa tgccgattga aaatcatctg caactcaacg atgaaaccga
    16021 agagaatcag ccagatagcg gagaggaaga ataatgattg aacgcggtaa atttcgctca
    16081 ctgacgctga ttaactggaa cggctttttt gcccgaactt ttgaccttga cgagctggtc
    16141 acgacgcttt ctggcggtaa cggggcgggt aaatccacca ccatggcggc gttcgttacg
    16201 gcgctgatcc ccgacctgac cctgctgcat ttccgtaaca ctacggaagc cggggccacc
    16261 agcggttcgc gcgataaagg tctgcacggt aagctgaaag cgggtgtctg ttattcgatg
    16321 ctcgacacca ttaactcgcg ccaccagcgc gtggtggtcg gtgtgcgtct gcaacaggtt
    16381 gccggacgcg atcgtaaagt ggatatcaaa ccatttgcca ttcagggact gccgatgtcg
    16441 gtgcagccga cacagctggt gaccgaaacc ctgaacgaac gccaggcgcg cgtgctgccg
    16501 cttaacgagc tgaaagacaa gctcgaggcg atggaaggcg tgcagtttaa acagttcaac
    16561 tccattaccg attaccactc gctgatgttc gatctgggca tcatcgcgcg tcgtctgcgc
    16621 tccgcatctg accgtagcaa attctatcgt ctgatcgaag cttcgctgta tggcgggatc
    16681 tccagtgcca ttacccgttc tctgcgcgac tacctgttgc cggaaaacag cggcgtgcgt
    16741 aaagcgttcc aggacatgga agcggcgctg cgtgaaaacc gcatgacgct ggaagcgatt
    16801 cgtgtcaccc agtcggaccg tgacctgttt aagcatctga tcagcgaagc taccaactac
    16861 gtggcggcgg actacatgcg tcacgccaac gagcgccgtg tccatctcga caaagccctg
    16921 gagtttcgtc gcgagttaca tacttcgcgt cagcaactgg cggctgagca gtacaaacac
    16981 gtcgatatgg cgcgtgagct ggcagagcac aacggtgccg aaggcgatct ggaagcggat
    17041 tatcaggcgg ccagcgatca cctgaacctg gtgcaaaccg cactgcgtca acaggagaag
    17101 atcgaacgct acgaagcgga tctcgatgag ctgcagatcc gtctggaaga gcaaaacgaa
    17161 gtggtggcag aagccatcga acgccaggaa gagaacgagg ctcgcgcgga agctgccgaa
    17221 ctggaagtgg acgagctgaa aagccagctt gctgactacc agcaggcgct ggacgtccag
    17281 caaacgcgcg cgatccagta taaccaggcg attgctgcgc ttaatcgcgc caaagaactg
    17341 tgccatctgc cggacttaac cgccgacagc gccgccgaat ggctggaaac cttccaggcg
    17401 aaagagctgg aagcgactga aaaaatgctc tctcttgagc agaaaatgag catggcgcaa
    17461 accgcgcaca gccagtttga gcaggcttat cagctggtgg tggcaatcaa cggcccgctg
    17521 gcgcgtaacg aggcttggga tgtcgcccgc gaactgctgc gcgaaggggt cgatcagcgt
    17581 cacctggcag agcaggttca accgttgcgg atgcgcttaa gtgaactgga acagcgtctg
    17641 cgcgagcagc aagaagccga acgcctgctg gcagacttct gcaaacgtca gggcaagaat
    17701 tttgatattg atgaactgga agctctgcat caggagctgg aagcgcgcat tgcctctctt
    17761 tccgatagcg tgtctaacgc ccgtgaagag cgcatggcac tgcgccagga acaggaacag
    17821 ctgcagtctc gcattcagag tttgatgcag cgtgcgccgg tttggctggc agcgcaaaac
    17881 agtctcaacc agttgagcga gcagtgcggt gaagagttta cctccagcca ggacgtcacc
    17941 gaatatctgc aacagttgct ggagcgtgag cgagaggcga ttgttgaacg cgatgaagtg
    18001 ggcgcgcgca aaaacgctgt cgatgaagag atcgaacgtt taagccagcc tggcggctct
    18061 gaagatcagc gtctgaacgc gctggcggag cgttttggtg gtgtgctgct gtcagaaatt
    18121 tatgacgacg ttagcctgga agatgcgccg tacttctcag cgctgtatgg cccgtcacgc
    18181 cacgccatcg tggtgccaga tctgtcacag gtaactgaac acctggaagg cttgaccgat
    18241 tgcccggaag atctctattt gatcgaaggg gatccgcagt cattcgatga cagcgtgttc
    18301 agcgttgatg agctggaaaa agcggtagtg gtgaaaatcg ccgatcgtca gtggcgttat
    18361 tcacgtttcc cggaagtgcc gctgtttggt cgtgctgcgc gtgaaagccg tattgaaagc
    18421 ctccatgccg agcgtgaagt gctttccgaa cgcttcgcca cgctctcctt tgatgtacag
    18481 aaaacccagc gtctgcatca ggcgttcagc cgctttatcg gcagtcatct ggcggtggcg
    18541 tttgagtctg acccggaagc agaaatccgt caactgaaca gccgtcgcgt cgaactggag
    18601 cgggcgttga gtaataatga aaatgataac cagcagcagc gtattcagtt tgagcaggcg
    18661 aaagaaggcg ttacggcgct gaaccgcatt ctgccgcgtc tcaacctgtt ggctgatgac
    18721 agcctggcgg atcgcgtcga tgaaatccgc gaacgtctgg atgaagccca ggaagccgcg
    18781 cgttttgttc agcagtttgg caatcaactg gcgaaactgg aaccgatcgt ttcggtattg
    18841 cagagcgacc cggaacagtt cgaacagcta aaagaagatt acgcgtactc tcagcagatg
    18901 cagcgcgatg cccgtcagca ggcgtttgcc ctgacggaag tggtgcagcg tcgtgcgcac
    18961 tttagctatt ctgactcggc agaaatgctg agcggtaata gcgatctcaa cgaaaagctg
    19021 cgtgaacgtc tggaacaggc ggaagcggag cgtactcgcg ctcgtgaagc gttgcgtggt
    19081 cacgcagcgc agttgagtca gtacaaccag gtgctggctt cgctgaaaag ttcttacgac
    19141 accaaaaaag agctactcaa cgatctacaa cgtgaattgc aggatatcgg cgtgcgcgcc
    19201 gatagcgggg cagaagagcg ggcgcgaatt cgccgtgacg agctgcatgc gcaactgagt
    19261 aataaccggt cccgccgcaa ccagctggaa aaagcactga ctttctgcga agcggagatg
    19321 gacaatctga cccgcaaact gcgcaagctg gagcgggatt actttgagat gcgcgagcag
    19381 gtagtgaccg ccaaagcggg ctggtgtgca gtgatgcgca tggtgaaaga taacggcgtt
    19441 gaacggcgct tacaccgtcg cgagctggct tatctctccg ctgatgattt gcgctctatg
    19501 tcggataagg cattgggtgc gctgcgtctg gcggtggcgg ataacgaaca tctgcgcgac
    19561 gtgctgcgca tgtcggaaga tccaaaacgt ccggaacgta aaattcagtt cttcgtggcg
    19621 gtttatcagc atctgcgtga acgtattcgt caggatatta ttcgtaccga tgatccggtg
    19681 gaagccatcg agcagatgga aatcgaactt agccgactga ccgaggagtt aacctcccgc
    19741 gaacagaaac tggcgatcag ttcccgcagc gtggcgaaca tcattcgcaa aaccattcag
    19801 cgcgagcaga accgtatccg tatgctcaac caggggttgc agaacgtatc gtttggtcag
    19861 gtgaacagcg tacgtctgaa cgtgaacgtg cgtgaaacgc acgccatgct gctggatgtg
    19921 ctctctgaac agcacgagca gcatcaggat ctgtttaaca gcaaccgtct gaccttctcg
    19981 gaagcgctgg cgaaattgta tcagcgtctt aatccgcaga ttgatatggg gcagcgcacg
    20041 ccgcagacca ttggcgaaga actgctggat taccgcaact atctggaaat ggaagttgag
    20101 gttaaccgtg gttccgatgg ctggttgcgc gcagagtctg gtgcattgtc gaccggtgag
    20161 gcgattggta ccggtatgtc gattctggtg atggtggtac aaagctggga agatgaatct
    20221 cgccgcctgc gcggtaaaga tatctctcct tgccgcctgc tgttcctcga tgaagcagcg
    20281 cgactggatg ctcgttctat cgccacgctg tttgaattgt gtgaacgttt gcaaatgcag
    20341 ctcatcatcg cagcgccgga aaatatcagc ccggagaaag gcaccaccta taaactggtg
    20401 cgtaaagtct tccagaatac cgaacacgtt cacgtcgtcg gcctgcgagg atttgcgccg
    20461 caactccctg aaacgcttcc aggaagtgac gaagcgcctt ctcaggcgag ttaaaattaa
    20521 ggcggcagca atgccgcctt ttctttttcc gaaaactccg tttctgtact aaaaaagtgg
    20581 cacattacgg cgcgcttttc tttaaacttc tttacattag gttatgtaaa aacgtatcgg
    20641 cgtttatata ctgaagataa gcctgatgag taacaggctt gctcgtcata ctttcgtgag
    20701 tattggcgtt gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag tatgataaaa
    20761 acagggggca agggatgttg cttaatatga tgtgtggtcg tcggctgtcg gcaatcagtt
    20821 tgtgcctggc cgtaacattc gctccactgt tcaatgcgca ggccgatgag cctgaagtaa
    20881 tccctggcga cagcccggtg gctgtcagtg aacagggcga ggcactgccg caggcgcaag
    20941 ccacggcaat aatggcgggg atccagccat tgcctgaagg tgcggcagaa aaagcccgca
    21001 cgcaaatcga atctcaatta cccgcaggtt acaagccggt ttatcttaac cagcttcaac
    21061 tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct
    21121 tccagcaaca gctggcagag gtggcgattg ccggtttcca gccgcagttt aataaatggg
    21181 tagagttact gaccgatcct ggtgttaacg ggatggcacg cgacgtagtg ctctctgatg
    21241 cgatgatggg ctatctccat ttcattgcaa atattccggt caaaggcact cgctggctat
    21301 atagcagtaa accttatgca cttgcaacgc cgccgctctc ggtgattaac caatggcagc
    21361 tggcgctgga taaaggtcaa ttgcctacgt ttgttgcagg actggcaccg cagcatccgc
    21421 aatatgcggc gatgcatgaa tcgttactgg ccttactcag tgacaccaaa ccgtggcccc
    21481 aactgaccgg caaagcaacg ttgcgcccag ggcagtggag taacgacgta ccggcgttgc
    21541 gcgaaatatt gcaacgcaca ggcatgttgg acggggggcc gaaaattact ctacctggcg
    21601 atgacacgcc aactgacgcg gtagtcagcc catccgctgt tactgttgaa acagcagaaa
    21661 ctaagccgat ggacaagcaa acgacgtctc gtagtaaacc tgcgcctgcc gttcgtgccg
    21721 cctacgataa tgaactggtg gaagccgtta aacgttttca ggcatggcaa ggattggggg
    21781 cagatggtgc tattggcccg gcaacacgtg actggttaaa cgtaacgccc gcccagcgtg
    21841 ctggcgtgtt ggctctcaac atccagcgat tgcgcttgct gccaacagag ctttctaccg
    21901 ggatcatggt taacattccg gcctattcgc tggtctacta tcagaacggc aatcaggtgc
    21961 tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg agcagtgccc
    22021 ttaacaacgt agtggtaaac ccgccgtgga acgtacctcc aactctggca cgcaaagata
    22081 ttctgccaaa agtgcgcaac gatccgggat atctcgaaag ccatggctat acggtgatgc
    22141 gcggctggaa cagcagagaa gcgattgacc catggcaggt tgactggtct acaatcacgg
    22201 cctcgaattt accgttccgc ttccagcagg ctccaggccc acggaactcg ctggggcgct
    22261 ataaattcaa tatgccgagt tcagaggcca tttatttgca tgacacgccg aaccacaatc
    22321 tgttcaagcg tgatacacgc gcattgagct caggctgtgt acgagtgaat aaagcttccg
    22381 atctggcgaa tatgctgttg caggatgcag gctggaatga caaacgtatt tctgatgcgc
    22441 tgaagcaggg tgatacacgt tacgtcaata ttcggcagtc gattccggtg aatctctact
    22501 acctgacggc ctttgttggt gcagatggtc gtacccagta tcgtacagat atttacaatt
    22561 atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt
    22621 aaatgaagta gttcggggaa atcagttgtc gtaataatta gcatgattgg gggcgattct
    22681 ctgcagcccc cgtcactgct ggggttgagt catcttgacg tctgctttac gggcggttaa
    22741 ggtgcctctt gtgcgccaga agtgcatata aacgataaca ttgacctgta gacttgatta
    22801 tcatggacaa attcgacgct aatcgccgca aattgctggc gcttggtggc gttgcactcg
    22861 gtgccgccat cctgccgacc cctgcgtttg caacactctc taccccacgc ccgcgcattt
    22921 tgacactcaa taatcttcat accggagagt caatcaaagc ggagtttttc gatggcagag
    22981 gctatattca ggaagaattg gcaaaactta accatttttt ccgcgattac cgcgcgaaca
    23041 aaataaagtc catcgaccca ggattattcg accagttgta tcgcctgcaa gggttgttag
    23101 gcacgcgcaa accggtgcaa ctcatttccg gttatcgttc tattgatacc aacaatgaac
    23161 tacgcgcccg cagccgtgga gtagcgaaga aaagctatca cactaaaggc caggcgatgg
    23221 atttccatat tgaaggtatc gcgttaagca atattcgcaa agccgcgtta tctatgcgcg
    23281 caggtggtgt aggatattat ccacgtagta actttgtgca tattgatacc gggccagcac
    23341 ggcactggta gtaattgctt aacgaaacag gggcagtatg aactatcgta ttattccggt
    23401 caccgcattc tcccagaact gttcattaat ctggtgtgaa caaacccgtc tggccgcact
    23461 ggtcgatcct ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca gcggcctgac
    23521 actgatgcag atcctgctga cgcatggtca tctggaccac gttggcgcag cggcggaact
    23581 ggcgcaacat tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt tctggctgca
    23641 aggcttgcct gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc tgacgccaga
    23701 tcgttggctg aacgaaggcg ataccatcag catagggaat gtgactttac aggtgttaca
    23761 ttgccctggg catacgccgg gtcatgtcgt gttttttgat gatcgggcaa agctgctgat
    23821 ttctggcgat gttattttca aaggcggagt agggcgcagt gacttcccgc gtggcgatca
    23881 taatcaactg atttcttcaa tcaaagataa attgctgcca ctgggggatg acgtgacatt
    23941 tattccgggt cacggaccat tatccacact tggttatgaa cgcctgcata atcccttcct
    24001 gcaagacgaa atgcccgtct ggtaaggcac ataaaaaagc ccgcttttaa tgcgggcctg
    24061 gatttctggc aaagtgcgct ttgtttatgc cggatgcggc acgagcgcct tatccggcct
    24121 acaaaatcgt gcaaattcaa aatattgcag gggacgcgta ggcctgataa gcgtagcgca
    24181 tcaggcaatg ttgcgtttgt catcagtctc agcccgcttt tcagcgggct tcattgtttt
    24241 taatgcttac agcactgcca caatcgcttc gcacagcgga gccatgttat ctggtgtcat
    24301 cccggccaca tttacgcgac cagaagcaac cgcatatacg ccaaactctt cgcgcagacg
    24361 cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa
    24421 gctgaagtcg cggtttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg
    24481 ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta acgcatcgtt
    24541 gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcgcg
    24601 aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg cagcaaccag
    24661 agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac
    24721 aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc
    24781 acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caactgagag
    24841 tagtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt tatggcagca
    24901 gccatggaac agcactacgt cgccagcctg agcttcgttc aggctgttaa tcagtgcatc
    24961 gaagtcaagg gtgtggtttt ctgcatcata ataagcgtat tcacgaactt ccagacctgc
    25021 agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca ctcgcttaac
    25081 gctggtattt tttgccagga aatcggcagc tacgcgtagt gcgccagtgc cccccggagt
    25141 ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc
    25201 ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc
    25261 attttcgagc agatactgtt cagccttttt cacgctggtc aataccgggg ttttgcccgt
    25321 ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa
    25381 cagatcggcc aggcccagaa tcgggtcggc aggagcggtg gtaatgttct caaacatgac
    25441 gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa
    25501 attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga
    25561 aggcataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct
    25621 attagaactg gtaaacgata cccacagcaa cggtgtcgtc agaacctacg cccagtttgt
    25681 tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt
    25741 agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt
    25801 ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg aactggtatt
    25861 gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctgatgttt gtaaatttat
    25921 tagtgatcgg cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg
    25981 cgtcatactt cagaccagta gcccactgtt cagctttttt accgttgcca agaagttgag
    26041 cttcttgcag gttggtacgg tcagctgcac cataagcacc aacgatacca aagccttcgt
    26101 attcgtagct gatagaaccg ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct
    26161 cgtttttacc caggtactga acagcgaagt tcaggccatc aaccagacca aagaagttgg
    26221 agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaagtcatca ctgtatgcag
    26281 tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt
    26341 agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac
    26401 gcgttttgtt accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt
    26461 cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac
    26521 gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggagaaat
    26581 aatgcagacc gacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt
    26641 ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc
    26701 gcttcatcat tatttattac cctcatggtt ttttttatga cacctgccac tgccgtcaat
    26761 aagttctgtc aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct
    26821 gcaggcatct ttccattcaa actaacgttt c
//
LOCUS       DAJSKJ010000034        26638 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000034 DAJSKJ010000000
VERSION     DAJSKJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.034"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(163..777)
                     /locus_tag="ON345_001256"
     CDS             complement(163..777)
                     /locus_tag="ON345_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3071344.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKRE
                     ERPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATID
                     TTTAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMA
                     SMGQSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
     gene            939..1121
                     /locus_tag="ON345_001257"
                     /pseudo
     CDS             939..1121
                     /locus_tag="ON345_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            1212..1802
                     /locus_tag="ON345_001258"
     CDS             1212..1802
                     /locus_tag="ON345_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR3071345.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
     gene            1939..2511
                     /locus_tag="ON345_001259"
     CDS             1939..2511
                     /locus_tag="ON345_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3071346.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            2548..3939
                     /locus_tag="ON345_001260"
     CDS             2548..3939
                     /locus_tag="ON345_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HCR3071347.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLTVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(4914..5099)
                     /locus_tag="ON345_001261"
     CDS             complement(4914..5099)
                     /locus_tag="ON345_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071348.1"
                     /translation="MDRKSNSDSYQRWMIEKADKRERTQISMSFVKMKDVLFNVLAIG
                     TVLAALVVIFYEVQSSL"
     gene            complement(5057..5269)
                     /locus_tag="ON345_001262"
     CDS             complement(5057..5269)
                     /locus_tag="ON345_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071349.1"
                     /translation="MVTQSLADFGRYSYKPRPAFQDWIERLKTGERLPRGRYFVGKKP
                     GGRLVLIDELGGTWTEKVTVIHTNAG"
     gene            complement(5625..5879)
                     /locus_tag="ON345_001263"
     CDS             complement(5625..5879)
                     /locus_tag="ON345_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071350.1"
                     /translation="MGHYTIRTNDDEDQAIKKAQEATGQASASKTFMTAILELQRNRD
                     EMAQLRRELAQEKARSQELVSSVKQFRSSLNNLFDLADNP"
     gene            complement(5989..6810)
                     /locus_tag="ON345_001264"
     CDS             complement(5989..6810)
                     /locus_tag="ON345_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HCR3071351.1"
                     /translation="MTRATERAYSELQQAFDFYNQRLFDGELPDCLITFQRGKNTMGY
                     FSYRRFVAADGSGRMIDEIALNPEYFPVYPLIEVMQTLVHEQCHMWQYHYGNPSRKTY
                     HNAQWAAKMESIGLMPSSTGRPGGAKVGQKINDYPIPGGRFQRVTLELFQGQFALSWF
                     DRFPVQVEQQKDMTAVIEQWRETLALAHQNAEAGIDIEAVLSMALLPSVSPQNGSHSD
                     DMAGSNDNDLSVAAFEDKPKRNKVKYQCRGCGAAVWGKAGLNIECGDCELAFIEN"
     gene            complement(6807..6986)
                     /locus_tag="ON345_001265"
     CDS             complement(6807..6986)
                     /locus_tag="ON345_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071352.1"
                     /translation="MTEKITDEELADLLEALKRAHGMGVCSKAVKLAQRCADVFPAIV
                     AELQEYRNAAKRTSA"
     gene            7259..7909
                     /locus_tag="ON345_001266"
     CDS             7259..7909
                     /locus_tag="ON345_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR3071353.1"
                     /translation="MDIKPNSVIHLEPGETLPGYTNLKPVPDKYFDELKALINKLNTQ
                     SGNIYLKLKQMYAFLDRFNKEFVSTFTSCQKGCSSCCKMDVHLTALEATHIAQASKLT
                     ARDNPLTTGHESKCPFLSEKGTCSIYNYRPLLCRTYHVLTPPEMCNDLDAQVMQYGSQ
                     SANMGNHIYKTIAEWIYFQTYHCTGKLETKDIRDYFPYPREDIQRFLHHNPPRPFC"
     gene            complement(7947..8402)
                     /locus_tag="ON345_001267"
     CDS             complement(7947..8402)
                     /locus_tag="ON345_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HCR3071354.1"
                     /translation="MSENETFDATKKLLLNIRSVRVFARETSFEQLLEMQEKLNAVIE
                     ERREEAEREAAERAERERKRQELLQLIAGEGFSPEELLGLSEDAQKTRKKTLPKAPPK
                     YQFDENGETKYWSGRGRTPKPIDEALKAGRSLDEFLIKKDASSTAGDEQ"
     gene            complement(8414..10741)
                     /gene="topB"
                     /locus_tag="ON345_001268"
     CDS             complement(8414..10741)
                     /gene="topB"
                     /locus_tag="ON345_001268"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3071355.1"
                     /translation="MRLFIAEKPSLAKAIFEGLGGNPNTEKKNGYFEHGSDVVTWCYG
                     HMLELYDPQDYDDKYSNWRYSDLPIKAVYPPKYKIKPESEAQTRIILSLIDKADSIVH
                     SGDPDDEGCLLVDEILGYAGNKKPVQRLLVADLNLAPVQKSLSNLQPNDKFRGMTLSA
                     LARSLCDQGFGYNMTRGCTLKGREKGYDGVLNVGRVQSAVLGLVNMRTLANQNHTESF
                     YYDVFASLSMNGHQIKAKYQTTDDDQIDEKKRLISEAQAAHIAGRVTGKDAIVTIATT
                     KPENSKPPLPLNLSTLQQLCAKRFGYSAKDTLDVMQGLYETHKLLTYPRSDNRYLSDE
                     HYYQAGDIATAIAATVPELASAIVDMDKGQKHKAFNASKIEAHHAIIPTTKSGAGIQL
                     NEKERNVYNLVSVYFIGLFYPDAIRNKTKIHFDIKGDTFTATQSVLVQKGWEALGKDS
                     DDEDETEDAGTDGFDLSLLKFNDSGLCESADVDKKKTTPPRYFTESTLLAAMTSAAKF
                     IDDPALRKALEAKDEGSSDRGSIGTEATRAGILEKLAANTGLISIEKEKGYSELVWKT
                     TKQGQEFCAALPPEITKPDISALWAEKQSQIKAGELTVEEFIKENDEYVQGLIDELDR
                     NGISISSNATPCPVCNNGFLRKRKGQNGFFWGCSCYPECKTTFPDKDGKPDMEAKSRS
                     EGSMSRLEAPCPSCSKEIIIRPKGFFCSGCEFKIWSEVSGKKLTQNQVETLIKKGKTG
                     EIKGFTSNKTGKKFDAAIVLQDKTTGKLGFQFSKK"
     gene            complement(10745..12007)
                     /locus_tag="ON345_001269"
     CDS             complement(10745..12007)
                     /locus_tag="ON345_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3071356.1"
                     /translation="MGKLTLVEQALRLHKEYYGEDNFGTVVKDCDYYPKVAEIFINRK
                     NRKIYSNLDIEPVASTVVHTGSSPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIR
                     FILNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKIGCYDGVYYHGKAR
                     KEEIENALRFSVHPKFNELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIA
                     KALGAKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGA
                     DTPRNLAGTYVIFSTNYPNRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPDDI
                     NIDLKELGAVLDRTTPAEIKEIINIAIGMIRGTKNELTVDVIKNARAFLKGTLDLSVQ
                     EAEEDIEEREEIFKKNGAQPDYSSYLED"
     gene            complement(12090..12584)
                     /locus_tag="ON345_001270"
     CDS             complement(12090..12584)
                     /locus_tag="ON345_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR3071357.1"
                     /translation="MRHFFNVLSFAVALLPATLCAAQIQGKVIRVLDGDTIEVKTLPA
                     KIVVYEVPIRVRLINIDAPEKKQPFGRWSTNQLKALLAGQSVTVSYTQTDRYGRVLGR
                     VVTANGTEANRQQVLKGAAWVYDRYNTDNSLPALQREAQTQKRGLWADSNPVPPWEWR
                     HKQN"
     gene            complement(12581..12907)
                     /locus_tag="ON345_001271"
     CDS             complement(12581..12907)
                     /locus_tag="ON345_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071358.1"
                     /translation="MKIINVPDARSVRIAGIEIIDSVKSVASVFTEILLFMVWFPAIV
                     YGLYRFVIVLWDASLKPQSLIEFINFMVELYSYVLPSLAVIAVALVVVLSIFKRLSAI
                     WEGRKQ"
     gene            complement(12993..13307)
                     /locus_tag="ON345_001272"
     CDS             complement(12993..13307)
                     /locus_tag="ON345_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071359.1"
                     /translation="MKKTVTTAVLLCAFAAGTAHAEEKADPNDPCAMVLCLAGKLDGS
                     SPAECDPMYKSFMSIRKKNKHGFLPDHTADARKKKLNECPAADAGTVSKIISSFGRLK
                     NF"
     gene            complement(13395..13787)
                     /locus_tag="ON345_001273"
     CDS             complement(13395..13787)
                     /locus_tag="ON345_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3071360.1"
                     /translation="MKKMLMVSVLFLSACSSPPEPPQVDWEQNPETVNTQLMDWQPTY
                     SVIKSDKVNSSWVKVIHNFRPENRLYDDAVFYSVAHSDSVIVETSNGTDFFTAKNWLR
                     ANGANGVIQYRYKMNCFSCRTTSVYLSR"
     gene            complement(13784..15619)
                     /locus_tag="ON345_001274"
     CDS             complement(13784..15619)
                     /locus_tag="ON345_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3071361.1"
                     /translation="MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKN
                     FDSMLLWRIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKF
                     ASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPN
                     LLVRKHSLIALDPKQELWKITSKVREKLLGNKVYLLDPFNSKTHQFNPLFYIDLKEES
                     GAKDLLKLIEILFPSYGLTGAEAHFNNLAGQYWTGLAKLLHFFINYDPSWLGEFGLKP
                     VFSIGSVVDLYSNIDRELILSKREDLEGTKGLDENALYHLRDALTKIREYHETEDEQR
                     SSIDGSFRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDMSLAYDFLNL
                     FFNFVVEVTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIY
                     QNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKISEKLGYITTKSTGSSKTSG
                     RSTSKSSSESEAQRALVLPQELGTLDFKEEFIILKGENPVKAEKALYFLDPYFMDRLM
                     MVSPKLTELTASLNKTKKVLGVKGLKYPSKEKMLSVGELESEVLL"
     gene            complement(15622..16656)
                     /gene="virB11"
                     /locus_tag="ON345_001275"
     CDS             complement(15622..16656)
                     /gene="virB11"
                     /locus_tag="ON345_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HCR3071362.1"
                     /translation="MKDAENRHIVYEIIDEYFHHWLNEVDGLTEIAVNRPDELFVKVS
                     GKWQQHELKMDFKDCMSFAGAISDYHDGGSVTPEYPLRSVTLPGGERVQIVIPPATER
                     ETVSITIRKPSSVFIDHDTFVKQGFYSRLNQGGKFRDKEDELSSMFKDNCFEQFVPEC
                     LVKGKTMVFCAGTGAGKTTFANACLQYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPS
                     EGESSTVSSASLLRSGFRMNPDRILMTEVRGAEAWDFLKGSSSGHAGNLTTVHESTPE
                     DAVLGLVQRCYMNPECQNLPYNIILRRVLSNVDVIMSIKYIDEEDNRFASGIYYRDIH
                     FQEYFEKLKE"
     gene            complement(16653..16850)
                     /locus_tag="ON345_001276"
     CDS             complement(16653..16850)
                     /locus_tag="ON345_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3071363.1"
                     /translation="MITLDIKEFSMLLGIRESEIYHHIRKGIPINGVPFPKPLKQIKT
                     HRFNYEEVMRFIEDLKGKGEL"
     gene            complement(16852..18066)
                     /locus_tag="ON345_001277"
     CDS             complement(16852..18066)
                     /locus_tag="ON345_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HCR3071364.1"
                     /translation="MSEQENKIPTAVEIEQALRERHQKEIEQNDKTDKGDEDDGKSVK
                     RLGIEKLKKSRKGLVIIVAAFLLLAAGVSVYYIPSIIRAMSSGDDKPASNAVSTGSVK
                     RATGLSDDVDPFNTREEPAKTEERKESSGKAETPPEKVQQNFSRALDVAYGGSSSASS
                     NSGGSSSTASNTRNEGDGSDKQAEVQPVNAGQPAQLSKITRVPYDPNLFIPENTAIKC
                     SLDRRFISDLAGKLVCTINEDVYSANRNVKLIEKGTAAYLMYKTGTFNHGQGAVFIAA
                     TKLRTRKEPFIDIPLIDTQAAGALGEAGASGWIDTHFADRFMGAMMVGMIPDVAQAAS
                     GAAKSNKDNQTDYTANSRQAFADIAREAFANSVNIPPTLYKNQGEIITLIVGQDLDFS
                     SIYKLKMVGTRR"
     gene            complement(18063..18992)
                     /locus_tag="ON345_001278"
     CDS             complement(18063..18992)
                     /locus_tag="ON345_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HCR3071365.1"
                     /translation="MKMNKGALIVALLLVSHVCHAAVTPAASRFDPRNQIVSYNPQNT
                     TVINSAVGYTTTLVFDEDETVISARTGFPQGWEVNKEDNLVYLEVKPVKQTVQKNSTD
                     DNGNQSSEVVSVALDPENDLERWRTNLFVRTTKRNYSMELNARTFRQPDKIAFVVNYQ
                     YPQERRKEQAEIEKKRIEALNKRQEEQAINRSLENAKSPKNWDYWKRVADGSQSISPD
                     YAYDDGRYTWFGFSPLKKIPSVFVMSGEQETLTNPVVKNSGSFTVVGVPVDQRFVLRM
                     GNQVVGVENRGFGKVRLPAGDTVSPNVEKEVIQ"
     gene            complement(18998..19726)
                     /locus_tag="ON345_001279"
     CDS             complement(18998..19726)
                     /locus_tag="ON345_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCR3071366.1"
                     /translation="MGFKLSGLKLPGFNKKKEVTDSSTTFEEKNVLMQEKMNRIYKFG
                     GIAGIAVGVLSLLALNGALPLKSTVVEAYLINGVTGVAERLTSVKKENLSENEALAKY
                     FITQYVKRREGYNYFSLQHDYDYVLLYSAENVATDYNALINGNQSPKVIYNKAEKTAS
                     VQDNPSVIISPSSRRDDKDMGAYIRFKLTIRNVATGQDDYEYWNVRLTYRIEPQVEMA
                     SGDRNNNPLKFVVTSYVRDKEVKG"
     gene            complement(19921..20976)
                     /locus_tag="ON345_001280"
     CDS             complement(19921..20976)
                     /locus_tag="ON345_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCR3071367.1"
                     /translation="MATSRYFQAAHDVIVKTLDQSLTGQLDNFSSIASQLGKYGISIY
                     ILWYAYTTIVGKQKTAVQDFLWNLCRFWLILVFVKNMGGWLDSATQAIDGLKETFAGG
                     DPWKWVDELWEKVQQVAAYLMSKDTSKYVKTDGAIASLLTYAGGIIALLLTSIVYLAA
                     EVTIKILTITAPLFIICLSFGFLRQMFNSWLQLIFSSCFIFLFCGLAIKAGMTFLNGI
                     LTISIANADELNLISTGAQAGVAGAFMAWIIWQAKTYASQLAGVGVEGAMQGAAAMGI
                     GAGVFGASRMGRNLLGMGKNAGIGAWKGVRRQDGGFNQSPGVSGKLGNLTGQGVNIGA
                     KRLRQAAIDAAKKKYGG"
     gene            complement(20988..21245)
                     /locus_tag="ON345_001281"
     CDS             complement(20988..21245)
                     /locus_tag="ON345_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HCR3071368.1"
                     /translation="MKKYGLVAALGLTFFLTGCEEVKSVDWWQSHVDDATKKVAECKK
                     SGSDSENCKNAKEGLFRYKQLHPKHVDYKDAFKNVLGKDGS"
     gene            complement(21255..22025)
                     /locus_tag="ON345_001282"
     CDS             complement(21255..22025)
                     /locus_tag="ON345_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HCR3071369.1"
                     /translation="MKKHIVAALIASVTVISGAQAGVPVAIDANPEWAVEAQRWTERL
                     KQWQDTVNHYQKQINAYKQELLTKTGIRDVQGLVQSAQSVSKELENIYDQGNSFIDDY
                     IKNPEATLSEQARSLLADYKVTNTCKGLGYTGDLVRGCEASFLSQLAGIEYGNKLESK
                     LREDNQEMADLIDQVKNAQDTKATQDATNAVSLASLKFNKLKFQYEMYRDKQRDLAEY
                     KEKMLQAAFQEKQLGAVNKETPVVDYKAAFEQQSYEMN"
     gene            complement(22035..24788)
                     /locus_tag="ON345_001283"
     CDS             complement(22035..24788)
                     /locus_tag="ON345_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HCR3071370.1"
                     /translation="MSTVFKGLTRPALIRGLGVPLYPFLGMCVICVLLGVWIHEAMYA
                     LILPGWYAIKRVTKIDERFFDLLYLRMQIKGNPLANKRFNAVHYAGSSYDAVDISKVD
                     NFMKLKDQSSLEELIPYSSHITDNLIVTRNHDLLATWQIDGAYFECVDEADLALLTDQ
                     LNTLIRSFDGKPVTFYTHRIRVRKEVRPVFDSKIPFVNRVMNDYYESLSAAEYFENKL
                     YLTVCYKPFSAEDKVTHFLSRKKGNKNIFEEPINDMNEICGRLSTYLSRFHSRRLGLY
                     EENNIVYSEQLTLFQKLLSGRWQKVRVTNSPFYTYLGGKDLFFGNDAGQITASDHARY
                     FRCIEIKDYFQETDAGIFDALMYLPVEYVQTSSFTPIDKQSAIKALDDQIDKLEMTDD
                     AAKSLLADLKVGLDMVSSGYISFGKSHQTLIVYADSPERLVKDTNIVTTTLEDLGLIV
                     TYSTLSLGAAYFAQLPGNYTLRPRLSSISSLNFAEMESFHNFFTGKEKGNTWGNNLIT
                     LGGSGNDIYNLNYHMTTEHQNYFGKNPTLGHTEILGTSNVGKTVVMMTKAFAAQQFGT
                     PESFPPERKLKKLTTVFFDKDRAAEPGIRSMGGAYFRVKEGEPTGWNPAALPPTKRNI
                     SFMKDLVRLLCTLNSEPLDDYQNRLISDAVERLMQRSNRSYPISKLRPLILEPDDTET
                     RRHGLKARLNAWVQGGEFGWVFDNPDDTFDVDNLDVFGIDGTEFLDNKVLSSAASFYL
                     IYRVTMLADGRRLLIYMDEFWQWINNEAFRDFVYNKLKTARKLDMVLVVATQSPDELI
                     KSPIAAAVREQCATHIYLANPKAKRSEYVDELEVRELYFDKIKAIDPLSRQFLVVKNP
                     QRKGESDDFAAFARLDLGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTYLERA
                     NLI"
     gene            complement(24813..25103)
                     /locus_tag="ON345_001284"
     CDS             complement(24813..25103)
                     /locus_tag="ON345_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HCR3071371.1"
                     /translation="MLKLNKRYLTLSVFMAALMLCVAEPAFADDVSTKTTGFLQKIID
                     FLTDIRKPAITIIALVIGYIAIFSRQHAAWITPLIIGIIIFIVAPYLPDWLA"
     gene            complement(25087..25731)
                     /locus_tag="ON345_001285"
     CDS             complement(25087..25731)
                     /locus_tag="ON345_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR3071372.1"
                     /translation="MLSTTAFAALALQCAASVHPDTAHEVARVESGFNPYAIAEIIPK
                     VERKPGDKGVVSYFPKTKEAALQIVNQIESRNHRYSVGLMQITSTNFANFNTTAEKMF
                     DPCENLKVSEQILVDCYKRGGDILRGLSCYYSGNPETGTKPESDFNNTSYIQRIGFNP
                     PDNKKNWVVPSVKDAIRKENVTQSIKPKEVTVYPQYAMRGTVLDEKETNDVKTQ"
     gene            complement(25964..26473)
                     /locus_tag="ON345_001286"
     CDS             complement(25964..26473)
                     /locus_tag="ON345_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCR3071373.1"
                     /translation="MEWYVLQFTTTRFAAVFAHLERLNFSYFCPMETERYRRPDKIIS
                     YRERRLPLFPGYLFIQADFEEVHSTTITAIPYVQRFISFGGEPLPVPEDVMAELLYRQ
                     SHTTAQANLLRKSIPHDFAEILLMDNPQQRSMAFIHYITERSLTHKMKRKKNDCYPKK
                     NRNETQAPT"
BASE COUNT         6904 a         6537 c         5780 g         7417 t
ORIGIN      
        1 ttttccatta aagataatat taatattgct cagtataaaa catcatatgg tagcccatct
       61 tggcaagtaa acataaggct gccatttata aggcactgtt gcaaaaatgt ggaggtgata
      121 ggggtactac gccggaaccc cagatttttc cgtccgttca aattatccac ggttgaacag
      181 cttgatcccg tccgcccgca gttgaccaac aggggaaata tgcctgtaca aagtttgccg
      241 ggttatccca agttcctggc atagcgtact gacctttgtt tctgattgtc ccattgatgc
      301 cattgccagt cgcagtttga ctggcgtcat tttataaggt cggccgccgt tccgcccacg
      361 ggctcttgct gatgccagac ctgcagtcgt tctttcagca atcagttcgc gctcaaactc
      421 cgccagtgcg gcaaagatac cgaagacaag cttgccagcg gccgttgttg tgtcgatagt
      481 cgccccgtga ccggtcagca ctttcagacc ggtccccctc gcggtcaggt cgtgcactgt
      541 attaatgaga tgacgaagat cacgaccgag gcggtccagc ttccatacga ccagcgtgtc
      601 tccgggtcgt agtgctttca gacaggctgt aagtccagga cgctcttctc gtttgcccga
      661 tgcccggtcc tcataaaaat gtgcggcatc aacacctgcg gcgatcagtg catcacgttg
      721 taaatcattc gtctgcgaac catccgcttt tgacatccga atatatccga tgaacatatt
      781 tcctctgtca catatacgtt cgattatgtg acattctgat ttgggtacca taatctgtca
      841 acggtagtca cattacccgt aatttaaatt atgacacgta aaggttctat gttaatgata
      901 atgtgacaag cgcgtttaaa taggcataag gatcagagat gcctactaaa aataagctcc
      961 tcagtattct ttcggacgcc gagcaggaag cgctctatgg tctacctgat ttcgatgatg
     1021 cacagcggct ggagtttctg gcgctgaatg aatatgaact ggcgctggcc tgcagtcgcc
     1081 gggggcttca cgctcaaatt tattgcatca tccagatcgg agagcatgaa aaacctcggg
     1141 gcagccatcg aatgatgcca tctacagggg ttaaaataac caccatcatt tcataacaat
     1201 acctgctgac tatgaagatc tacgtcatca aaatagcagt tcatggagtc agtccgatgg
     1261 tctggcgtcg gctgagaatt gctgccgaca cgtcactggc cgcgcttcac ttcattttcc
     1321 agatagtgca gggctggggg gacgaccatc tccatcaatt tcacatttat ggcaaagatt
     1381 acggtatctc ctacgaagga ggcatcggtt ttgtcgataa tccgttccgg gtcgtgattg
     1441 atgattttgc ttttgatgct ggcgatcgct ttacctatga atacaatttc tttgagcact
     1501 ggcttcatga cattcgtgtt gaggccattt atgaaaactc tacgctgaaa gcgccgtttt
     1561 gtataagcgg ccatggtatg cccggagcca cagctgcgga tgagttcgat aaaaccctgg
     1621 cgtttcttga agctatcgtt aatgcggatg atgaaacaac ggtcggcgag atccgccctt
     1681 ttgctgacga cctggatgct gtcaggttca accgccacaa aatcaaccgg caactgagca
     1741 ggctcgacct tgcatctccg gtactggagc ctgaagttat ctggctgggc cgtcgtcgct
     1801 gactgatgtc tataaaggta tacgttttca gcatgcctct cagatccccg aaaaaatgtc
     1861 tataaacctc cctaaatcag tgtttatgga ttattcttta aatctctatg atgtttaaag
     1921 accttccgga ggttttgcgt gcaatatgcc tacatcaggg taagctcggc cgatcagaat
     1981 acggcccgtc aggaagaggc attgtcaaag gcgggttttc agcctgataa aatttgtgtt
     2041 gagcatgcca gcgcgaagga cacaaaccgc ccggggttac aggaactcct ggggcaatta
     2101 cgccctggtg atacgcttct ggttcattcc atcgatcgcc tttgccggaa tatgtcggat
     2161 atgtgcgctg tcaccacccg gcttcgtgac cagggcgtta cccttatttt tctgaaagag
     2221 cagctgacgt tcagcgccgg cacaaacaat ccgatgcagg aactgcagct gcacatgatg
     2281 tcggcattca gccagtttga acgagcctta ctgaaggaaa ggcaggccga cggcatcgcc
     2341 gcgaaaaaag cacggggtga aaaaacaggg cgccccggcg ccgatatcaa aaagatccag
     2401 gaaattgacg cactcagaag cagaggggtc aggctcagga tcgcctgcga ccatgcgggc
     2461 cttggcgttt ccacatatta taagcttcgt caccagatga acaaccagta gccgggagat
     2521 agtcatccac cacagcagga ggcaatgatg caactgacgc tccagatagt catcaccgat
     2581 gaatcaggct ccagtcgaac ggaggaactg atgacaatcc agaaatcagg ggagacccgg
     2641 aacgacatcg gattatcggt gtcagaatcc aagctgttgc tgaatacggt ccagcagtcg
     2701 gtggtccagc tgcaggcaga cgaatatact caacaccata tccggtgccc tcactgcctt
     2761 gctgcgcgca gaatcaaagg caaacagaaa atacggtacc ggacgctgtt tggcgttatt
     2821 ccggtatccg ggcttcgggt gtaccggtgc cgttgtgaag agagtgatac aaagacagtc
     2881 agtttgctca gcgactgggc cggcgattat tctcacccgg cactgaaata tattgaaacc
     2941 cgctgggcct cgatgatctc ctatgaaatg acgactcgtc tgctgaaaga tattctgccg
     3001 gtgggccaca gcctgaatgc ctcaacggtg aggaatcatt tatgccaggt ggcgcagcgt
     3061 cttgacgctg aggctgaagc tcattcaggt tttctttccg gctgcccccg cgactggggg
     3121 aacctgccca ggccaggaaa accgcttgtg gtgggtatag atggtggtta tgttcgcgat
     3181 cgggatgaca aaaagcgtaa ttttgaaatt attgccggga agtctttttc cgttggtgcg
     3241 cctgctgaca cccgccgctt cggttttgtt cagaaggatg actgtcatcc ggaacgcagg
     3301 ctcatgaccc acctttcagc gcagggaatg caggccaacc agcagatatt ttttctgtcc
     3361 gacggcgcgg ataatctcag ggacctccag ttcggtatgt accctgagtc aacgcatgtg
     3421 ctggactggt ttcatatcac catgaggctg acggtgctca tgcaatatgc ccggggactg
     3481 ctggtatcag atccagaggc ggggagtaaa gttctggcac tgctggaaag cattaaacga
     3541 tatctctggc atggcaatgt tgtcgctgcg ctggaacata ttgataattg cgttatgtat
     3601 tgcgatgacc ctgaactcag ctatcctagc ctgaaatccc tgcaaaaaca tctggatgaa
     3661 atgtatacct acatccggaa taataagatg atgatcccaa actacggcga aatgcgtcgg
     3721 tacggagagc cggtatcaac cgcgtttgtg gaatccacga tcaatgaagt gatcgccaga
     3781 cgaatggcca aaaagcagca gatgcagtgg agcagaaaag gggctcatta tttgttacag
     3841 acccggaccg ccgttttgaa taacgaactg caggataaat ttgtctgctg gtatccgggt
     3901 tttcaaagtg acgggaaggg accagcgatg gcagcatagc tcacgctgcc ccaatatttt
     3961 ttatgctctc ctgttaccga tgcaagaaca ggggagtttc atgaaatgcc tgtttaggag
     4021 tgattaaagg taacttgtgc gcagaaggtc aggtgtgtgg tggttagtac agactagtga
     4081 atgttgtaac taaactatat ttttattaaa ggccggaggt ggtgttcctc cggccttaat
     4141 tacgtttccg tgttcattag tcgatgaact ggcggatcag ttccagaatg gcaatcagcg
     4201 cgtttgcaag cgccgctacc gccctcagaa cgatgattaa caggttaatc atccgttttc
     4261 tccataaatg gagtaaagac accgtggctt gctcttacac tgcctgtcac cacggcttct
     4321 cttcgtcttg tcagttgtag agagtctcac tcctgccaga gcacagataa ccagcaccac
     4381 ctgtattacc tgtcaggact ttgaacctgt tcatgccgca tttagcggaa tgcagggaga
     4441 gactcaccac agagctaacc ctctgattat acgtttatct aacgttcagg caaaccgtta
     4501 acgcacagtc actgcccgtc atattgcata atcaggcttc acggattatc atactattgt
     4561 cagttgtaga gagtttccct tccgggctga gttaccagct cgcaccggaa ccgtaaaaaa
     4621 gccgacctca ccagtcggtt tttttacgtc tgcggtttcc cgcttcgttt aaatctattt
     4681 taaatgaata aattgttctg cggcaaaccc aacaacataa ggacgttagg gttgatggtt
     4741 gccgaaatcg cggtgcgtta cgtgaattct gaggttttgc gagggggatt gggcgcgaga
     4801 atttacgaga gagcgccgga acaatgccgg gatcacaaac catcacccgt gaaagaaatc
     4861 ttccggcatt gcccggtcag gtagtatgac attcattgtc aacggccatc aatttataag
     4921 ctgctttgca cttcataaaa gatcaccacc agggctgcta aaacagtacc gatagccagc
     4981 acgttaaaaa gtacgtcctt catctttacg aacgacatac tgatttgcgt cctttccctt
     5041 ttgtcagcct tctctatcat ccagcgttgg tatgaatcac tgttactttt tctgtccatg
     5101 ttccccccaa ttcatcaatg agcaccaggc ggccaccagg cttcttgcca acaaagtaac
     5161 ggcctcgcgg cagacgttcg ccagtcttca gccgttcgat ccagtcctga aatgcagggc
     5221 gcggtttata gctgtagcgg ccaaaatcag ctaaagactg cgtgaccatg actgtttggc
     5281 agccatgctt tctcatatca cgaaacgtat tttccagtgc agccagtgta ttcatttgca
     5341 cgttctctct gcttttccca tgaagatatt aacgacactc aaaactaaca ccgttggcat
     5401 gatcacggca gcatagccga cagcaacggc ctgatatatg gtcaatgagt agtcgggctg
     5461 tttatacaat acgactgaaa aaatgctccc caaacaagca agcagagtta aaaacaagta
     5521 atcgacaaat ctgtttttat agataaccca tgcaacgatg cagggatatg acaccaggca
     5581 caggataatc gcatttgtgg tatgcacgtt tcgctccttg ttatttaagg gttgtcagcc
     5641 agatcaaaca gattgttcag gctggaacgg aattgtttta ccgatgatac cagctcctga
     5701 ctccgcgctt tttcctgcgc cagttcacgc cgtagctgcg ccatctcgtc tctgttacgc
     5761 tgaagctcaa ggatagccgt cataaaggtt ttgctggctg atgcctgtcc ggtcgcttcc
     5821 tgcgcctttt taatggcctg atcttcgtcg tcattcgttc tgattgtgta atgccccatg
     5881 atacccctct tttgctgtca tttttgcgct gccggcgcga acccgggaag aaactttgct
     5941 ggcaaaaaac actggccaac agatgtgacc gggaaaattt gctggcaatt aattttcgat
     6001 gaaagccagc tcgcaatcgc cgcattcaat attcagtccg gctttgcccc acacggcagc
     6061 gccacacccg cgacactgat atttaacttt atttcgtttt ggcttgtcct caaatgctgc
     6121 aactgacaaa tcattgtcat tgctcccggc catatcatct gaatggctcc cgttctgcgg
     6181 tgacactgac ggcagcagag ccatagaaag caccgcttct atatcaatcc cggcctcagc
     6241 attttgatgc gccagagcaa gcgtttcgcg ccattgctct attacggcgg tcatatcttt
     6301 ttgttgttcg acctggacag ggaagcgatc aaaccagctc aaagcaaatt gcccctggaa
     6361 cagttccagt gtgacgcgct ggaagcggcc accagggatc ggatagtcat taatcttctg
     6421 gccgactttc gcaccaccag gtctcccggt ggaggagggc ataagtccga tactttccat
     6481 ttttgccgcc cactgcgcat tgtggtacgt cttacgcgac gggttgccgt agtggtactg
     6541 ccacatatgg cactgctcat gaaccagcgt ttgcatgact tcaataagcg ggtacaccgg
     6601 gaagtattca ggattgaggg cgatttcatc aatcatcctg ccgctgccgt cagcagcaac
     6661 aaagcgcctg taactgaaat agcccattgt gttcttgccg cgctggaacg taatcagaca
     6721 gtcagggagt tcgccgtcaa aaaggcgctg gttatagaaa tcaaacgcct gttgtagctc
     6781 gctgtaagcg cgttccgtgg ctcttgtcat gctgaagttc gcttagcggc gttccgatat
     6841 tcctgtaact ctgcaacaat agcagggaac acatcagcgc aacgctgtgc cagcttaacc
     6901 gcctttgaac aaacgcccat accatgtgct cttttgagcg cctccaacaa atctgccagt
     6961 tcctcgtcag tgattttttc agtcatctat taagcttctc cattatctgt atcgtacgat
     7021 acagataata taaaccacac ctttgatagc ggcaatcttt tttgctggca atttcacgcc
     7081 cagggcgggc atcgagcggg taatattgct ggcaaaaatc aacggccaaa ccggctgcag
     7141 gaaactggtt tgctggcaag cgtgatttac tgctgggctt gacaccttgc cattgtatga
     7201 cgcttgtttt agtgtcaaat gaacaaccag cgcgagaaga atataggaag caaaagcaat
     7261 ggatatcaaa cctaactcag tgatccacct ggagccaggc gaaacattac ccggctatac
     7321 caatctgaaa cccgtacctg ataagtattt cgatgagtta aaggcactca taaataaact
     7381 caatactcaa tccggtaata tttacctcaa gttaaaacag atgtacgcct ttttggatcg
     7441 ttttaacaaa gaatttgtga gcacgtttac ctcctgtcaa aaggggtgct cttcgtgctg
     7501 taaaatggat gttcatttaa ccgcgcttga ggcaacacat attgcacaag caagtaaact
     7561 tactgccaga gacaacccgc ttacgaccgg gcatgagtca aaatgtccgt ttttgtctga
     7621 gaagggaaca tgctcaatct acaattaccg cccgctgtta tgcaggacat atcatgtact
     7681 cactccgccg gagatgtgta atgacctgga tgcacaagtc atgcagtatg gttctcaaag
     7741 cgcgaacatg ggaaatcaca tctacaaaac aatagcagag tggatctatt ttcagacgta
     7801 tcactgcact ggtaagttag aaaccaaaga tatcagagac tacttccctt atccccgcga
     7861 agatatccaa agatttcttc accataaccc cccgcgccct ttttgctaaa aaaaagcctc
     7921 tgacgcagtg tcagaggctt gttagattac tgttcgtcac cagcagtgct ggaagcatct
     7981 tttttgatga gaaactcatc aagagaacgg ccagctttta acgcttcgtc gattggtttt
     8041 ggtgtccggc cacgaccaga ccagtatttc gtttcgccgt tttcatcaaa ctgatattta
     8101 ggcggcgctt ttggcaacgt ctttttgcgt gttttttgcg catcttcgga caggccaagc
     8161 agttcttccg gtgaaaaacc ttctccggca atcaactgga gcagttcctg gcgctttctt
     8221 tcgcgctctg cgcgttctgc tgcctcacgc tctgcttctt cacgacgttc ttcgatcacg
     8281 gcgttaagct tttcctgcat ttccagcagt tgttcaaagc tggtttctcg cgcaaaaaca
     8341 cgaacagagc gaatattcag gagtagcttt tttgttgcgt caaacgtttc attttcactc
     8401 atcgagttaa tccttatttt ttactgaact gaaacccaag tttacccgta gttttatcct
     8461 gcaaaacaat tgcagcgtca aactttttcc cggttttatt actggtaaag cctttaatct
     8521 ctccggtttt accttttttg attaatgtct caacctgatt ttgtgtcagt ttttttcctg
     8581 acacttcaga ccagatttta aactcgcacc cggaacagaa aaagccttta ggccggataa
     8641 taatttcttt actgcatgaa gggcaggggg cttcgagccg ggacatggaa ccttctgatc
     8701 tggattttgc ttccatatcc ggtttgccgt ctttgtccgg gaatgttgtc ttgcattcag
     8761 ggtagcagga gcatccccag aaaaagccat tttgcccctt acgcttacgc aggaaaccgt
     8821 tgttacaaac cgggcagggc gtggcatttg aagaaatcga aattccgttc ctgtccagct
     8881 cgtcgattaa cccctggacg tactcatcat tttccttaat aaactcctca actgtcagct
     8941 ctccggcttt gatctgagac tgcttttcag cccacagagc ggagatatcc ggtttagtga
     9001 tttcaggagg cagtgcagcg caaaactcct ggccttgttt tgtggttttc cataccagct
     9061 ccgaataccc cttttctttt tcgatggaga taagcccggt atttgccgcc agtttctcaa
     9121 gaatacccgc gcgggtggct tccgttccga tactgccacg atcactgctg ccctcgtctt
     9181 tcgcttccag cgcctttctg agagccggat catcaatgaa ttttgccgca ctcgtcatgg
     9241 cggccagtaa cgttgactct gtgaaatagc gaggtggtgt agtctttttc ttgtccacgt
     9301 ctgccgattc acacaggccg ctgtcattaa atttcagcaa tgaaaggtca aaaccgtccg
     9361 ttccggcatc ttcggtttcg tcctcgtcat cactatcctt acccagcgct tcccagccct
     9421 tttgtaccag aacgctctgt gtggccgtaa aggtatcccc cttaatatca aagtggattt
     9481 tcgtcttgtt gcggatcgca tccgggtaga acagcccgat aaagtagacg gacaccaggt
     9541 tataaacgtt ccgctctttt tcattaagct gaataccggc tccgcttttt gtcgtcggga
     9601 tgatggcgtg gtgcgcctca atcttactgg cattaaaggc tttgtgtttc tggcctttat
     9661 ccatatctac gatcgcagac gccagttcgg gtacggtggc cgctatagca gtggcaatat
     9721 ctccggcctg gtaataatgc tcatctgaaa gatagcgatt gtcagaacgt ggataggtca
     9781 gaagcttgtg agtttcatac agcccctgca tcacatcgag cgtatctttt gcagaataac
     9841 caaatcgctt tgcgcagagc tgctgaagcg tggaaaggtt aagcggaagc ggaggcttgc
     9901 tattttccgg ctttgtcgtc gcgatagtaa caatagcatc cttacccgta acacgtccgg
     9961 caatatgcgc ggcctgagct tcggagatca ggcgtttttt ctcgtctatc tgatcgtcgt
    10021 ctgttgtctg atatttcgcc ttaatctgat gcccgttcat cgacagactg gcgaatacat
    10081 cgtaataaaa gctttctgtg tgattctggt tcgccagcgt gcgcatgttc accagaccaa
    10141 gaacggcact ttgtacccgg ccaacgttca gaacgccgtc atagcccttc tcacggcctt
    10201 ttaatgtaca tccccgcgtc atgttatagc caaaaccctg atcgcacagg gaacgcgcca
    10261 gggcgcttaa cgtcataccg cgaaatttat cgttaggttg taaattcgac agtgattttt
    10321 gcactggcgc aaggttgagg tcggcaacaa gcagtcgctg gacgggtttc ttgtttccgg
    10381 cataacccag aatctcatca accagcaaac atccttcatc atcggggtcg cctgaatgaa
    10441 cgatgctgtc ggctttatcg ataagagaca aaataatgcg ggtctgtgct tctgattcag
    10501 gcttgatttt atattttggc ggataaaccg ctttaatggg taaatcacta taacgccagt
    10561 tggaatattt gtcgtcataa tcctgtgggt cgtaaagctc cagcatatga ccataacacc
    10621 aggtaacgac atcagaaccg tgttcaaaat agccgttttt cttttctgta ttgggatttc
    10681 cgcccagtcc ttcaaaaata gcctttgcta aagacggttt ttctgcaata aaaagtctca
    10741 ttaatcaatc ctcaaggtaa cttgaatagt caggctgcgc accattcttt ttaaagattt
    10801 cttccctttc ctctatgtct tcttctgcct cctgcacact aaggtcaagg gttcctttca
    10861 aaaaggcacg cgcattcttg attacatcta ctgttaattc gttttttgtt ccgcgaatca
    10921 tgccaatagc aatatttatt atctccttta tttcagcagg agtcgttctg tccagcaccg
    10981 cgccaagttc tttaaggtca atatttatat cgtctggtaa gtacattttg gcacaagcgg
    11041 cagcggcttt agtccgaaaa gcacctacag atatgattct gtcaattctg ccagggcgtt
    11101 ttctgatgcg cggatcaatc cggttaggat agttcgttga aaaaataaca tatgtcccgg
    11161 caagatttcg cggtgtatca gccccatcaa gaaaacttaa cgttccggca tcagcccgat
    11221 aaagttcatc aacctcttca gctattatga taacgggtat tttcttttga gctgcggcat
    11281 agcaaacatc tttaaagtaa tcatcagcat aaacaaatac atatttatcc tgatattttg
    11341 cccccaaagc ttttgcgata gtcgttttcc ctgtgcccgg aggaccagta aaaagcactt
    11401 ttcgcattcc tgactgacca taacgggtgt agaaatcaat atccgaaaag aactgattaa
    11461 agtcattctc cagctcatta aactttggat gaacagaaaa acgtaaagcg ttttctattt
    11521 cttccttcct tgccttaccg tgataataaa cgccgtcgta acacccgatt ttccagatac
    11581 ctttacgggg taaagagcgg tttaatttag cgcgtccagc ttcgcaaaga ttatgaagca
    11641 tcgacatact tttggttgtg ccaactgcgt actccattct gactttagag ttaagaatga
    11701 agcgaataat atgaaccact tcattttgac caagatgaat ccggtatgct cccgaaccat
    11761 ctttaaacat aagcttttta atcctcccgg ctttagccat aacagggcta cttcccgtat
    11821 gaacgacggt tgaagcaacg ggttcgatat caagattcga ataaatttta cgattctttc
    11881 tgtttataaa aatctctgcc actttaggat agtaatcaca atcctttacg acagtaccaa
    11941 aattatcttc accgtaatat tctttatgga gccgtaaagc ctgctctacc agagttaatt
    12001 tacccatatg cgcaccatgt atattaaaaa ccgtgacgtt gcctgaagat tttcttcagg
    12061 cagaggaggg gttggacaag cgaagcgcgt cagttttgtt tatgacgcca ctcccacgga
    12121 ggaacgggat tactgtcagc ccacaggccg cgcttctgtg tctgagcttc ccgttgcaga
    12181 gccggtaatg agttatcggt gttgtacctg tcataaaccc atgcagcccc ttttagcact
    12241 tgctggcggt tagcctcagt gccgttcgct gtcaccactc gtcccaggac tcgcccgtag
    12301 cgatctgttt gcgtataaga gacggtaact gattgccctg ccagcagggc tttaagttga
    12361 ttggttgacc agcgaccaaa gggttgtttt ttttccggtg catcgatatt tatcaatcgg
    12421 actcgaatcg gaacttcata cacaacaatt ttcgcgggta gtgttttaac ttcgatggta
    12481 tctccatcga gaacgcgaat aactttcccc tgaatctgag cagcacacaa tgtagctggc
    12541 aacagggcaa cagcaaagga aagaacatta aaaaaatgtc tcattgcttt cttccctccc
    12601 atattgctga gagtctttta aaaatggata aaacgacgac taaagccact gcgataacag
    12661 ccaacgaagg aagtacatag ctgtataact caaccatgaa attaataaac tcaatgagcg
    12721 attgcggctt taaggaagca tcccacaaaa cgataacaaa acgataaagc ccatagacaa
    12781 ttgccggaaa ccagaccata aacaacaaaa tttcagtaaa aacagaagcg accgatttga
    12841 cactatcgat aatttcaatt ccggcaattc tcacacttct tgcatcaggt acgttaataa
    12901 ttttcatcct cccacccctg taaaaaatgt tcgcaagttc cagtctcaaa cctgaaactt
    12961 gcgtattcac atcagaatta aatcaaacag aattaaaagt ttttcaatcg gccaaagctt
    13021 gaaataattt tacttactgt acctgcatca gccgcaggac attcatttag ttttttcttt
    13081 ctggcatctg ccgtgtgatc gggtaaaaaa ccatgtttat tctttttcct gattgacata
    13141 aaacttttat acatcgggtc acattctgcc ggactacttc cgtcaagttt tccggcaagg
    13201 cataacacca ttgcacatgg atcgttcggg tcggcctttt cctcagcatg agcagttccg
    13261 gcagcgaacg cacaaagcag cactgccgtg gttacggtct ttttcatata cacctcatgg
    13321 ataacgggca actaacgccc tgcggttgtt caaccccggc tcaataaaat tacaagaatt
    13381 ttattaatcc ggcgttatcg tgataaataa acactggtag tccggcaaga aaaacagttc
    13441 attttataac gatactgtat gacaccgttt gcaccgttcg ccctcaacca gtttttagct
    13501 gtgaaaaagt ctgttccgtt actggtttca acaatcactg aatcagaatg tgcaacagaa
    13561 taaaaaacag catcatcgta aaggcggttt tcaggcctga aattatggat aaccttaacc
    13621 cacgatgaat taaccttgtc tgatttaata actgagtatg tcggttgcca gtccattaat
    13681 tgtgtattca ccgtttcagg attctgttcc cagtcaacct gtggtggttc aggcggagag
    13741 gaacaggcag agagaaaaag gacactgacc attaacattt ttttcatagt aaaacctctg
    13801 actccagctc accgacggag agcatttttt ctttagaagg atatttaagc cctttaacac
    13861 ccaatacttt tttagtctta ttcagagagg cggttaactc ggtaagtttg gggcttacca
    13921 tcatcagcct gtccataaaa tacgggtcga ggaagtagag ggctttctca gctttaaccg
    13981 ggttttcacc tttaagaatg ataaattctt ctttaaaatc cagcgttccc agctcctgcg
    14041 ggagcacaag ggcgcgctgc gcctcacttt ctgaactgct tttcgacgtt gagcgtccgc
    14101 ttgtcttgct tgaacctgtc gatttggtgg tgatatagcc cagcttttcc gaaatctttt
    14161 tagcgtcatc ttcttcactg acagcgtaga taatccggca gggatgcgca ctcatcagcg
    14221 ttttggcacc ttcaacacca tagatttcat taagctgact gatattctgg taaatcgtca
    14281 gaagtttgag tttaaacccg gcaatataac ccgaaccttt tttaatgatt ggcatgtaac
    14341 caatagacgg aaactcatcc aggaacatca gacagtcatg tttcagtgtc gggtcaaagt
    14401 cagggttttc tcgtaacgtg acttccacaa cgaagttaaa gaacaggttg aggaagtcat
    14461 aggccaggga catatcttca gcattaacgc caacataaac agtgatatct tcccggcgca
    14521 actggcgaag gtcgaaatca ttgccgtctg tacatttgcg aacagtcgga agataaaaca
    14581 gactcatttt cttacgaaac gaaccatcaa tactcgaacg ttgttcgtct tcggtttcgt
    14641 gatactccct gattttggtc agggcatcgc gcagatgata cagcgcgttt tcatcaagtc
    14701 cttttgttcc ctccaggtct tctcgcttac tgagtatcag ctcccggtcg atgttgctgt
    14761 acaaatcgac tacagaacca attgagaaaa caggtttaag cccgaactca ccaagccagg
    14821 aggggtcgta gttaataaag aaatgaagca attttgccag ccccgtccag tactgccccg
    14881 cgaggttatt aaagtgtgct tccgctccgg tcagaccgta agacgggaac aggatctcaa
    14941 tcagcttcag caggtcttta gccccgctct cttcttttaa atcaatatag aaaagcgggt
    15001 taaactgatg cgttttactg ttgaaagggt caagaagata gaccttatta cccagcagtt
    15061 tttcacgtac cttactggtg attttccaca gttcctgttt tggatctaat gcaatcaggg
    15121 aatgtttcct gaccagcagg tttggaatac ctattgcagc ccctttacct gcgcgggttc
    15181 ctgcgccaag tgatacaaaa tctggtgcgg tataccacag gtatttacct ttatatgcac
    15241 ctacaagaat atcagtatcg ttttctttct cccatttcag aagtttcgat tttcttaaat
    15301 cattatcact ggcaaacttc gcgtcaccat aaagagcaac atccgtttta ttcagttgcc
    15361 agataataaa aacaggcaca atgagagacg aaaccatacc tgataaaaga gaggggattg
    15421 cggttaacct gatatctgtc cgtatattac tctctgttat tatccgccac agcagcatgg
    15481 aatcaaaatt cttccatata taaagcggcg tttttccgtt caggaagtaa acagcaactg
    15541 agccagtata atacgtcaca agacacataa ccagaccgat aaaagcccac tggcctttat
    15601 cgggtagttt cagagacata atcactcctt cagcttttca aagtattcct gaaaatgaat
    15661 atcacgatag taaatacccg atgcaaatcg gttatcttcc tcatcgatgt acttaatact
    15721 cataatcaca tcgacgttgc ttaacaccct ccgcaatata atgttataag gcaaattctg
    15781 acattcaggg ttcatatagc accgttgaac cagtccaagc acagcatctt caggggtgct
    15841 ctcatggacg gttgtaaggt tccctgcatg gccggaactt gagcctttga gaaaatccca
    15901 ggcttccgcg ccgcgaacct cagtcatgag gattctgtcc gggttcatac gaaaaccaga
    15961 acgtaaaaga gaagcggaac tgacagtgct actttcccct tctgacgggt aatacaattt
    16021 cacatgattt ttgtggaacc tgaactttgc ttcatctgta tcttcaattg aaatacatcg
    16081 taaatgatgg ggtatgtact gaagacaggc atttgcaaag gtggttttac ccgcccccgt
    16141 tccggcacaa aataccatcg ttttaccctt aaccaggcat tcagggacaa attgttcaaa
    16201 acaattatct ttaaacattg aagataattc atcttcttta tccctgaatt tccccccttg
    16261 atttagcctt gagtaaaacc cttgtttaac aaaggtatca tggtcgataa acacactgga
    16321 cggcttacgg attgtaatag acaccgtttc ccgttccgtt gccgggggaa taactatttg
    16381 tacacgctcc cctccgggga gcgtaacaga acgtaaggga tattctggcg taaccgaccc
    16441 accatcatga taatcagaaa ttgcccctgc aaatgacata caatctttaa aatccatttt
    16501 taattcatgc tgctgccatt tcccactcac tttaacaaac agttcatcag gccgatttac
    16561 cgcgatttca gttaaaccat cgacttcatt aagccagtgg tggaagtatt catctattat
    16621 ttcataaaca atatgtctgt tctctgcatc cttcataatt caccttttcc tttaagatct
    16681 tcaataaaac gcatgacttc ttcgtaatta aatcgatgcg ttttgatttg tttcagtggt
    16741 ttggggaaag gaacgccatt tattggtatg cccttacgaa tatgatggta tatttcgctt
    16801 tcccttattc ctaacagcat tgaaaactct ttgatatcca gcgttatcaa attacctccg
    16861 ggtacccacc atttttaatt tgtagatgct ggagaaatcc aaatcctgac caacgatcag
    16921 agtaatgatt tccccctggt ttttataaag cgttggaggg atatttacgc tgtttgcaaa
    16981 cgcttcgcgt gctatatcag caaaagcctg gcgactgtta gctgtatagt cggtctgatt
    17041 gtctttatta cttttagccg ctccacttgc ggcctgtgcc acatcgggga tcatgccaac
    17101 catcatcgcg cccataaagc gatctgcaaa atgtgtgtca atccagcctg aagccccggc
    17161 ctcacccagc gctcccgccg cctgtgtatc aatcagcgga atatcaatga aaggttcctt
    17221 acgtgttcta agcttcgtag cagcgataaa cacggctccc tgaccatgat tgaacgtgcc
    17281 cgttttatac atcagatatg cggctgttcc cttttcaatt agctttacat tcctgtttgc
    17341 actgtatacg tcctcattaa tcgtacagac cagcttgcca gccagatcag aaataaatcg
    17401 tctgtccagc gagcacttaa tcgcggtatt ttcaggaatg aaaaggttag ggtcataagg
    17461 aacgcgcgta attttagaaa gctgcgcggg ttgccctgca ttaacaggct gaacctctgc
    17521 ctgtttgtca ctgccgtccc cctcgttcct ggtattgctg gccgttgatg agctaccacc
    17581 ggaatttgag gaagcagagc tgctcccccc ataggccaca tccagcgcac gactgaagtt
    17641 ttgttgtact ttttccggtg gcgtttccgc tttgccgctg ctttccttcc gttcttccgt
    17701 cttagccggt tcttccctgg tgttgaatgg atcaacatca tcgctgagtc ctgtcgcgcg
    17761 ttttactgaa ccagtagata ccgcattact ggcaggctta tcatcaccgc tggacattgc
    17821 acgaataatc gacgggatat agtagacaga caccccggcg gccagcagaa ggaaagcggc
    17881 cacaataatc accagcccct tacgcgattt tttaagcttc tcgataccca ggcgttttac
    17941 tgatttaccg tcatcttcat ctcccttatc cgttttatcg ttttgctcaa tttctttctg
    18001 atgccgttcc cgtagtgcct gttcaatttc tacagcggtc gggattttat tttcctgttc
    18061 gctcactgga tcacctcttt ctcaacgttc ggggaaacgg tatctcctgc cggaagacgg
    18121 actttgccaa agccccggtt ttcaacccca acaacctgat ttcccatacg caaaacaaag
    18181 cgctggtcta caggaacacc aacaacggtg aaactaccgc tgtttttgac taccgggtta
    18241 gtcagcgttt cctgttcgcc gctcataaca aagacgctgg ggattttctt cagggggctg
    18301 aagccaaacc aggtatagcg gccatcgtca tacgcataat ccgggctgat tgactggctg
    18361 ccgtcagcta cccgcttcca gtaatcccag ttcttgggcg acttcgcatt ttcaagagaa
    18421 cggttgattg cctgctcctc ctgccgcttg ttaagagcct caatccgttt cttctcgatt
    18481 tcggcttgct ccttccggcg ttcctgcggg tactggtaat taacaacaaa cgcgatctta
    18541 tccggctgcc ggaacgttcg ggcgttcagc tccatgctgt aattacgttt tgttgtgcga
    18601 acaaacaggt ttgttcgcca gcgttccagg tcgttttcag gatcaagggc tacactgacc
    18661 acctctgaag actggttgcc attgtcatcg gtactgttct tctgtacggt ctgtttgacg
    18721 ggcttcactt cgagatacac caggttatct tctttgttca cttcccagcc ctggggaaag
    18781 ccggttctgg cactgatgac cgtttcatcc tcgtcaaata ccagtgtggt tgtatagcca
    18841 accgcactgt ttatgactgt cgtgttttgc gggttataac tgacaatctg attacgggga
    18901 tcaaaacggc tggctgctgg cgtaacggct gcatggcaga cgtgcgacac caacagaagc
    18961 gccacaatta gcgctccttt attcattttc atagttttca ccctttcact tctttatcgc
    19021 gtacatagct cgttacaacg aatttaagcg ggttattgtt tcggtcaccc gatgccattt
    19081 cgacctgtgg ttcaatacgg taggtcaacc ggacgttcca gtattcataa tcatcctggc
    19141 ctgtcgcgac atttcgaatg gttaatttaa accggatata tgctcccata tccttatcat
    19201 cgcgccggga tgacggactg ataatcacgg acggattatc ctgaacagat gcggtttttt
    19261 ccgctttatt ataaataacc tttggtgact ggttgccgtt gattaaagcg ttgtaatccg
    19321 tcgccacatt ctcagcgctg tataacagaa cataatcata atcatgctgg agactgaaat
    19381 agttataccc ttcacgacgc ttcacatact gagtaatgaa gtatttcgcc agcgcttcat
    19441 tttcagagag gttttctttt ttaacagaag tcagtctttc agcaacgcca gtgacaccat
    19501 taataagata agcttcgaca accgtactct ttaacggtag cgctccattc agtgccagca
    19561 gagacaacac acctacggca attcctgcaa taccaccgaa tttataaatg cggttcattt
    19621 tctcctgcat caatacattt ttctcttcaa aagtagttga agagtctgtt acttcttttt
    19681 tcttgttaaa accgggaagt tttaatcctg agagtttaaa ccccattgcg cattacctct
    19741 gctgaattaa caggttcgct ttttccggat accggaggtt gtttatgtga cgcctggcat
    19801 ccgacaaggc aaagcgtcac gatagccgtg ataagtagtt tcataatgtg agaacctaaa
    19861 tagaggagtg aatatttact gcggaatact cagagttgac agatttaaat aataaatcgg
    19921 ttaacctcca tatttctttt tcgccgcatc tatcgcagcc tggcgaagcc tcttagcgcc
    19981 aatattaacg ccctgcccgg tcagattccc caacttaccg gatacaccag gagactggtt
    20041 gaaccctcca tcctgacggc gaacgccttt ccatgcgcca atccctgcat ttttacccat
    20101 accaagcaga ttacgcccca tacgggaagc gccgaacacg ccagcaccaa tccccattgc
    20161 ggctgcccct tgcatcgcgc cttcaacacc tacccctgcg agctgggaag cataggtttt
    20221 ggcttgccag ataatccagg ccataaatgc acctgcaacc ccggcctgag cacctgttga
    20281 tatcagattt aattcatctg cgttagctat cgagattgtc agaatgccat taaggaacgt
    20341 cattcccgct ttaattgcca gtccacaaaa caagaatatg aagcatgagc taaatattaa
    20401 ctgaagccag ctattaaaca tctgccttaa aaaaccaaat gaaagacaaa taataaacaa
    20461 cggcgcggta atagtaagta tcttaatagt aacttcggcg gcgagataaa ctattgaagt
    20521 tagcaataat gcaattatcc ctccagcata tgttaataaa gatgcgattg ccccatctgt
    20581 tttcacatac ttcgaagtat ctttggacat aagatatgca gctacctgct gaactttctc
    20641 ccaaagctcg tcaacccatt tccaaggatc acctcctgca aatgtttcct tcagcccatc
    20701 aatggcctga gttgcactat ctaaccaccc ccccatattt ttgacaaaaa caagaattaa
    20761 ccagaatcga catagattcc ataaaaaatc ctgcactgcg gttttttgct tcccaactat
    20821 tgtggtataa gcataccaca atatataaat ggaaatacca tacttaccca actgagacgc
    20881 tattgaagaa aagttatcta actgaccagt caaagactgg tcaagagttt taacaataac
    20941 gtcatgagcg gcctggaaat acctagaagt tgccatagtg gctcctttta acttccatct
    21001 ttacccaata catttttgaa tgcatcttta tagtcaacat gcttaggatg taattgctta
    21061 tacctaaaca aaccttcctt agcgttttta caattttcgc tatcactccc agactttttg
    21121 cattcagcta cttttttcgt tgcatcgtct acgtgagact gccaccagtc tacagattta
    21181 acctcttcac acccggtaag aaagaatgta agccccaacg ccgcaactaa tccatatttt
    21241 ttcaaaatgc cccctcagtt catttcataa ctctgctgct caaatgctgc cttataatca
    21301 acaactggag tttctttatt aacagcccct aattgtttct cctggaaagc agcctgtaac
    21361 attttctcct tgtattctgc aagatcgcgc tgcttatcgc gatacatttc atactgaaat
    21421 ttgagcttgt taaatttcag gcttgccaga gatacagcgt ttgtcgcgtc ctgcgtggct
    21481 ttcgtgtcct gggcgttctt cacctggtcg ataaggtcgg ccatttcctg attatcttca
    21541 cgcagtttac tttccagctt attgccgtac tcgatccccg ccagttggga caggaacgag
    21601 gcttcacagc cccgcaccag gtcgcccgta tatcccagcc ccttacaggt gtttgtcacc
    21661 ttataatcgg ccagtagtga tctggcttgc tcagacaggg tggcttccgg gttcttaatg
    21721 taatcatcaa tgaaggagtt cccctggtcg taaatattct ccagctcctt actgacagat
    21781 tgcgcagact gcaccagtcc ctgaacatca cgaatgcccg ttttggtcag cagctcctgc
    21841 ttataggcat taatctgttt ctggtagtgg ttcacggtgt cctgccactg tttgaggcgt
    21901 tctgtccagc gctgcgcttc gacagcccat tcagggtttg catcaatggc aacgggaaca
    21961 ccagcctgag caccagaaat aaccgtaaca gaggcgataa gtgccgcaac gatatgcttt
    22021 ttcataggga ttccttaaat caggtttgca cgttccagat aggtatcaag ccactcttcc
    22081 ggcttcatac cctctttcca gatttcatcg aacagttcaa gctggggttt cgatgcgctg
    22141 agaaccggta ggtaatacgc cgcttttccc aaatccagtc ttgcgaaagc cgcaaaatca
    22201 tcactttccc ccttcctctg tgggtttttg acgaccagga actgacgaga taacgggtca
    22261 atggctttaa ttttgtcaaa ataaagttct ctcacttcca actcgtctac gtactcacta
    22321 cgtttcgctt tggggttcgc cagataaata tgtgttgcgc actgctcacg aacggcagcc
    22381 gcgataggtg atttaattag ttcatccggc gattgtgtcg ctacgacaag caccatatcc
    22441 agcttacgcg cggttttgag tttgttataa acaaaatctc tgaacgcttc gttgttaatc
    22501 cattgccaga actcatccat gtagataagg agtcggcggc catcagccag catggtgaca
    22561 cggtagatga gatagaatga agcggcgctg gaaagtactt tgttatccag aaactccgtt
    22621 ccgtctatac cgaaaacgtc caggttgtcc acatcaaacg tatcgtccgg gttatcaaac
    22681 acccagccaa attcccctcc ctgcacccag gcattcaggc gtgctttaag cccatgccgc
    22741 cgggtttcag tatcatcagg ctccagaatg agtggtcgca acttactgat cggataagag
    22801 cggtttgacc tctgcatcag gcgctcaacg gcatcagaaa tcaggcggtt ctggtaatca
    22861 tcaagcggct cactgttaag cgtacaaagt agcctcacca ggtctttcat aaaggaaata
    22921 tttcgtttgg ttggtggcaa agccgcaggg ttccagccag tcggctctcc ttctttcacc
    22981 cgaaagtaag cacctcccat tgaacgtata ccgggttccg ctgcccgatc tttgtcgaaa
    23041 aatactgtag ttaacttctt taatttcctc tcaggtggaa aagactccgg cgttccgaac
    23101 tgctgggcgg cgaaagcctt agtcatcatc actaccgttt tacccacgtt tgacgtacca
    23161 agaatttcgg tatgccccag cgtggggttt ttaccgaagt aattttgatg ctcggtagtc
    23221 atgtgatagt tcaggttata aatatcattt cctgagccgc ccagggtgat aaggttgtta
    23281 ccccaggtat ttcctttctc cttgcccgta aagaaattat ggaagctttc catttcggca
    23341 aagttcagac tgctgataga actcaggcgt ggccgtaacg tgtagttacc aggtaactga
    23401 gcaaaatacg ctgcgccaag actcaaggtt gaataggtta cgatcagccc taaatcctcc
    23461 agggtcgtcg ttacaatatt ggtgtccttc accagacgct ccggcgaatc tgcgtaaaca
    23521 atcagtgtct ggtgactttt cccgaaagaa atatagccgc tggagaccat atcgagacca
    23581 actttcaaat cagcgagcaa ggatttagcg gcatcatcgg tcatttcgag tttgtcgatc
    23641 tgatcatcca gcgccttgat tgcagactgc ttgtctatcg gagtaaaaga ggaagtctgt
    23701 acatactcaa ccgggagata catcagtgca tcaaaaattc cggcatccgt ttcctgaaag
    23761 tagtccttga tctcgatgca acgaaagtac cgggcatggt cagaggcagt gatttgtcca
    23821 gcatcgttac caaagaacag gtctttacca cccaggtatg tataaaacgg actattggtg
    23881 actctgactt tctgccagcg cccggatagc agtttctgga acagcgtaag ctgttctgaa
    23941 taaactatat tattttcttc atataaccca agacgacggg aatgaaaccg ggagagatag
    24001 gtgctcaatc tgccacatat ttcattcata tcattaatgg gttcttcgaa gatattttta
    24061 ttaccctttt tccttgaaag aaaatgcgtc actttatctt cagcgctgaa cggtttatag
    24121 catacggtta agtataattt gttttcaaaa tactcagccg ccgaaagcga ttcgtaataa
    24181 tcgttcatca ctctgttgac gaatgggatc ttgctgtcaa atacaggtcg cacttctttt
    24241 ctaacccgaa tacgatgcgt ataaaaggta acgggtttcc catcaaaact acgtatcagc
    24301 gtattgagct ggtcagtaag caaggctaga tccgcttcat ctacacattc aaaataagct
    24361 ccgtcaatct gccaggtcgc cagtagatca tggtttctgg taacaatgag gttatcagtg
    24421 atgtgtgatg aatacgggat taactcttca agagaagact ggtctttaag cttcataaaa
    24481 ttgtccactt tcgatatatc gactgcgtcg tacgaactcc ccgcataatg gacggcattg
    24541 aagcgcttgt ttgccagagg attacctttg atttgcattc gcagataaag caggtcaaaa
    24601 aagcgttcat ctatcttcgt aactcgcttg atggcatacc agccagggag gatgagggca
    24661 tacatagcct catgaatcca gacaccaagc aaaacgcaga taacgcacat tccaagaaag
    24721 gggtagagcg gaacgcccag ccccctaatc aaagcggggc gtgtcagccc tttaaaaacg
    24781 gtactcattt ttacgccccc ttttattaca tattacgcta accagtcagg cagatatggc
    24841 gcaacaataa agatgataat cccgataatt aaaggcgtaa tccacgctgc atgctggcgt
    24901 gagaagatag cgatataacc aattaccagc gcaatgatgg tgattgctgg cttacggata
    24961 tccgtcagaa aatcgataat cttctgcaag aagccagttg ttttagtgga tacatcatca
    25021 gcaaaggcgg gttctgctac acaaagcatc aatgcagcca taaatacaga aagagttaaa
    25081 taacgtttat tgagttttaa catcgtttgt ttccttttca tctaaaacag tgccacgcat
    25141 ggcatattgg ggatatacgg taacttcttt aggtttgata ctctgtgtta cattctcctt
    25201 tctgattgca tctttaactg aaggaaccac ccagtttttt ttgttatcag gcggattaaa
    25261 ccctatgcgc tgtatataac tggtgttatt aaaatctgat tcaggtttag ttcctgtttc
    25321 tggattgccg gaataatagc aactcaggcc acgtaaaata tcgccaccac gtttataaca
    25381 atccaccaaa atttgctctg aaaccttgag attttcgcag gggtcaaaca ttttctcagc
    25441 ggttgtattg aagtttgcaa aattcgtact cgttatttgc ataagaccga ctgagtaacg
    25501 atgatttcgt gattcaattt gatttacgat ctggagtgca gcctccttag ttttgggaaa
    25561 gtaggacact acgcctttgt caccgggctt acgttcaacc ttcggtatta tctcggcaat
    25621 agcatatggg ttaaaaccag attcaaccct tgcaacttca tgcgcagtat cgggatgaac
    25681 gctggccgcg cattgcaaag caagcgccgc aaaagcagta gtagataaca tgattttctc
    25741 ccggccagaa cagcgcggaa agtttagact gaatggttag tcatcgaagt cgtcatcgtc
    25801 ttcatcgtct tcgtttatca gctcctcaat agcctggctg gaatattttt ttatgaggta
    25861 ctggacaaca ccagagcttg aaatacgtcg ccccctgcga tagccaatct ccagagcaat
    25921 tttatccagt tcctcacgag aagctgaggg caggtagagt gtattacgtt ggggcttgcg
    25981 tctcgttgcg gttttttttc gggtagcagt catttttctt ccgtttcatt ttgtgggtta
    26041 agcttctctc tgtgatgtag tggatgaaag ccatacttct ttgctgcgga ttatccatga
    26101 gtagaatttc agcaaaatcg tgtggaattg atttccttaa aagatttgcc tgagctgttg
    26161 tatgtgattg tctgtacaaa agctccgcca tcacatcttc tggtacaggt aaaggctcac
    26221 cgccaaatga gataaagcgt tgtacgtaag ggatagcagt tattgtagtg gaatgaacct
    26281 cttcaaaatc tgcctggata aaaagataac cgggaaaaag cggcaaacgt ctttctcgat
    26341 atgaaattat cttatccggg cgacgatacc tttcagtctc cataggacag aagtaagaga
    26401 agttcaggcg ttcgagatga gcaaaaactg ctgcaaatct cgtagtggtg aattgcaaaa
    26461 cataccattc catagttttt cctttcaggc gctctctcga ctcggatgac cccgaatcag
    26521 agaaaaaaaa caaaaggaca aatttacatc tcataaaaac tttatcagct acatgtgctg
    26581 cgttcaacca cacaagcaga tctttagatc gcatttacga cacatttcat tttgtttg
//
LOCUS       DAJSKJ010000035        26541 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000035 DAJSKJ010000000
VERSION     DAJSKJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.035"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(67..1194)
                     /gene="rlmC"
                     /locus_tag="ON345_001287"
     CDS             complement(67..1194)
                     /gene="rlmC"
                     /locus_tag="ON345_001287"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR3071374.1"
                     /translation="MQCALYDAGRCHSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSCPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(1235..1723)
                     /locus_tag="ON345_001288"
     CDS             complement(1235..1723)
                     /locus_tag="ON345_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR3071375.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(1783..2628)
                     /gene="potI"
                     /locus_tag="ON345_001289"
     CDS             complement(1783..2628)
                     /gene="potI"
                     /locus_tag="ON345_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR3071376.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(2625..3578)
                     /gene="potH"
                     /locus_tag="ON345_001290"
     CDS             complement(2625..3578)
                     /gene="potH"
                     /locus_tag="ON345_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR3071377.1"
                     /translation="MSTLEPAAQPKPPGGLRLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDSSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(3588..4721)
                     /gene="potG"
                     /locus_tag="ON345_001291"
     CDS             complement(3588..4721)
                     /gene="potG"
                     /locus_tag="ON345_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR3071378.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDPDASVVDNVPVHVALRPEKIMLCEEPPADGYNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(4816..5928)
                     /gene="potF"
                     /locus_tag="ON345_001292"
     CDS             complement(4816..5928)
                     /gene="potF"
                     /locus_tag="ON345_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR3071379.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(6279..6755)
                     /locus_tag="ON345_001293"
     CDS             complement(6279..6755)
                     /locus_tag="ON345_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR3071380.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(6843..7745)
                     /gene="rimK"
                     /locus_tag="ON345_001294"
     CDS             complement(6843..7745)
                     /gene="rimK"
                     /locus_tag="ON345_001294"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR3071381.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTTITFYGTASLRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(7806..8528)
                     /gene="nfsA"
                     /locus_tag="ON345_001295"
     CDS             complement(7806..8528)
                     /gene="nfsA"
                     /locus_tag="ON345_001295"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR3071382.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(8512..8799)
                     /locus_tag="ON345_001296"
     CDS             complement(8512..8799)
                     /locus_tag="ON345_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR3071383.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            8959..9216
                     /gene="grxA"
                     /locus_tag="ON345_001297"
     CDS             8959..9216
                     /gene="grxA"
                     /locus_tag="ON345_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR3071384.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(9246..9623)
                     /locus_tag="ON345_001298"
     CDS             complement(9246..9623)
                     /locus_tag="ON345_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR3071385.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            9893..11578
                     /locus_tag="ON345_001299"
     CDS             9893..11578
                     /locus_tag="ON345_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR3071386.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(11753..12289)
                     /gene="rcdA"
                     /locus_tag="ON345_001300"
     CDS             complement(11753..12289)
                     /gene="rcdA"
                     /locus_tag="ON345_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR3071387.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            12373..13581
                     /locus_tag="ON345_001301"
     CDS             12373..13581
                     /locus_tag="ON345_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3071388.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13581..14396
                     /gene="ybjI"
                     /locus_tag="ON345_001302"
     CDS             13581..14396
                     /gene="ybjI"
                     /locus_tag="ON345_001302"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR3071389.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            14482..14766
                     /locus_tag="ON345_001303"
     CDS             14482..14766
                     /locus_tag="ON345_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071390.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(14807..16039)
                     /gene="mdfA"
                     /locus_tag="ON345_001304"
     CDS             complement(14807..16039)
                     /gene="mdfA"
                     /locus_tag="ON345_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR3071391.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            16324..16920
                     /gene="ybjG"
                     /locus_tag="ON345_001305"
     CDS             16324..16920
                     /gene="ybjG"
                     /locus_tag="ON345_001305"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3071392.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            16978..17736
                     /gene="deoR"
                     /locus_tag="ON345_001306"
     CDS             16978..17736
                     /gene="deoR"
                     /locus_tag="ON345_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR3071393.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(17783..18985)
                     /gene="dacC"
                     /locus_tag="ON345_001307"
     CDS             complement(17783..18985)
                     /gene="dacC"
                     /locus_tag="ON345_001307"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR3071394.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19232..19858
                     /gene="gstB"
                     /locus_tag="ON345_001308"
     CDS             19232..19858
                     /gene="gstB"
                     /locus_tag="ON345_001308"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR3071395.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(19855..20970)
                     /gene="yliI"
                     /locus_tag="ON345_001309"
     CDS             complement(19855..20970)
                     /gene="yliI"
                     /locus_tag="ON345_001309"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR3071396.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21081..21464)
                     /gene="bssR"
                     /locus_tag="ON345_001310"
     CDS             complement(21081..21464)
                     /gene="bssR"
                     /locus_tag="ON345_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR3071397.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEVLATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            21677..23002
                     /gene="rimO"
                     /locus_tag="ON345_001311"
     CDS             21677..23002
                     /gene="rimO"
                     /locus_tag="ON345_001311"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR3071398.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23049..24377)
                     /locus_tag="ON345_001312"
     CDS             complement(23049..24377)
                     /locus_tag="ON345_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR3071399.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQIVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGNVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(24385..>26541)
                     /locus_tag="ON345_001313"
     CDS             complement(24385..>26541)
                     /locus_tag="ON345_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR3071400.1"
                     /translation="ALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYY
                     LFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNC
                     FDRPGKKCISFTLRINDKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHE
                     IIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNA
                     SDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIRE
                     GDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGI
                     IKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTF
                     TRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFI
                     FCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAER
                     GIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLN
                     EPHFHERFTTIMKENGLKADSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFG
                     TGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRNPVIAEGVESED
                     VARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         6750 a         6871 c         6353 g         6567 t
ORIGIN      
        1 acacgccgca tccggcatga acaaagcgca cctgatcagc aatctgaagc cgcacctgcg
       61 gcttttttat tgcttcacca gcagcgtcag cacttcatag tgcgcggtgt gcgggaacat
      121 atcaaaaagc tgtacccgtt cgatgcgaaa cccaggcagt tcgcggatat ctttcgccat
      181 ggtttgggcg ttacagctgg agtagataat aaaacgcggt gccatcgttg agagataatc
      241 acacagcggt ttaccaatgc cgcggcgcgg cgggttaacc agcaccagct ccggcacctc
      301 cccctgagca gtggcaaact gagtggagtc cagcgcctga aattgtaaac gagttaagcc
      361 cagttcagcg gctgactgct ttgcacaggc aatggcctct ggcgcaattt cgatcccggt
      421 taactgcatg tcaggcgtcg cgcagtgtaa accaaagccc cccacaccgc agaacagatc
      481 ccacatatgt ttaaccggca gctgtcgtac ccagtcgcgc gcggtggcgt acaactggct
      541 ggcgaccgcc ggattagtct ggaagaaact ttgcggacgg atccacagcg gtacgtcatt
      601 aaaacgctcc gccagtgcct gttgttcggt caggtagatc tccgtctccc cttccataat
      661 cgccatatgt accggctgaa tattgacggt aataactttc agctgcggta gttgttcgtg
      721 taaccacggc agcgccttac gcagttgcgc cagcttggta tcagaacgca gtacaaagcg
      781 cagcatcatg ccgccatcac tctggctttc agtcagcaga atgtatttca gttcgccacg
      841 cttacgcgcc acgttgtagg gcgttaaccc cgcacgggcg ataaacggtt ttagcgccgc
      901 aaaaacgggc gcaaaagagg caggataaag cgggcagtca caaaggtctt ccggcgtgcc
      961 atcgcgatgc agcataccga gcagtggttt ttcaacgcta ccactcacca ccattttggc
     1021 tttattacga aacccttgtt ccgggcatga caccggcgcg caccattcct caaccggaaa
     1081 atcggcgagc agatttttaa gatcggcggt tttagcggag agttgctctg gaatcggctg
     1141 cgttatccac tgacaggaat gacagcgacc cgcgtcgtaa agtgcgcact gcatacattg
     1201 accttcacat catcaggggc gacgattata cacattattg caactggaag aaccgccgac
     1261 tggtcgaggg aacgaacagc agcatcagga tgagcatatc cggcagcttc tgcagcatca
     1321 ggctatggaa gatttcacgt tttgattcac cgggaatgct gaacagctcc ggataaccgt
     1381 accccagcga agccgcccac aagtaacttg cggcggtgat ttgtgtcagc agatatagcc
     1441 agcgcgccca gcgacgccct ttcaccagtg aaaacgcaca ccagatctcg ataaaaacca
     1501 gcaccagact gcttaaaaat accagcgtta agctccaggt ttgtacgctg cgatgaatga
     1561 actcgccaat accgcgcacg cccagcgtat tgaaaatcat cagcacgtcg aggccacgga
     1621 tcataataat ggcgagcgcc gctacctgca ccagcgcagg cacattcagg cgagcatgag
     1681 atgaagatgt tttcttaaaa aatcccaacg tttcgtcttc catgaaaaca atgccgcggc
     1741 atgcgcggca ttatgtagcc aggttggcaa attttagtgt cttcagccat gtcttgcacg
     1801 ctggatatcg cgtatccgct gtttttctgc gcgagccatc agataccagg cgataaatcc
     1861 gacaattccg accgcaccga gaattaacgt tgcaagggcg ttgatttccg gattcactcc
     1921 catccgcacg ctggaaaaga ccagcatcgg taacgtggtg gctcccggcc cggaaacaaa
     1981 gctggcgatc accagatcat caagcgacaa agtaaaagcc agtaaccagc cagaaatgat
     2041 cgcgggcatg atcatcggta gcgtaatgac gaaaaacact ttcagcggcg tcgcaccgag
     2101 atccatcgct gcttcttcta tcgagcgatc cagttcccgc aatcttgaag aaataacgac
     2161 cgccacataa gccgtacaaa aagtgacatg cgccagccag atggtgagca taccgcggtc
     2221 cgcaggccag ccaatggcat gagcaagcgc gacgaataat aacaacagcg acaagcccgt
     2281 gatgacatct ggcatcacca gcggcgcggt gatcataaag gcaaaaccat ttgatccgcg
     2341 aaacctgcca aaacgcacca gcaccaccgc cgcaatagtc ccgaggatcg ccgccgccgt
     2401 tgccgcacag gcagcaattg tcaggcttaa accaaccgca ctcatcatcg cgtcatcgcg
     2461 caataactca ccataccagc gcgttgacca gccggcccac accgtcacca gcttcgagct
     2521 gttaaacgaa tagataacca gcatcagcat tggcgcgtag agaaaggtaa agcccagcag
     2581 caaaatcaca atccgccagg gcgaacgaac taccggtaaa ttattcatcc gtgttctccc
     2641 acgctttttt gctggtgttt gtggaaccac attatcggca caattagcag cagcaacatg
     2701 atgatcgcta ccgccgaggc caccggccag tcgcggttat tgaaaaactc ttgccatagc
     2761 acgcgcccga tcatgatgct gtccgggcca ccgagcagtt ccgggatcac aaactcgccc
     2821 acagccggga taaacaccag catcgatccg gcaataatcc cacctttagt cagcggcacg
     2881 atcacagtaa agaacgtttt cagcggtcgt gcaccgagat ccagcgctgc ttccaccagc
     2941 gaagaatcaa tacgaatcaa cgcggtataa atcggcagca ccataaacgg cacgtaggcg
     3001 taaacaatgc caatataaac tgccagattg gtatgaagaa tggtcagcgg ttgatcgata
     3061 acccccagcc acagcagaaa attattcagc acaccgttgt tttttaatat tcccatccag
     3121 gcataaacgc ggatcagaaa cgaggtccac gacggcagga tcaccagtag taataaaata
     3181 ttacgggtcg aaggcttact gtgcgccacc gcccacgcca gcggatagcc gatcagtaaa
     3241 cagcaaaatg tcgaaatcgc agccacctgt agcgactgga gataagcatc gaaatagagc
     3301 ggatcgtcag tcagttgcag aaaattaccg agattaagag tgatggacag ttgcccgtca
     3361 gcccactcca tcagttcggt ataaggcgga atggcgcgcg ccatctccgc cagacttatt
     3421 ttaaagacga tcagaaatgg cagcagaaac agcaggatca accagatata cggcaacgca
     3481 atcaccagtt tgcgcccgtg cttcatttgc aactgcgaca gccacagcct taatccgccc
     3541 ggcggtttcg gctgggctgc aggttcaagt gtactcatct ttgctcctta aaccgtcagc
     3601 accacacaac tgtctacctc ccagcacaaa cgtacttcat cgccccaggt tggtaaccct
     3661 ttgcgatatc gatgagcgtt ttgtagctga gcgctgatca tctgtccgct cttgagacga
     3721 acgtgataca ccgaaagatc gccgagatag gcaatgtgca tcacctcacc taccgcaaag
     3781 ttataaccgt cagcgggagg ttcctcgcaa agcatgattt tttccgggcg tagcgccaca
     3841 tgcaccggca cgttatccac tacagaagca tccggatcaa ctttcaatgg atgcaccagc
     3901 ccaggcgaat aaagcacaag cccatcttca tgacgctctt tgagtacacc ttcaaagaca
     3961 ttaaccgaac cgataaattc agcactgtag cgggtggttg gatgttcgta gatctcttct
     4021 ggctcgccaa tctggacaaa tttcccgcga ttcataatgg cgatacgtcc cgccatcgtc
     4081 atcgcctctt cctgatcgtg agtgaccatc acacaggtca cgccgacacg ctccagaata
     4141 tccaccactt caagctgcat ccggtcacgc aattttttat ccagcgcgcc catcggttcg
     4201 tcgagcagca gaagttttgg gcgcttggca aggcttcggg ccagcgccac acgctgtctc
     4261 tgaccgccgg aaagctgatg cggtttgcgt ttggcgaact cctgcatatg caccagccca
     4321 agcatctcgt tgacccggct ggcaatttcc gctttcggta gtttgtcctg tttcaggcca
     4381 aaggcgatgt tctgctccac ggtcatatgc ggaaacagcg cgtaagactg aaacatcata
     4441 ttgatggggc gcaggtaagg cggcacctgt gacaaatcga cgccatcgag cattatctgc
     4501 ccggcagaag gttgttcaaa acctgccagc atacgcagca gcgtggactt gccacagccg
     4561 gatgcgccca gcaacgcgaa gatttcacct ttgtagatgg tcaggctgac atcatcgacc
     4621 gcatgctgac catcgtagga tttggtgagg ttgcggattt ctaatagcgg cgtcagcgcc
     4681 ttacgggttt tcgcctgcgg gcgagggata gcgtcattca cggctgctct ccggcattga
     4741 atgtgtgcaa gcgcaccatg atggtgcgaa ttgccggcgg gtgtggtgcg cccgcccggc
     4801 atctaaggct gcggattatt ttccgctctt cactttggtc cacgcgcggg tgcgcacacg
     4861 gtcgattttc gggtcctgca ctttcagagt gaacagcttc gcacgaacat ccgcaggcgg
     4921 ataaataccc gggttctcac ggacttccgc actcaccagc ggcgtggctg ctttattggc
     4981 gttggcatag aacacatggt cggaaatatg cgctactaca tccgggcgca gcaggtaatt
     5041 caggaactga taggcttcgt ctttgttttt ggcatccgca ggcatggcga atacatcaaa
     5101 gaacgccatc gccccttctt ttggaatcga gaacgagaca ttcactccat tcttcgcttc
     5161 cttcgcgcgg tttgacgcct gccagacatc acctgcccag ccgatagcga cgcaaatatc
     5221 gccgtttgcc aggtcgttaa tgtattgaga tgaatggaaa taacgaatgt tcgggcgcag
     5281 ctttaacagc agatcggttg ccggtccggt gtaatcatcc gctttagtgc tgttgggatc
     5341 tttgccgaga taattcaaca cggtagcaaa aacttcttct ggcgcatcca ggaaagagac
     5401 accgcagctt ttcagttttt ccagattttc aggtttgagg atcaagtccc agctatcgac
     5461 gggcgcgttt tcgcccagca cagctttaac tttatcaacg ttatagccaa tcccggtcgt
     5521 cgcccacata tagggcatag caaatttatt gtcgggatcg tgtttggcga ccagcttcag
     5581 cagctccggg tcgagattct tccactccgg caatttgctt ttgtccagcg gctggaaaac
     5641 tcccgcagtc aactggcgtt caagaaagct ggcagatgga accaccaagt caaaaccggt
     5701 gctacccgcc attaatttgc cttccagtac ttcgttagag tcgaaaacat cgtagacgac
     5761 tttaataccg gtttcttttt caaaattggc caccgtgtcc ggggcgatat aatcagacca
     5821 gttataaatg tggagtgttt tttgttcagc cgcgagcgtg ccgacagaga cggccatcag
     5881 agcacccgca accagacccg aaagccattt tttatttaag gcggtcatat ctctttcctt
     5941 ctgaaagttc gttaacaaac gaacaaaagt tgtcacacta aaagatatgc aataaacgtg
     6001 catatttttt atcggcgcgg tttaagaaaa gagaactctc tcccggccag cactgctcgc
     6061 tatataaaag catcgcaaga ataactttag ccagagtttg agactatagc tcggattaaa
     6121 aaacggtatt taacaaattt ttatgcgtat ttgctctatg tgataaagcc aaatcggcac
     6181 aagagaagtg aaataatctt taagaaaagg ttaaaaacag cagataattt tgcagcgcgc
     6241 agtcactgca ccagcgactg cgtgctcgaa aggagttatc agtggaggaa atagttttcg
     6301 gtaacgttgt tttgcccttc atcgtcagtc ggtaacagca gttgatgggc attagcttca
     6361 agaatgacca tcgaaatctg ctcttcgctc tgacgcacga accatgcaaa ctgctcatag
     6421 gttacgccct gcataacgga taaagactgg caaaccacca gctttggcag attatcatcc
     6481 tgcatatcaa gaaatgcttt cacggtcagc gaactggcat tgatggcaga taaatccgcc
     6541 gccagcggca atacggctga aggtcggact tccgccatag cagaaaacag gatcgtgtta
     6601 tcgatcagat cgattttggc atcaaagaca ccgtcgaaat tctgcatatg gggcagatgc
     6661 agagcctgac agttatcaca ttcaaaaaaa ctcatcccca ggtcatcgag ccattgacgc
     6721 agcgtatcca gaccaggaac gaccagcgat gtcataattt tgtacaacct ctttcgataa
     6781 aaagaccggc acagcttacg caaaaagcgc aggcaaaacc atgatcagta atgtgattgc
     6841 gactaaccac ccgttttcag gcaatattcg gtcgtagcgt ggcgttcgat ccagcggatc
     6901 attttacccg cgatgtcgat accggtggtt ttttctattc cttccagccc cggcgacgca
     6961 ttcacttcca tcaccaacgg cccgcgatta gcacgcagaa tatccacacc agcaacgtcc
     7021 agcgccatcg ttcgcgcggc ttttatcgcg atttcacgct cctgtggtgt gatactggcg
     7081 acgcttgccg cgccgccacg atgcaaattg gaacgaaaat cgccctcttt cgcccgccgt
     7141 tcaatcgcag cgaccacttc atcgccaaca accagacagc ggatatcgca cccttgcgcc
     7201 tctttgatat attcctgcac cagaatatgc gcgttcagac cgcggaaagc gtcaatcacg
     7261 ctttccgccg cctgacgcgt ctccgccagc acgacgccaa ttccctgcgt gccttcaacc
     7321 aacttgacca ccagcggcgc accaccgacc atgtcgatta aatcgctggt atcatccggc
     7381 gaatgcgcaa tgcccgtgac aggcaggtcg atgccctgac gcgccagcag ttgcatggaa
     7441 cgcaatttgt cacgcgcccg ggcaatggcg accgactcat tgagcggata gctccccagc
     7501 atctcgaact ggcgcagtga cgccgtccca taaaaggtaa tggtggtgcc aatacgcggg
     7561 atcactgcgt caaaatgggg taacttgcgg cctttgtagt gaatagaaga cgccgcagga
     7621 tttatgttca tgtagcaaga aagcggatca agaatttcaa ccaggtgacc gcgctgtata
     7681 gcggcttcac gcagccgctt acacgaatag agcgttccat cccgggacaa tatggcaatt
     7741 ttcaccctgc acctctctgt cagacctggt caaaagcccg cgtatcatac atcgacgtgg
     7801 cggttttagc gcgtcgccca accctgtttg tgcaaataat ccagaataaa tgggcggctt
     7861 tctttaatga ttgttcggcg gatatgatcg ctccaggtat cccggcgatt attgctgcca
     7921 cgggtgagat aatattccgc cagttgctcg tcatactgag ccagtgcgcc tttatccagc
     7981 ggttgatagc tgttttcatg caccaaaatg gaggccggta aacgcggctt aagatccgga
     8041 ttatccgcag gccagccaag gcacagccca aacagcggca gaacatgctg cggtaattta
     8101 agcagtttcg tcaccgcttc aatattattg cgcaggccgc cgatatatac cccgcccaat
     8161 cccagcgatt ccgctgcgat taatgcattc tgcgccatca ttgccgtatc aacgacaccg
     8221 agcaacagtt gttccgccag gccgagctga gcatccggac agatctgtaa atggcggtta
     8281 aagtcggcac agaacaccca gaactccgcc gcttgcgcta cgtgtttttg cccgccggtc
     8341 agcgtcacca gttcttcacg taacgctttg tcggtaatgc gaataatgct actgcactgc
     8401 aaaaaactgg aactggacgt cgcacgggcg ctgttaataa tcgcctcacg ctgcgcttcg
     8461 gaaatgggtt catcagtgaa atggcgaatg gagcgatggc cacaaataag ttcaatggtt
     8521 ggcgtcatta tctttttctc tttctgaacg tgaatattgc ggtggacggt tcatcagctg
     8581 tggggcaaga cgttttgcca cctgaagaat aaccaccacc gcagcgggaa gcatgagcaa
     8641 aacaccgaga aaaatcatca gaatctgcac ttctggccga gaaaatggct caggcagcga
     8701 cagggagtcg cttaccgaca gcagcgccac cgccagtagc atcattccga taaattccag
     8761 tatcaacacg cctttaggca atttaccgat cgcgcgcata cgcttccctc tgcaaagtga
     8821 gccttcagtc taaaactttt cactgtattg tgtttaacag ttatagcttt tagcaattaa
     8881 tgcaacaggt taaacctact ttcagcgaat acattttagc gtgatcatta caggcataaa
     8941 tctatgagga gagaaataat gcaaaccgtt atttttggtc gttcgggttg cccttactgt
     9001 gtgcgtgcaa aagatctggc tgagaaattg agcaatgaac gcgatgattt tcagtatcag
     9061 tatgtagata ttcgtgcgga agggatcact aaagaagatc tacaacaaaa ggcaggtaaa
     9121 cccgtagaaa ccgtgccgca gatttttgtc gatcagcaac atatcggcgg ctataccgat
     9181 tttgctgcat gggtgaaaga aaatctggac gcctgatcgt ctgacaagcc ctcgcgttga
     9241 gggctttact gattttttct gtgctgtggt ttaaacaaac tactgataaa taagaaacac
     9301 agtgccccca gcgcacacca gaacaccgcg cttagtaacc atgccagctc ttgccagaat
     9361 gagcgcgtcg gtgaaaaaaa cagccgcata atgagcatcg aacagggtgc cgccagcatt
     9421 gcgccaaaca gaggtttcag gacttctcta cgctgtgaaa agaagctggc gactgctcca
     9481 ggaagaatga aaaatagcaa gccgatttca ggatgcccgg cagcccgaaa agcgcctttc
     9541 atgtgcgtcg ccagaaaaag gcacaccaca atgaagagga caaaacagca gattgccccc
     9601 gcccaacgtt gtttatgttt cactcgttcc tcctgacact gcgtctatcg aacacatttt
     9661 tcgccagtgt ggcgttcagt aagataaagc cgcttcgcat tccatgctaa tataggccaa
     9721 cgcaattcat atagccgttg atacctaatg tgattacact agtaaaataa attgttactt
     9781 tactatcgtt taggtgcgct gaatgaatct gcgccctgaa ttctggtaaa aaacattatc
     9841 gtaaattacc atttctttca acagcttact agtaaacaag aagttagcct ccgtgaatat
     9901 aaacgtcgcc gaattgttaa atgggaatta cattctgtta ttatttgtgg tcctcgcgct
     9961 tgggctatgt ctcggaaagt tacgacttgg ttcgatccaa ctgggtaatt ccattggcgt
    10021 tttagtcgta tcgctgttat taggccaaca acatttcagc attaacaccg atgcgcttaa
    10081 tcttggcttt atgctgttta ttttctgcgt cggggtcgaa gccggaccga actttttttc
    10141 catttttttt cgcgatggga aaaattacct aatgttagca ctggtgatgg ttggcagtgc
    10201 gctggtgatc gccttagggt taggtaagct gtttggctgg gatattggcc tgacggccgg
    10261 tatgttagca ggctctatga cgtcgacacc ggttctggtc ggtgctggcg atacactgcg
    10321 tcattccggc atggaaagca ggcagctctc actggcactg gataatctga gcctcgggta
    10381 tgccttaacc tatttaatcg gtctggtgag tttgattgtt ggtgcgcgtt acttgccgaa
    10441 attgcagcat caggacttac agaccagcgc ccagcaaatc gcccgcgaac gtggcctgga
    10501 cactgatgcc aaccgtaagg tttatttacc ggtgatccgc gcctatcgcg tcggcccgga
    10561 gctggtggcc tggaccgacg gcaaaaatct gcgtgaactg ggtatttatc gacaaaccgg
    10621 ctgctacatt gaacgtattc gacgtaacgg gattctggca aatccagacg gtgatgccgt
    10681 gctacaaatg ggcgatgaaa tagcgttggt aggctatccc gacgcccatg cccgactcga
    10741 tcccagcttc cgtaacggta aagaagtttt cgatcgtgac cttctcgaca tgcgtatcgt
    10801 cactgaagaa gtggtcgtta aaaaccataa cgctgtaggt aaacgtctcg cacaactgaa
    10861 gttgaccgat cacggttgct tccttaaccg cgtcattcgt agccagattg agatgccgat
    10921 agatgacaac gtcgtgctta acaaaggtga cgttttacaa gtcagcggtg atgcccgtcg
    10981 cgtaaaaacc atcgccgatc gcatcggctt tatctcgatt cacagccagg tcactgacct
    11041 gctggcattt tgcgccttct ttgttattgg gctgatgatc gggatgatca ccttccagtt
    11101 cagcacattc agtttcggca tggggaacgc tgccgggttg ttattcgccg gaattatgct
    11161 gggctttatg cgtgctaacc acccgacctt cggttacatt ccgcaaggtg cattaagcat
    11221 ggtgaaagag ttcggcttga tggtgtttat ggcaggcgtt ggtctgagcg ccggtagcgg
    11281 tattaataac ggcctgggcg cgattggcgg tcagatgttg attgccggat taattgtcag
    11341 tcttgtgccc gtggttatct gtttcttgtt cggtgcttat gtattgcgaa tgaaccgcgc
    11401 actgttgttc ggcgcaatga tgggcgcacg cacctgcgcg ccggcaatgg agatcatcag
    11461 tgatacagct cgcagtaaca tccctgcgct gggctatgcg ggcacctacg caatcgccaa
    11521 cgtcctgctg acgctggcag ggacaatcat cgtcatggta tggccaggat taggataaaa
    11581 ctgaagttgc cctgaaaatg aaattttttt gcacaaccgc agaacttttc cgcagggcat
    11641 cagtcttaat tagtgccact gcttttcttt gatgtcccca ttttgtggag cccatcaacc
    11701 ccgccatttc ggttcaaggt tgatgggttt tttgttatct aaaatttatc tactaccctg
    11761 caaccctctc aaccatcctc aaaatctcct cgcgcgatag cggcttacgg tcggtgacaa
    11821 aatgcagcgt catcccctca ataaacgcat caagcgcgcg ggcggttccg ggttcaaacc
    11881 attgttcgag cgtttgctga ctgcgctgca tccagttttg cattaccgtt tttaatagcg
    11941 gttttcggct agccagcgcg tagagctggt acatcagctc catattatcc ggcgttgcaa
    12001 cctgtgagct gtagatcata tcggtgatag cctggcatgc gccttgagca tcactaacat
    12061 cgctaaaaaa tgcctgatat tgccgggaca taatctcagt aaaactgctg aacgcctcca
    12121 gcaacaactc atcaattcct gaaaaatagt aggtcatcga tcccaacggt accccggcaa
    12181 gggtagcaat tttgcggtgc gtaacagcat gtattccgta aagtttcacc gcctccagcg
    12241 tggcctggat aattttttct cgccgttgcg gatcgttagc gcgacgcatg tcatttcctc
    12301 tgtctgcaat tgtgtacaaa tgtacacaac cttgctaatg ttgtcttccc tcttcttatt
    12361 ttgacgtgat ctatgaccgt aaattcttca cgtaatgcat tgaaacgccg aacctgggcg
    12421 ctgtttatgt tcttcttttt gccaggcctg ttaatggcgt cctgggcaac ccgtacgcct
    12481 gctatccgcg atattctctc tgtctcgatc gctgaaatgg gcggtgttct ctttggtctg
    12541 tcgatcggtt cgatgagcgg tattctctgc tcggcgtggt tagtgaaacg ctttgggaca
    12601 cgtaatgtca tcctggtcac gatgtcctgc gcattgatcg ggatgatgat attaagtctg
    12661 gcactctggc tgacatcgcc cctgctcttt gccgttggtc tcggcgtctt tggggcaagt
    12721 tttggttctg cggaagtggc gataaacgtt gaaggtgctg ccgttgagcg agaaatgaat
    12781 aaaacggttt tgccgatgat gcacggtttt tatagcctgg gcacgctggc aggcgcaggt
    12841 gtcgggatgg cactgacggc ctttggcgtt ccggcaacgg tgcacatttt attggcggcg
    12901 ctggtaggta tcgcgcctat ttatatcgcc attcaggcaa tccctgacgg tacgggcaaa
    12961 aatgctgccg atggcaccca gcatggcgaa aaaggcattc ctttttatcg cgatatccag
    13021 ttgctgttga ttggtgttgt agtgctggcg atggcctttg ccgaaggttc tgccaacgac
    13081 tggttaccct tattaatggt tgacggtcac ggttttagcc ctacttccgg ctcgctgatt
    13141 tatgccggtt ttaccctggg gatgaccgtt ggacgcttca ccggcggttg gttcatcgac
    13201 cgttacagtc gcgttgccgt ggttcgggcc agtgcactaa tgggggcgtt gggtattggg
    13261 ctgattattt ttgtcgatag cgcctgggtc gcaggagtgt ctgttgtact ttggggactg
    13321 ggtgcctcgc tgggcttccc gctgaccatt tctgccgcca gcgataccgg ccccgatgca
    13381 ccaacccgcg tcagtgtggt agcaacgacc ggttatctgg ctttcctcgt cgggccgccg
    13441 ctgctgggct atctcggcga acattatgga ttacgtagtg caatgctggt tgtactggcg
    13501 ctggttattc tcgcggctat tgtcgcgaaa gccgtcgcca aacccgatac caaaacgcag
    13561 acggcgatgg agaatagttg atgagcatta aattaattgc ggtagacatg gatggtactt
    13621 tcttaagcga tcaaaaaacc tataaccgtg agcggtttat ggctcagtat cagcaaatga
    13681 aagcacaagg cattcgcttt gtggtcgcca gcgggaatca atattatcag ttgatctctt
    13741 tcttccctga aattgcgaat gaaattgcct ttgtggctga aaacggcggc tgggtagtga
    13801 gtgaaggcaa agatgttttt aatggcgagc tgtcgaagga tgcctttact actgtcgtgg
    13861 aacatttgct gacgcgcccg gaagtggaaa ttattgcctg cggaaaaaat agcgcctata
    13921 cgctcaaaaa atatgacgat gccatgaaaa cggtggcgga aatgtattat caccgtctgg
    13981 aatacgtcga taactttgac aacttagaag atattttctt taagtttggc ctgaatcttt
    14041 ccgatgaact gattccacaa gtacaaaaag cattacatga ggccatcggc gatattatgg
    14101 tgccagtcca caccggcaac ggcagcatcg atctgattat ccccggcgta cataaagcca
    14161 atggccttcg ccaactgcag aaattatggg gaatagacga cagcgaagtg gtggtctttg
    14221 gcgatggcgg taacgatatt gagatgctgc gtcaggcagg ctttagtttt gcaatggaaa
    14281 atgccggcag cgcggtcgtc gcagcggcaa aataccgggc aggctccaat aaccgtgaag
    14341 gcgtactgga tgtgatcgat aaagtgctta aacacgaagc gccatttgac caataaaaac
    14401 ggcagcacgc ccggcaacac aatacctgaa tgaaggctgc ataattcgcg gaaattaacc
    14461 cgctgcatta tttcaggcga aatgattatg aaaaattgtc tactgttggg cgcgctttta
    14521 atgggcttta ctggtgtggc gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac
    14581 aaagtggttg tagttcctga ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg
    14641 ccgcaaaccg tctatgtggc tcctgcgcca gctccggctt accgcccgca tccttatgct
    14701 cgccatcttg ccagcgtagg tgaagggatg gttatcgaac accagattga cgaccatcac
    14761 cattaattcg agaatgcctg atcgcacaaa gcagtcaggc atttttttac ccttcgtgag
    14821 aatttcccat ctgtttatct tttaaaaaga taaccatcag cgacagccac aaaattccgt
    14881 tgacaaggtt gaagagatta aactgtccgt tgcccccgtt cagccaggca tgtttgctga
    14941 tttcaatacc aacggtaaag atcagcattt gcagcattcc catcgccgca gaaaccgtac
    15001 ctttactcat atcgctggca aacagggtta atcgcaccag tcccgcattc gccagaccaa
    15061 taccgaaagc ataaatactt aacccggcag tcatccataa atacgcgtgc gatgagataa
    15121 ccgttgccgc agcagcgacc aacagaccaa tcataatcgg ccagccgccc ataattatca
    15181 gcgaacgtac ggtgcggcgc gaggtcagac gcgctaacag caagttaccc gcaattaacg
    15241 ccccgaaaat aggcacttgc agcaagccat attcatagct gctcaactgc tcgccggtaa
    15301 tgatgataat cggcgactgg gcgatccacg ccagcaatgg caggctaacg aatcccagcg
    15361 ccagcgcccc cgccacaaag cggccgttct tcagcaccag cttatagtca cgaccgagtt
    15421 ctttcagtga cagtttctcg cctatacgcg tggcggtttc aggcatagct cgttgcagac
    15481 cgaaaaagga gatcgctgcc aatgcggcaa acaaaacaaa catcccctcc cagggcagca
    15541 catggatcca cgccgcgccc accagcggac caagtagcgg agcaatcagc gccacgttcg
    15601 ccatcagcgc ggtgatcttg atacaaaccg cctcttcgaa ggattcctga attgcggcgt
    15661 atcccacagc gccaatgaaa cagaggctta tgccctgcaa gaagcgcaac agggtgaatt
    15721 gttcaatgtt ttgcgccagc aatattgcca gacaggtgat gataaaccac accacccccg
    15781 ccagcatcac cggacggcga ccaatacgat ccgacagcgg ccccaggagc cattgtaaaa
    15841 acatcccgcc cgccagatat gcggtcattg aagtaggaac ccaatcaatg cccgcctgat
    15901 attgttccac cacggccaac ataccgggtt gaatcatatc gttgccgata taggttgaaa
    15961 attcgtaaag caccagacag agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg
    16021 aagctaattt attttgcatg caatttcttc gccaataata atcgcgcaga gtttaataaa
    16081 agcgcagcta acgagaaagc gaattttgta gctgaaacca cggttaagca cattcttaca
    16141 ttattgcgag tatagctaag ctttctttaa gttttattta acctatgccc gttacaatca
    16201 cccaccgtaa acaggccgct tgagggaaat aagacgatgc cgctttaccc agtttaacct
    16261 gcactttatt ctcaacgact tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa
    16321 gttatgctgg aaaatttgaa tctctctcta ttctctctta ttaacgcgac gccagactcg
    16381 gctccgtgga tgatctcgtt ggcgattttt attgctaaag atttgattac cgtggtgccg
    16441 ctgctggccg cggtactttg gttgtggggg cttacagcac aacggcaact ggtgataaaa
    16501 atcgctatcg cgctggcggt cagcctgttt gtgtcctgga cgatgggaca tctttttccg
    16561 cacgaccgac cctttgtcga aaatatcggc tataacttcc tgcatcatgc ggcggatgac
    16621 tcattcccaa gcgatcacgg tacggtgatt ttcacctttg cactggcatt tttatgctgg
    16681 catcgcctgt ggtccggctc acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc
    16741 gtttatcttg gcgtgcactg gccgctggat atgctcggtg gattgctggc aggtatgatt
    16801 ggctgcctta gcgctcagat tatctggcaa gcgatggggc ataaactcta tcaacgtctg
    16861 caatcgtggt atcgcttctg ttttgcatta ccgatccgca aaggctgggt gcgtgactga
    16921 tctctgctaa aaagtgtagt attgagcggc tcgcttcaat aactattcag agggattatg
    16981 gaaacacgtc gcgaagagcg tatcgggcag ctgctgcaag aattaaaacg cagcgataag
    17041 ttacatctta aagacgccgc cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat
    17101 ctgaacaacc acagtgcgcc cgtcgttttg ctcggcggct atattgttct ggaaccgcgc
    17161 agtgccagcc attacctgtt aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg
    17221 gcggcaaaac tggctgcgac actggtagaa cccgatcaga ctctcttttt tgactgtggc
    17281 accaccacgc cgtggattat tgaagcgatt gataatgaaa tcccttttac cgccgtttgt
    17341 tattcgctaa atacctttct ggcgctgaaa gagaaacccc attgccgcgc atttctgtgc
    17401 ggtggtgaat ttcacgccag caacgccatt ttcaaaccca tcgattttca gcaaacgttg
    17461 aataattttt gcccggatat cgctttttat tccgcggcgg gcgtgcatgt cagtaaaggc
    17521 gctacctgtt ttaatcttga agagttgccg gtaaaacact gggccatgtc gatggcgcaa
    17581 aagcatgtgc tggttgtcga ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt
    17641 gacctgaaac gctttgatat tgtggtgagt gattgttgcc cggaagatga gtatgtgaag
    17701 tacgcgcaga cgcaccgtat taagttgatg tattaatgac gtataaccgg atgacgtttc
    17761 gcgccatccg gttatcagaa gattaagaga accagctgcc gaaccactga tggaatttca
    17821 tcatcacgaa atcccacatc cgaccaaaga atccgccctc ttccacattt tccatcacga
    17881 tcagcggacg ctgctcaatg gatttaccgt taagctggaa atcaatggtc ccgacaacct
    17941 gacctttttt cagcggtgcg gtaagctgcg gttccgttaa cgtgtagctc gccttcaggt
    18001 ttttcagctg cccacgcggt atggtcactg agcccgcttc gcctgccccg agattcactt
    18061 cgctcttatc accaaaccag acgcgctgag tcacaaaggt ggcatcaggt ttaattggcg
    18121 tcacggtttc aaagaagcgg aaaccccagg tcaataattt ctcagactca ttaaaacgga
    18181 tacggtcagt tttcgccccc agcactacgg agattaaacg catatcgccc tgggtagccg
    18241 aagcaaccag attatatccc gcgcctgccg tggttcctgt cttcatgcca tcaacattca
    18301 gattgctgct ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact
    18361 ctttctcttt atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca
    18421 atgccatatc gcgcgcggta ctgaactgcc ccggcgcatc cagaccgtgc accgtctgga
    18481 aggtagtgtt ggtcagaccc agttttttgg cataaccatt catcaaacca ataaatgact
    18541 cctggctccc ggcaacgtaa tcggccagcg caatacaggc gtcattaccg gactggataa
    18601 tcacaccttt gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg
    18661 atgaaccacg cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat
    18721 cggtgagttt aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt
    18781 tagtcaggct cgcgggatcc agtttctcat ccgcgttgcc ttctgccagc actttaccgc
    18841 tggcgtaatc cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt
    18901 ccgccgcgaa tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac
    18961 gaaggagaga ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg
    19021 ttatatcgcc gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg
    19081 taagttggta aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc
    19141 atttttttgc cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag
    19201 cctgtggctg cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc
    19261 aactaacgta aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat
    19321 tctcgcgggc cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa
    19381 cgggctggtg ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc
    19441 cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc
    19501 acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac cagacactca gtaatgctca
    19561 tcgcgggatc ctgatgggat tagtcagaac accacccgaa gagcgcgatc aggccgccat
    19621 tgatgccagt tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa
    19681 agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat
    19741 ctacaatttg tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta
    19801 tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt
    19861 gcgtgggcta actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc
    19921 gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc
    19981 ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc
    20041 tataaataat ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct
    20101 cacagcgggc gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc
    20161 tttcgcttcc ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc
    20221 gtagtttttg cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt
    20281 cagccacagt gcattactcc acggattcat cgccattcct tgcggattac gaatgccata
    20341 agaccagatc tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc
    20401 gccctggtcg gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg
    20461 gcgctgattg ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg
    20521 cccgccaaaa tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc
    20581 ggtcactttt gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc
    20641 atcatcgcca acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc
    20701 taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag
    20761 cggcgcagat aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat
    20821 taacataccg tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg
    20881 cagtacttcg acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag
    20941 gggcacaagg aaaaagaatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc
    21001 gtagtcagtg gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga
    21061 cgctttctcc cccttagttt tcaggccttc tcaagcatgg cgtgcttctg caggctctgg
    21121 atactcagcg ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataaca
    21181 gtcgggcaat catggtgccc actcatcaaa catactgcgg ctgtcgctaa tacttcttca
    21241 gcatgatgaa gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta
    21301 tcgtgcagtt cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg
    21361 gacatgtatc cttttgcctt cctcagttgc acgtatgcgg cgaggtcaac gcgtgcgtcg
    21421 atcaaccggt tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct
    21481 ttccagcctt gttgcaatta aatagtatgg ctattttttg agcaaaatgc tgtccaggat
    21541 caattcttca cgccgaatta acaaaattta catttccttc gcctccgcca gcgaatgaga
    21601 ttgcgataca aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact
    21661 gaatattttt taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct
    21721 tggctgtcca aaaaaccttg ttgattcaga gcgtattctc accgaacttc gcactgaagg
    21781 ttatgacgtg gtaccgagct atgacgatgc ggacatggtg atcgtcaaca cctgcggctt
    21841 tattgacagc gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg
    21901 caaggtaatt gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc
    21961 gaaagtgctg gaaatcaccg ggcctcatag ctatgagcag gttctggagc acgttcatca
    22021 ctacgtgcca aaaccgaaac acaacccatt cctgagcctg gtgccagaac aaggtgtgaa
    22081 actgacgccg cgtcattatg cctatctgaa aatttctgaa ggctgtaatc accgctgcac
    22141 cttctgcatt attccgtcta tgcgcggcga cctggtgagc cgtccgattg gcgaagtgtt
    22201 aagtgaagcg aaacgtctgg tagatgcggg cgttaaagag attctggtga tctcgcagga
    22261 tacttccgcc tatggcgttg atgttaaaca tcgtactggc ttccacaacg gcgagccggt
    22321 aaaaaccagc atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct
    22381 gcactacgtt tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagaaggcaa
    22441 aatcctgccg tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat
    22501 gaagcgtccg ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg
    22561 cccggaactg accctacgct caacctttat tgtcggcttc cctggcgaga cggaagaaga
    22621 tttccagatg ctactcgact tcctgaaaga agcgcgtctg gatcgcgttg gctgctttaa
    22681 atacagcccg gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt
    22741 taaagaagaa cgctggaacc gtttcatgca gctgcagcag cagatttccg ccgagcgcct
    22801 gcaagagaaa gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc
    22861 gattggtcgc agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga
    22921 aaccaacgtt aagccgggtg atatcctgcg tgtgaaagtc gagcacgccg atgagtacga
    22981 tttgtggggt agccgggttt aattaattca ggccgggcaa aatcatttgt ccggtctgcg
    23041 aataatgatt actgccagct aatctgagcc ctgccgtttt tcttactctt atacagccgc
    23101 tcatccgcca gcattttcaa gcgttcgacg gtggtacatt catttgaatg cgctacaccg
    23161 atactggtac ttaaattaat gcttcccacc tggaaatgaa cgatttcttt tagaatcgat
    23221 agcaactctt ccagcgttcc acccgtataa atcaccgcaa attcgtcacc gccgaagcgg
    23281 tagattttac ctttatctac cgggatctgt gatttaagga ttttcaccac tttcatcaac
    23341 acttcatcgc ccagcaaatg accgaaggta tcgttaatgt ttttgaattt atcgacgtca
    23401 aacaggcaca aataaccgcc tttttccggc agcgctttta aatcttcgtc aaaggatttt
    23461 ctgcccattg ctttggtcag gtcgtccagg aacgccgagt tatacatact gtagagtttt
    23521 ttgacatcgg cagccgaaaa gaaaagcccg ccatccagta cgcgttcaaa atggttatta
    23581 atgatggctt ctttctccac attaccacga ttaatcactc tgcgtaccag atagaggcaa
    23641 ctggtacccg atatcactgc caccaatgcg gcaaagaaaa taatcaatgt acagacccgc
    23701 aaataaacat cttttttata cgcaaatacg tacaatccgg tcggcaaata ggcgaagtgc
    23761 tccatcgctt ccgaaacggt gaagatggcg tttttagcat tatattcatc accggtcacc
    23821 ggtttaccgt tcttaaatag agagaacatc aggttctcgc tgtccatcgc cagcgatttt
    23881 gacagtaaag aaaaacgctc cagaccaagc aaaaatatca gactacgatt gtgagttacc
    23941 ggatagaggt aatagacgaa ggttttaccg tcaaaagaga taaaccctgt atttttgaca
    24001 tgtcgatact tcacaatctg cataatatct gcacaataat ttttctcgaa ctcctttacc
    24061 tgaatacatt cgtcactgga aatcagataa ggaccgacat cgtacaaata agagggggaa
    24121 accgagtcgg cgtaagctgt gcgcaaacca accaacagct gttcctccgt gggatttagc
    24181 gtgttgataa gatcaattgc cacgctacgc cgatattcag caatatttgc caccgcaaaa
    24241 gcagtatcaa taacggactg tttcaggcta tacacccgtt cctttaccat ttgagtgtag
    24301 atcccgcagg caactgctga aatcatgata aaaatcagca gactgactgt aagtacgaac
    24361 ttattgattc tggacatacg taaactactc ttttactaat tttccacttt tatcccaggc
    24421 ggagaatggc atgggtttct ggtacagata cccctgagcc tgaacacatc ctaatttaat
    24481 taattcgcgc gcaacgtctt cgctctctac gccttccgca atcaccgggt tccggttata
    24541 gcgtgccaga tcgaaaataa tgcgcacaat ttttcccgca gtaccgaaaa tatcgccggt
    24601 aaattgcgag tccaccttta tgctgtctgg attaatttca taaaaacgtt tcaggtttga
    24661 caaaccggtg ccaaagtcat caatcgatat ccgtacccct tgtttacgca gttgttcaat
    24721 attgagataa aaatgttcat taattcgctc gatcacggtt tcagtgatct caacacagag
    24781 gctgtccgcc ttcaggccat tttctttcat gatggtggta aaacgctcat gaaagtgcgg
    24841 ttcgtttaac tgccagggcg aaacattaat atgcagtaag aaatcatcct tcagcgacgc
    24901 acgctgacta aaatgtctga attcacccat tgccgtttta ataatctgat aacccagctc
    24961 attgataaac ccgatatctt cagcaatggt aatgaacttc agcggtgaaa tgatcccccg
    25021 ctccgcagaa acccaacggc acaacgcctc cgcgcccaga atggcgcggg tgttaatatc
    25081 aacgatcggt tgatagacga ggaatagttc gccaatttcc attgcatggc gaatattacg
    25141 cgcaatccag atattctctt ctatttcgtt gagtttcgcg tcatcacaga aaataaactc
    25201 ggagaacata tggtcacgga tactggaaat cgccagtttc agtttttctg cgtaactcca
    25261 ggcgttttcc ccctgcaaag gttcacaaat ggcagactta tggatgcgca gattgcggta
    25321 gccaaagctg ctgtcaaaat cgtttaccat cgctatttta cgggtaaatg tctgtacatt
    25381 ttcccgacac agaagtaaat agagatcggc attgaatgtc acgatatcgc agcattgcgc
    25441 atatttctcg gcaattcgct gacacaattt cctgacgatt ttattgcgtt catcaacacc
    25501 aaacaccgct tccacggctt ccagaccaca cagtttgatg atccccacag ccagatactg
    25561 attattgttg gactcaataa tcgccatatt tttaataaaa ccgctttcct gatcgtaaga
    25621 gatcagtgag agcaatttat ttgaccgttc aatttcggca tctttcattt ccgcgatcga
    25681 atttttgatg ctgataatct ctttcgcatc gccttcgcga attttcagag cctcgatctg
    25741 atccgttgag tcgggcaaaa acgccagcgt attaaccagc tcggtcattt cagaaaattg
    25801 ccgacggaac acacgagtgg tcatgaaata taccaccagc aacacgataa taatgaacgc
    25861 aaacggtaga taaaaaagtt tatctgatgc gttcaataaa ttaccgccag gataataaaa
    25921 atagatttcg aacaatgcat tatttggcac tgctgatgtc tttgcaatat actccgtatc
    25981 gacagaatca ataatattat aattaccttc gctattataa ataatcagtt tttcacgagc
    26041 gagttgattc ttggcaatga tctcatgacc ttgtttcaaa aatagaactt catcattagc
    26101 gctgatttga tcaagatact cgttcagata ccgccagttt aaatcgacat gaattttatc
    26161 taccgctaac aaatcgtgat ctttgtcgtt gatacgtagc gtaaaagaga tacatttttt
    26221 acccggacga tcaaagcaat tgtatgccgg attccagctg ttgttagagt catctgaagc
    26281 caccgaccac ggtcgtttcg atgggttaaa atcggcaaaa ataacccgcc ctgactcgtc
    26341 gacaagcggt ccatttactt gttcctgatg ataaacaacg attttatcat tcgccctccg
    26401 tgaaaagaga taatatttat tatcatcaag taccagtccc accgagtcta aaaatggcat
    26461 catttccatt ttcagaatca cggcgcgttg aatctcctct gtatttttta ataaatacag
    26521 tgagtccttt aatgtcaggg c
//
LOCUS       DAJSKJ010000036        26361 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000036 DAJSKJ010000000
VERSION     DAJSKJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.036"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..182
                     /locus_tag="ON345_001314"
     CDS             <1..182
                     /locus_tag="ON345_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3071401.1"
                     /translation="ILQLSKSSIYSTKPKKTCLAQYTQHELDLVVAQLNNRPRKTLKF
                     KIPKEIIERGVALTD"
     gene            complement(189..1028)
                     /gene="ycjM"
                     /locus_tag="ON345_001315"
                     /pseudo
     CDS             complement(189..1028)
                     /gene="ycjM"
                     /locus_tag="ON345_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(1240..1554)
                     /gene="pspE"
                     /locus_tag="ON345_001316"
     CDS             complement(1240..1554)
                     /gene="pspE"
                     /locus_tag="ON345_001316"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR3071402.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1629..1850)
                     /gene="pspD"
                     /locus_tag="ON345_001317"
     CDS             complement(1629..1850)
                     /gene="pspD"
                     /locus_tag="ON345_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR3071403.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1859..2218)
                     /gene="pspC"
                     /locus_tag="ON345_001318"
     CDS             complement(1859..2218)
                     /gene="pspC"
                     /locus_tag="ON345_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR3071404.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2218..2442)
                     /gene="pspB"
                     /locus_tag="ON345_001319"
     CDS             complement(2218..2442)
                     /gene="pspB"
                     /locus_tag="ON345_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR3071405.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2496..3164)
                     /gene="pspA"
                     /locus_tag="ON345_001320"
     CDS             complement(2496..3164)
                     /gene="pspA"
                     /locus_tag="ON345_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR3071406.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3331..4308
                     /gene="pspF"
                     /locus_tag="ON345_001321"
     CDS             3331..4308
                     /gene="pspF"
                     /locus_tag="ON345_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR3071407.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4428..5693)
                     /gene="puuE"
                     /locus_tag="ON345_001322"
     CDS             complement(4428..5693)
                     /gene="puuE"
                     /locus_tag="ON345_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR3071408.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADGVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5731..7011)
                     /gene="puuB"
                     /locus_tag="ON345_001323"
     CDS             complement(5731..7011)
                     /gene="puuB"
                     /locus_tag="ON345_001323"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR3071409.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHNTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7013..8500)
                     /gene="puuC"
                     /locus_tag="ON345_001324"
     CDS             complement(7013..8500)
                     /gene="puuC"
                     /locus_tag="ON345_001324"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR3071410.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8775..9332)
                     /gene="puuR"
                     /locus_tag="ON345_001325"
     CDS             complement(8775..9332)
                     /gene="puuR"
                     /locus_tag="ON345_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR3071411.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9359..10123)
                     /gene="puuD"
                     /locus_tag="ON345_001326"
     CDS             complement(9359..10123)
                     /gene="puuD"
                     /locus_tag="ON345_001326"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR3071412.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10335..11753
                     /gene="puuA"
                     /locus_tag="ON345_001327"
     CDS             10335..11753
                     /gene="puuA"
                     /locus_tag="ON345_001327"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR3071413.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTESEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11902..12066
                     /locus_tag="ON345_001328"
     CDS             11902..12066
                     /locus_tag="ON345_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071414.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12056..13318
                     /locus_tag="ON345_001329"
     CDS             12056..13318
                     /locus_tag="ON345_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR3071415.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLY
                     VMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFT
                     FVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASL
                     GGAYLWYLIRRYRKVPLYEGDRTPVSET"
     gene            13452..13697
                     /locus_tag="ON345_001330"
     CDS             13452..13697
                     /locus_tag="ON345_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR3071416.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14010..15653
                     /gene="sapA"
                     /locus_tag="ON345_001331"
     CDS             14010..15653
                     /gene="sapA"
                     /locus_tag="ON345_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR3071417.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15650..16615
                     /gene="sapB"
                     /locus_tag="ON345_001332"
     CDS             15650..16615
                     /gene="sapB"
                     /locus_tag="ON345_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR3071418.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16602..17492
                     /gene="sapC"
                     /locus_tag="ON345_001333"
     CDS             16602..17492
                     /gene="sapC"
                     /locus_tag="ON345_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR3071419.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17492..18484
                     /gene="sapD"
                     /locus_tag="ON345_001334"
     CDS             17492..18484
                     /gene="sapD"
                     /locus_tag="ON345_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR3071420.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18486..19292
                     /gene="sapF"
                     /locus_tag="ON345_001335"
     CDS             18486..19292
                     /gene="sapF"
                     /locus_tag="ON345_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR3071421.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19360..19713
                     /locus_tag="ON345_001336"
     CDS             19360..19713
                     /locus_tag="ON345_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR3071422.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20081..20869
                     /gene="fabI"
                     /locus_tag="ON345_001337"
     CDS             20081..20869
                     /gene="fabI"
                     /locus_tag="ON345_001337"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR3071423.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21014..22141
                     /locus_tag="ON345_001338"
     CDS             21014..22141
                     /locus_tag="ON345_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR3071424.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22209..24143
                     /gene="rnb"
                     /locus_tag="ON345_001339"
     CDS             22209..24143
                     /gene="rnb"
                     /locus_tag="ON345_001339"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR3071425.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPHELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24221..24325)
                     /locus_tag="ON345_001340"
     CDS             complement(24221..24325)
                     /locus_tag="ON345_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071426.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            24379..>26361
                     /gene="pdeR"
                     /locus_tag="ON345_001341"
     CDS             24379..>26361
                     /gene="pdeR"
                     /locus_tag="ON345_001341"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR3071427.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         6077 a         6862 c         6821 g         6601 t
ORIGIN      
        1 tgatccttca actcagcaaa agttcgattt attcaacaaa gcctaaaaag acatgtcttg
       61 cccaatatac tcaacatgaa ctagatctgg ttgttgctca gctaaacaac agaccgagaa
      121 agacactgaa gttcaaaata ccgaaagaga taattgaaag gggtgttgca ttgacagatt
      181 gaatctacaa tgttgtcttt atgcggaaca ttggtctcgg taatgatcac tgtacctggc
      241 gcaacgttat caataatcga ccgtaacagt ttgataatca gatgtgtttt ttccagatgg
      301 atgcagcttg ttcccggctc tttccacata aagccaacgg catccaggcg gacatactca
      361 gcacctttcg caaggtaaca gagtaaaaca tccaccatcg ccagcaacac ttcagggcta
      421 cggtaattca ggtcaatttg atcgtcacta aaggtggtcc ataaatggcg cgttgaatga
      481 tcgcgcatct ggaatggcgt taataacggt aacgcacgcg ggcgagtgac ggcgctgaga
      541 tcggtttgcg ggtcaacggc aataaaaaaa tcttcaaaac ctggatgctg ttgtaaatag
      601 tttttaaacc attcactttt tgccgacata tggttgcaga caaaatcaaa cattaaatga
      661 ctgcattcac cgagttgctg aatatcctgc cactcccccg cttcactggc gacctgatga
      721 taatcaatta ccgaaaagcc atcatcagat gaccatggat aaaacggcaa caaatgaaca
      781 tgtgaaaaaa tgctttgcag ccattgatgg taaaactgat taaatgtggg taatggtttt
      841 aaatcattgc tgtgaaattg atcggcatag gtaattaaca cgacatcact ttcatcccag
      901 tgttttttac gtcgctgtgt aattaatcgt ttcgcactct caagacgcgt tacaagtttc
      961 tgtaaatgag ttgcggtaaa tgttccaccg tagatttcgt ccaggtaatc cgtaattttc
     1021 tgtttcatgt tgcctttccg tggtagcggt cccatgaaac gagagactac tccgcagaaa
     1081 ataaactgtc aattgagcac aagggtttta gtcacgcagg aaatcatttt ttaccaggga
     1141 aaaagcgtat ttgtgagctg acgcaaataa tctatgggga tgtaaataag gtgtgacaga
     1201 aaaaaaacgg cgcataagcg ccgctcatgg tgaattcttt taacctttga ccttcggcat
     1261 tgcgatgtct ttcaggccac cggcgttctc aacgtgggta tatcccatct cgctaaggat
     1321 ctcttttgct tgccctgact ggcgtccggc attgcaatac actttcacgg tgtcgttttt
     1381 atccggaacg gcggtggcaa tgcgctcttt cacttccttc aggggaatat tgatggcccc
     1441 ctgaacgtgc tcttgctgat actgctctgg aacacgaaca tcgatccagt gttcagcggc
     1501 gaaaacgggc agtgaaaaca ccagtgccag agcaagtaag ccttttttaa acatgtaagc
     1561 gtccttctat ggatgaaaat tgccatccat tatacgcctg agatttaaca atttttgcga
     1621 aactcccctc accttttata acgctgtgcc agtttattag cagcccgact taacagcggt
     1681 tccagcgcca cagccagcaa cattttcagc ggtcggcgag caactgattt tatcgcccag
     1741 cccgccacac ccgccgggcc atagcgcagt gcggtaagaa gtaccagctt gcctgctaat
     1801 ttgaaaccag gctttacctt ttgcccggcc tgttgccagc gagtattcat aactttcctc
     1861 acagttgacg gaaacggcta cgtaacgtga aagtatcgga agtgacataa cgttccatct
     1921 cgcgtaaacg cgtttcactt gccgccagtt cacggtcgac ttcatccagc aattcgctgc
     1981 tggaaggtag ctgctcacca aaggccatgt tgtccggcat tggatcaagc gcaaatgaca
     2041 aaatgatgta agcaaccagg gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca
     2101 ggatacgcac cagttttacc ggtacatcaa aatagttggc aatcccggcg cagacgccgc
     2161 ggaccatgcc ctgctgtgga atacgccata attttttatt gagattaatg cccgccatta
     2221 gcgatccctc cagttcggat gttctgcatc aagaatagat tccagcgcct gaatacgttc
     2281 gcgcatccgt tttgcttcat cagccagttg cgctaatcgc tgctgctcac tttgcgacaa
     2341 ttcactgcga ccagaacgat tgctgtaatg cagccataac cagatcggta aaacaaacag
     2401 cacaaaaatg gttaacggaa tagccagaaa tagcgcgctc ataagtactc cttagacgat
     2461 gagcggcggc gcgttcggac gccgcctgga tgttattatt gattgtcttg cttcattttg
     2521 gcttttaatt gtgccagttg ttcgctgatt gcatcatcgg ctttcagttc ggcaaactga
     2581 tcgtccagcg atttttgttt accgaagctg tggctttctg cttccgcttc catctggtca
     2641 atacgacgtt cgaaagattc gaaacgagcc attgcttcat ccagtttgcc actgtccagc
     2701 tgacgacgca catcgcgcga cgagtttgcc gcctgatgac gtaacatcaa tgcctgctgg
     2761 cgagcgcgtg tttcgctcaa tttgttttcc agctcaccga tctctttctt catgcgtgcc
     2821 agcgtgtcgt ccaccagcgt cacttcatgt tccaggctct taatcagatc ggtcagtttc
     2881 tgtttttcaa ttaacgctgc acgtgccaga tcctctctct ctttcagcag cgccagttcg
     2941 gctttttcct gccattcaac ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt
     3001 ttcttttctg ccaacgcgcg cgccgaagta gaacgtactt caaccagtgt atcttccatc
     3061 tcctggatca tcagacgaac cagtttctgt ggatcttccg ctttctctaa cagagcgttg
     3121 atgttggcat tcacgatgtc ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga
     3181 tttctgcgtt caggattgtc ctgctgaact gttaatacaa tttgcgtgcc aattttttat
     3241 ctttttgatt tataaagatc tgattgaaga atcaacagca acatgccagg atgagttagc
     3301 gaattacact aacaagtggc gaatttcatc atggcagaat acaaagataa tttacttggt
     3361 gaggcgaaca gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa
     3421 ccggtgctca tcatcggcga acgcggcacc ggtaaagagc tgattgccag ccgcctgcat
     3481 tatctctcct cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa
     3541 aatctgctgg attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa
     3601 cgtcatccag ggagatttga acgtgccgac ggcggtacgc tatttcttga tgaactcgct
     3661 acggcaccga tgatggtgca ggagaaatta ttgcgcgtga ttgagtacgg tgaactggag
     3721 cgcgttggcg gcagccagcc attgcaggtg aatgtacggt tggtatgcgc gacgaatgcc
     3781 gatctcccgg cgatggtcaa tgaaggcact tttcgcgctg acctgctcga ccgactggct
     3841 tttgatgttg tacaactgcc accactgcgc gagcgcgaaa gcgacataat gttgatggca
     3901 gaacactttg ccatccagat gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg
     3961 gagcgcgcca gagaaacatt gctgaattat cgttggccgg gaaatattcg tgaattgaaa
     4021 aacgtggtgg aacgttcagt gtatcgccac ggcaccagcg attatccgct tgatgacatc
     4081 attattgatc cctttaaacg gcgtccgcct gaagacgcta tcgccgtttc agaaaccacc
     4141 tcgcttccaa cactgccgct ggatttacgt gagtttcaga tgcagcagga aaaagagttg
     4201 ctgcaactca gtttgcaaca agggaaatat aaccagaagc gcgcggctga attactgggg
     4261 ttaacttatc atcagttccg cgcgttgttg aaaaagcacc agatttagcg aagttgagca
     4321 atacaacttg ccggatgcgg cgtgaatgct ttattcggca tacataatca atacgtcgtt
     4381 tgcaggcctg ataagcgcag cgcatcagac attattgcgt tgggctatta atcgctcagc
     4441 gcatcctgca aaattttcat tgccgcatcg aattgcgcat ccgggatggt cagcggatac
     4501 aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca ggagcagccc ctgcgccagc
     4561 gcgcgttgct ggattttctg tgcaatcgcc gctgacggct cgcccgtttg cggatcgtta
     4621 aactctgccg caatcatcga ccccaggccg cgtaccgcag caatggccgg aacgctttct
     4681 ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt
     4741 gattctttgt cgataatatt gagcaccgcg tgcgcggcag ccaccgccag cgggttcccg
     4801 gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc
     4861 acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca tcgtcattaa gtccggctta
     4921 tcggcgtaat gatccatggc aaacagttta ccggtacgcg caaagccgct ttgcactcca
     4981 tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc gaatggcggc aaccagctct
     5041 tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt cgaaaataat ggccgccacc
     5101 tgcttcgctt caatgtccga tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt
     5161 gaaatgccgt gtaaatctga cggataaggt acgtgataca ccgaaccagg gaacgggccg
     5221 aagccgattt tgtacggcgc aacttttccg gtcagcgcca tggtcatata tgtacgaccg
     5281 tggaagccgc cgctaaacgc aatcacgcca ggtcgtccgg tatgggcgcg agcaattttc
     5341 accgcgtttt ccaccgcttc cgcgccggta gtgaagaatg cggttttagc ctgcccgctc
     5401 actggggcaa gggcgttaat tttctccgcc agcgtgacgt agctttcgta cggcacaatc
     5461 tgatacgccg tgtgggtaaa ctgttgcaat tgctgctcca ccgccgcgac cagatcaggg
     5521 tggcgatgcc cggtattcag caccgcaatg cctgcggcga aatcgatgta ttcgttgccc
     5581 tctacgtcct tcagcgtagc gttttctgcc gactgggcga agaagttaca catcacgcca
     5641 accccgcgtg gggtagcaga aagacgacgc tgatggaatt cattgttgct catgtttcac
     5701 accttttgac tcttcactgt taatcggaaa ttaaacgccc agacgatcgc gcaggctgta
     5761 ataagccgcg cccatcgcgg taaatggcac gcgcagcgtg cgcccgccgg ggaacgggta
     5821 atgcggcaga ttggcgaagg cgtcgaagcg ttcggcgtca ccgcgcagca gttcagcaat
     5881 caaacgcccg gcaagatggg tacaggtgac gccgtggccg ctgtaaccct gcatgtaata
     5941 gatgttggta tcaaggcgac cgaactgcgg catgcgcgac aaggtcagca ggaagttgcc
     6001 cgtccagcgg taatcaattt tcacgccctt cagctgcggg aaggttttca gcagtttcgg
     6061 caccacaagg cgctcaacgt catccgggtc gcgcgcgccg tagaccacgc cgccgccgta
     6121 cagcaggcgg ttgtcggcgg taagacggta gtaatcaagc agatagttac agtcttccac
     6181 acagtagttt ttcgggatca gcgaacgggc taaatcttcc gacagccgtt cggtggtgat
     6241 cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc ggctctactt tatcgcccag
     6301 atacgcattc ccggcgacaa tcacatactt cgccgtcacc tggcctttgg cagtgcgcac
     6361 aacggctggc gtgttgtgct ggatctgcgt cacggcagaa agttcataca cgcgcccgcc
     6421 gttgaggcgg atggcgtccg cttcgccaat ggcgaggttt agcgggtgaa tatgcccacc
     6481 gctgtgatcc agcagcgcac cggtatagcg atcgctggct acttcacggc gaatggcgtt
     6541 ggcgtccagc aattccagct gtttattgcc gtagcgttcc cagttctctt tctgctcttc
     6601 aagtgtggcg agctgtttat cattcatcgc cacaaacagg ccgccggggc ggtagtcgca
     6661 gtcaatctga taacgtttga tacgttcgcg gatgatctca ccaccttcga acatcatgct
     6721 gccgagcata cgggcggtgt ccatgccgta gcttttttcg atcacgtcga tgtcgcggct
     6781 gtaggagttc acaagctgcc cgccattgcg cccgcttgcg ccaaagccga tgcgtgaggc
     6841 ttcgagaacc actacgtcaa agcccgcttc cgccagatgc agcgcggagg agagcccggt
     6901 atagccgccg ccaaccacgc aaacgtcgca ggtgattgac tcattcagcg tgtcgaatgg
     6961 cgcgtattta ttcgcactgg cggcgtaata actgctggta tgttcggtca tttcaggcct
     7021 ccaggcttat ccagatggtt ttcagttcgg tgaatttttc aagggcatgc agggatttgt
     7081 cgcgaccgtt gccgctctgc ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt
     7141 agttattgac gaagacggaa ccggctttca ggcggcggct catacggtgc gcgcgggaga
     7201 ggtcgcgcgt ccataccgcc gcgccaagtc cgtactggct gtcgttggca agctgtagcg
     7261 cctgttcttc tgatgtgaaa cgcgtgacca ccagcaccgg accgaaaatc tcttcgcggc
     7321 ttaaggacgc attcgggtct acatccacaa agatggtcgg gccgatggcg gcagccagcc
     7381 cggcgttacg gccatccagc agcagttgcc ctttgccttc gccctcacga ataaagctat
     7441 ggaccgagtc ggcgtgggcg cagtcgatta aagtgcccat ggtggttgcg gggtcaagcg
     7501 gatggcccgg ctgccagttt tgcgcctgct gttttaacag ggctaagaat tcatcggcga
     7561 tgctctcttc cagcaacagg cgtgttccgg cgatgcacac ctgaccctgg ttgtagaaga
     7621 tgcctgctgc ggtggagctc gccgcttttt gcagatccgg acaatcggcg aaaacgatgt
     7681 tggcgctttt gccgcccgct tccagccaga cgcgtttcat gttgctgtcg cccgcatctt
     7741 tcagcagctg tttcccggta cgggttgaac cggtaaaggc aatggcgtcg atatcgttat
     7801 gacgcgacag cgcctgcccg gcttcatgac caaaacccgt caccacgttc aacacaccat
     7861 ccggcaagcc tgcttctttc gccagccccg cgagacgaat cgcactgagc ggtgattttt
     7921 cagacggttt tagaatcacg ctgtttcccg ccgccagcgc cgggccgagt ttccagcaag
     7981 tcagcaacag cgggaagttc cacggcacga tggcggcaat cacgccgacc ggttcacgca
     8041 cgatcatcgc cagctcatgg ctactggtgg tcgccacttc gccatacact ttgtcgatcg
     8101 cttcggcgta ccagcgaatg gcgcgcgccg cgccgggaat atcatcacgc agactgtgac
     8161 gaatcggttt gccggtgtcg agagtttcca gcagtgccag ctcttcggcg tgggcttcca
     8221 ttaaatcggc gagtttattc agtaccgctt tacgtttagc cggagaagag agtgaccagt
     8281 cgccgcgttc aaatacgccg cgtgctgcgc tcatcgcacg gtcgatatcg acgctcttgc
     8341 cgcgggcaat tttcgccagc ggtgcctggg tgaccggatc aacggtttca aaggtttcat
     8401 tttccgccgc agcagtatat tcaccgttaa taaataagcg gttttcaatg gcgagactta
     8461 acgctttatc ctgccagtaa gccagatgat gaaaattcat tatgactcct gtttcacgtc
     8521 tatcagatat atgcagtttg tgaacgtcag ggcgcagaga tccccgggcc gtatgcccgt
     8581 ttatttctgg cgacattctt tatatacact tcatccttca agccgcctct gccttggctg
     8641 cgttcgctcc cccgaatcac ttacctgagt aagctcatcg ggattcgctc acttgccgcc
     8701 ttgatgcaac ttgaatgatt ttgtgtatag attgccgggc gcattgcgct tacccggctt
     8761 acagaacaaa aagattaaaa cgtggtgggc gtatgggcgc tgataattcg gcaaatacct
     8821 gccgacgtat tactgaaact gtgcgggatg ccggtattaa tggcataact ttgccccgcg
     8881 acgaggtggt aatcctgacc attaatcgtc agaacaattt caccttccag tactgtgcct
     8941 atttcctcac cctgatgctt aattctttcc ccagtggttg tgcccggctg gtacgtttca
     9001 aagatcatcg ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat tgacacaccc
     9061 tgactgccca tctcaattaa gtcgtcctga ttaatgacga cctgcggctc atcaggtttt
     9121 tccggctcgg aaaagaattc cgagagtgac agaccataca ccttcagcag cttttgcagc
     9181 gtactgatgg cagggctgac tttatcttgt tctatcgtac tgatagcact gtgagtcagc
     9241 ccggagagtt cggcggcacg acgttgtgaa agcccctgct gctggcggat ttccgacaag
     9301 cgttttcctg gcgccagtcc ctcatcactc atatttgcat ttccttaaac tgtagtggtc
     9361 agagtcgctg tttttcagcg atatggtgct gacaagcggt gataaagccc tcgaacaata
     9421 tacgcgaaag cgcgtactcg ctactgttcc attccgggtg ccactgtacg cccagcgcaa
     9481 aaggatgatt gatgacgcta accgcctcaa ctaagccatc cggcgagcga gcttcaacac
     9541 gcaaccgtgg gctaacgacc ttcgcccctt gtccatgtag agagtttacc caaaagttgc
     9601 tacattcagg taacaacgca gacagtaatc ccccctcttc aacctgaact tcatgcgacg
     9661 gtgcatattg ctgttccacc ggcagttcgg gatcttcccg atgttccagc aattcaggct
     9721 gttcgcacag cttgcgatgc aacgaaccac cggttgccac cactagttct tgtaaacccc
     9781 ggcagatggc gaaaatgggg atgcgccttt cgagtgcggc attaattatc gccatgctca
     9841 gaagatcacg cccgggatcg gcgtcaggct catcgccgtt ttcaccatat aggtgcggct
     9901 gcacattgct gggactacca ggaagataaa tgccatcgag tttcggcaaa agttgttcaa
     9961 gtaacgacgg ttccgccagc gcatgtggta gcgcaattgg caagccgcct gcgtggatga
    10021 tggcattcag gtacttttct tgcagagtct gggtcgcatg acccttaagc ctgttcctgc
    10081 acattacgac accgataacc ggattgttca ttatattttc catgctcaat ctcacaaagt
    10141 ggactaaatt atcgccatta ctgcgcttta aatgaccttt atgttcaata ttttttcaat
    10201 ctagcagtgg gttttcattt ttgcaaactc aatttaacat ttgacaaaca tttagtttgc
    10261 atacagattc gaatggtggt cattatattt tacgctttga taacgagcgg aaaacaaacc
    10321 aaaggcgaag aatcatggaa accaatatcg ttgaagtaga gaactttgtt cagcagtcag
    10381 aagagaggcg gggtagcgcc tttacgcagg aagtgaaacg ctacctggag cgctacccga
    10441 atacgcaata tgttgatgtt ttacttactg atttgaatgg ttgcttccgt ggaaaacgta
    10501 tccctgtttc aagcctgaag aagttggaga aagggtgtta tttcccggcc tcggtgtttg
    10561 caatggatat tctgggcaac gtagtagaag aagcgggtct gggtcaggaa atgggcgagc
    10621 cggatcgtac ttgtgttcct gttctcggtt ccttaacccc ttctgccgcc gatccagagt
    10681 ttatcggtca gatgctcctg accatggtcg atgaagatgg cgctcccttt gacgttgagc
    10741 cgcggaacgt tcttaaccgc ctgtggcagc agctacgcca gcgcggattg ttcccggttg
    10801 tagcggtaga gctggagttc tatttactgg atcgtcagcg cgacgctgaa gggtatctgc
    10861 aaccgccctg cgcgcccggc accgatgacc gcaatacaca aagccaggtt tactccgttg
    10921 ataacctcaa ccacttcgcc gacgtgctca atgatattga tgaactggcg cagttacagt
    10981 taattcccgc cgacggtgcg gtcgccgaag cctcgccggg tcagtttgaa atcaaccttt
    11041 accataccga taacgtgctg gaagcctgcg atgatgcgct ggcgctaaaa cgcctggtgc
    11101 gtctgatggc agaaaagcat aagatgcacg ccacttttat ggcgaagccg tatgaagagc
    11161 acgcgggcag cgggatgcat atccatatca gtatgcaaaa taatcgtggc gagaatgtgc
    11221 tttctgacac agaaagcgaa gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg
    11281 acctgatgcc gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc
    11341 agccgggaat gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc accgtcgccc
    11401 tgcgtattcc gtgcggcgac cgtcataatc accgcgtgga atatcgcgtg gcgggtgccg
    11461 atgccaaccc atatctggtg atggcagcga tttttgccgg tattttgcat ggccttgata
    11521 acgagctgcc gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa ggcttaccct
    11581 tcccgattcg ccagagcgat gccctgggtg agtttatcga gaatgatcac ctgcgccgct
    11641 atttaggcga acggttttgc catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt
    11701 ttgagcgcct catcacagaa acagaaattg agtggatgtt gaaaaacgcg taattcccca
    11761 tgcggggttg tttcatcggc ctgagcggag ccaggcctga catttgctgg taatccagac
    11821 atttcccgga tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta acgattaaga
    11881 ctgtgacgag gaaatgagat gatgccgatg attaaatccc cgcatggcga gggaggctgc
    11941 gtatgcgccc ctcctgctac tgactggact ccgccccctt tgcttccctt gctaaacagg
    12001 tttgatttca ggtcgacacg accgcaaacc ttattacgca ggggaggcag caattatggc
    12061 tattaattca ccactgaata ttgctgcgca acccggcaaa acccgtctgc gaaaatcact
    12121 gaaattgtgg caggtggtaa tgatgggtct ggcctatctc acgccgatga ccgtatttga
    12181 tacttttggt attgtctccg gcattagcga cggtcacgtt ccggcgtcct atttgctggc
    12241 gctggcgggc gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc agtttccgga
    12301 ggccggttcg gcctatacct acgcgcaaaa gtcgattaat ccgcacgtcg gatttatggt
    12361 cggctggtca tcactgctgg attatctctt tttgccaatg atcaacgttt tgttggcgaa
    12421 aatctatctc tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt
    12481 cgccatttta accgccgcga atctgaagag cgtcaacctg gtcgctaact tcaataccct
    12541 gtttgtactg gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg ttcagggact
    12601 gcataaagga gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc
    12661 gcacctgatc ccgattatta ccggtgcgac gattgtctgt ttctcgttcc tcggtttcga
    12721 tgcggtgacc acgctttcgg aagagacgcc ggacgccgca ctgcctgaaa ttgcgctgta
    12781 cgtcggcggc aagctctttc agtcgatttt cctctgcacc acgtttgtga acacattagc
    12841 gtctggcctg gcttcacatg ccagcgtgtc gcgtctgctg tatgtgatgg ggcgtgacaa
    12901 tgtgtttccg gagcgcgtgt ttggctatgt gcacccaaaa tggcggactc cggcactcaa
    12961 cgtcattatg gtcgggattg tcgcgctgtc ggcgctgttc tttgatttag tcaccgcgac
    13021 agcactgatt aacttcggtg ctctggtggc gtttaccttc gtgaatctgt cggtgtttaa
    13081 tcatttctgg cggcgtaaag gaatgaataa aagctggaag gatcacttcc actatttgct
    13141 gatgccgctg gttggcgcgc tgacggtggg tgtgctgtgg gttaacctag agtcaacgtc
    13201 actgacactc ggtctggtat gggcttcgtt aggcggcgca tatttgtggt atctgatccg
    13261 ccgctatcgc aaagtgccgc tgtacgaagg tgacagaacg ccggtgagcg aaacgtaata
    13321 gccggatgcg cggcgcatta tcctgcctga gggtaacgcg ccgcgccatt aactataaaa
    13381 agatgtatga cccggatcac gagtgctatc atccgccctt atgcttagcg cattaacccc
    13441 aggggcaaat aatgaaccac gatatcccgc taaaatattt tgatattgcc gatgagtacg
    13501 cgaccgaatg cgcagagcct gtcgcagagg ctgaacgcac accgctggcc cactacttcc
    13561 agttactgct cacccgtttg atgaacaatg aagagatcag cgaagaagcc caacatgaaa
    13621 tggctgctga agcggggatt aatcccgtac gcattgatga gatcgcagag tttttgaatc
    13681 aatggggtaa tgagtaatta tttaattgca gacgatcggt cagtatcgtt gtccggataa
    13741 agcgttcacg ccgcatccgg ctaacaatga cacgcactgc agcgtgcttt aatgggtttc
    13801 tcacgatcgt accgctctgc tcctcgctaa gttgttctgt gagttatagt tatagagaaa
    13861 tcacgggtac gaatgttgaa cagatggcga cgccacctgt tcaacgacca accacacggc
    13921 cctcattacg acccctgaac acaaacggta atggcaaatg tcattaaata ttagtaagat
    13981 agcctcttta cataaaatcc cttaatatta tgcgccaggt attatcgtct cttttggtga
    14041 ttgctggact tgtgagtggt caggcaatcg ccgcgcctga atcccccccg catgctgata
    14101 tccgcgacag cggttttgtc tattgcgtca gcgggcaagt caacaccttt aacccatcca
    14161 aagcgagcag tgggttaatt gtcgataccc ttgccgccca gttttatgat cgactgctgg
    14221 atgtcgatcc ctatacctat cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg
    14281 acaacggcgc gacctatcgc ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact
    14341 ggtttactcc cactcgtaaa atgaatgccg acgatgtggt gtttaccttc cagcgaattt
    14401 ttgaccgcaa caacccgtgg cataacgtca acggcagcaa cttcccctac ttcgacagcc
    14461 tgcaatttgc cgataacgtg aaaagcgtcc gcaaactgga taatcatacc gttgagttcc
    14521 gactcgccca gccggatgct tcttttttgt ggcacctcgc aacccattat gcttcggtca
    14581 tgtcggcaga atatgcccgg aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc
    14641 aaccggtcgg caccggtccg tatcagttgt cggaataccg cgccgggcaa tttattcgcc
    14701 tacaacgtca tgatgacttc tggcgcggta aaccgttaat gccgcaggta gtggtggatt
    14761 taggctccgg cggcaccgga cgtctgtcga aactcctgac cggggaatgc gacgttctgg
    14821 cctggcctgc tgccagccag ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc
    14881 tgcgtcctgg gatgaacgtc gcctatctgg catttaacac cgccaaaccg ccgctaaata
    14941 atcccgctgt ccgccatgca ctggcactgg cgattaataa ccagcgcctg atgcaatcca
    15001 tctattatgg tacggctgaa acggcggcct ctattttacc gcgcgcctcg tgggcctatg
    15061 acaacgaggc taaaattact gaatacaatc cggcgaaatc gcgcgaacag ttgaaggcgt
    15121 tggggctgga aaacttaacg ctgaaactgt gggtgcccac acgttcgcag gcgtggaacc
    15181 ccagtccact gaaaactgcc gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag
    15241 tggtgattgt gccggtagaa ggtcgctttc aggaggcgcg gttgatggat atgagccatg
    15301 atctgacgtt atccggttgg gcgacggaca gtaacgaccc ggacagtttc ttccgtcctt
    15361 tactgagctg cgcggcaatt cattcacaga ccaacctcgc ccactggtgc gatccgaaat
    15421 tcgacagcgt gttgcgtaag gcgctctcct cgcagcagct ggcggcgcgt attgaagcct
    15481 atgacgaagc gcagagtatt ctggcgcagg aattgcccat tttgccgctg gcgtcgtcat
    15541 tgcgtttgca ggcctatcgg tacgatatca aaggtctggt acttagcccg tttggtaacg
    15601 cctcctttgc tggggtgtat cgcgagaaac aggatgaggt gaaaaaacca tgattatctt
    15661 caccttacgc cgcattttgt tattgattgt caccttgttc ctgctgacct tcgttggctt
    15721 cagcttgagc tatttcaccc ctcatgcgcc gttacaaggc gcgtcactgt ggaatgcctg
    15781 ggtgttctgg tttaacggcc tgatccactg ggattttggt gtttccagca ttaacggtca
    15841 accgattgcc gaacagttaa aagaggtctt cccggcgacg atggaactgt gcatcctcgc
    15901 cttcggtttt gcgctgattg ttggcatccc cgtggggatg attgccggga ttacgcgcca
    15961 taagtggcag gataacttga tcaacgccat cgccttactg ggcttttcaa tcccggtgtt
    16021 ctggctggcg cttctgttga cgctgttttg ttcactcacg ctaggttggt tgcccgtttc
    16081 agggcgtttc gatctgctct acgaagtgaa accgattacc ggttttgcgt tgattgatgc
    16141 ctggctttcg gactcaccat ggcgggatga aatgatcatg agcgcaatcc gccatatgat
    16201 attgcccgtg atcaccctgt cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat
    16261 cagcactatc gaagtgtatg accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc
    16321 gcgctttact attttgcgtc gccacgttct gcataacgcg ttaccgccgg ttattcctcg
    16381 cctgggctta cagttttcca ccatgctgac gctggcgatg atcaccgaaa tggtttttag
    16441 ctggccgggt ttagggcgct ggttaattaa cgccatccgc cagcaggatt acgcagccat
    16501 ttccgccgga gtgatggtgt gtggctcact ggtcattatt gttaacgtga tttctgatat
    16561 tttgggtgcc atggctaacc ctctgaaaca taaggaatgg tatgccttac gatagcgtat
    16621 acagcgaaaa gcgcccgccg ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg
    16681 cctctgcgat ggtcggcctg tacggctgcg cggggctggc tgtactgtgt atttttggcg
    16741 gctggtttgc gccttacggc atcgaccagc aatttctcgg ttatcaatta ttgccgccgt
    16801 catggtcgcg ctatggcgaa gtttctttct tcctggggac tgacgacctc ggacgcgatg
    16861 ttttaagccg tttactgagc ggagctgcgc caaccgtggg tggcgcattt gtggtgacgc
    16921 ttgccgcgac gatctgtggc ctggtactgg gcaccttcgc cggggcgaca cacggcctcc
    16981 gctcagcggt gcttaaccat attctggata ccttactggc gatcccttcg ctgctgttgg
    17041 caattatcgt tgtagcgttt gccggaccga gtttgtccca cgctatgttt gctgtctggc
    17101 tggcattgct gccgcgtatg gtacgttcga tttacagcat ggtgcatgac gaactggaaa
    17161 aagagtacgt tatcgccgcc cgtctggatg gcgcatcaac gctgaatatt ctctggtttg
    17221 ctgtgatgcc aaacatcacc gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg
    17281 caattctcga tatcgccgcg ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg
    17341 aatggggagc gatgctcggt gatgcgctgg aactgattta tgtcgccccg tggactgtca
    17401 tgctgccagg tgcggcaatt atgattagcg tgttgttggt taacctcctt ggcgatggcg
    17461 ttcgtcgtgc gattattgcg ggggtggaat aatgccatta ctcgatattc gtaacctgac
    17521 cattgaattt aaaaccggtg atgagtgggt taaagccgtc gaccgggtaa gtatgacctt
    17581 aaccgaaggt gaaatccgcg gtcttgttgg tgaatccggt tccggcaaga gtttgattgc
    17641 gaaagcgatt tgtggggtga ataaagataa ctggcgtgtt actgctgacc gtatgcgttt
    17701 tgatgatatc gatttgctgc gtctctccgc acgcgaacgg cgcaaactgg ttggccataa
    17761 cgtgtcgatg attttccagg aaccgcagtc gtgtcttgac ccttcagaac gtgtgggtcg
    17821 ccagttgatg caaaacatcc cagcctggac ctataaaggc cgttggtggc agcgttttgg
    17881 ctggcgcaaa cgccgtgcca ttgaactgct gcaccgcgtg gggattaaag atcacaaaga
    17941 tgcgatgcgc agttttccct atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc
    18001 cattgcactg gcgaatcaac cgcgtctgct gattgctgac gaaccgacca actcaatgga
    18061 gccaacaacc caggcgcaaa tctttcgcct gctgacgcgt ctcaaccaaa acagcaatac
    18121 cactattttg cttatcagcc atgacttaca aatgcttagc caatgggcgg ataaaattaa
    18181 cgtgctttac tgcggtcaaa cagtggaaac cgcgccgagt aaggagttgg tgacgatgcc
    18241 acatcatcct tatacccagg cgctgatccg cgcgatacca gacttcggca gcgcgatgcc
    18301 gcataaaagt cgcctcaata cgctgcccgg cgctatccca ctgctggaac agttaccgat
    18361 tggttgtcgt ctggggccac gttgcccgta tgcacaacga gaatgcattg tgacgccacg
    18421 tttgacgggg gcgaaaaatc atctctatgc ctgtcatttc ccgctgaaca tggagaaaga
    18481 gtgagatgat cgaaacgctg cttgaagtgc gtaatttaag taaaacgttc cgctaccgga
    18541 ccggctggtt tcgtcgtcag accgtagaag cggtaaaacc cttgagcttt acgctacgtg
    18601 aaggccagac actggcgatt attggcgaga atggttcggg taaatccacg ctggcaaaaa
    18661 tgctggcggg aatgatagag cctaccagcg gcgagttatt gattgacgat catccactgc
    18721 attttggcga ttattccttc cgcagtcagc gtattcgcat gatttttcag gatccttcga
    18781 cctcgttgaa tccccgtcaa cgtatctcgc aaattctgga ttttccactg cgcctgaaca
    18841 ccgacctgga accggaacag cgtcgcaaac agattattga aacgatgcgt atggttgggc
    18901 tgctaccgga tcacgtcagt tactatccgc atatgttggc acccggacaa aaacagcgtc
    18961 tgggtctggc gcgcgcgctg atattgcgcc caaaagtcat tattgccgat gaagccctcg
    19021 cttcactgga tatgtcgatg cgttcgcagt tgattaatct gatgctggag ttacaggaaa
    19081 aacagggcat ttcgtatatt tatgttaccc agcatatcgg aatgatgaag cacattagcg
    19141 accaggtgct ggtgatgcat cagggcgagg ttgtcgagcg aggcagcacc gcggatgtgc
    19201 tggcatcgcc gctgcatgaa ctcaccaaac ggctgattgc cgggcacttt ggcgaagcat
    19261 tgacggcgga tgcgtggcgt aaagatcgct aattttctga atcctccttc cagattatcc
    19321 acctcattct gacaagagat taaatcttgt cagaatgagg tgatggataa aataaaatca
    19381 aatgcgcgtg atttaagacg caatctcact ttgcaggaac gaaagctctg gcgatatctt
    19441 cgcagccgac gttttggtga tttcaaattt cgccgtcaac atccggtggg gagctacatt
    19501 ctcgattttg cttgctgctc ggcgcgcgta gtcgttgagc tggatggtgg gcagcatgat
    19561 ttggcagttg cctatgatac caggcgcact agctggcttg agtcgcaggg ctggaccgtg
    19621 ctgcgtttct ggaataacga gattgattgt aatgaggagg cggtgctgga gattattctg
    19681 caggaactga accgccgatc accctcaccc tgaaagggcg agggggccga ccgagccgaa
    19741 tagctgttgt ggtgaaaaca tggagacagt gctggagaat attcggcaag gtctgaacca
    19801 tcccagccat cgccataaaa gggttagggg ctgtatgagc ctgtttgttg ctggggtaac
    19861 aatatttgca caatatggtc ccctcgcccc tctggggaga gggttagggt aaggggaaaa
    19921 gcgcccaccc tgccgcagcc tgctccggtc ggacctggca actatagcta ctcacagcca
    19981 ggttgattat aataaccgtt tatctgttcg tactgtttac taaaacgacg aatcgcctga
    20041 ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc tttccggtaa
    20101 gcgcattctg gtaaccggtg ttgccagcaa actgtccatc gcctacggta tcgctcaggc
    20161 gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac tgaaaggccg
    20221 cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg atgttgcaga
    20281 agatgccagc atcgacacca tgttcgctga actggggaaa gtttggccga aatttgacgg
    20341 tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact atgttaacgc
    20401 cgttacccgt gaaggcttca aaattgccca cgacatcagc tcctacagct tcgttgcaat
    20461 ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc tttcctacct
    20521 tggcgctgag cgcgctatcc cgaactacaa cgttatgggt ctggcaaaag cgtctctgga
    20581 agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat
    20641 ctctgctggt ccgatccgta ctctggcggc ttccggtatc aaagacttcc gcaaaatgct
    20701 ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag atgtgggtaa
    20761 ctccgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag tggtccacgt
    20821 tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa ttgaaataat cattctgttg
    20881 gtaaatattg ggcggcgttc tgccgcccgt tatctctgtt atacctttct gatatttgtt
    20941 atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt tcctttacga
    21001 ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt atcatgaaac
    21061 gcaatccagt actgcggagt atgacgttct gcctctggtc ccggaagccg caaaggtcag
    21121 cgatcccttt ctgctcgacg tgatccttga tgaagaaacg ctggctccct tcctttcatg
    21181 gctggtccct gcgcgcgttc ttgcagtgga attgttccct gaccagctta ccgtgacccg
    21241 ttcacagacc ttcaccgctt atgaacgctt gtctacggcc ctgacggttg ctcaggtttg
    21301 cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc ccgggcctga
    21361 ttcctccagg gaaagcaatc ataggttggc acaaatcacg caatatgccc gccaactggc
    21421 tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg tcggtcttac
    21481 tgcctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc aggcgcggac
    21541 cattgcgaca tttcaggctt atctcgggca cccggtacgc tggttacccg gtctggaaat
    21601 acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc gaagcagcta
    21661 tgaggtggaa aaactacctg aagagtacac aaaaagttca acagcagaac tttgccaact
    21721 ggccgaaaca ctctctcttc accctatttc actttccctt ctcgaaaggt tgttaaacag
    21781 tacacgggtt aatacacagc cggataataa gcttgcggcg ttgttatgcg cgcggataaa
    21841 tggcagtcct gcttgttttg ccgcctgtat ggattcatca aatgaatata aaaaaatcag
    21901 cgcccttgtg cgcaagggcg aaaatgaaat taaccaatgg gctgaccgtc attctgttga
    21961 gcacgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct ttagcgccgc
    22021 ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta atctgctggt
    22081 atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg agacatatta
    22141 accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat
    22201 agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa ctgcattccc
    22261 agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc ttcctggaag
    22321 tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg
    22381 accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag ccagaagaac
    22441 tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg
    22501 ccatcgttcc tgatcatcca ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc
    22561 tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt catccgctga
    22621 aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt gacgaccact
    22681 ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca ccagacggcg
    22741 tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgaccgcg ctggattttg
    22801 tcaccatcga cagtgccagc acagaagata tggatgacgc ccttttcgct aaggcgttgc
    22861 cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg attgctgaag
    22921 gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg cctggcttca
    22981 acatccctat gctgcctcac gagctttctg acgatctctg ctcactgcgc gccaatgaag
    23041 tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt gaagataata
    23101 tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac caggtttctg
    23161 actggctgga aaataccggt gactggcagc ctgaaagtga agcaattgcc gaacaagtcc
    23221 gtttgttagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac gcactggtgt
    23281 ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg
    23341 tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt gccgctaaca
    23401 tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac gtgcatatgg
    23461 gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac ggtctgcatg
    23521 tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt gaactggatg
    23581 cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt gctgaaatta
    23641 gcactgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc tggacttcgc
    23701 cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt atcaaaggcg
    23761 aaactgcgac gcgcccacag gatgaaatca ctgtccaaat ggccgagcgt cgccgtctca
    23821 accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg aaagacaaag
    23881 ccgggaccga cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc ggcatgcgtg
    23941 tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta cacgctatgc
    24001 gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt gaaacggttt
    24061 acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa acccgcagca
    24121 ttattgcgcg cccggttgcg taatctcctt tcacggccca ttcctcatgg atgggccgtt
    24181 tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact ttcgtttgaa
    24241 aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaaaca atgcgtttag
    24301 ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga ctccgctgtt
    24361 aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta caactttctc
    24421 ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgttttgcg cttttctacc
    24481 accgaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc tgctcgcatc
    24541 agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga tgaaagcgat
    24601 ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg caacgcgtct
    24661 gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc ctttgctgag
    24721 caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg gaatatccaa
    24781 cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt cattgggcaa
    24841 agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg caataaccgt
    24901 gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac atgtaaaggc
    24961 cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa aaacgagatt
    25021 tttttaatct gttccggcac cgacattacc gaagagcgcc gcgctcagga gcgactgcgt
    25081 attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat gcaggattta
    25141 atcgatcacg ctattaatca tgcagataac aataaagttg gggttgtgta tcttgatttg
    25201 gataatttca aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca gttattacgc
    25261 gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc gcgtccaggt
    25321 ggggatgagt ttctggtact ggcatccaac acctcacaaa gcgcgctgga agcaatggca
    25381 tcacgaattt tgacccgctt acggctcccc tttcgcattg gtttaattga agtttatacc
    25441 agctgttcag taggtattgc actctctccc gaacatggtt cagacagcac ggctattatt
    25501 cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca attttgcgtt
    25561 tttaccccag aaatgaatca acgggtattt gaatatctct ggctggatac caacttgcgt
    25621 aaagcactgg aaaacgatca gttggttatt cactatcaac cgaaaatcac ctggcgtggc
    25681 gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca
    25741 ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg
    25801 gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga
    25861 gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa
    25921 caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt
    25981 tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt
    26041 gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc
    26101 tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct
    26161 gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg
    26221 atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat
    26281 gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgttggtat
    26341 aaacgctatc tgaagcgcgc a
//
LOCUS       DAJSKJ010000037        26159 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000037 DAJSKJ010000000
VERSION     DAJSKJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.037"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(91..1341)
                     /gene="icd"
                     /locus_tag="ON345_001342"
     CDS             complement(91..1341)
                     /gene="icd"
                     /locus_tag="ON345_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR3071428.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1513..2166
                     /gene="rluE"
                     /locus_tag="ON345_001343"
     CDS             1513..2166
                     /gene="rluE"
                     /locus_tag="ON345_001343"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR3071429.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2176..2637
                     /gene="nudJ"
                     /locus_tag="ON345_001344"
     CDS             2176..2637
                     /gene="nudJ"
                     /locus_tag="ON345_001344"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR3071430.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2691..3797
                     /gene="mnmA"
                     /locus_tag="ON345_001345"
     CDS             2691..3797
                     /gene="mnmA"
                     /locus_tag="ON345_001345"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR3071431.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVSDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3833..4474
                     /gene="hflD"
                     /locus_tag="ON345_001346"
     CDS             3833..4474
                     /gene="hflD"
                     /locus_tag="ON345_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR3071432.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLRRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4478..5848
                     /gene="purB"
                     /locus_tag="ON345_001347"
     CDS             4478..5848
                     /gene="purB"
                     /locus_tag="ON345_001347"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR3071433.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6016..6687
                     /gene="phoP"
                     /locus_tag="ON345_001348"
     CDS             6016..6687
                     /gene="phoP"
                     /locus_tag="ON345_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR3071434.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEMMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6687..8147
                     /gene="phoQ"
                     /locus_tag="ON345_001349"
     CDS             6687..8147
                     /gene="phoQ"
                     /locus_tag="ON345_001349"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR3071435.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8223..9344
                     /gene="roxA"
                     /locus_tag="ON345_001350"
                     /gene_synonym="ycfD"
     CDS             8223..9344
                     /gene="roxA"
                     /locus_tag="ON345_001350"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR3071436.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLKLINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9393..10619)
                     /gene="pepT"
                     /locus_tag="ON345_001351"
     CDS             complement(9393..10619)
                     /gene="pepT"
                     /locus_tag="ON345_001351"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR3071437.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQHAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRR"
     gene            10869..12005
                     /gene="potA"
                     /locus_tag="ON345_001352"
     CDS             10869..12005
                     /gene="potA"
                     /locus_tag="ON345_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR3071438.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11989..12846
                     /gene="potB"
                     /locus_tag="ON345_001353"
     CDS             11989..12846
                     /gene="potB"
                     /locus_tag="ON345_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR3071439.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12843..13637
                     /gene="potC"
                     /locus_tag="ON345_001354"
     CDS             12843..13637
                     /gene="potC"
                     /locus_tag="ON345_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR3071440.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13634..14680
                     /gene="potD"
                     /locus_tag="ON345_001355"
     CDS             13634..14680
                     /gene="potD"
                     /locus_tag="ON345_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR3071441.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14836..15657)
                     /gene="cobB"
                     /locus_tag="ON345_001356"
     CDS             complement(14836..15657)
                     /gene="cobB"
                     /locus_tag="ON345_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR3071442.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15673..16584)
                     /gene="nagK"
                     /locus_tag="ON345_001357"
     CDS             complement(15673..16584)
                     /gene="nagK"
                     /locus_tag="ON345_001357"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR3071443.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITMQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16613..17857)
                     /gene="lolE"
                     /locus_tag="ON345_001358"
     CDS             complement(16613..17857)
                     /gene="lolE"
                     /locus_tag="ON345_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR3071444.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNNYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKEKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17857..18558)
                     /gene="lolD"
                     /locus_tag="ON345_001359"
     CDS             complement(17857..18558)
                     /gene="lolD"
                     /locus_tag="ON345_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR3071445.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18551..19750)
                     /gene="lolC"
                     /locus_tag="ON345_001360"
     CDS             complement(18551..19750)
                     /gene="lolC"
                     /locus_tag="ON345_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR3071446.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20012..21085
                     /locus_tag="ON345_001361"
     CDS             20012..21085
                     /locus_tag="ON345_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR3071447.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKELPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21213..24659
                     /gene="mfd"
                     /locus_tag="ON345_001362"
     CDS             21213..24659
                     /gene="mfd"
                     /locus_tag="ON345_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR3071448.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHTGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     HIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNARLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24803..25765
                     /gene="ldtC"
                     /locus_tag="ON345_001363"
     CDS             24803..25765
                     /gene="ldtC"
                     /locus_tag="ON345_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR3071449.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(25848..26105)
                     /gene="bhsA"
                     /locus_tag="ON345_001364"
     CDS             complement(25848..26105)
                     /gene="bhsA"
                     /locus_tag="ON345_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR3071450.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6343 a         6819 c         6666 g         6331 t
ORIGIN      
        1 aaatatgctc gatatgctat taataatacc tacataaata aaacaacggg cgtgttatac
       61 gcccgttgca atattaaaca catgtagtga ttacatgttc ttgatgatcg catcaccaaa
      121 ttctgaacat ttcagcagtt tagcgccttc catcagacgc tcgaagtcat aggttacggt
      181 cttcgcattg attgcgcctt ccataccttt aacaatcagg tctgcggctt cagtccagcc
      241 catatggcgt aacatcatct cagcggagag aataatagag ccagggttta ctttgtcctg
      301 accggcgtat ttcggcgcag taccgtgggt ggcttcaaac agggcgcatt cgtcaccgat
      361 gtttgctcca ggggcgatac cgataccgcc aacctgcgct gccagagcgt cagaaatgta
      421 gtcaccgttc aggttcatac aggcgataac atcatattca gccggacgca gcaggatctg
      481 ttgcaggaat gcatcagcaa tcacgtcttt aatgacgatc tctttgccgg tgttcgggtt
      541 tttaactttc agccacgggc cgccgtcgat cagttcaccg ccaaactctt cacgcgccag
      601 ctggtagccc cagtctttaa acgcgccttc ggtgaacttc atgatgttgc ctttgtgcac
      661 cagagtcaca gagtcacgat cgttagcaat tgcgtattcg atcgctgcac gaaccagacg
      721 tttggtgcct tcttccgaac acggcttaat accgatacca caatgttccg ggaagcgaat
      781 tttcttcaca cccatctctt cacgcaggaa tttaatcact ttctcggcgt cggcagagtc
      841 tgctttccat tcgatacccg cataaatgtc ttccgagttt tcacggaaga taaccatatc
      901 ggtcagttca gggtgtttaa ccgggcttgg agtgccctga tagtaacgta ccggacgcag
      961 gcagatgtag agatccagtt cctggcgcag ggcaacgttc agagagcgaa taccgccacc
     1021 aacaggagtg gtcagcggac ctttaatggc aacgcgatat tcacgaatca gatcaagggt
     1081 ttcagcaggc agccagacat cctgaccata aacctgtgtg gatttttcac cggtgtaaat
     1141 ttccatccag gagattttac gctcgccttt ataggctttc tcgactgcag cgtcgaccac
     1201 tttcagcatg gctggggtta catctacacc gattccatca ccttcaatgt aagggataat
     1261 cggattttca ggaacgttga gtttgccgtt ttgcagggtg atcttcttgc cttgtgccgg
     1321 aacaactact ttactttcca ttcacctctc cttcgagcgc tactggtttg ctcgtctgcc
     1381 accacgttgc atatgcgttt gcgtcctgcg atacggatgc tttagagcaa ttttttgtta
     1441 atgatttgta attggcttgt caatactacc ctattgtttg ccgtcatgaa agatgcgcgt
     1501 tattaggcta taatgcggca attcataatc tctgaaaata ccatgcaaaa aacttctttt
     1561 agaaatcacc aggttaagcg attcagctcg caacgttcta ccaggcgtaa acctgaaaac
     1621 cagcccacgc gtgtgatcct gttcaataaa ccctacgatg ttcttccgca gttcaccgat
     1681 gaagccggac gcaaaacatt aaaagaattc atcccggttc agggcgttta tgcagcaggt
     1741 cgtcttgacc gcgatagcga agggttactg gtgctgacca ataacggcgc gttgcaggcg
     1801 cgtttaaccc agccgggcaa acgcaccggg aaaatctatt atgtgcaggt ggaaggtatt
     1861 cccacacaag acgcacttga agccttgcgc aatggcgtaa ccttaaatga tggccctacc
     1921 ctgcccgccg gcgcggagct ggttgacgaa ccagcgtggt tatggccgcg gaatccacca
     1981 attcgtgaac gcaaaagtat tcccaccagc tggctgaaga tcaccttata tgaaggacgt
     2041 aatcgccagg tgcgccgcat gaccgcccat gttggtttcc ccacgctgcg actgattcgt
     2101 tatgcgatgg gtgattacac tttggataac cttgccaatg gcgaatggcg agaagcaaca
     2161 gaataaggat gcacaatgtt taaaccgcac gttaccgttg cttgcgtagt gcacgcagag
     2221 ggtaaatttt tagtcgttga agagacgatt aatggtaaag cgttatggaa ccaacctgcc
     2281 gggcatctgg aagccgatga aaccttagtg gaagctgccg cccgtgagct atgggaagaa
     2341 accggcatca gcgcgcaacc gcaacacttt attcgtatgc atcagtggat tgcgccagat
     2401 aaaacgccgt ttttgcgttt cctctttgcc attgagcttg agcaaatatg cccgacccag
     2461 cctcatgaca gcgatatcga ctgctgtcgt tgggtcagcg ccgaagaaat tttaaaggcg
     2521 tcaaatcttc gttcgccgct ggtggcggaa agtatccgtt gttatcaaag cgggcaacgc
     2581 tatccgctgg agatgattgg cgattttaac tggcctttta caaagggtgt catctaaaag
     2641 gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt gagtgatcca atgtctgaaa
     2701 ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga ttcctccgtt tctgcctggc
     2761 tgttgcaaca acagggatat caggttgaag gcctgtttat gaagaactgg gaagaagacg
     2821 acggtgagga atattgcaca gcggcagcgg atctggctga tgcccaggct gtctccgaca
     2881 agctcggcat tgaactgcac accgttaact ttgctgccga gtactgggac aatgtcttcg
     2941 aactgttcct tgccgaatat aaagccggtc gcacgccgaa tccggatatt ctgtgcaaca
     3001 aagagatcaa atttaaagct ttcctcgaat ttgccgccga agatttaggt gccgattata
     3061 tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg caagagccgc ctgctgcgtg
     3121 gtctggacag caataaagac cagagctact tcctttatac gctcagccat gagcagattg
     3181 cgcaaagcct gttcccggtc ggcgaactgg aaaaaccgca ggtgcgtaag attgctgaag
     3241 atcttggtct ggtcaccgcg aagaaaaaag actctaccgg catttgcttc attggcgaac
     3301 gtaaattccg cgagttcctg ggccgttatc tcccggcgca accgggcaaa atcattaccg
     3361 tcgatggcga tgaaattggt gagcaccagg ggctgatgta tcacactctc ggtcagcgta
     3421 aaggtctggg tatcggtggc accaaagaag gcaccgaaga accgtggtat gtggtggaca
     3481 aagacgtcga aaacaacatt ctggttgtcg ctcagggcca tgaacacccg cggctgatgt
     3541 ctgtcgggtt gattgcccag cagttgcact gggtcgatcg cgaaccattc accggcacta
     3601 tgcgttgcac ggtaaaaacc cgctatcgcc agaccgatat cccttgcacc gtcaaggcgc
     3661 tggacgatga tcgcattgaa gtgattttcg atgaaccggt tgccgccgtg acgccgggcc
     3721 agtctgccgt cttctataac ggtgaagtgt gcctcggtgg cggtattatt gagcagcgtc
     3781 tgccgctgcc ggtctgatta ttatctttac ttaaaacaag gaagcagtga acgtggcaaa
     3841 gaattactat gacatcaccc tcgccctggc cggtatttgt cagtcggcac gcctggtgca
     3901 acaactcgct caccaggggc attgtgatgc cgatgcgcta cacgtctcac tcaacagtat
     3961 tattgatatg aaccccagct cgacgctggc ggtttttggc ggtagcgaag ccaacctgcg
     4021 cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc agtcgccagg gcttaaacgc
     4081 cgaattaacc cgctacacac tcagcttgat ggtgcttgag cgcaaactct cctcagcgaa
     4141 aggcgcgctc gacactctgg gcaaccggat caacggcctg cgacgccagc tcgaacactt
     4201 cgatttacag tccgaaacgc tgatgagcgc gatggctgct atctatgttg atgtgatcag
     4261 cccgcttggc ccgcgcattc aggtcaccgg ttcccctgct gtactgcaaa gcccacaagt
     4321 gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc gccgccgtgc tctggcacca
     4381 ggtcggcggc ggacgtctgc aactgatgtt ttctcgtaat cgcctgacca ctcaggcaaa
     4441 acaaattctt gctcatttaa ccccggagtt gtgatctatg gaattatcct cactgaccgc
     4501 cgtttcccct gtcgatggac gctacggcga taaagtcagc gcgctgcgcg ggattttcag
     4561 cgaatatggt ttgctgaaat tccgtgtaca agttgaagta cgttggctgc aaaaactggc
     4621 cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc gacgcaatcg gttaccttga
     4681 tgcaattgtc gccaatttca gcgaagaaga tgccgcacgc atcaaaacca tcgagcgtac
     4741 cactaaccac gacgttaaag cggttgagta tttcctgaaa gaaaaagtgg cggagatccc
     4801 ggaactgcac gcggtttctg aattcatcca ctttgcctgt acttcggaag atatcaataa
     4861 cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa gtgatcctgc cgtactggcg
     4921 tcaactgatt gatggcatta aagatctcgc cgttcagtac cgcgatatcc cgctgctgtc
     4981 tcgtacccac ggtcagccag ccacgccgtc aaccatcggt aaagagatgg caaacgtcgc
     5041 gtaccgtatg gagcgccagt accgccagct taaccaggtg gagatcctcg gcaaaatcaa
     5101 cggtgcggtc ggtaactata acgcccacat cgccgcttac ccggaagttg actggcatca
     5161 gttcagcgaa gagttcgtca cctcgctggg tattcagtgg aacccgtaca ctacccagat
     5221 tgaaccgcac gactacattg ccgaactgtt tgattgcgtt gcgcgcttca acaccattct
     5281 gatcgacttt gaccgtgatg tctggggtta tatcgccctt aaccacttca aacagaaaac
     5341 cattgctggt gagattggtt cttccaccat gccgcataaa gttaacccga tcgacttcga
     5401 gaactccgaa gggaatctgg gcctttccaa cgcggtatta cagcatctgg caagcaaact
     5461 gccggtttcc cgctggcagc gtgacctgac cgactccacc gtgctgcgta acctcggcgt
     5521 gggtatcggt tatgcgctga ttgcgtatca atccaccctg aaaggcgtga gtaaactgga
     5581 agtgaaccgt gaccatctgc tggatgagct ggatcacaac tgggaagtgc tggctgagcc
     5641 aatccagaca gttatgcgtc gctatggcat cgaaaaaccg tacgagaaac tgaaagagct
     5701 gactcgtggt aagcgcgttg acgccgaagg catgaagcag tttatcgacg gtctggcgtt
     5761 gccagaagaa gagaaagccc gcctgaaagc gatgacgccg gcaaactaca ttggtcgcgc
     5821 catcaccatg gttgatgagc tgaaataaac tcgtatcagt gccggatggc gatgctgtcc
     5881 ggcctgctta ttaagattat ccgcttttta ttttttcact ttacctcccc tccccgctgg
     5941 tttatttaat gtttaccccc ataaccacat aatcgcgtta cactatttta ataattaaga
     6001 cagggagaaa taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccac
     6061 cttaaagttc agattcagga tgctggtcat caggtcgatg atgcagaaga tgccaaagaa
     6121 gccgattatt atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcca
     6181 gacgaggacg gtctgtcact gattcgccgc tggcgtagca atgatgtttc actgccgatt
     6241 ctggtattaa ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgctggtgct
     6301 gatgattacg tgactaaacc atttcatatt gaagagatga tggcgcgaat gcaggcatta
     6361 atgcggcgta atagcggtct ggcttcacag gtcatttcgc taccaccgtt tcaggttgat
     6421 ctctctcgcc gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac
     6481 accattatgg aaacgttgat acgcaataat ggcaaagtgg tgagcaaaga ttcgttaatg
     6541 ctccaactct atccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga
     6601 cgtctgcgca aaaaaattca ggcacaatat ccgcaagaag tgattactac cgttcgcggc
     6661 cagggctatt tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct
     6721 cactgcgggt acgttttctg ttggcaacgg ctgcggtagt actggtgctt tcgcttgcct
     6781 acggaatggt cgcgctgatc ggttatagcg tcagtttcga taaaactacg tttcggctgt
     6841 tacgtggcga gagcaatctg ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg
     6901 tcgagttacc cgaaaatatc gacaagcaaa gccccaccat gacgctaatt tatgatgaga
     6961 acgggcagct tttatgggcg caacgtgacg tgccctggct gatgaagatg atccagcctg
     7021 actggctgaa atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct
     7081 tgctgagtgg agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg
     7141 acgcggagat gacccactcg gtggcagtaa acgtctaccc ggcaacatcg cggatgccaa
     7201 aattaaccat tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct
     7261 ggagctggtt tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg
     7321 tcgccgcctg gtggagttta cgccccatcg aagccctagc aaaagaagtc cgcgaactgg
     7381 aagaacataa ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac
     7441 gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta cgacaaatac cgtacgacgc
     7501 tcaccgacct gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt
     7561 ctctgcgtag tgaaaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca
     7621 gccgcatttc acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat
     7681 tgctcagccg cgagctgcat ccggtcgccc cactgctgga caatctcacc tcagcgctga
     7741 acaaagtgta tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct
     7801 ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta
     7861 aatattgcct cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg
     7921 tggtcgagga tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg
     7981 gtcaacgggt tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa
     8041 tcaccgagca atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga
     8101 tggaggtgat ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtccatact
     8161 tcacgcatta cgttaagcat ccgttataat cggttgcaga taccagcctg tggatgctta
     8221 acatggaata ccaactcact cttaactggc ccgattttct tgaacgtcac tggcagaaac
     8281 gcccggtggt gttaaaacgc ggctttaata attttattga cccgatctct ccagacgagt
     8341 tggcgggtct ggcgatggaa agcgaagttg acagtcgact ggtcagtcac caggatggca
     8401 aatggcaggt cagccacggt ccgttcgaaa gctacgatca tctcggtgaa accaactggt
     8461 cattactggt acaggcagtg aaccactggc atgagccgac cgccgcgctg atgcgaccgt
     8521 tccgtgaact accggactgg cgtattgatg atctgatgat ttctttttct gtacctggcg
     8581 gcggcgtcgg cccgcatctc gatcagtacg acgtgtttat cattcagggt accggacgtc
     8641 gtcgctggcg agtgggcgaa aagctgcaaa tgaaacagca ctgcccacat ccggatctgt
     8701 tacaggtcga tccgttcgaa gccatcatcg atgaagagct ggagcctggt gatattcttt
     8761 atattccgcc aggattcccg catgaaggct acgcgctgga aaatgcgatg aactattccg
     8821 tgggctttcg cgcgccaaat acgcgggaac tgattagtgg atttgccgat tatgtgctgc
     8881 aacgtgaact gggcggcaac tactacagcg atcctgatgt tccacctcgc gctcatcctg
     8941 cggatgttct gcctcaagag atggataaac tgcgtgagat gatgctcaaa ttgatcaacc
     9001 agccggaaca ctttaagcaa tggtttggcg agtttatatc ccagtcacgt catgaactgg
     9061 atatcgcgcc gccggaaccg ccttatcagc cagatgaaat ctacgatgcg ctgaaacaag
     9121 gtgatgtgct ggtgcgcctg ggtggtctgc gcgtattgcg cattggcgac gacgtgtatg
     9181 ccaatggtga gaagatcgat tccccgcacc gtccggcact ggatgcactc gccagcaaca
     9241 ttgcgctgac tgcggagaat tttggcgatg cgctggaaga tccgtcattc ctcgcgatgc
     9301 tcgcggcgct ggtcaatagc gggtattggt tcttcgaagg gtaagtttgg atttaagccg
     9361 gatgcggcat cacatgccgc atcccttttc gcttacctcc gttgcgccgt taactcggca
     9421 atacggacga tcacctgcac cgctttttcc ataccttcca gagtcacaaa ctcatgctta
     9481 ccatgatagt tgtaaccgcc agtgaacaag ttcgggcacg gtaatcccat aaacgacaac
     9541 tgcgcgccgt cggtaccacc gcggatcggt ttcagttccg gttcaatatc gcagtcacgc
     9601 atcgcatgct gggcgatatc gagaatatgc ggatgctcaa ccactttctc gcgcatattg
     9661 tagtaactgt cttcaatcac cagttcaatg tagcaatcag gatgtaaccc tttgcccact
     9721 tttttggcga tctccatcat tttacgttta cgcgcttcaa actgtttacg atcgaaatca
     9781 cggatgatgt agtgcatatc ggcccgttca acggtgcctt tcatgctcgc caggtgatag
     9841 aaaccttcat agccttctgt catttccggg ctttcatccg ccggaacttc cgcatgaata
     9901 cgtgccgcca gcgacagcgc atttaccatc actcctttcg ccgtgcccgg atgaacattg
     9961 ttaccgacaa ttttgatatt gaccgatgcg gcgttgaagt tttcaaactc cagttcgcct
    10021 acgccaccac cgtcaacagt gtaagcccag cgggcatcga aggcatcaac atcaaaatgt
    10081 ttcgcccctt tgcccacttc ttcatccggg gtaaaggcga cgcgaatatc accatgcgga
    10141 atgtttttct gttgcaatac cgccagcgcg gtcatgattt ctgcaatacc ggctttgtca
    10201 tcggcaccta acaaggtttt accatcggta gtaatcagcg tctgacccag tagctgatgc
    10261 agcaccggga acataaccgg tgataaaact tcatcgccga tacccagcgc aatatcgcca
    10321 ccgcgatagt tttcaacaat ttgcggattc acatttttgc cgctgcaatc cggtgaggta
    10381 tccacatgag aaataaagcc aatcgccggg atatcgccag ggacgttagc cggtaacgtc
    10441 gccatcaaag tgcccttctc acttaaggtc acattgataa gccccatctc ttcgagctgc
    10501 tctttcagca gatgcaataa cttccattgg ccttccgtgc tgggaacctg tctcacccct
    10561 gcttttgatt gggtatccag agacacgtag ttcaaaaatc gctcaagtag tttatccatg
    10621 tagtcaccct cactttttgt gacaacatta ttaagaagca agaaaagaca aatattgcgt
    10681 caggtcactt ttacccctgc aagcgggaat atttatcagc attaccttaa tgaatataaa
    10741 gctaagccag taattcacaa caaagattgg cgattcaagc ggtttgccgt agaattaccg
    10801 ccctcattaa gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa gccatccgtt
    10861 gcgtttacat gggacagagt aaaaaattga ataaacaacc gagttcgctt tcaccgctgg
    10921 tgcaattggc gggaattcgc aaatgctttg atggtaaaga ggtcattccc cagctggatc
    10981 tgactatcaa caatggcgag ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa
    11041 ccgttcttcg cctgattgca ggtctggaaa ctgttgattc cggacgcatc atgctggata
    11101 acgaggacat cacccacgtt ccggcggaaa accgctatgt gaacactgtt ttccaaagct
    11161 acgcactttt cccccacatg accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa
    11221 aaacccccgc tgctgaaatt acgccccgcg tgatggaagc cctgcggatg gtgcagctgg
    11281 aaaccttcgc tcaacgcaaa ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca
    11341 ttgcccgcgc ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc
    11401 tggattacaa actgcgcaag caaatgcaga acgagctgaa agcgttacag cgtaagcttg
    11461 gcattacatt cgtcttcgtg actcacgacc aggaagaagc actcaccatg tcagacagga
    11521 ttgtggtgat gcgcgatggt cgcattgagc aagacggcac gccgcgtgaa atctacgaag
    11581 agccgaaaaa cctgtttgtt gccgggttca ttggcgaaat taatatgttt aacgccacgg
    11641 tgatcgagcg actcgacgag cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata
    11701 tctacgttaa cttcgccgtt gaaccggggc aaaaactgca tgttctgctg cgcccggaag
    11761 acttacgtgt tgaagagatt aacgacgaca accacgctga agggctgatt ggttacgttc
    11821 gtgagcgtaa ctacaaaggc atgacgctgg agtcggttgt tgaactggaa aatggcaaga
    11881 tggtgatggt cagcgaattc ttcaatgaag acgatcctga ctttgaccac tctctcgacc
    11941 aaaaaatggc cattaattgg gtagaaagct gggaggtcgt actggctgat gaagaacaca
    12001 agtaagttcc agaatgtagt gattgtcact attgtcggtt ggcttgtgtt gtttgtcttt
    12061 ctgcccaacc tgatgatcat tggcaccagc tttttgaccc gcgacgacgc cagtttcgtc
    12121 aaaatggtct ttacgctgga taactacacg cgtctgctcg atccgctcta ttttgaagtg
    12181 ctattgcact cgctgaatat ggcgctgatc gccaccctcg cctgcctggt gctgggctat
    12241 ccatttgcct ggtttctggc gaagctgcca cacaaggtgc gtccgctgct gctgtttctg
    12301 ctgattgttc cgttctggac caactcattg attcgtatct acgggctgaa aattttcctc
    12361 agcaccaaag gctatctcaa cgagtttttg ctctggctgg gcgttatcga cacaccaatc
    12421 cgtatcatgt tcacgcccag tgcggtgatt atcggtctgg tttacattct gctgccgttt
    12481 atggtgatgc cgctgtactc cagtatcgaa aaactggata agccgttgct tgaggcggcg
    12541 cgcgatctcg gtgccagcaa gttacagact tttatccgta tcattattcc actgacgatg
    12601 ccgggaatta ttgccggatg tctgctggtg atgctgccag cgatgggcct gttctatgta
    12661 tccgacctga tgggcggtgc gaaaaacctg ctgatcggta acgtcatcaa ggtacagttc
    12721 ctcaatattc gtgactggcc atttggtgca gccaccagca ttacgctgac tatcgtaatg
    12781 ggcctgatgt tgctggttta ctggcgcgct tctcgtttgc tgaataagaa ggtggaactc
    12841 gaatgatcgg tcgactgctt cgcggcggtt ttatgaccgc tatctacgcg tacctgtata
    12901 tcccaatcat tattttgatt gtgaactcct ttaacagctc gcgctttggc atcaactggc
    12961 agggttttac caccaaatgg tatagcctgc tgatgaacaa cgacagcctg ttacaggcag
    13021 cgcagcattc actaacaatg gcggtgtttt cggcgacgtt tgctacgctt atcggttcac
    13081 tgacggcagt tgcgctgtac cgttatcgct ttcgcggtaa gccgttcgtt agcggaatgc
    13141 tgtttgtggt aatgatgtca ccagatatcg tgatggcaat ttcgctgcta gtgctgttta
    13201 tgctgctggg aattcagctt ggcttctggt cgctgctgtt ctcgcatatc accttctgcc
    13261 tgccatttgt ggtggtgacg gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg
    13321 aagcggcgaa agatctcggt gccagtgaat ttaccattct gcgaaaaatc attctgccac
    13381 tggcaatgcc agcggtggcg gcgggctggg tgttaagctt taccctgtcg atggacgatg
    13441 tggtagtttc ttcgttcgtc accggaccaa gttatgaaat tctgccgtta aaaatttatt
    13501 cgatggtcaa agtcggcgta tcgccggaag ttaacgcgct ggcaaccata ttactggtgc
    13561 tgtcgctggt gatggttatt gccagccagc ttattgctcg tgataaaacg aaaggtaaca
    13621 caggggacgt taaatgaaaa aatggtcacg ccacctgctc gcggcgggtg ctctggcact
    13681 gggcatgagc gccgctcacg ccgatgacaa caacacgctg tatttctaca actggaccga
    13741 gtacgtgccg ccaggactgc ttgaacagtt caccaaagaa accggtatta aggttatcta
    13801 ttcgacttac gagtcgaacg aaaccatgta cgccaagctg aaaacgtaca aagacggtgc
    13861 gtatgacctg gtggttcctt caacctatta cgtcgataaa atgcgtaaag aagggatgat
    13921 tcagaagatc gacaagtcga agttaacgaa cttcagcaat ctcgatccag acatgctcaa
    13981 caagcctttt gacccgaata acgactactc cattccgtat atctggggtg cgacggcgat
    14041 tggtgttaac ggtgatgcgg tggatccgaa atctgtcacc agctgggccg atctgtggaa
    14101 gccagagtac aaaggcagcc tgctgttgac cgacgatgcc cgtgaagtgt tccagatggc
    14161 gctgcgtaag ctgggctact ccggtaacac caccgatccg aaagagattg aagctgcata
    14221 taacgagctg aaaaaactga tgccaaacgt cgcagcgttt aactccgata acccggctaa
    14281 cccgtacatg gaaggcgaag ttaacctcgg catgatctgg aacggttctg ctttcgttgc
    14341 acgccaggcg ggtacgccta ttgacgtggt gtggccgaaa gaaggcggca tattctggat
    14401 ggacagcctg gcgatcccgg caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa
    14461 cttcctgctg cgcccggatg tggcaaaaca ggttgctgaa actatcggtt atccaacgcc
    14521 aaaccttgcg gcgcgtaagc tgttaagtcc agaagtggcg aacgataaaa cgctctaccc
    14581 ggatgctgaa accattaaaa atggcgaatg gcagaatgac gttggcgcag ccagcagcat
    14641 ttatgaagag tattatcaga agctgaaagc aggacgttaa ttcagcaagc gggcggtggt
    14701 attaccgccc gttttttagt tagcaaggat gtggctaatg agtcaggaca gtaaagtttt
    14761 atcccgctct ttcgtcggta ttgtggtccg ctcgtaggcc tgataagcgt agcgcatcag
    14821 gcaatgcttc ctgctttata atcccttcag caacttttca acaaactcag gcaccacctg
    14881 gcttgccggg ccgtaatatt tctcggcaaa ttcattaccg acctgacttg gttcgagatt
    14941 cagttccacg gtgtgcgcgc catgcagttt cgcttcgtga acaaacccag ccgccggata
    15001 aacatgcccg gaagtaccaa tggcaatgaa aatatcagcc atcgacaacg ccatataaat
    15061 ttcatccatg ccgagtggca tttcgccaaa ccaaacgacg tgcgggcgca agggcgccgg
    15121 gaactggcaa caatggcatt tatcttctgg cgtaacgtct ccggtccagt cgagaacctg
    15181 accactttgc gaacaacgaa ctttcagcaa ttccccatgc atatgaatga cattggtatt
    15241 acctgcgcgt tcatgcaggt tgtctatatt ctgcgttacc agcaaaaagc gatcgccgag
    15301 ggcgtcttgc agtttagcca gcgcaagatg cgcggcgtta ggctgaattt ctggctgctg
    15361 cagctgtcga cgacgggcgt tataaaacgc ttgcaccagt tcaggatcgc gatcgaaacc
    15421 ttccggcgtt gccacatctt caacccgatg ttcttcccac aggccatctg cggcgcgaaa
    15481 ggtacgaata cctgattccg cagaaattcc tgcccctgtc agtacgagta ctcttggttt
    15541 ttccattgct tccggcacca ctttatctct gaaaaaaata cgctgacgca aacgctcgcg
    15601 caggcggcgt ttatttttac gaaaacgact taaccgatga ccccgacgcg acagcatagc
    15661 aacctctgtt gtttaatcgg ttagatgtag gaaggccgca ccacgcattc ctcccgcatc
    15721 accgtggcgc gcgcgttcaa tgcgcggaac acgagctaca ggtaagagat gacgaggcag
    15781 cctgtccgcc agttgcattg tgattgccgg gaaattcgat aagccaccac caatgacgac
    15841 caggtcaggg tcaacgatgg tcaggatatt tcccagacaa accgctaata aatccagata
    15901 acgctcaacg tgcgcccttg cctgctcatc gccttgatca taaagcgcaa taatttcggg
    15961 agcctgcaac ggttgatgat aatagtgttg ccagagccac gcaaagcccc gacctgatag
    16021 ataattttcg atacaaccaa tttgaccaca gccgcagcgg cgtaacggga aatccagccc
    16081 catcatggtt aacgcatcaa ccggcagacg catatggcca aactcgccgg taatgtagct
    16141 tttaccggta atcggtttac cgttgaaaat cagcccgccg ccaacgccgg tgccgagaat
    16201 taaccccatc accagtggat attgcgtaaa ttcatcatcc caggcttctg aaagggcaaa
    16261 acagttggca tcgttatcaa ggcgtacatc gcgatcaaga cgtgcgctca ggtcggcacg
    16321 cagcggttta ccgctggcag cggggacatt ggcggcatac agcgtaccat cttctgtttc
    16381 cggcattccc ggaataccga tgccaacaga acctttacag ccaaaacgtt ggtcagcttc
    16441 ggctaccagc tcgcatactg catctaaaaa tgcgtcatag ctgtcacgcg gtgtcggcac
    16501 ccgcttttcc cactgcaact gccgaccgct atcaaacacg ccaagtgcaa tttttgttcc
    16561 accaatatca aacccgtaat acattgccgc tccttgtttt aatgtactgc ctttactggc
    16621 cgctaaggac tcgcgcaggg tcaatattac tggcgcgccg cgccggatac caacttgcca
    16681 aaagactcag caacaatgct gtgaccagta cgtagaagac gtccagccag tgcaactccg
    16741 atggcaggaa gtcaataaaa tagatatcgc tggagaggaa ctgatgaccg atcagctttt
    16801 caatccgctc aataatcggg gtaagttgca gtgaaacaac tacgccgata atcacaccac
    16861 acaggctacc gaacagccct gccagcaatc cataccagac aaagatggcg cgaattaaac
    16921 catctttcgc ccccagcgtt cttaatactg cgatatcgcc actcttctct ttcaccgcca
    16981 tcactaaggt ggagacgatg ttgaaacagg ccacgccaat caccagtacc atcgccagat
    17041 acataatggc gcggatcatc tggatatcgc gatacatata gccgtaagta ccaatccaac
    17101 ttttaatata aacatagttg ttggtcactt cacccgcatc gcgtaccagc ttattggcgt
    17161 tgaaaacatc cgtcatttta agggcaatac ctgacacgct ggaacccata tcaagatatt
    17221 gttgggcatc cgccagcggg atcatggcaa aactgtgatc gagttgacca ctcaactgca
    17281 aaataccggc aatgtgcaaa cgcacacgtt ttggctgcat cagtttatgc tcgggattcg
    17341 agttggggat catgatcgac acccaatcgc cctgcttcac tttcagcgca tccgccacgc
    17401 ctttgccgat gataatttgc tgttcgcccg ctttaaaatt gcgccaggca tccccctgaa
    17461 caaacgaggg taatgcgctc agacgctgtt cctgttgcgg gttaacgccc ttcacctgaa
    17521 ttgcgcgcag attggcacca ctttctacca gtccggtgaa attgatatac ggcgcagcgg
    17581 cggcaatacc cggcactttt tgcacgttat ccagtgcttc ctgccagtta gtccatggct
    17641 gatttaccgc ctcaatttca ccgtgcggta ccacagcaag aatgcggtta ttcagttcgc
    17701 gttcaaagcc gttcatcgcg cttaagccga cgatcaatac cgccacgccg agggcaatgc
    17761 caatggtaga aatgacagag attagcgaca ccatgccgcc gcgtcgccgt ccgcgactaa
    17821 aacgcagtcc aatcaataac gataaaggca tcgccattac tccgccccca tcaggctcag
    17881 ttccgccgtc agacgtccat cacgcatttc cagttggcgg ctcatacgtt tcgccagttg
    17941 caggtcgtga gtaaccacca ggaaggcggt gccctgcaag cgattcaatt ccccaagcaa
    18001 ctggaagatg ctgtcggcgt tacgcgcatc gaggttaccg gtaggctcat ccgcaagtac
    18061 cagacgcgga ttattgacca gcgcacgggc aatcgccaca cgctggcgtt cgccgccaga
    18121 aagttcagat gggcggtgat tcgcacgatg ctccagcccc accgctttta acatctccag
    18181 cgcacggctg ttgatttcag cgggcttttt cttgccaatc agcagtggca tagccacgtt
    18241 ttccagggca gtaaaatccg gcagcaggtg gtgaaactga taaataaagc ccagcttctg
    18301 gttgcgcagt tctgctttcg ccgccgaaga cagtttgctc atcggttgac cgttaaagat
    18361 cacatcgccg gaggttggtg tatccagccc gcccagcagg tgcagcaagg tacttttacc
    18421 ggaaccagag ctaccgacga tcgccatcat ttcaccttcg cccacgctga aactgacatt
    18481 gtgcagtaca tcggtttgca cactgccttc ctgatagcgt ttgcacaggt tgtcgcattg
    18541 caacaggatc ttattcataa cgtaaagcct cagcgggttg tgtggcggca gcgcgccatg
    18601 aagggtaaag cgtagacagc agcgcgatag ccatcgccac cagcgcaata acaatgacct
    18661 gtaaaggttc gatagccacc ggcagcgccg cgccatcaag caggacgccg attatcggca
    18721 tcagattatt taactggctg gcaagcagtg cgccaagcgc cgcaccgagg atcgcaccga
    18781 taatcccggc gctggcccct tgcaccataa agaccatcat gatttgtcgc ggagttaagc
    18841 cttgcgtttg caggatcgcg acttcgccct gcttctccat taccatcagc cctagtgagg
    18901 taataatgtt aaacgccgca acggcgacaa tcaggctcag cagcaagccc atcatatttt
    18961 tttccatgcg tacggcctgg aacagctcgc ctttacggtc acgccagtcc tgccatttgc
    19021 tgccttcagg cagtttttgc tgacttaaag agtcaacttt cagcggctca tccagccaca
    19081 aacgccagcc ggtaatattg cctgccggat aacgcatcag acgcgaggca tcctcaatat
    19141 tcaccagcat ttcatagcca tcgacttcac tgttggcggc gaaagtgcca atcacattga
    19201 acaggcgctg gcttggaata cgccccatcg gcgtgaactg gctggcagat ggtaccatca
    19261 cgcggatttg atcaccgcga ttaacgccta gctgtgaggc aagttgttcg ccgaggatga
    19321 cattatattt ccccggctcg aggtcagttt gtttcacatt gaccagatac ggtgtaagtg
    19381 gatctttttg cgccgggtcg ataccgagca tcaccccgac cgccacgctg cgcgcgcttt
    19441 gcagtaccac atcaccggta gtaataggtg cgacgcgatt aacgccgtcc agtttgactg
    19501 ccgtttccgg gagttgctgc gggttaagag agccatgctc agaagagaga attgcctgtg
    19561 gcatcaggcc aaggatgttg ttttgcagct cgcgctcaaa gccgttcatc actgacaata
    19621 ctgtgaccag cgccatcacc ccgagggtaa tgccgatggt agaaagccag gagacgaaac
    19681 gaccgaagcg atccgctgca cgcccacgca tgtaacgcag gccaataaat agagcgacag
    19741 gttggtacat gaaatccgtc tggttgctgt agcaaagaca cgagtatata agcgaaagca
    19801 agatgggtaa acgtctgaac tgtaagtgat cgtcagggct aatctgaaaa gcccccgtaa
    19861 atcaatcggc taaattgcca tgttttatca tcaatcttgc cgcctgatct taatctgaaa
    19921 aatcatccaa tacagacaga tacccattac acctcggatc ataacccgca ggatcaaccg
    19981 catgtaaatt aaaaaagagc gtgttgtcct gatgaaacaa aaagagctat ggattaacca
    20041 gatcaaaggg ttatgtattt gtctggtggt gatttatcac tcggtcatta ccttttatcc
    20101 gcatctgacc acttttcagc atccgttatc ggaagtcctg agcaaatgct ggatctattt
    20161 caatctttac cttgccccct ttcgtatgcc ggtttttttc tttatatccg gctatttgat
    20221 tcgccgctat atcgacagcg taccgtgggg aaattgtctc gataaacgga tctggaacat
    20281 cttctgggtg ctggcgcttt ggggcgtggt gcagtggctg gcgttaagtg cgctgaacca
    20341 gtggctggca cctgagcgcg atttaagcaa tgcctccaat gccgcttatg ccgattccac
    20401 cggtgagttc ctgcacggga tgatcaccgc cagcaccagc ttgtggtatc tgtatgcgtt
    20461 aattgtctat ttcgtggtat gtaaaatttt tagccgtctg gcgctgccac tattcgcctt
    20521 gtttgtactg ctgagtgtgg cggttaattt cgttcccacg ccgtggtggg gaatgaacag
    20581 tgtgatccgc aatttgcttt attacagcct tggcgcatgg tttggcgcaa caataatgac
    20641 ctgtgttaaa gagttgccgt tgcgccgcca tctgctgatg gcttctttgc ttaccgttct
    20701 ggcggtcggt gcctggttgt ttactatctc gctgctgttg tcgctggtat cgattgtggt
    20761 gatcatgaag ctgttttatc agtacgagca acgattcggt atgcgctcca ccagcctgct
    20821 gaatgtgatt ggctccaaca ccattgctat ctacaccacc catcgcattc tggttgaaat
    20881 attcagctta actctgcttg cgcaaatgaa cgcagcacgc tggtcgccgc aagtcgaact
    20941 gacactcctg ctggtttatc cctttgtcag tttgttcatc tgtactgttg cgggcttgct
    21001 ggtaagaaaa ctttcacagc gtgcattcag cgatctgttg ttctccccgc cttcactgcc
    21061 cgctgccgtc agttactctc gttaaaccat cgccccagcc ggggcgattc aatttgctaa
    21121 accatgtcga ttacggataa tgaacagcat tgcttatcag agatatgccc ccatatgttg
    21181 aggcatatcc taacgagaat ctgacaaccg ttatgcctga acaatatcgt tatacgctgc
    21241 ccgtcaaagc gggtgagcag cgtctgctgg gcgagttaac cggcgcagcc tgtgcaacgc
    21301 tggtagcgga aattgccgaa cgtcacgccg gtccggtggt actcattgca ccagatatgc
    21361 aaaatgctct gcgtttgcat gatgaaatca gccagttcac cgatcagatg gtgatgaatc
    21421 tggcggactg ggaaactctt ccctacgaca gtttttcgcc tcatcaggac attatctcct
    21481 cgcgcctttc caccctttac cagctaccga cgatgcagcg tggcgtactg attgttccgg
    21541 tgaatacgct tatgcagcgc gtttgcccac acagttttct ccacggtcat gcgctggtga
    21601 tgaaaaaagg tcagcgcctg tcacgagatg cattacgaac ccaactggac agcgccggtt
    21661 atcgccatgt tgaccaggtg atggagcacg gcgaatacgc cacgcgcggc gcgttgctgg
    21721 atctcttccc gatggggagt gagctgcctt atcgtcttga tttctttgat gatgaaatcg
    21781 acagcctgcg ggtgtttgac gtcgacagcc agcgcacgct ggaggaagta gaagcgatca
    21841 atctgctgcc cgcgcacgaa tttccgaccg ataaagcggc aattgaactg ttccgcagcc
    21901 agtggcgcga taccttcgaa gtgaagcgcg atccggaaca tatttaccag caagtgagta
    21961 aaggcacatt acctgccggg atcgagtact ggcagccatt gttcttcagc gaaccactgc
    22021 cgccgctgtt cagttatttc cctgccaata ccttgctcgt gaatactggc gatctggaaa
    22081 ccagtgccga acgtttccag gctgacacgc tggcgcgttt tgagaatcgc ggcgtcgatc
    22141 cgatgcgccc gctgttgcca ccacaatcgc tctggctgcg ggtggacgag ctcttctcag
    22201 agctgaaaaa ctggccccgg gtgcagctaa aaactgaaca tttaccgaca aaagccgcga
    22261 atgccaattt aggtttccag aaactgccag acctggccgt tcaggcacaa caaaaagcgc
    22321 cgctggatgc gctgcgtaag ttcctcgaga ctttcgacgg tccggtggtg ttctcggtag
    22381 aaagtgaagg tcgccgtgaa gcgctgggtg aactgctcgc acgaattaaa attgctccgc
    22441 aacgcattat gcgtcttgat gaagccagcg accgtgggcg ttatctgatg attggcgctg
    22501 ccgaacatgg ttttgtcgat aaggtgcgta atctggcgct gatctgcgaa agcgatctgc
    22561 tcggtgaacg tgttgcccgc cgtcgtcagg attctcgccg caccatcaac cccgatacac
    22621 tgatccgtaa ccttgcggag ctgcatactg gtcagccggt ggtccatctg gagcacggtg
    22681 tcgggcgcta tgccggaatg accacgctcg aagcgggcgg cattactggc gagtatttga
    22741 tgctcaccta tgccaacgac gccaaactgt atgttccggt gtcgtcactg catctgatta
    22801 gccgttacgc aggtggcgcg gaagaaaacg ccccgctgca taaacttggc ggcgatgcgt
    22861 ggtcacgcgc gcggcagaaa gcggcggaaa aagtgcgtga tgtggcggcg gaattgctgg
    22921 atatctacgc gcaacgcgcc gccaaagagg gcttcgcgtt taaacacgat cgtgagcagt
    22981 atcagttgtt ctgcgacagc ttcccgtttg aaaccacgcc ggatcaggcg caggccatta
    23041 atgcggtact tagcgacatg tgtcagccgc tggcaatgga tcgtctggtg tgcggcgatg
    23101 ttggctttgg taaaacagaa gtggcgatgc gcgccgcttt cctggcagta gataaccaca
    23161 agcaggtggc ggtgttggtg cctaccaccc ttctcgcgca gcagcattac gacaacttcc
    23221 gcgaccgttt cgccaactgg ccggtacgta tcgaaatgat ctcccgtttc cgcagcgcca
    23281 aagagcagac gcaaatcctt gcggaagtgg cggaagggaa aatcgatatt ctgatcggta
    23341 cgcacaaact gctgcaaagt gacgtcaagt ttaaagattt aggcctgctg attgtcgatg
    23401 aagaacaccg cttcggggtg cgtcataaag agcatattaa agcgatgcgc gcgaacgtgg
    23461 atattctgac gcttactgca acgccgatcc cacgtacgct gaatatggca atgagcggaa
    23521 tgcgtgacct gtcgattatc gccacgccgc ccgcccgtcg tctggcagtt aaaacctttg
    23581 tccgtgagta tgacagcctg gtggtccggg aggcgatcct gcgtgaaatt ttgcgcggag
    23641 gacaggttta ttatctctac aatgatgtgg aaaacattca gaaagccgcc gaacggctgg
    23701 cagaactggt gccagaagcg cggatcgcca tcggtcacgg gcagatgcgc gagcgcgaac
    23761 tggaacgggt gatgaatgat ttccatcatc aacgtttcaa cgtgctggtt tgtacaacca
    23821 ttatcgaaac cgggatcgac atcccgacag ccaacaccat tatcattgaa cgcgcggatc
    23881 acttcggtct ggcgcagctg caccagttac gcggtcgcgt cggacgttcg catcatcagg
    23941 catatgcatg gttgctgaca ccgcatccaa aagcgatgac taccgatgcg caaaaacgtc
    24001 ttgaagcgat tgcctcgctg gaagatctcg gggcaggttt tgcgctggca acgcacgatc
    24061 tggagattcg cggcgcgggt gaactacttg gcgaagaaca aagcggctca atggaaacca
    24121 tcggtttctc gctgtatatg gagttgctgg aaaacgccgt cgatgcactg aaagccggac
    24181 gcgagccgtc gctggaagat ctcaccagcc agcaaacaga agtcgagctg cggatgccgt
    24241 cgctattgcc agatgatttc atccctgacg tgaatgcgcg tttgtcgttc tacaaacgta
    24301 ttgccagcgc caaaacggaa aacgaactgg aagagatcaa agtcgagctt atcgatcgct
    24361 tcggcctgct gccggatccg gcgcgtaccc tgctggatat tgcccgtctg cgccagcaag
    24421 cgcagaaact ggggatcagg aagctggaag gtaatgagaa aggcggggtg atcgaatttg
    24481 ccgagaagaa tcacgttaat ccggcctggt tgattggttt gctgcaaaaa cagccgcagc
    24541 attaccgcct tgatggtccg acgcgcctga aatttattca ggatttgagt gagcggaaaa
    24601 cacgtatcga atgggtacgc cagtttatgc gtgaactgga agagaacgcg atcgcttaat
    24661 ggtaaatcgg gggcgtttct gcgcccccat ctgcaacatt tacaaattct ttgcacttcc
    24721 ctgcactatc cgacactgtc accatccata attcaggctt atctgtttat tacaataacc
    24781 ttatatttat tatggatttt tggtgatgat caaaacgcgt ttttctcgct ggctaacgtt
    24841 ttttacgttc gccgctgccg tggcgctggc gctaccggca aaagccaaca cctggccgct
    24901 gccgccagcg ggcagtcgtc tggttggcga aaacaaattt catgtggtgg aaaatgacgg
    24961 tggttctctg gaagccatcg ccaaaaaata taacgtcggc tttctcgctc tgttacaggc
    25021 taaccccggc gttgatcctt acgtaccgcg cgcgggcagc gtgttaacga tcccgttgca
    25081 aaccctactt ccagacgcgc cgcgcgaagg cattgtgatc aacattgcgg agctgcgcct
    25141 ctattactac ccgccgggta aaaattcggt aaccgtgtat ccaataggta ttggtcagtt
    25201 aggtggtgac acgctgacac cgacaatggt gaccaccgtt tcagacaaac gtgcaaaccc
    25261 aacctggacg ccaacggcaa acatccgcgc ccgttataaa gcacagggaa ttgagttgcc
    25321 tgcggtagtg ccggctggac cggataaccc aatgggccat catgcgattc gtctggcggc
    25381 ctatggcggc gtttatttgc ttcatggtac gaacgccgat ttcggcattg gcatgcgggt
    25441 aagttctggc tgtattcgtc tgcgggatga cgatatcaaa acactcttta gccaggtcac
    25501 cccaggcacc aaagtgaata tcatcaacac tccgataaaa gtctctgccg aaccaaacgg
    25561 tgcgcgtctg gttgaagtac atcagccgct gtcagagaag attgatgacg atccgcagct
    25621 gctgccaatt acgctgaata gcgcaatgca atcatttaaa gatgcagcac aaactgacgc
    25681 tgaagtgatg caacatgtga tggatgtccg ttccgggatg ccggtggatg tccgccgtca
    25741 tcaagtgagc ccacaaactc tgtaaatcct ggataaaaaa aatccccgcg acacggcatg
    25801 tcgcggggaa aaaatcagca gtagcaggca ttaatgaggg ttaatgctta tttataaatt
    25861 actgctgttc catggagggt attcggaccg gttacagaag taatacggaa agattttgcg
    25921 cccatctcat ccgctttttg cgccagctgc tcttccagcg atcccagatt tgtccccgcg
    25981 ttagcactga ttgtaccgac tttttgttgg ccttctggcg ttgactgaac ttcgacagcc
    26041 gcaaagctgg caaatgacat ggagcttaaa atcgccgcag cgatgagggt ttttacgttt
    26101 ttcataatag tggccttatg cagatgaatg acgtcgcaag caattactta acgatcgat
//
LOCUS       DAJSKJ010000038        26137 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000038 DAJSKJ010000000
VERSION     DAJSKJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.038"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            200..394
                     /gene="bfd"
                     /locus_tag="ON345_001365"
     CDS             200..394
                     /gene="bfd"
                     /locus_tag="ON345_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR3071451.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            467..943
                     /gene="bfr"
                     /locus_tag="ON345_001366"
     CDS             467..943
                     /gene="bfr"
                     /locus_tag="ON345_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR3071452.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(940..1407)
                     /locus_tag="ON345_001367"
     CDS             complement(940..1407)
                     /locus_tag="ON345_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR3071453.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1786..2097
                     /gene="rpsJ"
                     /locus_tag="ON345_001368"
     CDS             1786..2097
                     /gene="rpsJ"
                     /locus_tag="ON345_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR3071454.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2130..2759
                     /gene="rplC"
                     /locus_tag="ON345_001369"
     CDS             2130..2759
                     /gene="rplC"
                     /locus_tag="ON345_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR3071455.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2770..3375
                     /gene="rplD"
                     /locus_tag="ON345_001370"
     CDS             2770..3375
                     /gene="rplD"
                     /locus_tag="ON345_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR3071456.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3372..3674
                     /gene="rplW"
                     /locus_tag="ON345_001371"
     CDS             3372..3674
                     /gene="rplW"
                     /locus_tag="ON345_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR3071457.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3692..4513
                     /gene="rplB"
                     /locus_tag="ON345_001372"
     CDS             3692..4513
                     /gene="rplB"
                     /locus_tag="ON345_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR3071458.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4530..4808
                     /gene="rpsS"
                     /locus_tag="ON345_001373"
     CDS             4530..4808
                     /gene="rpsS"
                     /locus_tag="ON345_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR3071459.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4823..5155
                     /gene="rplV"
                     /locus_tag="ON345_001374"
     CDS             4823..5155
                     /gene="rplV"
                     /locus_tag="ON345_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR3071460.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5173..5874
                     /gene="rpsC"
                     /locus_tag="ON345_001375"
     CDS             5173..5874
                     /gene="rpsC"
                     /locus_tag="ON345_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR3071461.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5887..6297
                     /gene="rplP"
                     /locus_tag="ON345_001376"
     CDS             5887..6297
                     /gene="rplP"
                     /locus_tag="ON345_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR3071462.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6297..6488
                     /gene="rpmC"
                     /locus_tag="ON345_001377"
     CDS             6297..6488
                     /gene="rpmC"
                     /locus_tag="ON345_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR3071463.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6488..6742
                     /gene="rpsQ"
                     /locus_tag="ON345_001378"
     CDS             6488..6742
                     /gene="rpsQ"
                     /locus_tag="ON345_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR3071464.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6907..7278
                     /gene="rplN"
                     /locus_tag="ON345_001379"
     CDS             6907..7278
                     /gene="rplN"
                     /locus_tag="ON345_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR3071465.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7289..7603
                     /gene="rplX"
                     /locus_tag="ON345_001380"
     CDS             7289..7603
                     /gene="rplX"
                     /locus_tag="ON345_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR3071466.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7618..8157
                     /gene="rplE"
                     /locus_tag="ON345_001381"
     CDS             7618..8157
                     /gene="rplE"
                     /locus_tag="ON345_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR3071467.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8172..8477
                     /gene="rpsN"
                     /locus_tag="ON345_001382"
     CDS             8172..8477
                     /gene="rpsN"
                     /locus_tag="ON345_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3071468.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8511..8903
                     /gene="rpsH"
                     /locus_tag="ON345_001383"
     CDS             8511..8903
                     /gene="rpsH"
                     /locus_tag="ON345_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR3071469.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8916..9449
                     /gene="rplF"
                     /locus_tag="ON345_001384"
     CDS             8916..9449
                     /gene="rplF"
                     /locus_tag="ON345_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR3071470.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9459..9812
                     /gene="rplR"
                     /locus_tag="ON345_001385"
     CDS             9459..9812
                     /gene="rplR"
                     /locus_tag="ON345_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR3071471.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9827..10330
                     /gene="rpsE"
                     /locus_tag="ON345_001386"
     CDS             9827..10330
                     /gene="rpsE"
                     /locus_tag="ON345_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR3071472.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10334..10513
                     /gene="rpmD"
                     /locus_tag="ON345_001387"
     CDS             10334..10513
                     /gene="rpmD"
                     /locus_tag="ON345_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR3071473.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10517..10951
                     /gene="rplO"
                     /locus_tag="ON345_001388"
     CDS             10517..10951
                     /gene="rplO"
                     /locus_tag="ON345_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR3071474.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10959..12290
                     /gene="secY"
                     /locus_tag="ON345_001389"
     CDS             10959..12290
                     /gene="secY"
                     /locus_tag="ON345_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR3071475.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12322..12438
                     /gene="rpmJ"
                     /locus_tag="ON345_001390"
     CDS             12322..12438
                     /gene="rpmJ"
                     /locus_tag="ON345_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR3071476.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12585..12941
                     /gene="rpsM"
                     /locus_tag="ON345_001391"
     CDS             12585..12941
                     /gene="rpsM"
                     /locus_tag="ON345_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR3071477.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12958..13347
                     /gene="rpsK"
                     /locus_tag="ON345_001392"
     CDS             12958..13347
                     /gene="rpsK"
                     /locus_tag="ON345_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR3071478.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13381..14001
                     /gene="rpsD"
                     /locus_tag="ON345_001393"
     CDS             13381..14001
                     /gene="rpsD"
                     /locus_tag="ON345_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR3071479.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14027..15016
                     /gene="rpoA"
                     /locus_tag="ON345_001394"
     CDS             14027..15016
                     /gene="rpoA"
                     /locus_tag="ON345_001394"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR3071480.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15057..15440
                     /gene="rplQ"
                     /locus_tag="ON345_001395"
     CDS             15057..15440
                     /gene="rplQ"
                     /locus_tag="ON345_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR3071481.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15547..15915
                     /locus_tag="ON345_001396"
     CDS             15547..15915
                     /locus_tag="ON345_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR3071482.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVLPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15926..16351
                     /gene="zntR"
                     /locus_tag="ON345_001397"
     CDS             15926..16351
                     /gene="zntR"
                     /locus_tag="ON345_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR3071483.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16407..16625
                     /gene="arfA"
                     /locus_tag="ON345_001398"
     CDS             16407..16625
                     /gene="arfA"
                     /locus_tag="ON345_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR3071484.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16622..17032)
                     /gene="mscL"
                     /locus_tag="ON345_001399"
     CDS             complement(16622..17032)
                     /gene="mscL"
                     /locus_tag="ON345_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR3071485.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17162..18538)
                     /gene="trkA"
                     /locus_tag="ON345_001400"
     CDS             complement(17162..18538)
                     /gene="trkA"
                     /locus_tag="ON345_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR3071486.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18560..19849)
                     /gene="rsmB"
                     /locus_tag="ON345_001401"
     CDS             complement(18560..19849)
                     /gene="rsmB"
                     /locus_tag="ON345_001401"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR3071487.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19895..20842)
                     /gene="fmt"
                     /locus_tag="ON345_001402"
     CDS             complement(19895..20842)
                     /gene="fmt"
                     /locus_tag="ON345_001402"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR3071488.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20857..21366)
                     /gene="def"
                     /locus_tag="ON345_001403"
     CDS             complement(20857..21366)
                     /gene="def"
                     /locus_tag="ON345_001403"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3071489.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21496..22620
                     /gene="dprA"
                     /locus_tag="ON345_001404"
     CDS             21496..22620
                     /gene="dprA"
                     /locus_tag="ON345_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR3071490.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22592..23065
                     /locus_tag="ON345_001405"
     CDS             22592..23065
                     /locus_tag="ON345_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR3071491.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23094..23636
                     /locus_tag="ON345_001406"
     CDS             23094..23636
                     /locus_tag="ON345_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR3071492.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23641..24213
                     /gene="tsaC"
                     /locus_tag="ON345_001407"
     CDS             23641..24213
                     /gene="tsaC"
                     /locus_tag="ON345_001407"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR3071493.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24218..25036
                     /gene="aroE"
                     /locus_tag="ON345_001408"
     CDS             24218..25036
                     /gene="aroE"
                     /locus_tag="ON345_001408"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR3071494.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25033..25290
                     /locus_tag="ON345_001409"
     CDS             25033..25290
                     /locus_tag="ON345_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR3071495.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25266..25820)
                     /locus_tag="ON345_001410"
     CDS             complement(25266..25820)
                     /locus_tag="ON345_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR3071496.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6712 a         6198 c         6783 g         6444 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 cccttttgct gcccgtaatt tgagctcgaa atatttagtc gtaattttgc tcgctgaact
      121 atggcgcaaa taaaactgtg ctattgtaat cataaccatt ctcatttaca ctttgcgcag
      181 aatttgaacg ggagcaatca tgtacgtttg tttgtgtaat ggtgtaagtg acaaaaaaat
      241 tcgccaggca gtccgtcagt ttcatccgca atctttccaa caattgcgta agtttattcc
      301 tgtgggaaat caatgcggta agtgcattcg cgccgcgcgc gaagtgatgc aggacgagtt
      361 gatgcagatg ccagaattta aagagattgc ctgaagacca cgcttatttt ttgacatccc
      421 gctagcccca tctacgctct aatagtggaa gcggagggac tataaaatga aaggtgatgt
      481 taaaatcata aattatctca ataaactatt gggaaatgag cttgtcgcaa tcaatcagta
      541 ttttctccat gcccggatgt ttaaaaactg gggcctgatg cgtctcaatg acgtcgaata
      601 ccatgaatcc atcgatgaga tgaaacacgc tgataaatat atcgagcgta ttttgttttt
      661 agaaggaatc cccaacttac aggatttagg caaactgggg attggtgaag atgtcgagga
      721 gatgctgcaa tccgacctgc gattagaact ggaaggcgcg aaggatctcc gtgaggctat
      781 cgcgtatgcc gacagcgttc atgactatgt tagccgtgat atgatgatcg agatccttgc
      841 cgatgaagaa ggtcatattg actggctcga aactgagctg gatctcatcg gtaaaatcgg
      901 tttacaaaat tacctgcaat cacaaataaa agttaaagat taacggcctg ctagcaaact
      961 atcccaacct acaacgaaac ccgcagccgc cagaaatggc ccaaacggca gcgggttttt
     1021 taatgactct tttcctctca tgagcagacc aacaactacg gctccgcaag cgaatgaggc
     1081 agcaaggaaa accagtcgtg gcaagaaggt ccaggtatgc caggcaccaa gggcggcgag
     1141 aaacttcaca tcaccatagc ctaatccttc tttatgacgc agtatgcgat acccccagta
     1201 aatgacagcg aatgtgccgt aaccaataat cgccccccat aacgcatcgg ctaaaccgtc
     1261 aggattacaa acctgagaga acaaaagacc tgaccagagt aacgggcagg taaatctgtc
     1321 aggtaacaag ccatgctttg catcccataa aaaaagtagg acagataaac aagcgtacaa
     1381 aatcagaaag ggaagggtag cgtccataaa acatcctctg taattggagt atcaccatcg
     1441 aataactccg gatgctcagc aacagagaaa tcgctgattt gttaacctgg ttccggtgaa
     1501 aaacgctatc ttcacaacct tacaaatttc gtggaataca gctcgcaatc tgcgcgttgc
     1561 gattaatttc tacccgatgc ttgcgtcagg ctcagatttg cgtataatgc gcgggcttgt
     1621 cgtagttgac agcaggttca atctgaacct cagttagccg aatttggcta cctaacaatg
     1681 ctcccaatcg gggagctacg taagaacggt tacactctcc catcaatcgt aatgggtctg
     1741 aggagtaatc attttcgttt ataaaataat tggagctctg gtctcatgca gaaccaaaga
     1801 atccgtatcc gcctgaaagc gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc
     1861 gtcgagactg ccaagcgcac tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc
     1921 aaagagcgct tcactgttct gatctccccg cacgtcaaca aagacgcgcg cgatcagtac
     1981 gaaatccgta ctcacttgcg tctggttgac atcgttgagc caaccgagaa aaccgttgat
     2041 gctctgatgc gtctggatct ggctgccggt gtagacgtgc agatcagcct gggttaatca
     2101 ggtcattgag cgattgagag gttgaaacaa tgattggttt agtcggtaaa aaagtgggta
     2161 tgacccgtat cttcacagaa gacggcgttt ctatcccagt aaccgtaatc gaagttgaag
     2221 caaaccgcgt tactcaggtt aaagacctgg ctaacgatgg ctaccgtgct attcaggtga
     2281 ccaccggtgc taaaaaagct aaccgtgtga ccaagcctga agctggccac ttcgctaaag
     2341 ctggcgtaga agctggccgt ggtctgtggg aattccgcct ggctgaaggc gaagagttca
     2401 ctgtaggtca gagcattagc gttgaactgt ttgctgacgt taaaaaagtt gacgtaactg
     2461 gcacctctaa aggtaaaggt ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg
     2521 acgctactca cggtaactcc ttgtctcacc gcgttccggg ttctatcggt cagaaccaga
     2581 ctccgggcaa agtgttcaaa ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa
     2641 ccgttcagag ccttgacgta gtacgcgttg acgctgagcg caacctgctg ctggttaaag
     2701 gtgctgtccc gggtgcaacc ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag
     2761 gagatagcaa tggaattagt attgaaagac gcgcagagcg cgctgactgt ttccgaaact
     2821 accttcggtc gtgatttcaa cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct
     2881 ggtgctcgtc agggtactcg tgctcagaag actcgtgctg aagtaactgg ttccggtaaa
     2941 aaaccgtggc gccagaaagg caccggccgt gcgcgttctg gttctatcaa gagcccgatc
     3001 tggcgttctg gtggcgtgac ctttgctgct cgtccgcagg accacagtca aaaagttaac
     3061 aagaagatgt accgcggcgc gctgaaaagc atcctgtccg aactggtacg tcaggatcgt
     3121 ctgatcgttg tcgagaagtt ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa
     3181 ctgaaagaca tggctctgga agatgtgctg atcatcaccg gtgagctgga cgaaaacctg
     3241 ttcctggctg cgcgcaacct gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg
     3301 gttagcctga tcgccttcga caaagtcgta atgactgctg atgctgttaa gcaagttgag
     3361 gagatgctgg catgattcgt gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt
     3421 ctgaaaaagc gtctactgcg atggaaaaat ccaacaccat cgtactcaaa gttgctaaag
     3481 acgcgaccaa agcagaaatc aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag
     3541 tcgttaacac cctggtagtt aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc
     3601 gtagcgactg gaaaaaagct tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg
     3661 gcggcgctga gtaagtcgga ggagtaatac aatggcagtt gttaaatgta aaccgacatc
     3721 tccgggtcgt cgccacgtag ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt
     3781 tgctccgttg ctggaaaaaa acagcaaatc cggtggtcgt aacaacaatg gccgtatcac
     3841 cactcgtcat atcggtggtg gccacaagca ggcttaccgt attgttgact tcaaacgcaa
     3901 caaagacggt atcccggcag ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa
     3961 catcgcgctg gttctgtaca aagacggtga acgccgttac atcctggccc ctaaaggcct
     4021 gaaagctggc gaccagattc agtctggcgt tgatgctgca atcaaaccag gtaacaccct
     4081 gccgatgcgc aacatcccgg ttggttctac tgttcataac gtagaaatga aaccaggtaa
     4141 aggcggtcag ctggcacgtt ccgctggtac ttacgttcag atcgttgctc gtgatggtgc
     4201 ttatgtcacc ctgcgtctgc gttctggtga aatgcgtaaa gtagaagcag actgccgtgc
     4261 aactctgggc gaagttggca atgctgagca tatgctgcgc gttctgggta aagcaggtgc
     4321 tgcacgctgg cgtggtgttc gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca
     4381 cccacatggt ggtggtgaag gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt
     4441 tcagaccaaa ggtaagaaga cccgcagcaa caagcgtact gataaattca tcgtacgtcg
     4501 ccgtagcaaa taattttaga ggataagcca tgccacgttc tctcaagaaa ggtcctttta
     4561 ttgacctgca cttgctgaag aaggtagaga aagcggtgga aagcggagac aagaagcccc
     4621 tgcgcacttg gtcccgtcgt tcaacgatct ttcctaacat gatcggtttg accatcgctg
     4681 tccataatgg tcgtcagcac gttccggtat ttgtaaccga cgaaatggtc ggtcacaaac
     4741 tgggtgaatt cgcaccgact cgtacttatc gcggccacgc tgctgataaa aaagcgaaga
     4801 agaaataagg taggaggaag agatggaaac tatcgctaaa catcgccatg ctcgttcttc
     4861 tgctcagaag gttcgccttg ttgctgacct gattcgcggt aagaaagtgt cgcaggctct
     4921 ggatattttg acctacacca acaagaaagc ggctgtactg gtcaagaaag ttctggaatc
     4981 tgccattgct aacgctgaac acaacgatgg cgctgacatt gacgatctga aagttacgaa
     5041 aattttcgta gacgaaggcc cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc
     5101 agatcgcatc ctgaagcgca ccagccacat cactgtggtt gtgtccgatc gctgagactc
     5161 tggagactag caatgggtca gaaagtacat cctaatggta ttcgcctggg tattgtaaaa
     5221 ccatggaact ctacctggtt tgcgaacacc aaagaattcg ctgacaacct ggacagcgat
     5281 tttaaagtac gtcagtacct gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt
     5341 atcgagcgtc cggctaagag catccgtgta accattcaca ctgctcgccc gggtatcgtt
     5401 atcggtaaaa aaggtgaaga cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc
     5461 gttcctgcac agatcaacat cgccgaagtt cgtaagcctg aactggacgc aaaactggtt
     5521 gctgacagca tcacttctca gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt
     5581 gctgtacaga acgcaatgcg tctgggcgct aaaggtatta aagttgaagt tagcggccgt
     5641 ctgggcggcg cggaaatcgc acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac
     5701 actctgcgtg ctgacatcga ctacaacacc tctgaagcgc acaccactta cggtgtaatc
     5761 ggcgttaaag tgtggatctt caaaggcgag atcctgggtg gtatggctgc tgttgaacaa
     5821 ccggaaaaac cggctgctca gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg
     5881 tcgctgatgt tacaaccaaa gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc
     5941 ggtctggcgc agggtacgga tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt
     6001 ggtcgtctga ctgcccgtca gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag
     6061 cgtcaaggta agatctggat ccgtgtgttc ccggacaaac cgatcactga aaagccgctg
     6121 gcagtgcgta tgggtaaagg taaaggtaac gtggagtatt gggttgcctt gattcagccg
     6181 ggtaaagtcc tgtatgaaat ggacggtgtt ccggaagagc tggcccgtga agcattcaag
     6241 ctggcagcag cgaaactgcc gattaaaacc acctttgtaa ctaagacggt gatgtaatga
     6301 aagcaaaaga gctgcgtgag aagagcgttg aagagctgaa caccgagctg ctgaacctgc
     6361 tgcgtgagca gttcaacctg cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc
     6421 tgttgaagca agtgcgtcgc gatgtcgcac gcgttaagac tttactgaac gagaaggcgg
     6481 gtgcgtaatg accgataaaa tccgtactct gcaaggtcgc gttgttagcg acaaaatgga
     6541 gaaatccatt gttgttgcta tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat
     6601 caagcgtacg accaaactgc acgtacatga cgagaacaac gaatgcggta tcggtgacgt
     6661 ggttgaaatc cgcgaatgcc gtccgctgtc caagactaaa tcctggacgc tggttcgcgt
     6721 tgtagagaaa gcggttctgt aatacagtac actctctcga tacgaataaa cggctcagaa
     6781 atgagccgtt tattttttct acccatatcc ttgaagcggt gttataatgc cgcgccctcg
     6841 atatggggat ttttaacgac ctgattttcg ggtctcagta gtagttgaca ttagcggagc
     6901 actaaaatga tccaagaaca gactatgctg aacgtcgccg acaactccgg tgcacgtcgc
     6961 gtaatgtgta tcaaggttct gggtggctcg caccgtcgct acgcaggcgt aggcgacatc
     7021 atcaagatca ccatcaaaga agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg
     7081 aaggcggtag tggtgcgcac caagaagggt gttcgtcgcc cggacggttc tgtcattcgc
     7141 ttcgatggta atgcttgtgt tcttctgaac aacaacagcg agcagcctat cggtacgcgt
     7201 atttttgggc cggtaactcg tgagcttcgt agtgagaagt tcatgaaaat tatctctctg
     7261 gcaccagaag tactctaagg agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt
     7321 tatcgtgtta accggtaaag ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc
     7381 cggcaaggtc attgttgaag gtatcaacct ggttaagaaa catcagaagc cggttccggc
     7441 cctgaaccaa ccgggtggca tcgttgaaaa agaagccgct attcaggttt ccaacgtagc
     7501 aatcttcaat gcggcaaccg gcaaggctga ccgtgtaggc tttagattcg aagacggcaa
     7561 aaaagtccgt ttcttcaagt ctaacagcga aactatcaag taatttggag tagtacgatg
     7621 gcgaaactgc atgattacta caaagacgaa gtagttaaaa aactcatgac tgagtttaac
     7681 tacaattctg tcatgcaagt ccctcgggtc gagaagatca ccctgaacat gggtgttggt
     7741 gaagcgatcg ctgacaaaaa actgctggat aacgcagcag cagacctggc agcaatctcc
     7801 ggtcaaaaac cgctgatcac caaagcacgc aaatctgttg caggcttcaa aatccgtcag
     7861 ggctatccga tcggctgtaa agtaactctg cgtggcgaac gcatgtggga gttctttgag
     7921 cgcctgatca ctattgctgt acctcgtatc cgtgacttcc gtggcctgtc cgctaagtct
     7981 ttcgacggtc gtggtaacta cagcatgggt gtccgtgagc agatcatctt cccagaaatc
     8041 gactacgata aagtcgaccg cgttcgtggt ttggatatta ccattaccac tactgcgaaa
     8101 tctgacgaag aaggccgcgc tctgctggct gcctttgact tcccgttccg caagtaaggt
     8161 agggttacta aatggctaag caatcaatga aagcacgcga agtaaaacgc gtagctttag
     8221 ctgataaata cttcgcgaaa cgcgctgaac tgaaagcgat catctctgat gtgaacgctt
     8281 ccgacgaaga tcgttggaac gctgttctca agctgcagac tctgccgcgt gattccagcc
     8341 cgtctcgtca gcgtaaccgc tgccgtcaaa caggtcgtcc gcatggtttc ctgcggaagt
     8401 tcgggttgag ccgtattaag gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga
     8461 aaaaggctag ctggtaattg tcaccaattg aatcacggga gtaaagacag atgagcatgc
     8521 aagatccgat cgcggatatg ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg
     8581 cggtcaccat gccttcctcc aagctgaaag tggcaatcgc caacgtgctg aaggaagaag
     8641 gttttattga agattttaaa gttgaaggcg acaccaagcc tgaactggaa cttactctga
     8701 agtatttcca gggcaaagct gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc
     8761 gcatctataa acgtaaagat gagctgccga aagttatggc gggtctgggt atcgcagttg
     8821 tttctacctc taaaggtgtt atgactgatc gtgcagcgcg ccaggctggt cttggtggcg
     8881 aaattatctg ctacgtagcc taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg
     8941 tcgttgttcc tgccggcgtt gacgtaaaaa tcaacggtca ggttattacg atcaaaggta
     9001 aaaacggcga gctgactcgt actctcaacg atgctgttga agttaaacat gcagataata
     9061 ccctgacctt cggtccgcgt gatggttacg cagacggttg ggcacaggct ggtaccgcgc
     9121 gtgccctgct gaactcaatg gttatcggtg ttaccgaagg cttcactaag aagctgcagc
     9181 tggttggtgt aggttaccgt gcagcggtta aaggtaatgt gattaacctg tctctgggtt
     9241 tctctcatcc tgttgaccat cagctgcctg cgggtatcac tgctgaatgt ccgactcaga
     9301 ctgaaatcgt gctgaaaggc gctgataagc aggtgatcgg ccaggttgca gcggatctgc
     9361 gcgcctaccg tcgtcctgag ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg
     9421 tgcgtaccaa agaggctaag aagaagtaag gtaacactat ggataagaaa tctgctcgta
     9481 tccgtcgtgc gacccgcgca cgccgcaagc tccaggagct gggcgcaact cgcctggtgg
     9541 tacatcgtac cccgcgtcac atttacgcac aggtaattgc accgaacggt tctgaagttc
     9601 tggtagctgc ttctactgta gaaaaagcta tcgctgaaca actgaagtac accggtaaca
     9661 aagacgcggc tgcagctgtg ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca
     9721 aagatgtatc ctttgatcgt tccgggttcc aatatcatgg tcgtgtccag gcactggcag
     9781 atgctgcccg tgaagctggc cttcagttct aaggtagagg tgtaagatgg ctcacatcga
     9841 aaaacaagct ggcgaactgc aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt
     9901 taaaggtggt cgtattttct ccttcacagc tctgactgta gttggcgatg gtaacggtcg
     9961 cgttggtttt ggttacggta aagcgcgtga agttccagca gcgatccaga aagcgatgga
    10021 aaaagcccgt cgcaatatga ttaacgtcgc gctgaataac ggcactctgc aacaccctgt
    10081 taaaggtgtt cacacgggtt ctcgcgtatt catgcagccg gcttccgaag gtaccggtat
    10141 catcgccggt ggtgcaatgc gcgccgttct ggaagtcgct ggggttcata acgttctggc
    10201 taaagcatat ggttccacca acccgatcaa cgtggttcgt gcaactattg atgggctgga
    10261 aaatatgaat tctccagaaa tggtcgctgc caagcgtggt aaatccgttg aagaaattct
    10321 ggggaaataa accatggcaa agactattaa aattactcaa acccgcagtg caatcggtcg
    10381 tctgccgaaa cacaaggcaa cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt
    10441 agagcgcgag gatactcctg ctattcgcgg tatgatcaac gcggtttcct tcatggttaa
    10501 agttgaggag taagagatgc gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc
    10561 gggtaaacgc ctgggtcgtg gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca
    10621 caaaggtcag aagtctcgtt ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat
    10681 gcctctgtac cgtcgtctgc cgaaattcgg cttcacttct cgtaaagcag cgattacagc
    10741 cgaagttcgt ctgtctgacc tggctaaagt agaaggcggt gttgtagacc tgaacacgct
    10801 gaaagcggct aacattatcg gtatccagat cgagttcgcg aaagtgatcc tggctggcga
    10861 agtaacgact ccggtaactg ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat
    10921 cgaagctgct ggcggtaaaa tcgaggaata agtagcagat ggctaaacaa ccgggattag
    10981 attttcaaag tgccaaaggt ggcttaggcg agctgaaacg cagactgctg tttgttatcg
    11041 gtgcgctgat tgtgttccgt attggctctt ttattccgat ccctggtatt gatgccgctg
    11101 tacttgccaa actgcttgag caacagcgag gcaccatcat tgagatgttt aacatgttct
    11161 ctggtggtgc tctcagccgt gcttctatct ttgctctggg gatcatgccg tatatttcgg
    11221 cgtcgatcat tatccagctg ctgacggtgg ttcacccaac gttggcagaa attaagaaag
    11281 aaggggagtc tggtcgtcgt aagatcagcc agtacacccg ttacggtact ctggtgctgg
    11341 caatattcca gtcgatcggt attgctaccg gtctgccgaa tatgcctggt atgcaaggcc
    11401 tggtgattaa cccaggcttt gcattctact tcaccgctgt tgtaagtctg gtcacaggaa
    11461 cgatgttcct gatgtggttg ggcgaacaga ttactgaacg aggtatcggc aacggtattt
    11521 caatcattat cttcgccggt attgtcgcgg gactcccgcc agccattgcc catactatcg
    11581 agcaagcgcg tcaaggcgac ctgcacttcc tcgtgttgct gttggttgca gtattagtat
    11641 ttgcagtgac gttctttgtt gtatttgttg agcgtggtca acgccgcatt gtggtaaact
    11701 acgcgaaacg tcagcaaggt cgtcgtgtct atgctgcaca gagcacacat ttaccgctga
    11761 aagtgaatat ggcgggggta atcccggcaa tcttcgcttc cagtattatt ctgttcccgg
    11821 cgaccatcgc gtcatggttc gggggcggta ctggttggaa ctggctgaca acaatttcgc
    11881 tgtatttgca gcctgggcaa ccgctttatg tgttactcta tgcgtctgca atcatcttct
    11941 tctgtttctt ctacacggcg ttggttttca acccgcgtga aacagcagat aacctgaaga
    12001 agtccggtgc atttgtacca ggaattcgtc cgggagagca aacggcgaag tatatcgata
    12061 aagtaatgac ccgcctgacc ctggttggtg cgctgtatat tacctttatc tgcctgatcc
    12121 cggagttcat gcgtgatgca atgaaagtac cgttctactt cggtgggacc tcactgctta
    12181 tcgttgttgt cgtgattatg gactttatgg ctcaagtgca aactctgatg atgtccagtc
    12241 agtatgagtc tgcattgaag aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc
    12301 gagaagttac ggagagtaaa aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac
    12361 tgcaaaatcg ttaagcgtga tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat
    12421 aaacagcgcc aaggctgatt ttttcgcata tttttcttgc aaagttgggt tgagctggct
    12481 agattagcca gccaatcttt tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg
    12541 gcttttcagc atggaacgta catattaaat agtaggagtg catagtggcc cgtatagcag
    12601 gcattaacat tcctgatcat aagcatgccg taatcgcatt aacttcgatt tatggcgtcg
    12661 gcaagacccg ttctaaagcc atcctggctg cagcgggtat cgctgaagat gttaagatca
    12721 gtgagctgtc tgaaggacaa atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg
    12781 aaggtgatct gcgccgtgaa atcagcatga gcatcaagcg cctgatggat cttggttgct
    12841 atcgcggttt gcgtcatcgt cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg
    12901 cacgtacccg taagggtccg cgcaaaccga tcaagaaata atcggggtga ttgaataatg
    12961 gcaaaggcac caattcgtgc acgtaaacgt gtaagaaaac aagtctctga cggcgtggct
    13021 catatccatg cttctttcaa caacaccatc gtgactatca ctgatcgtca gggtaacgcg
    13081 ttgggttggg caacagccgg tggttccggt ttccgtggtt ctcgcaaatc cactccgttt
    13141 gcagctcagg ttgcagcaga gcgttgcgct gacgccgtga aagaatacgg catcaagaat
    13201 ctggaagtta tggttaaagg tccgggtcca ggccgcgaat ctactattcg tgctctgaac
    13261 gccgcaggtt tccgcatcac taacattact gatgtgactc cgatccctca taacggttgt
    13321 cgtccgccga aaaaacgtcg cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa
    13381 atggcaagat atttgggtcc taagctcaag ctgagccgtc gtgagggcac cgacttattc
    13441 cttaagtctg gcgttcgcgc gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag
    13501 cacggtgcgc gtaaaccgcg tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa
    13561 gttcgccgta tctatggtgt gctggagcgt cagttccgta actactacaa agaagcagca
    13621 cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc tggaaggtcg tctggacaac
    13681 gttgtatacc gtatgggctt cggtgccact cgtgcagaag cacgtcagct ggttagccat
    13741 aaagcaatta tggtaaacgg tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat
    13801 gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag
    13861 ctggctgagc agcgtgaaaa gccaacctgg ctggaagttg atgctggcaa gatggaaggt
    13921 acgtttaagc gtaagccgga gcgttctgat ctgtctgcgg acattaacga acacctgatc
    13981 gtcgagcttt actccaagta aagcttagta ccaaagagag gacacaatgc agggttctgt
    14041 gacagagttt ctaaaaccgc gcctggttga tatcgagcaa gtgagttcga cgcacgccaa
    14101 ggtgaccctt gagcctttag agcgtggctt tggccatact ctgggtaacg cactgcgccg
    14161 tattctgctc tcatcgatgc cgggttgcgc ggtgaccgag gttgagattg atggtgtact
    14221 acatgagtac agcaccaaag aaggcgttca ggaagatatc ctggaaatcc tgctcaacct
    14281 gaaagggctg gcggtgagag ttcagggcaa agatgaagtt attcttacct tgaataaatc
    14341 tggcattggc cctgtgactg cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa
    14401 gccgcagcac gtgatctgcc acctgaccga tgagaacgcg tctattagca tgcgtatcaa
    14461 agttcagcgc ggtcgtggtt atgtgccggc ttctacccga attcattcgg aagaagatga
    14521 gcgcccaatc ggccgtctgc tggtcgacgc atgctacagc cctgtggagc gtattgccta
    14581 caatgttgaa gcagcgcgtg tagaacagcg taccgacctg gacaagctgg tcatcgaaat
    14641 ggaaaccaac ggcacaatcg atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc
    14701 tgaacaactg gaagctttcg ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga
    14761 gaaaccagag ttcgatccga tcctgctgcg ccctgttgac gatctggaat tgactgtccg
    14821 ctctgctaac tgccttaaag cagaagctat ccactatatc ggtgatctgg tacaacgtac
    14881 cgaggttgag ctccttaaaa cgcctaacct tggtaaaaaa tctcttactg agattaaaga
    14941 cgtgctggct tcccgtggac tgtctctggg catgcgcctg gaaaactggc caccggcaag
    15001 catcgctgac gagtaaccgg atcacaggtt aaggttttac tgagaaggat aaggtcatgc
    15061 gccatcgtaa gagtggtcgt caactgaacc gcaacagcag ccatcgccag gctatgttcc
    15121 gcaatatggc aggttcactg gttcgtcatg aaatcatcaa gacgactctg cctaaagcga
    15181 aagagctgcg ccgcgtagtt gagccgctga ttactcttgc caagactgat agcgttgcta
    15241 atcgtcgtct ggcattcgcc cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg
    15301 aactgggccc gcgtttcgcg agccgtgccg gtggttacac tcgtattctg aagtgtggct
    15361 tccgtgcagg cgacaacgcg ccgatggctt acatcgagct ggttgatcgt tcagagaaag
    15421 cagaagctgc tgcagagtaa tctgaagcaa cgtaaaaaaa cccgccccgg cgggtttttt
    15481 tatacccgta gcatccccac ttatctacaa tagctgtact ctttttgttc atcccctgga
    15541 gtatttatgt ggttacttga ccagtgggca gagcgtcata tagcagaagc gcaagcgaaa
    15601 ggtgagtttg ataacctggc aggtagcggc gaaccattga tactggatga tgattctcac
    15661 gtgctaccgg aattacgtgc ggggtatcgc ttgctgaaga atgccggttg cttaccgcca
    15721 gaacttgagc aacggagaga agcaattcag cttctggata tcctgagagg tatccgtcac
    15781 gatgatccgc aatatcaaga ggttagccgt cgattgtcat tactggaatt gaagctgcga
    15841 caagctggat tgagtaccga ttttttacgc ggcgattatg ctgacaagtt gttggacaaa
    15901 atcaacgata actagtggag tatgtatgta tcgcattggt gagctggcaa aaatggcgga
    15961 agtaacacct gacacgattc gttattacga aaaacagcag atgatggagc atgaagtgcg
    16021 tactgaaggc gggtttcgcc tgtataccga aagcgatctc cagcgattga aatttatccg
    16081 ccatgccaga caactaggtt tcagtctgga gtcgatccgc gagttgctgt cgatccgcat
    16141 cgatcctgaa caccatacct gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga
    16201 agtcgaagca cggatagccg agttgcagag tatgcagcgt tctttgcaac gccttaacga
    16261 tgcctgttgt gggaccgccc atagcagtgt ttattgttcg attcttgaag ctcttgaaca
    16321 aggggcgagt ggcgttaaga gtggctgttg attttttgca ctggcaggat tacactcgcg
    16381 ccgtaaaata accaattgga gtttttatga gccgatatca gcatactaaa gggcagataa
    16441 aggataatgc gatagaagca ttactacatg atcccttatt ccgacagcgc gtagagaaaa
    16501 ataagaaggg gaaaggcagt tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg
    16561 aggccagtgg caagaaagta aatcactttt ttaccactgg tcttctgctt tcaggcgctt
    16621 gttaagagcg gttattctgc tctttcagca aatcacgaat ttctgccagt aatacttctt
    16681 ctttagttgg tgcaggtgcg gcagctggtt cttctttttt ccgattcagt ttgttgatta
    16741 gcttaatcgc cataaagatg gcaaaggcca caatcagaaa atcaaagacg ttttgaatga
    16801 agacaccgta atgcatcaca acagcaggga tatccccctg cgcatcgcgt agcgtgacag
    16861 caaactgttt aaagtcgatc ccgccaatta ataagcccag aggtggcatg atgatatcgg
    16921 caaccagtga agatacaatc ttcccgaatg ccgcaccgat aatgacaccc accgccaaat
    16981 ccaccacgtt cccgcgcatc gcaaattcgc gaaattcttt aataatgctc atgttattct
    17041 ccctatgaag ccgacaacca taagtctaac aaatgttaag ccattttcct gccaggaatg
    17101 aatttaatta atccttgata atcaaaggga aataataagg cgtcattaga cgccttatta
    17161 attacaagaa gaaagggctt ggctggaaga gtctttcgac gtcggtaata aactttttat
    17221 ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg cagattgtca ttggcaatca
    17281 tcacgtcgtt tccacgtacc accgctccaa taatcgttcc tggcggtagc ttgatttcgt
    17341 caatgactct gccgacaacg cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag
    17401 cttctgctac gccgcggcgc aatgaggaaa caccaacaat atctgctttt cgcacatggc
    17461 taagcaacgc agaaatagtt gcttgttgtg gtgaaattgc aatatcgatc acgctcccct
    17521 gaaccagatc cacataagcg cgacgctgga tcaataccat cacctttttc gcacccatac
    17581 gtttggcaag catggcggac atgatattgg cctcgtcatc gttggtgacg gcaataaaca
    17641 gatcaacttg atcgatatgt tcttcggcca gcagttcttg atccgacgca tcaccaaaaa
    17701 agacgatcgt attctgcaac ttttccgcca gttcggcagc gcgctgctga ttacgttcga
    17761 tgagtttaac gctgtaatct ttttccagac gacgcgccag ccctgcaccg atattaccgc
    17821 caccaaccag catgatccgc ttatacggtt tttccagtcg ctgtaattca ctcatcaccg
    17881 cacggatatg ctgtgaagcg gcaataaaga acacttcatc accagcttcc acaatggtcg
    17941 aaccttgtgg acgaatgggg cgatcgtggc ggaaaattgc tgccacacga gtatcgatat
    18001 gtggcatatg ttcgcgcatg gtcgaaagtg cgttaccaat cagcgggccg ccataatagg
    18061 ctttaaccac agcgaggctg actttaccct cagcgaagtt caccacctgc aatgcgccgg
    18121 gatactcaat cagtcggtaa atattatcga taaccaactg ctctggtgcg atcagatgat
    18181 caatcggtac agcatctgaa tgaaatagct tatcggcatc gcgcacgtag tctggtgagc
    18241 ggatgcgagc gatgcgatta ggggtgttga aaagtgagta ggctacctgg caggcaacca
    18301 tattggtttc atctgaactg gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct
    18361 cccgcaatac gcgtggatga gagccatgcc cctgcacgac tcgcaggtca aatttatcct
    18421 gcaaggtccg cagacgctca ccgttggtat cgacaacagt gatatcgttg ttctcgccaa
    18481 ccaggttttc cgccagtgtg ccgccaacct ggccggcacc cagaatgata attttcatca
    18541 gtcgcgaccc gttatctcat cactttttga ttagcttagc gtaaaagaag ccgtcgccct
    18601 cttcggcacc aggcagattt tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt
    18661 cggcatcagc ggtacgttgc aaaaaggctt taatctgcag gctattctct tccggtaaca
    18721 ccgaacaggt ggcatagacc agggttccac cggattttaa atgcggccaa atggcgtcga
    18781 gaatttcaga ctgcaattgc gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa
    18841 tatccggatg gcgacgaatc acaccggttg ctgaacaagg cgcatctaat aaaatgcgat
    18901 caaactgttg ctcgccacac cattgggaag ggtagcggcc atcaccttgt ttcacggtcg
    18961 ctttcatacc aaggcgtttt aaattgtcgt aaacgcgaga gaggcgctgt tcgtcaatat
    19021 caaccgcaac aacctgcgct tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg
    19081 gggcggcaca aagatccaga atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac
    19141 cttgtgctga tgcatcctga acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa
    19201 caggtgcagg tgtttccagc cgtacagcat cagggtaatc cgcatgcggg aaacctttca
    19261 ttcctgcttc atccaacaat gcaagccagc tgtcgcggga atgatgcgta cgattaacgc
    19321 gcagccacat tggcggacgc tggttattgg cttcgacgat ggattgccac tgctctggat
    19381 acgctttttg cagacgcttc agcaaccagg aaggatgcag ataacgtgca tcactggcat
    19441 taaactcggc taataactct tcttgctgac gctggaactg gcgtaataca ccgtttatca
    19501 accctttaag ttgcggacgc ttaattgcga tagcgccttc aaccgtttca gccagcgcag
    19561 catgaggtgg aatgcgggta taaagcagtt gatacaaacc aaccataatc aggtaatgca
    19621 cggtccgctg tttgccggtc atcggacggg ccattaactt attaatcagc cagtctaact
    19681 gcgaaagcgt acgcagtacg ccaaagcaca actcttgaag aagtgctttg tctttatcgg
    19741 aaactttttg ctggagcggt ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt
    19801 gttcaacggc ctgggccgcc atgctacgta aattacgttg ttttttcata agtataaaaa
    19861 cgcccggcaa gaccgggctt agaagagtgg actatcagac cagacggttg cccggaacaa
    19921 accattcccg acgagagttc aggaggtctt gcgcgctcat cgctttctta cccgcaggtt
    19981 gtaacgagag caggttcagg atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg
    20041 cttcaaggat cgttcctggt gcagcgttgg ttgccgtatc aatgaccgat gctttccaga
    20101 ctttaaccgg ctgtccttca atttccagcc agctcattgg ccatggattg aaagcgcgaa
    20161 tgcagcgttc aagctgtgct gccgaaagtg accagtcaat acgcgcttct tctttactca
    20221 acttttcggc gtaagtgaca agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg
    20281 ccagttgttt caacgtggtg ataagccctt gtgggccaag ctctgccagc ttgtcgtaca
    20341 gcgtaccact ggtatcttct gcagtaatcg ggcaggagag cttatagagc atgtcaccgg
    20401 tgtctaaacc gacatccatt tgcataatgg tcacaccagt ttctgcatca cccgcccata
    20461 gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga
    20521 tacagccaag acgcggcatc tccagcactg ctttaggcag aattaagcca taggcgacga
    20581 cgaccataac atcagcctgc agatcggcga ccagttgctg gttttcttgt ggacgcaggg
    20641 aaacaggttg aaaaacgggc agaccttttt cctcagccag aactttaacc gggctgggca
    20701 tcagtttttt accgcgtcct gccggtcggt ctggctgggt gaacacgcca acgacgttat
    20761 gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa
    20821 taatacgtag tgattctgac acgttagttc ttatccttaa gcccgggctt tcagacgatc
    20881 cagtttttca actttctgac gaatacgttg ttgtttcaac ggtgacagat aatccataaa
    20941 cagtttgccg accaggtgat ccatctcatg ctgaatacag atggctaaca gaccgtctgc
    21001 ttccagttcg aatggtttac cgtcgcggtc cagggcgcga attttaactt tctctgcgcg
    21061 tggcactaaa gcacgttgtt cagggatcga caggcaacct tcttcaatgc ctgtttcgcc
    21121 gcttttttct aaaagctccg gattgattaa caccagccgt tcgtcacggt tttccgaaac
    21181 atcaataaca atgatacgtt gatggatatc aacctgggtt gccgccaggc caataccttc
    21241 ttctgcgtac atcgtctcga acatatcatc gacgatacgc tgaatttctg cattcacttc
    21301 ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac
    21361 tgacataaat ctccagagat gtgttcagga gttagaaaga ttatttcttc tattctagac
    21421 aaatccccct ctgattgaca gcatcactga ccaatcgcaa agattgctaa ggctgcttat
    21481 ggcagggaga taaggatggt cgatacagat atttggctgc gtttaatgag catcagcagc
    21541 ttgtacggcg atgatatggt ccgtatagct cactggctgg caaaacagtc gcatattgat
    21601 gcggttggat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt tctttcattt
    21661 ccgcgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca tcatttaatc
    21721 cctgcggaca gcgaatttta tcctcctcaa cttctggcga cgacagatta ccccggcgca
    21781 ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt agtggggagt
    21841 cgggcgcatt catggtatgg cgagcgatgg ggacgattat tttgcgaaac tctggccacg
    21901 cgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggc gcataaagcg
    21961 gccttacagg taaatggcgt cagcattgct gtattgggga acggacttaa taccattcat
    22021 ccgcgccgcc atgcccgact ggctaccagt ttgcttgaac atggtggggc acttgtctcg
    22081 gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc
    22141 agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgttggtg
    22201 acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg accaataggg
    22261 aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa
    22321 ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa
    22381 aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga gctcctggct
    22441 aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg
    22501 ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc
    22561 ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga
    22621 aacctatatt cacacagaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct
    22681 taccgacgca ggatttgatc gagaagatat ctacaatgcc ctgctttggc tggaaaagct
    22741 tgcggattat caggaaggac tggcagaacc gatgcaactg gcctctgatc cactctccat
    22801 gcgtatttat acccctgagg agtgtgaaag actggatgcc agctgtcgtg gttttctgct
    22861 tttccttgag caaattcagg tgcttaacct tgaaacccgt gaaatggtga tagagcgagt
    22921 gcttgcgctg gataccgcag agttcgacct ggaagacctg aaatgggtaa tcctgatggt
    22981 gttgttcaat attccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga
    23041 agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcga
    23101 aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg
    23161 ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact
    23221 acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg
    23281 tttgccctgc atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg
    23341 gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag
    23401 caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc cgttatggca
    23461 aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccca
    23521 tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa accgcgcagg
    23581 gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac
    23641 gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct caatgaaaaa
    23701 cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc tgatagcgaa
    23761 acagcagtga tgcgactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta
    23821 atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgct gactgatgcg
    23881 cagcgtgaaa ccattttttc ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct
    23941 gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac
    24001 catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc
    24061 aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg
    24121 gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat
    24181 gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg
    24241 taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa
    24301 tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa
    24361 cgctttcttt agtgctggtg gtaaaggtgc gaatgtgacg gtacctttta aagaagaggc
    24421 ttttgccaga gcggatgagc ttactgaacg ggcagcgtta gctggtgctg ttaataccct
    24481 caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag gcttgttaag
    24541 cgatctggaa cgtctgtctt ttatccgtcc tggtttacgt attctgctca tcggcgctgg
    24601 tggagcttct cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac
    24661 taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat
    24721 tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac
    24781 atccagtggc atcagtggtg atattccggc gatcccgtca tcgcttattc atccaggcat
    24841 ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca
    24901 gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc
    24961 ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc aattgcagga
    25021 ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg ggacgagaat
    25081 aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg cgcgatctct
    25141 ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt agcgagtttt
    25201 cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga acaaagtgaa
    25261 gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa
    25321 ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg
    25381 acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg gaccagacta
    25441 cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc
    25501 ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg
    25561 acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca
    25621 tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct
    25681 ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc
    25741 acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag atcgcggtat
    25801 gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat gactttgggg
    25861 gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgatt
    25921 gcagcaaaaa gggtgaaaaa acaacaaaca gaaaaaaaga tcaaaaaaat acttgtgcaa
    25981 aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacaaga
    26041 tggtcgtaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga aagaggaaag
    26101 cgtaatatac gccacctcgc gacagtgagc tgaaagc
//
LOCUS       DAJSKJ010000039        25822 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000039 DAJSKJ010000000
VERSION     DAJSKJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.039"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..121)
                     /locus_tag="ON345_001411"
     CDS             complement(<1..121)
                     /locus_tag="ON345_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR3071497.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGK"
     gene            313..1518
                     /gene="csdA"
                     /locus_tag="ON345_001412"
     CDS             313..1518
                     /gene="csdA"
                     /locus_tag="ON345_001412"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR3071498.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            1518..1961
                     /gene="csdE"
                     /locus_tag="ON345_001413"
     CDS             1518..1961
                     /gene="csdE"
                     /locus_tag="ON345_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR3071499.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2012..2818)
                     /gene="tcdA"
                     /locus_tag="ON345_001414"
     CDS             complement(2012..2818)
                     /gene="tcdA"
                     /locus_tag="ON345_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR3071500.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2895..3992)
                     /gene="mltA"
                     /locus_tag="ON345_001415"
     CDS             complement(2895..3992)
                     /gene="mltA"
                     /locus_tag="ON345_001415"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR3071501.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            4201..4277
                     /locus_tag="ON345_001416"
     tRNA            4201..4277
                     /locus_tag="ON345_001416"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4235..4237,aa:Met,seq:cat)
     gene            complement(4352..5605)
                     /gene="amiC"
                     /locus_tag="ON345_001417"
     CDS             complement(4352..5605)
                     /gene="amiC"
                     /locus_tag="ON345_001417"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR3071502.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            5837..7168
                     /gene="argA"
                     /locus_tag="ON345_001418"
     CDS             5837..7168
                     /gene="argA"
                     /locus_tag="ON345_001418"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR3071503.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLNKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7230..9056)
                     /gene="recD"
                     /locus_tag="ON345_001419"
     CDS             complement(7230..9056)
                     /gene="recD"
                     /locus_tag="ON345_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR3071504.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(9056..12598)
                     /gene="recB"
                     /locus_tag="ON345_001420"
     CDS             complement(9056..12598)
                     /gene="recB"
                     /locus_tag="ON345_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR3071505.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGAALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12591..15479)
                     /gene="ptrA"
                     /locus_tag="ON345_001421"
     CDS             complement(12591..15479)
                     /gene="ptrA"
                     /locus_tag="ON345_001421"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR3071506.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISSKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FNYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(15655..19023)
                     /gene="recC"
                     /locus_tag="ON345_001422"
     CDS             complement(15655..19023)
                     /gene="recC"
                     /locus_tag="ON345_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR3071507.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSAIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19036..19359)
                     /locus_tag="ON345_001423"
     CDS             complement(19036..19359)
                     /locus_tag="ON345_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR3071508.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19344..19751)
                     /locus_tag="ON345_001424"
     CDS             complement(19344..19751)
                     /locus_tag="ON345_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR3071509.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(19748..20311)
                     /locus_tag="ON345_001425"
     CDS             complement(19748..20311)
                     /locus_tag="ON345_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR3071510.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(20302..20772)
                     /locus_tag="ON345_001426"
     CDS             complement(20302..20772)
                     /locus_tag="ON345_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR3071511.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(20957..21751)
                     /gene="thyA"
                     /locus_tag="ON345_001427"
     CDS             complement(20957..21751)
                     /gene="thyA"
                     /locus_tag="ON345_001427"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3071512.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(21758..22633)
                     /gene="lgt"
                     /locus_tag="ON345_001428"
     CDS             complement(21758..22633)
                     /gene="lgt"
                     /locus_tag="ON345_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR3071513.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(22784..25030)
                     /gene="ptsP"
                     /locus_tag="ON345_001429"
     CDS             complement(22784..25030)
                     /gene="ptsP"
                     /locus_tag="ON345_001429"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3071514.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(25043..25573)
                     /gene="rppH"
                     /locus_tag="ON345_001430"
     CDS             complement(25043..25573)
                     /gene="rppH"
                     /locus_tag="ON345_001430"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR3071515.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         6163 a         7181 c         6463 g         6015 t
ORIGIN      
        1 gtttgccgtc agagacgatg gtacgtccgt cattggtgtg catcacatag ttcgaaccgg
       61 aacaggcgct cagggcaaaa gtcagcatac aggcagaaat aattgcggca gtctttttca
      121 tcatcttctc cttaaagcga ttaataaatc ttaatagtat tgattaacag gctaaaatta
      181 acgcctaaca ctattcagca tatgttactt acgcggcttc gccaggatat ccagataatt
      241 ctgatggtta gcactctcct tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag
      301 ccgaggagta ccatgaacgt ttttaatccc gcgcagtttc gcgcccagtt tcccgcacta
      361 caggatgcgg gcgtctatct cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt
      421 gaagccaccc aacagtttta cagtctgagc gccggaaacg tccatcgcag ccagtttgcc
      481 gaagcccaac gcctgaccgc gcgttatgaa gctgcacgag agaaagtggc gcaattactg
      541 aatgcaccgg atgataaaac tatcgtctgg acgcgcggca ccactgaatc catcaacatg
      601 gtggcacaat gctatgcgcg tccgcgtctg caaccgggcg atgagattat tgtcagcgtg
      661 gcagaacacc acgccaacct cgtcccctgg ctgatggtcg cccaacaaac tggagccaaa
      721 gtggtgaaat tgccgcttaa tgcgcagcga ctgccggatg tcgatttgtt gccagaactg
      781 attactcccc gtagtcggat tctggcgttg ggtcagatgt cgaacgttac tggcggttgc
      841 ccggatctgg cgcgagcgat tacctttgct cattcagccg ggatggtggt gatggttgat
      901 ggtgctcagg gggcagtgca tttccccgcg gatgttcagc aactggatat tgatttctat
      961 gctttttcag gtcacaaact gtatggcccg acaggtatcg gcgtgctgta tggtaaatca
     1021 gaactgctgg aggcgatgtc gccctggctg ggcggcggca aaatggttca cgaagtgagt
     1081 tttgacggct tcacgactca atctgcgccg tggaaactgg aagctggaac gccaaatgtc
     1141 gctggtgtca taggattaag cgcggcgctg gaatggctgg cagattacga tatcaaccag
     1201 gccgaaagct ggagccgtag cttagcaacg ctggcggaag atgcgctggc gaaacgtccc
     1261 ggctttcgtt cattccgctg ccaggattcc agcctgctgg cctttgattt tgctggcgtt
     1321 catcatagcg atatggtgac gctgctggcg gagtacggta ttgccctgcg ggccgggcag
     1381 cattgcgctc agccgctact ggcagaatta ggcgtaaccg gcacactgcg cgcctctttt
     1441 gcgccatata atacaaagag tgatgtggat gcgctggtga atgccgttga ccgcgcgctg
     1501 gaattattgg tggattaatg acaaacccgc aattcgccgg acatccgttc ggcacaaccg
     1561 taaccgcaga aacgttacgc aataccttcg caccgttgtc gcaatgggaa gataaatatc
     1621 gccagttgat catgctgggg aaacagcttc cggctttgcc agacgagtta aaagcgcagg
     1681 ctaaagagat tgccggatgc gaaaaccgcg tctggctggg atataccgtg actgaaaacg
     1741 gtaaaatgca cttctttggc gacagcgaag ggcgcattgt gcgcggcctg ttggcggtgt
     1801 tgttgactgc cgttgagggg aaaaccgccg ccgaattgca ggcacagtcg ccgctggcat
     1861 tgtttgatga gctgggattg cgtgcacagc ttagcgcctc acgcagccag gggttgaatg
     1921 cgttaagcga ggcgattatc gctgcggcga agcaggttta agcacagaac caggtcggat
     1981 aagacgttcg cgtcgcatcc gacctgattg tttaaccctg acgcgccgct ttcgccatca
     2041 tcttcttcag cgcatgagaa accgcaacaa acccaaaggt ggcggtcacc atcgttgccg
     2101 cgccaaatcc agatgcacaa tccatccgct tcggcccttc tgccgtggct ttcatcgcac
     2161 acacagtacc gtctgactgc gggtacacca gcgcttcagt agaaaacacg caatcgacgc
     2221 cgagcttacc tttactgttt ttcactacgc caaaatcgct tttcaggcgc tcgcgcaact
     2281 tcgccgccag cggatcctga atcgttttcg ccagatcggt aacctgaatc tgcgtcgggt
     2341 caatctgccc acccgcgcca ccggtcgtga ccagcgggat tttattgcgc cgacaataag
     2401 caatcagcgc cgctttgggc cgtacactat caatggcatc aatcacgtac gaataaccca
     2461 cgctcatata ctgcgctacg ttatccggcg tcacgaaatc atccaccacc gttacacggc
     2521 actccgggtt aatctggcga atacgctccg ccatgacttc cgcttttgcc agcccgacgt
     2581 tatcgcgcag agcgtgaatt tgccgattgg tattggtgac gcacacatca tccatatcga
     2641 taagcgtgat tgcgccaatc cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac
     2701 caccgatacc aaccacacaa atatgcgcgt cagcaaacag ttgcaacgct ttttcaccat
     2761 acagacgcgc tgtgccacca aaacgctgac gccatgcatc actaattacc acagacatag
     2821 cacctcagaa ttaaaaaggg tgaggttatc cctcaccctg tcgtatccgt aaaaacggta
     2881 tacagaataa cacatcagcc gctaaagacg ttacctgcgc ccggggcggt tttcagcacc
     2941 cagacacgtc catagtggtt gtaccaacct gcgcgatgtc cggcttccgg cccgatccct
     3001 tgatagatat cgaagtgttg gcctttgatt gcgccaccga catccagcgc caccatcaga
     3061 cgcaattcgt actgaccgtt aaatttgccg ttattatcca gcagcggcac ttctgccagc
     3121 aaggtagtac ctggcggaat aattgaacga tcggaggcaa cagaagcacg gccaaccagc
     3181 ggcaccgcac ttgccccttt caccggtgca aaagattgcg gtttaaagaa gacgaaagac
     3241 gggttctgtt ccagcagctc gcgaacctcg gcttcactgt gtgtttcgcc ccaatgacga
     3301 atcgcctgca tcgacatatc ttcttttttc acttcgccac ggtcgatcag caccttacca
     3361 atgctgcgat aggcatgacc gtttttccct gcatagctga aaaagttaag cggactgcca
     3421 tcaccaaagt cgatataccc gctaccctgc acatccataa tgaagttatc catcagggag
     3481 ttgctgtaag cgagaatata tttatcactc agcgcccctg cgtagatctc cgcacgagac
     3541 ggcagacgac cacgttttgg cggcatacgg taaataggat actggaactc gccctggcgg
     3601 gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa actgcacgtt accatagttg
     3661 tcggcacctt ccatctgcca ggcatcaatg ccgaactgac gcatattgcg ggtatcaccg
     3721 cctgcgcgca gccactcttg caccgcgtta taaacattac tctggttgcc atacagacgc
     3781 ggtgacgaat tacggatatg gttaatttgc tcggcaaaat caccggcgtt aatcggcgcg
     3841 ccaacggcat ctggctggtt caccagagag aaaggctggg taaatttccc gtctttatat
     3901 tgctgtccgc gatcggttgg tttggaagag caggcggcaa gcattgccac aaccgtgccc
     3961 ataagaaggt actttaccca acgtcctttc attgttctct tcttcaggtt aaaaaataag
     4021 gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga gcagcctctc cctgacgcaa
     4081 attttgcaca aaaaataggc tttagtgatt tgtttttgtt caaaatcatg ccaaatccgt
     4141 gatcggggta aaaaaaaggt tgcatgaaaa cgcgagcgga gtatagtgcg catccacgga
     4201 cgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg tcggttcaaa
     4261 tccggccccc gcaaccaatt attgaacacc ctaacgggtg tttttttgtt tctgctctcc
     4321 catataaaaa gcgccattca gcgccttttt atcatcccct tctcgccagc gtcgccccat
     4381 cggcaaaata cgctttaatt cccgcaagaa tagactccgc aacttcctgc tggaaagtcg
     4441 ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg accagaatgg
     4501 agggaatatc aggcgctttc agcacggcaa acccggcctg ttcaacctga tttttatgca
     4561 gcttgttgat tttaccgagc ttattcagta ccgctttacc aaacttcagg ctgtcggcaa
     4621 tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc
     4681 tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt gccgcagtac
     4741 tggttgcacc tttggttgag agcgcaaaca cagaggaacc gctcggctga cgactggtaa
     4801 aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct tttgctacgc
     4861 gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc atattgccct
     4921 ctttctcgat cagagagcgc aaacggcgag ctatttgcaa taccacgtct ttttcgcgcg
     4981 ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca agcataatga
     5041 caatcggacg atcgcgccct gctttacctg gttgtggacc actttgcgct ggcggcacct
     5101 gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat
     5161 cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg gcgaccggcg
     5221 caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatc cgtacggttt
     5281 gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg
     5341 ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc accacgcgtt
     5401 ccgggttact caacgcgaac tgcttatatt tcagctgacg atttgattct accgtcacac
     5461 gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctgacc gcagccagac
     5521 tgacctgact tacgctcaat agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc
     5581 tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa
     5641 caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct gtcatctaat
     5701 aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg ctctcgcata
     5761 aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acactaattt cgaataatca
     5821 tgcaaagagg tgtgccgtgg taaaggaacg taaaaccgag ttggtcgagg gattccgcca
     5881 ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga
     5941 agccattgag catgagaatt tctccagtat cgttaatgat atcgggttat tgcacagcct
     6001 cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa atctggctgc
     6061 gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca aaacactgga
     6121 actggtgaag caggctgcgg gaacattgca actggatatt actgctcgcc tgtcgatgag
     6181 tctcaataac acgccgctgc agggcgcgca tatcaacgtc gtcagtggca attttattat
     6241 tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc gtatccggcg
     6301 gattgatgaa gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc taatggggcc
     6361 ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga ttgccactca
     6421 actagccatc aaactgaaag ctgaaaagat gattggtttt tgctcttccc agggcgttac
     6481 taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag cacgggtaga
     6541 agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc gtggcgcagt
     6601 gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg aagatggcgc
     6661 gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga tggaaagcgc
     6721 tgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt tgattcgccc
     6781 gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga tggaaatcga
     6841 caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc tctatccgtt
     6901 cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt accgcagctc
     6961 atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga gcggcttaaa
     7021 caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg gatttacccc
     7081 ggtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc agcgtaaatc
     7141 caaagtgttg atggcggatt tagggtaagc gatgaaaatt gtcggatgcg acatgcgtaa
     7201 cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa cgccgccaga
     7261 ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc atcggcatac
     7321 agcgacagac ggcgacgcgc gcgggtcacc gcggtgtata ccagctctcg cgttactacc
     7381 ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta
     7441 tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg cacagactta
     7501 atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata
     7561 ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcga
     7621 ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg cataaactgc
     7681 tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc gcacaaaagt
     7741 tggtactcat tgaacgcctg aatgattaaa tccggctcag cacgcgcttg cagcagatcc
     7801 agataacgtc cataacccgc aagagcttcc tcaagcatcg caatataatc ttcgccgctc
     7861 tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt
     7921 ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga
     7981 tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccggttcct
     8041 gccggaacgt gagtccccgt caggcggctt agctgcccgg cacgctcggc ggtaaagccg
     8101 gcattggcat aagcgcagat atcgcccagc acagccccag cctcaaccga ggccagttga
     8161 tcacgatcgc cgagaaagat cactcgcgca tgatcgggca aggcgtcgat cagtctcgac
     8221 atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag atgcagcggg
     8281 ttaccggcat gatgacgtaa acgctggcta cccggctgcg cacccagcaa tcggtgcaaa
     8341 gtactggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa
     8401 gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg
     8461 atacggcagc gttcgccgtc ggccatttga attaacgctg ccagtaactt cgccacggtg
     8521 gtcgttttac cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt cagcgccact
     8581 gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt gtccagggtt
     8641 tgcgccagta gagcttcatc aacctcaatg gcatgattca cctcattgaa gaagcgcgct
     8701 accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca gaggatcatc
     8761 ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc ttcccaattt
     8821 tgtagctcac cgatttcact gacacaggtc gccaacagcg gatgcgacgc ctcgttattt
     8881 tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact taacagtgcc
     8941 gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa ttgcacatcc
     9001 agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa tttcattacg
     9061 cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac ccggcgttgg
     9121 gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc aggaacagat
     9181 agataacgcc gccaaagtga cgatcatagt cgtaatcagc aatgcgatgg cgcagataac
     9241 gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcatcgctg
     9301 ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg gatttatagt
     9361 cgagcaggta ataacgccct tcgtggcgga ataccaggtc gataaagcct tttaacatgc
     9421 cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca aactggcgaa
     9481 ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa aactccatct
     9541 ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccgtttca ttgagaggtg
     9601 cctggaggac agccgtgatc cactcggtca acaccggttc ccactgcgat tcaaagccgc
     9661 cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt
     9721 caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga tgcggtgtta
     9781 acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca
     9841 gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg acacgccagt
     9901 tatcgccggg caatcgttgt agcgtcctcg cattcagttc tgcagttaaa gcatcattaa
     9961 cctgccaggg ctgattatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata
    10021 acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac
    10081 gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca
    10141 gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac
    10201 gcagatcttc cgccagacgt tcgacctccg caagctcgac gctttctggc gcagcgttaa
    10261 gatccagaac agcctcaaac gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga
    10321 aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat ttatggatcg
    10381 tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac
    10441 tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct
    10501 gcgttccggc ttcttgtagc agttcgctga tatgcaagat atcggtaaga cgccgctcac
    10561 cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca
    10621 tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct tcaactaccg
    10681 catcccacgc atgttcgtca ttattcagcg tttcgatatc cagcgcgttc agccccatca
    10741 ttgacgttgc cagcgcacta cgcagggtgt tctcacgttc gggcgtcatc accgcctgca
    10801 acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt
    10861 aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc tcctggcggc
    10921 tgcgcaccag cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca
    10981 gcaacgctgc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca gcacatacct
    11041 gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc agccacattt
    11101 tcatcgcagg ctgcgtttca cctttaaaca caaaacgtaa cgcctgattt ttcccggctg
    11161 atttcactgg aataaacggt atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa
    11221 gcttgttcac actgttcacc attcctggtg cggaacgcca gttggtgtct aaagtgtagt
    11281 gggcgtgaac ttcgctacgc gccttcatat aagtgaagat atccgcaccc cggaatgcat
    11341 atatggcctg tttcgggtcg ccaatcagca acaatgcggt ttccggctga tggtgccaga
    11401 tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatca atcattgcca
    11461 ccgggaatcg cgtacggatc gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg
    11521 aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg
    11581 ctactgtttc gcggatctca gccaatgcgc gggttatcac cagatcacgg atcgacaatg
    11641 gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccgg
    11701 ctttcgtgcg atcttctaag aaacgctgag agaatttttc cagcgactcc ggtaactgat
    11761 aactgttggt ctcttcttct gcccaggcgc tgatcttctc gatccattta gcctgattgc
    11821 tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca
    11881 ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc gcgtggcggg
    11941 aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggtgcttcg ccttgcagat
    12001 aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag accaccaggg
    12061 caatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtagc
    12121 gtagcagaga ctcatcttca atcagctgct gctcaaacag cataccggat tcaaaggcat
    12181 tcaggttgag catgcgctgg caaaaaccgt gaatagtaaa gactgccgct tcatccatct
    12241 gccgttcggc taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca
    12301 ggcgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga
    12361 tattgctacg gatacgacca cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca
    12421 gcagttcttc aacggtcagc gggcggggaa aggcggcgga accgcctagt ccaagtaaca
    12481 ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca
    12541 ggcgctcacc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc
    12601 ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag
    12661 ccttcagggt gtacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata
    12721 gccatgcctt gcggctcgac caccgcctga tggaaaaaat cagcaagttt ttgcggcgtc
    12781 agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg
    12841 aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg
    12901 gtaattaccg cctgctggat ttgcgcaaac tcttctggct tcatcgcccg caattttgcc
    12961 tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg
    13021 ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg
    13081 gcatagccca actgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc
    13141 cccaacagtg agctataggc tgagctggtg tattcatcgt agccagtcgg tacaaatacc
    13201 gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga ttgtttttta
    13261 tcgaccacga catctttgtt acgacaccac tccgaaccat cagcgcccaa ctgtttttgc
    13321 acatcgcgtg ccagcgttgt tgcctgggct tcggtcatgt tgccaataac cataaactct
    13381 ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa cgtaatggag
    13441 ggcaaaattt tgcgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg
    13501 ggcataatcg cctgctcgaa cgctttgccc ttttctgcgg aatccatcat ctggttatac
    13561 caggacttcg cctgctcaag ctgatcttcc gtagcggtat agttaaagta gccctcgagc
    13621 aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaac cataaggccg
    13681 ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct taactgatca
    13741 agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc
    13801 atggctttcg gattacgcaa aatcaggctg acatcggctt tgggctcgct ggcaaaataa
    13861 cggcttggcg catacactac tcgcagattc gactcatcaa caatcagctc tggatggtcg
    13921 tatttcttct ctgacttaat cagcgagaaa tcgtcaggaa tataagggtt aagctctggc
    13981 aaagagagcg caatgtcggc agcttttttc tgccagtcag cgaaagtttg ttcgctgatt
    14041 ttatcgacct gatacggcgc atcgacaaaa taagccgttt tattgtgcgg ctctttcgag
    14101 ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc ctttactgct
    14161 ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac aggaacgcga
    14221 atcatggtat ctgccaacca ttcgacgtaa tccatatcac gggtgatcga cggataacgg
    14281 aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcaat gcctttttca
    14341 cgtaacagat tgagatagct aaaaattgcc gccacaacct gatcgcgatt agccaggcct
    14401 ttatcggtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg
    14461 gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcgga aagtgtacct
    14521 gggctgcgat taccaatcag ataggtaatc aattcatccg ttttactacg gaacttcgct
    14581 gagttgttat cgatgcgaaa ctcaacgcgc agaactttac gcggcagtgc cgggacgtaa
    14641 tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg
    14701 ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa ctccggcagc
    14761 ggtttattgc tgtaaataac cgccttcatc agattggcgg aatagtactt ctcgtggaaa
    14821 tctttcagcg cctgctgcac cggattacca ggtttatcgc ttaaagtttc gaggttacca
    14881 ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg
    14941 cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcaccgcatt acgctcacgt
    15001 tcggcatatt tcttgtcgag caaaggttca gcaatagcat cggccaggcg gtctaccgca
    15061 ccaggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg cgcagtgctg
    15121 gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac
    15181 tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg
    15241 ggatcttcca gcgacccaac gggcaccacc agcgccgaga gcgatttaac tgcctgcgga
    15301 tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta
    15361 tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt
    15421 aagggtgccc aaagggcaac taacaacaat aatgctttga accaggtgct gcggggcatt
    15481 cacggacctc ataatcaact taattttctg tccagattca acacgttaac gaaaatcatc
    15541 tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc atcagtccgt
    15601 gactggtgcg catcataaag taagcggata gattgcgcaa tttttataca gcactcatga
    15661 ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga tggcctccat
    15721 tgtctctggt gttaattgcc gccagagcct ttgataccag atatcatcac cttcgccacg
    15781 caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gcaacgtgga
    15841 atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc
    15901 actttcaggt aacaccagca atggcgcgga cattccttca cgatacccct caatcagttg
    15961 tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact cgccgtcttt
    16021 gcgtagaaaa aggcgacttt cgccattacc accgctggca cagtagacaa ggtgttccag
    16081 ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca acaggccatc
    16141 cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga gatcaatttc
    16201 catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct gcatctcctg
    16261 gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg
    16321 gaagcggcgg aacaagcgtt cggcatcatc ctgttcaacc agtgcattca ataactgctg
    16381 attgatttga taacggctaa gtccttccag aataaatggc tcggtgtctg ggattgcgct
    16441 gtcttcagta cggaagttca cctgcaaacg catctggaag aacgcccgca ccggatgtgc
    16501 ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg
    16561 aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata
    16621 gctttgtcgt tcgcctggct ggtagttttg tggatcaaac ggcatccggg tatggaggca
    16681 agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg
    16741 tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg
    16801 ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt gctgcgcgga
    16861 aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt cgccacgctt
    16921 cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc
    16981 gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca tcagagtaca
    17041 aatgttaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca gacgctgcgc
    17101 cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc
    17161 ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc
    17221 cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt ctagcggacg
    17281 ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc
    17341 caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt gccactcgcc
    17401 ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc gccaggtgtg
    17461 ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat
    17521 gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg
    17581 cgccgccagc accggcacat ccagcagcgc cagcacatcc tctgacacaa agcgactgtc
    17641 aggcagtgat aacaggctga taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg
    17701 gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat
    17761 aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg gagtaagtgt
    17821 cgggtcttcc tccagcatcg ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg
    17881 gctatggcaa acgtggaatg tgatactgct atccagtgga tcaagcgggc gtttgttatc
    17941 gctacgggaa aactcttcga tgttcacacc cgcaacggcg cggttttcca gttccagaat
    18001 gtcagactga atgttatgca gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc
    18061 ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct tcccccatga
    18121 ggccagcagc gggttgccga catcctgttc accatcgctg ttaaagagct gtccggcatt
    18181 ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt
    18241 cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac ggcaggggtt
    18301 ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct ggagataaac
    18361 aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt
    18421 cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg
    18481 ttgtccgagt tcatgggtat attccaccag cgccttccac aacggggctt gccaggcctg
    18541 tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg
    18601 atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga acagttttcg
    18661 cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgc gctccagcaa
    18721 ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt
    18781 ggggatttcc ggtaacaccc ggacgaacat atcccagata aagcttgctg gcagcggaaa
    18841 atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc actgcgccat
    18901 accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc
    18961 gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa
    19021 catagcggct cctgactact gacgattcgg gcaatgcagg cgggtcatct cgccttctct
    19081 gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcat
    19141 tcggttgacc tgccagttgg caggtggcga aatcgcgcgc agttgcgttt gctgccaacc
    19201 atgccgccag agctgttggt actggtttct gctggcgaga ctgttcatta atgttcgctg
    19261 gatacccgat aacgcagtga caatcatcac catcagcacc atcgcaacca ttacctccgg
    19321 caggctaaag ccttgttgat tcttcaggga agctgacata atgccccctc tttcagcgga
    19381 caaaaatcgc tccagccgcg tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt
    19441 cgccacaacg aaacgccttc ataaccggca atcaataagg cttcattatc tgccagtaaa
    19501 cgcaaacaaa cctgggcatc ggttccagcg tactgcgagc actgaactgc tggctgcgtc
    19561 tgccaggagt gcatttttcc ccacgccagc gccgactgaa cgatggcctg gcggcgcaat
    19621 gactgacttt ccatgctcac gcgagaggca aaactgcgat cctgctgact cattccttgc
    19681 aatagcaagc tacccaaaac cagcagcatc aggaccagag ccagtgacga aacacctttt
    19741 tcgcggttca caggttgaat cctgtcacgc tatagctggc atccaccacg gtttgcggtt
    19801 cagacttact ggcagcacgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct
    19861 gacgtacgac ctgaaaagtg tcgataatga tggcatccgg attagtgact ttatcccagc
    19921 ccttaccttc acaggatgtc gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa
    19981 atccaatctg gtcagactct tttaccggtt cgcgatccca gataccgtta ctgttcgcat
    20041 cccactgcac aatgatacag tcaccctgtc cgacaatttc cagcccttcg ccggtacagc
    20101 tgccatgaca ataacccgcc ctctggagat gcttcgcgac ggtaaatacc cgcagccaga
    20161 tttcatcttc cagcgccagc ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca
    20221 gaaagcgtgc agcccccagc aacaatacgc tactgatcgc catagcaatc aacacttcca
    20281 gcagagaaaa accttgctct tttacaggca tccttctgtt tctccttgct gacaaagccg
    20341 gagtcttccc cacggcgaaa ccaccagcca ccactcgccc gttgagtttt tgaagcgaat
    20401 atgcccggcc catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct
    20461 catttcgact tcgggccagc gtggcacaaa gaccaatggt gaactgccat gacaggtatt
    20521 ggccccagca gcggaactca caaggcacca taacgtcccc tccctgataa cgctgatact
    20581 gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag agcaaatagt cccgcgcctg
    20641 gctggcggtt tgccatagcc gttgcgactg ctgccagtat tgccagccat agagtccact
    20701 tgcgcttagc atgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg
    20761 ttgtgttttc atgccggcag tatggagcga ggagaaaaaa agacgagggc cagtttctat
    20821 ttcttcggcg catcttccgg actatttacg ccgttgcagg acgttgtaaa atttcgggaa
    20881 ggcgtctcga agaatttaac ggagggtaaa aaaaaccgac gcacactggc gtcggctctg
    20941 gcaggatgtt tcgtaattag atagccaccg gcgctttaat gcccggatgt ggatcgtagc
    21001 cttcaatctc aaagtcttcg aaacggtagt cgaagatgga ttcgggttta cgtttgataa
    21061 tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga tccatatgat
    21121 tgctgtacag gtgcgtatcg ccaccggtcc agacaaaatc acccacttcc agatcgcact
    21181 gctgcgccat catatgcacc agtaacgcgt agctggcaat gttgaacggc aggccgagga
    21241 agacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc acatagaact
    21301 ggaagaatgc atggcacggt gccagcgcca ttttatccag ttcgcctacg ttccacgctg
    21361 aaacaataat gcggcgggaa tccgggtcgt ttttcagctg gttcagtacc gtagtgatct
    21421 ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca ctgtttacca tacactggtc
    21481 cgaggtcgcc gttttcatcg gcccattcgt cccagatggt gacattgttt tcgtgtagat
    21541 aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc gtggatgatg gaacgcaggt
    21601 ggcaacgttt agttgtcacc agcgggaatc catcctgcag gttaaaacgc atctgatgac
    21661 caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca
    21721 ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt tgctgtgggc
    21781 tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg atggaaagaa
    21841 tttgccccat gctgatgtac tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga
    21901 aaaactcaac aatgatgcga aacgcgccgt agccaatcag gaacaagcca gagacagctc
    21961 ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcacc acaccttcca
    22021 gcagcagctc gtaaagctgt gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg
    22081 attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag cctgggaaca
    22141 gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc
    22201 ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca gaaacctgga
    22261 agaaggaacg tttagtacgg cgggcaaaga taatcatcac cacgataacg ccaatcaggc
    22321 cgccgtggaa agacatgccg ccgtcccaga cacggaacag atacagcgga tcggccataa
    22381 actgcgggaa attgtagaac agaacataac caatacgtcc cccgaggaag acgccgagga
    22441 agcccgcata gagtaagttt tcaacttcat ttttggtcca gccgctgccc ggacgattcg
    22501 cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc aggccgtacc
    22561 agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga aaatgcagat
    22621 agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga acagcgattg
    22681 aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca aggggacctg
    22741 gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac cctccgcgaa
    22801 tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatgacga acttcggtcg
    22861 ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcaaaa tcaatgcgcc
    22921 gcagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg cgatacccaa
    22981 gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac aaacggagat
    23041 cgataccatg tatttccgct tcccgggcga tcatcgccag agctcgtaac attgcaggat
    23101 gaagactgtc ataaatgttc gccacccggg tattgttgcg atcaacggcc agaatgtatt
    23161 gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga tgcggcagca
    23221 taaataccat tgacggcact tccagcatga tgccgatacg tggtttggga atttcgtaac
    23281 cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct tcatcaactt
    23341 catcgaggct tgtgaccatc ggcaacagaa tattcaggtt gcccgtagcg gcattagcac
    23401 gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta atgcgaatcc
    23461 cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg
    23521 ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc agcatcccct
    23581 gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg aatgggattt
    23641 cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcttca tgttccgggc
    23701 ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactgc gcgggtaaat
    23761 tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc cgctgatatt
    23821 cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca tcaacgatca
    23881 gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta gggatcccca
    23941 gcgcacgtac catgatcgca gcatgggagt tggctgcgcc atctcgcacg acaacaccga
    24001 ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct gccaccagaa
    24061 tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga aacagcaatc
    24121 gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg cttagcgcgg
    24181 caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc accgagcctt
    24241 tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagtagg tgcgagtaaa
    24301 gatcgaaaat agccgccgtt tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc
    24361 gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc agagccggat
    24421 ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt
    24481 cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg cgatattgcc
    24541 caaacaaggc agtcaactgc gactgagaaa gaatagctgc catctgggtg gcaagcgtca
    24601 ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt accaccagta
    24661 caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt
    24721 cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg
    24781 gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc gttacagtgc
    24841 gaccgcgagg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat
    24901 cggccaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg gtaaccagaa
    24961 tatttaacgc ctcattcagg cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc
    25021 gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat gcgttttgtg
    25081 gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc attaccctac
    25141 ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa cttacccatc
    25201 gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca tcgccgctta
    25261 ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc gtgtcccaac
    25321 gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca aggattcgaa
    25381 cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc tgctctgcgg
    25441 attctccggg gttgattccg ccttgcggaa attgccagga gtgctgacca aatcgccggg
    25501 cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg cggtagccat
    25561 cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat tgtttcatac
    25621 tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca attgaataac
    25681 tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt ttattcacct
    25741 tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc ggagcattct
    25801 gaataagccc gatatagaca tt
//
LOCUS       DAJSKJ010000040        25808 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000040 DAJSKJ010000000
VERSION     DAJSKJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.040"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ON345_001431"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ON345_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3071516.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ON345_001432"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ON345_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3071517.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ON345_001433"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ON345_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR3071518.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ON345_001434"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ON345_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR3071519.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ON345_001435"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ON345_001435"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR3071520.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ON345_001436"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ON345_001436"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR3071521.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQYWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ON345_001437"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ON345_001437"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR3071522.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ON345_001438"
     CDS             complement(4598..5320)
                     /locus_tag="ON345_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3071523.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ON345_001439"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ON345_001439"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3071524.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ON345_001440"
     CDS             6520..6738
                     /locus_tag="ON345_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR3071525.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ON345_001441"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ON345_001441"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3071526.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCSGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ON345_001442"
     CDS             complement(7472..7672)
                     /locus_tag="ON345_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR3071527.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ON345_001443"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ON345_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR3071528.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGGLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ON345_001444"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ON345_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR3071529.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSSPALLKEWLDRVLSRGFASGSGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="ON345_001445"
     CDS             10169..12082
                     /locus_tag="ON345_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3071530.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQDTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="ON345_001446"
     CDS             12082..13104
                     /locus_tag="ON345_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3071531.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYKLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="ON345_001447"
     CDS             13098..13316
                     /locus_tag="ON345_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR3071532.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="ON345_001448"
     CDS             13370..14239
                     /locus_tag="ON345_001448"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR3071533.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="ON345_001449"
     CDS             complement(14294..14698)
                     /locus_tag="ON345_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR3071534.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ON345_001450"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ON345_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR3071535.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="ON345_001451"
     CDS             15683..17773
                     /locus_tag="ON345_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3071536.1"
                     /translation="MWHRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="ON345_001452"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="ON345_001452"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR3071537.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ON345_001453"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ON345_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR3071538.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDKAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /locus_tag="ON345_001454"
     CDS             complement(19741..20343)
                     /locus_tag="ON345_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR3071539.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="ON345_001455"
     CDS             complement(20333..20500)
                     /locus_tag="ON345_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR3071540.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ON345_001456"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ON345_001456"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR3071541.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ON345_001457"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ON345_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR3071542.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ON345_001458"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ON345_001458"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR3071543.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ON345_001459"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ON345_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR3071544.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6302 a         6977 c         6452 g         6077 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagtactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaggaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcaac cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gctgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgcacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgctc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gcccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccatggtt tttcgtcttc ttcctccggt ccgttaaact
     8341 ggcgggatag ccatttatct accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc cccccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac cgccagatcc tgaaaaagca gtaccgagaa acctaactgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccgacccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggctgcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcaggaca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagttcgaag gcgcgctggt tgtcgtctcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgacctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 cgcgccaaaa gagaatgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcaacttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcatccc catgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct tgcgtggagt gaaaaccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaaca gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta taaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaatagcctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac aaagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagtgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggca cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gtactgtggt ttcagctctg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcccccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtaacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaacaaa tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggctttat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccacgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggct tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagtcc
    19921 gcttcccaca cggaagggat gcagcacatt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggattccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtatcg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag cacctggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca ggggatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagaacgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatga
//
LOCUS       DAJSKJ010000041        25519 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000041 DAJSKJ010000000
VERSION     DAJSKJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.041"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            224..643
                     /locus_tag="ON345_001460"
     CDS             224..643
                     /locus_tag="ON345_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR3071545.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            643..1911
                     /gene="umuC"
                     /locus_tag="ON345_001461"
     CDS             643..1911
                     /gene="umuC"
                     /locus_tag="ON345_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR3071546.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1957..2487)
                     /gene="dsbB"
                     /locus_tag="ON345_001462"
     CDS             complement(1957..2487)
                     /gene="dsbB"
                     /locus_tag="ON345_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR3071547.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(2633..4174)
                     /gene="nhaB"
                     /locus_tag="ON345_001463"
     CDS             complement(2633..4174)
                     /gene="nhaB"
                     /locus_tag="ON345_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR3071548.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4396..5115
                     /gene="fadR"
                     /locus_tag="ON345_001464"
     CDS             4396..5115
                     /gene="fadR"
                     /locus_tag="ON345_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR3071549.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5167..6699)
                     /locus_tag="ON345_001465"
     CDS             complement(5167..6699)
                     /locus_tag="ON345_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR3071550.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7029..8327
                     /gene="dadA"
                     /locus_tag="ON345_001466"
     CDS             7029..8327
                     /gene="dadA"
                     /locus_tag="ON345_001466"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR3071551.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8337..9407
                     /gene="dadX"
                     /locus_tag="ON345_001467"
     CDS             8337..9407
                     /gene="dadX"
                     /locus_tag="ON345_001467"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR3071552.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9794..11530)
                     /locus_tag="ON345_001468"
     CDS             complement(9794..11530)
                     /locus_tag="ON345_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR3071553.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11625..12539)
                     /gene="ldcA"
                     /locus_tag="ON345_001469"
     CDS             complement(11625..12539)
                     /gene="ldcA"
                     /locus_tag="ON345_001469"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR3071554.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12639..13250
                     /gene="emtA"
                     /locus_tag="ON345_001470"
     CDS             12639..13250
                     /gene="emtA"
                     /locus_tag="ON345_001470"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR3071555.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13252..13986)
                     /locus_tag="ON345_001471"
     CDS             complement(13252..13986)
                     /locus_tag="ON345_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR3071556.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14187..14441
                     /locus_tag="ON345_001472"
     CDS             14187..14441
                     /locus_tag="ON345_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3071557.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14619..15059
                     /locus_tag="ON345_001473"
     CDS             14619..15059
                     /locus_tag="ON345_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071558.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15138..16835)
                     /gene="treA"
                     /locus_tag="ON345_001474"
     CDS             complement(15138..16835)
                     /gene="treA"
                     /locus_tag="ON345_001474"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR3071559.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAKETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDRENKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17155..18573)
                     /gene="dhaM"
                     /locus_tag="ON345_001475"
     CDS             complement(17155..18573)
                     /gene="dhaM"
                     /locus_tag="ON345_001475"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR3071560.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18584..19216)
                     /gene="dhaL"
                     /locus_tag="ON345_001476"
     CDS             complement(18584..19216)
                     /gene="dhaL"
                     /locus_tag="ON345_001476"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR3071561.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMSDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19227..20297)
                     /gene="dhaK"
                     /locus_tag="ON345_001477"
     CDS             complement(19227..20297)
                     /gene="dhaK"
                     /locus_tag="ON345_001477"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR3071562.1"
                     /translation="MKKLINDVRDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20525..22444
                     /gene="dhaR"
                     /locus_tag="ON345_001478"
     CDS             20525..22444
                     /gene="dhaR"
                     /locus_tag="ON345_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR3071563.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLLSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSNLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22544..25411)
                     /locus_tag="ON345_001479"
                     /pseudo
     CDS             complement(22544..25411)
                     /locus_tag="ON345_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         6307 a         6340 c         6571 g         6301 t
ORIGIN      
        1 aatgtcatta tggcgaatgc ttctattcta tttttttgcc gggtgatatt tttcatttct
       61 gctggatgag cgtcgtcgcc agaaggccac gtgagcacag gataagagaa cgaaaaatca
      121 gcagtctatg cagcgacaaa tattgatagc ctgaatcagt attgatctgc tggcaagaac
      181 agattactgt atataaaaac agtataactt caggcagatt attatgttgt ttatcaagcc
      241 tgcggatctc cgcgaaattg tgacttttcc gctatttagc gatcttgttc agtgtggctt
      301 tccttcaccg gcagcagatt acgtcgaaca gcgcatcgat ctgaatcaac tgttgatcca
      361 gcatcccagc gcgacttact tcgtcaaagc aagtggtgat tctatgattg atggtggaat
      421 tagtgacggt gatttactga ttgtcgatag cgctattacc gccagccatg gtgatattgt
      481 catcgctgct gttgacggcg agtttacggt gaaaaaattg caactacgcc cgacggtaca
      541 gcttattccc atgaacagtg cgtactcgcc cattaccatc agtagcgaag atacgctgga
      601 tgtctttggt gtggtgatcc acgtcgttaa ggcgatgcgc tgatgtttgc tctctgtgat
      661 gtaaacgcgt tttatgccag ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg
      721 gtggttgtgc tatcgaataa tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg
      781 ctgggtgtta aaatgggcga tccctggttc aaacaaaaag atctgtttcg tcgctgtggt
      841 gtggtttgct ttagcagcaa ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg
      901 acgctggaag aactatcgcc ccgcgtcgag atttacagta ttgatgaggc attctgcgat
      961 ctgacaggtg tgcgtaattg tcgcgatctg actgattttg gcagagaaat tcgcgcaacg
     1021 gtgctacaac gtacccatct tactgttggt gtggggatcg cccagaccaa aacgctggct
     1081 aagcttgcca atcatgcggc aaaaaaatgg cagcggcaga cggggggggt ggtggattta
     1141 tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga cgtctggggg
     1201 attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt tcttgatttg
     1261 gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga aagaacggtg
     1321 cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac aaagcaggaa
     1381 attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat gcggcaggcc
     1441 atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca atattgtcgg
     1501 tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta ttacggcaat
     1561 agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat taacgccgct
     1621 acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc gggcgtgatg
     1681 ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga caacgcaccg
     1741 cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa agagggcaga
     1801 ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa gcgagccatg
     1861 ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata aatatagcgg
     1921 caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca gatcacgttt
     1981 tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt aagcgataaa
     2041 aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac gctcggcgca
     2101 atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc attccgggaa
     2161 acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca tggtgtgctc
     2221 gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg ctacataacg
     2281 cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc cgaataacgc
     2341 gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct ggaaccacag
     2401 cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac aaccacgcgc cccggccttg
     2461 tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat agagtgcgca
     2521 gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac gcaattggac
     2581 aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt tatcaaagcg
     2641 ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta aactcgacgc
     2701 agagcagtcc gacgagtgtc aggacgaggg tgtaaggcaa ggccatccac accatgcggc
     2761 cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaacagg aacgcagcct
     2821 gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg accgccagca
     2881 gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct tcgttgatat
     2941 aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg aaaatataga
     3001 acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatt ggcgaaaaca
     3061 gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt gcggtgaatg
     3121 gcagagattc ggtgaaggct ttaccgatag catgttcatc ggtgacaccg gtcaatgatg
     3181 tcgccagaat aatgactgac aaaccaatca agccaacttc cgccagatgc aacgccagcg
     3241 cagtcaccag ccagacgcca ataatcgcct ggacaatcag acggatttta tcctgacggg
     3301 tacgctggtg gcggctttga tcgtcaaact gttgcaacac ttcgcggact ttctccggca
     3361 gcgtttcacc gtagccaaac caacgcagct tctctaccag caggcaggtt aacaggccac
     3421 aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca aaatgccagc
     3481 cagccgcttt agcaatgatc aggttctgtg gttcgcccac catggtcatt acgccgccta
     3541 atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg aactgttcca
     3601 gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggtg tcttcggtac
     3661 gggaagaggc tacgcgatga taaataccat aaaaaccgac tgcaacgctg atcaccaccg
     3721 ccacgacggt taaggcatcg aggaacgcgg agaggaacgc tgccgccacg caaaaagaga
     3781 gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg aacagcaaca
     3841 gctgtttcat aaaatagata cccgccacca taaacatcaa taacagcaag acttcaagat
     3901 ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat accgcttcga
     3961 tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc agagtgaaaa
     4021 taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa attaacgggt
     4081 ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg cccaaaaaat
     4141 tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc ttacaaataa
     4201 tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt gaggcaagtc
     4261 ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt ttcttctttt
     4321 tgtctgctat cagcgtagtt agccctctgg tatgatgagt ccaactttgt tttgctgtgt
     4381 tatggaaatc tcactatggt cattaaggcg caaagcccgg cgggtttcgc ggaagagtac
     4441 attattgaaa gtatctggaa taaccgcttc cctcccggga ctattttgcc cgcagaacgt
     4501 gaactttcag aattaattgg cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg
     4561 gcacgagatg gctggttgac cattcaacat ggcaagccga cgaaggtgaa taatttctgg
     4621 gaaacttccg gtttaaatat ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg
     4681 cagcttattg ataatttgct gtcggtgcgt accaatattt ccactatttt tattcgcacc
     4741 gcgtttcgtc agcatcccga taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc
     4801 gatcacgccg atgcctttgc cgagctggat tacaacatat tccgcggcct ggcgtttgct
     4861 tccggcaacc cgatttacgg tctgattctt aacgggatga aagggctgta tacgcgtatt
     4921 ggtcgtcact atttcgccaa tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa
     4981 ctgtcggcgt tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat
     5041 gggcatgaga gtggcgagat ttggcaccgg atgcagaaaa atctgccggg tgatttagcc
     5101 attcaggggc gataatccct tccgtttaaa gagcaaaccc ctcaaacgag gggttttttg
     5161 ttgtttttac agatttccca ttcttggcgg gcaacgttcc agcaactcga tgctgccgtc
     5221 ttcgttttgc tgttcgagca tcacatcaaa tccccacagg cgatgcacat gcttcaggac
     5281 ttctttgcgc ccccgatcca gcggtgcgcg attatgtgga atataacgca gcgtcagcga
     5341 acggtcgccg cgcaaatcca cgttccagat ctgaatattc ggctccagat tacttaagtt
     5401 atattgcgac gataaccggt tacggatctc ccgataacct tcttcattat gaatagcgga
     5461 aatctccaga taattatgcc gatcgtcatc cagcacggtg aagaagcgga aatcacgcat
     5521 cactttcggt gacagaaact ggctgataaa actctcatct ttgaaatcac gcatcgcgaa
     5581 atgcagcgtt tccagccagt cggaaccggc gatatccggg aaccagtatt tgtcttcttc
     5641 cgttggcgac tgacaaatcc gtttaatatc ctggaacatg gcgaacccga gggcatacgg
     5701 gttgatgccg ctgtaccacg ggctgttata ggggggctgg aagaccacat tggtgtggct
     5761 gtgcaaaaac tccagcataa aacgttccgt tactttccct tcatcataca gatggttaag
     5821 gatggtatag tgccagaagg tcgcccagcc ttcgttcatc acctgagtct gtttttgcgg
     5881 ataaaaatac tggctcacct tacgcacaat acgcaggatt tcacgctgcc atgattccag
     5941 cagtggcgca tttttctcca taaaatagag caggttttct tgtggttcgg acggatagcg
     6001 gcgcgcttca gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc agagcatatt
     6061 gacctgactt tgtagatact cttcgcgact tttctgccgg gctttctctt cttgcagcga
     6121 gattttttgc gggcgtttgt accggtccac gccgtagttc atcagcgcgt ggcacgagtc
     6181 cagaagccgt tctacttcat caacgccata acgttcttcg cactcggtaa tatatttacg
     6241 ggcaaaaatc agataatcga caatcgaact ggcgtcggtc caactgcgga ataagtaatt
     6301 gtttttaaag aaagagttat gcccatagca ggcgtgtgcc atcaccagcg cctgcatggt
     6361 aatggtgttc tcttccatca agtaagcgat acacgggtta gagttaatga cgatttcata
     6421 ggccagccct tgctgaccgt gcttatacag ccgttcagtc tcgataaact ttttaccgaa
     6481 tgaccagtgc ggatagttaa ttggcatgcc gacgctggaa taggcatcca tcatctgttc
     6541 tgaggttatc acttcaatct ggtgcgggta ggtatccagc cggtagagtt tcgccacccg
     6601 gtctatctct gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa
     6661 acgtgtggtg tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg
     6721 gcgctctctg tgtggagcac ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc
     6781 cggagaattt ttttctttgc gatttcttat tatcagagtg ccactaatcc gcttctgaac
     6841 ggaattttat gctggataaa aagggcgttc agcaggagat actaaagacg ccatattgcc
     6901 gcagagtcag ggagatgtga gccagctcac cataaaaaag ccgtatgttg aataatattt
     6961 tcaactgagt tatcaagatg taattagatt attattcttt tactgtatct accgttatcg
     7021 gagtggctat gcgagttgtc atactgggaa gtggtgtggt aggcgttgcc agcgcctggt
     7081 acttaaatca ggcaggacat gaggtcaccg tcattgatcg ggagccgggg gccgccctgg
     7141 aaaccagcgc cgcgaatgcc gggcaaatct cccccggata tgctgcaccg tgggcggcac
     7201 caggtgtgcc tttaaaagcg atcaaatgga tgttccagcg ccatgcgccg ctggcggttc
     7261 gtctcgacgg tacgcagttt cagttgaaat ggatgtggca aatgttacgt aactgcgaca
     7321 ccagccacta catggaaaac aaagggcgga tggtgcgtct ggcggaatac agccgtgatt
     7381 gcctgaaagc attgcgcgcc gaaaccaata ttcagtatga agggcgtcag ggtgggacgc
     7441 tgcaactgtt ccgtaccgaa caacagtatg aaaatgcgac ccgcgatatc gccgtgctgg
     7501 aagatgccgg cgtaccgtat cagctgctgg aatccagccg cctggcggaa gtggagcccg
     7561 cgctggcaga agtagcgcac aaactgacgg gtggtctgca actgcccaat gatgaaaccg
     7621 gagactgcca gctgtttacc cagaatctgg cgcggatggc agagcaggcg ggggttaaat
     7681 tccgctttaa tacgcccgtt gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga
     7741 agtgtggcga tgaagtgatt aaggccgatg cgtatgtgat ggcgtttggt tcttactcga
     7801 cggcgatgct caaaggcatt gttgatattc cggtgtatcc gctgaaaggc tactcgctga
     7861 ctattccaat tgcgcaggaa gatggtgcgc cagtatccac cattcttgat gaaacctaca
     7921 aaatcgccat tacccgtttc gataaccgca ttcgtgttgg cggaatggcg gaaattgttg
     7981 gttttaacac cgagctgttg caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc
     8041 tctatccacg cggcggtcat gtcgagcagg cgactttctg gacgggtttg cgcccgatga
     8101 cgccagacgg tacgccggtt gtcgggcgta cacgctttaa aaatctgtgg ctgaataccg
     8161 gtcacggcac gctcggctgg acgatggctt gcggttccgg tcagttgtta agcgatctgc
     8221 tctctggtcg cacgccagcg atcccatatg aggatctaag cgtagcgcgc tacagccgtg
     8281 gatttacgcc atcacgtccg ggccatttac atggcgcaca cagctaagga aacgagatga
     8341 cccgtccgat acaggccagc ctcgatctgc aggcattaaa acagaatctg tccattgtcc
     8401 gccaggccgc gccgcacgcg cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg
     8461 gtattgagcg tatctggagc gcgctcgggg ccaccgatgg ctttgcatta cttaacctgg
     8521 aagaggcaat aacgttacgt gagcgcggct ggaaggggcc gatcctgatg ctggaaggat
     8581 ttttccatgc tcaggatctg gagatttatg accagcaccg cctgaccacc tgcgtacaca
     8641 gcaactggca gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg gatatttatc
     8701 ttaaagtgaa cagtgggatg aatcggttgg gcttccagcc cgatcgcgtg cttaccgtct
     8761 ggcagcagtt gcgggcaatg gcgaatgttg gcgagatgac cctgatgtcg cattttgccg
     8821 aagcggaaca tcctgatgga atttccagcg cgatggcgcg tattgagcag gcggcggaag
     8881 ggctggagtg tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac ccggaagcgc
     8941 attttgactg ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc ggtcagtggc
     9001 gtgatatcgc caataccgga ttacgtccgg tgatgacgct aagcagtgag attattggtg
     9061 tccagacgct aaaagcgggt gagcgtgtgg gctacggcgg tcgctatact gcgcgcgatg
     9121 aacagcgaat cggcattgtc gccgcagggt acgccgacgg ttatccgcgc cacgcgccta
     9181 ccggtacccc tgttttagtg gacggcgtgc gcaccatgac ggtggggacc gtctcgatgg
     9241 atatgctggc ggtcgattta acgccttgcc cgcaggcggg tattggtacg ccggttgagc
     9301 tgtggggcaa ggagatcaaa attgatgatg tcgccgccgc tgccggaacg gtgggctatg
     9361 agttgatgtg cgcgctggcg ctacgcgtcc cggttgtgac ggtgtaactt gttgtaagcc
     9421 ggatcggagg caacgtcttc tgggtgcaaa aaaaatcatc catccggctg gtcagcaact
     9481 gtagttgtta atgtgacaga gccattgccc atgatagtgt ccattaaaag gatggacact
     9541 atttccccgg aacctgaact caccgcacag gcgttctaca taaaacgctt acgcttcatt
     9601 gttgactcga actcgacttc agataaatca cgctttaccc ttgatggagc ctgtacatag
     9661 atttgtgtaa ttgcctgatt ttgatatgtt caattcaaca tcaaatgaag gttaaattat
     9721 ggacgacaaa caattgcagg ctcaggttgc gttcagcaaa gcatcgcaac cggcgataga
     9781 tgcttcatta aatttaagat tcagcttcct cttcagccac ccgtacgcca atcttcaaca
     9841 cttcattatc ttctttctcg gccaccgtcc agatcatccc ggcaaactct acctggtcac
     9901 cgacaaccgg tgccgcgcct aacaactgct ggacaatttc acccagcgtt tgctgcttat
     9961 cacgatattc tcgcccgtct tccaggccat atatcagcgc aacatcagca tatttggcgc
    10021 tggcttcgag aatgaagtca ccaaagaagc gttgatccag cgcgaccggc ggcgactggc
    10081 tgaacagttt accgagcgcc ggaagatcgc gttcccgacc aattacacac aacacatcgc
    10141 cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg gttatcacga aacagtgccg
    10201 caatacgcgt ctcttttggc atatgcaaat cgcgtagtgc cgcgcccacg caccatttat
    10261 cggcactcaa ttgataaaca aactgctccc acggattttc cggatgaata tccaggccaa
    10321 cgcgtgacac cggacgtccc actggcggaa ccaccacttt ggctttttta gccgcccacg
    10381 agagtgatgt tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg acattaaaga
    10441 acagacgtgc attctccagc cccgccatca tcgggaacac ggccaggatg attggcaccg
    10501 cgccgcgtaa tcctacccag ctgataaaca cgcgctcacg cagattgaaa ccgcggaagg
    10561 ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa tatcatccat gccgacaaaa
    10621 tgagcgccgg aatggcaata ggcagcagat cgcttgggtt aaccaatagc cccagcacca
    10681 ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa attttgcagg atgccgtagc
    10741 ggttgcgaat cggccgatta cccagcagaa aaccgcacag ataaacagcc agaataccgc
    10801 tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat gccagcaatg
    10861 gatataatcc ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa taaccaccgc
    10921 caagtccgat gacaatcccg aggccaaatt gctgcagaat atcgaccacg aacatccaac
    10981 tgacactgct ttcatgttgc tggatcatcg caatcagggt aatcgtcaga aagaccgcca
    11041 ttggatcatt actgccggat tcaatttcca gcgtcgagcc aacacgttcg ttaagcccct
    11101 taccacccag cagagaaaag accgctgcag catcggtaga gccgacgata gcgccgatta
    11161 ataagccttc aatcaaatca agattaaaca gccacgccgc catcatgccg gttaaaccag
    11221 aggtgataag cacgcccagc gtcgccagcg acagtgccgg tcctaacgcc acacgaaagg
    11281 aactggcctg agtgcgcatc ccgccgtcga gcaaaataat cgccagtgcc aggttactta
    11341 ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg actcctgcca
    11401 gcatgccgat cgccagaaaa ataaccagaa taggaatgcc aagacgggaa gaaaatgaac
    11461 taagtaatat actgctggtg acaaggatgg atcctaagat gaaaaggcta attattgttg
    11521 tggcatccaa cggtcggtta ctcctgatta cgctgtctct tatataaacc ctaccatatt
    11581 agcggcagag acagcgtttt acttagccct gaagcgtgat ttttttacat tttaagaaca
    11641 ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt tattcagaat ggcatgtgcg
    11701 cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt aatgagtgga
    11761 atcgacaggc gggaacgcaa aaacgcgtac actgactcca ggttgtaacc tgcgtcataa
    11821 tcattgggcg tgctgccgct aaagctaccg agaataatcg ccttctgacg tggcaaaatt
    11881 cccgcatgat agagctgcaa cagcatacgt tcgacgcgga aagggtgctc gttaatatct
    11941 tccagcacca gaataccgtt ctcaattttt ggcatccacg gtgtaccaat cagtgaaatc
    12001 agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg gccttcccct
    12061 tgccattcaa tggtgaaggt ttcattgcgt aacgccagcc agaaatggtg ctcggtaaag
    12121 gcgttcagtt catccgcgcc aaagtttgct accagcatgg ggccgctaaa ggtgatgaca
    12181 ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat cgctatgccc gcatatgagc
    12241 aacgggtcat attgttggcg agcaaccagt gcctgccagt caatatctgc cagtaaacga
    12301 ctggcaccgt aaccgccgcg tacagccagc acgatggtgt tgggggtggt cagtctggca
    12361 agggaattga gatcttccag gcgctccgtt tccgtacccg caaaacgctc acaacgacgg
    12421 gcaataacct cgacgttatt gacctgatgc ccctcgtcgg taaggcgctg gataccacgc
    12481 agcgcggcgt gctgtttaat gcagtaaccc gatggggcaa ttaagtgaaa cagagacatg
    12541 gcaattcctt gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt
    12601 cacctcaacg gcgattccag gctataagga tagaagaagt gaaattgaga tggtttgcct
    12661 ttttgattgt gttattagcg ggttgttcat caaagcatga ctatacgaac ccgccgtgga
    12721 acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc aataagccag aaagccggtg
    12781 cagcctgggg cgtcgatcca caattgatca cggcgattat cgctatcgaa tcgggtggta
    12841 atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct
    12901 ccggacgtga tgtttatcgc cgtatgggct ggagtggtga gccgacgacc agcgagctga
    12961 aaaatccaga gcgtaatatt tcaatggggg cggcttacct gaatattctg gaaaccggcc
    13021 cgctggcagg cattgaagat ccgaaggtac tgcaatatgc gctggtggtg tcatacgcta
    13081 acggggcagg tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa
    13141 tcaacgattt ggatgctgac gagttcctcg atcacgtagc gcgaaatcat cctgcgccgc
    13201 aggctccgcg ctatatctac aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg
    13261 cactttgtcc gctttttctc gggcttctcg ctcgagagag aaaataatcc gctgtaattg
    13321 ccgctccacc gtcgggctga cgttaagaaa acggaagctc agacggggag tggtgatggt
    13381 ttcattcttg ccatcaatca ctttgcgctc gctgatggag attaactggg cgtcaaagtg
    13441 aaaaacaccc cattgcccca tgttgacttc aatctgagcg aagcgcatgc cttcttgtaa
    13501 ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg cctaacgaca aatcatacag
    13561 gcggaaacgt aacgtactgt tatccgccag tttggtctgg caaaaataag gcggatggag
    13621 tggggcggag atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac
    13681 ggtgataaat gccggaagct gcaagtattc actctgctgt agttgttcaa cagtaaactc
    13741 gactttcgca ccctgagttt cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat
    13801 gttgtcttcg gcttgactgc cgaaatccag caccagttta tccggggtta ttgccagtat
    13861 tttgctgatc agctgcccgc cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa
    13921 atcgcgtaac acgcccagga cggctaacgg attttgtttc aggaactgct catggtaatg
    13981 actcacgcgg aaaaactcct gactgacaaa ctgtctttgg tttatcggtc acagttaaca
    14041 aaacttaata caaatgcgtg aatatttttt acatgttgtt cttaaatcag ccgacatacg
    14101 cccagcatcg atcccgccct tgcctatact tagagcgttg acgtaagcat ttcttgcgtc
    14161 cgattcatcg aaacgagggc ctgaacatgg gaattattgc ctggattatt tttggcctga
    14221 tagccggcat tatcgccaag ctaatcatgc cggggcgtga tggtggtgga tttttcctga
    14281 cctgtattct cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc atgtttggca
    14341 ttggcggctc catcagtggt tttaatctgc acagcttcct ggtggcggtg gtgggagcta
    14401 ttctcgttct gggcatattc cgcctcctgc gaagagaata agattttcat aaggcggata
    14461 gcgatacaga tgccgctatc cgctttcaca tcagaacgta tactcgacac ctgctttacg
    14521 ggtgaaaaaa atcaatatag cacttcgata tccgataaag tcggttcccg gtaacaacaa
    14581 cagaatcctt ttttaattat tgtttcgttg tttttggtgt gattcagaaa ataaatattt
    14641 tagacagtag ccagtcaggc gttaaaaatg gggcggatag tgaggcggtt ttggctgcgc
    14701 ttattgaaca tttagaattg atcaatcctt ctggcagatt aagccaaaat acccgttcgg
    14761 caatgcttca gttacgcgaa gaggaatggt ctgaattctt tttttggttg ctaaattctc
    14821 tggagtgtct ggattatgtg attattaatt taactccaga aagcaaaaaa acgttgatga
    14881 gtgaacaccg taacaatatt caggtagcaa ttgatgcttt atatagccaa cgtcggcgta
    14941 aatcgccggg tgatgaaagt gaaacactta cccgtagaaa tgacgctatc ttcggcaacc
    15001 acgtatggca aacatttgcg caatacttcc cgccaggact tgaaaaaccg tctgtgtaga
    15061 aaaaaccata gctatggtgt tatctgccga caacgccatc cggagaagcc gaatgacgtt
    15121 gtcggagtaa gcgctggtta aggtgtgggt tgtgcctctt tggttgaggg ttgcgtcgtt
    15181 gctgacttaa cggtcggacg cgtcgccgga acattgtcac acggttgctc tttcgggcag
    15241 atcaaatcca gcattttcag cgtcacgcca ttggtccagc caaagccatc ctgtaatgga
    15301 tattcgccac cgccgccccc cgttccggtg gtgctgacat catatttttc caccagctta
    15361 ttctcccggt cataggtgtg ctgaacattg gtcaggaagt gccagctaat gtccatcgcc
    15421 acctcttttt gcccgtagtt ttgtaatcct tctgtcgcga cccactgtaa cggtgcccag
    15481 ccatttggcg catcccattg ttgcccactt ttcaccgacg tggtgttcag gccgccgggt
    15541 tgcagcagat gtgttttcgt cgccgtcgcc attttgttgg cgcgatcttt cgctgccgca
    15601 ttgacgtaca gcgggaacag ggcggccgcg gttaactgat tgcgcacttt atgacttttc
    15661 aggtcgtaat cggcatacca gccttgttga tcgttccaca tgtatttttc gatccctttt
    15721 tgacgggcat ttgccagcgt ttcgtactgg tttgccatcg cgttatctcc ggcagctttg
    15781 ctggcgcggg cgaggatttt ttccatttta aacatcaggc tgttcagatc gaccggtacg
    15841 atgctggtgg tgcgtaaggt atttaactgc tgcgggttgt ccatccagcg cgagctgaaa
    15901 tcccagccag acgcagcggc agagcgcagg tcgcggtaaa tttcagtggc aggtcgattc
    15961 ggattgcttt tggcggtggc aatatcttcc acccatgact ctggtcgtgg cgtatcgcga
    16021 tcgtcccagt agcggttgag aagggtacca tcctgaagtt ttacaacgcg tttttcctgt
    16081 tgtccggctt gcaggttttc aacaccgtcc atccagtaag catattcttt ttgcatttgc
    16141 ggcaggtatt gcttcaacgc ggcatcgcct tcatgctgcg ccagtaactc taccatcagg
    16201 gcaaagaagg gcggttgcga gcggcttaaa tagtaactgc ggttgccgtt gggaatatga
    16261 ccgtaagtgt ctatttcatg agcaaaattg gccaccatat ccgcgacttt atcccagtga
    16321 ccgctttcgg caagtcctaa catggtgaag taactgtccc agtaatatac ctcgcgaaaa
    16381 cgtccgcccg gcacgacata aggttccggc agcggtaaca gagaatccca tttttcggtg
    16441 ttttcggtag aacgcgttaa taccggccaa agtccgtcaa tatgttcgcg cagtgactgc
    16501 ccctctggcg gaacatattt ctcgccttct ttcggcaggg tgaaattgac gttaacgaaa
    16561 tggcgcagat caaatccgct ctggttttgc tgcatccgat aatcagcaag gatcatcagc
    16621 ggatcgctgt tcggcacggc atcggcaaag gttttttggt ccggaaaaag tttggcgttt
    16681 tgcacatcat taaacagcgg ccctaataaa atatcaggcg gctgtggtgt taccggtgtt
    16741 tcttttgcct gcaccgatag cgcagcgaaa cacaaaaaga tacaggctgg aattaacgcc
    16801 attttttgcg ggcgagaagg tgcgggggat ttcatcaatc attctccttt ggcgaaaccg
    16861 aataaaacgg tattcaagta tcagaaaacc ttagttcagg atcgcactag ctgcatgatc
    16921 gaaatctcat tctgcgaact gtcaggcatt ttagaaaagg gaattaaagg gataaataac
    16981 ggcgaaatag agtgatcaaa taacgtctgt ttattagcca gccagcggcg ttgggttcgt
    17041 gattagcgaa gaaaaatcat cataaattgg tgaaaaatat aacaggtagt taaaaaccat
    17101 tagtgctgag taaattgccg gatgacatca gaacgatgcc atccgaacag tggcttaacc
    17161 ctgacggttg aaacgttgcg ttttaacgtc cagcgttagc gtttcttctg gttgtatcgc
    17221 atacagtttc tcaccctgct ggcaaatcca gccaatcccc agttcacggg cgattagggc
    17281 gctgtgggat accggacttc cggcgctaag gcagatacct tttacaaccg ccggatccag
    17341 ttgcagtact gtggaaggat aaatgttctc cgccagtaga atagttggcg agttaaactg
    17401 cgggagttct tctttcgttt gggtcaggtg gaccagggtg cgatgcagaa gatcgtccac
    17461 atcaatatag cgagcttgta gatattcatc atccagttgc tggtattgct ggctaagttc
    17521 tttaagaact tgctgccagg catattctgc cgtgcaatgt tcatgctgaa ggagttcgct
    17581 tgccgccgcc agcagttccg gatcatctaa cagtgtatgg tgaccagaaa agattgcggc
    17641 aatatcgtca agcccgctgg cttctgcttt cgctgttaac gtcatcagat ctaataacgt
    17701 gaagtcaata gcctggcgta atcgatcttg ttcttcttcc acggtcaggg ttgattttgc
    17761 ctgtaccgta cataaaactg gttgataata aaaggcttta cccgaaacag gcggaacggg
    17821 acgcagagta ggtggagcga cttcctccgt ttcaccaaag ttatcttcag ccagctgacg
    17881 gaaagcgatc agtgcctctt cagcttctgg ccctttcgca atcaggcgca gcgtatcgtt
    17941 atagcgaact tgtagtaacg caatctggtt aatactctct ggtgtgacgc atttgccgtt
    18001 tttttccagc aacatatcgg cattaaatgt cgataaggta taaaccagcc gggaggccgg
    18061 acgtacatgc aggccgttac ggttttttat gaccaccgcc agagaacggg cttcttcatc
    18121 gtacgcagga catgtgtcag agatttcagt gtcggaggac ggtaaaccca gttgttcacg
    18181 tttggcttcc agcgcatgca tagcgtcaaa gataactttg tcgatatccg ctcccgaggc
    18241 cgcgctgacc gttgctgcca gtgtaccttc gaccaacggc gcagcacaca aacgtacttt
    18301 tgcggcgatc ttgggagcca gcaattccag cgcagtttca gcactcaata atgcgctacc
    18361 catatccatc atgaccagca catggtcggc atcagcaaca gattcgatgg cctccatcac
    18421 tttgacggca tcggtaccaa taggattttg tggatcgtca attcccgcgg caatggcgat
    18481 tttacaacta tcactcatta acatctgacg ggctaattca ccgacacctt ctcccagtcg
    18541 gctgctatgt gaaactatga ccaggtttac catcaccaat tccttactct tttgcggcta
    18601 acgccaacat ttgcatcata aacatcaccg aggtcgcgcc gggatcctgg tgaccaatac
    18661 tgcgttcacc gagataactg gcgcggcctt tgcgggcttg catcgtaatc gtactttgtg
    18721 cagcggattc ggcgatgcta ctggcagctt cgagcgccac cggaacagag agattttgct
    18781 cgctggactg acgtaacgat tccaccacag gcacccacac atcactcatg gttttatcgc
    18841 caggttcggc tttcccgcga ctgattacgc cgtccgcgcc atcgcggaac atctgataaa
    18901 gctcttccag tgtcaggctt tgccgtgcct gggtcgcctg tgcggcgcgg ataaagaagg
    18961 taccgaacag cggaccactg gcaccaccga cgctggaaag cagcgtcata ccggtattct
    19021 tgagaatgaa accgatatct ttatctgcga tagcagggag tttttccacc actttgctaa
    19081 agcctcggtt catatttagc ccgtggtcag cgtcgccaat ttcgcgatcc agtccggtaa
    19141 gatactcgct ctcggtgctg aaaatatcgc cacaacgagt gagccagtta acaatttgag
    19201 ttctgctcag tgacattgct ttctccttat ttaccccagt taagggccgg ggtgtggacc
    19261 ggggcgtccc agagtgccag cgtttcgtca tcaactttca gtaaggtgat tgagaaaccg
    19321 gtcatatcca gtgaggtgca gtacgcgcca attaaattac gttcgatagt caatcccgct
    19381 tgctggcaac gtgtggtcag gcggttatag acgccgtaca gctcagaaag cggagttgcg
    19441 ccaagattgt taaccagcgc aatcacccga tcgccagact ggagcggttg tttggtttgt
    19501 tgttcttcct gccaactgcc ttgttgataa tcccagaaac gcaaagtgcg atggtatgag
    19561 ccattttcca gcagggtgtc gaacatttca tcgacggttt gatcaaggga agagaaggag
    19621 cggcggtcaa tacccggctc accatgaatg ccgacgccaa actccatctc attatccgcc
    19681 agggtaaaag aaggtttgcc cgcggcagga acggtacagg caccgagagc gatacctatt
    19741 gagtggcctt gattattcaa cttacgcccc agttccgcac aggcgtccag tgagtcgcca
    19801 cgctccgccg ctgcgcctac gagtttttca attaatacgg tgttggcaac gccgcgtcgc
    19861 ccggcagtat aaagactgtc ttttaccgca acgtcgtcat caatgaccac agtggtcact
    19921 tttacgccgc tatcgtgcag taactcggtc gctgtttcaa agttaagaat atcgccggtg
    19981 taatttttga taatcaacag tacaccttcg ccgccatcaa tttgcatggc gcattcaaag
    20041 attttatcgg gcgtcggtga ggtgaaaatt tcgcccggac aggcccccga aagcatcccc
    20101 tgaccgatat aaccacagtg catcggctcg tgtccgctgc cgccacccga cagcagggcg
    20161 acttttcctg caacgggggc atcagctcgg gtgacataca ccggatcctg atgcagtgtc
    20221 agcgatggat gcgctttcgc cagtcctgcc agttgttcgt ccagtacgtc tcgcacatca
    20281 ttgatcaatt ttttcattat tttgctccag caattacggt agggcatgga tgatgttcaa
    20341 cgacacggcg accactgact gccgatgaat ccattgtgca tcaggcaagg gaaaagaaaa
    20401 ttagcccaaa ttatgtttca tagtgaaaca tatgctttaa tgaatgttcc atattgaaac
    20461 ttttacgtgt attaatactt aaaattgcga gccggaacac cttttgtcat aagggatgcg
    20521 ggatatgagt ggcgctttta acaacgatgg tcggggcata tctcccttaa ttgcaacctc
    20581 ctgggagcga tgcaataagc tgatgaaacg ggagacatgg agcgtaccac atcaggccca
    20641 gggcgtgaca tttgcttcta tttatcggcg taagaaagcg atgctgacgc tcgggcaggc
    20701 tgcgctggaa gatgcctggg aatatatggc accgcgagag tgtgcgctgc ttatcctcga
    20761 tgaaaccgcc tgcattctca gccgtaatgg cgatccgcaa accttgcagc agctaagtgc
    20821 actgggattc aatgacggca cgtattgcgc tgagggaatt attggtactt gtgcgctatc
    20881 gttagcggct atctctggtc aggccgtgaa aacgatggcc gatcaacatt tcaaacaggc
    20941 actctggaac tgggcctttt gtgcaacgcc gttgtttgac agcaagggcc gattgacggg
    21001 aacaatagcg ctggcgtgtc cggttgagca aactaccgca gctgatttgc cgttgacgtt
    21061 ggcaatcgcc cgcgaggtcg gaaatttact gctgacggac agtttgctcg ctgaaactaa
    21121 ccgtcattta aatcaactta atgccctgtt agaaagtatg gatgatggcg tgattagctg
    21181 ggacgagcag ggtaatttgc aatttattaa tgcccaggcg gcgcgggtct tgcgccttga
    21241 cgcgacggca agtcagggac gggcaatcac tgaactctta acgttacccg ccgtattgca
    21301 acaagcaata aaacaggcac atccgctcaa acacgtagaa gcaacctttg aaagtcagca
    21361 tcagtttatt gatgcggtga taacccttaa accgataata gaaacgcagg gaaccagctt
    21421 tattttgttg ctccatcctg tggaacagat gcggcagttg atgaccagtc aattaggaaa
    21481 agtcagccat accttcgctc atatgccaca ggacgatccg caaacccgcc gcttgattca
    21541 ttttggtcgc caggcggcgc gcagtagctt tcctgtcctg ctttgtggag aagagggcgt
    21601 gggcaaggca ctgctaagtc aggcaattca taatgaaagc gagcgtgctg caggtcctta
    21661 tatcgccgtc aattgtgagt tatatggtga tgcggcactg gcggaagaat ttattggtgg
    21721 cgatcgcacg gacaatgaaa atggccgtct gagtcggctg gaactggcgc acggcggcac
    21781 gctgtttctt gaaaagattg aatatctggc ggtggagtta cagtctgctt tgcttcaggt
    21841 tatcaagcag ggggttatca cgcgactgga tgcgcggcgt ttaataccaa ttgatgtcaa
    21901 agtgattgca acaacgaccg cggacctcgc aatgctggtg gaacaaaatc gttttagtcg
    21961 ccagctgtat tacgcgctgc atgcatttga aattaccatc ccgcctctgc gtatgcggcg
    22021 tggcagcatt ccggcgttgg tgaataacaa attactcagt cttgaaaaac gcttctctac
    22081 gcgtctgaaa attgatgatg atgccctcgc tcgcctggtt tcttgtgcat ggcctggcaa
    22141 cgattttgaa ctttacagcg tcatcgagaa tcttgctctg agtagtgata atgggcgcat
    22201 tcgcgtcagt aatttgccgg aacatctgtt taccgagcag gcaacagatg atgtcagcgc
    22261 cacacgcctt tccaccagtc tgtcatttgc ggaagttgaa aaagaggcaa ttattaacgc
    22321 agcccaggtc acaggcggtc gcattcagga aatgtcggct ttacttggaa tcggccgcac
    22381 tacgctgtgg cggaaaatga agcaacatgg cattgatgca gggcagttta agcgccgggt
    22441 atgaaagaca gaaacgattt ctgatacatc agagtgatct atatttcatt ccggcgcacg
    22501 ctaacatttt tcagcatcgt ttaaggactt gtctatcccg cacttaaaag ctgaagcgat
    22561 atcctccgtt gacttgcttc tgatcaaaga ggttaccctg cgtgtaatcc gcttcgaggt
    22621 agaatgtgtg ctgtttgtta tactgtgcac tgactcccac gccattattc cagccattac
    22681 ctttgaaact gtacttctcc cgggagttgt tcaacagata ttctgtatcc cctgaaaact
    22741 cgcggatagc gccagtcttc acatagatat tcagctggct gttgcctgcg gtgatgtcat
    22801 atcccagtat catgctggca cgccccagca gcgattcgta gtgattcaga tgtatattga
    22861 ggccattact cgccttcata gccatctcat tctggtggct gtatgtaagc tgggtttacg
    22921 gctctatata gaacccataa ccagtagggg acaggttgaa cctctgcccg gcttccaggg
    22981 agatgctcat tccattcgca gtgccgttgg cgttaacgcc gttgttctga ctgtccagta
    23041 cgtggaaact atttttctgg cgcgatgctt ttataacgag atcgctgtaa aaaccgtttt
    23101 gtgccatgta actggcgtac attcccatgt agtctgaacg tgcggtaccg tcgcctccgc
    23161 tatagtccgg cgatgcatgt gttgagccaa tatacagacc gacatacaac ggcattacat
    23221 cagagagacg tttatcccca ccaaactgga taccgctgta acccatgtca aagccgctca
    23281 gtttgccact ggcaaaggag tccaggcttc ccccataact gcgcaaccag atattaccgt
    23341 ctttactctg atttcgcagg tcacccatcc gttgcatcag cgtacggttt tcaacatagt
    23401 tcaataagta accgacattg agataattgc cgccagcatc tgcggtcgtg gtgggtttag
    23461 gcgtgggagc gggttcaggc gtaggatcgg ggttgactat gggaggctga gcgggagctg
    23521 gtgtgggttc aggattagga gtcggttccg gaactgtccc ggaagcgtaa agctcccagt
    23581 tagtgccatt tttacgcaca tcgtacagat atccccccaa ctcaacctga gaagacgcgc
    23641 tgaacgaggc cgcgccgtca gtggttttta ccactgtcag aacttcattt cccgttgtgg
    23701 cctcgctgcc ctggttgcgg atagccaata cgtgattacc agcactactc ccgctgatat
    23761 tcaataaatc ccctttatta ttaacgccat taccctcgcc aacaacatca gcacgcataa
    23821 taaaggtact gttaccgctc aggttctcta cgtttaatgt ggcaaatgtg ccggcagttg
    23881 acccgtggct ggcaaaatcg acagttgaat ggctcagcgc cagcgtgtcg agattagaat
    23941 tacttgttac gttccagacg ctgttattca ttgcaacgtc cagttttccg ccattgacat
    24001 tatcgctgag ggaggaacct gtccaaaccg acccagggtg catatccaga ttgattagcc
    24061 cacctttgga aagaacgcta ccgttgataa gcatacgacc cgaggcgttg atgcggctgg
    24121 cggcataacc atcgtcatgt tgcgttgcga tggccatctg gtcgggtgta ctcatatcaa
    24181 tgaccagatc gctcgtgagg tcgatctggc tgttggtcat ggcataaatt cccctggctc
    24241 ctgccgcgcc ggtgatagcc aaactgtctc cggttattct accgccgctg agcgcccaca
    24301 aacccagcgc cagactgcca ttacgatcaa ctgtaatatc ggtattttgc atgttgacaa
    24361 cagccgttgc cgtctgggcc gaggcactat aagaaccgcc ggaaaagatg ctgtttcgtt
    24421 gcgctgccgt gccagtaaaa ttgattatcg cgtctgaacc actggtgacg aggccaccgc
    24481 cctgcgcgga agaaatatgg ctatctgcac cgatagtggt tgtaccacca cgaacttcta
    24541 cgccattggc cgcggctcca gttacatcaa cagtgagtgc attcgcgcta acctggccaa
    24601 agctccagag gccgtgagca ttatcgccat tggttttaat ggtactgttt gttccgagat
    24661 cgacaacaga gtttttctgt acgtttatcc ccatggcgct gtagccctga acatcgattg
    24721 tcaggtctgt agccgtaaaa cgcgcagcac cattggcgtt attgccgttg agaccaccga
    24781 tataaacacc tgtacttcca tcggtcgtga ttttacttcc gcttccaaga tcgacactgg
    24841 tgccatagtc attgatggtt aggcctatac cgttcgagtt ttcaatggtg aattgagtgg
    24901 ctgttagtgt tgagctatgc ccaataatga tgccgtcatc gttactctta atggtgctgc
    24961 ctgtgcctaa gtcagcatgg gtatagtcac caattaagtt aatgccgatg gcagaggttt
    25021 gaccaacaac atctactgtg agtcggttag ctgtcaggga gctgtttttg ccctgaataa
    25081 tgattccttt tgcatatcca gaggcgtcgt tgacattgac ggtgacatca ttacccaggt
    25141 tgatattgcc aggcgtgttt cctgctgggg tcattacacc atagaggttt cctgatggat
    25201 cggcggtatc tcctgtaatt ctgtcaccat cattaatagt aatattggtg ccatattgac
    25261 ttcctgtatc agcagctatc agcagggggg aaaagataat tgcattcaaa ccaatagcgc
    25321 caaattttat cagttgaatt gagggcgaac ggatttttaa ttcagcgaga cgatctgctt
    25381 tggtattccc attgtgttgt ttgatgccca taccactcct atatagtacc cagactatgg
    25441 gtctatggat tttgtcattt tgtttaaatc aatgaataat atcctcttat catcatgata
    25501 tttgtatggt gcaatcatg
//
LOCUS       DAJSKJ010000042        25393 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000042 DAJSKJ010000000
VERSION     DAJSKJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.042"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(162..542)
                     /locus_tag="ON345_001480"
     CDS             complement(162..542)
                     /locus_tag="ON345_001480"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR3071564.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(542..1273)
                     /gene="pdxJ"
                     /locus_tag="ON345_001481"
     CDS             complement(542..1273)
                     /gene="pdxJ"
                     /locus_tag="ON345_001481"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR3071565.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1285..2013)
                     /gene="recO"
                     /locus_tag="ON345_001482"
     CDS             complement(1285..2013)
                     /gene="recO"
                     /locus_tag="ON345_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR3071566.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDANTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2025..2930)
                     /gene="era"
                     /locus_tag="ON345_001483"
     CDS             complement(2025..2930)
                     /gene="era"
                     /locus_tag="ON345_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR3071567.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(2927..3607)
                     /gene="rnc"
                     /locus_tag="ON345_001484"
     CDS             complement(2927..3607)
                     /gene="rnc"
                     /locus_tag="ON345_001484"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR3071568.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(3879..4853)
                     /gene="lepB"
                     /locus_tag="ON345_001485"
     CDS             complement(3879..4853)
                     /gene="lepB"
                     /locus_tag="ON345_001485"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3071569.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(4869..6668)
                     /gene="lepA"
                     /locus_tag="ON345_001486"
     CDS             complement(4869..6668)
                     /gene="lepA"
                     /locus_tag="ON345_001486"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR3071570.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(6866..7345)
                     /gene="rseC"
                     /locus_tag="ON345_001487"
     CDS             complement(6866..7345)
                     /gene="rseC"
                     /locus_tag="ON345_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR3071571.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7342..8298)
                     /gene="rseB"
                     /locus_tag="ON345_001488"
     CDS             complement(7342..8298)
                     /gene="rseB"
                     /locus_tag="ON345_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR3071572.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8298..8948)
                     /gene="rseA"
                     /locus_tag="ON345_001489"
     CDS             complement(8298..8948)
                     /gene="rseA"
                     /locus_tag="ON345_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR3071573.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(8981..9556)
                     /gene="rpoE"
                     /locus_tag="ON345_001490"
     CDS             complement(8981..9556)
                     /gene="rpoE"
                     /locus_tag="ON345_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR3071574.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9553..9708)
                     /gene="rseD"
                     /locus_tag="ON345_001491"
     CDS             complement(9553..9708)
                     /gene="rseD"
                     /locus_tag="ON345_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR3071575.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWCFDSAWKFG
                     LGRLYLG"
     gene            9964..11586
                     /gene="nadB"
                     /locus_tag="ON345_001492"
     CDS             9964..11586
                     /gene="nadB"
                     /locus_tag="ON345_001492"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR3071576.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVIGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11571..12308)
                     /gene="trmN"
                     /locus_tag="ON345_001493"
     CDS             complement(11571..12308)
                     /gene="trmN"
                     /locus_tag="ON345_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR3071577.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVCFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12440..13774
                     /gene="srmB"
                     /locus_tag="ON345_001494"
     CDS             12440..13774
                     /gene="srmB"
                     /locus_tag="ON345_001494"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR3071578.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(13807..14688)
                     /locus_tag="ON345_001495"
     CDS             complement(13807..14688)
                     /locus_tag="ON345_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3071579.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQGFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGEMLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            14791..15378
                     /gene="eamB"
                     /locus_tag="ON345_001496"
     CDS             14791..15378
                     /gene="eamB"
                     /locus_tag="ON345_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR3071580.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15434..15817)
                     /gene="grcA"
                     /locus_tag="ON345_001497"
     CDS             complement(15434..15817)
                     /gene="grcA"
                     /locus_tag="ON345_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR3071581.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16122..16811
                     /gene="ung"
                     /locus_tag="ON345_001498"
     CDS             16122..16811
                     /gene="ung"
                     /locus_tag="ON345_001498"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3071582.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(16859..17896)
                     /locus_tag="ON345_001499"
     CDS             complement(16859..17896)
                     /locus_tag="ON345_001499"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR3071583.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRTEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18103..18522
                     /gene="trxC"
                     /locus_tag="ON345_001500"
     CDS             18103..18522
                     /gene="trxC"
                     /locus_tag="ON345_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR3071584.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18591..19289
                     /gene="tapT"
                     /locus_tag="ON345_001501"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18591..19289
                     /gene="tapT"
                     /locus_tag="ON345_001501"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR3071585.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19321..21981
                     /gene="pat"
                     /locus_tag="ON345_001502"
     CDS             19321..21981
                     /gene="pat"
                     /locus_tag="ON345_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR3071586.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSTAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGIMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22095..23450
                     /gene="pssA"
                     /locus_tag="ON345_001503"
     CDS             22095..23450
                     /gene="pssA"
                     /locus_tag="ON345_001503"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR3071587.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGIDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23496..23819
                     /locus_tag="ON345_001504"
     CDS             23496..23819
                     /locus_tag="ON345_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR3071588.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(23816..25114)
                     /gene="kgtP"
                     /locus_tag="ON345_001505"
     CDS             complement(23816..25114)
                     /gene="kgtP"
                     /locus_tag="ON345_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR3071589.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMILGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT         6103 a         6726 c         6361 g         6203 t
ORIGIN      
        1 tttttatgct aaagattgca agttgcttgt aaaagataag aacactgatc cataatcgct
       61 gttgttgagg gtgcatgctg cacaaaatta aagttaaaaa ataaaacccc cgttccttac
      121 cagttcgggg gttttacttt ttaaagagaa cggtattatt tttaactttc aataattacc
      181 gtggcacaag catagtgccg ctcatctgcc agcgttacat gcatatttac aacgccgagc
      241 ttttccgcca gttttaatgc ctcaccccat agccgtagcc gtggtttgcc gagctcatca
      301 ttgaatactt caaattgatt aaacgccaga ccattgcgga tcccggtgcc aaacgctttt
      361 gctgcggctt ctttcacagc aaaacgcttc gccagaaaac gcaccggctg gtggtgcgtt
      421 ttccagattg cccattcgtt atcgcttaat acgcggcgtg ccaggcgctc accggatcgg
      481 gcgatcactg cttctatgcg agcgatctct acgatatccg tgcctaaacc taatattgcc
      541 attagccacg cgcttccagc atcagacgct tcatttctgc caccgcatct ttcagtccgg
      601 tcatcactgc acgaccaata atggcatgac cgatattcag ttcatgcatc tcagggatgg
      661 cggcaatagc tttcacgttg tgataggtca gaccgtgacc agcgttaact ttcagaccga
      721 gactagcggc aaaggtcgcg gctttggcga tacgcgccag ctcttgcgcc tgttcggcgt
      781 cagttttggc atcagcatag caaccggtgt ggatctcgat aaacggtgcg ccaacctctg
      841 ccgcagcttt gatctgctct tcatcggcgt cgataaagag agaaacctgg atcccggcat
      901 ctgccagacg tttgcaggca tcgcgcattt tgtcacgctg ccctgcgacg tccaggccgc
      961 cttcggttgt tacttcctga cgcttttccg gtaccagaca gcaaaaatgt ggcttcgtct
     1021 caacggcgat cgccagcatc tcttcggtca ctgccatctc cagattcatt cgggtatcca
     1081 gcgtctgccg caggatgcgc acatcgcggt cagtaatgtg gcggcgatct tcacgtaaat
     1141 gcacggtaat gccgtccgct cccgcctgct cggcaataaa cgccgcctgc accggatccg
     1201 ggtaagcagt accgcgtgcg ttacgcagcg ttgcaatatg gtcaatgttg acgcctaaca
     1261 gtaattcagc catgacaatc ctcatcattc ataatgtgtt ttcaccgttc gcttaggcat
     1321 aaactgccgg aacagttccc tgctctttaa tggtttaccg ccaagatacg gcttaagcgc
     1381 catgcgggta aagcgtttcg cggcgcgtag tgtgtttgcg tcaggaaatt cccgcgcgtt
     1441 taacgctttt aactgccttc cggtgaacgt tttattgtcg ataacgacgc ttgcgataaa
     1501 ccctttttct tcgcgataac gatacgtcat ggtgtcatct accggctcgc cgctacccgc
     1561 gcaatgggta aaattgacgc catagcccag atgcccgagc agtgccagtt caaagcggcg
     1621 cagcgcgggt tctggcgtac cagtggcacc tgcaagagac tgaatgcagt gcaagtaatc
     1681 gaaaaagagt tcagagaagc gcgtctcgta ttccagtacg cgggagagaa gttcgttgat
     1741 gtacagaccg ctgtaaagcg tgataccgct taatggcagc gccagcgaga cagcttcagc
     1801 actgcgcagc gttttgactt cgccacgccc gccgaaacgt agcaagagag gggtgaaagg
     1861 ctgtaatgca cctttcagag tagagcgttt agagcgtgcg cctttggcaa ccagacgcac
     1921 gcgacccgat tcctccgtga agacgtccag catcaggctg gtttcgctcc acgggcgact
     1981 atgcaggaca aatgcgcgct gccagccttc catcggagtg actcttaaag atcgtcaacg
     2041 taaccgagac tgcgcagtgc gcgttcgtcg tcggcccaac cggatttcac ttttacccac
     2101 agttcaaggt ggacaggcgc ttcgaacatt tcctgcatgt ctttacgcgc ttcaatcccg
     2161 atggttttga tcttggcccc tttgttgcca ataaccatct tcttctgccc ttcacgctca
     2221 acgagaatca aaccattgat gtcataacca ccgcgttcgt tagagacgaa acgttcgatc
     2281 tccacggtca cggagtacgg cagttcagcg ccgaggaaac gcatcagttt ttcgcggatg
     2341 atttcagacg ccataaaacg ctgtgagcga tcggtgatgt aatcttccgg gaagtgatgg
     2401 gtcgcttcag gcagatgctt acgcacgatt gctgcgatag tgtcgacatt cagcccggtt
     2461 tcagcagaga tcggcacgat atcgaggaag ttcatctgac ttgccaggaa ctgtaagtgc
     2521 ggcagcagat cggctttttc ctgcacgtta tccactttgt tcaccgcgag gatcaccggc
     2581 gctttgccat cgcgcagttt gttgagcacc atttcgtcgt ccggcgtcca gcgggtgcct
     2641 tcaacgacaa aaatcaccag ctcaacgtcg ccaatagagc tgctcgccgc tttgttcatc
     2701 aggcggttaa tagcgcgttt ttcttccata tgcaggccag gggtatcaac gtagatcgcc
     2761 tgatacgcgc cttcagtatg gatccccaca atgcggtgac gagttgtttg cgctttgcgg
     2821 gaggtgatgg agatcttctg ccccagcagt ttgttcaaca atgtggattt gccaacgttc
     2881 ggacgtccga cgatggcaat aaatccgcag taacttttat cgatgctcat tccagctcca
     2941 gttttttcaa cgcctgttcg gcggcagcct gctcagcctt acgacggctt gaacctgtgc
     3001 caaccaccgg ttcactcagg ccgctgacct ggcagtggat agtaaattcc tgatcgtgcg
     3061 cttcgccacg tacctggact accagataag tcggcagcgg cagatggcga ccctgcaaat
     3121 attcttgcaa gcgcgttttc ggatcttttt gtttatcgcc tgggctaatt tcgtccaaac
     3181 gagtttgata ccagttgagg attaatttct cgacggtttg aatatcactg tcgaggaata
     3241 cgccaccaat taatgcttcg acggtatcgg cgagaattga ctcacgacga aatccaccgc
     3301 ttttaagttc acctggccct aaacgtaagc actcgcctaa ctcaaattcg cgcgccagtt
     3361 ccgccagcgt attgccacgg accagcgtgg cgcgcatccg gctcatatcg ccttcatcca
     3421 cacgagggaa acggtgataa agcgcattgg cgataacgta gctcagaata gagtcgccta
     3481 aaaattctaa acgctcgtta tgtttgctgc tggcactacg atgagttaat gcctgctgca
     3541 acagttcctg atgattaaaa gtgtagccca gcttccgttg aagccgatta attacgatgg
     3601 ggttcatgcg ataccaataa ataaatgcgt caacaattca gcacacgaaa cagacctgat
     3661 atacatggct ctgctaactg cttcgctgca gtttcagttt agcggtatat ggggaccaac
     3721 gctgtttcgt gtgccgtggc aacctggagg tgccaacctt aaacttcggg ggaatattct
     3781 atacacaacg acgggggatg tcgttagcca cgggagattt atctcataaa taattcacgt
     3841 tgtcgccata acggcgacaa cgtgaacgaa gatggctatt aatggatgcc gccaatgcga
     3901 cttaagcgca caccagtcgg ccattcgcct tcttgcttat cgaagctcat ccagatagcc
     3961 gtggcccgac cgaccagatt cgcttcaggc acaaagcccc agtaacggct gtccgcgctg
     4021 ttgtcgcggt tgtcgcccat catgaagtat tgtcccggcg gaacaatcca ggttgccagt
     4081 tgttgccctg gctgctggta atacatcccc acctggtcct gcgcaatcgg cactgtcaga
     4141 attcggtgcg tcacatcacc cagtgtctct ttacgctcgg aaagacgaat tccattttct
     4201 ttggtttcgt ttttcggcac ttcaaagaat ccgctggtcg cttccccacc attacggcgt
     4261 gagaaggtct gaacgaaatc gctcggttcc acgtttgagt aggtgaccgg cagcgcgttt
     4321 tcacacgcct ggccggaact gcatcccggt tgaatcgtca gctcttttga gaccggatcg
     4381 taagtgactt tatcgcccgg taaacccacc gcgcgcttga tgtaatcaag ctttggatct
     4441 tccggatatt taaagaccac gatatcgccg cgtttcggat gaccggtttc gatcagcgtt
     4501 ttctggtaga taggatcttt aatgccataa gcaaacttct ctaccagaat aaaatcaccg
     4561 attaacagag tcggcatcat cgaacctgac gggatctgga acggttcata aataaacgaa
     4621 cgtacaatca atacgatagc cagcaccgga aaaacagaag caccggtttc cagccagcca
     4681 ggcttcggcg caaccttttt cagggttgct ttatccagtg agtcaccggc agccgcctgc
     4741 gccgctgcct gacgttcccg ccgtttaggt gcgaaaaaga atttatccac gcaccataaa
     4801 atgcccgtca ccagtgtggc aatcaccaga atcagggcaa acatattcgc catgccaact
     4861 cctaagggtt atttgttgtc tttgccgacg tgcagaatgg cgaggaacgc ttcctgcggc
     4921 agctcgacgt taccgatctg cttcatgcgt ttcttacctt ctttctgctt ctgcagcagc
     4981 tttttcttac ggctgatatc gccgccataa catttagcca gtacgttttt acgcagctgt
     5041 ttcacggtgg atcgcgcaat aatgtgcgta ccaatcgctg cctgaatggc gatatcaaac
     5101 tgctggcgtg ggatcagatc tttcatcttc tccaccaact cgcgaccgcg gttttgcgaa
     5161 ttatcacggt gggtgatcag cgccaacgca tcaacacgtt caccgttgat taatacgtct
     5221 acacgtacca tgtcggacgc ctggaagcgt ttgaagttgt aatccagaga cgcataacca
     5281 cgcgaggtag atttcaggcg atcgaagaag tcgagcacca cttccgccat cgggatctcg
     5341 tacgtcagcg ccacctgatt accgtggtaa accatattgg tctgcacgcc gcgtttttct
     5401 acgcacagcg taataacgtt gccgagatat gcctgcggca gcagcatgtg acactctgca
     5461 atcggctcgc gcagttcgta aatgttattt accgcaggca gcttggatgg gctgtcgacg
     5521 tagataactt cccttgacgt ggtttcaact tcatacacta cggtcggcgc agtggtgatc
     5581 agatccagat cgtattcacg ttccagacgt tcctggatga tctccatgtg cagcaggcca
     5641 aggaagccgc agcggaaacc aaagcccagc gcgctggagc tttccggctc atagaacagt
     5701 gaggcatcat tcaggctgag tttacccagc gcgtcacgga aggcttcata gtcgtcggaa
     5761 cttaccggga acagaccggc gtatacctgc ggtttgactt tcttaaagcc aggcagcgcc
     5821 ttttctgccg gattacgcgc cagcgttaag gtatcgccga ctggagcgcc gtggatatct
     5881 ttaatcgcac ataccagcca gcctacttcg ccacatttca gttcagtgcg gtcaacctgt
     5941 ttcggcgtga agatgcccag acggtcggcg ttataggtct gcccggtact catgactttc
     6001 actttgtcgc ccttacgcag ggtgccgttt ttaatacgga taagtgaaac aacgcccagg
     6061 tagttgtcga accatgagtc gataattagt gcctgcaacg ggccttccgg atcgccttcc
     6121 ggcggcggaa tgtcgcgcac cagacgttcg agaacgtcct gcacgccaac gccggttttc
     6181 gctgaacagc gcaccgcgtc agtggcgtcg atgccgacga tatcttcaat ttcttccgcc
     6241 acgcgttcag gatcggctgc cggcaggtca atcttgttca gtactggcac cacttcgaga
     6301 tccatttcca tggcggtgta gcagtttgcc agggtttgcg cttccacgcc ctgcccggcg
     6361 tcgaccacca gcaatgcacc ttcacaggca gccagcgaac gggaaacttc ataggagaag
     6421 tctacgtggc ccggggtgtc gataaagtta agctgatagg tttcgccgtc agacgctttg
     6481 tagtccagcg tcacgctttg cgctttgatg gtaatgccac gctcacgctc aagatccatg
     6541 gaatcgagaa cctgcgcctc catttcacgg tcagacaggc caccgcagat ctggataata
     6601 cggtcagaca gcgtcgattt accgtggtca atgtgagcta tgatcgaaaa gttacgtata
     6661 ttcttcataa agtatgatta ttgtgcctta atgcccggtt aaccaggctt ttagaagtcg
     6721 ctgttctgag cttaacgtct gtattaatag aaacgccgca ttctacacta caacattgag
     6781 gcgaggaaat gttcataccg tatggattgt ggtatctgga aacgtcctcg catttgttat
     6841 gcaaaatgca acaaagccag tgaaatcact ggctcgcgtc ttccgaagat gtttcaaatc
     6901 gcacaaggcc aggcggcaat gccacgctta agatgatcgg ttgccattct gcccgggcag
     6961 caaacttacg cgagtagccg cgcgcaatca ggaacccgcc aataccgccg aggaccgcac
     7021 cacataatgc tgcgacgtca gaagcaaaga gtagctgaaa tagcgaagcg ataaggaata
     7081 atcccaccag cggcgacata taaaccagta atgcggagct aagcaggctg ccttcggcga
     7141 tccccaattc cactttttgc cccggcacta acggttcatc acagggtacg acaatggtat
     7201 gcgtggtttg cgggccaagt ttattcaaca cgcggctacc gcaaccggcg cgtgaagcgc
     7261 agctgctgca tgaagcttta acatcacaac tgaccagcgc ctgcccgttt tgccaggaga
     7321 cgacggtagc ccactctttg atcattgcgc tgccccgaac ttaatattct cggcaatgcg
     7381 tttcgccgtt tgcggcggca gttcaccgac aatggtgatt tcggcgttat cacgtacgct
     7441 tgtactgacg gttctgcgtc cggtgcgcaa catctgatcg gtgctcgatg gcgtagcgcg
     7501 gttaacgttt accgagaagc tgaataatcc gtcagaatag agacgtgatt cgataggcat
     7561 gttgtccatc gtcggtagcg gacgtcgact actggaaact tcgctaaaac cctgtggcaa
     7621 ccaggttggc gtccagctga atttagcttt ttcacctaca ggaacagaaa gcaacggcgg
     7681 caaatttgcc ttcgccagcg tctgcatact gctgctgata tcctgattga cgttaaaagc
     7741 aatcacgcga aattgttcca gcgtttcacc atcgcgatca aggagatcaa cccgcatcgg
     7801 taatttcgat tcggtgtcca tccacacgat gtagctgtag cgtgtaccat ctcgggcaac
     7861 cacgcgaatg acttcgcaaa gacgatcagc aatacgcgtg cgcccgacgg agataaagtc
     7921 gtagtaagga gaaaggcgtt tgaaatcggt atagatgagc gatggcagag aatcaacaat
     7981 gtaatcgcca ttaagcgtga acggttcaag tcccggttca aaatagctga tttcattgcc
     8041 gcgctgtacc acttcccggc gcgggccatc catttgcaac aattgtgcaa gaggacggtt
     8101 atcgaggcgt gcatgtcgat aacgcagaga ctcaacaccc tgtttattga tgctgatgaa
     8161 tgacagctcg taattcagtg actgactggc caggttcatc tgctgtaata acgccccgga
     8221 cgcgggagtg gccgaggcgt tagcagagaa taacaggcta cctgtcacta atgacatggc
     8281 aaaccaaagt tgcttcatta ctgcgattgc gttcctaaag tttgaattcc tggcacctgt
     8341 acagcggctt gctgggtttg cgcctgctca aactgaagct gttcagagtg gagtcggcgt
     8401 tgcagttcgt aatcctgcaa cattgcatta atgcgacgac gctgctcctg tacctgctgc
     8461 tgttgaccat tgtttgcggt cgcttcagaa ggtactccca ggcttaccgg gctggcttta
     8521 cccatcatcg gcagtgtatt aaataccggc gtttcgggct gctgggacgt ttcagattgt
     8581 ccattatagt gctggacgcc aacgataact gcaagcgata cgcatgctgc tacgcccatt
     8641 tgggtaagct gtgccgccca cggacgtact ttctgccaga atggcatttt ctgccattga
     8701 tgcggcgcag gctgggcttc cgggatcaat gtcgccggtt gacgtactgg ctcttcttca
     8761 atggcggcca tcacgcgtga agagatatcg aaatggagca cctcgggagt atcaccccgc
     8821 attgagtcac ggattaagtg atagctttcc caggttttct gcatttctgg gttatgagcc
     8881 agttcgttaa gcagctcact atccagcgtt tcgccatcca ttaaagcgga aagttgttct
     8941 ttctgcatgc ctaataccct tatccagtat cccgctatcg tcaacgcctg ataagcggtt
     9001 gaactttgtt atcaatagct tccctcgctc ggaagatacg tgaacgcacc gtacctaccg
     9061 gacaatccat gatagcggct atctcttcat agctcaggcc atccagctcc cgcaaggtta
     9121 ttgccatgcg caaatcttcc gggagggact caatagttcg gaaaactatc tgtctcagtt
     9181 cttctgacaa cattaagttc tcagggttcg aaatttcttt caacgcgccg ccactttcga
     9241 agttttcagc ttcaatggca tccacatcac tggaaggtgg acgacgcccc tgagcaacca
     9301 ggtaattttt cgctgtattt acagcaatcc gatacagcca tgtataaaaa gcgctatctc
     9361 cccggaacga atccagcgca cgataggctt taataaaagc ttcttgtacc acatcgggaa
     9421 catcacccga cggcacatag cgggaaacca gactcgccac tttatgctga tagcgcacta
     9481 ccagtaagtt aaaggctttc tgatctccct tctggacccg ttcaaccagg acctggtccg
     9541 ttaactgctc gctcatccga ggtaaagtct ccccaaacca aatttccacg cgctatcgaa
     9601 acaccactcc attagctgca atttgagcaa gcaaagggtt agagtgtctc gtttttgtaa
     9661 agttccgtaa cgcatctgtt tttgtttgtc atgctgtaga cggatcatta tctatcatta
     9721 taagtctaca gaatctgaac atcgcattct ctgtgtagaa atgcccattt aactgcctga
     9781 agagtaaccc aacggccttt ttatttcacc acctaatcct ccaccagcca gtaacttctc
     9841 tttttctcgc cgccctgcgt cagcgtgttt agccactgta acaaatatta aaatagcagg
     9901 tgtttattcg cacaacatga tgctatgctg accaaacggt gtttagtaaa ttaaacaaag
     9961 aaaatgaata ctctccctga acattcatgt gacgtgttga ttattggtag cggcgcagcc
    10021 ggactttcac tggcgctacg cctggctgac cagcatcagg tcatcgttct aagtaaaggc
    10081 ccggtaacgg aaggttcaac attttatgcc cagggcggta ttgccgccgt gtttgatgaa
    10141 actgacagca ttgactcgca tgtggaagac acattgattg ccggggctgg tatttgcgat
    10201 cgccatgcag ttgaatttgt cgccagcaat gcacgatcct gtgtgcaatg gttaattgac
    10261 cagggggttt tgtttgatac ccacgttcaa ccgaatggcg aagaaagcta ccatctgacc
    10321 cgtgaaggtg gacatagtca ccgtcgtatt cttcatgccg ccgacgccac cggtagagaa
    10381 gtacaatcca cgctggtgag caaggcgcag aaccatccga atattcgtgt gctggagcgc
    10441 agcaacgcgg ttgatctgat tgtctctgac aaaattggcc tgccgggcac gcgacgggtt
    10501 attggcgcgt gggtatggaa ccgaaataaa gaaacggtgg aaacctgcca cgcaaaagca
    10561 gtggtactgg caaccggcgg tgcgtcgaag gtttatcagt acaccaccaa tccggatatt
    10621 tcttctggcg atggcattgc tatggcgtgg cgcgcgggct gtcgggttgc caatctcgaa
    10681 tttaatcagt tccaccctac cgcgctgtat cacccacagg cacgcaattt cctgttaacg
    10741 gaagcactgc gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg ttttatgccc
    10801 gattttgatg agcgcggcga actggccccg cgcgatattg tcgcccgcgc cattgaccat
    10861 gaaatgaaac gcctcggcgc agattgtatg ttcctcgaca tcagccataa gcccgccgat
    10921 tttattcgcc agcatttccc gatgatttat gaaaaattgc tcgggctggg gattgatctc
    10981 acacaagaac cggtaccgat tgtgcctgct gcacattata cctgcggtgg tgtaatggtt
    11041 gatgatcatg ggcgtacgga cgtcgagggc ttgtatgcca ttggcgaggt gagttatacc
    11101 ggcttacacg gcgctaaccg catggcctcg aattcattgc tggagtgtct ggtctatggc
    11161 tggtcggcgg cagaagatat caccagacgt atgcctgatg cccacggcgt cagtacgtta
    11221 ccgccgtggg atgaaagccg cgttgagaac cctgacgaac gggtagtaat tcagcataac
    11281 tggcacgagc tacggctgtt tatgtgggat tacgttggca ttgtgcgcac aacgaagcgc
    11341 ctggaacgcg ccctgcggcg gataaccatg ctccaacaag aaatagacga atattacgcc
    11401 catttccgcg tctcaaataa tttgctggag ctgcgtaatc tggtacaggt tgccgagttg
    11461 attgttcgct gcgcgatgat gcgcaaagaa agccgggggc tgcattttac gctggattac
    11521 ccggaactgc tcacgcattc cggtccatcg atcctttccc ccggcaatca ttacataaac
    11581 agataaaaag cctgggtcag cgccgtatac gcttcggaat agttctggtc tggcccacga
    11641 atgactaagc gatcgctaaa gcattctccc gcctgcgggg agaatgccag cagcacccga
    11701 tgcggcagtc gcgcttcgtt ttccgccaca tccgtccgca aacgtaaatg ccagcccatg
    11761 cttaatgcca gttcagtaaa accattacca atctgctctg gcagcaccac gcagaaaaat
    11821 ccctcttcgg taatgcactc cgccgcacag gtcagcagcg atgggtgatc aagcgtagtg
    11881 gtatagcgag cctgttcccg ttgaggtgtc gcgcactcta ctccctgctg atagtaaggt
    11941 gggttactga tgattaaatc gaagcatact gtctgctgtg tgatccactg ctgaatatcc
    12001 gccgtatgga cgttaatccg ctctgcccac ggggactggt tgatattttc ctgcgcctgc
    12061 gcggcagctt cactttccag ctcaacggca tcaattatca cgctgtcact ggttcgctgc
    12121 gccagcatca gtgccagcaa cccgctaccc gcgccgatat caaggcaacg tttcacccca
    12181 gccaccggtg cccatgcgcc cagcaaaatg ccatccgttc ccactttcat cgcacagcga
    12241 tcgtgagcaa caaaaaactg tttaaaagta aatccattac gacgaagcac ggatgtagac
    12301 tgtgacatga aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg agaacaatac
    12361 ccgagaagcg atatccatac aaacagatga agattgcagc cgtaacgtct ataatcagcg
    12421 ccccacacag aggtagaaca tgactgtaac gactttttcc gaacttgaac tcgacgaaag
    12481 cctgctggaa gccctccagg ataaaggttt cactcgcccg accgccattc aggctgccgc
    12541 cattccgcct gcgctcgatg gccgtgatgt actcggttct gcgccgacag gcaccggtaa
    12601 aacggcggcg tatctgctgc cagcgttgca gcacctgctc gatttcccgc gtaagaaatc
    12661 cggtccgccg cgtattttga tcctcacccc aactcgcgag ctggcgatgc aggtggccga
    12721 tcatgcccgc gaactggcga aacatacgca tctggatatc gccaccatca ccggcggcgt
    12781 agcctatatg aaccacgcgg aagtgttcag cgaaaatcag gacatcgtgg tcgccacgac
    12841 cggacgtctg ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg ttgaaacgct
    12901 gatcctcgac gaagcagacc gtatgctgga tatgggcttc gctcaggata tcgaacatat
    12961 tgctggcgaa acgcgctggc gtaaacagac cctgctcttt tcggcaacgc tggaaggcga
    13021 tgcgattcag gactttgccg agcgtctgct ggaagatccg gtggaggttt ctgccaatcc
    13081 gtccacccgt gagcgcaaaa aaattcatca gtggtattac cgcgccgatg acctggaaca
    13141 taaaaccgca ttgctggtgc atctgttaaa acagccggaa gcgacccgct caattgtgtt
    13201 tgtgcgtaag cgtgaacgtg tgcatgagct ggcaaactgg ctgcgcgaag cgggcatcaa
    13261 caactgctat ctcgaaggtg agatggtaca aggcaagcgt aacgaagcga tcaagcgttt
    13321 gaccgaaggt cgcgtaaacg tactggttgc aaccgatgtt gccgcgcgcg gtatcgacat
    13381 tcctgacgtc agccacgtct ttaacttcga tatgccgcgc agtggcgata cttatttgca
    13441 ccgtatcgga cgtaccgcgc gcgccggtcg taaaggcacc gcaatttcgt tggtggaagc
    13501 ccatgaccat ttgctgctgg gcaaggttgg ccgttacatt gaagagccga ttaaagctcg
    13561 cgttattgat gagttacgcc cgaaaacgcg tgcgccaagc gagaagcaga ccggcaagcc
    13621 atcgaagaaa gtactggcaa aacgcgccga gaagaaaaaa gctaaagaga aagagaaacc
    13681 gcgggtgaaa aaacgccatc gcgacaccaa aaatattggt aagcgccgta aaccaagcgg
    13741 aacgggcgtg ccaccgcaaa cgacagaaga gtaatctcaa tgccgggttt aagcccggca
    13801 ttatttttat cccggcaaaa aacactcttc aacacactga ataaaagtgt gcatcgccgg
    13861 gctaaccgct tttccagcat ggtgtgcgca catggccgta atggtctgcg actgttcgcc
    13921 aaaaggcaat tcaattaatt ctccgctctc aagctctttt tccaccgcaa agcggggcaa
    13981 gtaactgaca ccgatattcg ccgcgacaca acgtttgata ctttcaatgc ttaacagctc
    14041 aatggtgttt tccaccgtga tccgccgctg acgcagcgtg ctctcaaata tctgccggaa
    14101 gacacattgc ggttcgttga taataaagct acaggcgtta tgtcttcccg gctcagtaaa
    14161 atcgacatct gcaatttgcg gtgaagccac cagcaccagc gattgttcac ccaactctcg
    14221 tcggttcagg gcatcatcat tccctacgcg ataaaagacg ccgacatcgg cctcatcatt
    14281 cagtagcgca tcacggatca cgtaacagtt cagcgactgc aacgataaac gcacttttgg
    14341 tgcgcgttgc cgaaaacgct gcaacacctg cggcattcgg taagagagca acatttcgcc
    14401 cgaaacaacg cgtagttccc cgtccggatc tgactccttt ttggcagcct cacgaagcgt
    14461 atccatcact cgggtaagtt cataaatgtg cggcagcaac ttttttcctt cgcgggtaag
    14521 gcacatccgt cggccaattt tctcaaataa ctggactgaa aacccctgct caagctgctg
    14581 aatatgaaaa gtcaccgtcg attgtgtaca gcacaatttt tgcgaagctc gcaaaaagga
    14641 accctcttcc accacggttt taagcgtaat aaaacggcgc agatccataa ccccaaacct
    14701 atcgaaaata tcgaatctag aatataaaaa cattcatttt tttaaatgtt ccgtgtcggg
    14761 tactgtctac caaaacagag gagataacaa gtgacaccga cccttttaag tgctttttgg
    14821 acttacaccc tgattaccgc tatgacgcca ggaccgaaca atattctcgc ccttagctct
    14881 gctacgtcgc atggatttcg tcaaagtacc cgcgtgctgg cagggatgag tctgggattt
    14941 ttgattgtga tgttactgtg tgcgggcatt tcattttcac tggcagtgat tgacccggca
    15001 gcggtacacc ttttgagttg ggcgggggcg gcatatattg tctggctggc gtggaaaatc
    15061 gccaccagcc caacaaagga agacggactt caggcaaaac caatcagctt ttgggccagc
    15121 tttgctttgc agtttgtgaa cgtcaaaatc attttgtacg gtgttacggc actgtcgacg
    15181 tttgttctgc cgcaaacaca ggcgctaagc tgggtagttg gcgtcagcgt tttgctggcg
    15241 atgattggga cgtttggcaa tgtatgctgg gcgctggcgg ggcatctgtt tcagcgattg
    15301 tttcgccagt atggtcgcca gttaaatatc gtgcttgccc tgttgctggt ctattgcgcg
    15361 gtacgcattt tctattaacg aaaaaaagcg gaagaggtcg ccctcttccg cttagtaact
    15421 tgctacttaa gccttacagg ctttcagtaa aggtacgagc gataacgtcg cgctgctgtt
    15481 ccggagtcag agagttaaag cgaactgcat aaccggatac acggatggtc agctgcggat
    15541 atttttccgg atgcttaact gcatcttcca gagtttcacg acgcagaacg ttaacgttca
    15601 ggtgttgacc accttcaacg cgaacttctg gtttcacttc tactggaact tcacggtatt
    15661 caatgtcacc cagtttgctt actgcaacca cttcatcttc tgcataacct gcttttgcaa
    15721 cgatgcaacg tgcttcgcct ttttcgctgt ccagcagcca gaaagagttc agcagatcgt
    15781 cgttagcggc tttagtaatc tggatacctg taatcatgtg atgcctcccc ggcaaaatta
    15841 tttgatttgt tcagcctgtc gcggccaatt ggtaaaacca ttgttgcttg agtgtatata
    15901 tactcctcaa ccacccttga ttctttgatt taaatcaata aaaaccacac atcgattgtg
    15961 gtcgtaaatg gattttattg ttttacatca acttatgcgg gtgtgaaatt ttaccaattt
    16021 acattttttt gcactcgttt aagtctaaaa aatgagcatg attttgttct gtagaaagaa
    16081 gcagttaagc taggcggatt gaagattcgc aggagagcga gatggctaac gaattaacct
    16141 ggcatgacgt gctggctgaa gagaagcaac aaccttattt tcttaatacc cttcagaccg
    16201 tcgccagcga gcggcagtcc ggcgtcacta tctatccacc acaaaaagat gtctttaacg
    16261 cgttccgctt taccgagttg ggtgacgtta aagtggtgat tctcggccag gatccttatc
    16321 acggaccggg acaggcgcat ggtctggcat tttccgttcg tcccggcatt gccattcctc
    16381 cgtcattatt gaatatgtat aaagagctgg aaaatactat tccgggcttc acccgcccta
    16441 atcatggcta tctcgaaagc tgggcgcgtc agggcgttct gctgctcaat actgtgttga
    16501 cggttcgcgc aggtcaggcg cattcccacg ccagcctcgg ttgggagacg tttactgata
    16561 aagtcatcag cctgattaac cagcaccgcg aaggcgtggt gtttttgttg tggggatcac
    16621 atgcgcaaaa gaaaggggcg attatagata agcaacgcca tcatgtactg aaagcaccgc
    16681 atccgtcgcc gctttcggcg catcgtggat tctttggctg caaccatttt gtgctggcaa
    16741 atcagtggct ggaacaacgt ggcgagacgc cgattgactg gatgccagta ttaccggcag
    16801 agagtgagta aatttgcggt aaaatgccgg atggcagagt tgccacccgg ctgatttatc
    16861 aggctttatt ctgacgccac cattcaccaa gcaaaacgcc ggttgcaaca gagatattca
    16921 gcccggcaac gttgcccgta ccgtcaatct tcacgcgcag atcgttcgga tcgcgtgcgg
    16981 catccggtaa cccttcatat tcctgaccca gcaccagtac cattttcgct ggcagactgg
    17041 ttttgaacag cggtttcccc tgctcgctgg aagtggtcac tacggtgtaa cctgcctgac
    17101 ggaaatcatc caacacgtta acaatgttgt cgccagtaat cggctgaacg tgctctgcgc
    17161 cgccttctgc ggtacggata gccgcccccg actccagcag tgccgcatcc tgcaccacaa
    17221 cgcctttcac gccgaagtgc gcgcagctgc gcatcatgcc acccaggtta tgcgggttag
    17281 attcgttttc cagcgccagt acacaatcct gcgcgcctgc ctggcttacc cactgctgca
    17341 cggttgtgcc attacgcttt ttgatcagga agcaaacgcc gccgtgatgt tccgtgcctg
    17401 acgcttttgt caattccgct tcatccacca catggtacgc tttgcggttt gctgccatcc
    17461 agcgcaaggc ttctttaaaa cgcggcgtta cactctggat aaaccaggcg cgaacaatcg
    17521 cttccggacg gctctggaaa agtgcctgac atgcattttc gccgtagacg cgggtttctt
    17581 ccgtacgctg acgacgcaac acttccggat caataaaact tttaccactg atgccaccgt
    17641 gatcggcctt ttccggcgtc tcatcacccg gcgcgcggga aaccgtgcgc cacggcgaat
    17701 cttcccactt gcggtcacgg ggctgactct gtttgtcatc gcgggcgggg cgacggccac
    17761 cgtcagcacg agattttcct ggacgcccgc cccctttccc ggtacgcggg ttgtgggtac
    17821 gtttatcaga atcatcatca ctgcggacat acatcacttt gaccttgccg cttttacctt
    17881 tcatttcatc gttcatgctt ttctccacca gcgctgcgcg aagcgcgcag attacccgaa
    17941 gtccgcgcgg ttcgccatga tttcgtacca aagcctgcga ctatcatacc tattgaataa
    18001 aacagattgt tgtctggaac aatgtccccg ataatatgta acatattaga aacataccgg
    18061 cgtcgttgcc gataagtctc cttactcatc ccgaggttag ttatgaatac cgtttgtacc
    18121 cattgtcagg ccatcaatcg cattcccgac gatcggatcg aagatgcggc aaaatgcgga
    18181 cgctgcggtc acgacttgtt tgacggagag gtgattaatg cgaccggtga aacactcgac
    18241 aaattgctga aggatgatct acctgtggtg atcgacttct gggcaccgtg gtgcggcccc
    18301 tgccgtaatt tcgcaccaat ttttgaagat gtcgcgcaag agcgtagcgg taaagtgcgc
    18361 tttgtgaaag tgaataccga agctgaacgt gaattgagca gtcgctttgg aattcgtagt
    18421 ataccgacga tcatgatttt caaaaacggt caggttgtcg acatgcttaa tggcgcagta
    18481 ccgaaagcgc cgttcgatag ctggctgaac gaatctcttt aatcttaccg gggcgcatct
    18541 tgtgccccgt tttctcctct gcgacaatgg cgttttttcg acgctctctt atgaccgaaa
    18601 acgctgttct ccagttacgc gccgagcgta ttgcgcgcgc aacacgtcct tttcttgccc
    18661 gcggtaatcg cgttcgtcgc tgccaacgct gtcttttgcc agagaaatta tgtctctgtt
    18721 cgacaattac cccagcacaa gcaaaaagtc gcttctgttt gctgatgttc gacaccgagc
    18781 caatgaagcc cagtaatacc gggcgtctga ttgctgatat tttgcctgat accgttgcgt
    18841 ttcaatggtc gcgtaccgaa ccctcgcagg atttgctgga tctggtacaa aacccggatt
    18901 atcagccaat ggtggtcttt cccgcctcgt atgctgatga gcaacgggaa gtgatcttca
    18961 caccacctgc cggtaagcca ccgctgttta tcatgctcga tggtacctgg ccggaagctc
    19021 gcaagatgtt tcgtaaaagt ccgtatctgg ataatcttcc cgtcatttcc gtcgatcttt
    19081 cccggctttc tgcctatcgc ctgcgtgaag cccaggctga aggccaatat tgtactgccg
    19141 aggtagccat cgcactgtta gatatggccg gcgataccgg ggcggcagca gggttaggcg
    19201 agcattttac ccgctttaaa acacgctatc tggcaggaaa aacgcaacat ctgggtagca
    19261 tcacagcaga acagttagaa agcgtttaaa atcattcggt cacttctgcg ggagaccggt
    19321 atgagtcagc gaggactgga agcactactg cgaccaaaat cgatagcggt aattggcgcg
    19381 tcgatgaaac ccaatcgcgc aggttacctg atgatgcgta acctgctggc gggaggcttt
    19441 aacggaccgg tactcccggt gacgccagcc tggaaagcgg tgttgggtgt gttggcctgg
    19501 ccggatattg ccagcttgcc ctttacaccc gaccttgcgg ttttatgtac caatgccagc
    19561 cgtaatcttg ctcttctgga agagctcggc gagaaaggct gtaaaacctg cattattctt
    19621 tccgccccgg catcgcaaca cgaagatctc cgcgcctgcg ccctgcgcca taacatgcgc
    19681 ctgcttggac caaacagtct gggtttactg gctccctggc aaggtctgaa tgccagcttt
    19741 tcgcctgtgc cgattaaacg cggcaagctg gcgtttattt cacaatcggc agccgtctcc
    19801 aacaccatcc tcgactgggc gcaacagcgt gagatgggct tttcctactt tattgcgctc
    19861 ggcgacagcc tggatatcga cgttgatgaa ttgctcgact atctggcgcg cgacagtaaa
    19921 accagcgcca tcctgctcta tctcgaacag ttaagcgacg cgcgacgctt tgtttcggca
    19981 gcccgtagtg cctcgcgtaa taaaccgatt ctggtgatta aaagcggacg tagcccggcg
    20041 gcacagcgac tgctcaacac gacggcagga atggacccgg catgggatgc ggctattcag
    20101 cgtgccggtt tgttgcgggt acaggatacc cacgaactgt tttcggcggt ggaaaccctt
    20161 agccatatgc gcccgctgcg tggcgaccgg ttgatgatta tcagcaacgg tgctgcgcct
    20221 gccgcgctgg cgctggatgc cttatggtca cgcaatggca agctggcaac gctaagcgaa
    20281 gaaacctgcc agaaattgcg cgatgcattg ccagaacatg tggcagtctc taaccctctc
    20341 gatctgcgcg atgacgccag cagcgagcat tatgtcaaaa cgctggacat tctgctccac
    20401 agccaggatt ttgatgcgct gatggttatt cattcgccca gcaccgctgc tcccgcaaca
    20461 gaaagcgcgc aagtattaat tgaagcggta aagcatcatc cccgcagcaa atatgtctct
    20521 ttgctgacga actggtgcgg cgagcactcc tcgcaagagg cacgacgttt attcagcgaa
    20581 gccgggctgc cgacctaccg taccccggaa ggaaccatca ctgcttttat gcatatggtg
    20641 gagtaccggc gtaatcagaa gcaactacgc gaaacgccgg cgttgcccag caatctgact
    20701 tccaataccg cagaagcgca tcttctgttg caacaggcga ttgccgaagg ggctacgtcg
    20761 ctcgataccc atgaagttca gcccatcctg caagcgtatg gcatgaacac gctccctacc
    20821 tggattgcca gcgatagcac cgaagcggtg catattgccg aacagattgg ttatccggtg
    20881 gcgctgaaat tgcgttcgcc ggatattcca cataaatcgg aagttcaggg catcatgctt
    20941 tacctgcgta cagccaatga agtccagcaa gcggcgaacg ctattttcga tcgcgtaaaa
    21001 atggcctggc cacaggcgcg ggtccacggc ctgttggtgc aaagtatggc taaccgtgct
    21061 ggcgctcagg agttgcgggt tgtggttgag cacgatccgg ttttcgggcc gttgatcatg
    21121 ctgggtgaag gcggtgtgga gtggcgtcct gaagatcaag ccgtcgtcgc actgccgccg
    21181 ctgaacatga acctggcccg ctatctggtt attcagggga tcaaaagtaa aaagattcgt
    21241 gcgcgcagtg cgctacgccc attggatgtt gctggcttga gccagcttct ggtgcaggtt
    21301 tccaacttga ttgtcgattg cccggaaatt cagcgtctgg atattcatcc tttgctggct
    21361 tctggcagtg aatttaccgc gctggatgtc acgctggata tctcgccgtt tgaaggcgat
    21421 aatgagagtc ggctggcagt gcgcccttat ccgcatcagc tggaagaatg ggtagaattg
    21481 aaaaacggtg aacgctgctt gttccgcccg attttgccag aagatgagcc acaacttcag
    21541 caattcattt cgcgagtcac caaagaagat ctttattacc gctactttag cgagatcaac
    21601 gaatttaccc atgaagattt agccaacatg acacagatcg actacgatcg ggaaatggcg
    21661 tttgtagcgg tacgacgtat tgatcaaacg gaagagatcc tcggcgtcac gcgtgcgatt
    21721 tccgatcctg ataacatcga tgccgaattt gctgtgctgg ttcgctcgga tctcaaaggg
    21781 ttaggcttag gtcgacgctt aatggaaaag ttgattacct atacgcgaga tcacggacta
    21841 caacgtctga atggtattac gatgccaaac aatcgtggca tggtggcgct agcccgcaag
    21901 ctcgggttta acgttgatat ccagctcgaa gaggggatcg ttgggcttac gctaaatctt
    21961 gcccaacgcg aggaatcatg agtaaggtac tggaaatgtt gaccacttaa tcgggactgg
    22021 tgttattatt acccgcttat gtcgtctgca ttgcacagag gacccttcaa tgaacagaga
    22081 agaaatgcac tgtgatgttg tcaaaattta agcgtaataa acatcaacaa caccttgccc
    22141 aactacccaa gatttctcaa tcagttgatg atgtcgattt cttttacgct cccgccgact
    22201 tccgggagac gctgctggaa aaaatagcca gcgcgaagca gcgcatttgc attgtcgccc
    22261 tgtatctcga acaggatgac ggtggcaaag gcattctgaa cgcgttgtat gaagctaaaa
    22321 ggcagcgtcc ggaactggat gtgcgggtgc tggtcgactg gcatcgtgca caacgtggac
    22381 gcattggcgc tgcggcatct aacactaacg ctgactggta ctgccgcatg gcgcaggaaa
    22441 atccgggcat agatgttccg gtttatggcg ttccaatcaa tacccgtgaa gcccttggtg
    22501 ttctgcactt taaaggcttt atcatcgacg atagcgtact ttatagcggt gccagcctga
    22561 acgatgttta cctgcatcag cacgataaat atcgctacga ccgttatcat cttatccgta
    22621 accgtaagat gtcagacatt atgtttgaat gggttacaca gaatattatg aatggccgcg
    22681 gcgttaatcg tctggatgat gttaatcggc caaaaagccc ggaaatcaag aacgatattc
    22741 gtctgttccg ccaggagctg cgtgatgccg cttatcattt ccagggcgat gccgacaacg
    22801 atcagctttc cgtaacgccg ctagtggggc tggggaaatc gagtctgttg aacaagacca
    22861 ttttccatct tatgccttgt gccgagcaga aactaaccat ctgtacgcca tacttcaacc
    22921 tgccagcaat ccttgtgcgc aatattatcc agttgctgcg cgaagggaaa aaggtcgaaa
    22981 ttattgttgg tgataaaacc gcgaacgact tctacattcc ggaagatgaa cctttcaaga
    23041 taattggcgc attgccttat ctctatgaga tcaatctgcg tcgtttcctg agccgtttgc
    23101 agtattacgt caatactgac cagctagtgg ttcggttatg gaaagatgac gacaacacct
    23161 atcacctgaa agggatgtgg gttgatgata agtggatgtt gatcaccggt aataacctga
    23221 acccgcgcgc ctggcgtctg gatctggaaa acgccatttt gatccacgat ccacaactgg
    23281 agcttgcgcc gcagcgagag aaagaactgg agctgatccg cgagcatacc accatcgtta
    23341 agcactatcg cgatctgcaa agtattgccg attatccggt gaaggttcgt aaactcatcc
    23401 gccgtttgcg ccgtatccgc atcgaccgat taattagccg catcctgtaa tcacaacccc
    23461 gtcctgtacg gggtttgttt tttggaggcc acgttttgcg tattcttttt gtctgttcgc
    23521 tattgttact ttctggatgc agccatatgg ctaacgatag ctggagcggg caggataaag
    23581 ctcaacactt tatcgcctcg gcgatgcttt ccgccgccgg aaatgaatat tcacagcatc
    23641 aggggatgag ccgggatcgc agtgccatgt ttggattgat gttctctgtc agtttggggg
    23701 cgtcaaaaga gctttgggat agccgccccg aagggagcgg ctggagctgg aaggatttgg
    23761 cctgggatgt cgccggtgca agcaccggct ataccgtctg gcaactgacc cgtcactaaa
    23821 gacgcatccc cttccctttg cgatgtagca tcaaagaaac cagaaacgcc accacggcca
    23881 tcaaggtcac ataccagaag aaggctgttt ccattcctat tgatttcagc gacaacgcta
    23941 cgtactccgc cgaaccacca aatatagcat tagcgaccgc atatgacagg ccaacgccta
    24001 atgcgcgaac ctgtgccggg aacatctcag ccttcagtat tccactgatt gatgtataaa
    24061 aactcactat cagcagggca cacatcacca gaccaaaagc ggcataaggc gaggaaacgt
    24121 tttgcaatgc tgagagaata ggaacggtaa aaatggctgc cagcgaaccg aaacataaca
    24181 ttgaggtacg gcgaccaatc ttatccgaca gcgcgccaat gagtggttga ataagcatga
    24241 atacaaacaa tgcggcagtc ataatgccac tcgccacgtt ggcatgcatt cccgcagtat
    24301 ttaccagata cttctgcata taagtggtga aggtatagaa acaaagggag cccgcagcgg
    24361 taaaaccgag aatcatgatg aatgcacggc gattgcgcca taatcctttc agagatccag
    24421 cttcttttaa agcgcgcgtt tcttgttgcg aagtttcatc taactgacga cgtaaccaca
    24481 acgccacaac agctaacaca gctcctaacg cgaaaggaat acgccatccc cactctctga
    24541 gtgcagcgtc ttccatggtg tgttgtaaaa ccacgacaac cagtagggct agcagttgtc
    24601 cgccgatcaa cgtcacatac tgaaatgatg cgtaaaaacc tttgcgccct tcaacggcaa
    24661 cttcactcat ataggtggcg ctggtgccat attctccgcc aacagataat ccctgaaata
    24721 aacgcgcgag aagcaataat gccggagccc acgtacctat agtttcataa cctgggaggc
    24781 aggcgataac cagcgatccg aaacacatca tacacaccga taacagcatc gattttttgc
    24841 gaccatgttt atcggctatg cggccaaata gccaaccgcc tattgggcgc atcaggaatc
    24901 ccgcagcaaa aacacctgct gtttgtagta gttgagtcgt cgtgttcccg gaagggaaga
    24961 aaatgtgggc aaagtagagt gaacagaacg agtagacata gaaatcgaac cactcgacca
    25021 gattacctga agaggccccc acaatcgccc aaatgcggcg acgagtatca ctacttgtca
    25081 gtttgctgtc tgccgttaca gtactttcag ccatgccatt atgtctcctg ccgtaatccg
    25141 atgcttttgt cggtcgcttt tgtttatttc ttttggtaaa gaaaatgtta tacatttgtt
    25201 gcgtatcatt atgcaacctt aaccatgaat ttagttagca ggaaaatggt tattgaggag
    25261 cttaaggata aatttctggt gaggagaaca cgtatggagg tgggcaagtt ggggaagccg
    25321 tattcgctgc tgaatctggc atatgtggga gtataagacg cgcagcgtcg catcaggcat
    25381 ttttttcacc aac
//
LOCUS       DAJSKJ010000043        25177 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000043 DAJSKJ010000000
VERSION     DAJSKJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.043"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(143..1024)
                     /gene="prmA"
                     /locus_tag="ON345_001506"
     CDS             complement(143..1024)
                     /gene="prmA"
                     /locus_tag="ON345_001506"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR3071590.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1036..2487)
                     /gene="panF"
                     /locus_tag="ON345_001507"
     CDS             complement(1036..2487)
                     /gene="panF"
                     /locus_tag="ON345_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR3071591.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAMTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2477..2719)
                     /locus_tag="ON345_001508"
     CDS             complement(2477..2719)
                     /locus_tag="ON345_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR3071592.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2828..4177)
                     /gene="accC"
                     /locus_tag="ON345_001509"
     CDS             complement(2828..4177)
                     /gene="accC"
                     /locus_tag="ON345_001509"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR3071593.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADHDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4188..4658)
                     /gene="accB"
                     /locus_tag="ON345_001510"
                     /gene_synonym="fabE"
     CDS             complement(4188..4658)
                     /gene="accB"
                     /locus_tag="ON345_001510"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR3071594.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5524..6354)
                     /locus_tag="ON345_001511"
     CDS             complement(5524..6354)
                     /locus_tag="ON345_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071595.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(6391..7365)
                     /locus_tag="ON345_001512"
     CDS             complement(6391..7365)
                     /locus_tag="ON345_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3071596.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            7517..9457
                     /gene="csrD"
                     /locus_tag="ON345_001513"
     CDS             7517..9457
                     /gene="csrD"
                     /locus_tag="ON345_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR3071597.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRSLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9762..10805
                     /gene="mreB"
                     /locus_tag="ON345_001514"
     CDS             9762..10805
                     /gene="mreB"
                     /locus_tag="ON345_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR3071598.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10871..11974
                     /gene="mreC"
                     /locus_tag="ON345_001515"
     CDS             10871..11974
                     /gene="mreC"
                     /locus_tag="ON345_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR3071599.1"
                     /translation="MKPIFSRSPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRTTPPQSGAQPPARAPGGQ"
     gene            11974..12462
                     /gene="mreD"
                     /locus_tag="ON345_001516"
     CDS             11974..12462
                     /gene="mreD"
                     /locus_tag="ON345_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR3071600.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMCKVRQQFA
                     VQ"
     gene            12471..13064
                     /gene="yhdE"
                     /locus_tag="ON345_001517"
     CDS             12471..13064
                     /gene="yhdE"
                     /locus_tag="ON345_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR3071601.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            13054..14523
                     /gene="rng"
                     /locus_tag="ON345_001518"
     CDS             13054..14523
                     /gene="rng"
                     /locus_tag="ON345_001518"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR3071602.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RISRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            14591..18391
                     /gene="yhdP"
                     /locus_tag="ON345_001519"
     CDS             14591..18391
                     /gene="yhdP"
                     /locus_tag="ON345_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR3071603.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNLGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            18547..19992
                     /gene="tldD"
                     /locus_tag="ON345_001520"
     CDS             18547..19992
                     /gene="tldD"
                     /locus_tag="ON345_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR3071604.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(20120..21049)
                     /gene="aaeR"
                     /locus_tag="ON345_001521"
     CDS             complement(20120..21049)
                     /gene="aaeR"
                     /locus_tag="ON345_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR3071605.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVVNSLTDYFVEVGKLFQEMHGRGKEK"
     gene            21232..21435
                     /locus_tag="ON345_001522"
     CDS             21232..21435
                     /locus_tag="ON345_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR3071606.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            21443..22375
                     /gene="aaeA"
                     /locus_tag="ON345_001523"
     CDS             21443..22375
                     /gene="aaeA"
                     /locus_tag="ON345_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR3071607.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            22381..24348
                     /gene="aaeB"
                     /locus_tag="ON345_001524"
     CDS             22381..24348
                     /gene="aaeB"
                     /locus_tag="ON345_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR3071608.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            24440..24712
                     /locus_tag="ON345_001525"
     CDS             24440..24712
                     /locus_tag="ON345_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR3071609.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24769..25032)
                     /gene="yhcN"
                     /locus_tag="ON345_001526"
     CDS             complement(24769..25032)
                     /gene="yhcN"
                     /locus_tag="ON345_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR3071610.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5971 a         6331 c         6791 g         6084 t
ORIGIN      
        1 aaagaaattt gagtaagttc tcaatttttc tttatgaatg aaaattttgg cacgcaaatt
       61 tgcgtaaata atcggcaaat ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg
      121 tcaccctacg gcgatgcgaa ggttaattct tacgaccggt aatacggcac cactcttctt
      181 tttccacgac cgggtccagt gcgaagctgt cggcataagc ttcacaaacg ctctctgcct
      241 ggcttgccag aataccggaa aggcccagca aaccgcctga aaccggcagg acgctgatta
      301 acggtgccag ttcacgtaat gggcctgcaa ggatgttagc gaccaccacg tcggctttca
      361 tttcttctgg ctgatctttc ggtaagtaga gttccagacg gtcagaaacg ccattacgtt
      421 cggcgttgtc gcggctggcc tgaatcgcct gcggatcgat atcaatacca atggcttttg
      481 ctgcacccag tttcagcgcc gcgatcgcca gaatgccgga accacagcca aagtcgatga
      541 ctgttttacc ggttaaatcg aggctgtcga gccattgcag gcacagagag gtggttggat
      601 gggtacccgt accaaacgcc agccctggat ctaacatcac gttgacggcg ttttcgtccg
      661 gcacatcacg ccagctaggg cagatccaca gtcgttcacc aaagcgcatc gggtggaaat
      721 tatccatcca ttcgcgctcc cagtctttat cttctagttg ttcgatttta tgcgcgaagc
      781 ctgcgccgag cagcggatgg ttttccagaa tcgccaccac gtcgttcata tcggtttcag
      841 cgtcgaacag accaatcaca tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct
      901 caaatactgg cgtatcgtgg gtatcctgaa aagtgataga aacggcaccc gcttccatca
      961 gcgcatcgct aagatcttcc gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag
     1021 gcatggcaaa actctttatt tatcagtagt caaaacggta gcttgcggga cggatgtacc
     1081 gaaacggttt ccgaccagga aagccagcaa acttagtagt aacgagggca cgatagggtg
     1141 gaagcccagg tactgaatat tcagcgtcgc gagtacggca tacagcacgc cgccaacgat
     1201 catcgcactt agcgcgcctt tggcgttggc gcgttcccag taaagaccca gcaccagcgg
     1261 ccacaggaaa acggcttcca gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc
     1321 tggcggcttc caggcggcaa gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga
     1381 catccgcttc agacgcgtct cgttttgcat ttgatccgga cggatattca gatagagatc
     1441 tttaatgatc gtagcggaac tttgcagcag ttgggcgtta attgtcgaca tgatcgcagc
     1501 cataggtgca gccagaaaga tcccggcagc aaaaggtggc agcactttta ccattaacgt
     1561 cgggatcacc agatccggta cggtgagatc ggggatcact gcacgaccta acgctccggc
     1621 cagatgcata ccgaacatca ggattgcgac tacaatcgta ccgatgatga tcccccggtg
     1681 tactgctttg ctgtctttat aagagataca gcgcaccgca gtatgcggca ggccaatcac
     1741 gccaaagcac accagtaccc agaacgacgt cataaaggca ggcgacagaa tatcgtcagc
     1801 gccttgtggc gtaaccagtt gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa
     1861 gccgccagcg gcatgtacca cgccaataag cagcacaacg gtgccaatca gcatcacaag
     1921 cccttgcatg gtgtcgttca gtacgctggc gcgaaagcca ccaaaggcgg tatataacgc
     1981 aatgctgata ccaaaaatca gcagcccggt ttcataagga atacccgccg cggtttccag
     2041 caggcgcgca ccgccgataa actgcacggt catcgcacca acgaacgcaa ccagcaaact
     2101 caaactcgcc agccacacca gaagacgact ctggtagcgg gcaaacagca tatcgttcag
     2161 cgtcattgca ttgtagcggc gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga
     2221 aagccagact gcaggaagct gaatcatcgc cagcaatacc cagcccagcc cgtatttata
     2281 agcagctcct ggcccgccga taaacgaact ggcactgata taggtcgcgg tgagcgtcat
     2341 cgccagcaca ataccgccca tagagcggct gccgaggaaa tactcattaa ggaaggtgcc
     2401 ggtgctccgt ttacgcatcg cataaaccga gataccgaac accaccacca gataggcgac
     2461 cagcggtaga attacttcaa gctgcatcgt catcctccag tgggatatcg cgatagataa
     2521 atttcaccat cgcccagcac agtccaataa acagcagcgg cgtcaggatg caggccatct
     2581 caaaccagcg cggaaagccg gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta
     2641 accataccgc cagatacaaa agggtcagcc ccagcgccca gcgcgcctct ttatgggcct
     2701 gaacaaaacg agtgtccatt ttttgtccct gatgggtgaa gaaagcgagg attgtacctt
     2761 atgggggttg tcgatcccca gtaataaaaa aggccggaaa atccggcctt ttgacgcttt
     2821 tgcagtctta tttttcctga agaccgagtt ttttctccag atagtggatg ttagtgccac
     2881 catgctggaa gttctcgtca ttcatgatgc ggatctgcag atcaacgttg gttttgatac
     2941 cgtcgatgat cagctcctgc agcgcattct tcatgcgggc aatcgccacg tcacggtttt
     3001 caccgtagca aatcagctta ccgatcattg agtcatagta cggcggtacg gtgtagcccg
     3061 cgtagatatg agactcccaa cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt
     3121 tgcctgggct tggcaggaag gtgttcggat cttcggcgtt gatacgacat tccaccgcat
     3181 ggccgcgaac gtgaacttct tcttgcttga tcgacagcgg ttgaccggca gcgatacgca
     3241 gctgttcttt gatcaggtca acgccggtga tcatttctgt aaccgggtgt tctacctgaa
     3301 tacgggtgtt catttcgatg aaatagaact cgccgttttc gaacaggaac tcgaaagtac
     3361 ctgcaccgcg atagccgata tcaacacacg ctttagcaca acgttcgccg atgtagcgac
     3421 gcagttccgg tgtaattccc ggtgctggtg cttcttcgac cactttctgg tgacgacgct
     3481 gcatagagca gtcacgttcc gccagataga tagcgttgcc ctgaccgtca gccagtacct
     3541 gaatctcgac gtggcgagga ttttccaggt atttctccat gtaaaccata tcgttattga
     3601 atgcggcttt cgcttccgca cgggtcatgg agatggattg tgccagttca gcgtcgccgc
     3661 gcactacacg cataccacga ccgccgccgc cgccggaggc tttgataatc accggataac
     3721 ctatgcgttt agcaatggca cggtttttat ccatatcgtc gcccagcggg ccgtcagaac
     3781 ctggtacgca aggaacgccc gcttttttca tcgccgcgat tgccgacact ttgtcaccca
     3841 tcaggcgaat agtgtctgct ttcgggccga tgaagataaa gcctgagcgt tcaacctgct
     3901 cggcaaagtt ggcgttctcg gagaggaagc cgtaacccgg atggattgct actgcgccgg
     3961 tgatttcagc ggcgctgatg attgccggga tgttcagata actttttact gacggagcag
     4021 ggccgataca gaccgtttca tctgccagta atacgtgttt tagatcgtga tccgcgctgg
     4081 agtgcacagc gacagtcttg atgcccagtt ctttacaggc acgaagaata cgcaatgcaa
     4141 tctcgccgcg gttggcaata acaattttat ccagcatgtt cgcctcgtta ctcgatgacg
     4201 accagcggct cgtcaaattc taccggttgt ccactttcga ccagaattgc tttcacggta
     4261 ccggatttgt ccgcttcgat ctggttcatc attttcatgg cttcaacgat gcacagggta
     4321 tcgcccacgt tgactttctg acccacttcg atgaacgctt ttgcgtccgg gcttggggtg
     4381 cggtagaaag taccaaccat cggggagcgt acgatgtgac cactgatttc cgctgctgct
     4441 ggcgcttcca tggaaggaac ggtcgccgga gcggctgcgt tagattgagc tggctgctgc
     4501 atcattggtg cagcgtaagc ttgttgcatc acagggaaac ttgcggcagg agctgcacgg
     4561 ctaatgcgta ctgactcttc gccttcagaa atttccagtt cggagatgcc tgattcttca
     4621 accagctcga tcagtttttt aatcttacga atatccatga gtgggttccg tactctttgt
     4681 ttagtgtgat tgtgacaggc gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt
     4741 atgcatagcg ataaattcca gcaggccggg acgccggtct attttgcccc agagtgtcaa
     4801 tgttagactt gacggacatt gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat
     4861 ataccttcgt tgcgccaatg tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg
     4921 aggtcgcaca ttataacgat ttcgtagcaa ttggcagcta aatactggtc ttatcaggga
     4981 agataatcaa cagctaacat gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc
     5041 acaattcacg aaattagcgc catcatcgac ggaacttctt ataacgtaag gtgaaaagct
     5101 gtaaagccag ccctgcgaag atgaggggct gcaatgagat aatcttcaca gaccacaaat
     5161 aatgtaggga tgccaggatc gcgacaaaat agacgacatt acgcagcggt tgctggggat
     5221 tacccaattt tcactgcatt gtctggatag atgtgaatgc taaagcacgc aaaattaacc
     5281 gattgacaat gctcagcatt aaataggttc gagtgattaa tttgtagtca aacaaaatca
     5341 gattattccc cccattttgc agaaggtaaa ctgttttgtc aggcggcagt gcggctatct
     5401 gatgctttca gaatttgcca cctgacaata aaaaagccga tttggtagca aaatcggctt
     5461 cggtgagttt gcagttttca tcacatactc cgacaagctt tttcaagcat gacggaggtt
     5521 gcgttaatgc aaggtttcta cccagggata aaagtgaccg ttacggcgga tgaatatctg
     5581 tgttgtcaga ggatggtcat cctcacggaa gcattgtttt gttagctgtc gtaatgcact
     5641 gagatttgct ggctgatcat tgtgggtgaa aaacagtttt cctctggagg gaacggtcac
     5701 taacaggtcg ccgcattcag gatcgggtga ttgcacccaa agatcgtcaa tgagcatcag
     5761 actgggctca taagtacccc ctgcggagat aacggcaact tttccatcgt ttgcgcctgt
     5821 atcaacgatt tcagccgtaa cttccggcaa tatttgcttc aaattactga tggcttgttc
     5881 gcggtcaatg gtcacttcgt agcgcatcag atagcgcatg gtttcgctac catcttcagc
     5941 aaacaagata attaactcat cgttaaagtc ttcccaggca aaatccagcg aggcgttgag
     6001 gacctctgga tcagactcat actggctaag ctgttggcga acggtatcca gccaggcttt
     6061 ttctttaatc accggaacaa tacagcgccc ggtcggcagc atttctgcaa gctgtttcag
     6121 catatctgca gccacttctt gcagcgggtc tggtgcggct tgataacgtt tgtaggcatc
     6181 atcaagggtg atttccaccg tttggtgacc cgtatcaatg cgcatcaatt tgtggtcagt
     6241 cgaaatcatg actttccagt ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac
     6301 gaaagctgtc cggctaaccg cttttcccgt taaccgattg aataatgatg tcaaaatgag
     6361 ttccctctct tattattcct tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg
     6421 gatctggtta ttaatgatgg cctcggcaaa gttcggtgcc tctgacagag atatctcttt
     6481 tgccgcctgg gtatagaatg attccggtaa atcggcgacc agtcgctgcc aggcttgtgc
     6541 gcggcgttct ggtggcgtca ttactgaatc caccccttgc aaacggacat tacgcagaat
     6601 aaatggcatg accgtggttg gcagagtaaa accacccgcc agaccacagg ccgccacgca
     6661 gccgccgtaa ttcatttgcg ccagcacttt tgccagcact ttgtcgccaa cagtgtcaat
     6721 tgccccagcc cagacttgtt tttccagagg acgggattcg gcaaactcat cacgagggag
     6781 aatacggctg gcacctaaac ttttcagata ttcatgggta ctttcgcgac cggaaacggc
     6841 aacgacctga taacccaact tatgcagcag cgccacggcg gtactgccga cgccaccact
     6901 ggcacccgtc acgacaatct ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat
     6961 cacacacagc atggcggtaa aaccggcagt accgataatc attgctttac gcgcgtccag
     7021 cccttgcggc atggcaacca gccagtcacc tttcactcgc gcctgttccg ccagcccacc
     7081 ccagtggttt tcaccaacgc cccagccagt gagtaacacc tcctgaccgg catgaaaacg
     7141 cggatcttcg ctggtgcgta cagttccggc aaaatcgatc ccaggaatca tcggaaaatt
     7201 acggatgatt tttcccttac cggtaatcgc cagcgcatct ttgtagttca ggctcgacca
     7261 gtgaacatca accgtgacat cgccctccgg caggcgactt tcgtccagag tctgtaccga
     7321 tgcgagagtt ttgccgtcct gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt
     7381 gcatgatgga ttggaaaatc atttctgaag actatactcg ctaatggaaa agcaaagccg
     7441 atgaagcgca ataaattgcg agataattct gatttgctag tatgcccgct tcctcactat
     7501 cggagttaac acaaggatga gattaacgac gaaattttcg gcctttgtta cgctgctcac
     7561 cgggttaaca atttttgtga ctttgctggg ctgttcgcta agtttctaca acgccattca
     7621 gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg attgataccc acctagtgtc
     7681 gaatgacttc agcacattaa ggccacaaat taccgaatta atgatgtcgg cagatatcgt
     7741 tcgtgtagac ctgctgcatg gtgataaaca ggtttatacc ctggccagaa atggtagtta
     7801 tcgtccggtt ggcaccagcg atctgtttcg tgaactgagc gttccgttga taaagcatcc
     7861 ggggatgtcg ctgcgtctgg tttatcagga tccgatgggc aactatttcc attcgttgat
     7921 gaccaccgcg ccgctcacgg gggcgattag ctttatcatt gttatgctct tcctggcggt
     7981 acgctggtta caacggcaac ttgccgggca agaattgctg gaaacccggg ctactcgtat
     8041 cttaaacggt gagcgtggct ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac
     8101 cagcagtgcg ctggatacgc tgcttcgtga aattcagaac gcacgcgaac aacacagccg
     8161 tcttgatacg ctgatccgct cttatgccgc ccaggacgtg aaaaccggcc tcaataaccg
     8221 actctttttc gataatcagt tagcaacgtt actggaagat caggagaaag taggtaccca
     8281 cgggatcgtg atgatgattc gtctgccgga tttcaatatg ttgagcgata cttgggggca
     8341 cagccaggtt gaagaacagt tcttcactct gacgaatctg ctgtcgacat ttatgatgcg
     8401 ctaccctggc gcactgctgg cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca
     8461 ccggacgtta aaagaggcag agagcatcgc cggtcagtta atcaaagccg tcgatacctt
     8521 gccgaacaat aaaatgctcg atcgcgacga tatgatccac attggtatct gcgcctggcg
     8581 tagtggtcag gataccgagc aggtaatgga acatgcagag tctgccacgc gtaatgcggg
     8641 attgcagggc ggcaatagct gggctattta cgatgactcg ttgcctgaaa aaggacgcgg
     8701 taatgttcgc tggcgttcgc ttatcgagca aatgctcagt cgcggcggcc cgcgccttta
     8761 tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat
     8821 cttcgatggt aatgaagagg ttagctcggc ggagtatatg ccgatggtct tgcagtttgg
     8881 cttatcggaa gagtatgacc gtctgcaaat cagccgtctt attccactat tgcgttactg
     8941 gccagaggaa aatctggcga ttcaggttac cgttgagtcg ctgattcgcc cgcgttttca
     9001 gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca caacgaaaac gcataattat
     9061 tgaacttgca gaggccgatg taggtcaaca tatcagtcgt ttacaacctg ttattcgttt
     9121 agtgaatgct ttaggggtac gggtagccgt caaccaggct ggtttgacgc tggtaagcac
     9181 cagttggatc aaagaactta atgttgagtt actcaagctc catccggggc tggtcagaaa
     9241 cattgagaag cgaacggaga accagctgct ggttcaaagc ctggtggaag cctgctccgg
     9301 gaccagcacc caagtttacg ccaccggtgt gcgttcgcga agcgagtggc agaccctgat
     9361 tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg tcctcacagc cacttgatac
     9421 taacgtgaaa aaatattcac aaagatactc ggtttaacct gccgtttaat ccgttttcac
     9481 gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt ctgctcgcca gattgttgca
     9541 gcacatatgc agatgaatga ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc
     9601 attaagcctt tctggactca ggcagagatt tgtaacaaag gaaacgaact gcactaattt
     9661 tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg
     9721 gattttcttt tccgccccag ctttcaggat tatcccttag tatgttgaaa aaatttcgtg
     9781 gcatgttttc caatgacttg tccattgacc tgggtactgc gaataccctc atttatgtaa
     9841 aaggacaagg catcgtattg aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg
     9901 gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc
     9961 cgggcaatat tgctgccatt cgcccaatga aagacggcgt tatcgccgac ttcttcgtga
    10021 ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag caacagcttt atgcgtccaa
    10081 gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc
    10141 gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg
    10201 ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg ttctatggtg gttgatatcg
    10261 gtggtggtac cactgaagtt gctgttatct ccttgaacgg cgtggtttac tcctcttccg
    10321 tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg
    10381 gttctctgat cggtgaagcc accgcagaac gtatcaagca cgaaatcggt tcggcttacc
    10441 cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa cctggcagaa ggtgttccac
    10501 gcggttttac cctgaactcc aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta
    10561 ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc ggaactggct tccgacatct
    10621 ccgagcgcgg catggtgctc accggtggtg gcgcactgct gcgtaacctt gaccgtttgt
    10681 taatggaaga aaccggcatt ccagtcgttg ttgctgaaga cccgctgacc tgtgtggcgc
    10741 gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag
    10801 agtaatcgga tgcaggcagg ggaaatgtct gtttaccctg cctggtctga tacgagaata
    10861 cgcataactt atgaagccaa tttttagccg tagcccgtcg ctacagattc gccttattct
    10921 ggcggtgctg gtggcgctcg gcattattat tgccgacagc cgcctgggga cgttcagtca
    10981 aatccgtact tatatggata ccgccgtcag tcctttctac tttgtttcca atgctcctcg
    11041 tgaattgctg gatggcgtat cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa
    11101 ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata
    11161 caaacaggag aatgcgcgtc tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca
    11221 gaaaatggtg actcaggtta tctccacggt taacgatcct tatagcgatc aagttgttat
    11281 cgataaaggt agcgttaatg gcgtttatga aggccagccg gtcatcagcg acaaaggtgt
    11341 tgttggtcag gtggtggccg tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc
    11401 gacccacgcg ctgccaatcc aggtgctgcg caacgatatc cgcgtaattg cagccggtaa
    11461 cggttgtacg gatgatttgc agcttgagca tctaccagcg aatacggata ttcgtgttgg
    11521 tgatgtgctg gtgacttccg gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt
    11581 tgtctcttcc gtaaaactcg atacccagcg cgcttatact gtgattcagg cgcgtccgac
    11641 tgcagggctg caacgtttgc gttatctgct gctgctgtgg ggggcagatc gtaacggcgc
    11701 taacccgatg acgccggaag aggtgcatcg tgttgctaat gaacgtctga tgcagatgat
    11761 gccgcaggta ttgccttcgc cagacgcgat ggggccaaag ttacctgaac cggcaacggg
    11821 gatcgctcag ccgactccgc agcaaccgac gacaggaaat gcagctactg cgcctgctgc
    11881 gccgacacag cctgctgcta accgctctcc acaaaggact acgccgccgc aaagtggtgc
    11941 tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga gctatcgtag ccagggacgt
    12001 tgggtaatct ggctctcttt cctcattgcg ctgttgctgc aaatcatgcc ctggccggat
    12061 aacctgattg ttttccggcc aaactgggtg ttactcatct tgttgtattg gatcctggcc
    12121 ttgcctcatc gcgtaaatgt gggcacaggt tttgtgatgg gtgccatact ggatctgatc
    12181 agcggctcga cgcttggcgt acgcgtattg gcgatgagca tcattgctta cctggtggcg
    12241 ctgaaatacc agcttttccg caacctcgca ttatggcagc aggcgctggt cgtcatgttg
    12301 ctttcgctgg tggtggatat tattgttttc tgggcagagt ttttagtgat taacgtctct
    12361 ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg tgctctggcc gtggattttc
    12421 ttgctgatgt gtaaagtccg tcagcagttt gcagtgcaat aaaggtttct atgacttctc
    12481 tgtatttagc ttccggttct ccgcgtcgtc aggagttact tgcgcaactt ggcgtgacct
    12541 ttgaacgtat tgttacgggc attgaggagc agcgtcagcc gcaggagagc gcgcagcagt
    12601 atgttgtgcg tctggcgcgc gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc
    12661 tcccggtgct gggtgcggat actatcgtta tcctgaatgg agaagtgctg gagaaaccgc
    12721 gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc gggtcagacc catcaggtga
    12781 tgacggcagt ggcgttggcc gacagccagc acattctcga ttgcctggtg gtcaccgatg
    12841 tgactttcag aacgttaaca gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt
    12901 tagataaagc aggtgcatac ggtattcagg ggctgggtgg ctgttttgtc aggaagataa
    12961 atggcagcta tcacgccgta gtcggcttac cgctggttga aacgtatgaa ttattaagta
    13021 attttaacgc actgcgtgag aaaagggata aacatgacgg ctgaattgtt agtaaacgta
    13081 acgccttcgg aaacgcgagt ggcgtatatt gatggcggta ttctgcagga aattcatatt
    13141 gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca agggtcgtat aagtcgtgta
    13201 cttccgggta tgcaggcggc ttttgtagat attgggctgg ataaagccgc gtttcttcat
    13261 gcatccgaca tcatgccgca caccgaatgt gtggcgggtg aagaacaaaa gcaattcacg
    13321 gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa
    13381 gatccgcttg gcactaaagg tgcgcgcctg accaccgata tcacgctgcc ttctcgctat
    13441 ctggtgttta tgccaggggc ttctcacgtt ggggtttccc aacgtattga aagcgaatca
    13501 gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg acgagcaggg cgggtttatc
    13561 atccgtaccg cagcggaagg ggttggcgag gctgaactgg cctccgatgc cgcttatctg
    13621 aaacgcgtct ggaccaaagt tatggagcgt aaaaaacgcc cgcagacccg ttatcagctg
    13681 tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt tcgccgacgc cgaactggac
    13741 cgcattcgcg ttgactcacg cctgacttac gaagcattgc tggagtttac ctcggagtac
    13801 attcccgaga tgaccagcaa gctggagcat tacaccggac gccagccgat tttcgatctc
    13861 tttgatgtcg aaaacgaaat ccagcgagcg ctggaacgca aagtagaact gaaatccggt
    13921 ggttatctga ttatcgacca gaccgaagcg atgaccaccg tggacatcaa taccggagcg
    13981 tttgtcggtc atcgcaatct ggacgacacc attttcaata ccaatattga agcgacgcag
    14041 gctatcgctc gccagttacg gttgcgtaat ctgggtggga ttatcattat tgatttcatc
    14101 gatatgaata atgaagatca ccgccgccgc gtgctgcact cgctggagca ggcgttgagc
    14161 aaagaccggg tgaaaaccag cgttaatggt ttttcggcgc tggggctggt ggagatgacg
    14221 cgtaaacgca cccgcgaaag cattgagcac gtactgtgta acgaatgccc aacctgccac
    14281 ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt
    14341 cgtgtccacc atgcttacga ctccgaccgt ttcctggtct atgcttctcc ggcagtagct
    14401 gaagccttga aaggcgaaga gtcacactcg ctggcggaag tggaaatttt cgttggcaaa
    14461 caggttaaag tacaaattga accgctctat aaccaggagc agtttgacgt cgtaatgatg
    14521 taaacagatg ctgggccgcc atccggcaaa gggtttttga gtcacatttt tagcagacaa
    14581 ggagtgacgg gtgaggcgat tgccggggat tttactgctt actggagccg cgctcgttgt
    14641 gatcgctgcc ctgctggtta gcggcctgcg tattgcttta ccgcatcttg acgcctggcg
    14701 tccggaaatc ctcaacaaaa tagaatccgc gactggcatg ccggtagaag ccagtcagct
    14761 ctcagccagc tggcagaatt ttggcccgac gcttgaagca cacgacatcc gtgcagaact
    14821 aaaagatggc ggcgaatttt cggttaaacg cgttactctg gcgctggatg tctggcagag
    14881 cctgttacat atgcgctggc agtttcgcga cctcactttc tggcagctgc gctttcgcac
    14941 caacactcct atcaccagcg gtggtagcga tgacagtctg gaagccagtc acatcagcga
    15001 tctgtttctt cgtcaatttg accatttcga tctccgcgac agtgaagtca gttttctgac
    15061 gccatccggt cagcgcgccg agctggcgat cccacaactc acctggctga acgatccacg
    15121 tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt accggacagc acggcgtgat
    15181 gcaggtgcgc atggatttgc gcgatgatga ggggttgtta agcaatggtc gcgtctggct
    15241 acaggcggat gacatcgacc tgaagccgtg gctcggtaaa tggatgcagg acaatattgc
    15301 tctggaaacg gcacagttct cccttgaagg ctggatgacg atcgacaaag gcgatgtaac
    15361 cggcggtgac gtctggctga aacagggcgg tgccagctgg ttgggcgaga agcaaacgca
    15421 tacgctgtcg gtggataatc tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt
    15481 ctctattccc gatacacgga tcacgatgga cggcaaaccc tggccgagcg gagcattgac
    15541 gctggcctgg ataccggaac aggacgttgg cggcaaagac aataaacgca gtgacgaact
    15601 ccggattcgc gccagtaatc tggagctggc aggcctggag ggcgtacgcc cgttggccgc
    15661 gaaactttca cctgcactgg gtgatgtttg gcgctccaca caaccgagcg gcaagattaa
    15721 cactctggcg ctggatatcc cgcttcaggc ggcagacaag acccgttttc aggcatcgtg
    15781 gagcgatctg gcctggaagc aatggaaatt attaccgggt gcggaacact tctccgggac
    15841 gctttccggc agcgttgaaa atggtttgct taccgcgtcg atgaagcagg caaagatgcc
    15901 ttacgaaacg gtattccgtg cgccactgga aatcgccgac ggccaggcaa ctataagctg
    15961 gctgaacaat gacaaaggtt tccagctgga tgggcgtaat attgacgtta aagccaaagc
    16021 cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct aacgatgaac cctggctggg
    16081 tattctggct ggcatcagta ccgatgatgg ttcacaagcc tggcgctatt tcccggaaaa
    16141 cttgatgggt aaagacctgg ttgattattt aagtggtgcg attcagggcg gtgaagcgga
    16201 taacgcgacg ctggtttatg gtggcaatcc gcaactcttc ccctataaac acaacgaagg
    16261 tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt gccttccagc cggactggcc
    16321 tgcattaact aaccttgata ttgaactgga ctttattaac gacggtttat ggatgaaaac
    16381 cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc accgcagtga tccctgacta
    16441 ctcaaaagaa aaactgctga ttgacgctga cattaaaggt ccgggtaaag ccgttggccc
    16501 ttactttgat gagacaccgc tgaaagattc tctgggtgcg accctgcaag aactccagct
    16561 cgacggcgat gtgaatgctc gcttacatct tgatatcccg ctgaacggcg aactggtaac
    16621 cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt atcaaaccac tcgacagcac
    16681 cctgaaaaat ttgagcggta aattcagctt tatcaatggc gatctgcaaa gtgaaccact
    16741 gacagcaagc tggtttaatc agccgttgaa cgtggatttt tccaccaaag aaggggcaaa
    16801 agcctaccag gtagcggtaa acctcaacgg taactggcaa ccggcgaaaa ccggcgttct
    16861 gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg tgggatggta aagtgggcat
    16921 tgagctgcct tatcatgctg gcgcgaccta taacgtagag ctgaacggcg atctgaataa
    16981 tgtgagcagt cacttacctt caccgttagc caaacctgcg ggtgaaccac tagcggtaaa
    17041 cgttaaggtt gatggtaatc tcaacagctt tgagttaacc ggacaggctg gtgcggataa
    17101 ccatttcaat agccgctggt tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc
    17161 ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt ggcgttgaac tcaatatgcc
    17221 gccgatgaat ggtgccgagt ggctggcact gttccagaaa ggcgctgcgg agagtgtcgg
    17281 tggtgcagcg agtttcccac aacacataac gttacgtacg cctatgttgt cactgggaaa
    17341 tcagcaatgg aataacctga gtattgtttc gcaaccgacg gcaaatggca ccctggttga
    17401 ggcgcagggg cgtgaaatca atgccacgct ggcgatgcgt aataacgcgc cgtggctggc
    17461 gaatatcaaa tatctttatt acaacccgag cgtggcgaaa actcgtggtg attcaacgcc
    17521 gtcatcacct ttcccgacaa cggagcgcat taacttccgt ggctggccgg acgcacaaat
    17581 acgatgcgca gagtgctggt tctgggggca aaaattcggt cgcattgaca gtgatctcac
    17641 catttctggc gatacgttaa cgctgaccaa tggactgatt gatactggtt tctcgcggct
    17701 tactgccgat ggtgaatggg ttaataatct ggggaatgaa cgtacctcgc tgaaaggaaa
    17761 actgcgcggg cagaaaattg atgccgccgc agaatttttt ggtgtcacga cgccaatacg
    17821 ccagtcgtca tttaatgtgg attacgattt acactggcgc aaagcaccct ggcagccaga
    17881 tgaagcgacg ttgaatggca tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat
    17941 caataccgga catgccgggc aattgctgcg cttattgagc gtagatgccc tgatgcgtaa
    18001 gctgcgtttt gatttcagag acacttttgg cgaagggttc tattttgact ccattcgcag
    18061 caccgcgtgg attaaagacg gcgttatgca caccgacgac acgctggtgg atggcctgga
    18121 agcggacatc gctatgaaag ggtcggtaaa tctggtacgt cgcgacctga atatggaagc
    18181 ggttgttgca ccagagattt ctgcgacggt gggcgtggct gcggcttttg cggttaaccc
    18241 cattgttggc gcggcagtgt ttgccgccag taaagtgctg gggccgctgt ggagcaaagt
    18301 ctccattttg cgctatcaca tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt
    18361 gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc
    18421 tcatatgata atcgttgcca aaggccaacg agccagaaca taaccgtagg tcggataggg
    18481 cgttcacgcc gcatccggca gccgtaaaaa atcctctact gcagtaacta acgagtagca
    18541 aaaacgatga gtcttaacct ggtaagtgaa caattgctag cggcgaacgg cctgaaacat
    18601 caggacttgt tcgcgatcct cggtcaactg gccgaacgtc gccttgatta tggcgatctc
    18661 tattttcagt cgagctatca cgaatcctgg gttttagaag accgcattat taaagatggt
    18721 tcttacaaca tcgatcaggg cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt
    18781 gcttacgctg accaaatcag cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc
    18841 atcgtccgtg atagtggtga tggcaaagta cagacgctgg gcgcggtaga gcatagcccg
    18901 ttgtatacct cggtagatcc gctgcaaagc atgagccgtg aagagaagct ggatatcctg
    18961 cgtcgcgtcg ataaggttgc tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc
    19021 ctcagtggtg tctatgaatt aattctggtt gcggccaccg acggcacgct agcggcggat
    19081 gtccgtccgc tggtgcgtct ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa
    19141 cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt tcttccttgc cgatcttgac
    19201 ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt
    19261 tctgccgttg ctgcaccagc gggcaccatg ccggtagtac ttggcgcagg ttggccgggc
    19321 gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg acttcaaccg ccgcggcact
    19381 tcagtattta gtgggcaggt cggggagctg gtggcttcag aactgtgtac cgtggttgat
    19441 gacggcacga tggtcgatcg ccgtggttcg gtggcgattg atgacgaagg tacgccaggc
    19501 cagtacaacg tgctgattga gaacggcatt ctgaaaggct acatgcagga taaactcaac
    19561 gcgcgtttga tggggatgac gccgactggc aacggtcgcc gtgaatccta cgcccatctg
    19621 cccatgccgc gtatgaccaa cacctatatg ctgccgggta aatcgacccc gcaagaaatt
    19681 attgaatccg ttgagtacgg tatctatgca ccgaactttg gtggcggtca ggtggatatc
    19741 acctccggca aattcgtttt ctccacttca gaagcatatc tgattgaaaa cggtaaagta
    19801 acgaagccgg tgaaaggcgc aacgttgatt ggttccggta tcgaaaccat gcagcagatt
    19861 tcgatggttg gcaacgacct gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg
    19921 caaagtttgc cggttggcgt gggccagcca acgttgaaag tcgataacct gactgttggc
    19981 ggtactgcgt aataatttca ttattttcag aaggataatt aatctttcta cgtgcacgaa
    20041 cggtcccctc gctcctctgg ggttagggtg aggggaaccc gttggcacag gtttgtacaa
    20101 cgtaacagta caatatgaat tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt
    20161 taccgacctc aacaaaataa tccgtcagcg agttgaccac gacctgtacc ttcagcggca
    20221 gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg atctgactga taacgcggca
    20281 gcaggatctc cagctcccca cgattgatct cgttgatcac ccacatcagc ggcacgtagg
    20341 cgatcccggc acccgccgtt agccagcgca ccagcgtcat cggatcatta gtcacaaatc
    20401 tcccctgcgg gatcaggcga gtcgagatcc cttccggtgc gatcagttca aattcattgt
    20461 cgggccgcac gctgtattca agccatgaat ggctacttaa atcggcgggt ttttccggta
    20521 tgccgtattg tgtgagatag cttttcgcgg cgcacaccac catcggcatc gcgccaagac
    20581 ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt
    20641 cggcaatcag gtcgggggct ggaattccgg taaccagatt gacgctcaaa cctgggtatt
    20701 ctttcagcat ttttgctgtc agcccggcga gaacattttg tgccatagtt gaagaacagc
    20761 caatgcgcag cgtcccgatg ggggtgttat tgaaggcata cagttgctca tgaacatcct
    20821 gcacttcatg aagcatccga cggcagccct ggtagtaaat tctgccggct tcggtcaggc
    20881 caatgctgcg tgtgctacgg tttaacagct ttacctgcaa ctcatcttcc agttttgaca
    20941 ccgtctgact gatggacgaa acgctcatct gtagctgtct ggcggcggcg gtaaaagagc
    21001 caaattcaac tactttggca aacaccgaca tgcgttttag tcgttccatt attcactctg
    21061 acttaaaagt gatttagatc acataatata gataacagca taacagttac gctaatatat
    21121 taaatatcaa tctacagcaa tgttgctctc gcccggcttt ccatgccctt ctctgcggcg
    21181 acagatgctg aaaataataa cgcctgctct ctctttacaa ccaaggtcaa catgagtctg
    21241 tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga tattttttga attgctttta
    21301 tcactggcga ttttctggct ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt
    21361 gtctggcatc cggcgttgtt caacaccgcg ctctattgct gcttgtttta tttgatatcg
    21421 cgactgttcg tttgaggttg aagtgaaaac actaataaga aaattctccc gtacggccat
    21481 cacggtcgta ttagtcattc tggccttcat cgcaattttt aatgcctggg tctattacac
    21541 cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga
    21601 cgtttctgga ctcattaccc aggtgaatgt tcatgataac cagctggtga aaaaaggaca
    21661 gatactgttc accatcgacc agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga
    21721 tgttgcttat tatcaggtac tggcacagga gaaacgccag gaggccggac gtcgtaaccg
    21781 tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag gctaacaacg tactacaaac
    21841 ggttctgcat cagttagcga aagcgcaggc gacccgcgat ctggcaaaac tggatcttga
    21901 acgcacggtg atccgcgcgc cagcagatgg ctgggtgacc aacctcaacg tctataccgg
    21961 tgagtttatt actcgaggat caacggcggt tgcgctggtg aaacagaact ccttctatgt
    22021 actggcctat atggaagaaa ctaagctgga aggggtgcgc cctgggtatc gtgcagagat
    22081 cacgccgctt ggcagtaaca aagtgctgaa agggactgtt gatagtgttg ccgcaggggt
    22141 caccaacgcc agcagcacgc gtgacgacaa agggatggcg actatagact ctaaccttga
    22201 atgggtgcgt cttgcgcaac gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa
    22261 catctggcct gcgggcacca ctgctacagt ggtggtcact ggcaaacaag atcgcgacga
    22321 aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg
    22381 atgggtattt tctccattgc taatcaacat atacgctttg cggtaaaact ggcgaccgcc
    22441 attgtactgg cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta
    22501 ctgacagcgg cgattgttgc cgccggtccg gcctttgctg cgggaggtga accgtattct
    22561 ggcgctattc gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc
    22621 ggactggtga tcatcattgc gatgatccgc gcaccattat tgatgattct ggtgtgctgt
    22681 atctgggccg gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtacgcg
    22741 tgggggctgg ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg
    22801 cttacgccgc agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg
    22861 attatggcgg atttgctctt ttctccgcga tcgatcaaac aagaagtgga tcgagagctg
    22921 gaaagtttgc tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt
    22981 gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca ccacggcact acaaggcatg
    23041 cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg
    23101 atcaatacgc tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat
    23161 acgcgcccgg aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc
    23221 gcgcaggacg tccacaagca gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa
    23281 cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc
    23341 aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc
    23401 gaaccggaag tcaaagtaga gtcagccgaa cgtcatcatg cgatggttaa cttctggcga
    23461 accacacttt cctgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc
    23521 agtggtgcaa tggtgatgat tgcggtagtg acgtcactgg caatgcgttt gccgaatcca
    23581 cgcatggtgg cgatcgactt tatctacggg acgctggccg cgctgccgtt agggctgctc
    23641 tactttttgg tgattatccc taatacccaa cagagcatgt tgctgctgtg tattagcctg
    23701 gcagtgctgg gatttttcct cggtatagaa gtacagaaac ggcgactggg ctcgatgggg
    23761 gcactggcca gcaccataaa tattatcgtg ctggataacc cgatgacttt ccatttcagt
    23821 cagtttctcg acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt
    23881 ttgctggtgc gggataaatc gcgcgacagg actggacgtg tactgcttaa tcagtttgtt
    23941 tctgccgctg tttccgccat gactaccaat gtggcacgtc gtaaagagaa ccacctcccg
    24001 gcactttatc agcagctgtt tttgctgatg aataagttcc caggggattt gccgaaattt
    24061 cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt
    24121 aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct
    24181 gcccgtagcg atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc
    24241 taccaggaaa agctacgcat ctggcaagcg ccaccgcagg tgacggaacc ggtacatcgg
    24301 ctggcgggga tgctccataa gtatcaacat gcgttgaccg atagttaagt caaagccgac
    24361 gccaaaagcg tcggtttttt catggctata cttagcgatg aacggcagaa ctcgccgcga
    24421 aacgtgacgg tggcaacaga tgaatattta tacctttgat tttgatgaga ttgagagtca
    24481 ggaggatttt tatcgtgact ttagccaaac ctttggtctg gcgaaagata aggtacgcga
    24541 tctcgactca ctatgggatg tgttaatgaa cgatgtcctg ccgctaccac ttgagattga
    24601 atttgttcat ctgggagaga aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga
    24661 tgaggcagag gaagagctgg aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa
    24721 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    24781 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    24841 gcctttctct tccgcttttt tgttcagcat ttcacgtata tccattggcg aagacgccac
    24901 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga
    24961 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    25021 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgacc
    25081 catatcacaa aatcaagcat acactaatta ttgaactaat taataccacg ctaactgttt
    25141 tagttgatac taagtgttaa aaatgtgaat tttaata
//
LOCUS       DAJSKJ010000044        24987 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000044 DAJSKJ010000000
VERSION     DAJSKJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.044"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            269..1684
                     /gene="gltX"
                     /locus_tag="ON345_001527"
     CDS             269..1684
                     /gene="gltX"
                     /locus_tag="ON345_001527"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR3071611.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            complement(1735..2127)
                     /locus_tag="ON345_001528"
     CDS             complement(1735..2127)
                     /locus_tag="ON345_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR3071612.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2129..2488)
                     /locus_tag="ON345_001529"
     CDS             complement(2129..2488)
                     /locus_tag="ON345_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR3071613.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            2709..2784
                     /locus_tag="ON345_001530"
     tRNA            2709..2784
                     /locus_tag="ON345_001530"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2742..2744,aa:Ala,seq:ggc)
     gene            2824..2899
                     /locus_tag="ON345_001531"
     tRNA            2824..2899
                     /locus_tag="ON345_001531"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2857..2859,aa:Ala,seq:ggc)
     gene            3108..5297
                     /locus_tag="ON345_001532"
     CDS             3108..5297
                     /locus_tag="ON345_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR3071614.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5348..6550)
                     /gene="nupC"
                     /locus_tag="ON345_001533"
     CDS             complement(5348..6550)
                     /gene="nupC"
                     /locus_tag="ON345_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR3071615.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            6886..8124
                     /locus_tag="ON345_001534"
     CDS             6886..8124
                     /locus_tag="ON345_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR3071616.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8265..8591)
                     /locus_tag="ON345_001535"
     CDS             complement(8265..8591)
                     /locus_tag="ON345_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR3071617.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8706..9962)
                     /locus_tag="ON345_001536"
     CDS             complement(8706..9962)
                     /locus_tag="ON345_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR3071618.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLTALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10166..11131
                     /gene="glk"
                     /locus_tag="ON345_001537"
     CDS             10166..11131
                     /gene="glk"
                     /locus_tag="ON345_001537"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR3071619.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11350..11676
                     /locus_tag="ON345_001538"
     CDS             11350..11676
                     /locus_tag="ON345_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR3071620.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            11698..12945
                     /locus_tag="ON345_001539"
     CDS             11698..12945
                     /locus_tag="ON345_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR3071621.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            12960..14045
                     /gene="ypdF"
                     /locus_tag="ON345_001540"
     CDS             12960..14045
                     /gene="ypdF"
                     /locus_tag="ON345_001540"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3071622.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            14045..15082
                     /gene="ypdE"
                     /locus_tag="ON345_001541"
     CDS             14045..15082
                     /gene="ypdE"
                     /locus_tag="ON345_001541"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3071623.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAACQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15107..16189
                     /locus_tag="ON345_001542"
     CDS             15107..16189
                     /locus_tag="ON345_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR3071624.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQK"
     gene            16211..17602
                     /gene="ptsP"
                     /locus_tag="ON345_001543"
     CDS             16211..17602
                     /gene="ptsP"
                     /locus_tag="ON345_001543"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3071625.1"
                     /translation="MAYSRDNKRIDIAANIGTALEAPGAFANGAEGVGLFRTEMLYMD
                     RDSAPDEQEQFEAYQQVLLAAGDKPIIFRIMDIGGDKSIPYLNIPQEENPFLGYRAVR
                     IYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLR
                     HAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPI
                     TPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKS
                     QLRQLDSEACRELARQACECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDFSNKE
                     QAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISI
                     ARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDA
                     QSILTLLETELTF"
     gene            complement(17605..18462)
                     /locus_tag="ON345_001544"
     CDS             complement(17605..18462)
                     /locus_tag="ON345_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3071626.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18475..19209)
                     /gene="ypdB"
                     /locus_tag="ON345_001545"
     CDS             complement(18475..19209)
                     /gene="ypdB"
                     /locus_tag="ON345_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR3071627.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19224..20921)
                     /gene="ypdA"
                     /locus_tag="ON345_001546"
     CDS             complement(19224..20921)
                     /gene="ypdA"
                     /locus_tag="ON345_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR3071628.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21298..22536
                     /gene="alaC"
                     /locus_tag="ON345_001547"
     CDS             21298..22536
                     /gene="alaC"
                     /locus_tag="ON345_001547"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR3071629.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(22601..22672)
                     /gene="ypdK"
                     /locus_tag="ON345_001548"
     CDS             complement(22601..22672)
                     /gene="ypdK"
                     /locus_tag="ON345_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR3071630.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(23028..23948)
                     /gene="lpxP"
                     /locus_tag="ON345_001549"
     CDS             complement(23028..23948)
                     /gene="lpxP"
                     /locus_tag="ON345_001549"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR3071631.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEHEKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            24301..24543
                     /locus_tag="ON345_001550"
     CDS             24301..24543
                     /locus_tag="ON345_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR3071632.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(24620..24895)
                     /gene="ypdI"
                     /locus_tag="ON345_001551"
     CDS             complement(24620..24895)
                     /gene="ypdI"
                     /locus_tag="ON345_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCR3071633.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
BASE COUNT         6006 a         6140 c         6596 g         6245 t
ORIGIN      
        1 ctaatcaccc ccgctgtgtg gagtcgcatt atagggagag ttcaaaatga gtcaacgcat
       61 tttctaaaga aattgttcgt tcgtcgtaaa tttaagcaag atgatcgcaa aacagaccgt
      121 gttgcgcaat ttgtcaacga aaacaataat gcgtaaggta gaaacccgaa ctacattgag
      181 gaatcaggcg ggagtgatag aatatcgccc actcaatttt tccaggattt gccggttgtc
      241 ggcatctttc taaacgtaag gccatttcat gaaaatcaaa actcgcttcg cgccaagccc
      301 aacaggctat ctgcacgttg gcggcgcgcg tactgctctt tactcctggc tttttgcacg
      361 taaccacggc ggtgagttcg tgctgcgtat tgaagacacc gatcttgagc gttccacgcc
      421 ggaagctatc gaagccatta tggatggcat gaactggctg agcctggagt gggatgaagg
      481 tccgtactac cagaccaaac gttttgatcg ctacaacgcg gtgatcgatc agatgctgga
      541 agagggcact gcttataaat gctattgctc taaagagcgc ctggaagcgc tgcgcgaaga
      601 gcaaatggcg aaaggtgaga agccgcgtta tgacggtcgc tgccgccaca gccatgagca
      661 tcacgctgat gatgaaccgt gcgttgtacg ttttgctaac ccgcaggaag gttctgttgt
      721 ttttgacgat cagatccgtg gtccgatcga gttcagcaac caggaactgg acgatcttat
      781 tatccgccgt accgatggtt ccccaaccta taacttctgt gtggttgtcg atgactggga
      841 tatggaaatc acccacgtta tccgtggcga agaccatatc aacaacacgc cacgccagat
      901 caacattctt aaggccctga aagcgccggt gccggtttac gcgcacgttt ctatgatcaa
      961 cggcgatgac ggtaaaaaac tgtccaaacg tcacggggcg gtcagcgtaa tgcagtatcg
     1021 tgatgacggt tatttgccag aagcactgct gaactatctg gtgcgtctgg gctggtccca
     1081 cggcgatcag gaaatcttca ctcgtgaaga gatgatcaaa tacttcaccc tgaatgccgt
     1141 cagcaaatct gccagtgcgt tcaacaccga caagctgctg tggctgaacc atcactacat
     1201 taacgcgctg ccgccggagt atgttgctac tcacttacag tggcacattg agcaggaaaa
     1261 tatcgatacc cgtaacggcc cgcagctggc tgatctggtg aaactgctgg gcgaacgctg
     1321 caagacgctg aaagagatgg cacagagctg ccgttatttc tacgaagatt ttgctgagtt
     1381 cgatgccgac gccgcgaaaa aacatctgcg tccggtagcg cgtcagccgc tggaagtggt
     1441 tcgtgacaaa ctggccgcga ttactgactg gaccgctgaa aacgttcatc acgctattca
     1501 ggcgacggcg gatgagctgg aagtgggtat gggtaaagtt ggtatgccgc tgcgtgtcgc
     1561 cgtaaccggt gcggggcagt ctccggcact ggatgttacc gttcacgcaa ttggtaggac
     1621 ccgcagtatc gagcgtatca acaaagcgct ggcttttatt gctgaacgcg aaaaccagca
     1681 gtaattagcg cataaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc
     1741 tgattcgtca atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg
     1801 cgcgactttt tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata
     1861 cgccggggcg cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat
     1921 tggtgtgtta tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag
     1981 aataagtctg gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc
     2041 tctgatccaa cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt
     2101 ggtcatttta ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta
     2161 acaatccgat aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg
     2221 tcatctcttt cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag
     2281 gcgcttctcc ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat
     2341 tgacgtgaac cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc
     2401 cttccttgcg tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt
     2461 catctggtgt catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag
     2521 tagttcattt tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga
     2581 gcggttttcc aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa
     2641 tttgccgttg acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt
     2701 tacgtgacgg ggctatagct cagctgggag agcgcttgca tggcatgcaa gaggtcagcg
     2761 gttcgatccc gcttagctcc accaaatttt gcacccagca aatttggtac gtaaacgcat
     2821 cgtggggcta tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcggttcg
     2881 atcccgctta gctccaccaa atttccaacc ctcgctgcaa agcgggggtt ttttgtctct
     2941 gctttttgcc gcttttgtaa tacagtctac gtccgggtta gtgccgcctg gtgaaagcat
     3001 cattggatga aaaatcggca acaggctggc cccctgtttg cttcgcgatg cgaataaact
     3061 tattatttgt gtgcctgaaa accccgatca gtgagagtag tgtactcatg tttgtggagc
     3121 ataacctgat aaaaaatatc aagatattca cactagcgtt tacgctcacc gtggtactta
     3181 ttcagctatc ccgttttatt tcgccacttg ccattatcca ttccagttat atctttctgg
     3241 cgtggatgcc actgtgcgta atgctgtcaa tcttgtttat ctttggctgg cgcggtgtcg
     3301 ttcccgtttt atgcgggatg ttttgcacca atctgtggaa ctttcatctc tcttttttac
     3361 agactgcggt catgctcggt agccagacgt ttgtcgtgtt gtgtgcctgc gcaatattac
     3421 gctggcagct ggggacgcgt tggcgttatg gattgaccag ccgatatgtc tggcaacgtc
     3481 tgttctggct tggtttggtg acgccgatcg gcatcaaatg cagcatgtat cttgtgggga
     3541 gcttctttga tttcccgcta aagatatcca cctttttcgg cgatgcggat gccattttca
     3601 cggtcgttga tttgctaagc cttttcaccg ctgtgctgat ttacaacatg cttttctact
     3661 atctcactcg catgattgta agtccccact ttgcgcagat attgtggcgc agggatatcg
     3721 cgccgtcgtt gggcaaagag aaacgcgcat ttaccttaag ctggctggca gcgttaagcg
     3781 tgcttctact tctgttgtgc acaccttatg aaaacgactt tattgccggt tacctggtac
     3841 ccgttttctt catcatcttt accctcgggg tcggtaagct tcgctatccg tttttaaatc
     3901 tcacctgggc tgtttcaacg ctttgccttc tgaattacaa ccagaacttt ttgcaagggg
     3961 tggaaaccga atattcgctg gcatttattc ttgcggtgct gatttccttt agcgtttgcc
     4021 tgctctatat ggtgcgcatt tatcatcgca gtgaatggct taatcgccgc tggcatttgc
     4081 aggcgctgac agatccgtta acgctcctac ccaactttcg tgcgttggaa caagcgccgg
     4141 agcaagaggc gggcaagagt ttttgctgcc tgcgcattga taatcttgag tttatgagtc
     4201 gtcattacgg cttaatgatg cgcgttcact gtatccgctc aatttgccgt acgctgctgc
     4261 cgttgatgca ggaaaacgaa aagttgtatc aattgccggg tagtgagctg ctgttagtgc
     4321 tgagcgggcc ggaaacggaa gggcgactcc agcatatggt taacatcctg aatagtcggc
     4381 aaattcactg gaacaatacc gggctggata tgggctatgg tgctgcctgg gggcgttttg
     4441 atggaaatca ggaaaccctg caacccttgt tggggcagtt aagctggctg gcggagcaat
     4501 cctgcgcaca tcatcatgtg ctggcgctgg atagcagaga ggagatggtt tccgggcaga
     4561 ccactaaaca ggtgctattg ctgaatacca ttcgcacggc gttagatcag ggtgatttgc
     4621 tgctctacgc ccagccaatt cgcaacaaag agggtgaagg ttatgatgag atcctcgcgc
     4681 gactgaaata tgacggcggc attatgaccc cggataagtt tctgcccctt attgctcagt
     4741 ttaaccttag cgcgcgtttt gatttgcaag tgctggaatc cttgttgaag tggctggcaa
     4801 cacacccttg cgacaaaaaa ggaccgcgct tttcagtcaa tttaatgccg ctcacgctgc
     4861 tgcaaaagaa tattgccggg cggattattc gtctgtttaa gcgttatcac atctccccgc
     4921 aggcggtcat tcttgagatc accgaggagc aggcgttttc taacgcagaa agcagcatgt
     4981 acaacatcga gcagctgcat aagtttggtt tccggattgc gattgatgac tttggcaccg
     5041 gatatgccaa ctacgaacgg ttaaagcgtt tgcaggctga tatcatcaaa attgatggcg
     5101 tctttgtgaa agatattgtc acgaacacgc tggatgcgat gattgtgaga tcaattaccg
     5161 atctggcgaa agcgaagtca ttgagtgtgg tcgcggagtt tgtcgagacg caacagcagc
     5221 aggcgctatt gcataagctc ggggtgcaat atctgcaagg gtatttgatt ggtcgcccgc
     5281 agccattagc tgattaacag gcgtaaaaaa accggggaat tatccccggc ttttttatgt
     5341 atggtgctta cagcaccagt gctgcgattg acgcagacag cacactcacc agggtagagc
     5401 cgtaaaccag cttcagaccg aagcgagaaa ccacgttacc ttgctcttca ttcaggcctt
     5461 taactgcacc tgcgataatc ccgattgaag agaagttagc gaaggaaacc aggaacacag
     5521 agatgatgcc ttcagcacgc ggagagagcg tggaagcaat tttctgcaga tccatcatcg
     5581 caacgaactc gttggaaacc agtttggtcg ccatgatact gcccacttgt agtgcttcac
     5641 tggaaggaac acccatcacc catgcaatcg gatagaagat gtagcccagg atgccctgga
     5701 aggagatgct gtagccaaac cagccagtaa cggtggcaaa cagtgcgttc agcgcggcga
     5761 tcagggcgat aaagccgatc agcatcgcgg caacgataat ggcaactttg aaacctgcca
     5821 gaatgtattc acccagcatt tcgaagaagc tctgaccttc gtgcaggttg gacatctgga
     5881 tgttttcttc actggcatca acacggtaag gattgatcag cgacagcacg ataaaggtgc
     5941 tgaacatgtt cagtaccagc gcagcaacga cgtatttcgg ttccagcatg gtcatgtatg
     6001 caccaacgat ggacatcgac acggtggaca ttgccgtggc agccatggtg tacatacgat
     6061 tacgggagat tttgccgagg atatctttat aggcaataaa gttttcagac tgacccagaa
     6121 tcagggagct gacggcgtta aaggattcca gtttgcccat gccgttgact ttggagagca
     6181 ggaaaccaat tgcgcggatg atcaccggca acacgcgaat gtgctggaga ataccgatca
     6241 gtgcagagat aaagacgatt gggcacagca ctttcaggaa gaagaatgcc aggccttgat
     6301 cattcatgct accaaagacg aagttagtcc cttcgttggc aaatccgagc agtttttcga
     6361 acatttcgga gaagcctttc acgaagccta aaccaacgtc ggagttcagg aagaaccacg
     6421 ccagtaacac ttcgataaca agcagttgaa taacataacg gatacgaatt tttttgcggt
     6481 cgctgcttac cagcagtgcg agaatcgcaa caacggcaag tgccagtaca aaatgaagga
     6541 cgcggtccat atttgctcca aatatgaggc aggttaaatt tccgtgcaca ttctatgtaa
     6601 catggttaag gaaaacgaga tctgacacac actataatga cgtcctgtga caggcttcaa
     6661 aaatagtgat ctgtcataca tttctgctat gttgtgtatg gaagctgaaa gttatgtaaa
     6721 tgtgctaaca ttaaacataa ccataacatt caagtcatca atcttttcgt agtcataatc
     6781 ctggtctatc agagaaatca ccacaatcca tttaaatgaa tttgataatc attctcgttt
     6841 agcatagcat gaaacatagc aaaggctatg ttttagaggc acaagatgac gaactatcgc
     6901 gttgagagta gcagcggacg ggcggcgcgc aagatgaggc tcgcattaat gggacctgcg
     6961 ttcattgcgg cgattggtta tatcgatccc ggtaactttg cgaccaatat tcaggcgggt
     7021 gctagcttcg gctatcagct actgtgggtt gtcgtttggg ccaacctgat ggcgatgctg
     7081 attcagatcc tctctgccaa actagggatt gccaccggta aaaatctggc ggagcagatt
     7141 cgtgatcact atccgcgtcc cgtagtgtgg ttctattggg ttcaggcaga aattattgcg
     7201 atggcaaccg acctggcgga atttattggt gcggcgatcg gttttaaact cattcttggt
     7261 gtttcgttgt tgcagggcgc ggtgctgacg gggatcgcga ctttcctgat tttaatgctg
     7321 caacgtcgcg ggcaaaaacc gctggagaaa gtgattggcg ggttactgtt gtttgttgcc
     7381 gcggcttaca ttgtcgagtt gattttctcc cagcctaacc tggcgcagct gggtaaagga
     7441 atggtgatcc cgagtttacc tacttcggag gcggtcttcc tggcagcagg cgtgttaggg
     7501 gcgacgatta tgccgcatgt gatttatttg cactcttcgc tcactcagca tttacatggc
     7561 ggttcgcgtc aacaacgtta ttccgccacc aaatgggatg tggctatcgc catgacgatt
     7621 gccggttttg tcaatctggc gatgatggct acagctgcgg cggcgttcca cttttctggt
     7681 catactggtg ttgccgatct tgatgaggct tatctgacgc tgcaaccgct gttaagccat
     7741 gctgcggcaa cggtctttgg gttaagtctg gttgctgccg gactgtcctc aacggtggtg
     7801 gggacactgg cggggcaggt ggtgatgcag ggattcattc gcttccatat cccgctgtgg
     7861 gtgcgtcgta cagtcaccat gttgccgtca tttattgtca ttctgatggg attagatccg
     7921 acacggattc tggttatgag tcaggtgctg ttaagttttg gtatcgccct ggcgctggtt
     7981 ccactgctga ttttcaccag tgacagcaag ttgatgggcg atctggtgaa cagcaaacgc
     8041 gtaaaacaga caggctgggt gattgtagtg ctggtcgtgg cgctgaatat ctggttgttg
     8101 gtggggacgg cgctgggatt gtagttgaat gagcgtcgca tctggcacta ttggcggatg
     8161 cggcgtaaac gccttatccg ccctacgcgt taaagagccg gtttgtaggc ctgataagac
     8221 gcgctagcgt cgcatcaggc attgtgttcc attggcgaat gtgtttaatg gcggtgtcca
     8281 tgtccgcggc cttttccgcg atgatcgtca cgatcgcgcc agccttcacg atagccacgc
     8341 tcatacgctt tacgcttatc ccagccacga tgatagccat tatcatgacg ccaccagcgg
     8401 tttttacgcc attcataatt gcgatgccag tagtcacggt cgcgccagtg accaccgtcc
     8461 cagtaattac cgtaatgatc gcgatcgcca atttgtaatt tgattgatgg cagtagggtg
     8521 atttcacctg cgtttgctgc aagcggggta aatgccatca gggcggccgc cagaaacagt
     8581 gacctgaaca ttgttattct ccttcacgat cgaagccgtc agcggcctgt taacgcaata
     8641 ttacggggag gtaaagcccc gtatcatcgc cataactctt aaatcataaa ggagagcatt
     8701 ttttgctaaa cgttatcgtg gggtggctgt tgcttcggac gattgaccat catcatcggc
     8761 ttacctgcta ataacagcca tgccgggagc atgacgatac agagcagcgg tagcaatgtg
     8821 gtattgggta caacgactgc cgccataaaa agacttaacc agccatcgcg tgttaccacc
     8881 agcacgatgc cgagaatagc gcaggaaacg gttattgccg ctggaacggc gggaacgtgc
     8941 tcatgcaaca tcaaccctaa tgccacgccg acaaacactg ccgggaagat tcgcccaccg
     9001 cgaaagccac tggcggcagc aacgaccagg gcggtaagtt taatcaccgc cagcaaaaaa
     9061 taatcgctgg tgctgaaagc ctgatttgcc accatctgct gcatctcatc cagcccttta
     9121 aacagcgaaa ccggcccacc aataactccc agaataccga gaataaatcc gccaatacct
     9181 agcacgagca ccggattttt catttgatgc atcatcgcgt gcaaccgtgg taagcaccat
     9241 acggcgacca ttcctgctgc gatggcaatc gccgcgacaa ttgcaccgct gagaatatcg
     9301 gtcatttcca tctgtccgta atgcgcaatg ggcagtgaaa aatggggatg gaaaaataat
     9361 ccggtagtaa gtgcaccagc tgctgccgcc attaatggcg caaaaagacg atcccatagc
     9421 ggaacttcac tactgccatt taaggtttgc gaaaatatca acgccgccgc aacaggtgtg
     9481 ccaaacagcg cgccgatggt tccggcagag gctaaaatag tccactccat tcggttgacg
     9541 cgcggtaaca gacgagcgcc aatggcgacg gcaagggcga tattgacggt catgatcgga
     9601 tgttctggcc ccaggctgac gccgccagca agaccgagaa ttaatgctac gataagtcct
     9661 ggtagcgcag agggcggaac cggtgcgccg atcagcggtt cacaggcggg gtctggtccg
     9721 gcatgaccct ggctgaaacg gataaccaac cccaccgcaa tacccgttag cgttaataca
     9781 ccgatgatcc agaggggtga atcctgggct atccccagag ttcccggcag tcgttgccag
     9841 agcaaattct gtaataccga ggcgattttc atcaccacaa tcagaataag actggacgca
     9901 atcccaattg ccacggcggg gagcgataat aacaacatgg ttctggctcg cggatggagc
     9961 atgttctatt ccttatgcgg ggtcagatac ttagtttgcc cagcttgcaa aaaggcatcg
    10021 ctgcaattgg tgctgaaacg ataaagtaat tgtgtgaccc agatcgatat ttacagggag
    10081 cctgcctttc cggcgttgtt gttatgcccc caggtattta cagtgtgaga aagaattatt
    10141 ttgactttag cggagcagtt gaagaatgac aaagtatgca ttagtcggtg atgtgggcgg
    10201 caccaacgca cgtcttgctc tgtgtgatat tgccagtggt gaaatctcgc aggctaagac
    10261 ctattcaggg cttgattacc ccagcctcga agcggtcatt cgcgtttatc ttgaagaaca
    10321 taaggtcgag gtgaaagacg gctgtattgc catcgcttgc ccaattaccg gtgactgggt
    10381 ggcaatgacc aaccatacct gggcgttctc aattgccgaa atgaaaaaga atctcggttt
    10441 tagccatctg gaaattatta acgattttac cgctgtatcg atggcgatcc cgatgctgaa
    10501 aaaagagcat ctgattcagt ttggtggcgc agaaccggtc gaaggtaaac ctattgcggt
    10561 ttacggtgcc ggaacagggc ttggggttgc gcatctggtc catgtcgata agcgttgggt
    10621 aagcttgccg ggcgaaggcg gtcacgttga ttttgcgccg aatagtgaag aagaggccat
    10681 tatcctcgaa atattgcgtg cggaaattgg tcatgtttcg gcggaacgcg tgctttctgg
    10741 tcctgggctg gtgaatttgt atcgcgcaat tgttaaagcc gacaaccgcc tgccagaaaa
    10801 tctcaagcca aaagatatta ccgaacgcgc gctggctgac agctgcaccg attgccgccg
    10861 tgcgttgtcg ctgttttgcg tcattatggg ccgttttggc ggcaatctgg cattaacgct
    10921 gggaaccttt ggtggggttt atattgctgg cggtatcgtg ccgcgcttcc ttgagttctt
    10981 caaagcctct ggtttccgtg ccgcatttga agataaaggg cgctttaaag aatatgtcca
    11041 tgatattccg gtgtatctca tcgtccatga caatccgggt cttctcggtt ccggtgcaca
    11101 tttacgccag accttaggtc acattctgta aatccttcct gttatatcgg gaggtaactc
    11161 tcccgataat cttttaaatc atacagttaa ttcaattttt ctttgtgtcc cctcacaagg
    11221 ccgacctgcg tcacacttcc gtacagcggg attaattctc cagtaaatgc attatttgtc
    11281 tggtaacggc gatttgtttt gcacgttcat aatttcactc gtcaactatc actgaatgag
    11341 gaattaacga tgagtaagaa actgattgcc ttatgtgcct gcccaatggg cctggctcac
    11401 acctttatgg ccgctcaggc gttggaagaa gcggcggtag aagccggtta tgaagtgaaa
    11461 attgaaaccc agggcgcgga cggtatccag aatcgcctga cggcgcagga tatcgccgaa
    11521 gcaaccatca tcatccactc cgtggcagtt accccggaag ataatgagcg tttcgaatca
    11581 cgcgacgttt atgaaatcac tttgcaggac gcaattaaaa acgctgcggg catcatcaaa
    11641 gaaatcgaag agatgattgc ctctgaacaa cagtaatatt cgacagggat aattgttatg
    11701 gccattaaaa aacgcagtgc aaccgttgtg cctggcgcat ccggtgcggc agcggcagtt
    11761 aagaatccgc aggcctctaa aagtagcttc tggggtgaac tcccgcagca tgtgatgtca
    11821 gggatttcac gcatggtgcc aaccttaatt atgggcggtg tgatcctcgc tttcagccag
    11881 ctgattgctt atagctggct taaaattcct gctgagatcg gcatcatgga tgctcttaat
    11941 agcgggaaat tctccggttt cgacctctcc ttacttaaat ttgcctggct gtcgcagtcc
    12001 ttcggcggcg tgttgtttgg ttttgccatt ccgatgtttg ctgcttttgt ggcgaactct
    12061 atcggtggca aactggcgtt cccggctggt tttatcggcg ggttgatgtc tacccagccg
    12121 acgcaactgc tgaacttcga tcccagcact atgcaatggg cgacctcttc gccggtgccg
    12181 tcaaccttca ttggtgcgct gattatttct atcgtcgcgg gttaccttgt gaagtggatg
    12241 aaccagaaaa tccagttacc ggatttcctg ttagcgttca aaaccacatt tttgctaccg
    12301 attctttccg ccatttttgt catgctggcg atgtactacg tcatcacccc ctttggtggc
    12361 tggatcaacg gcggtatccg tactgtgctg actgccgcag gtgagaaggg cgcgctgatg
    12421 tacgcaatgg ggatcgccgc ggcgacagca atcgaccttg gtggcccgat caacaaagcc
    12481 gcaggtttcg ttgccttcag ctttaccact gaccacgtat taccggtcac cgctcgttct
    12541 atcgctatcg ttattcctcc gattggtctg ggtctggcga ccattattga ccgtcgttta
    12601 accggcaaac gcctgttcaa cgctcaactt tatccgcagg gtaagaccgc gatgttcctt
    12661 gcctttatgg ggattagtga gggtgcgatt ccgtttgcgc tggaaagccc catcaccgcc
    12721 attccgtcgt atatggtagg cgcgattgtc ggctcaaccg ccgctgtctg gctgggcgca
    12781 gtgcaatggt tcccggaatc cgctatctgg gcatggccgc tggtcactaa cctgggcgtc
    12841 tatatggcgg ggatcgcgct gggggcagtc ataactgcgc tgatggtggt gttcctgcgt
    12901 ctgatgatgt tccgtaaagg caaattgtta atcgatagcc tgtaagaagg aaaatccaga
    12961 tgacattact cgcttcgctg cgcgactggc ttaaggcgca acaactggat gcagtgcttc
    13021 tctcctcacg gcagaacaaa cagccgcatc tggggatctc caccggatca ggctatgtgg
    13081 ttattagccg tgaaagtgcg cacattctgg tagattcgcg ctatttcgtt gaggtggaag
    13141 cccgtgcgca aggctaccag ctgcatttgc ttgacgcgac gaacacgctt accactatcg
    13201 tcaatcaaat cattgccgat gagcagttgc aaacgctcgg ttttgaaggc cagcaggtga
    13261 gttgggaaac cgcgcaccgc tggaagtctg aactcaatgc gaaactggtt agcgccacgc
    13321 cggatgtgtt gcggcaaatc aaaacgccag aggaggtgga gataatccgc ctcgcctgtg
    13381 ggattgccga tcgcggagcg gagcatattc gccgctttat tcaggcgggg atgagcgagc
    13441 gcgagatagc cgctgaactg gagtggttta tgcgccagca gggcgcagaa aaagcctctt
    13501 ttgacaccat tgtcgccagc ggctggcgcg gggcgctgcc gcacggcaaa gccagcgaca
    13561 agattgttgc agcgggcgag tttgtcactc tcgattttgg tgcgctgtat cagggctact
    13621 gctctgatat gacgcgcacc ttgctggtga atggcgaagg ggtgagcgcc gaatctcacc
    13681 cgctgtttga cgtctatcag attgtcctgc aggcacagct cgcggcaatc tctgcaattc
    13741 gccccggcgt gcgctgccag cagattgacg acgccgcgcg ccgggtcatt accgaggctg
    13801 gatttggcga ctatttcggt cataacactg gtcacgctat cggcattgaa gtccatgaag
    13861 atccgcgttt ttcaccgcgg gacaccacga cgctacagcc aggcatgttg ctgaccgtgg
    13921 agccggggat ttatttgcca gggcaagggg gcgtgcgcat cgaagatgtt gtgctggtta
    13981 ccccgcaagg cgcagaagtg ctctacgcca tgccgaaaac agtgttgctc acgggagagg
    14041 cataatggat ttatcgctat taaaagcgtt gagcgaggca gatgcgatcg cctcctcgga
    14101 acaggaagtg cggcagatcc tgctggaaga agcggatcgc ctgcaaaaag aagtgcgatt
    14161 tgatggtctg ggatcggtgc tgatccgcct caatgaatcg acaggtccga aggtgatgat
    14221 ctgcgcccat atggacgaag tgggatttat ggtgcgcagc atctcccgcg aaggcgctat
    14281 tgacgtgctg ccggttggca acgtgcgcat ggctgcctgc cagctgcagc cggtgcgcat
    14341 caccacccgt gaagagtgca aaattccagg cctgcttgac ggcgaccggc aggggaatga
    14401 cgttagcgcc atgcgcgtgg acattggcgc gcgcacctat gacgaagtga tgcaggcggg
    14461 aattcgtcca ggcgatcgcg tcaccttcga taccactttt caggtcctcc ctcaccagcg
    14521 ggtgatgggc aaagcctttg atgatcgcct cggttgctac ctgctggtga cgttactgcg
    14581 cgaactacac agcgctgaac tgcctgcgga agtgtggctg gtggccagtt ccagcgaaga
    14641 ggtggggtta cgcggcgggc aaactgccac ccgcgcggtg tcgccggatg tcgccattgt
    14701 gcttgatacc gcctgctggg cgaaaaactt tgattatggc gcggctaacc atcgtcagat
    14761 tggtaacggg ccgatgctgg tgttaagcga taaatcgctg attgcgccgc caaaacttac
    14821 cgcgtggatt gaaaccgtgt cggcagaaat tggcgttccg ttgcaggcgg atatgttcag
    14881 taacggcggc acggacggtg gagcggtgca cttaaccggt actggcgtac ccacagtggt
    14941 aatggggcct gccacccgcc acggacattg cgcggcatcg attgccgatt gccgcgacat
    15001 tttgcagatg gagcaacttt tatctgccct tattcaacgt cttacgcgtg agacggttgt
    15061 tcaactgacg gatttcagat gatctcctga ttaacccgga gcggttatgt taacgattca
    15121 atttctctgt cctttgccta acggtctaca tgctcgtccg gcgtgggaac ttaaagaaca
    15181 gtgcagccag tggcaaagcg aaatcacctt tatcaaccat cgccagaacg caaaggccga
    15241 tgctaaaagc tcgctggcgt tgattggaac tggcacctta tttaatgaca gttgcagcct
    15301 gaacattagc ggcagcgatg aagagcaggc gcggcgcgtg ctggaagagt acatcctggt
    15361 gcgctttatc gacagcgaca gcgttcagcc gacgcaggca gaactgacgg cgcatccgct
    15421 gccgcgttca ttaagccgcc ttaacccgga tttactgtac ggcaatgtgc tggcaagcgg
    15481 cgtcggcgtg ggtacgctga ccctgttaca aagcgacagc ctcgacagtt atcgggcaat
    15541 ccccgccagt gcgcaagatt ccacccggct ggagcacagc ctggcaacgc ttgccgagca
    15601 actgaaccag caattgcgtg agcgtgacgg cgaaagcaaa actatcctca gcgctcattt
    15661 gtcgctgatt caggatgatg aatttgcagg caatatccgt cgcctgatga cagaacagca
    15721 tcagggactg ggggcggcga tcatcagcaa tatggagcag gtttgcgcca aactatctgc
    15781 ttctgccagc gattatctgc gcgagcgggt cagcgacatt cgcgatatca gcgaacagtt
    15841 gctgcatatc acctggccgg aactgaagcc gcgcaacaat ctggtgctgg aaaaaccgac
    15901 cattctggtg gctgaagatt taaccccaag ccagtttttg agccttgatt taaaaaatct
    15961 tgcgggcatg attctggaga aaaccgggcg cacctcgcat accctgattc tggcccgtgc
    16021 ctcggcgatc ccggtactga gcggcctgcc gctggatgcg attgcccgtt atgccgggca
    16081 accggcggtg cttgacgccc agtgcggcgt gctggcgatt aacccgaatg acgcggtgag
    16141 cggttattat caggtcgcac agacgctggc ggataaacgc caaaaataac aggcgcaggc
    16201 cgccgcgcag ctggcctatt cccgtgataa caagcgtatt gatattgcgg cgaatatcgg
    16261 caccgctctg gaagcgccag gcgcgtttgc caacggcgcg gaaggcgttg gactgttccg
    16321 tactgaaatg ctctatatgg atcgcgacag cgcgccggac gagcaggagc aatttgaagc
    16381 ctaccagcag gtgctactgg cagcgggcga caagccgatt atcttccgca taatggacat
    16441 cggtggcgat aaaagcattc cttatctgaa tattccccag gaagagaacc cgttcctcgg
    16501 ctatcgcgcg gtacgtattt acccggaatt tgctggcctg ttccgcactc aactacgggc
    16561 gattttgcgc gccgccagtt ttggcaacgc ccagttgatg atcccgatgg tccacagtct
    16621 cgatcagatc ttatgggtga agggcgagat ccaaaaagcg atcgttgagc ttaagcgcga
    16681 tggcctgcgt catgcagaga cgattacgct ggggatcatg gtggaagtgc cgtcggtgtg
    16741 ctacatcatc gatcacttct gcgatgaggt cgatttcttc agtatcggct ccaacgatat
    16801 gacccagtat ctgtatgcgg tcgatcgtaa taacccgcgc gtatcgccgc tgtataaccc
    16861 gattacgcca tcgttcctgc gcatgttgca gcagattgtt accactgcgc atcagcgggg
    16921 caaatgggta ggcatttgcg gtgaactggg cggtgaaagc cgttatctgc cgctactgct
    16981 tgggctgggc ctggatgagc tgagtatgag tagcccgcgt attccggcgg tgaaaagcca
    17041 gcttcgtcaa ctggatagcg aggcgtgtcg ggaactggcg cgtcaggcat gtgaatgccg
    17101 cagtgcgcag gaaattgaag cgttactcac cgcctttacg ccggaagaag acgttcgccc
    17161 actgctggcg ctggagaata tctttgttga tcaggatttt agcaataaag agcaggcgat
    17221 ccagttcctg tgcggcaacc tcggcgttaa cgggcgcact gaacatccgt ttgagctgga
    17281 agaagatgtc tggcagcggg aagagattgt taccaccggc gttggttttg gcgtagcgat
    17341 cccgcacacc aaatctcagt ggatccgtca ttccagtatc agcattgccc ggctggcgaa
    17401 accgattgac tggcagtcag aaatgggcga agtcgaactg gtgatcatgc tgacgctggg
    17461 tgctaacgaa gggatgaatc atgtgaaagt cttctcgcag ctggcgcgta aactggtgaa
    17521 taaaaacttc cgccagtcgt tgtttgccgc gcaagatgca caaagtatcc tgacgctgct
    17581 ggaaacagaa ttaaccttct gacgttagcc ctgaaaacgg gcgctgtact ctcccggcgt
    17641 cagaccaaac tgacgccgga aaacgcgaca aaaatagtcg ctatccggaa aaccgcaacg
    17701 ctgtgccact tcatgaattg acagatgata tttctgcaaa atcatcctcg ccttcgccat
    17761 tcgcacccaa cgcacatact cgataaaacg catcgttcca tgctgagcaa acagttttga
    17821 cagatgattg ggcgtgatat taaaaaactg cgccacgctc tcgcgggtga gcggctgggc
    17881 ataattatcc tgaacccagt tacaaatgct gtgatagaga aaatcgccgc gcggttgcga
    17941 attacccggc ggcatattca ccacgttgcg gcaaagatgc agcaaactta acaccagtgg
    18001 ctgaatgata ttctgctcca gcggcgaacg gctaagatgg gtgagggcgg taagcatcgc
    18061 ttcaccttca ccgcgttgca ggctgggaag ctggatctgg cgagcaggat gcaacaacga
    18121 cgtggtgcgg ctatcgtaaa acgataaccc aagccaggtc ggtgcgaaca ccaggcttaa
    18181 cagcatcacc ggtttgttgt tgatcggtaa attagccgcg cgtgccggaa taaacagcat
    18241 ttctccttcc accattcgct gctgctttgc ttccagcagg ttgccgtatt cgccgcgcag
    18301 cacgatatcc agacggggaa aatcaataca taaactgccc accggtaacg aggcaggctg
    18361 gctggcaaac cagacgcgcc ccagtagttg cgggttaagc accaggccgc tgaacagatc
    18421 ggcgaaaaat tgttgatcgg cgggcaagcc tggtgctttc atgaaagttc ccgattaaag
    18481 atgcattaac tggcgaaatt ctttcacttt gctgcggctg accggcactt caaaatccag
    18541 atctttcagt cgcagaatgt aggtgttatt aaaccacggt tcgatttcgc gtattttgtt
    18601 cagattgaca caaaatgagc gatggcagcg gaaaaaatgc gacggcggca gtttgctgca
    18661 aaattcggta atgttcatcg gcattacgta ggattcacgc cgcgtataga caaaggtcat
    18721 tttctcgtgc gcttcggcgt aatagatatc gttaattggc gtgacgatta ttcgctcatc
    18781 tttcaccaga ttaatggtgt cattctcacg cgttaccgtc gcggcaggcg tactgctggt
    18841 ctgctgttgt tgccaggccg cttccagttt ttgcagcatc ccggtaatac gtgactcctg
    18901 atacggtttg agaatgtagt caaacgcctc cagttcaaac gcttctaccg catgttcttt
    18961 ccacgcagtg atgaacacaa taaacggttt atgggcgaac tggctgatgt tttgcgccag
    19021 caacacgcca tccagcgacg gaatattgat atccagaaaa atggcgtcga cgcggttatg
    19081 ctgcaaaaac ttcaacacgt ccagaccgtc gtcaaaggtg ccgacaatct ccatctggct
    19141 gtgctcttta attagccagc tcagttcctg ttgtgccagg aattcgtctt caacaatgat
    19201 gactttcaca atatcactcc ggctcaaagc aataacgtag cctgtgaggc gactggggtg
    19261 cgttggttag gaatgtaaaa cgcaatttcc gtccccggct ccaggcggcg gatatgcagc
    19321 ccctcgccat acaataactt cacgcgatga tggacattca gcaggccaat tttattgccc
    19381 ggcatttcat tcgcttcgac ccgctcaatc acctttggat cgatgccgtg cccggtatct
    19441 cgcaccgcaa tgcgtacccg atttccgcac tctgcaacgc tgatggtgac aacgccttta
    19501 cctttgcaag gctgaatacc gtggacaatg gcgttctcca ccaacggctg gatcagcagg
    19561 ctgggaatgc agcaattcac ctcttcatca atatcataga taaccgtcag cttgtcacca
    19621 aagcgggcct gctcaatggc aatatagtct ttaatttgat acagctcttt tttgatatcg
    19681 atttgctcat cgtcttttaa ttcaatgtta tagcgcagat aacgcgacag attaaagatc
    19741 aactggcgag cggtatccgg attcagacgg attgacgatg aaatagcgtt cagagcgtta
    19801 aacagaaaat ggggattaat tttgctttgc agggcgcgaa gctctgcctt atttgccatt
    19861 tcacgtagct gctcggcgcg tgaaacctcc agttgcgtgg agatgatttg cgacagaccg
    19921 accgccattt cctgtaatga cgaggtgatc tgatgcgcgt ggcagtagta aattttcagc
    19981 gttccggtca cgaccccttt ctcccacaat gggatcacca gcatggaatg aatctctggt
    20041 gtgcggtggg cttcatcatt gtttttaatg atgatttttc cgtaattcat cgcctgtcgg
    20101 gtagtcgggc taatgaagtc atcgccattc tgatagttat gttcacccac gccaacgtag
    20161 gccagcacat gatctgtgtt ggtaatcgcc accgcatcgg cgtgaatgtc atcgcgaata
    20221 atttcacaaa ctttgcgtaa tgactcgcta ttgacatggc gaaacagagg tagcgttttg
    20281 ttggcgatat ccagcgccag cttggcctgc cgcgcggcgc tggcctcttt ttcgccctca
    20341 acactttgca ccagaagcac aataaagccg atacagacgc taccgaggat cattggaatg
    20401 ccgattttag agacgatatc gatccccaac gcggtagttg gtgcccagac aatcaccaga
    20461 atcatggtca acgtttcaca caacatgccg ccgagaatac cgacgcgcca gcgctgtgct
    20521 ttggggattt tcaggtttat ccagcctgat atacaacccg ccagaatgct ggtgataaag
    20581 caggggatcg ccgttacgcc gccgatatca attaaatacc ggtgaatacc cgcaattacg
    20641 ccggtaatga tgcccaccca cgggccaaac agaatcccgc ctgacatcac cgcgataata
    20701 cgtacgttca ccagcgagcc ttctacggga acgccggacc aggtgctgaa cagggcgaac
    20761 agcgagaaaa tggcggtaac ggcgagcaac tcttttgggg agtgcgccga cttgtgcaac
    20821 agttcgcgaa acaggcggat acggatgaga aagaacaggc agataagcat taacgctgcc
    20881 cgatcgaaga ccgccagcag catgttgaat atttcgtgca cgggtgactc tgaataagga
    20941 ttaaacctct atgataggta acctgaaggc tgatgaccag caggccgttt ttgaggaaaa
    21001 accacaattt taaaagggga ttgatagata aatggtgtat tagcaatgca tctcttgata
    21061 atgcacttct ggttagtaaa tttattgaaa ttattatgtt aataaaatga gtggtaattt
    21121 tcttattagc gcaggtaaat ttcctgtttt taaagcagag ttcctcattt aacgtcattg
    21181 ataaaaatgt tctaaaacgc tggacagaca gaaattaatc aggctatttt caaaacagcc
    21241 accatcgtgg cagcgtcgct cggacggtcc gggcgctaac gttaatctga ggaaattatg
    21301 gctgacactc gccctgaacg tcgctttacg cgcattgatc gtctcccgcc ctacgttttt
    21361 aacatcaccg ctgaactgaa aatggctgcg cgtcggcgcg gcgaagatat tatcgatttc
    21421 agcatgggta acccggacgg tgcgactccg ccgcatatcg tcgaaaaatt atgtactgtg
    21481 gcccagcgcc cggacacgca tggttactcc acttcacgcg gcattccgcg gttacgtcgc
    21541 gccatttccc gctggtatca ggatcgctac gacgttgaaa tcgacccgga atcagaagcc
    21601 atcgtcacta ttggttcgaa agagggcctg gcgcatctga tgctggcgac gctggatcat
    21661 ggtgacacgg tgctggtgcc gaatccaagt tacccgatcc atatttatgg cgcggtgatt
    21721 gccggggcgc aggtacgctc agtgccgctg gtggaaggtg tcgatttctt caacgaactg
    21781 gagcgcgcca ttcgtgaaag ttatccgaaa ccgaagatga tgatcctcgg cttcccgtcg
    21841 aatccaaccg cgcaatgcgt ggagctggag ttcttcgaaa aggttgtggc gctggcgaaa
    21901 cgctacgatg tgctggtggt ccacgacctg gcctatgccg atatcgtcta cgatggctgg
    21961 aaagcgccgt caatcatgca ggtaccgggt gcacgcgatg tggcagtcga gttctttacg
    22021 ctgtcgaaaa gctacaacat ggcgggctgg cgtatcggct ttatggttgg caacaaaacg
    22081 ctggtcagcg ccctggctcg tattaaaagc tatcacgatt acggcacctt tacgccgttg
    22141 caggtggcgg cgattgcggc gctggagggc gatcaacagt gcgtgcgcga cattgccgaa
    22201 cagtacaaac gccgccgtga tgtattggtt aaagggctgc atgaagcggg ctggatggtt
    22261 gaaatgccga aggcttcgat gtatgtctgg gcgaaaatcc cggaaccata tgcggccatg
    22321 ggatcgctgg aatttgccaa gaagctcctt aacgaagcga aggtttgtgt ctcgccgggg
    22381 attggctttg gcgactatgg tgatacgcat gttcgctttg cactgattga aaaccgcgac
    22441 cgtattcgtc aggcgattcg tgggattaaa gcgatgttcc gtgccgacgg tttactaccc
    22501 gccagcagca aacatattca cgaaaacgcg gaataaacac cgtcgtcaga aatgcaaaca
    22561 ggagcctgat ggctcctgtt tttcattgca cagtttgctt ttagatcatc agggcaaaag
    22621 taccggccca aacgatgacc ataaaagaaa tgcccataaa gaaatatttc actttacatc
    22681 tctccgcgta attactgtta cgcctgaatg acttcattac acctgataca gtgagttgac
    22741 accgggcaaa gcgaccttaa aagccctgta ttttaccgtg ggcatcaccc cagaattctg
    22801 agctaaactg ctcgagacgg cgctaatgta cgcctaaaac tgaaacgaga caagaggcat
    22861 tttcttattt acttttagta acttacaagt gtctcatatc ggcgacagtt taatttctga
    22921 cggtaataat tagatattca gcgaaggaaa aaaccttaag aaaaaagcca taaaaaccat
    22981 gaggttatta tggccgattt gaggagggaa agagtaagag cagtttgtta aatgtacaac
    23041 gacgattctc ccaccgggcg cgttttaaag cgacggtgga tccagaggta ctgctccggt
    23101 gcgcgcatga tctctttctc gataatcttg ttcatatagg cagcggcttg attttcatct
    23161 gtcgggtagc cttccatctc tggggtgatg aacaaacgat atccgctgta atccgctttt
    23221 cttaccatcg ttacggtcaa catggctgcg ccagagagac gggagagaac ataggtgcca
    23281 ttggttgtgg cgacattttc caccgcaaag aacggcgcga aggagctgcc tttacgacca
    23341 taatcctgat cgggagcaaa ccataccgct tcacctttct tcagtgcacc gacaatgccg
    23401 cgcagattat ttctgccgat catcgctttg ttagagcgca tgcgcccacg ggtctgcacc
    23461 cattccatca gctgattatt atgtggacga taggtagcca tcattggttg gcacagtccc
    23521 atcacgcggc cgcccagttc cagcgacata aaatggacgc cgacaaccat tacgccgcga
    23581 ttttgcattt gtgcgcgttt aaggttatcc aacccttcaa catcaaacca tttacgtacg
    23641 cgactgtcgg gccagaacca tgccatgccg gtttctacca gcgccatgcc gagtgaacga
    23701 aagttttcgg caatcatctt ctcgtgttct tccgcagaat gctgcgggaa acaaagttcc
    23761 aggtttttac gggcgataga ttcacgacgt ttcaggaatg gtcgcgccat tgcgccaata
    23821 cgcgtgccga gaaagcagag aacaggataa ggaagctgta cccaaagcca gagtacacca
    23881 agcccaaacc atgtgagcca gtagcgagga tgcagaaact cgcgggaaaa tttgcattgt
    23941 ggaaacatag ttaccttctg tagatacgaa ttgttgtatc gcagaagatt ccatgtcgac
    24001 agcggtacac tcaattgatt aggccatcat gtacgacaat ggttcctgta aagagtgtaa
    24061 agactagtca ggacataacc acgaggagca tgaatgcggt atctgtactt tatcactcgc
    24121 tgactgcttt cagctactga ttattaatcg tgcaaagtaa atatttttcc gtacacaaca
    24181 gtcttgcatt taacatgctg ttttcattga tgcttagcaa aaattgccag aggttaagga
    24241 ttgtcttact attaaagtga ctgaagttac aggtttttta gcgcggcagg aggttgagct
    24301 atgatttatt tatggatgtt tctcgccctg tgtattgtct gcgtaagcgg ctacatcggt
    24361 caggtactga acgtggtatc ggcggtctct tctttcttcg gcatggtgat cctcgctgca
    24421 ctcatttatt acttcaccat gtggttaact ggcggtaatg aactagtgac ggggatattt
    24481 atgtttcttg ccccggcttg tggcttgatg attcgcttta tggtggggta tggcaggcga
    24541 tagtgggtca ggatggctca tttgggcagg tgttcacatc agcatcagac atgattgttc
    24601 acctgccatg tgacagacgc tatcgcacag gatgaataag ataattcgcc tttgtcggat
    24661 aagtaggcgt gatttcttct gtgaggttta atggtggcgc atcttctctc atcttaaaca
    24721 aaatcgcctg tgatggctga agttttgtag agtgtgtggt cgtcatcgct gatgtttttt
    24781 cagtctcttt atagctcctc tcacttacac caccagaaat ggaaaatgca aaaacattgc
    24841 atgccataat agagaccaat aaaaataagc tgaaaagtat taagtttact ttcatagaaa
    24901 gttatcctga aaatggttca ctgagaacct acgatgttat ggctgtattt acatataatt
    24961 aatcaatatt tacatcgata taataaa
//
LOCUS       DAJSKJ010000045        24793 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000045 DAJSKJ010000000
VERSION     DAJSKJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.045"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(33..857)
                     /locus_tag="ON345_001552"
     CDS             complement(33..857)
                     /locus_tag="ON345_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013577762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071634.1"
                     /translation="MEEDSQPEKNITIQEPLFSLEIVDYKPAMGTAHLRHTVMASDGL
                     EYAIKGVSDGDASALTGIPHPKQIPASEWLCTKLAEFSGLPTPACRVLIDPDTGEHFF
                     GSKYDLAAMSKPQEEINFAAELMSGPSILRKQVWSVYAFDQFVFNIDRHIGNYLYTKN
                     RYGNLTIQAFDFSLAAMVMGWPNKTDAALIPMGYNTTNCWQAIKQLTYNDVSCKAPAE
                     AVLDKLSRISSSTIERIFEDMPEAWINPLQRDALLTWWSSDGRMKRIDTVRGEVLK"
     gene            complement(1167..1865)
                     /locus_tag="ON345_001553"
     CDS             complement(1167..1865)
                     /locus_tag="ON345_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3071635.1"
                     /translation="MKKETLADRLNEAMASAGMSQGALAKASGIAQPTIWRLVSGNAR
                     GSTKIVEIANALGVRSEWLSTGNGPMRDDGQLPRAAQVKSQDTDAFRIDVLDLMVSAG
                     PGIVNQEFVEILRSVEYEPAEARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDI
                     SVKSFDGDGIYAFLYDDTAHVKRLQKMKDKLLVISDNKSYAAWDPIEKDEMNRVFVFG
                     KVIGSMPQTYRKHG"
     gene            1977..2204
                     /locus_tag="ON345_001554"
     CDS             1977..2204
                     /locus_tag="ON345_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3071636.1"
                     /translation="MTNKTIQKAIDIAGSQKKLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATNGKIKAAEIRPDLAQLLSAHSPAA"
     gene            2245..2466
                     /locus_tag="ON345_001555"
     CDS             2245..2466
                     /locus_tag="ON345_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3071637.1"
                     /translation="MESSTTRNKVEARRIESWLHSQIAELGTTNIAKVAGVNKSTVSR
                     WRESLLPNMSLLLAILISNRPGEKGDFEA"
     gene            2552..3406
                     /locus_tag="ON345_001556"
     CDS             2552..3406
                     /locus_tag="ON345_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3071638.1"
                     /translation="MANTAEVINFPVPVVALQELRVADLDDGFTRIANELLEAVMRAG
                     LSQHQLLVFMAVMRKTYGFNKKSDWVSNEQLSELTGILPHKCSAAKSVLVKRGILTQT
                     GRVIGINKTVSEWSSLPVKGKEKKPYLKKVTLPESGKKSLPESGNAYYPNQVNTKDKH
                     TKDNKDNINNPPKSPRAVSFDALAVQLPDWLSAEIWSSWVAYRRDLKKPIKSQQTVTQ
                     AINLLDRCRLNGYAPEEIINRSIANGWQGLFEPNGAKPQPSQQVRVAENFAGKDYGQT
                     EIPSWARD"
     gene            3391..4260
                     /locus_tag="ON345_001557"
     CDS             3391..4260
                     /locus_tag="ON345_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3071639.1"
                     /translation="MGEGLIMELEEKITAIERMLDQLSKPPEDIPNCEVVIERVCCEK
                     HGEYEQRKRILTSSIINLPSPPTRCPGCLEDELNFLKDEKVRWDKRVRQQTAERLLRQ
                     LDIPERFSTCTLDSYKPVGKDSERALRVCQAYASKWTDRLQQGGGLVMCGKPGTGKNH
                     LALAIARHVIEHHQSSVIFTTALKIAREFKSTWSKTATRTEDEVISYFTKPDLLIVDE
                     VGVQFGSEAEKMIMFEIINTRYERLKPTILISNLPKDELTQFIGERVIDRMNDGGGCT
                     ISFTWDSYRENRS"
     gene            4257..4550
                     /locus_tag="ON345_001558"
     CDS             4257..4550
                     /locus_tag="ON345_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HCR3071640.1"
                     /translation="MTGKDAILNYLKTHKTCSSPDVAAASGMTHTCINQAANILAKQG
                     VLVAEARVWRTVYYRLATEEEISGRKSTNQIFNECRQSPAMKRVLAVYGRTSA"
     gene            4553..4852
                     /locus_tag="ON345_001559"
                     /pseudo
     CDS             4553..4852
                     /locus_tag="ON345_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4959..5528
                     /locus_tag="ON345_001560"
     CDS             4959..5528
                     /locus_tag="ON345_001560"
                     /inference="COORDINATES: protein motif:HMM:NF016343.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR3071641.1"
                     /translation="MTKSTITRDELNEVIATYGKHHIAHRMANVLLAAMDSEPDCKER
                     KLFCSTDTTRMRKTISVSAGTEDAPLYRHAQQPVNSEPVGYLFHNEYGVVLYSISDDA
                     TEGFSLIGPIYSVPQPAPVVPGKWIPVSEQMPEDRTQVILWDAEIGEVTSGHYSHKTH
                     TFYHCGDAIENEITHWMPTPAAPQEMKGE"
     gene            5521..5760
                     /locus_tag="ON345_001561"
     CDS             5521..5760
                     /locus_tag="ON345_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071642.1"
                     /translation="MNKAELLQKISALATECHTLACDLDIGDERTEMFEIYGVLHNLG
                     RRGYACQVGRRMNPLLASCDDDEDEEDDDWDEDDD"
     gene            5844..6026
                     /locus_tag="ON345_001562"
     CDS             5844..6026
                     /locus_tag="ON345_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071643.1"
                     /translation="MTNKKMTPAEKLKASRKRYKKIWLQMDIANAKRFGEKEVLSVDT
                     YRSPYETRKKRGRTAD"
     gene            6023..6286
                     /locus_tag="ON345_001563"
     CDS             6023..6286
                     /locus_tag="ON345_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR3071644.1"
                     /translation="MTTSSWNIAAKSKDEQDKVNVDLAASGVAYKERLNMPVVAEVVA
                     RQQPEHLRDYFMERVRYYREQSIQLPRASDPRYLEMAEQNAKK"
     gene            6393..6989
                     /locus_tag="ON345_001564"
     CDS             6393..6989
                     /locus_tag="ON345_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR3071645.1"
                     /translation="MAQTLQFEKSYQNVLIPAEPGTSEYLQLIPVGQLLCGEFRKPRN
                     YAFHKKFFKLLTLGYHYWTPSGGLIEPAERTLISGFIDFLSSDLDQRAALQNAAEMHL
                     SSVGISRSRDMALLKHFESFREWATIQAGFYDEYQMPDGSRRRVAKSISFASMDDSQF
                     NGVYKSVLNVLWNYILRRKFHSPAEAENAASQLLSFAG"
     gene            7198..7488
                     /locus_tag="ON345_001565"
     CDS             7198..7488
                     /locus_tag="ON345_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR3071646.1"
                     /translation="MANLRKAARGRECQVRIPGVCNGNPETTVLAHIRIAGLCGTGIK
                     PPDLLAAIACSSCHDEIDRRTRLVDAEYAKECALEGMARTQVIWMKEGLIKA"
     gene            7485..7847
                     /locus_tag="ON345_001566"
     CDS             7485..7847
                     /locus_tag="ON345_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR3071647.1"
                     /translation="MNQYRISLPWPPSNNRYYRHNRGRTHISAEGQAYRDSVARIIKD
                     SMLDIGLATPLKIRIECHMPDRRRRDLDNLQKAAFDALTKSGFWLDDQQVDYYSVKRM
                     PVVKGGRLELTITEMESA"
     gene            7844..7984
                     /locus_tag="ON345_001567"
     CDS             7844..7984
                     /locus_tag="ON345_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCR3071648.1"
                     /translation="MSRDVIERIRDRWQKLRLLRSRGTVLVDYKILRNFVRIYKRLGE
                     TA"
     gene            7981..8481
                     /locus_tag="ON345_001568"
     CDS             7981..8481
                     /locus_tag="ON345_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR3071649.1"
                     /translation="MKLELTNEQHQWIDQWLQLWGAWCQTGKIDKAMINMIAKFMATV
                     EPQAPSRPVCSDDDGLLIDAVIRHYLKNVDENAWKVIFAYYVCNSSEIRIASWQHAVS
                     KPRLMKTRAGNQYKHPSISTIRREVKQIINAALFCLYQPLQNAFNDRESVRKIAKNSH
                     NVLAFQ"
     gene            8576..8650
                     /locus_tag="ON345_001569"
     tRNA            8576..8650
                     /locus_tag="ON345_001569"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8608..8610,aa:Asn,seq:gtt)
     gene            complement(9587..9757)
                     /locus_tag="ON345_001570"
     CDS             complement(9587..9757)
                     /locus_tag="ON345_001570"
                     /inference="COORDINATES: protein motif:HMM:NF021415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HCR3071650.1"
                     /translation="MIIKPCPYCGKLINPESLVCSHCRIVNPFVKASRREKAKNVLVI
                     ALVAAFLIWMIL"
     gene            9963..10241
                     /locus_tag="ON345_001571"
     CDS             9963..10241
                     /locus_tag="ON345_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR3071651.1"
                     /translation="MTRMSTIYSRLSYGTGTTLTGCGVSAKAYAETAKTAKEVSWMLA
                     DRIAGLSLSDWAIIVGIACTVITCAVNWYFRWKEREDRRNGYATKAEE"
     gene            10219..10785
                     /locus_tag="ON345_001572"
     CDS             10219..10785
                     /locus_tag="ON345_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR3071652.1"
                     /translation="MPPKLKNSVIAAIPAGAIAIAAALITGPTGNDGLEGVRYQPYRD
                     VVGVWTVCWGHTGKDIILGKTYTKAECQALLDKDLNIVARQINPYINAPIPETMRGAL
                     YSFAYNVGAGNFKTSTLLRKINQGDSKGACEQLRRWIYAGDKQWKGLITRREIEREVC
                     LWAEKPQVLGDGLGPLNPGIPVSVPGVF"
     gene            10785..11327
                     /locus_tag="ON345_001573"
     CDS             10785..11327
                     /locus_tag="ON345_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071653.1"
                     /translation="MKPRKITIVAVLLVAVVIIIAVLSVLLVRSRSALETEQSENRVL
                     RNDNALQATVITTQAFNFNRFNQIAENANRLNSLIDAGTEKTVIEYREILRREKTCDL
                     PVPADVAGGLLKYAYRLRASAMHPDTGNTNATDDSTTAASSMTYCQAVLWIEPLLATI
                     EKGNNNFSGIREIENNRAMR"
     gene            11412..11711
                     /locus_tag="ON345_001574"
     CDS             11412..11711
                     /locus_tag="ON345_001574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071654.1"
                     /translation="MIFPIVKSVYINSKTDSRLIRYDVIRTDPDTYLVKVFDNQQRGI
                     SVPGLISQISEFEITRAAYNEKYQVGNRVVVKMNADPGFENTIDGELQAHRDSLS"
     gene            11760..12398
                     /locus_tag="ON345_001575"
     CDS             11760..12398
                     /locus_tag="ON345_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071655.1"
                     /translation="MAALKNLIQQLNQMQKQIPFATAQAMTKVVRQIETAQKTAFERH
                     LESPTPFTVKSVGSVAARKNNLTAKVFVRDTAAGYLEPFEFGGEHKLNSQALLNPKNV
                     KLNKYGNMPRNKLSQLKAKENVFVGEVDGINAVWQRKKPMKAKKRRAKRSANGTRRPK
                     RKQRSPKLLIRFGDALPVTPVLGYMDRARTMANALLPSALNQAIAEAIRTAK"
     gene            complement(12409..12912)
                     /locus_tag="ON345_001576"
     CDS             complement(12409..12912)
                     /locus_tag="ON345_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071656.1"
                     /translation="MGNNIIKITFVAAVLASPIAHAQWVTNTEDDLFSGGKKAMMLGE
                     VSSDNGAIVFDCTKEKLSAAYVEMDKSTESLSEVPMDLIMKVDGNTAVKLDATLSRRN
                     VQSLQIQSDDTDQLKTVLKQLQGAKSKVLVGVQTKDGGNQHSMSANVSGSTTAVNSFI
                     KACEISL"
     gene            13202..13771
                     /locus_tag="ON345_001577"
     CDS             13202..13771
                     /locus_tag="ON345_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071657.1"
                     /translation="MTTMNQSQYAQHSGVDRKTIGRWIKAGRFIVMDGDLIDVEASDA
                     ALKKNRDGKDPRASNAKKKKTPVVSDNDDDGDEINKTVRQIMLTEGADLSREEAGRIR
                     ENYMALQAKLQYEKDSGQLIELTAAEEVLFNAFRQQRDAWLNWPSRVAPLMAADLDVP
                     ADRMTEVLIEHVHKHISVLGEPEFNPAED"
     gene            13713..15836
                     /locus_tag="ON345_001578"
     CDS             13713..15836
                     /locus_tag="ON345_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR3071658.1"
                     /translation="MSTNTSQSSESQSLTRQKIERLELSVRKGWTPPPRISVPQWADD
                     YRKLAKEAGSTSGNWETSTVEIARGPMLAATESGVHIITVMCCTQLMKTALLENLFGY
                     FAHLDPCPILLLQPKEEAAEQFSKERISPLVRVTPVLRKIIGDSKQKSSKETILYKAF
                     TGGFLALAGAGSPDNLARRPIRVLLADEVDKYPITREGDPIALAEERTATFGLTWLSV
                     RACSPTVEDESRIADSYADSDQRRASVVCPHCGHRQFPDFFKHVQWPKEGDKHLTKSA
                     MLYCECCGSGWSEGQRLRALHTIRWHQTRPFECCGERHSPLMDYDLAWRAADEGSVEK
                     VWQWSESERHAVYRAICPSCGKEAVDNHHAGYQASKLFSPWQKDKPSDIAKKYIDAKG
                     DPDKEQAWWNTQMGLPHRPNHGKQLPVDVLLARREIFPAVVPDGVALLTAGVDTQDDR
                     FEITITGWGRDEESWSVAHDVIYGDLETEEPWKRLDAYLKQIWRRGDGRGLNIMATCM
                     DSGGHHTQKVYEFAKERLGRRVWAIKGESAQGGKRNPVWPTKRPSSKSKASFRPVILG
                     VNSAKDVIRGRLHLEPPKPGAAAAGYMHFPDDRDLGYFNQLLAERLVYKVISGQRYSI
                     WEAIPGRANEALDCLVYSYAALCGLKHMGLKLNVRAANLEADPDKFLPAPVGQEEKIN
                     YELPGAVIEEPAPVKRKRISQLLPK"
     gene            15845..16108
                     /locus_tag="ON345_001579"
     CDS             15845..16108
                     /locus_tag="ON345_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3071659.1"
                     /translation="MFNRNTSLLAGAMTDDQLRDALAKAQQAYIDLATGSHGVSFSYT
                     QGDGTRSVSYQQSTLADLLALIQLLQAQLGIISRPRKPARFRF"
     gene            16108..17745
                     /locus_tag="ON345_001580"
     CDS             16108..17745
                     /locus_tag="ON345_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3071660.1"
                     /translation="MNKVQILGSDGQPLRQQRPSMLVGGSRVPYDAADSFSDQLANWQ
                     PALWSPDNEINIYRDRIVSRARDLVRNDGWANGAVTRLLDNAVGANFRPIMKPDYRVL
                     RMITGNKAFDASWAEEYGKALDGHWRTWSNDPGRYCDVERKLTVSQMLRLGFRHKLID
                     GDALAILQYRTDRLGPGRGRYATTVQIVDPDRLSNPQQNFDMPNVRGGVEIDADGAPV
                     AYHIREAHIGDWWSGAKTMTWQRIPRETDWGRPHVVHDFDHERGAQHRGNGILTPVIQ
                     RLKMLVKYDQSELEAAILNAIFAAYIESPYDPAMVQSALGETYDESELGTYQDGRVEF
                     HNDRRLTLQNGARMPILYPGEKITTVNAARPYSNFEVFESAVLRNFSSGTGLSPQQVT
                     QDWSDVNYSSARSSLLEAWKTLTRRRDDFSTGFAQPILTAFVEEVHDNEDLPLPAGAP
                     DFVDARAAYSRARWMGPGRGWVDPVAEKKGAILGLDAGLSTLEIEVGENVGEDWEEVL
                     DQRQREIESCLKRGLPLPSWAQADQFASQTITDPEEK"
     gene            17742..18608
                     /locus_tag="ON345_001581"
     CDS             17742..18608
                     /locus_tag="ON345_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR3071661.1"
                     /translation="MNLPHLAQRLFNTPLALHPSKAEVIMASVMDRFGISKIESSLAM
                     EDDWYGYDDNRGRESRSDPGYDNVLGVAVIPICGTLVQKLGSLRPYSGMTGYDGIRQA
                     FLTAMEDPDISGICLDIDSPGGEVAGCFDLVDVIYGSRGKKPIHAILTESAYSAAYAI
                     ASAADRISVPRTGGVGSVGVITMHLDWTQRIKDDGLKVTIITYGSRKAEGSPLRELSD
                     EALAAIQQDINTMGELFVNTVARNRGISAKVIKSTQAACFMAADGVEIGLADEVCPPD
                     AAFKNLLEKTGA"
     gene            18610..19200
                     /locus_tag="ON345_001582"
     CDS             18610..19200
                     /locus_tag="ON345_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071662.1"
                     /translation="MAKKKTFSFAHLIGLGPSASEEEEDKKAKKAKARRAEEDEREDD
                     ADDDERDDDAEEDERDDDAEDDGDDPDASEDDDSEDDGDDDRKESKAVKNARAAERKR
                     CARIFGSKHAAANPSLAASLAFNTGMSSAAAINVLASSAPAAAASQPSRKRSLDQRMQ
                     ESHQVRLNPDSGQKETGKSALVSKMTGLYNSTRGEK"
     gene            19200..19604
                     /locus_tag="ON345_001583"
     CDS             19200..19604
                     /locus_tag="ON345_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR3071663.1"
                     /translation="MDQFGQNAFAPGMKSALFVPDQLVAGTLQLVTDTGIITGGAFKR
                     GTVLGLVAASGKYTQCVKTAEDGSQLPVAILVDDVDASSSDQNGGLYLMGEFNQHRII
                     FDNSWTIADLKKALRPLAIFLKDSDQAPVTTS"
     gene            19702..20751
                     /locus_tag="ON345_001584"
     CDS             19702..20751
                     /locus_tag="ON345_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR3071664.1"
                     /translation="MENIFDTSVLVQVVPNLKTSQNWLLDRFFPNVVTYETEEVAIDV
                     DVGLRRMAPFVSPLVEGKLVESRKYQTNTFKPAYIKDKRAPDLRKPIRRQIGERIGGE
                     YTAAEREMLNLQFEMTDQIDMINRRLEWMAASALVSGTVTVAGEGYETKVVDFGRSPD
                     LTITLSGSDKWPLTVAAGATNTQPSDDIEIWQTLFLKESGSVATDLVFTSKSWRAFRL
                     DTTIKDNAITFPALSPFGNQINAGPQAMKGAIYKGRWGNFDLWLYNDWFIDPLDNVEK
                     PMIPDGAVIMSGADLMGTRAFGVILDPAFNYGPLAYAPKSWVKEDPAQRLILMQSSPL
                     VIPSRVNASLCATVV"
     gene            20723..21133
                     /locus_tag="ON345_001585"
     CDS             20723..21133
                     /locus_tag="ON345_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071665.1"
                     /translation="MHPFVQRWSDMAKTTKTVLGDDLNAEGATEDALNIDDLNAGGSV
                     HETQQHDDKHGEPSDDEDTAEEDDQEDAPEPEFVVLKGNCIRHDGEVYRENSLIPVSG
                     KDAERLLAAGVIADVHALRQRALSAVRGVKITTE"
     gene            21138..21497
                     /locus_tag="ON345_001586"
     CDS             21138..21497
                     /locus_tag="ON345_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071666.1"
                     /translation="MGVDWDLHLLSPLHGVFGDEHEYRPRNGTPFTINGIFDRGYAQI
                     AENLDGESEINTSSPMLGVRDAEFRQLGKPQPAVSDRVFIKTVGGQVINQLFVVSNVE
                     PDSHGGSRLVLNVAKTR"
     gene            21494..22039
                     /locus_tag="ON345_001587"
     CDS             21494..22039
                     /locus_tag="ON345_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3071667.1"
                     /translation="MNASAIRQMVVAALKDKTAAGDRVYSPRDWATSPDLYPALLVQT
                     PFDHKKAQGRNTPAFTSLTTVRITGRVQEYDSETTDDGAMRAEVALEELREQVERAVI
                     NSYELTRNIQKYAEVRSTIDVDADGEAHMGQLLIEIDIEHYQGPEDFYPIDTVPLAGI
                     DITINMPDGTPQPGVKIDLQE"
     gene            22042..22239
                     /locus_tag="ON345_001588"
     CDS             22042..22239
                     /locus_tag="ON345_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR3071668.1"
                     /translation="MFVKPKDGISVRCPVRGIPLPKEGAEVPDNIFWRRRLSDGDVIL
                     SKKDEGAPEKQSLPKKAGENE"
     gene            22236..23738
                     /locus_tag="ON345_001589"
     CDS             22236..23738
                     /locus_tag="ON345_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR3071669.1"
                     /translation="MTVPFARVPDNLRVGLFFVEFDNSMANNATATQRTLLIGGMLST
                     GSTPPGIPQRVSSSDTVGELTGKGGILQAMMAAYQKNDTAAEVWILPLEEDSDSMVAA
                     TGTIKVSSAPTATGVISLYIAGERIQLTVVATDTVAAIATSLAAAINAKTTLPVTASA
                     TTDTVTLTAKNLGATGNGIDIRLNFLGLPGGESTPAGLELTITAMSNGVGAPDITGAL
                     ANLQDRTFDFIINPYDDTTSLNVMKEFLSDTGGRWAWDKQLYGHSFGTTTGTYAQLGT
                     KGELRNNQHETLLGVNKSPSPSWAWSAAYTGAAAVSLRNDPGRPLQSLAVQGVLAPEL
                     QDRFELTERNNLLYSGISTFTVDDDGTVRIENLITTYQKNSYGDADDSYLEVETLFSL
                     MFVTRYLRTAVTSKFGRMKLAADGTRFAPGAAIVTPNIIKADQIAEYQTLVWNGYAQD
                     AEAFAKNIIVEQNAKNPNRVDVLWPGTLMNQLRIFALLNQFRTRAESTGA"
     gene            23742..24113
                     /locus_tag="ON345_001590"
     CDS             23742..24113
                     /locus_tag="ON345_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR3071670.1"
                     /translation="MAGDTTNRLAGTAYVTVNGVTVMVEGSFKYQAATVNRTTLTGMD
                     GVHGYKEKPVAPYISARLRDSGGTNVQGFNQQTNVNVIAELANGKTIIGRSLWTVNVQ
                     EVESEDAVFDVRWEGRDVTEN"
     gene            24115..24393
                     /locus_tag="ON345_001591"
     CDS             24115..24393
                     /locus_tag="ON345_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR3071671.1"
                     /translation="MAEIERVKTIPLTVALDDAAEKTTYTQLELKAPTLSQAEQFYEK
                     QAASTSLAAMRLLIALVSGTRESVLQPMDFLDFRKCEEYLLSFLTWKP"
     gene            24535..>24793
                     /locus_tag="ON345_001592"
     CDS             24535..>24793
                     /locus_tag="ON345_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071672.1"
                     /translation="MSNSFDFELVASDQVSEAIDRINEAVRDLEPKLDKTKEGLKLGG
                     QETADGLSGFISRLENMSKSARDNVQFIGDMVPPLKMVGELT"
BASE COUNT         6258 a         6202 c         6684 g         5649 t
ORIGIN      
        1 tgttattctg acaatactgt atttatatgt attcatttca gcacctcccc tctaacagtg
       61 tcgattcgtt tcattctgcc gtcgcttgac caccaggtta gcagagcatc cctctgcaaa
      121 ggattgatcc atgcttccgg catgtcttca aaaatccgtt ctatcgtgct agagctaatt
      181 ctggatagct tatccaatac agcttccgca ggcgctttac acgaaacatc attatatgtc
      241 aactgcttga tcgcttgcca gcagtttgtg gtgttgtagc ccattggtat tagggctgcg
      301 tcggttttgt tcggccaacc cataaccata gctgcaaggc tgaaatcgaa ggcttggata
      361 gtcaggtttc catatcggtt cttagtgtac agatagttac caatatgcct atcaatatta
      421 aatacgaact gatcgaaggc gtaaactgac catacttgct tccgtagaat agaaggtccg
      481 gacatcaatt cagcggcaaa gttaatttct tcttgtggct tactcatggc agccaggtca
      541 tattttgatc caaaaaagtg ctctcctgta tccgggtcaa tcaaaaccct acaggccggg
      601 gtaggaagcc cactaaattc agcaagttta gtacatagcc attctgatgc tggaatttgc
      661 tttgggtggg gaatccctgt caaagcggat gcatcaccat cagaaacccc ttttattgca
      721 tactctaaac cgtcggaggc cataactgta tgccttaggt gagctgtgcc cattgctggc
      781 ttataatcca caatctcaag actaaacaag ggttcttgaa tagtaatgtt cttttcaggt
      841 tgtgagtctt cttccatttt tattccacta attgattagt gatagattcc accgggtact
      901 tcattgctgc tctgaactta agctttattg cccttctggg cggcgaggaa ccgcgaagcg
      961 ctcttatcca tgcttcctgt acgtcttcgg cattaccaaa aacacatcga actaccggtc
     1021 tggctcactc aaagtcatcc cgctcatcct tccgcttgaa gaaaacttta tccagcctga
     1081 gcactatccc aaccagtccg ataatcagca aagtaatgag tattgggata atcagatcag
     1141 acatgcttcc tctgcgtgct aaggctttac ccatgcttcc tgtacgtctg cggcatgctg
     1201 ccgatcacct tgccgaacac gaaaacacga ttcatttcgt ctttctcgat cgggtcccag
     1261 gctgcatagc tcttgttatc tgagataacc agcagcttgt ccttcatctt ctgcaggcgc
     1321 ttgacgtgag cagtgtcgtc gtacaggaag gcgtatatcc cgtcgccgtc gaagctctta
     1381 acgctgatgt cgacgaacag cagatcaccc ggctcaatcg tgccggacat gctgtcaccc
     1441 cggacgttga tgatccggat gttctcagcc ttacgcccat cgaacatgtg ccgggcttca
     1501 gctggctcat actcaacaga acggagaatc tcgacgaact cctggttcac gatgcccggc
     1561 ccggcactaa ccataaggtc cagcacatca atcctgaatg catcagtatc ctggctttta
     1621 acctgggcag cgcgagggag ctggccgtca tcgcgcatcg gtccatttcc ggttgataac
     1681 cattcagacc tgacgcccaa agcattagct atctcgacaa tttttgttga acccctggcg
     1741 tttccactca ccaggcgcca aatggttggc tgagcaatac ctgaggcctt cgcaagcgcc
     1801 ccttgtgaca ttccagccga agccatggcc tcgtttaaac gatctgcaag agtttctttt
     1861 ttcataatct caaatttata cgcttgcgta ttactggtca aaacacgttt tgctattgct
     1921 aaaaccaata cgcattgcta ttatcatttt gcaccaatac ttataggaat tggaacatga
     1981 cgaacaaaac catccagaag gcaattgata tcgctggcag tcagaaaaaa ttggccgatc
     2041 tgtgtggcgt agcgcagccg acagtttggc gctggctgca tggcggcgga attgacgctc
     2101 gctatgtaat gaaaatcgtg tctgcgacca acggcaagat taaggcggca gagatcaggc
     2161 ctgaccttgc tcagttgctg agcgcgcatt caccggccgc ctaaccagcg gccattccaa
     2221 acaacaccag aggaagtatc acaaatggag agttcaacga cacgcaacaa agtggaggct
     2281 cgcaggatag aaagctggtt acacagccag atagctgaac tgggaaccac gaatatcgcc
     2341 aaagtggccg gagtgaataa gtcgacggtg agtcgctggc gggaaagtct gctgccgaac
     2401 atgtcgctgc tgctggccat cctgatttct aacaggccgg gagagaaagg tgattttgaa
     2461 gcatgagtgg gaacagaaag gcgaaagccg cagtgcagca acactaacgg ctttctacgc
     2521 gaattaactg gatcaattca caggagtaat tatggcaaat actgccgagg taatcaattt
     2581 ccctgtgcct gtcgtggcac tacaggagct gcgcgtggca gatctcgacg atgggtttac
     2641 gcgcatcgcc aatgagctcc ttgaagctgt catgcgtgcg ggtctgtcgc agcatcagct
     2701 tttggtgttc atggctgtca tgcgcaaaac atacggcttc aacaagaaat ctgactgggt
     2761 cagtaacgag cagctctcgg agctgaccgg cattctcccg cataagtgct cagctgcaaa
     2821 aagcgtcctg gttaagcggg ggatattaac tcaaaccggt cgtgttatcg ggattaataa
     2881 aacggtcagc gaatggtcat ctttacccgt aaaaggtaaa gaaaaaaaac cttacctgaa
     2941 aaaggtaaca ttacccgaat caggtaagaa aagtttaccc gaatcaggta acgcctatta
     3001 cccgaatcag gtaaacacaa aagacaaaca tacaaaagac aataaagaca atattaataa
     3061 cccccctaaa tccccccggg cggtttcgtt cgatgcgtta gctgttcagt tgcctgactg
     3121 gctttctgca gaaatctggt cgtcatgggt ggcatatcgt cgcgacctga aaaagccgat
     3181 caagtctcag cagacggtca cccaggctat caacctgctg gaccgctgca gactgaacgg
     3241 ttacgcgccc gaagaaatta tcaaccgcag catcgccaat ggctggcaag gcctgtttga
     3301 gccaaatggt gccaagcctc aaccaagtca acaggtgcga gttgccgaaa atttcgcagg
     3361 gaaggattac gggcagactg aaatcccatc atgggcgagg gactgatcat ggaactggaa
     3421 gaaaaaatca ctgccattga gcggatgctt gatcagctga gtaagccacc ggaagacatc
     3481 ccgaattgcg aggtggttat cgagcgcgtc tgttgtgaaa agcatggcga gtatgagcag
     3541 cgcaagcgga tcctgaccag cagcatcatc aatctgccat caccgccgac acgctgcccg
     3601 ggctgcctgg aagacgaact gaattttctg aaggatgaaa aggttcgctg ggataagcga
     3661 gttcgccagc aaactgcaga aaggctgctt cgccagctgg atataccaga gcgattctcc
     3721 acgtgcactc tggacagcta caagcctgtt gggaaggatt ctgagcgagc attacgggtc
     3781 tgccaggcct acgcatcgaa atggactgat cgcctccagc agggaggtgg gttggttatg
     3841 tgtggcaagc ctggtaccgg taaaaaccac cttgcgctgg ccattgcccg ccatgtgatt
     3901 gagcaccacc aaagctcagt cattttcacg acggcgctca agattgcccg ggagtttaaa
     3961 tcgacctggt caaaaacagc cacgcgcact gaggatgagg tgatcagcta cttcacgaag
     4021 ccagaccttc tgattgtcga tgaggttggt gtgcagtttg gcagcgaagc cgagaagatg
     4081 atcatgtttg aaatcatcaa cacccgctac gagcgcctga agccgacgat cctgatcagc
     4141 aacctgccga aggatgagct gacgcagttt atcggcgagc gcgtcatcga ccgcatgaac
     4201 gacggcggag gctgcacgat ttcgtttacc tgggacagct atcgggagaa caggtcatga
     4261 caggcaaaga cgcaattctg aactacctga aaacgcataa aacctgtagc tctccagatg
     4321 tcgccgcggc ttccgggatg acgcatacct gcatcaacca ggctgcaaat atcctggcaa
     4381 agcaaggggt gctggtagct gaagctcggg tgtggcggac ggtttactac cggctggcta
     4441 ctgaagaaga aatttcaggc aggaagagca ccaatcagat tttcaacgag tgtcggcaaa
     4501 gcccggcgat gaaacgggta ctggctgttt acgggagaac atcagcatga ctatcacact
     4561 acaggcagta aacgagctca tcgccaccct ggagagcgca ggcgagctgt cgatcagaga
     4621 gcagaagttc ctgaagctgg cgaaagcgta ccagcagctg gctgcggaga atgtggaggc
     4681 aaaaaaaata atcagcgaat gccgggagta tttcatcgct ggggtgatga accgtatcag
     4741 accaacgaat gaaggatacc tgcatatgat ttgcgacacg tttgcagacg aaaccccagc
     4801 aaccgatcgc atcgtagccg ggattaaggc tgatggggtg gatgagttcg tatccaacgc
     4861 cgtgcataag atttttgatg aaagcggagc agtgtcagct ttggcttacc tttcgctcgc
     4921 taattcatac gtgaagcaac tgcgcgaggg ggcgacaaat gaccaagtca accataacca
     4981 gagatgagct taacgaagtg attgccacat acggaaagca ccacattgct catcgaatgg
     5041 ctaatgtatt gttggccgca atggacagcg agccagattg caaggaaaga aaacttttct
     5101 gttcaaccga tacgaccaga atgagaaaga caatctctgt ctctgctggg accgaggatg
     5161 cgccgctata tcgccacgcg cagcagccgg taaatagcga gccagtagga taccttttcc
     5221 ataacgaata cggtgtggtt ttatactcaa tttccgatga tgctaccgag ggattctcgc
     5281 taattgggcc aatatactcc gtaccgcagc cagcgccggt agtgcctggt aaatggattc
     5341 cggtaagcga gcagatgccg gaagaccgca cgcaggtaat tctatgggat gctgaaattg
     5401 gagaagtaac aagcggtcac tacagccata aaacacatac tttttatcat tgcggcgatg
     5461 ccatcgaaaa cgagataacc cactggatgc cgacgccagc agctccgcag gagatgaaag
     5521 gtgaataaag ccgaactatt gcagaagata tcagcgctcg ctactgaatg ccacacgctg
     5581 gcctgtgatc ttgatattgg tgatgagcga accgaaatgt tcgaaatcta cggcgtgctg
     5641 cacaatctcg gtcgccgcgg gtacgcctgc caggtagggc ggcgcatgaa tccactgctc
     5701 gcatcctgcg atgacgacga ggatgaggaa gatgacgatt gggatgagga tgacgactga
     5761 tgcctaaatc ccctgcagaa cgcaaagccg cgcagctaat ctgttagtga tatataatcc
     5821 cctccacagc agaggggatt tttatgacaa acaaaaaaat gacacctgcc gaaaagctca
     5881 aagcatcgcg gaagcggtat aaaaaaatct ggcttcaaat ggatatcgct aacgccaagc
     5941 gatttggcga gaaagaagtg ctttccgttg acacctacag atcgccctat gaaacgcgca
     6001 agaaaagagg gaggacagca gattgacaac atcatcctgg aacatagcag ccaaatcgaa
     6061 agacgagcag gacaaagtca acgttgacct cgccgcgtcc ggcgtcgctt acaaagaacg
     6121 cctgaacatg ccggttgtcg ccgaagtggt ggccagacaa cagcctgaac acctgcggga
     6181 ctacttcatg gagcgcgtcc gctactaccg cgagcagagc atccagcttc cccgcgcatc
     6241 cgatccgcgc tatctggaaa tggctgagca gaacgccaag aaatagcgat tttctcgtat
     6301 atgctcattt tgcttttatc cccgcgacgg gcgataatta cttagtcagt ctggacaact
     6361 gacaacttta ccccggcgcc aagtggggac acatggcgca aacactgcaa tttgagaaga
     6421 gttatcaaaa cgtactgatt cccgcagagc cgggaaccag cgaatacctg caacttatcc
     6481 ccgtagggca gctgctttgc ggtgagttcc gcaagccccg gaattacgca ttccacaaaa
     6541 agttcttcaa gcttctgact ctcgggtatc actactggac cccttccggt ggtctcattg
     6601 agcccgcgga gcgtaccctc atatccgggt ttatcgactt tctctcatcc gacctcgatc
     6661 agcgcgctgc actccagaac gccgcggaga tgcatctctc ctcggtcggt atctcccgtt
     6721 cccgcgatat ggcgctgctg aaacacttcg aatccttccg cgagtgggca accattcagg
     6781 ctggctttta tgacgaatac cagatgcctg acggcagccg tcgtcgtgtc gcaaagtcga
     6841 tatccttcgc cagcatggac gacagccagt ttaacggcgt ctacaaatca gtgctgaatg
     6901 tgctctggaa ctacattctg cgtcgcaaat tccactcgcc ggctgaggct gaaaacgccg
     6961 ccagtcagct gctgagcttt gcggggtgat ggcgatgaaa tactcatggt tccagcatcc
     7021 cgactgcaca accgagcagg ccgaacagtt agtgtccaga tatcaggcgc gaggcattgt
     7081 cacagagaaa agccttaacg cggattatct gagttggacg gtcagcgccc ggctgccggt
     7141 ttgtgttcgc ccggagcata ctccgcgatc acttcgtcaa cgtatatggg ggtgagcatg
     7201 gccaatcttc gtaaagcagc tcgcggtcgt gaatgccagg ttcgcatccc gggcgtctgc
     7261 aacggcaacc ctgaaaccac ggtattggcc catatccgca ttgctggatt gtgcgggacg
     7321 gggattaagc cgcctgatct gctcgccgct atcgcctgtt catcctgtca cgatgaaata
     7381 gaccgccgca cgcgcctggt agatgcggag tatgcgaagg agtgcgcgct ggagggaatg
     7441 gccagaacgc aggttatctg gatgaaagag gggttgataa aagcatgaac caatatcgca
     7501 tttcattacc ctggccgcca agcaacaacc gctactaccg gcacaaccgg ggacgcacac
     7561 acatcagcgc ggaagggcag gcataccgcg acagcgtcgc cagaatcatc aaagactcga
     7621 tgcttgatat cggcctggcc acgccattga aaatccgtat tgagtgtcac atgccggatc
     7681 gccggcgccg tgacctggac aatctgcaaa aggcagcatt cgacgccctg acgaaatcgg
     7741 gtttctggct cgatgaccag caggttgact actacagcgt gaagagaatg cctgtcgtca
     7801 aaggtgggcg gcttgagcta accattaccg aaatggagtc cgcatgagcc gtgacgttat
     7861 cgaacgcatc cgcgaccgct ggcaaaagct tcgcctcctg cgtagccgcg gcaccgtgct
     7921 ggtcgactac aaaatattac gcaatttcgt ccgtatctat aagcgcctgg gagagacagc
     7981 atgaaactgg aattaaccaa cgaacagcac cagtggatag atcagtggct ccagctttgg
     8041 ggcgcatggt gccagacagg gaagatagac aaggcgatga taaatatgat tgccaagttc
     8101 atggccacgg ttgaaccgca agcaccatca aggcctgtat gcagcgatga tgatgggttg
     8161 ctgattgatg ccgtaattcg acattacctg aaaaacgtag atgagaacgc atggaaagtg
     8221 atttttgcct attacgtctg taactcaagc gagataagga tcgcttcatg gcagcatgct
     8281 gtgagcaaac ctcgcctgat gaagacccgc gccggaaacc agtataagca cccgagcatt
     8341 tcaaccatcc gccgggaagt taagcagatt atcaacgcgg cgctcttctg cctgtaccag
     8401 ccgctgcaaa atgcgtttaa cgatcgcgaa agcgtgagga aaattgcaaa aaatagtcat
     8461 aacgtgcttg catttcaatg aacaaatgag caatatattt agtgtaggtt gccgtatttg
     8521 cgtttgacct atcagaacac cgagcctcgc catcgtgcgg ggctttttca tttcagggtc
     8581 agaagcacag cggttgtgcg ttcggctgtt aaccgaatgg tcgaaggttc gaatccttcc
     8641 tgtcccgcca aattcgccgg tctagttcag tggcagaggt ggtaaatgcc gagcaaagat
     8701 tactatctta atcgaagggc taggcttgct aaggccgtgg aaaagttggg tggtcgttgc
     8761 gcgagttgcg gatccgaata ttcgcttcag tttgaccaca tagacccttc caccaaatca
     8821 gcaaatgtaa gcgagatgca ctatcactca gattcggtgt tttatgctga ggttgaaaag
     8881 tgccaactac tatgttctgc atgccacatt cagaaaacca aatttgacct tagctactta
     8941 gtagctggtg agctgaatgg tatgagcaag ctaacaatgg acagcgtcca gttcatcaga
     9001 gaaaactaca ttccacgaca taaggtttat ggagccagag ggctagggag aatgtttggt
     9061 gtaacacatc aaacagtgct ttcagcctta aatggcgaaa cctggaaata agctgcgcgt
     9121 cagaggttcg attcctttgc ccgctccagt aaaagctttc cagtctgcga tgatgggttc
     9181 cccggagtga ctggaaagcg acctggtttt gaatgggcgc tgctttttgc aaaattgctg
     9241 tgtgaaaata ctgacctttg ggttcagcgc tcatccaaaa gcatcacgtg aattcaccaa
     9301 cgctcatgta ctctccagga aacaataagt gattctgaaa agttccggtt agatattgcc
     9361 ccggtcaccg gatgatttta tcttttggtt cgtggtgaat ccccctatgc ggcggggcgt
     9421 ccagtcaaac ttttttgtcc aggtttgttt gcgcggaact agtcggctgg ggctgttcca
     9481 ccgggaggca cccggcgcca cctccttgac ggtattgtta tttttcatgc ctgttcgtcc
     9541 gagcaggctt tttttgcccg cattatcttc attgaaaact ggcaactcag agaatcatcc
     9601 aaattaaaaa agcagcaacg agagcgatta ctaatacatt cttcgctttt tctcttctgc
     9661 tggccttcac aaaaggattc actatccggc agtggctaca cacaagtgat tctgggttaa
     9721 ttaactttcc gcagtagggg cagggcttga taatcattgg tactaattaa gttgtgagaa
     9781 cgtagtctca tttaaccaca tttatcgagt tgttttacta atggtctagt tatttcagac
     9841 atattcatca atttcaggct cacgggaatc atccgctacg tgctttgttg ataaatccag
     9901 cccgtgaagc ctgacccttt tcaaacacac acagcgccat ccgaaaaatc ggaggtgagg
     9961 ctatgaccag aatgagcacc atttacagca gactttcata tggaacagga accacgctga
    10021 ccggctgcgg tgtatcagcg aaggcatatg ccgaaacagc taaaacagca aaagaggtgt
    10081 cctggatgtt ggccgacaga attgcagggt taagcctgag cgactgggca attattgtcg
    10141 gtatcgcatg cactgttatt acctgtgcag tgaactggta tttccgctgg aaagaacggg
    10201 aggatcggcg caatggctat gccaccaaag ctgaagaata gcgttattgc agcgataccc
    10261 gctggcgcta ttgctatcgc tgcggcgttg attactggcc caacgggtaa tgatggcctt
    10321 gaaggtgtac gctatcagcc ttatcgggat gttgttggag tgtggactgt atgctggggc
    10381 catactggta aagatattat cctcggcaag acctacacca aagcagagtg ccaggcgctg
    10441 ctggataaag acctgaatat tgtcgcccga cagattaacc catatatcaa tgctccgatc
    10501 cccgaaacga tgagaggggc gctgtactca tttgcgtata acgtaggcgc gggaaacttc
    10561 aaaacctcca cactgttacg aaaaatcaac cagggtgatt cgaaaggcgc atgcgaacaa
    10621 ctgcggcgct ggatctatgc tggtgacaaa cagtggaagg gtttaatcac gaggcgtgag
    10681 attgagcgtg aagtttgcct gtgggcagag aaacctcagg ttctggggga tggactcggg
    10741 ccgcttaacc ctggcattcc ggtatcagtt ccgggggtat tctgatgaaa cccagaaaaa
    10801 ttacgattgt tgcggttctg ctggtggctg tcgtaatcat tattgcagtg cttagcgtat
    10861 tactggttcg tagccgctca gctcttgaaa cagaacagag tgagaatcgg gtattacgta
    10921 atgataacgc gctgcaggcg acggtgataa ctacacaggc tttcaacttc aatcggttta
    10981 accagatagc agagaacgcc aaccgcctta actcgctgat cgatgccggt actgagaaaa
    11041 ctgtcatcga ataccgggag attctccgac gtgaaaagac ctgtgatctg cctgttcctg
    11101 ctgatgtcgc tggtgggctg ctcaaatacg cgtaccgttt acgtgccagc gcaatgcacc
    11161 ccgataccgg gaacactaac gcaaccgatg atagtaccac tgccgccagc tcaatgacgt
    11221 attgtcaggc cgtcctctgg attgagccgc tactggcaac gatagaaaag ggtaataaca
    11281 atttttccgg tatacgggag attgagaata accgcgcaat gcgataacta tcacagtttc
    11341 actcggtggt tagcttgata aggataaaaa ggactccatg atgtagattc tgatgctaat
    11401 aaggataaaa aatgattttt cccattgtga agtctgtcta catcaacagc aaaactgata
    11461 gtcgtttaat acgttatgat gttattcgta ctgaccccga tacctacctc gtcaaagttt
    11521 tcgataatca acagcgtgga atttcggtac caggcctgat tagtcaaatt tccgagttcg
    11581 aaataactcg tgctgcctat aatgaaaaat atcaggtggg taatcgcgta gtcgtgaaaa
    11641 tgaatgcgga ccccggattt gaaaacacca ttgatggtga gttgcaagca catcgagata
    11701 gtttgtcgtg aaaacaaccg cctccgggcg gttttttgtt tctgcatggg gaagaaatta
    11761 tggctgctct taagaacctt attcagcagt tgaatcaaat gcaaaagcaa attccatttg
    11821 cgacagctca ggctatgacc aaagttgtac gccagataga gacggcacag aaaacagcgt
    11881 ttgaacgtca tctggagagc cctacaccgt ttactgttaa atcagttggt tcggttgctg
    11941 cgagaaagaa caacctgacc gcaaaggttt ttgtccgtga tactgcggct ggctatctgg
    12001 aaccattcga gtttggtgga gagcacaagc tcaatagcca ggctcttttg aaccccaaga
    12061 acgtcaaact gaacaaatac ggcaacatgc cgcgcaataa gctctcacag cttaaagcaa
    12121 aggaaaatgt attcgtaggt gaggtggatg gcattaacgc tgtctggcag cgtaagaagc
    12181 cgatgaaagc taagaagcga cgggccaagc gctccgctaa tgggacgcga aggccgaaac
    12241 gtaaacagcg ttctccaaag cttttgatcc ggtttggtga tgcgctacct gtgactccag
    12301 tgctggggta tatggatagg gcccgtacca tggcgaacgc actgctaccg tctgctttaa
    12361 atcaggcgat agcagaagcc atcaggacgg caaaataaaa gcagtaactt ataagctaat
    12421 ttcgcaagct tttatgaagc tgtttactgc agttgtcgac ccagaaacat tggctgacat
    12481 ggaatgctgg tttccgccat cctttgtttg cacaccaact aacactttgg atttcgcccc
    12541 ctgaagctgc ttaagcactg tttttagttg gtccgtgtca tcagattgaa tctggaggct
    12601 ctgaacatta cgtcttgaaa gggtagcatc gagcttcact gcggtattcc cgtcgacctt
    12661 cattatcagg tccattggta cctctgatag tgattcggtg cttttatcca tttcaacgta
    12721 tgccgccgat agcttttctt tagtgcagtc aaacacaatg gcgccattgt cggatgaaac
    12781 ctcgccaagc atcattgctt tcttaccacc agagaaaagg tcatcttcag tattagttac
    12841 ccactgggca tgagcaattg gtgatgccag cactgcggct acgaaagtta ttttgattat
    12901 attgttaccc attacattct ccttgtattg aataggaata atcatagtcg gagcgaatgg
    12961 tcgaagccat taaaaaatgg gtccttcctg agacttttgt aaggtacggg cattgcgcgc
    13021 cgcggtgttt tcctagctac aactttcaga tttgtgtccc atgtcccacc tctggcgatc
    13081 attacggaca cctcgccagc tctggctatt ccagtttatt ccagtgggac attctggtgg
    13141 gacatggcaa aaatgtccca ggcgaatgtc ccaccccaga aaatgtccca ggtgatgtcc
    13201 catgaccacg atgaaccaga gtcagtacgc acaacattca ggtgtggatc gcaaaacaat
    13261 tggtcggtgg attaaagccg ggcgcttcat tgtgatggac ggagacctga ttgacgtaga
    13321 ggccagcgat gcggcattga agaaaaaccg cgatggcaaa gacccgcgtg cctcgaacgc
    13381 gaagaaaaag aaaactcccg tcgttagcga taacgatgat gacggtgatg aaatcaataa
    13441 aactgtccgc cagataatgc tcactgaagg ggcagatctt tcgagagagg aagcgggacg
    13501 tatccgcgag aattacatgg ccctgcaggc aaagctgcag tatgaaaaag acagcggcca
    13561 gcttattgag ctgacagcag ccgaggaggt tttattcaac gcctttcgcc aacagcgtga
    13621 tgcctggctt aactggccgt ccagggtggc gccgctaatg gctgctgatc tggatgtacc
    13681 ggcggacagg atgacagagg tgctgattga acatgtccac aaacacatct cagtcctcgg
    13741 agagccagag tttaacccgg cagaagattg agcgtcttga attaagcgtc cgcaaaggct
    13801 ggacaccccc gccgcgtatc agtgtgccgc agtgggcaga tgactatcgt aagctggcaa
    13861 aagaggctgg gagcacttcg ggaaactggg aaacatcgac ggtagaaatt gcccgcggac
    13921 cgatgcttgc cgcgacggag tccggggttc atatcatcac tgtaatgtgc tgtacccagt
    13981 tgatgaagac agcactgctg gaaaaccttt ttggctattt tgcccacctc gatccttgtc
    14041 cgatactgct gctgcagccg aaagaagaag ccgctgaaca gttttcgaaa gagcgtatta
    14101 gcccgctggt aagggtgacg ccggtactgc gtaaaatcat cggtgattcg aaacagaaaa
    14161 gctcgaaaga aaccattctt tacaaggcat tcactggcgg atttctggcg ctggcgggtg
    14221 ctggtagccc tgataacctt gcgcgtcgtc cgattcgtgt cctgctggcg gatgaagtgg
    14281 acaagtaccc gataacccgc gaaggcgatc caattgcgct ggccgaagag cgtacagcga
    14341 catttggcct gacctggctg tctgtacgcg cctgttcgcc gacggtggag gatgagagcc
    14401 gcattgctga cagctacgcc gactccgatc agcgccgggc atctgtggtt tgcccgcact
    14461 gtggccaccg ccagttcccc gactttttca aacacgttca gtggccgaaa gagggagata
    14521 aacacctgac taaatcggcg atgctctatt gcgaatgctg tggtagtggc tggtccgaag
    14581 gacagcgcct cagagctctg cacactattc gatggcatca gacgcgccca tttgagtgct
    14641 gcggggagcg gcactcaccg ctgatggatt atgaccttgc ctggcgggcg gcagacgagg
    14701 gcagcgttga aaaggtctgg caatggtcag agtcggaacg gcatgcggtc tatcgcgcaa
    14761 tctgcccctc ctgtggaaag gaggcagtcg ataaccacca cgcggggtac caggcatcca
    14821 agcttttcag cccctggcaa aaagataagc cgtcggatat tgcgaaaaaa tatatcgatg
    14881 cgaagggcga tccggataag gaacaggcgt ggtggaatac ccagatgggg cttccgcacc
    14941 gacctaatca tgggaaacag ctccctgttg atgttctgct ggcgcgccgg gaaatatttc
    15001 cggccgtcgt tccggacggg gtggcattgt taacagctgg agttgatacc caggacgatc
    15061 gcttcgaaat tacgatcacc ggctggggga gagatgaaga atcgtggtcg gtcgcgcatg
    15121 acgttattta tggtgacctt gagacggaag aaccctggaa gcgactggat gcatacctga
    15181 aacagatctg gcgacgtggt gacgggcgcg gcctgaatat catggcaacg tgcatggact
    15241 ccggtggcca ccatacgcag aaggtatacg aattcgccaa agagcgtctt ggccgtcgtg
    15301 tctgggcaat taagggggag tctgcacagg gaggtaaacg caatcctgtc tggccgacca
    15361 aacgaccatc atcgaaaagc aaagccagtt tccgccctgt cattctgggg gttaactcag
    15421 cgaaagacgt gatacgcggt cgcctgcatc ttgagccacc caaacctggc gccgccgctg
    15481 cgggttatat gcattttcct gacgatcgcg atctcgggta cttcaatcag ctgctggcgg
    15541 agcgactggt ttacaaagtc atttccgggc agcggtacag tatctgggaa gcaataccag
    15601 gacgagctaa cgaagcgctt gactgcctcg tttacagcta tgccgcgctg tgcggtctca
    15661 aacatatggg gttaaaactc aacgtccggg ccgccaacct cgaagccgat ccggataagt
    15721 tcctgccagc gccagttgga caggaagaaa aaatcaatta cgagctgccg ggtgcggtta
    15781 ttgaagaacc agcgccggtc aaacgtaagc gaatatcgca actcctgccg aaataaggaa
    15841 aatcatgttc aaccggaaca ccagcctgct tgccggcgca atgactgacg atcagctcag
    15901 ggatgcgctt gcgaaagctc agcaggcgta cattgattta gcaaccggga gccacggtgt
    15961 ttcgttttcc tatacgcagg gagacgggac gcgatcagtg tcctatcagc aaagcaccct
    16021 ggctgatctg ctggccctga ttcaacttct gcaggcgcaa ctggggatta tctctcgtcc
    16081 ccggaaacca gcgaggttta gattctgatg aataaagtac agatactggg ctctgatggg
    16141 cagccgttgc gacagcagcg tccctctatg ctggtggggg ggagccgcgt accttatgac
    16201 gcagctgact ctttcagcga tcaactggcg aactggcaac ccgcgctgtg gtccccggac
    16261 aatgaaatta acatttaccg ggatcgcatc gtgtcccgcg cacgcgatct ggtccgtaat
    16321 gacggctggg caaacggtgc ggtcacacgt ctgctggata atgcggttgg tgccaacttc
    16381 cgccccatca tgaaacccga ttaccgtgtt ctcagaatga tcaccggaaa caaggcgttt
    16441 gatgcgtcct gggcggaaga gtacggaaaa gcactggacg ggcactggcg gacctggagt
    16501 aacgatcctg gccggtattg tgatgttgaa cgaaaactca ccgtgtcgca gatgttacgc
    16561 ctgggatttc gtcacaagct tattgacggg gatgctctgg ccattctcca gtacagaact
    16621 gacaggcttg gtcccggaag agggcgttac gccaccacgg tacagattgt cgatcctgac
    16681 cgcctcagta atcctcagca gaatttcgat atgccaaatg tccgtggtgg cgttgaaatt
    16741 gatgcggacg gtgcgccggt tgcttaccac atcagggagg cccatatcgg tgactggtgg
    16801 agcggggcta aaaccatgac gtggcagcgt atcccgcgtg aaactgactg gggccgcccg
    16861 catgtggttc acgattttga tcatgagcgt ggcgcgcagc accgcggtaa cggcatcctg
    16921 actccggtta ttcagcgtct gaaaatgctg gtgaagtatg accagagtga gcttgaggca
    16981 gcaattctta atgccatatt cgccgcttac attgagtcac cctatgaccc tgcgatggtt
    17041 cagtctgccc tgggcgagac ctatgacgag tcggagttag gcacttatca ggacgggcgt
    17101 gttgagttcc ataacgatcg gcgtctgaca cttcagaatg gtgcccgaat gcccattctt
    17161 tatcctggtg agaaaatcac gacggttaac gcggcgcggc cctacagcaa ttttgaagtc
    17221 ttcgaatctg ctgttctccg taatttttct tcaggaacag ggttgtcccc acagcaggtc
    17281 acccaggact ggtctgacgt taattacagt tctgcacgct cctcgttgct ggaggcatgg
    17341 aaaacactga ctcgccgccg ggacgatttt tctaccggct tcgctcagcc cattctcacc
    17401 gcctttgttg aagaagttca cgacaatgag gatttacccc tgcccgcagg cgcacctgat
    17461 tttgttgacg ccagagccgc gtattctcgc gcgcgctgga tggggccagg gcgcggctgg
    17521 gtggatccgg ttgcagagaa aaaaggcgcc attcttggtc tggatgccgg actttccacc
    17581 ctcgagattg aggtgggtga aaacgtcggt gaagactggg aagaagtgct tgatcagcgc
    17641 cagagagaaa ttgagtcatg tcttaaacgc ggattaccgc ttccgagctg ggcacaggct
    17701 gaccagtttg cgagccagac cattaccgat ccggaggaaa agtgaatcta ccccatctgg
    17761 cccagcgatt atttaacacc ccgctggcgc tgcacccgag taaagccgaa gtcatcatgg
    17821 catccgtaat ggaccgattt ggtatcagta aaatcgaatc ttctcttgcc atggaggatg
    17881 actggtacgg atatgacgat aaccggggac gtgaatcccg tagtgatccg ggttatgaca
    17941 atgtgctggg tgtcgccgtc atcccgatat gcggaacgct ggtgcaaaaa ctgggcagtc
    18001 tgcgtccgta cagtggaatg acagggtatg acggcattcg tcaggcgttt cttactgcga
    18061 tggaagatcc cgacatttcg ggcatttgcc tggatatcga ctcacctggc ggcgaggtcg
    18121 ctggatgctt cgatctggtt gatgtcattt acggctcccg ggggaaaaag cctatccatg
    18181 ccattctgac ggaaagcgct tattccgctg cgtatgccat tgccagtgca gcggaccgga
    18241 tttctgttcc gcgcaccggc ggagtgggtt ctgtgggtgt gatcaccatg caccttgact
    18301 ggacgcagcg gattaaagat gacggtctta aagttacgat catcacctat ggatcccgca
    18361 aggctgaagg ttcgccgctg agagagttgt cagatgaagc gctggccgcc atccagcagg
    18421 acattaacac catgggcgaa ttgtttgtga acactgttgc cagaaaccgg gggattagcg
    18481 caaaggttat aaaaagtacc caggccgcct gttttatggc tgctgatggc gttgagattg
    18541 gactggctga tgaggtgtgt cctcctgacg ctgcgttcaa aaacttactt gaaaaaacag
    18601 gagcctgaaa tggcaaagaa aaagacgttt agttttgctc acctcattgg tcttggccct
    18661 tccgcttctg aggaagaaga ggataaaaaa gccaaaaaag cgaaagcccg tcgcgcggaa
    18721 gaggacgagc gcgaagatga tgccgatgat gatgagcgcg acgacgacgc ggaagaagac
    18781 gaacgcgacg atgatgctga agatgacggc gatgatccgg atgcgtcaga agatgatgat
    18841 tctgaagacg acggcgacga cgatcgcaaa gagagtaagg cggtaaaaaa tgcacgcgct
    18901 gctgagcgta aacgctgcgc ccgtattttc ggcagtaagc atgcagctgc gaatccttca
    18961 ctggccgcgt cactggcttt caataccggg atgagttctg cggcagcaat taatgtccta
    19021 gcctcttcgg ctccggccgc agccgcatct cagccatccc gtaaacgctc tctcgatcag
    19081 cgtatgcagg aaagccacca ggtccggctt aatccggata gcggacagaa agagaccgga
    19141 aagtctgcgc tggtaagtaa aatgaccggc ctctacaact ccacaagagg agagaaataa
    19201 tggatcagtt tggtcagaat gcgtttgcgc ctggcatgaa gagcgcgctg tttgttccgg
    19261 atcagcttgt cgctggcacg ctccagctgg tgactgacac cgggatcatt acgggcggtg
    19321 cctttaagcg tggtactgtc ctgggcctgg tggctgccag cgggaaatac acgcaatgtg
    19381 tgaaaacggc tgaagatggc agtcagttac ccgttgctat tctggttgat gatgttgatg
    19441 catcgtcttc cgatcagaac ggcggcctgt atctgatggg ggaattcaac cagcaccgaa
    19501 ttatttttga taactcctgg acgatcgctg acctgaaaaa agcgctccga ccgctggcta
    19561 tcttcctgaa agacagtgac caggcacctg taaccacctc ctgatttccc ccacggctct
    19621 cctgacgaat gctttaaccg gcaggggctg gctcgtttaa attttttgcc agctgcggct
    19681 ggcactatca agagactgaa tatggaaaat atttttgata ccagcgtgct ggtgcaggtt
    19741 gttcctaacc tgaaaaccag tcagaactgg ctgctcgatc gcttcttccc gaatgtcgtg
    19801 acttacgaga ctgaagaagt ggcgattgat gttgatgtcg gcctgcgtcg tatggcgccg
    19861 ttcgtctccc cgctggtgga aggtaagctg gtcgagtccc gtaaatacca gaccaatacg
    19921 ttcaaaccag cttatatcaa agataagcgc gcgccggatc tgcgtaaacc gattcgtcgc
    19981 cagattggtg agcgtattgg cggtgaatat accgccgcag aacgcgaaat gttgaatctg
    20041 cagtttgaga tgaccgatca gattgacatg atcaaccgtc gtcttgagtg gatggcggcc
    20101 agcgcgctgg tgtccggtac cgtcacggtt gccggggaag gctatgaaac caaagtagtg
    20161 gatttcgggc gctctccgga tttgactatc actctgagtg gttcagataa gtggccgctg
    20221 acggttgccg cgggtgccac taatacccag ccctctgatg atattgaaat ctggcagacg
    20281 cttttcctga aagaatccgg ttccgtcgcg acggatctgg tgttcacaag caagtcatgg
    20341 cgtgctttcc gactggacac caccatcaaa gataacgcca tcacgttccc ggcgctgagc
    20401 ccgtttggta accagattaa cgctggccca caggcgatga agggcgctat ttataaagga
    20461 cgctggggta actttgacct ctggttatat aacgactggt ttattgaccc acttgataat
    20521 gtcgagaagc cgatgatccc cgacggcgcc gttattatgt ctggcgctga tctgatgggt
    20581 acccgcgctt ttggcgttat cctggacccg gcatttaact acggtcccct ggcctatgcg
    20641 ccaaaatcct gggtgaaaga agatccggcc cagcgtctta tcctgatgca atcctctccg
    20701 ctggttattc cgagccgggt aaatgcatcc ctttgtgcaa cggtggtctg atatggctaa
    20761 aacaaccaaa actgtactgg gcgatgatct gaatgcggaa ggcgccaccg aagacgccct
    20821 gaatatcgac gacctgaatg ctggtggcag cgttcatgag acccaacagc atgacgataa
    20881 acacggcgaa ccatcagatg atgaggatac cgctgaagaa gatgaccagg aagatgcccc
    20941 ggagcctgag tttgtggtgc tgaaagggaa ttgcatccgc catgacggtg aggtctatcg
    21001 ggaaaactcc cttattccgg tctccggtaa ggatgccgag cgtctgctgg cagcaggtgt
    21061 aattgccgat gtccatgctc tgcgacagcg tgcattatct gctgtgcgtg gtgtgaaaat
    21121 cacaacggag taagcagatg ggcgtggact gggatttaca tcttctgagt ccgctccatg
    21181 gcgtgtttgg cgatgagcat gagtaccgtc cccgcaacgg tactcctttt acgattaacg
    21241 gtatttttga ccgtggctat gcgcagattg cagaaaatct tgatggtgaa tcagaaatta
    21301 acacctccag cccgatgctg ggtgtgcgtg atgctgaatt tcgtcaattg ggtaaaccgc
    21361 agcctgccgt atctgaccgt gtgtttatta aaacggtcgg gggtcaggtc atcaatcagt
    21421 tatttgttgt gtccaacgtc gaaccggaca gccatggcgg ctcgcgtctc gttctcaatg
    21481 tagcgaaaac ccgatgaatg cttccgcaat acgacaaatg gtcgtggccg cgctgaaaga
    21541 taaaaccgcc gccggcgatc gtgtgtattc cccgcgtgac tgggcaactt cgccggactt
    21601 gtaccctgca ttgcttgttc agacgccatt tgatcacaaa aaggcgcagg ggcgaaatac
    21661 cccggccttc acctctctga ccaccgttcg tattaccggc cgggttcagg agtatgacag
    21721 tgaaaccact gatgatggcg ccatgcgcgc ggaggttgca ctggaggagc tccgggaaca
    21781 ggtggaaagg gcggtgatta acagctacga gttgacccgg aacatccaga aatatgcgga
    21841 ggttcgctcg acgattgatg ttgatgcgga tggcgaagcg catatgggcc agctgcttat
    21901 cgaaattgat attgagcact atcagggacc ggaagacttt tatccgatcg atacggtacc
    21961 gctggcgggg atcgacatca ccatcaacat gccggacggt acgccgcagc cgggcgtaaa
    22021 aatagacctt caggagtaat catgtttgta aaaccgaagg acgggatcag cgttcgctgc
    22081 cctgtcaggg gcatcccatt gcctaaagag ggtgctgaag tacctgacaa tattttctgg
    22141 cgtcgccgtc tgagtgatgg ggacgtgatc ctctctaaaa aggatgaggg cgcgccagag
    22201 aaacaatcat tacctaaaaa agcgggagaa aatgaatgac cgtacctttc gctcgtgttc
    22261 ccgataacct gcgggtaggg cttttcttcg ttgagtttga taactcaatg gcgaataacg
    22321 ccactgccac gcagcgcacc ctgcttatcg gtgggatgct cagtaccggc tcaacccccc
    22381 ctggtattcc gcagcgagtt tcctcttcgg ataccgtcgg tgagctgaca ggaaaagggg
    22441 gaattctgca ggccatgatg gcggcgtatc agaaaaatga taccgcagcc gaagtctgga
    22501 tcctgccgct ggaggaagac tccgattcca tggtggctgc aaccggcacc attaaagtga
    22561 gcagcgcacc gacggcaacc ggagtgatct ccctttatat tgctggtgag cgcattcagt
    22621 tgaccgttgt agcaacagat acggtggcag cgatcgccac ctctctggcc gcggcgatta
    22681 acgcaaaaac cacgctacct gtaaccgcca gtgcgactac ggataccgta accctgaccg
    22741 cgaagaatct tggtgctacg ggtaatggga tcgacattcg cctgaacttc ctcggcttac
    22801 ctggaggcga gtccacacct gcaggcctgg aactgacgat tactgctatg tctaacggag
    22861 tcggggctcc ggatattacc ggcgcgctgg caaacctgca ggatcggaca ttcgatttca
    22921 tcatcaaccc ttacgacgat acaacctcgt tgaatgtgat gaaggagttc ctgtcagaca
    22981 ctggcggtcg ctgggcatgg gacaagcagc tttatggcca ttcctttggt accaccaccg
    23041 ggacttacgc ccagctcggt accaaaggtg agctgcgcaa taaccagcat gagaccctgc
    23101 tgggcgtaaa taaatcgccg tccccttcct gggcatggtc tgcagcttac accggcgcag
    23161 ctgcggtgag tctgcgtaat gaccccggcc gcccgctaca gtcgctcgct gttcaggggg
    23221 tgcttgcgcc agaactgcag gatcgctttg agctgaccga gcgtaacaat ctgctgtaca
    23281 gcggcatttc gacatttacg gtcgatgacg atggcacggt gcgcattgaa aacctgatca
    23341 ccacctacca gaaaaacagc tatggcgatg cagatgacag ttatctggaa gtggagacgc
    23401 tgttcagcct gatgtttgtg acccgctacc tgcgcacagc ggtgaccagc aagtttggcc
    23461 gtatgaagct tgctgcggat ggaacccgat ttgcacctgg cgcggcgatc gtcacgccaa
    23521 acattatcaa ggccgatcag attgccgagt accagactct ggtatggaac ggttatgcgc
    23581 aggatgcgga ggcattcgca aaaaatatca tcgtcgagca gaacgccaaa aatccgaacc
    23641 gcgtcgatgt gctgtggccg ggaaccctca tgaaccagtt gcgcattttc gcgctgctca
    23701 atcagttccg cactcgggct gaatcaacag gagcttaaac gatggcaggt gatactacta
    23761 accgcctggc gggaaccgcc tatgtcactg ttaacggtgt gacggtaatg gtggagggct
    23821 cgtttaaata ccaggctgcc accgtaaacc gtaccaccct gacagggatg gatggtgtgc
    23881 acggatataa ggaaaaacct gtggcgccat acatttctgc ccgactgcgt gacagtggcg
    23941 gaacgaatgt gcagggcttt aaccagcaga cgaacgtcaa cgtgatcgcc gagctggcta
    24001 acgggaaaac tatcattggc cgttcactct ggacggtcaa cgtccaggaa gtggaaagcg
    24061 aagatgcagt atttgatgtt cgctgggaag gccgcgacgt aacggagaac taagatggct
    24121 gagattgaac gcgttaaaac cattccatta accgtagcgc tggatgatgc tgcggagaag
    24181 accacttata cgcagctgga gctgaaagca cccacgctaa gccaggctga gcagttttac
    24241 gagaaacagg ctgcgtcaac gtcgctcgcg gcgatgcgcc tgcttattgc gctggtttcc
    24301 ggtacgcgtg aaagcgtact gcagccgatg gattttctcg acttccgtaa gtgtgaggag
    24361 tatctgctca gttttttgac ctggaagccc tgacaacctg gcaggaaatg gccgctgacg
    24421 tcaccttcta tttccgctgg tctgaggaca gggcgtgggg aatgacccgc gcccggctga
    24481 aatggtgggt ggcgcaggca tcccggataa acaagcttag gaaacctgaa gacgatgagt
    24541 aattcttttg attttgagct ggtggccagc gaccaggtta gcgaggctat agaccgcatt
    24601 aatgaggctg tccgtgacct ggagccgaag ctagataaaa ctaaagaagg gctcaagtta
    24661 ggcggtcagg aaacagccga cggactgagc ggttttattt ctcgcctcga gaatatgtcg
    24721 aagagcgcgc gggataacgt gcagtttatt ggcgacatgg ttcccccact gaaaatggtg
    24781 ggggagctca cgg
//
LOCUS       DAJSKJ010000046        24694 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000046 DAJSKJ010000000
VERSION     DAJSKJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.046"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            144..1724
                     /locus_tag="ON345_001593"
     CDS             144..1724
                     /locus_tag="ON345_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR3071673.1"
                     /translation="MEWIADPSIWAGLVTLVVIELVLGIDNLVFIAILAEKLPPTQRD
                     RARVTGLILAMLMRLLLLASISWLVTLTQPLFSIRDFTFSARDLIMLVGGFFLLFKAT
                     VELNERLEGKDSDNPTQRRGARFWGVVAQIVVLDAVFSLDSVITAVGMVDHLAVMMAA
                     VIIAISLMLLASKALTRFVNSHPTIVILCLSFLLMIGFSLVAEGFGLVIPKGYLYAAI
                     GFSVMIEFLNQLAIFNRRRFLTANQSLRSRTTEAVMRLLSGKKEDAELDVGTASLLVD
                     QDDQQIFDPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLSAPESEIRALLEKNQHT
                     RLVVTGGDDEEDLLGVVHVIDLLQQSLRGEPLDLRVLIRQPLVFPEALPLLSALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEAEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEHLQRIPTAGEEVQIGPWTLKTLQVESHR
                     VQKVQIIPPPQEEWDYEV"
     gene            complement(1764..3608)
                     /gene="asmA"
                     /locus_tag="ON345_001594"
     CDS             complement(1764..3608)
                     /gene="asmA"
                     /locus_tag="ON345_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR3071674.1"
                     /translation="MRRILTTLMILLVVLVAGLSSLVLLVNPNDFRSYMIKQVAERSG
                     YQLELDGSLRWHVWPRLSILSGRMTLTAPGASAPLVRADNMRLDVALLPLLSHQLQVE
                     QVMLKGAVVQLTPETEAIRDQRAPVAPKGTSLPQESGDRGWSFDIAGLQVIDSVLVFQ
                     HESDEQMTVRDIHLQMQQDAGHQATVDFSGRMNRDQRDLSLRFSAAVQAGNYPQSLDA
                     QLSQISWQLQGADLPAQGINGNGSVNVSWQEDAKTLSFNALNLMANDSTLTGKGSVVL
                     GDKPVWSLNLQSDKLNLDNLLVRSDILLGGGAQQAQSAPNKPRPVIANGVNLPPYSGL
                     KSFDGSLAMTANQIVWRGLPFTHVTLQADNQRGLLHISQLEGSLAGGQLSLPGTLDAR
                     SGTPQAEFQPKLDNIEIGSLLKAFNYPINMTGKLSLAGDFSGSVIDANHFRRDWQGQA
                     NLELRDTRTEGLNFQQLVQQAVERSTDVQAQENYDSATRLDELTTDVDLDSGILALSN
                     MKGKSSLMQMTGKGTLDLMKEEGDLQFGIQVTDGWQGQGKLVDILKSTAIPLRVYGKW
                     EALNYSLQVDQILRRQLQNEAKRRLNDWAERNKNTQSGKDVKQLLDKQ"
     gene            complement(3635..4216)
                     /gene="dcd"
                     /locus_tag="ON345_001595"
     CDS             complement(3635..4216)
                     /gene="dcd"
                     /locus_tag="ON345_001595"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR3071675.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPIERINGATVDVRLGNKFRTF
                     SGHTAAYIDLSGPKAEVSAALNRVMSEEIVLAEGDAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRDQQGAVASRIDKD"
     gene            complement(4294..4935)
                     /gene="udk"
                     /locus_tag="ON345_001596"
     CDS             complement(4294..4935)
                     /gene="udk"
                     /locus_tag="ON345_001596"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR3071676.1"
                     /translation="MTDKSHQCVIVGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDSYYKDQSHLSMEDRVKTNYDHPNAMDHSLLFQHLQTLKRGNPIELPVYSYVEHTR
                     MQETIHIEPKKVIILEGILLLTDARLREEMNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5222..6934
                     /locus_tag="ON345_001597"
     CDS             5222..6934
                     /locus_tag="ON345_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCR3071677.1"
                     /translation="MLLHNHGLIQHGRSSVASGKTVVMKNNISVNPLRPSSQQHSLQI
                     LFGLWAATFLFTLFSLSLMHTHNVLSPVWFPTAIMTVAFYRVKRSLWPCMSVAGVLGI
                     VLATELYAPEQNGLLHPLTNLMEALVGALLLRRWLNLDNPLRNLRHWTQMAIATALIP
                     PLLSGIVVASLTPGGNAFQTLLVWMLSDASGALALVPLGMLIRADYFQRYRNRTLLIS
                     TILTAAITLALSVIAMLYMPWPFTFIMVLMMWSAVRLPRIEAFLIFLLIIMMVLTLSA
                     QHPLVLENIENTLNNYRLSNMLGLPFLMVQLPTGVMSIAMYAVYSERRRIAESETRFR
                     NAMEYSAVGMAIVDAYGQWLQVNKALSKFLGYPPDELHQMTFQQVTWPEDLAQDLEQL
                     DRLTRGEIESYSLEKRYRTRQGEAVWALLAVSLVRNADGTPLYFIQQIEDINDLKKSR
                     EANKRLMAEITETNEALFQEKERLHITLDSIREAVISADSQQNIIFMNPVAEKISGWK
                     QAEAQGKSLREVLYITQGDQGPRLENLYSDENLDSDIELNKVLHSRYGGSFDIHHSMT
                     PLST"
     gene            6956..7561
                     /locus_tag="ON345_001598"
     CDS             6956..7561
                     /locus_tag="ON345_001598"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR3071678.1"
                     /translation="MLVIQDVTESRRLLRQLSYSASHDALTGLPNRSSFESQLKGLLK
                     SLPGGHQSHALVMLDLDFFKAVNDSAGHAAGDALLREIAGLMRSLLRPGDILARLGGD
                     EFGFILRHCSTEQALSASERIVDAIKHYEFFWAGRLHRIGASAGLTLIHNHNAQLAEV
                     LVQADAACYTSKNNGRGIVTVYTPVHSSGDQSSACQICNNA"
     gene            complement(7498..8355)
                     /gene="alkA"
                     /locus_tag="ON345_001599"
     CDS             complement(7498..8355)
                     /gene="alkA"
                     /locus_tag="ON345_001599"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR3071679.1"
                     /translation="MFELPWLPPYDWRWMFGFLQARAVAGIECFHDQSYTRSWGLGPH
                     HGLITMTPDPARQVLRVTLSAGLLPVADEALMRVAKLLDLHCDPQQISRALGTLAAAK
                     PGLRLPGALDAFEQGVRAILGQLVSVTMAAKLTGKVVALYGEPLAQDASFSLFPTPER
                     LAAADPAALKALGMPLKRAEALIHFAQRAHSGELPQTQPDDVDQALKALQQYPGIGRW
                     TANYFALRAWQAKDIFLPDDYLIKQRFPGMTPAQIRRYAERWQPWRSYALLHIWHADD
                     WSPEECTGV"
     gene            8504..9856
                     /gene="yegD"
                     /locus_tag="ON345_001600"
     CDS             8504..9856
                     /gene="yegD"
                     /locus_tag="ON345_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004864597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR3071680.1"
                     /translation="MFIGFDYGTANCSVAVMQDGAPRMLMMENGSSLLPSMLSAPTRE
                     AVSEWLYRHHQVPATGDETQALLRRAMNYNRDEDIEVLGNSVQFGLASLKQYIDDPEE
                     VYFVKSPKSFLGASGLKPQQVAVFEDLVCSMMLHIRQQAQSQLQETIEQAVIGRPINF
                     QGLGSDEANTQAVGILQRAAQRAGFRDVVFQYEPVAAGLDFEASLSEEKRVLVVDIGG
                     GTTDCSVLLMGPQWRTRHDREQSLLGHSGCRVGGNDLDIALAFKSLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQTDFYSSANGRLLRDLARDARDAEKVSLLYKVWQQRLS
                     YRLVRSAEESKIALSESAQIDTHLPFISDTLATAISQDGLEAALNQPLTRILEQVQLA
                     LENSSEKPDVIYLTGGSARSPLIKKALAEQLPGIPVAGGDDFGSVTAGLARWAQVVFA
                     "
     gene            complement(9939..9998)
                     /locus_tag="ON345_001601"
     CDS             complement(9939..9998)
                     /locus_tag="ON345_001601"
                     /inference="COORDINATES: protein motif:HMM:NF025322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR3071681.1"
                     /translation="MMRLVIILVILLVISLPAY"
     gene            10251..11504
                     /locus_tag="ON345_001602"
     CDS             10251..11504
                     /locus_tag="ON345_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HCR3071682.1"
                     /translation="MKGSHKIRWRVALAVLVVVAGAAGIWHFSSNSDTSGSTAAKPVG
                     APSAGGGRHGGRFGGALAPVQAATATSEAVPRYLSGLGTITAANTVTVRSRVDGQLMA
                     IHFQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTN
                     LVSRQELDTQQSLVSETEGTIKADQAAVASAQLQLDWSRITAPIDGRVGLKQVDIGNQ
                     ISSGDTTGIVVLTQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEG
                     TLLSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMG
                     NEGHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEV
                     VEAQSATTPEEKATSREYAKKGARS"
     gene            11504..14626
                     /gene="mdtB"
                     /locus_tag="ON345_001603"
     CDS             11504..14626
                     /gene="mdtB"
                     /locus_tag="ON345_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR3071683.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEYMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNVQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVSDVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSVSRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQSIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            14627..17704
                     /gene="mdtC"
                     /locus_tag="ON345_001604"
     CDS             14627..17704
                     /gene="mdtC"
                     /locus_tag="ON345_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR3071684.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            17705..19120
                     /locus_tag="ON345_001605"
     CDS             17705..19120
                     /locus_tag="ON345_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3071685.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQDVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            19117..20520
                     /gene="baeS"
                     /locus_tag="ON345_001606"
     CDS             19117..20520
                     /gene="baeS"
                     /locus_tag="ON345_001606"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR3071686.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            20517..21239
                     /gene="baeR"
                     /locus_tag="ON345_001607"
     CDS             20517..21239
                     /gene="baeR"
                     /locus_tag="ON345_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR3071687.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            21430..21762
                     /locus_tag="ON345_001608"
     CDS             21430..21762
                     /locus_tag="ON345_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR3071688.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            21972..22268
                     /locus_tag="ON345_001609"
     CDS             21972..22268
                     /locus_tag="ON345_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HCR3071689.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            22270..22566
                     /locus_tag="ON345_001610"
     CDS             22270..22566
                     /locus_tag="ON345_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3071690.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            22669..24030
                     /gene="yegQ"
                     /locus_tag="ON345_001611"
     CDS             22669..24030
                     /gene="yegQ"
                     /locus_tag="ON345_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR3071691.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24360..24677)
                     /locus_tag="ON345_001612"
     CDS             complement(24360..24677)
                     /locus_tag="ON345_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071692.1"
                     /translation="MKKIAAISLTSIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         5442 a         6834 c         6756 g         5662 t
ORIGIN      
        1 catcaaatat ttcacgggaa tattatcccc tattttcaca atatccttag attttgtcat
       61 gataaatcac aggcagacgt tgcattttgc cgcggcttta tccatgatca atttgtcttg
      121 taacataagg ttttaaccaa cgtatggaat ggattgccga tccctctatt tgggcgggac
      181 tcgtgacgct ggtcgtcatt gagctggttc tcggaattga taatctggtg tttatcgcca
      241 tcttggcgga aaagctgccg ccaacccagc gcgaccgcgc gcgcgtcacc gggcttattc
      301 tggccatgct gatgcgcctt ctgctgctgg cctctatttc gtggctggtc acgctcaccc
      361 agcctttatt cagcatccgt gatttcacct tcagcgcccg cgacctcatt atgctggtcg
      421 gcggcttctt cctgctcttt aaagccacgg tagagctcaa cgagcggctg gaagggaaag
      481 acagcgataa ccctacccag cggcgcggcg cgcgcttctg gggcgtagtg gcgcagattg
      541 tggtgctgga tgccgttttc tcgctcgact cggtgattac tgccgtcggc atggtcgacc
      601 atctggcggt gatgatggcg gcggtgatta ttgccatcag cctgatgctg ctggccagta
      661 aagcgctcac ccgctttgtg aacagccacc cgaccattgt tatcctgtgt ctgagcttcc
      721 tgttgatgat tggctttagc ctggtggcgg aaggctttgg cctcgtcata cctaaaggtt
      781 atctgtacgc ggctatcggt ttctcagtga tgatcgagtt cttaaaccag ctggccatct
      841 tcaaccgtcg tcgcttcctc accgctaacc agtcgctgcg aagccgcacg acggaggcgg
      901 ttatgcgcct gctgagcggc aagaaagagg atgccgagct ggatgtcggt accgcctcgc
      961 tgctggtgga ccaggacgat cagcaaatct tcgacccgca ggaacggcgg atgattgagc
     1021 gtgtgctcaa ccttaaccag cgcacggtca gcagtatcat gacttcacgt cacgatatcg
     1081 agcacatcga cctcagcgcg ccggaaagcg aaattcgcgc gctgctggag aaaaaccagc
     1141 atacccgcct ggtagtgacc ggcggcgatg acgaagaaga tctgctcggc gtggtgcacg
     1201 tcatcgatct gttgcagcag tcgctacgcg gtgagccgct ggatctgcgc gtactgatcc
     1261 gccagccgct ggtcttcccg gaggcgctgc cgctgctgtc agcgctggaa caattccgta
     1321 acgcgcgaac ccactttgcc ttcgtggtgg atgagtttgg ttcggtcgag gggattgtga
     1381 cgttaagcga cgtgaccgag accattgccg gtaacctgcc aaacgaggct gaagagattg
     1441 atgcgcgcca cgacatccag aaaaatgccg acggcagctg gacggcaaat ggccacatgc
     1501 cgctggaaga tctggtgcag tacgttccgc tgccgctgga tgaaaagcgc gagtaccaca
     1561 ccattgccgg gctgctcatg gaacacctgc agcgcattcc cacagccggt gaagaggtgc
     1621 agattggccc gtggacgctg aaaaccttac aggtcgagag ccacagggta cagaaagtgc
     1681 agattatccc gccgccgcag gaagagtggg attacgaagt gtaatatttt cccggcggcg
     1741 ccatgccgcc gggcttaggg atgttactgc ttatcgagga gctgcttcac gtctttaccg
     1801 gactgggtat tcttattgcg ttccgcccag tcatttaacc gacgcttcgc ctcgttctgt
     1861 agctgacggc gcagtatctg gtctacctgc aaactgtagt tcagggcttc ccatttgccg
     1921 taaacgcgca gcggaatagc cgtagacttc agaatatcca ccagcttgcc ctgcccctgc
     1981 caaccatcag taacctgaat accgaactgc agatcgcctt cctctttcat cagatcgaga
     2041 gtgcctttgc cggtcatctg catcagcgag gatttccctt tcatgttact gagcgccaga
     2101 atgccgctat cgaggtcaac atcggtcgtc agttcatcca gccgcgtggc gctgtcatag
     2161 ttctcctgcg cctgcacgtc tgtactacgc tcaaccgcct gctgaaccaa ctgctggaag
     2221 ttcagccctt cggtacgggt gtctcttagt tcaagattcg cctgcccctg ccagtcgcgg
     2281 cggaaatggt tggcatcaat caccgaaccc gaaaaatcac cggccagcga cagtttgccc
     2341 gtcatgttga tggggtagtt gaaggccttc aacaggctgc caatctcgat gttgtcgagc
     2401 ttaggctgaa attcggcctg cggagtccca ctgcgcgcat ccagcgtgcc cggcagtgac
     2461 agttgaccac cggctaatga accttccagt tgagagatat gcagcagccc acgctggtta
     2521 tcggcctgca gggtcacatg ggtaaacggc agcccgcgcc agacgatctg gttcgcggtc
     2581 atcgcaagcg aaccatcgaa gcttttcagg ccgctataag gcggtaaatt aacgccgttg
     2641 gctatcaccg gacgcggttt gttcggcgcc gactgtgcct gctgcgcgcc accgccgagg
     2701 aggatatccg agcgcaccag caagttatcg aggttgagct tgtcggactg aaggttcagc
     2761 gaccacaccg gcttatcgcc cagcaccacg ctgcctttac cggtcagcgt actgtcgttg
     2821 gccatcagat tgagcgcgtt aaagctgagc gtcttcgcgt cctcctgcca gctaacgttc
     2881 acgctaccgt taccgttaat gccctgagcc ggaagatcgg cgccctgaag ctgccagctg
     2941 atttgcgaca gctgcgcatc cagactctgc gggtagttgc ccgcctgaac ggcggcggag
     3001 aaacgaaggg ataagtcgcg ctgatcgcgg ttcatgcggc ctgagaaatc gacggtggcc
     3061 tgatgcccgg catcctgctg catctgcaga tgaatatcgc ggaccgtcat ctgctcatca
     3121 ctttcatgct ggaataccag cacgctgtca atgacctgca gcccggcaat atcgaatgac
     3181 cagccgcgat cgccagactc ctgaggcagc gaggttcctt ttggcgccac cggcgctcgc
     3241 tggtcgcgaa tcgcctcggt ttccggcgtc aattgtacca ccgcgccttt gagcatcacc
     3301 tgctcaacct gcagctgatg tgacagcagc ggcagcagcg ccacgtcgag acgcatattg
     3361 tctgcccgca ccagcggcgc gctggcgccg ggcgcggtta aggtcatacg tccggacaaa
     3421 atgcttagcc gcggccagac gtgccagcgc agggaaccgt ccagctccag ctgatatccg
     3481 ctgcgctcag ccacctgctt gatcatatag ctgcggaaat cgttaggatt gaccagcagc
     3541 accaacgatg acaggccggc aaccagtacc accagcagaa tcatcagtgt cgtcaatatt
     3601 cttctcatgg caccctcatc cgctttgcga tgggtcagtc tttatcaata cgactggcaa
     3661 ccgcgccctg ctgatcgcgg tatttggcat cttcacgacg gttgtatgga cgcgccgccg
     3721 ggcccgacag aggttcgaag ctcagtgcac cgatcagcat ccccggacgc agcgccagcg
     3781 gcagcttacc ggagttgtag aattccaaca cgatgcaacc cgaccaaccc ggatcgatac
     3841 ggtgcgcggt gacgtgcacc atcaggccca gacgcgccag cgaggaacga ccgtccaacc
     3901 agccgaccag atccgcgggc agcgtgaccg attccagcgt caccgccagc gccagttcgc
     3961 ccggatgcag atagaaggcg tcgccttccg cgagcacaat ctcttcgctc atcacacgat
     4021 tcagcgccgc gctgacttcc gctttcggcc cgcttaaatc aatgtaggcg gcagtgtggc
     4081 cgctgaaggt gcgaaacttg ttgcctaaac gcacgtcaac ggtggcacca ttgatacgct
     4141 caatcggcgg acgcgggtta atcgacagtc gcccttcgtc cagccaggct tctatatcac
     4201 ggtcacacag acgcatatca ctctcctttt gtacgacggc cctcttccgt cacggttgag
     4261 ggcgtttatt gcacgttaca caattcgtgt gagttattca aaaaactggc taattttcgc
     4321 tttcagaata tcgatagcga tgcggttttt gccgccacgc ggtacgatga tgtcggcgta
     4381 ctgcttggac ggctcgataa actgcaggaa catcggacgc accgttttct ggtattgagc
     4441 catcacggag tccatggagc gaccacgttc gttaacgtca cgcttaatgc ggcgcatcag
     4501 gcagatgtcc agcggcgtat cgacaaagat agagaagttc atctcttcgc gcagacgcgc
     4561 gtcggtcagc agcaggatcc cttcaaggat gatcactttc ttcggttcaa tatgaatcgt
     4621 ttcctgcatg cgggtgtgtt cgacatagct ataaaccggc agttcaattg ggttgccgcg
     4681 cttcagcgtt tgcagatgct ggaacagcag gctgtggtcc atcgcgttcg ggtggtcata
     4741 gttagtttta acccgatctt ccatcgacag atgactttgg tctttgtaat agctatcttc
     4801 aggaatgaca ccgatgtgct catcgcccac ctgttcgcgc agttcgcgat acagcgtact
     4861 agcaataaga cttttacctg aagccgatgc gccggcgatg cctacaatga cgcactgatg
     4921 agacttatca gtcataaatt ttgcgacccg attaacctgg atgttaggaa ggacggcacg
     4981 aatagacaat attattcaag aggaaacgtt ccgaatagtc acgtgtatca gcgccaaacg
     5041 cggcaattat agggatttca gggcaacgat actagtcgaa tacaacgatc ggcgatgaca
     5101 aaaggagaaa cgcccgtacg tcggattcta ctgacgtcat acattaacga atatgatatt
     5161 acaggtgaca caaactgatg attattgtca attagcgtca catatctgat aaattccgct
     5221 aatgttgttg cataatcacg gcttaattca gcatggccgg agcagcgtcg cctccggaaa
     5281 aacagtggta atgaagaaca atatcagcgt taaccctctc cgcccctcgt cacaacagca
     5341 ctcactgcaa atactgtttg gtttatgggc agcgacattt ttgttcaccc tcttttcatt
     5401 atcactcatg cacactcaca atgtgctatc gccggtctgg tttccgacgg ccatcatgac
     5461 cgtcgccttc taccgtgtta agcgttcgct atggccctgc atgagcgttg ctggcgtatt
     5521 ggggattgtc ctcgccaccg agctttacgc tcccgagcag aatggactgt tacacccgtt
     5581 gaccaatctt atggaagcgc tggttggcgc gttactgctg cgccgatggc tgaacctcga
     5641 caacccgcta agaaacttac gccactggac tcaaatggcg atcgccaccg cgctgatccc
     5701 gccattgttg agcggtattg tagtggccag tttgacgccc ggaggcaatg ctttccagac
     5761 cttactggtc tggatgcttt cggatgcctc aggcgccctg gcgttagttc cgctgggaat
     5821 gctcatccgg gcagactatt ttcagcgtta tcgtaaccgc acgctgctga tctcgacgat
     5881 tctgaccgca gccatcaccc tcgcgctcag cgtaatcgcc atgttgtaca tgccctggcc
     5941 gtttaccttc ataatggtac tcatgatgtg gagcgcggta cgtctgccgc gtattgaagc
     6001 cttcctgatc tttttactca tcattatgat ggttctgacc ctctccgcgc agcatccgct
     6061 ggtactggag aacatcgaaa acacccttaa caactaccgg ctgagcaata tgctggggct
     6121 gccattcctg atggtgcaat tgcccacagg ggtaatgagc atcgcgatgt atgccgtata
     6181 cagtgaacgg cgtcgcattg cggaaagtga aacccgcttt cgtaacgcca tggaatactc
     6241 ggccgtcggc atggcgatag tcgatgccta tgggcagtgg ctgcaggtca ataaagcgct
     6301 gagcaagttt cttggatatc cccctgacga actgcatcaa atgaccttcc agcaggtcac
     6361 ctggccggaa gatttagcac aagaccttga acagctggac cgtctgacgc gtggcgaaat
     6421 tgagagctac tcgcttgaaa agcgctaccg tacccgccag ggcgaggcgg tctgggcgct
     6481 gctggccgtc tcgctggtac gcaacgctga tggaacgccg ctctatttca tccaacagat
     6541 tgaagatatt aacgatctga aaaagagccg cgaagccaac aaacggctga tggcggagat
     6601 aaccgaaacc aacgaagcgt tgttccagga aaaagaacgc ctgcacatca ctctcgactc
     6661 cattcgtgaa gcggtgatca gcgccgacag ccagcaaaac attatcttta tgaacccggt
     6721 tgccgagaaa atcagcggct ggaagcaggc tgaggcgcaa gggaaatcat tgcgcgaggt
     6781 gctgtatatt acgcagggag accagggccc gcggctggag aatttgtaca gtgatgaaaa
     6841 cctggatagc gatattgagt tgaacaaagt cctgcatagc cgctacggcg gcagttttga
     6901 tattcaccac agcatgaccc cgctcagtac ctaggaaggc gagcatatcg gttttgtgct
     6961 ggtcattcag gacgtcacgg aatcgcgccg cctgctgcgc cagctcagct acagcgccag
     7021 ccacgacgcg ctcaccgggt tgcccaatcg ttccagcttt gaaagccagc tgaaaggcct
     7081 gctgaaatcg ttgcccggag gacatcagtc gcatgcgctg gtgatgctcg acctcgattt
     7141 cttcaaagcc gtcaacgaca gcgccgggca cgctgccggt gacgcgctac tgcgtgaaat
     7201 tgccgggcta atgcgctcgc tgctgcgacc gggcgatatt ctggcgcgac ttggcggtga
     7261 tgaatttggt tttattctgc gtcactgctc taccgagcag gcgctgagcg ccagcgagcg
     7321 cattgtcgat gccatcaaac attacgaatt tttctgggca ggacggttgc accgtattgg
     7381 cgccagcgcg gggctgacgt tgattcacaa ccataacgcc caactggcgg aggtgctggt
     7441 tcaggccgat gccgcctgct acacctcaaa aaataacggt cgcggtatcg tgacggttta
     7501 taccccggta cattcttccg gcgaccagtc gtcggcgtgc cagatatgca ataacgcgta
     7561 agatcgccag ggctgccaac gttcggcata acggcgaatc tgcgcgggcg tcatacccgg
     7621 gaagcgctgc ttaatcaggt agtcgtccgg caaaaagata tctttcgcct gccaggcgcg
     7681 cagggcaaag tagtttgccg tccaacggcc aatgccggga tattgttgta gcgcctttag
     7741 cgcctgatcc acatcgtctg gctgcgtttg cggcagttct ccgctatgcg cacgctgggc
     7801 aaaatgtatt aacgcttccg cccgtttgag cggcatgccc aatgctttca acgccgccgg
     7861 gtccgccgcc gccagccgct ctggcgttgg gaacaacgag aaagaggcgt cctgtgcgag
     7921 cggctcgcca tacagcgcca ccactttacc ggtcagcttc gcggccatcg tcacgctcac
     7981 cagttgcccg agaatcgccc gcacgccctg ttcaaaggcg tccagcgcgc caggcagtcg
     8041 taaccccggc tttgccgccg ccagcgttcc gagcgcgcgc gatatttgtt gtggatcgca
     8101 gtgcagatcc agcaatttcg ccacccgcat caacgcttcg tcagcgactg gcagcaaccc
     8161 ggcggaaagc gtgacccgca gcacctggcg cgcgggatcc ggcgtcatgg tgataagccc
     8221 atggtgtggc ccgagccccc aactgcgggt atagctttga tcatggaaac attcgatacc
     8281 tgcgaccgca cgcgcctgta aaaaaccgaa catccagcgc cagtcatatg gcggcagcca
     8341 gggcagttca aacatccttt atccttctcg tcagacagca aagatttcgt tacagcatag
     8401 cggttaatca atgggaaaac cttgctttca tattcctgtt gccgccatca ccacattccg
     8461 ctaaagtcgc ccccctttct tcactggcat ggggatgatt gacgtgttta ttggttttga
     8521 ctacgggacg gcgaactgct ctgtcgccgt gatgcaggat ggcgcaccgc gcatgctgat
     8581 gatggaaaac ggcagttccc tactgccgtc catgctgtcg gccccgaccc gcgaagcggt
     8641 cagcgagtgg ctgtaccgcc accatcaggt accggcaacg ggtgatgaaa ctcaggcgct
     8701 gttgcgtcgg gcgatgaact acaaccgcga cgaagatatc gaggtactgg gcaatagcgt
     8761 gcagttcggc ctcgcgtcgc tcaagcagta catcgacgat ccggaagagg tgtactttgt
     8821 taagtcgcct aaatccttcc tcggcgctag cgggctgaag ccgcagcagg tcgccgtctt
     8881 cgaagatctg gtctgttcga tgatgctgca tattcgccag caggcgcagt cacagcttca
     8941 ggaaaccatc gagcaggcgg taattggtcg cccgattaac ttccagggtc tgggtagcga
     9001 tgaagccaac acgcaggcgg taggtattct gcaacgcgcg gcgcagcgtg ccggcttccg
     9061 cgacgtggtt ttccagtacg aaccggtcgc cgccggtctg gatttcgaag ccagcctcag
     9121 cgaagaaaaa cgcgtgctgg tggtcgacat cggtggcggc accaccgact gctcggtgct
     9181 gctgatgggg ccgcagtggc ggacccgtca tgaccgcgaa cagagcctgc tcggccacag
     9241 cggctgtcgc gtgggtggta acgatctgga catcgcgctg gcgtttaaaa gcctgatgcc
     9301 gctgctcggc atgggcggcg aaaccgaaaa aggcatcgcc ctgcccattc tgccgtggtg
     9361 gaacgcggtg gcgatcaacg atgttccggc gcagaccgac ttctacagct cagccaatgg
     9421 ccgcctgctg cgtgatctgg cgcgcgatgc ccgcgatgcg gaaaaggtct ccctgctcta
     9481 caaggtatgg cagcagcgtt taagctatcg tctggtgcgc agcgcggaag agagcaaaat
     9541 cgccttgtcc gaaagcgcac aaatcgatac ccacctgccg tttatcagcg acacgctggc
     9601 caccgccatc agccaggacg ggctggaagc ggcgctgaac cagccgctga ctcgcattct
     9661 tgaacaggtg cagttggcgc tggaaaacag cagtgaaaag ccggacgtta tctatttaac
     9721 cggcggcagc gcgcgctcgc cgctgattaa aaaagcgctg gccgaacagc tgcctggcat
     9781 tcccgttgcc ggtggcgacg attttggctc ggtcaccgcc ggtctggccc gctgggcgca
     9841 ggtggttttc gcctgatcta tcgccattga cttcgctgtt ttttcgacct attgtaaagg
     9901 aatgagggta agggaggatt gctcctcccc ctggtatctt agtaagccgg taagctaatg
     9961 actaagagta ttaccagtat gatgactagc ctcatcataa ccctttcctt ataaaaggcc
    10021 ccttcgtcag gaggggcttt cctgttttag cgccctgcta aacgccgctg acggtagcac
    10081 acgcggcgat tgcgcggttt ttcatcgccg cggattgcga ggagcctcgt aaagctgacg
    10141 cgattcagac gattccttcc gtgtttcata tttcctccat ttttcccgag cgttccgtta
    10201 ctaaactagt atcatttcgt gaaatgattc aggacgagac acctataacg atgaaaggca
    10261 gtcataagat tcgctggcgg gtagcgctgg ccgtgctggt ggtggtcgcg ggggcggctg
    10321 gcatctggca ctttagcagc aattctgaca cctcgggttc caccgcagcc aaaccggtgg
    10381 gagcgccgtc agctggcggc gggcgacacg gtggacgctt tggcggcgcg ctggcgccgg
    10441 tccaggccgc taccgccacc agtgaagcgg taccgcgcta cctcagcggg ctgggcacca
    10501 ttaccgctgc caataccgtt accgtgcgca gccgcgtcga cggccaactg atggccattc
    10561 acttccagga aggccagcag gtcaaagccg gcgatctgct ggcggaaatt gaccccagcc
    10621 agtttaaagt ggcgctggct caggcgcagg ggcagttggc taaagataaa gccacgctcg
    10681 ctaatgcacg tcgtgattta gcccgctatc agcaactggt caaaaccaat cttgtctctc
    10741 gccaggagct ggatacccag caatcgctgg tgagcgaaac cgaaggcaca attaaagccg
    10801 accaggccgc cgtcgccagc gcccagctgc agctcgactg gagccgcatc accgcaccga
    10861 ttgacggtcg cgtgggctta aaacaggtgg atatcggcaa ccagatttcc agcggtgata
    10921 ccaccggtat cgtcgtgctg acccagacgc atcctatcga tttagtcttt accctgccgg
    10981 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg gtggtagaag
    11041 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    11101 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    11161 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    11221 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    11281 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    11341 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    11401 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    11461 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    11521 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    11581 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    11641 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    11701 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    11761 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    11821 aacattaccg ctcgatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    11881 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    11941 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaatatat
    12001 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    12061 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gttcaggcga ttgccgccct
    12121 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    12181 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    12241 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccga ttcgtctggg
    12301 cgatgtcgca accgtagagc aaggtgcaga aaacagctgg cttggcgcat gggcgaacaa
    12361 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    12421 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    12481 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    12541 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    12601 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    12661 ggtgtttctc gatttttcaa tcaataacct gacactgatg gcgttaacta tcgccaccgg
    12721 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    12781 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    12841 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    12901 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    12961 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgctgcgtaa
    13021 acagaaccgc ttctcccgtg cctcggagaa aatgttcgac aggataatcg ccgcctatgg
    13081 acgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    13141 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    13201 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    13261 ccagcgacaa cgccaggtct cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    13321 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaac agtgcacgtt tacaaatcaa
    13381 cctcaaaccg ttggatgaac gtgatgaccg agtgcaaaaa gtcatcgccc gtctgcaaac
    13441 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg atctgactat
    13501 tgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    13561 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    13621 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    13681 cgtcagccgt ctggggatca gcatggcgga tgtcgataac gccctgtaca acgcgtttgg
    13741 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggaaca
    13801 caacaccgaa aatactcctg gcctcgcggc gctggatacc attcgcctga ccagcagcga
    13861 cggtggcgtg gtgccgctaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    13921 catcaaccat ctggatcagt tcccggtaac gaccatctcc tttaacgtgc cggataacta
    13981 ttcgctgggc gatgcggtgc agtcgattat ggacaccgag aagacgctga atctgccggt
    14041 ggatatcacc acgcagttcc agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    14101 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    14161 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg ttggcgcact
    14221 gctggcgttg ctgattgctg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    14281 gctgatcggt atcgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgccga
    14341 gcgcgagcaa ggcatgtcgc cgcgcgaggc aatctatcag gcttgtctgt tacgttttcg
    14401 tccgatcctg atgaccactc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    14461 cggggtcggc gcggaactgc gtcgtccatt aggtattggc atggtcggcg gtctgatagt
    14521 cagccaggtg ctgacactgt tcaccacgcc ggtaatttat ttgctgttcg accgcctggc
    14581 attgtggacc aaaagccgct ttgctcgtca tgaagaggag gcgtaagtga agttttttgc
    14641 cctcttcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    14701 catactgggt ttccgtatgc tgccagtggc cccgctgccg caggtcgatt ttccggtgat
    14761 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcatctt ccgttgccac
    14821 accgctggag cgctcacttg ggcgcattgc cggagtcagt gaaatgacct ccagcagttc
    14881 gctcggcagc acacgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    14941 gcgcgatgtg caggcggcga tcaacgccgc gcaaagtttg ctgcccagcg ggatgcccag
    15001 ccgtccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    15061 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tagctccgac
    15121 gatttcgcaa atcgacggtg ttggtgatgt cgatgtcggc ggcagctcac tgcccgccgt
    15181 acgcgtcggg ctgaatccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    15241 caccgccatc agcaatgcca acgtgcgtaa accgcagggc gcgctggaag atggcactca
    15301 ccgctggcag atccagacca atgatgagct aaaaaccgcc gctgaatatc agccgttgat
    15361 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgaccgactc
    15421 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    15481 ccgcaaactg ccggaagcca atattatcca gacggttgac agcatccggg caaaattacc
    15541 ggagttgcag gaaaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    15601 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    15661 gattctggtg gtgtttttat tcctgcgctc gggtcgcgcc actattattc ccgccgtttc
    15721 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    15781 taacctttcg ttaatggcgc tcaccatcgc tactggtttc gtggtggatg acgccatcgt
    15841 ggtgctggaa aacattgcac gtcatctgga agcgggaatg aaaccgttgc aagccgcact
    15901 gcaaggtact cgcgaagtcg gttttacggt gctgtcgatg agtctgtcac tggtggcggt
    15961 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    16021 gacgctttct gtcgccattg gtatttcgtt gctggtttct ctgacattaa cgccaatgat
    16081 gtgtggctgg atgctgaaag ccagcaagcc gcgcgagcaa aagcgactgc gtggttttgg
    16141 tcgcatgttg gtagccctgc aacaaggcta cggcaagtca ctgaaatggg tgctcaatca
    16201 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct ggctgtatat
    16261 ctcgatcccg aaaaccttct tcccggagca ggacactggc gtgttgatgg gcgggattca
    16321 ggcggatcag agtatttcgt ttcaggcgat gcgcggtaaa ttgcaggatt tcatgaaaat
    16381 tatccgtgac gatccggcag tggataatgt caccggcttt acaggcggtt cgcgagtgaa
    16441 cagcgggatg atgtttatca ccctcaagcc acgcgacgaa cgcagcgaaa cggcgcagca
    16501 aattatcgac cgtctgcgcg taaaactggc gaaagaaccg ggggcgaatc tgttcctgat
    16561 ggcggtacag gatattcgcg ttggtgggcg tcagtcgaac gccagctacc agtacacgtt
    16621 gttatccgac gacctggcgg cactgcgcga atgggagccg aaaatccgca aaaaactggc
    16681 gacgttgccg gaactggcgg acgtgaactc cgatcagcag gataacggcg cggagatgaa
    16741 tctggtttac gaccgcgaca ccatggcacg gctgggaatc gacgtacaag ccgccaacag
    16801 tctgttaaat aacgccttcg gtcagcggca aatctcgacc atttaccagc cgatgaacca
    16861 gtataaagtg gtgatggaag tggatccgcg ctatacccag gacatcagtg cgctggaaaa
    16921 aatgttcgtt atcaataacg aaggcaaagc gatcccgctg tcgtatttcg ctaaatggca
    16981 accggcgaat gccccactat cggtgaatca tcagggatta tcggcggcct cgaccatttc
    17041 gtttaacctg ccgaccggaa aatcgctctc ggacgccagt gcggcgatcg atcgcgcaat
    17101 gacccagctt ggggtgcctt cgacggtgcg cggcagtttt gccggcacgg cgcaggtgtt
    17161 ccaggagacg atgaactcgc aggtgatcct gattatcgcc gccatcgcca cggtgtatat
    17221 cgtgctgggt atcctttacg agagttacgt acatccgctg acgattctct ccaccctgcc
    17281 ctcggcgggc gttggagcgc tgttggcgct ggagctgttc aatgccccgt tcagcctaat
    17341 cgccctgata gggatcatgc tattaatcgg catcgtgaag aaaaacgcca ttatgatggt
    17401 cgattttgcg cttgaagccc aacggcacgg taacctgacg ccgcaggaag ctattttcca
    17461 ggcctgtctg ctgcgttttc gcccgattat gatgaccacc ctggcggcgc tgtttggcgc
    17521 gctgccgctg gtattgtcgg gcggtgacgg ctcggagctg cggcaacccc tggggatcac
    17581 cattgtcggc ggtctggtga tgagccagct cctgacgctg tataccacgc cggtggtgta
    17641 tctctttttc gaccgtctgc ggctgcgttt ttcgcgtaaa cctaaacaaa cggtaaccga
    17701 gtaaatgaca gatcttcccg acagcacccg ctggcaatta tggattgtgg ctttcggctt
    17761 ctttatgcag tcgctggaca ccaccatcgt aaacaccgcc cttccctcaa tggcgcaaag
    17821 cctcggggaa agtccgttgc atatgcacat ggtcattgtc tcttatgtgc tgaccgtggc
    17881 ggtgatgctg cccgccagcg gctggctggc ggacaaagtc ggcgtgcgca atattttctt
    17941 taccgccatc gtgctgttta ctctcggttc actgttttgc gcgctttccg gcacgctgaa
    18001 cgaactgttg ctggcacgcg cgttacagga cgttggcggc gcgatgatgg tgccggtcgg
    18061 caggttgacg gtgatgaaaa tcgtaccgcg cgagcaatat atggcggcga tgacctttgt
    18121 cacgttaccc ggtcaggtcg gtccgctgct cggtccggcg ctcggcggtc tgctggtaga
    18181 atacgcgtcg tggcactgga tctttttgat caacattccg gtggggatta tcggtgcgat
    18241 cgccacactg atgttaatgc cgaactacac catgcagacg cggcgctttg atctctccgg
    18301 atttttattg ctggcggttg gcatggcggt attaaccctg gcgctggacg gcagtaaagg
    18361 tacaggttta tcgccgctgg cgattgctgg cctggtcgca gttggcgtgg tggcactggt
    18421 gctttatctg ctgcacgcca gaaataacaa ccgtgccctg ttcagtctga aactgttccg
    18481 tactcgtacc ttttcgctgg gcctggcggg gagctttgcc ggacgtattg gcagtggcat
    18541 gttgcccttt atgacaccgg ttttcctgca aattggcctc ggtttctcac cgtttcatgc
    18601 cggactgatg atgatcccga tggtgcttgg cagcatggga atgaagcgaa ttgtggtaca
    18661 ggtggtgaat cgctttggtt atcgtcgggt actggtagcg accacgctgg gtctgtcgct
    18721 ggtcaccctg ttgtttatga ctaccgccct gctgggctgg tactacgttt tgccgttcgt
    18781 cctgttttta caagggatgg tcaactcgac gcgtttctcc tccatgaaca ccctgacgct
    18841 gaaagatctc ccggacaatc tggcgagcag cggcaacagc ctgctgtcga tgattatgca
    18901 attgtcgatg agtatcggcg tcactatcgc cgggctgttg ctgggacttt ttggttcaca
    18961 gcatgtcagc gtcgatagcg gcaccacaca aaccgtcttt atgtacacct ggcttagcat
    19021 ggcgttgatc atcgcccttc cggcgttcat ctttgccaga gtgccgaacg atacgcatca
    19081 aaatgtagct atttcgcggc gaaaaaggag cgcgcaatga agttctggcg acccggtatt
    19141 accggcaaac tgtttctggc gattttcgcc acctgcattg tcttgctgat cagtatgcac
    19201 tgggcggtgc gtatcagttt tgagcgtggc tttattgatt acatcaagca tggtaatgaa
    19261 cagcgattac aactgttaag tgatgcgctt ggcgagcagt atgcgcagca tggcaactgg
    19321 cgcttcctgc gcaacaatga tcgctttgtc tttcagatcc tgcgttcatt tgaacacgat
    19381 aattcggaag ataaacccgg cccgggtatg ccaccgcacg gctggcgtac ccagttctgg
    19441 gtggttgatc aaaacaacaa agtgctggtt ggtccgcgag cgccgattcc acctgacggt
    19501 acacggcgac ccattctggt caacggtgcg gaagttggcg cggtgatcgc ctcccccgtt
    19561 gagcggttaa cgcgcaatac tgatatcaat ttcgataaac aacagcggca aaccagctgg
    19621 ttgattgtcg ccctggcaac gttactcgcg gcacttgcca cttttctgct ggcgcgcggt
    19681 ttactggcac cggtaaaacg acttgtcgat ggcacgcaca aactggcggc gggcgatttc
    19741 actacccgcg taacgcccac cagtgaagat gaactgggca aactggcgca agacttcaac
    19801 cagcttgcca gcacactgga gaaaaaccag caaatgcggc gcgattttat ggccgatatt
    19861 tctcacgaac tgcgtacgcc attagcggtg ctgcgcggtg aactggaagc cattcaggat
    19921 ggcgtgcgta aattcacgcc ggagacggtg gcgtctttac aggcggaggt cggtacactg
    19981 accaaactgg ttgacgatct ccatcagttg tcgatgtctg atgaaggcgc tctcgcctat
    20041 caaaaagcac cggtagattt gatcccactg ctggaagtgg cgggcggcgc atttcgcgaa
    20101 cgattcgcca gtcgtggcct gaaactgcaa ttttccctgc cagacagtat taccgtattt
    20161 ggcgatcgcg accgtttaat gcagttattc aataatttac ttgaaaacag cctgcgctac
    20221 actgacagcg gcggcagcct gaaaatctct gccgagcagc acgacaaaac ggtgcgcctg
    20281 acctttgccg acagtgcgcc aggtgtcagt gacgagcagc tacaaaaatt atttgaacgt
    20341 ttttatcgca ccgaaggttc ccgcaaccgc gccagcggtg gttccgggct ggggctggcg
    20401 atttgcctga acattgttga agcacataat ggccgcatta ttgctgccca ttcgcctttt
    20461 ggcggggtaa gcattacagt agagttaccg ctggaacggg atttacagag agaagtatga
    20521 ccgagttacc aatcgacgaa aacacaccgc gtattttgat cgtggaagat gaaccgaagc
    20581 tggggcagtt gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg cttatcagcc
    20641 acggcgatca ggtactgccg tatgtgcgcc agacaccacc ggatctgatc ctgttggatc
    20701 tgatgctccc tggcaccgat ggcttgacgc tgtgccggga aattcgtcgt ttttctgaca
    20761 ttcctatcgt gatggtgacg gcaaaaatcg aagagatcga ccgcctgctc ggactggaga
    20821 ttggcgcaga tgactacatc tgcaagccgt acagcccacg ggaagtggta gcgcgcgtca
    20881 aaaccatttt gcgccgttgc aaaccgcagc gcgaattgca gcaacaggat gccgaaagcc
    20941 cgttgattat cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg cttgacctga
    21001 cacctgcgga atttcgtctg ttgaaaacgc tctctcatga accaggaaaa gtgttctccc
    21061 gcgagcaatt gctcaatcat ctttatgacg actaccgcgt agtaaccgac cgcaccattg
    21121 acagccacat taaaaacctg cgccgcaagc tggaatctct cgacgccgaa cagtcattta
    21181 tccgcgccgt ttacggcgtt ggttaccgct gggaagccga cgcctgccgc atcgtttagt
    21241 tttagcgaca tgactttgtt agccggagat ggcgttcaga ccagatccgg caacattatc
    21301 ccacgcatgg tcagcaaact gacattatct ctccgccctg gcaggcatca gaaaatcgct
    21361 catactttaa tcggtaaaca gcacctttag atgctgtttt cgatacgcaa tttcaatcaa
    21421 ggagtcatta tggctggttg gtttgaactc agtaagagca gtgataatca gttccggttt
    21481 gtgctaaaag cgggcaatgg tgagactatc ctcaccagcg agctgtatac ctcaaaagcc
    21541 tctgcggaaa agggcatcgc gtcggtgcgt agcaacagcc cgcaagaaga acgctatgag
    21601 aaaaaaacgg caagtaacgg caaattctat ttcaatctga aagccgctaa tcatcaaatt
    21661 atcggctcca gccagatgta cgccaccgcg caatctcgtg aaaccggaat tgcctccgtt
    21721 aaagccaatg gcacaagcca gacggtgaaa gacaatacgt aattacgatg ccgagcgtgg
    21781 gttgttggcg tccggcttta tatccaaata acgcttagtt attaataatt aagagtaacc
    21841 gccgccatta aaatagtggg cactgacgag cactcgttgt gctttctttg tttaccatcc
    21901 catacttatt actcctgaac atgttcatgt gccaaaatat cttctaatac tttcgtgacg
    21961 ctatccggtt tatgacatac actgatgtaa gatttttact tacaggcggg cgtaaaatgg
    22021 ccagaacgat gacggttgat ctcggcgatg agttgcgtga atttatcgag tccttaattg
    22081 aatccggcga ctatcgcacc caaagcgaag tgatccgtga gtcactgcga ttacttcgag
    22141 aaaagcaagc agagtctcgc ctgcaagcgt tacgtgacat gctcgcagaa gggttgagta
    22201 gtggtgaagc tcagccgtgg gaaaaggacg cgttcttacg gaaggtgaaa gcagggataa
    22261 ggaaataatg tgcgtattat taaactcatg ccaaaagcca atgaggattt agaaggcatc
    22321 tggtattaca gctatcacca tttcggcgaa ccacaagccg acagatatgt ggaacatctt
    22381 tcagacgttc tccagattct gagtaacaat aatataggta cgcccagacc tgaattaggc
    22441 gaaggtattt ttgttcttcc ttttgaacgc cacgtcattt atttcctgca atcaccaggg
    22501 gaaattatcg tcattcgtat ccttaaccaa aaccaggacg ccaccagaca tttacactgg
    22561 agctaaaccc gccaattgcg ctacaatgcc cgcccttaaa gtgggggcac tcccctaacc
    22621 gcttcatcag gtgaagcgga tctgacctgt catcagaacg agagaattat gtttaaaccg
    22681 gaactccttt ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc ttatggcgca
    22741 gatgctgttt atgcgggcca gccgcgttat tccctgcgtg tgcgcaacaa cgaattcaac
    22801 cacgaaaatc ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa gttttatgtc
    22861 gtggtcaaca ttgcaccgca caacgccaag ctgaaaacct ttatccgtga cctgaaaccg
    22921 gtggtggaaa tggggccgga tgcgctgatt atgtccgatc cagggctgat tatgctggtg
    22981 cgtgagcact tccctgaaat gccgatccac ctttcggtgc aggctaacgc cgtgaactgg
    23041 gcgacggtga aattctggca gcaaatgggc ctgacccgcg tgatcctctc tcgcgagctg
    23101 tcgctggaag agattgaaga gatccgcaat caggtgccgg atatggagat cgagatcttc
    23161 gttcacggcg cgctgtgcat ggcctactcc ggtcgctgcc tgctctctgg ctatatcaac
    23221 aagcgcgacc cgaaccaggg cacctgcacc aacgcctgcc gctgggagta caacgtccag
    23281 gaagggaaag aagatgatgt tggcaacatc gtacacaagt acgagccgat tccggtgcaa
    23341 aatgttgagc cgacgctggg tatcggcgca ccaaccgaca aagtgtttat gatcgaagag
    23401 gcccagcgtc cgggcgagta tatgaccgcg tttgaagatg agcacggcac ttacatcatg
    23461 aactcgaaag atctgcgcgc catcgcccat gtagaacgcc tgaccaaaat gggcgtgcat
    23521 tcgctgaaaa tcgaaggtcg taccaaatct ttctactatt gtgcacgcac cgcacaggtt
    23581 taccgcaaag ctatcgatga cgccgctgcg ggaaaaccgt tcgataccag cctgctggaa
    23641 actctggaag gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg tcatactcac
    23701 gacgattatc agaactacga atacggttat tcagtttctg accgccagca gtttgttggt
    23761 gagtttaccg gtgagcgcaa gggggacctc gcggcggtag cggtgaaaaa taaattctcc
    23821 gttggcgaca gccttgagct gatgacgccg caaggcaaca ttaattttac ccttgagcac
    23881 atggaaaacg ccaaaggcga agctatgccg atagcaccag gcgatggtta tactgtgtgg
    23941 ctcccggtcc cgcaggatct tgagctcaat tacgcgctgc tgatgcgtaa tttctccggg
    24001 gaaaccacgc gtaatcccca cggtaagtga ttaatttcga ttatttttcc cggatggaaa
    24061 attcttagaa accgatcaca tacagctgca tttattaagg ttatcatccg tttcgctgaa
    24121 aaacataacc cataaaatgc tagctgtacc aggaaccacc tccttagcct gtgtaatctc
    24181 ccttacacgg gcttattttt tatgcataag ccctatccct ggtcaccgtc ttccattgac
    24241 cacatcgata gaatctccct tcatagcacg atgcctttca cgtaacggca tcgtgctcgc
    24301 acaggttccg gctaagcaca accagaacgc gcatgtttga tgcttaccaa aaaatattct
    24361 cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc ttctgtcttc
    24421 aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc atcatttttt
    24481 actatatttt ctgcaccaga tattctaccc ctggctaaag aagcttcggc ttcggtgtag
    24541 tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa tttaccgata
    24601 cttgtctcat cattatgcac agcacaacca gatataagaa aaatactagt taatgatata
    24661 gcagctatct ttttcatctc accttccccc atta
//
LOCUS       DAJSKJ010000047        24484 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000047 DAJSKJ010000000
VERSION     DAJSKJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.047"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            5..1996
                     /gene="pgaB"
                     /locus_tag="ON345_001613"
     CDS             5..1996
                     /gene="pgaB"
                     /locus_tag="ON345_001613"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCR3071693.1"
                     /translation="MMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPWPHNGFVAIS
                     WHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGKPLPEKAVVL
                     TFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVDREYFATWQQ
                     VREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIR
                     LDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLD
                     SIPRVLIANNPSLKEFAHQIITVQEKSPQRIMHIDLDYVYDENLQQMDRNIDVLIQRV
                     KDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAWM
                     PVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAF
                     DGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDF
                     TLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDWTAIMAMPYM
                     EGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISSQQLAHWMSL
                     LQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            1989..3314
                     /gene="pgaC"
                     /locus_tag="ON345_001614"
     CDS             1989..3314
                     /gene="pgaC"
                     /locus_tag="ON345_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCR3071694.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGTAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIHAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            3316..3729
                     /gene="pgaD"
                     /locus_tag="ON345_001615"
     CDS             3316..3729
                     /gene="pgaD"
                     /locus_tag="ON345_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCR3071695.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(3779..4567)
                     /gene="phoH"
                     /locus_tag="ON345_001616"
     CDS             complement(3779..4567)
                     /gene="phoH"
                     /locus_tag="ON345_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR3071696.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRPALCQRTLHAYS"
     gene            complement(5187..6458)
                     /gene="efeB"
                     /locus_tag="ON345_001617"
     CDS             complement(5187..6458)
                     /gene="efeB"
                     /locus_tag="ON345_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR3071697.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(6464..7591)
                     /gene="efeO"
                     /locus_tag="ON345_001618"
     CDS             complement(6464..7591)
                     /gene="efeO"
                     /locus_tag="ON345_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR3071698.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(7649..8479)
                     /locus_tag="ON345_001619"
     CDS             complement(7649..8479)
                     /locus_tag="ON345_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR3071699.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(9145..10653)
                     /gene="putP"
                     /locus_tag="ON345_001620"
     CDS             complement(9145..10653)
                     /gene="putP"
                     /locus_tag="ON345_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR3071700.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(10812..11021)
                     /locus_tag="ON345_001621"
     CDS             complement(10812..11021)
                     /locus_tag="ON345_001621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071701.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            11076..15038
                     /gene="putA"
                     /locus_tag="ON345_001622"
     CDS             11076..15038
                     /gene="putA"
                     /locus_tag="ON345_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR3071702.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPTEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALASGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(15078..15716)
                     /gene="rutR"
                     /locus_tag="ON345_001623"
     CDS             complement(15078..15716)
                     /gene="rutR"
                     /locus_tag="ON345_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR3071703.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            16004..17095
                     /gene="rutA"
                     /locus_tag="ON345_001624"
     CDS             16004..17095
                     /gene="rutA"
                     /locus_tag="ON345_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR3071704.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDYFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            17095..17787
                     /gene="rutB"
                     /locus_tag="ON345_001625"
     CDS             17095..17787
                     /gene="rutB"
                     /locus_tag="ON345_001625"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR3071705.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            17799..18185
                     /gene="rutC"
                     /locus_tag="ON345_001626"
     CDS             17799..18185
                     /gene="rutC"
                     /locus_tag="ON345_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR3071706.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            18193..18993
                     /gene="rutD"
                     /locus_tag="ON345_001627"
     CDS             18193..18993
                     /gene="rutD"
                     /locus_tag="ON345_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR3071707.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            19003..19593
                     /locus_tag="ON345_001628"
     CDS             19003..19593
                     /locus_tag="ON345_001628"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR3071708.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            19604..20098
                     /gene="rutF"
                     /locus_tag="ON345_001629"
     CDS             19604..20098
                     /gene="rutF"
                     /locus_tag="ON345_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR3071709.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            20119..21447
                     /gene="rutG"
                     /locus_tag="ON345_001630"
     CDS             20119..21447
                     /gene="rutG"
                     /locus_tag="ON345_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR3071710.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(21531..21704)
                     /locus_tag="ON345_001631"
     CDS             complement(21531..21704)
                     /locus_tag="ON345_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR3071711.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            22077..22673
                     /gene="wrbA"
                     /locus_tag="ON345_001632"
     CDS             22077..22673
                     /gene="wrbA"
                     /locus_tag="ON345_001632"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR3071712.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            22694..22921
                     /locus_tag="ON345_001633"
     CDS             22694..22921
                     /locus_tag="ON345_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR3071713.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(22959..24200)
                     /gene="agp"
                     /locus_tag="ON345_001634"
     CDS             complement(22959..24200)
                     /gene="agp"
                     /locus_tag="ON345_001634"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR3071714.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVFMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         5839 a         6454 c         6294 g         5897 t
ORIGIN      
        1 tctcctgatg ctggtgagta taattatgct caccgcgtgc attagccagt caagaacatc
       61 atttataccg ccacaggatc gcgaatcttt actcgccgag caaccgtggc cgcataatgg
      121 ttttgtagcg atttcatggc ataacgttga agacgaagct gccgaccagc gttttatgtc
      181 agtgcggaca tcagcactgc gtgaacaatt tgcctggctg cgcgagaacg gttatcaacc
      241 ggtcagtatt gctcaaattc gtgaagcaca tcgaggagga aaaccgctac cggaaaaagc
      301 tgtagtgctg acttttgatg acggctacca gagtttttat acccgcgtct tcccaattct
      361 tcaggccttc cagtggcctg ctgtatgggc ccccgtcggc agttgggtcg atacgccagc
      421 ggataaacaa gtaaaatttg gcgatgagtt ggtcgatcga gaatattttg ccacgtggca
      481 acaagtgcga gaagttgcgc gttcccggct cgttgagctc gcttctcata catggaattc
      541 tcactacggt attcaggcta atgccaccgg cagcttattg cctgtatatg taaatcgtgc
      601 atattttact gaccacgcac ggtatgaaac cgcagcagaa taccgggaaa gaattcgtct
      661 ggatgctgta aaaatgacgg aatacctgcg tacaaaggtt gaggtaaatc cacacgtttt
      721 tgtttggcct tatggcgaag cgaatggcat agcgatagag gaattaaaaa aactcggtta
      781 tgacatgttc ttcacccttg aatcaggttt ggcaaatgcg tcgcaattgg attccattcc
      841 gcgggtatta atcgccaata atccctcatt aaaagagttt gcccatcaaa ttattaccgt
      901 acaggaaaaa tcaccacaac ggataatgca tatcgatctt gattacgttt atgacgaaaa
      961 cctccagcaa atggatcgca atattgatgt gctaattcag cgggtgaaag atatgcaaat
     1021 atcaaccgtg tatttgcagg catttgctga tcccgatggt gatgggctgg tcaaagaggt
     1081 ctggtttcca aatcgtttgc taccaatgaa agcagatatt tttagtcggg ttgcctggca
     1141 attacgtacc cgctcaggtg taaacatcta tgcgtggatg ccggtattaa gctgggattt
     1201 agatcccaca ttaacgcgag taaaatactt accaacaggg gagaaaaaag cacaaattca
     1261 tcctgaacaa tatcaccgtc tctctccttt cgatgacaga gtcagagcac aagttggcat
     1321 gttatatgaa gatcttgccg gacatgctgc ttttgatggc atattgttcc acgatgatgc
     1381 tttgctttca gattatgaag atgccagtgc accggctatc acggcttatc agcaagcagg
     1441 ctttagcggg agtctgagcg aaattcgaca aaacccggag caatttaaac agtgggcccg
     1501 ctttaaaagt cgtgcgttaa ctgacttcac tttagaactt agtgcgcgcg taaaagccat
     1561 tcgcggtcca catattaaaa ctgcacgaaa tatttttgca cttccggtaa tacaacctga
     1621 aagtgaagcc tggtttgcac agaattatgc tgatttccta aaaagctatg actggaccgc
     1681 tattatggct atgccttata tggaaggtgt cgcagaaaaa tcggctgacc aatggttaat
     1741 acaattgacc aatcaaatta aaaacatccc tcaggctaaa gacaaatcta ttttagaatt
     1801 acaggcacaa aactggcaga aaaatggtca gcatcaggct atttcttcgc aacaactcgc
     1861 tcactggatg agcctattac aactgaatgg agtgaaaaac tatggttatt atcccgacaa
     1921 ttttctgcat aaccaacctg aaatagacct tattcgtcct gagttttcaa cagcctggta
     1981 tccgaaaaat gattaatcgc atcgtatcgt tttttatatt atgtctggtg ttatgcatac
     2041 ccctatgcgt agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc
     2101 cgttttttat gtccattatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac
     2161 gccactggcc gtggggagaa aacgcaccag ctccccagtt gaaagataat ccgtctatct
     2221 ccattatcat tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt
     2281 tagcacagcg ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa
     2341 cccgtgccat cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg
     2401 cgcaaaacca ggggaaagcc attgcgctta aaaccggaac tgccgcggcg aaaagtgaat
     2461 atctggtgtg cattgatggc gatgcgttat tagaccgcga tgcggcggca tatattgtgg
     2521 aaccgatgtt gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac
     2581 gttctaccct ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca
     2641 agcgaaccca gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc
     2701 gtcgcagcgc cctagcagaa gtgggttact ggagtgacga tatgatcacc gaagatattg
     2761 atattagctg gaagctgcag ttgaatcagt ggacgatttt ttacgagcca cgggcgctgt
     2821 gctggatatt aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc
     2881 agggcggtgc agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc
     2941 gaatgtggcc gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg
     3001 tcggtttcat tattcacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac
     3061 atatcgctgc gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta
     3121 ttgtcagcct gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga
     3181 ttatttggtt cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta
     3241 cacgagtcat gttgatgcct aaaaagcaac gcgcccgttg ggtaagtccc gatcgcggga
     3301 ttctgagagg ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta
     3361 ctggttgatt atgttgccac aaccatcttg tggacattat ttgcgttgtt catattctta
     3421 ttcgccatgg atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag
     3481 ttctattttt tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat
     3541 aagctgcgtt ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat
     3601 gcagagagct tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg
     3661 agcgtacact tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc
     3721 cgggcataaa cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt
     3781 aactgtatgc atgcagcgta cgttggcaaa gtgccggacg tacgcagtcc tctttaccga
     3841 accggacgat cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg
     3901 agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt
     3961 tctccccgag gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt
     4021 caagaatgac gactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcaattt
     4081 ccaccttccc tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga
     4141 ccagcacgtc gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tctccaggta
     4201 agaagccaag atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat
     4261 ccttatgtat cagggcttct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg
     4321 cttcgcccgt ggcgaatatc agctgcttac tctcaatagc cttcagatag tgcaattgcg
     4381 cttcatttcg cgcgaggatg ggcgaagtat cgcgactgtc gcgggccata ccaatggctt
     4441 ctacgccgcc catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc
     4501 gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc
     4561 ttcccatgga gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat
     4621 tatgcgcacg aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa
     4681 atgccgaaaa acggctacgc gcaccgttta cggcgtcggt aacacatgaa aagaaaggat
     4741 gaggttgaaa atgcaaagtg acgagatgac taccggagga gaaaactccg cgagtggtgg
     4801 cgcgttgatt atctaaaaca tgtccagtac aggacgttac catccgcgat ctccatagtg
     4861 actgactatc actgccggga acttccgctg ctacttaata agtacaacag atctcgcatt
     4921 tattgcaaca atatatttac ttatatttaa ctataaaaca ccatttcagt gacattagtt
     4981 tctactggaa agatgacaga gtgatgacag atatgaaaaa agctgtgtgc tttcagcagg
     5041 atttgcatca ggcatcagtg cacgatcgcc gtatgcggcg tgaacgcctt atccgcctac
     5101 cgatcccgca ccgattgtag gcataagaag acgcgcaagc atcgcatcag gcatctgcgc
     5161 aagaacgcct tatccgtcta aaaacattaa acccgcaata acgcgcttcc gagataatcg
     5221 ttcgcgtcct tcactcccgg cagcgcaaaa aaataaccgc cgccgatagg tttaacgtat
     5281 tcctccagcg cttcgccatt gagccttttt tgtactgtca ggaagccttt ttccagatcg
     5341 tgttggtaac agacaaacag tagccccata tccagttgcc cggagttggt gacgcccagt
     5401 gaataactgt agccacgacg cagcatcagg ctggactcac tctccgccgt gcggggattc
     5461 gccagccgga tatggctgtc cagcgcaatc accttccctt ccgggtcgct ggcgtaatca
     5521 ggcacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acggccaaaa
     5581 atcgtctgct gttctttcag cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag
     5641 cgtactgcct gatagctgcc accgattgtc cacgcaggct cctgctgatc tgccgttacc
     5701 cacaccactt tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtctttg
     5761 aaacccagca aattaatcgg cgtctctttg cctttactac gcgccgcgtg atcggaaata
     5821 aacccttccc gcttccagcg cacactgagc aaatccggcg tgtgtttgat gatatcgcgc
     5881 agcgcatgga taaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac atcaccatga
     5941 cataacgccg catccagcga gtcgttgggg aaacgcgtca tcttctgcag cttttttggc
     6001 atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga taacgtgatg
     6061 gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc
     6121 ggatttggcg tttctggtgc tgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac
     6181 aagcgaaaca accgctcaag atcggcttta tcgctggcaa gcacatcaaa cgccaccagc
     6241 atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt
     6301 ttctcattgc gagcatccgg tgaaagcgtg cccggtgcac tttgcgtttt ttgtgcatga
     6361 gcgaccggac aacttcccgc cagcgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     6421 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     6481 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     6541 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     6601 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     6661 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     6721 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     6781 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     6841 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     6901 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     6961 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     7021 agaacagttc agcaatcggt tcaatgcgct catagtgctg gcgcgtcggc gcatacagtg
     7081 ctttcgcttt ttcgatatcg ccagctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     7141 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     7201 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     7261 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     7321 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     7381 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     7441 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     7501 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     7561 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     7621 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     7681 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     7741 cgctgacgct cggcgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     7801 agtgagttga gagcaccgca ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     7861 cggcttcatg aaatgcgcga atggcaccag ctgccagccc tgcggcaacg aagagaataa
     7921 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     7981 ggaagcctag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     8041 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     8101 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     8161 tatcgactgc ctgctccagt tgtactttga cgttgcgcga cactttgcgc atccagaaaa
     8221 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     8281 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat ccccaagccc aggcacaacg
     8341 cagcggcaag caacacgcca atccacatca caccaatcca tcggcctcgc tgggtacgct
     8401 taagatagct ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg cgcaacataa
     8461 tgagaaacgg aacaaacatg ccacccaccc ttaaacgttt ttctgagtca atctatgcaa
     8521 agaaaagtaa atcgcagtga tagcgattat cattactgta aagaaaaata caagcggaat
     8581 gtttataaaa tgatgttgaa atgtaaacaa agagaggtaa tgccagggga tataaacgat
     8641 tattccaggt acaaagatgg gattgtttac aacgagccat gtgttcttgc cggatgccgc
     8701 gtaaacgccg tatccagccg acaaaaccgt cctgactcaa tacatcaaaa cttccatgta
     8761 aaactgataa gcgggtgtat caagcaattt cgtccatcct cgattatcaa atctaacttt
     8821 ttgcccggaa cataagaaca gcgcgggtgt tggtggcgtc aaaatcgctg aagcgttccc
     8881 cgaaggccgg ggcagcccac atggatgtgg gctgagggcg cgtcttacag gacgttacct
     8941 cgcgcccgcc ccggtagccg aaggggataa gctgaaggta ccgcgcagcg gcgattttgt
     9001 tcgccagagc ccgggggtgc agggggccgc ggcgattggc cgccccctgc gcgctccctg
     9061 caccattcaa tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa
     9121 caaaaccgcc gcaggctaag tcccttagct ttcctgcaac cgtgacggcg gagccgaatg
     9181 atagtgcgca tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag
     9241 caaactaaac actacaatcc cgatactgcc gaagatgaag cccggaataa tttcgtagag
     9301 tcccagccag ccgaactgct tccagacgat aaccgtcagc gcaccgatga tcatccccgc
     9361 cagcgcaccg ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa
     9421 cgccgcgcca aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt
     9481 tgccgccagc gcaatcgcca ccagcgccac caccagcacc atcacacgcc ctacccacac
     9541 cagctctttc tggctggcat gtttacgcag aaacgctttg tataaatctt cggtaatcgc
     9601 actggagcac accagcagct ggcagcttaa cgttgacatt accgccgcca gaattgccga
     9661 cagcagaatc ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg
     9721 ctcggcgttc tggtttaccg cgcctgctac cgccgggtgc tcattaaagt aagcaatccc
     9781 aaagaagccg acagccaccg cccctgccag gcagaggatc atccaggtca tactgatacg
     9841 acgcgcatgg acaatgctgt ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg
     9901 ctgcccgaag taacccagcc cccaacccat cagtgagata atggcaacaa agttcagacc
     9961 tttgagcata tcaacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa
    10021 gccaccgaca ctgataatga cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct
    10081 ggcctgtaca gtatcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat
    10141 cgtcgccgca gctcccgccc acagagccgt ttcgtagctc atgccaaagg tactttcaaa
    10201 cagacgcgcg cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac
    10261 cagcgcggag ataatgcgca aaatgcggct tttatcttca aagcgcccgg tgaaataatc
    10321 cggcagggtt aaggcgttat tgttgtattc ggtatgcaca cgcaaccgcc cggccaccag
    10381 cttccagttg atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc
    10441 ggaaagaaaa accgcgcccg gcaaccccat taacagccag ccgctcatat ccgacgcacc
    10501 cgccgataat gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa
    10561 gtttttcgtt gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca
    10621 aaatgtcacc aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg
    10681 caatgtcgaa acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta
    10741 ccagggcgac cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt
    10801 tacattaaat tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt
    10861 tttgcggttg cacctttcaa aaaggttaac tgccgcagag aaaaagtctg agttattttt
    10921 ttaatccctg tcatatcgat ttcttttatt aacatttcat tcatttttaa gcttgctacg
    10981 caggtcacat ttaacatggt tgcacaaagt tgcaacatca tggatatttc acgataacgt
    11041 taagttgcac ctttcagaac aacaggagta atggcatggg aaccaccacc atgggggtta
    11101 agctggacga cgcgacgcgt gagcgtatta agtctgccgc gacacgtatc gatcgcacac
    11161 cacactggtt aattaagcag gcgatttttt cttatctcga acaactggaa aacagcgata
    11221 ctctgccaga gctacctgcg ctgctttctg gcgctgccaa tgagagcgat gaagcaccga
    11281 ctccggcaga ggaaccacac cagccattcc tcgactttgc cgagcaaatt ttgccccagt
    11341 cggtttcccg cgccgcgatc accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta
    11401 tgctgctgga acaagcccgc ctgccgcagc cagttgctga acaggcgcac aaactggcgt
    11461 atcaactggc cgataaactg cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc
    11521 aggggttatt gcaggagttt tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg
    11581 cagaagcgtt gttgcgtatt cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa
    11641 tcagcaacgg taactggcag tcacacattg gtcgtagccc gtcactgttt gttaatgccg
    11701 ccacctgggg gctgctgttt actggcaaac tggtttccac ccataacgaa gccatcctct
    11761 cccgctcgct gaaccgcatt atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg
    11821 atatggcgat gcgcctgatg ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt
    11881 tagccaatgc ccgcaagctg gaagagaaag gtttccgtta ctcttacgat atgctgggcg
    11941 aagctgcact gactgccgca gacgcacagg cgtatatggt ttcctatcag caggcgattc
    12001 acgccatcgg taaagcgtct aacggtcgtg gcatctatga agggccgggc atttcaatca
    12061 aactgtcggc gctgcatccg cgttatagcc gcgcccagta tgaccgggta atggaagagc
    12121 tttacccgcg tctgaaatca ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca
    12181 tcgatgccga agaggccgat cgtctggaga tctccctcga tctgctggaa aaactctgtt
    12241 tcgagccgga actggcaggc tggaacggca tcggttttgt tattcaggct tatcaaaaac
    12301 gctgcccgtt ggtgattgat tacctgattg atctcgccac ccgtagccgt cgccgtctga
    12361 tgattcgtct ggtgaaaggc gcgtactggg atagtgaaat taagcgtgcg cagatggacg
    12421 gccttgaagg ttatccggtt tatacccgca aggtgtatac cgacgtttct tatctcgcct
    12481 gtgcgaaaaa actgctggcg gtgccgaatc taatctaccc gcagttcgcg acgcacaacg
    12541 cccatacgct ggcagcgatt tatcaactgg caggtcagaa ctactatccg ggtcagtacg
    12601 agttccagtg cctgcatggt atgggcgagc cactgtacga gcaggtcacc gggaaagttg
    12661 ccgacggtaa acttaaccga ccgtgtcgta tttatgcccc ggttggcaca catgaaacgc
    12721 tgctggcgta tctggtgcgt cgcctgctgg aaaacggtgc taacacctcg tttgttaacc
    12781 gtattgccga cacctctttg ccactggatg aactggtcgc cgatccggtc actgctgtag
    12841 aaaaactggc gcaacaggaa gggcaaactg gattaccgca tccgaaaatt cccctgccgc
    12901 gcgatcttta cggtcacggg cgcgacaact cggcagggct ggatctcgct aacgaacacc
    12961 gtctggcctc gctctcctct gccctgctca atagtgcact gcaaaaatgg caggccttgc
    13021 caatgctgga acaaccggta gcggcaggtg agatgtcgcc cgttattaac cctacggaac
    13081 cgaaagatat tgtgggctat gtgcgtgaag ccacgccgcg tgaagtagaa caggcgctgg
    13141 aaagtgcggt taataacgcg ccaatctggt ttgccactcc tccggctgaa cgcgcggcga
    13201 ttttgcatcg cgctgccgtg ctaatggaaa gccagatgca gcaactgatt ggtattctgg
    13261 tgcgtgaagc cggaaaaacc ttcagtaacg ccattgccga agtgcgcgaa gcggtcgatt
    13321 ttctccacta ctacgccgga caggtgcggg atgatttcgc taacgaaacc caccgtccat
    13381 tagggcctgt ggtgtgtatc agcccgtgga atttcccgct ggcgattttc accgggcaga
    13441 tcgctgccgc actggcgtca ggtaacagcg tactggcaaa accggcagaa caaacgccgc
    13501 tgattgccgc gcaagggatc gccattttgc tggaagcagg tgtaccgcca ggcgtggtgc
    13561 aattgctgcc aggtcggggt gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc
    13621 gcggggtgat gtttaccggt tcaaccgaag tcgctacgtt actgcaacgt aatatcgcca
    13681 gccgcctgga cgctcagggt cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg
    13741 cgatgattgt cgattcttca gcactgaccg aacaggtcgt cgtggatgta ctggcctcgg
    13801 cgttcgacag tgcgggtcag cgttgttcgg cgctgcgcgt gctgtgcctg caagatgaga
    13861 ttgccgacca cacgttgaaa atgctgcgcg gcgcaatggc cgaatgccgg atgggtaatc
    13921 cgggtcgcct gaccaccgat atcggtccgg tgattgatag cgaagcgaaa gccaatattg
    13981 agcgccatat tcagaccatg cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa
    14041 acagcgaaga tgcccgtgaa tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac
    14101 tggatgactt tgccgaattg cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt
    14161 acaaccgtaa ccagctacca gagctgatcg agcagattaa cgcttccggt tatggtctga
    14221 cgcttggcgt tcatacgcgt attgatgaaa ccatcgccca ggtcactggc tcggcgcatg
    14281 tcggtaacct gtacgttaac cgtaatatgg tgggcgcagt agtcggggtg caaccgttcg
    14341 gcggcgaagg gttatccggc accgggccaa aagcaggtgg cccgctctat ctctaccgtc
    14401 tgctggcgaa tcgcccggaa agtgcgctgg cagtaacgct cgcgcgtcag gatgcagagt
    14461 atccggtcga tgcgcagttg aaagccgcat tgactcagcc gctaaatgca ctgcgggaat
    14521 gggcagcaaa tcgtccagaa ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg
    14581 caggaacaca acgattgctg ccagggccga cgggtgaacg caacacctgg acgctgctgc
    14641 cgcgtgagcg cgtgttgtgt attgccgatg atgagcagga tgcgctgact cagctcgccg
    14701 ccgtgctggc ggtgggcagc caggtactgt ggccggatga cgcgctgcat cgtcagctag
    14761 taaaggcatt gccatcggca gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa
    14821 ccgctcaacc ctttgatgcg gtgatcttcc acggtgattc ggatcagctt cgcgcattgt
    14881 gtgaagccgt tgctgcgcgg gatggcgcaa ttgtttcggt gcagggtttt gcccgtggcg
    14941 aaagcaatat ccttctggaa cggctgtata tcgaacgttc gctgagtgtg aataccgctg
    15001 ccgctggcgg taacgccagt ttaatgacga tcggttaaac aggtagccgg aggatgttac
    15061 aacctcctcc ggcatcttta acgtggtcga atcccctcaa taataatccg ctgcacgttt
    15121 tcaaccgttt gattgaaaaa tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc
    15181 tgaggggcga aatcggcgta atgttgagtg gaagcccaaa tcataaaaat caaatgctgc
    15241 ggatcaatcg gcgcgagttt gccgcttttg acccaaccgg caatcagcgc cgatttctca
    15301 tcaattaatg ccttcaaatc gcccgtcagt tcatccatta acagcggcgc gcctgccagc
    15361 atctccatac agaacaggcg cgaagcctgc ggataatcgc gtgagacttc cagcttcaga
    15421 cggatgtact ctttgatcgc cgccagcggg gcgaaatctt cacgaaacgc ttttaacggt
    15481 gccagccaga tatcgagaat ctgccgcagc acggcaatat acagcgcctc ttttgacggg
    15541 aagtaataca gcagattggt ttttgaaaca cccgccaact ctgcgatctg ctccagcctt
    15601 gtgccgtgaa aaccgaattg tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc
    15661 ttcgcgctta ctgcgcgcga acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc
    15721 tctcccctct tgtagattgg caacaggata gcaaaacccc cgcccggcct cgaccttctg
    15781 cactctcatc gcgcacagat gcatgtttta tgtgcaactg ttttgaccgt ttagtccact
    15841 ttttaccaga tattttagac aacacaatgt taatacatta ataacattca ctttttaaaa
    15901 actggcatcc gctttgcaaa caagccatta cgcggctgaa aaggatatgc aggatgcagc
    15961 gccccgtctg actttcactt tacgagacga agagaggttg atgatgaaaa ttggcgtatt
    16021 cgtacctatt ggcaacaacg gctggctcat ttcgacccac gcgccgcagt acatgccgac
    16081 ctttgaactg aataaagcca ttgtgcaaaa agcggagcac taccatttcg atttcgccct
    16141 gtcgatgatc aaactgcgtg gctttggcgg caaaactgag ttctgggatc acaaccttga
    16201 gtcgttcacc ttgatggcgg ggctggcggc cgtcacctcg cgcattcaga tttacgccac
    16261 tgctgccacc ttaacgttac ctccggcaat cgtcgcccgt atggccgcaa ccatcgactc
    16321 catctctggc gggcgttttg gcgtcaacct cgtgactggc tggcaaaagc ccgagtatga
    16381 gcagatgggt atctggcctg gcgatgacta tttctcccgt cgttacgact atctcaccga
    16441 atatgttcag gtgctgcgcg acctgtgggg cacggggaaa agcgatttta aaggcgatta
    16501 tttcaccatg aatgattgtc gcgtcagtcc gcaaccgagt gtccccatga aagtgatctg
    16561 cgccgggcaa agcgacgctg gcatggcgtt ctccgcccag tatgccgatt tcaacttctg
    16621 tttcggcaaa ggcgtaaata cgcccacggc tttcgccccg accgctgcgc ggatgaaaca
    16681 ggccgcagag caaaccgggc gcgacgttgg ttcttatgtg ttgtttatgg tgattgccga
    16741 tgaaaccgac gatgccgctc gcgccaaatg ggaacactac aaagcgggcg cggatgaaga
    16801 ggcgttaagc tggctaaccg aacaaagtca gaaagatacc cgctccggta ctgataccaa
    16861 cgtccgtcag atggccgatc ccacttcggc ggtaaacatc aatatgggga cgttagtcgg
    16921 ttcttacgcc agtgtcgcgc gcatgttaga tgaagtcgca agcgtgcctg gtgccgaagg
    16981 cgtgctgttg accttcgatg attttctgtc gggaatcgaa accttcggcg agcgcattca
    17041 accactgatg cagtgccgcg cccatctacc tgcgctgact caggaggtgg catgatgaca
    17101 accttaaccg ctcgaccgga agccattacc ttcgatccgc agcaaagtgc gctgatcgtg
    17161 gtggatatgc aaaacgctta tgccacgcca ggcggctact tagatctcgc cgggtttgat
    17221 gtctcaacca ctcgcccggt cattgccaac attcaaaccg ccgtgaccgc agcgcgagcg
    17281 gcagggatgc tgatcatctg gtttcaaaat ggctgggatg aacagtatgt cgaagctggc
    17341 ggccccggct caccgaattt tcataaatcg aacgccctga aaaccatgcg taagcagccg
    17401 cagctgcagg ggaaattgct ggcgaaaggc tcctgggatt atcaactggt ggatgaactg
    17461 gtgccgcagc ctggcgatat tgtgctgccg aagccgcgct acagcggttt cttcaataca
    17521 ccgctggaca gcattttgcg cagccgtgga atacgccatc tggttttcac cggtatcgct
    17581 accaacgtct gcgtcgaatc gacgctacgc gatggctttt ttctggagta tttcggcgtg
    17641 gtgctggaag acgcaacaca ccaggcgggg ccggaatttg cacagaaagc cgcgttgttc
    17701 aatatcgaaa ccttttttgg ctgggtcagc gacgtcgaaa cgttctgcga cgcgctttct
    17761 cccacatcct ttgctcgtat cgcttaagga gtttaacgat gccaaaatcc gtaattattc
    17821 ccgctggcag cagcgcaccg ctggccccct tcgttcccgg cacgctggct gatggcgtgg
    17881 tgtatgtctc cggtacgctg gcttttgatc aacataataa cgtgctgttt gccgatgacc
    17941 caaaggcgca aacccgccac gttctggaaa ttatccgcaa ggtgatcgag acggcgggtg
    18001 gcacgatggc ggatgtgacc ttcaacagca tctttattac cgactggaaa aattacgccg
    18061 cgattaacga aatctacgcc gagttttttc cgggtgataa accggcgcga ttctgcattc
    18121 agtgcggact ggtaaaacct gacgcgctgg tggaaatcgc cacaattgcg catatcgcca
    18181 agtgaggccg cgatgaaact ttcactctca cctccccctt atgctgatgc gcccgtagtg
    18241 gtgttgattt cgggtcttgg cggtagcggc agttactggt taccgcaact ggcggtgctg
    18301 gagcaggagt atcaggtagt ctgttacgac cagcgcggca ccggcaataa tcccgacacg
    18361 ctggcagaag attacagtat cgcccagatg gcagcggaac tgcatcaggc gctggtagcc
    18421 gcagggattg agcattacgc ggtggtcggc catgcgctcg gtgcgctggt gggaatgcag
    18481 ctggcgctgg attatcccgc gtcggtaact gtgctgatca gcgttaacgg ctggctacga
    18541 ataaacgccc atacgcgccg ctgttttcag gttcgcgaac gattactgta tagcggcggc
    18601 gcgcaggcat gggtggaagc gcagccgttg ttcctctatc ccgccgactg gatggcggcc
    18661 cgcgcacctc gccttgaggc agaagacgcg ctggcactgg cgcattttca gggcaaaaat
    18721 aatttactgc gtcgacttaa cgccctcaaa cgcgctgact ttagtcacca tgcggatcgc
    18781 atccgctgcc cggtgcaaat catctgcgcc agtgatgatc tgctggtgcc atcagcatgt
    18841 tccagtgagc ttcatgccgc cctgcccgat agccagaaaa tggtgatgcg ctatggcgga
    18901 cacgcctgca acgtgaccga ccccgaaacg tttaatgctc tgttactcaa cgggcttgcc
    18961 agcctgttac atcaccgtga agccgccctg taaggaattg ctatgaacga agccgttagc
    19021 ccaggtgcgc ttagcacctt gttcaccgat gcccgcactc acaacggctg gcgggagaca
    19081 cccgtcagcg atgagacgtt acgggagatt tatgccctga tgaaatgggg gccgacatca
    19141 gctaactgtt ctccggcacg gatcgtgttt atccgcacgg cagaaggaaa agaacgtctg
    19201 cgcccggcac tttccagcgg caatctgcaa aaaaccctga ccgcgcccgt caccgctatc
    19261 gtcgcctggg acagtgaatt ttatgaacgg ttaccgcaac tgtttcctca cggcgatgcc
    19321 cgcagttggt ttacctccag cccacaactt gccgaagaaa cagcgtttcg caacagttcc
    19381 atgcaggcgg cctatctgat cgtcgcctgc cgtgcgctgg gactggatac cggcccgatg
    19441 tcgggttttg accgtcaaca cgttgacgac gccttttttg cgggcagcac gctgaagagc
    19501 aatctgctga ttaatatcgg ctatggcgat agcagcaaac tttttgcgcg cctgccacgt
    19561 ctgtcctttg aagaagcctg cgggctgttg taaggagcaa accatgaaca ttgtcgatca
    19621 acaaaccttt cgcgatgcga tgtcctgtat gggcgcggca gtcaatatca tcaccacgga
    19681 tggtccagcc gggcgcgccg ggttcaccgc cagcgcggtc tgcagcgtga ccgacacgcc
    19741 gcctacatta ctggtgtgcc tgaatcgtgg ggcgtccgtc tggccggtat tcaatgaaaa
    19801 tcgaacgctg tgtgtaaata cactgagcgc cgggcaggag ccgctttcaa atctttttgg
    19861 cggcaaaacg cccatggaac accgctttgc cgccgcccgc tggcagacgg gtgtgaccgg
    19921 atgcccgcaa ctggaagagg ctctggtttc gtttgactgc cgtatcagcc aggtggtgag
    19981 cgtcggcacc cacgacattc tgttttgcgc catcgaagcg attcatcgtc acgccacacc
    20041 ctacgggctg gtgtggttcg atcgcagtta tcacgcgctg atgcgccctg cttgttaacc
    20101 tccttaagga gacagctgat ggcaatgttc ggttttcctc actggcagtt aaaatcgacc
    20161 tctacagaga gcggcgtggt cgcgccgtat gaacgattgc cgtttgctca gacggcagta
    20221 atgggcgttc aacacgcggt ggcgatgttt ggcgcgacgg tgttaatgcc gattctgatg
    20281 gggctggatc ccaatctttc cattttaatg tcggggatcg gcacgttact gtttttcttt
    20341 attaccggcg ggcgcgtccc cagttatctc ggttccagcg cggcttttgt cggcgtggtg
    20401 attgccgcga cgggctttaa cggtcagggc attaacccga atatcagcat tgcgctgggc
    20461 gggattatcg cctgtgggct ggtttatacc gtcattggcc tggtggtgat gaaaattggt
    20521 acacgctgga ttgaacgact aatgccgccg gtggtgacgg gtgcggtggt gatggcgatt
    20581 ggcctgaact tagcgcctat cgcagtgaaa agtgtatcgg cttcggcctt tgatagctgg
    20641 atggcggtaa tgacggtgct gtgtattggg ctggtggcgg tgtttacccg tggcatgatc
    20701 cagcggttat tgattctcgt aggtttaatt gtcgcctgtc tgctgtatgg cgtgatgacc
    20761 aacgttttag ggctgggaaa agccgtcgat tttactctcg tcagccatgc tgcctggttc
    20821 ggtttgccac atttttccac tcctgctttt aatagtcagg caatgatgct gattgcgccc
    20881 gtggcggtga ttctggtggc ggaaaactta ggtcatctca aggcggtcgc cgggatgacc
    20941 ggacgcaata tggacccgta tatggggcgg gcattcgtcg gtgatgggct ggcgacgatg
    21001 ctttctggtt ctgtcggtgg cagcggcgta accacctacg ccgagaatat cggtgtaatg
    21061 gccgtgacaa aagtctactc gacgctggtg tttgtcgctg ctgccgtcat cgccatgctg
    21121 ttgggctttt caccgaaatt tggcgcgctg attcatacca ttccggccgc ggtgattggt
    21181 ggtgcatcaa ttgttgtgtt cgggctgatt gccgtcgcag gcgcaaggat ctgggtacaa
    21241 aaccgtgtcg atttaagcca gaacggtaat ttgattatgg tcgcagtaac cttagtgctg
    21301 ggcgcgggtg attttgctct cacgctgggc ggttttacgt tgggagggat cggtacagcc
    21361 acctttggcg cgattttgct caatgcgttg ttaagtagaa ggttggttga cgttccaccg
    21421 ccagaagttg ttcatcagga gccataaaca gaaaaccgcc ggggttaccc ggcggtgatg
    21481 actttttgac gagtctaaga aaaaaatgca ttcgtcattg attagcccgg ctagttgtcg
    21541 cttttgccgt gactgctctt gccacctttt ttacctgctt cagatgcgcg ctgcgggtca
    21601 ttcttgaaat tacccccgct gtgctgtcca ccttttttac ctgcttctga tgctctttcg
    21661 cggtcttctg caaaattgcc ggaaccgcct cgatggtttg ccattactga cctccgtctg
    21721 ggtatagaca tcatattgca tagaattaac gcggcgtatg gcggctgcca tattttcgct
    21781 acgtgggact aagcttagtg aactgacgcg ggcagacccg cgtttggtaa tatcctgcaa
    21841 cagcttctta gcgagaatag atatcatctc caataattat ccataagccg ctcttatgat
    21901 taaaaaaacg cgatgagata aaaatgtgtc attttgcgac aaaattacgt gcttgtgaaa
    21961 gttgttataa atcaaataag tggttgtgaa atttgcactc tgaaaaggac gtcttatctt
    22021 taaataagtg gtagcgaatc gctacggaat agagataaca cgaggagtgg ttagaaatgg
    22081 ctaaagttct ggtgctttat tattccatgt acggacatat tgaaacgatg gcacgcgcag
    22141 tcgctgaggg tgcaagcaaa gtggatggcg cagaagttgt cgttaagcgt gtaccggaaa
    22201 ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac gcaaactgca ccggttgcaa
    22261 ccccgcaaga actggccgat tacgacgcca ttatttttgg tacacctacc cgctttggca
    22321 acatgtccgg tcaaatgcgt accttcctcg accagacggg cggcctgtgg gcttccggcg
    22381 cactatacgg aaaactggcg agcgtcttta gttccaccgg tactggcggc ggtcaggaac
    22441 aaactattac ttcaacctgg acgacccttg cgcatcacgg catggtaatt gtccccattg
    22501 gctacgcagc gcaggaatta tttgacgttt cacaggttcg cggcggtacg ccgtacggcg
    22561 caaccaccat cgcaggcggt gacggctcac gccagccaag ccaggaagaa ctgtctattg
    22621 ctcgttatca aggggaatat gtcgcaggtc tggcagttaa acttaacggc taatcttcaa
    22681 caggaggata cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg aatgggcatc
    22741 tctgcgcaat acgtcgccgg aaatagccga ggccattttc gaagtcgccg ggtacgacga
    22801 aaaaatggcg gaaaagattt gggaagaagg tagcgatgaa gtgttagtca aagcctttgc
    22861 taagaccgat aaagattcgc ttttttgggg cgaacagacc atcgaacgta aaaacgttta
    22921 accagcgact cccccgcttc tcgcggggga gttttctgtt atttcaccgc ttcattcaac
    22981 acgctatcaa atttatccat cgggcagaaa ccgttggcat ctatcgggca accgcttaat
    23041 tccagcgtca cacgctgagc aggtgcctgc agggttaacg catcggcatt acgtaactgt
    23101 tccgcactct gatacacata ttcaattttc atcaaatcgc gattggcttt gctgtcatgc
    23161 caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc atgcaactga
    23221 tacggtttga aatccagcgc cgttaacaga gaggcaatgt tggagtcgtg cccaaccaac
    23281 actgtaattt tcggtgcgct ggtgcgatcg gtgaccagag ctttgtcgat ataactgacc
    23341 agcggtttcg caacattgcg cgccacttcc ggtgaggtaa acaggctgtc ctggtagccg
    23401 tttttaagct tcgacaacac cttccactgc tggtcagatt tgatttctcc ccaggccacc
    23461 tgatccatcg gaaaaccttc gtaatattgc aaagtaaacg catctaccag cgagttgccg
    23521 actttcagcg gcccggaaac acctggttct tgttgatact tggcgctaaa ggtatttttg
    23581 ccatccacca gcgaacactg ttgtttctct ttacaggcag gggaatcttt atagttaacg
    23641 attttttcca gtagctggta gctgtcggta agctggagtt tgctgagctc tttctccatt
    23701 gccgccaccg cctgttcact gaatgcggcg gaatcatcgg tgatcaccgg gttaaaggtt
    23761 gggtccatag tgcccatttt ttcctggtga tgcacaggaa tatcacaccc cgggaatgcg
    23821 ccggtaataa agaactgtgc ggtcgcaacg gtacgttgca gactattggc ataggcataa
    23881 acggtgtccg gcggcgggca ttcccccgat ttcaccatcc cctgctctgc cagccattca
    23941 cgcatgtaat ggcccatata cacttcgagc acgccacctt tggtggtgag ttgcccaccg
    24001 gggacgtccc attctggcca tttattcggc gtcgactgct ccagcacact gccattgttc
    24061 gccagcggcg cacgtaagtt atggcggctc atcatgaaca cttgctgtag ctgatagcct
    24121 tccggtacgg tttgtgcctg agcgtttgaa gcgagtaaaa ctatccctgc cacagctgcg
    24181 gcgattagcg ttttgttcat tcctggcacc tcttttgtta tcaatcacta aagagtgtga
    24241 cagaaatatg acttttttcc gggaacaggt tcgcaaaatg gcttgttgaa cgcctgtttt
    24301 tctatataca tcttcaaaaa caggcaaggt ttgatgcaag ctatgtctgc ttatcccctg
    24361 cctcgcgtac accgctgatg attacacctg tggctaaaca ggatatcagc ggcgttacta
    24421 atacagaggc aatagtacca ataataagag aagtgaggct aataacaatg gtttttagca
    24481 catg
//
LOCUS       DAJSKJ010000048        23996 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000048 DAJSKJ010000000
VERSION     DAJSKJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.048"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..190)
                     /locus_tag="ON345_001635"
     CDS             complement(<1..190)
                     /locus_tag="ON345_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071715.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSE"
     gene            complement(290..1201)
                     /gene="gsiD"
                     /locus_tag="ON345_001636"
     CDS             complement(290..1201)
                     /gene="gsiD"
                     /locus_tag="ON345_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR3071716.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1204..2124)
                     /gene="gsiC"
                     /locus_tag="ON345_001637"
     CDS             complement(1204..2124)
                     /gene="gsiC"
                     /locus_tag="ON345_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR3071717.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2142..3680)
                     /gene="gsiB"
                     /locus_tag="ON345_001638"
     CDS             complement(2142..3680)
                     /gene="gsiB"
                     /locus_tag="ON345_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR3071718.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3700..5571)
                     /gene="gsiA"
                     /locus_tag="ON345_001639"
     CDS             complement(3700..5571)
                     /gene="gsiA"
                     /locus_tag="ON345_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR3071719.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5558..6523)
                     /gene="iaaA"
                     /locus_tag="ON345_001640"
     CDS             complement(5558..6523)
                     /gene="iaaA"
                     /locus_tag="ON345_001640"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3071720.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6727..7962
                     /gene="moeA"
                     /locus_tag="ON345_001641"
     CDS             6727..7962
                     /gene="moeA"
                     /locus_tag="ON345_001641"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR3071721.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7962..8711
                     /gene="moeB"
                     /locus_tag="ON345_001642"
     CDS             7962..8711
                     /gene="moeB"
                     /locus_tag="ON345_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR3071722.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8890..9552)
                     /gene="fsa"
                     /locus_tag="ON345_001643"
     CDS             complement(8890..9552)
                     /gene="fsa"
                     /locus_tag="ON345_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR3071723.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9683..10582
                     /locus_tag="ON345_001644"
     CDS             9683..10582
                     /locus_tag="ON345_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR3071724.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            10588..13020
                     /locus_tag="ON345_001645"
     CDS             10588..13020
                     /locus_tag="ON345_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR3071725.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATATRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13166..13981
                     /gene="ybiV"
                     /locus_tag="ON345_001646"
     CDS             13166..13981
                     /gene="ybiV"
                     /locus_tag="ON345_001646"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR3071726.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKCWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14133..15398
                     /locus_tag="ON345_001647"
     CDS             14133..15398
                     /locus_tag="ON345_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR3071727.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15639..17231)
                     /locus_tag="ON345_001648"
     CDS             complement(15639..17231)
                     /locus_tag="ON345_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR3071728.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17450..18370
                     /gene="ldtB"
                     /locus_tag="ON345_001649"
     CDS             17450..18370
                     /gene="ldtB"
                     /locus_tag="ON345_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR3071729.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18429..19547)
                     /locus_tag="ON345_001650"
     CDS             complement(18429..19547)
                     /locus_tag="ON345_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR3071730.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19544..20011)
                     /gene="mntR"
                     /locus_tag="ON345_001651"
     CDS             complement(19544..20011)
                     /gene="mntR"
                     /locus_tag="ON345_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR3071731.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20197..20325
                     /gene="mntS"
                     /locus_tag="ON345_001652"
     CDS             20197..20325
                     /gene="mntS"
                     /locus_tag="ON345_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR3071732.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20597..22180
                     /gene="opgE"
                     /locus_tag="ON345_001653"
     CDS             20597..22180
                     /gene="opgE"
                     /locus_tag="ON345_001653"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR3071733.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22229..22744)
                     /gene="ompX"
                     /locus_tag="ON345_001654"
     CDS             complement(22229..22744)
                     /gene="ompX"
                     /locus_tag="ON345_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR3071734.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22797..22862)
                     /locus_tag="ON345_001655"
     CDS             complement(22797..22862)
                     /locus_tag="ON345_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071735.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23097..23984
                     /gene="rhtA"
                     /locus_tag="ON345_001656"
     CDS             23097..23984
                     /gene="rhtA"
                     /locus_tag="ON345_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR3071736.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLHFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5839 a         6546 c         5991 g         5620 t
ORIGIN      
        1 gttccgatac cagttgatag ttaatgatga aaaaaagacc aataaatgac agtgctgcca
       61 ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc atgttaatac
      121 atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct tgctgcggct
      181 ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact tgcttagggc
      241 aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac tatcctttaa
      301 ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc agtacggtca
      361 gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata tccgctcgtg
      421 cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg ccgagaaatg
      481 acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac accacgatag
      541 acgagacggt cccaggcaag atatgacgca ataaaatggt catatcgctg gcaccaatac
      601 tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg cgcaccagtc
      661 gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca atgccgcttc
      721 ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac agcacatcgc
      781 aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac aagcccagca
      841 acgtcccgat cgccgcaccg ataaacacgg caaacacgcc cgccgccagc gagatttgcg
      901 caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca aaccagtgct
      961 gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg tcatagggag
     1021 cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat gcggcggtca
     1081 tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta cgcacctggt
     1141 caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta aatagtcgca
     1201 tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca ccactaagtt
     1261 gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg ggtaatcgcg
     1321 catttctacg gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa ctttctccac
     1381 cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca ctaccgggat
     1441 catcgcgtta cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc ctttcgccct
     1501 cgcggtacgc atataatctt cgcttaaaac atcgacaaac gacgcgcggg taaagcgcgc
     1561 catcacggct gccactgccg cgccgagcgt cagggagggt aaaatgtagt gctgccaact
     1621 gtctgctccc acggtaggca gccagcccag ttcaacggag aatacctgaa ttaaaagcat
     1681 ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac tcaatcgatc
     1741 cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa atatcaccgc
     1801 ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg caatctcatc
     1861 ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag cattgctgat
     1921 atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac gcaccagttc
     1981 tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc cgggcagcat
     2041 atgcacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca accccagtaa
     2101 gcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa atccgcgtct
     2161 tcaaagctga agccggtgtc tggcatgatc caaaaaccgg tcaggttttt actgtgtgcc
     2221 gacaccagtt tttctaccac cagcgggatc cacggagact cttgccagat gatatcctgc
     2281 gccgccttat acaagcgggt cttttccgcc ggatcattag ttttcagtgc ctgagccagg
     2341 aagtcgtcca cctgtttatt gctgtaaaat gcggtattaa acagcgtcgg tggccagttt
     2401 tgcgaggcga acagtggcga tagcgcccag tccgcttcgc cggttgaagc cgaccagcca
     2461 gtgtagaaca tccgcacgcc gctctctttt tgccctttac cttcaacttc tgccgcccgc
     2521 tgtccggcat ccatcgcagt cacctggact ttaatcccga cctgcgctaa ctgctgctgg
     2581 gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag cgtggtactg
     2641 aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg atcgtaaggc
     2701 cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt agctggcgtt
     2761 gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta attcagcgcc
     2821 tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat ataacgctgc
     2881 ataattgacg gactggccat caactcgata tttttgtttt tctccagcag tgtggcctgc
     2941 tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc cgcgcgggtg
     3001 ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc tggctgccag
     3061 taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc cagttcatac
     3121 ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag cgctgccggt
     3181 gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa cggctgtttg
     3241 agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt cttatacagg
     3301 ttatagcgtt taagatgatt cgccggatcg ctggctcggt ccagattcgc ttttaccgcc
     3361 actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt cacggtgtaa
     3421 gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag tttcatctct
     3481 ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga taacgtgtca
     3541 ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac cacatctttg
     3601 gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac taacccacta
     3661 cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca tgaatgcgta
     3721 ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg agacggctgc
     3781 cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca gtacacgctg
     3841 cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac gcgtataagg
     3901 atgctgcggg ttttcgaaga ccgcgcgccg tggaccaatt tcaacaattt gcccgagata
     3961 catcaccgcc acacgatgac taatccgctc taccacggcc atatcgtggg agataaacag
     4021 atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct gcccgcgaat
     4081 agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat tcaatgccaa
     4141 cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg ggtaacgcca
     4201 ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg cagccgcgtc
     4261 tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac cgatggtctg
     4321 acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc ggcgcaatgc
     4381 ctgaagtttg ccgggtgaca aggtatcgat tctctgaccg ttaaagataa tttcgccgcc
     4441 ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc tgccagactc
     4501 acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct caacggcatg
     4561 cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg tgaccagatt
     4621 acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg ggggttcctg
     4681 tttcgctgga tgttcaagcg atatcaacgg gaaacgtcgg ggataatcta accctttcat
     4741 cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt gcggtgcatg
     4801 aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca ccagtacccg
     4861 atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc ccatcgacat
     4921 ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga catccagcgc
     4981 ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca tcgcaatcat
     5041 cactcgctgg cgcatcccgc cagagagttg atgcggataa cgtgaaagaa tggtttgtgc
     5101 ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt cttcacgact
     5161 ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa ataccgggtt
     5221 cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca catggcgcat
     5281 ttgtgcagcg ctctgctcgc taagttcaat cacttcgcga ctgcgccgct gcaacagcat
     5341 tttatcgcac tgtactaaac cgcccgcctg ttccaacagg cgcatcaatg ccaacgcagt
     5401 cactgactta ccggagccgg attcgccaac aattgccagc gtctcaccgc gttgcagact
     5461 aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg caatattcag
     5521 attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca acggtgtccc
     5581 ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag gcgcgataca
     5641 ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg attaagccac
     5701 cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct tccgcgagac
     5761 ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg cggatgaaga
     5821 cttcgcccgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag catccggcac
     5881 ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg cctgtggacg
     5941 tggctgccgc caaattgccg tctaaatcca acgccacggc ccccacggtg cccattttct
     6001 gtttttcatc cagtggcgca ccgctatggt cgaggacggt tgccccttcc ttgcgcgctg
     6061 ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggcgagacg cgctccatgc
     6121 cacgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc gggctttgct
     6181 ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc gccaccgcac
     6241 cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt tcatcacgcg
     6301 taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga cgcaccgctt
     6361 ccgtcaccac atccagcgca ctttcgcccg cttccagcat tttctgcccg gtttcaacaa
     6421 tggcagacaa cgcctcaatg tagcgtaatt cctgttgcag actcatctgc gcgcggctaa
     6481 ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc gtccattaag
     6541 ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca ttcaattttt
     6601 gaatatagaa cgatggatat ggcatgtaaa ggcaggagcc aacgctacgc ccgaggtggc
     6661 atacagtctg tggcgttttt ctgacataat aggcaaattc gattttgcct ccgcaggagt
     6721 gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt
     6781 tctcgcgtca ccccactgac cgcccaggaa acgctgccac tggtacagtg ttttggtcgt
     6841 attctggcga gcgatgtcgt ttcgccgctt gatgttccgg ggtttgataa ctccgcaatg
     6901 gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc cgttgccggt
     6961 aaatcctttg ccggtcagcc gtatcatggt gaatggcctg cgggtacctg cattcgtatt
     7021 atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa
     7081 caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa tattcgccgt
     7141 cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg cctgactacc
     7201 gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat tcgtaaagtg
     7261 cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc gctgggcgac
     7321 ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca gctgggttgc
     7381 gaggtaatca acttagggat tatccgcgac gatccccatg ccctgcgcgc cgcatttatt
     7441 gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt gggtgaagcg
     7501 gattacacca aaacgattct tgaagagctg ggggagatcg ccttctggaa gctggcgatt
     7561 aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg cggcctgccg
     7621 ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt gctggcaaaa
     7681 ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc
     7741 ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg caacgccgac
     7801 ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag ctcctttagt
     7861 ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt gggcgaatgg
     7921 gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa ctcagcgatc
     7981 aggagatgct gcgctataac cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg
     8041 aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc tgcgcagctt
     8101 cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc gacacggttt
     8161 cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg caaccgaagg
     8221 tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt acgccagtca
     8281 atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat ctggtgctcg
     8341 actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt gccgcgaagg
     8401 tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc ttcacttatc
     8461 aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac gcattaacct
     8521 gcgtagaagc aggcgtgatg gcaccgttga tcggcgtaat tggttcgttg caggcaatgg
     8581 aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc gtcatgtacg
     8641 atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg tgtgaggtgt
     8701 gcgggcagta attgccacct ccaggcctga taagacgcga caagcgtcgc atcaggcatt
     8761 tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg ggcacagatt
     8821 tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat gaggatgcag
     8881 aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct caaacttcgc
     8941 cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca gtggcagagt
     9001 aattgattca catcctgcca gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc
     9061 tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt cggtcacagt
     9121 ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa catattccgc
     9181 acctgccagc gccgacagca gcccttgtgc tgcgccatat accgcggttc ccagcgttgg
     9241 aatcccttcc gcttttaaca tcttaatagc tgccagcccc tcggcggtca ccggaacttt
     9301 caccacgata tccgcaataa tagaacgcaa tttacgtgcg tcattcacca tcccttcagc
     9361 agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt catgaagttg
     9421 cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt tagtggtcac
     9481 acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg aagtatccag
     9541 atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac tatcacggga
     9601 aaactctctg aacagttgac gcacatcaat ctaactttca ttcgaaagta ttttaatgtt
     9661 tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga cccatgatgg
     9721 ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc gctggtgtca
     9781 gaacccggaa agccgcgccc gcacgcagga tctgctgtat gacgcacgac tgtgtctgga
     9841 aggctgcgag ctgtgcgcta aggcagcgcc ggaagtgatt gagcgcgcat tgaatggttt
     9901 gcttattcat cgggaaaagt taaccccgga gcacctgacg gcgctaaccg actgctgccc
     9961 gacacaggcg ttaaccgtgt gtggggaagt gaaaagcgtt gaggagatca tggcgaccgt
    10021 tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt cgggtggtga
    10081 accctttatg cagccggaaa tggcgacagc gctactgcaa gccagccacg aggccggcat
    10141 tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc cttctctgcc
    10201 ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg
    10261 gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg cagcgggcaa
    10321 aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa cctctgtaaa
    10381 agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt ttctgcccta
    10441 ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg ccccggaaaa
    10501 accgcttgat gcgccagacc tgctcgactt tgcccagcag tatgcctgcc agaaagggtt
    10561 aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac gctcagcgac
    10621 cgcattaaag cgcacaaaaa tgcgctggtg catattgtga aaccgccggt ctgtaccgag
    10681 cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat cccggtacgt
    10741 cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa acacgatgag
    10801 ttgatcatcg gcaaccaggc aagcgaggtt cgcgccgcgc cgatcttccc ggaatatacc
    10861 gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc tggttttgcg
    10921 gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc cgtggtggcg cggtcagacg
    10981 gtacaggatc gctgctacgg catgtttacc gatgagcaaa aaggtctgct ggcgaccggc
    11041 atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt gaatttcccg
    11101 ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg tcgctcgcgc
    11161 atcaatctga cggtactgga agatttgcac ggcgaacagt tcctgaaagc gattgatatc
    11221 gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg tgcaatggcc
    11281 gcgaccgcaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga aaactgcgat
    11341 cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg ttacttcatc
    11401 cagttgatat tgcagatcga atctaacggt cactcggtat cgtttggtcg tatggaccag
    11461 tatctctacc cgtactatcg ccgcgacgtt gaactcaacc agacactgga tcgcgaacac
    11521 gccatcgaat tgctgcatag ctgctggctg aaactgctgg aagtgaacaa aatccgctca
    11581 ggctcacact caaaagcctc tgcgggaagt cctctgtatc agaacgtcac cattggcgga
    11641 caaactctgg ttgatggtca accaatggac gcggtgaatc cactctctta cgcgatcctc
    11701 gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta ccatgcagga
    11761 atgagtaatg atttcctcga cgcctgcgta caggtgatcc gttgcggctt cgggatgccg
    11821 gcgttcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat tgaaccgcag
    11881 gacgcttacg actacgcagc gattggttgt atcgaaaccg ccgtcggtgg caaatggggc
    11941 tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa
    12001 ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc gttgtcggca
    12061 ggcaacttta acaatttcga tgaagtaatg gacgcgtggg acacgcaaat ccgttattac
    12121 acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga gaacgtgcac
    12181 gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag tatcaagcaa
    12241 ggcggcgcga aatatgactg ggtttctggc ctgcaggttg gcattgccaa cctcggcaac
    12301 agcctggcgg cagtgaagaa actggtattt gaacagggcg cgattggtca gcaacagctg
    12361 gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg tcagcggctg
    12421 attaacggtg cgccgaagta cggcaacgac gatgatactg tcgatacgct gctggctcgc
    12481 gcttatcaga cctatatcga cgaactgaaa cagtaccata atccgcgcta cggtcgtggt
    12541 ccggttggcg gcaactatta cgcgggtacg tcatctatct ccgctaacgt accgtttggc
    12601 gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc agaaggcgct
    12661 agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc ggtgggtaaa
    12721 ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa tccggcaacg
    12781 ctggagaacg aatctgacaa gcagaagctg atgatcctgc tgcgcacctt cttcgaggtg
    12841 cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct ggaggcgaaa
    12901 aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc cgccttcttc
    12961 accgcgctct ctccagacgc tcaggacgat atcatcgctc gtactgaaca tatgctgtaa
    13021 tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca atttcatcct
    13081 tactttcatt cgaaatataa tttatgctct gcgtcacatt gttattacac tgttacggtt
    13141 tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag acatggacgg
    13201 tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc aatatcagga
    13261 actgaaaaag cgcggcattg agttcgttgt cgccagcggt aatcagtatt accagcttat
    13321 ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg gcgcactggt
    13381 ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat cgcggattgt
    13441 tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc tgcaaagtgc
    13501 atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac actaccatcg
    13561 cctgaaagct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt tttcgctcaa
    13621 cctgccagat gaacaaatcc cgttagtgat cgacaaactg cacatagcgc tcgatggcat
    13681 tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg gtctacataa
    13741 agcaaacggt atttcgcggt tactgaaatg ctgggatctg tcaccgcaaa atgtggtagc
    13801 gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt cctttgcgat
    13861 gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg ataataatca
    13921 tgaaggcgcg ctgaatgtga ttcaggcggt gctggataac acatcccctt ttaacagctg
    13981 acctctctac cggagccgtg cgctccggtt ttcccttctt tttacatcct gtacatcaca
    14041 tcaaatcatt aaacttgcat taacttattt ataacttata ttatcacatg caaatcagaa
    14101 tggtttcgaa tgaaagttaa tgagaggtgg ttatggcttc tacttttacc agcgacacat
    14161 tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg
    14221 gcgacgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga gtggctgaaa
    14281 tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat gccgatatca
    14341 aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga
    14401 taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg ctggactatc
    14461 tggaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc ttcgggacgc
    14521 tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa atgcaggccc
    14581 gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa
    14641 gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac cgtatccgcc
    14701 gccgtccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactct ggggcgctgg
    14761 aacgctggct gcttccagcg tatcagcgcg ttttcgccaa cgtctttaat ggcaatctgg
    14821 cgcaatatga tccctggcat gcggcacatc gtacggaagt tgaagagtac acggtggaca
    14881 acaccaccaa atgttccgtg tttcggacat tccagggctg gacagcgctc tctgatatgc
    14941 tgcctggtca ggggttgctg cacgttgtgc ccattcctga agccatggcg tacgtactgt
    15001 tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg cccggaagag
    15061 tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc agcattccaa
    15121 aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc gttgcccccg
    15181 ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga
    15241 aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg
    15301 acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt gccgacctca
    15361 acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa gtgaattgat
    15421 gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg ctttcttaat
    15481 gcctgatgcg gcgttaacgc cttatctggc ctacaaagtc ctgcagattc aatatattac
    15541 aggtactgcg taggcctgat aagcgtagcg catcaggcta ttttgcattt gtcatcagcc
    15601 gccgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc tttactacgc
    15661 aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat ttccagaatg
    15721 cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag cgtgccctga
    15781 tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg gttggtcggt
    15841 tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag catccgccct
    15901 ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct gaacagcaaa
    15961 cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca ctgactcatc
    16021 cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc gtagtaacca
    16081 atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc gcccaccagc
    16141 gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag tttttcaccg
    16201 acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc tttggtcaga
    16261 ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg gatgaacggg
    16321 ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc
    16381 gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac
    16441 tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg cgcctgggtc
    16501 gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc gtaatccaga
    16561 tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat gatgatcatg
    16621 gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg
    16681 ttggtcggtt cgtcgagcag gagaatatcc ggatcagcaa acagcgcctg cgccagaagc
    16741 acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg ttccactgga
    16801 attcccacgc caagcaacaa ttcaccggcg cgagcttccg cagagtaacc gtccatttcg
    16861 ccgtatttaa cttccaggtc ggccacttta tagccgtctt cctcactcat ttccggcaaa
    16921 gcatagatgc ggtcacgctc ctgcttcact tcccacaact ctttatgccc catgatcacc
    16981 gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt accaatgcgc
    17041 tcgttgggat cgagggaaac gttacccaaa gtcggctcta agtcgccgcc gaggatcttc
    17101 ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt gccgccgcca
    17161 aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt gacgttactg
    17221 gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta tgccataagt
    17281 ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc aaaaaatgtg
    17341 atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg cttccaatac
    17401 tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa
    17461 attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc
    17521 ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac
    17581 cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc agatggggct
    17641 ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag gcggtactgt
    17701 actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca tcgtcattaa
    17761 cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta tcgtgctgcc
    17821 gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca aagttgagcg
    17881 taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt accgcgctgc
    17941 gggcgaaccg cttccggctg tcgttccggc aggtccggat aacccgatgg ggctgtatgc
    18001 actctacatc ggtcgcctgt acgctatcca tggcaccaac gccaacttcg gtatcggcct
    18061 gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc tgttcgagaa
    18121 agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga ccaccgagcc
    18181 agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag cccagtttga
    18241 aggtcaggaa attgtgccaa ttaccctgac gaagagcgtg caaacagtga ccggtcagcc
    18301 agatgttgac caggttgttc ttgatgaagc gattaaaaac cgctccggga tgccggttcg
    18361 tctgaattaa tcttcaaaac ttaaagcaaa aggcggactc ataatccgcc ttttttattt
    18421 gccagaccct agttggccgg gagtataact aacaaaacat atcccaccaa cgccgcccac
    18481 aacagcatcg gtattgaata gagatggaaa cgccaccaga tgcggcgatc gttcgccata
    18541 cgtagcgcaa tcaaatttgc cagcgatccg ggtaataacc caaagccacc tacgtttacc
    18601 gcccatacca gtaataaaga cggcggcaca tagttcagca acaatatggt actcggcacg
    18661 ttactgatca cctgcgataa accgattgcc gttaaccata acccgggttc agatagatga
    18721 ctcacgttac ccaacacgcc ttgcaacgct ggaagctggg tcagtaaatg gacgtcgata
    18781 aacatcgcca taaacaccag cagcagcgtc cagtccacac tgagcaccac gcgacgtgcc
    18841 agcagcgcaa agcctgccgc cacaatcacc agtccccaca gctcttgttt gaactccagc
    18901 gccgtcagaa agacgatata cagccccaga caactccaca ccagccgcgg tttccactcc
    18961 ggtgtttgca cccccgtatg gtattgcagc gcctttccag ggaaacaaca ccagcacagg
    19021 agcaggagcg tcagcatcat tgcgccagcc agcggtgcca tttgggcaat aaatccggca
    19081 aacgaaagac cagaacgtcc ccagataaga atattttgcg ggttgccaat tggcgtcagt
    19141 agcgaaccag cgttgactgc cagcgcctca aaaataatca gccgattaac cgggatctca
    19201 cacagtcttt ttagcgtgat agtcagcgga acaacaataa acagcgcgac atcgttggtc
    19261 agaaaggtag aaagcagcgc cgccgccagc accataaaca tcgccagccg acgctccgta
    19321 gcaaagcggc gcaccatttt gcgccccagc acatcaaaat aaccgcttaa ctccacacct
    19381 ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt gccagtcgat agcagcaggc
    19441 caggatttcg gtgcaaaggg cacgaaaaag cttaatccga taccaacaag aattaataac
    19501 tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca ggctcatttg gcaccgtgtt
    19561 tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact aacatggtgc tccatgcctt
    19621 ccgcgtcgcg acgggcgatt tccggactga cgcccaacac caacaagaaa ttttcgacta
    19681 tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc ttctgccgtt aaaaacacgc
    19741 cacgccaggg gatcatttca atcagcccca tggttgccag ccgcttaagc attttagcca
    19801 ccgtcggttg cgaaactccc agacgagcag ccatgtccac ctgacgagct tcccccactt
    19861 ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat aagctcgcgc cgatgcgcct
    19921 ctctgacctg gcggaaccct tcaacgtgct cttccacgtt cactaactgc gtcacttttt
    19981 ttgctgttgg cgtacctgcg cgacgactca ttgtgcttcc tcattatggt gacgcataca
    20041 tagcgttctc atttcgtgcg cacattgtaa accagagttg cgaaggtaca aaaaattaac
    20101 gttttagcaa tagctatata atatagcctg tgctatatct gtatgtaatg caatcatccc
    20161 tcaaggatcg acgggattag caagtcagga ggtcttatga atgagttcaa gaggtgtatg
    20221 cgcgtgttta gtcattctcc ctttaaagta cggttaatgc tgctctctat gttgtgcgat
    20281 atggtcaaca acaaaccgca gcaagataaa ccttccgata aatagcggcg tcgcggtacg
    20341 ccgcttcact cctgctttca tgcaggcata acgcgttttg gtctgaaaaa ccccactttt
    20401 tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc agtagttgac ctgaacggcg
    20461 gctcgctcta tcttcttgca gccctgcgta tattgcggct cgcggatgcg gacccctttc
    20521 cactcttcac gcactcttgc aggtattgac ccttgacgcc agggtaagca catggcgttt
    20581 gttacgatag tggcatatga atttaaccct caaagaatcg cttgttaccc gtagccgggt
    20641 atttagcccg tggactgcgt tctacttttt acagtcgcta ttaattaacc tcggcttagg
    20701 ttaccccttc agtttgctct acaccgctgc gtttacggct attttgcttt tgctatggcg
    20761 aacattgcct cgcgtacaaa aagttctggt cggtgtcagt tcgctggtgg cggcttgtta
    20821 tttccctttt gctcaggcct acggcgcgcc taatttcaat acattgctgg cattgcactc
    20881 caccaatatg gaagagtcga ccgaaatcct gacgattttt ccgtggtaca gctacctggt
    20941 cggcttattt atttttgcgc tcggcgtaat agcaatcagg cgaaaaaaag agaatgaaaa
    21001 agcgcgctgg aataccttcg acagcctgtg tctggtattc agtgtggcga cattttttgt
    21061 tgctcccgtg caaaacctgg cctggggtgg cgtatttaaa ctgaaagata ctggctatcc
    21121 ggtatttcgt tttgctaagg atgtcatcgt caataataac gaggtgattg aagagcaaga
    21181 acggatggca aaactttccg gaatgaaaga tacctggacg gtcactgccg ttaagccgaa
    21241 gtatcagacc tatgtggtgg tgatcggtga aagcgcgcgt cgcgatgccc tcggtgcctt
    21301 tggcggtcac tgggacaata ccccgtttgc cagcagcgtt aacggtttga tatttgctga
    21361 ctacattgcc gccagtggct ccacgcagaa atcgcttggc ttaacgctca atcgcgtagt
    21421 cgatggcaaa ccacagtttc aggataactt tgtcaccctg gcaaatcgcg cgggcttcca
    21481 gacctggtgg ttttccaacc agggccaaat cggcgaatac gatactgcta tcgccagcat
    21541 cgccaaacgt gcagatgaag tgtacttcct gaaagaaggt aattttgaag cagataaaaa
    21601 cacgaaagac gaagcgttac tggatatgac cgctcaagtg ctggcgcaag agcactcgca
    21661 accgcagctg attgttctgc atctgatggg ctcgcatccg caggcctgcg acaggacaca
    21721 aggcaaatac gaaacctttg tgcaatcgaa agaaacgtcg tgctatctct ataccatgac
    21781 gcaaacggac gatttactgc gcaagctgta cgatcagtta cgcaacagcg gcagcagctt
    21841 ctctctggtt tacttttctg accacggtct ggcctttaaa gagcgcggca aagacgtgca
    21901 ataccttgcc catgatgata aatatcagca aaatttccag gtgcctttta tggtcatttc
    21961 cagcgacgat aaagcgcatc gggtgattaa agcccgccgc tcagccaatg acttcttagg
    22021 ctttttctcg cagtggacgg gaattaaagc gaaggaaata aatatcaaat acccgtttat
    22081 atctgagaag aaagccgggc cgatatacat caccaacttc cagttacaga aggtggatta
    22141 caaccatctc ggaaccgata ttttcgaccc gaaaccttaa aacaaaaaat ccgccccgag
    22201 aggcggattt tttatatcac caaagtgatt agaagcggta accaacaccg gcaatccagg
    22261 tacctacgtc aacgctacga atacggctct gctcgtaaga gaagtccaga gcaacgtttt
    22321 ccatcgggtt gaactgcaga ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt
    22381 tgtaggtcgg gtattcagtg gtctggaatt taccataacc cacccccact acaccgtaga
    22441 tgcttgccca gtcgttaatg cggtaagccg gaccagcagt gatgccgtag tactggtttt
    22501 tgttgtagtc accagagctt gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca
    22561 cacccagcgg gctgttgtct tcttcatagc ggtatttcag gttgaaaccg cccattttgt
    22621 tcatttggcc ctgagcgtcg ctctgtgcgt aaccgccagt tacggtagaa gtcgcagcta
    22681 cggaagtacc tgcggtgaaa gccagaactg cggccagtgc tgaaagacat gcaatttttt
    22741 tcataaccac ctcaaatgtg attcaaataa gtcctaagtt ttaaatatat caaaaattaa
    22801 tgggaaactc ttcgcgattt gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc
    22861 atacaccgct atccatctaa atttaaatca ctttttcaga gaactgcgta agtattacgc
    22921 atgttttccc tgtcattcat ccagattatt cctaatcacc agactaatga ttccatcaat
    22981 cctggcgcat tttagtcaaa acgggggaaa attttttcaa caaatgctca accagcattg
    23041 ggtatatcca gtacactcca cgctttactt aagtctagat atttgtggga gaaaggatgc
    23101 ctggttcatt acgtaaaatg ccggtctggt taccaataat catattgctc gttgccatgg
    23161 cgtctattca gggtggagcc tcgttagcta agtcactttt tcctctggtg ggcgcaccgg
    23221 gtgtcactgc gctgcgtctg gcattaggaa cgctgatcct catcgcgttc tttaagccat
    23281 ggcgactgca ctttgccaaa gagcaacggt taccgctgtt gttttacggc gtttcgctgg
    23341 gtgggatgaa ttatcttttt tatctttcta ttcagacagt accgctgggt attgcggtgg
    23401 cgctggagtt caccggacca ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt
    23461 tcgtctgggt tgtgctggcg gttcttggtc tgtggttcct gctaccgctg gggcaagacg
    23521 tttcccatgt cgatttaacc ggctgtgcgc tggcactggg ggccggggct tgttgggcta
    23581 tttacatttt aagtgggcaa cgcgcaggag cggaacatgg ccctgcgacg gtggcaattg
    23641 gttcgttgat tgcagcgtta attttcgtgc caattggagc gcttcaggct ggtgaagcac
    23701 tctggcactg gtcggttatt ccattgggtc tggctgtcgc tattctctcg accgctctgc
    23761 cttattcgct ggaaatgatt gccctcaccc gtttgccaac acggacattt ggtacgctga
    23821 tgagcatgga accggcgctg gctgccgttt ccgggatgat tttcctcgga gaaacactga
    23881 cacccataca gctgctggcg ctcggcgcta tcatcgccgc ttcaatgggg tctacgctga
    23941 cagtacgcaa agagagcaaa ataaaagaat tagacattaa ttaaatttac atttct
//
LOCUS       DAJSKJ010000049        23975 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000049 DAJSKJ010000000
VERSION     DAJSKJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.049"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..515
                     /locus_tag="ON345_001657"
     CDS             <1..515
                     /locus_tag="ON345_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3071737.1"
                     /translation="LNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPF
                     DISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLM
                     TLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFK
                     ATSTFQVTYP"
     gene            628..1422
                     /gene="panB"
                     /locus_tag="ON345_001658"
     CDS             628..1422
                     /gene="panB"
                     /locus_tag="ON345_001658"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR3071738.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1434..2285
                     /gene="panC"
                     /locus_tag="ON345_001659"
     CDS             1434..2285
                     /gene="panC"
                     /locus_tag="ON345_001659"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR3071739.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2359..3261)
                     /gene="rpnC"
                     /locus_tag="ON345_001660"
     CDS             complement(2359..3261)
                     /gene="rpnC"
                     /locus_tag="ON345_001660"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR3071740.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQSEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3535..3915
                     /gene="panD"
                     /locus_tag="ON345_001661"
     CDS             3535..3915
                     /gene="panD"
                     /locus_tag="ON345_001661"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR3071741.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3919..5148)
                     /locus_tag="ON345_001662"
     CDS             complement(3919..5148)
                     /locus_tag="ON345_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3071742.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSHIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5212..5652)
                     /locus_tag="ON345_001663"
     CDS             complement(5212..5652)
                     /locus_tag="ON345_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3071743.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5757..6527)
                     /locus_tag="ON345_001664"
     CDS             complement(5757..6527)
                     /locus_tag="ON345_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3071744.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6524..7450)
                     /locus_tag="ON345_001665"
     CDS             complement(6524..7450)
                     /locus_tag="ON345_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3071745.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7559..8221
                     /gene="can"
                     /locus_tag="ON345_001666"
     CDS             7559..8221
                     /gene="can"
                     /locus_tag="ON345_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR3071746.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8262..8798)
                     /gene="hpt"
                     /locus_tag="ON345_001667"
     CDS             complement(8262..8798)
                     /gene="hpt"
                     /locus_tag="ON345_001667"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR3071747.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            9004..11394
                     /gene="gcd"
                     /locus_tag="ON345_001668"
     CDS             9004..11394
                     /gene="gcd"
                     /locus_tag="ON345_001668"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR3071748.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11596..13146)
                     /gene="cueO"
                     /locus_tag="ON345_001669"
     CDS             complement(11596..13146)
                     /gene="cueO"
                     /locus_tag="ON345_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR3071749.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13312..13659
                     /locus_tag="ON345_001670"
     CDS             13312..13659
                     /locus_tag="ON345_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR3071750.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13765..14631
                     /gene="speE"
                     /locus_tag="ON345_001671"
     CDS             13765..14631
                     /gene="speE"
                     /locus_tag="ON345_001671"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR3071751.1"
                     /translation="MAEKKHWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14647..15441
                     /gene="speD"
                     /locus_tag="ON345_001672"
     CDS             14647..15441
                     /gene="speD"
                     /locus_tag="ON345_001672"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR3071752.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15479..15841)
                     /locus_tag="ON345_001673"
     CDS             complement(15479..15841)
                     /locus_tag="ON345_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR3071753.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16016..18613)
                     /gene="acnB"
                     /locus_tag="ON345_001674"
     CDS             complement(16016..18613)
                     /gene="acnB"
                     /locus_tag="ON345_001674"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR3071754.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGCGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLCPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18968..20725
                     /locus_tag="ON345_001675"
     CDS             18968..20725
                     /locus_tag="ON345_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR3071755.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQLASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20796..22220)
                     /gene="lpdA"
                     /locus_tag="ON345_001676"
     CDS             complement(20796..22220)
                     /gene="lpdA"
                     /locus_tag="ON345_001676"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR3071756.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22545..>23975)
                     /gene="aceF"
                     /locus_tag="ON345_001677"
     CDS             complement(22545..>23975)
                     /gene="aceF"
                     /locus_tag="ON345_001677"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR3071757.1"
                     /translation="GTVKEIKVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAA
                     APAPAAGVKEVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAG
                     VVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKA
                     EGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRA
                     EAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFD
                     KTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRL
                     TLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGG
                     CFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHR
                     VIDGADGARFITIINNTLSDIRRLVM"
BASE COUNT         5842 a         6547 c         5862 g         5724 t
ORIGIN      
        1 tgctcaatgt caagttgact aatcccactt gttttaccgc tatgcttagt ggaacttctg
       61 tgactggttc tacggtaaaa atgggtgaat atagtgcaga acaaatcaga aacggtgcca
      121 caccggttcc ttttgatatt tcacttcaaa actgcgttcg tgtgactaat attgagacaa
      181 aattagtttc aacaaaggtt ggtactgaaa acgggcaact ccttggtaat actctcacag
      241 gtaatgacgc agcaaaagga gtcggtgtac tcatagaagg tttagcaact agtaaaaatc
      301 ctctaatgac attgaaacct aatgattcaa attctgttta taaagattac gacccgagag
      361 gcaaagacga tacaacagga ggagtttacc cggatcaaga tacaggtata acataccctc
      421 tccatttcca ggccacgcta caacaggatg gaactatacc aatagaagct ggtgagttta
      481 aagccaccag tactttccag gtaacctacc cttaataagt ccaccgcacc gccatcatct
      541 ggcggtgcgg taattgataa atcattgccc ccaccaatgt gactgataaa atgccaggca
      601 acacgacatc atttatcagg acacgttatg aaaccgacca ccattgcttc acttcagaaa
      661 tgtaagcagg ataaaaagcg tttcgcgacc atcaccgctt acgactacag cttcgcgaaa
      721 ctatttgctg atgaaggact taacgtcatg ctggtaggcg actcgctggg catgacggtt
      781 cagggtcacg actccaccct gccggtgacc gttgctgata tcgcctacca cactgccgcc
      841 gtacgtcgcg gcgcgccaaa ctgcctgctg ctggctgatc tgccgtttat ggcgtatgcc
      901 acgccggaac aagcctttga aaacgccgca acggttatgc gtgccggtgc caatatggtc
      961 aaaattgagg gcggtgagtg gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt
     1021 cctgtatgtg gtcacttagg tttaacccca cagtcagtga atattttcgg tggctacaaa
     1081 gttcaggggc gcggcgatga agcgggcgat caactgctca gcgatgcatt agccttagaa
     1141 gccgctgggg cacagctgct ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt
     1201 accgaagcac tggcgatccc ggttattggc attggcgcag gcaacgtcac tgacgggcag
     1261 atcctcgtga tgcacgacgc cttcggcatt accggcggtc acattcctaa attcgctaaa
     1321 aatttcctcg ccgaaacggg cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg
     1381 gagtccggcg tttatccggg cgaagaacac agtttccatt aaggagtcac gttgtgttaa
     1441 ttatcgaaac cctgccgctg ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc
     1501 gcgtggcgct ggtgcctact atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg
     1561 aagccaaagc ccgcgccgat gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg
     1621 accgcccgga agatctggca cgttatccac ggaccttgca ggaggactgc gagaagctaa
     1681 acaaacgtaa agtggattta gttttcgccc cttcggtaaa agagatctac ccgaacggta
     1741 ctgaaaccca tacttacgtt gacgttcccg ggctttcgac catgctggaa ggcgcgagcc
     1801 gtccggggca ttttcgcggc gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc
     1861 cggacatcgc ctgcttcggt gaaaaggact ttcagcaact ggcgctaatc cgcaaaatgg
     1921 ttgccgatat gggcttcgat attgaaattg tcggcgtccc gattatgcgc gccaaagatg
     1981 gccttgcgct cagttcccgt aatggttatc tgacggcgga acaacgcaaa attgcgcccg
     2041 gtctgtacaa agttttaagt tcaattgccg acaaattgca ggctggcgag cgggatcttg
     2101 atgaaattat tgccattgca gggcaagaac tgaatgaaaa aggcttccgc gccgatgata
     2161 ttcagattcg cgatgccgac acattgctgg aagtttctga aaccagcaaa cgggcagtaa
     2221 ttctggtagc cgcctggctt ggcgatgctc gcctgatcga caacaaaatg gtcgagctgg
     2281 cgtaatactt aactggcgct acggctgatg gcgccagtta ttaatttacc ccacgtcatc
     2341 tgtcataaca caggtaactt aaataaggtt aattacctta tcaatctcag caagaggtaa
     2401 atttgccatc tctgcaacgt cttcccgaga cattccttta cttagaagac gcagggcgaa
     2461 ttcttgcctt tcctcctgtc ttccctgctg aatccccttc tcaatccctt tctcttcaaa
     2521 ccactgcgcc agcgtcatca tagacttccc tcccgtttcc ctgtctctca gcacaccata
     2581 aaacaaatcc gcttgttcag tatgaccgcg ttgcagcata tagttttgca tggcaactaa
     2641 ctgacttccg ctagtgtacc cttcgtcaat cagcgtgacc agttgctcta gcaataacat
     2701 taagtcgcgc tggcgaatat gtttttgcag tagttcgaga atcgcaatcc gccgatgttg
     2761 catgatttcg tcatccggtg tgatggtgat atccaccagc gggaaaggac tgttatagac
     2821 gcgtcgcgcc agctccggcg agtaaaatat atcaaaccag cacattgaga gcggataagg
     2881 tgtggcctcg ctctgataaa acagtatcgg caccaccagc ggcagcttat cgtgatccgc
     2941 ctccagatgc cggtgcatgg cagcaataga ataacgcatc atgcgaaagg ccattttctt
     3001 atccggcttg ctttggtgtt caatcacaac atgcagataa ccgggattgc cctgcatttg
     3061 cacggaatag agcacgtccg tgctgtgtcc tttcaggctc tcttcaatga aactccccga
     3121 ctctaaatga agcgtgttga ggtcacaaag ttctcgtaat tccactggca aatgtatctc
     3181 cagaaagtcg cgagccgtct ccgcgtgcat taaaaattgt ttaaacactg catcatgcgg
     3241 tgtggtactc ggtgcatcca tcttccctgc tttccgtgtt gtcttgcgtg aaggcagtat
     3301 ccagtcaagg cgtgccatcg tcagccacct ctttagtgtt taccgagcag cgttcagaga
     3361 aatttcaatt ggttacagcc gttacatggc acgcttcggc acggtacgca aactgctgat
     3421 gtaaatgccg tcagatgagt agacactaaa caaaaatcgg gcaatactgc gtgagaattt
     3481 cctcaaagca ggcatcgcct gcttcgttaa cgacagggta gaaaggtaga agttatgatt
     3541 cgcacgatgc tgcagggcaa actccaccgc gtgaaagtga ctcatgcgga cctgcactat
     3601 gaaggttctt gcgccattga ccaggatttt cttgacgcag ccggtattct cgaaaacgaa
     3661 gccatcgata tctggaatgt caccaacggc aagcgtttct ccacttatgc catcgcggca
     3721 gaacgcggtt cgagaattat ttctgttaac ggtgcggcgg cccactgcgc cagcgtcggc
     3781 gatattgtca tcatcgccag cttcgttacc atgccagatg aagaagctcg cacctggcga
     3841 cccaatgtcg cctattttga aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg
     3901 gtacaggttg cttgattgtt atccctgcgg ctggttactc accagccgcg acatcgtctc
     3961 cagcgaatcc gttcttaaga tataaagtcg ttttagtaac aacggattat cccccggttt
     4021 tactttgcct ttcatggttg tcaccgccag gtgaaatccg gcatcgtttg ctgccttcac
     4081 ggctttgtca ttaaatccgc caaacggata cgaaagatac cagacatgcg gattaaattg
     4141 cgccagagcg cggcgtgaac gtgcaaaatc aaacagaata ttgtgctcac tacggctcag
     4201 taatatgggt cggcgatagc catccacccg atgcaaaaaa tgggtatgtg actgaaaatc
     4261 aaaaacatcg cgaatttcgt taagttcaga aacgctcata aattgcagcg attttgggtc
     4321 ccacttctgc gggtgacgtt tgatgtgcga ggtaacaata aacgccgtcg ccttcatgcc
     4381 atattgtttc aacacgggat acgcatagcg gctcaccgat ttgaggccat catcaaaggt
     4441 aatcaccacc gctcgcgcag ggagattgat cttattcttc acgtaacctt ccagctgcac
     4501 catgctcagt gtcgcgtatc ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg
     4561 tactgaggtg gtcgtcgaag tatggcgaaa acgggtgttt tcttcatcgc gcagaatatg
     4621 gtgataggtt agcaccggca ggccattatc gggttgggca tccagcgcgc tgatataggc
     4681 cagtcgatcg ccaatacgga tctgatacca ggtttgattt aacctgtctt tcagtttatg
     4741 caaaatcggg tagcgcaaat tgtccgccag taccccaaat ggcgcactgc ccgcactcgg
     4801 cgcgttatag accggcgtat ctttccaggt aattaagttc tgattactca gcggcttgtt
     4861 gaggtcaccc aaaccgtctt caactttttg tcgcccctga accggctcga gatgcccttt
     4921 atcgataaac cctttaccaa agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc
     4981 cacggcaatg atttgtccgg cccgaatatt ccccacggtc accatcttgt cgccaatttg
     5041 cgcccagacc gcagcatttt cgatggtttg catataacgg gcaggtaacg cggcactgac
     5101 acttgcggta aacagcatca gcaggagaat aacagcttgt ttgtacatga aaaaataacc
     5161 gagggtgaaa caacgctttt attgtagcaa aagcgctata agaatcgtgg gttaatactc
     5221 atacaaatta tgtttgagga cgaaaggcgg atttttttgt tgttggtgcc aaagactact
     5281 cacctccggc gcacccagtt cgacaatacg ctcgcgaaac tctgaactgg tcatggtttc
     5341 acggcaagcc atcatctgtt caattaacgg taacgtgaca ccagaaatca cttcgcaacg
     5401 ggagtgtttg tggcttaata atgaagccac gcggtacggt ggcgctccgg ctatatcggt
     5461 taagaagatg acaccctcac cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct
     5521 gagcatatta gagcttaacc cacgccagaa attcacggcc cggcactgaa gaagtgcccc
     5581 atgttttttc tccagcgcat ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac
     5641 ccaacctaac atcgccttcc tccttagccg aaaatcagtc taccctgacc attgttgata
     5701 aagggtgata gccgtcaaaa ctcctctccc ccaaatccag aggagaggaa atagccttag
     5761 ctacgcaaac cacgtccacg ttggatcagc gaccaacaga tcaaataaaa cgccacaata
     5821 aagaccacca gtacgccaaa ggtagtgacc agcggaacat cattgatacc gaggaagccg
     5881 tagcggaaac cactgatcat ataaacgatt gggttcaggt gcgacagccc ttgccagaac
     5941 ggcggcaaca aagtcagtga gtaaaagacc ccacccaaat acgtgagtgg cgttaacaca
     6001 aaggttggca ccaggctgat gtcatcgaac gttttggcaa acacaccgtt cagcaaaccc
     6061 gcaagggaga acaacaccgc cgtgagcacc agggttaagg caacgaatac ccacgaatgc
     6121 acctgaaacg gcacaaaaaa cagtgaaatt gccgtcacca gaatgccaac aaacagacca
     6181 cgcgccacgc caccgccgac atatccggca ataatgacgt gagtcggaac cggcgctacc
     6241 agcagctctt caatattacg ctggaacttg gcaccaaaaa atgatgacgc gacgttagcg
     6301 taggcgttgg tgatcaccga catcatgatc agccccggta cgatgaactg catatagctg
     6361 aagccatgca tatcgccaat acgcgaacca atcaggttac cgaagataat aaagtaaagg
     6421 gtcatggtga tgactggcgg caccagcgtc tgcacccaga tacgcataaa gcgatggatc
     6481 tctttcgccc agatgctttt tagcgccacc cagtaaagat gcatcatgcg cgatctcctt
     6541 gtttttcatt aaccagtgaa acaaacagct cttccagacg gttagctttg ttacgcatac
     6601 ttaatacctg aatgccctgc tcacttaact gcgtaaatac gctgttgatc ccctgctcac
     6661 gcagcacttc aacttccagc gtcgcggtat cgaccagtcg atactgatag ccatcgagct
     6721 tcggtaacgg acttttcggt gcgagatcga gaataaaggt ttccgatttc agcttcgcca
     6781 gcagcgcctt catcgaggta ttttccacga gctcaccgtg ttgaataatg ccgatattgc
     6841 ggcacagcat ttctgcttct tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt
     6901 cgtttaaatc cttcaaaaag ccccacattg agcggcgaag ttcaatatcc acgcctgcag
     6961 tcggctcgtc gagaatcagt agtttaggtt catgcattaa cgcacgggca atcattaaac
     7021 ggcgcttcat cccgccagat aacatacgtg cacgttcgtt gcgttttccc catagatcga
     7081 gttgtttaag atatttttcg ctgcggatgt acgcctcttt gcgctccaca ccgtagtacc
     7141 ctgcctgatt caccacaatt tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg
     7201 gcaccagtcc caactgacgt ttagcgttca cgacatcttt ctcgagatcg taaccaaata
     7261 cgctgacccg cccggaggtt ttatttacca gagagctgat aataccgata gtggtcgatt
     7321 tcccggcccc gttcggcccg agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta
     7381 ttccacgaag cgcctgaacg ccgcctggat aggttttttt aagctgttga agttccagtg
     7441 caatggtcat aaatttttac ttaccttacg ttcttacact ttatctgtgg tttaaatcgt
     7501 cccggagttg ccctatatta gccaaacgta attatttggt tacaggtcgt taacctccat
     7561 gaaagacata gatacactca tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga
     7621 ggatcccggg ttttttgaga aactggcaca agcgcaaaaa ccgcgctttc tatggattgg
     7681 atgttccgac agtcgcgttc ctgcagaacg tttaaccggt cttgagccgg gcgaactctt
     7741 tgttcaccgt aatgttgcta acctggtcat tcacaccgac ctgaactgcc tttccgtggt
     7801 tcagtatgca gtggatgtac tcgaagttga acacattatt atctgtggcc actacggttg
     7861 cggcggcgta caagccgcag ttgaaaaccc ggaactgggg cttatcaaca actggctgct
     7921 gcatatccgc gatatctggt tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg
     7981 ccgtctggat accttgtgtg aactgaacgt catggaacag gtttataacc tgggccactc
     8041 caccattatg caatcagcgt ggaaacgcgg gcagaaagtt accattcacg gctgggccta
     8101 cggcattcac gacggcttgc tgcgtgatct ggatgtcaca gcgaccaacc gcgaaaccct
     8161 tgagcaacgt taccgtcacg ggatttccaa cctcaagctg aaacacgcca accacaaata
     8221 aaaatgccat gccggatgca acacatccgg caacttcaca cttactcgtc cagcagaatc
     8281 actttgccga tatacggcag atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc
     8341 acaaactcat ccgggatcga gaaaccgata aattcaaccg ggacgttcac ttcacgacgg
     8401 gacggtttat ccagcagcgt acaaatcgcc aacgacttcg gttcgcgcag gcttaagatc
     8461 tcacgcactt tcgacagtgt attccccgag tcgatgatat cttcaacaat cagcacgtcc
     8521 ttgccacgga tatcttcatc cagatctttg aggattttca catcacgggt ggtggacatg
     8581 ccgctaccgt agctggaggc ggtcataaag tcgacttcat gagatacctg aacttcacgg
     8641 cacaggtccg ccataaacat aaatgagcca cgcagcagac caaccagcac catatcgctg
     8701 ccgctgtctt tgtaacgctc agtaatctga cgacccagtt cggcgatacg cgctttaatc
     8761 tccgcttcgg ggatcattac ttctacagta tgtttcatat ctctaaccat atgatttaaa
     8821 aacaaatcac ttcgcacggg caggttttgg caccgcaaca aagcgcaaga cacgcagtat
     8881 accagtaaaa ctattcattc gcggaatctg ttaataaagc tcgaattgtg atgacgatca
     8941 cacatgttaa aacctataat taattgctac taaaatatta atgaattgaa atggtgtctc
     9001 tttatggcaa ttaacaatac aggctcgcga cgattactcg tcacgctaac agcccttttt
     9061 gcagcgcttt gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc
     9121 tcctggtact accctatcgc tggtcttgtg atgctcggcg tcgcctggat gctgtggcgc
     9181 agtaaacgcg ccgcgctttg gctgtacgca gccctgctgc tcggcaccat gatttggggc
     9241 gtctgggaag ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc
     9301 ttcggcatct ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc
     9361 gcagttgccg cactggtggt ggcactgctg attagcggtg gtatcctgac ctgggccgga
     9421 tttaacgatc cgcaggaaat caacggcacc ttaagcgcca atgccacacc tgcggaagct
     9481 atctcacccg ttgccgatca ggactggcct gcctatggtc gcaatcagga aggtcaacgc
     9541 ttttcacctc tgaaacaaat taacgccgat aacgtccata atctgaaaga agcctgggtg
     9601 ttccgtactg gcgatgtgaa gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg
     9661 ccgattaaag tgggcgacac cctttacctg tgtaccgctc accagcgcct gtttgcgctc
     9721 gatgccgcca gcggcaaaga gaaatggcat tacgatcctg agctgaaaac taacgagtct
     9781 ttccagcacg taacctgccg tggcgtctct tatcatgaag ccaaagcaga aacggcttcg
     9841 ccggaagtga tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgactg
     9901 attgcgatta acgctgaaaa cggcaaactg tgcgaaacct tcgccaataa aggcgtgctc
     9961 aatctgcaaa gcaatatgcc agacaccaaa ccgggtctgt atgaaccgac ttcgccaccg
    10021 attatcaccg ataaaaccat cgtgatggcc ggttcagtta ccgataactt ctcaacccgc
    10081 gaaacgtctg gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt
    10141 gatcccggcg cgaaagatcc gaacgcaatc ccgtctgacg aacacacctt tacctttaac
    10201 tcgccaaact cctgggcacc agcggcctat gacgcgaagc tggatctggt ctatctgccg
    10261 atgggcgtga ccacgccgga tatctggggc ggtaaccgca caccggaaca ggaacgttat
    10321 gccagctcga ttctggcgct gaatgccact accgggaaac tggcgtggag ctaccagacc
    10381 gttcaccacg acctgtggga catggatctt ccggcacagc cgacgctggc ggacatcacc
    10441 gttaatggtc agaaagtgcc agttatttac gctccggcga aaaccggcaa catttttgtg
    10501 ctcgatcgtc gcaatggcga actggtggtt ccggccccgg aaaaaccggt gccgcaaggt
    10561 gctgcgaaag gcgattacgt tactccgacc cagccgttct ctgaactgag cttccgtccg
    10621 aagaaagatt tgagcggtgc ggatatgtgg ggagccacca tgtttgacca actggtgtgc
    10681 cgcgtgatgt tccaccagat gcgctatgaa ggcattttca cgccgccatc tgaacagggc
    10741 acgctggtct tcccgggtaa cctgggaatg tttgaatggg gcgggatttc cgttgatcca
    10801 aatcgtgaag tggcaattgc caacccaatg gcactgccgt ttgtttcgaa actgatccca
    10861 cgcggtccgg gtaacccgat ggagcagccg aaagatgcca aaggcacggg tacggaatcc
    10921 ggcattcagc cacagtacgg tgtaccgtat ggtgtcacgc tcaacccgtt cctctcacca
    10981 tttggtctgc catgtaaaca gccagcatgg ggttatatct cggcgctgga tctgaaaact
    11041 aatgaagtgg tgtggaagaa acgtattggt acgccgcagg acagtatgcc gttcccgatg
    11101 ccggttccgg tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat ctccacggcg
    11161 ggtaacgtgc tgtttatcgc cgctacggca gataactacc tgcgcgctta caacatgagc
    11221 aacggtgaaa aactgtggca gggtcgttta ccggcgggtg gtcaggcaac accgatgacc
    11281 tatgaagtga atggcaagca gtacgtggtg atctccgcag gcggtcacgg ttcatttggt
    11341 acgaagatgg gcgactatat tgtggcttat gcgctgccgg atgatgtgaa gtaagacttg
    11401 ctcagattgc tgacaacgtg cgcgttgttc atgccggatg cggcgtgaac gccttatcca
    11461 gcctacaaaa ttgtgcaaaa tcaatggatt gcacaggtag cgtaggcctg ataagcgtag
    11521 cgcatcaggc agatttgcgt ttgtcatcag tttaatgccc ggagagatcc gggcatattt
    11581 ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag
    11641 cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag
    11701 cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc
    11761 tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg
    11821 cagcatcatg tcgcccacac cagagataac ccaacgttcg tactgccctt tcgccgccgc
    11881 aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc
    11941 gaacttcccg ccatggttca tatgattcat attgccgtgc cccatatggc ccatcatctg
    12001 gctatgatcc atcccggcca tcgcctgatc gccatatttc tccatcagca tctgcatccc
    12061 catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag
    12121 cgaaggtaac gcaggcaggc tacttaatgt gtctggcaaa gcaccggagg cactaatagc
    12181 aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg ccatccccat
    12241 ctggctgacc ggtagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac
    12301 ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag
    12361 accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga
    12421 gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgtgggg cagcgtgttg
    12481 tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat
    12541 cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct gaacaatcac
    12601 cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc
    12661 aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc catgttgatg
    12721 tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc
    12781 acctggcgga ataattccct gggggccgcc gtccacttca cccggtactt ccagcccgtg
    12841 ccagtgcaac gtcgtctctt ccgtcagttg gttatagata tcaaccgtta ccgctttgcc
    12901 gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcagt
    12961 tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc
    13021 ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga ctgcgcggct
    13081 ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga aatcacgacg
    13141 ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc
    13201 cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc
    13261 ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg
    13321 tttttcagaa cagtgttatt cggcagcctg atggccgttt gcgcaaacag ttacgcgctc
    13381 agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt tctgaagaac
    13441 gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt ctttttcgcc
    13501 cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc tctcggtgaa
    13561 gacagctacc gcgatctcag cggcattggc attcctgtcg ctaaaaaatg caaagccctg
    13621 gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga
    13681 aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt tttttacggg
    13741 tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac attggcatga aacgctacac
    13801 gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac
    13861 caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct ggatggcgta
    13921 gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca tgttccgcta
    13981 ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg
    14041 cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt
    14101 gtcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta cgacgatccg
    14161 cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag ccagaccttt
    14221 gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct gttcacctca
    14281 gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac
    14341 ggcgtctgct ttttacagca ggaagaagcc atcgacagcc atcgcaaact cagccattac
    14401 ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg tatcatgact
    14461 tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg
    14521 cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca tacggcagct
    14581 tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta aggagaagat
    14641 aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt
    14701 tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta tattgcttat
    14761 atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt
    14821 atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg tgccagcgtc
    14881 actattctgg tgagtgaaga gccggttgac ccgaaactca tcgacaaaac agaacacccc
    14941 ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg cgtacatacc
    15001 tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct
    15061 acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc
    15121 gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa cggtatgaag
    15181 cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg
    15241 ctgtatgaca tggtggatgt aaacgtctat caggaaaata tcttccatac caagatgttg
    15301 cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac
    15361 agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg
    15421 cgcaatatgc cagctgttta acgtctcggg cggagctccc aggctccgcc agatttattt
    15481 acttctgctg cacgaaatta cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc
    15541 atagcgacag gctttcttcg tcgtagtagt tcatcccctc ttccatttca tcgccagcga
    15601 attccagttg attggcgcga accatcacct cttcaccgtc catccacagg gtgtattcat
    15661 gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct tgttccactt
    15721 catcaagcaa ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat
    15781 gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca
    15841 tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt gatgtaatta
    15901 tcgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa aaaatgcccg
    15961 ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca
    16021 ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt
    16081 tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact
    16141 cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca
    16201 ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag gtggaaacca
    16261 ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag cccgggatct
    16321 cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc aactgtgcgg
    16381 cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct ttgtgcgcat
    16441 ccagcagttt acccgcagca cggaagtggc cgatgttagt catgcaagaa ccgataaaca
    16501 cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc gggtcgttcg
    16561 gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact gccgcgtatt
    16621 cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa
    16681 tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac ttcagcagga
    16741 cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag
    16801 cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact ttcagatccg
    16861 gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa
    16921 cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga tcgcgcaggg
    16981 tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg
    17041 gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg aaagagatac
    17101 cgatcgggaa acgggtatgg gagtcacctc cggtaccgac ggtatccggc agcagcatac
    17161 ggttcagcca ggagtggatt acgccgtcac ccggacacag cgacacaccg ccacggttca
    17221 taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg
    17281 tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca
    17341 gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt
    17401 cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg
    17461 ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt
    17521 gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa
    17581 tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca
    17641 gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt
    17701 cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg tcttccatcg
    17761 tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accacagccg cgtttgttcg
    17821 gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg
    17881 atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag
    17941 cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg
    18001 cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac gcatccggtg
    18061 ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta acggtcagtt
    18121 tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct
    18181 gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag ttatcgaaca
    18241 tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca tcatccagcg
    18301 cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt tcgatggctt
    18361 tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc aggaagcctg
    18421 ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat agatctaaca
    18481 ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg gccatttggt
    18541 ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt
    18601 attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct
    18661 tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat
    18721 agcaggattt tgacgatgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga
    18781 atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt
    18841 gataatcacc tttctcaacg aaccccaaga ttagtcaaaa tttaaactac cgcctcttta
    18901 tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat
    18961 ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc
    19021 cgggctttgt gccgcctcta ccgggctata tataaaaagc cgcacagtgg aagcgcctgt
    19081 ggaaccgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc ttcctgcaac
    19141 ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca ctgcacaaat
    19201 cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac aagtgttaat
    19261 ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg ataatccact
    19321 taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa
    19381 atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca
    19441 aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt
    19501 gcggcaactg gcgcaacaaa ccggttcgct gaagtcatca accgaacaaa aagttattac
    19561 cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa
    19621 taccgtttta actgccagcc ccgtcattac agagcctgca acaaccgtca tttccattga
    19681 gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa
    19741 ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttat
    19801 tgacagcttt gtgcgcggat tcggctatag catgggtgtt gctaccacgt acgcactatt
    19861 cagcagcatc gactgggacg acgacgatca tgaccatcat catcatgacg atgatgatta
    19921 tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg gcgacaacat
    19981 caatatcgac gttaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat
    20041 ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc
    20101 aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc
    20161 aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc
    20221 ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga
    20281 acactatggg agctatgacg acttccgcga cttcagccgt cgccaacccc tgacccagca
    20341 acaaaaggac gccgctcgtc agcgttatca gtcagcttct cctgagcagc gccaggcagt
    20401 tcgcgagaga atgcagacta acccgcagat ccagcagcga agagaggcag cgcgtgagcg
    20461 tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac agcagcgccc
    20521 actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caattggcat cacctgaaca
    20581 acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa acgacagtaa
    20641 ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc tctctgagcg
    20701 cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt ggttagccgc
    20761 ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc tttcgggttc
    20821 ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga ctcgtgcaga
    20881 gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat ttcgattgcc
    20941 aggccgattt cacccagcag ctcgccgccg ttggtaccga caatcgcacc accgatcaca
    21001 cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc ggaagcgata
    21061 gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc tttctctttc
    21121 gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc gatagacggg
    21181 ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc aacgtgacct
    21241 tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat agcaaagatg
    21301 tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc gtccacttcc
    21361 acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat cgctaccagc
    21421 acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt cacataaata
    21481 ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt gaatttcttg
    21541 ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat aacctggtcg
    21601 aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc catttccaga
    21661 ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag ttccagcgcg
    21721 tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg gatcgggcga
    21781 gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt ctcaccttca
    21841 acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt gactttgcgg
    21901 cctttcgcca taccagccag accaccggtc agctgattga tcactttctc tttccaggta
    21961 cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc agccagcgct
    22021 ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat acagccgacg
    22081 ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc cagacctaaa
    22141 tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac cacgacctga
    22201 gttttgattt cagtactcat catgacctct atatatttat ctccggcggt catacccgtc
    22261 gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt acttatgtaa
    22321 gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata gggtatgtga
    22381 agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa aattgttaac
    22441 aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc aatacattca
    22501 tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat caccagacgg
    22561 cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gagcaccatc agcaccgtcg
    22621 atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac gaactcttta
    22681 ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac ttccggcgcg
    22741 ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat ggtgaagcaa
    22801 ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga aatagtcatc
    22861 agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac cggaacaacc
    22921 agaccgttcg gggtatccac cgccacaccg atgttgatgt acttcttcag ggtcagacgc
    22981 tgaccgtcct ccgacagcga actgttgaag cgaggcatct gctcaagagc tgcagcaacg
    23041 gctttcatga tgaagacaac cggggtgatc ttcacatcca gcttacgttt cgccgcttct
    23101 tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc gaagtgagta
    23161 acatgcggga tcatcaccca gttacggctc aggttcgcac cagaaatttt ctggatacgg
    23221 cccagttcca cttcttcgat ttcaccaaac ttgctgaagt ccaccttcgg ccacggcagc
    23281 atgcctggga taccgccgcc agtcgccgcc ggagccgctt ctgcacgttt gatagcttct
    23341 ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacggccagt gcccttcact
    23401 ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt cgcgtgaacg
    23461 taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg agctgctgcc
    23521 ggagcttcag cttttgctgc cggagccggc gctgccgctt cctgtttcgc cggagccgcc
    23581 gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt cactttatcg
    23641 ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccggaac ttccatagaa
    23701 gctttgtcgc cttctacggt aatcagtgac tgttcagcgg caactttgtc gcccactttc
    23761 accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt aacttctttc
    23821 acgccagccg ctggtgcagg ggccgctgcc ggagccgctt cctgtttagc cgccggagct
    23881 gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt agacactttg
    23941 tcacccacgt tcactttgat ctctttcacg gtgcc
//
LOCUS       DAJSKJ010000050        23930 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000050 DAJSKJ010000000
VERSION     DAJSKJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.050"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ON345_001678"
     CDS             complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ON345_001678"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR3071758.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRAT"
     gene            complement(3868..4086)
                     /gene="ybdZ"
                     /locus_tag="ON345_001679"
     CDS             complement(3868..4086)
                     /gene="ybdZ"
                     /locus_tag="ON345_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR3071759.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4089..5291)
                     /gene="fes"
                     /locus_tag="ON345_001680"
     CDS             complement(4089..5291)
                     /gene="fes"
                     /locus_tag="ON345_001680"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR3071760.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNANFWLAVQQELLPLVKAIAPFSDRGDRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5534..7774
                     /gene="fepA"
                     /locus_tag="ON345_001681"
     CDS             5534..7774
                     /gene="fepA"
                     /locus_tag="ON345_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR3071761.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7949..8569
                     /gene="entD"
                     /locus_tag="ON345_001682"
     CDS             7949..8569
                     /gene="entD"
                     /locus_tag="ON345_001682"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR3071762.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8691..8843)
                     /gene="hokE"
                     /locus_tag="ON345_001683"
     CDS             complement(8691..8843)
                     /gene="hokE"
                     /locus_tag="ON345_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR3071763.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9194..9346)
                     /gene="hokE"
                     /locus_tag="ON345_001684"
     CDS             complement(9194..9346)
                     /gene="hokE"
                     /locus_tag="ON345_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR3071764.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9800..10918
                     /locus_tag="ON345_001685"
     CDS             9800..10918
                     /locus_tag="ON345_001685"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR3071765.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQQQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVAGGGSLIGLVKKHCEIWAGD"
     gene            10984..11232
                     /locus_tag="ON345_001686"
     CDS             10984..11232
                     /locus_tag="ON345_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR3071766.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11312..11665
                     /locus_tag="ON345_001687"
     CDS             11312..11665
                     /locus_tag="ON345_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR3071767.1"
                     /translation="MHETAKHLALELPFVELCWPFGPEFDVFKIGGKIFMLSSELRGV
                     PFINLKSDPQKSLLNQLIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLINDSWNLV
                     VDGLVKRDQKRVRPG"
     gene            11759..12412
                     /gene="nfsB"
                     /locus_tag="ON345_001688"
     CDS             11759..12412
                     /gene="nfsB"
                     /locus_tag="ON345_001688"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR3071768.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12520..13767
                     /gene="ybdG"
                     /locus_tag="ON345_001689"
     CDS             12520..13767
                     /gene="ybdG"
                     /locus_tag="ON345_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR3071769.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13835..15211)
                     /gene="pheP"
                     /locus_tag="ON345_001690"
     CDS             complement(13835..15211)
                     /gene="pheP"
                     /locus_tag="ON345_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR3071770.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15313..18456)
                     /gene="cusA"
                     /locus_tag="ON345_001691"
     CDS             complement(15313..18456)
                     /gene="cusA"
                     /locus_tag="ON345_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR3071771.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSTVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18468..19691)
                     /gene="cusB"
                     /locus_tag="ON345_001692"
     CDS             complement(18468..19691)
                     /gene="cusB"
                     /locus_tag="ON345_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR3071772.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19707..20039)
                     /gene="cusF"
                     /locus_tag="ON345_001693"
     CDS             complement(19707..20039)
                     /gene="cusF"
                     /locus_tag="ON345_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR3071773.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20063..21445)
                     /gene="cusC"
                     /locus_tag="ON345_001694"
     CDS             complement(20063..21445)
                     /gene="cusC"
                     /locus_tag="ON345_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR3071774.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21602..22285
                     /gene="cusR"
                     /locus_tag="ON345_001695"
     CDS             21602..22285
                     /gene="cusR"
                     /locus_tag="ON345_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR3071775.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22275..23723
                     /gene="cusS"
                     /locus_tag="ON345_001696"
     CDS             22275..23723
                     /gene="cusS"
                     /locus_tag="ON345_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR3071776.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5782 a         6522 c         6052 g         5574 t
ORIGIN      
        1 gcgttgcgcg aataatcggc ccaattttta caaacgcccc tggagagata atatccacat
       61 gcgcacaatc ctgacgatag atatccagct ccgcaatcca cggcgaccag gcgcgttcag
      121 gactcatacc ttcctgaagc gtgcgttcag caacaaacaa cgtcgcttta ccgtcaaacg
      181 gtacgctatg agcagtcgtc agcaggcgca cagcatcagc gtagttgcct tctatggtgg
      241 taaacaactc cgttgaagta cttccctgct gtgccgccag gaaggcctcg cgctcgcggt
      301 taatctccgc cagcacttcc gggtccagac cattagcttc tttttcctgc cagttttgcg
      361 tttctggcgg ccaggtatcc agcaagccaa gaaatgccac ctgttcgcca cgagcacgca
      421 gtcgcgccgc aataccctgc gccagcgtac cgccaaggga ataccccagc aggtaataag
      481 ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc ttcgcagact tcatccaggt
      541 ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg aatgccaata atcgaccatt
      601 gtggatcgag ataacgcgag agcacgctaa actgccaggc aaaaccggac gccggatgga
      661 aacaaaacag cgtcgggcca ttaccttcac gcaacggcag aatggtttcg aatcccatgc
      721 gccgggagct gtcctcttca ccatcaataa tcgttgccag tttggcgacg gttgacgcga
      781 ccatcacctg ccccggcgtc acctgacgag caaactgtcg acttaactgc gctgccagtt
      841 tcattgccag tagcgaatga ccgcccagcg caaagaaatc agcatcggca tcctgcacgt
      901 cacaacccag caacgacgcg aatgccgcgg cgataatcgt ttcactgccc gctttcggtg
      961 cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa ggctttgcga tccagcttgc
     1021 cgttggcgct aagcggcaac tgcggaagtt gcagcagaac gaccggcacc atatgcggcg
     1081 gcaatgtttc gcgaagctgt gcctgtaatg cgctggtatc caacggcagt cccgattgcg
     1141 acaccagata gcccaccaac tgacgcgcat caccaccggt ggccgccgcc tggttaatca
     1201 cacaggcgtg ggtaactgcc tgttcgacat ccggcagcgc ctgcatcacg cgatcgattt
     1261 cgcccagttc gatacgctgc ccgcgaattt ttagctgatc gtcactgcgc ccgaggtact
     1321 ccaccgcgcc gttatccagc cagcgggcga cgtctccggt acgatacatc cgttcacctg
     1381 gggcaaaagg atcggcaata aaacggctgg cggtcagatc gggtcgtcca agatacccct
     1441 gcgccagttg gatgccggtg agatagagat cacccgccac acccggcggc accggatgca
     1501 tcatcgcatc aagaatacgc agacccgtat tccataccgg ataaccaatc ggcacactgc
     1561 tgccgcgcac ctgtgccagt tcctcgccaa aagccggata ccagctgaca tctaccgccg
     1621 cttccgtcgg gccatacagg ttatgcaacg gcgcgccggt taactgttgc cattcgcggc
     1681 ataaatcagc cggtaaggcc tcaccactac agaaaacctg tttcaacgtc gcgcaattct
     1741 ggcgagcggt ttgcggcgtc agcgatgcaa caaatgccgc cagcatcgac ggcacaaagt
     1801 gcgtggtcgt tacgccatat tcggcaaaga attgctgcat agcgagcggg tcacggtgcg
     1861 cttccggttc agccatcacc agttttgccc ctgcgataaa cggccagaaa aactcccaca
     1921 ccgagacatc aaaactgcac ggcgtttttt gggcaacgac atcttcgcct gtaagcgggt
     1981 agtgattttg catccataac aggcggttaa cgatagccgt ctgcccgacc attacccctt
     2041 tcggcctgcc ggtggagcca gaggtaaaga tgatataagc cgtgtggtgc ggttgtgaaa
     2101 gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt atagcaaagg cttgttaaat
     2161 tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt aattaacagc gacggacgcg
     2221 catcttccag catcattttc aggcgatcgt ccggatagcc ggtatccagc ggtagccagg
     2281 ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa aaagaccgag cgcggtagtg
     2341 ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg cagcagattc gccagcgcca
     2401 ccacctgctc gcgcatttcc cgatagctga acaggtaacg cgcatctgcc agcgccggag
     2461 catccggtgt ttttgccgct tgttctgcca ccagcgcgct cagcgtggtt tctggaatct
     2521 caacctgagt ggcgttgatc tgcgccagct gcgcatactc acctggcagc ataatatcga
     2581 catcgccgca caacagcgcc ggatctgcgg cgaactgggc aatcagcatt ttcaggcgtt
     2641 cagcatgctg gattaacgtt ggctcatcgt aacgctgttt attggcgagg atctcaatac
     2701 tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag gtcattaacc ggaccggttg
     2761 ccagggtatg ggtttgcgcc tgaacaccag gaatatccag ttggtaatca aataccttaa
     2821 tattgagtac cggaccaaac agcggttcat cacctgccgc tcgcccgctg tcacggacaa
     2881 tttgttcggc atcgtaacgt tgatggcgac gcattttttt cagttgagcc gccagtcggg
     2941 ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat acccaacggt aaaacgttga
     3001 gcacgggtcc gctagccgtc agcgccgccg agcccagtcg acgcataaag ataaatccgg
     3061 cggcatagtc catgcgattg cacaatcgcc ccagccacaa ggctgccagc gcaagggcta
     3121 aatcggtacg ctgcacacct gaaagttgcg tagccagctg gcggaattcc ccgtcggtaa
     3181 attccagttt caggcgcaga atatctgccg aagcgctgcg ccccgctaaa ggtgccggag
     3241 aaagtgacgc gggcggcggc agttgacgac gctgttctgc ccagaatgcc gcatcacgct
     3301 gccaggcttc gctttcgcgg tattgctggt actcttccac tacatcagcg aaaggcgtaa
     3361 acggcgaagc aggcgttggt tcgccacgca gccatgtgca gtaaatattg gcgatctggc
     3421 gagtaatggc cgggaaactg aagccatcga ccagcaaatg gtgataacgc tgataccagt
     3481 accagcggtt atccgcaaca tgtatcagct gatgaaagac cagtggttta ccgctatcga
     3541 cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg cgcagtaccg tgcggatcaa
     3601 tattggttcg caggtcgata atttctggca gttcgaatat cagcgcatca tcgacccact
     3661 gccagacttc gccgttatct tccgtaaaac gcatccgcaa cgtatctgct tgcgctagtc
     3721 cggcaaccac cgcgcgggcc agtaatggcg catcaacctc tccggttaac tcaacgtaat
     3781 gcgctacgct ccaggcggag ggtaaatccg acagtttttc tgccatccag atgccgggct
     3841 gtgcggcgac caaaggtaaa tgctggctca ttgcgcctcc tgcaactggg tgaaattcgc
     3901 cggtgtcaga gtacgccagt gggcttcaag ccactgctgg caggactctt gtgactgcgg
     3961 ctgacacaca atgtcccagc ctgccggtaa ggcgcattgt tgcggccaca gactgaattg
     4021 cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga tcatcgaagg gattactgaa
     4081 tgccatattc aactcctgtc atggaaaagt ggttgccaga ggtcgattag cccctgcatc
     4141 aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa cctgacgcca gaaaatggat
     4201 tcttttatgg ggtgtaattg cgcatacagc gcctgattgg cccgcataat catcggctcg
     4261 cgaatacccg cttccagcac aatgcgcaga ccttcggcgc taacttcacc agctttaagc
     4321 ttttcaagta acacgccctc ttgctgcccg ccccgatgcg gccaccagta cgatcctgac
     4381 tggcttaata cacaaccaaa acgttcaggc cagtgcagtc cggcatacag cgcggaaagt
     4441 ccaccaaaac tctgcccggc aaccacggtg cgatcgccgc gatcgctaaa aggggcaata
     4501 gctttcacca ggggtaataa ctcttgctgt actgcgagcc agaaattcgc attacacggc
     4561 agttcgtgag cgcggtgcgt ggtgtcgata gcgtcgatca acacatacac ggcgggagga
     4621 agttgctgac gatgggtcag cgaagtcagc acaggccaga cgggcatact ttgcgcccaa
     4681 aattcgccat cgagcaaaac tgccagcgga cgttcttctg ctgttacatc gccggtggta
     4741 aaaatccata cacgacgtga atttttcaac cgttcacttt tccagataat ttctttggct
     4801 gctatttctg gcgcttgcgg acaatcccat cccggttgca gaggcgcttg cggcatttcg
     4861 agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt gtggattcag cggatcggct
     4921 attgcctggg gtaatagttt tcgccagcct tcacgtaatt cgaggcgatc gggggatggt
     4981 gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt agctgccgcg ccagttggca
     5041 ttgagttgtg tcgtccactg ccagacatca gtacctgcaa ttcgctgcat cgactggggc
     5101 tggctgttct gatgatgatc ggttacacca gtgatgtaga tccatacgcg ctttatcgtc
     5161 gagtattctt cagaaccttg gggatcacgc caccagaaag tgacctcaaa catttcgtca
     5221 ttcagacgct gccattccgg gccatgtttc gactgccacc agctctcact tcctactttt
     5281 aacgccgtca caccataacc ccatgtttac tgtgcaattt ttcattgatt gcagaaatat
     5341 attgataata ttattgataa ctatttgcat ttgcaatagc gtaatggcgc gccgtgggaa
     5401 gcgcggacat ttattaacca actgcactgc gtgtctttca ggatcaaagg ttttcgcggt
     5461 agcgggatgc gtcgtgttga ggacgaccat gcccgacagt tgcaattcgt ggcaaaaatg
     5521 caggaataaa acaatgaaca agaagattca ttccctggcc ttgttggtca atctggggat
     5581 ttatggggta gcgcaggcac aagagccgac cgatactcct gtttcacatg acgataccat
     5641 tgtcgttacc gccgccgagc agaacttaca ggcgcctggc gtttcgacca tcaccgcaga
     5701 tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag atcatccgta ccatgccagg
     5761 cgttaacctg accggtaact ccaccagtgg tcagcgtggg aataaccgac agattgatat
     5821 tcgcggtatg ggtccggaaa acacgctgat tttgattgac ggcaagccgg taagcagccg
     5881 taactcggtg cgtcagggct ggcgtggcga gcgcgatacc cgtggtgata cctcctgggt
     5941 gccacctgaa atgattgaac gtattgaagt tctgcgtggt ccggcagctg cgcgttatgg
     6001 caacggcgcg gcgggcggcg tggttaacat cattaccaaa aaaggcagcg gtgagtggca
     6061 cggctcctgg gacgcttatt tcaatgcacc agaacataaa gaggaaggtg ccactaaacg
     6121 caccaacttc agcctgaccg gtccgctggg cgacgaattc agcttccgct tgtatggcaa
     6181 cctcgacaaa acccaggctg acgcgtggga tatcaaccag ggccatcagt ccgcgcgtgc
     6241 cggaacgtat gccacgacgt taccagccgg gcgcgaaggg gtgatcaaca aagatattaa
     6301 tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg gaactggaag caggttacag
     6361 ccgccagggc aacctgtatg cgggcgacac ccagaacacc aactccgatt cctatacccg
     6421 ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag aactactcgc tgacctggaa
     6481 cggcggctgg gataacggcg tgaccaccag caactgggtg cagtatgaac acacccgtaa
     6541 ctcgcgtatt ccggaaggtc tggcgggcgg taccgaaggg aaatttaacg aaaaagcgac
     6601 acaggatttc gtcgatatcg atcttgatga cgtgatgctg cacagcgaag ttaacctgcc
     6661 gattgatttc ctcgttaacc aaacgctgac gctgggtacg gagtggaatc agcaacggat
     6721 gaaggacttg agttccaata cccaggcgct gaccggggcg aacaccggcg gcgctattga
     6781 tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca gaaattttct cgctgtttgc
     6841 cgaaaacaac atggagctga ctgacagcac catcgtaacg ccggggctgc gtttcgatca
     6901 tcacagtatt gtcggcaata actggagccc ggcgctgaac atatcgcaag gtttaggcga
     6961 tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa gcaccgagcc tgtaccagac
     7021 taacccgaac tacattctct acagtaaagg tcagggctgc tatgccagcg cgggcggctg
     7081 ctatctgcaa ggtaacgatg acctgaaagc agaaaccagc atcaacaaag agattggtct
     7141 ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg ttccgtaacg attatcgcaa
     7201 taagattgaa gcgggctatg tggctgtagg gcaaaacgca gtcggcaccg atctctatca
     7261 gtgggataac gtgccgaaag cagtggttga aggtctggaa ggatcgttaa acgtaccagt
     7321 tagcgaaacg gtgatgtgga ccaataacat cacttatatg ctgaagagtg aaaacaaaac
     7381 cacgggcgac cgtttgtcga tcatcccgga gtacacgttg aactcaacgc tgagctggca
     7441 ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg tacggtaagc agcagccgaa
     7501 aaaatacaac tacaaaggtc agccagcggt tggcccggaa accaaagaaa tcagtcctta
     7561 cagcattgtg ggtctgagcg cgacctggga tgtgacgaag aatgtcagtc tgaccggcgg
     7621 cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt aatgcccaga ccacgggcga
     7681 tctggcaggg gccaactata tcgcgggtgc cggggcgtat acctataacg agccgggacg
     7741 tacgtggtac atgagcgtaa acactcactt ctgatgctaa cgtcagattg ttgacaaagt
     7801 gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa aatcttgcca
     7861 attcaatata ttgcaggacc atgtaggcct gataagcgta gcgcatcagg cagtttagcg
     7921 tttgtcatca gtctctaata tggtcgacat gaaaactacg catacctccc tcccctttgc
     7981 cggacatacg ctgcattttg ttgagttcga tccggcgaat ttttgtgagc aggatttact
     8041 ctggctgccg cactacgcac aattgcaaca cgctggacgt aaacgtaaaa cagagcattt
     8101 agccggacgg atcgctgctg tttatgcctt acgggaatat ggctataaat gtgtgcccgc
     8161 aatcggcgag ctacgccaac ctgtctggcc tgcggaggta tacggcagta ttagccactg
     8221 tgggactacg gcattagccg tggtatctcg tcaaccgatt ggcattgata tagaagaaat
     8281 tttttctgta caaaccgcaa gagaattgac agacaacatt attacaccag cagaacacga
     8341 gcgactcgcg gactgtggtt tagccttttc tctggcgctg acactggcat tttccgccaa
     8401 agagagcgca tttaaggcat cggagagaca aactgaagca ggttttctgg attatcagat
     8461 aattagctgg aataaacagc aggtcatcat tcatcgtgag aatgagatgt ttgctgtgca
     8521 ctggcagata aaagaaaaaa tagtcataac tctgtgccaa cacgattaat tgacaacatc
     8581 tggtacgatt cgcccgcagc catcactgac tgcgggcgaa agtgtaaagc aggtgcctta
     8641 ccactctgac ctgacaaccg gatatgcagg gattgctccc cgcacaacgg ctacttcttc
     8701 ggttcgtaag cgagaacagc cttaaactca atattacgtt cctttacggt gaactcacac
     8761 agcgagtctc cgaccagaag cgtaaatccc agtaccgtca aacacagcac tatgactgcc
     8821 acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc
     8881 cgagttgttg aggctcggag tggcagcccc aggattgata gaaatatctc ctggggcttc
     8941 atctttccag acctcagagt taacctgaaa ccggaaagct tcaggcaccc gccgttatct
     9001 tacccttcct caacaaaatg gctgtttatt tatacagtga atcctgtttc ggacttctgt
     9061 cggcaaaact catcattgct cttaaaacga aatcgcccgc agccatcact gaccgcgggc
     9121 gaaagtgtaa agcaggtgcc ttaccatcct gacctgacaa ccggatatgc ggggattact
     9181 ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccttaaac tcaatattac
     9241 gttcctttac ggtgaactca cacagcgaat ccccgaccag aagcgtaaat cccagcaccg
     9301 ttaaacacag cactatgact gccgcaaggg catatttcgt cagcatgttt atattgcctc
     9361 catgcgaaga agggactacc atccaaattg ttcaggtttg ggtaggtagc cccagggttg
     9421 atagaaatat cgtcctgggg cttcatcttt ccagacctca gagtaaacct gaaaccggaa
     9481 agcttcaggc acccgcagtt atcttaccct tgcttagcaa aatagctgtt tatttataca
     9541 gtaagtcctg tttcggactt ctcactcccc atttgatcaa ctgaagctga acccgttttt
     9601 ttctgcatgc ctcaatcgtt aaaattcttc gccaccagtc agcactgcca aaacttgtca
     9661 cattgcaaaa gtagccaaat tgcttctgtc ataatcagtt caccatcacc aaaaaaaaga
     9721 aacattccag aacttccgca tctcgcccag ccgctcccac ttctatactg aatagaaaac
     9781 gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac cttttacact
     9841 cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa gccaggactc
     9901 ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa agcacgatat
     9961 caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc aggctgccgg
    10021 gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc atctggaaat
    10081 ttgcggcggt ggcacacacc cgtttcagaa atggcagcaa caggaggtat gcgataacga
    10141 gcgctatcaa cgaacgctgg aaaactttgg ttatctcatt caacaggcga ccgtttttgg
    10201 tcagcatgtt catgttggct gcgccagtgg cgatgacgcc atttatttgc tgcacggcct
    10261 gtcacggttt gtgccgcact tcatcgccct ttccgccgcg tcgccatata tgcaaggaac
    10321 ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcctg ataatggccc
    10381 gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtc tgagttacac
    10441 cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc ctcattttgg
    10501 cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg taaatatggc
    10561 gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca aacatcagga
    10621 gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg ggcttgaagg
    10681 cgtcatcacc gatccgcaca ctggagatcg tcgaccgcta acggaagata ccttgcgatt
    10741 gctggaaaaa atcgcccctt cggcacataa aattggtgca tcgagcgcga ttgaggccct
    10801 gcatcgccag gtcgtcagcg gtctgaatga agcgcagcta atgcgcgatt tcgtcgccgg
    10861 tggcggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg gtgactaagt
    10921 agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac atttaacaat
    10981 gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct gatggctttc
    11041 ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca aaaactggtg
    11101 accatgttcc atctgatgga tcttagccag ctttacactt tattgttttg tctgtggttt
    11161 ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg ctggttctcg
    11221 ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt attctccttc
    11281 gcctaaagga acgtttaagg ataagcaatc actgcacgaa acggcgaaac acctggccct
    11341 tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg tttttaaaat
    11401 tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta tcaatctgaa
    11461 gtccgatcca caaaaatccc tgttaaatca gctgatatac ccgagcatta agccagggta
    11521 tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct ccgaagcgtt
    11581 acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg ttaaacgcga
    11641 tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc tcgcttacca
    11701 tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg gagtctttat
    11761 ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg ccagcaaaaa
    11821 acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc catctagcac
    11881 caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc
    11941 caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg cctcgcacgt
    12001 cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca
    12061 ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg
    12121 caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt ggatggcaaa
    12181 acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg gtctggacgc
    12241 ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc tgaaagagaa
    12301 aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag attttaacgc
    12361 tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt aattctctct
    12421 tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata accctgtttc
    12481 agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt aatatcccag
    12541 gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat gattttcggt
    12601 attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt actgcggacc
    12661 ttcgaaaaac gtgccatcgc cagttcacgg ctttggttgc aaatcattac ccagaataaa
    12721 ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca ggcggtattc
    12781 tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca gttgtggatc
    12841 atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa tctggcgcag
    12901 aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat taaactgatc
    12961 ggcgcgattc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc gccagcgatt
    13021 ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtttttaa agatccgatt
    13081 cttggtctgg tggcaggtat tcagctttcc gcgaacgata tgctgaaact gggcgactgg
    13141 ctggagatgc cgaaatacgg cgcggatggc gcggtgatcg atattgggtt aaccaccgtc
    13201 aaagtgcgta actgggacaa taccattacc actattccca cctggtctct ggtttctgac
    13261 tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa gcgcagtatc
    13321 agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg tctgaataaa
    13381 gcgcacttgt taaaacctta tttaaccagc cgccatcagg aaattaatga gtggaatcgc
    13441 cagcaaggtt ctacggagtc gatattaaac ctgcgccgaa tgaccaatat tggaaccttt
    13501 cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga tatgacttta
    13561 atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta tgcgtttacc
    13621 aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga tcacatattt
    13681 gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa tgatattcgc
    13741 tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa tggatgagag
    13801 cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc gttttaaatg
    13861 ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc atatcatcca
    13921 tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcaaagatag ttgccgaacg
    13981 gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct gcacgaaaac
    14041 gcagatgcgc caggcagatc ataatccagt tcaacagcag cgttgctacc accagcgcca
    14101 tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc agcgaagtga
    14161 tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg cgagtcaaaa
    14221 acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta gagtaaaccc
    14281 cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca gaagctacca
    14341 cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat ttcacttcca
    14401 cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc aggatgcgat
    14461 acaccacctg atttaccgct tttggaatgc ttttttccgg atcgcgcgct tcagcggcag
    14521 taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc gacaaaatca
    14581 gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg atactggctt
    14641 tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata ccgatgatcg
    14701 ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc acgttcacca
    14761 ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt ggaacatccg
    14821 ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc accagcacga
    14881 acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag tatttatagg
    14941 caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca agctggcgca
    15001 taatcaggaa agcgatgatc ccggcgacgc cgtagcccag caatacagcc ggacccgcca
    15061 tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca cccagcgcaa
    15121 tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga ttcgacgcag
    15181 tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct tttttgttga
    15241 ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac ggttaaacgg
    15301 ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct tatacgccgc
    15361 cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca tcggtgcggc
    15421 aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca gcagaccggc
    15481 gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc cgtgatacaa
    15541 cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg gctcggcctc
    15601 tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga ccccggcaag
    15661 ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc acagcaacca
    15721 gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt cgcccacccg
    15781 acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca ccatgagttt
    15841 cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca cgctggtgcc
    15901 cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca ccgacaccat
    15961 gtcacgatcg cgggcatcga tataaatcca gctcgtcggg cgcgcattct cggttttcag
    16021 catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga tttgctgctt
    16081 catcggcgtc aggatcggca actggcgcag tgcctgcgga ctatcgcgcc agctttgcgg
    16141 ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa ccatcgcccc
    16201 gcccaccgta gaagtcacaa acaactgcac atccgccacc gtcataccgt aacgcgcggc
    16261 tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct cggcaagcgc
    16321 agaagccacg cctggtaccg ttcgtgccac ctcttcaatt tgctccgcca tcgcgtcgat
    16381 atccgccagc acagtgccgg aaactttaat tccgatgggg cttttaatgc cagtcgagag
    16441 catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg gcagacgcac
    16501 ggtgttatcc agttcctcaa tgattttgtc catcgtcatg cctggccgcc actgctcctg
    16561 cggcttaagc tggatggtcg tttctaccat ctccagcgga gcagaatcgg tggcggtttc
    16621 cgctttcccg gttttcccaa atacccgcgc cacttcaggt acgctcataa ttagcttgtc
    16681 ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg tcgatggcat
    16741 atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt tattgagcgg
    16801 ccagagaacc gtcagcaccg aaagcgccgc caccagcagc gtggttttcg gccagtgcag
    16861 tactttcagc aacagcggat gataaacacg aatcaaaaag cgattgagcg ggttactgct
    16921 ttccggcgga attttgccac ggatccagta gcccatcagg atcgggatca ctacgatcgc
    16981 cagcagcgcc gcacccgcca tcgcatacgt tttggtgaac gccaacgggc caaacagacg
    17041 cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa tcagcagact
    17101 gataaatagc gccggcccca cttcaacaga cgcatcggtg atcacctgcc agcgcgtttt
    17161 attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt tatgcgcatt
    17221 ctcgatcatg acgatagcag catcgaccat cgccccgacg gcaatcgcaa tgccacccaa
    17281 cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa tacacaaccc
    17341 cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa acagcgcaca
    17401 gactaccgcc accacaataa actcttccag caacttgccg ctgaggttgt cgatggcgcg
    17461 gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg gcagactact
    17521 tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt ttttgccgga
    17581 gcgcaggatc accaccccgc ccgccacttc accttcgcca ttcagttcgg caatgccccg
    17641 gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg gcacgccatt
    17701 ttcactggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat agccgctggc
    17761 gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt cctggtttga
    17821 agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca ggcgctgggg
    17881 atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt ccgcaacgtc
    17941 agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta aatcggccag
    18001 atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa cacccgtggc
    18061 atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct ggttgaggta
    18121 ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa tgacatacac
    18181 ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca ccgacaacat
    18241 ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct gaccgggata
    18301 gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca ctggcgtatt
    18361 aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca ccaaaaaacg
    18421 gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca atgcgcatgg
    18481 gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc ggaatcaatc
    18541 aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagcg taatgcggtg
    18601 acgccttgcg atgcctggaa aacagcaacg cgtttcggta caaagcgccc gtcggcatca
    18661 acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg aatgagcagc
    18721 atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt tagcgcctcg
    18781 tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc cacgccaggt
    18841 agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac ggtgagcgta
    18901 aactgcgagg catctttcac cagccaggcg atggactccg ggatcgcagc agtgacccac
    18961 accggatcca taccctgaat tttcgcgacc acgttatctt tggcgatatt cattcccgcg
    19021 cgcagatcaa acgcggtgat cacgccatca atgggcgctt tgagcgtaaa gcgagtctgg
    19081 attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc cagtcgcagc
    19141 cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag cagtaaatac
    19201 tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt gcccttttgt
    19261 actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc gcgagcctgc
    19321 acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg ggcaaaagtc
    19381 agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg agtcgggtca
    19441 atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag atccatatcc
    19501 ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg gtcgtaccag
    19561 aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc ctttgcaaac
    19621 caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat aataagcgcg
    19681 atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg taataaagaa
    19741 aggttgccct gctggacaaa attaaacgcc actttgtcgc cagttttaat ttcactcatt
    19801 ttcgtctgcg gagtgatggt aaagcgcatg gtcatctccg gccagttcac ggcagcaatc
    19861 ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac cacgccagtg
    19921 gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc gttagcctgg
    19981 gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc ttttttcatt
    20041 tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg gtatacaaag
    20101 aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg gttgcaaata
    20161 aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga tataatgccc
    20221 gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg ctgatttgat
    20281 cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg ttctggattt
    20341 tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc agattggcct
    20401 ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc cacatcccgc
    20461 tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg ctggtcaggc
    20521 tgatagacgg gaaaaatgcc gcacgcgcag cgccaatatt ggcattagcc gccattaacg
    20581 cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac aatcccgccg
    20641 gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg cgcttgcggc agcttgccgt
    20701 agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc tgacgtttag
    20761 cgatgtcgct gcgggtactt tcaatcaccc cgcgagcctg ttccagcgcc agaacattac
    20821 tgctacctgt caacagttgt ttttcgacaa acgcatatga ctgctgataa ttacgcagcg
    20881 tttcttcggc tatttgcaat tgcgcatacg ctagctgctg attgaaataa ctttgcgcga
    20941 cattagaaac cagcaaaata tgcaccgcgc gctgggcttc ctcagtggct aaataatttt
    21001 ggcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgagatca aagctggcgt
    21061 taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta ccgctccagc
    21121 tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga cgatattgcg
    21181 cccgcgcttc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc accagcgcct
    21241 cgctaatcag cgtctttacc tgattatcaa caaaaaaggt gcgccagccc acgttctggt
    21301 agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc ggcacgggca
    21361 ttgccggacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg gccacacaaa
    21421 atggcagaag tttacaagga gacataggct cataatttct ggtgatttta taccgccaac
    21481 tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt cattttgcca
    21541 ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat gcggaggaaa
    21601 aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga ccaaagggtt
    21661 aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ttgaatggct accatctggc
    21721 gatgaccggt gattatgatc tgataatcct cgatattatg ctgccggacg tgaacggctg
    21781 ggatatcgtg cgcatgttgc gttccgccaa taaagggatg ccgattctgt tgcttaccgc
    21841 gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg actatctggt
    21901 gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc ggcgcggggc
    21961 ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc tcgtcagccg
    22021 caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta ctctgctgga
    22081 gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct cgcaggtgtg
    22141 ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc ggctacgcgg
    22201 caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg tgggctatat
    22261 gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg gcaacccgtc
    22321 tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca tggatcatga
    22381 tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa gaaattagcg
    22441 ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc ttaatgacgc
    22501 tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat agccacggca
    22561 aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt gacgccatac
    22621 ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg attatgatgc
    22681 caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac ttgccggttg
    22741 gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg atcgattttc
    22801 atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct gtcatcagca
    22861 tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg atccgcagcg
    22921 tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt gacccgcaga
    22981 ccgtgcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc gagcgtattg
    23041 aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa attcgcacgc
    23101 cgattaccaa tctcatcacg caaacggaaa tcgccctcag ccagtcgcgc agccagaagg
    23161 aactggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg aaaatggtca
    23221 gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa aagaaaatgc
    23281 tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta gcggaagatc
    23341 gcggcgtgga gttgcggttt gttggcgaca aatgtcaggt tgcgggcgat ccgctgatgc
    23401 tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca cccagtgagg
    23461 ctattgtagt gcgctgccag acggtcaatc atcaggtgca agtttccgtc gaaaaccccg
    23521 gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctat cgcgttgacc
    23581 cttcccgcca gcgaaaaggt gaaggtagcg gtattgggct ggcgatagtg aaatcgattg
    23641 ttgtcgcgca taaaggcacg gttgcggtaa cgtcagatgc gcgggggaca aggtttgtga
    23701 ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt gcatcacctc
    23761 ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa tatcgaaggc
    23821 ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa tctgtgtttc
    23881 ttccaattgt agatcacaat gccggtgatg ctgccaactt actgatttag
//
LOCUS       DAJSKJ010000051        23639 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000051 DAJSKJ010000000
VERSION     DAJSKJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.051"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..207
                     /locus_tag="ON345_001697"
     CDS             <1..207
                     /locus_tag="ON345_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3071777.1"
                     /translation="NTSQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGG
                     LLDRIDSLIFTAPVFFYFIRYCCY"
     gene            223..1980
                     /locus_tag="ON345_001698"
     CDS             223..1980
                     /locus_tag="ON345_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR3071778.1"
                     /translation="MESSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGLYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLDLIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            1970..3286
                     /locus_tag="ON345_001699"
     CDS             1970..3286
                     /locus_tag="ON345_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR3071779.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSHSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     ILPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(3337..3942)
                     /gene="azoR"
                     /locus_tag="ON345_001700"
     CDS             complement(3337..3942)
                     /gene="azoR"
                     /locus_tag="ON345_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR3071780.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            4143..8045
                     /gene="hrpA"
                     /locus_tag="ON345_001701"
     CDS             4143..8045
                     /gene="hrpA"
                     /locus_tag="ON345_001701"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR3071781.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            8317..9117
                     /locus_tag="ON345_001702"
     CDS             8317..9117
                     /locus_tag="ON345_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3071782.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            9314..10753
                     /gene="aldA"
                     /locus_tag="ON345_001703"
     CDS             9314..10753
                     /gene="aldA"
                     /locus_tag="ON345_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR3071783.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVVMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(10795..11796)
                     /gene="gap"
                     /locus_tag="ON345_001704"
     CDS             complement(10795..11796)
                     /gene="gap"
                     /locus_tag="ON345_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3071784.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSISVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            11985..12515
                     /gene="cybB"
                     /locus_tag="ON345_001705"
     CDS             11985..12515
                     /gene="cybB"
                     /locus_tag="ON345_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR3071785.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12760..12933
                     /locus_tag="ON345_001706"
     CDS             12760..12933
                     /locus_tag="ON345_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071786.1"
                     /translation="MKKLALILVMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(13005..13154)
                     /gene="hokB"
                     /locus_tag="ON345_001707"
     CDS             complement(13005..13154)
                     /gene="hokB"
                     /locus_tag="ON345_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR3071787.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            13553..15193
                     /gene="trg"
                     /locus_tag="ON345_001708"
     CDS             13553..15193
                     /gene="trg"
                     /locus_tag="ON345_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR3071788.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(15231..16154)
                     /locus_tag="ON345_001709"
     CDS             complement(15231..16154)
                     /locus_tag="ON345_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3071789.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            16371..17714
                     /locus_tag="ON345_001710"
     CDS             16371..17714
                     /locus_tag="ON345_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3071790.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            17939..19594
                     /gene="opgD"
                     /locus_tag="ON345_001711"
                     /gene_synonym="mdoD"
     CDS             17939..19594
                     /gene="opgD"
                     /locus_tag="ON345_001711"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR3071791.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            19734..19958
                     /locus_tag="ON345_001712"
     CDS             19734..19958
                     /locus_tag="ON345_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR3071792.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            20021..20557
                     /gene="rimL"
                     /locus_tag="ON345_001713"
     CDS             20021..20557
                     /gene="rimL"
                     /locus_tag="ON345_001713"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR3071793.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(20552..21532)
                     /locus_tag="ON345_001714"
     CDS             complement(20552..21532)
                     /locus_tag="ON345_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR3071794.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            21656..22648
                     /gene="tehA"
                     /locus_tag="ON345_001715"
     CDS             21656..22648
                     /gene="tehA"
                     /locus_tag="ON345_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR3071795.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            22645..23238
                     /gene="tehB"
                     /locus_tag="ON345_001716"
     CDS             22645..23238
                     /gene="tehB"
                     /locus_tag="ON345_001716"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR3071796.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            23541..>23639
                     /locus_tag="ON345_001717"
     CDS             23541..>23639
                     /locus_tag="ON345_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR3071797.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSG"
BASE COUNT         5886 a         5564 c         6167 g         6022 t
ORIGIN      
        1 aatacatcac aggcattatt agcgggtttg ttaattggta ttagtggttt ttgcggcgat
       61 gtagtgatgt cagccatcaa acgagatatt ggtgttaaag atagtggaaa actattacca
      121 ggacatggcg gactccttga caggattgac tcattaattt tcaccgctcc ggtatttttt
      181 tattttatac gctactgctg ttactgaagg aaaatagaga aaatggaaag ttcacgcatc
      241 cctggggaac atttttttac caccagtgat aatacagcgt tgttttatcg gcactggccc
      301 gctttacagc ccggggcgaa aaaggtcatc gtcttatttc atcgcgggca tgaacattct
      361 ggtcgtctac aacatctcgt tgatgaactg gcgatgccag atactgcttt ttatgcctgg
      421 gatgcccggg ggcatggaca aacttcgggg ccgcgtggtt atagcccatc tcttgcgcgt
      481 tcagtgcagg atgtcgatga atttgttcgt tttgctgcca gcgacagcca ggtcggactg
      541 gaagaggtgg tagtgatcgc gcaaagcgtc ggcgcagtgc ttgttgccac atgggttcat
      601 gattatgcac ctgcaattcg tgggctggtg ctggcttctc cggcctttaa ggttaaattg
      661 tatgtgccgc tggcacgtcc tgcgctggcg ttatggcatc gtctgcgtgg tctgtttttt
      721 attaattcct atgtgaaagg gctctatttg acccacgatc ggcaacgggt ggcgagtttc
      781 aataatgatc cgctgatcac acgggcgatt gccgtcaata tcttgctcga tctctataaa
      841 acgtctgaac gtattattag agatgcggcg gcgattacgc tccccacgca acttctgata
      901 tcaggcgatg actatgtggt gcatcgccaa ccgcagattg atttttatca gagattacgt
      961 agccctctga aagagctgca tctgctgcca ggcttttatc acgacacgtt gggtgaagag
     1021 aacagggcgc aggcatttga aaaaatgcaa agctttattc gtcgtttata tgctaacaag
     1081 tcacaaaaat ttgattatca gcatgaagac cgcactggac catcagcgga tcgctggcgg
     1141 ctcctttcag gtggacccgt gccattatcg ccggttgatt tggcgtatcg ctttatgcga
     1201 aaggcgatga aattgttcgg ggcgcactct gcgggcctgc atctcggaat gagcaccggc
     1261 tttgattcag gcagttccct ggattatgtc tatcaaaatc aaccgcaagg cagtaacgca
     1321 ttcgggcgct taattgacaa aatctacctg aacagtgttg gctggcgcgg tattcgccag
     1381 cgtaaaaccc atttacaaat actgattaaa caagccgttg ccgatctcca cgccaaaggt
     1441 ttagccgtcc gcgtggttga cattgccgca gggcatgggc gctatgtact ggatgcgctg
     1501 gcaaacgagc ctgccgtaag cgatattttg ttacgtgatt acagtgagtt aaatgttgca
     1561 caggggcaag cgatgattgc ccaacgggga atgtctgggc gggtgcgttt tgaacagggc
     1621 gatgcgttta acccggagga actcagcgcg ttaactccgc ggcctacgct ggcaattgtc
     1681 tctggactgt atgagctttt tcccgaaaat gagcaggtaa aaaactcact cgcaggtcta
     1741 gccaatgcca tcgaaccggg tggcattctc atctacactg ggcagccgtg gcacccacaa
     1801 ctggatctga ttgccggggt gttaaccagc cataaagatg gtaaaccgtg ggtgatgcgc
     1861 gtgcgttcgc aaggggagat ggattcactc gtgcgtgatg ccggatttga taaatgcaca
     1921 caacggattg atgagtgggg tatttttacg gtttcgatgg cggtgcgtcg tgataactga
     1981 acgtcggaac gtattgctac aaggcgctgg ctggttattg ttgctggccc cgtttttctt
     2041 cttcacctat ggatctctta atcagttcac cgcggttcag gaccttaaca gccatgatat
     2101 ccccagtcag gtattcggtt gggaaacggc gatccctttt cttccctgga ctattgttcc
     2161 ttactggagt ctggatcttt tatatggatt ttcgctgttc gtttgtagca cgacattcga
     2221 acagcgccga cttgtccacc ggcttattct ggcaacggta atggcctgct gcggtttttt
     2281 gctctatccg ctgaagttta gttttatccg tcctgaagtg agtggggtga cgggatggct
     2341 attttcgcaa cttgaactgt ttgatctgcc ttataaccag tctcattcgc tgcatattat
     2401 tctctgctgg ctactttggc gtcactttcg tcagcatctg gctgagaggt ggcgtaaagt
     2461 ctgtggcgga tggtttttac tcatcgctat ttctactctg acgacctggc agcatcattt
     2521 tattgatgtc atcacagggc tggcggtagg tatgttgatt gactggatgg tgcccgtcga
     2581 tcgtcgttgg aattatcaga aacctgatca acgtcgaatc aaaatagcac tgccatatgt
     2641 cgtaggcgcg ggctcgtgca ttgtgttgat ggagctaatg gtgatgattc agttatggtg
     2701 gtcagtctgg ttatgttggc cagtattatc gctactcatt attggccgtg ggtacggtgg
     2761 gcttggcgcg ataacaacag ggaaagatag tcaggggata ctcccgcccg ccgtttactg
     2821 gctgacattg ccctggcgca tcgggatgtg gctgtctatg cgttggtttt gtcgtcgcct
     2881 ggagccggtg agcaaaatta ctgctggtgt ttatttaggg gcgtttccac gacatattcc
     2941 ggcacagaat gcggttctgg acgtcacctt tgaattccct cgggggcgag cgacaaaaga
     3001 tcgactctat ttctgtgtac cgatgctgga tctggtggtt ccggaagagg gggagctccg
     3061 acaggccgtg gcgatgctgg aaacattacg cgaagagcaa ggcagcgttc tggtccattg
     3121 cgcgttggga ttatcgcgca gtgcgctggt agtggcggca tggttgttat gttacggaca
     3181 ctgtaaaacc gttgatgaag cgattagtta tattcgagcc agacgttcgc atattgtgct
     3241 taaggaagat cacaaagcga tgctgaaatt atgggaaaac aggtaagtgg attgagatgt
     3301 ggactgaact acagtccaca tcaagatcgt gtccggttat gcagcaacaa tgctgtcgat
     3361 ggccgctttc gcgtcagact gcgctttcgc tgccatttcc ggaccgtatg cgatcccttc
     3421 ggcgaagaca aatttcacat cggtaatgcc gataaagccg aggaacgtgg acagatacgg
     3481 cgtcaccagg tccgttggtc catctttgtg gatcccaccg cggctggtaa taacgatggc
     3541 ttttttaccc gttaccagac cttccggacc gttctcggta tagcggaaag taacgcctgc
     3601 gcgtgcaacc aggtcaaaat agtttttcaa ctgagttgag atgttgaagt tatacatcgg
     3661 tgccgcaata acgataacgt cgtgggcttt cagctcggca atcagctcat cggaaagcgc
     3721 cagagcttcc tgctgacgcg gagtcagcgg cgcatcgctc ggacgcagag cgccaaccag
     3781 ttcgccatcc agtaccggaa tcggatttgc agccaggtcg cgaacggtga tttcatcagc
     3841 ggagtgcttt tcgctccatt gttcaacaaa ataatcggac aactgattag actgagagta
     3901 ccctgccagg atgctggatt taagaactaa taccttgctc atggtgtttc cttatagatg
     3961 tttgaatggg cgatgccccg ttgcttgctg acactttatt cacaatcctg ccacagagat
     4021 agcgcaataa atcgaagcct atgttcgaat ttattgaaca acgcatagaa agccgcgatg
     4081 tggtactcta tatctatcat ttaaaagaaa attaatcagg cagactactg cccactaacg
     4141 ttatgacaga acaacaaaaa ttgaccttta cggccttgca gcagcggctg gattcgctga
     4201 tgctgcgtga cagactgcgt ttttctcgcc gtctgcacgg cgtgaagaag gttaaaaatc
     4261 ctgatgcaca acaggccatt ttccaggaga tggcgaaaga gattgaccag gcggcaggga
     4321 aagtcctgct gcgtgaagcg gcacgaccgg aaattactta tcctgacaat ttaccggtta
     4381 gtcagaaaaa acaggacatt ctcgaagcga ttcgtgatca ccaggtggtg atcgtcgccg
     4441 gggaaacggg ttctggtaaa acgactcagt taccgaaaat ctgtatggag ctggggcgcg
     4501 ggattaaagg gctgatcggc catacccagc cgcgtcgcct ggcggcaaga acggtggcga
     4561 accgtattgc ggaagagctg aaaacggagc cgggcggttg catcggttac aaagtgcgtt
     4621 tcagcgatca cgtaagtgat aacacgatgg tcaagctgat gaccgacggt atcctgctgg
     4681 cggagatcca gcaagaccgc ctgctgatgc agtacgacac catcattatt gacgaagcgc
     4741 acgaacgcag cctgaatatc gattttctgc tcggctattt gaaagagttg ctgccgcggc
     4801 gtcctgacct aaaaatcatt atcacttccg cgactatcga cccggaacgc ttttcgcgcc
     4861 actttaataa tgcgccgatt atcgaagtct ccggtcggac ctatccggtg gaagtgcgct
     4921 atcgaccgat tgttgaagaa gccgatgaca ccgagcgcga tcagttgcag gcgatttttg
     4981 acgccgtaga cgaactgagc caggaaagcc ctggcgacat tctgatcttt atgagtggtg
     5041 agcgggaaat tcgcgatacc gccgatgcgc tgaacaagct gaacttacgc cataccgaaa
     5101 tcttgccgct ttatgcgcgg ctttcgaaca gcgaacagaa ccgcgtgttc cagtcgcaca
     5161 gcggacggcg cattgtgctg gcgaccaacg tcgcggaaac ctcgctgacc gtaccgggga
     5221 ttaaatacgt catcgacccc ggtacagcgc gtatcagccg ctacagctat cgcaccaaag
     5281 tgcagcgttt gccgattgaa cccatttcgc aggcgtctgc caatcagcgt aaaggccgct
     5341 gtggtcgtgt gtccgaaggg atctgtattc gtctctattc cgaagacgat ttcctctcgc
     5401 gcccggagtt taccgatccg gagattctgc gtaccaacct ggcctcggtt attttgcaga
     5461 tgaccgcgct ggggctgggc gatatcgctg cgttcccgtt tgtcgaagca ccggataaac
     5521 gcaatatcca ggatggcgtg cgtctgctcg aagaactggg cgcgatcacc acggatgaac
     5581 aggccagcgc ctataaactg acgccgctcg gtcgccagct ctcgcagttg cctgtcgacc
     5641 cacgtctggc gcgtatggtg ctggaagcgc aaaaacatgg ctgcgtgcgt gaggcgatga
     5701 ttatcacgtc cgcgctctcc attcaggatc cgcgcgaacg tccgatggac aaacagcagg
     5761 catcggacga aaaacatcgt cgcttccacg acaaagaatc cgacttcctc gcgtttgtaa
     5821 atctgtggaa ttatcttggc gagcagcaaa aggcgctttc ttccaacgcc ttccgtcgcc
     5881 tgtgtcgtac cgattacctc aactatctgc gcgtgcgcga atggcaggat atctacaccc
     5941 agttgcgtca ggtggtgaaa gaacttggca ttccggttaa cagcgaaccg gcggagtatc
     6001 gcgaaattca cattgcgttg ctgacgggtt tactttccca tatcggcatg aaagatgccg
     6061 ataaacaaga atataccggc gcacgtaacg cgcgtttctc catcttcccc ggttctggtt
     6121 tattcaaaaa accgccgaaa tgggtaatgg tggcggaact ggtagaaacc agccgcctgt
     6181 gggggcgcat tgctgcgcgt atcgacccgg aatgggtgga gcctgtcgct cagcatttga
     6241 ttaaacgtac ctacagcgaa ccgcactggg aacgggcgca gggcgcggtg atggcaacgg
     6301 aaaaagtcac tgtttatggt ttgccgattg ttgccgcgcg caaggtcaac tacagccaga
     6361 tcgatccggc gttatgtcgt gaactcttta ttcgccatgc gctggtggaa ggtgactggc
     6421 agacgcgtca cgcattcttc cgtgaaaacc tgaaactgcg ggccgaagtg gaagagctgg
     6481 aacacaaatc acgtcgccgc gatattctgg ttgatgacga aacgttgttt gagttctacg
     6541 accagcgcat cagccacgat gtgatctctg cgcgccactt cgatagctgg tggaaaaaag
     6601 tcagccgcga aacgcctgat ttgctcaact ttgaaaagag catgttgatc aaagaagggg
     6661 cggaaaaaat cagcaagctg gattacccga acttctggca tcagggcaat ctcaagctgc
     6721 gtttgagcta tcagtttgag cccggcgcgg atgctgacgg tgtgaccgta catattccgc
     6781 tgccgttact taaccaggtt gaggaaagcg ggtttgaatg gcagatcccc ggtctgcgcc
     6841 gcgaactggt gattgctctg attaaatcgt tgccgaaacc ggtacgccgt aattttgtgc
     6901 ctgcaccaaa ctacgccgaa gcgtttttag gccgcgtcac accgctggag ttaccgttgc
     6961 tcgacagcct tgagcgcgag ttacggcgga tgaccggcgt taccgttgac cgcgaagact
     7021 ggcactggga tcaggtgccc gatcacctga aaatcacctt ccgcgtggtg gatgacaaaa
     7081 acaagaagct aaaagaaggg cgctcgctac aagatctgaa agatgcgctg aaaggcaaag
     7141 tgcaggaaac gctgtccgcg gtggcggatg acggtatcga gcagagcggc ttacatatct
     7201 ggagttttgg tcagctgccg gaaagttacg aacagaagcg tggcaactat aaagtgaagg
     7261 cgtggccggc gctggtggat gaacgcgaca gcgtggcgat caaactgttt gataacccgc
     7321 tggagcaaaa acaggcaatg tggaatggtc ttcgccgtct actgctgcta aatattccct
     7381 cgccgatcaa gtatttacat gagaagttac caaacaaagc caagctgggg ctgtacttta
     7441 acccgtatgg caaagtgctg gagctgatcg acgactgtat ctcctgcggt gtggatcaat
     7501 tgatcgacgc caatggtggc ccggtctgga cggaagaagg ctttgctgcg ctgcatgaaa
     7561 aagtgcgcgc cgaactgaat gacacggtgg tggatattgc gaagcaggtc gagcaaatcc
     7621 tcactgcggt gttcaatatc aacaaacgtc tgaaagggcg ggtggatatg accatggcac
     7681 tggggctttc tgacattaaa gcgcagatgg gcgggttggt atatcgcggt tttgtcactg
     7741 gtaacggctt caaacggctg ggcgacacgc tgcgatattt gcaggcgatt gaaaaacggc
     7801 tggaaaaact ggcggttgat ccacatcgcg accgtgctca gatgctgaaa gtcgaaaacg
     7861 tccagcaggc gtggcagcaa tggatcaaca aactgccgcc cgcacgtcgt gaggatgaag
     7921 acgtgaaaga gatccgttgg atgatagaag agttgcgcgt tagttacttc gctcaacaac
     7981 ttggtacgcc ttatccgatt tcagataagc gtattttgca ggcgatggag cagattagcg
     8041 gttaaccctg ctatttgcct gataaagaaa aaacccggta agcatttagc gccgggtttt
     8101 ttattaattc taaaacggta agggtaaaaa ttcaggaatt cagaaaaata caattctctg
     8161 ctgcaagatg aataatgttt atctacagca tttccttaaa agatatgtca ggcttgcgga
     8221 gtggcggtta aggacatacg acttcctcct ttcagagtgc tccgcttctc actattatct
     8281 cacgcagtat tcttaaggga acgataagga ggaaccatga acattacccc gtttccgacg
     8341 ctttcgccgg caactataga tgccataaat gttatcggac agtggctggc gcaggatgat
     8401 ttctccggtg aggtgccgta tcaggccgat tgcgtgatcc ttgcaggcaa tgcggttatg
     8461 ccgactatcg atgcggcatg taagattgcc cgcgatcagc aaattccttt actgattagt
     8521 ggtggtatcg gtcactcgac aacttttttg tatagcgcca tcgcacagca tccgcactac
     8581 aacactatcc gcaccactgg cagagcagaa gcgaccatcc tggcggatat cgctcatcag
     8641 ttctggcaca ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa
     8701 aacgcacgct ttagcatcgc gctattgaat caggccgtag aacgagttca tacggctatc
     8761 gttgtacagg accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg
     8821 gacaatcccg atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat
     8881 aacgcagaca gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat
     8941 ctctcactac tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc
     9001 cgcgggcgag attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa
     9061 acgctgaaac atgatgcggt gctcatcgaa gcgatggaaa gtcgctcgtt acgttaaaaa
     9121 ttgcccgttt gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc
     9181 ttttattcct tttacacttg tttttatgaa gcccttcaca gaattgtcct ttcccgatgc
     9241 cgtctctctg atgattgatg ttaattaaca atgcattcac cgaaaacaaa catataaatc
     9301 acaggagtcg cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt
     9361 tacctggcgt ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc
     9421 ccgcataccc gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc
     9481 acaaccagaa tgggaagcgt tgcctgctat tgaacgcgcc agttggttgc gcaaaatctc
     9541 cgccgggatc cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa
     9601 gatccagcag ctggctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc
     9661 ggagtgggca cggcgttacg agggcgagat tattcaaagc gatcgtccag gagaaaatat
     9721 tcttttgttt aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga acttcccgtt
     9781 cttcctcatt gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa
     9841 acctagtgaa tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg
     9901 ccttccgcgc ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact
     9961 ggcgggtaac ccaaaggtcg taatggtcag tatgacaggc agcgtctctg caggtgagaa
    10021 gatcatggcg actgcggcga aaaacatcac caaagtgtgt ctggaattgg ggggtaaagc
    10081 accagctatc gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc
    10141 acgcgtcatt aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg
    10201 catttatgat cagttcgtca atcggctggg tgaagcgatg caggcggttc aatttggtaa
    10261 ccccgctgaa cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag
    10321 ggttgagcaa aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa
    10381 agcggtagag gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga
    10441 aatgtcgatt atgcatgagg aaacctttgg cccggttctg ccggtggtcg catttgacac
    10501 gctggaagag gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta
    10561 tacccaaaat ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta
    10621 catcaaccgt gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg
    10681 tattggcggc gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta
    10741 tttacagtcg taatgagtaa aagaggcgga ggttttttcc ttcgcctgtg cgcgtcagag
    10801 tttagcgaat ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta
    10861 ccaggcgacc gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc
    10921 atcaaacact gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt
    10981 ataaccaaat gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc
    11041 agtcactttt tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac
    11101 gcgttgcgca tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt
    11161 tgccgccccc gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc
    11221 tttaccacgc gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat
    11281 cgtgccgact tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt
    11341 agtggtgcat gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac
    11401 gttataaacg atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc
    11461 agcatcaaga tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat
    11521 gatttctgcc cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccga
    11581 gatacttttc ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg
    11641 accatagttt gaatcatgtt tcagcagata ggcgagaatt tttggggaag tgagatcatt
    11701 aatagcgaca acgtctatgt tgcttttgac ttcaagtaat cgacgcaaca ccagtcgacc
    11761 gatacgacca aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc
    11821 ggatgaaaat tgatcctttc caggcttagc ccaggattgt gggatgggca atctccattc
    11881 ttacagtgaa acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa
    11941 agcccgttat gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact
    12001 caaggttaca aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg
    12061 caatggagtt tcggggtttc ttcccacgta gcgatcggcc actcatcaac atgattcatg
    12121 tttcctgcgg catctcaatt ctcgtgctga tggtcgttcg tcttttgtta aggctgaaat
    12181 acccaacccc gccgattata cctaagccaa aaccgatgat gacgggactg gcgcatttgg
    12241 gacatttggt tatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt
    12301 ataaccgggg caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca
    12361 atttcgaacg ggtagatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt
    12421 ttgtcatcgg gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca
    12481 cacttctacg catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta
    12541 ctaaaaccga cccgtggtac aggcgaagaa tacgggccta catcggaagc gcctattata
    12601 tttatttgta tgacaaataa aaccataatc cttgtccata cgtccatctg gcttatcttt
    12661 aatcaattca cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt
    12721 tcaaatcgaa cataagattc gataaaccat ggaggttata tgaaaaaatt ggcacttatc
    12781 ttggttatgg gagcgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt
    12841 tcgaaagggg ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct
    12901 attagtgcat cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat
    12961 gaatctgcta gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag
    13021 gccatgagcg cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt
    13081 cgggtcagga gtgtgaatgt cagaatcgta atgcagataa tgagcagaca caccaccaga
    13141 gggttgtgct tcatagcctt tctccttgcc ggatggcggg taagaggcta agatctgaat
    13201 tgctaggttc attcgttggc ctcggttgat agaaatatcg gtcggggcct tcgtctttct
    13261 gattcccggt tagcctgaaa acagaaagtc tcaggcaccc gcaggcatcc tatgaggttt
    13321 ccttagggac gaaaataatc acttcacgaa attgcgtgct gttttccaga atttttcgtc
    13381 attcgggtta gcccgtttaa tcattcgtta ctctcttcat tccaatggca ttaaccttct
    13441 ctgcaataat tgttgtaaaa atgtgacgca aagaggcttt tagtcgtaag taattaccgt
    13501 caagtgccga tgactttcta tcaggagtaa acctggacga gagacaacgg taatgaatac
    13561 aactccctca cagcgattag gttttttgca tcacatcagg ctggttccgt tatttgcctg
    13621 cattcttggc ggtatcttag ttctattcgc attaagttct gctctggctg gctatttcct
    13681 ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag attgagattc ggaccgggtt
    13741 agcgaacagt tcagattttt tgcgttcagc ccggatcaat atgattcagg ccggggctgc
    13801 gagtcgtatt gcggaaatgg aagcaatgaa gcgcaatatt gcgcaagccg aatcggagat
    13861 taaacagtcg cagcaaggtt atcgtgctta tcagaatcgg ccggtgaaaa cacctgctga
    13921 tgaagccctc gacactgaat taaatcaacg ctttcaggct tatatcacgg gtatgcaacc
    13981 tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt atcaatcatg aaagtgagca
    14041 gatccgaccg ctggataatg cttataccga tattttgaac aaagccgtta agatacgtag
    14101 caccagagcc aaccaactgg cggaactggc ccatcagcgc acccgcctgg gtgggatgtt
    14161 catgattggc gcgtttgtgc ttgccctggt catgacgctg ataacattta tggtgctacg
    14221 tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg attgaaaaaa tcgccagtgg
    14281 cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa atcggtcgct taagtcgtca
    14341 tttacagcaa atgcagcatt cactggggat gacagtaggg actgttcgac agggcgcgga
    14401 agagatttat cgtggcacca gcgaaatttc agctggcaat gcggacctgt catctcgcac
    14461 cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc atggagcaac tcactgcgac
    14521 ggtgaaacag aatgcggata acgcgcatca tgccagcaaa ctggcgcaag aggcttctat
    14581 taaagccagc gatggcgggc agacggtttc cggtgtagta aaaacgatgg gcgctatctc
    14641 cacgagttcg aagaaaattt ctgagatcac cgccgtcatc aacagtattg ctttccagac
    14701 gaatattctg gcactgaatg ctgccgttga agccgcgcga gcgggtgagc aagggcgtgg
    14761 atttgccgtt gtcgccagcg aagtacggac actcgcaagc cgcagcgctc aggcggcgaa
    14821 agagattgaa ggcttgatca gtgaatcagt caggttaatt gacctggggt cggatgaggt
    14881 ggcaacggca gggaaaacca tgagcactat tgttgatgcc gtcgcgagtg tcacacatat
    14941 catgcaggaa atcgccgccg cctcggatga acaaagtaga ggcataactc aggttagcca
    15001 ggcgatttct gaaatggata aggtgacgca acagaatgct tctctggtag aagaggcctc
    15061 agcagcggcg gtgtcccttg aagaacaggc tgcacgatta actgaggcgg tggacgtatt
    15121 ccgtctgaac aaacattctg tgtcggcaga acctcgcgga gcgggtgaac cagttagttt
    15181 cgctacggtg tgaaaatgtt caaggaggga tcgacagatc ccttcacctt tcagaacggc
    15241 attgattttc gaatagcgtt aatcatcaac tggcaaccag aagagaacgt cgcatctacg
    15301 cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg gaacaggcag ggccaccagc
    15361 gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga caaaccacac gtaatcgtat
    15421 tcaacggtaa gttgacgaga tagcgaagca gacagcgttt cgatacaacc cgaaggaatt
    15481 ttacagccct ggctctgcac taatgcatct gaatgctggc gtggcgcagt gccttctggt
    15541 gatacaacga ccggccattc cagcacccgg cttagcgtta cgttctcctg aagtagcggg
    15601 tgattagggc ggacaaccag cttcaacgat tcaagaaaca gcagttcgta attaagcccg
    15661 gtcatcagtt caggatctga catccgacca atgccgatat cgatttcccc ggtttttaaa
    15721 cccgccagaa tcatagggtt actcattgtc gcaacttgca aggtcgtctc tttttgttgc
    15781 tgatgaaact gacctataac cgaaggtaat atccccagtg ccgcagtagg tagtgcacca
    15841 accctgacga catcattatt aagatcttct ttacgatgaa gcgactgtcc ggcagtgttg
    15901 atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt cgccgggtaa ggtaagttgc
    15961 gccccctgac gaccacgctc aaacaagcga gcgccagtca gctgctccag ttcattcaat
    16021 gtcttagaga gcgcaggttg actcaaatta agggtttcag ccgcgcgccc caaagttcct
    16081 tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac ggatgcgctg actaaacaga
    16141 ctattttttt ccataagcga tgttaaaaac gaagcggtgt cgctgacaag tgaagttgtt
    16201 tgattatgat aacttgattg caaaatatta ttaacaatta aagcaattat gttacagcaa
    16261 aatggataat attgatgttt tcgcggcgag atcacagttt gtaaattctt cccgcaagag
    16321 tgaatgcggt tacctacact ccagattact gaccactgga ggcagacact atggcgaaca
    16381 gcatcacggc ggatgagatt cgggaacagt tttcgcaggc aatgtcagcc atgtaccagc
    16441 aagaagttcc gcaatatggc acgctgctgg aactggtagc tgatgtgaat ctggctgtgc
    16501 tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc agacgagctg gcgcgactga
    16561 atgttgaacg tcatggggcg attcgcgttg ggactgcaca agagcttgct actcttcggc
    16621 ggatgtttgc cattatgggg atgtacccgg tgagctatta cgatctctcg caggcagggg
    16681 tgccggtaca ttcgacagca tttcggccca ttgatgatgc ttctctggcg cgtaatccct
    16741 tccgcgtttt tacctcctta ctccgccttg agcttatcga gaacgaaatt ttgcgccaga
    16801 aagcggcgga gattctgcgt cagcgcgata tcttcacccc acgttgtcga caactgttag
    16861 aggaatatga gcagcagggc ggttttaacg aaacacaggc acaggagttt gtgcaggaag
    16921 ccctggaaac gtttcgctgg caccagtcag caacggtaga tgaagaaacc tatcgcgcct
    16981 tgcacaacga acatcggttg attgctgatg tggtctgttt tcctggatgc catatcaacc
    17041 acctgacgcc acgtacgctg gatattgacc gggtgcagtc gatgatgcct gaatgcggaa
    17101 ttgaacccaa aattctcatc gaggggccgc cgcgccgcga ggtaccgatt ttactacgcc
    17161 agaccagctt taaagcactg gaagagacgg tgttgtttgc ggggcagaaa cagggcacgc
    17221 ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc attaacgccg aaagggcgac
    17281 aactgtatga tgatcttctg cgtaacgctg gaaccgggca ggataatctc actcaccaaa
    17341 tgcatttaca ggaaaccttc cgcacttttc ctgacagtga gtttttaatg cgtcagcaag
    17401 ggttggcatg gttccggtac cgtctgacgc cttcgggtga ggcgcatcgt caggcgattc
    17461 atcctggaga cgatccacag cccttaattg aacgtggttg ggtagtggcg caacccatca
    17521 cctatgaaga tttcttgccc gttagcgcgg cggggatctt ccagtcaaat ctgggtaatg
    17581 aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc atttgagcag gcgttgggtt
    17641 gtccggtttt ggatgagttc cagctttacc aggaagcgga agaacgcagt aaacgtcgct
    17701 gtggtttgct ttaaattctg accatccgcc tttgcaaaaa tttgcctgat tttacaaacg
    17761 aatcaggctc atcccatcga cataaaaaaa atgccgattt atgcatattc tctcagttca
    17821 acaattggat tattaataaa tattgtctag agtgagcggt cataaataag cactttcttg
    17881 ccgctgaaaa cgaccagcgc gggaccattc acaacaccag aaggactcac tttcaggtat
    17941 ggatcgtaga cgatttatta aaggttcaat ggctatggcc gccgtgtgcg gtaccagcgg
    18001 cattgcttct cttttttctc aggcggcatt cgcggcagat tctgatattg ccgacgggca
    18061 aacccagcgt tttgacttct ccattctaca gtcaatggcg cacgacttag cgcaaacagc
    18121 gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg acaatgacgc cgcaggctta
    18181 taacagtatt caatacgacg ccgaaaaatc gctctggcat aacgttgaga accgtcaact
    18241 ggacgctcag ttcttccata tgggaatggg attccgtcgc cgcgttcgta tgttttctgt
    18301 agatccagca acacatctgg cgcgtgaaat tcactttcgc ccggagttgt tcaaatacaa
    18361 cgatgcgggt gttgatacaa aacaattaga agggcaaagc gatctcggct ttgccggttt
    18421 tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta gtatcatttc tcggcgcgag
    18481 ttatttccgc gccgttgatg atacatatca atacggtttg tcggcccgcg gcctggcgat
    18541 cgacacttac accgacagta aagaagagtt ccccgacttt accgccttct ggtttgatac
    18601 ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg ctcgatagcg ccagcattac
    18661 tggtgcctat aagttcacta tccattgtga gaaaagtcag gtgattatgg atgtggaaaa
    18721 tcacctgtat gcgcgcaaag acattaaaca gctgggcatt gcgccgatga ccagtatgtt
    18781 cagctgcggt actaatgaac gtcggatgtg cgatacaatt catccgcaaa ttcatgactc
    18841 tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt tgccgtccgc tgaataatcc
    18901 gcaaaaattg cagttcaatg cttacaccga caacaacccg aaagggtttg gtttattgca
    18961 actggatcgt gatttctccc attatcagga cattatgggc tggtataaca aacgcccaag
    19021 tctgtgggtg gagccgcgta acaagtgggg taagggcacc atcggcctga tggaaatccc
    19081 aacaacgggt gaaacgctgg ataacattgt ctgcttctgg cagccagaaa aagctgtaaa
    19141 agcaggtgat gagtttgcat tccagtatcg tctgtactgg agtgcgcaac cgcctgttca
    19201 ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg ggcggtttcc cggaaggttg
    19261 ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt tttgccgtcg atttcgttgg
    19321 tggtgatctg aaagctgccg cgccaaaagg cattgagccg gtgattacgc tttccagtgg
    19381 ggaagcgaag caaatcgaaa ttctctatat tgaacccatc gatggttatc gtattcagtt
    19441 tgactggtat ccgacttcgg actccactga tccggtcgat atgcggatgt atctacgttg
    19501 tcagggggac gctatcagtg aaacatggct gtatcagtat ttcccgccag cgccggataa
    19561 acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt tttcttcggc accttcttcg
    19621 ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt tccctaagtt aaatgagtaa
    19681 tctgatggtg tgtatttcag atacaccttg tcagccacta acagggagtg cgtatgtttc
    19741 cagaataccg agatttaata tcccgtctga aaaacgaaaa tcctcgcttt atgtccttgt
    19801 tcgataaaca caataaactt gatcatgaaa ttgccagaaa ggaaggttcc gacggtcgag
    19861 ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct acagttaaaa gatgagatgc
    19921 tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac tttcctaagc cgccagccag
    19981 gcggcttttt taacaacagc atggattgac tggagataag atgactgaaa cgataaaagt
    20041 aagcgaatca cttgaattac atgctgttgc ggaaagtcac gtcacacctc tttatcagtt
    20101 aatctgtaaa aataaaacct ggttacagca gtcgctaaac tggccgcagt tcgttcagag
    20161 tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg ttgcatcaac gcggctatgc
    20221 caaaatgttc atgattttca aagaagatga acttatcggc gttatctcgt ttaatcgtat
    20281 tgaaccactg aataaaactg ctgaaatcgg ctactggctg gacgaatctc atcaggggca
    20341 ggggatcatt tctcaggcgc tgcaggcatt gattcatcat tacgctcagt ctggtgaact
    20401 tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa agcaaccagg tcgctttgcg
    20461 caatggtttt atccttgaag gttgcctgaa acaggctgag ttcctgaatg atgcctatga
    20521 tgatgtgaac ttatacgcgc gtattatcga ttcataataa ccctgccagc ggcgttcgcg
    20581 taatgcgctc ctcgccgtta atgacttttg gaccacggac atgaaccgtg tcgccatcaa
    20641 aggcttcaac gacggcgtgg tcggttaact ccacatggtt ttcaattatc accgcacctg
    20701 aaatacggct ctcaccttgt attacaacgt gctcgtcgag cagaatcggt ccaccacgta
    20761 ccacggcatt tccgccaatc aagacatgat gttttaacac acaattacct tccacaatgg
    20821 catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat ggcatcttct tctatgccag
    20881 ctttcacctg cgcgtgaccg tagactttag cgcaatcgca tagccagacg ttgttctctt
    20941 cattaccttc aacactggca aaatcaaaaa cttcggcgcg atgttcaata aaagcatacc
    21001 ggacgacggc atcgccataa atttgtgcct ggtggacaat acgcgaggcg ctaacccttg
    21061 cgcgatcata aatttggagc aggagttgat ggtcgggcgt taagccttgt gcggcgacga
    21121 tcatagaatg ttgatcaatt aaggcgtgac caaatattct acattgcccg taaactaatg
    21181 agtcatgaat ggtgacactg tcacttatat atgcaccctg gctgatttca ctattatcga
    21241 tccagacgtt atccgttgcg taaacctctc cccacagcac gctggtgccg gtgatacgcg
    21301 tattaccaga aatcaccgtt ccgccgaacg caatagcatt ctggtcgtaa atccagcaat
    21361 ttccttcctg tgctaacacc gtttcgcgat cgatccagcc gccagcggtt cctgctatca
    21421 catcgttaaa atcgctaatg gcgataatct gccgtaataa cacacttttt ttagtgccat
    21481 cttcctgata actaaaggct cgctgttctt cacttagacg atatttgcgc atagcatttt
    21541 ctcacaggtg acttactata accgtagcaa attctgcggc tctggctatg ctcgagaaaa
    21601 ttccataaaa tgcatttcaa atatacttta taaattaaac aaaatgagta agaagatgca
    21661 gagcgataaa gtgctcaatt tgccggcagg ctactttggt attgtgttgg ggacgatagg
    21721 gatgggattt gcctggcgct atgccagcca ggtttggcag gtcagccact ggttagggga
    21781 tgggctggtg attctggcga tgatcatctg gggattatta actagcgcat ttattacccg
    21841 actcatacgc tttccgcata gcgtgctggc ggaagttcgc catccagtgc tgagcagttt
    21901 tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt ggttttgttc cgtggtttcg
    21961 cccactggcg gtgtgcctgt tcagttttgg tgtcgtggtt cagttggctt atgccgcctg
    22021 gcaaactgcg ggattatggc gcggatctca ccctgaagaa gctaccacgc ctggactgta
    22081 tctgccgaca gttgccaaca actttatcag cgcaatggcc tgtggtgcgt tgggctacac
    22141 cgacgccggt ctggtgtttt taggcgcagg cgttttctca tggctaagcc ttgaaccggt
    22201 gatcttgcag cgtctgcgca gttcgggaga attacccacg gcactgcgga catcactcgg
    22261 cattcagctc gctcctgcgc tggtggcctg tagtgcctgg ctgagcgtca acggcggcga
    22321 gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg ctgcaactgc tgtttatgct
    22381 acgtctgatg ccatggtatc tctcccagcc atttaatgct tcattctgga gtttctcgtt
    22441 cggcgtatct gcactggcaa ccaccggttt gcatctgggg agtggcagcg ataatggatt
    22501 tttccatacg ctggcggtgc cgctgtttat ctttaccaat tttattattg caatactgct
    22561 catccgtact tttgcgcttc tgatgcaggg aaaattgtta gtcagaaccg agcgcgccgt
    22621 tttaatgaaa gcagaggaca aagaatgatc attcgtgacg aaaactattt tactgataaa
    22681 tatgaattaa cccgcacaca ctctgaagta ctggaagcgg tgaaagtggt taaaccgggt
    22741 aaaacgctgg atctgggctg tggcaatggt cgtaacagtc tttacctggc agccaatggt
    22801 tatgatgttg acgcatggga taaaaatgcc atgagtatcg ccaacgtcga gcgcattaaa
    22861 tccattgaaa atctggataa tttacacacc cgagtcgttg atctgaataa cctcacattt
    22921 gatagacagt acgattttat tctttcgact gtggtactga tgttccttga ggctaaaacc
    22981 atcccagggc tgattgccaa tatgcaacgt tgcactaaac ctggtggtta caacctgatt
    23041 gtggcggcga tggataccgc tgattatcca tgtaccgtcg gcttcccgtt tgccttcaaa
    23101 gagggagaat tacgtcgata ttacgaaggc tgggagaggg tgaaatacaa tgaagacgtc
    23161 ggcgagctgc accgcaccga cgccaacggt aatcgtatta aactgcgttt cgccacgatg
    23221 ctggcacgta aaaaatgacc cgctaagaat aaggcgcgtg aaaattcccc acgctgagat
    23281 gatttactgt tcttcttttc ggtaagcata ttttttatcg aagggatgtg aaattaatca
    23341 cagtagtcga agttttttag cagcttaact tactgaaatt taactactga tgattgactt
    23401 agcccctttt tcggcattga ctatgtcgtc tgaaaagggg ctgaaaaatt tattttcacc
    23461 aacacttttt ttgccacaac acgaagcggt gctttttgct ataacttaaa aagtaatata
    23521 atcatctcag gaaactattc atgcgtacca catcatttgc gaaagttgca gctttatgcg
    23581 gcttgttggc tctgtctggt tgtgcctcta aaatcaccca gccagataaa tattctggt
//
LOCUS       DAJSKJ010000052        23354 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000052 DAJSKJ010000000
VERSION     DAJSKJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.052"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(50..817)
                     /locus_tag="ON345_001718"
     CDS             complement(50..817)
                     /locus_tag="ON345_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3071798.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1013..1921
                     /locus_tag="ON345_001719"
     CDS             1013..1921
                     /locus_tag="ON345_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3071799.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1918..3180
                     /locus_tag="ON345_001720"
     CDS             1918..3180
                     /locus_tag="ON345_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HCR3071800.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3177..3815
                     /locus_tag="ON345_001721"
     CDS             3177..3815
                     /locus_tag="ON345_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR3071801.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            3820..4596
                     /locus_tag="ON345_001722"
     CDS             3820..4596
                     /locus_tag="ON345_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR3071802.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4685..6049
                     /locus_tag="ON345_001723"
     CDS             4685..6049
                     /locus_tag="ON345_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR3071803.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6089..6325)
                     /locus_tag="ON345_001724"
     CDS             complement(6089..6325)
                     /locus_tag="ON345_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071804.1"
                     /translation="MICPRCADEQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(6336..7763)
                     /locus_tag="ON345_001725"
     CDS             complement(6336..7763)
                     /locus_tag="ON345_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR3071805.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            complement(7763..8356)
                     /locus_tag="ON345_001726"
     CDS             complement(7763..8356)
                     /locus_tag="ON345_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR3071806.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            8503..8910
                     /locus_tag="ON345_001727"
     CDS             8503..8910
                     /locus_tag="ON345_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3071807.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(9031..10023)
                     /gene="rpoS"
                     /locus_tag="ON345_001728"
     CDS             complement(9031..10023)
                     /gene="rpoS"
                     /locus_tag="ON345_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR3071808.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(10086..11225)
                     /gene="nlpD"
                     /locus_tag="ON345_001729"
     CDS             complement(10086..11225)
                     /gene="nlpD"
                     /locus_tag="ON345_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR3071809.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(11365..11991)
                     /gene="pcm"
                     /locus_tag="ON345_001730"
     CDS             complement(11365..11991)
                     /gene="pcm"
                     /locus_tag="ON345_001730"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR3071810.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(11985..12746)
                     /gene="surE"
                     /locus_tag="ON345_001731"
     CDS             complement(11985..12746)
                     /gene="surE"
                     /locus_tag="ON345_001731"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR3071811.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(12727..13776)
                     /gene="truD"
                     /locus_tag="ON345_001732"
     CDS             complement(12727..13776)
                     /gene="truD"
                     /locus_tag="ON345_001732"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR3071812.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(13773..14252)
                     /gene="ispF"
                     /locus_tag="ON345_001733"
     CDS             complement(13773..14252)
                     /gene="ispF"
                     /locus_tag="ON345_001733"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR3071813.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(14252..14962)
                     /gene="ispD"
                     /locus_tag="ON345_001734"
     CDS             complement(14252..14962)
                     /gene="ispD"
                     /locus_tag="ON345_001734"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR3071814.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(14981..15292)
                     /gene="ftsB"
                     /locus_tag="ON345_001735"
     CDS             complement(14981..15292)
                     /gene="ftsB"
                     /locus_tag="ON345_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR3071815.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(15486..15809)
                     /locus_tag="ON345_001736"
     CDS             complement(15486..15809)
                     /locus_tag="ON345_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR3071816.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(15859..16464)
                     /gene="cysC"
                     /locus_tag="ON345_001737"
     CDS             complement(15859..16464)
                     /gene="cysC"
                     /locus_tag="ON345_001737"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR3071817.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(16464..17891)
                     /gene="cysN"
                     /locus_tag="ON345_001738"
     CDS             complement(16464..17891)
                     /gene="cysN"
                     /locus_tag="ON345_001738"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR3071818.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(17893..18801)
                     /gene="cysD"
                     /locus_tag="ON345_001739"
     CDS             complement(17893..18801)
                     /gene="cysD"
                     /locus_tag="ON345_001739"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR3071819.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            19053..20090
                     /gene="iap"
                     /locus_tag="ON345_001740"
     CDS             19053..20090
                     /gene="iap"
                     /locus_tag="ON345_001740"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR3071820.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   20111..20323
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20172..20201
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(20419..20712)
                     /gene="cas2"
                     /locus_tag="ON345_001741"
     CDS             complement(20419..20712)
                     /gene="cas2"
                     /locus_tag="ON345_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR3071821.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(20712..21632)
                     /gene="cas1e"
                     /locus_tag="ON345_001742"
     CDS             complement(20712..21632)
                     /gene="cas1e"
                     /locus_tag="ON345_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR3071822.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFGSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(21629..21727)
                     /locus_tag="ON345_001743"
                     /pseudo
     CDS             complement(21629..21727)
                     /locus_tag="ON345_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
     gene            complement(21724..23354)
                     /gene="cas3"
                     /locus_tag="ON345_001744"
                     /pseudo
     CDS             complement(21724..23354)
                     /gene="cas3"
                     /locus_tag="ON345_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT         5506 a         6337 c         5892 g         5619 t
ORIGIN      
        1 ctcatgcttc ttctgtgaag catgagtaac caaatgttat tgcaggccat tattcatttt
       61 tcgctaccag caaagagaga tcctgcttcg ccagcgcgcg actggcactc tccggcaaac
      121 cgtcgtctgt aataatctga tcaaactcgc ttaatggtaa cgccagccat gtcgccacct
      181 gaccatattt cgtcgcatca cagaccaaaa ctcgctggcg gctggcactg gcaatcgccc
      241 gtttcaccgt gactttatct tccgctggcg tagaaatccc ccgcacactc catgacgatg
      301 cagaaataaa agcctgatca atcatcaggc tgcgcagcat ggtcgcagcg gcttccccga
      361 cacaggaacg gttttcccga cacactgcac cgccagtgtg aataattgtg caattactgt
      421 tgtcgagcaa gtagttcgca ataacgaaat cgtttgtgac cacagtcagt gactccatgt
      481 gaatcagatg ctgtgctatc gctaacgtgg tcgttcccgc atccagatag atacaacttc
      541 ccggctgaac aagacttgcc gccagcttgc caatagccgc tttttgcgtc attgccagcg
      601 cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc cggagactgg acgcctccgg
      661 acaccagcac aacggctccc tgctgctcca gtttttgtaa atcccgacga atggtcatat
      721 gtgacacatt cattctgtcc gttagttcag caatactgac aatgcctttt tcagctacca
      781 tctcaaggat gatttggcga cgctctacgg gtatcaactt ttgctccttc ctttgtcctg
      841 ctgacattct acgctatttg cctgcgaaac gtgcgcggcg caactaacgc ttagttcaca
      901 taaaataaca cacaatgtta atttatgtga atcagctcac catatcgtta tcttccagcg
      961 cttatattca caatatcaaa caaaatatca cttaaattaa caaggagagc agatgaaaac
     1021 gggatctgag tttcatgtcg gtatcgttgg cttagggtca atgggaatgg gagcagcact
     1081 gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac ctgaacagca atgcctgcgc
     1141 tacgttgaaa gaggcaggtg cttgcggggt ttctgataac gccgcgacgt ttgccgaaaa
     1201 actggacgct ctgctggtgc tggtggtcaa tgcggcccag gttaaacagg tgctgtttgg
     1261 tgaaacaggc gttgcacaac atctgaaacc cggtacggca gtaatggttt cttccactat
     1321 cgctagtgct gatgcgcaag aaattgctac cgctctggct ggattcgatc tggaaatgct
     1381 ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac ggtgaaatga ctgtcatggc
     1441 ctccggtagc gatattgcct ttgaacgact ggcacccgtg ctggaagccg ttgccggaaa
     1501 agtttatcgc ataggtgcag aaccgggact aggttcgacc gtaaaaatta ttcaccagtt
     1561 gttagcgggc gtacatattg ctgccggagc cgaagcgatg gcacttgcag cccgtgcggg
     1621 gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc gccggaaatt cctggatgtt
     1681 cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc ccgcattcag ccgtcgatat
     1741 ttttgttaag gatcttggtc tggttgccga tacagccaaa gccctgcact tcccgctgcc
     1801 attggcctca acagcattga atatgttcac cagcgccagt aacgcaggtt acgggaaaga
     1861 agacgatagc gcagttatca agattttctc tggcatcact ctaccgggag cgaaatcatg
     1921 atcaagattg gcgttatcgc cgatgatttt accggcgcga cggatatcgc cagttttctg
     1981 gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc caacaggtaa aatgccggaa
     2041 gcaatcgacg cactggtgat cagcctgaaa acgcgctcct gtccggtggt tgaagccaca
     2101 cagcaatcgc tggcggctct gagctggttg caacagcaag gttgcaaaca gatctatttc
     2161 aaatactgct ctactttcga cagtacggcg aaaggtaata tcggcccggt taccgatgca
     2221 ttaatggatg ctctcgacac gccgtttacg gtcttctctc cggccctgcc ggtcaacgga
     2281 cgtacggttt atcaggggta tttgttcgta atgaatcaac tgctggccga atccgggatg
     2341 cgccatcacc cggtaaatcc catgaccgac agctatcttc cccgtctggt tgaagcgcaa
     2401 tccacagggc gctgcggcgt cgtttcggca catgttttcg aacaaggtgt ggatgccgtt
     2461 cgtcaagagc tggctcgctt acagcaagag ggctaccgct acgcggtgct tgatgcgctg
     2521 accgaacacc atctggaaat tcagggagaa gccttgcgcg atgccccact ggtaacgggc
     2581 ggttctggtc tggcgattgg cctggcccgg cagtgggcgc aagaaaacgg taaccaggct
     2641 cgcgaagcag ggcatccgct cgctgggcgc ggcgtagtgc tctccggttc atgctctcaa
     2701 atgaccaacc gccaggtggc acattaccgt caaattgcac cagcccgtga agttgatgtg
     2761 gcacgctgcc tctcaaccga aactctggcc gcttatgcac acgaactggc agagtgggtt
     2821 ctgggccagg aaagtgtact tgctccactg gtttttgcca ccgccagcac tgacgcattg
     2881 gcagcaattc aacagcaata cggtgcacaa aaagccagtc aggcagtaga aactctgttt
     2941 tctcaactag cggcgcggtt agcagcggaa ggcgtgacac gctttattgt cgcaggcggt
     3001 gagacctccg gcgtagtcac acagagcctg ggcataaaag ggtttcatat tggcccaacc
     3061 atttcccccg gcgtgccgtg ggtaaacgca ctggataagc ctgtctcact cgcccttaaa
     3121 tctggcaact tcggtgatga agcctttttt tcacgagccc aaagagagtt tttatcatga
     3181 gcgatttcgc aaaagtagag cagtctttgc gagaggagat gacgcggatt gccagttcat
     3241 tctttcagcg cggctatgca accggttcgg ctggcaatct gtcgctgctt ttacctgacg
     3301 ggaatttact ggcgacaccg acaggttcat gcctgggcaa tctcgatccg cagcggcttt
     3361 ccaaagtcgc cgcggatggc gaatggttaa gtggtgacaa accctcgaaa gaggtgctct
     3421 ttcatctggc gctgtatcgc aacaatccgc gctgtaaagc ggtggtgcat ttgcacagca
     3481 catggtcgac ggcgctttcc tgcctgcaag ggctggacag cagcaacgtt attcgtccgt
     3541 tcacaccata cgtggtgatg cggatgggaa atgtcccgct ggtgccttat taccgaccgg
     3601 gcgataaacg catcgcacag gatctggcgg aactggcagc agacaatcag gcttttttac
     3661 tggcaaatca tggcccagtg gtttgcggtg aaagcctgca agaagccgcc aacaatatgg
     3721 aagagctgga ggaaacggca aagctgattt ttattctcgg tgaccgcccg atccgttatc
     3781 tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa tgcctcgttt tgcagctaat
     3841 ttatccatga tgttcaccga agtgcctttt attgaacgct tcgccgcagc gcgaaaagcc
     3901 gggttcgatg ctgtggaatt tctgtttccc tatgactact ccaccctgca aatccaaaag
     3961 caactggagc aaaaccatct gacactggcg ctgttcaata ccgctcccgg agatattaat
     4021 gcgggggaat ggggattatc cgcccttccc ggacgtgagc acgaagcaca cgcagacatc
     4081 gacctggcac tggaatatgc attggcgctc aattgtgaac aagtccatgt gatggcaggc
     4141 gtcgtgcccg ctggcgaaga cgctgaacgg taccgggcag tatttatcga taatctccgc
     4201 tacgctgccg accgctttgc gctacacggc aagcgaatat tagttgaagc actcagcccc
     4261 gacgttaagc ctcattatct cttctccagc cagtatcagg cactggctat tgttgaagag
     4321 gttgcgcgag ataacgtgtt tattcaacta gatacttttc atgcgcaaaa agtagatggc
     4381 aacctgacgc atttaatccg cgactacgct gggaaatatg cgcacgtaca aattgccgga
     4441 ctacctgatc gacatgaacc ggacgatgga gaaatcaact atccgtggct gttccgcctg
     4501 ttcgatgagg tgggatatca gggctggatc ggttgtgaat ataaacctcg tggcctcacc
     4561 gaagaagggc ttggctggtt tgacgcctgg cgctaattcg ttaaatccct aattacaacg
     4621 tacccataca tccccccatc tacggatggg gtaggttaac tataactttc agacagggtt
     4681 tcccatgtcc acaattacat tgttatgcat tgcgttagct ggcgtaatca tgctgttgct
     4741 gctggtcatc aaggcaaagg tacaaccatt cgttgctctg ctcctcgtca gcctgttagt
     4801 cgcacttgcg gcgggtatac cggcgggcga agtgggtaaa gtgatgatcg ccgggatggg
     4861 cggtgtgctt ggttccgtca ctattattat tggtctgggc gctatgctgg ggcgtatgat
     4921 cgaacactct ggtggtgcag agtcactggc taattatttc agtcgcaagt taggtgacaa
     4981 acgaactatc gctgcgctga ctctggcagc gttcttcctc ggtattcccg tcttctttga
     5041 tgtcggcttt attattcttg cgccaatcat ttacggtttt gccaaggtag ccaaaatatc
     5101 gccactcaaa tttggcctgc ctgtcgctgg gatcatgctc actgttcacg tggcggtacc
     5161 gccgcatcca ggccctgtcg ccgcagcggg gttactccac gcagacatcg gctggctaac
     5221 catcatcggt attgcgattt ctattcccgt aggggttgtt ggctactttg cagcgaaaat
     5281 aatcaataaa cgccaatatg cgatgtcggt ggaagtactg gaacagatgc aactggctcc
     5341 ggccagtgag gaaggcgcga caaaattaag cgataaaata aatccaccgg gcgtcgcgct
     5401 ggtcacctcg ctaattgtta ttcctatcgc gattatcatg gcgggtacgg tttccgcaac
     5461 actgatgccg ccttcgcatc ccctgcttgg tacgctacag ctgatcggct caccaatggt
     5521 agctctaatg attgcgctgg tgctggcatt ctggttattg gctttacgtc gcggctggag
     5581 cttacaacac accagcgaca ttatgggctc agcccttccc actgcggcgg tagtgatttt
     5641 ggttactggt gctggagggg tatttggcaa agtgctggtg gaatcgggcg tcggcaaagc
     5701 ccttgccaac atgctacaaa tgattgacct gcctctgtta ccagccgcat ttattatttc
     5761 actggcgctg cgtgcatcgc aggggtcagc aaccgtagca atcctgacaa ccggcgggtt
     5821 actctcagaa gcggtgatgg gattgaatcc gattcagtgc gtattggtga cgctggcagc
     5881 ctgctttggt gggcttggcg cgtcacatat taatgactca gggttctgga ttgtgaccaa
     5941 atatctgggg ttgtcggtag cagacggtct gaaaacctgg acagtgttaa cgaccattct
     6001 cggttttacc ggattcttaa ttacctggtg cgtatgggcg gtaatttgac cttaataaaa
     6061 aggtccgatg ggcatcggac ctttttattt agcgcttacc ttccgccagc agcggcggga
     6121 tgctcggcac cattggcgca tcatcaatat ctttctgcgt catgcggaac gcttccgggt
     6181 aatgttcgcg actggtgcgg cgtagcggtt cggtatcgcg ccaggtataa aggcaatgct
     6241 ggcactgata taccgtccag acatctttca ccggcgattt caccatcact tcaatctgtt
     6301 catcggcaca acgtggacaa atcatcttgt tctccttatt tacgtgcagc cagcatagtg
     6361 gtcagttttt cagcccaggc tttggtttcc ggcaaatcca ccaccggctg gctgtagtga
     6421 ccacggttgt ccggggcgac aggcgtggta gcgtcgataa tcagcttgtc ggtgatcccc
     6481 gccgggcttg agccagggtc gagttccagc acggacatat tcggcaactg caccaaatcc
     6541 cctgccgggt ttactttcga ggagagcgcc cacatcacct gcggcaggtt gaacgggtca
     6601 acgtcttcat cgaccataat caccatcttt acgtagccca gaccgtgcgg cgtggtcatc
     6661 gcacgcaggc ccaccgcgcg ggcaaagccg ccgtagcgtt ttttggtgga gataatcgcc
     6721 agcaggccgt gggtgtacat ggcatttacc gcctgcactt ccgggaactc ggctttcagt
     6781 tgttgataca gcggtacgca ggtggctggc cccatcaggt agtcgatttc ggtccacggc
     6841 ataccgagat agagtgattc gaaaatcggt ttgctgcgat aagagacttt atcgatgcgc
     6901 accacggtca tgttgcgccc accggagtag tgcccggtaa actcaccgaa cggcccttcg
     6961 atttcgcgtt tacggctttc gataacccct tcgaggatca cttctgaacc ccacggcaca
     7021 tcaaaaccgg tcagcggggc ggtggcgatc gggtacgggc tttcacgcag cgcgcctgcc
     7081 atttcatact cagactgatc gtatttcagc ggcgtggccc ccatcagcgt gatgatcgga
     7141 tcgttaccga gcgtgatcgc aatcggcaga tcttcaccgc gctcttctgc tttatgcaga
     7201 tgcagggcga tatcgtgcat cggcaccggt tgcaggccga gcttacgctt gcccttcact
     7261 tccatgcggt agatgccgac gttctgcttg ccgaagttat ccgggtcgag cggatcgcgg
     7321 gaaaccacgc acgctttgtc gagatagaaa ccgccatcgc catcgtttaa acgaaacagc
     7381 ggcaggatat cgaacaggtt aatctcttcg ccatcgacgg tgttctgcgc ccaggctgga
     7441 ttggcgcggc gctccggggc aatcgggaag ttatcccagc ggcggataaa ctcatcaatc
     7501 tgctttttga ccggggtgtt tggtggcagg ccaagggaaa tcgcgtggtt ctgccaggaa
     7561 ccgatggtat tcatcgccac gcgggcatcg gtaaagccgc gaatattatc aaaccacagc
     7621 gcgggcgcgc cgtcgccgat acgcccggtg gcgttagctg ctgctgccag atccggctca
     7681 gcgttcacct cttcgctgat tttcagtaac tggccgtggt catcaagcgc ctgtaaaaag
     7741 ctgcgtaaat catcaaatgc cattattcat tctcctgaga aaaattccgg gcctgcggca
     7801 atccttgcca gcgcctggcg tggggatgtt cgaggccaaa ttgatccagc acgcgggcta
     7861 ccacatggtg gacaatgtca tctaccgttt cgggatggtt ataaaaggca ggcatcggcg
     7921 gcaccatcgc cacgcccatg cgtgaaagtg cgagcatatt ttcgagatgg atggtgctaa
     7981 gcggcatttc tcgcggcacc agcaccagtt tgcggccttc tttgagcacg acgtccgccg
     8041 cgcgccctac caggccatcg gcgtaaccag cgcggatacc ggcgagcgtt ttcatactgc
     8101 acggaataac gatcatgccg tcggtacgaa aagaaccgga tgagatgatc gccgcctgat
     8161 ccgccgggtt atggctgaag tctgcgaggg cagcaacatc gcgagcgctg taaggcgttt
     8221 ccagttcaat ggtggttttc gcccacttcg acatcaccag atgagtctcg acattcggca
     8281 tctcccgcag cgcttgcagt aatgccacac caagaggcgc accggtagcc cctgtcatcc
     8341 cgacgatcag tttcattgct tattctctta attattcgta tacgaacgtt attattaaca
     8401 tactccttca tttccacagc ggcaaggatt gaaaagtaac aacatcttct tcgtcactaa
     8461 aaaaccgcta tgatagcgac agagaattag cccggagttt acatggcgtt acgaaataaa
     8521 gcgttccatc agttacgaca gctttttcag cagcacacgg ctcgctggca gcacgagtta
     8581 cctgacctga ccaaaccaca gtacgcggtg atgcgcgcca ttgctgataa acctggtatc
     8641 gaacaggtgg cactgataga agcagctgtc agcaccaaag caacgttagc agaaatgttg
     8701 gcaagaatgg aaaatcgtgg cttagtcaga cgtgaacatg accccgctga taagcggcga
     8761 cgctttgtct ggttgactgc cgaaggcgaa aagatacttg ctgcggcgat acccattggt
     8821 gacagcgtgg atgaggaatt tttggggcgt ttgagtggag aagagcagga gctgtttatg
     8881 cagctggtgc gcaagatgat gaacgcatag ggggcaaatt tccggatgcg acgctatcgc
     8941 gtcttatcca gactacaaat acaccgactc accaaccgaa agaaaaaagg ccagcctcgc
     9001 ttgagactgg cctttctgac agatgcttac ttactcgcgg aacagcgctt cgatattcag
     9061 cccctgcgtt tgcaggattt cgcgcaaacg gcgcaggcct tcaacctgaa tctggcgaac
     9121 acgttcacgg gtgaggccaa tttcacgacc tacatcttcc agtgttgccg cttcgtaccc
     9181 cagcaaaccg aatcgacgtg ccagtacttc acgctgttta gcgttcagct cgaacagcca
     9241 tttgacgatg ctctgcttca tatcgtcatc ttgcgtggta tcttccggac cgttctcttt
     9301 ttcatcggcc aggatgtcca gcaacgcttt ttcggaatca ccacccagcg gggtgtctac
     9361 cgaggtaatg cgctcgttaa gacgaagcat acggctgacg tcatcaactg gcttatccag
     9421 ttgctctgcg atctcttccg cacttggttc atggtccagc ttatgggaca actcacgtgc
     9481 ggttcgcagg taaacgttca gctcctttac gatgtgaatc ggcaaacgaa tagtacgggt
     9541 ttggttcata atcgcccgtt caatcgtctg gcgaatccac caggttgcgt atgttgagaa
     9601 gcggaaacca cgttccgggt caaacttctc taccgcgcgg atcagcccca ggttgccctc
     9661 ttcgataagg tccagcaacg ccagaccacg attgccataa cggcgggcaa tttttaccac
     9721 cagacgcaag ttactctcga tcatccggcg gcgagaggcg acatctccac gcagtgcgcg
     9781 acgcgcaaaa taaacttctt cttcggccgt taacagtggt gaataaccaa tctcaccaag
     9841 gtaaagctga gtcgcgtcca acacacgctg tgtggctccc tgcgataaca gttcctcttc
     9901 ggccaaatcg ttatcactgg gttcctcttc tactaaagcc ttttcgtcaa aaacctcaac
     9961 tccgttctca tcaaattccg catcttcatt taaatcatga actttcagcg tattctgact
    10021 cataaggtgg ctcctacccg tgatcccttg acggaacatt caagcaaaag cctggttccg
    10081 ccgatttatc gctgcggcaa ataacgcagc gggtttacgg atttcccctt gtaacgaatt
    10141 tcaaaatgca agcgtgttga actggttccg gtgctaccca tggttgctat tttttgcccc
    10201 gccttcactt cttgttgttc ccggaccagc attgtgtcgt tatgggcgta ggcactcagg
    10261 taatcatcat tatgtttgat gataatcaga ttaccgtagc cgcgcagcgc gttaccagca
    10321 taaacaacgc ggccatctgc ggtcgcgata attgcctgtc ctttgctgcc tgcgatatca
    10381 atccccttgt tgcccccctc agaagcgcca aaggtttcga tcactttgcc ctcagtcggc
    10441 cagcgccagg tggagatagg cgtactggtt gatgtactgc tgacagttgg ctcggttgtg
    10501 cttgctgttg gtaccgttac aggcgctgtg accgtggtcg cagctggctt gttgttcggc
    10561 aacattttgt tagcactctg ttcacccgaa gattcagaat acgtaattgt cggttgcgac
    10621 gcaacagcaa cggtggaatt ttgtgcaggc ttgatcacaa ctccttgctc tgctgcgtcg
    10681 gcctgggtaa tggcatttcc gccagtgatt ggcgtaccgg aagcattacc cacctgcaag
    10741 gtctgaccaa cgttcagcgc gtatggtgcc tgaatattgt tgcgctgagc aaggtcacgg
    10801 aaatcgttgc cagtaatcca ggcgatatag aaaagtgtgt cgcctttttt cacggtatag
    10861 gtactgccgc tataactgcc tttcggaatg ttcccatact gacggttata gacgatgcgt
    10921 ccgttttcca tctgtaccgg ctgctgagct actggctgca ctggctggat ttgcggttgt
    10981 tgagtagcct gaatttgtgg ctgctgtacc ggctgaattt gcggttgctg ggctgtagac
    11041 gtcgtcccca ttttcggcgg cggcgtaatc aacataccag aattggtatt tgcaggcgca
    11101 ttgccattaa cggagctgac cggtgccggt ggatttgaag tgtcagaaca gcctgccagc
    11161 catagcgaaa ccagtgacaa agccgcaatg cggcgaacgg tgaattttgg gcttcccgcg
    11221 ctcatttatc ccccaggaaa aattggttaa taaccagtga cataattacc gtgcaaggca
    11281 ccctactgaa cactggaaaa gatgttcacg atacgctgac ctgcggcaaa ataaccagga
    11341 aaaatccagg tatttcctca cgttttaagc cagctcaccc ttcactaaag ggacaaagcg
    11401 cacggcctcc acggtatcga taataaattc gcctccccga cgacgcaccc gtttcaaata
    11461 ctggtgctcc tcccctacgg gtaagacgag aatcccgcct tcgtccagct gcgtcattag
    11521 cgcagttgga atttccggcg gtgccgccgt aacaatgata gcgtcaaacg gcgcgcgtgc
    11581 ctgccaacct tgccatccat cgccatgacg ggttgaaaca ttatgtaaat caagattttt
    11641 caggcggcga cgcgcctgcc actgcaaacc tttaatccgt tcgaccgagc aaacatgctg
    11701 gacaagatgc gccaggattg ccgtttgata tcccgaaccg gtgccaattt ccagcacccg
    11761 cgactgcggc gtcagctcga gtaattcggt cattcgcgcc accatatacg gctgcgaaat
    11821 tgtctgcccc tgacctatcg gcaaggcgat attgtcccag gctttttgtt caaacgcttc
    11881 atcaacgaat ttttcacgcg gcacggcagc aagtgcattc agcacctgct catcctgaat
    11941 accttgcgca cgtaattgat ccagaagtgc ttgtacgcgt ctgcttacca ttgcgtgcca
    12001 actcccacgc tgtttaacca gtctgaaacc acatcttgcg cgctatgcgc agttaaatcc
    12061 acatgcagcg gcgtgatgga gacatagccc tcatctaccg cagcaaaatc ggtccccgga
    12121 ccggcatcac atttaccgcc cggcgggcca atccagtaca gcgtattgcc gcgcggatct
    12181 tgctgcggga tcacctgatc tgccggatgt cgtgtaccgc agcgcgtcac gcgaatacct
    12241 ttgatttgat ccaagggtaa atccggaacg ttaatattaa gaatacgccc ggtgcgcaac
    12301 ggctctttac acagtgcgcg caaaattgaa caggttaccg ccgcggcagt gtcgtaatgt
    12361 ttatgcccgt caagcgagac ggcaagcgcc ggaaaaccta aatgacggcc ttccatcgcg
    12421 gcggctaccg taccggaata aataacatca tcccccagat tcggcccggc gttaattccg
    12481 gacacaacaa tgtccgggcg cggacgcatc agagcattca cgccaagata gacgcaatcg
    12541 gtcggggttc ccatttgcac cgcaatatca ccattttcaa aggtaaacgt gcgcagggag
    12601 gattccagtg tcagagaatt tgaagcgccg ctgcggttac gatcgggggc gaccacctga
    12661 acgtcagcaa actcacgcaa ggctttcgcc agcgtttgta taccgggtgc atgtaccccg
    12721 tcatcattac tcagcaatat gcgcataatc acctgttgtg ttgataagtt ccctgacaac
    12781 gctggttgca aaactacccg ccggaagcca gaaacggatc tctacggtga cgtcatccca
    12841 ccaattccag cttaattgtt gcggatacag cagcatcgct ctgcgcgcgg cttcaacttt
    12901 ttcgcgcacc agtaaagctt gtaattcagt ttctgcggcg acagctgctt gttcgaatgc
    12961 cagcgcttca cgctgagttc cccattcgcc actgcccggt aatgcggcgg ttatcatcag
    13021 ctctttatcg ttaacgcgac gctgtaattc cgccagttct tcggtggttg cgacaaacca
    13081 gctaccacgt ccggctaatt gtagcgcatc gccgtcaaca acttgattaa cgtctgcttt
    13141 tttgaggcgc tcagcaacaa tctgattaaa caacgcactg cgggctgccg acaaccaaaa
    13201 actccgttta ttgcgatcgc gcaccggagt attggtttgc gcccagcgct gcgccccctg
    13261 caagttgcta ccgccaatcc caaaacgttg ggcaccgaag tagttcggta caccttttac
    13321 gcaaatatcg atcagacgtt gttcaacgtc atcgcgattg ctcacttcgc gcagaaccag
    13381 ggtaaaggcg ttacctttca gcgcgcctaa acgcagcttg cgcttgtgcc gcgcatactc
    13441 cagcacctgg cagccttcca gttgaaaggc gctcagatcg ggcatttcct tgcctggcac
    13501 gcgagcgcat aaccactgtt ccgtaacagc atgtttgtct ttttgcccag cgaagctgac
    13561 ttcacgggca tgaattttca ggaatttcgc cagtgcatcc gccacaaaac gggtattgca
    13621 gccgtttttg aggattctaa ccagaatatg ctcaccttca ccatcaggct caaagcccaa
    13681 atcttccacc accacaaagt cttccggatt ggctttcagc agcccggtgc cttgcggttt
    13741 accgtggagg taagtgagat tatcaaactc aatcattttg ttgccttaat gagtagcgcc
    13801 accgcttcac aggcaatccc ttccccacgt ccggtaaatc caagtttttc cgtagtagtg
    13861 gctttcacgt taacatcatc catatggcag ccgagatctt cggcaataaa cacgcgcatt
    13921 tgtggaatgt gcggcaacat cttcggtgcc tgagcgatga tagtgacatc gacgttgccc
    13981 agggtataac ccttcgcctg aatacgacgc caggcttcgc gtagcagctc gcggctgtcg
    14041 gcacctttaa atgccggatc ggtatccggg aacagcttgc cgatatcccc cagcgccgcc
    14101 gcgccaagca atgcatcggt caacgcatgg agcgccacgt cgccatcaga atgcgccagc
    14161 aatccttttt cgtaaggaat gcgtacgcca ccaatgataa ttgggccttc accgccaaag
    14221 gcatgtacgt caaaaccgtg tccaattcgc attatgtatt ctcctgatgg atggttcggg
    14281 tgaggtaaaa ctcggccagt gccaaatctt ccgggcgcgt gactttaatg ttatccgcac
    14341 ggccttcgac caactgagga tggaatccgc aatattccag cgccgaggct tcgtcggtaa
    14401 tagtcgcgcc ttcatttaga gcgcgcgtca gacagtcatg taacagctca cgagggaaaa
    14461 attgcggcgt cagcgcgtgc cataagccgt tgcgatcaac ggtatgagca atggcatttt
    14521 tgcccggttc ggcacgtttc atcgtatcgc gcactggtgc ggctaggatc cctcccgtgc
    14581 ggctggtttc gctcaacgcc aacaatcgcg cgaggtcatc ctgatgcaga caaggacgag
    14641 cggcgtcatg caccaatacc cactgcgcgt cgccagcggc tttcaaacct gccagcacgg
    14701 aatcggcacg ctcatcaccg ccatctacaa cggtgatttg cggatgattc gccagaggaa
    14761 gttgtgcaaa acggctatcg ccaggactta tggcaatgac gacacgtttc acccggggat
    14821 gcgccagcag cgcatgcacc gagtgttcaa gaatggtttg attaccgatt gagagatatt
    14881 gcttaggaca ttccgtttgc attcgacggc caaatccggc cgccggaacc acggcgcaaa
    14941 catccaaatg agtggttgcc atgttaattc ccgggctgat ttatcgattg ttttgccccg
    15001 cagactgtgc gcgcttcgac gcgtcaggca ccagacgata aaaagtttcg cccggcctgg
    15061 tcatgctgag ttcattacgc gcacgctctt cgagcgcctc ctggccgcca ttgagatcgt
    15121 caatttcggc aaaaagttga tcgtttcgcg ctttaagttt cgcgtttgta gcttgctgtg
    15181 ccgccacatc atcattgacg cgggtatagt catgtatacc gttcttaccg aaccacagcg
    15241 aatactgtag ccagaccaga atagccagca acagcagcgt tagtttaccc atcctgcccc
    15301 ctgaaaaacg gcatcatcat cccatgcatc cgaagacgac tctacatcct ctgttgggga
    15361 taccgcgaca acgcgggcaa atgtaccaca tttgtccatt gttacgtata cccagggcgt
    15421 gcagaacata atctcattat tagttacggt ttgaattatg aacagaggag acaagaaagt
    15481 acaaattagc ccagtagcca cataaacagt gcgccaaaca taatgcctac tgtcaccagg
    15541 gtgaaaacaa tactgtagcg tagctttccg tccatcaatg aatgcagcgc aatccccacc
    15601 actaccgcga caggcatcag tgccagaaag aaaggccagg tgtagataaa gaagaacagc
    15661 gtgttagagc cataaatcaa catcggcatc gccagcgcaa ataaccagga gataaaaccg
    15721 accacggcac caggcagtga ccatgtggtt tcttcatcct cagtaaggct gtcgttattt
    15781 gttagtgtaa tgttatggct attacgcata tttgatcctg ttactttgac gaaccgggca
    15841 tggaaacccg gtggtgtctc aggatctgat aatatcgttc tgtctcaaca gatctaataa
    15901 ttgctgtacc aaatttgtta ctaattgttc accattgaga tgaatttctg ccgattcagg
    15961 cgcttcgtaa acggaatcta ttcccgtaaa gttgcgcagt tcaccggcac gcgctttctt
    16021 atataagcct ttgggatcgc gggcttcgca aatcgccagc ggcgtatcga caaacacttc
    16081 gataaagcgc ccttctccta cgcgttcgcg aaccatctgg cgttcggcgc ggtgtggcga
    16141 gataaatgcg gtcagcacca ccagtccggc ttcaaccatc aaattcgcca cttcaccgac
    16201 gcgacggata ttctctttac gatcggcatc gctaaaaccg agatcgctgc ataatccgtg
    16261 gcgaacattg tcgccatcca gcagatacgt actgacgccg agtttatgta acgcctcctc
    16321 cagcgccccg gcgaccgttg atttaccgga cccggagagg ccggtaaacc acagcactac
    16381 accacgatga ccgtggtgta gctcgcgttg ttgcacagtg accggatggc tatgccagac
    16441 gacgttttcg tcatgcagcg ccattattta tcccccagca aatcgcgcgc gccccagtgc
    16501 ggaaagtggc gacgaaccag agcattcaat tccagttcga atgcactgaa ttcagatggc
    16561 gcagcagttg cctggctaac tggctcgtgc accataccgg cacccacggt cacattgctc
    16621 aggcgatcga taaaaatcag cccacccgtc accggatttt gttgataacg atctaatacc
    16681 agcggctcgt caaaagtgag atccacgagg ccgatcccat tcagtggcag gttttcaact
    16741 tcacgctggg taaggttatt aatatcaacc tgatagcgaa tgccatcaac acgcgcgcgc
    16801 gtcttcttac cggcaatttt gatgtcgtaa ctctgccctg gagaaagcgg ctgttccgcc
    16861 atccatacca catccaccga cgcgctctgc accgccggta acgcttcgtc tgccgccagc
    16921 agcagatcgc cacggctgat gtcgatctca tccgtcagca ccagggtgat cgcttctccg
    16981 gcaaaggctt cttcgcgatc accatcaaaa gtcacgatcc gcgcgacgtt tgattccaca
    17041 ccagagggca gcacttttac acgttgcccg acttccacgc gaccggatgc cagcgttccg
    17101 gcgtagccac gaaaatcgag attcgggcga ttaacgtact gcaccgggaa gcgcattggc
    17161 tgagcatcca ccactcgctg gatctccacg gtttccagca cttcgagcag tgtcggaccg
    17221 ctgtaccacg gcatactttc actttgcgaa gccacgttgt cgccttccag cgcagagagc
    17281 ggcacaaagc gaatatccag attgccaggt aactgttcgg cgaaagtcag gtagtcttca
    17341 cgaatgcggg tgaatgtctc ttcactgtaa tccaccagat ccattttgtt gatcgctacg
    17401 accagatgtt tgatccccag cagcgtggag ataaaactgt gacgacgggt ttgatcgagc
    17461 acgcctttac gggcatcgat cagtaagatc gccagctcac acgttgaggc gccggtcgcc
    17521 atattgcggg tatactgctc gtgcccgggg gtgtcggcga taataaattt acgcttctcg
    17581 gtagagaaat agcggtaggc cacatcaata gtgatgccct gttcgcgctc agcttgcagg
    17641 ccgtccacca gcagagccag atccagtttt tcgccctggg tgccgtgacg cttactgtcg
    17701 ttatgcagcg atgagagctg atcttcgtag atttggcggg tatcgtgcag caggcgacca
    17761 atcagggtac ttttgccgtc atcgacgcta ccacaggtca gaaaacgcag caggctttta
    17821 tgttgttgcg caatcatcca ggcttcgacg ccgccttcat tggcgatttg ttgtgcaagt
    17881 gcggtgttca tcttaaaaat acccctgacg ttttttcagc tccatagacc ccgcctggtc
    17941 gcggtcaatc acgcggccct gacgttcact ggtggtggaa accagcatct cttcgatgat
    18001 ctccggcagt gtttgtgcat ttgactccac cgcaccggtc agcggccagc agcccagcgt
    18061 acggaaacgc accatccgtt ttttaatcac ttcgcccggt tgcaggtcga tacggttgtc
    18121 atcaatcatc atcaacatac cgtcgcgttc cagaaccgga cgttccgcag cgagatacag
    18181 cggcacaatg tcgatatttt ccagccagat gtattgccag atatcctgct cggtccagtt
    18241 agagagcggg aaaacgcgga tgctttcgcc tttgttaatc tgcccgttat agttgtgcca
    18301 cagctccggg cgctgatttt tcgggtccca gcgatggaag cgatcacgga aagagtagat
    18361 acgctcttta gcacgggatt tctcttcgtc gcggcgcgca ccaccgaagg cggcatcaaa
    18421 accgtattta ttcagcgcct gcttcaggcc ttcggtcttc ataatatcgg tatgtttcgc
    18481 gctgccgtgc acgaatggat taatccccat cgccacgcct tccgggtttt tatgcaccag
    18541 cagctcgcag ccgtaggctt tcgccgtacg atcgcggaac tcatacatct cgcggaattt
    18601 ccagccggta tcgacatgca gcaacgggaa aggcagcgta cctggataaa acgccttgcg
    18661 cgccaaatgc agcatgacgc tggaatcttt accgatagag tagagcatca ccggatttga
    18721 gaattctgcc gccacctcgc gaataatgtg gatgctttcc gcctccagtt gccgcaggtg
    18781 agtaagtcgt ttttgatcca taaccgttcc tttgcaatac cgctattttc ttgccatcag
    18841 atgtttcgac tatagggagc gtaagagaac gaatgaaatt accaattaga atgagtagtt
    18901 ccttaacgga ataacgattt ggcaaagcta atatcaaaaa gtgcttaagg caccggattt
    18961 cgggcgttta ggaagatttg aaattgtttt agcgcagcgg cagtttcata ctatggcggt
    19021 aaaaaaattt gcatggtatt taaggactca ctatgttttc cgcattgcgc caccgtaccg
    19081 ctgccctggc gctcggcgta tgctttattc tccccgtaca cgcctcgtca cctaaacctg
    19141 gcgattttgc taatacccag gcacgacata ttgctacttt ctttccggga cgcatgaccg
    19201 gaactccagc agaaatgtta tctgccgatt atattcgcca acagtttcag caaatgggtt
    19261 atcgcagtga tattcggaca tttaatagtc ggtatattta taccgcccgc gataatcgca
    19321 agaactggca taacgtgaca ggaagtacgg tgattgccgc tcatgaaggc aaagcgccgc
    19381 agcagatcat cattatggcg catctggata cttacgcccc gctgagcgat gctgacgccg
    19441 atgccaatct cggcgggctg acgttacaag gaatggatga taacgccgca ggtttaggtg
    19501 tcatgctgga attggcagaa cgcctgaaaa atacgcctac cgagtatggt attcgatttg
    19561 tggcgaccag cggcgaagag gaagggaaat taggcgctga gaatttactc aagcggatga
    19621 gtgacaccga aaagaaaaat acgctgctgg tgattaatct cgataactta attgttggcg
    19681 ataaattgta tttcaacagc ggtgtaaaaa cccctgaagc agtaaggaaa ttaacgcgcg
    19741 acagggcgct ggcaattgcg cgcagtcacg gaatagccgc aacgaccaat ccgggtttga
    19801 ataaaaatta tccgaaaggc actgggtgtt gtaatgacgc agaaatattc gacaaagcgg
    19861 gcattgctgt actttcggtg gaagcgacta actggaatct tgggaataag gatggttatc
    19921 agcaacgcgc aaaaacagcc gcattccctg cgggaaatag ctggcatgac gtaagactgg
    19981 ataatcaaca gcatattgat aaggcacttc ctggaagaat agaacgtcgc tgccgtgacg
    20041 ttatgcggat aatgctaccg ttggtgaagg agctggcgaa ggcgtcttga tgggtttgaa
    20101 aatgggagct gggagttcta ccgcaggggc ggggaattct aagtgatatc catcatcgca
    20161 tccagtgcgc ccggtttatc cccgctggcg cggggaacac aaaaccaaac ttctccataa
    20221 attccatagc cgcggtttat ccccgctggc gcggggaaca catatcctcc agaacacgcg
    20281 cctcatttcg actcggttta tccccgctgg cgcggggaac acactaagca tacataactg
    20341 tttttaaaca aatttattcc gcatcaacaa tctaccaact aaattaaaac atttccttat
    20401 ttttaaagaa cgtctaactt attgattatc aacaggaaga aaagaaacca aacgtaaccc
    20461 atccaaatcc accggaatac gtctgttttc gccccaggtc tgaaactcaa aaccagactc
    20521 agtatttgtc gcccaggcca tgaccacatt tccacaacct gccagttggg taatttgctg
    20581 ccagatcatc tcccgaatac gttttgatgt atcaccaaca tacacaccgg cacgcacttc
    20641 cagtagccag attgcgagcc gtccacgtaa gcgcggcggg acattttctg taacaaccac
    20701 gaccatgctc atcagccgcg tcctcgatga ccgctatcgc ccaacgtttc aggttcgggg
    20761 atggctggcg gtaacatatc cggcgcaggt tgtggcggtt caatttcacc tgcggcaagg
    20821 acttcctcaa ttaacggtat taatttgccc gttaacttag tgctacggaa aatatcacga
    20881 caggctaatc tgacttcttt atcaggttct gcgggttgcc tcgccgctat ttcaaatgcc
    20941 tttggtacaa ccgaaccaaa tttaatgata tcggctatgt cataaacaaa tgaaagcggt
    21001 ttgccactat ggataaaccc aatagcgggc gcatatcccg cggctaatac tgccgcttca
    21061 gatataccgt acagacatga tgtagcagca ctgatgcagc gattcacaac atcgcctttt
    21121 tcccagtctt taggatcgta tttgcgacca ttccatttca caccatattg tttcgccagt
    21181 aatgcatacg tctggcgaac gcgagatccc tcaattccac gtagctgctc cactgaacgg
    21241 cgagctggcg gtggctcacg aaaacgtaat tcatacattt tgcgcaccac cttcagacgt
    21301 agatcttccg ttaaagccag ctttgcctgg tagagtaact tatcagcacg cgccccaccg
    21361 ggttgtccgg aagagtagac gcgaacaccc gcttcaccga cccagaccag tagcgtcccc
    21421 accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg ttcccggttc gagcataatg
    21481 caggcgaccg atcccaccgg aatgtgcgtg cggatcccgg ttttatcgat cagcacgaaa
    21541 gcgccgtcca gtacgtcgat ttgcccgtac tggaggaaga tcatggaggt gcgatctttt
    21601 aatgggatcg ggctcagtgg tacaaacgtc acgcctctgc tccgggtttg atcagcatca
    21661 gaccacagcc gaacgcccgg cttttaccat acccctggct caggcgttgc acaaataacc
    21721 caggatccag gcgatgcatt tcccctgact taccgccgat tcgatatgcg caatacactc
    21781 ttgttcacta tgaagccaac ccacgctgac gctacgctca acgtctttgc gcgtcgctac
    21841 ccggtgtgag ttcacatcgg atttcgtgac atgcgtcagc caggggtaat catccttttc
    21901 caggaacgga gcttcttgca ggccctctgt accccgcgca aaggcagcga cgagtttgtc
    21961 gcgctgctgt tgagataacg tggcagaaag caaaataacg ctgtttccgc cacgcgcctg
    22021 ccgctcgatc aacccttcaa gaatgcacga catgtaagcg tcacaggcgt ggatctcatc
    22081 agccagcagg attttgttac tcagccccag aagccgcaga ttattatgtt tgaacggcat
    22141 caccgccatc atcgcctgat ccagcgtgcc gacgccaatt tcagccagca acgccttctt
    22201 gttactgtcg gcaaaccagg ccgcacatcc ctgactgaac gtttgttcat ccggttcttc
    22261 tgacccgact aaatcaccag accagagtga ttcattaaag cggtccatta atgtgcgggc
    22321 actatgtgcc agcaccaggc tggggcggga ttctggcgaa tagaaagcaa gccaggtttt
    22381 gaccagccga tcgtacatgg cattggccgt tgccattgtt ggcagaccaa aaaacagccc
    22441 ctgagctttc cccgcagcta tcaacctgtg cgccaggata agcgccgctt ctgttttacc
    22501 tgcgccagtc acatcttcca gaataaataa ctgcggtcct tgctggctga tatccagatc
    22561 cagcaccttt tgctgtaacg gtgtcggatt ctcaataaaa ggaaacagcg tattaattcc
    22621 gttgaaaggc gcgacttttg cttttagagg aaagacggtt aaggcgtttt gagcctgaat
    22681 taaagttttc tgccagtaac ctttaatatc cattgggtgt gcgacgcgtg gaaaaaatcg
    22741 cgttgacgaa cccgtccagt ctgcgagcac gacggttgca gagatatacc aggaaaggtg
    22801 ttttataagt tcaatgccct catcatcatc ccagaatgcg gggatctcta tgagcggaaa
    22861 cagtggtttg atttcaagga gaaaatctcg tgctgcagct ttgtcttcag gcagaaaatt
    22921 atccaattca tccatacgat cagggggccg accatgatgc cctgtcgtca cgggcatcca
    22981 catttctatc actcgcctaa gtttgcgcgg agagagcgaa gatgaaggaa gtaactcctg
    23041 acattcactt aaataatgat tccacaacca gtagcccagc gttgaatgag agattttttc
    23101 gtaatttttt ctggcacctt cctgaatctt gagttcaggg ggcaggtaaa gttgttgaaa
    23161 agagcgggca aattttccaa tatcgtgcca gcacagcaac caggcgaaaa attgagccgc
    23221 ctgttccttg tcagaaatcc ctaattgacg aaaataatca gccagcccga agcaatttct
    23281 tttaaccatt aaatagccca tcgcggctac atccagcgaa tgccagcaaa gaaggtgata
    23341 gtcgtcgccc ccgt
//
LOCUS       DAJSKJ010000053        22713 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000053 DAJSKJ010000000
VERSION     DAJSKJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.053"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..545
                     /locus_tag="ON345_001745"
     CDS             <1..545
                     /locus_tag="ON345_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR3071823.1"
                     /translation="PMQSAVNGQLGHWLQDSNACAVISFASGLVVMFFIIMARKETRQ
                     QFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASASALGVATFQTALISALLLSGLL
                     CDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQWHSTSFILLAILPFLAGLLAGW
                     QPAGNAKVAEATGSMLVSIT"
     gene            666..902
                     /locus_tag="ON345_001746"
     CDS             666..902
                     /locus_tag="ON345_001746"
                     /inference="COORDINATES: protein motif:HMM:NF016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071824.1"
                     /translation="MSIGLMAILVRGLGLLMLGVASTAGQLLGSVLIDVLIPSLGNSV
                     YLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            905..1939
                     /locus_tag="ON345_001747"
     CDS             905..1939
                     /locus_tag="ON345_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HCR3071825.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(1979..2271)
                     /locus_tag="ON345_001748"
                     /pseudo
     CDS             complement(1979..2271)
                     /locus_tag="ON345_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2351..3970)
                     /locus_tag="ON345_001749"
                     /pseudo
     CDS             complement(2351..3970)
                     /locus_tag="ON345_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4254..4664)
                     /locus_tag="ON345_001750"
     CDS             complement(4254..4664)
                     /locus_tag="ON345_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR3071826.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            4788..4913
                     /gene="cbtA"
                     /locus_tag="ON345_001751"
                     /pseudo
     CDS             4788..4913
                     /gene="cbtA"
                     /locus_tag="ON345_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            4910..5104
                     /locus_tag="ON345_001752"
     CDS             4910..5104
                     /locus_tag="ON345_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071827.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            5117..5230
                     /locus_tag="ON345_001753"
     CDS             5117..5230
                     /locus_tag="ON345_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCR3071828.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(5519..5665)
                     /locus_tag="ON345_001754"
                     /pseudo
     CDS             complement(5519..5665)
                     /locus_tag="ON345_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5719..5901
                     /locus_tag="ON345_001755"
     CDS             5719..5901
                     /locus_tag="ON345_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR3071829.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(6002..6331)
                     /locus_tag="ON345_001756"
     CDS             complement(6002..6331)
                     /locus_tag="ON345_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR3071830.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6503..7561)
                     /locus_tag="ON345_001757"
     CDS             complement(6503..7561)
                     /locus_tag="ON345_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3071831.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(7759..8232)
                     /gene="sbmC"
                     /locus_tag="ON345_001758"
     CDS             complement(7759..8232)
                     /gene="sbmC"
                     /locus_tag="ON345_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR3071832.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPSEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(8351..9517)
                     /gene="dacD"
                     /locus_tag="ON345_001759"
     CDS             complement(8351..9517)
                     /gene="dacD"
                     /locus_tag="ON345_001759"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCR3071833.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIQAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            9726..11153
                     /gene="sbcB"
                     /locus_tag="ON345_001760"
     CDS             9726..11153
                     /gene="sbcB"
                     /locus_tag="ON345_001760"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR3071834.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFSARIHLLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQNRNSRWDLLDVMRACYALRPDGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKAQQPKLFDYLYAYRGKNKLM
                     TLIDVPQMKPLVHVSGMFGAWRGNTSWIAPLAWHPDNRNAVIMVDLAGDIAPLLELDA
                     DTLRERLYTPKAELGDSSPVPIKLVHLNKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KLLRDNPQVREKVVAIFAEAEPFVASDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFADPRIEKLLFNFRARNFPGTLDENEQQRWLEHRRQIFTPEFLQAYAQQLEML
                     YGQYEGNNEKLSLLKALYQYVQEIV"
     gene            complement(11207..11431)
                     /locus_tag="ON345_001761"
     CDS             complement(11207..11431)
                     /locus_tag="ON345_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR3071835.1"
                     /translation="MVVKKLDVVTQVCPFPLIEAKAAMAELASGDQLVIEFDCTQATE
                     AIPQWAAEEGFTVTDYQQSGDAAWTITVQK"
     gene            complement(11444..12499)
                     /locus_tag="ON345_001762"
     CDS             complement(11444..12499)
                     /locus_tag="ON345_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015963872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCR3071836.1"
                     /translation="MLHMIISGMICGALLGFVMQRGRFCLTGGFRDMYITKNNRMFYA
                     LLIAICIQSIGVFALIQLGLVQYDAGAFPWLGTVVGGFVFGIGIVYAGGCATGTWYRA
                     GEGLIGSWIALVTYMVMSAIMRSPHAAGLNKFLGQHTTTHNGIAETLNISVWPLIVLL
                     VAVTLWFVRKELSKPKLKVASLPPRRTGLAHLLFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVGGDSKYLNWGVFLVLGIFIGSFIAAKASREFRVRA
                     ADASTTLRSAGGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWCASW
                     MLYVRPQRKAKLKTATA"
     gene            complement(12690..14048)
                     /gene="plaP"
                     /locus_tag="ON345_001763"
     CDS             complement(12690..14048)
                     /gene="plaP"
                     /locus_tag="ON345_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR3071837.1"
                     /translation="MSHNVTPNTSRVELRKTLTLIPVVMMGLAYMQPMTLFDTFGIVS
                     GMTDGHVPTAYAFALVAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWLFVIALVAFMTAFNLRSIKSVANFNTVIV
                     VFQVVLIAVILGMVIYGVAHGEGAGTLASNRPFWSGDAHVIPMISGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGLIFIFSTYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPTWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPICGALTVGALWVNLEESSMVLGLIWAGIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(14396..15217)
                     /locus_tag="ON345_001764"
     CDS             complement(14396..15217)
                     /locus_tag="ON345_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3071838.1"
                     /translation="MKKVAIVGLGWLGMPLALSLMARGWQVTGSKTTEDGVEAARMCG
                     IDSYPLRLDPQLVCEADDLDALMNVDALVITLPARRSGPGEDFYHQAVQEIVDSALAH
                     QIPRIIFTSSTSVYGKEEGLVKESTPREPVTASGRVLKELEDWLHQLPGTSVDILRLA
                     GLVGPDRHPGRFFAGKSAPDGQHGVNLVHLEDVVSAITLLLQAPKGGHIYNICAPLHP
                     ARGIFYPQMARELGLAVPSFSDSGNGKGKIIDGNLICRELGFEYQYPDPLRMPME"
     gene            15396..15446
                     /gene="hisL"
                     /locus_tag="ON345_001765"
     CDS             15396..15446
                     /gene="hisL"
                     /locus_tag="ON345_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR3071839.1"
                     /translation="MSRVQFKHHHHHHHPD"
     misc_feature    15422..15543
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            15578..16477
                     /gene="hisG"
                     /locus_tag="ON345_001766"
     CDS             15578..16477
                     /gene="hisG"
                     /locus_tag="ON345_001766"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR3071840.1"
                     /translation="MLDNSRLRIAIQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLIMDGVVDLGIIGENVLEEELLSRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPADEAWNGPAALDGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVMFRSKACLIQRDGEMSEAKQQLIDRLLTRIQ
                     GVIQARESKYIMMHAPTERLEEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKSLGASSILVLPIEKMME"
     gene            16483..17787
                     /gene="hisD"
                     /locus_tag="ON345_001767"
     CDS             16483..17787
                     /gene="hisD"
                     /locus_tag="ON345_001767"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR3071841.1"
                     /translation="MSFNTLIDWNRCSPEEQQALLMRPAISASDSITRTVAEILENVK
                     NNGDKALREYSAKFDKTEVGALRVTEEEIQQAAGRLSDELKQAMQAAVRNIDTFHTAQ
                     ILPPVDIETQPGVRCQQVTRPIASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKEVFNVGGAQAIAALALGTESIPKVDKIFGPGNAYVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAK
                     LAEGVAEAVERQLAELSRADTARQALSASRLIVANDLAQCVAISNAYGPEHLIIQTRN
                     ARELVDEITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTSTCSSLGLADFQKRMTV
                     QELSRDGFSAIAATIETLAAAELLTAHKNAVTLRVNALKEQA"
     gene            17784..18845
                     /gene="hisC"
                     /locus_tag="ON345_001768"
     CDS             17784..18845
                     /gene="hisC"
                     /locus_tag="ON345_001768"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR3071842.1"
                     /translation="MSIEQLARDNVRALTPYQSARRLGGKGDVWLNANEYPTAVPFEL
                     SQQTLNRYPECQPKAVIENYAAYAGVKPEQVLVSRGADEGIELLVRAFCEPGRDAVLF
                     CPPTYGMYSVSAETIGVECRTVPTKADWQLDLPAIADSLANVKVVYVCSPNNPTGQII
                     NPQDFRTLLEMTRDKALVVADEAYIEFCPQATLAVWLAEYPHLVILRTLSKAFALAGL
                     RCGFTLANEEVIALLMKVIAPYPLSTPVADIAAQALSAQGIAAMRERVAQILNERQYL
                     VDALRTIDCVEQVFDSETNYVLARIKASSSVFKSLWDQGIILRDQNKQPSLSGCLRIT
                     IGTRAESQRVIDALKAETV"
     gene            18842..19909
                     /gene="hisB"
                     /locus_tag="ON345_001769"
     CDS             18842..19909
                     /gene="hisB"
                     /locus_tag="ON345_001769"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR3071843.1"
                     /translation="MTQKFLFIDRDGTLISEPPSDYQVDRFDKLAFEPDAIPELLKLQ
                     KAGFKLVMITNQDGLGTDSFPQADFDGPHNLMMQIFTSQGVVFDDVLICPHLPADNCE
                     CRKPKTQMVTAWLADGVMDAANSYVIGDRATDVELANNMGITGLRYNRETLNWAAIGE
                     QLTRRDRYAHIERNTRETQIDVEVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMNI
                     TVKGDLYIDDHHTVEDTGLALGEALKVALGDKRGINRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYAMGVTLHLKTKGKNDHHRVESLFKVFG
                     RTLRQAIRVDGNALPSSKGVL"
     gene            19909..20499
                     /gene="hisH"
                     /locus_tag="ON345_001770"
     CDS             19909..20499
                     /gene="hisH"
                     /locus_tag="ON345_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR3071844.1"
                     /translation="MNVVVLDTGCANLNSVKAAIARHGYEPVVSRDPDVVLRADKLFL
                     PGVGTAQAAMDQIHERDLVELIKACTQPVLGICLGMQLLGRRSEETNGVDLLGIIDQD
                     VPKMTDHGLPLPHMGWNRVYPKAGDRLFRGIEDGAYFYFVHSYAMPVNEYTIAQCHYG
                     EAFTAAVQKDNFYGVQFHPERSGAAGAQLLKNFLEM"
     gene            20499..21236
                     /gene="hisA"
                     /locus_tag="ON345_001771"
     CDS             20499..21236
                     /gene="hisA"
                     /locus_tag="ON345_001771"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR3071845.1"
                     /translation="MIIPALDLINGTVVRLHQGDYGQQRDYGTDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAQRQIPLLKTLVAGVNVPVQVGGGVRTEDDVAALLDAGVARVVVG
                     STAVKSPEVVKGWFQRFGAEHLVLALDVRIDDNGAKQVAVSGWQENSGVTLEDLVETY
                     LPVGLQHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDLNDIAALRGTG
                     VRGVIVGRALLEGKFNVTEAIQCWQNG"
     gene            21218..21994
                     /gene="hisF"
                     /locus_tag="ON345_001772"
     CDS             21218..21994
                     /gene="hisF"
                     /locus_tag="ON345_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR3071846.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSIDDAARILSFGADKISI
                     NSPALADPELITRLADRFGVQCIVVGIDTWYDADTGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVRDVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRVC"
     gene            21988..22587
                     /locus_tag="ON345_001773"
     CDS             21988..22587
                     /locus_tag="ON345_001773"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR3071847.1"
                     /translation="MLTEQLDWQKTDGLMPAIIQHAVSGEVLMLGYMNEEALAKTQET
                     GKVTFFSRTKQRLWTKGETSGHFLNVVSIAPDCDNDTLLVLVNPIGPTCHKGTSSCFR
                     DTSHQWLFLYQLEQLLAERKNADPASSYTAKLYASGTKRIAQKVGEEGVETALAATVH
                     DKFELKNEASDLMYHLLVLLQDQELDLTAVIENLRERHK"
BASE COUNT         5528 a         6229 c         5840 g         5116 t
ORIGIN      
        1 cccctatgca atcagcggtg aatggtcaat taggtcactg gttacaggat agtaatgcat
       61 gcgcagttat ctcctttgcc agtggtttag tcgtgatgtt ttttattatt atggcgcgaa
      121 aagaaacccg gcaacaattt gcggcaattc cttctctgat taaaaaccga aaagttcctc
      181 tctggaactg gtttgccggt ctctgtggag cgatggtggt attttctgaa ggcgcatcag
      241 ccagcgcact cggggtcgca acgtttcaaa ccgcactgat ctccgcgcta ttgctgtctg
      301 gtttactttg cgatcgcttt ggtatcggtg tcgacgagaa aaaatacttt accccttatc
      361 gcatcatcgg tgcattattt gcggttattg ctacgatttt tgttgtgtca cctcagtggc
      421 attctacctc tttcatctta cttgccattc ttccgttctt agctggtttg cttgcaggat
      481 ggcagcccgc tggaaatgca aaagtagctg aagctacggg atccatgctg gtttcaatca
      541 cctagaattt catcgttgga ttttgtgttt taggcacagc gctagctatt cgtgtcgcat
      601 tagggcacgt cactgttcaa ttaccagatg tctgatggat gtatcttggc ggcccgcttg
      661 gactaatgtc tatcggttta atggccattt tggtcagagg tctggggtta ctgatgctgg
      721 gggttgcttc aacggcgggc cagttactgg gatcagtatt gattgacgta ttaattccat
      781 cgctgggcaa tagcgtttat ctggtcacga tcatcggaac actgtttgca ctggtcggtg
      841 caattgttac cactattccg gaatacagaa cctcgaaaac aatgaagaaa atggaggtat
      901 aatcatgaaa gattatctac aaactgtgac gggccccgtc gcccgtgaag atatggggct
      961 aacacttcct catgaacatc tgttcaacga tctttcctct gttgtggatg ctccttgcta
     1021 tcctttttca caacggcttg ttgataaaaa agtcacggca gaaatccagt gggcattaaa
     1081 acacgatcct tattgctgcg ctgataatat ggaccgcaaa cctattgaag atgtgatctt
     1141 cgaaattaat aactttatct cgctcggtgg tcgcaccatt gttgatgcca cagggtctga
     1201 gtctatcgga cgagatgcgc aggcattacg agaagtcgca ttaaaaacag gtctgaatat
     1261 tgttgcttcc tccggtccct acctggaaaa atttgaaagc cagagaattc ataaaacggt
     1321 tgatgaactg gcgacaacga tcgataaaga attgaatcaa gggattggcg atacggatat
     1381 tcgtgccgga atgatcggtg aaataggtgt ttcaccgaca tttaccgaag ccgagcataa
     1441 cagcttgcgg gctgcctcgc tggcacagat taacaatcct catgtggcga tgaatattca
     1501 catgccgggc tggcttcgtc gcggtgatga agtactcgac attgtgttag gtgaaatggg
     1561 cgtctcgcca aataaagtct ctctcgcaca ctcggatccg tcaggaaaag acgtggcgta
     1621 tcagcggaaa atgcttgata aaggtgtgtg gctggaattc gacatgattg gcctagacat
     1681 taccttcccg aaagagggaa tagcgccagg ggtgcaggag actgccgatg ccgtcgctca
     1741 tctcattgag ttgggatacg ccgatcagct tgttctcagc cacgatgtct tccttaaaca
     1801 aatgtgggct aaaaatggcg gtaatggctg gggatttgtt ccagatgttt ttctggccta
     1861 tctggcggag cgcggcgtcg ataaaacgat cctcaaaaaa ctctgtatcg ataatcccgg
     1921 acgattattg accgcataaa accattatcc cccggcacat tgcgccgggg gatgtcaatt
     1981 aataccccgc cgttaaatca tccaccaagc gcgagtcgga tgcgccgtac aactcaccat
     2041 ccgacccaac cataatgctt tgcgtgctgc ccatcgcctc attcagcgcc actttctgac
     2101 cttttgcatc cagcagcttg agcgtatccg gactaaaccc ttttcaacac gcagctcgtc
     2161 cggcaaccac tgatagtgga aacgaggcgc attggtcgct tcggcaacat tcattccaca
     2221 attgatgctg ttcaccacca tttgcagtac ggtagtgatg atccagctac cggtaacaaa
     2281 gtgagtacat aaaggccata ctgtcacgaa cagtgcaaaa gtggctcttc cgcaaccggg
     2341 gtgaagattt tcatgccagt ggactcatta ccctccgcct aagtagttca agtccggctc
     2401 gaccatacat ctggcgtatc agcaccttca ggcgatttac atgaccttcg acaacaccag
     2461 tactccagcg actgctgatt gcctcacata tagccgcagc atcagcttcc atccccgcag
     2521 ctacgcaccg aagttctgct gagccgcttt cgtggacccg agtgaaccag ctgcttagct
     2581 gtgatttatt ttaggttttc agaatacggt agaactcgag taccagttgc tgcgctattt
     2641 tcaactccgg ttctttttca cacatcagac taataaatcg gaaagcataa ttttcttcac
     2701 ccctgattat tctccagggc atcaaccagc ggcttacccg ggacactgaa ggaagtcttg
     2761 cggcagtagt aaccggtggg atccagcctt tacgccattt ggcaacagca tccctgacga
     2821 tggtttcact gcctgtaacc cccctggcca ccatttcccg ccatatccgg ccggagttat
     2881 aattaccaca ttctcgttgc tcttttaacc actccctcca tgggtccaga agccctcgct
     2941 ttggaggcct agttgacatt tcagggaatg ttcctgaacc cacccagcga ctgactgtca
     3001 ctcgcgacag gcctgtaata cgggatattt ccctgaaact acatcccttg ttatgcaggg
     3061 ccataacctc tgtccaacgc tgatgccgtt ttttgcggat gtgctgttta aggcgttcca
     3121 gacgacggag cgatgctaca ggcacagata tttctggctc aggtgatttc ttcagtgata
     3181 actctctgac aacaagacgt atcagaggca tatgtctgta catcattcgt tcaggctcat
     3241 caccaatatt ttttagcagg tgccatcgat cggccacctg tctggcctga ggtgcacctt
     3301 cacgtgctgc tgtcgcatag actccactgc gatcacgcga gacaacctgt atttccggat
     3361 attttctgaa ccaggtcgcc agcgtacgct gatcacggcc gggaagcagg acgaggggac
     3421 ggtgagtatc aagattgact attaacatac cgcagcagtg gccccgatgc cacgcccact
     3481 catcaatacc gacatgaggc gcgcctgact gtttcgtctc cggggtattg ataaccctgc
     3541 gaagaagagt atcagcactg caacggagac ccgcttccgt tgcagcccgt tttccagcct
     3601 cacctccagc tattaatccc agttgacgtt gtaaattttg taacgcctgt gaagactgct
     3661 ggtgtgaacc ggcgaaggga gcaagcgact cggcaaaaat tttacgtgaa caagccgggt
     3721 tacggcagta ccagtggcgg acggaaaata caagccatag cgtctgccct gagcagggta
     3781 gatgctgtat ccggcgccga cgacaactat gaacagagtc gctacgctta aagcattcag
     3841 gacattgtgc tgttttgcgt tttccgtgga gatggagggt tataccctca gagctaataa
     3901 tttgtcgact gcatcgccat ccgcaaggta actgtagaag tgtcttaaga gagtgcattg
     3961 cgttgcccat aacttaaaac agcttagatc cttgttcgcc agaaagtttc atccattttc
     4021 ttccacccag gttgcggaag aaccactttt catccgtcgt tgacagatgt gaagtatgcg
     4081 ggagaaaaag tgaatatcta actgtaataa ctcagaccgg tacgagtact cggaacgtct
     4141 acaatcattc agggcaaagt gctaaaattt ctctatcccg ggatgcccct accaacgcag
     4201 cactcccgga gatatcatta cagctctgca actgatttcc gcgactgaac cgctcagttc
     4261 ctatccttcc agttgccttc ctgcagcccc ccctccctgc tgtatacccc ggtaccacaa
     4321 cgcgggcagc acttttctcc ttcataatct tcgtcacaca tcacacaaaa ccactttctc
     4381 accttcaccg tataatccgg aaccgtcata ccctgcagat gcaaacgacg ggcctctttc
     4441 tctgccgggt caagccaggt gcatcgcagc aggattttct cagcgacagt catctggtca
     4501 tgttcaaacc acggtggttg tccgtcattg ccccgctgaa gtgttaacgg gcatccacag
     4561 gacacacagc acaaaacgtc ctggcggttg tgcttcgctg cttctttcgc cgtaacaaaa
     4621 tggcctttgc tgtcattcgc catataacag ttcagaattc gcatatttcc tcctgagttg
     4681 tccggggaat actgaatcct ccggtctggc agtgtgtgca gtaccgctcc ccataataat
     4741 cgtggtgaca ttgtctgcag tgccagctgg ctttacgcac cacgggtacc cgctctcagt
     4801 taataaacag catcgatatc ctccgggctc gcagggcgac cggcctgatg acccgcgaca
     4861 attacagaac ggtaaataac attaccctgg gtaagtatcc ggaggcgaaa tgatgacact
     4921 ggaagccgac agcgttaacg tacaggcgct ggatatgggg cacattgtcg ttgacattga
     4981 tggtgttaat atcactgaac tgattaataa ggccgctgaa aacggttatt cactccgcgt
     5041 ggtggatgac cgtgactcta ccgaaacacc ggcaacttat gccagccctc accagttgct
     5101 gtaagacaat gcagtgatgc agggtatagt gccattaacc acagaaacag cactgttgtc
     5161 ctgtattaag caggcaagag aagatgtcct taccctgcgc catctgcagc ttctgcacca
     5221 gaaccggtga atctcactca acaggcttca tttcccctga cgccagcctg aatacagctg
     5281 gcgttttcat ttataaacag aaaggaaaac cgataataat ggaatggacg ccccgaccat
     5341 gaagcaacgg taggatatta ctgataaacc ttccagtgga ggtgcgttat ggccgtttta
     5401 acgattagca ttgacctgac aaaaaatgaa ttccaaatcc acggtctggg taggaacaga
     5461 aaaatctaag ctcagaaaac ggattaaagt aagcgtcaac ggagcaccgt attgacgctt
     5521 atttattggt gagaactacg ttccatggca ggagttcgtc aacacggttg gagggccatt
     5581 ctggcagtat gctcagaata tggcgcagat acgcttccgg atcgataccg ttcagacggc
     5641 aggtgcctgc ttttccggca acaagcagga atctcagcgc tgaagcagga attataggta
     5701 aaaacgccct accgggccat gaatcacccg gtcattggag tagtaaccaa agcagattta
     5761 gccagcatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
     5821 gtgttagtaa ccagtgcggt taataacaat ggagttacag aactttttgc cttgctgcat
     5881 acagaagaag gctgttgcta atttcgatag gttctcagat gagaatgatg acggtgatac
     5941 acagataaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
     6001 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
     6061 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
     6121 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatcccg ggcttgatgt
     6181 aatcgctcag gttgtccagc aacaggacgc gcttctggtt gtcacggatc tttttctcaa
     6241 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
     6301 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
     6361 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
     6421 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcggcagca
     6481 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
     6541 actcaagctg atgtgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
     6601 caaccttcag gtcatggtgg ttattcagca actgacgcag ctcttccaca gcatcgttca
     6661 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaggc
     6721 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
     6781 cgaaatggct ggggcgcgtg gcccagtatg cattgatttg caactccagc atacaaacca
     6841 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaattcga gtttctttgc
     6901 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
     6961 tttccagacg tgggcgttcg agtaagtttg gtgagaatgc agattgataa acccgattat
     7021 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
     7081 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
     7141 ccgtatcaat ttcacctgtc ggggaaccca caacaattgc cagcgtcacc ccaatcaata
     7201 atccttgata cggtttcttg cccagtgcca gccaaccgca aagaaacata gccgccgcgc
     7261 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
     7321 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcgcg aggaacaacg ttaccccaga
     7381 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tcagggatag
     7441 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
     7501 caatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
     7561 cgcaaaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
     7621 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
     7681 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
     7741 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
     7801 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
     7861 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
     7921 atcaccgact acacgagcca ccgccaccgc atactgacca ccggaaattt ctgtcagaat
     7981 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
     8041 gcgtaatttt tcggagggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
     8101 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
     8161 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcttaat
     8221 ctcgtagttc atgacgcctc cattaacact gtttttatat acagtatagt tgcaaaatta
     8281 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
     8341 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
     8401 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
     8461 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
     8521 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
     8581 gttccgttcc caggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
     8641 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
     8701 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
     8761 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
     8821 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
     8881 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
     8941 ggatgatagc gcgagaaagc acagctaaat cataagccga gctatgctgg ccaggtgcat
     9001 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
     9061 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
     9121 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
     9181 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
     9241 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
     9301 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
     9361 ccgcggtgag gatctgtccg gtggtgtaat ccatcagtac ccaggacccg gcttgaatct
     9421 gtggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
     9481 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
     9541 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
     9601 gactaccctc gcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
     9661 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
     9721 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
     9781 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgatagcg
     9841 aattcaatgt catcggagaa cccgaagtct tttactgcaa gcccgctgat gactatttac
     9901 cccagccagg agcagtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
     9961 aaaacgaagc cgcgttttcc gcccgtattc acttgctttt taccgtaccg aagacctgta
    10021 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacatt ttttatcgta
    10081 atttctacga tccttacgcc tggagctggc aaaaccgcaa ttcgcgctgg gatctgcttg
    10141 acgtgatgcg cgcctgctat gccctgcgcc cggacggcat caactggccg gaaaacgacg
    10201 acggtctgcc gagcttccgc ctggaacatc tcaccaaggc caacggtatt gaacacagta
    10261 acgcccatga tgcgatggcc gacgtttacg cgaccatcgc catggcgaag ctggtgaagg
    10321 cccaacagcc aaagctgttt gattatctgt atgcctaccg gggcaaaaac aagctgatga
    10381 cgctgattga cgtcccgcag atgaagccac tggtgcatgt ctcgggaatg tttggcgcct
    10441 ggcgcggaaa caccagctgg attgcgccgc tggcctggca tccggataac cgtaatgcgg
    10501 tgatcatggt cgacctggcg ggcgacatcg ccccgctgct cgagctggat gccgatacgc
    10561 tgcgcgagcg cctgtatacg ccgaaggccg agctcggcga cagttcgccg gtgccgatta
    10621 agctggtgca tctgaataag tgcccggtgc tggcgcaggc caataccctg cgcccagaag
    10681 acgctgaccg tttaggcatt aaccgacagc actgcctgga taacctgaaa ctgctgcgcg
    10741 ataacccgca ggtgcgcgag aaggtggtgg cgatttttgc tgaagccgag ccgtttgtcg
    10801 catcggacaa cgtcgacgcc cagctttata acggtttctt tagcgacgcc gaccgcgcgg
    10861 cgatgaaaat cgtgctggag accgagccgc gcaatctgcc ggcgctggac attaccttcg
    10921 ccgacccgcg tattgagaag ctgctgttta acttccgcgc gcgcaacttc cccggcacgc
    10981 tggatgaaaa cgagcaacag cgctggctgg aacaccgtcg gcagatcttc accccggaat
    11041 ttttacaggc ctacgcccag caactggaga tgttgtacgg tcagtatgag gggaataacg
    11101 agaagctatc gctgctgaag gcgctgtatc agtacgtgca ggagattgtt taattcaacg
    11161 cagcgacgcc ggaaaatctt cccggcgtcg cttgatgcct ggggcattat ttctgtacgg
    11221 tgatggtcca cgccgcatcg ccagactgct gatagtccgt gaccgtaaag ccctcttccg
    11281 ccgcccactg cgggatggct tccgtcgcct gagtacagtc gaactcaatc accagctggt
    11341 cgccgctggc cagttccgcc atcgccgcct tcgcttcaat taacgggaac gggcaaacct
    11401 gcgtgactac gtccagtttc ttcacaacca ttttttctac tccttaagcc gttgcggttt
    11461 tcagttttgc tttccgctgc gggcgaacat acaacatcca cgacgcgcac cacacgccga
    11521 gaatcataaa taccaggcca atccagccct gccaggtcat catcgccgtc atcaccaaac
    11581 cgttgccgat cgagcagccg ccggcaatgc tggcaccgaa gcccatcagt acgccgccac
    11641 ccgcgctacg cagcgtggtg ctggcatcgg cagcgcggac gcggaattca cggctggctt
    11701 tcgcggcaat aaacgaaccg ataaagattc ccagcaccag gaagacgccc cagttgaggt
    11761 acttgctgtc accccccacc aggaactgca ggatattggc ggtcggcgag gtaatgccaa
    11821 ggccaaacat acgtccggtg gcctcactca gcggccaggc cagcagggcg ataaggccaa
    11881 tcagcacggc ggtcacaaac ggatgccaac gcttttcaaa cagcagatgc gccaggccag
    11941 tgcggcgcgg cggcagagaa gctactttga gctttggttt gctcagctct ttgcgaacaa
    12001 accacagggt caccgccacc agcagaacga taagtggcca gacggagata ttcagggttt
    12061 cggcaatgcc gttgtgagtg gtcgtgtgtt gaccgaggaa tttgttaagc ccagccgcgt
    12121 gcggtgaacg catgatcgcg ctcatcacca tataggtgac cagtgcaatc cagctgccaa
    12181 tcagcccttc cccggcacga taccaggtgc cggtggcgca gcctcccgca taaacgatac
    12241 cgataccaaa aacaaatcca cccaccacgg tgccaagcca cgggaacgcg cctgcatcgt
    12301 actgcaccag gccaagctgg atcagggcga acacgccgat actctggata cagatagcaa
    12361 tcaacagtgc gtaaaacatg cggttatttt tggtgatata catatcgcga aagccacccg
    12421 tcaggcagaa acgaccgcgc tgcataacga atcccagtaa agccccgcaa atcatgccac
    12481 ttattatcat gtgcaacata agaatcaatt aactcaatat tatcagtctt aaaattattc
    12541 ctgaccccgc cggacggctc caataaccaa aagctctata ttattcccga cggttattaa
    12601 tattgatttt ttaatcaccc cacaaactta agacaaaaaa aaccggaggc ccagacctcc
    12661 ggttttgcaa ccatcgtttt taaattcagt tatgcaacat cttcgtactg cggaaccggg
    12721 ttgcggaagc ttttggtcac acaggccagg tagatcagac cgataccggc ccagatcagg
    12781 cccagaacca tagagctctc ttccaggttc acccacagtg cgccaacggt cagcgcgccg
    12841 cagattggca ggaacaggta ctggaagtga tctttcagcg tcttattacg cttctcacgg
    12901 atccagaact gagagataac ggacaggttc acgaaggtga acgccaccag cgcaccgaag
    12961 ttaatcagcg ccgttgccat caccaggtcg aagttaatcg ccagcagcgc aatcgcgcca
    13021 accaggatga tgttcatcgc cggggtgcgc caggttggat gcacgtagcc gaagaagctt
    13081 ttcgggaaca cgccatcacg gcccatcacg tacatcagac gcgccacacc cgcatgcgcc
    13141 gccataccgg aagccagcac ggtaatggtg gagaagatca gcgcgccaac ctggaaggct
    13201 ttacccgcca cgtacagcat gatttcaggc tgtgaggcgt ccggatcttt gaagcgagag
    13261 atatccggga agtagagctg caggaaatag gtggagaaga taaagatcag gccaccaatc
    13321 agcgccgtca ggaagatagc acgcggaatc acacgctcag cgtctttggt ttcttcagac
    13381 aggttgctga taccgtcaaa accggtaaag gagaagcaca gaatcgtggc cccggaaatc
    13441 atagggatca cgtgcgcatc acccgaccag aacggacggt tgcttgccag cgtaccggca
    13501 ccttcgccgt gcgccacgcc ataaatcacc atacccagga tcacggcaat cagtaccacc
    13561 tggaacacca cgatcacggt gttaaagttg gcgacagatt tgatgctgcg caggttaaag
    13621 gcggtcataa aggcaaccag tgcaatcacg aataaccatg acggtatcga cggcaccagc
    13681 gcttcgaaat agatttttgc cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc
    13741 agcgaagacc aacccaccat aaagccaacg gtcgggctga tggatttctg ggcataagtg
    13801 taggcagagc ccgccgaagg ataacggcgc accagcttac cgtagctcag cgcggtgaac
    13861 agaatcgcca ccagagcgaa ggcataagct gttggcacgt gaccatcggt catgccagat
    13921 acgataccga acgtatcaaa cagcgtcatc ggctgcatat aggccaggcc catcataaca
    13981 accggaataa gcgtaagcgt tttacgtaat tccacgcgag aggtgtttgg agtaacgtta
    14041 tgcgacacgg tcagtctcca ttacggcgat ggccggcgca aaattgaaaa attgccccat
    14101 tttttttctg tttcctcagc gacaacgact gtcgtttttt aagtaagtat ctatccggta
    14161 cgaagcccgg cctctttaat ttgatggttg ttttgatgta aaaaaaataa ccgacgcggc
    14221 aagcacgtcg gttaatctca attttttaca ttcgacatat tttgccccaa acggtagcaa
    14281 aaagacaagc cgctgagagc ataatcagca cgccttctcg tctaaacgac tgataattca
    14341 gcaactcccg aatcataaat ctcatcaata gcactatttg ctaaaacaga ggcgattatt
    14401 ccatcggcat acgtaacggg tcaggatact gatactcaaa gccgagctca cggcaaatga
    14461 gattaccgtc gatgattttg ccttttccat taccgctgtc gctaaagctc ggaaccgcta
    14521 accccagctc gcgcgccatt tgcggataga aaataccgcg agcgggatgt aacggcgcac
    14581 aaatgttata aatgtgtcca cccttcggcg cctgtaataa aagcgtaata gcagaaacaa
    14641 catcttccag atggacgaga ttcacgccgt gctgaccatc cggcgcggat ttacctgcga
    14701 agaaacgtcc cggatgccga tccggcccca ccagccccgc cagacgcaga atatccaccg
    14761 acgtccccgg caattgatga agccagtctt ccagctcttt taataccctg ccgctagcgg
    14821 taaccggttc acgcggcgtg ctctctttta ccagcccttc ttctttaccg tagaccgaag
    14881 tggagctggt aaagataatg cgcggaatct ggtgcgctag cgcggagtct acgatctcct
    14941 gcaccgcctg atgatagaaa tcttcgcccg ggccgctgcg gcgcgcgggg agcgtaatga
    15001 ccagcgcgtc aacattcatt aaagcatcaa ggtcgtcagc ctcacagacc agctgcggat
    15061 cgagccgcaa cggatagctg tcaatcccgc acatccgtgc ggcttccacg ccatcctcag
    15121 tcgttttact gcccgttacc tgccagccgc gcgccatcaa cgacagcgcc agcggcatac
    15181 ccagccaccc taaaccgaca attgccacct tcttcattgc gctatctcct ggttcatcct
    15241 gcactcctct cataaggcta cgccacagcg agaaaactga caatttatag tggtaacatg
    15301 aattgattta atgaatgaaa aaagcacttg cgctcaacga caaaagtggt ttaggttaaa
    15361 agacatcaag tgaatagtca ttcaacgaga aatttatgag ccgcgttcaa tttaaacacc
    15421 accatcatca ccatcatcct gactagtctt tcaggcgatt tgtgctggga gacattcaga
    15481 tcttccagtg gtgcatgaac gcaagagagc ccccggaaga tcatcttccg ggggcttttt
    15541 tttgaaccaa attcagcagg gtaatgagga atacagaatg ttagataaca gccgtttacg
    15601 catagctatt cagaaatcag gccgcctgag cgatgattca cgcgaattac tggcccgttg
    15661 tggtatcaaa atcaatctcc acacccagcg cctgatcgcg atggcggaga acatgccgat
    15721 cgatatcctg cgcgttcgcg atgacgatat tccggggctg attatggatg gcgtggtcga
    15781 tctcggcatt atcggtgaaa acgtgctgga agaagagctg ctgagccgcc gcgctcaagg
    15841 cgaagacccg cgctacttta ccctgcgtcg tctggacttc ggcggctgcc gtctgtcgct
    15901 ggcgaccccg gcggatgaag cctggaacgg cccggctgcg ctcgacggca aacgtattgc
    15961 cacctcttac ccgcacctgc tcaaacgtta tctcgaccag aaaggcatca gctttaaatc
    16021 ctgcctgctg aacggctctg ttgaagtggc cccgcgtgcc ggtctggccg acgccatctg
    16081 cgacctggta tcaaccggcg cgacgctgga agccaacggc ctacgtgaag tagaagtgat
    16141 gttccgctct aaagcctgcc tgattcagcg cgacggcgaa atgagtgaag cgaaacagca
    16201 gctgatcgac cgcctgttaa cccgtattca gggcgtgatt caggcgcgtg aatcgaaata
    16261 catcatgatg cacgcgccga ccgaacgtct ggaagaagtg atcgccctgc tgccaggtgc
    16321 cgagcgccca accattctgc cgttagcagg cgaccagcag cgcgtggcga tgcacatggt
    16381 cagcagcgaa accctgttct gggaaaccat ggaaaaactg aagtccctgg gtgccagctc
    16441 gattctggta ctgccgattg agaagatgat ggagtaagcg ccatgagctt caataccctg
    16501 attgactgga accgctgtag ccccgaagaa cagcaggcgc tgttaatgcg cccggccatt
    16561 tccgcttccg acagcatcac ccgtacggtg gccgaaattc tggaaaacgt taaaaataac
    16621 ggcgataaag cgctgcgtga atacagcgcg aagtttgata aaaccgaggt tggcgcgctg
    16681 cgcgtgactg aggaagaaat tcagcaggcc gcaggccgtc tgagcgacga actgaagcag
    16741 gcgatgcagg ctgcggtgcg caacatcgat accttccaca ccgcgcagat tctgccgccg
    16801 gtggatatcg aaactcaacc gggcgtacgc tgccagcagg tcacccgccc gatcgcctcc
    16861 gtggggcttt atattccggg tggttcagcg ccgctgttct ccaccgtatt aatgctggcc
    16921 accccggcac gcatcgccgg ctgtaaaaaa gtggtgctgt gctcgccgcc gccgattgcc
    16981 gatgagattc tgtatgcggc gcagctgtgc ggcgtcaaag aggtatttaa cgtcggcgga
    17041 gcgcaggcca ttgccgcgct ggcgctgggc accgagtcta ttccgaaggt ggataaaatc
    17101 ttcgggccag gcaatgcgta cgtcaccgaa gcaaaacgcc aggtcagcca gcgtctcgac
    17161 ggcgcggcca tcgacatgcc agcaggcccg tctgaagtac tggtcatcgc cgatagcggc
    17221 gccacgccgg actttgtcgc ctccgacctg ctctctcagg ccgagcacgg cccggactca
    17281 caggtgattc tgctgacgcc agacgcgaaa cttgccgaag gcgtggccga ggccgttgaa
    17341 cgccagcttg ccgagctgtc ccgcgccgat accgcgcgtc aggccctctc tgccagccgc
    17401 ctgattgtgg ccaacgatct ggcgcagtgc gtggcgatct ccaacgccta cggcccggaa
    17461 cacctgatta ttcagacccg caacgcgcgt gaactggttg atgagatcac cagtgccggt
    17521 tcggtattcc ttggcgactg gtcgccggaa tcggctggcg actatgcctc cggtaccaac
    17581 cacgtgctgc cgacctacgg ttacacatcc acctgttcca gcctgggact ggcggatttc
    17641 cagaaacgga tgaccgttca ggaattatcc cgtgacgggt tctcggccat tgccgccacc
    17701 attgaaacgc tggccgccgc cgagctgctg accgcccata aaaatgccgt taccctgcgc
    17761 gtgaacgccc tgaaggagca agcatgagca ttgaacagtt agcccgcgac aacgttcgcg
    17821 ccctgacccc gtatcagtcg gcgcgccgcc ttggcggtaa aggcgacgtc tggctgaacg
    17881 ccaacgaata tccgacggcg gtgccgttcg aactcagcca gcaaacgctg aaccgctacc
    17941 cggaatgcca gccaaaagcg gtgattgaaa actacgctgc ctatgcgggc gtcaagccgg
    18001 agcaggtgct ggttagccgc ggcgcggatg aaggcattga actgctggta cgcgcgtttt
    18061 gcgaaccggg ccgcgatgcg gtgctgttct gcccgccgac ctacggcatg tacagcgtca
    18121 gcgctgagac catcggcgtc gaatgccgca ccgtgccgac caaagccgac tggcagctgg
    18181 atctcccggc cattgccgac agtctggcca acgtgaaagt ggtttacgta tgcagcccga
    18241 acaacccaac cgggcagatt atcaacccgc aggatttccg tacgctgctg gagatgaccc
    18301 gtgacaaagc gctggttgtc gccgatgagg cctatattga gttctgcccg caggccacgc
    18361 tggccgtctg gctggccgag tacccgcatc tggtaattct gcgcacgctg tccaaagcct
    18421 ttgcgctagc ggggctgcgc tgcggcttta cgctggcgaa tgaagaggtg atcgcgctat
    18481 taatgaaggt gattgcgccg tacccgctct cgaccccggt tgccgatatt gcggcccagg
    18541 cgcttagcgc gcaaggaatc gccgccatgc gcgaacgcgt cgcgcaaatc ctcaatgaac
    18601 gtcagtacct ggtggatgcg ctgcgcacca tcgactgcgt cgagcaggta tttgattccg
    18661 agaccaacta cgtactggct cgtattaaag cctcaagcag cgtatttaaa tctttatggg
    18721 atcaaggcat tatcttacgt gaccagaata aacaaccttc tttaagcggc tgtttgcgca
    18781 tcactatcgg cactcgtgca gagagtcagc gcgtgattga cgctttgaaa gcggagaccg
    18841 tatgacccag aagtttctct ttatcgatcg cgatggcacc ctgatttccg aaccgccgag
    18901 cgactatcag gtagaccgtt tcgataaact ggccttcgag ccggacgcca ttcccgaact
    18961 gctgaagctg caaaaagcgg gttttaagct ggtgatgatc accaaccagg acgggctggg
    19021 caccgacagc ttcccgcagg cggatttcga cggcccgcac aacctgatga tgcagatctt
    19081 tacctcgcag ggcgtggtgt ttgacgatgt gctcatttgc ccgcacctgc ccgccgacaa
    19141 ctgcgaatgc cgcaagccga aaacgcagat ggtcaccgcc tggctggcgg acggcgtgat
    19201 ggacgctgcc aacagctacg tgattggcga ccgcgccacc gacgtcgaac tggccaacaa
    19261 tatgggcatc accggcctgc gctacaaccg cgaaacgctt aactgggcgg cgattggcga
    19321 gcagttgacc cgccgcgacc gctacgccca cattgagcgc aacacccgcg aaacgcagat
    19381 tgatgtcgaa gtgtggctgg atcgcgaagg cggcagcaaa attaacaccg gcgttggttt
    19441 cttcgaccac atgctggacc agatcgccac ccacggcggt ttccgtatga acattaccgt
    19501 gaaaggcgac ctctatattg acgatcacca cacggtggaa gataccggcc tggcgctggg
    19561 cgaagcgctg aaagtcgcgc tgggcgataa gcgcggcatt aaccgtttcg gcttcgtgct
    19621 gccgatggac gaatgcctgg cccgctgtgc gctggatatt tccggccgtc cgcacctcga
    19681 atacaaagcc gagtttacct atcagcgcgt aggcgacctg agcaccgaga tgatcgagca
    19741 cttcttccgc tcgctctcgt atgccatggg cgtcaccctg cacctgaaaa ccaaaggcaa
    19801 aaacgatcac caccgtgtgg agagcctgtt taaagtcttt ggccgcacgc tgcgccaggc
    19861 gatccgcgtg gacggcaatg cccttccctc ctcaaaagga gtgctgtgat gaatgtggtt
    19921 gtccttgata ccggctgcgc caacctcaat tcggtgaaag cggcgattgc ccgtcacggc
    19981 tacgagccgg tggtcagccg tgacccggat gtggtgctgc gcgctgacaa actgttcctg
    20041 ccgggcgtgg gcactgcgca ggcggcgatg gatcagattc acgagcgcga cctggtcgag
    20101 ctgattaaag cctgcaccca gccggtgctg ggcatctgcc tcggcatgca gctgctggga
    20161 cgccgcagtg aagaaacaaa cggcgtcgac ctgctgggaa ttattgacca ggacgtgccg
    20221 aaaatgactg accacggctt gccgctgcca cacatgggct ggaaccgcgt ctatccgaag
    20281 gcgggcgacc gtctgttccg cggcattgag gacggcgcgt atttttactt cgttcatagc
    20341 tacgcgatgc cggtcaatga atacaccatc gcgcaatgcc actacggtga agcgtttacc
    20401 gccgcagtac agaaagataa cttttacggc gtgcagtttc accccgaacg ctccggcgcg
    20461 gcgggcgcac agctgctgaa aaacttcctg gagatgtaat gatcattccg gctttagatt
    20521 tgattaatgg caccgtggta cgcctgcatc agggtgatta cggacagcag cgcgactacg
    20581 gcaccgatcc gcttccccgt ttacaggact acgcggctca gggtgccgaa gtgctgcacc
    20641 tggttgattt aaccggtgcg aaagacccgg cacagcgcca gattccgctg ctgaaaaccc
    20701 tggttgccgg cgtgaacgtg ccggtacagg ttggcggcgg cgtgcgcacc gaagatgacg
    20761 tcgcggcact gctggacgcg ggtgtggcgc gcgtggtggt cggctcaacg gcggtgaaat
    20821 cgccggaagt ggtgaaaggc tggttccagc gctttggcgc tgagcacctg gtgctggcgc
    20881 tggacgtgcg catcgatgat aacggcgcca aacaggtggc ggtgagcggc tggcaggaga
    20941 attccggcgt cacgctggaa gatctggttg agacctatct gcccgtgggg cttcagcacg
    21001 tgctgtgcac cgatatttcc cgcgacggca cgctggcagg ctccaacgtt tcgctgtatg
    21061 aagaggtctg cgcccgctat ccgcaggtgg cgttccagtc ctccggcggc attggcgatc
    21121 ttaacgatat cgccgcccta cgcggcaccg gcgtgcgcgg cgtgattgtc ggccgcgcgc
    21181 tgctggaagg taaattcaac gtaacggagg ccatccaatg ctggcaaaac ggataattcc
    21241 ttgtcttgat gtgcgtgatg gtcaggttgt taagggcgtg cagttccgca atcacgagat
    21301 catcggcgat atcgtcccgc tggccaaacg ctacgccgac gaaggcgcgg atgaactggt
    21361 gttctacgat atcaccgctt ccagcgatgg ccgcgtggtc gataaaagct gggtttcccg
    21421 tgttgccgaa gtgattgata ttccgttctg cgtagcgggc ggcattaagt ccattgacga
    21481 tgcggcgcgc attctctcct tcggcgccga taaaatttcc atcaactccc cggcgttggc
    21541 cgacccggag ctgatcaccc gtctggcgga ccgcttcggc gtgcagtgca tcgtggtggg
    21601 tattgatacc tggtatgacg ctgacaccgg caaatatcac gtcaatcaat acaccggcga
    21661 tgaaagccgc acccgcgtga cccaatggga aacgctggac tgggtgcagg aagtgcagaa
    21721 acgcggcgcg ggcgagatcg tcctcaatat gatgaaccag gacggcgtgc gtaacggcta
    21781 cgacctggag cagttgaaaa aagtgcgcga cgtttgccgc gtaccgctga tcgcctctgg
    21841 tggtgcaggg accatggaac acttcctcga agcgttccgt gatgccgatg ttgacggcgc
    21901 gctggcagcc tcggtattcc acaaacagat tattaatatt ggtgaactaa aagcgtacct
    21961 ggcaacacag ggcgtggaga ttcgggtatg ttaactgaac aactggactg gcaaaaaacc
    22021 gacggactga tgcccgccat tatccaacac gcggtatccg gtgaagtgct gatgctgggg
    22081 tatatgaacg aggaagcgct ggccaaaacg caggaaacgg gcaaagtgac ctttttctcg
    22141 cgcaccaaac agcgcctgtg gaccaagggt gaaacctccg gccacttcct caatgtggta
    22201 agcatcgcgc ccgactgcga caacgacact ctgcttgtgc tggttaaccc gattggcccg
    22261 acctgccata aaggcacctc aagctgcttc cgcgacacca gccaccagtg gctgttcctg
    22321 tatcagcttg agcagctgct ggccgagcgt aaaaacgctg acccggcaag ttcgtacacc
    22381 gctaagctgt acgccagcgg caccaagcgt atcgcgcaga aagtgggtga agaaggcgtg
    22441 gaaaccgcgc tggccgccac ggtgcacgat aagtttgagc tgaagaatga ggcgtcggat
    22501 ttgatgtatc acctgctggt gctattgcag gatcaggagc tggatttgac ggcggtgatt
    22561 gagaatttaa gggagcggca taagtaatcc ccccagtagg ttctttctgg gagtggccaa
    22621 aagataaacc ctaatgggtt tatcttttat ctcaacccaa taacatctct gctataattt
    22681 taatgtaatc attctacaat ctcgaataca gct
//
LOCUS       DAJSKJ010000054        21898 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000054 DAJSKJ010000000
VERSION     DAJSKJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.054"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(1..688)
                     /locus_tag="ON345_001774"
                     /pseudo
     CDS             complement(1..688)
                     /locus_tag="ON345_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
     gene            1602..1853
                     /locus_tag="ON345_001775"
     CDS             1602..1853
                     /locus_tag="ON345_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012005023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3071848.1"
                     /translation="MKVTSPTSLAYAIKDHRKINKLTQTKTAELIGIKQTTVSDFENR
                     PESTKLETLFKILSALDLELHVVKKGSTLDEKKVWTKEW"
     gene            1857..3158
                     /locus_tag="ON345_001776"
     CDS             1857..3158
                     /locus_tag="ON345_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HCR3071849.1"
                     /translation="MRTQTLTVAMNGDVVGTLYRDGSGAMSFQYAPEWLSVPGSRAIS
                     LSLPLQHSRIKGKEVFNFFSNLLPDSEAIIARMQTRFHVETAHPFDLLASVGRDCVGA
                     IQLYPPGSEIPTVMKTQAEPLDESQIEALLDDYQMAPLGMEEGADFRISLAGAQEKTA
                     LLWYQDRWQRPQGSTPTSHIFKLPIGRIEQNNIDLSESCENEWLCLRIAREFGFPVAN
                     ADLAVFGQKKVLIVERFDRRWSRDGWLMRLPQEDFCQALGVAPALKYESHGGPGIADA
                     MKLLLGSNLATQDREMFFKSQILFWMLAAIDGHGKNFSLFIEPESSFRMTPLYDVMSA
                     FPIFETGGIPLKKAKMAMALQGKNRQYHFSMIQPRHFISTAAHVGFSEDTAYKMMQEM
                     AVRTEEVIATVAAELPADFPEQISKAIFNGLSSQAARIIRE"
     gene            complement(3489..3932)
                     /locus_tag="ON345_001777"
     CDS             complement(3489..3932)
                     /locus_tag="ON345_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010846236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3071850.1"
                     /translation="MPGSPAFINSYLPALLGQAWLVVSSEFHTVVEARGFSVLEWRVL
                     SVLEGNGPTSVSELAMKAVCKQPTVTRLLHRLEKQQQVERCESQSGDRRLSWVTVTDT
                     GRQLVRELNAEAARFEEGVLGQLGEERSAMLKETLQTLIRRYSLA"
     gene            complement(3948..4646)
                     /locus_tag="ON345_001778"
     CDS             complement(3948..4646)
                     /locus_tag="ON345_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_053111714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HCR3071851.1"
                     /translation="METEIPAILRAREAIAPERFTFHSSRMRMKKVSKEELSSMDADS
                     DRCALELSDAAVDVLGYACLVAIMSMGKGYHRVSEERLYARTVENGHPSPVITSAGAL
                     VEGLQAIGAKRVSILTPYMKPLTQLVIDYIENEGIEVVDSLSLEIPDNLEVGRQDPLA
                     PVELTKKLNTNVDAIVASACVQMPSLPSIQLIEDRVGLPVISSSVATTWMMLNRLRLD
                     THVPGFGSLLSGRY"
     gene            complement(4703..5731)
                     /locus_tag="ON345_001779"
     CDS             complement(4703..5731)
                     /locus_tag="ON345_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR3071852.1"
                     /translation="MLELAKFTAAAVQAAPVFLDTEATVDKACNLIAEAAGNGAKLVA
                     FPEVFIAGYPYWSWVMNPVEGSPWFEKLCRSAIEVPGPEIRKIAQAAVRHKVNVVIGV
                     NERHPQSIATLYNTLVTINDEGRIIGRHRKLVPTWAEKLTWGNGDASSLRVHKTSIGP
                     LGALACGENTNTLARFSLLAQGELVHVASYIALPVAPKDYDMAEAIRLRASAHCFEGK
                     VFTVIACSTVSEEIVEAMAKTHPQARELLARPNSAFSGVIGPDGRVVGEPLIDKEGIV
                     YAEIDLNRCIQPRQMHDITGHYNRFDIFDLQVNRRAMNAATFHDSVESSPDFNSQNEA
                     AEPFQEQN"
     gene            complement(5746..6657)
                     /locus_tag="ON345_001780"
     CDS             complement(5746..6657)
                     /locus_tag="ON345_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR3071853.1"
                     /translation="MRLITYRSHVNAASRLGAIAGDYVVDLAALAHYSGKFLPDNMLD
                     FIDLGPSAIATGTEMLNACEGNWPVGCAQPLVNVKVLAPIPRPRKNIFGIGLNYVEHV
                     AESSRTLDTAKELPKEPVIFSKPPTSVIGPDDAIEHNAQITQQLDWEVELAVIIGSRA
                     KRVSEEQALNYVFGYSLMIDMSARDCRRAGQWIYSKGQDTFAPFGPCIVTADEIPDPH
                     NLNLSLRVNGVTKQSSNTRHMLFKVPTLIADISKGITLEPGDIIATGTPEGVGAGRSP
                     QEWVWPGDVIDAEVEYIGKLRHPVVAV"
     gene            complement(6657..7787)
                     /locus_tag="ON345_001781"
     CDS             complement(6657..7787)
                     /locus_tag="ON345_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR3071854.1"
                     /translation="MKYDDFRESTAGRADVEDTPELVAYYQQLSELKTGALWTVANKI
                     EPWQPKSSSVPVLWRYQDLREHVLRSVELVTPEKAGRRVIYLNNPGRQDVAAAVGWLY
                     SGLQVMHPGEAASAHAHSSSALRFIMEGRGAYTIVDGHKMPLEANDFVLTPNGTWHEH
                     GVEADGLPCIWQDGLDIPLVNAMEAGFYKVHPDLHQQVTAPVDDGVALWAAAALRPHN
                     VGWDKPYSPLFKYQWQPTYEALCRAAKVSDGSPFDDVLMHYTNPVTGGHVMPTIGASM
                     QRLRPGFVGKAHRHTGSFMYQVAKGKGYSVIDGQRFDWQERDIFCVPSWAFHEHVNLS
                     PTDDACLFCFNDLPVMQSLSLYYEEALLDNDGHQKIVSEEKK"
     gene            complement(7790..10081)
                     /locus_tag="ON345_001782"
     CDS             complement(7790..10081)
                     /locus_tag="ON345_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HCR3071855.1"
                     /translation="MNIVCIGGGPAGLYFALLMKLQNPANNIVVVERNRPYDTFGWGV
                     VFSDATLENLRQADPVSAEQIARQFNRWDDIEIHFKDSVNRSGGHGFIGIGRKKLLNI
                     LQERCQSLGVELVFDTLVEDEHALIERYQADLVLASDGINSRIRTRYQEVFQPDIDNR
                     QCRFVWLGTRKVFEAFTFLFAESPHGWFQVHAYQFEPGLSTFIVETTEETWKAVGLDQ
                     MSQEEGIAYCEKLFAPWLDGHPLMSNAAHLRGAAIWIRFPRVICQNWVHWVEHNKKPV
                     PLVLMGDAAHTAHFSIGSGTKLALEDAIELANSLQHTGGELRRGLDHYQAVRGVEVLK
                     IQNAARNSTEWFENVARYENFAPQQFAYSLLTRSQRISHENLRLRDPQWLSQYEQWLA
                     NAPDVPPILTPYQARSVVLKNRTVISPVLLYQAKEGVPGMFHLMHLGSRAVGGAGLVL
                     TEMTAVSPQGRITPNCPGLWNDEQVLAWRQVTDFVHQNSGASIGVQLGHSGRRGSTQR
                     GDEQPDHPLAQDNWPLISASPLPYRPGVSAVPAEATPAQLERVIAEFVDAARRAAKAG
                     FDWLELQAGHGFLLSSFISPLTNQRRDDYGGSLENRLRFPLAVFRAVREVWPQHLPIS
                     VRISATDWVEGGTTVDDAVEIARRFREAGADMIACSSGEVSVDQKPVYGRMFQTPMAD
                     RLRNEGGIATMAVGAISDADQVNGIIASGRADLCAIGRPALADANWLQRECARYGWNQ
                     VAWHPAYHYGRDLLQRALRPQEK"
     gene            complement(10078..10503)
                     /locus_tag="ON345_001783"
     CDS             complement(10078..10503)
                     /locus_tag="ON345_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR3071856.1"
                     /translation="MSATTFTQPHRIRFAECDPAGIVFYPQYFVMFNNLLEAWVDSLL
                     PYGFAGFIGERRFGMPTVHLEADFKAVSRMGDDVILQLEVIHIGSRSLKLALSCTSVE
                     GELRMQVTQTLVTTSLETHRSIDIPAELLAALQSSGEKL"
     gene            complement(10513..12000)
                     /locus_tag="ON345_001784"
     CDS             complement(10513..12000)
                     /locus_tag="ON345_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HCR3071857.1"
                     /translation="MPNVQPIKIAILAMGGEGGGVLADWIVNLGEENGYFAQTTSVPG
                     VAQRTGSTIYYVELFPGLYELDKPPVLALMPMPGDVDVVLASELMEAGRAVQRGLVTP
                     QRTTLIASTHRVYSIGEKTAMGDGRVNNDTLIEHANSASRKFIRFDMAKTAEESRSVI
                     SAVLFGALCGSGVLPFSQQQFEETITRGGVGVKTSLVAFAQGVMNARLEDAEPAVVTT
                     KAPQPSNPKVAALLARIHQELPESACPTAIEGVRRLLDYQDVNYAANYLDRLQTLSRQ
                     PGGDDELLLREAARHLALWMSWEDTIRVADLKIRGSRFERIRNDVRLQDNQHLSINEY
                     MHPRLEEICETLPAGLGRWLQKPGVCQRLVSKATRKGRVVTTSSLRGYLLLWTLSHWR
                     LGRRLTLRYQLEMARIDEWLQYVSRAAAFNPALALEIVQCQRLVKGYGDTHARGLRNY
                     QTLIGIVERHDCRLPPATLRELRDAALADEHGDKLRECQVRLALN"
     gene            complement(12012..14189)
                     /locus_tag="ON345_001785"
     CDS             complement(12012..14189)
                     /locus_tag="ON345_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HCR3071858.1"
                     /translation="MAERSFVEEVKKLTLPAGSVFSGEGILAVTKALLESGVSYVAGY
                     QGAPISHLMDVLADAQPILDEYGIRFENSASEATAAATLAASVNYPLRGAVTFKATVG
                     TNVASDALANLSSGGVLGGALIIVGEDYGEGSSIMQERSHAFAMKSQMWLLDPRPNLP
                     CIVQAVKDGFELSEASNTPVMLQMRIRSCHVHGQFVTAENRAPAFTVKDALANPQRDV
                     NRIVLPPASFLHEKEKIHQRWPAAVRFIRERRLNEFFSADADDIGIVLQGGNYNTLIR
                     ALNQLDLADVFGNSKIPLYVMNVAYPLVDEELDEFCRNKRAVLVLEEGQPNFVEQNIA
                     NILRQQELSTKLHGKDLLPMAGEYNTATVLAGLRAFFERYGRLAPDVQAAARQVRIPL
                     ANVMPDVVNAAPQPALADAIHARPPGFCTGCPERPIFTAMKLIERELGPHHISADIGC
                     HLFSILPPFNLGNTTMGYGLGGAGAAALNTKDGKRAISVMGDGGFWHNGLTSGIANSV
                     FNQSNNLTIVVDNSYTSATGGQDILSSTAHNATRSTGHAIEQAVKGVGVKWVKTVRRT
                     YDLKTMTATLRDALTSKTPGPKVLIAQSECMLNRQRREKQQVRKALESGKRVVAERFG
                     VDSDTCTGDHSCIRLSGCPSLSIKPNPDPLRTDPVATVINSCVGCGLCGEVSHAAVLC
                     PSFYRARIVTHPGRSERWREAIRRPLINWLQRRNARRLARVSL"
     gene            complement(14402..15685)
                     /locus_tag="ON345_001786"
     CDS             complement(14402..15685)
                     /locus_tag="ON345_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HCR3071859.1"
                     /translation="MTIAMTKSAVRPYAVTRRVLLVCWLSILFEGYDVGVMGAVLSSM
                     AEDAGWGLTPLELGALSSYALVGMFFGAFAAGSLSERIGRRRMLLLCVGLFSLSMIAT
                     TWAPTPFLFGVARFIGGLGLGGVIPVAAALTVEYSPPERRSYNYGIMYSGYSLGILCS
                     ALVAMWLIPHFGWRSVIGVGALPLLVLWPMSRLLPESLEYLVSRGRMDEARELSSRLG
                     VPLPEAETPRPEQEGWRKTGSLLFSREYLRATACFWVALFCGMLLVYGLNTWLPSIMR
                     KSGYALGPSLLFLVVFSLASAVGGLFLGRIADRIGAKRAVALSYLVGGIGICALMFPH
                     SLPVNILLTALAGVGSISASLILTGYLATWYPGNSRAAATGWALSFARVGAMSGPLFG
                     AWIASSGLSLSWNFGAFALVSLVAAVTVMLLPRGK"
     gene            16030..17037
                     /locus_tag="ON345_001787"
     CDS             16030..17037
                     /locus_tag="ON345_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3071860.1"
                     /translation="MLINDIVSPRLSAALSHLNSSANLLFTSATLEQAGAVVGRIFKP
                     CQVDLVDRSARADVRMHHIPMGEISLNRLRYSSRVDIAPCLMKDFFLVQMPLYGHATV
                     VSEGQEILSSPQLATVINPEDVTRMRWESGNDQFLLRISRSLLENMLVGQLGQPLNDP
                     LRFDAAFRWQENPAWCALVGYLLECASQGLDLEKHRLIRHQMEQLVATTLLNSHQHNY
                     RHPSDSKRSAIRPSHVRRVQEYIHAHAHEALTAEALAGIGGVSLRGLYAGFKNFLGVS
                     PMQYLRDIRMERVRAELLSGEVNNVTGAALRWGFAHMGRFSAEYKTRFGETPSQSMRK
                     R"
     gene            complement(17081..17866)
                     /locus_tag="ON345_001788"
     CDS             complement(17081..17866)
                     /locus_tag="ON345_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCR3071861.1"
                     /translation="MNKALLAEFSEGIAQGAIRIVDLTQTLSERFPTLQLPPQFGQAW
                     GFKKEAISHYDENGPGWYWNNFTCSEHTGTHFDAPAHWVSGREHQQNTVDTIDPCAFI
                     GPAVVVDASREVAEDPDWILSVDYLESWEKQYGKIPAGAWVLFRTDWSKKADDADAFL
                     NMREDGAHTPGPAQATVEWLIEQRNVRGFGVETINTDAGQSYAWSVPYPCHTLMHGAN
                     KYGLQCLTNLDKLPARGAVIVAAPLKIEQGSGSPLRVLALISE"
     gene            complement(17987..18454)
                     /locus_tag="ON345_001789"
     CDS             complement(17987..18454)
                     /locus_tag="ON345_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3071862.1"
                     /translation="MKSNVSSISPEGGTRDVKLWLRLLSSVNLIENVLRQRMREQFGS
                     TLPRFDMLAQLNRFPDGIKMGELSRLMLVTGGNITGLADSLEKEGMAVRVYDPSDRRS
                     CRIKLTAAGKHQFDLMATAHQQWLNELMGNLPGKEKDQLYTLLGNFKSQLPET"
     gene            18641..19789
                     /locus_tag="ON345_001790"
     CDS             18641..19789
                     /locus_tag="ON345_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR3071863.1"
                     /translation="MAETKVNRILNNNKLKLGFFAANCSGGMAVTKIEDRWNNSWENN
                     LKMAQLADEAGIEFLLPIARWIGYGGETNFHGNVLETVTWASALLAATRHISVFSTVH
                     TSFNHPVVVAKQLATMDHIGQGRAGLNVVCGWNRPEYEALGGDLAYDHESRYAYGQEW
                     FEVIRKLWREEKAFNWDGDFFKLKDTYGLPLPLQKDIPIFNAAGSGEGRDFAIRNADF
                     LFTSAITLERSAKEIAELKQKAIDAARQKALNILTFSYVVCRPTRAEAEEYHHYYSQQ
                     NADWAAVDNIIRLMFENAESFPKDQLKLIRDRFSAGHGGYPLVGTPDDVADGLERLYR
                     TGFSGTTLSFTDYVSEFPYFRDEVLPRLEARGLREPFRPYDSAAGEAA"
     gene            19786..20277
                     /locus_tag="ON345_001791"
     CDS             19786..20277
                     /locus_tag="ON345_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HCR3071864.1"
                     /translation="MSSLSADEFKLGMRQLTLPVAVITVHSDEQRNGLTASSFCSVSV
                     DPPRVLVCIGKSASAHRQLNVGEGIGINVLASEQLEHALRFASKEFHGEQRFAKGNWH
                     PLTSGAPMLEGTAVSFDCVVDQIIDDTTHSIVTATVVAVERSNNDCLLYRNGAPVQTS
                     SIA"
     gene            20400..21404
                     /locus_tag="ON345_001792"
     CDS             20400..21404
                     /locus_tag="ON345_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR3071865.1"
                     /translation="MSSSFTRLPRGARGLLALILVASASAEATTPSVTEPAGINLGLT
                     SFYDGFPAAAPGQLSYLGYYRYTHANAIKDNAGNKIDAFGKSNVDTWLTSHQFLYTFA
                     HSPFDNASLGLDLIIPIVGFNTDFGDAPAKLQANDAGFGDIFVGTYLQMNPVMDTQGT
                     PLFAQRFEIGFNVPTGKYDAHQDINPGANQWSFTPTWAATWLFAPRASLNWRLQYLVN
                     FNNTRPASSTPLDWKGESVERTRAGQLAWLNFAAEYEVAPKWHLGINGYYLKQISDDK
                     VNGDDLPNSREQVLGIGPGVMWNHSPNNTLTLNFYTETAVENRLRNSAIVNLFYIHGF
                     "
BASE COUNT         4829 a         6408 c         5943 g         4718 t
ORIGIN      
        1 taggtcacat caccgcacca cacctgattt ggctcggtca cggcgaactg ccgttcaagg
       61 tagttaggga tagcaacatg ttcatgacca ccacgtttat accggtaagt cggctgctga
      121 cagctgacca gccccagctc tttcatgagt ctgccagcaa gccagcgccc catctggtag
      181 cctctctggg ttgccattgt ggcgatgctt cttgctccgg cagagccgtg gctgatgcca
      241 tgcagttcaa gtacctggct gcgtaataca gcccgtctgc cgtctggctt ttcaggacgg
      301 tttttccagt atttgtagct gctgcgatgg accccgaaca catggcagag agtggccaca
      361 ggataacgcg ccctgagttt cccgattatc gagaactgtt cagggagtct gacatcaaga
      421 gcgcggtagc cttttttaat atttcatttt ccatttcaat acgttgtagc tttttcctga
      481 gctcacggat ttcaatttgt tccggggtaa tgggggaggc ttttggtgtt tttccctgcc
      541 gctcatcacg taattgtttc acccatcgcg tcattgtgga aaggccgaca tccatagcgc
      601 tggctgcatc tgccacggtg taattctggt caacgaccag ttgagcggat tcgcgtttaa
      661 actctgcgct gaaatttctt tttttcatta tggcacctgt gttgttctga ggtgagcata
      721 tcacctctgt tcaggtggcc aaattcagta aaccacttca tttatcttag actatctatc
      781 gagcatagtt tgtgcaactt tctgatttga agcaatagaa gccataaagc cccgttggtg
      841 atacagcatc aagcggagcg tagcgacttc ctcagattgt cagagagatc tcaccttatc
      901 aggataaccg tgtacccttg cgggagcgtg gttgaaacgc tgatagagcc tacatatcgt
      961 ttgtaggtac gttatcacca taaacctgtt tacgtggctc ataaggccgc acatcgggct
     1021 aaaatgcgtg tgatcaatct gttttggtat ggagttttga gacggcggca cgtcttccgg
     1081 cttcaaagga aaagcgatac aaggttgagc ctggtaacta cttgccactg cgtgctaaca
     1141 taccaaagcc attaacaatc acagtggctt ttgtacttga tagggaactg acatttctta
     1201 tatgcaaatg aggcaggttt ttcacgctaa ctcattgtat ttaaaggaat ttagtgactt
     1261 ggtgccgaag gccggactcg ccttttcttt caatctattg aaaattaatt atttatgtaa
     1321 ttctcaaatt taaactagcc cccaaaaccg gcctcaaagc aggcttattg agcaacttca
     1381 tacaaatttt actgactaat gcctgaaagt agagactagt cacagtccgt aaaaatcgta
     1441 aatactggcc atgattctct cgcgtaaaca accaatatca tcatttacac aggcatcttt
     1501 accctcttta gaggataaat tgaatttaac ccccataggg gttaatcttg ttttaccccc
     1561 tataggggtt tttatttaaa tatccgctaa aggggataat gatgaaggta acatctccaa
     1621 cctcactggc ttatgcgatc aaagaccatc gtaaaatcaa taagttgacc caaacaaaaa
     1681 ctgctgagct tatcgggatt aaacaaacaa ctgtctctga tttcgaaaac agacctgaat
     1741 ccaccaaact ggagacattg ttcaaaatcc tttcagccct ggatcttgag cttcatgttg
     1801 tgaaaaaggg ctcaacgctg gatgaaaaaa aagtctggac gaaagagtgg taaaacatga
     1861 gaacacaaac gctgacagtc gcaatgaatg gcgacgtggt gggcaccctg taccgggatg
     1921 gcagcggtgc gatgtcattt cagtacgccc ctgagtggct gtctgtaccc ggttcacgcg
     1981 caatctcatt atcacttcct ctccagcaca gcagaataaa gggaaaagag gtcttcaact
     2041 tcttcagcaa cctgctacca gattccgagg caattattgc caggatgcag acccgcttcc
     2101 atgtagaaac agcgcaccct ttcgatttat tagcgagtgt cgggcgtgac tgcgtgggtg
     2161 ccattcagct ttatccgccc ggaagtgaaa tccccacagt gatgaaaacg caggctgagc
     2221 cacttgacga atctcagatc gaagcgctgc ttgatgatta ccagatggcc cccctgggga
     2281 tggaagaagg agctgatttt cgtatctcat tagccggagc ccaggaaaaa acggcgctgc
     2341 tctggtatca ggatcgctgg caaaggccgc agggcagcac accgaccagc catattttca
     2401 aacttccgat tggcagaata gaacaaaaca acattgatct gagtgaaagc tgcgagaatg
     2461 aatggctgtg tttgcgtatt gccagggaat ttggcttccc ggttgctaat gcggatctgg
     2521 ctgtatttgg tcaaaaaaag gtgctgattg tcgagcgttt tgaccggaga tggtcccgcg
     2581 atggctggct gatgcggctc ccacaggagg acttttgcca ggcactggga gttgctccgg
     2641 cgctgaaata tgaatcacac ggtggccccg ggatcgcgga tgccatgaaa ttgctgttag
     2701 gctcaaacct tgctacccag gatcgcgaaa tgttctttaa atcgcagatc ctcttctgga
     2761 tgctggccgc aatagacggt cacgggaaga attttagcct gttcatcgag ccggaatcat
     2821 catttcggat gaccccttta tatgatgtca tgtccgcatt cccaatcttt gaaacaggtg
     2881 gtatcccctt aaaaaaagca aagatggcca tggcattaca aggtaagaat cgacagtatc
     2941 atttctcaat gatccagccc cggcacttca tcagcaccgc cgcacatgtc ggattctctg
     3001 aagacacagc atacaaaatg atgcaggaaa tggccgtccg tacagaagaa gtcattgcga
     3061 cggttgcggc ggagctacca gcagacttcc ccgagcaaat aagcaaagcc attttcaatg
     3121 gattatccag ccaggcggcg cgaattatca gagagtaacg gttgaggcag cacccagaca
     3181 ttagatcgta ctccgacctg atgtctgggc cttgactatt attgatgcag tactaatttg
     3241 cgagcaaatc ccggcatcgc acaatgtact cgtgaaatcg tagtacgaga atcctggata
     3301 attgtatatc gcatagaacc gaagaagctc gttattttgc gcgtattaat ttcacaaaaa
     3361 atatccttag tcacagcttg atatatcaca agagagatag cactttcgtc acctcaccgg
     3421 tgcaccacgg tccgcgaaca ataacgggag gagcccatac atggccgagt gccgcgacgc
     3481 caggaaaatc atgccaggct gtaacgccga attagcgtct gcagcgtctc tttcaacatc
     3541 gcgctacgct cttcccccaa ctgacctaac acgccttctt caaagcgtgc cgcctccgcg
     3601 ttcagttcgc gcaccagttg ccgcccggta tctgtcaccg tcacccagct aagccgtcta
     3661 tcgccactct ggctttcaca gcgttccacc tgctgttgct tctcgagtcg atgcagcagc
     3721 cgggtgacgg tcggctgctt gcacaccgcc ttcatcgcca gctcgctgac actggttggt
     3781 ccattgccct ccagcaccga gagcacgcgc cactccagca ccgaaaaacc ccgagcctcc
     3841 accaccgtat gaaactcaga tgacaccacc aaccacgcct gcccgagcag cgcgggtaaa
     3901 tagctgttaa tgaacgccgg tgagcctggc atctgtacct cctgcgatta ataacgtccg
     3961 gatagcagcg aaccaaaccc cggcacgtgc gtatccagac gcagacggtt aagcatcatc
     4021 caggtggtgg cgacggacga cgaaatcacc ggcagcccga cgcgatcttc aatcagttga
     4081 atcgagggca gcgacggcat ctgcacgcag gccgaagcga caatcgcatc gacattggtg
     4141 ttcagctttt tggtcagttc aaccggcgcc agcggatcct gacggccaac ttccaggtta
     4201 tcggggatct ccagcgacaa gctgtccaca acttcaatcc cttcgttttc aatgtagtcg
     4261 ataaccagtt gggtcagcgg cttcatatac ggcgtgagga tcgatacgcg cttcgcgcca
     4321 atcgcctgta gcccttccac caacgcgccc gcgctggtga tcaccggcga cggatggccg
     4381 ttttccacgg tgcgcgcata cagacgctct tccgacaccc gatgatagcc tttacccata
     4441 ctcatgatcg ccaccagaca ggcataaccg agaacgtcca cggcggcatc ggaaagctcc
     4501 agtgcgcagc ggtcgctatc ggcgtccatc gacgacagct cttctttcga cacttttttc
     4561 atgcgcatac ggctggagtg gaaggtaaaa cgctctggcg caatcgcctc tctggcacgc
     4621 agaatcgccg gaatctccgt ctccatggtg gtattcgacg acgggacaat ctgcccaata
     4681 cggaaatgac gtggggtctg catcagtttt gctcctggaa cggctccgcc gcttcattct
     4741 gactgttgaa atccggtgaa ctttcgacag agtcatggaa agtcgccgca ttcatggcac
     4801 gacggttgac ctgcaaatcg aaaatatcga aacggttata gtggccggtg atgtcatgca
     4861 tctgccgcgg ttggatgcag cggttgaggt cgatttcggc gtagacgatg ccctctttat
     4921 caatcaacgg ttcgcctacc acgcgcccgt cagggccaat caccccagag aatgcgctgt
     4981 ttggccgcgc cagcagttca cgcgcctgcg gatgcgtttt cgccattgcc tcaacgatct
     5041 cttcgcttac ggtcgagcaa gcgataaccg taaacacttt tccttcaaag caatgtgccg
     5101 aagcacgcag gcgaatcgcc tccgccatgt cgtaatcctt aggtgctacc ggtagtgcga
     5161 tatagctggc gacgtgcacc agttcgccct gcgccagcag cgaaaaacgc gccagcgtat
     5221 tcgtattttc accgcaggcc agcgcgccca aaggcccgat gctggttttg tgcacccgca
     5281 gcgaagacgc atcgccgttg ccccacgtca gcttctccgc ccaggtcggc accaatttgc
     5341 ggtgtcgacc aataatcctg ccttcatcgt tgatggtcac cagcgtgtta tagagcgtgg
     5401 caatgctctg cgggtggcgc tcattgacgc caatcacgac attcaccttg tggcgcacgg
     5461 cggcctgggc gattttacga atctccggcc ccggtacttc aatcgcgcta cggcacagtt
     5521 tttcaaacca cgggctgcct tcaaccgggt tcatcaccca gctccagtac gggtaaccgg
     5581 cgataaacac ttccgggaag gccaccaact tcgcaccgtt gcccgccgct tcggcaatca
     5641 ggttacacgc tttgtcgacg gtcgcctcgg tatcaagaaa taccggcgcg gcctgcacgg
     5701 ctgctgcggt aaatttggct aattccagca tcatctgttc tcctctcaga ccgcgaccac
     5761 cgggtggcgt agcttaccga tgtactcgac ttcggcatca atcacatcac ctggccacac
     5821 ccactcttgc gggctgcgcc ccgcccccac gccctccggc gtgccggtag cgatgatgtc
     5881 gcccggctca agggtgatcc ctttactgat gtcggcaatc agcgtcggta ccttgaacaa
     5941 catgtggcga gtattggaac tttgttttgt cacgccgtta acccgcaggc tgaggttgag
     6001 attatgtgga tccgggattt catcagcggt gacgatacat gggccaaacg gtgcaaaggt
     6061 gtcctgccct tttgagtaga tccactgtcc ggcacgacgg cagtcgcggg cgctcatgtc
     6121 gatcatcagg ctgtaaccga aaacatagtt cagcgcctgt tcttcgctga cgcgcttagc
     6181 gcgggagccg ataatcaccg ccagttcaac ttcccagtcc agttgctggg tgatctgcgc
     6241 gttgtgctca atggcatcgt ccggaccaat caccgaagtc ggcggcttgg agaaaatcac
     6301 cggttctttc ggcagctctt tcgccgtatc gagcgtgcgg ctggactcgg caacgtgctc
     6361 aacatagttc agaccgatac cgaagatgtt tttgcgcggg cgagggatcg gcgccagcac
     6421 cttcacgttg accagcggct gggcgcagcc caccggccag ttgccttcac aggcgttaag
     6481 catctcagta ccggtggcta tcgctgacgg ccccagatcg atgaaatcca gcatgttatc
     6541 cggcagaaac ttgccgctgt agtgcgcaag cgcagctaaa tcgaccacat aatccccggc
     6601 gatagcgccc aggcgagatg cggcgttaac gtggctacgg taggtaatca gacgcattat
     6661 tttttctcct cagacacaat tttttgatga ccgtcgttgt ccagcagcgc ttcttcgtaa
     6721 tagagactga gcgactgcat cactggcaga tcgttaaaac agaacaggca cgcatcatcg
     6781 gttggcgaca ggttgacgtg ctcatggaag gcccaggacg gcacgcagaa aatgtcgcgt
     6841 tcctgccagt caaagcgctg cccgtcgata accgagtagc ctttgccttt cgccacctga
     6901 tacataaagc tgccggtatg gcggtgcgcc ttgccgacaa agccgggacg caaacgctgc
     6961 atactggcgc cgatggtcgg catcacgtga ccgccggtca ccgggttggt gtagtgcata
     7021 agtacatcgt caaacggtga gccgtcgctg accttcgctg cgcggcacaa cgcttcataa
     7081 gtaggttgcc actgatactt aaacagcggc gaatagggtt tatcccagcc aacgttatgc
     7141 ggccgcaatg ccgcggcggc ccatagcgcc acgccgtcat cgaccggcgc ggtgacctgc
     7201 tgatgtaaat ccgggtgcac cttgtagaat ccggcctcca tcgcgttgac caacggaata
     7261 tccagcccgt cctgccagat gcagggcaaa ccgtccgctt ccacgccgtg ttcatgccag
     7321 gtgccgttcg gcgtcagcac aaagtcattg gcctccagcg gcatcttgtg accatcgacg
     7381 atggtgtaag ccccgcgtcc ttccataatg aagcgcagcg cggaagagga gtgcgcgtgc
     7441 gccgaggccg cttcgcccgg atgcatcacc tgaaggccgg agtagagcca gcccactgcc
     7501 gccgccacgt cctgacgacc agggttattc aggtaaatca cccgacgccc cgctttctcc
     7561 ggggtgacca gctccaccga acgcagtacg tgctcgcgca gatcctgata gcgccacagt
     7621 accggcaccg acgatgattt cggctgccac ggttcgattt tgttcgccac cgtccacagc
     7681 gcgccggttt tcagctcaga aagttgctgg taataagcca ccagctccgg ggtatcttct
     7741 acatccgcgc gaccggcggt gctttcgcga aagtcgtcat atttcatgtt catttctcct
     7801 gtggacgtaa cgcgcgctgc aacagatcgc gaccatagtg ataggccgga tgccaggcca
     7861 cctgattcca gccgtagcgg gcgcattcac gctgtaacca gttggcgtcc gccagcgccg
     7921 ggcggccaat ggcgcagagg tcggcgcgcc cggaggcgat aatgccgtta acctgatccg
     7981 cgtcgctgat tgcccccacc gccatggtgg cgatcccgcc ttcgttacgc agacggtcag
     8041 ccatcggcgt ctggaacata cgcccatata ccggcttttg atcgaccgag acttcgccgg
     8101 aggagcaggc gatcatgtct gcgcccgcct cacggaaacg acgcgcgatt tccaccgcgt
     8161 cgtccaccgt tgtcccccct tccacccagt cggtcgcgga gatacgtacc gaaatcggta
     8221 aatgctgcgg ccacacttcg cgcactgcgc ggaacaccgc cagcgggaaa cgcagacggt
     8281 tttccagcga gccgccgtag tcgtcacggc gctggttggt cagcggcgaa ataaagctgg
     8341 agagcagaaa accgtggccc gcctgaagct ccagccagtc aaatccggct ttggcggcgc
     8401 ggcgcgcggc atcgacaaat tcggcaatca ccctctccag ttgtgcaggc gtcgcttcag
     8461 cgggcacggc ggaaacgcct gggcggtaag gcagcggtga agcggagatc agaggccagt
     8521 tatcctgcgc cagcggatga tccggctgtt catcgccacg ctgggtggaa ccgcgacgcc
     8581 cggaatggcc cagctgtacg ccgatgctcg cgccgctatt ctggtggaca aaatcggtca
     8641 cctgtcgcca ggcaagcacc tgctcgtcat tccacagccc cggacagttc ggcgtgatgc
     8701 gcccctgcgg cgagacggcg gtcatttctg tcagtaccaa ccccgcgccg ccaacggcgc
     8761 ggctgccaag atgcatcaga tggaacatgc ccggtacgcc ctctttcgcc tgatacagca
     8821 gcacgggtga aatcacggtg cgatttttca gcaccacgct gcgcgcctgg tatggcgtca
     8881 gaatcggcgg cacatccggc gcgttcgcca gccactgttc gtactggctc agccactgcg
     8941 ggtcgcgcag acgcaggttc tcgtgggaaa tacgctgtga gcgagtcagc agtgaatagg
     9001 caaattgctg cggggcaaag ttctcgtagc gggcgacgtt ctcaaaccac tcggtggagt
     9061 tacgcgccgc gttctggatt ttcagtactt ccacgccgcg taccgcctga tagtggtcga
     9121 gcccacggcg cagctcgcca ccggtatgtt gcaggctatt cgcaagctca atggcgtctt
     9181 ccagcgccag cttggtgccg gagccaatcg agaagtgcgc ggtgtgggcg gcatcgccca
     9241 tcagcaccag tgggacaggc ttcttgttat gttcgaccca gtgcacccag ttctggcaaa
     9301 tcacgcgcgg gaaacggatc cagatcgccg cgccgcgcag gtgggcggcg ttggacatca
     9361 gcggatgacc gtccagccac ggcgcaaaaa gcttctcgca gtaggcgatg ccctcttcct
     9421 gactcatctg atccagcccg acggctttcc aggtctcttc ggtggtttcg acgataaaag
     9481 tcgaaaggcc cggttcaaac tgataggcgt gcacctggaa ccagccgtgc ggcgattcgg
     9541 cgaataaaaa ggtaaaggct tcaaacacct tgcgtgttcc gagccagaca aaacgacact
     9601 ggcgattatc aatatccggc tggaacacct cctgatagcg ggtacgaatg cggctgttaa
     9661 tcccgtccga agccagcacc agatccgcct gataacgttc aatcagcgca tgttcgtctt
     9721 ccaccagcgt gtcgaacacc agttcaacgc cgagcgactg gcagcgctcc tgcaaaatat
     9781 tgagcagctt tttgcggcca ataccgataa acccgtggcc gccgctgcgg ttcacggaat
     9841 ctttaaaatg gatttcgata tcgtcccagc ggttgaactg ccgggcgatc tgttcagcgc
     9901 tcaccgggtc ggcctgacgc agattttcca gcgtcgcgtc ggagaacacc acgccccagc
     9961 cgaaggtgtc atacgggcgg ttacgctcca ccaccacaat gttattcgcc ggattttgca
    10021 gcttcatcaa cagggcaaaa tagagccccg ccggaccgcc gccgatacag acaatattca
    10081 tagcttctct cctgatgact gcaatgcggc cagcagctcc gccgggatat cgatcgaacg
    10141 atgggtttca agggaggtgg tcaccagcgt ctgggtcacc tgcatccgca gctcgccctc
    10201 aacgctggtg cagctcagcg ccagcttcag cgaacgcgag ccaatgtgga tcacttccag
    10261 ctggaggatc acgtcgtcgc ccatgcggct gacggcttta aaatccgcct ccagatgcac
    10321 cgtcggcatc ccgaaacggc gctcgccgat aaatccggcg aaaccgtacg gcagcagcga
    10381 atcgacccac gcctccagta agttgttgaa catcacgaaa tattgcgggt agaaaacaat
    10441 ccccgccgga tcgcactcgg caaagcgaat gcgatgcggt tgggtaaagg tcgtagcact
    10501 cataaaagct ccttagttca gcgccagacg cacctggcac tcgcgcaatt tatcgccatg
    10561 ttcatccgcc agcgccgcgt cgcgcagttc gcgcagggtg gcaggcggca gacggcaatc
    10621 gtggcgctcc acgatgccga ttaaggtttg gtaattacgt aatccacgtg cgtgggtatc
    10681 gccatagccc ttcaccagcc gctggcactg cacaatttcc agcgccagcg ccgggttaaa
    10741 ggccgccgcc cggctgacgt attgcagcca ttcatcaatg cgcgccattt ccagctgata
    10801 gcgcagggtc aggcgacgcc ctaagcgcca gtgcgacagc gtccacagca gcaggtagcc
    10861 gcgcagcgaa ctggtggtca ctacccgccc tttgcgcgtg gctttgctca ccagacgctg
    10921 gcaaacgccc ggtttttgca gccagcggcc cagcccggcg ggcagggttt cacagatttc
    10981 ttccagccgc gggtgcatgt attcgttgat gctcagatgc tggttatcct gcaaccgcac
    11041 gtcgttgcgg atacgctcaa agcggctacc acgaatcttc aagtcagcca cgcgaatggt
    11101 gtcctcccac gacatccaga gagccagatg gcgcgcggct tcgcgcagca acagttcatc
    11161 gtcgccgcca ggttgacggg agagcgtttg caggcgatcg agatagttcg ccgcataatt
    11221 cacgtcctgg taatcaagca ggcggcgcac gccttcaatg gcggtaggac acgcgctttc
    11281 cggcagttcc tgatggatac gcgccagtag ggcagccact ttcgggttcg aaggctgcgg
    11341 cgctttggtg gtcaccactg ccggttcggc atcctccaga cgggcattca tcaccccctg
    11401 agcgaaggca acaaggctgg ttttcacccc cacaccgccg cgggtaatgg tctcttcaaa
    11461 ctgctgctgg ctgaacggca gcacgccgga accgcacagc gcgccgaata acacggcgct
    11521 tatcacgctg cggctttctt ccgccgtttt cgccatgtcg aagcgaataa atttccgcga
    11581 tgcgctgttg gcgtgctcga tcagcgtgtc gttgttgacg cgcccgtcgc ccatcgccgt
    11641 tttctcgccg atggaataga cgcggtgcgt ggaggcgatc agcgtagtgc gctgcggcgt
    11701 caccagcccg cgctgcaccg cgcgcccggc ttccatcagt tcggaagcca gcaccacgtc
    11761 aacgtcaccc ggcatcggca tcagtgccag caccggaggc ttatccagtt catacagacc
    11821 cggaaacagc tcgacgtaat aaatggtgga gccggtacgc tgcgctacgc ctggcaccga
    11881 ggtggtctga gcgaaatagc cattctcttc tcccaggttg acgatccagt cggcgagcac
    11941 gccgccccct tccccgccca tcgccagaat ggcgattttg attggttgta cattcggcat
    12001 tcgagcgttc cttacaggct gacacgggca aggcgacggg cgttccggcg ctgcaaccag
    12061 ttgattaacg ggcgacgaat cgcctctctc cagcgttcgc tacgccccgg atgggtgacg
    12121 atgcgcgccc ggtaaaacga cgggcagagc acggcagcgt gcgacacttc gccgcacagg
    12181 ccgcaaccga cgcagctgtt gataaccgtg gcgaccgggt cggtacgcag cggatcggga
    12241 tttggcttga tcgacagcga cggacagccc gaaagacgaa tgcaggagtg atcgccggta
    12301 caggtatcgc tatcgacgcc gaagcgctcc gccaccaccc gtttgccgct ctccaacgct
    12361 ttgcgcacct gctgcttctc gcggcgttgg cggttgagca tgcattcgct ctgggcaatc
    12421 aacaccttcg gccccggcgt tttgctggtt aaggcatcgc gcagggtggc ggtcatggtt
    12481 ttcaggtcgt aggtacggcg caccgttttc acccacttca cgccaacgcc tttcaccgcc
    12541 tgctcgatgg cgtggccggt gctacgggtg gcgttgtggg cggtagatga gagaatatcc
    12601 tgaccgccgg tggcggaggt gtagctgtta tccaccacga tggtcaggtt gttgctctgg
    12661 ttaaatacgc tgttggcgat gccgctggtc aggccgttgt gccagaagcc gccgtcgccc
    12721 atcaccgaaa tggcgcgttt gccgtctttc gtgttaagcg ctgccgcccc tgcgccgcca
    12781 aggccgtagc ccatggtggt gttgccgagg ttgaacggcg gcagaatcga gaacaggtgg
    12841 cagccgatat cggcgctgat gtggtgcgga cccagctcgc gctcaatcag cttcatggcg
    12901 gtaaagatcg ggcgctccgg gcagccggta cagaatcccg gcggacgggc gtgaatggcg
    12961 tcagccagcg caggctgcgg cgcggcgttc accacgtccg gcatcacgtt ggccagcgga
    13021 atgcggacct gacgcgcagc ggcctgaaca tccggcgcca gacggccata gcgttcaaag
    13081 aaggcacgca gccccgccag caccgtcgcg gtgttgtact caccggccat cggtagcagg
    13141 tccttgccgt gcagtttggt gctcagttcc tgctggcgca ggatattggc gatgttctgt
    13201 tcaacaaagt tcggctgccc ctcttccagc accagcaccg cgcgcttgtt gcggcagaac
    13261 tcgtccagct cttcatccac cagcggatag gcaacgttca tcacgtacag cggaattttg
    13321 ctgttgccga acacgtcggc cagatccaac tggttcaggg cgcggatcag cgtgttgtag
    13381 ttgccgccct gcagcacaat gccgatatca tcggcatcag cggagaagaa ttcattcagg
    13441 cggcgttcgc ggataaagcg caccgctgca ggccatcgct gatgaatttt ctctttttcg
    13501 tgcaggaagc tggcgggtgg cagcacaatg cggttcacat cgcgctgcgg gttggctaag
    13561 gcatctttca ccgtaaacgc cggggcgcgg ttttccgcgg tgacaaactg gccgtgaacg
    13621 tggcaggagc gaatgcgcat ttgcagcatt accggggtgt tgctggcttc agacagctca
    13681 aaaccgtcct tcaccgcctg tacaatacac ggcaggttcg ggcgcgggtc gagcagccac
    13741 atctgcgatt tcatggcgaa cgcgtggctg cgctcctgca tgatggagga accttcgccg
    13801 taatcttcgc cgacgataat cagcgccccg cccagcacgc cgccggagga gaggttcgcc
    13861 agcgcgtcgg aggcgacgtt ggtgcccacc gtcgctttaa aggtcaccgc gccgcgcagc
    13921 ggatagttca ccgaagcggc aagcgttgcc gccgcagtgg cttcgctggc gctgttttca
    13981 aaacggatcc cgtattcgtc gaggatcggc tgagcgtctg ccagcacgtc catcaggtga
    14041 gagataggcg cgccctgata gcccgccacg taagagacgc cggattccag tagcgctttg
    14101 gtgacggcca gtatgccttc gccgctaaat acggaacctg ccggtaaggt gagtttcttt
    14161 acctcttcaa caaacgaacg ctcagccatt gcaacctcac tttcatttct aattggtctg
    14221 gattacttgt aagcaaggcg tgaaggcggg gcaatccatg atttgtcatg gaattgtcat
    14281 ttgtgcggaa gatggataac gattgcagaa gggggataaa aaggggggtt ttaaggcact
    14341 ggcacgatct gccccctctc cccaaatggg agagggaacc cagaggtgcg gtttttggcc
    14401 tttattttcc acgcggcaac aacatcaccg tcaccgccgc caccagtgac accagtgcaa
    14461 atgcgccaaa gttccacgac agcgaaagcc cgctgctggc aatccacgcg ccgaacaacg
    14521 gcccgctcat cgcccccacg cgggcgaagc tcagcgccca gcccgtcgcc gccgcgcggc
    14581 tgttgcccgg ataccaggtc gccagatagc cggtcagaat cagcgaggcc gaaatgctcc
    14641 ctacccccgc cagtgcggtc agcaaaatgt taacgggcag cgaatgcggg aacatcagcg
    14701 cgcagatgcc tatgccgccc acgaggtaag agagcgccac cgcccgcttc gcgccaatcc
    14761 ggtcagcgat acgcccgagg aacagcccac ccaccgctga ggcgaggcta aacaccacca
    14821 gaaacagcag gctcggcccc agcgcatagc cgcttttgcg cataatgctc ggcaaccagg
    14881 tgttgagacc gtagaccagc aacatgccgc agaatagcgc cacccagaag caggccgtag
    14941 cccgcagata ctcgcgcgag aacaacagac tgccggtttt gcgccagccc tcctgctccg
    15001 gtcgcggcgt ttcagcttcc ggcagcggta cgcctaagcg tgacgagagt tctcgcgctt
    15061 cgtccattcg tccccggctc accagatact ccagcgattc cggcaacaag cgcgacattg
    15121 gccacagcac cagtagcggc aacgcgccaa cgccaatcac gctgcgccag ccgaagtggg
    15181 ggatcagcca catcgccacc agcgccgagc agagaatgcc cagcgaatag ccggaataca
    15241 taattccgta gttgtagctg cggcgttccg gcggcgagta ctccaccgtc agcgccgccg
    15301 ccaccgggat cacaccgccc agacctaacc cgccgataaa gcgcgccacc ccgaataaga
    15361 acggcgtcgg cgcccaggtg gtggcgatca tcgagagcga aaacagcccc acgcacagca
    15421 acaacatgcg tcgccgacca atacgctcac tcagcgaacc cgcggcaaac gcaccgaaga
    15481 acattcctac cagcgcgtag ctactgagcg cccccagctc cagcggtgtt agcccccagc
    15541 ctgcgtcttc cgccatgctg gagagcaccg cccccatcac gcccacgtcg taaccttcaa
    15601 ataaaatact cagccaacac accagcaata cccggcgggt aacggcatac ggacgcaccg
    15661 cactctttgt catcgcaatt gtcatggcaa actccggtta gtgaacacgc catcagcgat
    15721 aagaaaagcg acgcaaagga tcaatgcttt cgcgggatgc cagccgttat atctgcataa
    15781 cccggataat cacggcgcaa aatggcatct cgcctccctc agcgataaca aaatctgcgc
    15841 gatccctctc tcattatcgg aatttgctct gacatcactt taaaaatccg gttaacgtat
    15901 gtgcattatt gatttgttaa ctatgtatta atggaatgtt gattgaagca acgcattagc
    15961 gtatgacggc cataaatcaa caacaggcag gacgtttcac ctttgcgtca tctccacgag
    16021 gagtctgtta tgttgatcaa cgatatcgtt tcgccgcgcc tctccgctgc gctgagtcat
    16081 ctcaactctt ccgccaactt gctgtttacc tcggccacgc tggagcaagc gggcgcggtg
    16141 gtcgggcgga tctttaaacc ttgtcaggtg gatctggtcg atcgcagcgc gcgcgccgac
    16201 gtgcgtatgc accacattcc gatgggggaa atctcgctta atcgcctgcg ctacagctcg
    16261 cgggtagata tcgccccctg cctgatgaag gatttttttc tggtgcagat gcccttgtac
    16321 ggccacgcca cggtggtgag cgaaggccag gagatcctct ccagcccgca actggcgacg
    16381 gtgatcaatc cggaagatgt gacccgtatg cgctgggagt caggtaacga tcagtttttg
    16441 ctgcgcatct cccgctcgct gctggaaaac atgctggtcg gccagctcgg tcagccattg
    16501 aatgatccgc tgcgtttcga tgcagccttt cgctggcagg agaatcccgc ctggtgcgcg
    16561 ctggttggtt atctgctgga gtgcgccagt cagggactgg atctggaaaa gcaccgtttg
    16621 atccgccatc agatggaaca actggtggca actacgctgc tcaacagtca tcagcacaac
    16681 taccgccacc cctccgacag taaacgctcg gcgattcgtc catcccacgt gcgtcgcgta
    16741 caggagtata ttcacgccca cgcgcacgaa gcgctgacgg cggaggcgct ggcgggcatt
    16801 ggcggcgtta gcctgcgcgg cctgtatgcc gggtttaaaa actttctcgg cgtaagccca
    16861 atgcagtatc tgcgggatat tcgtatggag cgggtgcgcg cggaactgct ctccggcgaa
    16921 gtgaataacg tgaccggtgc ggcactgcgc tggggattcg cccatatggg gcgctttagc
    16981 gcggagtata agacgcgctt tggcgagacg cccagtcaaa gcatgcgtaa acgctgactg
    17041 ggcaatacac gtcatacttc acgttgccga tgcgttgaag ttattcgcta atcagcgcca
    17101 gtacgcgtaa tgggctaccg gaaccctgct cgattttgag cggggccgcg acgatcaccg
    17161 caccgcgcgc cggtagtttg tcgaggttgg tcaggcattg caggccatat ttgttggcgc
    17221 cgtgcatcag cgtgtggcac ggatacggca ccgaccacgc gtaggattgc ccggcgtcgg
    17281 tgttgatggt ttccaccccg aagccgcgca cgttgcgctg ctcaatcagc cactccacgg
    17341 tcgcctgcgc tgggcccggc gtgtgagcgc catcttcacg catgttgagg aacgcgtcgg
    17401 catcatcggc ctttttcgac cagtcggtgc gaaacagcac ccatgcgcct gccgggattt
    17461 taccgtattg cttttcccag ctttccagat aatcgaccga caggatccag tccggatctt
    17521 ccgccacttc acggctggca tcgaccacca ccgccgggcc aataaatgcg cagggatcga
    17581 tagtatcaac ggtattttgc tgatgctcgc gaccgctcac ccagtgcgcg ggcgcgtcaa
    17641 aatgggtgcc agtatgttcg ctacaggtaa agttgttcca gtaccagccg gggccatttt
    17701 cgtcatagtg agaaatcgcc tcttttttga agccccaggc ctgaccaaac tgcggcggca
    17761 gctgcagcgt cgggaaccgt tcgctgagcg tctgcgttaa gtcgacaatc cggatcgcgc
    17821 cctgagcgat cccctcagaa aattcagcca gcaatgcttt gttcatatcc ggtcacctgt
    17881 ttgggaagtt gaattaacaa acaagtaaca atgtaagccg gtccggcaaa cactctatcc
    17941 atttcctgaa gcggctatcc agaaagcgaa aaaagttgtg tctccgttac gtttctggaa
    18001 gctggctctt aaaattgccc agaagtgtgt acagctgatc tttttctttg cccggaagat
    18061 tacccatcag ctcgttcaac cattgctgat gcgccgtcgc catcagatcg aactggtgtt
    18121 tacccgccgc ggtcagtttg atccgacagg agcggcgatc gctgggatcg tacacccgca
    18181 ccgccatccc ctctttttcc aggctgtcgg caaggccggt gatattgccg ccggtcacca
    18241 gcatcagccg cgacagttca cccattttga taccgtccgg aaaacggttc agctgcgcca
    18301 gcatatcaaa gcgcggcagc gtgctgccga actgttcgcg catccgctgg cgcaggacgt
    18361 tttcaatcaa attgacgctg gaaagcagtc gaagccacag tttgacatcg cgggttcccc
    18421 cctcggggct aatggatgat acgtttgatt tcattgatat ttttcctcat ttcctcatca
    18481 cgttttcgcc aggtcatcac attttattat gtgattgttc tcacaaacgg gtgattatta
    18541 atcaagcgat attgtttaag tataaaatat tcatgtataa aataaagacc catcgctgag
    18601 ttgtgttaag taatcaccta cttccgaaag gagccatctc atggcagaaa ctaaagtaaa
    18661 ccgaatcttg aataacaata agttaaaact gggtttcttt gcagctaact gttctggcgg
    18721 tatggcggtc accaaaattg aggatcgctg gaataactcc tgggaaaata acctgaaaat
    18781 ggcgcagctc gccgacgagg ccggaatcga gtttctgctg ccgatcgccc gctggatcgg
    18841 ctacggcggc gaaaccaatt tccacggcaa cgtgctggag accgtcacct gggcttcggc
    18901 gctgctggcg gcaacccgcc acatcagcgt cttctctacc gtgcacacct cctttaacca
    18961 cccggtggtg gtggcgaagc aactggcgac catggaccat atcggccagg gccgcgcggg
    19021 gctgaacgtg gtctgcggct ggaaccgtcc ggagtatgaa gcgctgggcg gcgatcttgc
    19081 ctacgatcac gaaagccgct acgcctatgg ccaggagtgg ttcgaggtga tccgtaagct
    19141 gtggcgcgaa gagaaggcct tcaactggga cggcgatttc ttcaaactga aagacaccta
    19201 cggattgccg ttgccgctgc aaaaagatat cccgatcttc aacgccgcgg gttccggcga
    19261 gggccgcgat ttcgctattc gcaacgcgga cttcctgttt acttccgcca tcacgctgga
    19321 gcgttccgcg aaagagattg ccgagctgaa gcagaaagcc atcgacgcag cgcgccagaa
    19381 ggcgctcaat attctgacct tctcctatgt ggtttgccgc ccgacgcgcg cagaagcgga
    19441 agagtatcac cactactatt cccagcagaa cgccgattgg gcggcagtgg ataacattat
    19501 tcgtctgatg ttcgagaacg ccgaatcgtt cccgaaagat cagttgaagc tgattcgcga
    19561 tcgcttctct gccgggcacg gcggttaccc gctggtcggc accccggacg atgtcgccga
    19621 cggtctggag cggctgtacc gcaccgggtt ctccggcacc acgctgtcgt ttaccgatta
    19681 cgtgagcgag ttcccgtact tccgcgacga ggtgctgccg cgcctggaag cgcgcgggct
    19741 gcgtgagccg ttccgtccgt atgattccgc tgcaggagaa gccgcatgag ttcgctgtcc
    19801 gctgatgaat tcaaactcgg tatgcgtcaa ctgacgctgc cggtagcggt gattaccgtc
    19861 cattccgacg agcagcgcaa cggcttaacc gcctcgtcgt tctgctcggt gagcgtcgac
    19921 ccgccgcgcg ttctggtctg catcgggaaa agcgccagcg cccatcgcca gctaaacgtg
    19981 ggcgaaggta ttggcattaa cgtgctcgcc agcgaacagc tggagcacgc gttgcgtttc
    20041 gccagcaaag agtttcacgg tgagcagcgc ttcgctaaag gcaactggca cccgctgaca
    20101 tccggcgcgc caatgctgga aggcacggcg gtgagttttg actgcgtggt ggatcagatt
    20161 attgacgaca ccacccacag tatcgtcacc gccacggtag ttgccgttga gcggagcaac
    20221 aatgattgtc tgctctatcg caatggcgcg ccagttcaga caagcagcat cgcttaaaac
    20281 gcattactaa aagcctagtc gatgtattcc agaaacaatt acttatagcc catgaatttc
    20341 aggttgcagg caacacgcct gcaacctgaa agacgacggc tatttaaagg gtaaatatca
    20401 tgtcctcatc ctttaccagg ctccctcgtg gggccagggg gcttcttgcg ctcattttag
    20461 tggctagcgc ctcggcagaa gccaccacgc catccgtgac agaacccgcg ggtattaacc
    20521 tcggcctgac cagtttttat gatggcttcc cggccgccgc gccggggcaa ttgtcctatc
    20581 tgggttatta tcgttatacc catgcgaatg cgattaaaga caacgccggg aataaaatcg
    20641 atgcctttgg taaaagtaac gtcgatacct ggctcaccag ccatcaattt ctctatacct
    20701 ttgcccattc accctttgat aacgcctcgc tgggtttaga tctgattatc cctatcgtcg
    20761 gttttaatac cgatttcggc gatgccccgg cgaaattaca ggccaacgac gccgggtttg
    20821 gcgacatctt tgtcggcacc tatttgcaaa tgaaccctgt gatggataca caaggcaccc
    20881 cgctgtttgc ccagcgcttt gaaatcggtt ttaacgtgcc caccgggaaa tatgacgccc
    20941 accaggatat taacccgggc gctaatcagt ggtcgtttac gcccacttgg gccgccacct
    21001 ggttattcgc tccgcgtgcg tcgctaaact ggcgtttaca gtatctggta aacttcaata
    21061 atacccgccc tgccagcagc acaccgctgg actggaaagg cgagagtgtt gaacgcaccc
    21121 gtgccggaca gctggcctgg cttaattttg ccgccgaata tgaagtagcg ccaaaatggc
    21181 atctggggat taacggctat tatctgaagc aaatttccga cgataaagtg aatggcgatg
    21241 atctgccgaa ttcacgcgag caggtattgg ggattggccc tggtgtgatg tggaatcatt
    21301 cgcctaataa cacgctaact ctgaatttct acaccgaaac ggcagtagaa aatcggctca
    21361 gaaatagcgc tatcgtgaac ctgttttata ttcatggatt ctaggaatca gtcatgtatc
    21421 ccggccaagc gaagcactgc tgggaattca ccggagtcgg cgtaaatgcc tcgcccagac
    21481 taccttaggt agctgacgat gttgatttac agttattgta agcccggaag actggattgc
    21541 gagtcactac acgtaacact tacagtcagc gaaatgattc caaaatattg acgtatccta
    21601 agtctggagt gaatgagata gcttgctgga aataatgtgt acgttgaagt gttgtagttg
    21661 ccctgtagaa gatggtcagc acattcataa tctgcagtcg gaacaaaaaa attgaataac
    21721 gatagacgta aaaaacccga ctggtgatcg ggttttttat attccagcat tgtgtttatg
    21781 ccctggctgg atgaaacaac attcatataa caagcgagcg taaccgttgc gacagacagt
    21841 ctaaaagtaa acaacggtgt ctccctccag tttatctgga gaagcgtgat ctttccca
//
LOCUS       DAJSKJ010000055        21482 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000055 DAJSKJ010000000
VERSION     DAJSKJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.055"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            212..1255
                     /gene="nadA"
                     /locus_tag="ON345_001793"
     CDS             212..1255
                     /gene="nadA"
                     /locus_tag="ON345_001793"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR3071866.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1293..2012
                     /gene="pnuC"
                     /locus_tag="ON345_001794"
     CDS             1293..2012
                     /gene="pnuC"
                     /locus_tag="ON345_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR3071867.1"
                     /translation="MDFFSVQNILEHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="ON345_001795"
     CDS             complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="ON345_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR3071868.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3064..3444)
                     /locus_tag="ON345_001796"
     CDS             complement(3064..3444)
                     /locus_tag="ON345_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR3071869.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3760..4812
                     /gene="aroG"
                     /locus_tag="ON345_001797"
     CDS             3760..4812
                     /gene="aroG"
                     /locus_tag="ON345_001797"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR3071870.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4970..5722)
                     /gene="gpmA"
                     /locus_tag="ON345_001798"
     CDS             complement(4970..5722)
                     /gene="gpmA"
                     /locus_tag="ON345_001798"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3071871.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5924..6964)
                     /gene="galM"
                     /locus_tag="ON345_001799"
     CDS             complement(5924..6964)
                     /gene="galM"
                     /locus_tag="ON345_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR3071872.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLIDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6958..8106)
                     /gene="galK"
                     /locus_tag="ON345_001800"
     CDS             complement(6958..8106)
                     /gene="galK"
                     /locus_tag="ON345_001800"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR3071873.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8110..9156)
                     /gene="galT"
                     /locus_tag="ON345_001801"
     CDS             complement(8110..9156)
                     /gene="galT"
                     /locus_tag="ON345_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR3071874.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9166..10182)
                     /gene="galE"
                     /locus_tag="ON345_001802"
     CDS             complement(9166..10182)
                     /gene="galE"
                     /locus_tag="ON345_001802"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3071875.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10443..11915)
                     /gene="modF"
                     /locus_tag="ON345_001803"
     CDS             complement(10443..11915)
                     /gene="modF"
                     /locus_tag="ON345_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR3071876.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(11983..12771)
                     /gene="modE"
                     /locus_tag="ON345_001804"
     CDS             complement(11983..12771)
                     /gene="modE"
                     /locus_tag="ON345_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR3071877.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12900..13049
                     /gene="acrZ"
                     /locus_tag="ON345_001805"
     CDS             12900..13049
                     /gene="acrZ"
                     /locus_tag="ON345_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR3071878.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13215..13988
                     /gene="modA"
                     /locus_tag="ON345_001806"
     CDS             13215..13988
                     /gene="modA"
                     /locus_tag="ON345_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3071879.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGIITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            13988..14677
                     /gene="modB"
                     /locus_tag="ON345_001807"
     CDS             13988..14677
                     /gene="modB"
                     /locus_tag="ON345_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR3071880.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14680..15738
                     /gene="modC"
                     /locus_tag="ON345_001808"
     CDS             14680..15738
                     /gene="modC"
                     /locus_tag="ON345_001808"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR3071881.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15739..16557)
                     /gene="ybhA"
                     /locus_tag="ON345_001809"
     CDS             complement(15739..16557)
                     /gene="ybhA"
                     /locus_tag="ON345_001809"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR3071882.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16712..17707
                     /gene="pgl"
                     /locus_tag="ON345_001810"
     CDS             16712..17707
                     /gene="pgl"
                     /locus_tag="ON345_001810"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR3071883.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17748..18701)
                     /locus_tag="ON345_001811"
     CDS             complement(17748..18701)
                     /locus_tag="ON345_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3071884.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            18885..19937
                     /locus_tag="ON345_001812"
     CDS             18885..19937
                     /locus_tag="ON345_001812"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR3071885.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20013..21446
                     /locus_tag="ON345_001813"
     CDS             20013..21446
                     /locus_tag="ON345_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR3071886.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         5106 a         5285 c         5599 g         5492 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcaactatc
      121 ttgtttagca tataaaacaa attacaccga taacagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgta atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgatcccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctactttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc cgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat cgtactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatcttg atagtttggg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 tggagacatt ctatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcaga ggcttattgt ggcaaccgat cggggtattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgcagc tgcgcgcatt gtccgtggat ggccatgaat ggccttcagg ccatcgcaga
     1141 ggcattagaa caggaaggaa gcaatcacga ggttcatgtt gatgaaaggc tgcgagagag
     1201 ggcgctggtg ccgctcaatc gtatgctgga ttttgcggct acactacgtg gataacgaat
     1261 aataaggcgt aacgttacgc tttgggggaa agatggattt ttttagtgtg cagaatatcc
     1321 tggaacatat accaatagga gcaggcggtt atgatctctc atggatcgaa gcggtaggca
     1381 cgatcgccgg gttgctgtgt attggccttg ccagtctgga gaagatcagc aactacttct
     1441 ttggcctgat caacgtcacc ttgtttggca ttattttctt tcagattcag ctgtatgcca
     1501 gcctgctatt acaggtattt ttctttgccg cgaatattta cggttggtat gcgtggtcgc
     1561 gacaaaccag tcagaacgag gcggagttga aaattcgctg gttaccattg ccgaaggcgc
     1621 tcagctggtt ggcggtttgc gttgtttcga ttggtctgat gacggtattt atcaatccgg
     1681 tgtttgcatt tttgacccgc gtggcagtca tgatcatgca agcattagga ttacaggttg
     1741 tgatgcctga actgcaaccg gacgctttcc cgttctggga ttcatgcatg atggtgttat
     1801 ctatcgtggc aatgattctg atgacgcgta agtatgtgga aaactggctg ttgtgggtga
     1861 ttattaacgt gattagcgtc gttatttttg cacttcaggg cgtttacgcc atgtctctgg
     1921 agtacatcat cctgaccttt attgcgctca acggcagccg gatgtggatc aacagcgcac
     1981 gtgaaagagg ctcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg ccagacaccc
     2041 cttcattcag gtggcagtct ggcccatgac aaggttgata ttccatctga atggtggcgt
     2101 gctcaatctg atagtgatcc atcaggtagt gttggatctg atccaacaag gcatcgtgat
     2161 cgtgtggcgg gatcacctgc acatgcaacg tcatcaccgg cttctcgcct accatccata
     2221 catgtacatg gtgcacattg cggacttccg ggatttcccg gcacatacga cgcttcagtt
     2281 cagcgatatc cagcgatacc ggtgcacctt caagtaattc attcacgcta tctttcaaca
     2341 gtcgccacgc gctacgcaga accagaagcg acaccagtat cgagagaatg gggtcagcag
     2401 gtgtccagcc agtccagata ataatcaacg cggcaataat agccccaacc gaacccagca
     2461 ggtccccgag cacatgcagt gccgctgctc gtacgttgag gtttttctct tcgctgccgt
     2521 gatgaagtaa ccagaaagaa agtatatttg ccagcaaccc agccacggca attgccatca
     2581 tcatgccgcc ctcgaccgga cgcggcgtgc ggaagcgttc tatcgcctcc cagacaatca
     2641 aaatggtaat taccacgagg gctatggcat tcacaaacgc cgcgagcgtg gtcagtctta
     2701 gccaaccgaa agtgtggcga atggtgggag gacgacgtga aaattgcacg gcgagtaggg
     2761 cgaaaagcag ggcggcagta tcggtcaaca tatgacccgc atcggccagc aatgccagag
     2821 aaccagaaag aaaaccacca acgacttcta ccagcataaa cccggcagtc acgccgaaag
     2881 catacaacaa gcgacgagca ttattatctt ctggcaggtg tgaggacgtg tgtgagtgtg
     2941 agtgcgccat gacgagtcat cccttaatta ttctcactta actttatgac atcatactgc
     3001 ttttagaagt gaaaaattaa aagggagaga ctccgctctc ccattattgg ctattttgca
     3061 gggttactgc gtggtaccgt cggttttggt atcgacatca ttattgatgc catcaccggt
     3121 ttgtaccttt ttattgatat ccggacatcg accatctttg cacatggtgt tcttgtgctc
     3181 ttcatctttg gtcattccgt cattgttcat tgaagaacca tccgaatgca gcattgtgcc
     3241 gccagaaccg gtatttaccc cgttattgtc gacgttattt ggcgcgacat tttcacgggc
     3301 gtcaggggct acctggcccg catcagctgc ggcgtttgcc tggccgttat tagtttgcgc
     3361 tccgctatcg gcagccagtg cggcaccgct ggcaaggctt agagtggcag tcagaaataa
     3421 tgtggccagt tttgtcattt tcataggatg ctcctgttat ggtcgttatg tcggataacc
     3481 tcttccaaca gtgcatttgc aggtgaatat aaggcattgg tttaagattt cagccagttt
     3541 atgaaacgca gcagagaatc ttgaaataat taacaaacaa aggagttaca gttagaaatt
     3601 gtaggagaga tctcgttttt cgcgacaatc tggcgttttt cttgctaatt ccaggattaa
     3661 tccgttcata gtgtaaaacc ccgtttacac attctgacgg aagatataga ttggaagtat
     3721 tgcattcact aagataagta tggcaacact ggaacagaca tgaattatca gaacgacgat
     3781 ttacgcatca aagaaatcaa agagttactt cctcctgtcg cattgctgga aaaattcccc
     3841 gcaactgaaa atgccgcgaa tacggttgcc catgcccgaa aagcgatcca taagatcctg
     3901 aaaggtaatg atgatcgcct gttggtggtg attggcccgt gctcaattca tgatcctgtc
     3961 gcggctaaag agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa agatgagctg
     4021 gaaatcgtaa tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg gaaagggttg
     4081 attaacgatc cgcatatgga taacagcttc cagatcaacg acggtctgcg tatagcccgt
     4141 aaattgctgc ttgatattaa tgacagcggt ctgccagcgg cgggtgagtt tctcgatatg
     4201 atcactcctc agtatctcgc tgacctgatg agctggggcg caattggtgc acgtaccacc
     4261 gaatcgcagg tgcaccgcga actggcgtct ggtctttctt gtccggtagg ttttaaaaat
     4321 ggcacagacg gtacgattaa agtggctatc gatgccatta atgccgccgg tgcgccgcac
     4381 tgcttcctgt ccgtaactaa atgggggcat tcggcgattg tgaataccag cggtaacggt
     4441 gattgccata tcattctgcg cggcggtaaa gagcctaact acagcgcgaa gcacgttgct
     4501 gaagtgaaag aagggctgaa caaagcaggc ctgccagcac aggtgatgat cgatttcagc
     4561 catgctaact cgtccaaaca attcaaaaag cagatggatg tttgtgctga cgtttgccag
     4621 cagattgccg gtggcgaaaa ggccattatt ggcgtgatgg tggaaagcca tctggtggaa
     4681 ggcaatcaga gcctcgagag cggggagccg ctggcctacg gtaagagcat caccgatgcc
     4741 tgcatcggct gggaagatac cgatgctctg ttacgtcaac tggcgaatgc agtaaaagcg
     4801 cgtcgcgggt aaggtttaat tgtcggatgc gccgtcagag tggcgtatcc gatgaatcac
     4861 cacaggcctg ataagtcgcg cagcgtcgca tcaggcaatg tgctccattg ttagcaacaa
     4921 aaaagccgac tcacttgcag tcggctttct cattttaaac gaatgacgtt tacttcgctt
     4981 taccctggtt tgcaaccgcc gctgctttcg ctgcgatctc gtcagcatta cccagataat
     5041 agcgtttcag cggtttgaaa ttctcgtcga actcatacac cagcggcacg ccagtcggga
     5101 tattcagctc aagaatctct tcttcgctca tgttatcaag atatttcacc agcgcacgca
     5161 gggagttacc gtgtgcggcg atgattacgc gctcaccgct cttcatacgc ggtagaatag
     5221 tttcattcca gtaagggatc acgcggtcaa tggtcagcgc caggctttcc gtcagcggca
     5281 gttctttctc gctcagtttc gcgtaacgcg gatcgtgacc cggataacgc tcatcatctt
     5341 tagtcagttc cggcggagtc actgcaaaac cacgacgcca ctgtttcacc tgctcgtcgc
     5401 catacttttc agcagtttcc gctttgttca gaccctgcaa cgcaccgtag tgacgttcgt
     5461 tcagtttcca ggatttctca acgggcagcc atgcctgatc cagttcgtcc agcacattcc
     5521 acagggtatg gatagcgcgt ttcagcacag aagtgtaagc aaagtcaaag ctgtaacctt
     5581 cctctttcag cagcttacct gctgcttttg cttcgcttac gcctttctca gacagatcca
     5641 cgtcgtacca accggtgaaa cggttttctt tgttccactg actttcgcca tgacgaacca
     5701 gaaccagctt agttacagcc atatacttac tcctcaaatc atcttttaat gataataatt
     5761 ctcattatat tgccgcgacg aagcaacagc aatgcttacg cataaccata gcgaaaatag
     5821 tggcgcagtg taaggttgtt gtgaatattg agttgcaaat atgtcggtgt ttgctggtga
     5881 tttgaacaat atgagataaa gccctcatga cgagggctta acattactca gcaataaact
     5941 gatattccgt caggctggaa tactcttcgc caggacgcag gaagcagtcc ggttgcggcc
     6001 attcagggtg gttcgggctg tccggcagaa actcgctttc caacgccagc ccttgccagt
     6061 cggcgtaagg ttcggttccc cgcgacggtg tgccgccgag gaagttgccg gagtagaatt
     6121 gaagagccgg agcggtggtg tagaccttca gctgcaattt ttcatctgct gaccagacat
     6181 gcgccgccac tttcttgcca tcgcctttgg cctgtaacaa gaatgcgtga tcgtaacctt
     6241 tcactttgcg ctgatcgtcg tcggcaagaa actcactggc gatgattttg gcgctgcgga
     6301 aatcaaaaga cgttccggcg acagatttca agccgtcgtg cggaatgccg ccttcatcaa
     6361 ccggaagata ttcgtccgcc agaatctgca acttgtgatt gcgcacgtca gactgctcgc
     6421 cgtcaagatt gaaatagacg tgattagtca tattcaccgg gcaaggttta tcaactgtgg
     6481 cgcgataagt aatggagata cggttatcgt cgatcagacg atattgcacc gttgcgccga
     6541 gattacccgg gaagccctga tcgccatcat ctgaactcag ggcaaacagc acctgacgat
     6601 cgttctggtt cacaatctgc cagcgacgtt tgtcgaaccc ttccgggccg ccgtgcagct
     6661 ggttaacgcc ctgacttggc gaaagggtca cggtttcacc gtcaaaggta taacggctat
     6721 tggcgatgcg gttggcataa cgaccaatag aggcccccag aaacgcggcc tgatcctgat
     6781 agcattccgg gctggcacag ccgagcagcg cctcgcggac gctgccatcg gaaagcggaa
     6841 tacgggctga aagtaaagtc gcaccccagt ccatcagcgt gactaccatc cctgcgttgt
     6901 tacgcaaagt taacagtcgg tacggctgac catcgggtgc cagtgcggga gtttcgttca
     6961 gcactgtcct gctccttgtg atggtttaca aacgtaaaaa gtctctttaa tacctgtttt
     7021 tgcttcatat tgttcagcga cagcttgctg tacggcaggc accagctctt ccgggatcaa
     7081 cgcgacgata cagccgccaa atccgccgcc ggtcatgcgt acgccacctt tgtcgccaat
     7141 cacagctttg acgatttcta ccagagtgtc aatttgcggc acggtgattt cgaaatcatc
     7201 gcgcatagag gcatgagact ccgccatcaa ctcgcccata cgtttcaggt cgccctgctc
     7261 cagcgcgctg gcagcttcaa cggtgcgggc gttttcagtc aggatatgcc gcacgcgttt
     7321 cgccacgatt gggtccagct catgtgcaac agcgttgaac tcttcaatgg tgacatcgcg
     7381 cagggctggc tgctggaaga aacgcgcacc ggtttcgcac tgttcacgac gggtgttgta
     7441 ttcgctgcca accagggtac gtttgaagtt actgttgatg atgacgacag ccacaccttt
     7501 cggcatggaa actgctttgg tccccagtga acggcagtca atcagcaagg catgatcttt
     7561 cttgccgagc gcggaaatta gctgatccat gatcccgcag ttacagccaa caaactggtt
     7621 ttctgcttcc tgaccgttaa gcgcgatttg tgcgccgtcc agcggcagat gataaagctg
     7681 ctgcaatacg gttccgaccg cgacttccag tgaggcggaa gaacttaacc cggcaccctg
     7741 cggcacattg ccgctgatca ccatgtccac gccgccgaag ctgttgttac gcagttgcag
     7801 atgtttcacc acgccacgaa cgtagttagc ccattgatag ttttcatgtg cgacaatggg
     7861 cgcatcgagg gaaaactcgt cgagctgatt ttcataatcg gctgccatca cgcgaacttt
     7921 acggtcatcg cgtggtgcac aactgatcac ggtttgataa tcaatcgcgc agggcagaac
     7981 gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc acgcggccag gcgcctgaat
     8041 ggtgtgagtg gcagggtagc caaatgcgtt ggcaaacaga gattgtgttt tttctttcag
     8101 actcatttct tacactccgg attcgcgaaa atggatatcg ctgactgcgc gcaaacgctc
     8161 tgctgcctgt tctgcggtca ggtctcgctg ggtctctgcc agcatttcat aaccaaccat
     8221 aaatttacgt acggtggcgg agcgcaacag aggcggataa aagtgcgcgt gcagctgcca
     8281 gtgttgattc tcttcgccat taaatggtgc gccgtgccag cccatagagt aggggaagga
     8341 gcactggaag aggttgtcat aacgactggt cagctttttc aacgccagcg ccaaatcgct
     8401 gcgctgggcg tcggtcaaat cggtgatccg caaaacgtgg gctttgggca gcagtagcgt
     8461 ttcgaatggc caggcagccc agtaaggcac aacggctaac cagtgttcgg tttcgacaac
     8521 ggtacggcta ccgtctgcca gctcgcgctg aacataatcc accagcattg gtgatttctg
     8581 ttcggcaaaa tattcttttt gcaggcggtc ttcgcgctca gcttcgttag gcaggaagct
     8641 atttgcccaa atctgtccgt gcggatgcgg gttagagcag cccatcgccg cacctttgtt
     8701 ttcaaagacc tgcacccacg ggtacgtttt tcccagctct gcggtttgct cctgccaggt
     8761 tttgacgatt tccgtcaatg ccgcaacgct cagttctggc aacgttttac tgtgatccgg
     8821 tgaaaagcag atcacccggc tggtaccgcg cgcgctctgg caacgcatca aaggatcgtt
     8881 gctttctggc gcatctggcg tgtcagacat caaggccgca aagtcattag tgaaaacgta
     8941 agtcccggta taatcggggt ttttatcgcc tgtcacccgc acattacctg cgcagaggaa
     9001 gcaatctgga tcgtgcgcag gtaacacctg tttggctggc gtttcctgcg ccccctgcca
     9061 ggggcgctta gcgcggtgcg gtgaaaccag aatccattgc ccggtgagcg ggttgtagcg
     9121 gcgatgtgga tgatcaacgg gattaaattg cgtcatggtc gttccttaat cgggatatcc
     9181 ctgtggatgg cgtgactgcc agtgccaggt gtcctgcgcc atttcatcga gtgtgcgcgt
     9241 tacgcgccag ttcagttcac ggtcggcttt gctggcgtcc gcccagtagg ccggaaggtc
     9301 gccctcgcga cgcggtgcaa aatgataatt aaccggtttg ccgcaggctt tgctgaaggc
     9361 attaaccacg tccagcacgc tgttgcctac gccagcgccg aggttgtaga tgtgtacgcc
     9421 tggcttgttc gccagttttt ccatcgcgac gacgtgaccg tccgccagat ccattacgtg
     9481 gatgtaatcg cgtacgccag taccatcttc ggtcggataa tcgttaccaa aaatcgccag
     9541 cgagtcgcga cggcctacag caacctgggc gatgtatggc atcaggttat tcggaatgcc
     9601 ttgcggatct tcgcccatat cgcccgacgg atgcgcgcca accgggttga agtagcgcag
     9661 cagggcaatg ctccagtccg gctgggcttt ttgcagatcg gtgaggatct gttccaccat
     9721 cagcttgctt ttgccgtaag ggctttgcgg tgtgccggtc gggaagcttt caacgtatgg
     9781 aattttgggc tgatcgccat aaacggtggc ggaggagcta aaaataaagt ttttgacgtt
     9841 agcggcgcgc atggcgctaa tcaggcgcag agtgccgttg acattgttgt cgtaatattc
     9901 cagcggtttt tgtaccgatt cgccaacggc tttcagcccg gcgaagtgga tcacggtgtc
     9961 gatagcgtga tcgtgcagta tctcggtcat caacgcttcg ttacgaatat cgccttcaac
    10021 aaacgttgga tgtttgccgc ctaaacgctc gataacaggc agtacgctgc gcttactgtt
    10081 acagaggtta tcaagaatga tgacatcatg accgttttgc agtaattgca cgcaggtatg
    10141 acttccaatg taaccgctac caccggtaac cagaactctc ataattcgct ccattaggct
    10201 tatggtatga aataaccata gcataacaaa gatgcgaaaa gtgtgacatg gaataaatta
    10261 gtggaatcgt ttacacaaga atttagccgt tttttatgcg tgattaagtg attataaaac
    10321 agagggttta tgaatgattg cgctttttat ctgaaaaaag aagcggtttc atgcctgcat
    10381 gcgtcgaacc gttggccgga gagggcgctt aggccgcctc cggcaaggtc agcactaccg
    10441 actcaatata tttttgtcag cacatagcga tagagtccac cgtccggcac gaactcaagg
    10501 cgatgggtaa tacaggcagg cgcatcttca gcgtggtgcg aaacaaacaa caattgcgtt
    10561 tcaccttcgc taatcagcac atcaacaaaa cggcggataa gctggcgatt gagcggatca
    10621 agcccctgta gtggttcatc gagaataagc aacgtcggat gtttcaccag tgcgcggacg
    10681 atcagcgcca gacgctgctg tccccaggaa agactatgga acggagcgtc agccgtacgt
    10741 ttatcaatgc cgagaatatc cagccactgc tgcaccagtt tttgctggcg atccgaaacg
    10801 gcctgataaa tgccaatcga atcaaaatag ccagaaagaa tcacattacg cacagtagta
    10861 ctgacccggt aatccagatg caaactactg ctgacgtaac cgatatgctt tttgatatcc
    10921 cagatggttt cgccgctgcc gcgacgtcgt ccgaaaagcg tcaaatcgtt gctgtaacct
    10981 tgcggatgat cgccagtaac caggcttaat aacgtcgatt ttcctgcacc atttggcccg
    11041 acaatttgcc agtgttcgcc tggattcacc tgccagctaa ggttattaag aatggggcga
    11101 tcgttataag aaaccacgcc attgttcagc acaatgcgcg gttcgttggc gggtaaggcg
    11161 tgacgtgctg aaggttcatc cggctccggc agttgcacac cttcaagctg ttcactatgc
    11221 gccagttgcg cgacgagtgc ttgttggagc agttcctctt tagcgccagt ttccgctaac
    11281 gtgcaatccg ccagcacgcc agcaaactgg acaaactccg ggatctcatc gaagcgattg
    11341 agcaccagta ccagagtaat accggactga tgtaacgagg cgagtcgctc agccagctgc
    11401 tgacgtgagg caacatccag gccatcgaac ggctcatcaa gaatcaacaa gtcaggctcc
    11461 gacatcagcg cctgacacag cagggttttt cgcgtctcgc cagtggaaag gtatttaaag
    11521 cgtcggtcga ggagggcggt aataccgaac tgctgcgcca gttgcatgca acgcggtgca
    11581 tcctttactt catcctgaat gatctcagcc gtagtgcgtc cggtgtcatc ttcgccaggg
    11641 ccgagcatat cggtgttatt ccgctgccat tcgtcgctga cgagtttttg caattgctcg
    11701 aaggagagac gagtgatgtg ggaaaactgg ctttgccgtt cacctttcaa aagcggaagt
    11761 tcccccgcca gcgcgcgggc cagggccgat ttcccgcttc cattcgaacc gacaaacgcc
    11821 caactatcac ccgcgtttaa cgttagctga ggcaattgca gcgtttttgt gtcgctaaga
    11881 cgaaatgtgc cttgcaaaat ttgcaacgat gacattttat atcccatttt gtgcagcgat
    11941 tactgacagg gatacgtgtt tcataacaaa ttgtcaacac gcttagcaca gcgtggcgat
    12001 aatcacgctg tcggcattaa agtaggccgt gacattctgc ccttgctgaa gagaagtcgc
    12061 ttcatttacc ggcactgtgg cgcacagtgt ttgcccgtcg ggtagcgcca ttaatacttc
    12121 gcactgctct gcgccgcgct caatatgact aataataccc ggtaattggt tgtcagcgtt
    12181 ttgcgcgacc gcctcgtcct gagtaatacc tacccacggc gcttttagca atatcaacac
    12241 ttctttgcct tcatccagcc ccagacgcgc gccgctttgt gcggtaattg cgactttcag
    12301 gcgtgttttt ccgtcagcca gtaagacatc aacatgctgt tgaacgtcat catgatcgcg
    12361 ggcggtgatg gtgccgaacc actggttacg ggcgctggtt tgcagtgaaa aacgtgagat
    12421 cgcggccagc aggctgttca gcggcagggc gtcatcgtca cttaacacat caaaggcttt
    12481 ttgctggatt tgcgccagta agtcatagag ctgaatcagt cgctgaccat agcgggtcag
    12541 tactgcgccg ccgccacctt taccgcctgt tgcgcgctcg accagaatat gctcacttaa
    12601 ctgattcatc tcgttaatgg catcccaggc gcttttatag ctgataccgg catctttcgc
    12661 tccctggcta atggaaccgg aaagcgcaat gtgttttagt agcgaaatcc ggcgcgggtc
    12721 ggcgaataat ttttgttgga gcttaagggt gagaaggatt tcggcctgca taacaatgtc
    12781 ctggcaaaag tcttattgtg acggaaaacg aacgcaacgc aaagctgacc gcacaaaagg
    12841 ggagtgcttt tctgtgctta gcggttagaa tagtctcatg actatatctg gagttgacca
    12901 tgttagagtt attaaaaagt ctggtattcg ccgtaatcat ggtacctgtc gtgatggcca
    12961 tcatcctggg tctgatttac ggtcttggtg aagtattcaa catcttttct ggtgttggta
    13021 aaaaagacca gcccggacaa aatcattgat tccctgaatg cccgcttagt cgggcatttc
    13081 tttttctcaa cttcctgctt ttccagccga tattttttct tatctacctc acaaaggtta
    13141 gcaataactg ctgggaaaat tctgagctag ccgttatatt gtcacctata taacgttaca
    13201 ttaaggggtt accaatggct cgtaaatggt tgaacttgtt tgccggggcg gcactctctt
    13261 tcgctgttgc tggcaataca ctggcagatg aagggataat cacggtgttc gccgccgcat
    13321 cactgactaa cgcaatgcag gacattgcta cgcagtataa aaaagaaaaa ggcgtggatg
    13381 tggtttcttc tttcgcttcg tcatctactt tggcccgtca gattgaagcg ggtgcgcctg
    13441 cggatctgtt tatttctgcc gatcagaaat ggatggatta tgcggttgat aaaaaagcga
    13501 tcgatacggc ttcgcgccag acactgctcg gcaatagcct ggtcgttgta gcaccgaaag
    13561 ccagcgagca gaaagatttc accatcgaca gcaaaaccaa ctggacttcg ctgctgaatg
    13621 gcggtcgcct cgcggttggc gatccggaac atgtccctgc tggcatttat gcgaaagaag
    13681 cactgcaaaa actgggcgca tgggatacgc tctccccgaa actggctccg gcggaagatg
    13741 ttcgtggcgc gctggcgttg gtcgaacgca atgaagcgcc gttgggcatt gtttacggtt
    13801 ctgacgcagt tgccagcaaa ggggtaaaag tggttgccac cttcccggaa gattcacata
    13861 aaaaagtgga atatccggtt gctgttgtgg aagggcataa caatgcgaca gtgaaagcat
    13921 tttatgatta tttgaaaggg ccgcaggctg cggaaatctt taaacgttac ggatttacaa
    13981 ccaagtaatg atactgaccg atccagaatg gcaggcagtt ttattaagcc tgaaagtttc
    14041 ttccctggct gtgctgttta gcctgccgtt tgggatcttt tttgcctggt tactggtgcg
    14101 ttgcacgttt ccgggcaaag ctctgctcga cagcgtactg catctaccgc tggtgttacc
    14161 gcccgtggtc gtcggttact tattattagt ttcgatggga cggcgcggat ttatcggtga
    14221 acgtctgtat gactggtttg gtattacctt cgcctttagc tggcgcggcg cggttctcgc
    14281 tgccgccgtc atgtcgtttc cgctgatggt gcgggcaatt cgtctggcgc tggaaggggt
    14341 tgatgtcaaa ctcgaacagg ccgcaagaac actgggggcc gggcgctggc gcgttttctt
    14401 tactatcacg ttaccgctga ccttaccggg aattattgtt ggtacggtac tggcttttgc
    14461 tcgttctctc ggtgagtttg gtgcaaccat cacctttgtg tcgaacattc ctggtgaaac
    14521 gcgaaccatt ccttctgcca tgtataccct gatccagacc cccggcgggg aaagtggcgc
    14581 ggcgagactg tgcattattt ctattgcgct ggcgatgatc tccctgttga tttcagaatg
    14641 gctggccaga atcagccgtg aacgggcggg gcgctaatca tgctggaact gaatttttcc
    14701 cagaccttgg gcaaccattg cctgaccatt aatgaaacgc tgcccgccaa tggcatcact
    14761 gctatctttg gcgtctccgg tgccggaaaa acttcgctga ttaacgccat cagtggactg
    14821 acgcgcccgc aaaaagggag gattgtcctc aatggtcggg tactaaatga tgccgaaaaa
    14881 ggtatctgcc tgacgccaga aaagcgtcgc gttggctatg tttttcagga tgcgcggctg
    14941 tttccgcatt acaaagtgcg tggcaatctg cgctacggca tggcgaaaag catggtcgat
    15001 cagttcgata agctggtggc gcttttaggc attgaaccgt tgcttgaccg tttaccaggc
    15061 agcctgtccg gaggcgaaaa acagcgcgta gcgattgggc gggctttgct gacggcacca
    15121 gaattgctgt tgctggatga accgctggcg tcactggata ttccgcgtaa acgcgaactg
    15181 ttgccttatc tgcaacgtct gacgcgggaa atcaacatcc cgatgttgta tgtcagccat
    15241 tcgctggatg agatcctcca tctggcagac agagtgatgg tactggaaaa cggtcaggtg
    15301 aaagcctttg gcgcactgga agaagtctgg ggtagtagcg tgatgaatcc gtggctgccg
    15361 aaagagcaac aaagtagcat tctgaaagtg acggtacttg agcatcatcc gcattacgcg
    15421 atgactgcgc tggcgctggg cgatcagcat ttgtgggtca ataagctgga cgaaccgctg
    15481 caagctgcgc tacgtatccg cattcaggct tccgatgttt ctctggtttt acaaccgccg
    15541 cagcaaacca gcattcgtaa cgtattgcgg gcaaaagttg ttaatagtta tgacgacaac
    15601 ggccaggtgg aagtggaact ggaagtcgga ggtaaaacgc tgtgggcgcg tatcagcccg
    15661 tgggccaggg atgaactggt gatcaaacct ggcctgtggc tgtacgcgca aattaaaagt
    15721 gtgtcgataa ccgcctgatt aaatcaggtg gctataaatg aactgggcaa tgctgtcggt
    15781 ggtgttatca ccaatcacaa tgttggcgcg cgcttttacc gcgtcatcgg cgttgcccat
    15841 cgccacgcct gtaccagcgg cctccagcat actgatatca ttaaagttat cgccgaacgc
    15901 cacgacgttt tccatcgacc agccttgcgc ctcaacccat ttcgtcaaac gtttaccttt
    15961 gctgttgccg ccgcgtgcaa tatcaacctg atcgtgccag gaccattcac actccagtcc
    16021 cagttcatgt tcgacatgct taccaaaatg ctgcaattgc ggcaggtcat cgtgcgtcag
    16081 ggcgaacttc catacggcgt taacttgttg cgccgtttca gccagagaag cgacttgtgt
    16141 gaaagtcgga cgctgttccg gcggcagggt ttgcgcccag ttagacgtgc gaatgacatg
    16201 cccggtcggg tgctcataga ccattgcatc atccacatac atcagaccgt gaatgtggtg
    16261 ttcattcagc atctcaatga gttgcagggc tttattaacg ggcattgggt ccgcttccag
    16321 cacggttttt gcatgataat catacaaata ggtgccatta cagcaaatag caggtgtatc
    16381 cagcgccagc gcctgataaa aaggatgaat agcgacgtga tggcgacctg tgacgatgat
    16441 taattgatag cctgcttcgc gagcgcgggc cagggcttct atcgatgaag gaagcagggt
    16501 ctttttcggg gtcaataagg tgccgtctaa gtcgagagca atcacgcgtg tggtcatggc
    16561 gtattccaga ttaaggttaa gaattttcgt ctgcgcgaat ggtacaccga taccactatc
    16621 ggacaaaatt ctgcgtttta attcagcatt caccgccaaa agcgactaat tttagctgtt
    16681 acagtcagtt gctaaatgca aaggagcatt catgaagcaa acagtttata tcgccagccc
    16741 tgagagccag caaattcacg tctggaacct gaatcatgaa ggcgcactga cgctgacaca
    16801 ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc agcccggaca aacgttatct
    16861 ctatgttggt gttcgccctg agtttcgcgt cctggcgtat cgtattgccc cggacgatgg
    16921 cgcactgacc tttgccgcag agtctgcgct gccgggtagt ccgacgcata tttccaccga
    16981 tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga gcgtaacgcg
    17041 tctggaagat ggcctgccag tgggcgtcgt cgatgtggtc gaggggctgg acggttgcca
    17101 ttccgccaat atctcaccgg acaaccgtac gctgtgggtt ccggcattaa agcaggatcg
    17161 catttgcctg tttacggtca gcgatgatgg tcatctcgtg gcgcaggacc ctgcggaagt
    17221 gaccaccgtt gaaggggccg gcccgcgtca tatggtattc catccaaacg aacaatatgc
    17281 gtattgcgtc aatgagttaa acagctcagt ggatgtctgg gaactgaaag atccgcacgg
    17341 taatatcgaa tgtgtccaga cgctggatat gatgccggaa aacttctccg acacccgttg
    17401 ggcggctgat attcatatca ccccggatgg tcgccattta tacgcctgtg accgtaccgc
    17461 cagcctgatt accgttttca gcgtttcgga agatggcagc gtgttgagta aagaaggctt
    17521 ccagccaacg gaaacccagc cgcgcggctt caatgttgat cacagcggca agtatctgat
    17581 tgccgccggg caaaaatctc accacatctc ggtatacgaa attgttggcg agcagggact
    17641 actgcatgaa aaaggccgtt atgcggtcgg gcagggacca atgtgggtgg tggttaacgc
    17701 acactaaccg ctgatttaac cggcgcagtc tctcctgcgc cggtgtatta acttatctcc
    17761 tgtaacgcat gtctctggcg ttcgacgata ttggtccaca aattgtcttt atcgtcagtc
    17821 cacaaattaa tcagcaaggc aaaaaagcgt tcagctgccg gagaaagcac ggcatctttg
    17881 cggcgaataa tccccaatgt ccgacgtata acgggttcca ccagtgggat accaataatc
    17941 gaagaatagg gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc cagtcctgcc
    18001 tccactagtc ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc cagcttccag
    18061 ggcttgtcgg ctagctgttg ttctatcaac aatcggttgc cgctggagga acgtacgcca
    18121 atcattttgt aacccactaa ctcctgccat tctacaagct gctttttggc caatggatgg
    18181 tcacgccgac aggccagcac gaatggctcg ttgaccagtg gggtaaaatc aatggatgaa
    18241 tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt tacacagcac cgattccatg
    18301 caattattcg tgccttgttc cagaatccgc actttaatat tgggatacag ctcattaaat
    18361 ttaccgattg ccagcggtaa aaaataaaat actgcggtcg gaatacacgc taacgtcacc
    18421 ataccacgat gataagcatt catatcacga atattaaaaa gcgtttcatc gaatttcttt
    18481 attaattccc gcgcctccgg gagcaacctt tttcctgctt ttgttaacgt aaccttgcga
    18541 gttgtacgct caaatagttg aatatgtaaa tcttcctcca tcttttttat tcttcgcgtt
    18601 aatgcaggtt gcgtaatatt gagtaattta gcggcattgt taaatgaact ggactctgcc
    18661 agtatgacaa atgccttcat acttgataat tcatgcttca ttattactcc ggaaaatgga
    18721 agcgacgatt ttgggtggct ggccgttaaa aattttaact gcatttagcc aacttaaatt
    18781 aatgaaaaaa tgttattaat agttgagcta aagtcattag agatgctttg cccttaatgt
    18841 aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa aaaataccct
    18901 gcgtgatgat gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa catttacccg
    18961 aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt aacgatctgg
    19021 aaattgacgg tattggcggc ggtaatccgc tgaccagtaa agtcgccatt attagccgtt
    19081 ccagcgatcc gcgtgctgat gtcgattatc tgtttgctca agttattgtc catgagcaac
    19141 gtgtcgatac cacgcctaat tgtggcaata tgctgtctgg tgttggggca ttcgccattg
    19201 aaaatggttt gattgcagcg acttcaccag ttacccgcgt acgtatccgc aacgtcaata
    19261 ctggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag tacgagggta
    19321 gcgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact ttcctgaatg
    19381 ccgccggaac caaaaccgga aaagtcttcc cgactgataa tcagattgat tattttgacg
    19441 atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg gctgaatatc
    19501 tgggaaaaac aggttatgaa ttaccggcgg aactggatgc cgacaaagca ttattagcac
    19561 gcattgaatc tatccgtcta caagcgggta aagcaatggg cttaggtgat gtcagtaata
    19621 tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca attaatgtac
    19681 gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct attgctattt
    19741 ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct gtaggatacg
    19801 gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt aatgaaggtc
    19861 aggatgccac gacgttacgc gcatctgtta ttcggacgac cagaaaaata ttttccggtg
    19921 aagtttatct tccctgaaaa aattcgttgt caggataagg acaatcaata aaggacttct
    19981 gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta tggaagctaa
    20041 ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca ggattaagcg
    20101 aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtggggctg gttatcaagc
    20161 ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctccatggtg gtggtcggta
    20221 acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct tcaggtacta
    20281 cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc ggtttaggta
    20341 aacgtattgc ctatctgctg attggtaaaa tcggtaacac cacgctgggt ctgggttacg
    20401 ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc gcgcgtgcgg
    20461 gcggtattgt gttaccgatc atcaacagcg tggcggtggc tttggggtcc gaaccggaaa
    20521 aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc accaaaacca
    20581 ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa atgatcaacg
    20641 acattctgca cctgcaaatt agctggggtg gatgggcact ggccgccgga ttgccgggca
    20701 tcattatgct gctggtcacc ccactggtga tttataccat gtatccgcca gaaattaaga
    20761 aggtggataa taaaaccatc gctaaagcgg gccttgccga actgggaccg atgaaaatcc
    20821 gcgaaaaaat gctgctcggt gtctttgtgc tggcgctgct gggctggatt ttcagtaagt
    20881 ctctgggggt tgatgaatcc accgtggcaa tcgttgttat ggcgaccatg ctgctgctgg
    20941 gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc ttaatctggt
    21001 acggcggtat tatcggatta agctccttat tatcgaaagt taaattcttc gaatggttag
    21061 ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct ttcttcgtta
    21121 ttattttcct cagcattatc gtgcgttatt tcttcgcttc cggtagtgcc tatatcgttg
    21181 ctatgttacc ggtatttgcc atgctggcga acgtctccgg cgcaccgtta atgttaaccg
    21241 cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat ggcggcgcgg
    21301 caggtccggt catctttggc gtgggttaca acgatattaa atcctggtgg ttggtcggtg
    21361 cggtactgac gatattaacc ttcctggtgc atatcaccct cggcgtgtgg tggtggaata
    21421 tgctgatcgg ctggaacatg ctgtaaatat actcgtcata cttcaagttg catgtgctgc
    21481 gt
//
LOCUS       DAJSKJ010000056        21167 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000056 DAJSKJ010000000
VERSION     DAJSKJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.056"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            19..726
                     /locus_tag="ON345_001814"
                     /pseudo
     CDS             19..726
                     /locus_tag="ON345_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            723..2174
                     /gene="djlC"
                     /locus_tag="ON345_001815"
     CDS             723..2174
                     /gene="djlC"
                     /locus_tag="ON345_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR3071887.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPSESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2234..3904)
                     /gene="hscC"
                     /locus_tag="ON345_001816"
     CDS             complement(2234..3904)
                     /gene="hscC"
                     /locus_tag="ON345_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR3071888.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3988..4923)
                     /gene="rihA"
                     /locus_tag="ON345_001817"
     CDS             complement(3988..4923)
                     /gene="rihA"
                     /locus_tag="ON345_001817"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR3071889.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5041..5766)
                     /gene="gltL"
                     /locus_tag="ON345_001818"
     CDS             complement(5041..5766)
                     /gene="gltL"
                     /locus_tag="ON345_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR3071890.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5766..6440)
                     /gene="gltK"
                     /locus_tag="ON345_001819"
     CDS             complement(5766..6440)
                     /gene="gltK"
                     /locus_tag="ON345_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR3071891.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6440..7180)
                     /gene="gltJ"
                     /locus_tag="ON345_001820"
     CDS             complement(6440..7180)
                     /gene="gltJ"
                     /locus_tag="ON345_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR3071892.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7350..8258)
                     /gene="gltI"
                     /locus_tag="ON345_001821"
     CDS             complement(7350..8258)
                     /gene="gltI"
                     /locus_tag="ON345_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR3071893.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVSKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8713..10590
                     /locus_tag="ON345_001822"
     CDS             8713..10590
                     /locus_tag="ON345_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3071894.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAALTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10668..12206)
                     /gene="lnt"
                     /locus_tag="ON345_001823"
     CDS             complement(10668..12206)
                     /gene="lnt"
                     /locus_tag="ON345_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR3071895.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12231..13109)
                     /gene="corC"
                     /locus_tag="ON345_001824"
     CDS             complement(12231..13109)
                     /gene="corC"
                     /locus_tag="ON345_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR3071896.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13199..13666)
                     /gene="ybeY"
                     /locus_tag="ON345_001825"
     CDS             complement(13199..13666)
                     /gene="ybeY"
                     /locus_tag="ON345_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR3071897.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFSFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13663..14703)
                     /locus_tag="ON345_001826"
     CDS             complement(13663..14703)
                     /locus_tag="ON345_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR3071898.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14856..16280)
                     /gene="miaB"
                     /locus_tag="ON345_001827"
     CDS             complement(14856..16280)
                     /gene="miaB"
                     /locus_tag="ON345_001827"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR3071899.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16426..17601
                     /gene="ubiF"
                     /locus_tag="ON345_001828"
     CDS             16426..17601
                     /gene="ubiF"
                     /locus_tag="ON345_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR3071900.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGNSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARNYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17755..17829)
                     /locus_tag="ON345_001829"
     tRNA            complement(17755..17829)
                     /locus_tag="ON345_001829"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17795..17797),aa:Gln,seq:ctg)
     gene            complement(17878..17954)
                     /locus_tag="ON345_001830"
     tRNA            complement(17878..17954)
                     /locus_tag="ON345_001830"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17918..17920),aa:Met,seq:cat)
     gene            complement(17970..18044)
                     /locus_tag="ON345_001831"
     tRNA            complement(17970..18044)
                     /locus_tag="ON345_001831"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18010..18012),aa:Gln,seq:ttg)
     gene            complement(18068..18152)
                     /locus_tag="ON345_001832"
     tRNA            complement(18068..18152)
                     /locus_tag="ON345_001832"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18116..18118),aa:Leu,seq:tag)
     gene            complement(18161..18237)
                     /locus_tag="ON345_001833"
     tRNA            complement(18161..18237)
                     /locus_tag="ON345_001833"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18201..18203),aa:Met,seq:cat)
     gene            complement(18617..20281)
                     /gene="asnB"
                     /locus_tag="ON345_001834"
     CDS             complement(18617..20281)
                     /gene="asnB"
                     /locus_tag="ON345_001834"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR3071901.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(20678..>21167)
                     /gene="nagD"
                     /locus_tag="ON345_001835"
     CDS             complement(20678..>21167)
                     /gene="nagD"
                     /locus_tag="ON345_001835"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR3071902.1"
                     /translation="VVGEGALIHELYKAGFTITDVNPDFVIVGETRSYNWDMMHKAAY
                     FVANGARFIATNPDTHGRGFYPACGALCAGIEKISGRKPFYVGKPSPWIIRAALNKMQ
                     AHSEETVIVGDNLRTDILAGFQAGLETILVLSGVSSLDDIDSMPFRPSWIYPSVAEID
                     VI"
BASE COUNT         5155 a         5321 c         5470 g         5221 t
ORIGIN      
        1 ttaagggatt attaaaaaat gaataaggaa gaacaatatc ttctttttgc gctttctgcg
       61 cccatggaaa tacttaatca gagttgcaaa cccgcgcatg actcgccaaa aatgtatacc
      121 gggataaaag aattcgatct ctcatcatcc tggggaataa ataatcgtga tgatttaatt
      181 cagactattt atcagatgac tgatgatggt catgccaatg atttagctgg cttatatctg
      241 acatggcatc gctcttcccc tgaagagtgg aaagcattaa ttgctggtgg ctcggagaga
      301 gggttgattt atacccaatt cgttgcccaa accgctatgt gctgtggaga agggggaatc
      361 aaagcctggg attatgttcg aatggggttt ctgtcacggg tcggtgtgct caacaactgg
      421 ctgacggaag aagagagttt atagctgcaa tcgcgcgtct atgtacgagc tcaccattac
      481 tatcacagct ggatgcacta tttttccgcc tattcgttag gacgtctcta ctggcaatct
      541 tctcagtgcg aagacaacac ttcgctacgc gaggcgctaa ccctgtacaa atacgatagc
      601 gccgggagtc ggatgttcga agagctggca gcgggaagcg atcgtttcta tgccacgctt
      661 ccctggcagc cattaactgt ccagcctgaa tgcccggtaa cgcttaagga tgtgagcgac
      721 ctatgaaaac atgttggcaa atcctcgaaa ttgaaagcac gacgcaaata gacattatcc
      781 gccaggctta tcttgctcgc ttaccgttat gtcatcccga aaccgatccg caggggttta
      841 aagcattacg ccaggcctat gaagaggcgc tgcgactggc ggtaaatcct gtcgaggaag
      901 cagatgatga agaaaaagat gccgccgctg aacatgaaat actgcgtgca ttcaggacat
      961 tactggattc agaaagtgat cgttttcagc cctccgcctg gcagaaattt attcagcaat
     1021 taaatacctg gaacatggag gatgtcgatc aattacgctg gccgctgtgt gcaatcgcta
     1081 tagaagcgcg atatctttca ttaaattgtg cttctttgct ggcagagcgt ttgaactggc
     1141 attcgtttaa tgacagcgaa ggaatggatg aggaagaaag ggaggctttt cttgaggcca
     1201 ttcaggctgg tgattgtttc gatttcctta gccttctgga atatcccgtt gcgttgcaga
     1261 accagactgt tgagtattac ttcgcgctgg aacgttgctg ccgttaccat cctgactacg
     1321 tcactgcgtt tttggcgatg gaaggtccgt ggttcattcc tgatgatgca aagttacatc
     1381 gcaaactgtt gcgctggtac agctcggtgc aaacaggtat ggcggaactc attcctgtag
     1441 ccaaacagtg gcaaatggaa gaaccagaaa gcgaagatgc ccgatattac ttgtgtgctc
     1501 aaaggttgta ctgcggcgag ggggaaagcc tgcttgccga tctctgcgcg taccgggaaa
     1561 gttacccatc tacacaagct gataatttgt tgttgcagtg gagcaagcgt cattgcccgg
     1621 attatttcgc gttattagtg atggttatcg aagcgcggag catggtagat gcgcaaggtc
     1681 aaccgctgaa atatgttcct agtgagagcg cccggacgcg gctgttatgg gcggaaattt
     1741 tacatagcgg aaaattatcg ccgttaggtc aatcgtttat tgagtcgtta ttcttcaaac
     1801 gcaaagcatg ggcgtggtgg aaatcgagag tcggtagcga gacagaagaa gattcaccgt
     1861 tactggattt gtatcgggta gcggaacagg tagtacttga agcgtttccg aaacaagaga
     1921 tgctggcccg tcttaataca aggctggaag gcggagatgc tcatccatta gaggccatta
     1981 tcacccggat gctgttgacg aaagtaaaac tcgagccgga ggatgaagat gtcgatgagc
     2041 caacacctga aaaccatgaa gaaaaaaatg atgagggtga aaaaccacag agcattacca
     2101 gcattatcaa aatcagttta acggtgctgg ttataggtta tgctctcggc aaaatcgcga
     2161 tgttgtttag ttgacgatgt gttttttgcg ccgatgccgg atgcagcatt tacgccgcat
     2221 ccggcagccg tgattatgag gtctcaatgg ctaaataaac gctcgcccgc cgcctgacct
     2281 catcgacgcg ggccatatcg tttgactgca acgccgcatc aaaatcggtg atcacccggc
     2341 caatctcttc tcgctcgtca cccagcgccc gcgcccacaa ctcttccagt ttggccttaa
     2401 aggtgcggtt gatgagcata tcgcgcggat aaattttcaa tgcggataac cgcgtccgac
     2461 tctcttcaat ctgctgcgcg ctcaatgtta ccgggctgtg gttaatcact ctggacttaa
     2521 cgctgccgtc ttccagaagc acgtcaactt caagcaaccc gttgatatca taactaaagc
     2581 gaatatcaat cgactgataa gccccggttt tcttcaacgg cacatcgaag gattccacca
     2641 gaatgttgtt tttaactttg tggttttctc cctgatagac gttaaccgtg attgaatcct
     2701 gttccgggtg catggttgaa taagtttcta cacgcgacac gggcacagtg gtgtttcgtt
     2761 caataatcgg cgagaaaatg ccggaaacgc cctggcggtt aacttcaacg cccaacgagt
     2821 aagggcaaat atcagtgagg atcacctctt caatatcttc actgcgtaag cggcaggcgg
     2881 cctggattgc tgcgcccagc gcgacaatgg tgctcggatc gtaactttga tacggtaatt
     2941 tgccaaacag acgcacggcg attcgctgca ccagcggcat ttgtgacgcg ccgccaacca
     3001 gcaccagact gtcgatttga ctcggcttca gacgcgcatc gcgcaacgcc tgttcaatcg
     3061 gcactcgcaa gcgattgagc agcggcaacc acaaatcttc cagttcgttc tcgtaaaatt
     3121 cgcattcccg cgtttcttcc tgatactgcc agcgaatgtg caatggcgat tgattgctac
     3181 attttgccgc ttccacacag gcgtacaagg ctgccagttc actctcgtta agcgtggtcc
     3241 tggcgacatc cgcgcgtttt aaaacctcat cgaccagcat atgggtaaaa tcttcgccac
     3301 caagaaagtt gtcgccagcg gaggcgtgaa cttcaatcac cggcgtggcg tactcaagca
     3361 ccgtaacgtc aaacgtgccg ccaccgagat caaacaccag cgaacgggta ttttgttggg
     3421 tatgcaggcc atacgccatc gcagctgctg tgggttcatt aattaagcgt accgcattta
     3481 acccggctaa ctccgctgct aaacgggtat gcttgcgttg ttcatcgctg aaataagccg
     3541 gaacggagat caccacatct ttaatcggac gttgcagaaa ttcttcggca tcttctttta
     3601 atgagcgtaa taccaaagag gacagttctg gcgcgttaaa tgtgtcgctg cctaaccgcc
     3661 agttggtatt actgcccatt gcacgtttaa ataacgctgc cgttttatcc ggatgcgaag
     3721 tacgccgtga tacagccggt tttccgacta aaatatgatt attttcatcc atgctaatta
     3781 tggatggcgt taaatattca ccaaacttat ttggaattaa ttgcgcggca ccgtctttcc
     3841 agacggcaat taaactattg gtagtgccga gatcgatacc aatggcgagt tctgcattat
     3901 ccattgcatt tatatcttga aagaaaaaga gataaacaga ttaagaccac ccaggtaacg
     3961 cgtggtgatc ttaatcaatg acgtgtgtta agcgtaaaat ttcagacgat cagccagtaa
     4021 atcaacaaag ccctgacgat caacatcgac cattacagtg gcattcggtt tattgccagt
     4081 cagataataa taatcaacaa ccgtcatacc ctggctatat ttcccctgtg tttccacgcc
     4141 aacccagcgc tcaacagtgg taaacaactc gggtttcaac agccaggcga tggtgcatgg
     4201 gtcatgcagc ggtgcaccga caaagcccca tttttcgtct ttatgatatt cgaggaagaa
     4261 atccagcagt tcggcaacaa tggttgaaac agggttgcca atcgcgcgga aacgctcggt
     4321 gtcttcaacg tggatttgtg ctttgtgagt aacatccaga ccggccatca ccactgggat
     4381 ccctgactgg aagacaattt ctgccgcttc cgggtcaacg taaatattaa attcagccgc
     4441 aggcgtccag ttaccaagcc ccattgcgcc gcccataatc acgatacggg caattttgct
     4501 atgcagttcc ggatggctat tgagcagcaa ggcaacgtta gtttgcggtc cggtagacac
     4561 aatcgtgaca ggttccgcac tttcgcgcag cgttttcgcc atcagctcta ccgccgtaca
     4621 gttttgcggt gcgaacgtcg gttccggtaa tgccgggcca tccagaccgc tttcgccgtg
     4681 cacattgtcc gcgataatca actcacgcat taacggtttt accgcgccgc ctgctaccgg
     4741 aatatcggtg cgattaagca aggtcagcat acgcagaaca ttgcgtaagg ttttttctgg
     4801 tgtctggttt ccggcggaag acgtaattgc tttgacatca agctctggtg aggcgagggc
     4861 gagaactatt gcgatagcgt cgtcatgacc tgggtcgcaa tctaacagaa ttggcagtgc
     4921 cattgttgct ccttgttgtg tgcttctttg cgacaagggt aacgccagga tgtaacagat
     4981 acgaggggcg aaacgataaa gcgtgagatg gcgcgcactt gggtatgcgc gccagagtga
     5041 ttaatgcagg attttcgcga ggaagtcttt cgcgcggtcc gatttcggat catcgaagaa
     5101 agcgtctttc ggcgagtctt cgacaatttt accctcgtcc ataaagatca cccgattcgc
     5161 cactttacgg gcaaaaccca tttcgtgggt caccaccatc atggtcattc cttcgtttgc
     5221 cagttccacc atcacgtcca gcacttcgtt gatcatctcc ggatccagcg ccgatgtcgg
     5281 ttcgtcgaac agcatcgcaa taggatccat acacaacgcg cgagcgattg ccacacgctg
     5341 ctgctgaccg ccggaaagct gcgccggaaa tttattggca tgtgcggaaa gtcccacgcg
     5401 ctccagcaat ttcaatgctt tttcacgagc gggcgcttta tcgcgtttaa gcactttcac
     5461 ctgcgccagg gtcaggtttt cgataatcga cagatgaggg aacagctcga aatgctggaa
     5521 taccatcccg acgcgggaac gcagctttgc cagatcggtt ttcttgtcat taaccacgat
     5581 accatcgacg gtgatttcac cttgctgcac tggttcgagg ccgttgacgg ttttaatcag
     5641 cgttgatttg ccggaaccag acgggccgca aaccaccacc acttcgcctt ttttcacttc
     5701 ggttgagcag tcggtcagca cctgaaagtg accataccat tttgaaacat ttttcagggt
     5761 aatcattatg ctgtccttct tttcaagtag ctgaccaaca acgacgcgct aagactaata
     5821 acgaaataaa caaatccggc aaacaggatc atctcaacct gcgtaccatc acgctcacca
     5881 atggttgagg cggtacggaa gaaatcggcc agagataaca catacaccag tgaggtatcc
     5941 tggaacagta cgatgccctg agtgagcaga agcggcacca tcgcgcggaa cgcctgcggc
     6001 agaataatca gtttcatcga ctgccagtga gtcattccca acgccagcgc ggcgctcgac
     6061 tgaccacgag aaatactttg aataccagca cggataatct ctgaatagta ggccgcttca
     6121 aacatcgaaa acgccaccat cgccgaaatt aaacggatat cattttttgg tgataatccc
     6181 agcacgtttt gcagaaaacc tggcacgatc aggtaaaacc acagcaaaac cataactaaa
     6241 ggaatcgagc ggaatacgtt aacgtaggct ttggcaaacc acgctacggg cgcaaagctg
     6301 gacaagcgca tcaccgccag catggtgccc cacaaaatac caatcacgac tgccgtgacg
     6361 gtgattttca gcgtgatcac cagcccgtcg agcagatatg gcagggaagg gacaatggaa
     6421 ctccagtcaa actcgtacat tatttgcccc ccatgttgcc aggcaggcga actttacgtt
     6481 caaccagcgt cattaccagc atgataaccg cgttaatcaa cacatacgcc agcgtaatag
     6541 cggtaaacga ctcccaggcg tgggctgagt aatcaagcaa tttacccgcc tgcgccgcca
     6601 tatccaccag accaatagtc gaggcgatgg cggagttttt caccaggttc atcatctcgg
     6661 aagtcatcgg cgggacgata acgcgataag cattaggcag cagtacgtat cgataagcct
     6721 gcggtagcgt caggcccatc gccagtgcgg catttttttg cccacgcggc aacgactgaa
     6781 tggcggcgcg tacctgttcg caaacacggg cggcggtaaa cagccccagg cagagcatgg
     6841 atgaaaggaa aaactgaata ttgggatcca gctcggcctt aaaccacatg ccgattttct
     6901 ccggcaacag ctccgggatc accagatacc aggtaaagaa ttgcacaatc aacggcacgt
     6961 tgcggaacag ttcgacatac aacgtaccca gaccggagag aaaacggttt ggaacggtac
     7021 gtaaaatgcc gaaaaatgaa ccgacgagga aagcgataat ccaggcgcag atcgacaaag
     7081 cgatcgtcac ctgaaaacca ctccagatcc aaccaagata ggtggtgttg ccgaacgggg
     7141 cttgttgtaa aaaaataccc cagttccagt ctatagacat aaatctactc cagaaaaaag
     7201 agggtagcag cgttaactgc taccctcgaa gattgttacc cagcgtattg cggttttcag
     7261 gcccgatggg gaacgaccat caggcgtata gtctgtccgt gctacgtaac aatcgagagg
     7321 gctggaattt ccgcccctgg ttcttgtaat tagttcagtg ctttgtcatt cggttctttg
     7381 aacagtgctt tcatttcgtc tgacagttcg aaattcatgt tcaggttttt cggcggaatt
     7441 ggatttttga accatttatc aaaccatttt tccgcttcac cggaagtctg cacctgagcg
     7501 atagtgtcat ccatcagctt tttgaactgc ggatcatctt tacgcaacat gcaaccgtag
     7561 gcttcctgag actgcggctt gctgacgata tcccagttgt ctggtttctt cgctttcgca
     7621 cgttcaccgg ccagcagagc gtcatccatc ataaaggcaa cggcacgacc gctttccagg
     7681 gtacggaaag agtcaccgtg atctttggcg ctaatgatgc gcatattcat tttttgctct
     7741 tcattcagtt tgttgagcaa aacttcagaa gtagtgccgg aagtgacgac tacggctttg
     7801 cctttcaggt cggcaaaatc tttgatatcg ccgccctttt tggtcagcag gcgcgtaccg
     7861 accacgaaaa tagtatcaga gaaagccgcc tgtttttggc gttcgacgtt gttggtggta
     7921 gaaccacatt caaaatcgaa agtgccgttt tgcagcagtg gaatacggtt ttgtgaggta
     7981 atcggaatca gttttacctg caagtccggt ttgttgagtt tctttttcac tgcttcaaca
     8041 atggcgttgg agtaatcctg cgagtaaccc accacttttt gctgattgtc gtaataagag
     8101 aaaggtactg aagattcacg gtgaccgacg acaatcacgc cgtttttggc gattttgtcc
     8161 agcgtactgc ccgctaccgg ggcggcgtca tctgcctgtg ccagtcctgc ggaaagcgcc
     8221 agggcgagga ttgctgtggc aggtttacgt aattgcatat ccaactcctt tatcttctgc
     8281 gttaagaacg catggatacc cgttgtgagt gtttgtgttg ttatcgtctg caactttatt
     8341 gtgcagtgtt gtgcctgtta ggtaacattt agtttggcta aatgtaaaga tattgctgtt
     8401 ttattgtttg tttttgcgag atgcgccgca ccattccgaa gcaaaattct taaaccgcac
     8461 ctttttggtg ctaccgctga tttgtggtgg tgcaacctgg ttgcgtcgcg gctgatttca
     8521 tcgcttacac agtaaataaa gcaacttaca tgccaaaaaa tacttagggg cgaattaatt
     8581 acgattgtaa ttttaggctg aaaaagtcat atttcggaca gaattatctt ttcggcatgg
     8641 gacgtttgcc gggatgataa ctgcacacgg gaaaaatgct ttttacctgt cttttcaagg
     8701 aagaaacacc ttatgtctgc atcttcggtt aagccgttaa atgttcaatt acccgcaata
     8761 acccttatcc tttttgcact ctgtattggg atattttgtt atctcgcaca atggatgagt
     8821 tatgaagagg tcgatcaatc cgcacttatc catctcggcg caaacgttgc ggctcttact
     8881 ttgtcgggtg aaccctggcg cttattgagc agtatctttc tgcacagtag tgtttctcat
     8941 ctgctgatga atatgtttgc attcctggtc gtgggaggcg tggcggaaca gattctgggg
     9001 aaatggcgac tcctgattac ctggttattc tccggcgtct ttggtgggct catcagcgcc
     9061 tgttatgcgt tacgcgatag tgatcagata gtcatcagcg ttggggcatc cggggcaatt
     9121 atgggaatag ctggcgctgc gatagcaaca cagcttgctt caggtgcggg cacataccat
     9181 aaaaaccagc ggcgagtatt ttctctgttg ggtatggtgg cgctgacact gttgtatggt
     9241 gcccggcaaa caggaataga taatgcttgt cacattggcg gcctgattgc gggtggcgcg
     9301 ttgggttggc tgagcgcgcg tttatctggg caaaaccgac tcgttacgga aggcgggatt
     9361 attgttgcgg gcagtcttct tctgaccggg gctatctggc ttgcgcagca gcagatggat
     9421 gagtcagttt tacaagtcag gcaaagcctg cgtgaagcgt tttatccaca ggagattgaa
     9481 caagagcgac ggcaaaaaaa gcagcagtta gcggaggaac gcaacgccct cagggaaaca
     9541 ttatccgctc cggtaagtcg tgaacaggcc agtggtgatt tgctcgctga gattgccgat
     9601 atccatgata tggcgatcag tcgggatggt aatatgttgt atgccgcaat tgaaaacacg
     9661 aacagcattg ttgttttcga cctcggacaa aagaaaatcc tgcatacctt tacagcgccc
     9721 atagcgaaag aaaagtcagt caaacattgt ggtggctgta aagatcaggg cgtcagatcg
     9781 ctggcattaa gcccggatga aaagttgatt tatgcgactt catttgaagc gaatgcgtta
     9841 tcggtcatta acgtggcgac aggggagatt attcagtcga ttaccaccgg tgcacatcct
     9901 gacagcctta tcctctcgcg tgatggcaca aaagcctggg tgatgaatcg caccagtaat
     9961 agtgtgtcag cgattgatct ggtgacttat cagcatgtgg cggatatccc gctggagaaa
    10021 tacgacggga cggggacgag cggtaaacca ggcgcctggg ttatggcact ttccccggat
    10081 gaaaaaatat tgttgatacc cggtatggtc agaggtgaca ttgtacgcat caataccatc
    10141 acgcatcaga aagaaagcta tccggctagt gatgcgcgag gaacgatatc ggcgatgcgt
    10201 tttcgacctg aaaacgggga tgtaattttt gccgacagcc aggggatttc acgtataagc
    10261 gtaggggatc aacaagccag cattatgacg caatggtgta gcaggagcgt ttattccgtt
    10321 gagggtatta gcccggacgg tcagtattta gcgttggtgt catatggcct gcaaggttat
    10381 gtcatcctgc tcaatattaa tgccgggcag attattggcg tttatcctgc cagctacgtt
    10441 aatcaccttc gtttttcagc ggatggtaga aaaatatttg ttatggcgaa gaacgggttg
    10501 atccaactgg acaggacgcg ctcgcttgat ccgcaggcaa ttattcgtca tccccaatat
    10561 ggcaatgtgg cttgtatccc tgaaccgtaa gaaaaagggg catgataacc atgccccaaa
    10621 gctcaatgag aaagtacgtt ttgctcatgc aggatacatc aggcacatca acgtttacgt
    10681 gctcgcagac tcatcagcac agcggcaaaa ccaaacagtg ccgtcagcac ccacagcggc
    10741 cagttgccgg tacgtgcgta tggtgtgagt ccggtggtcg gcgtcacgtt agtggttaac
    10801 acctcgcggg tgaactgcgg gatcatcgcc tgaatctcac cctgcgggcc aatcaccgcc
    10861 gtaatgccgt tgttggtgct gcgcaacagt gggcgcgcca gctccagcgc acgcattcgc
    10921 gccatctgga agtgttgcca tggaccaata gatttaccaa accacgcatc gttggagata
    10981 gtcagcagat agtcggtatc cgggcggaag ttatcgcgca cttgctcgcc aagaatgatc
    11041 tcgtagcaaa tggccgcagt aagctcaata ccatttaccg acagcggcgg ctggatatat
    11101 ggcccacggc tgaacgacga catcggcaga tcaaagaacg gtgctaacgg acgcagaatc
    11161 gactccagcg ggacaaactc gccaaacggc accagatggt ttttgttata gcgatcggct
    11221 gattcgtagc tgtacggtgc acctttaccc agtgtgatga tggtgttgta ggtatcgtag
    11281 cggttctgct tattgagacg cgcgtcgaca atcccggtta ccagcgagct acctttatcg
    11341 cgcaattcac cgtccagtgc tttgaggaac ggttgctgat taatttccag atcggttatc
    11401 gccgactccg gccagataat caacgatgat ttgcccatca gcggagccgt tgcgttgtag
    11461 taaatcttca gcgtattaag aagctgatct ccatcccatt tcagtgattg cggaatatcg
    11521 ccctgaacca tcgaaacctg aatggttttc tccggttgcg gggtaaacca ttggatgtaa
    11581 cgcagcggga agggtagggc aaacagcacg acggccacca ccagcggacg ccagttgcgt
    11641 ttgaccaacg ccagtgccag caggccacta accatcatca gcaggaagtt aatggcttcc
    11701 acgcccatta tcggtgccag cccttttaac gggccatcaa tctggctata gccgaactgt
    11761 aaccacggga agcctgtcag tacccaaccg cgcagaaact cggtcacttg ccagagagaa
    11821 ggggcggcaa tcgccacgcg cagccaggtg gttttcggcc acagacgcga cagcacgcca
    11881 gcaaacagtc cggtatacag cgacaaatac gccgccagca gcaccaccag gaagatgtta
    11941 accgggccag gcatcccgcc aaaggtcgcg atgctgacat agacccagtt aataccgctg
    12001 ccaaagaggc caaatcccca gcaaaagcca atagcggcag actggagtgg gcgacggtta
    12061 aaggtcaacg cctgaagccc catcagcgaa ataatcgccg caggccagac gtcgtaagga
    12121 gagaaggcca gcgttccgca ggcaccgaat aataacgcca gcagcaggcg aatgcgctgg
    12181 cgttcaatta atgaggcaaa agccatgtag ttatctatcc agtttcggtt ttattcatcc
    12241 agctttggct ggggtgagtc atccgggatt ttgacatgaa cctgaataat acgccgactg
    12301 tcggccatcg ccactttgaa ctggtaaccg tcgatgtcga tggtttcacc acgcgctgga
    12361 agatgcccaa acgcctgcat caccagacca ccgatagtgt cgacctcttc atcgctaaag
    12421 tgggtgccga acgcttcgtt gaagtcttca atggaagcca gtgcgcgcac ggtccaggta
    12481 tgacgactca gctgacggaa gtcgatatca tcttcttcgt catattcgtc ttcaatctca
    12541 ccaacaatca gttccaggat gtcttcaatg gtcaccagac cggaaacccc accgaattcg
    12601 tcaatgacga tcgccatgtg gtaacgctga gagcgaaact ctttcagcat ccggtctacg
    12661 cgcttacttt caggaacgac aaccgcctga cgtaacactt tgtccatgct gaaggcttca
    12721 gcatcgctgc gcataaacgg cagcaagtct ttcgccatca gaatcccttc aatgtgatct
    12781 ttgtcttcgc taatcaccgg gaaacgtgag tgggcggact cgatgatgac atcaaggcat
    12841 tcgtccagcg tctggttgcg tttcagggta atcatctggg agcgggggat catgatgtcg
    12901 cgaacgcgtt ggtctgcgat gtccatcacc ccttcgagca tatcgcgcgt atcttcgtcg
    12961 ataaggtcgt tctgcccgga atcacggatc agcgccagca gttcgtcacg gtttttcggt
    13021 tcaccgtgga aaagttggct gagtaacagg gagaaaaatc ccttcttgtt gcttatcgtg
    13081 tcactactgt gtgaattgtc gtcgctcatg gcgtcgtatg ggttctcatg ttagttaatc
    13141 aaaacgccgt cgttaatcac caacggcggg gacgtctgcc agtcaaatgc ctggcaaatt
    13201 attctttctc ggcaatgtac ggatcctcat agcccagagc aagcataatc tctgtttcga
    13261 gggcttccat ttcttctgct tcgtcatctt cgatgtgatc gtaacctaat aaatgcagac
    13321 tgccgtgcac caccatatgc gcccagtgcg cctccagtgg tttgccttgc tcctgagctt
    13381 ccttctcaac cacctgacgg cagataacca gatcgcccag tagcgacatt tccatgccag
    13441 gcggcacttc aaacgagaag gagagcacgt tggtcggctt atccttaccg cgataggtca
    13501 gattcagact gtggctttcg gcggtatcga ccacgcgaat agtcacttcc gattcttcct
    13561 gaaactgcgg gatcaccgca ttcagccatg tttgaaactg gctctcttcc ggtaacccgg
    13621 aattatcttc acatgccagt tgtaaatcga ggatcacctg actcattttt gttcctgttc
    13681 ttcgcgcttg cgttctgccg ccagcgccgc ttttcgtttt tgttcggctt cttcccaggc
    13741 ttcataggcg ttaacgatac gcgccaccac agggtgacga accacgtctt cgctgtggaa
    13801 gaagttaaag ctgatctctt cgacatcggc cagcacttcg atggcgtgac gtaagcctga
    13861 tttagtatta cgcggcaggt cgatctgtgt gacgtcgccg gtgataaccg cttttgagtt
    13921 aaaaccgata cgggtcagga acatcttcat ctgttcgatg gtggtgttct ggctctcatc
    13981 gagaatgata aacgcgtcgt tcagcgtacg accacgcata taggccagcg gcgcgacttc
    14041 aataacgttg cgctcaatca gtttctcaac tttctcaaag cccagcattt caaacagcgc
    14101 gtcgtacagt gggcgcagat acgggtctac tttctggctt aaatcgccag gcaggaagcc
    14161 cagtttctca ccggcttcga ccgccggacg agtcagcaga atacggcgaa tctcctgacg
    14221 ctccagggca tcaactgccg cagccactgc caggtaggtt ttacccgtac ccgccgggcc
    14281 aacgccgaag gtaatgtcat ggtcgagaat attggcgatg tactgcgcct ggtttggcgt
    14341 gcgcggctta attacgccgc gtttggtttt gatattgacc gctttgccgt actccggcac
    14401 gctctccgca ctttgctcca gtactcgcgc ttctttaatc gcaaggtgga tctgttccgg
    14461 ttcgatatcc tgaatctgac cgcgcatcgg ggctgtatcg acatacaggc tacgcagaat
    14521 gtctgccgca gcggtgacgc aaatcggacg gccggtcagt ttaaagtggt tgtcgcggcg
    14581 attgatctcg atgccgaggc ggcgttcgag ctgcttgatg ttgtcatcaa acgggccgca
    14641 caggctcaac agacgcgcgt tgtctgctgg ctccagggtg atttcgcgag tgtctatgtt
    14701 caaaccgttc ctcttatctg tatgccgccg gaagctgaac attcaccggc ctataaggaa
    14761 attattcacg ccacaggaaa aaggcgcaag cattgcgata aagatgggga taaagagaga
    14821 aaaaacaagg cccaccggaa cggcgggcct gagaattacg gctgataata acccacgcca
    14881 aggtcgtttt ctttgcgggt acgggcaatc accgattccg gtgtttctgc cacgcgcaga
    14941 cccatttcat cttcagtacg caccacttta ccgcgcagag agttcgggta gacgtcggta
    15001 atttctacat cgacgaattt accgatcata tccggtgtgc cctcgaagtt gaccacgcgg
    15061 ttattttcgg tacgcccgga aagctccatg atgctcttac gcgaagtccc ttctaccaga
    15121 atacgctggg tggtgccgag catccggcgg ctccatgcca tcgcttgctg attgatacgt
    15181 tcttgcagaa tatacagacg ttgcttcttc tcttcttccg gaacatcatc aaccatatcg
    15241 gcggctggtg tacccggacg tgcagagaag ataaagctgt agctcatgtc gaaattgacg
    15301 tcggcaatca gcttcatcgt tttctcgaag tcttcggtgg tttcgccagg gaagccaacg
    15361 atgaaatcag aactgatctg aatatctgga cgcgccgcac gcagtttacg gatgatcgct
    15421 ttgtactcca gcgccgtatg ggtacgtccc atcaggttca gaatgcgatc ggaaccgctc
    15481 tgtaccggca gatgcaggaa gctcaccagc tccggcgtgt cgcgatacac ttcgatgata
    15541 tcgtcggtga attcgatcgg atggctggtg gtaaagcgaa tacgatcgat cccgtcgatc
    15601 gcagcaacca gacgcaacag atcggcaaac gatccggtgg tgccgtcgta gttctcacca
    15661 cgccaggcat tcacattctg accgagcagg ttgacttcac gcacgccctg agccgcaagc
    15721 tgggcgatct caaacagaat atcgtcggac ggacggctta cctcttcacc acgggtgtaa
    15781 ggcaccacgc agtaggtgca atatttattg cagccttcca tgatggagac aaacgcggtc
    15841 ggcccttcgg cgcgcggttc cggcagacgg tcaaacttct cgatttccgg gaagctgata
    15901 tctacaaccg ggctgcggtc gccgcgcacg gagttgatca tctccggcag acggtgcagc
    15961 gtttgcggcc caaaaataat atcgacatag tgggcgcgct ggcgaatgtg ctcgccttct
    16021 tgcgatgcca cgcagccacc gacgccgata atcaggtctg gattcttctc ttttaacagt
    16081 ttccagcgac ccaactgatg gaagactttt tcctgagcct tctcgcggat tgagcaggtg
    16141 ttcagcagca gcacatccgc ttcttccgcc acgtcggtca gttgatagcc gtgggtggca
    16201 tccagcagat cggccatctt cgatgaatcg tactcgttca tctgacagcc ccaggtttta
    16261 atatggagtt ttttggtcat cgacttgctc ttgcgaaata gtagccagga atgcagggcg
    16321 catagtgtaa tgctttgctg ccgttgtgac cagtatgagc gttatcagcc cttgggggta
    16381 aaaatcctgt aaacttaaag catattgcta acaggatgat tgaccatgac aaatcaacca
    16441 acggaaattg ccattgtcgg cggaggaatg gtcggtggcg cgctggcgct ggggctggca
    16501 cagcacggat tttcggtaac ggtgatcgag cacgcagaac ctgcaccgtt tgtcgctgat
    16561 agccaaccgg acgtgcggat ctcggcgatc agcgccgcat cggtatcatt gcttaaaggg
    16621 ttaggggtct gggatgcagt acaggctatg cgttgccatc cttaccgcag actggaaacg
    16681 tgggagtggg aaacggcgca tgtggtgttt gacgccgctg aacttaagct accgttgctt
    16741 ggctatatgg tggaaaacac cgtcctgcaa caggcgctgt ggcaggcgct ggaagcgcat
    16801 ccgaaagtaa cgttacgtgt gccaggctcg ctgattgcat tacatcgaga taatgatctt
    16861 caggagctgg aactgaaggg cggtgaaacg atccgcgcga aactggtgat tggtgccgac
    16921 ggcgcaaatt cgcaggtgcg gcagatggcg ggaattggcg ttcatgcctg gcagtatgcg
    16981 cagtcgtgca tgttgattag cgtacagtgt gagaacgatc ccggcaacag cacctggcag
    17041 caatttaccc cggacggacc gcgtgcgttt ctgccgttgt ttgataactg ggcatcgctg
    17101 gtgtggtatg actctccggc gcgtattcgc cagttgcaga atatgaatat ggcgcaactc
    17161 caggcggaaa tcgcgaagca tttcccgtcg cgtctgggtt acgttacacc gcttgccgct
    17221 ggtgcgtttc cgctgacgcg ccgacatgcg ttgcagtatg tgcagccggg gcttgcgctg
    17281 gtgggcgatg ccgcgcatac catccatccg ctggcggggc agggggtgaa tcttggttat
    17341 cgtgatgtcg atgccctgat tgatgttctg gtgaatgccc gcaactacgg tgaagcgtgg
    17401 gccagttatc ctgtcctcaa gcgttaccag atgcggcgca tggcggataa cttcattatg
    17461 caaagtggta tggatctgtt ttatgccgga ttcagtaata atctgccgcc gctgcgtttt
    17521 gtgcgtaatc tcgggttaat ggcggcagag cgtgctggcg tgttgaaacg tcaggcgctg
    17581 aaatatgcgt tagggttgta gccttacaac attgccggat gcgtgccaac cgtaggtcgg
    17641 ataagacgcg gcagcgtcgc atccgacatt gaaggataag acgtgtcaac atcgcattcg
    17701 acattgaatg aacgcagaaa agcaaaaagc tcgccgaagc gagctttttt aatgtggctg
    17761 gggtacgagg attcgaacct cggaatgccg gaatcagaat ccggtgcctt accgcttggc
    17821 gataccccaa caaattggtt tttgaatttg ccgaacgtat tcgacacatt cagaatttgg
    17881 tggctacgac gggattcgaa cctgtgaccc catcattatg agtgatgtgc tctaaccaac
    17941 tgagctacgt agccagattg tttcttcgat ggctggggta cctggattcg aaccagggaa
    18001 tgccggtatc aaaaaccggt gccttaccgc ttggcgatac cccatccgta caacgctttc
    18061 tggtgaatgg tgcgggaggc gagacttgaa ctcgcacacc ttgcggcgcc agaacctaaa
    18121 tctggtgcgt ctaccaattt cgccactccc gcaaaaaaga tggtggctac gacgggattc
    18181 gaacctgtga ccccatcatt atgagtgatg tgctctaacc aactgagcta cgtagccatc
    18241 tttttttcgc gataccttat cggcgttgcg gggcgcatta tgcgtataga gccttgcagc
    18301 gtcaacctct ttttcaagga aaattgctcg aaagtgactg tttggttagg ttgcgaacag
    18361 cgtggcgcta tattcgtcaa ttattgttta ctttgtgttt tttccccacc ctacagccat
    18421 tcttttgtca tacaggatga aattcggaat tcaacaatgg tggtgggaaa ttaatctatg
    18481 aaatactggc ctacagtgat gagttgtcaa acagtgatgt ggcaaacccg gaacatttcc
    18541 ttactgcata tcagaatcaa caagcacctc aataactgaa acagccccgg atttcaccgg
    18601 ggctgtttcg catttcttac ttatacgccg actggtgaac gccaaccgca cgaccagacg
    18661 gatcgtccat tttcttgaac gcctcatccc actcaatcgc tttagcggaa gaacacgcga
    18721 cggacgggcc gcccggcacg cactcagcgg cgctcggaag cgggaacagt tcttcaaaga
    18781 tctcccggta cagatacgct tcttttgagg ttggcgtgtt gtacgggaag cggaagcggg
    18841 cagtttccag ttgctgatca gaaacctgct gcgccgccac ttctttcagg gtgtcgatcc
    18901 aactgtaacc gacgccatcg gagaactgct ctttctgccg ccaggccacg cttgcgggca
    18961 ggtatgactc aaaacattca cgcaggatgt gtttttccat tttgccgtta ccgcacattt
    19021 tatcctgcgg gttaatgcgc atcgccacat caaggaattt tttgtcgagg aacggaacgc
    19081 gtgcttccac gccccaggct gacatcgctt tgttggcgcg cgcgcagtca tacatatgca
    19141 gggccagcag tttacgcacc gtctcttcat gcagttcttt agcgttgggc gctttatgga
    19201 agtaaagata accgccaaac acttcatcag aaccttcacc ggacagcacc attttaatgc
    19261 ccatcgcctt gatcttacgc gacattaaat acatcggtgt tgaagcgcga atagtggtca
    19321 catcataagt ttcgatgtgg taaatcacgt cgcggatggc atccagacct tcctgtacag
    19381 tgaagtgaat ttcgtgatgc accgtgccca gatggtttgc cacttcctgg gctgctttaa
    19441 gatccggtga acccggcaga cctacagcaa aggagtgtaa ctgcggccac caggcttcag
    19501 agcgttcctg atcttccacg cgacgggctg cgtatttctt ggtgatagcg gaaataattg
    19561 aggaatccag accaccagaa agcagcacac cgtaaggcac atcagacatc agatggcttt
    19621 taacggaatc ttccagagcc tgacgcagct cgtttttgtc ggtcacgtta tctttcactg
    19681 catcaaagtc gaaccagtcg cgatgataat aagaacggat ttcgccgtcc tggctccaca
    19741 aatagctccc cgccgggaac tctttaatcg tgcggcaaac tggcaccagc gatttcattt
    19801 ctgaggccac atacagctga ccgtgttcgt catagcccat atacagtggg atgatcccca
    19861 gatggtcgcg accaatcagg taggcatctt tttcgctgtc gtacagggca aaggcaaaca
    19921 tgccctgcaa gtcgtcgaga aattccggcc ctttttcctg atacagcgcg aggatcactt
    19981 cacagtcaga ccctgtctgg aactgataac gatcgccata ttcagcgcgc aatgcctggt
    20041 ggttgtagat ttcaccgttt accgccagta cgtgggtttt ttgttggttg tagagaggtt
    20101 gcgcccccgc gttaacgtca acaattgaca gacgttcgtg agcgagaatg gcgttatcgc
    20161 tggcataaat accggaccag tccgggccac gatgacgcat caggcgtgac agctcgagtg
    20221 ctttcttacg cagctcaact gcgtctgttt tgatatcgaa tacgccaaaa attgaacaca
    20281 taaccttctc cgttaacctg gtatttgttg cttgttgtgt ttgcttgttt aaaaaaatgc
    20341 cgcaaagcag cactgtgcgc aagcgatttg gcggtgaaaa aataaaaaac gtaatggtga
    20401 ttgtcgattg gtgaaaaatg gtctggtgtg aggatgtatt tattgatgaa tcgataattt
    20461 ttagcgggtt ttattgaatg gttatatttt acgggggcca aattgctgat aaagtgcgat
    20521 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaagcat gaaaattcaa
    20581 tatattgcag gagctgcgta ggcctgataa gcgtagcgca tcaggcagtt tggcgtttgt
    20641 catcagagcc acccacgtcc gcagacgtgg gtggtattca gataacgtcg atttcagcga
    20701 ctgacgggta aatccagctt gggcggaacg gcatactgtc gatatcgtcg agcgatgaaa
    20761 caccagaaag caccagaatc gtttccagac ctgcctggaa gcccgccaga atgtcggtgc
    20821 gcaagttatc gccgacaatc accgtttctt ccgaatgcgc ctgcattttg tttaatgctg
    20881 cgcggatgat ccacgggctg ggcttaccaa catagaacgg tttgcgcccg gagattttct
    20941 caatccctgc acacaacgcg ccacaagcgg gataaaaacc gcgcccgtgg gtgtccgggt
    21001 tggtggcgat aaagcgtgca ccgttagcga cgaaataggc cgctttatgc atcatgtccc
    21061 agttgtagga acgcgtttcg ccaacaatca cgaaatcagg gttcacatcg gtaatagtga
    21121 aaccggcttt gtacagttca tgaatcagtg cgccttcgcc caccaca
//
LOCUS       DAJSKJ010000057        21077 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000057 DAJSKJ010000000
VERSION     DAJSKJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.057"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ON345_001836"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ON345_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR3071903.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ON345_001837"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ON345_001837"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR3071904.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ON345_001838"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ON345_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR3071905.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ON345_001839"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ON345_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR3071906.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAYRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ON345_001840"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ON345_001840"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR3071907.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ON345_001841"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ON345_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR3071908.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11273
                     /gene="ftsK"
                     /locus_tag="ON345_001842"
     CDS             7245..11273
                     /gene="ftsK"
                     /locus_tag="ON345_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3071909.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRQQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATRAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVSVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            11428..12039
                     /gene="lolA"
                     /locus_tag="ON345_001843"
     CDS             11428..12039
                     /gene="lolA"
                     /locus_tag="ON345_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR3071910.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12050..13393
                     /gene="rarA"
                     /locus_tag="ON345_001844"
                     /gene_synonym="mgsA"
     CDS             12050..13393
                     /gene="rarA"
                     /locus_tag="ON345_001844"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR3071911.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13484..14776
                     /gene="serS"
                     /locus_tag="ON345_001845"
     CDS             13484..14776
                     /gene="serS"
                     /locus_tag="ON345_001845"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR3071912.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15015..17459
                     /gene="dmsA"
                     /locus_tag="ON345_001846"
     CDS             15015..17459
                     /gene="dmsA"
                     /locus_tag="ON345_001846"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR3071913.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITEKLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17470..18087
                     /gene="dmsB"
                     /locus_tag="ON345_001847"
     CDS             17470..18087
                     /gene="dmsB"
                     /locus_tag="ON345_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR3071914.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18089..18952
                     /gene="dmsC"
                     /locus_tag="ON345_001848"
     CDS             18089..18952
                     /gene="dmsC"
                     /locus_tag="ON345_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR3071915.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(18987..19613)
                     /locus_tag="ON345_001849"
     CDS             complement(18987..19613)
                     /locus_tag="ON345_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3071916.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAEFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19927..21075
                     /locus_tag="ON345_001850"
     CDS             19927..21075
                     /locus_tag="ON345_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3071917.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         5199 a         5290 c         5605 g         4983 t
ORIGIN      
        1 ttgtcgcctg acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tggccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcacctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agctctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcagcaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac gataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgataggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gcaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca caccggtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccacgcaga cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gctgccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgctgt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca acgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgcc tgttgcagac gatcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagcctgtac cggccgaagt aatggcctga cgataaatat gatccatcac gtcacccgcg
     5221 gcaaagacgc cagggatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaga gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgtcgcctt ctggaagcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggctggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcaacagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagtact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgctgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg aatatgaaga tgacgaagag tatgaagatg
     7921 aaaaccacgg caaacagcat gaatcacgcc gtgctcgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgctgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattatgcaa ctgcgccgga acagcctgcg caacagccgt
     8581 attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc
     8641 aatccacttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
     8701 ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg
     8761 aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg
     8821 aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac
     8881 cggttaaaga accagaaccg atcaaatctt cgttgaaagc gccttctgtt gcagcagtac
     8941 ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
     9001 tggcgacgag ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg
     9061 gaccgcgtcc gcaagtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
     9121 gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa gctgccctca cagcgtgcgg
     9181 cggaagagaa agcccgtgaa gcccagcgca atcagtatga ttctggcgat cagtataacg
     9241 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
     9301 agcaacgcta tggcgaacag tatcaacatg atgtgtccgt aaacgcagaa gatgcagatg
     9361 ctgcggcaga ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg
     9421 gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga
     9481 aagcgttgct ggatgatggt ccacacgaac cgttgtttac gccaattgtt gaacctgtac
     9541 agcagccgca acaaccggtt gcgccacagc agcaatatca gcagccgcaa cagccggttg
     9601 cgccgcagcc gcagtatcag cagccacaac agcaggttgc gccgcagcca caatatcagc
     9661 agccgcaaca accggttgcg ccacagccgc aatatcagca gccgcaacaa ccggttgcac
     9721 cgcagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac agccgtccgt
     9781 tgcataaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg ccgagcgaag
     9841 tggagccggt agataccttt gcgcttgaac aaatggctcg cctggtggaa gcgcgtctgg
     9901 ctgatttccg tattaaagcc gatgtcgtca attactctcc ggggccggtt atcactcgct
     9961 ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg tcacgggacc
    10021 ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct ggcaaaccct
    10081 atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc gaagttttgg
    10141 ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt aaagatatcg
    10201 ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt gcggggacta
    10261 ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt tataaagcac
    10321 agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt tcggtttatg
    10381 aaggcattcc gcatctgtta acggaagtcg ttactgatat gaaagatgcc gccaacgcgc
    10441 tgcgctggtg tgttaacgag atggagcgtc gttataaact gatgtctgcg ctgggtgtgc
    10501 gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg cgtccgattc
    10561 cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg ctgaaaaaag
    10621 aaccatacat tgtggtgttg gttgacgaat ttgccgacct gatgatgacg gtaggtaaaa
    10681 aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt atccacctcg
    10741 tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa gcgaatattc
    10801 cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt cttgatcagg
    10861 ctggcgcgga atcactgctg gggatggggg atatgctcta ctctgggccg aactccacgt
    10921 tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc gtggtgcagg
    10981 actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacttccgac agtgaaagcg
    11041 aaggtggcgc gggcggtttc gatggcgctg aagaactgga tccgttgttc gatcaggcgg
    11101 tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt cagttccgca
    11161 ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg attgtcagcg
    11221 aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac taattaatgc
    11281 attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa aattcagtat
    11341 tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga actcccgatc
    11401 gggagtgacg taatttgagg aataatgatg aaaaaaattg ccatcacctg tgcattactc
    11461 tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa aagccgcttg
    11521 gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg tagcggcgcg
    11581 gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt caactggcat
    11641 atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg gttctataac
    11701 ccgttcgttg agcaagctac ggcaacttgg ctgaaagatg ccaccggtaa tacgccgttt
    11761 atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa acagaatggc
    11821 gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt caccattaac
    11881 gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga tcagcgcagc
    11941 agttatcaac tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt taccttcacc
    12001 ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag tgagcaatct
    12061 gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc ggccagaaaa
    12121 tttagcacag tatatcggcc agcaacattt gctggctgcg gggaagccgt tgccgcgcgc
    12181 tatcgaagcc gggcatttac attctatgat cctctggggg ccaccgggta ccggcaaaac
    12241 aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta tttctgccgt
    12301 cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa accgcaatgc
    12361 aggtcgccgc actattcttt ttgttgacga agtgcaccga ttcaacaaaa gccagcagga
    12421 tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa ccactgaaaa
    12481 cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtctatc tgttgaaatc
    12541 cctgggtaca gaggatattg agcaagtact aactcaggcg atggaagaca agacccgtgg
    12601 ttatggtggt caggatattg ttctgccaga tgaaacacga cgcgccattg ctgaactggt
    12661 gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata tggcagaagt
    12721 caatgatagc ggtaagcggg tcctgaagcc tgaattattg accgaaatcg ccggtgagcg
    12781 aagcgcccgc tttgataaca aaggcgatcg cttttacgat ctcatttccg cactgcataa
    12841 gtcggtacgt ggtagcgcac ccgatgcggc gctctactgg tatgcgcgaa ttattaccgc
    12901 tggtggcgat ccgttatatg tcgcgcgtcg ttgcctggca attgcttccg aagatgtcgg
    12961 caatgccgac ccacgggcga tgcaggtggc aattgcggcc tgggattgct ttactcgcgt
    13021 tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg cctgcgcgcc
    13081 aaaaagcaac gctgtctaca ctgcgtttaa agctgcgctg gcggatgctc gcgaacgtcc
    13141 ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga aggaaatggg
    13201 ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg ccggtgaggt
    13261 ttacttcccg cgggaaatag cacaaacacg ctattatttc ccgacaaaca ggggccttga
    13321 aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata gccccataaa
    13381 acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt ggtcgcaggc
    13441 tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg atcccaatct
    13501 gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct ttaagctgga
    13561 tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca aaacggaaaa
    13621 cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag cgcgcgggga
    13681 agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg atgcagcaaa
    13741 agccgagctg gatgctttac aggctgaaat tcgcgatatc gcgctgacca tccctaacct
    13801 gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag tcagccgctg
    13861 gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg gtgaaatgca
    13921 ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg tggtaatgaa
    13981 agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc tgcataccga
    14041 acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca cgctgtacgg
    14101 tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc tggaagaaga
    14161 agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga ctaacctggt
    14221 gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc acaccccatg
    14281 cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc gtatgcacca
    14341 gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa gactcaatgg cggcgctgga
    14401 agagatgact ggtcatgcag aaaaagtcct gcagctgctg ggcctgccgt accgtaaaat
    14461 catcctttgt accggcgaca tgggctttgg cgcttgcaaa acctatgacc tggaagtatg
    14521 gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt gggatttcca
    14581 ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc gtctggttca
    14641 taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa tggaaaacta
    14701 tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata tgaacggact
    14761 ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgcctgcg ggcgcttttt
    14821 ttgtctccct ttgataccga acaataatta ctcctcactt acacgtaata ctactttcga
    14881 gtgaaaatct acctatctct ttgattttca aattattcga tgtatacaag cctatatagc
    14941 gaactgctat agaaataatt acacaatacg gtttgttact ggaatcaatc gtgagcaagc
    15001 ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt attggctgct gaggtgagtc
    15061 gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc aatggccagc agcgcattaa
    15121 cattaccttt tagtcggatt gcgcacgctg tcgatagcgc cattccaaca aaatcagacg
    15181 aaaaggttat ctggagcgcc tgtacagtta actgtggtag tcgctgcccg ctacgtatgc
    15241 acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa taccggcgat gacaattacg
    15301 acggcctgca ccaggttcgc gcctgtctgc gtggacgttc catgcgtcgc cgtgtctata
    15361 atccggaccg cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa ggcaaatttg
    15421 agcgcattag ctgggaagaa gcctacgaca tcatcgcgac caatatgcag cgcctgatca
    15481 aagagtacgg caacgaatcc atctatctga actatggcac cggtacgctg ggcggcacca
    15541 tgacccgctc ctggcccccg ggaaataccc tggtcgcgcg cctgatgaac tgctgcggcg
    15601 gttatctgaa ccattacggt gactactctt ccgcgcaaat tgcggaaggt ttgaactata
    15661 cctacggcgg ctgggcagat ggtaacagcc cgtcggatat cgaaaacagt aaactggtag
    15721 tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg cggtggggtg acttactatc
    15781 ttgaacaggc acgccagaaa tctaatgccc gcatgatcat catcgatccg cgctataccg
    15841 acaccggtgc cgggcgcgaa gatgagtgga tccctattcg tccgggaaca gatgccgcac
    15901 tggttaacgg tctggcgtat gtcatgatca ccgaaaaact ggtggatcag gcattcctcg
    15961 ataaatattg cgttggctac gacgagaaaa cactgccagc cagtgcgccg aaaaatggtc
    16021 actataaagc ttatattctg ggtgaagggc cagatggcgt ggctaaaacg ccggaatggg
    16081 cctcgcaaat cactggtgtt ccggcagaca aaatcatcaa attggctcgt gaaatcggta
    16141 gtaccaaacc ggcgtttatc agccagggat ggggcccgca gcgtcacgct aacggtgaaa
    16201 tcgcaacccg tgctatctcg atgctggcga ttctgaccgg taacgttggt attaacggag
    16261 gcaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt tgtccgtatg ccgaccctcg
    16321 aaaacccgat tcagaccagc atttcgatgt ttatgtggac cgatgccatt gaacgtggcc
    16381 cggaaatgac agcgctgcgt gatggtgtac gcgggaaaga taagctggat gtgccgatca
    16441 aaatgatctg gaactatgcc ggtaactgcc tgattaacca gcattctgaa atcaaccgta
    16501 cccatgaaat ccttcaggat gataagaagt gcgagctgat tgtggttatc gactgccaca
    16561 tgacctcatc ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct tccgaacaga
    16621 tggactttgc actggatgca tcctgcggga atatgtctta cgtgattttc aacgatcagg
    16681 tgattaaacc gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac
    16741 gtcttggcgt tgagcaacag tttactgaag gccgtacaca ggaagagtgg atgcggcatc
    16801 tgtatgccca gtcgcgggaa gcgattcctg aactgccaac gtttgaagag ttccgcaagc
    16861 aggggatctt taaaaagcgc gacccacaag ggcaccacgt tgcttataaa gctttccgtg
    16921 aagatcctca ggcaaatcca ctgaccacgc catcgggtaa aattgagatt tattcgcagg
    16981 cgctggctga cattgccgct acctgggaat tgccagaagg cgatgtgatc gatccactgc
    17041 cgatctacac gccgggcttt gaaagttatc aggatccgct gaacaaacag tatccgctgc
    17101 agcttacggg tttccactat aaatctcgcg ttcactcaac ttacggcaac gttgatgtgc
    17161 tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct tgatgcgcaa aaacgcggta
    17221 tcaacaacgg cgataaagtc aggatcttta acgatcgtgg tgaggttcat attgaggcga
    17281 aagtgacgcc acgaatgatg ccgggtgtgg tcgcactggg tgaaggtgcc tggtatgacc
    17341 cggatacaaa acgtgtcgat aagggcggtt gtatcaacgt actgaccact caacgtccgt
    17401 ctcctctcgc taaggggaat ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag
    17461 gagtaaccga tgacaaccca gtatggattt tttattgatt ccagccgttg caccggttgc
    17521 aaaacctgcg agctggcctg taaagactac aaagatttga cgccagaagt cagcttccgc
    17581 cgcatttatg aatatgctgg cggcgactgg caggaagata acggtgtctg gcaccagaat
    17641 gtgtttgcct actatctgtc gatttcatgt aaccactgcg aagatccggc ttgtactaaa
    17701 gtctgcccga gcggtgcgat gcataaacgt gaagatggtt ttgttgtggt cgatgaagat
    17761 gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt atggcgcacc gcaatataac
    17821 gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc ggagggtaaa
    17881 aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg atttcggccc tatcgacgag
    17941 ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc tcactttacc
    18001 aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga taccactggc
    18061 tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat ggccgctgat
    18121 gatcttcacg gtcttcgggc aatgtgtagc aggtggtttt atcgttctgg ctttggcgct
    18181 gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca tgtttggttt
    18241 atgggtgctg atgggcattg gctttatcgc ctctatgctc catcttggtt caccaatgcg
    18301 cgcttttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg
    18361 ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc
    18421 accggcattg cgtacgctgt ggctgatagt gacgatggtt cttggcgtca tctttgtctg
    18481 gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca gcatctggac
    18541 gccgatgggc ttcttcctga cgatgtttat gggcggcccg ttactgggtt acctgttgtt
    18601 gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg ccagcaattt ctgtactggc
    18661 actggtagtg agtggcgtgg tgtcagtgat gcagggcgca gagctggcga ctattcatag
    18721 ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcggat
    18781 cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc
    18841 tgcggttccg ctactttctg tatcattcat tctgcttctg gcaggggaat taatcggtcg
    18901 cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcataaca
    18961 accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc ataactggtc
    19021 atcaaattac gataatcagg aatgtggtta gagaacaacg tcgccagtcc ttcaatatca
    19081 ttacgccagt cgcgatgcag ctcgcacgcc acgccgaacc atgtcatcaa ttgcgcacca
    19141 gcttgcgaca tgcgatccca tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg
    19201 tcagtcacga caaagacgtc aaacccttct tcaatggctg aaagcgccgg gaatgccacg
    19261 caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc tttgacagct
    19321 tttacaaaat cttcgttatc ccaggcgtta atatttcccg ggcgagcaat gtaaggtgta
    19381 tcggggaatt ctgctttcag ttctggaact aatgggccgt taggaccggt ttcaaaactg
    19441 gtggtaagaa tagttggtaa gttgaaatac ttagccaggt cacccagcgc cagcacgtta
    19501 tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc ctggtgatca
    19561 acaagcaaaa cggcagcatc atttttatca agacgaacat acggtttggt catcctcttc
    19621 tcctttcgag agtcagtggt acgtctgaaa tcgatcagac gcgatgcatt gctctgaaag
    19681 catagacggg aaatatgagt ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat
    19741 gaaatttgtg ttagttcaat aaaaacaatc agatgagact aatcatctct tcagcattag
    19801 cgttatgaat gagtaatata aatcgataat accgccgctg ggatatcgcg tattttcacc
    19861 cattgacaat gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc ctcacggttt
    19921 taattcatgt ccacgtatac ccggcctgtc atgcttttgc tgtctggcct gcttttgttg
    19981 actctggcga ttgcggtgtt aaatacactc gtgccgcttt ggctcgccca ggaacacatg
    20041 tccacatggc aggtaggcgt tgtcagctca tcctatttta ccggcaacct tgtcggtaca
    20101 ttgctgacag ggtatgtcat taagcgcatt ggctttaacc gcagctatta tctggcctcc
    20161 ttcatttttg ccgctggctg tgccggcctt ggcctgatga ttggattctg gagctggttg
    20221 gcttggcgtt ttgtcgcggg cgtcggctgt gccatgattt gggtggttgt tgagagcgcg
    20281 ctgatgtgca gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcgta tatgatggtt
    20341 tattacgtgg gaacgttttt aggccagtta ctggtcagca aagtttcaac cgagctgatg
    20401 tccgtattgc cgtgggttac aggtttgacg ttggcaggga tcttaccgct gttgtttacg
    20461 cgtgtgctga atcagcaggc tgaaaaccat gattcgacgt caattacgtc aatgctaaaa
    20521 ctccgtcagg cgcggcttgg cgtgaatggc tgcattatct caggaatcgt tctgggatct
    20581 ctatatggcc tgatgccgct gtacctcaat cacaaagggg tgagcaatgc cagcattggt
    20641 ttctggatgg cggtactggt cagtgcgggt atccttggac aatggccgat tggacgtctg
    20701 gcggataagt ttggtcgact gctggtgttg cgtgttcagg tctttgtcgt cattctcggc
    20761 agtatcgcga tgcttagcca ggcggcgatg gccccagcgt tattcatcct cggtgccgct
    20821 ggctttacgc tatatccggt ggcgatggca tgggcttgcg agaaagttga acatcatcaa
    20881 ctggtggcga tgaaccaggc cttactgttg agctatactg tgggaagtct gcttggcccg
    20941 tcatttaccg ctatgctaat gcagaatttc tccgataatt tattgtttat catgatcgcc
    21001 agcgtatcgt ttatctattt gctgatgctg ctgcgcaacg ccggtcatac gccgaaaccc
    21061 gttgctcacg tgtaaat
//
LOCUS       DAJSKJ010000058        20750 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000058 DAJSKJ010000000
VERSION     DAJSKJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.058"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(193..1284)
                     /gene="ychF"
                     /locus_tag="ON345_001851"
     CDS             complement(193..1284)
                     /gene="ychF"
                     /locus_tag="ON345_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR3071918.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1401..1985)
                     /gene="pth"
                     /locus_tag="ON345_001852"
     CDS             complement(1401..1985)
                     /gene="pth"
                     /locus_tag="ON345_001852"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3071919.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2263..2541
                     /gene="ychH"
                     /locus_tag="ON345_001853"
     CDS             2263..2541
                     /gene="ychH"
                     /locus_tag="ON345_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR3071920.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2596..4275)
                     /gene="dauA"
                     /locus_tag="ON345_001854"
     CDS             complement(2596..4275)
                     /gene="dauA"
                     /locus_tag="ON345_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR3071921.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4400..5347)
                     /gene="prs"
                     /locus_tag="ON345_001855"
     CDS             complement(4400..5347)
                     /gene="prs"
                     /locus_tag="ON345_001855"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3071922.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5498..6349)
                     /gene="ispE"
                     /locus_tag="ON345_001856"
     CDS             complement(5498..6349)
                     /gene="ispE"
                     /locus_tag="ON345_001856"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR3071923.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6349..6972)
                     /gene="lolB"
                     /locus_tag="ON345_001857"
     CDS             complement(6349..6972)
                     /gene="lolB"
                     /locus_tag="ON345_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR3071924.1"
                     /translation="MPLPDFRVIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7186..8442
                     /gene="hemA"
                     /locus_tag="ON345_001858"
     CDS             7186..8442
                     /gene="hemA"
                     /locus_tag="ON345_001858"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR3071925.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8484..9566
                     /gene="prfA"
                     /locus_tag="ON345_001859"
     CDS             8484..9566
                     /gene="prfA"
                     /locus_tag="ON345_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR3071926.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAESRRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9566..10399
                     /gene="prmC"
                     /locus_tag="ON345_001860"
     CDS             9566..10399
                     /gene="prmC"
                     /locus_tag="ON345_001860"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3071927.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10396..10788
                     /locus_tag="ON345_001861"
     CDS             10396..10788
                     /locus_tag="ON345_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR3071928.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10792..11601
                     /locus_tag="ON345_001862"
     CDS             10792..11601
                     /locus_tag="ON345_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR3071929.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11637..12491
                     /gene="kdsA"
                     /locus_tag="ON345_001863"
     CDS             11637..12491
                     /gene="kdsA"
                     /locus_tag="ON345_001863"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR3071930.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12640..12747)
                     /locus_tag="ON345_001864"
     CDS             complement(12640..12747)
                     /locus_tag="ON345_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR3071931.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13175..13282)
                     /locus_tag="ON345_001865"
     CDS             complement(13175..13282)
                     /locus_tag="ON345_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR3071932.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(13687..14787)
                     /gene="chaA"
                     /locus_tag="ON345_001866"
     CDS             complement(13687..14787)
                     /gene="chaA"
                     /locus_tag="ON345_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR3071933.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15057..15287
                     /gene="chaB"
                     /locus_tag="ON345_001867"
     CDS             15057..15287
                     /gene="chaB"
                     /locus_tag="ON345_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR3071934.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15445..16140
                     /gene="chaC"
                     /locus_tag="ON345_001868"
     CDS             15445..16140
                     /gene="chaC"
                     /locus_tag="ON345_001868"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR3071935.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLELDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16184..16537)
                     /gene="ychN"
                     /locus_tag="ON345_001869"
     CDS             complement(16184..16537)
                     /gene="ychN"
                     /locus_tag="ON345_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR3071936.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16721..18115
                     /gene="ychO"
                     /locus_tag="ON345_001870"
     CDS             16721..18115
                     /gene="ychO"
                     /locus_tag="ON345_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR3071937.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     AMQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18116..18766)
                     /gene="narL"
                     /locus_tag="ON345_001871"
     CDS             complement(18116..18766)
                     /gene="narL"
                     /locus_tag="ON345_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR3071938.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18759..20555)
                     /gene="narX"
                     /locus_tag="ON345_001872"
     CDS             complement(18759..20555)
                     /gene="narX"
                     /locus_tag="ON345_001872"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR3071939.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRKSGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         5195 a         5203 c         5348 g         5004 t
ORIGIN      
        1 agatatccgt catctgacac tgatgtcttt aattattctc gtggttcact gtaggcaata
       61 agcacaaaag tgtaggatgt tacaagaatg attaggactc ggtgaaatga aaaatccacg
      121 caattgcgtg gattatatat acttttgcgc tcttcatgag atttagcgaa acctcatgag
      181 acaacaaatt aattagacgt tgaagaggaa gttcatcaca tcgccatctt taacgatgta
      241 atctttacct tctgcacgca ttttgcctgc ttctttcgcg ccttgttcac ctttgtaagt
      301 gatgaaatct tcaaacgaga tggtttgtgc acggataaag cctttttcaa aatcagtatg
      361 gattttgccc gctgcctgcg gcgcggttgc tccaaccgga atggtccatg cacgcacttc
      421 tttcacccca gcggtgaagt aagtttgcag gttcagcagc ttataaccgg cacggatcac
      481 acggttcagg cccggctctt ccagcccaag ctcctgcata aactcgtcac gttcttcgtc
      541 gtccagttcg gcaatgtctg cttcaacagc agcacaaacc ggaaccacaa cagaaccttc
      601 tttcgccgcg atttcacgca cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt
      661 gacgttggcg atgtacattg ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc
      721 tttctcttca gcgcttaaat ccagcgcgcg cagcatacct gcattttcca actggggcag
      781 gcatttttcc aggaccgcca gctcagcttt cgcgtcttta tcgccacctt tggctttctt
      841 ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt
      901 gataacttca atatcgtcag ccgggttaac tttgccggaa acgtgaatga tgttgtcatt
      961 ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg tcaggaactg
     1021 gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc
     1081 catggtcgtg ggaagcgtac gttgcggttt tacgatttca gccagttgat ccaggcgagg
     1141 gtcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc
     1201 ttcaataccg gctttggtca gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc
     1261 gacgataccg catttgaatc ccatgattta aatcacctta atatcttaat aatcaacctg
     1321 ttattgctaa cagattgcag aaatggaaat aactttgcct attatacacg gcactcggca
     1381 aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg
     1441 gtcaagccat ctgtaaacca catttcagta caacgcgccg cttcgtcgat ggcttcatca
     1501 attaacttct gttcactaac aggcggtttg cctaacacaa aaccgacaac tttattttta
     1561 tcgcccggat gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt acccaattta
     1621 ctgatgatgt ctttcagtcc attgtgacca ccatggccac cgcccaattt aaatttggcg
     1681 acgccaggag gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg
     1741 aaaaaactgg ccatcgccgc aacggctttg ccgctgagat tcataaatgt agtcgggact
     1801 aacaggcgga catcttcgcc tccaagagtg actcgcgaag tataaccaaa gaatttagcc
     1861 tcttcgcgca gcggagcgcg caaacgctct gccagtaagt caacgaacca ggcaccagca
     1921 ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat caatttaatc
     1981 gtcacgtttt tttgtcctga gtgtgtacat aactggcgcg tagtttactg gttgcggccc
     2041 cgcttgacaa aaaactgcgt atcaaatgca gataacgtaa taattgcctg agtggactat
     2101 tagaaagtca aggtgttcag gcgtttattt gtaaagtttt gttgaaataa gggttgtaat
     2161 tgtgatcacg cccgcacata acccacaggg tgttgtctat actttacaca taaggaagag
     2221 gggtattccc tgttacaacc cagaaagttc cggaggtgac atatgaaacg caaaaacgct
     2281 tcgttactcg gtaacgtgct catggggttg ggtctggtgg taatggtggt cggcgtgggg
     2341 tattcaatcc tcaaccagtt accacagttt aatatgcccc agtatttcgc gcatggtgca
     2401 gtgctaagta ttttcgtcgg tgccattctc tggctggcgg gtgcccgtgt tggcgggcat
     2461 gaacaggtgt gcgaccgtta ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc
     2521 gataatcgcc gtcatagcta acaaataatg cagtttgctg accagtcagt ttgcgctgac
     2581 tggtcaatct cgtacttata aatccgccat cgccgcgcga cgattcggga agaacgcgag
     2641 gcgtcccggg atcggttgaa tgcctgcgcg agccatagtg cgcagtggct ggaattccac
     2701 gttgcacacg cgcagttcac atccttcggg cagacgcttc acaaaacgct ggaacgcatc
     2761 aagaccacca gcatcaagta ccggaacggc atcccacttc agaatcacaa tccgtttgcc
     2821 ttcaagacgt gactccaggt ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc
     2881 aataacgcgc agtaccagaa catcgtctgg aacatctacg actaccggtg ccaggcgagt
     2941 catacgtgcg atacgacgca taaacagcag cgatgccagc acgatcccca cgctgatggc
     3001 aataaccata tcaaacagca cggtcagcga catgcacagc agcatgacaa tgatgtcatc
     3061 tttcggtgca tgacgcagca agtcgaccac tttatgcgct tcactcatgt tccacgccac
     3121 catcaacagc agggctgcca tagcggaaag cggcagccag gagagcagcg gtgccagtac
     3181 cagcagggca agaataacca gaatagagtg gatcaccgct gagatagggg aagttgcccc
     3241 ggcacggacg ttagcggcag aacgcgcgat ggcagctgta gcggtaatac caccaaagaa
     3301 cggagcgata atattcccca gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt
     3361 cccggtcata ccatccagta ccacggcgca gagcagagat tcgattgcgc cgagcattgc
     3421 cattgagaat gccgcaggca gcagtgtgcg aatagaatcc caggttagcg tgaattctga
     3481 attaggcaga tcccacggca gcaccagttg cggcagcagt tgcggaatac cgttaccctg
     3541 agaaccatcg gccaggacgt agtggaattg cgaaccgatg gtagcaacat gtccgccgag
     3601 caggttaaca atccccatca ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa
     3661 acgaatgccc agacgcggcc aaaaaacaag aatacctagc gtcacaatgc caatggcagc
     3721 atcacccaca ttaatggtcg gcagcgccat aaataatgcg ccgacttttt gtagataatg
     3781 ttccgggaca tgggccattt gcagaccgag aaaatcttta atctgcatgg taccgatggt
     3841 gatcccgata cccgaggtga aacctaaggt gacggaaacc ggaatatact caatcaggcg
     3901 accaaagcgt gccagaccca tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag
     3961 cagtcctgcc agtccaaact gttgcgacac gggatagaga attaccacaa atgccgcagt
     4021 cggaccggaa acgctaaagc gtgacccacc cgtcagagca atgacaatcc ccgcaacagc
     4081 tgcggtatat aaaccgtact ggggtgccac accactacca atagccaacg ccatcgccag
     4141 cgggatagca ataatcccga cggttatccc ggcaatcagg tcacgggtaa accgtgcggc
     4201 agtatatttt tctttccagc aagcgtcgat cagagcgcgg aaaggcatca catgtgagga
     4261 aaatattttg ttcacaataa tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca
     4321 ggtgaaggag gcataggtca tacaaatgga tattacagac aaaaaaaccc gccgcagcgg
     4381 gtctttgagc cgggttcgat tagtgttcga acatggcaga gatcgattct tcgttgctga
     4441 tacgacgaat cgcttcggcc agcatacctg acagggtcag agtacgcacg ttcggcagtg
     4501 atttgatttc atcgctcagc ggaatggtat cgcagacaac gacttcatca attacagagt
     4561 tacgcaggtt gttcgccgcg ttgccagaga agatcgggtg agtcgcgtac gcaaatacac
     4621 gtttagcacc acgttctttc agagcttcag cagctttaca cagcgtaccg ccagtgtcga
     4681 tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc accgatgata tgcatcacct
     4741 gggaaacgtt cgcacgcgga cgacgtttgt cgatgattgc catatcggta tcgttcagca
     4801 gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc cggagaaacc acaattgggt
     4861 tatccagatt cagctgcagc atgtcttcca gcaggatcgg gctaccaaat acgttatcaa
     4921 ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag atccactgtc agcacacggt
     4981 caacaccgac gctggagagg aagtctgcaa ccactttcgc agtgattggt acacgagcgg
     5041 aacggacgcg acggtcctgg cgcgcatagc caaagtaggg gataacagcg gtgatacgac
     5101 ctgcggaagc acgacgcagg gcatcaacca taacgactaa ttccatcagg ttgtcgttag
     5161 taggggcaca agtggactgg atgatgaaaa tatcaccacc gcgtacattt tcattaattt
     5221 gtacgctgac ttcgccatcg ctaaagcgac ctacagcggc gtcgccgagt gaagtgtaca
     5281 ggcggttggc aatacgttgt gctagttccg gggtggcgtt accagcaaaa agcttcatat
     5341 caggcacgag aagaacctca ggcatgcgtc cattggtgga aagaatctgc cgaaaactgt
     5401 gcgggccagg catgatcctt tccaggcggt gtattaaaga gcgcgatgca acgtctggaa
     5461 caaggtgacg ttgtcaccga aactcagctt gcccggctta aagcatggct ctgtgcaatg
     5521 gggaaagatt aacgcctttc gccacaaagc cattgagcca ttccggggct tgctctagca
     5581 cctggcgggc ttcagactct gtatcaaatt cagcaaagac acaggcccct gtcccagtca
     5641 ggcgcgacgg ggcgtattct aacagccagg aaagcaccgc atcaacctcg cgaaaacgtt
     5701 ttcttgcgat aacctcgcaa tcattgctga attcacattt tagcaacgtt tctattgacc
     5761 tttttggcgt attgcgcggg agttcaggat ctttaaaaat caccggagtc ggaatactta
     5821 caccagggtg cgccaccaga taccacttct ctggcggatc caccggcgtt agtatttcac
     5881 caacgccttc ggcaaacgcg gcatgccccc gaacaaagac aggaacatct gcgcccagcg
     5941 tcagccccat ttccgccagc tcatccatgc ttagcccgca ttgccagaga tgatttaatg
     6001 ccaccaggac cgtcgcggca ttggatgaac caccgccgag accgccgccc atcggtaaac
     6061 gcttgtcaat gctgatattc gcaccgcttc ccgtcggaag acgcccgctg tctgccgcag
     6121 ttttcatcaa caaccgcgct gcgcgaacga tcaggttatc ttcatgttcc acgccttcaa
     6181 cgggcgttaa cagacgaata tccccatcgt cacgaagctc aatgctgatg gtgtcgccgt
     6241 aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc atccgcacgc tgaccggtaa
     6301 tgtataaaaa cagattaagt tttgccggag agggccactg tgtccgcatt atttcactat
     6361 ccagttatcc atttttaact tgatgcgttg accaccgtcg gtgagttcca tattggctgg
     6421 catcgcaggt tgcgttttgg tgtcataacc accataaaca accttccagt ttttgccatt
     6481 ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc agtttgtagt cggttgcatc
     6541 acccggtaaa cctaaaatcc actggcgcaa gctgttgagc ggaattggca ttccggtcaa
     6601 tttgccaatc atctcttcgg cgtcatcggc ggtataacgc tgacctttat tgtcgactaa
     6661 ctgcacgtta cccggttgag cattcagctc cagttccgtg ctgcccaatg ggttagtgag
     6721 cagcagacgg tagcgatcct ggccggtttg ctgccagaaa aagcgggcgt acactttttg
     6781 ttggtcagaa atataagcga acgcgccgcg agtctgatac tgattaagat tgcgcacgtc
     6841 ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg ccaggacctt tgggcgtggt
     6901 aacggaacag gcagtaagca caagagcagc cagcggtagc aggcggataa cacgaaaatc
     6961 gggcaggggc atagtgatga caagtccttg agatacgttg cagttataac ccttaatgct
     7021 agcgttaccg tccgctatcg tctatgttca agttgtctta attgccagaa tctaacggct
     7081 ttcggcaatt actccaaaag ggggcgctct cttttattga tcttacgcat cctgtatgat
     7141 gcaagcagac taaccctatc aacgttggta ttatttcccg cagacatgac ccttttagca
     7201 ctcggtatca accataaaac ggcacctgta tcgctgcgag aacgtgtatc gttttcgccg
     7261 gataagctcg atcaggcgct tgacagcctg cttgcgcagc cgatggtgca gggcggcgtg
     7321 gtgctgtcga cgtgcaaccg cacggaactt tatcttagcg ttgaagagca ggataacctg
     7381 caagaggcgt taatccgctg gctttgcgat tatcacaatc ttaatgaaga agatctgcgt
     7441 aaaagcctct actggcatca ggataacgac gcggttagcc atttaatgcg tgttgccagc
     7501 ggcctggatt cactggttct tggggagccg cagatcctcg gtcaggttaa aaaagcgttt
     7561 gccgattcgc aaaaaggtca tatgaaggcc agcgaactgg aacgcatgtt ccagaaatct
     7621 ttctctgtag cgaaacgcgt tcgcactgaa acagatatcg gtgccagtgc tgtgtctgtc
     7681 gcttttgcgg cttgtacgct ggcgcggcag atctttgaat cgctctctac ggtcacagtg
     7741 ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc gtcatctgcg cgaacataaa
     7801 gtacagaaga tgattatcgc caaccgcact cgcgaacgtg cccaaattct ggcagatgaa
     7861 gttggcgcgg aagtgattgc cctgagtgag atcgacgaac gtctgcgcga agccgatatc
     7921 atcatcagtt ccaccgccag cccgttaccg attatcggga aaggcatggt ggagcgcgca
     7981 ttaaaaagcc gtcgcaacca accaatgctg ttggtggata ttgccgttcc gcgcgatgtt
     8041 gagccggaag ttggcaaact ggcgaatgct tatctttata gtgtggacga tctgcaaagc
     8101 atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg ttgaggcgga aactattgtc
     8161 gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac aaagcgccag cgaaaccatt
     8221 cgcgagtatc gcagccaggc agagcacgtt cgcgatgagt taaccgccaa agcgttagcg
     8281 gcccttgagc agggcggcga cgcgcaagcc attatgcagg atctggcatg gaaactgact
     8341 aaccgcttga tccatgcgcc aacgaaatca cttcaacagg ccgcccgtga cggggataac
     8401 gaacgcctga atattctgcg cgacagcctc gggctggagt agcagtacat cattttcttt
     8461 ttttacaggg tgcatttacg cctatgaagc cttctatcgt tgccaaactg gaagccctgc
     8521 atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc gcaaactatc gccgaccagg
     8581 aacgttttcg cgcattatca cgcgaatatg cgcagttaag tgatgtttcg cgctgtttta
     8641 ccgactggca acaggttcag gaagatatcg aaaccgcaca gatgatgctc gatgatcctg
     8701 aaatgcgtga gatggcgcag gatgaactgc gcgaagctaa agaaaaaagc gagcaactgg
     8761 aacagcaatt acaggttctg ttactgccaa aagatcctga tgacgaacgt aacgccttcc
     8821 tcgaagtccg agccggaacc ggcggcgacg aagcggcgct gttcgcaggc gatctgttcc
     8881 gtatgtacag ccgttacgcc gaatcccgcc gctggcgggt agaaatcatg agcgccagcg
     8941 agggtgaaca tggtggttat aaagagatca tcgccaaaat tagcggtgat ggtgtgtatg
     9001 gtcgtctgaa atttgaatct ggcggtcatc gcgtgcagcg tgttcctgct acggaatcgc
     9061 agggtcgtat tcatacttct gcttgtaccg ttgcggtaat gccagaactg cctgacgcag
     9121 aactgccgga catcaaccca gcagatttac gcattgatac tttccgctcg tcaggggcgg
     9181 gtggtcagca cgttaacacc accgattcgg caattcgtat tactcacttg ccgaccggga
     9241 ttgttgttga atgtcaggat gaacgttcac aacataaaaa caaagcgaag gcgctttcag
     9301 tgctcggtgc tcgcatccac gctgctgaaa tggcaaaacg ccaacaggcc gaagcgtcta
     9361 cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg taaccgtact tacaatttcc
     9421 cgcaggggcg cgttaccgat caccgcatca acctgacgct ctaccgcctg gatgaagtga
     9481 tggaaggtaa gctggatatg ctgattgaac cgattatcca ggaacatcag gccgaccaac
     9541 tggcggcgtt gtccgagcag gaataatgga atatcaacac tggttacgtg aagcaataag
     9601 ccaacttcag gcgagcgaaa gcccgcggcg tgatgctgaa atcctgctgg agcatgttac
     9661 cggcagaggg cgtactttta ttctcgcctt tggtgaaacg cagctgactg acgaacaatg
     9721 tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt gaacccattg ctcatttaac
     9781 cggggtgcga gaattctggt cgttgccgtt atttgtttcg ccagcgacct taattccgcg
     9841 cccggatacg gagtgtctgg tggagcaggc actggcgcgg ttgcctgaac aaccttgccg
     9901 tattcttgat ctcgggacgg gtaccggggc gattgcgctt gcgctggcta gcgagcgccc
     9961 ggactgcgaa attatcgctg tagatcgtat gcctgatgct gtctccctgg cacaacgtaa
    10021 tgcccagcat ctggcgatca aaaatatcca cattctgcaa agcgactggt ttagcgcgct
    10081 agccgggcag cagtttgcga tgattgtcag caatccgccg tatattgacg agcaggaccc
    10141 acatcttcaa caaggcgatg tccgctttga gccgctcact gcgctggttg cggcagacag
    10201 tggaatggca gacatcgtgc atatcatcga acagtcgcgt aacgcgctgg tatccggcgg
    10261 ctttctgctt ctggaacatg gctggcagca gggcgaagcg gtgcgacaag catttatcct
    10321 cgcggggtat catgacgtcg aaacctgccg tgactatggt gataacgagc gcgtaacgct
    10381 cggccgctat tatcaatgac aagtttttct acactgctta gtgttcatct tattagtatc
    10441 gcgctttctg ttgggctatt aaccttacgt ttctggctac gttatcagaa gcatccacag
    10501 gcatttgctc gctggacgcg cattgtgccg ccggttgtcg atacggtgtt attgttaagc
    10561 ggcattgcgt tgatggctaa agcgcacatc ctgccatttt ccgggcaggc acagtggctg
    10621 actgaaaagc tgtttggagt tatcatttat atcgttttgg gttttattgc actcgattat
    10681 cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc cgctggcgtt ggtggtgctg
    10741 tacatcatca ttaaactcgc caccacaaaa gtaccgttac tggggtaagt catgagatcg
    10801 ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag gcatgatcct ggcttgcgaa
    10861 gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg aactggagcg tctcgttagt
    10921 ctggcgaagg aagaaatcag ccagcttctg cctttagaag agcagctgga aaaactgatc
    10981 gcgctgtttt acggcgactg gggatttaaa gcctcacgcg gcgtttatcg tctttccgat
    11041 gcattatggc tggaccaggt gttaaagaat cgacagggca gtgcggtatc attaggtgcg
    11101 gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat tttccctacg
    11161 cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa tccttttaac
    11221 ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat cagcccgtcg
    11281 gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg
    11341 ctggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc gttacgcacc
    11401 agcgaagctt tattacaatt caaccctgaa gatccctatg aaattcgcga tcgcgggttg
    11461 atttatgcgc aactggattg cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa
    11521 cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa catcgcgcat
    11581 aaacatattg tgctgcatta attaatcgac attttactca agattaaggc gatcctatga
    11641 aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgccg ttcgtactgt
    11701 ttggcggtat gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc gagcactacg
    11761 taactgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt gacaaagcca
    11821 accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg aaaatcttcc
    11881 aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg
    11941 cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga
    12001 ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt
    12061 ttgtcagccc gggacagatg ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa
    12121 aagtgattct ttgcgatcgt ggtgctaact tcggctatga caacctggtt gtcgatatgc
    12181 tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc
    12241 acgcactgca atgccgcgat ccgtttggcg cagcttccgg tggtcgtcgt gctcaggtgg
    12301 ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt gaagcgcatc
    12361 cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct aaactggaac
    12421 cgttcctcaa gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa gaactggata
    12481 ccagcaagta atcttttttg cttgaaaaat aaagtcttag cgttctgcgt taagactttt
    12541 ttcatgggtg ccagatacaa aaaaggccgc aggctgttac ccctgcggcc ggtttcgggc
    12601 gcatattgcc atcacggcag cctgacgccc gttttcacct tacttccggt tacgccacca
    12661 gccgacaatc gctgcggtaa taattcccgc caggatcggt gctgccaggt cgtgccagaa
    12721 aatcatggca aactgcgcga gcgtcatata gccgccttgt tgtaatgaca acattttgcg
    12781 gctattcttg aattgttctg gttcaagatt agcccccgtt ctgttgtcag gttaaacctc
    12841 tcaacgtgcg ggggttttct ctttccagca accaatgcca ccagggataa agcccccgca
    12901 acattgcgcc tcaccggata acgccggctt ggtgtggata atacgtctca attcatcttc
    12961 acttcatccc tgaaatgttt gcaatgaaga gtgcattccg gtttttcaac agctgttaca
    13021 gtcgtttcat gagtgctctg gatgatgctt ccagctcggg ttgccaatat ttacttgtgg
    13081 aagagataaa gacaaaaatg gccgcaggct gttacccctg cggccggttt cgggcgcata
    13141 ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc caccagctga
    13201 caatcgctgc ggtaatgatc cccgccagga tcggtgctgc caggtcgtgc cagaaagtca
    13261 tggcaaactg cgcgagcgtc atatagccgc cttgttgtaa tgacaacatt ttgcggctat
    13321 tcttgaagtg tctggtttca agattagccc ccgttttgtt gtcaggtttt acctctcaac
    13381 gtgcgggggt tttctctttc cagcaaccaa tgccaccagg gataaagccc ccgcaacatt
    13441 gcgcctcacc ggataattcc ggcttggtgt ggatactacg tctcaattca tcttcacttc
    13501 atccctgaaa tgtttgcaat aaagagtaca ttccggcttt tcaacagctg ttgcagtggt
    13561 tttgtgcagg ttgtggtaca ggctcgcaat tctgattaac gagctgtcca ggtagtatga
    13621 agaaatacaa taagcaggaa tttatcttat ctcccccgat ttccgtcatc ggaggagata
    13681 taactgtcag gcaaatatcg tcatcaaata ggcggcaaac agtgccagat gcgctgcgcc
    13741 attgagcacg ttagtacgtc cggtggagaa ggagatatgg cacagcacta aagaggccac
    13801 catcaccacc atttctggcg caccaagtgc aaactgtaat tcgttacccg tcataaaggc
    13861 aattagcgtg acgacaggta cggtaagcga aatggttgct aacactgaac caaagaacag
    13921 attcatcgcg cgctggacct ggttgttcaa cactgctttt aacgcaccta aaccttccgg
    13981 cgacagaatc aacagagcca ccaggaagcc agtaaaggcg acaggggcat tcatgctgtc
    14041 gagcaatgtc tccagcgggc tggcattcat tttggtgact gcaataacgg caatcagatg
    14101 gataatcaac cagatagcat gccacacgct gctatgggca gacggtttac cgtgatgcgg
    14161 gtcgtcgtca tcactgtcat cttcgtgctc gtagacaaac aaactttgat gcgttttggt
    14221 ctggatcagc aaaaatacgc catacattgc cgcagaaatt aatgctacca gtaatgcctg
    14281 accggttgaa aaattcgccg caggcagagc cattggaaat accagtacga ttatcgccag
    14341 ggggaacagc gcaattaaat actgcttgat accaaacaga ttcatatatt gggtggcaaa
    14401 cttacgaccg cccaacaata atgaaaagcc aaccagccca ccggtaacaa tcataatgat
    14461 tgaatagagc gtatcacgca ttagcgttgg cgcggcgtcg ccggttgcca ttaaagctga
    14521 aatcaaactg acttcaagaa taaccactga aaggctaaga ataagcgaac cgtaaggttc
    14581 tcccaggcga tgggctaata cgtccgcatg acggacaaca ctaaaggcgc tacttaaaat
    14641 accaataagc gcaagaagat tgatggcaat gaccactggt agtgtctggc tgcttcccca
    14701 caggaacagc actaccagcg ccagaaccgg gaaaataagc gaagtctcct tgtggcgggt
    14761 ttttaccgcc tcttgagcat ttgacattat ggttatccct ttgcagatga atttgttgaa
    14821 aatgtaaaaa ataggtagga aaaataacaa aaagtgtctg gatatcggta acattttacg
    14881 aaattttacc ccgctgtcga ttttttactc attggggcat aaaaataagt aatacgttta
    14941 gacaatgttt gttttagcgt ttcttaagaa cagtctgaca cgaaaattct tatgttttgg
    15001 caagagtaga tattgttgac cacacttaat gttcaacttt gtaaatggag tcaacgatgc
    15061 cgtataaaac gaaaagcgac ctgccggaaa gcgtaaagca cgttctaccg tctcatgccc
    15121 aggatatcta taaagaagcg ttcaacagcg catgggatca atataaagat aaagaagatc
    15181 ggcgtgatga cgccagtcgc gaagaaaccg cgcataaagt ggcctgggct gctgtgaagc
    15241 atgaatatgc caaaggggat gatgataaat ggcataaaaa atcgtaaaac cggtagctta
    15301 gttaaagcta ttcgtgcggt gttgccttgc aagtggtccg cggattgcat attgtcccgt
    15361 tagtggtttc aaaatgagcg gtaaaaatgt ccggaagaca ccaaaacgtt gtcgcaggga
    15421 agtatgcagt ggcggaggtg taaggtgata acgcgtgatt tcttgatgaa tgccgattgt
    15481 aaaacggcat ttggtgccat tgaagaatca ctcttatggt cagcagaaca acgggcggct
    15541 tcgctggcgg cgacgctggc ttgtcgacct gatgagggac cggtgtggat cttcggttat
    15601 ggatcgttga tgtggaatcc ggcactggag tttaccgagt cgtgcaccgg tacactggtt
    15661 ggatggcatc gcgcattttg cctgcgcctg accgccgggc gcgggactgc gcaccagccg
    15721 ggacggatgc ttgcactgaa agagggcgga cgcaccacag gcgtcgccta tcgactgcca
    15781 gaagagacgc tggagcagga actaacccta ttgtggaagc gcgagatgat taccggctgt
    15841 tatctgccaa cctggtgtca gcttgaactt gatgatggac gcacagtaaa cgccattgtg
    15901 tttattatgg acccgcgaca tccagaatat gaatctgaca ctcgcgctca ggtcatcgcg
    15961 ccgttgattg cggcggcgag cggtccgctg ggaaccaatg cacaatacct gttttcactg
    16021 gaacaggagc ttatcaaact gggaatgcag gacgatgggc tgaatgattt gctggtatcg
    16081 gtaaaaaaac tgctggcgga gaattatccg gatggtgtgt tacggccggg attcgcctga
    16141 gtaaacttcc ctcatagtgg ggcgtcagac gccccctcaa acattaaaat gtgagcactt
    16201 tatcggctga cagcgtccat tgcgccagtt ccacaagagt accgatttcc accccatcaa
    16261 taagaggaag tgtgctaatc ccgcgcccgt cggtacaggt tttgcacaat ttcaccggta
    16321 cattctgagc ggtaaggatc tccagcattt gctgaatgtt gtagccttcc cctggttttt
    16381 gcccgcgcaa cccggctgtg accgcatcag acatgaggaa cagacgcaga tccagattgc
    16441 tctcctgctc tcgtaacgca atggccagcc gcaagctgtt aaataaggat tcgctcccgt
    16501 aaggtgcgcc attggcaacg atcacgattt tttgcattat ttactcctgt attcagggaa
    16561 ttagacactc atcttctatc ttactgcttc tgcagcgtct gaccaatcgg tcacattttt
    16621 aaggattttc ctgaaagcgc gagaaaatac gacaaaagtt gccagtaatc gttattcttt
    16681 aaggctatgg ttttcattat taccggaagt taccgacgtt ttgagccgtt tcgttcctcg
    16741 cattatcccg ttttatttac tcttgcttgt agcaggcggt acagctaacg cacaatctac
    16801 cttcgagcaa aaagcggcaa atccctttga taataacaat gatggtctgc cggatttagg
    16861 catggctccc gaaaatcatg atggggaaaa acactttgct gaaattgtga aagatttcgg
    16921 cgaaaccagt atgaatgata acgggctgga tactggcgag caggcaaaag ctttcgcatt
    16981 gggaaaagtc cgcgacgcgc ttagtcaaca ggttaatcag cacgtagagt cctggctatc
    17041 accgtgggga aatgccagtg ttgacgtcaa agtggataac gaaggacatt tcaccggcag
    17101 tcgtggaagc tggtttgtgc cgttacaaga taatgatcgt tatctcacct ggagccagct
    17161 tggtcttact cagcaggata atgggttggt gagcaatgtg ggcgttgggc aacgctgggc
    17221 gcgcggcaac tggctggtgg gttataacac tttttatgac aacttgctgg acgaaaatct
    17281 tcagcgagcg ggctttggtg ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt
    17341 ttaccagccg tttgctgcat ggcatgaaca gacagccatg caggaacaac ggatggcgcg
    17401 cgggtatgac ctgacagccc ggatgcgcat gccgttctat caacacctca ataccagtgt
    17461 cagggtagaa cagtattttg gtgagcgtgt tgatttgttt aactctggta cgggttatca
    17521 caatcccgtc gcgttgagtc tgggattaaa ttacacccct gtgccattag tcactgtgac
    17581 ggcccagcat aaacagggtg aaagtggcga aaatcaaaat aacctcggac tgaatcttaa
    17641 ttaccgcttt ggtgtaccgc tcaaaaaaca actttctgcg ggcgaggttg ccgaaagtca
    17701 gtcgttacgt ggtagtcgct atgataatcc gcagcgaaat aatctaccga ctcttgagta
    17761 ccgacagcga aaaacgttaa cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg
    17821 cgaaacagtg ccgctgaaat tacaaatccg cagtcgttac ggtattcggc aactgatttg
    17881 gcagggcgat acgcagatat taagtttgac gccgggcgca caagccaaca gcgcggaggg
    17941 ctggacgctg atcatgcctg actggcagaa cggggaaggg gcgagcaatc actggcgatt
    18001 gtcggtggtg gtggaagata accaggggca gcgtgtctcc tccaatgaga tcacgctaac
    18061 gcttgtcgaa ccgttcgatg cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga
    18121 aaatgcgctc ctgatgcacc cataccgctg cttccacgcg agacttgagc ttcattttct
    18181 tcagcatgtg cttgacgtgc acttttactg tgctttcggt gatatccagg cggcgggcaa
    18241 tcatcttgtt cggcaaaccc tgggcaatca gcttgagaat atcgcgctcg cgtggggtta
    18301 actggttaac atcgcgctca gtagtggcac ggttagcgcg caagctggcg gccagaacag
    18361 gcgttaatgc ttcgcttaat accatttcgc cagcagctgc ctgatgcaat gctttcagca
    18421 gatcttccgg ttccatatct tttaacagat aaccatccgc gccgcgtttc agtgcggtga
    18481 ccacatcttc ttcatggtta gagacgctga ataccacaat gcgccctgag agggactttt
    18541 cgcgcagttt atccagcgtt tccagaccgt tcatgccggg catattgaga tctaacagga
    18601 tcagatcggg atcaagagac tccgccagtt caataccctg ttcgccatta ctcgcttcgc
    18661 caaccacggt gatatctggt gccatactga taagctgttt tacgccagtt cgcagcatcg
    18721 ggtgatcgtc gatcagcagg atagtagccg gttcctgatt actcatgggt atctccttgg
    18781 acgtctgtga aagttttttc gggaataaag gtgaccacca cttcggtgcc acctgattta
    18841 cgacggcgga cgcggcaatc gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg
    18901 ccgtagtgat tgctgcggat ggcattttca ggcacgccgc agccgttatc ctggacggtc
    18961 agtttgacct gattatcgtt ttgcgccacc gtcaccacga cttcactcgc ttgcgaatgt
    19021 ttgagggcgt tacttaatgc ctcacgggca atttgcaaca agtggattgc ctgatgcgaa
    19081 ggcaccaggc gaggcggcaa ttgataatcc agcttcaccg ggaagccaaa tttggcgctg
    19141 tactcttcgc aactcgcctc cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg
    19201 aatgtggtga gcaattcacg caactgcgcc caggatgcat tcagttcgtt acggatctga
    19261 cttaacagtt cgcggctgct ttctggcagc gcatcgccct gcatctgtaa acaactcacc
    19321 tgcatcttca tgcaagagag agattgggca atagaatcat gcagttcgcg cgcaatggtg
    19381 gcacgctctt ccatcacgat caactgttgc tgacgttcct gatggcgatc cagcgccagc
    19441 gtggcggtga gttgttcaac cagggtatcc accagttgtt gttgatcatg gctaagatga
    19501 cgcccctgcg gcagggtcgc cagcaaaata ccgtactgcg tatgagagtc agccagccgc
    19561 cacttcaggg ttgtgccgcg atcaccaacg ggtaatacgc cgcgcgggca gagctggcag
    19621 cctttatcat cacaagtcat atctggttgg caggtaaact cctgatgatt ctcttcatca
    19681 tcagtatcat aaacccgcag ttcgatatca cgtagcaggg ttaaattctg taagccgttg
    19741 agtacaggtg acaggcgttc acacagcggg gcttgggaat gcaaacggcg gttagcctgc
    19801 cataaaaaag agaggatctg atttttatgc tcaagcccgg cggttttctc ctgaacccgc
    19861 tgctcaagta cggcataact ttcggccagt tctgcagaca tattgttcaa cgcagttcca
    19921 agcatcgcca tttcgttgcg cccgctgatg tttgcgcgtt gggtaaaatc gcgatgactg
    19981 acggcactcg ccattgccag cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag
    20041 ataatagtga acaccagtaa aagtgccata aataccgcca ttacccgatg gaccagtacc
    20101 actgtctcga tgcgcatttc cgtggtgcgg tcaaaaccag ataccagttg atcaagcccg
    20161 gcaacaaact ggctgacatc cgctgacacc gtttctcggt tttgtgcacg catcaacgca
    20221 gggatcagtt cattacgcca gtaatcttgt aaaccctgta attgcgccag ttgtccgtct
    20281 cgttctgctg ctcgagtcaa ctcggcgcta aatgccgttt gttccatctc tttaattaag
    20341 ggcttgtctt tctcgcttaa tggcactgct gccaacagac ggtaactttg catgcgcagc
    20401 gatcccgctt tgttgatcgc atgggcgctg ccctgaacac cttgcaccag ccagccagaa
    20461 accgccatcc ctgccagtcc aatagcagta gaaagcaaca caataagcgc aacctgatta
    20521 accagggtga gcggagagag acaacgttta agcatgtaaa cctcttcctt caggctttaa
    20581 atgagcaata accttaatga atgtgacgat acattctgga atggcagtat tctcggctat
    20641 tggctgaagt atacccatac ccggaaagag ttactcctta tttgccgtgt ggttagtcgc
    20701 tttacatcgg taagggtagg gattctacag caccgtgaaa aatctcataa
//
LOCUS       DAJSKJ010000059        20651 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000059 DAJSKJ010000000
VERSION     DAJSKJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.059"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            74..850
                     /gene="exuR"
                     /locus_tag="ON345_001873"
     CDS             74..850
                     /gene="exuR"
                     /locus_tag="ON345_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR3071940.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1195..1857
                     /locus_tag="ON345_001874"
     CDS             1195..1857
                     /locus_tag="ON345_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR3071941.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1861..2244
                     /gene="mzrA"
                     /locus_tag="ON345_001875"
     CDS             1861..2244
                     /gene="mzrA"
                     /locus_tag="ON345_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR3071942.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2391..2759
                     /locus_tag="ON345_001876"
     CDS             2391..2759
                     /locus_tag="ON345_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR3071943.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2797..3102
                     /locus_tag="ON345_001877"
     CDS             2797..3102
                     /locus_tag="ON345_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR3071944.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="ON345_001878"
     CDS             3105..3509
                     /locus_tag="ON345_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3071945.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="ON345_001879"
     CDS             3499..3798
                     /locus_tag="ON345_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR3071946.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATDAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3985..4377
                     /locus_tag="ON345_001880"
     CDS             3985..4377
                     /locus_tag="ON345_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR3071947.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4447..5433
                     /gene="yqjG"
                     /locus_tag="ON345_001881"
     CDS             4447..5433
                     /gene="yqjG"
                     /locus_tag="ON345_001881"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR3071948.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5726..6091
                     /locus_tag="ON345_001882"
     CDS             5726..6091
                     /locus_tag="ON345_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR3071949.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6333..6689
                     /locus_tag="ON345_001883"
     CDS             6333..6689
                     /locus_tag="ON345_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR3071950.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFCSN"
     gene            complement(6740..7636)
                     /gene="yhaJ"
                     /locus_tag="ON345_001884"
     CDS             complement(6740..7636)
                     /gene="yhaJ"
                     /locus_tag="ON345_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR3071951.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7741..8442
                     /locus_tag="ON345_001885"
     CDS             7741..8442
                     /locus_tag="ON345_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR3071952.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8465..8629
                     /locus_tag="ON345_001886"
     CDS             8465..8629
                     /locus_tag="ON345_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3071953.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8841..10151)
                     /locus_tag="ON345_001887"
     CDS             complement(8841..10151)
                     /locus_tag="ON345_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR3071954.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQLACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10179..11510)
                     /locus_tag="ON345_001888"
     CDS             complement(10179..11510)
                     /locus_tag="ON345_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR3071955.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFHEATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11785..13149)
                     /gene="tdcG"
                     /locus_tag="ON345_001889"
     CDS             complement(11785..13149)
                     /gene="tdcG"
                     /locus_tag="ON345_001889"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR3071956.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSITRYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13221..13610)
                     /locus_tag="ON345_001890"
     CDS             complement(13221..13610)
                     /locus_tag="ON345_001890"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR3071957.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13624..15918)
                     /gene="tdcE"
                     /locus_tag="ON345_001891"
     CDS             complement(13624..15918)
                     /gene="tdcE"
                     /locus_tag="ON345_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR3071958.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15952..17160)
                     /gene="tdcD"
                     /locus_tag="ON345_001892"
     CDS             complement(15952..17160)
                     /gene="tdcD"
                     /locus_tag="ON345_001892"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR3071959.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMAGVADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17186..18517)
                     /gene="tdcC"
                     /locus_tag="ON345_001893"
     CDS             complement(17186..18517)
                     /gene="tdcC"
                     /locus_tag="ON345_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR3071960.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18539..19528)
                     /gene="tdcB"
                     /locus_tag="ON345_001894"
     CDS             complement(18539..19528)
                     /gene="tdcB"
                     /locus_tag="ON345_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR3071961.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19627..20565)
                     /gene="tdcA"
                     /locus_tag="ON345_001895"
     CDS             complement(19627..20565)
                     /gene="tdcA"
                     /locus_tag="ON345_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR3071962.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVESLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5310 a         5401 c         4925 g         5015 t
ORIGIN      
        1 tatatgacca atggcatacc catgaataaa gtggtatgat aacttcgtcc gcattccact
       61 ccgggagacg catatggaaa tcactgaacc acgccgtttg tatcaacaac ttgccgctga
      121 cctgaaagag cgcatcgaac agggcgtcta tctggtgggt gataaactgc ctgcagaacg
      181 ctttattgcc gatgaaaaga acgtcagccg tacggtagtt cgtgaagcca tcatcatgct
      241 ggaagttgaa ggctatgtcg aagtgcgtaa aggttcaggt attcatgtgg tttccaacca
      301 gccgcgccat cagcaggcag ctgacaataa tatggaattc gccaattacg gtccgtttga
      361 gttgcttcag gctcgccagc tcatcgaaag taatattgcc gaattcgcgg caactcaggt
      421 aacgaaacag gacatcatga aactgatggc cattcaggaa caggcgcgcg gcgaacaatg
      481 cttccgtgat tccgagtggg atttacagtt ccacattcag gtagccctgg cgacgcagaa
      541 ctccgccctg gcggccatcg ttgaaaaaat gtggacccaa cgtagccata acccgtactg
      601 gaaaaaactg cacgaacaca ttgattcccg taccgtcgat aactggtgtg atgaccacga
      661 tcaaatcctc aaggcgctga ttcgcaaaga tcctcatgct gctaagctgg caatgtggca
      721 gcacctggaa aacaccaaga tcatgttatt caacgaaacc agcgacgact tcgagttcaa
      781 tgccgaccgc tatctgttcg ccgaaaaccc ggtagtgcat ctcgatacgg ccaccagtgg
      841 cagtaaatga aactcctgtc cgacaggcgc tgcgacgcgc ctgttcgctt cgtcggtaag
      901 cgaaaagtat aaagtgtcag cctgtgtaaa tcctctcgcc accctcccct gcattcagca
      961 aaatcagact ccacggacat gtaattttga taacgaacaa cgttgacctt tgttacaatt
     1021 agattcaatt tgaatttatg tttttgaatg ctttcttatc tcacgattta acagggaata
     1081 gttcaggctg tgttgatgta tcaaacccgc agaacatacc aaaacagcaa taacattgcg
     1141 gtagtgcatc ttttaaaacc agcgtggcgt taaccgattc accaggaata atgaatggaa
     1201 cttttgaccc aattgctgca agccctgtgg gcgcaggatt ttgaaaccct ggccaatcca
     1261 tcgatgattg gcatgttgta ttttgtcttg tttgtaattt tgttccttga aaacggcttg
     1321 cttccagcgg cctttttacc gggcgacagt ttactggtat tggtcggcgt gttgattgcg
     1381 aaaggcgcga tgggttaccc gcaaacgatt ctgctgctga ccgttgccgc cagcctcggc
     1441 tgctgggtca gctatattca ggggcgatgg ctgggcaata cccgcaccgt acaaaactgg
     1501 ctatctcatt tacccgcgca ttatcatcaa cgcgcacacc atctttttca taaacacggt
     1561 ttatcggcgc tgttaattgg tcgctttatt gcgtttgtca gaacactgct gccgacgatt
     1621 gccgggttat cagggctgaa taacgcgcgc tttcagtttt tcaactggat gagcggtctg
     1681 ctgtgggtat tgatcctgac aactctgggt tacatgctcg gcaaaacgcc ggtattttta
     1741 aagtacgagg accagctgat gtcatgcctg atgctgctcc cggtggtgct gctggtgttt
     1801 ggcctggcag gttctctggt cgtgttatgg aaaaagaaat atggaaatcg ggggtaaggg
     1861 atgcaaatac ctcgcatgtc gcttcgccag ctagcctggt ccggcactgt tttacttctg
     1921 gtcggcacgc tgttgctggc ctggtccgcg gttcgccagc aagagtctac gctggcgatt
     1981 cgtgccgttc atcaaggcac aacgatgcca gacggttttt cgatctggca tcaccttgac
     2041 gctcatggca ttcctttcaa aagtatcacc cccaaaaacg acactctgtt aattacattt
     2101 gactccagcg accagagcgc cgccgcaaaa gcggtcctcg acagaacatt gccccatggc
     2161 tacatcattg cgcagcagga caataacagt caggctatgc agtggctgac ccggttacgg
     2221 gataactctc atcgcttcgg ataacttcct gttatccgaa acatatcact cactttggtg
     2281 atttcaccgt aactgtctat gattaatgag gcggtggtta acactaccgc ttcgttgtct
     2341 ggacaatcgt tcctttgtaa taggtccgaa tcacaatgga aggttcaaga atgaaatacc
     2401 gcatcgcttt agctgtttct ctctttgctc ttagtgccgg tagttatgcc actaccctgt
     2461 gtcaggaaaa ggagcaaaat atccttaagg agatcagcta tgccgaaaaa caccaaaacc
     2521 agaatcgtat tgacggtctg aataaagccc tgagtgaagt ccgggccaac tgttcagata
     2581 gccagctgcg tgccgatcat cacaagaaaa tcgcaaagca gaaagatgag gtggcggaac
     2641 gccagcaaga tttagccgag gcgaagcaaa aaggcgatgc cgataagatt gccaaacgcg
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg cattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctactactgg cactgattgg cggtatctgg acgctacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gttagcaaac gatcggcagc
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcacaactg
     3541 ctgagccaga tccagcaaca acggctggat ctttccgcca gtcgtcgtga atggctggag
     3601 gcaacagatg cttacgatcg tcgctggaat atgctgctca gtctgcgctc ctgggcgctg
     3661 gtaggcagta gcgtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg tgaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tcctctctgg ccggagccat tccggcctta tccctcaaat
     3841 tttttgaaga tttttgacag ttttccttgc taacaatcat gtttcaccac gtttatgatt
     3901 ctctccatcg acagcaacga cgctaatacc gcgcccttgc acaaaaaaac aatcagcagc
     3961 ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt actggtagcg
     4021 cgcattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac cgcttactct
     4081 ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgcc gctggtgatt
     4141 ctgcttgagt tcggtggtgg tctggcaatc ctgttcggtt tcctgactcg caccacagcc
     4201 ctgtttactg cgggctttac gctgctgacg gcatttttat ttcacagcaa ctttgctgaa
     4261 ggcgtcaact cgctgatgtt catgaaaaac ctgacaattt ctggcggatt cctgctgctg
     4321 gcaattaccg gtccgggcgc gtatagcatc gaccgcctgc tgaataaaaa gtggtaagca
     4381 cgcgctatac ttaacgataa aaaacgaggt ggaagctcct cgtttttgct attggaggag
     4441 agaaaaatgg gtcaactgat tgacggcgtc tggcatgaca cctggtacga taccaaatct
     4501 accggcggta aatttcaacg ttcagcttcc gcatttcgta actggctcac tgccgatggc
     4561 gcacctggcc ccactggcac aggcggtttt atcgcagaga aagatcgtta tcatctctat
     4621 gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca tgcgcaaact caaaggactg
     4681 gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc tggaaaacgg ctggaccttt
     4741 gatgacagtt ttccgggagc aaccggagat acgctctatc aacatgaatt tctgtatcag
     4801 ctttatctcc acgccgatcc acactacagc ggacgagtta ctgttcccgt gctgtgggac
     4861 aaaaagaacc acaccatcgt cagcaacgaa tcagcagaaa tcatccgcat gttcaatacc
     4921 gcgtttgatg cgctgggcgc aaaagcgggt gattactacc caccagccct gcaaacgaaa
     4981 attgacgaac ttaacggctg gatttatgac accgttaaca acggcgtgta taaagctggt
     5041 tttgccacca gccagcaagc ctacgacgag gcggtggcga aagtgtttga atcgctggcg
     5101 cgactggagc agattttagg ccagcatcgt tacctgaccg gcaaccagct aaccgaagcc
     5161 gatattcgcc tgtggaccac gctggtccgt tttgatccag tgtatgtgac ccacttcaag
     5221 tgtgataagc accgcatcag cgattacctt aatctgtatg gcttcctgcg tgatatctac
     5281 cagatgccgg gaattgccga aacagtcaat ttcgatcaca tccgtaatca ttacttccgc
     5341 agccataaga ccatcaaccc tacggggatt atttcaattg gtccatggca ggatctcgat
     5401 gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg caccttacga ggtgcctctg
     5461 ataattagct aagtgcatgt tgttcatgtt ggatgcggcg tgaacgcctt atcaaaccta
     5521 caaaacattt atattcaata tgttgcaatg accgcgcagg ccagataagc gtagcgcatc
     5581 tggcgatttt gcgcttgtca tcagtcttag cgcgccttca cagcacttta ttaattcctt
     5641 gaacgaatat ttactgccat ttcattcacg tctattctta atttgctgct ttaaaaacaa
     5701 gtgattgagc aaaattgagg caaaaatgga ctggtatctg aaagtactaa aaaattatgt
     5761 cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg tttattctgg tcaacatcat
     5821 ctttacgttc gtgctggggc tgctggataa aatgttaggc tggcaacgcg ctggtggcga
     5881 aggcatcctg acgacaatct acggtattct ggtgttttta ccgtggtggg cagttcagtt
     5941 ccgccgcctg cacgacaccg accgctcggc gtggtgggca ctgttgttct taatcccgtt
     6001 tatcggctgg cttattatca tcgtctttaa ctgccaggcg ggtacgccag gcgaaaaccg
     6061 ctttggacca gatccgaaac tggaacctta aaacaaatct gctcgccgga tgtctgactc
     6121 atccggcttt tatcatcatg ttgatgaatg aataatttac taatgtttat ttaaaatatt
     6181 tcaatgtcgt tatataaatt acctataaaa aacaaccatg tatttatctt aaatatcaaa
     6241 acctttataa catccgaaaa agaaagatac gaatagcatt tacacaatac ccggaataga
     6301 ctttcgtcac ttttaattaa agggatgttt ttatgcagtg gtatttagcc gtattaaaaa
     6361 attatgttgg tttctctggt cgcgcacgtc gtaaagagta ctggatgttt actctgatta
     6421 acgccatcgt cggcgctatt atcaatgtca ttcaattgat tttaggttta gagtttccat
     6481 tcctttctct catttacctg gcagcaacaa tcattccggt cattgctctg tgtgttcgtc
     6541 gtctgcacga tactgaccgt tcaggtgcgt gggcgcttct ctatctggta ccgatcattg
     6601 gctggttagt tctgtttgta tttgcctgtt tagaaggcaa ttctggcagc aatcgttacg
     6661 gaaacgatcc taagttttgt tcaaattaat cttagaattg gggcgacatt tcgccccttt
     6721 ttattaacaa taacaaatat tattttccgc taaaaagttt gggaatttcc cgcagacacc
     6781 aggatttcgc ttcccccata ctgtcacgac gccaggccat aataatatcg atctcgctgg
     6841 tcgattccgg gctgacgaca cgcaactgcc cttccgcaat atctttttcg accatcggat
     6901 acggcatcgt cgccacgcca agccccgcca gtaatgcctg acgtttatct tcaatcgtgc
     6961 tcaccgttaa gcgcggctgt ttgtccagca gctgtacggt caacaccggg cgctcacgag
     7021 cggtatccgc taccgcaatt ccacgatatt tcacgcgcgt cacttcagat aacggttccg
     7081 gctcctgatg aatcgggtga tcaggcgcgg caacgtagac gttcattaag gtatagagct
     7141 tgcgcgagtt gatctccgac gaggaacgaa aatgcatatc cggcgcgata acgatatccg
     7201 cccgcccctg ctccagtcgt tcccacgccc ccgccagcac ttcggtgatg attgccagtt
     7261 gggtattggc ttttgccgcc agtttgtcga ttaacgggaa aaaggcaggt gtcggtacca
     7321 gcgcctcggt cacaatggtg agatgcgttt cccaaccgcg cgcgagggct tccgcatcgg
     7381 tagtcagttt atctgctgct tccagcaaaa cgcgccctcg ctccagcaac atcctcccga
     7441 cattggtgaa tttggtgcga tggcccgagc ggtcaaacag caccacatcc agctcttctt
     7501 ccagtttttg catggtgtag ctaagtgcgg aaggcacgcg tcccagctca tccgccgccg
     7561 ccgcaaaact gccccggcga tcgatcgcat ccataacccg tagtgcttcc agcgttaatg
     7621 ccctttcttt ggccatttcg ttctcattca ggaaatttga acataccggg cagaatatct
     7681 ggctaacaat gtagcgtcca gccccttacc ataaaaggaa gtaaagagag gtcaagaatt
     7741 atgattacta cccgaactgc caggcagtgt ggacaagcag actacggatg gttgcaggcc
     7801 cggtatactt tttcctttgg acactacttc gacccgaaat tgttaggcta tgcctccctg
     7861 cgtgtgctta accaggaagt gctggcccca ggtgccgcct ttcagccgcg aacctatccc
     7921 aaagtcgata ttttaaatgt gattctggat ggagaagcag agtatcgcga cagcgaaggc
     7981 aatcatgttc aggccagcgc cggtgaggcg ttgctgctct ctacccagcc gggtgtcagc
     8041 tacagcgaac acaatctcag caaagacaaa ccgttaacac gaatgcagct ttggctggac
     8101 gcctgcccgc agcgagagaa tccgctgatt caaaaactgg cgcttaatat gggcaagcag
     8161 caattaatcg cctcgccaga gggtacgatg ggaagcctgc aattacgcca gcaagtgtgg
     8221 ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt tccagttgca tgggccacgc
     8281 gcgtatttgc aatcgattca cgggaaattt catgcgctta cgcatcatga agagaaagcg
     8341 gcgctgacct gcggtgatgg ggcgtttatt cgtgacgagg ctaacattac gctggttgcc
     8401 gattccccac tgcgcgcttt gctgatagat ttgcctgtct agttgttttt acagggagat
     8461 gatgatgagt aaaaaatcgg ccaaaaagcg ccagccagtg aagcccgtgg tggcgaaaga
     8521 acctgctcgc accgccaaaa attttggcta tgaagagatg ttgagcgagc tggaagccat
     8581 cgtcgcggat gctgaaacgc gtttagccga ggatgaagct accgcgtaaa gacaggttgc
     8641 cggatgcggc gaaaacgcct tatccggcct acaaaatcca acaacattaa cgaattaaac
     8701 aacccgtagg ccggataaga tgcgccagca tcgcatccgg catcaggtac aaaacacaac
     8761 aacatcaaca agttgagcac cccgtaggcc ggataaggtg cgccagcatc gcatccggca
     8821 caatactcaa aacctggcgt ttatctggcc ttgctcgcca taatctcgat aatctgccga
     8881 tccgtttgct gcatcgaatg gcttgctaac gcacacaggt tggcaatcga ctgctcaaca
     8941 tcatgcgcca cgatcccttc attgccggtc acggcggtat catccagcgc cattaacacc
     9001 gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt cgacgcacca
     9061 tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga gatggtttca
     9121 taacgcccat ccaccagcca tgccatcccg gcggcggccc ccattgctgc ggtcgttgcg
     9181 gcacacagcg cagacaaacg cggtaactgg ttatggatgt aaattgcgct caaatgcgaa
     9241 agcatcagcg cacgcgccag ccgttcatca tccgctccga agtgttctgc taccaccacc
     9301 acaggcattg ttgcggtgat cccctggtta cccgagccgg agttactcat agccggaagc
     9361 gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat ggatgaagag
     9421 agatctttcg ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc aatatgcagc
     9481 ccccacttac cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc ggaatcgaga
     9541 ataaagcgga tcgccgcaaa cgggacttca ttgacgaact tcaggatctc agccagcgtc
     9601 gttctggaaa gcaccgtcag cggagactct tgctcgccct ctgccacaca cgccagctgg
     9661 gtaaacacca caccatcgtg cgtctcaata tgcacaatgt tggtatgccc gccgacgata
     9721 gtgacacacg cccacttctc accgttccag actttggcgc gtgagaagag gatttcattg
     9781 caaggttcct ggatcttaac ggagactttc cccgccgcca gcagtgcttt ggcatcggca
     9841 attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttggcatt tccacctaac
     9901 gcccccagcg ccgccgcaat cggcagcccc accattcccg tgccgggaac ggtgacgccc
     9961 agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac cggaccttcc
    10021 agttctgccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt acatcccagc
    10081 gccggtttta cttcctcctg aactgcaagg atgtaacgct gccataacgg atttaaagtc
    10141 gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc caggaacgga
    10201 gaaacacaaa gcaacaaacc agtgacgata atcaggtaca gagacatccc tttgtatttg
    10261 tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac catgccgaaa
    10321 atcgggctac agatagaggt gaagctcaac accggtgcgt tcagtacgat ggcgctccag
    10381 gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa gattttcgtt aatcttctcg
    10441 gcaggcatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc gtgaaagcct
    10501 aaatagacgc caaagaatgc agtcatgaca gcaaagatat tgagaatgac gctgaccact
    10561 ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag cgcggaaata
    10621 ttctgctcat aggcttttac cgcttcgtca tgccccatcg ccagcgtgaa cgacacggcg
    10681 tagaaaaaga cggtgacaaa caaaatgcca aacgcgatat tcattgcccg caatgcttta
    10741 tgccgcgcca cttcaataga tttttcccgc gagcgataag agatcaccat cgggcttaac
    10801 gtctggataa acaaaatcga cgtcagggta aacggcagcg taataatggc gtttttcacc
    10861 agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc caccatcgac
    10921 acgcccagcg ccgcgaccac cagcagcttg gtcagcacca tgccggtcga aattttgaat
    10981 aacaatttct cgccgcgtga agagatcgcc accagaatgc aaatcagtac cagaccataa
    11041 aagggactgt ctgacagcaa cccttccgtc acgccgaagg tatgcagata ggaagcacta
    11101 tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac gaaatagagc
    11161 gcacctaaca ggatgcccca gtttttacct aaataaccgc taatgacact cgggtaatct
    11221 ttacattctg gtgattctgc cagcgtatta ataaacaacc gctgaaacag atacattgcc
    11281 gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc gacctgcacc
    11341 gggagaaaaa caatccccgc gccaatcgcc atcccgatac tcataatcac ccagccggtg
    11401 tcagtactgt cgaatttgat cgcttctcgc cactcgctct cactcattcc agcacgaccc
    11461 gccggggtcg aagcgtctgc aatgacgcct ttattcgatg caatttccat aatttctcgc
    11521 tcaatatttt gtagggctta ttattttttt ccgagccgca tcaaggcgat atgcggtcta
    11581 cgtttttgca ggcgagttat tagaataaag aaatgatacg cgcgaggtca gagaaaatct
    11641 tcacaatcaa gactttgaaa taacggagat ggataagaat tttctactaa attaatcgca
    11701 agagaatatt cattctctat ttgcggcaga tcacaaaaaa aaggtgcaca tttgtgcacc
    11761 caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca gtcctccgcg
    11821 tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg tctcgatcac
    11881 tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg cggcgtttac
    11941 tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct gtccggcaac
    12001 cggatcgcag gtcagcccaa ggttatgctc catcgcgatt tccgccgcat tgcatacctg
    12061 cgccggactg ccgcccagta gttcagttaa ccctgccgcc gccattgaac aggccacgcc
    12121 aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt tatacagcgc
    12181 gccaatagcc cccgctgcca gaaaatagcg ggtaattgac cgctcgttta ccggacgacg
    12241 gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt tagtcggtgc
    12301 cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt acatgttgat
    12361 ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctagaaacca gctgacgacg
    12421 cagcgctacg gcacgacgcg gcacattgag cggaccaggc agcacgcctt cagtgttcat
    12481 cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaccctg cgtcaatttc
    12541 cgctttgctg cgcagcgcta gctcgttgtg catcatcagg ccagatatag acaggccgtt
    12601 gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta cggacgtttc
    12661 gacatcgtgc gacaggccga agtgttcttc ttcgacaata aacccgccgc cgacagagta
    12721 ataggtttta cttaatagcg cttcctgtcc cttccaggca gtgatccgca ttccgttctc
    12781 atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa aatcaacaat
    12841 atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta taaatgcagg
    12901 gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga taatggcgac
    12961 atccgtggca tggcctttgc ccgtcagtga caacgaccca tacagatcga ccacaatatg
    13021 gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt ttccggcatt
    13081 cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa tatcgaatgc
    13141 actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa cgaccttgcg
    13201 accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat ttccagcttc
    13261 acatctttcg gcaaacgcgc aacctgcaca cagctccggg tcgggtaggt cgcctgatgc
    13321 tcatcgaaga actgcttata gacttcattg atggtggcaa aatcattcag atcggtgatg
    13381 aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac gatcgctttg
    13441 acgttttcga ggcttaaacg cgcctgatct tgcacatcag ccgggatctc accggtctgc
    13501 gggcaaaccg gaatttgccc ggaggtgaag accatgctgc ctaaatcaac gccctgaaca
    13561 taagggccga ttgcgcctgg ggcacgttgc gtttcgataa tctttttcat acatcctccg
    13621 gcgtcagagc gcctgggtaa aagtacgtga aataacatcc tgttgctgtt cacgggtcag
    13681 tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat atttttccgg
    13741 gtgctcgatg gcatccagca gcatttcccg attcattacg ttgacgttga ggtgttgacc
    13801 gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccgacaa ggttggtttt
    13861 acgtactgga tcttctttgc ccagcgccgc aggaacgatt gagaaggtgt acgagatccc
    13921 atctttggcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca cggcaccttt
    13981 gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg cgcgacgacc
    14041 gtccggcgta ttaccggttt tctggccgta caccacgtta gaagtgatag tcagaatcga
    14101 ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca taaagcgttc
    14161 aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact gcggatattc
    14221 accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca cggattggtt tcacgcgggc
    14281 atatttgatg gcagacaggg agtccgtcgc caccgacagg cccgcgatgc cgcatgccat
    14341 agtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc tgtacttgtc
    14401 gtgcatgtag tgaatgatat tcagggcgct gatgtactgc accgccagcc agtccatgaa
    14461 gtgatcgagg ctgtccatca ctttgtcgta atccagcacg tcgtccatca gcggtgctgt
    14521 tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg cgtagagcag
    14581 cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa tcaccattgg
    14641 gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca tcagatcgtc
    14701 attttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt ttttgaaggc
    14761 aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgccg gccccatagt
    14821 gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac cgtccagccc
    14881 catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg aatcaaattc
    14941 cggtgtacgc aggaagcgta ccatacggat cttcatgatg aagtgatcga tcagttcctg
    15001 tgcctgctgc tcattgagta cgccagcttt aaagtcgcgc tcaatgtaga tatcgaggaa
    15061 cgatgccgtg cggcccagcg acatcgcgcc gccattttgc gatttcactg ccgccagata
    15121 agcgaagtag agccactgca ccgcttcctg cgcattctgc gccgggcgag agatatcaaa
    15181 gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct ctgccagctc
    15241 ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag actggagatc
    15301 ggcaaattgc agttcgcgtt cacgtaccag ataactgatg ccatacagcg ctacgcggcg
    15361 atagtcaccg ataatgcgcc cacggccata gccatctggt aaaccggtca gcacgccaga
    15421 tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt tatgggtttt
    15481 acgcagatcg gtaaacagat attcaaattc actgtccatt tcacggccat aggcgtggaa
    15541 tgaactttta atcatattaa tgccaccgaa cgggtgtagc gcacgtttca acggcgcatc
    15601 cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat catgagcggt
    15661 aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt tttcgatacg
    15721 gataccttcc attacttttt cccacaattc cgtggtggca ggcgtcgctt cggcgaggaa
    15781 agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga cattaatttc
    15841 gtttttccag tccgtacctt taaagccaag ccatgcgtcg gcgtacagct tatcactggt
    15901 atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa attatgcaaa
    15961 ttctgcgggc gcgttaactt tgcctaaatg aatggcatcc aaagcaatca ttttttcttc
    16021 gttagtcgga ataacagcgc aaatgacacg cgcattttca ctggaaacaa ttcgctcacc
    16081 acaggagtta gagcgattat tcatttctgt atcaatctct acgcctaata cagccaaatg
    16141 ttccatgacc agacgacgaa ttaagcttga attctctcct attccgccgg tgaatataat
    16201 tccatccagg cgacgtaatg aagctgcgtg tccggcaata tgacgggcaa ttcggtgaac
    16261 aaaggtttta attgccagtt gcgcgcgttc gtgaccttca tgccaggctt tttccagaac
    16321 acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat tcactacgcg
    16381 ttccaggtca cccaggctct ggttggtttg gctggcgacc caggacatcg caccaaaatc
    16441 gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc ccattgaggt
    16501 gtcaacactc tgaccgttgc gaaccgcgca gattgacgcg ccattgccaa gatgcgccac
    16561 aaccaggccg gagtcatctt ccgccagatt cagcagcgaa tgtgcgcgct gggaaacata
    16621 gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat aatatttcca
    16681 cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg tatcaaatac
    16741 cgccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac ttaaattggc
    16801 gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga cttcatcggt
    16861 aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc caattaaggc
    16921 cacactgtca tttaaattcc gtttttccag ttcaaatgca attgccttca atgcaccttc
    16981 gtagctgtgg tgagccagcg gtgctggctc tcccccattt acggataaga atgcattttc
    17041 cgagttaata ccgtcggcaa cacctgccat taatacttca cagtcgctgg catcgagcac
    17101 ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa attcattcat
    17161 ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc aggatggtca
    17221 gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc gccagagacg
    17281 gcgctttacg gatggcatac atcggcaaca ggcacagcag ggatgcgata atcggtgcgc
    17341 ccatggcttc aatcaggtca aggatgttcg ggttggcgta ggcaacaacc caggtggagc
    17401 ccatgatgaa gatcatgctg atagtgttca gtttacccag cgacacttta gttttgtcgc
    17461 ctttataacc aaacttcagg accaggccat tcagaccttc cagcgttccc agatagtgac
    17521 cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat tccagtgtaa
    17581 tcgcgaacgt tgttttggta ccggtcatgg acgcaaagtg gttagccaga taagaaagca
    17641 ctggaatatt ctgcgctttg gcttccgcca tgttggccgg agacagcgta aacaggcagc
    17701 taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa atgatttggg
    17761 aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca cgtttagaaa
    17821 ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg gaaatcccca
    17881 gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg aggtcaacct
    17941 ggtcgataac tgcagagttc cagtaaggga tcagcgacag agaaatcagc accaggctgg
    18001 cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta ccaaaccaga
    18061 tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc agcggtgcaa
    18121 atccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg ccataaatcc
    18181 acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg ccagttttac
    18241 caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca gaaagacaca
    18301 gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc aacatcagaa
    18361 gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc ccggcgccga
    18421 ttgccgtacc aaacaagcct aaagtccatg tggtatctga tttacgccag gacgattgtt
    18481 ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa cgaattaatt
    18541 aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag atcgatattg ccgccggaaa
    18601 taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt aataatgcag
    18661 cacatgccag agcgcctgcg ccttcggtga cgactttatt gcgctgaatt aaggcaatca
    18721 tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat tcacgaacga
    18781 tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg gtgccggtag
    18841 ttcggtgcgt ggttatttct ccggagtgga aagaagcagc catgccgtga acgttttcag
    18901 actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc gcaataccag
    18961 caattaaacc gccaccacca attggcacaa tcacgttatc gacatcatag agatcttcca
    19021 taatttccag accaatcgtt ccctggccag caatcacttt cggatcatcg taaggtggga
    19081 taaaaatacg gccttccatt tcgacaattt cgctcacttt agcgatagtg tcgttgaagt
    19141 tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact ttggattttg
    19201 gcgcaccttt tggcatcacc actttaccgt cgatacccag catcgcgcag gagagcgaaa
    19261 ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt tccgcatcgg
    19321 tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta cgctgcatat
    19381 tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg gagcgaggca
    19441 tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaataata tcatcaatag
    19501 caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat taaatatgta
    19561 ggtaaccgta accgacacct gcaaaacaga caggtggatt atttatgttt aaataaaaac
    19621 aaataactaa ccaacttcta ttaattgcct tcgtctacac ccattataag atgaatactc
    19681 tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt tcgaccatac
    19741 cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga atagtaataa attgatttga
    19801 accaaaaggt gacgtcatat cacaaggaat tacagttaag aaatcagcat tgagaacaag
    19861 attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga tgccatttct
    19921 ttgtaacgta gtaagaagct cgctgtagta ccccatattg gtttgtggca acacccactg
    19981 ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact tactggctac
    20041 cagcacaaac tcggactcga acagcgactc aacatgtaaa tcctgaagct tcatttctgc
    20101 acttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta agaacgaaga
    20161 cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga acttgttgat
    20221 catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga cttccaccac
    20281 cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac gggtaatgga
    20341 ttcggaacgg gagagtaaca attgaccggc aggtgttaat gttacaccgg tatttttccg
    20401 caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac tgacggccgg
    20461 ttgagttaac cctaattctt ttgcagccga gccgatagag ccacttctaa tgacttcctg
    20521 aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga cctacgttaa
    20581 ttacctcatt gacggcatga agtgtatcaa aatgaaatga acaggatatg tgcgaccact
    20641 cacaaattaa c
//
LOCUS       DAJSKJ010000060        20152 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000060 DAJSKJ010000000
VERSION     DAJSKJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.060"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            63..1118
                     /gene="phoE"
                     /locus_tag="ON345_001896"
     CDS             63..1118
                     /gene="phoE"
                     /locus_tag="ON345_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR3071963.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1157..1558)
                     /gene="crl"
                     /locus_tag="ON345_001897"
     CDS             complement(1157..1558)
                     /gene="crl"
                     /locus_tag="ON345_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR3071964.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1616..2860)
                     /gene="frsA"
                     /locus_tag="ON345_001898"
     CDS             complement(1616..2860)
                     /gene="frsA"
                     /locus_tag="ON345_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR3071965.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMLSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2952..3410)
                     /gene="gpt"
                     /locus_tag="ON345_001899"
     CDS             complement(2952..3410)
                     /gene="gpt"
                     /locus_tag="ON345_001899"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3071966.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3671..5128
                     /gene="pepD"
                     /locus_tag="ON345_001900"
     CDS             3671..5128
                     /gene="pepD"
                     /locus_tag="ON345_001900"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR3071967.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5185..5799)
                     /locus_tag="ON345_001901"
     CDS             complement(5185..5799)
                     /locus_tag="ON345_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR3071968.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5796..6935)
                     /locus_tag="ON345_001902"
     CDS             complement(5796..6935)
                     /locus_tag="ON345_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR3071969.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQHVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7181..7633)
                     /locus_tag="ON345_001903"
     CDS             complement(7181..7633)
                     /locus_tag="ON345_001903"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3071970.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7630..8685)
                     /gene="dinB"
                     /locus_tag="ON345_001904"
     CDS             complement(7630..8685)
                     /gene="dinB"
                     /locus_tag="ON345_001904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3071971.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8756..9679)
                     /gene="lafU"
                     /locus_tag="ON345_001905"
     CDS             complement(8756..9679)
                     /gene="lafU"
                     /locus_tag="ON345_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR3071972.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9793..10791)
                     /gene="flgK"
                     /locus_tag="ON345_001906"
                     /pseudo
     CDS             complement(9793..10791)
                     /gene="flgK"
                     /locus_tag="ON345_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(10980..11279)
                     /locus_tag="ON345_001907"
     CDS             complement(10980..11279)
                     /locus_tag="ON345_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HCR3071973.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(11279..12379)
                     /locus_tag="ON345_001908"
     CDS             complement(11279..12379)
                     /locus_tag="ON345_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR3071974.1"
                     /translation="MQNWIKTVVVAVSLALPGVVLAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINSSFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(12394..13059)
                     /gene="flgH"
                     /locus_tag="ON345_001909"
     CDS             complement(12394..13059)
                     /gene="flgH"
                     /locus_tag="ON345_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR3071975.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKFSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(13263..14048)
                     /gene="flgG"
                     /locus_tag="ON345_001910"
     CDS             complement(13263..14048)
                     /gene="flgG"
                     /locus_tag="ON345_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR3071976.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAANDMLQYISQTL"
     gene            complement(14075..14812)
                     /locus_tag="ON345_001911"
     CDS             complement(14075..14812)
                     /locus_tag="ON345_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HCR3071977.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(14812..16014)
                     /gene="flgE"
                     /locus_tag="ON345_001912"
     CDS             complement(14812..16014)
                     /gene="flgE"
                     /locus_tag="ON345_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HCR3071978.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     DAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMFQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(16099..16812)
                     /locus_tag="ON345_001913"
     CDS             complement(16099..16812)
                     /locus_tag="ON345_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HCR3071979.1"
                     /translation="MNTLALNAQSPQPTATQESSNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPQGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(16812..17243)
                     /gene="flgC"
                     /locus_tag="ON345_001914"
     CDS             complement(16812..17243)
                     /gene="flgC"
                     /locus_tag="ON345_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR3071980.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(17246..17581)
                     /gene="flgB"
                     /locus_tag="ON345_001915"
     CDS             complement(17246..17581)
                     /gene="flgB"
                     /locus_tag="ON345_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314272.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR3071981.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            17663..18400
                     /gene="flgA"
                     /locus_tag="ON345_001916"
     CDS             17663..18400
                     /gene="flgA"
                     /locus_tag="ON345_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR3071982.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPEVMTKSLVARDTPLTANDVELKPYNVSAQRRDVLMVLDDAI
                     GFSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            18481..18759
                     /gene="flgM"
                     /locus_tag="ON345_001917"
     CDS             18481..18759
                     /gene="flgM"
                     /locus_tag="ON345_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HCR3071983.1"
                     /translation="MKITPTMPGNRTTATTAGSNQPRKTTATTTTTLSADNITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            18772..19197
                     /locus_tag="ON345_001918"
                     /pseudo
     CDS             18772..19197
                     /locus_tag="ON345_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
     gene            19198..19860
                     /locus_tag="ON345_001919"
                     /pseudo
     CDS             19198..19860
                     /locus_tag="ON345_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            19965..>20152
                     /locus_tag="ON345_001920"
     CDS             19965..>20152
                     /locus_tag="ON345_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HicA"
                     /protein_id="HCR3071984.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFST"
BASE COUNT         4516 a         5519 c         5317 g         4800 t
ORIGIN      
        1 tcctgttttc aacggggctc tcccgctgaa tattcgcgcg ttaattaaaa tcaggaatga
       61 aaatgaaaaa gagcactctg gcattagtgg tgatgggcat tgtggcatct gcatccgtac
      121 aggccgcaga aatatataac aaagacggta ataaactgga tgtctatggc aaagttaaag
      181 ccatgcatta tatgagtgat aacgacagta aagatggcga ccagagttat atccgttttg
      241 gttttaaagg cgaaacacaa attaacgatc aactgactgg ctatggccgt tgggaagcgg
      301 agtttgccgg aaataaagcg gagagtgata ctgcacagca aaaaacgcgt ctcgcttttg
      361 ccggattgaa gtataaagat ttgggttctt tcgactatgg ccgtaacctg ggcgcgttgt
      421 atgacgtgga agcctggacc gatatgttcc cggaatttgg tggcgactcc tcggcgcaga
      481 ccgacaactt tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac accgacttct
      541 tcggcgttat cgatggcctg aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg
      601 acgttaaaaa gcaaaacggc gatggcttcg gcacgtcatt gacatatgac tttggcggca
      661 gcgatttcgc cattagtggg gcctatacca actcagatcg caccaacgag cagaacctgc
      721 aaagccgtgg cacaggcaag cgtgcagaag catgggctac aggtctgaaa tacgatgcca
      781 ataatattta tctggcaact ttttattctg aaacacgcaa aatgacgcca ataactggcg
      841 gctttgccaa taagacacag aactttgaag cggtcgctca ataccagttt gactttggtc
      901 tgcgtccatc gctgggttat gtcttatcga aagggaaaga tattgaaggt atcggtgatg
      961 aagatctggt caattatatc gatgtcgggg ctacatatta tttcaacaaa aatatgtcag
     1021 cgtttgttga ttataaaatc aaccaactgg atagcgataa caaattgaat attaataatg
     1081 atgatattgt cgcggttggc atgacctatc agttttaatg attattgccg gatgtgatgc
     1141 atccggcaca tttcactcac gccgttaact tcaccggctc gtcacgaaaa tcatccgccg
     1201 gttccagctt cagattcagc gtcgtcagca gttcacgcag cttctcgtga aactcacgca
     1261 gggtgtgctc cagtcgttca accacttcag tgtcttttac cggaacgctc ttccagtcgc
     1321 ctgctttatc gaacagacca aactggtagc tgtaggtaaa acgggattcc tgcgcttcca
     1381 gctccatcca ccagccccag aattcacgca cttccggtgc cggtttcacg ttgacgcata
     1441 cagccagaca atcgaaaaag aatcgattat cttcgcactt accttcacga atatacgggc
     1501 ctagtgcggt aaattttttg atcaatctgc tcttcgggtg tccactcggt aacgtcattg
     1561 ctatctcctg ttgtgatgca actgttttac caaattggca aattttagca aatttttaac
     1621 acaagcgttt ttcgatccag ccggtgattt cctgaagacc tttgtcaaaa ttccgataca
     1681 ccgggttaaa tgggatctct aataatttac cgtcagcaga tgatgaggtg attaagcgtg
     1741 agtcctcttc cgggctgaac ggatcgttct tccagtagcc tgataacatt ggcgttgggc
     1801 agcgacgtcc cagcaatcct tgcactttta atgaatagcg attcagctcc acgcgcaacg
     1861 cgtcatcgga agcatcatgc atccccaaac gactcgccag aacgtcaaga tacatttccg
     1921 gcacctgttg ctggcactta aaatcactca acagggtatg aactaccgga ccaagacagg
     1981 caaccgcttt cagacgcggc gattcaaggt atgccagacg cacggcaacg ttagcgccga
     2041 aacggaaacc aaaggccgcg acgcgagtgt gatccaccca cggtacgtta ggcagcgcct
     2101 ttaagacgtg ctgatgcaac aggctggagt cctgggtgag cttccatttt gaagaaaagc
     2161 ccaccgacag catatcaata gtcagcatcg caatgccgcg cggcgcaaaa taacgttcat
     2221 acaggctgta atagtccgtc tgcatcgcat ccagaccacc acacattaat actgtcggga
     2281 acgggccatc gcctttcggc atatgcaaaa agccggtgat gggcgcaccg ccgggtacgg
     2341 taaactccat ctgccgcatc gtgcccggca gacgctgggc ggcctcttca taggcgcggt
     2401 ttgataaagc ctgcgcttgc tcggccaggt catctccttt cagatgagga taggcggcaa
     2461 tgttgtacaa cgtagccgca tgcagccagt gtcgaccact gagttgcgga tcttcttccg
     2521 cacaggcttt ttgttgccac accatcgcct gggtggccca ctcataaatc cagttgccac
     2581 cacggtagcc aatcacggta tcgtataaat catcatcggt acgttcagca tcgctcatca
     2641 caatacgtgc ctggacgtcg aggatttcac gtgggtcaat gccgcgccag atccacatca
     2701 gacggttaat catgcgatac cagtgaggga tggttttacc atcaagagcc gactgcacag
     2761 gcggttgtgc gccgtgatta aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc
     2821 ggggtttgaa cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac
     2881 ttactggaaa ctattgtaac ccgcctgaag ttaaaaagaa caacgcccgg cagtgccagg
     2941 cgttgaaaag attagcgacc ggagattggc gggacgaata cgacgcccat atcccacggc
     3001 tgttcgatcc aggtatcttg cgggatatca acaacataat catcaaccag cggacgacca
     3061 gccggttttg caaagatggt gacaaagtgc gcttttggat acatttcacg aatcgcaacc
     3121 gcagtaccac cggtatccac caggtcatca ataacgatga agccttcgcc atcgccttct
     3181 gcgcgtttca gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg
     3241 gtatcgacat gacgaatacc cagttcacgc gccagtaacg cacccggtac cagaccgcca
     3301 cggcttacgg caataatgcc tttccattgt tcagaaggca tcagtcggct tgcgagttta
     3361 cgtgcatgga tctgcaacat gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc
     3421 ccagcctgtt tatctacggc ttaaaaagtg ttcgagggga aaataggttg cgcgagatta
     3481 tagagatctg gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg
     3541 cctctccctg acgcgggata aagtggtatt ctcaaacata tctcgcaagc ctgtcttgtg
     3601 ttgacaacat tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag
     3661 gagacttaac gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc
     3721 caaaatctgt tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt
     3781 tggttgggca aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat
     3841 tcgtaaacct gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct
     3901 cgatatggtg ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca
     3961 gccttatatt gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg
     4021 cattggtatg gcctctgcac tggcagttct ggctgacgaa aacgtggttc acggcccgct
     4081 ggaagtgctg ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag
     4141 caactggttg caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta
     4201 catgggttgt gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt
     4261 tccagctggt tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg
     4321 cggtgaaatc cacgttggcc tgggtaatgc caacaaacta ctggtgcgct tcctggcggg
     4381 tcatgcggaa gaactggatc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc
     4441 catcccgcgt gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgtcctgaa
     4501 atctctggtg aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa
     4561 tctggccttg ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg
     4621 cgataccttt attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt
     4681 ggcaaaaggt gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa
     4741 cgtagaaatt cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag
     4801 catgctggat tcgctgggta aactagctgg cgcgaaaacc gaagcgaaag gcgcatatcc
     4861 tggctggcag ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg
     4921 tctgttcaac aaaacaccga acatccagat tatccacgcg ggcctggaat gtggtctgtt
     4981 caaaaaaccg tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca
     5041 ctctccggat gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga
     5101 actgctgaaa gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc
     5161 gccttccggc agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaatgtccg
     5221 tctcgggttg ccacgttcaa tctggtgatg gaacattcgc cgctgcgatt tcagcaccgc
     5281 gctattttcc tgttgctgct gctccagctt ccaggcaatc agcaatcgtg ccagccgctt
     5341 gttagcatgc tgactacgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt
     5401 ggcgcgtacc gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcaa
     5461 cgtctcataa cggattgcat ccgattgttc ctgctcatca gcggtaaaac gcccaatgcc
     5521 cagaaaccag tttttgcgcc catgatgagg ccgatacgga ctcggacaaa tccactgaat
     5581 agtgccgcac cagctttcgc ttaatgccca tgcgttatcg ccatccagag aaaccagagc
     5641 tgaacgcagc gtgtcagagt agcggcccgt ttctgtttcc aacaccgtta ccgcgacgtc
     5701 ttgtcgggca gcttctttaa ttaatcgctc cagcgccttt ttcactgcga gacaacattc
     5761 ttccggcccc tgcgctgagg agagttgtag caagatcatc cttttttccc tccactgttt
     5821 ttgagcgtta gcaccggacg cagccgcgcg acaggaataa ttaaaccggc ctgcaccaga
     5881 cattgcacca cgctttcagc cgatttatag gcttgtggcg cttcttcaaa gatgagttgt
     5941 ttatcgcgac aaattacccg actgccaagt tcagtccgcg agagttgcgt caccgtgtat
     6001 ttcgctgcca gacgcccttt acactcggtg cgcccccatt tacgccctgc cccatgcgcc
     6061 agcgagtgga gcgttttttc gttcgcaaca tgctgtacaa tccaggagta atcaccacgt
     6121 gaaccgggaa taatcaccag accgttgtca tccggcgtgg cacctttacg atgcaaccag
     6181 ccctgttgat cgccgatttg acacgcgcta acaaagttat gcgccacatc cagaaccgga
     6241 cttcccgtgg ccttaacttg ttgcattatg cgcaaagcga tcatctggcg attaatacac
     6301 gcaaacgcca gcgcatcgtc atgttccgca atataccgta gcgcgtcgtc actgccttga
     6361 ggcaaaccat gatgcgagaa cgaggcaata tgccgctgta aaatagactg ccccaccccc
     6421 cgcgagccgc tatgaaccaa cagttgcaga tgttgtgcat ccagacctgc cagtgcaaac
     6481 agttcagcgt tgatgatttg atcaacttgt tgcagttcag cgaaatgatt gccgccaccg
     6541 atagagccaa gcgagctgcg ccacggatgc tgtgcaaatg ctgacggcag gttttcctcc
     6601 agccagcttt cttcagcaac gtcatccaga tctgataatc gcttttcaaa cttatcggcg
     6661 ttgtatttgc gagcgagaat atctgtttgc catagcgcca taccgcagcc gatatcattg
     6721 ccgaccagtg ccggataaaa acgaccaaca gagaagaacg ctgcgccaat cggatagccg
     6781 cgtccgggat gtaaatctgg catccccact acgcgctgca tgttgggtaa atttgccgtg
     6841 gtgtgtaatt gttggatcgc taaactctcg atccacaggt catcagatgc gatggtaaat
     6901 accgcatcgg ataagggacg aatataattg cccatatacc attccgtaaa tagactcgtc
     6961 atacttcaag ttgcatgtgt tgcgacttga attattgaga gtaacaatta aaaaatcagg
     7021 aaatggcaga cgaatccagg gcaagaaaaa aagaattaat cctggaaagg tctgcaaaga
     7081 gtcattgata aaggatttgt catgatttac ctccttgcag tttgtggaat aagaaaacgc
     7141 gcacatacta ggcttgcgtt attttcattg caagctggat ttaatgttgc tgtttataac
     7201 gcatataaaa attaataaac cactctcccc gacattcaac gcgttgctgc ttaactgtct
     7261 gaaaaccata acgttcaaaa aagggttttg cggttatgct tgcgtccacc gtaagttcgg
     7321 attcagactt aatcaaaggt tttaacaaag cgctggcaac cccacggcgg gtgtattcag
     7381 ggtcaacaaa taacatatcg atatagtgtt caacgcaggt aataaagccg accagttttg
     7441 cattaatgac tgcaacccgc acttgtgatt tcgcgagttt ctccttccag cgagattcgt
     7501 caatctgcgc ccaggcggca atttgttgtg gtgaataatg ctgactggca gtcatcgtaa
     7561 ccgctctaat gaaaatagcg catagttgct gaaaatcgcc aggctgatag tctcttattt
     7621 gtatgttatt cataatccca gcaccagttg tctttccatt tgcgggtcaa gcaacgtcac
     7681 atgcagcccc accagtcgca caccgcgccc gccgcggcgt tcatcccagg ttttacgcgc
     7741 ggtggcgatt aagtcagctt tattcagccg cggccagacg tgctcctgag tggtttgctg
     7801 aaaatcatca aactttaatt tcactccctg acgggcgatc agtaaatcag gttttacctt
     7861 tgccagacgg cgttcaagtt ccggatacag ccgctcgata atcgcttcac attcagacca
     7921 gtgatgaata tcttccgcca tcgtgcgttc cacgccgacg gattttcgta accgttcgct
     7981 gttaacgtcg cgctcgtcaa tcccctgact acgctcccac aaaatgcggc caaatttgcc
     8041 aaagcgttta agcagaatca ccagatcaca cttttgtaca tcaccgcagg tccgcagccc
     8101 catcgcttcc agttttgccg ccgagacttt gccgacgccg gggatttttg ccagcggtaa
     8161 ggtttgtaaa aatgccggaa cttctgccgg cgtaatcaca aactgaccgt tgggtttatt
     8221 catgtcggag gcgattttgg cgagaaactt tactggtgcc acgcccgcag acgctgtcag
     8281 ttgcagctcg ctgaaaatcg tctggcggat ttcctgggcg atgagggtcg cagaaccgtg
     8341 gcaatggacg ctatcggtga cgtcgagata agcctcatcc agtgacaacg gttcaatacg
     8401 cgaggtgtag cgcgagaaga tttcacggat atgatttgag gcttctttgt aggcgtcaaa
     8461 gcgccccgga agcaaggtga gatgcgggca taatttgagc gccatccctg tcggcatagc
     8521 gctacgtacg ccaaatttac gcgcgggata attggcggtg ctgatcaccc cccgacgttc
     8581 acggctgccg ccaatagcaa tagggatatc gcgcagggcg ggattgtcgc gcatctccac
     8641 cgctgcgaaa aagcagtcca tatccacatg aatgatttta cgcattactc acctctcaac
     8701 actggtaaag tatacagtga tttcagggtt tgagaaatgc gtaaagattc agcatttaac
     8761 gcgttcgctg cgaaagcacc tgctgcttat ccagcttttg caccagcggc tgcaccactt
     8821 tatccccatg ctgaccaaag tattgataca gcgtatcgga cgcacttttg gtcagcacca
     8881 taatctcaat gcgccggttg cccgcgcttt gcggattttt cgcgtccagc agcatctggt
     8941 ccgccattgc gcttacctgc atcactttat cttccggcat tccggcctct tccagcaccc
     9001 gacgagccga aagcgcgcga tcgcccgaaa ggttccagtt gttgtagata ttgtttttgt
     9061 aggccatcgc atcggtatgc ccggtaataa taattttgtt atcgagcgag tcgaacactg
     9121 gcgcgagctc caccagcaaa gttttaaaga acggcataat ctgcgcgctg ccgcgttcga
     9181 acatattgcg gttctggtcg tctttaatca gcacgcgtaa tccctgggga acaatctcca
     9241 tctccagatt cgcttccata tgcgcatcac gggcaatggt gttgatactg gttgccagct
     9301 cgccaagctc ggtggcggat ttcttcttca gcagcgccgt tttctcattc acatcacgcg
     9361 cttttttctc cgtctcttcc ggcaccgcca cggtggcagg ttttggcgga tgagacgggc
     9421 tgattttatt taacggcgac agcccaccac cgttaaaaat cgactgcccg tgcagagcgg
     9481 cgataatact ttcacgctcg gatttactga cgctgttgac aatccacagc gtcataaaca
     9541 gcgccatcat tgccaaagta aagtcggcga acgctacctt ccacgccccg ccgtggtgcc
     9601 cggcatgatt ctttttgatc tgccgccgga cgatggtggt ttttgcccca cgctcgcggc
     9661 ggttgcccgt ctttctcatg tttattcttg ctccagcatc gcattcaccc acgcgtcgag
     9721 gttggcgaag gtcggtttag atgccagatg cagcagctta cgcccggcat ccaccgccag
     9781 cagcgtcggt ttaacggtca gcgggccatc ggcattgctg gcgtcataaa taaacagcgg
     9841 ctcgccgggg ttaccgttga gatcgtagcc ctgcgccagc tggttgttga ccgcatcggc
     9901 gaactgcgac gccatgctgt tgatggtgtc ggtcagcggc gtcagcacat cgttttgata
     9961 atcaaacagt gcgcctaatg aaccgccggt gtcggtggtc atcgtcgagg tggtgccagc
    10021 gaacgtcagc gacatggttg gcgtgccatc ggcgttggtt tccagcgcga tggtcgagct
    10081 ttgctgccca ctcaccagcg gctgaccgtt tttcagggta acgttgtagt tgccctggtc
    10141 gtcgatattg acctgcacat ccatcatccc gctcagttct tccaccatct ggtcgcgggc
    10201 gtcgtacagc gcggaggcgt tatcgccatt ggcttcagcc tgagcgattt gctggttata
    10261 gctggcgatg ccgctggtga gcgtattgat ttgcgacacc atcgcctgct gctggctgat
    10321 gatttccgtg ctttgcgagt cgatgtaatc cagcgtgtta tcaatacgca acgacagcgc
    10381 cccggcttca ctgatcacct gctcgcgcag ggcggaatca tcggggctgg tggttgcttc
    10441 gttaagggcg gcgaagaagt tatcgaagcc gccgctcagg ctgctgttat cgtcgctcag
    10501 taccgcttcc agttggctga gatacccctg ctgggtgctg taatagccgt aatcgctggc
    10561 ggcataccac acctgattca cctgatactg gttggagacg cggcgaatgc tgtcgacctg
    10621 tacgccgtta ccggcgctgt tcggcgaacc gccgctggca ccgatggtgc tgatctccgc
    10681 cacctgacgg gtgtagcccg tggtcatggc gttggcggtg ttttgcgccg tgacgttaag
    10741 ctccacctgc gcggttgatg caccgctgta gccgatgtta atcatgtcca tgttggatcc
    10801 ttaccggtaa atcgtgccgg ttaaaagcgg cgtaaagttg gggattctta agttccgtaa
    10861 accgggtgcc gaacgccgga tgcggcgtgg acgccttatc cggcctacat acgggcgact
    10921 cgtaggcctg ataagacgcg acagcgtcgc atcaggcatc gtgcaccgaa cgccgaagct
    10981 catttatgct tcggcgacaa ctgcttcaca atcatcgccg caatgccaat cccgtgctgc
    11041 tgcgccaggg tgttacacaa ctcatcgtca taaaacccct gcatcatgcg caccgaatcg
    11101 ctgttaaacg ggttatcttt tgaatcaaac aactcgttgg ttttccgcat ctctttcagc
    11161 atgttgcgta aaaaaatcgc ctcaaactgt tcggcggcct gcttgatatc attggcctga
    11221 accttcggcc ccattaacgc gtcgctgccg tcaatcccgc cgctggcgtt cactttcatc
    11281 agatcacctc caggtcggca tccagcgcgc ccgcctcatg cagcgcctgc aaaatcgaca
    11341 taatgtcgtc cggcgtcgcg cctaaactgt tcagcgcatt gacgagcgtt ttcaggctgc
    11401 tcgactccgg caaactcacc acgcccggac gggcatgatt caccgcgata ttgctctgcg
    11461 gcgtaaccgc cgtgcgccca cccgcaaagg cattcggctg gctgacgttg ctggtttcgt
    11521 taatcgaaac ggtcaaacta ccgtgcgaca ccgccgccgc gtgcagcgca acgccatcgc
    11581 ccatcaccac cgtgccggta cgcgagttaa acaccacgcg agcgcggatt ttctccgcct
    11641 gtacctgcac atcgtccagt tgcgacataa acgccacccg cgcgccgggg ctggtgggcg
    11701 cgcgcacggt aacgttggtg gagctctgcg cggtggcaat gccgccaaac gaggagttga
    11761 tcgccgctgc aatgttgttg gcgtctttaa acgacgggcg tttcaggttg agggtgatgg
    11821 tgtcgcccat ctgaaaatca ctggggatct cgcgctccac cgttgcgccg ttaggaatta
    11881 acccggcggt cggtgtatta accgttacgc tcgagccgct ggccccggtg gcgttcatcc
    11941 cgccgaccac cacgctgccc tgtgccaggg catacacttc accatctgcg ccgtgcaact
    12001 gcgtcagcag caaggtgccg ccacgaatac ttttcgcatc gccaatcgac gagacggtga
    12061 catcaatggt ctgcccgcgc gagtacatcg gcggcaacgt ggcgctcacc gccactgcgg
    12121 cgacgttttt caccttcggg tcgattttat tcggcaattg cacgccgaac tggcgcagca
    12181 tattggtgat ggtctggttg gtgaatttga cctggttttt atcaccggta ccgtcgaggc
    12241 cgaccaccag gctgtagccg accagttggt tttcacgcac gccctgtacg ttgaccagtg
    12301 actccagcga ttgcgccagc accacgccgg gtagcgccag gcttacggct acgaccactg
    12361 tttttatcca gttttgcatt gcactctccg gcattaaatc gggaacagag gatggttaaa
    12421 caaccgcgtc agccagcctg cggcattggc gtcgcttaac gcgccacgtc cggcatagga
    12481 gatacgggcg tcggcgatcc gctgggagga aaccgagttg tcgttctgga tgtcgtcggc
    12541 gcgcaccagg ccgcttaagc ggatgtactc atcgccctgg ttgagggtga gccatttctc
    12601 gccgcgaatt tccaggatgc cgttgggctg caccgcatgg acggaaacgg tgatctcccc
    12661 gcgcaggctg ttctgctgct gggaagtggc gctgccgtca aagtcgcggt tggcgtcgat
    12721 agagccggaa aacttatctt tagtgtgacc aaaaatggtc ggtgcgccga tatcgacggt
    12781 gttgcttttg ccgaagttgg ttttcgcctg tttgctcgac tgggtggatt cttcgaggat
    12841 caccgtcagg atatcgccca cgcgataagc ccgccgatcg gcggtcagcg accagttgta
    12901 gccagtttca aatacaccgc cagcacgccc gtctgccgga ggcggtgctt cggttttcgg
    12961 tggcgcaaag tatgcgtcgt cttttttgac cagaatggcc tgcgattcac acccgctaag
    13021 caggggaagc agcgccacca gccagagata atttttcacg ttctgcctgt ttaaatacag
    13081 tgagttatgc gcttttgtag gtcggataag acgcgtcagc gtcgcatccg acaacaagcg
    13141 tgtgcacatt gcccgatgcg cgtgtggacg gtttactaac ggttaatcaa ccaaactacg
    13201 tgcaccgtcc actcgcttat cgtgcctaca agggcgtcag cgtcgcatcc gacatttcgg
    13261 ccttacagcg tctgactgat gtactgcaac atatcgttcg cagccgacac cattttggcg
    13321 ttcatctcgt aagcccgttg cactgtaatc atcgccacca tttcgttgac gatatctacg
    13381 ttcgagcctt cgagggcatt gtcctgcaaa gtgccaagcc catcttcgcc cggcacacct
    13441 tcggtcggct gaccgctggc ggcggtttcc agatacaggt tgtcaccctc agcggaaagc
    13501 ccagccgggt tagcgaagtt aaccagcgtc agttgcccta actcggtcac gtcgctgtcg
    13561 cctggtagga tcgccgtcac cgtgccatct tcgccaaacg cgacgttggt cgcccccgcc
    13621 ggaacgtcga tttccggctg caacggcaag ccctgtgagt tggtcagtac gccgtcggcg
    13681 ttcacctgta agttgccatc gcgggtatag gcgatatcgc cgttggcctt ttgcacctgc
    13741 aaaaatccct gccccataat ggcgacattc atcgcgttgt cggtggtttc gacgttgcct
    13801 tcggtgaagg ttttctgggt accgacaatg cgtacaccgc tcccgaattg cagcccggtc
    13861 ggcataatgt tgttctggtc gagcatcgcg cccggcgctt cctgggtctg gtagaacaga
    13921 tcctgaaaca tcacccggtc acgcttgaag ccggtggtgt taacgttggc gatgttgttg
    13981 gcaatcgcgc tcatttcagc atcctgcgcc gagaggccgg ttttgctgat ccataatgct
    14041 gcgttcatgg tgatttattc cctggtgtct gttatcacga gccgcgcaat aagcggttgc
    14101 ccgcgtcgct gatatcttcg gcggttttca tcatcttgat ctgtgcttcg aactggcggt
    14161 tcatggcgat ggacgacatc atttcgctta ccgccgagac gttgctgccc tcgagaaaac
    14221 cgccgctgac tttgatgttt tcatcgcgct gggccggaac gccgtcggca gtcaccagca
    14281 tcccttcagc atttttctcc aggttagcga ccgggatatc caccagcttc aggcgatcga
    14341 tatccatcgt cgcggtcaca tcgccgtcat cgggcgtcac cgacagcgtg ccgtcgctgc
    14401 caaacgaagc aatggcgttc ggcggcagaa tgatcggccc gttatccccc agcaccagat
    14461 tgccgtcgat ggtcagttgc ccctggtcgt cctgctgaat gttgccgttg cgggtataga
    14521 cttcgttacc gtttttatcc tgcaccgcaa tgtaacccgc accctgaatc gccacgtcca
    14581 gcgggcgttc ggttttctcc gcaacacccg tgctgtcgtt cacgccgctg gccccttcct
    14641 gcgccatata gcgcgtgtcg aagccgccgc ctttcacctt gtcgttggtt gccattgcca
    14701 tatcggcgcg aaaaccattg gtgttgacgt tcgccaggtt gttggcgcta atttgctgct
    14761 catacagggt ctgagaagca ccgctcaggg cggtataaat caggcggtcc attacattgc
    14821 ctggaacagc gcgtcatcga gctgcgtgct ggtggcgata actttggtgt tggcctgata
    14881 gttacgctgg gcggtcatca ggttgaccag ttcgctggtg atatcgacgt tagagctttc
    14941 gagcacgccc gacgacagcg tgccgcaggt gccggagccg ggaacgccaa tcagcggggt
    15001 gccggattcc ccggtttgta cccacgcggt gccgcttacc gcttcaaggc cgttttcatt
    15061 cgggaaagtc gccagcacca cctgcccctg aaacatgcgc tcgccgttgc tgtaggtggc
    15121 gtaaactttg ccatcgtcat caacctgcac accattctga gtcgcggagg cgtagccgtt
    15181 ggcggcgttg gtggtgaccg aaaattcaga gccgtattgc gtacaggtgg agtaatcaac
    15241 ggttaagtcg atgggtgcgg cagcgtcggt ttgaaattca atggtctgcg gcgtggttgg
    15301 cgaggtcagc ttcccggtgt tcgggtcgaa ggttaacgtc gtcgccggaa cgccggtctg
    15361 ctgctgaccg tcgaaggtgt actgcacttc ccaagtgttg tcgctggttt tggtgaagta
    15421 ctggcatacc gaatgttcgt tgcctaatga gtcatacacc gtggtggtgt agctgtcggt
    15481 gtacgagctg ctgttggtgg cgtcgaacgg tacggttgtg cgatcgatag cgtcgtcgct
    15541 ggcatcgaag ttggcggtaa aagtcaggct gctgctggcc tgcgccggga tattgccggt
    15601 tttgatctgg atatcggtga ccgtaccggt ttgcagagtg ccagtgtcat cgaccggata
    15661 gccctgcaaa taagcgcccg aggcgttgac aatatagccg tttttgtcgg tttcaaacga
    15721 tccggcgcgg gtgtaggaaa tgttgcccgc gctgtcgcag gtaacaaaaa agccgtcgtc
    15781 gttaatcgcc agatccagcg cgttgccggt ggagaccagc gaaccgccgc gcgaaatact
    15841 ttgcgccgtg cccgccacgc tgacacccat cgcctggctt ccggcataca tagctgaaaa
    15901 ctgggtagtc atcgacttat agcccaccgt tccggcgttg gcgatgttgt tactgatccc
    15961 gtccagctgt tcgttaacgg cattcagccc cgtcgcggca atttcataac tcattgtgtc
    16021 ttcctgttaa ttcaaatttt gacagcgtgc ccgttgtttt gccggatgcg gcgtgaacgc
    16081 cttatccggc ctacaaaatc aggccacatc actgtcggca tcgggtacgc caaactggct
    16141 aatcatggtg aacggcactt cgccgacgcc tgcaacgttg agcaccggag agctgccgtc
    16201 gagcgggatg cgcacatctt ccacttcgcc ggagacttca atcggcacct cttcttcccc
    16261 ggtgttggtg accaccgaga cgtcgtaatc gcccggcggg ataccgtaat ccgcgggatt
    16321 caccgagaaa tccacctggc ctggcccgac ggacgaactc ccttgcggga tcagcgagac
    16381 ggtgtagtca tcgcctgcgg catcggtaaa gtggagatcg gcggtggtac aggcgtcgtc
    16441 cagcgtagcg cgaccattaa tggtctggtc gctcacctgc aaggcggtgg tctgcaccat
    16501 aatgtcgtcg cccaccaggt taccgagcgc catcacctga atgttgctca tcagcaccgc
    16561 gttggtgttg gcctgcacgc tcatctcttc catcatcgcc acctgcgcca tcgacgacag
    16621 ctggtcgata tattcggtgg cgtcggtcgg gtcggtcggg tcctggtttt ggatctcggc
    16681 gacaaacagc gtcaggaagg tatcgacggg cgagctatcg ctgctgcttt gcgtgtcact
    16741 ggccgccaca ttactgcttt cctgcgtggc cgttggctgc ggcgattgcg cattcagcgc
    16801 gagggtattc atcaggcttc tcccagtttc agcaaacttt gctgcatact tttcacgttg
    16861 ttgagcacat cgacgttggt ttcgaaatcg cgcgaggccg acatcatgtc cgccatctgc
    16921 tccaccacgt tgacgtcggg ataccagacg tcaccgttgg cgtcggcgag cggagaatgc
    16981 ggttcatagc gtttcaccgc cccgccggtt tgcagcacgt cctgtacctg cacgctggca
    17041 ccgtcaatgg catgacgatg tgtacccgcc agcaggctgt ggtgatacac cgcggcaaac
    17101 accgggctgc gcgctttata cgcctgcgcc tcgctgctgg cgggcgcatt ggcattcgcc
    17161 aggttactgg caacggtatt cagacgtagc gtttgcgccg tcatcgccga gccggaaatc
    17221 tgatagatat cagtaaatga catgcttatt tcccttcaat ggcctctctg atgccgctga
    17281 tttgcagatt cagaaaggtc aaactgcttt gataatccat actattttgt gaaaaccgcg
    17341 cctgttcgct gttcagttcc acggtgttgc catcttccga cggctgcatc ggcacgcggt
    17401 actggacatc caccgccgcg ttatttgccc gttgcatctc cctggcaaaa tcaatatctt
    17461 tagccttgaa atttggcgta tcgacgttcg ccagattcgc agtcagtagc tcggtcctct
    17521 caagacgtaa cttcactgcc tgcggatgta ctcccaatgc ctgctgaaaa ctgatcccca
    17581 tttcctgcat cactcctgct tcaatccgca tacgctaagc aatcgccatg ccatttttta
    17641 agtcactgat gagaaagggt ttatgagttt tcaattttgc aggcggaagt tttatttcct
    17701 tctggcgctg gccttaccgg gctacgccgc cgtccatccc gtgcaacaca gcgcgcggga
    17761 acaagtcaac gcccaggtgc tgaatgccgc gagccaaaaa attgaatcgc tggcgcagca
    17821 gcggcagtgg catgactatc gctacacctt taagatctac attccctcgc agattgcgac
    17881 cgccgcgccc tgtactaaaa cacccggcgt cacgctgact tcgcctgcgg aaatcgccct
    17941 caaccggatg aacttcaccg tcagttgccc gcaaagctgg cagatgaacg tggcggtgcg
    18001 cccggatgta ctggtgccgg aggtaatgac gaagtcactg gttgcccgcg atacgccgct
    18061 caccgcgaat gatgtggagc ttaagcccta caacgtcagc gcccagcgcc gcgatgtgtt
    18121 gatggttctc gatgacgcga ttggtttcag cagtaaacac gccctgcaac ccggcaggcc
    18181 aatcaccaaa gaagaactcg tgtctccggt gctggtcggg cgcgatcaac cggtgatgat
    18241 tgtttaccag agcgcgggga tcaccgcctc gatgcccggc gtggcgctga aaaatggccg
    18301 caaaggcgag atggtgaaag tgcgtaacgc cagcagccag cgggtcatca gcgcaatggt
    18361 ggccgaaagc ggcgtggtga cgacggtaag cgcggagtaa tttgcgcagg cgattaaagt
    18421 tttgccgcct gttaaccgct ttaccggagt aacccaccca attaccgaga cgaggttgcg
    18481 atgaaaatca ccccgactat gccaggcaat cgcacgacag ccacgacggc aggctccaac
    18541 cagccgcgca aaaccacggc gaccaccacc acgacgctga gcgccgacaa cattactcag
    18601 gcgggattgc agtccgccca gcaaacgctg aacgaccagc aagagagcga tatcgacagc
    18661 gacaaagtgg cgcaaatgca ggcgatgctg gcgtccggca gcccgcaagt ggacaccgaa
    18721 cagttagcca gcgatatgct gagttttttc cagaactaag aggccgatga agtgagtatc
    18781 cggttacaac aggtgaaggc gttgttgcag ggcattcgtg acgatgacgc gctgtatgat
    18841 tccctgcgcg aacttctcca gcgtcagcgg atctgcatga ttcgccgcgc cagcgaagag
    18901 ttgctggcgg taaacgatga gataacccat cactatgagc agttgcatgg acatagccat
    18961 cagcggcata gcctgctgaa aatgctcggt gttagcgtaa atcgtgacgg tctggcgcag
    19021 gttttcacct ggcttccggc ggtccaaaaa gcggcagcac aacaattgtg gcaacacctt
    19081 gagcaaaaag cggaacgctg caaaacctac aacgacaaaa acggcgagct gttgattcgc
    19141 cagtatgaat ttattcagtc gtttttaggc agcgaagcag attttctgta tcaggaatat
    19201 caggtgatcg acagcgccag cgtgattgct acccatgtaa ataagattgt gcgcagctat
    19261 attcctgatt tatttaacta tgacgatatt acgcagttgc ataaccgttt gtcgtcgatg
    19321 gcaccgcgcc tggcggagga tttaagcgcg gtgctcaatt acagccagtt gctgaaagtg
    19381 taccgggcgc tgctgaccga aggcgtttcc ctgcgcgata tcgtcaccat cgccaccgtg
    19441 ctggtcgcca gtagcgcggt gaccaaagat catattctgc tggcagccga tgtgcgcctg
    19501 gcgctgcggc gcagcattac ccatccgttc gttcgcaagc aggagctgac ggtgtatacc
    19561 ctgaataatg agctggaaaa tctgctgact aacctagtga atcaggcgca acagggcggt
    19621 aaagtgatgc tcgacagcgt gccagtggac ccgaatatgc tcaaccagtt ccagagcacg
    19681 atgccacagg tgaaagagca gatgaaagcg acggggaaag actcggtgct gctggtgcca
    19741 ccgcagctgc gccctttgct ggcgcgttat gcaaggttgt ttgcgccggg gctgcatgtg
    19801 ctgtcgtata acgaagtgcc ggacgagctg gagttgaaga ttatgggggc gttgagttga
    19861 tgggggattc ttttccatgc tttgcagcac ctgacaccat taattgacgt cacactgcaa
    19921 agagactacg atgacaccat gcaatgacgt catggattga tagtatgatt aaggtcagcg
    19981 cgctaggttc tcgtcataga aaaacattcg acgatgtaat gacggagccg ccaaaatctg
    20041 gaattaaatg ggatgatgtt gttgcactca ttaaagctgt gggtggaaca ataaaaaata
    20101 acaacggctc cagacgaaaa tttcagattg gaacgaccaa attcagcaca ca
//
LOCUS       DAJSKJ010000061        20022 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000061 DAJSKJ010000000
VERSION     DAJSKJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.061"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(292..1104)
                     /locus_tag="ON345_001921"
     CDS             complement(292..1104)
                     /locus_tag="ON345_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR3071985.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHVRPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            1366..2127
                     /gene="udp"
                     /locus_tag="ON345_001922"
     CDS             1366..2127
                     /gene="udp"
                     /locus_tag="ON345_001922"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR3071986.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            2268..3695
                     /gene="rmuC"
                     /locus_tag="ON345_001923"
     CDS             2268..3695
                     /gene="rmuC"
                     /locus_tag="ON345_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR3071987.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3790..4545
                     /gene="ubiE"
                     /locus_tag="ON345_001924"
     CDS             3790..4545
                     /gene="ubiE"
                     /locus_tag="ON345_001924"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR3071988.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4559..5164
                     /gene="ubiJ"
                     /locus_tag="ON345_001925"
     CDS             4559..5164
                     /gene="ubiJ"
                     /locus_tag="ON345_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR3071989.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKLLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5161..6801
                     /gene="ubiB"
                     /locus_tag="ON345_001926"
     CDS             5161..6801
                     /gene="ubiB"
                     /locus_tag="ON345_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR3071990.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6880..7149
                     /gene="tatA"
                     /locus_tag="ON345_001927"
     CDS             6880..7149
                     /gene="tatA"
                     /locus_tag="ON345_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR3071991.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7153..7668
                     /gene="tatB"
                     /locus_tag="ON345_001928"
     CDS             7153..7668
                     /gene="tatB"
                     /locus_tag="ON345_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR3071992.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7671..8447
                     /gene="tatC"
                     /locus_tag="ON345_001929"
     CDS             7671..8447
                     /gene="tatC"
                     /locus_tag="ON345_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR3071993.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8489..9271
                     /gene="tatD"
                     /locus_tag="ON345_001930"
     CDS             8489..9271
                     /gene="tatD"
                     /locus_tag="ON345_001930"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR3071994.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQSEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRVAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9268..9756)
                     /gene="rfaH"
                     /locus_tag="ON345_001931"
     CDS             complement(9268..9756)
                     /gene="rfaH"
                     /locus_tag="ON345_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR3071995.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9923..11416
                     /gene="ubiD"
                     /locus_tag="ON345_001932"
     CDS             9923..11416
                     /gene="ubiD"
                     /locus_tag="ON345_001932"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR3071996.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11462..12163
                     /gene="fre"
                     /locus_tag="ON345_001933"
     CDS             11462..12163
                     /gene="fre"
                     /locus_tag="ON345_001933"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR3071997.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12355..13518)
                     /gene="fadA"
                     /locus_tag="ON345_001934"
     CDS             complement(12355..13518)
                     /gene="fadA"
                     /locus_tag="ON345_001934"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR3071998.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13528..15717)
                     /gene="fadB"
                     /locus_tag="ON345_001935"
     CDS             complement(13528..15717)
                     /gene="fadB"
                     /locus_tag="ON345_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR3071999.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15907..17238
                     /gene="pepQ"
                     /locus_tag="ON345_001936"
     CDS             15907..17238
                     /gene="pepQ"
                     /locus_tag="ON345_001936"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR3072000.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     YVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17238..17852
                     /locus_tag="ON345_001937"
     CDS             17238..17852
                     /locus_tag="ON345_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR3072001.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17891..19342
                     /gene="trkH"
                     /locus_tag="ON345_001938"
     CDS             17891..19342
                     /gene="trkH"
                     /locus_tag="ON345_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR3072002.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19354..19899
                     /gene="hemG"
                     /locus_tag="ON345_001939"
     CDS             19354..19899
                     /gene="hemG"
                     /locus_tag="ON345_001939"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR3072003.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         4653 a         5077 c         5464 g         4828 t
ORIGIN      
        1 caaaatgttt cactttaata tctttttatc aggaaggtct gagatgattt ataattaatg
       61 tgaatgtgtt gataataatt aatgtttgtt tgtgaatggg agtagatggt aaatctctaa
      121 ggtgtcttcc attgaagaga ctcatttatg actatgactt gccgactttc ttaaggtcaa
      181 atagcggagt cgtaatctcc aatcaattca tactgattat tttttggggg acagagatgg
      241 cagcaaaacg cactcgttca ctcaccatct caaatataga ctaagaggaa actaactccg
      301 cttcccacca tactgcttaa accattccag catacgctgc cagccatctt tcgcagattc
      361 ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc ccggcgtccg ggtacacgat
      421 aatctctgct ttcgcattag cagcccgcag cgcctggcgc atggtttcaa cgctctcctg
      481 cggaatgctg ttatcctgac caccatataa gccgagaacc ggcgcgttaa gatcggttgc
      541 gatatcaaca ggttgtttcg gtgaattcag cgacttgtcg cccgtcagtt tgccgtacca
      601 cgccactgcg gcttttagct gtggattatg cgcggcatac agccaggtga tacgtccacc
      661 ccagcagaat ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg aagcccaact
      721 ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc acttttgcta ccagaccgct
      781 aagcaacgtg gggatatcgg caaaatcatt cggatcgcct tcgcggaagt aaagttcagg
      841 tgcgatagcc agatacccct ccagcgccag acggcgacaa atgtcgcgga tatgttcatg
      901 cacgccaaaa atttcctgca ctacaatgac cactggcagt gggccatcgc tttgctttgg
      961 tctgacatgg taagcaggca tgttatcccc ttgtgaaggg atagaggtga agcccgccac
     1021 aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga ggcgatgcag caggtgcaaa
     1081 tccagattgt tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat gttgcgcaat
     1141 gttaactata gtcagcatgc aacaaatcac attgcctgaa tcggctcatc ttttatgcag
     1201 tcctgcagaa tgaagagtga tttatgtgat ttgcatcact tttggtgggt aaatttatgc
     1261 aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt cggtaaagtg
     1321 tctttttgct tcttctgact aaaccgattc acagaggagt tgtatatgtc caagtctgat
     1381 gtttttcatc tcggcctcac taaaaacgat ttacaagggg ctacgcttgc catcgtccct
     1441 ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt taagctggca
     1501 tctcaccgcg aattcaccac ctggcgtgca gagctggatg gtaaacctgt tatcgtctgc
     1561 tctaccggta tcggcggccc gtctacctct attgctgttg aagagctggc acagctgggc
     1621 attcgcacct tcctgcgtat cggtacaacg ggcgctattc agccgcatat taatgtgggt
     1681 gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg cgagcctgca cttcgcaccg
     1741 ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg cgctggttga agctgcgaaa
     1801 tccattggcg cgacaactca cgttggcgtg acagcttctt ctgatacctt ctacccaggt
     1861 caggaacgtt acgatactta ctctggtcgc gtagttcgtc actttaaagg ttctatggaa
     1921 gagtggcagg cgatgggcgt aatgaactat gaaatggaat ctgcaaccct gctgaccatg
     1981 tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa ccgcacccag
     2041 caagagatcc cgaatgctga gacgatgaaa caaaccgaaa gccatgcggt gaaaatcgtg
     2101 gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc gacgcgttcg
     2161 gccttttgta tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg acgtttgtac
     2221 agcacaattc tattttgtgc gggtaagttg ttgcgtcagg aggcgtggtg gatttctcaa
     2281 tcatggttta cgcagttatt gcgttggtgg gtgtggcaat tggctggctg tttgccagtt
     2341 atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt
     2401 taagcgcggc aaaacaacaa attacccaaa gcgagcactg gcgtgcagag tgcgagttac
     2461 tcaataacga agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg
     2521 aagtaaccac gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga
     2581 ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt atttttgagc
     2641 acagcaatcg ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc
     2701 tacgtgaaca actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac
     2761 aagaacgcca taccctgacc cacgaaattc gcaatctcca gcaactcaac gcgcaaatgg
     2821 cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc cagggcaact
     2881 ggggcgaggt agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat
     2941 atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg
     3001 tgcggctgcc gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg
     3061 aacgctattt taacgccgaa gacgactaca cccgcgaaag cgcgctacag gaacatatcg
     3121 cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc
     3181 tgcgaactct ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc
     3241 ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc
     3301 cgactacgct gctggtggcg ttgcgtacta ttgccaacct gtggcgttat gagcatcaaa
     3361 gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt
     3421 tcatcgatga catgtccgcg attggtcaaa gtctcgacaa agcgcaggat aactaccgcc
     3481 aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca gaagcgtttc
     3541 gcggtttagg agtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga
     3601 gccaggatga agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc
     3661 aacgcgatga tgaatataat cagcagtcgc gctagcccat taggggtagt taagccgggt
     3721 agaaatctag ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca
     3781 ggcattgaga tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg
     3841 aaggaacaaa aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat
     3901 gtcatgaatg atttgatgtc atttggtatt catcgtttgt ggaagcgatt cacgattgat
     3961 tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg
     4021 acagcgaaat tctcccgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat
     4081 gaatccatgc tcaaaatggg acgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt
     4141 gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc
     4201 accatttcgt ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat
     4261 cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag
     4321 ccgctgagca aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg
     4381 gtcgcgaacg acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat
     4441 caggataccc tgaaagccat gatgcaggat gccggattcg aaagtgtcga ctactacaat
     4501 ctgacggcag gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat
     4561 gccttttaaa cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg
     4621 ctcacccgcg ctgaaaacag cccgctcgcg tctgctgggt aaagtattgc gcgtggaggt
     4681 aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg
     4741 cgaatgggca ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact
     4801 tcgcgatcgc cagcagctta ccgcactgat tcgcagtggt gagctggaag tgcagggcga
     4861 tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg gcagagttcg accctgcgga
     4921 actgctggcc ccttataccg gtgatatcgc cgctgaagga atcagcaaag ccatgcgcgg
     4981 aggcgcaaag ttactgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat
     5041 tactgaagag tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac
     5101 ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac tggaggctaa
     5161 atgacgccag gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga
     5221 cttgatgaac tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca
     5281 ttattctgga tgccaaatcg gcataaagac aaacttttag gtgagcgact acgactggcc
     5341 ctgcaagaac tggggccggt ttggatcaag ttcgggcaaa tgttatcaac ccgccgcgat
     5401 ctttttccac cgcatattgc cgatcagctg gcgttattgc aggacaaagt tgctccgttt
     5461 gatggcaagc tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg
     5521 tggtttgacg attttgaaat caagccgctg gcttctgctt ctatcgccca ggttcatacc
     5581 gcgcgattga aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg
     5641 ccggtcatta aagcggatct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg
     5701 ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga aaagaccttg
     5761 attgatgaac tgaatttgct gcgggaatct gccaacgcca ttcagcttcg gcgcaatttt
     5821 gaagacagcc cgatgctcta catcccggaa gtttaccctg actattgtag tgaagggatg
     5881 atggtgatgg agcgcattta cggcattccg gtgtctgatg ttgcggcgct ggagaaaaac
     5941 ggcactaaca tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt
     6001 cgcgacagct ttttccatgc cgatatgcac cctggcaaca tcttcgtaag ctatgaacac
     6061 ccggaaaacc cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa
     6121 gataaacgct atctggcaga aaactttatc gccttcttta atcgcgacta tcgcaaagtg
     6181 gcagagctac acgtcgattc tggctgggtg ccaccagata ccaacgttga agagttcgaa
     6241 tttgccattc gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt
     6301 ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg
     6361 caactggtgt tactccagaa aaccctgctc tatgtcgaag gggtaggacg ccagctttat
     6421 ccgcagctcg atttatggaa aacagcgaag cctttcctgg agtcgtggat taaagatcag
     6481 gtcggtattc ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa
     6541 atgccagaac tgcctgaatt ggtttacgac agtttgcgcc agggcaagta tttacagcac
     6601 agtgttgata agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt
     6661 tattttctcg gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga
     6721 cctgaatggg ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc
     6781 ggttggcgca aaacacgctg attttttcat cgctcaaggc gggtcgtgta acgtataatg
     6841 cggccttgtt taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg
     6901 cagttattga ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc
     6961 atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa
     7021 ccaaagcagg ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag
     7081 caggcggata cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag
     7141 caggtgtaat ccgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc
     7201 ctcgtcgttc tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt
     7261 cgcgcgttgc gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc
     7321 caggagtttc aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacgccc
     7381 gaactgaaag cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac
     7441 gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg
     7501 aaagataatg aagctgcgca tgagggcgta acgcctgccg ctgcacaaac gcaggccagt
     7561 tcgccggaac agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct
     7621 gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag
     7681 aagatactca accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca
     7741 ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc
     7801 tggtatccgc gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg
     7861 tggcctcgcc gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag
     7921 cgccggtgat tctctatcag gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac
     7981 gtcgcctggt ggtgccgctg ctggtttcca gctctctgct gttttatatc ggcatggcat
     8041 tcgcctactt tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag
     8101 gggtgcaggt atccaccgac atcgccagct atttaagctt cgttatggcg ctgtttatgg
     8161 cgtttggtgt ctcctttgaa gtgccggtag caattgtgct gctgtgctgg atggggatta
     8221 cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg
     8281 ggatgttgct gacgccgccg gatgtcttct cgcaaacgct gttggcgatc ccgatgtact
     8341 gtctgtttga aatcggtgtc ttcttctcac gcttttacgt tggtaaaggg cgaaatcggg
     8401 aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg
     8461 tcagggcggt tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc
     8521 gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg
     8581 gctactcatc accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca
     8641 gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc
     8701 tgcgactgaa gaagcgatta ttgagctggc cgcgcagtca gaagtggtgg cgattggtga
     8761 atgtggtctc gactttaacc gcaacttttc gacgccggaa gagcaggaac gcgcttttgt
     8821 tgcccagcta cgcattgccg cagatttaaa catgccggta tttatgcact gtcgcgatgc
     8881 ccacgagcgg tttatgacat tgctggagcc gtggctggat aaactgcctg gtgcggttct
     8941 tcattgcttt accggcacac gcgaagagat gcaggcgtgc gtggcgcatg gaatttatat
     9001 cggcattacc ggttgggttt gcgatgaacg acgcggactg gagctgcggg aacttttgcc
     9061 gttgattccg gcggaaaaat tactgatcga aactgatgcg ccgtatctgc tccctcgcga
     9121 tctcacgcca aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca
     9181 acgtgttgcg cactggcgtg gagaagatgc cgcatggctg gctgcaacca cggatgccaa
     9241 tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact
     9301 gtgcttaatc tctttattaa taagattaag caatagcatg gagcgagcct caccatcggg
     9361 ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg gtaataatca ccttatcacc
     9421 cggataaggg gttgccggat cgacaatgtc tttcggttta tataccgata gctgatgaat
     9481 caccgccgat gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg
     9541 ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca aattcgacaa acagatagtt
     9601 gggaaacaat ggctcactga ctgcagtacg ttttccacgc acgatttttt ccagggtgat
     9661 catcggtgcc aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg
     9721 cccgcgcttg cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg
     9781 ggcgcaagga tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac
     9841 cgtcagagtt tacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat
     9901 gggcgtagat taagaggcta caatggacgc catgaaatat aacgatttac gcgacttcct
     9961 gacactgctt gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg atccgcatct
    10021 ggaaatcact gaaattgctg accgcacttt gcgtgccggt gggcctgcgc tgttgttcga
    10081 aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt
    10141 ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt gaagttggta aattattggc
    10201 gtttctgaaa gagccggagc cgccaaaagg tttccgcgac ctgtttgata aactgccgca
    10261 gtttaagcaa gtattgaaca tgccgacaaa gcggctgcgt ggtgcgccct gccaacaaaa
    10321 aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc attatgacct gctggccgga
    10381 agatgccgca ccgctgatta cctgggggct gacagtgacg cgcggcccgc ataaagagcg
    10441 gcagaatctg ggcatttatc gccagcagct gattggtaaa aacaaactga ttatgcgctg
    10501 gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc atccgggcga
    10561 acgtttcccg gtttctgtgg cgctgggtgc cgatcccgcc acgattctcg gtgcagtcac
    10621 ccccgttccg gatacacttt cagagtatgc gtttgccgga ttgctgcgcg gtaccaagac
    10681 cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg agattgtgct
    10741 ggaagggtat atcgaacaag gcgaaactgc gccggaaggg ccgtatggcg accacaccgg
    10801 ttactataac gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga
    10861 agatgcgatt taccattcca cctataccgg gcgtccgcca gatgagcccg cggtactggg
    10921 cgtggcgttg aacgaagtgt ttgtaccgat tctgcaaaaa cagttcccgg aaattgtcga
    10981 tttttacctg ccgccggaag gctgttctta tcgtctggcg gtagtgacga tcaaaaaaca
    11041 gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat
    11101 gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac gcacgcgact ggaacgatgt
    11161 gatttgggcg attaccaccc gtatggaccc ggcgcgggat actgttctgg tagaaaatac
    11221 gcctattgat tatctggatt ttgcctcgcc tgtctccggg ctgggttcaa aaatggggct
    11281 ggatgccacg aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa
    11341 agatccagat gttgtcgcgc atattgacgc catctgggat gaactggcta tttttaacaa
    11401 cggtaaaagc gcctgatgcg cgtttgtttt gccctattta tcgatccgac agagaaagcg
    11461 catgacaacc ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg
    11521 tgtccgcatc gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt
    11581 gatggatgag cgcgacaaac gtccgttctc aatggcctcg acgccggatg aaaaagggtt
    11641 tatcgagctg catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg
    11701 catcctcaaa gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga
    11761 tgatgaagag cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc
    11821 gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg
    11881 gcgtgaagag cagcatctgt atgatctctg cgagcttgag gcgctttcgt taaagcaccc
    11941 tggtctgcaa gtggtgccgg tggttgaaca accggaagca ggctggcgtg ggcgtactgg
    12001 taccgtgtta acggcggtat tgcaggatca tggtacgctg gcagagcatg atatctatat
    12061 tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg ttttgcagtg agcgtaatgc
    12121 gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaaacccgc
    12181 ccctgacagg cgggaagaac ggcaactaaa actttattca gtggcattta gatctatgtc
    12241 gcatccggca attggtgcac gacgcctgat gcgacgctgg cgcgtcttat caggcctaca
    12301 ttggtgccgg atcggtagac cggataaggc gttcacgccg catccggcaa gtggttaaac
    12361 ccgctcaaac accgtcgcaa taccctgacc cagaccgata cacatcgtcg ccagaccaaa
    12421 ctgaacgtct ttgcgttcca tcagattcag cagcgtggtg ctgatacgcg caccggaaca
    12481 gcccagcgga tgacccagcg cgatcgcacc accattgagg ttgatcttct cgtcaatctg
    12541 ctccattagc ccgagatctt taatgcacgg caggatctgc gcggcaaagg cttcgttcat
    12601 ctcaaacacg ccgatatcgc tggcagaaag ccccgctttt ttcagcgcca gcttcgaggc
    12661 cggaaccggg ccgtaaccca taatcgatgg gtcacaacca acgaccgcca tcgaacgcac
    12721 acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt tcactcatca ccagcatggc
    12781 agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt accgtaccgt ttactggatc
    12841 aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc
    12901 gtaattaaac tgcttcagga cgccgtcggc atcgtgacca ccagttggga tgatttcatt
    12961 tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg tgtgaccgcg cggcaaaggc
    13021 atcctgcatt tcacggctga taccgtgcat acgcgccagc atttctgccg ttaagcccat
    13081 catgcccgcc gctttggcga cattgcggct caggccgggg tgaaaatcga cgccgtgact
    13141 catcggcaca tggcccatat gctccacgcc gccaaccaga catgcctgcg catcgccagt
    13201 catgatcatt cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca agcgattgac
    13261 ggtaaccgcc gggacagagt gcggcacttc tgccagcagc gccgcgttac gggcgatatt
    13321 aaaaccctgc tccagcgtct gctgcacaca accccagtaa atatcgtcga gggccgccgc
    13381 ttccagcgcc gggttacgcg ccagcaggct acgcattaaa tgagcggaga gatcttctgc
    13441 acgcacgtta cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac
    13501 aatgacaacc tgttccattg tgactcctta agccgttttc aggtcgccaa ccggacgggc
    13561 tggctcaacc ggaggatagt acggttcgtt atgacgcgct ttattacgca gaccttccgg
    13621 cacttcatac agcgggccga ggtgctgata ttgctgtgcc atatcgaggt attttgcgct
    13681 accgagggtg tccagccagc ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta
    13741 gaccagcgcc atatccgctt ccgccggagt ggcgataatg ccttcctcca gacagcgcac
    13801 cacttcgttg accatcggga tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg
    13861 cttcggctgg ctcacttctg ccagcaggtc gtcaacagcg gcgtcttctt ctttcttcgg
    13921 cttaccttta ctgtcttctt tataacgcca gaaaccgagg ccgttcttct gaccaaaacg
    13981 gttggcatca aacagcgcat cgatggcatc gcggtaatct ttctgcatcc gctgcgggaa
    14041 gcctgctgcc atcaccgcct gagcgtgatg cgcggtgtca atgcccacga catccagcag
    14101 atatgccgga cccatcggcc agccaaactg tttttccatc actttgtcga tcttgcggaa
    14161 atccgcgccg tcgcgcagca actggctgaa accggcgaaa tacgggaaca gcacgcggtt
    14221 gacgaagaag ccggggcagt cgttaaccac aatcggcgtc ttgcccatct tgctcgccca
    14281 ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg ccgcgaataa tttctaccaa
    14341 cggcatccgg tgtaccgggt taaaaaagtg cattccgcag aagttttccg ggcgttccag
    14401 cgcgttggcc agttcgctga taggaatggt tgaagtgtta gacgccagca cggtatccgg
    14461 gcgtactttt tgttcggttt ctgccagtac ggcttttttc actttcgggt tttcgacaac
    14521 cgcttctacc acaacatcca cgcggtcaaa cccggcgtag tcgagcgttg ggtggatcgt
    14581 ggagatcacg ccagccagtt tcagaccatc gatcttgccg cgttcaagct gcttgttcag
    14641 cagtttcgcg gcttcggtca tgccgagggt taacgacttg tcgttgatat ctttcatgac
    14701 aaccggtacg cctttccacg cagactggta agcaatgccg ccacccatga tgcctgcgcc
    14761 cagtacagcg gcctgtttcg gtgtttcaac gtctttggtg agtttcttcg ctttgccttt
    14821 tacatattga tcgttaagga aaatgccgac cagtgcgcgg gcttcgttgg tgtgcgccag
    14881 cgggacaaag cttttgtttt ccagatttaa tgcttcttca cgaccaaaac gggccgcagc
    14941 ttcaatggtt tttactgcgg tgatgggcgc cggataatgt ttccccgccg tttgtgcgac
    15001 catcccttta gcgatggtga agctcatggt ggcttcaatc ttgctcagtt ttagtggttc
    15061 cagcttcggc tgacgttttg ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaac
    15121 cgcctttgcg ccttcaacca gtttttctgc tttgactacg ccatccacca gaccgatttt
    15181 cagcgcctga tccgcgccga catctttacc ggcggcaatg atttccagcg cactgtcagc
    15241 gcccagcata cgtggcatac gtacagaacc gccaaagccc ggcatgatgc ccagtttggt
    15301 ttccggcaga ccgatgcgca gatccggcgt cgccagacga taatcggtcg ccagcacgca
    15361 ttcgcagcca ccgcccagcg catagccgtt gacggcagca atggtcggca ccggcagatc
    15421 ttccaggcga ttaaacacgc tattggcaaa gtgcagccac tgacttaact gttcttcagg
    15481 aacgaggaac agggacaaaa attcggtgat atcagcaccg acgataaagg ctgctttgtt
    15541 cgaacgcagc agcagccctt ttagatctga ttgctgttcc agcacgccga tggcctcgcc
    15601 gaggctggcg acggtcgcag tgtcgagttt attaactgaa cctggggcat caaataccag
    15661 ttcggcaatg ccatcttcca gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc
    15721 agtctcctga atccgcaagg ttatctggtc gtaccagatg catcgaagtg tgtattttgt
    15781 gttaaaaata tgcaaataaa agattaaaga aatgccgatc tgatcacgct cggcagaaat
    15841 cacgctctgg atgaacgatg tgctaagatg cggagactta aggtcaaaaa aacagaaggg
    15901 taaaaaatgg aatcactggc ctcgctctat aaaaatcata tagctacctt acaagaacgg
    15961 actcgcgatg cgctggcgcg cttcaagctg gatgcattac ttattcactc cggcgagctg
    16021 ttcaacgttt ttctcgacga tcatccctat ccgtttaaag tgaacccgca attcaaagcg
    16081 tgggtgccgg taactcaggt gccaaactgc tggttgctgg tggatggcgt gaacaagccg
    16141 aaactgtggt tctatctgcc ggttgattac tggcacaatg tcgaaccgct gccgacctcc
    16201 ttctggactg aatatgtgga agtgatcgca ctgccgaaag ccgatggcat tggtagcctg
    16261 ctgcccgctg cgcgcggcaa tatcggttat atcggtccgg tgccggaacg tgcgctgcaa
    16321 ctgggtattg aggccagcaa catcaacccg aaaggggtga tcgactacct gcattactac
    16381 cgctccttca aaaccgagta cgaactggcc tgtatgcgtg aagcgcagaa aatggcggtc
    16441 aacggtcatc gcgcggcaga agaagcgttc cgttctggca tgagcgagtt cgatatcaac
    16501 attgcctatc tgaccgcgac cggtcatcgt gataccgacg taccttacag caacattgtg
    16561 gcgcttaacg aacacgctgc ggtgctgcat tacaccaaac tggaccacca ggcaccggaa
    16621 gagatgcgca gcttcctgct ggatgccggg gccgaatata acggctatgc ggctgacctg
    16681 acccgtacct ggtcggcaaa aagtgacaac gactacgcac agctggtgaa ggacgttaat
    16741 gatgaacaac tggcgctgat cgctaccatg aaagcaggcg tcagctatgt ggattaccac
    16801 atccagttcc atcagcgcat cgccaaactg ctgcgtaaac atcaaatcat caccgatatg
    16861 agtgaagaag cgatggtcga aaacgatctc accggaccgt ttatgccgca cggtatcggt
    16921 catccgctgg gcctgcaggt gcatgacgta gccggtttta tgcaggatga tagcggtaca
    16981 cacctcgcgg caccggcaaa atatccgtac ctgcgctgca cccgtattct ccagccgggt
    17041 atggtgttaa ccatcgaacc gggtatctac ttcattgaat cgctactggc accgtggcgt
    17101 gaagggcagt tcagcaagca cttcaactgg cagaaaattg aagcactgaa accgttcggc
    17161 ggcattcgta tcgaagacaa cgtggtgatc cacgaaaaca acgtggaaaa catgacccgg
    17221 gatctgaaac tggcgtgatg gaaagctggt taattcctgc ggcaccggtc acggtcgttg
    17281 aagagatcaa aaagagccgc ttcataacac tgttggcgca taccgatggc gttgaggcgg
    17341 cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga tgcccgccac cattgcgtgg
    17401 cgtgggtggc gggtgcgccg gatgattctc aacagctggg tttctctgac gacggggagc
    17461 cggcaggaac ggcaggtaaa ccgatgctcg cccagctaat gggcagcggc gtcggggaaa
    17521 ttaccgctgt ggtagtgcgc tactacggtg gcatattgct tggcaccggt gggttagtga
    17581 aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa cgcaagacgc
    17641 cattaaccga atatactttg caatgtgaat atagtcagtt aaccggcatt gaagcgttgc
    17701 tggggcagtg tgacggcaaa attatcaaca gtgattatca ggcattcgtt ctgctgcggg
    17761 tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa gctggcggat tttagccgtg
    17821 gttcattgca attgttagcg attgaagaat aatccccact tcgttttgca gaactaagga
    17881 agcggcagag atgcattttc gcgccattac ccgaatcgtt ggactactgg tcatcttatt
    17941 ttcagggacc atgattatcc ctgggctggt agcactcatc taccgggatg gagcgggccg
    18001 cgcttttacc cagacctttt ttgtcgccct cgccattggt tctatgctgt ggtggccgaa
    18061 ccgtaaagag aagggcgaac tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg
    18121 gaccgtgctg ggtagcgtcg gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac
    18181 gattaccgat gcgttttttg aatctttctc tggcctgacc accaccgggg ccactacgct
    18241 ggtggggctg gattcgctcc ctcatgccat cctcttttat cgccagatgc tgcaatggtt
    18301 tggcgggatg gggatcatcg tgttagcggt tgcgatactg cctatcctcg gcgtgggtgg
    18361 gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg
    18421 aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc
    18481 gctggcgttg tggtttgccg gaatggatgc ctttgatgcc atcggccata gctttgcgac
    18541 tatcgctatt ggcggcttct cgacacatga tgccagtatc ggttatttcg acagcccgac
    18601 tattaacact atcattgcta tcttcctgct gatctccggc tgtaactacg gtctgcactt
    18661 ttcactgtta agtgggcgta gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt
    18721 tatcggcgta cagtttacgc tggtggttat ttgtacactc gtactgtggt ttcataatgt
    18781 ctacagttcg gcgctgatga caattaacca ggcgtttttc caggtggtat cgatggcgac
    18841 aaccgccggg tttacgactg acagcattgc ccgctggccg ctctttttgc cggtactgct
    18901 tttatgttca gcttttatcg gcggttgcgc cgggtcaacg ggcggtggcc tgaaagtgat
    18961 ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa
    19021 cgccgtctat agcattaagc tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt
    19081 ttggggattt ttctccgcct atgcattggt gtttattgtc agtatgctgg cgattatcgc
    19141 cacgggcgtg gatgactttt ctgcctttgc ctcagttgtt gcgacattga ataacctggg
    19201 gccagggctt ggcgtggttg ctgataactt taccagtatg aacccggtgg ctaaatggat
    19261 cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt acattgctgg tgctctttac
    19321 cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa cattaattct tttctcaaca
    19381 agggacggac aaacgcgcga gattgcctcc tacctggctt cggaactgaa agaactgggg
    19441 atccagacgg atgtcgccaa tgtgcaccgc attgaagaac cacaatggga aaattatgac
    19501 cgtgtggtca ttggtgcttc tattcgctat ggtcactacc actctgcgtt ccaggaattt
    19561 gtcaaaaaac atgcgacacg actgaattcg atgccgagcg ccttttattc cgtaaacctg
    19621 gtggcgcgca aaccggagaa gcgtactcca cagaccaaca gctacgcgcg gaagtttctg
    19681 atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg ccggggcgct gcgttaccca
    19741 cgttatcgct ggtacgaccg ttttatgatc aagctgatta tgaagatgtc aggcggtgaa
    19801 acggatacgc gcaaagaagt tgtctatacc gattgggagc aggtggcgaa tttcgcccga
    19861 gaaatcgccc atttaaccga caaaccgacg ctgaaataag cacaaagaat aaaaaatgcg
    19921 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    19981 caccactgac acggaacaac ggcaaacacg tcgccgggtc ag
//
LOCUS       DAJSKJ010000062        19830 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000062 DAJSKJ010000000
VERSION     DAJSKJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.062"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            106..648
                     /locus_tag="ON345_001940"
     CDS             106..648
                     /locus_tag="ON345_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072004.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            748..1950
                     /locus_tag="ON345_001941"
     CDS             748..1950
                     /locus_tag="ON345_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR3072005.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2170..2952
                     /locus_tag="ON345_001942"
     CDS             2170..2952
                     /locus_tag="ON345_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR3072006.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACTRDISRLLGWKSPFDLQAS"
     gene            2967..4172
                     /gene="rhmD"
                     /locus_tag="ON345_001943"
     CDS             2967..4172
                     /gene="rhmD"
                     /locus_tag="ON345_001943"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCR3072007.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVTDMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4229..5526
                     /locus_tag="ON345_001944"
                     /pseudo
     CDS             4229..5526
                     /locus_tag="ON345_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5535..6338
                     /gene="yfaU"
                     /locus_tag="ON345_001945"
     CDS             5535..6338
                     /gene="yfaU"
                     /locus_tag="ON345_001945"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR3072008.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPCGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRINGSY"
     gene            complement(6379..7281)
                     /locus_tag="ON345_001946"
     CDS             complement(6379..7281)
                     /locus_tag="ON345_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR3072009.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDEPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7475..8665)
                     /gene="glpC"
                     /locus_tag="ON345_001947"
     CDS             complement(7475..8665)
                     /gene="glpC"
                     /locus_tag="ON345_001947"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR3072010.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8662..9921)
                     /gene="glpB"
                     /locus_tag="ON345_001948"
     CDS             complement(8662..9921)
                     /gene="glpB"
                     /locus_tag="ON345_001948"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR3072011.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQHAGGQQ"
     gene            complement(9911..11539)
                     /gene="glpA"
                     /locus_tag="ON345_001949"
     CDS             complement(9911..11539)
                     /gene="glpA"
                     /locus_tag="ON345_001949"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR3072012.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11812..13170
                     /gene="glpT"
                     /locus_tag="ON345_001950"
     CDS             11812..13170
                     /gene="glpT"
                     /locus_tag="ON345_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR3072013.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13175..14251
                     /gene="glpQ"
                     /locus_tag="ON345_001951"
     CDS             13175..14251
                     /gene="glpQ"
                     /locus_tag="ON345_001951"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3072014.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YKKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14293..14535)
                     /locus_tag="ON345_001952"
                     /pseudo
     CDS             complement(14293..14535)
                     /locus_tag="ON345_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14714..15364
                     /gene="inaA"
                     /locus_tag="ON345_001953"
     CDS             14714..15364
                     /gene="inaA"
                     /locus_tag="ON345_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR3072015.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     LTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15418..15672)
                     /gene="yfaE"
                     /locus_tag="ON345_001954"
     CDS             complement(15418..15672)
                     /gene="yfaE"
                     /locus_tag="ON345_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR3072016.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESLNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15672..16802)
                     /gene="nrdB"
                     /locus_tag="ON345_001955"
     CDS             complement(15672..16802)
                     /gene="nrdB"
                     /locus_tag="ON345_001955"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR3072017.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17036..19321)
                     /gene="nrdA"
                     /locus_tag="ON345_001956"
     CDS             complement(17036..19321)
                     /gene="nrdA"
                     /locus_tag="ON345_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR3072018.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4585 a         4877 c         5404 g         4964 t
ORIGIN      
        1 aataagtgac tagatccctt ttttcgctca ttgaatcctc tgtgccaggc agtacaatcc
       61 cgcagctttg ggaattttga ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg
      121 ctagcaggtc tggccggaat gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc
      181 ggccaggcgg gtaaagaata caccaatatt ggtgtcggtt ttgggactga atcgacgggc
      241 ctggcgttaa gcggtaactg gacacataac gacgacgacg gtgacgtcgc gggcgtgggg
      301 ctggggttga atctgcctct cgggccgtta atggcgaccg ttggcggaaa aggcgtttac
      361 accaacccga attacggcga tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg
      421 aaaattggca acagcttccg tttgtttggc gagtattact actctccgga ttcgctctcc
      481 agcggtattc aaagttatga ggaagcgaat gctggcgcgc gttacaccat tatgcgtcca
      541 gtcagtattg aggcgggtta tcgctacctg aatctgtcgg gtaaagacgg taaccgcgac
      601 aacgccgtgg ctgacggccc gtatgttggg gttaacgcca gtttctgatc ccttccggcg
      661 cggcattctg tcgcgccatt cagtttctta ctacgcctta tctccctttg cgttatagtg
      721 attcgataac aaaagcagga gataaccatg ttaaaagtgg aaatgttatc caccggggat
      781 gaagtgttac acgggcaaat cgttgacact aacgctgcct ggctggccga ttttttcttt
      841 catcaggggc tgccattatc tcgccgcaat acggtggggg ataaccttga tgacttagtc
      901 accattcttc gcgaacgtag tcagcacgcc gatgtgctga tcgttaacgg cgggctggga
      961 ccgaccagcg atgatttaag cgcactcgcc gctgcgacag caaaaggtga aggcctggtg
     1021 ctgcatgaag cctggcttaa agagatggaa cgctatttcc acgaacgtgg acgagtaatg
     1081 gcaccgagca accgtaaaca agcggagctg cctgccagtg ctgaatttat caataacccg
     1141 gtaggcaccg cctgtggttt tgccgtgcag cttaatcgtt gcctgatgtt ctttactccc
     1201 ggcgtaccgt cagaatttaa ggtgatggtc gagcacgaaa tcctgccgcg cctgcgcgag
     1261 cgtttttctt taccacagcc gccggtttgt ctgcgtttaa ctacctttgg tcgttcggaa
     1321 agcgatctgg cacaaagcct ggacactcta caactgccgc cgggcgtaac aatgggctat
     1381 cgctcctcaa tgccgatcat cgaactgaaa ctcaccggac cggcaagcga gcaacaggcg
     1441 atggaaaaac tgtggctgga cgttaaacgt gttgccggac agagcgtgat tttcgaagga
     1501 accgaaggac tgcccgcgca gatcagtcgc gaattgcaaa accgccagtt cagcctgacg
     1561 ttgagcgagc aattcaccgg tggtttattg gctttgcaac tttctcgcgc aggtgctcca
     1621 ttgctggcgt gtgaagtggt tccttcacag gaagaaaccc tggcgcaaac tgcgcactgg
     1681 attacagaac ggcgggccaa ccattttgcc gggctggcac tggctgtttc gggtttcgag
     1741 aacgagcatc tcaactttgc gctagccacg ccagacggca ctttcgctct gcgtgtgcgt
     1801 ttcagcacta cacgctacag cctggctatc cgtcaggaag tgtgcgcaat gatggcactg
     1861 aatatgctgc gccgttggtt aaacggccag gacatcgcca gtgagcatgg ctggattgag
     1921 gttgttgagt ccatgacctt atctgtctga ttccgccact ctgtgccggt ttactgcacc
     1981 ggcacattcc ccctccggca aaatttgtcc aacctgttgt actcaataag aaaaaatccg
     2041 tgcgctgatt cacaaatttg tgcgtaattt ctccagtccg ctggacgagg agcgggattt
     2101 gatgcgcttc acaattttac tccccctgag caacgagact gattccagta tctaagactg
     2161 gagtccagta tgttggaatc aagcaaagtt cccgccctga cacgggctat tgatattctc
     2221 aatttgattg cccgcattgg cccctgtagt gctgcgacga tcattgacac attgggaatc
     2281 cctaaaagca cggcctattt gctgcttaat gaactcaggc gtcagcgttt tcttagcctt
     2341 gatcaccagg aaaatttttg tttgtggacc aggctggtgg agctttctgg tcatgcgtta
     2401 agcaagatgg atctccggga actggctcga ccgcgtttga cgcaactgat ggataccacc
     2461 gggctgttat gtcacctggg gattattgat aacggcagcg cttattacat tttaaaagtg
     2521 gagtcatcag ccactatcag cgtgcgttcc catgaaggaa aaagcctttc gctttatcgc
     2581 tccggaatag gtaaatgcct gcttgcctgg caacctgcag cggtacagca aagtattatc
     2641 gaagggctgg tctgggagca ggcgacaccc acaacgatta ctcatccaca gcaacttcac
     2701 gaagaactgg cgcgtattcg ccgtcagggc tggagctatg acaacggcga agattacccc
     2761 gatgtccgtt gtgtcgctgc gccggtgttt aacgccaata acgagcttac cgctgcgatc
     2821 tcggtggtcg gtacccgctt acaaatcaat gaagaatacc gtgattatct ggcgggcaag
     2881 gccattgcct gcaccaggga tatttcacgt ctgttgggat ggaaaagtcc atttgactta
     2941 caggcctcat aacaatggag aacatcatga ccctaccaaa aatcaaacag gttcgcgcct
     3001 ggtttactgg cggtgcgaca gcagaaaaag gtgctggcgg cggtgattat cacgaccagg
     3061 gggcgaatca ctggattgac gatcatattg ccaccccgat gagtaaatac cgcgattacg
     3121 agcagtcacg ccagtcattt ggcattaacg ttcttggcac gttgattgtt gaagtcgaag
     3181 cagaaaacgg ccagaccgga ttcgctgttt cgacagccgg tgaaatgggc tgttttattg
     3241 tcgaaaaaca tcttaaccgt ttcattgagg gtaaatgtgt cagtgatatc aaactgatcc
     3301 atgatcaaat gctcaatgcc accctgtatt actccggctc tggtggcctg gtgatgaaca
     3361 cgatttcctg tgtcgatctg gctctgtggg atctgttcgg caaagtggtc gggcttccgg
     3421 tttataaact tttaggcggc gcagttcgtg atgagattca gttctacgcc acaggcgcgc
     3481 gtccggatct ggcaaaagag atgggcttta tcggtggcaa aatgccgacg cactgggggc
     3541 cacatgatgg cgatgcgggg atccgcaaag atgccgctat ggtcacggat atgcgtgaaa
     3601 aatgcggtga ggatttctgg ttaatgctcg actgctggat gagtcaggac gtgaactatg
     3661 ccaccaaact ggcccacgct tgcgcgccct ataacctgaa atggatcgaa gagtgcctgc
     3721 cgccacagca gtatgaaggt tatcgcgaac tgaaacgcaa cgcgccagtc ggaatgatgg
     3781 tcaccagcgg tgagcaccac ggcacactgc aatcttttcg tacgctttca gaaaccggta
     3841 tcgacattat gcagccggat gttggctggt gcggtggttt aaccacgctg gtggaaattg
     3901 ccgcaatcgc caaatccagg gggcaactgg tggtgccgca cggttcgtct gtttactctc
     3961 accatgcggt gatcaccttc accaatacgc cattcagcga attcctgatg accagcccgg
     4021 attgttcaac gatgcgtccg cagtttgacc cgattctgct caatgagccg gttccggtga
     4081 atggtcgtat tcataaatca gtgcttgata aacccggttt tggcgtcgaa ctcaatcgtg
     4141 actgcaattt gaaacgcccc tacagccact aatcactccg ggcgttgctg tgacaacggt
     4201 aacgcccaca tattctaagg agctttttat gagcaccgct ttgcttgacg ccgtggtgaa
     4261 gaaaaaccgt gtgcgtttaa ttccgtttat gttggcgctg tatgtgctgg cgtttctcga
     4321 ccgttcgaat atcggttttg ccaaacagac ctaccagatt gataccgggt tgagtaatga
     4381 agcttatgcg ctgggagcag gcattttctt tgtggtatat gcgtttctgg gtgttccggc
     4441 gaatcttttg atgcgcaaac tgggggccag aacctggatt ggtacgacaa cactgctgtg
     4501 gagatttctt tcggcagcca tggcatgggc cgatactgaa gcgaaatttc tgatagttcg
     4561 cactctgctt ggtgctgcgg aggccggatt tttccctggt atgatttatc tcacctcgca
     4621 atggtttccg cagcgtaatc gcgccagcat tatggggctg ttctatatgg gcgcaccgct
     4681 ggcgttaaca ctgggatcac cgctttccgg cgcgctgttg gagatgcatg gatttatggg
     4741 gcatcccggc tggttctgga tgtttgtgat tgaaggattg ttggcagtcg gtgctggggt
     4801 attcacattc ttttggcttg atgacacacc ggagcaggca cgttttctga gtaaacaaga
     4861 aaaaacgttg cttatcaatc aactggcaag tgaagaacaa cagaaagtga cttctcggct
     4921 gagcgatgcg ctgcgtaatg gccgagtctg gcaactagcg attatctacc tgaccattca
     4981 ggtggcggtt tacggattaa ttttcttcct gccgacccag gttgcggcat tgctgggaac
     5041 aaaagtgggc tttacagcgt cggtggtcac cgccattccg tgggttgcgg ccttgtttgg
     5101 gacctggctt attccgcgct attccgataa aaccggcgaa cggcgtaatg tcgcagcgct
     5161 gacattactg gcggcgggca ttggtattgg tctgtccggg ctgctttctc cagtaatggc
     5221 gatcgtagcg ctgtgtgttg cagctatcgg gtttattgcc gtgcagccgg tgttctggac
     5281 gatgccgaca caactgcttt ccggtacggc gctggctgcg gggattggtt ttgtaaacct
     5341 gtttggtgca gtggcgggtt tattgccccg atcctgcgcg tgaaagcaga aacgttattt
     5401 gccagcgatg cggcaggatt actgacactg gcagcggtgg cggtcatcgg ttcgctgatt
     5461 attttcactc tgcgtgtaaa tcgtactgtt gcgcagaccg acgtggcaca tcattacata
     5521 ggttaaggaa cacgatgaac gcattattaa ccaatccctt taaagaacgt ttacgcaagg
     5581 gcgaagtgca aattggtctg tggttaagct cgactacttc ttatatggca gaaattgccg
     5641 ctacttccgg ttatgactgg ttgctgattg acggggagca cgcgccaaac accattcagg
     5701 atctttatca ccagctacag gcggtagcgc cctatgccag ccaacccgtg atccgtccgg
     5761 tggaaggcag taaatcgctg attaaacaag ttctggatat tggcgcgcaa actctattga
     5821 tcccgatggt cgatactgcc gatcaggctc gtcaggtggc gtctgccacg cgctatcctc
     5881 cctgcggtga gcgtggtgtt ggggccagtg tggcacgggc tgcgcgctgg ggacgcattg
     5941 agaattacat ggcgcaagtt aacgattcac tttgtctgtt ggtgcaggtg gaaagtaaaa
     6001 cggcactgga taacctggac gagatcctcg acgtcgaagg gattgatggc gtgtttattg
     6061 gaccagcgga tctttctgcg tcgttgggct acccggataa cgccgggcac ccggaagtgc
     6121 agcgaattat tgaaaccagt attcggcgga tccgtgctgc gggtaaagcg gctggttttc
     6181 tggctgtggc tcctgatatg gcgcagcaat gcctggcgtg gggagcgaac tttgtcgctg
     6241 ttggcgttga cacgatgctc tacagcgatg ccctggatca acgactggcg atgtttaaat
     6301 caggcaaaaa tgggccacgc ataaacggta gttattgata tcaaaggccc atggggatcg
     6361 gctgtgggcc tgtgttaatt agtggttatt cgctgccaga tcggcttcgc ttagctgggt
     6421 ggccgcgagc acctggtcac gatcaatgcc ctgttccagc atgcgcaaag caattttaat
     6481 ggcttgttca tgcatacctt cctgccagcc aatttgatgc ccttcctgcc gtagccgttc
     6541 agcaatagtc attaatctct ccttttgtag tggtgaacgt tcggcaatct cctcaataaa
     6601 acgggtgaaa cgggaggtat cgccgcattg cagcagataa ttaaacagtg tttgtagctg
     6661 gctgtcatta gtgaagcctc taaccaaaag cgtggtgatc ctgtcgacca tgccgattaa
     6721 atcgcggtcg cgaatatgct tttgaatcag ttccagcaga gctatacgcc gatgttgcat
     6781 gatctcatcg tcaggtacga tggtgatatc caccaacgga aaagcttcgg tgtacaactg
     6841 ccgggcaagt tgcggctcgt caaactcatc cagccagtta agtgagtacg ggtagggcga
     6901 tgtttcgcca tgataaaaca gcaatggcac caccagcgga actctgtcat agcctttatc
     6961 caggtgacgc tgcatggcgg cagtggcata gcgcattagc cgaaaagcca tattcttttc
     7021 tgcagagctt tgatgttcaa tcacgcagta gatatagccg tcaccgtcgc tggtttccac
     7081 ggaccacaaa acatccgagt agtaagcgcg taaacttttt tcaataaaac tagtgggttc
     7141 caggcgtaag gtttgcaggt tgcaaagctt gcgcagtggt tctggtaaat gtatttcgag
     7201 aaaatcccgt gcggtttcgg gtgtgaacat aaaggtttta aacaccgcat catgcgggga
     7261 ggatgttgtt gattcggtca tggttgtccg tcacctgata tttatgatga cgcgacatta
     7321 cgtctatccc gggcctttga catctgtcag tttcagcctt tgcgagacat tttcagagga
     7381 attttcaaag cgttgcaacg catacatggt gcgtggaatc gcgctcgcaa aaatgcccga
     7441 taagcaatgt gcttaccggg catcaaaagg tcggttaagc cagcgcctgg gccagtagcg
     7501 taatcggatg ttcgcagcga aggctcgtgg acatctcaat ctgccatttg caggtttcgc
     7561 agtcggtgat gaccagatcc gcgccgctct cttctatctg gcggaacagt ggcgcaccga
     7621 tggcttgtga ggtggggtag ttctcttttt tgaaaccgta agtacccgca ataccgcagc
     7681 actgggaatc cagcaccgtt aactcaagcc ccgggatttt acgcaacagc tccagggtgt
     7741 agagcgtcca gcccattttt tccatatggc acggagtgtg ataaaccact ttcagcggca
     7801 gcggtttcag cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag cgggttgcca
     7861 gttcgatatg atcgcgcaag cctttgttgt cgacattcag cacttccggg tattcgtcgc
     7921 gcagggcaaa tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt actcccacag
     7981 cttcgcggat cgactctaca ttcgtaattg cctgtttgcg tgctttatcg gtaaagccgt
     8041 tggcgattag cggtacgccg cagcattttt ctttgctgag cagttgtaca ccggtaccca
     8101 ttgcgttgag cactttaatt aaatctttac ctaactgcgg atggttgtag ttaacgaagc
     8161 agccgtgaaa gaaagcgacc tggtctttat attgtgcttg ctgagccgct atgctgcgat
     8221 accagcgacg gaacgtgccg aaggagtatt tcggtagcgt gcggcgatga tcgattttta
     8281 acgccgcatc aagcagctgc cgcaccggtt tcagcgaggt agcggtgttg acgattggtg
     8341 cgaacggcgt ggaaacgcta cccatcaggt cggtatgact caacacaaaa ttacgcagcg
     8401 acgggcgcgt ggtgtcatat tttgcccgcg cgcgctggat aatatcgcca atcttcacat
     8461 cggacgggca ggcgacttca caacgtttgc agttgatgca atatttcagc gcctcgtcat
     8521 acagtgcgcc atctttcaaa cgcagacgct cgccatccgg cccggcttgt tttggccctg
     8581 gataaccggg attcacccgg ctcaccgggc aggcggtggt gcagacggtg cacttaatgc
     8641 agttttcgaa gctggtgtcg ttcattgttg gcctcctgcg tgttgggcaa tctgttgagc
     8701 ggcatgtaaa gcactgacgg cacaaacacc gccgccgcat ccctgggcga tgggatcaaa
     8761 tccgcccagc accgaaccga tggcaaacag gttctcaatg gtttgccctg cctgcgacgg
     8821 gcgtagcgtc tcatcagtgg ttacaccgaa ctgctgccac ggttgcggcg caaaaaaatc
     8881 gcccttatac cattcacccc gcgtggcggt ttgtagcaca tcaaggccga gaatcggctc
     8941 gcgaatgccg ttacgttccg ctaccagtcc gccactaaag aaactgccgc tggcgagaac
     9001 cgcgaaacgt ggacgtagcg gaatatcggc gtgattgcgg gtccagattt cattcactac
     9061 gccattttta caggtcactt ttttcacttc atcgcccggc atccacacgc caccctggcg
     9121 cacaaactgg cgctgtaact ggttttgcag acgaatgccc agcacggaag gcggcagcgt
     9181 tggcaaaagc atcagtgaac aaggtagttt ttcattcaac caacgccaca gtttgtcatc
     9241 ggccaggccg aagcaggcgg gcatcaggat catttcgcag gtattggcga caggaataag
     9301 cgcatcaagt aacagcggcc agttttcttc attatcaagg aaacgggcga tattcaccgc
     9361 gcgaaattcg gtggcgttat tgcgcaggac atccagttcc ggcagctcta tttctgcggt
     9421 ttcaaccgaa agatccagtt cgcgcaacga agccgccgca agatgcgcct ggaaatccat
     9481 cagcccgctt atccccacta cacatatctt cttcgcgggt agcggccaga cgggaacttc
     9541 tggcgaactt aaccaggttg cacgcaaagt gccgagcggg gtaacgcgct gatgggccag
     9601 ttccacgctg ccctgcaatt gcgcaccgct ttcagcgatc aatgcctgcg cctggcaagc
     9661 gagatcgagc acgcgttgtg gttcgagaag actgtaagga tgggctggtg cctgctgacg
     9721 caaagattcc agtccactgt gaatgtctgt caccggttga ccatcaggca gatggctcag
     9781 caaatccagc gaaccggatg agaaatgcag cgcgctttga ccacgcgtga caatggcaca
     9841 gcgcaggccg tgtttttgca gttgcaggcc acagagtaat ccggcgaggc cgccgcccat
     9901 aatgacagta tcaaagcgca tctttctgct ccttctccag accacacagt ccctgataaa
     9961 cccagcgggt aaattcgctt tcgcgcagtg cgtctcccca ggcgatgggt tgcacgcctt
    10021 tccagcgttc gttaaggaag gtggaaagtt gctcgataga ttgcgcggac gtcgtgacgt
    10081 taaaccgttg cagcagtccg gcagcgcggc aggcgcagag ttcgccctgg caggtgccca
    10141 tccccacacg ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa ttttctaccg
    10201 cgtactgcac ttcacccgca gttaccgctt cgcactcaca taccaggcta cggtgcagac
    10261 ggccttcgct cagccaggca ggcgtgcgat cgccatgacg ataaaccgca gaaccgcgca
    10321 gcggggcagg cagggagatg actttacgca aggtaacttc agcgggttct tgtgaaccag
    10381 gcagtgccag atcggcagtc gtacaggggc gcgtgttgcc cagtttgcgg cataccgcgt
    10441 cggtagccca ttcagccatc agccgatagg tcatcagttt gccaccggtg atggtgataa
    10501 atccgtccag accatcgcgt tcagcatggt cgagcagcac gatgccacgg ctgacgttac
    10561 gtccgctcgg gtcgtcatcg ctggcaacca gcgggcgcac gccagaatag gcccgcaaaa
    10621 tgcgcgtttt cgccatcacg ggggccagtt tttccccttc acgcagcaga atatcaacct
    10681 cttctgccgt cactcgatta tcgtcaatct cgttgtaatc aatacgtaaa gaggtggtac
    10741 caatcagcga aatggtatcg ccaggcacca gaatatcggc gtcggaaggt ttacggcagc
    10801 ggttgatcac atgctggtta atgcggtgat ccatgatcag cagcgatcct ttcgccggga
    10861 acatgcgaat gcgcagatcg gcatattcgg caatgtgttg cccccagatc ccagcggcat
    10921 taaccacgac aggtgcatga agggcctgag tttcgccggt gagatggtta cgtacacgaa
    10981 caccgcacac cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg gtaaggataa
    11041 cggcaccgtg ttctttggca tccagcatgt ttgctgcggt cagacgaaat ggatcaacgg
    11101 tgccatccgg aactttcacc gcgccaatca gtgccgggtt aacggcaggt tcgataatgc
    11161 gcgcttgctg cgggtctata gcttctgcgc tgatccctgc ttcttcgcag gcgcgaataa
    11221 aagtggcctg gaatgagagg tcatcttccg gcagggtgat aaacaggccg ttggttggtt
    11281 caacgcagtg acgtgcaatg cgtttcagga tctggttttc actaatgcat tcgcgggccg
    11341 attccgcatc ggttaccgca tagcgcgcac cgctgtgcag caggccgtgg ttacgcccgg
    11401 tggcaccggt tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg cgcagggcac
    11461 agtcgcgggc aatcccggct cccgttgcgc cgccaccaat gataatcacg tcacttgatt
    11521 gcgagtcgcg agttttcatt gttatccctc tgaagttcgt tttttaccat ttagccatag
    11581 taaaaacatg aattgtttga tttcgcgcat attcgctcat aattcgaaag tgaaacgtga
    11641 tttcatgcgt cattctgaac attgtgtaaa tcttatttaa taatgtgtgc ggcaattcac
    11701 atttaatttc tgaatgtttt cttaacatcg cggcaactaa agaaacggca ggttctccca
    11761 ctgaatcagt ctgttaatca taaataagac cacgggccac ggaggctttc aatgttgagt
    11821 atttttaaac cagcgccaca caaagcgcgc ttacctgctg cggagatcga tccgacttat
    11881 cgtcgattgc gctggcaaat tttcctgggg atattctttg gctatgcggc ttactatttg
    11941 gttcgtaaga actttgcgct tgctatgcct tatctggtcg agcagggatt ctctcgcggt
    12001 gatttaggtt tcgcgctttc ggggatctcg atcgcttatg gattttcgaa attcataatg
    12061 ggttcggtat cagatcgctc gaatccgcgc gttttcctcc ccgcaggttt gattctggcg
    12121 gcggcagtga tgttgtttat gggctttgtg ccatgggcga cgtcgagcat tgcggtgatg
    12181 tttgtacttt tgttcctctg cggttggttc caggggatgg ggtggccgcc gtgtggtcgt
    12241 actatggtgc actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt gtggaactgt
    12301 gcgcacaacg ttggtggtgg tattccgccg ctgctgttcc tgctggggat ggcctggttc
    12361 aatgactggc acgcggcgct ctatatgccc gctttctgcg ccattctggt ggcattattc
    12421 gcctttgcga tgatgcgcga taccccgcaa tcctgtggct tgccgccgat cgaagagtac
    12481 aaaaatgatt atccggacga ctataacgaa aaagcggaac aggagctgac ggcgaagcaa
    12541 atcttcatgc agtacgtact gccgaacaaa ctgctgtggt atatcgccat cgccaacgtg
    12601 ttcgtttatc tgctgcgtta cggcatcctc gactggtcac cgacttatct gaaagaggtt
    12661 aagcatttcg cgctggataa atcctcctgg gcctacttcc tttatgaata tgcaggtatt
    12721 ccgggcactc tgctgtgcgg ctggatgtcg gataaagtct tccgtggcaa ccgtggggca
    12781 accggcgttt tctttatgac actggtgacc atcgcgacta tcgtttactg gatgaacccg
    12841 gcaggtaacc caaccgtcga tatgatttgt atgattgtta tcggcttcct gatctacggt
    12901 cctgtgatgc tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc ggcaggtacg
    12961 gcagcgggct ttaccgggct gtttggttac ctgggcggtt cggtggcggc gagcgcgatt
    13021 gttggctaca ccgtggactt cttcggctgg gatggcggct ttatggtaat gattggcggc
    13081 agcattctgg cggttatctt gttgattgtt gtgatgattg gcgaaaaacg tcgccatgaa
    13141 caattactgc aaaaacgcaa cggaggctaa tggcatgaaa ttgacgctga aaaaccttag
    13201 catggcgatc atgatgagca ctatagtcat gggaagcagt gcaatggcgg cggacagcaa
    13261 cgaaaaaata gtcatcgccc atcgcggtgc cagtggatat ttgccggagc atacgctgcc
    13321 agcaaaagcg atggcgtatg cgcagggagc ggattatctg gaacaggatt tggtgatgac
    13381 caaagacgac catctggttg ttctgcatga ccattacctc gatcgtgtta ctgatgttgc
    13441 cgatcgtttc ccggatcggg cgcgcaaaga cggtcgttac tacgcgatag atttcacgct
    13501 ggatgaaatt aagtcgttga aatttaccga aggtttcgat attgaaaacg gtaaaaaagt
    13561 gcagacttat ccggggcgtt tcccaatggg taagtccgac ttccgggtgc acacctttga
    13621 agaagagatt gaatttgttc aggggttaaa tcactctacc gggaaaaata tcggtattta
    13681 tccagaaatc aaagcgccgt ggttccatca tcaggaaggg aaggatattg cggcaaaaac
    13741 gctggaagtg ctgaagaaat atggttacac cggtaaagac gataaagttt atttgcaatg
    13801 ttttgatgct gatgagctga agcgtattaa gaatgagctg gaacccaaaa tgggcatgga
    13861 gctcaatctg gtacagctga ttgcctatac cgactggaat gaaacgcagc agaaacagcc
    13921 ggatggaagc tgggttaatt acaactacga ctggatgttt aagccgggtg ccatgaaaca
    13981 ggtggcggaa tatgcagatg gtattggtcc ggattaccat atgttgattg aggagacatc
    14041 gcagccgggt aatatcaaac tcactggcat ggtgcaagat gctcagcaga ataaactggt
    14101 agtgcatcct tataccgtgc ggtcagataa actgcctgaa tacactcctg atgtgaatca
    14161 gttatatgat gctctgtata agaaagcggg tgtaaatggg ctgtttactg atttccctga
    14221 taaggcagta aaatttctta ataaagagta atgttactgc aggatggcgt tcatgcgcca
    14281 tcctgtctca tatcaaattt ttccaatcgc cactaattgt tccatgcaca tttgtagtaa
    14341 aaacagtgga ctgccttcta ccggcttttc tttagccagc aacgaaatct gtcgatagaa
    14401 agttggttcg agaggaacgg aacacaattc acctgcaatg cttttcagcg ttaactctgg
    14461 ttgtaaggcg atgcctaatc cctgtcgaat aaaattcatc gctgtatccg cctgattaaa
    14521 ttcacatttt atctgctggg cgcactatta gtggtagtgg ctggtggaat ctatgcgctg
    14581 caaatgaagg gtaaaagcgg tgtcacgcta gtagtggcaa aagaaattca ttaatacgac
    14641 acgtttcatt aagattttcc tcaggtaacc acctatagtc attgtcgtta ataaaaatga
    14701 caggcgatag ggtatggcag tttcagcaaa gtacgacgaa tttaatcact ggtgggcaac
    14761 agagggcgac tgggttgaag agcctaacta tcgccgtaat ggtatgagcg gggtgcaatg
    14821 cgtcgagcgc aacggcaaaa agctgtatgt aaagcgcatg acgcatcatc tgtttcattc
    14881 cgtacgttat ccgttcggcc gaccaacgat tgtccgtgag gttgcggtaa ttaaagaact
    14941 ggaacgggcc ggtgtcatcg tgccgaagat cgtttttggt gaagcggtga aaattgaggg
    15001 tgaatggcga gcgttgctgt tgactgaaga tatggcgggg ttcatcagca ttgctgactg
    15061 gtatgcccag catgcagtat cgccttattc tgacgaagta cggcaagcca tgttgaaagc
    15121 agtggcgctg gcgtttaaga aaatgcatag cattaatcgt cagcatggct gttgttatgt
    15181 tcgccatatc tatgtgaaaa cagaaggtaa tgcagaagct ggttttcttg acctggagaa
    15241 aagtcgtcgc cgcttgcgtc gcgataaagc gattaatcat gacttccgcc agctggaaaa
    15301 atatctggag ccaatcccca aagcggactg ggagcaggtg aaagcgtatt actacgccat
    15361 gtaatcgttg taggccagat aagacgcata agcgttgtat ctggcactac aaccaattca
    15421 catctcgatt tcaatatcgc cttttgcccg gcaacaacag ggcaaaattt cccccggctg
    15481 aataaaggct aacggttcgg caatccagtc aacctgacct gcaaccagac gggtgcgaca
    15541 ggagccgcag taaccttcgc gacactgata ctcaaccgcc acattgaggg attccagcgc
    15601 cgccagaagg gaagggtgtt catcctggca cagcagttgt gtgccagtga tgcgcagggt
    15661 aacgcgggcc atcagagttg gaagttactc aaatcgtcgg tgtccacttc cgcgtcaatc
    15721 tgcccgacca gataagaact gacttccact tcctgcggag caacctgcac gttatcagac
    15781 accagccagg tgttgatcca tgggatcggg ttggagcgcg tctggaacgg cagatccaaa
    15841 ccgactgcct gcatacggat attggtgatg tattcaacgt actggcagag aatgtcttta
    15901 ttcagaccaa tcatcgaacc gtcgcggaac agataatccg cccagtcttt ctcctgttga
    15961 gctgcctgaa caaacaggtc atagcactcc tgcttacact cttcggcaat ttccgccatc
    16021 tcaggatcgt ccgcgccgct gcgcagcaga ttcagcatat gctgggtgcc ggtcaggtgc
    16081 agggcttcgt cgcgggcaat caggcgaata attttggcgt tgccttccat caattcgcgt
    16141 tctgcaaatg cgaaggaaca agcaaagctg acgtagaaac gaatcgcttc cagcgcgtta
    16201 acgctcatca ggcagagata cagttttttc ttcagctcgc gcaggctaac ggtcacagtt
    16261 ttaccgttaa cggtgtgggt accttcgccc agcagatgcc agtagctggt catttcgatc
    16321 agctcatcgt aatagctgga gatcccttcc gcacgtttct ggatctgctc gttggtgacg
    16381 atatcgtcaa acacaacaga cggatcgtta acgatattac ggatgatatg agtataggaa
    16441 cgggaatgaa tcgtttctga aaacgcccag gtttcgaccc aggtctccag ttccgggatc
    16501 gagatcagcg gcagcagtgc cacgttcggg ctacggccct gaatggaatc cagcagtgtc
    16561 tgatatttca ggttgctgat aaagatgtgt ttttcgtgtt caggcagtgc ctggtaatcg
    16621 atacggtcgc gagaaacgtc aacttcttcc ggacgccaga agaaagagag ctgcttttcg
    16681 atcagctttt cgaagatgtc atatttttgc tgatcgtagc gagccacgtt gaccggctga
    16741 ccaaagaaca tcggttcttt gagctgatca tttttcgtct gtgaaaaggt ggtatatgcc
    16801 atgagtgtgt cctgttggga gtttaatgcc ggataaggca ttttacgccg catcctgtgc
    16861 ctgatgcgac gctggcgcgt cttatcaggc ctacaaatcc gagccgtagg ccggataagg
    16921 cgttcacgct gcatccggca cccggagcct gatgcgacgc tggcgcgtct tatcaggcct
    16981 acaaaccgag ccgtaggccg gataaggcgt ttacgccgca tccggcatct caatatcaga
    17041 tcttacatgc gccgctttcg cagccatcgt cctggattga cggcaccaga tcgtcctgcg
    17101 cgtcttcagc gccgtcacgg gtgttctgat aatacagcgt tttgaccccg aatttgtagg
    17161 cggtgagcag gtctttcagc aactgctgca tcggcacttt tcctgacggg aaacgtgacg
    17221 gatcgtagtt ggtgttagca gagatcgact gatcgataaa tttctgcatg atacctacca
    17281 gttgcaggta gccatcgtta cccggcattt cccacagcag ctcataggcg tcgtgcaggt
    17341 gctcgtagtc cggcaccacc tggcgcaaaa taccgtcttt cgacgctttg atgctgacgt
    17401 aaccgcgcgg cggttcaata ccgttagtgg cgttagagat ctgcgaagag gtctcggacg
    17461 gcatcagggc agaaagcgtg gagttacgca ggccgtgcgt tttgattgat tcacgcaatg
    17521 cttcccagtc gtaatgcagc ggctcattag cgatggcatc cagatctttc ttataggtat
    17581 cgatcggcag gatccctttc gcgtaagtgg tttcgttaaa ccacgggcac gcgccttgct
    17641 ctttagccag ctcattagac gctttcagca ggtaatactg aatcgcttcg aaggttttat
    17701 gcgtcaggtt gttggcgctg ccgtcggagt agcgtttacc gtgcttcgcc agatagtaag
    17761 cgaagttaat cacaccaata cccagcgtac gacgccccat cgctccacgt ttggcggccg
    17821 ggatcgggta atcctgataa tccagcagcg cgtcaagtgc acgaaccgcc agaattgcca
    17881 gctcttccag ttcatccagg ctattaattg cgcccaggtt gaaagcagac agcgtacaca
    17941 gcgcgatttc accgttctcg tcgttgacgt cgttcagcgg tttggtcggc agggctatct
    18001 ccaggcacag gttagactga cgcactggcg cgatggccgg atcaaacggg ctatgggtat
    18061 tgcagtggtc aacgttctga atatagatac gaccggtaga cgcacgttcc tgcatcatca
    18121 gcgagaacag ctcaacggct ttcacacgct gcttgcggat gctgtcgtct ttctcatatt
    18181 tggtatacag gcgttcaaac tcttcctgat cggcgaagaa tgcgtcgtac agccccggta
    18241 cgtcggacgg gctgaacagg gtgatatctt cacctttcag caggcgggta tacatcagtt
    18301 tgttgatctg taccccgtag tccatatggc gcacgcggtt gccttccaca ccacggttgt
    18361 ttttcaacac cagcaggctt tccacttcca gatgccacat cgggtagaac agcgttgccg
    18421 caccgccgcg cacgccgccc tgagagcagg atttcaccgc tgtctggaaa tgtttgtaga
    18481 acggaataca gccggtatgg aacgcttcac caccgcgaat cgggctaccc agcgcacgaa
    18541 tacgcccggc gttgatgccg atcccggcac gctgggaaac gtatttcaca atcgcgctgg
    18601 aggtagcgtt gatggaatcc agactgtcac cgcactcgat cagtacgcag gagctgaact
    18661 gacgagtcgg ggtacgcacg ccggacatga ttggcgtcgg cagcgaaatt ttaaatgtgg
    18721 aaaccgcgtc gtaaaaacgc ttcacatatt gcaggcgcgt ttcacgcggg tagttcgaga
    18781 acaggcacgc ggcaaccaga atataaagga actgggcgct ttcgtagatt tcgccggtca
    18841 cgcggttctg taccagatat ttgccttcca gctgcttaac ggcagcataa gagaaggtca
    18901 tatcacggtc gtgatcgata aacgtgtcca tctgcttgaa ctcttcttcc gtgtagtctt
    18961 ccagcagatg attatcgtat ttgcccatct cgaccatttt caccacgtgg tcgtacagcg
    19021 caggcggctc aaactggccg taggcttttt tacgcaggtg gaagatcgcc aggcgcgcgg
    19081 cgagatactg ataatccggc gcatcacggg agatcaggtc tgcggcagcc ttgataatgg
    19141 tttcgtggat gtcagaggtc ttgataccgt cataaaactg aatgtgggag cgcagctcga
    19201 cctgggaaat cgaaacgtta tgcagtcctt ctgccgccca atccagaacg cgatggattt
    19261 tgtcgagatt gatgcgctct gtgctaccgt cgcgctttgt caccagcaga ttctgattca
    19321 tgtatgtcgt acctgttttt ggaattcttt ccgcaatagt ttcatgcaga ctacctgtag
    19381 tacgggaagc tgcttagaac actatatata gggggtatgt ttgggattca ctgcaagata
    19441 gtgtgaaaat ggccctcttg caagtgcata actttgtgga taactcagga aggaaaaagt
    19501 ggctttcgcg caccttaggt cagacaaggt gtccgggaaa gtcaatggga agagaaaaat
    19561 ttgttaaaaa taactgttcg gcgatttctt gaacggcaga ataggttgga taaattgata
    19621 tagatggttt attaatcttt ttgctaatga ttgcaaaaat tgatctaagc acaagccatc
    19681 acataatctg atgtatcagt tgtggtcaat atataagcga atgtaagcga ttgttgatgt
    19741 cgaaatggtt gttttctatt cggttattgt taaatgcgaa gccattttgt taatttacct
    19801 tatattataa gaaaattatt cattacttta
//
LOCUS       DAJSKJ010000063        19547 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000063 DAJSKJ010000000
VERSION     DAJSKJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.063"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..314)
                     /locus_tag="ON345_001957"
     CDS             complement(<1..314)
                     /locus_tag="ON345_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3072019.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLL"
     gene            complement(513..1415)
                     /locus_tag="ON345_001958"
     CDS             complement(513..1415)
                     /locus_tag="ON345_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR3072020.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQLWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1553..2794)
                     /gene="yihS"
                     /locus_tag="ON345_001959"
     CDS             complement(1553..2794)
                     /gene="yihS"
                     /locus_tag="ON345_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR3072021.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2811..3689)
                     /gene="yihT"
                     /locus_tag="ON345_001960"
     CDS             complement(2811..3689)
                     /gene="yihT"
                     /locus_tag="ON345_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR3072022.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3713..4609)
                     /gene="yihU"
                     /locus_tag="ON345_001961"
     CDS             complement(3713..4609)
                     /gene="yihU"
                     /locus_tag="ON345_001961"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR3072023.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4777..5673
                     /gene="yihV"
                     /locus_tag="ON345_001962"
                     /pseudo
     CDS             4777..5673
                     /gene="yihV"
                     /locus_tag="ON345_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
     gene            5707..6492
                     /locus_tag="ON345_001963"
     CDS             5707..6492
                     /locus_tag="ON345_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3072024.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6591..7190
                     /gene="yihX"
                     /locus_tag="ON345_001964"
     CDS             6591..7190
                     /gene="yihX"
                     /locus_tag="ON345_001964"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR3072025.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            7184..8056
                     /locus_tag="ON345_001965"
     CDS             7184..8056
                     /locus_tag="ON345_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR3072026.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            8053..8490
                     /gene="dtd"
                     /locus_tag="ON345_001966"
     CDS             8053..8490
                     /gene="dtd"
                     /locus_tag="ON345_001966"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR3072027.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8535..9476
                     /gene="fabY"
                     /locus_tag="ON345_001967"
                     /gene_synonym="yiiD"
     CDS             8535..9476
                     /gene="fabY"
                     /locus_tag="ON345_001967"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR3072028.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9540..10448)
                     /locus_tag="ON345_001968"
     CDS             complement(9540..10448)
                     /locus_tag="ON345_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3072029.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10677..10988
                     /locus_tag="ON345_001969"
     CDS             10677..10988
                     /locus_tag="ON345_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3072030.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10989..11279
                     /locus_tag="ON345_001970"
     CDS             10989..11279
                     /locus_tag="ON345_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3072031.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            11884..12102
                     /locus_tag="ON345_001971"
     CDS             11884..12102
                     /locus_tag="ON345_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR3072032.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            12321..12563
                     /locus_tag="ON345_001972"
     CDS             12321..12563
                     /locus_tag="ON345_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072033.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(12893..13822)
                     /gene="fdhE"
                     /locus_tag="ON345_001973"
     CDS             complement(12893..13822)
                     /gene="fdhE"
                     /locus_tag="ON345_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR3072034.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(13819..14454)
                     /gene="fdoI"
                     /locus_tag="ON345_001974"
     CDS             complement(13819..14454)
                     /gene="fdoI"
                     /locus_tag="ON345_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR3072035.1"
                     /translation="MKQRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(14451..15353)
                     /gene="fdoH"
                     /locus_tag="ON345_001975"
     CDS             complement(14451..15353)
                     /gene="fdoH"
                     /locus_tag="ON345_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR3072036.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(15366..18416)
                     /gene="fdnG"
                     /locus_tag="ON345_001976"
     CDS             complement(15366..18416)
                     /gene="fdnG"
                     /locus_tag="ON345_001976"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(17829..17831),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR3072037.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            18610..19443
                     /gene="fdhD"
                     /locus_tag="ON345_001977"
     CDS             18610..19443
                     /gene="fdhD"
                     /locus_tag="ON345_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR3072038.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELCRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         4648 a         4903 c         5159 g         4837 t
ORIGIN      
        1 agcaataaac gcccctgatc gtcggcagag atattcagcg tggcgctaat gtcagaacca
       61 cggctgaaat gaattaacca accatccggt gactggctga cgatggcgtc ggtaagcgca
      121 attttctcct gtagtttatc tttaatgctg aaattaccgc ggaacatatc gatatccgct
      181 atacctgagc caatccataa acaaggatta tctttgctat gggttaaaat aagacgttgt
      241 tgaaaatgta gggtaaaact gtcgttattc tgatgaaatt gaaaatctaa taactgtgga
      301 cgtggcgtat ccataactaa tctccataga gttgagaacg agattgttgc cttcctgtta
      361 ttacaggctt atgtaaaatg attctcataa aactgatata taaaaacttt acagaagctg
      421 agctgttttt aacttaataa attgaagata cataatgttg cctttagcga gtgaaatgtt
      481 cttcaatgat tttgactgct aatcaataca agttattttc tctgtccgta aatattaaaa
      541 aacaggcggt gcggttttcc tggttccagt aataataagt cgatacctga attaaaggcg
      601 ttaggcgggc aactcatagg ttcaactgcc agtccctgac gctgtaattt ttcaccgcta
      661 taaacctgca cccagagttg atcggaacaa aggcttaccg aaagcgcctg ctgtggatgg
      721 gtaatggtca tttcccacaa atcattagcg gttttaaagg tgtggtcgat ttttgtcgcg
      781 gctatttttt tcgcctgcgt gaaatttaaa tccagctcat caacgtggtg aagcgttgtc
      841 ggattcgcgt gctcatcgac ggcataaacc tggtttgccg gaagctggaa cagatattca
      901 tcaacagatg ttaagttgca ggttaaataa ggatgaatac ccacgccata aggcgctgcg
      961 acagtgccga tattctggct tgcgatctca acggaaagac ccgtgtgtgc gttgagcgaa
     1021 taaacaactt gtgaagccag cataaacgga tagccgtaac tgggcggcag aaaagctgtc
     1081 agggtaacgc tggtggcggt taattcacta atttgccagt ctcgccaggc aagcagcccg
     1141 tgaatagcgg cttttgaact atgttcatta atgggtaact gatattcctg cccctgatag
     1201 cggtaacagc cgttggcgat ccggttcggc cacggaataa gcactttacc cagatgcgcc
     1261 agcggcatct cttcgggttt gtgcgggatg actaaatgac aaccctgaaa ggttaattca
     1321 gccagaccag caccgacggt cacaatggtc gcgtgataat cccccgcggc aagtgaaacg
     1381 gtctgtccac tgcaatgcat attggtgatt tgcatacaag gcaccttaat taacgacata
     1441 cagcctcccg tgaggctgat gtcaaaccca aaaactgcac cggacggtcc cctcgcccct
     1501 ttggggagag ggttagggtg aggggaaaac ccgcacccta ttttcagatc agttatttcg
     1561 cattaatatc cagcagaccc gctgcaaccg ctggagccat gccgggcgct aacgggatac
     1621 gcgggatcac caggcaatgc agcaggtgat aaatatcctg tttgccgtcc cagactttgg
     1681 tggtgacctt attgtccgca tccagctcct gccaccagga accattttca tagtccatca
     1741 ggtacttaat gcagtactcc caccatgttt gataccaggt ttcatactgg cgatcgccag
     1801 tgacggtgta gagcgcgtag gccgtaccca ttgcttcgac gataggccaa cgtacacgtt
     1861 cgcggaccac cggttttcct tcccagtcaa cggtataaac aatcccgtcc gcgccatcgg
     1921 gtgcccaggc atcgcgcacg gtggcgttaa acagaccttt ggcatcttct agcagccatg
     1981 ctggtggttg ttcgcaacgg gcttccaggg cagcatggat gtgcagcatt aaacggcccc
     2041 attcgatcca gtggcctggt gtacctccga acgcgcggaa gcgatgcgcc gggttatctt
     2101 tgttgtaatc cggcagcgga ttccactggg tatcgaagtg ttcgttaacg cgataatgat
     2161 tatttctggc gacgtcgtgg ataatcacgg aagccacgcg aatcgcgcga tccagccatt
     2221 ttttgtcgtg agtgacgtca taaacaatca agaaagcttc caccgcgtgc atattggcat
     2281 tgccgccgcg gtactcttcg gttttgctga aggcttcgtc ccaggattcc aggcacatct
     2341 gctcttcttc gctccagaaa tatttctcga taatttcaat ggtgtaatcg agcagcttgc
     2401 gcgcttccgg gtgacccgtt gtgacggcgc tggcggcacc cagcagagca aagaagtgtt
     2461 gatagccctg tttggaggca tccaccacgc cctcgtcatt cacgcaggca taccagccgc
     2521 catatttttt atcacgcagt gcgccgttca tggctttgat accgtgatca accaacgagt
     2581 aagcgccagg tcgacccatc gccgcagcaa cggaataaac gtgtaacata cgagctgtga
     2641 tccacagatg ggtgcccatc tcttctttga tttgcccttt attgcctaac cagccaaaac
     2701 cagtcggcac tacggaattt ttaccaaaat caaagatgcg gtcggtttcc tgttccagcc
     2761 aacggttgtg gcttagggtg ttaaaccatt tcattccttt tatcctcatc ttagcggcgt
     2821 ttagccatca tttcgtcgac gatatcgcca agttgttgta atttcggtgc gcaaacgtca
     2881 cgcagcatca gctcgttgtc tggtaaaccg acgaccgatg cccagactgc acggcctgcc
     2941 aggaatcccg atgcgcccgc cgtcattgcc acgcgtacgg cacgcgggaa cagtttttcg
     3001 tcgacaccgg aagagaggat cacccatggc atattgatat ggtcattcag acgttgtgaa
     3061 gcacagagaa gctcttgttg cggacctttg ccataaagcg gcatttcaac tttgtagagg
     3121 tcagcgccac tgtcgcccag ctctttggcg gcatcgatga tcgcttgttc gcgatcgaat
     3181 ttatcgccac gacgcggtgg gcggacgact ggctcaatga tgcttaccag accgtgtgag
     3241 tggcacagtt cgttgaactc tttcaccata tccagacgtt gctgcgcatc ttcatcacta
     3301 cgccacagca ccagcagttt taaggctttg ccaccgtctt gtttgatctg tagcggattg
     3361 attttgcggt caataaccac gctatcgacc ggaataccgt tgccaggaat gaactcatcg
     3421 gcggcgacaa tcatggcgca acttttggca atcgcgtttt gctcaaccac ctggcggtag
     3481 cagaattgtt gatctaccag aatcgccgag gcataaggtg agagggcctt tgcagcgtta
     3541 actttgaaat cagttaaaac gctatcggct accggagcgg gtgccccagc cgcggcaaac
     3601 atcatgcgca tggcttcgcg ctgatcgacc gccagcatgg caaaaccgcc cgatgcgcgc
     3661 gtaatgtcgt tgatggtgta cttattcatt catttattcc tttatccagt cgttacattt
     3721 ttactttggc agtcatcccg gcactgacac ggacctgttc cagaatggcg gaccagtcct
     3781 ggcgaccgcg acccgctgcg cgcgcctggc tataaacctc ccgtgaggcg gcccccaacg
     3841 gcattggcac atgcagctgg ttggcgacat caagggcgat gccaagatcc ttatgggcaa
     3901 gatcgatcat gaaggcggga gaaagatctc cgctgaggac tttgtttggc caggaagtgg
     3961 tgaagtgacc tttaccggcg gcggtaccgc tcatcacttt gacggcaaca tcgaagggaa
     4021 gattcagggc ttcgcacaaa acggcggctt ctgccgaaag cgcattgagc gcgatgctca
     4081 tgtagttgtt gatgagctta acgcggatcc ccatgcccgg accgccagcg ttgatcaact
     4141 cactgcccat cgccatcagg atcggtgtgg cacgttcaac ttgttcagcg gtgccgccag
     4201 ccagcagtaa cagagtaccg gtaatggcat ttgcagaagt acggcctacc ggaacatcca
     4261 tcatgctgaa gcctttggct tgcatatcgg caatcaattt atcggtttgc agcggatgga
     4321 tggtggacat atcaatgacc agcgcatcgg tagataagcc ttcgcaaacg ccgttttcac
     4381 cgaacaacac gttgcgcacc agatcgccat tcggcaacat ggtaatgata aattcggcat
     4441 ctttagcggc ctgcgccggg ttggcggcgg gagtcgcacc tttgtctacc agatgccgca
     4501 cagcttcggc gttcacatca aagacgcgaa gttgatgccc ttgctgcaat aaattgctcg
     4561 ccattggcga acccatttgt cctaaaccga taaacgcgat tgctgccata accctctcct
     4621 gaatacagtt atgtcacttt ttgtcattta tgacatgcct tgcttgtctt tttttgatcg
     4681 tatttgtaat ttatcgtcaa aaaattgaca gccgtcactt tttaaacaat tggtgaaatt
     4741 aaaatgaacg catcccaaaa tgtttaagga atgaccatga ttcgtgttgc ttgtgtaggt
     4801 ataaccgtga tggatcgcat ctattacgtg gaagggttac cgacggagag cggtaaatac
     4861 gtggcgagaa attatacgga agttggtggc gggccagcgg cgacggcagc ggttgcggcg
     4921 gcaaggctgg gggcgcaggt cgattttatt ggtcgcgtag gtgatgacga cacgggcaac
     4981 agcctgctgg cagagctgga atcctggggc gttaacaccc gttacaccaa acgttataac
     5041 caggcgaaat cttcgcaatc cgccatcatg gtggatacca aaggcgagcg gataatcatt
     5101 aactacccca gcccggatct gctgcctgac gcagagtggt tggaggaaat tgatttctct
     5161 cagtgagatg ttgtgctggc agatgtacgc tggcacgacg gcgctaaaaa agccttcacc
     5221 ctggcccgtc aggcgggtgt gatgaccgtt ctggacgggg acattacgcc gcaggatatc
     5281 agtgagctgg tggcattaag cgatcacgcg gccttttcag aaccgggtct ggcgcgctta
     5341 acgggcgtga aagagatggc cagtgcgcta aaacaggcac aaacgctcac aaatggtcat
     5401 gtctatgtga cccagggtag cgcaggctgc gactggctgg aaaatggtgg gcgtcagcat
     5461 caaccggcct tcaaagttga tgtggtagat accacaggtg cgggtgatgt ttttcacggc
     5521 gctttggcgg tggcgctggc aacaagtggg gatttagcgg agtcagtccg cttcgccagc
     5581 ggtgtagcgg cgttaaaatg cacacgtccc ggtggacgag ccgggatccc tgactgtgat
     5641 caaacccgat cttttttgtc actttttgta taaaatgcca gggtgatggt ttttcgagga
     5701 attttcatga gccttaccga actaaccggt aacccgcggc acgaccaact cctcatgctg
     5761 atcgccgagc gtgggtatat gaatattgat gagctggcaa atctgctgga tgtctccacg
     5821 cagacggtcc gccgggatat tcgtaaatta agcgagcaag gcctgattac gcgccatcac
     5881 ggtggcgcgg gtcgggcttc cagcgtcgtt aatacggcgt tcgagcagcg tgaggtttcg
     5941 caaaccgagg aaaaaaaagc gattgccgaa gcggtggcag actatattcc tgatggttca
     6001 acaatattta tcaccattgg tacgactgtt gagcatgttg cccgggcgtt acttaaccat
     6061 aatcatttgc ggataatcac caacagcctg cgtgtggcgc atattcttta ccacaacccg
     6121 cgctttgaag tgatggtgcc cggcggtacg ttgcgctctc ataatagcgg gatcattggc
     6181 ccttcagcgg cgtcctttgt ggctgatttt cgtgctgatt atctggtaac aagcgttggg
     6241 gcgattgaga gcgatggcgc gttgatggag tttgatgtaa acgaagctaa cgtggtgaaa
     6301 acgatgatgg cgcacgcgag aaatattctg ctggtcgccg atcacactaa gtatcatgct
     6361 tcggcggcgg ttgaaattgg taacgtggca caggtcactg cgctctttac cgacgagctg
     6421 ccgcccgctg cgctaaaatc acgcttacaa gacagccaaa ttgaaatcat tcttccccag
     6481 gaagacgcgt agatttttgt gaccgttaac ctggcttcat accttgccac atagccaaac
     6541 ccatcctttc ccgctacagt taatttcttg tggcgcgaaa ggaggcaaaa atgctctata
     6601 tctttgattt aggtaatgtg attgtcgata tcgactttaa ccgtgtgctg ggagcctgga
     6661 gcgatttaac gcgtgttcca ctggctacgc ttaagaagag tttccacatg ggggaggcgt
     6721 ttcatcagca tgagcgtggg gaaattagcg acgaagcgtt cgcagaggcg ctgtgtcatg
     6781 agatggccct gccgctaagc tacgagcagt tctctcacgg ctggcaggcg gtgtttgttg
     6841 cgctgcgccc ggaagtgatc gccatcatgc ataaactgcg tgagcagggg catcgcgtgg
     6901 tggtgctttc caataccaac cgcctgcata ccaccttctg gccggaagaa tacccggaaa
     6961 ttcgtgatgc tgctgaccat atctatctgt cgcaagatct ggggatgcgc aaacctgaag
     7021 cacgaattta ccagcatgtt ttgcaggcgg aaggtttttc acccagcgat acggtctttt
     7081 tcgacgataa cgccgataat atagaaggag ccaatcagct gggcattacc agtattctgg
     7141 tgaaagataa aaccaccatc ccggactatt tcgcgaaggt gttatgctaa aaaccattca
     7201 ggacaaagcc aggcaccgta cccgtccact atgggcctgg ctaaaactac tctggcaacg
     7261 cattgatgag gacaacatga caaccctggc aggtaacctt gcctatgtgt cgttgctctc
     7321 attagtgccg ctggttgccg ttgtttttgc gcttttcgcc gcttttccca tgttttccga
     7381 cgtcagcatt cagttacgtc actttatttt tgccaacttt ctgcctgcta ctggcgatgt
     7441 tattcagcgg tatatcgaac aatttgttgc caattccaac aagatgaccg ccgttggggc
     7501 gtgcgggctg atcgtcaccg cgttattgct gatgtactcc atcgatagcg cgttgaatac
     7561 catctggcgc agtaaacgag cgcgaccaaa aatttactcg ttcgccgtgt actggatgat
     7621 tttaacgctg gggccgctgc tagcaggggc cagtctggcg atcagttcct atttgctctc
     7681 cctgcgctgg gcgagcgatc tcaatactgt catcgacaac gtgctgcgta tttttccgtt
     7741 gctgttgtcg tggatctcct tctggttgct gtacagcatt gttcctacca tccgcgtacc
     7801 taaccgcgac gcgattgtcg gcgcgtttgt cgccgcgctc ctgttcgaag caggaaagaa
     7861 aggtttcgcg ctttatatca ccatgttccc gtcatatcag ctcatttacg gcgtgttggc
     7921 ggtgatcccc attctctttg tttgggtcta ctggacatgg tgtatcgtct tgcttggcgc
     7981 ggaaattact gtcactctcg gggaataccg caaactaaaa caagcagctg aacaagaaga
     8041 agacgacgaa ccatgattgc attaattcaa cgcgtaaccc gtgccagcgt caccgtggag
     8101 ggagaagtga cgggcgaaat tggcgcggga cttttggtgt tattgggtgt cgaaaaggat
     8161 gacgacgaac agaaagcaaa ccgtctgtgc gagcgtgtgc tcggctaccg catctttagc
     8221 gatgccgaag gcaagatgaa cctcaacgtg caacaggcgg gcggcagtgt gctggtggtt
     8281 tcccagttta ccctcgccgc agataccgaa cgggggatgc gcccaagttt ctccaaaggc
     8341 gcatcaccgg atcgcgcaga ggcgttatat gactatttcg tcgaacgctg ccgtcagcaa
     8401 gagatgaaca cgcaaacagg acgcttcgct gccgatatgc aggtatcgct ggtcaatgat
     8461 ggccccgtga cattctggtt gcaggtatga gccagcttcc agggttgtca cgggaaacaa
     8521 gagagagtat cgctatgtat caccttcggg ttccacaaac agaagaagag ttagagcgtt
     8581 actatcagtt tcgctgggaa atgttgcgta agcccctgca tcaaccaaaa ggttcggaac
     8641 gcgacgcgtg ggatgcgatg gcgcatcacc agatggtcgt cgacgagcag ggtaatctgg
     8701 tggcggtagg ccgattgtat attaatgccg acaatgaagc gtccattcgc tttatggccg
     8761 ttcacccgga cgtgcaggac aaagggttag gaacgctgat ggcgatgacc ctggagtcgg
     8821 tggcgcgtca ggaaggcgtt aagcgcgtga cctgtagcgc ccgtgaagac gcggtggagt
     8881 ttttcgccaa gctggggttt gttaatcagg gagaaatcac cacaccaacc accacgccga
     8941 ttcgccattt tttgatgatt aagcccgtcg ccactctgga tgatattttg catcgcggcg
     9001 actggtgcgc gcagctgcaa caggcgtggt acgaacacat cccgcttagt gaaaaaatgg
     9061 gcgtgcgcat tcagcaatat accgggcaaa aatttatcac caccatgccg gaaaccggta
     9121 atcagaatcc gcaccatacg ctgtttgccg ggagtttatt ctcgctggca acgctcaccg
     9181 gttgggggct tatctggctg atgctgcgcg aacgccacct cggcggaacg attattctgg
     9241 ccgatgcgca tatccgctac agcaagccga ttagcggtaa acctcatgcg gtagccgacc
     9301 ttggtgcctt aagcggcgat ctcgaccgtc tggcgcgcgg acgaaaagca cgggtgcaga
     9361 tgcaagttga aatctttggc gacgagacgc cgggtgcagt gtttgaaggc acgtatatcg
     9421 ttctgcccgc gaagccattt ggcccgtatg aagagggcgg gaacgaagaa gagtaggggc
     9481 gttttacttg actgaaaagg ttttcaatct gaaaaccttt tcagggccca ggtccgaagt
     9541 taccttcccg ctctccaggc aacatcccgt agtgccgatc gcgcggcctc gctgatacca
     9601 cctaactgaa aaaatccgtg gataacgcca aggtagcgtt ggcaggtgca ttgcactcct
     9661 tgccctgtca tgcggtgata caacgcttca ccttcatcgc aaagtgggtc gtattcagcg
     9721 gtaatgatat gtacaggtgg caagccattg aaatcatcgc gccaaagagg gctggcttca
     9781 ggatgcctgc gttcaatgcc tggcagatac atctcaaagc cgctcagtaa agtgtcgcgt
     9841 gtgatgatgt aatcgtgacc attgcgtatg tagctttcga aatgcgcagt cgcatcaagc
     9901 ataggataaa ttaagatgag ttgtgccggt tgccactcgc cagcacgctt taatcgcaaa
     9961 gcagttacca gcgccagatg tccgcccgca ctgtcaccgg caagagttat acgttgttta
    10021 tcaacgccga gtcgttcggc gtgttgccag actatttctg cacctcgttg cgcatcatca
    10081 tgggcggcgg ggaaagtatg ttccggtgcc agccgatact ggacggcaat aacccgacat
    10141 tgcccgtaat aggctaactg gcgcagctgg ttgtcgtgag ttgcgaaccc gccgctaaca
    10201 aaacagccac cgtagtaata aattatcgtc gggagtaatg tcggggcatt cagcggcgac
    10261 actattcgta aggtcatccc ttcaagttcg atagtctgaa tatctacccg cgtttctgtc
    10321 tcacccgcaa ggacagcgct ggcgatatag ccctccctgc gctgcgcaat actttgttga
    10381 cgcgatgagg ggcgacccgc agcgataaac ttttcgacta actctgcaat tcctttttcc
    10441 agtgccatag tagaacttat tttgctgtat gaatgtacag tcttatacac ccgtttttgc
    10501 tggatggata cagggaaatt cacgggcgtg aaaaatctgg aaagcgcctc gcaaatctaa
    10561 agacaaaccc ggcagagacg tggttttgct ggagcactat cagtaagcta gtggtttcaa
    10621 gggtacgtca atgacgtaca aaaagggttg ttactctaag ccaatggcgt ataattatgc
    10681 tcttcatcga aacggaaatt tttactgaag atgttcaaaa actgctgacc gacgatgaat
    10741 tcagtcgttt tcagtttttc ctggccttga atcctgacta tggtgaggta atacctgaaa
    10801 ctggtgggtt aagaaaggtt cgatgggtgt caggaggaaa ggggaaacgt gctggtgtca
    10861 gggttattta ctttcatcag gttaaacatt atgagatcag attactgctt atttatcgta
    10921 agggtattaa agatgatctg tctccccagg aaaaagcaat gctccggttg ttaaatacga
    10981 ggtggtaaat ggataaggca ctttttgagc ggttgactca cagcatggcc cagatgaatg
    11041 aaattattga aggaacgcga cagccttcgc ggacttttga agttgatgct atgaaaatca
    11101 aagaaatacg ccgcgcttca gggttgtcac aatctaaatt tgccgatctt atttctgtaa
    11161 gcgtagacac gttgcgtaac tgggagcagg ggcgacgttc accgaccggg cctgcaaagg
    11221 ctttgctgcg agcgattgcc aatgatccgc aacatgtgct gcaagcactt aaccgttaga
    11281 ggcatggtta tgccatacag aaagcactag cattcagaac tcctgtgaaa gcggcatagt
    11341 ggtcgcctgc ccaggagttt tgctatgacc cgtaatcgtg ctgaacgtcg ccaccctatg
    11401 atgcgattaa aaaaactcat cgtcgcgctg atgattgacg tcaatgctat cggatgatac
    11461 cggggggttc cggaaggaat tccttgtttc ccgaactgtt accaacaaaa ttataacgat
    11521 gaacaagtaa agaccggttg gtggcagtac tgaccggtct gtgatggatg attaatttca
    11581 ccttaaatcc gggtgctgtt aggatgctga catttttcgg gtacaccatt cagattatgc
    11641 aaataaagat ccaatcaaaa aataaacatc cctgaatata atatattcat agcatccata
    11701 actcatcctg ggcgataact aaaccatttt tgagtaataa atgaaacaac tgcaacgctc
    11761 ttattatttc tggaaaacgc ctcgcaaaaa caaaacgaca tgcaaaaatc cgcttttttt
    11821 aaatgatttt ccggcgtaat tttcgcacat acattaatga gtaatatatg taaggtggtg
    11881 gtaatggcga tgaatacggt ttttcttcat ttatcagaag aggcaattaa aaggctgaac
    11941 aaacttcggg gctggcgtaa ggtttcacga tctgcaattt tacgcgaagc ggttgagcaa
    12001 tatctggagc ggcagcaatt tccggtgcgt aaagcaaagg gaggcaggca aaagggggag
    12061 gtagttggtg ttgatgatca gtgtaaggag cataaggaat gaaaaaaatt tttcattctt
    12121 gaatataaaa aaccagatgc ccttattctg gtattaatac aaggctgttt tacttgaact
    12181 tataataact gcaactgtta catcgtatct ggaaaacgcc tcgcaaaaat aaaaaatgat
    12241 gcgtaaatga gctttcttta aagattcggc aagggtactt ttctctccat agattcaaaa
    12301 atgatattgg cgagatattt atgaactctc tggcaggtat agatatgggc agaattttac
    12361 tcgatttatc gaatgaggtg attaagcaac ttgatgatct cgaggtgcag cgtaatcttc
    12421 ctcgtgcgga cctattaagg gaagcggtag atcaatacct gataaatcaa tcgcaaacag
    12481 caagaaccag tgttcctggc atctggcaag ggtgtgagga agatggtgtc gaatatcagc
    12541 gtaagctgcg cgaggaatgg taatgggaaa aaatgatgtc aatcagattg ctgacaacgt
    12601 gcgcgttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa gtcatgcaaa
    12661 ttcaatatat tgaagtttat tggtaggcct gataagcgca gcgcatcagg caatcatacg
    12721 cttgtcatca gtgacatacc cataacctta tcgatgtgtt ggagggaaga acgaggcaaa
    12781 cagacaattg cggcatgatc ttgaatgggt aacgcggaat acgagagata gtgtcgtcac
    12841 accctatgag ctgtaatccg ggcgaccagc gccacccgga aaacgtcaga gattactccc
    12901 cttcacccgg aaacaggaac gggttaatgg aactgcgggc gtagccttct tgctccattc
    12961 gcgcgtccag taccagagag gcgaggtcat ctgccacggc ttcaactttc ggttcttttt
    13021 cctgataaag aatcttcagg taagtgccac agtcatcgca gctttcggct ttaatcgcgg
    13081 cctgttcgtc atccagcgac cagtaatgca gtttgccgct ctgctcgcag ttgctgcatt
    13141 ttacgcgcac tacgtgccat tcggtttcac acaggttgca gtgcaggtaa cgcagaccct
    13201 gagtggtgcc aatttgcacc atgctggaca ccggcataga accacaaacc gggcaatatt
    13261 gacgttgctc gccgtattca gcgcgggctt tgccggggat cagattggcc atctgcgccc
    13321 agtagagcga cagtgcagcc cagataaacg gcgctttatc gctgctgacg gatgagaaat
    13381 cagaggcaaa cagtgcgctg gccatatctt ccagctcctg agtcgatgcc ttctccagat
    13441 tctcaatcac tgccagcgcc gggccgctca tttcaggttt cagctcagca atcagcgcca
    13501 tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg aatatccagc gggggcttgc
    13561 cctgtgcgct ggcttcttta atgcgcgcgg tcagatccat ctccagcgga tggtcgtaca
    13621 gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg caggtaatca cccagcggat
    13681 tattttctgc cagctcgcgc agacgctcgg cgcggcggtt gtataaattc ttgagccgag
    13741 ggaacaataa cggcggaatc atatccgccg tacgtttctc gctcgaaccc agctcatctt
    13801 gcgggattat gcgaatactc attcagcttt cttttccgtt gtcttgcgga cctcacggta
    13861 ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc catccttcca ccatggcggt
    13921 aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc atgataacca caattaacgc
    13981 cactgcggca aatgaatgca gcattaacgc gaatcggatc accgggattg agaaagcagg
    14041 cgcaaaataa ggacgccaga taatcacgcc gctcaccagc agcagaacca ggaaaataat
    14101 cgccgcccag aaaacgcatt tctgaccgaa gttataacgc ccggtgtcac ctacttcctc
    14161 gttgacgacg atcttacgaa tattcttcgc ccaaaagata tcatcccgat tgattaggtt
    14221 gtgatgccag taacggaaaa acatgatgat gaacgaggca aacataacca cgccgacaaa
    14281 cgggtgcaga attcgcgcca gctgcggtgt gcccatgatt tgcatcaacc agttgaagga
    14341 cgggaacaaa aagcccagcc cgctcactgc cgccaggatg aagcagaagg cggtgatcca
    14401 gtggttgata cgttccggcg cggtgtagcg cacgatggtg tcacgttgtt tcatttgcgc
    14461 tcctcgtctt tctcttcgtg cagattattc tcttcctcat ccgcacggtt cggaccgaca
    14521 cccacgtagt ggaagatact ggctgcgaag gtagccgcaa agccaacagc tgcgagcggt
    14581 ttccagatgc ctttccagaa tttcacggtt tcgctgattt ccgggttctc cggcaagcca
    14641 tgatacagat ttggcttgtc agcatggtgc agcacgtaca taacgtgtgt accaccgacg
    14701 cctgccggat cgtacaggcc cgcattgtcg taaccgcggg ttttcagctc cgccacgcgc
    14761 tcgctcgcca gcgttttcat cgactctttc gtaccgaagt gaatcgcgcc cgttgggcag
    14821 gtcttcacgc aggctggttc ttgcccaacc accacgcggt caacgcacag cgtacatttg
    14881 tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga acggacagcc cgcaatgcaa
    14941 taaccgcagc caatgcactg ctcggactgg aagtcgacga taccgttggc atactgaata
    15001 attgcacctt ccgctgggca cgctttcagg cagcctggat cggaacagtg catacagccg
    15061 tctttgcgga tcagccattc cagtttgtcg ttctgctcca cttccgagaa gcgcattacc
    15121 gtccacgatt tggcgcttaa atcattgggg ttgtcgtaca ccccaatgtt attgccgacg
    15181 gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac aggctttaca gccgatacag
    15241 gtggtgacgt cgatgagttt cgccacttct tcctggaagt cccgcgcctg aggcgcgggg
    15301 gtcagaccgt tagtcgcgga acgacgaatg atatcttgag attgataagc catatgtcgt
    15361 ctccgttaca ccttttccac attcacaagg aaggacttaa actccggcgt ctgcgtgttc
    15421 gcatcaccaa cgaatggcgt taacgtattg gcaataaagc cttttttagc tacgccttca
    15481 tagccccagt gaatcggaat accgatggta tcgatatctt tgccgttcgc tttcagcgtg
    15541 cgaatacgtt tggtcaccac cgctttggct ttgatatagc cacggttgga ggagactttc
    15601 acggtatcgc cctgggcaat gccaagttta ttcgccagcg actccccgat ttccacaaac
    15661 tgctctggtt gcaaaatcgc gttcaacagc gcgtgtttgg tccagtagtg gaagtgctcg
    15721 gtcagacgat aggtggttcc gacatacggg aacttatcgg ctttacccaa tgcttcggcg
    15781 tcgtctttaa agatacgtgc agccgggttc gagataacgt ttggatgcag cgggttagtt
    15841 cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg gaccttccgc catcttatcg
    15901 agggcaaaca gacgccccat gccttcctgc tgcatgataa acggcccgac gccgctaccc
    15961 ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc acttagtgcc gtcccatttc
    16021 agcaactgac gcttcggatc ccacgggtta ccctgcggat ctgcggaggc gcggttatac
    16081 agaatgcggc ggttaagcgg ccatgcccat gcccagccca gcgtgttacc gaggccagac
    16141 ggatcggcgt tatcacgacg cgccatctgg ttgccttccg gcgtccagct accggcgaaa
    16201 atccagcagc cacaggaggt tgtaccgtca tcgcgcaatt gggcgaacga gctaagttgt
    16261 tggcctttct tgacgataac cgcaccggtt gccggatcgg taatatcggc cagcgcctta
    16321 ccgttgctct ccatcgctac ttcttccgac ttcggctcat gcggaatggc gtagttccag
    16381 gtcatgttca gcacctgatc cgggttcgcg ccaccctgtt cggcatacat cttgcgcaag
    16441 cgcaggaaga taccggagag gatctcaccg tcagtcagcg caatccccgg ggcgtccgca
    16501 cctttccagt gccactgcaa ccagcgacct gagttaacga ttgaaccgtt ctcttctgcg
    16561 aagcaggtcg atggcagacg gaacacttcg gtctggatct tcgacgagtc aacttcgttc
    16621 agctcaccgt ggttctgcca gaagttagag gtttcagtgt tcagcgggtc gatggtgacg
    16681 aggaacttca gtttcgacag acaaccgatc actttgtttt tgttcgggaa tgaggcaaca
    16741 gggttaaagc cctggcagat atagccattg accttgcctt ctttcatcat ctcgaagtat
    16801 tgcaggacgt cgtagccttt atcccacttc ggcaaccagt caaagcccca gctattttcc
    16861 gccgtcgctt tatcaccaaa gaaggccttc atcatagaga cgaagaattt cgggtagttg
    16921 ccccagtagt ttacctggcc ttccagcagc ggttttggcg tgttggcggt aaggtaggtt
    16981 tgcagatcgg tctgcttctc gcttggcagc gtcatgtaac ctggcagact ctgcgacagt
    17041 agccccaggt ccgtcagccc ctgaatattg gagtgaccgc gcagggcgtt aacgccgccg
    17101 ccggccatcc ccatattgcc gagcagcagc tggatcatcg ccatcgtacg aatgttttgc
    17161 gcaccaacgg agtgttgcgt ccagccgagg gcatacagga acgaggcagt tttatcgtga
    17221 gcactggttt ctgcgatata ttcgcagact ttcaggaacg cgtcttttgg cgtaccacag
    17281 atgttttcaa ccacatctgg cgtgtagcgg gaaacgtgct gtttcagcaa gttccacacg
    17341 cagcgcggat gttgcagcgt ggtatcgcgt ttggcgaagc cgttttcgtc cagttcataa
    17401 gtccaggagg atttatcgta cttgcgtttt tccgcgtcgt agccggtgaa caggccatct
    17461 tcaaagccgt aatcctcacg cactatcagg ctggcgttgg tataggcttc ggtgtattcg
    17521 cggttgaatt tttcattgtt cagcaggtac agcaatacgc ctgacaggaa agcaatgtca
    17581 gtaccggaac gaataggggc atagtagtca gctaccgccg ccgtacgcgt aaagcgagga
    17641 tcgatcacaa tcagcttcgc gccgttgtga attttggctt ccatcgccca gcggaacccg
    17701 accgggtgag cttcagcggc gttaccgccc atcaccacga cgaggttggc gttcttgatg
    17761 tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt
    17821 ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca tacccagcgc gcgggagaat
    17881 ttttgcgtta aatagccggt ttcgttactc gacgcggaag cacacagcat cccggtggag
    17941 agccagcggt taacagtcac gccttcggcg ttttgcgcaa tgtagttagc atcgcggtct
    18001 tctttcatca gtttggcgat gcgatcaaac gcctcttccc aactgatttg ctgccattta
    18061 tcagaacctg gcgcacggta ttccggaaac ttcagacggc tttcggagtg gatgaaatcc
    18121 accaggccag cgcctttcgg acaaagtgca ccgcggttga ccgggtgatc cggatcgcct
    18181 tcgatatgga agatagatgc tttggcgttt tttgctccgt caccgaggct gtacatcaac
    18241 agcccacagc ctacggaaca ataggtgcag gtattacggg tttcgcgggt gcgcagcagt
    18301 ttatactgcc gggtttccgc gagtgctacg ctgggtgcaa aacccagtgc cgctgccgtg
    18361 gtgcctgcca taccgccagc gcagatctta aagaactgcc ttctgctgac ctgcatggat
    18421 tgctccttgt ttcgacattg tcacgtccca tttacattcg cttgctgcat gtgcagggag
    18481 tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc agaattggct caatttccct
    18541 ccatccagga agggtttgta acagaatacc ataatattgg tgtgtgtgtt cttatctggt
    18601 taagagaaag tgaaaaaaac acagcgaaaa gaaatcgaaa atgtgacaaa tatcacaggt
    18661 gttcgtcaaa ttgagttatg ccggcgtgat gatttacaac acccacggtt agatgaggtc
    18721 gcggaagaag ttcccgttgc gctggtctac aacggcattt cgcatgtggt gatgatggcg
    18781 tcgcccaaag accttgagta ctttgcgctc ggtttttcgc tttccgaggg gattatcgaa
    18841 agtccgcgcg atatcttcgg catggatgtc gttccttcct gtaatggtct tgaagtacaa
    18901 attgagcttt ccagccgccg ctttatgggg ttgaaggagc gccgccgggc gctggcggga
    18961 cgtacgggct gtggcgtatg cggcgtggag caacttaatg acatcggaaa accggtgcag
    19021 ccgctaccgt tcacccagac gtttgatctc aacaaactgg atgatgcatt acgtcatctc
    19081 aacgattttc agccagtggg gcaactgact ggttgtactc acgccgctgc ctggatgttg
    19141 ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc gccatgtggc gctggacaaa
    19201 ctgttaggcc gccggtcaca agaaggggaa agctggcagc aaggtgcggt actggtttcc
    19261 agtcgtgcca gttatgaaat ggtgcaaaag tcggcgatgt gcggcgtaga gattttgttt
    19321 gcggtgtctg ccgcgaccac gcttgctgta gaagtggctg agcgctgtaa tctgacactg
    19381 gtaggttttt gtaaaccggg tcgggcaacg gtttataccc atccgcagcg tttgagcaat
    19441 taattaaaat aagtcctgcg atatatatta aattgcagga cttattcatt tcgtgaattt
    19501 tattatttta tttataaata aaacgcgtgt agtggcgttt tcttttt
//
LOCUS       DAJSKJ010000064        18910 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000064 DAJSKJ010000000
VERSION     DAJSKJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.064"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(49..2610)
                     /gene="mutS"
                     /locus_tag="ON345_001978"
     CDS             complement(49..2610)
                     /gene="mutS"
                     /locus_tag="ON345_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3072039.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2686..2820
                     /locus_tag="ON345_001979"
     CDS             2686..2820
                     /locus_tag="ON345_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072040.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            2897..3250
                     /locus_tag="ON345_001980"
     CDS             2897..3250
                     /locus_tag="ON345_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR3072041.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3287..5365)
                     /gene="flhA"
                     /locus_tag="ON345_001981"
     CDS             complement(3287..5365)
                     /gene="flhA"
                     /locus_tag="ON345_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR3072042.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDIRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(5439..6449)
                     /gene="hypE"
                     /locus_tag="ON345_001982"
     CDS             complement(5439..6449)
                     /gene="hypE"
                     /locus_tag="ON345_001982"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR3072043.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6446..7567)
                     /gene="hypD"
                     /locus_tag="ON345_001983"
     CDS             complement(6446..7567)
                     /gene="hypD"
                     /locus_tag="ON345_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR3072044.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(7567..7839)
                     /gene="hypC"
                     /locus_tag="ON345_001984"
     CDS             complement(7567..7839)
                     /gene="hypC"
                     /locus_tag="ON345_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR3072045.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(7830..8702)
                     /gene="hypB"
                     /locus_tag="ON345_001985"
     CDS             complement(7830..8702)
                     /gene="hypB"
                     /locus_tag="ON345_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR3072046.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(8706..9056)
                     /gene="hypA"
                     /locus_tag="ON345_001986"
     CDS             complement(8706..9056)
                     /gene="hypA"
                     /locus_tag="ON345_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR3072047.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9268..9729
                     /gene="hycA"
                     /locus_tag="ON345_001987"
     CDS             9268..9729
                     /gene="hycA"
                     /locus_tag="ON345_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR3072048.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            9854..10465
                     /gene="hycB"
                     /locus_tag="ON345_001988"
     CDS             9854..10465
                     /gene="hycB"
                     /locus_tag="ON345_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR3072049.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10462..12288
                     /gene="hycC"
                     /locus_tag="ON345_001989"
     CDS             10462..12288
                     /gene="hycC"
                     /locus_tag="ON345_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR3072050.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12291..13214
                     /gene="hycD"
                     /locus_tag="ON345_001990"
     CDS             12291..13214
                     /gene="hycD"
                     /locus_tag="ON345_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR3072051.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13232..14941
                     /gene="hycE"
                     /locus_tag="ON345_001991"
     CDS             13232..14941
                     /gene="hycE"
                     /locus_tag="ON345_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR3072052.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            14951..15493
                     /gene="hycF"
                     /locus_tag="ON345_001992"
     CDS             14951..15493
                     /gene="hycF"
                     /locus_tag="ON345_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR3072053.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15493..16260
                     /gene="hycG"
                     /locus_tag="ON345_001993"
     CDS             15493..16260
                     /gene="hycG"
                     /locus_tag="ON345_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR3072054.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16257..16667
                     /gene="hycH"
                     /locus_tag="ON345_001994"
     CDS             16257..16667
                     /gene="hycH"
                     /locus_tag="ON345_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR3072055.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            16660..17130
                     /gene="hycI"
                     /locus_tag="ON345_001995"
     CDS             16660..17130
                     /gene="hycI"
                     /locus_tag="ON345_001995"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR3072056.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17173..17916
                     /locus_tag="ON345_001996"
     CDS             17173..17916
                     /locus_tag="ON345_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072057.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            18090..18401
                     /locus_tag="ON345_001997"
     CDS             18090..18401
                     /locus_tag="ON345_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR3072058.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18398..18817
                     /locus_tag="ON345_001998"
     CDS             18398..18817
                     /locus_tag="ON345_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3072059.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
BASE COUNT         4171 a         5049 c         5215 g         4475 t
ORIGIN      
        1 cagttgtcgt taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact
       61 cttcaagcga taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt
      121 ttccagtgct tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt
      181 accatccact tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg
      241 ccgtgcgcgc ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta
      301 gcttttgctc gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc
      361 cagcgcatcg agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc
      421 gaaatagtgg gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca
      481 cgcccacgcc agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa
      541 caccagactg tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat
      601 aaaggttgag cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc
      661 gataggtcca atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc
      721 aatcagtgcg gtctggcgca tataggtact tttaccgccc atgttcgggc cggtaataat
      781 caacatacgg cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac
      841 ttgttcaact accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt
      901 cgggcaggtg tagttcaggg tataggcccg ttccgccagg ttaaccagca cgtcgagttc
      961 cgccagcgcg ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacag
     1021 ctcttcataa agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc
     1081 ttcgtactct tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg
     1141 catgtagttg atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg
     1201 caccgcatta aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc
     1261 cagacgctcc agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc
     1321 gttatagccc gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat
     1381 tgctcgctcc agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg
     1441 taccggtgca ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc
     1501 gtggcgcata cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat
     1561 acgttccagg tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa
     1621 tgcgccaata gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag
     1681 ccagcgtttc agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag
     1741 cgtattttcc gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc
     1801 atccataatg atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt
     1861 cgtgcgttgg gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg
     1921 cgcgttctcg acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg
     1981 gcgcgcggtg tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc
     2041 aatcagcgac atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg
     2101 cagctctgcc gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct
     2161 gatatccagc gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt
     2221 gtcctgacgc tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac
     2281 aactttgcgc tcaaccggac ctttgctggt cgccggatcg ccaatttgct cgcagatggc
     2341 aacggactct ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat
     2401 ccccgccatc gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag
     2461 cagttgcgac gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta
     2521 aaacagcagg atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt
     2581 atgggcgtcg aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat
     2641 ggggtgtgat ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta
     2701 atcatttcag gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg
     2761 ttttgcagct tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa
     2821 acctcctcct tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta
     2881 agagtatgga ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa
     2941 aatgatcgat ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga
     3001 ggcgttgttt gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc
     3061 agcctgtaag cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca
     3121 gattatgcgt tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca
     3181 tctcatcgat gacaagcgtc cggtgccaga actgccggaa aaatatcgtc cgaaaaaacc
     3241 tcgtgaatag tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg
     3301 atttatcaat tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac
     3361 gttgcgcagc gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgaacaa
     3421 tcaactgata ttcatcttcg ccctcctgag cgacaaccgt tgcggcaggc ggcgtttcag
     3481 gctccggtaa agcaatatct ggcaatgaca gctgcagcac gttgccgcgt gttagcaata
     3541 ctgcgcgctc aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca
     3601 aggtgcgcag cgtctcggct ggaatgctgt cgatattgcg ccccagacga cgggcaattt
     3661 tgaaggtaaa ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca
     3721 ggtgaatcgg gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga
     3781 ccattttttt cagatcgcgg ttagtcgcgg caattaaacg tatgtccgtc tgaatgattt
     3841 tgttgctgcc gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta
     3901 actccagtgg catatcgccc acttcgtcga ggaacaggga gcttttatcc gccagttcaa
     3961 aacgaccaat gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat
     4021 cgctttccag taatccggca ggcatcgccg cacagttcat tttgaccatg cggcgattat
     4081 tacgcccact gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac
     4141 cgaggatcag cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt
     4201 acatggcttc gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct
     4261 cggtcagggc gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg
     4321 cgagggcgtt atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat
     4381 tggtagtggt aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt
     4441 cgccagacat cagcggtaac aggcacaagg tttgaatctg gttgccccag gtgtcgaaca
     4501 acatgcgttc atagggggct aaatcgtccc gctcgtggag attgatcagc agcatctctt
     4561 tacttttgaa cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg
     4621 cgggatgttg tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc
     4681 tgcgtaagac aatgctgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt
     4741 cgctgaccag ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga
     4801 tgcggaagtt atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc
     4861 tctggatttg ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact
     4921 ctttttcgct ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc
     4981 cttccgccgc cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcgg
     5041 ccagctgcgg ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg
     5101 acaaaatgct gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggg
     5161 tgtctttttc acgcgacgcg taataagacg cacgttgagt ctgcgcttgc cacaacacaa
     5221 tagccgcgtt gtcggcgagc gcagaacgct ttaccagttg cgaaagagcc tcacacagcg
     5281 aggccagatc gggctgctgc aatagtgtcc gagtgatgtc gaacaaccct tgctgtccga
     5341 gatcactcat cggtgtatat gacattttgt tcttccagga aagcaccgca aagcggtgca
     5401 aaaggtcatt gactaactat aggagattta gaattttatt agcatatacg cggaagcggt
     5461 tcggcgtgtg gtaaatcgag ggttcgtttc acgccataca gaccggcaag acgaacacct
     5521 ttacgttcca ccacttcacc aatcagcgcc gcgtctttcc ccagtggatg ggaatgtaac
     5581 gctgccagca cttgctctgc cgcgttgcgt tcaacggcga tcaccagttt gccttcgttg
     5641 gcaaagttca gggcgtccag tcccagcaat tcgcaaacgc cgcgcacggc aggtttaacc
     5701 ggcagcgctg attcagaaat ttcaatacca caaccgcagg ctgccgcgaa ctcatgaacc
     5761 accgcgttta caccaccacg ggtggcatca cgcagcgctt tcacgccggg aatgtcacgc
     5821 agcgtctgaa taagcggcgt cagcaccgcg cagtcgctga ccagttcgcc atccagcccc
     5881 agctgctcac gcaggttaag gatagtcgcc ccgtggtcgc cgagtgtacc gctaaccagc
     5941 aatacatcgc ctgcggttag cgtctgtgcg ccccagtgaa tattcgccgg aattgcgccc
     6001 atgccagcgg tgttgataaa cagtttatct gccgcgccgc gctgcaccac tttagtatcg
     6061 ccagtaacga tggcaatgcc tgccgtgcgg gcggtttctg ccatgctggt cactacggct
     6121 ttcagtgtct ccatcggcaa tccttcttcg aggataaagc cacaggagag atagcgcgga
     6181 atagcgccac tgaccgcaac gtcattcgcg gtgccgcaaa tcgccagctt gccgatatta
     6241 ccgccaggaa agaacagcgg gtcaatgacg taactgtcgg tggagaacgc cagacggtcg
     6301 ccttccgcta ccagctgcgc cagatcaaga cgtgcctgat cttcctgctc tgccagccac
     6361 gggttggcaa aggcttccat aaacaggctg ttgattaatt gctgcatcgc ctggccgccg
     6421 ctgccgtggg cgagttggat attattcacg cttcactctc ctgctgacga tactgatacc
     6481 acgcggcgca cgctccttcg gaggagacca tcagtgcacc aaatgcggtt tgaggattac
     6541 aggtgttacc aaacagcggg cattgatgcg gcttacattt gcccgttaat acctcaccac
     6601 aacgcgcgcg cgggtcatcg cagacctgct gcggtgccgg gcggaaatgt gcttcggcat
     6661 cgaatcgttg ataatccggc gtcaggtgca cgccagaaga ttcaatcacg cctaagccgc
     6721 gccattcgct gtcgccgttg acacagaaca catcggcaat cgcctgttgc gccagcaggt
     6781 taccggcatc cggtaccact cgacgatact gattctctac cttgctgtgg gccgctattt
     6841 tctgctccac cagcatcacc acgccttgca gtagatcaag gggttcgaaa ccagcgacca
     6901 ccagcggacg atgaaaatcg ctggcgataa aattataggc atcagtgccg ataaccatac
     6961 taacgtggcc cggcgcgagg aacgcgtcga taccgttatc cggctgttcc agcaaactgc
     7021 gcagcgtcgg gataagcgta atatgctggc agaagaagta aaaattctgc acatcgcggg
     7081 ctttcgcctg ttgcagagtg atggcggtgg tcggcatggt ggtttcaaaa cctaagccga
     7141 agaacaccac tttgcgggtt ggattctcct gcgccagttt caacgcatcc atcggcgagt
     7201 aaacgatgcg cacatcggca ccgcgtgctt ttgcctgcaa cagcgatccc tgtttccctg
     7261 gtacgcgcat ggcgtcgcca aaggtacaga agatgacttc cggatggctg gcaatctcca
     7321 cgcaggtgtc gattctaccc atcggcagta cgcacaccgg gcaacccgga ccgtggataa
     7381 actcaacgtt ttcaggcagt aactggtcga ggccgaattt aaaaatagcg tgggtatgac
     7441 caccgcacac ttccataatc cgcagagggc gttcggcggt gtaagagaga tgtgaagcac
     7501 gttcgcgcag atgctcaatt aactgcatca cctgttccgg cgcgcgatat tcatcaacaa
     7561 aacgcattat ttttcctcgc catacaacag cgcgccgaca tccggctcaa cgtcaaacat
     7621 gttttgtaag gcgtcgagag tgtcgcgtgc ttcggcttca ttaattacgc tcatggcaaa
     7681 gccaacgtgt accagtaccc actggcccac gcgcggctga ccgttttcat cgcagctgcc
     7741 gactaacgtt aaatcgacat cgcgctgaat gccgcagacg tcgacttttg cctggttgcc
     7801 gtcaatggtg cggatctggc cgggaacgcc tatgcacatc gctgtgtctc cagccagttc
     7861 agccactggt ccatcccttc accgctggtg gcggaaataa ggatgatttc aatttctgga
     7921 ttgacttcgc gggcgcaggc gatgcacttc tcaacgtcaa agttgagata cggcaacagg
     7981 tcaactttgt tgagcagcat cagcgaggcg gcggcaaaca tatgcggata tttcagcggt
     8041 ttgtcttcac cttcggtaac ggaaagcacc gccactttgt gtttttcacc gagatcgaag
     8101 ctggccgggc atacgaggtt gccaacgttt tcgataaaca gaataccgtt atcgtccagt
     8161 ggcagacgcg gtgcggcgtc ggcaatcatc tgtgcgtcaa gatggcagcc tttacctgtg
     8221 ttcacctgaa tcgctggtgt gccggtagcg cgaatgcgtg cggcatcgtt cacggtttgc
     8281 tggtcacctt caataaccgc gcacggaacg ctgtctttca ggcgcattag ggtttccgtc
     8341 agcagggtgg ttttaccgga acctgggctg gaaaccaggt tgagcaccag ttgcttgcgg
     8401 gcagcaaagc gcgcgcggtt gcgttcagcc agacggttgt ttttgtccag cacgtcaatt
     8461 tcgacttcca gcatccgacg ctggctcatg cccggtgcgt gagtgcccgc ttcgccatga
     8521 ccgtaatgca ggtcgccttc ttcagtctgg ctgggggtaa attcaggcgc tttaatgccg
     8581 gtgattttca tcttcgggcg tgccgccggg gcaaatggcg cgctacgaaa cgcggaatga
     8641 gggttatgtt catcaccctc gatatacagg ttgccttcac cgcaaccgca tgttgtacac
     8701 atcgctcact cctggtctat ttctatccgc cgaatctgta aaccgtcgtc tgccacaatc
     8761 tgtagcatgt caccatgaca ctgtggacag cggcggacgc gctgggtcaa tagcgtcaca
     8821 tactgttggc atgtttcaca ccagcattcg gcctcttgtt cttcgaggtg cagtttacaa
     8881 ccttccgcca cgctgccgcg gcaaaccaga tcaaaacaaa aggcaagaga gctggtttcg
     8941 acacaagaaa atgcgccaat tttgagccag accccagtta cgcgttttgc gccgtgtttt
     9001 gcggcctgct gttcgatcaa ttccagtgcc cgttggcaga gggttatttc gtgcatatcg
     9061 cctcccatta actattgcca gctacaagca ataattgtgc cagtgttgat tatccctgcg
     9121 gtgaataatg tcgatgatgt cgaaatgaca cgtcgacacg gcgacgaaat tcatctttag
     9181 cttaaaaatc gttttaataa caataaatta aaagttggca cagaaaatgc ttaaagctgg
     9241 catctctgtt aaacgggtaa cctgacaatg actatttggg aaataagcga gaaagccgat
     9301 tacatcgcac agcggcatcg tcgcctacag gaccagtggc acatctactg caattcgctg
     9361 gttcagggga tcacgttatc gaaagcgcgc ctgcatcacg ccatgagctg cgcgccggac
     9421 aaagaactct gtttcgtcct ttttgaacat tttcgcattt acgtcaccct ggcggatggc
     9481 tttaacagcc acaccatcga gtattacgtc gaaacaaaag atggcgaaga caaacagcgg
     9541 attgcgcagg cgcaactgag cattgacggc atgattgatg gcaaggtcaa catccgcgat
     9601 cgcgaacagg ttctggaaca ctatctcgaa aaaatcgctg gcgtttacga cagcttatac
     9661 accgctattg aaaacaatgt gccggtgaat ttaagccaac tggtaaaggg acaaagcccg
     9721 gcagcatgag ctgaggcttt gcccgttttg caggcgttac gcctgtttgg ggatgggcgt
     9781 gtcgatgagt gtcgaaaatg acatttcatc ggcatgtttt cgtcaaaaat gacaatcacc
     9841 tgaggaatgc ctggtgaatc gttttgtaat tgctgactcc acgctctgta tcggctgcca
     9901 cacttgtgag gccgcctgtt cagagacgca tcgccagcac ggcctgcaat caatgccgcg
     9961 cctgagagtg atgctgaatg aaaaagaatc tgcgccgcag ctctgtcacc actgtgaaga
    10021 tgcaccctgc gcggtggtct gcccggttaa cgccatcacc cgcgttgatg gggccgtgca
    10081 gttgaatgaa agcctgtgcg taagctgcaa gctgtgcggc atcgcctgcc cgtttggcgc
    10141 aattgaattt tccggcagcc gtccgctgga tattccggca aacgccaata ccccgaaagc
    10201 gccaccggca ccgcctgctc cggcgcgtgt cagcacattg cttgactggg tgccaggtat
    10261 tcgcgcgatc gccgtcaaat gtgacctttg tagctttgat gaacaaggtc cggcctgcgt
    10321 gcggatgtgc ccgactaaag ccctgcatct ggtggataac accgatatcg cccgcgtcag
    10381 caaacgtaag cgtgagctga cctttaacac ggactttggc gatctcacct tgtttcagca
    10441 ggctcaaagt ggagaggcta aatgagcgca atttccctga tcaatagcgg cgtggcgtgg
    10501 tttgtcgccg ccgctgttct ggcatttctc ttttcttttc aaaaagcgtt aagtggctgg
    10561 atagctggaa ttggcggcgc ggttggtagt ctgtatacgg cagccgcggg cttcactgta
    10621 ctgactggcg cggttggcgt aagcggtgcg ctgtcgctgg taagctacga tgtgcaaatc
    10681 tctccgctta acgcgatttg gctgattacg ctcggtctgt gcggtctgtt tgtcagcctc
    10741 tacaacattg actggcatcg ccacgcgcag gtgaagtgca acggcttgca gatcaatatg
    10801 ttgatggctg ccgccgtctg cgccgtcatc gccagcaacc tcggcatgtt cgtggtaatg
    10861 gccgaagtca tggccttgtg tgcggtgttc ctcaccagca acagcaaaga gggcaaactg
    10921 tggtttgcgc tggggcgtct tggcactctg ctgctggtga ttgcttgctg gctgctgtgg
    10981 cagcgttatg gcacgctgga tctgcgccta ctggatatgc gtatgcaaca gctgccgctc
    11041 ggttccgata tctggctgct cggtgtgatt ggctttggcc tgctggccgg gattattccg
    11101 ctgcacggct gggtgccgca ggcacatgct aacgcctctg cgccagctgc cgcgttgttt
    11161 tctacggtag tcatgaaaat tggcctgctg ggcatgttaa ccctgtcact gcttggcggt
    11221 aatgcaccgc tgtggtgggg gatcgcgctg ctggtgctcg gcatgatcac cgcgttcgtc
    11281 ggtggtctgt atgcgttgat ggaacacaac atccagcgcc tgctggctta ccacaccctg
    11341 gaaaatatcg gcattatcct gctggggctg ggcgctggcg taacgggtat cgcgctcgaa
    11401 caaccggcac tgattgctct tggcctggtc ggtggtctgt accatctgct taaccatagc
    11461 ctgttcaaaa gcgtactgtt cctcggggcg gggagcgtct ggttccgtac cggtcatcgc
    11521 gatatcgaaa aactcggtgg tattggcaag aaaatgccgg ttatctccat cgccatgtta
    11581 gtcgggttga tggcaatggc tgcgctgccg ccactgaacg ggtttgccgg ggaatgggtt
    11641 atctatcaat ccttcttcaa actgagcaat agcggcgcgt ttgttgcccg tctgctgggg
    11701 ccgctgctcg ctgtgggtct ggcaattacc ggtgcgctgg cggtgatgtg tatggcgaaa
    11761 gtctatggcg tcacgttcct cggcgcgccg cgtaccaaag aagccgaaaa cgccacctgt
    11821 gcgccgctcc tgatgagcgt aagcgtagtg gcactggcga tttgctgcgt aattggcggt
    11881 gttgctgcgc cgtggctgct gccgatgctc tctgctgctg tacctctgcc tctggagcct
    11941 gctaacacca ccgtttctca accaatgatc acattgctgc tgattgcctg cccgctgctg
    12001 ccattcatca ttatggcgat ttgcaaaggc gaccgcctgc cgtcacgttc ccgcggtgcg
    12061 gcctgggtgt gcggctacga ccatgaaaaa tcaatggtga ttaccgctca cggttttgcc
    12121 atgccggtga aacaggcgtt tgcgccagtg ctgaaactgc gcaaatggct gaatccggtg
    12181 tcgctggtgc cgggctggca gtgcgaaggt agtgcgttgc tgttccgccg gatggcgctg
    12241 gttgaactgg cggtactggt ggtgattatt gtttcacgag gagcctgaga atgagtgttt
    12301 tatatccgtt aattcaggcg ctggtgttat ttgccgttgc gccgctgctc tccggtatta
    12361 cccgcgtggc gcgcgcccgt ctgcataacc gtcgtgggcc gggtgtgttg caggagtatc
    12421 gtgacatttt caaactgctg ggccgccaga gcgttggccc ggatgcctcc ggctgggtgt
    12481 tccgcctgac gccgtatgtg atggtgggcg tcatgctgac tatcgctact gcgctgccgg
    12541 tggtgaccgt cggttctccg ctgccgcaac tgggtgattt aatcacctta ctgtatctct
    12601 ttgccatcgc gcgtttcttc tttgccattt ctggtctgga taccggtagc ccgtttaccg
    12661 ctatcggcgc gagccgtgaa gcgatgcttg gcgtgctggt cgaaccgatg ctgctgcttg
    12721 gtctgtgggt tgccgcacag gttgccggtt ccaccaacat cagcaacatc accgacaccg
    12781 tttatcactg gccgctgagc cagagcatcc cgctggtact ggcgctttgt gcctgtgcgt
    12841 tcgccacctt tatcgaaatg ggcaaactgc cgttcgacct ggcggaagcc gagcaggagc
    12901 tgcaggaagg cccgctctct gaatacagcg gcagcggctt tggcgtcatg aaatggggta
    12961 tcagcctgaa acagctggtg gtgctgcaga tgttcgtcgg cgtatttatc ccgtggggac
    13021 agatggaaac cttcaccgcc ggtgggctgc tgctggcgct ggtgattgcc atcgtcaaac
    13081 tggtcgtcgg cgtcctggtt atcgcgctgt tcgaaaacag catggcccgt ctgcgtcttg
    13141 atattactcc gcgcattacc tggactgggt ttggctttgc atttttagcg ttcgtctcct
    13201 tgctggcggc gtgattaaag agagtttgag catgtctgaa gaaaaattag gtcaacatta
    13261 tctcgccgcg ctgaatgagg catttccggg cgtcgtgctg gaccacgcct ggcagaccaa
    13321 agatcagctg actgtcaccg taaaggtgaa ctatctgccg gaagtggtgg agtttctcta
    13381 ctacaagcag gggggctggc tgtcggtgct ttttggtaac gacgaacgca aactgaatgg
    13441 tcattacgcc gtttattacg tgctgtcgat ggagaagggg actaagtgct gggtaacggt
    13501 tcgcgtcgaa gttgatgcca acaaaccgga gtatccgtcc gtgacgccgc gcgttccggc
    13561 ggcggtgtgg ggcgagcgcg aagtgcgcga tatgtacggt ttgattccgg ttggtctgcc
    13621 ggatgaacgc cgtctggtgc tgccggatga ctggccggat gaactttatc cgctgcgtaa
    13681 agacagcatg gattatcgtc agcgtccggc accgaccacc gatgctgaaa cctacgagtt
    13741 catcaacgaa ctgggcgaca agaaaaacaa cgtcgtgccg attggtccgc tgcacgtcac
    13801 ttctgacgaa ccgggccact tccgtctgtt cgtcgatggc gaaaacatta tcgacgccga
    13861 ctaccgtctg ttctacgttc atcgcggcat ggaaaaactg gcggaaaccc gtatgggtta
    13921 caacgaagtg accttcctct ctgaccgtgt gtgcgggatc tgcggctttg cccacagcac
    13981 cgcctacacc acgtcggtag aaaacgcgat ggggattcag gtgccagaac gtgcgcagat
    14041 gatccgcgcc attctgctgg aggtggaacg cctgcactcg catctgctca acctcggtct
    14101 cgcctgccac tttaccggct ttgactccgg ctttatgcag ttcttccgcg tgcgtgaaac
    14161 ctccatgaaa atggcagaga tcctcaccgg tgcgcgtaaa acctacggcc tgaacctgat
    14221 cggcgggatt cgtcgcgatc tgttgaaaga tgacatgatc cagacccgcc agctggcgca
    14281 acagatgcgt cgtgaagtgc aggagctggt ggatgtgctg ctgagtacgc cgaacatgga
    14341 acagcgcacc gtcggcattg gtcgtctgga cccggaaatc gcccgcgatt tcagtaacgt
    14401 tggcccgatg gttcgcgcca gcggtcacgc ccgcgatact cgcgccgatc acccgtttgt
    14461 cggttatggc ctgctgccaa tggaagtcca cagcgagcag ggctgcgacg ttatttcccg
    14521 tctgaaagtg cgtatcaacg aagtttatac cgcgctgaac atgatcgact acggtctgga
    14581 taacctgccg ggcggtccgc tgatggtgga aggctttacc tacattccgc accgtttcgc
    14641 gctgggcttt gccgaagcgc cgcgcggcga cgatatccac tggagcatga ccggcgacaa
    14701 ccagaagctg taccgctggc gctgccgtgc cgcgacctac gcgaactggc cgaccctgcg
    14761 ctacatgctg cgcggtaaca ccgtttccga tgcgccgctg attatcggta gcctggaccc
    14821 ttgctactcc tgtaccgacc gcatgaccgt ggtcgatgtg cgtaagaaaa agagcaaagt
    14881 ggtgccgtac aaagaactcg agcgttacag cattgagcgt aaaaactcgc cgctgaaata
    14941 aggaatcgcc atgtttacct ttatcaaaaa agtcatcaaa accggcacgg cgacctcgtc
    15001 ttatccgctg gagccgattg cggttgataa aaacttccgt ggtaagccag agcagaaccc
    15061 gcagcagtgc atcggctgcg cggcctgcgt caatgcctgc ccgtcaaacg ccttaacggt
    15121 tgaaactgac ctcgccacag gagagcttgc gtgggagttt aatctcgggc gctgcatctt
    15181 ctgtggacgc tgcgaagaag tttgcccgac ggcggcgatc aaactgtcgc aagagtacga
    15241 actggcggtg tggaaaaaag aagatttcct gcaacagtcc cgctttgcgc tgtgcaactg
    15301 tcgcgtctgc aatcgtcctt tcgccgtcca gaaagagatc gactacgcca ttgcgctgct
    15361 taagcacaat ggtgacagcc gcgcggaaaa ccaccgcgaa agctttgaga cttgcccgga
    15421 atgtaagcgc cagaaatgcc tggtgccgtc cgaccgtatt gaactgactc gccatatgaa
    15481 agaggccatc tgatgagcaa tttattaggc ccccgtgacg ccaacggcat tccggtcccg
    15541 atgacggtgg atgaatccat cgccagcatg aaggcgtcgt tactgaaaaa aatcaaacgt
    15601 tctgcctatg tttaccgcgt ggactgcggc ggctgcaacg gctgcgaaat cgaaattttc
    15661 gccactttat cgccgctgtt tgatgcagaa cgcttcggca ttaaagtcgt tccttcaccg
    15721 cgtcatgcgg atattttact gtttaccggc gcggtcaccc gtgcaatgcg atcccctgcg
    15781 ctgcgtgcgt ggcagtccgc gccggacccg aaaatttgta tctcctacgg tgcctgcggt
    15841 aacagcggcg ggatcttcca cgatctctac tgcgtgtggg gcggtacgga taaaattgtc
    15901 cctgtggatg tttatatccc tagctgcccg ccaacgcctg ccgccacgct gtacggcttt
    15961 gcaatggcgc tcggcctgct ggagcagaaa attcacgccc gtgggccggg tgaactggat
    16021 gaacaaccgg cggagatcct gcatggcgat atggtgcagc cgctgcgcgt gaaagtggat
    16081 cgcgaagcac gtcgcctggc gggttatcgt tacggtcgtc agattgccga tgattacctt
    16141 acacagttag ggcagggcga agaacaggtt gcacgctggc tggaagcgga aaacgatccg
    16201 cgtctgaacg agattgtcag ccatctgaat catgttgttg aagaggcgcg tatccgatga
    16261 gtgaaaaggt ggtgttcagt caactgagcc gtaaatttat tgatgagaac gatgccacgc
    16321 ccgccgaggc gcagcaggtg gtctattaca gcctggcgat tggtcaccac cttggggtta
    16381 tcgattgcct ggaagcggcg ctcacctgcc cgtgggatga atatctggca tggattgcca
    16441 ctctggaggc aggcagtgaa gcccgccgca aaatggaagg cgtgccgaaa tatggtgaga
    16501 tcgtcatcga cattaaccat gtgccgatgc tggccaacgc attcgataaa gcccgggcag
    16561 cgcaaacttc gcagcagcag gaatggagta caatgctgtt aagtatgctg catgatattc
    16621 atcaggaaaa cgccatctat ttgatggtga ggagactgcg tgactgacgt tttactctgt
    16681 gttggcaata gcatgatggg cgatgatggc gctggcccgc tgctggcgga aaagtgcgcc
    16741 gccgcgccga aaggtaactg ggtggtgatt gacggcggta gtgcaccgga aaacgacatc
    16801 gtcgccatcc gtgaactgcg cccgacacga ctgctgattg tcgacgccac ggatatgggg
    16861 ctaaaccccg gcgaaatccg catcatcgac ccggatgata tcgccgagat gtttatgatg
    16921 actacccata acatgccgtt gaattacctt atcgaccagt taaaagaaga cattggtgaa
    16981 gtgattttcc tcggcattca gccggatatc gtcggctttt actacccgat gacccagccg
    17041 attaaagatg cggtagaaac cgtttatcaa cgactggaag gctgggaagg aaatggtgga
    17101 tttgcgcagt tagcggtgga agaagaataa ttctccatca gggaattcag ggaattgaac
    17161 atcagaacag ggatgtgcgc ttttttattg agcttagtgc tgcctgcaca ggccaccagc
    17221 tttaccgaat atctgccaat gagtgacagc gaatacgcgc aaaagcgggc gctgaaacct
    17281 ctcctgacca tgccttatga tgccgagcaa acctggcatt ttaggaaagt gggcgttgct
    17341 ggcgtgacgc ttgagaagat gccaaatgaa gaagattact ggcaattaac cgctaaagac
    17401 cgcgccggta aatcctggac tgtgcctgtg ggtatgctgg caaacatagc gggcaaaggg
    17461 caactctatc gcgccgatct tgatcgtaac ggcattcagg atctggttat ctggttacca
    17521 agtaccggta atggtctggc tccttatgca catttaattc ttatgacgtt cacgcgcgaa
    17581 ggccgtccct gtgtctttga acccagggga ttctacaccg caagcaaaac tggcgtagat
    17641 gatttgctgg atttgcaagg caatggtcac acgcaattgc tggatatgca gtttaattct
    17701 ggttactgga taaccagtct gtatcaggta aaagacgcca aatggcagcg tgttcacggc
    17761 tggtttggca agttaagcta tcccgcgctc acgcgtttta cttatacccc caaccgtaaa
    17821 ctcgtcctca aacccatcgc cgggcgcgac ccgcaaacgg aagatctggc gcagacgcag
    17881 cgttgcttga taaaggggga tgtgcttgag gggtgaacca gaactgactt tttgcagagt
    17941 ggctatgagg attttggggg taattatatc gcgcacaagt gtttacatat tgtgaacttg
    18001 gatttgatga gctatagttt ccgaacacat aaggatagaa catcgcagat gtggctgttt
    18061 acttactata aggaagttaa aacttaaaaa tgcacatcat ttctaaagcg ccatttgaag
    18121 agtctgcgcg taaatatcct aacgacgctc tggctcttca ggcattgtat cgggttatta
    18181 aggaaacgga tttttctaca ccagaagaaa tgcgaacggc gtttcccaac ctcgacaatt
    18241 ttagatatcg caacaaatgg tacgttctgg atgtcggcgg gaacaatctt agggttattg
    18301 cctacataaa ctttgttaat aagcggttct ttgtgaagca cataacgaat cacgcagagt
    18361 atgacaaact gacacgttac tacagagaga acaaagaatg actgctaatg ctgcacgcgc
    18421 tgttaaagca acaagagaac tggtcaatgc agtccccttt ctgggaggca gtgactcaga
    18481 agatgattat cgtgaagcac tggaactggt cgaatatttg attgaagaag atgacaccaa
    18541 tccacttatc gactttctgg cgagccgcat tgcagagtat gaaaacaaca acgaaaagtt
    18601 tgcagagttc gacaaagccg ttgcggcaat gccggttggc gtggcgttac ttcgcacgtt
    18661 gattgatcaa cataatctga cctatgcgga tttgaagaac gaaatcggct ctaaatccct
    18721 ggtcagtcag attttgtctg gacaaagatc cctgacgata tcccacatta aggcactttc
    18781 agcccgattt ggcgttaagc cggaatggtt tctttgattt ttgactaatg ccaaatgcta
    18841 aattgcccga tgcgctgcgc ttatcgggcc ttcctggttc gtgcgacatg taggccggat
    18901 aaggcgttct
//
LOCUS       DAJSKJ010000065        18814 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000065 DAJSKJ010000000
VERSION     DAJSKJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.065"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..912
                     /locus_tag="ON345_001999"
     CDS             <1..912
                     /locus_tag="ON345_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR3072060.1"
                     /translation="FYLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIV
                     RAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHNQWSETLEFWPRKPEPGK
                     DTFQYHVLPFDSIDIISKRRESLEDQWSIEDSESYCALMEHFLSGDHGANTFKANMEE
                     APEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIE
                     EELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDK
                     QFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(1029..2372)
                     /gene="gdhA"
                     /locus_tag="ON345_002000"
     CDS             complement(1029..2372)
                     /gene="gdhA"
                     /locus_tag="ON345_002000"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR3072061.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2608..2880
                     /locus_tag="ON345_002001"
     CDS             2608..2880
                     /locus_tag="ON345_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR3072062.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2846..3253)
                     /gene="nudG"
                     /locus_tag="ON345_002002"
     CDS             complement(2846..3253)
                     /gene="nudG"
                     /locus_tag="ON345_002002"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR3072063.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            3340..3960
                     /locus_tag="ON345_002003"
     CDS             3340..3960
                     /locus_tag="ON345_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR3072064.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3969..5276)
                     /gene="ynjE"
                     /locus_tag="ON345_002004"
     CDS             complement(3969..5276)
                     /gene="ynjE"
                     /locus_tag="ON345_002004"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR3072065.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5343..5996)
                     /locus_tag="ON345_002005"
     CDS             complement(5343..5996)
                     /locus_tag="ON345_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3072066.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5996..7531)
                     /locus_tag="ON345_002006"
     CDS             complement(5996..7531)
                     /locus_tag="ON345_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3072067.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLSWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILAVQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7504..8670)
                     /locus_tag="ON345_002007"
     CDS             complement(7504..8670)
                     /locus_tag="ON345_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3072068.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(8680..9228)
                     /locus_tag="ON345_002008"
     CDS             complement(8680..9228)
                     /locus_tag="ON345_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR3072069.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9228..9935)
                     /locus_tag="ON345_002009"
     CDS             complement(9228..9935)
                     /locus_tag="ON345_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3072070.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9949..10626)
                     /gene="ydjY"
                     /locus_tag="ON345_002010"
     CDS             complement(9949..10626)
                     /gene="ydjY"
                     /locus_tag="ON345_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR3072071.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(10631..11341)
                     /locus_tag="ON345_002011"
     CDS             complement(10631..11341)
                     /locus_tag="ON345_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3072072.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(11508..12314)
                     /gene="xthA"
                     /locus_tag="ON345_002012"
     CDS             complement(11508..12314)
                     /gene="xthA"
                     /locus_tag="ON345_002012"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR3072073.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12760..13980
                     /gene="astC"
                     /locus_tag="ON345_002013"
     CDS             12760..13980
                     /gene="astC"
                     /locus_tag="ON345_002013"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR3072074.1"
                     /translation="MSQPITRENFDQWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            13977..15011
                     /gene="astA"
                     /locus_tag="ON345_002014"
     CDS             13977..15011
                     /gene="astA"
                     /locus_tag="ON345_002014"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR3072075.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            15008..16486
                     /gene="astD"
                     /locus_tag="ON345_002015"
     CDS             15008..16486
                     /gene="astD"
                     /locus_tag="ON345_002015"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR3072076.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            16483..17826
                     /gene="astB"
                     /locus_tag="ON345_002016"
     CDS             16483..17826
                     /gene="astB"
                     /locus_tag="ON345_002016"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR3072077.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            17819..18787
                     /gene="astE"
                     /locus_tag="ON345_002017"
     CDS             17819..18787
                     /gene="astE"
                     /locus_tag="ON345_002017"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR3072078.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTITHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4434 a         4871 c         5078 g         4431 t
ORIGIN      
        1 ttttatttaa catgtgttgt cgctgtaatg tttgtgttcg cagcgataac atccaacggt
       61 ttgaatgaaa gaggcctgat taccgcggga tgctcttttc tttatctatt aattatgatg
      121 gggcttattg ttcgggccgg ttttaaagca aaaaaagaac aactgcatta ttatcaggct
      181 aaaggtattg agccgctcag tatcgagaag ttacaggcgc tacaattgat cgcaccttat
      241 cgattctatc ataaccaatg gtctgaaacg ctggagttct ggccgcgaaa gcctgaacct
      301 ggcaaagata ccttccaata tcatgtgctt ccttttgact cgatcgatat cataagtaaa
      361 agacgcgagt ctttagagga tcaatggagt atcgaagata gcgaaagtta ttgtgcctta
      421 atggagcatt ttctttctgg cgaccatgga gccaatacct ttaaagcaaa catggaggaa
      481 gccccagagc aggttatcgc cttgttgaat aaatttgctg tttttccctc agactatatc
      541 tctgattgcg ctaataatcg ctccggtaaa tcctcggcga agctaatatg ggcggcggaa
      601 ttatcatgga tgatctcgat atcaagcaca gcttttcaaa acgggacaat tgaagaagaa
      661 ctggcctggc attatataat gcttgcttct cgaaaggcgc acgagttgtt cgaaagcgaa
      721 gaagattatc aaaaaaatag tctaatggga tttctttact ggcatatctg ctgctatcgc
      781 agaaagttaa cggatgctga acttgaagcg tgttatcgtt acgacaagca gttttgggag
      841 cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc atcatccgtt
      901 aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg cctacattag
      961 agcgtaactg caatgaattg tgcccatttg taggcctgat aagcgtagcg ctatcaggca
     1021 tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac aaaaccggca
     1081 atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc aacacaggca
     1141 tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc tttccagccc
     1201 aggcgcgcag cgttttgcgc catttcaagg cctgacgttg ctacaccgcc agcattagcc
     1261 gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc ttcgatggtg
     1321 gtcggcatat ttgccccttc ggcgacggct ttaacgccat tggcgataag ctgatgcgcg
     1381 gcgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac cggtacagac
     1441 cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc tgccactcga
     1501 ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt gaatccgctt
     1561 tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc aaattccatc
     1621 gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac gcgcatccct
     1681 tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag accgtagccg
     1741 gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt gaagacgcag
     1801 gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc acgaccacca
     1861 acgccgatat cacctgccgg aacgtcggta tccgcgccca gatgacgata aagctcggtc
     1921 atcagcgcct ggcagaaacg catcacttcg ccttcgcttt tacctttcgg gtcaaaatcg
     1981 ctaccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa ggtttgttcg
     2041 aagccgagga atttgagaat ggagaggtta actgacggat ggaagcgcat accgcctttg
     2101 tacgggccga tggctgagct gaactgcaca cgccatgcac ggttgacctg tacctggttg
     2161 cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac cagacgctcc
     2221 agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag tgtggtcatt
     2281 acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg gacatggttg
     2341 aggaatgact ccagagaata tgtctgatcc atagatataa aacccttata tattaatacg
     2401 attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac catcaagaat
     2461 gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat taataacgag agtaatctca
     2521 taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt tatcagttgc
     2581 agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt agtattagca
     2641 tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt aaacgtaccg
     2701 ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg ctgcgtttat
     2761 caggatcaaa attattctga aggggcggta attaaagccg aaggaatact gctgcaatgc
     2821 cagcgggatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt aaagccataa
     2881 acgcctctaa tagtggaatg tcagcagggg ccagcggata ttgcagcgcc tcttcaggtg
     2941 agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag tcgggtacgt
     3001 gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg gcaacatact
     3061 cacccacagt tgcttcgatg cccaattctt cgtttaactc acgcaccagt gcctgctgct
     3121 ggctttcatc cagctcgact ttaccaccgg caaactccca taatcccgcc tgatcgctct
     3181 gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg gcaacaactt
     3241 caatcatttt cataaattac acacgggcag ataagcgaaa aatgccataa tatccgcact
     3301 gttgctcaat cagaaaggct ttatatttaa ggagtgcctg tgctagaccg ccatcttcat
     3361 ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc ttgataaacc gggcattacg
     3421 ccagatggtt taacattagt gggatttgcc atcggcgtgc tggctctgcc gtttctggcg
     3481 ctgggctggt atctggcggc gctagtcgtc attttgttga acaggctgct tgatggtctg
     3541 gacggtgcgc tggcgcggcg cagagagctt accgacgcgg gcggctttct tgatatttct
     3601 ctcgattttc tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa
     3661 aatgcgctgg caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc
     3721 gcctttgctg cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg
     3781 ttttactatc tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga
     3841 tgtttattcc ctgcatggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg
     3901 acgaccttta cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa
     3961 ttgcaatgtt atttgctact gtccgggccg cgttcaccgg ttgctaccgg attttttgga
     4021 tcgctgctcc attcgtacca gccgccgtca tacacggaaa cattcttcca acccatggcg
     4081 cgtgcgtaca taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc
     4141 tgctctggtt tgatattcca cgctttccac atagcggtaa tatcatcggc gctgcgcatg
     4201 gtgccatccg ggttatgaaa atcttccata tgcgtcgagt cgctaccagc gtgtccccaa
     4261 cgtgctccgg ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata
     4321 aattctggcc acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgg
     4381 gcctgttcca tatcgagcat caactgcggt tgtgccggga tcttcacgcc gaaatccggt
     4441 tccgctttca ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt
     4501 tgccagccgc catccagcag acgcacatct ttcacgcctg cgtagagcat aatttgcgcc
     4561 acacgcgctc cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg
     4621 ccgtgttttg ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca cagcggttcg
     4681 ctttccactt cgttggtatc gatgtagtct gcaccgggaa tatggctgat aaggtaaagc
     4741 ttaggagcgc cccaggccgc ttcaatgact ttccagtcac cggcaggttt cgccgtaacc
     4801 tctttacctt gttgcaggtc gtgcagccat tgcggataaa ccagctgctc aaagtgcggc
     4861 agtttttgta gacgggaagg ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa
     4921 cctgcttttt gcagtcgcgt tttgacggcg tcgacatctt tgtcattacc gtacagcgcc
     4981 accggggcat cggctttcag gttatgttgc ttgatccacg cgttgagctg ttcggtgctc
     5041 attttgtcga gccagctggc agagaggttt aaggcggcag gttcatgacc agaagggcca
     5101 tttaaggttt gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca
     5161 ttttgttgtt gaagctggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa
     5221 gcgcaagcca gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa
     5281 aatcccgaca ttgtagtttt gagtgcgcca ttctgaactg acgcattgag aaaaactttg
     5341 cgttatcgca gtttgttgta attttctgac cactgcgcca tatccagaac agaactatca
     5401 gcaggaacat cctggagatc gtgcgttacc tgaacgacgg ggatcgccag ttcgcgaact
     5461 tcgctgaaca cccactggcg aaaattatcg cgcagagcca catcaagacg gctgaatggc
     5521 tcatccagga gtaacgcttt tggctgagcg agaagggcgc gcagcagggc cacccgcgct
     5581 cgctgaccgc ctgataatgt cgcagggtcc tggtgataag tttcagccag tcccgctcga
     5641 tcaagcgcgt ctttcacggc attgcgcctg gcagtcccct tgagcgtcga cggtagcgcc
     5701 agcagtaaat tttgcccgac gctgaactgg tcaaataaca gtgcatcctg gaaaagaatg
     5761 ccaatctgac gctgtgcggt gggtagcatg tcaatccgtt gctcattgag ccatagctca
     5821 cctgtacaag aaaactgtcc agccagtgca ccaatcatcc atgaaaacag agtggatttt
     5881 ccacagccag acggccccat taacgtgaca atgtcacctt tatccaccgt aaagttaacg
     5941 tttgtcagca agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc
     6001 ggagtccttg tctgacataa cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa
     6061 taagcggtaa tagcagttgc cataaagcct gggcggcgaa aataccattg ctgccgccgc
     6121 tgcttaatgc caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg
     6181 tcggcatata ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc agcgtagggc
     6241 gtagcataag cggacatttc acgtaaaaga agattttggc ccgcgaccag ctcagtgttt
     6301 gcgcaatcaa aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca
     6361 tccacggcat tacccacagc agatgccccc agaccaccgc cgtccagctt ccatccaggt
     6421 tcagccatag cgccagcgtg tactggcccg ccaccagtgg cagagcgggc aatagaatgg
     6481 gcaaccagag ccacaactgg cgacgctgtg gcccccattc cagccacagc agtaacagga
     6541 gcaaagcgat gaatgtcgcc accagcccca ttgtcaggct gttgatgagt gcttcactat
     6601 tgatcgtcga ctgaaccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg
     6661 gtaaaaaact cgccagcgta gtgcctggta ataaaggtgt ggcaggcttg cgaatgccat
     6721 ctacgcgggg aatagtgcgc cgccagctgc gccatagcag atagctcagc aaaacgtagg
     6781 cggcgagtaa cagcatcagc agcagactgg cgagtgcgcc tttcgtttgt tgatccgcgt
     6841 cgccctgagt taaccactgc cagctaatta ctgccagcgt cggtggatta cctggcccaa
     6901 gaataatcgc cacatccacg accgaaagcg accaggcaac aatcgccagc atcgccattg
     6961 ccagcgaagg cgcgacggag ggcaacagca gccagttcag gcattgccag cggctgtagc
     7021 ccagcgaatc cagcacaatg acctgctgca acagccgttt ttcgctcaat actgccgcta
     7081 agatccacag cagaaatgcg ctttctttca ctgcaagggt gaggcccaga ccgatgccaa
     7141 atcggtccat tggcggagtg aaatacggga aatagtcata tagcagccct ccgtcagcaa
     7201 agagcagcag ggcgctggtg gcaaaagcca catggggaat ggcgagcagc cacgacagac
     7261 gggcgcacat acgctgccat ttcggcccag gccatagcgc cacaatcacc agcagggcaa
     7321 tcaacaatgc cccgaccgcc gcgatggttg tcgacaccag cgttgccagt aatgcatgcg
     7381 gtaactgtgg atcggcaaat aacgcctgcc agtgtgtcaa cgacaacgca ggcgatatca
     7441 gcgtgagagc tgccgggatc agcggtgcat aaatcaccgc caccatcgcc cacagcaaaa
     7501 agattaatgc gtaccgtaac ggcgtaacca ttcttgttcc agtgcattca cccatcctgc
     7561 gtgcggttca gccagtaccg gcggcagatc ctgcggcatt cttgattgca atatttcgcg
     7621 ctgcccgtca ggcagttttt gcggatcgag aacagaagga tcgccccaga caacgggatc
     7681 tgctttacgc agttgcgcat cgggtgagag caggaaattg gcaactacct tcgccgcagc
     7741 actggcattc gcgttggctg gaatggtgac gaaatgcacg ttgccaatca tcccctcgcg
     7801 aaagccaaaa ctgtaactgc ttgccggcaa atcgccgctg gcgatttttt gctgcgcatg
     7861 cgcggggtta aaggtcagcg acaggcgcaa tgtgccggct ttcagcagag catccatccg
     7921 cgcgggcgaa ggcgggaaat cctttccttc gcgccacaaa tacggatgta gcgcatcaag
     7981 atattgccac aagggagcag tgacacgggc gaaagtcgca tcgtccggcg cttcttttaa
     8041 tgctgcggga tcgggcgtca gcataatcag caactgttca agaaacgccg tgccggtaaa
     8101 gtccggtggg cgcggatagg taaccgtgcc gggattgact ttagcaaact ccagtaaggc
     8161 ttgcggcgtt tgtggtggct gtggcgtaac atcgcggcgg gcgataaacg tcagttgtgc
     8221 gccgccccag ggcgattccg caccttgtgt cggcactgaa aaatcttccc gcaccggcag
     8281 ctgtgtgtcg acatagcgcc agttgggcag agtctccgcc cagcccgttt gtaataaatt
     8341 ggcctctttt aaggtgcgga agttttcgcc gttcacccag agcagatcca ccgagccgcc
     8401 cgttttacgt ccggcagcag cttcggtctg aatgcgcttc accgcgtctg cggcatccgc
     8461 gagacgaaca atcttcaggt ttatagcgta atgggttttc atctcgccgc taacccagtc
     8521 gagatagcgg ttaattgcgg tatcgccgcc ccaggcgtta aaccagacgg tttgcccttt
     8581 ggcctcattt ttaatttcct gccagtcact ggcatgtgtt gccaaagaaa acagcgataa
     8641 caatcccagc aaccacccac aatggcgcat catctctcct taagaacgtt tttcaggaat
     8701 acggcacagc ccctgagcgg gaatgtccat ggcagaatta aaacgagccg acaggttttg
     8761 caggccgatc aatgccgtca gttcggtgag cgcctgagcg tcaaaatgcg cagccagtcg
     8821 ggtacgcagg gcatcatcga ctgttggcgg tgtgacgctt gcggcttcgg cgtactccag
     8881 cgccagccgt tcttcatcgc taaagagcgg gctttgccgc cagtcagcca ctgccagcag
     8941 tttatcggtg ctgccggtac gctcggcgac tttcatactg gtgatatcca cgcaaaactc
     9001 acacaggcac atttgcgcaa tgcgcgcgct gacaagcgat cgcactaccg gatcgagcgg
     9061 tgagcgtttg cgctccagcc acccaacaaa cattgacacc aaataaaaga taaacgggat
     9121 ccgcccccac cagcgaatcg ggcttagcac ctcgccgtaa tggcgacgat gcagccacgc
     9181 ctgcggacgt aaaatgaaag gaattttgct aagcggcggt aaacccatca gtcattcctc
     9241 ttctggcgct caagccatat tttcttcgcc ataaaaatca ccacggtcag ggcaaacaga
     9301 ataaacagtc cggtgacaaa ccagaaagta ccgcctgtta acatgctgcc cgcccaggaa
     9361 taaacaatag tcgccggtaa ctgaccaagc ccggtggcga taaaaaacga gcgaaaacgt
     9421 attgaagtca aaccggcagc atagctgatt ggatcgaaag ggacaaaagg caataaccga
     9481 cagaccagga tggtgtgttt gccgtagcga gtgaaaaagc cgtccatact gtcaagtacg
     9541 gttttgccgg ttaatttttc caccacttcg cggcccatca ctctggcgat aaaaaagcac
     9601 agcgccgcgc cggccatcga actggtccac gacagcaagc cgccccagaa cgcgccaaac
     9661 agcgacgcat tggcaaaggt gatcaaaaac gcaggtagcg gtgcagcaat ggcctgtaaa
     9721 atcatcaaca agaacgagac aactgccgcc agtgcgccgt aagactgaat aaagcgttct
     9781 ataccctgtt ggtccacggc ggcaaacgcc gcaacgctgc gattaataaa ctcatggacg
     9841 ccgggaagca gcgcccatgc cagcagcaca gcgaacaaca ggatgatgag ggttatacgg
     9901 tagtaccaga ttttacgcga ttgcatcttc atcatccttt atcctgcttt attcggtgat
     9961 tttaaaggta accgttgcca gcgtgttatc cgccgggaga actgaggcat tgcctttgaa
    10021 tttcacttcg ccgcgttttt ccaccgcacc ataagtgtat gttgcattgc tgacgatgcc
    10081 gaccgggcag ctatccagac acaccaggca acccgttttc ttctcttctg ctgccgttaa
    10141 attaccgcca aagcgcatgt ccagtttctt gccattactg tcaacaatca cttcatcgaa
    10201 ggaatacgct tttgccgccc cttgccagtt gaccgaaata tccagtttgc tgcctgtgac
    10261 atgagtcgtt tctttattat ccatcgtcat gttttcgccc ggcgtaccac ctgcctcttt
    10321 cagggcttca taaaatgctt tcggggtcgc ataagccata aacagcgatt tatgtccgtt
    10381 ggagccatct ttaaacacaa taccgtgacg agtgtcgtcg gtgaggaaac gaccattaat
    10441 ttgcaccagc atggttaccg tatggttatt ggcatcgacg cgcagcggtt ggctgttcga
    10501 aagtccgtcg tattccactt ttgccgccgc attctctttt tgatcgcagc cgctaagccc
    10561 ggcaacagcc agtaagcaga gtgcagccag tccttttttc catgacactg aataatgttg
    10621 cgacatcgtt ctatccttca ttttttgggt gagtaagcgg gctgcggcgc gaagcagaca
    10681 gatccacatg tttgtgtcgg gcgtagagtt ttgcgagctg gacgagaata aacagcgcca
    10741 ggcccgccag acagagttgt aaaataaagc gcagcgtaat gccttcattg gcgagatcgc
    10801 ttgccatcac ggtataaata acaataccgg gcagggtcgt gagtgccgaa ataagggtat
    10861 aaggccagaa ggcgatggtg gttaatccgt aagcgtaatt ttgaatattg taaggaaaca
    10921 acgggattaa gcgggtcaga ataagaaaat caataccgtt acgcgcaatg cctttttcaa
    10981 tggcctggaa ggtatggcta tggcccacgt atttcagcag taaatcacgt cccagccagc
    11041 gcgccagcag gaatgagcac gaggaggcaa gcgtggcggc aattaatgag agtagtgtcc
    11101 ctaagagcgg gccaaaaact attcctccag cgatcaccag tatgcttcct ggtaacagaa
    11161 agagggtggc aatgatgaat aacagaatat agaggctata accgaacaat ccgctctggc
    11221 gaatgagtgt ctgtaagtgg ggtaaatcgg tgagcagatc gaataaaccg aaagcgtgga
    11281 tagcgtaaat gaccagcgca aaaataagac aggcgaaaag aaatttacgc tcagcattca
    11341 tcgtggagtt tcctatatca aacttgataa tagagaagtg gtcagacaac taaaaacgca
    11401 gtgacggtat aacaaaggac ggcaggcaac aaatcattac tgacagtttg ttgatttttc
    11461 tcaaggttaa ttctcctgac ccagtttgag ccaggagagc tgctaaatta gcggcggaag
    11521 gtcgcccaga cgggggcgtg atcggacggt ttttccatgc tgcgaatttc atagtcgatg
    11581 ccggtttcta cgcaacattc tgccagcggt tggctggcga gcagcaggtc gatgcgcaga
    11641 ccacggttat cgtcaaaacc ttttgagcgg taatcaaacc atgagaaacg atctgctgtt
    11701 tgcggattag catggcggaa agtatcgacc aacccccagc tcatcagcct gtccatccat
    11761 tcgcgctctt ccggcaggaa agagcattta ccggtacgca gccagcgctt acggttttct
    11821 tcgccaatgc cgatatccag atctgtaggg ctgatattca tatcgcccat aatcagtacc
    11881 ggattatcac gtttgagttc ggtttccagg tagttttgca gattctgata aaactgcgct
    11941 tttgccggga attttatcgg atggtcgcgg ctttcaccct gcgggaagta accgttgatc
    12001 acggtgacat tacccagcag tgaggggatt tccgccataa taatccgccg ctgcgcctct
    12061 tcgtcgtcac cgggaaagcc gcgacgcacg gcaatcggcg tctctttggt cagcagcgcc
    12121 acgccataat ggcctttctg cccgtgataa aacacgttgt agccaagctt cgccacctct
    12181 tcgagcggaa acatatcgtc atgaactttt gtctcctgca ggccaatcac atccggttgg
    12241 tgcttttcga cgatggcttc aagctggtga ggtctggcgc gcagtccgtt gatattaaaa
    12301 gagacaaatt tcatagtcgc tgccatttat tcagataaag agtgcgtgga tggtagcaga
    12361 atttcgcgtt gcttaccgcc tgttgttaag tgatttgatc gatggttgtt aatgatgtca
    12421 acgatggcgc aaaaaatgcc cgcttttggt gcgcgctgcg tcagaatggc gcagtaattt
    12481 ccagtaaatt tcgtcaatga tcacaattcc agaattatat tttacttctg cataacattg
    12541 cgttttttat tctttttatt cacccacatc gcaaacatat tcactttata tgcactttaa
    12601 atgcatatgg tttggttata actccttgat ttccagttag cctccgccgt ttatgcactt
    12661 ttatcactgg ctggcacgaa ccctgcaatc tacatttaca gcgcaaacat tacttattgt
    12721 taacatataa ataacgaatt atttactgta gaggtcgcta tgtctcagcc aattacgcgt
    12781 gaaaactttg atcaatggat gatacctgtt tacgctccgg caccctttat accggtacgt
    12841 ggcgaaggtt cgcgcttgtg ggatcagcag gggaaagagt atatcgactt cgcgggtggc
    12901 attgcggtga acgcgctggg ccatgcgcat ccggaactgc gtgaagcgct gaacgaacag
    12961 gcgagtaagt tctggcatac cggcaacggt tacaccaacg agccggtact gcgactggcg
    13021 aaaaaattga tcgacgccac gtttgccgat cgcgtcttct tttgtaactc cggtgcggaa
    13081 gccaacgaag cggcgctaaa actggcgcgt aaattcgctc acgaccgcta cggcagccat
    13141 aagagcggca tcgtggcgtt caaaaatgcg tttcatggtc gcacgctgtt tactgtcagt
    13201 gcgggtgggc agccagccta ttcacaggat tttgcgccac tgccggcgga tattcgtcat
    13261 gctgcatata acgatattaa ctctgccagc gcgctgattg acgactctac ctgtgcggtg
    13321 attgtcgaac ccatccaggg ggaaggcggt gtggtgccag ccagcaacgc gtttttacaa
    13381 ggtctgcgtg aattgtgtaa ccgccacaat gcgctgttga tttttgatga agtacaaacc
    13441 ggcgtcgggc gcaccgggga actgtatgcc tatatgcact acggcgtgac gcctgatctg
    13501 ttaactaccg ccaaagcgct gggcggcggt ttcccggtcg gtgcgttgtt ggcaaccgaa
    13561 gagtgcgccc gcgtgatgac cgttggcact catggcacca cctatggcgg taatccgctg
    13621 gcctcggcgg tggcaggcaa agtgctggag ctcatcaaca caccagagat gcttaatggc
    13681 gttaaacagc gtcacgactg gtttgttgag cgtcttaata ctattaatca ccgctatggt
    13741 ttgttcagtg aagttcgcgg cttaggtttg ctgattggct gtgtactgaa tgccgattac
    13801 gccgggcaag cgaaacagat ctctcaggaa gcggcgaaag caggcgtgat ggtactgatt
    13861 gcgggtggca acgtggtgcg ttttgcgcct gcgctcaatg tcagcgaaga agaggtgacg
    13921 accggactgg atcgctttgc agctgcttgc gaacactttg ttagccgagg ttcatcatga
    13981 tggtcatccg tcccgttgag cgatcagatg tctcggcgct gatgcagctt gccagcaaaa
    14041 cgggcggcgg cctgacgtcg cttcccgcca atgaagccac gctttcggcg cgtatcgaaa
    14101 gggcaatcaa aacctggcaa ggcgaactgc ccaaaagtga gcagggctat gtgttcgtgc
    14161 tggaagatag cgagacaggc accgtggcgg ggatttgtgc cattgaggtg gcggttgggc
    14221 tgaacgatcc ctggtacaac tatcgcgtcg gcacgttggt tcacgcctca aaagagctga
    14281 atgtctataa cgcattgccg acgctgtttc tcagtaacga tcacaccggc agcagcgagc
    14341 tgtgcacgct gtttctcgac ccggactggc gcaaagaggg caacggctat ttgctgtcga
    14401 aatcgcgctt tatgtttatg gcggcttttc gcgacaagtt taatgacaaa gtggttgctg
    14461 aaatgcgcgg ggtgattgac gaacacggct attcaccgtt ctggcaaagc ctcggtaaac
    14521 gcttcttttc gatggatttt agccgcgccg attttctctg cggcaccggg caaaaggcat
    14581 ttattgcaga actgatgccg aaacatccga tctataccca ctttttatcc caggaagccc
    14641 aggacgtcat cggtcaggta catccgcaaa ccgcgcctgc ccgcgcggtg ctggagaaag
    14701 aaggttttcg ttaccgcaac tatatcgaca tcttcgacgg cgggccaacg cttgagtgtg
    14761 acatcgaccg cgtgcgcgcc attcgtaaaa gtcggctggt ggaagtggca gaagggcagc
    14821 ctgcgcaggg cgattttccg gcctgcctgg tcgccaatga aaattatcac catttccgcg
    14881 tggtgctggc gcgtaccgat ccggcaaccg agcgtttgat tttaaccgcc gcacaactgg
    14941 atgccctcaa atgccacgca ggggatcgcg ttcgtctggt gcgcctgtgc gcagaggaga
    15001 aaacagcatg actttatgga ttaacggtga ctggataacg ggccagggcg catcgcgtgt
    15061 gaagcgtaat ccggtatcgg gcgaggtgtt atggcaaggc aatgatgccg atgccgctca
    15121 ggtcgaacag gcttgtcggg cagcccgtgc ggcgtttccg cgctgggcgc ggctctcatt
    15181 ggctgaacgt caggtcgttg tcgaacgctt tgccggattg ctggaaagga ataaaggcga
    15241 attaaccgcg attattgcca gagaaacggg taagccgcgc tgggaagcgg caaccgaagt
    15301 gacggcgatg atcaataaaa tcgcgatatc aattaaggcg tatcacgttc gtaccggcga
    15361 gcagcgtagt gaaatgccgg acggtgcggc gagcctgcga catcgcccgc acggcgtgct
    15421 ggcggtgttt gggccgtata atttccctgg tcatttgccg aacggacata tcgttccggc
    15481 attgctggca ggtaacacca ttatctttaa acccagcgaa ctgacaccgt ggagtggcga
    15541 agcggtaatg cgtttatggc agcaggctgg cttgccgccg ggcgtactga acctggtgca
    15601 gggcgggcgt gaaacgggtc aggcgctgag tgcgctggag gatctcgacg gtttgctgtt
    15661 taccggtagc gccaataccg gctaccagct gcatcgccag ctctccggtc agccggagaa
    15721 aattctcgcc cttgagatgg gcggtaataa tccgctaatt atcgatgagg tggcggatat
    15781 cgacgcggct gtccatctga ccattcagtc ggcgtttgtc acagccgggc aacgctgcac
    15841 ctgcgcccgc cgtttattgc tcaaaagcgg agcgcagggc gatgcgtttc ttgctcgtct
    15901 ggttgccgtc agccagcgat taacgccggg caactgggat gacgaaccgc agccgtttat
    15961 tggcgggctg atttctgaac aggccgcaca gcaggtggtt actgcctggc agcaactgga
    16021 agcgatgggc ggacgaaccc tgcttgcgcc gcgcttatta caatcagaga catcgttgct
    16081 gacgccgggg atcattgaaa tgacaggcgt tgctggcgta ccagatgaag aggtgttcgg
    16141 accgttattg cgcgtctggc gttatgattc tttcgaggaa gcgattctaa tggcgaataa
    16201 cactcgcttc ggactctctt gcggtctggt ttcccccgag cgggaaaaat tcgatcaact
    16261 gttgctggag gcgcgggcgg ggattgttaa ctggaacaaa ccgcttactg gtgctgccag
    16321 taccgcgcca ttcggcggca ttggtgcatc cggtaaccat cgccccagcg cctggtatgc
    16381 cgcagattac tgcgcatggc ctatggcgag cctggagtcg gactcgttaa ctttgcccgc
    16441 aacgcttaac cccgggctgg atttttccga tgaggtggtg cgatgaacgc ctgggaagtt
    16501 aatttcgacg ggctggtagg gctgacgcat cattacgctg gcctgtcgtt tggtaatgaa
    16561 gcctctaccc gtcaccgttt tcaggtgtct aacccgcgac aggcggcaaa acagggctta
    16621 ctgaaaatga aagcccttgc cgatgcggga ttcccccagg ccgtgatccc gccgcacgag
    16681 cgtccgttta ttccggtgct gcgtcagttg ggattcagtg gtagcgatga gcaggtactg
    16741 gaaaaagttg cacgccaggc accgcactgg ctttccagcg tcagttccgc ttcgccaatg
    16801 tgggtagcca atgcggcaac gatcgcgcca tctgccgata cgctggatgg caaagtgcat
    16861 ctcaccgttg ccaacctgaa caataaattt caccgctcgc tggaagcgca cgtcaccgaa
    16921 tcacttttaa aagcgatttt taacgacgaa gagaaattta gcgttcattc ggcgttgcct
    16981 caggtagcgt tgctcggtga tgagggggcg gcaaaccaca atcgtctcgg cggtcattac
    17041 ggtgagccgg gtatgcaact ttttgtctac gggcgagaag aaggcaatga tacccggcct
    17101 tcccgttatc cggcgcgaca gactcgcgaa gccagcgagg cggtggcaag gctgaatcag
    17161 gtgaatcccc aacaggtgat tttcgcccag caaaacccgg acgttatcga ccagggcgtt
    17221 tttcataatg acgtgattgc cgtgagtaac cgccaggtgc tgttttgcca ccaacaggcg
    17281 ttcgctcgcc agtcacagtt actggcaaac ctgcgtgcgc gggtgaacgg ttttatggcg
    17341 atagaagttc cggcaactca ggtttccgtg tcggatgcgg tgtctaccta tctgtttaac
    17401 agccaactgt tgagccgcga tgatggttcc atgatgttgg tgctgcctca ggagtgtcgg
    17461 gaacacgccg gagtatgggg ttatctcaat gaactccttg ccgctgacaa cccgattagc
    17521 gaactaaaag tctttgattt acgtgaaagc atggcgaatg gcggcggccc ggcgtgcctg
    17581 cggttgcggg tggtattgac agaagaagaa cgccgggcgg tgaatccggc ggtgatgatg
    17641 aacgatacgc tgtttaatgc gctcaatgac tgggtggatc gttactaccg cgatcgcctt
    17701 actgctgccg atctggccga cccgcaattg ctgcgcgaag ggcgggaagc actggatgta
    17761 ttgagccaat tactgaatct cggttcggtt tatccgttcc agcgcgaggg agggggcaat
    17821 ggataatttt cttgctctga ccttaacggg taaaaaaccg gttatcaccg agcgagaaat
    17881 caacggcgtt cgctggcgct ggctgggcga tggtgtgctg gaactgacgc cattaacgcc
    17941 accgcaaggc gcactggtga tttcagcggg aatacacggt aatgagacgg cacctgtgga
    18001 gatgctggac gcgttgcttg gcgcgatatc tcacggcgag atcccgttac gttggcggtt
    18061 gctggtgatc ctcgggaatc ctcctgcgct gaagcaaggg aaacgttatt gccatagcga
    18121 tatgaatcga atgtttggcg gtcgttggca gctatttgct gaaagcggag aaacctgtcg
    18181 ggcgcgcgaa ctggaacagt gcctggaaga tttttatgac cagggcaaag aatctgtgcg
    18241 ctggcacctt gatctacata ccgcaattcg tggctccttg catccgcagt tcggtgtatt
    18301 accgcaacgc gacattccct gggacgagaa atttctgacg tggctgggtg cggcggggct
    18361 ggaggcgctg gtgttccatc aggaacctgg tggtacgttt acccatttca gcgccagaca
    18421 ttttggcgcg ctggcctgta cgctggaact tggcaaagcg ttgccctttg ggcaaaacga
    18481 tcttcgccag tttgcagtaa ctgccagcgc aattgctgcg ctgctatctg gtgagagtgt
    18541 cggtatcgtg agaacaccgc cgctccgtta tcgggtggtt tcgcaaatta ctcgccactc
    18601 gccgtccttc gaaatgcata tggcaagtga cacgctgaat tttatgccgt ttgagaaagg
    18661 aacattgctg gcgcaggacg gagaggaacg ttttaccata acccatgatg tagagtatgt
    18721 gttattccct aatccgttgg tagcgttggg attacgcgcg ggattaatgc tcgaaaaaat
    18781 aagctaatac ttacccgcag aaatcattct gcgg
//
LOCUS       DAJSKJ010000066        18469 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000066 DAJSKJ010000000
VERSION     DAJSKJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.066"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..596)
                     /gene="rsmG"
                     /locus_tag="ON345_002018"
     CDS             complement(<1..596)
                     /gene="rsmG"
                     /locus_tag="ON345_002018"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR3072079.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHL"
     gene            complement(660..2549)
                     /gene="mnmG"
                     /locus_tag="ON345_002019"
     CDS             complement(660..2549)
                     /gene="mnmG"
                     /locus_tag="ON345_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR3072080.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2928..3371)
                     /gene="mioC"
                     /locus_tag="ON345_002020"
     CDS             complement(2928..3371)
                     /gene="mioC"
                     /locus_tag="ON345_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR3072081.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3461..3919)
                     /gene="asnC"
                     /locus_tag="ON345_002021"
     CDS             complement(3461..3919)
                     /gene="asnC"
                     /locus_tag="ON345_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR3072082.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4071..5063
                     /gene="asnA"
                     /locus_tag="ON345_002022"
     CDS             4071..5063
                     /gene="asnA"
                     /locus_tag="ON345_002022"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR3072083.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLVPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERTIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(5068..6519)
                     /gene="viaA"
                     /locus_tag="ON345_002023"
     CDS             complement(5068..6519)
                     /gene="viaA"
                     /locus_tag="ON345_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR3072084.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILYLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVIHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6513..8009)
                     /gene="ravA"
                     /locus_tag="ON345_002024"
     CDS             complement(6513..8009)
                     /gene="ravA"
                     /locus_tag="ON345_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR3072085.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8232..10100
                     /gene="kup"
                     /locus_tag="ON345_002025"
     CDS             8232..10100
                     /gene="kup"
                     /locus_tag="ON345_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR3072086.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10267..10686
                     /gene="rbsD"
                     /locus_tag="ON345_002026"
     CDS             10267..10686
                     /gene="rbsD"
                     /locus_tag="ON345_002026"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR3072087.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQVVIRSGECSPYANIILCAGVTF"
     gene            10694..12199
                     /gene="rbsA"
                     /locus_tag="ON345_002027"
     CDS             10694..12199
                     /gene="rbsA"
                     /locus_tag="ON345_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR3072088.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12204..13169
                     /gene="rbsC"
                     /locus_tag="ON345_002028"
     CDS             12204..13169
                     /gene="rbsC"
                     /locus_tag="ON345_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR3072089.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13194..14084
                     /gene="rbsB"
                     /locus_tag="ON345_002029"
     CDS             13194..14084
                     /gene="rbsB"
                     /locus_tag="ON345_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HCR3072090.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14210..15139
                     /gene="rbsK"
                     /locus_tag="ON345_002030"
     CDS             14210..15139
                     /gene="rbsK"
                     /locus_tag="ON345_002030"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR3072091.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15143..16135
                     /gene="rbsR"
                     /locus_tag="ON345_002031"
     CDS             15143..16135
                     /gene="rbsR"
                     /locus_tag="ON345_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR3072092.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITVKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16101..17528)
                     /gene="mdtD"
                     /locus_tag="ON345_002032"
     CDS             complement(16101..17528)
                     /gene="mdtD"
                     /locus_tag="ON345_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR3072093.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17551..18243)
                     /locus_tag="ON345_002033"
     CDS             complement(17551..18243)
                     /locus_tag="ON345_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR3072094.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4448 a         4579 c         4825 g         4617 t
ORIGIN      
        1 agatgacgtt cgccatccag ggctggaacc tgaagtttaa ccactgattc gacctgatat
       61 tcttcgggca acaaagcgat ttcatcttcc ggcatttgcc ctttcagcgc gtagaaacgg
      121 ccttgctcac caggaagatg gtggcaccag ctcaccatat cgttcagaga ggcaaaagcg
      181 cggctaatta cgccatcaaa tggcggctct gaaggaaact cttctaccct gctctgtact
      241 ggttcaatat tctccagttt aagctcatgt tgcacctgac gaaggaaacg cacgcgttta
      301 ccaaggctat ccaacagagt gaaatgggct tcaggacgca cgatagagag tggaatgcct
      361 ggcagtcctg gtccggtgcc gacatcgata aaccgttcac cttgcagata cggtgccacc
      421 acaatgctat cgagaatatg gcgtaccagc atctcattag gatcgcggac cgaagtcagg
      481 ttgtacgctt tgttccattt atgcagcata ttcacgtagg caataagctg gtttttctgg
      541 tgatcggtaa gcgaaatacc tgcgtctttc agcagtaagg agagtttgtt gagcacggtg
      601 attacctgtt cttgatgcgt tgcctggtaa gcgggtgctt accaggcatt tttaatgcgt
      661 tatgcgctac gacgcagcat accctgtttt ttcagccaca ccagcagaat ggagatggcc
      721 gcaggcgtga cgccagaaat acgcgaagct tggccgatag aggctggttt gtgatcgtta
      781 agtttggcga tcacttcgtt agaaagaccg gatacctggc ggtaatccag tgtcgcgggt
      841 agcagggtgt tctcgttacg cagctgcttt tcgatctcat cttgctggcg cgcgatataa
      901 ccttcgtatt taacctgaat ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg
      961 gcaaacggcg tcagcgtggt taatttttca taagtcattt ccggacgacg cagcagatct
     1021 tcaccactgg cttcacggga aagcggcgca gtcaggtgag cattcacttc ggctgcagct
     1081 tccgccgacg gggttaccca ggtcgatttc agacgctgac gctcacgctc gatattctca
     1141 agtttctcgt taaagcgcgc ccaacgttcg tcatccacca ggcccagttc acgaccgatt
     1201 tcagtcaaac gcagatccgc attatcttcg cgtagcatca gacgatattc tgcgcgcgaa
     1261 gtaaacatac gatacggttc tttggttcct aaagtgcaca ggtcatcaac tagtacgccg
     1321 agatacgcct gagaacgtgc cggagcccaa ccttctttat cagcagacag acgggcagcg
     1381 ttaagaccgg ccagcaaacc ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc
     1441 tgaccagcaa agaacagccc ctggataaac ttgctctcca gcgtcggttt caggtcgcga
     1501 ggatcgaaga agtcatactc aatggcataa cccggacgca cgatcttcgc gttttccatc
     1561 ccctgcatag agcggacgat ttgcatctgc acatcgaacg gcaggctggt ggagataccg
     1621 ttcggataaa tttcattaga ggtcagtcct tccggttcaa ggaagatctg atgctgattt
     1681 ctgtcggcga agcgcatgac tttgtcttcg atcgacgggc agtagcgtgg gccgacacct
     1741 tcgatcaccc ctgcatacat cgggctacga tcgaggttac tgcggatcac atcatgggtt
     1801 ttctcgttgg tatgagtgat ataacacggc acctgctggg gatgctggga cgcattgccc
     1861 ataaacgaga ataccggcat tgggttatcg ccatgctgtt gcgccagcac actaaagtcg
     1921 atagttcgag catcaatacg cggtggtgtc ccggttttca gacgaccaac gcgcagcggc
     1981 agttcacgca aacggcgaga aagcggaatg gacggcggat caccagcacg gccaccgctg
     2041 taattatcca gaccgatatg aattttaccg tcgaggaacg tcccaacggt gagcacgacg
     2101 gctttggcac ggaacttcag tcccatttgg gtaacagcac cgaccacgcg atcgttttcg
     2161 acaataagat cttcaaccgc ctgctggaag atcatcaggt tcggttggtt ctccagcgcc
     2221 gtacgtaccg cctgacggta gagcacacga tccgcctgag ctcgggtagc gcgaaccgct
     2281 ggtcctttgc ttgcgtttag tatcctaaac tggatacccg cttgatcgat cgctttcgcc
     2341 atcagaccgc cgagtgcatc cacttctttt accagatgtc ccttcccaat accgccgatc
     2401 gccgggttgc agctcatctg ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt
     2461 tgacccatac gcgccgcggc catcgcggcc tcggtgcctg catgaccccc gccaatgatg
     2521 atgacgtcaa aaggatccgg ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa
     2581 tggattgaag cccgggccgt ggattctact caactttgtc ggcttgagaa agacctggga
     2641 tcctgggtat taaaaagaag atctatttat ttagagatct gttctattgt gatctcttat
     2701 taggatcgca ctgccctgtg gataacaagg atccggcttt taagatcaac aacctggaaa
     2761 ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat aagctgggat cagaatgagg
     2821 ggttatacac aactcaaaaa ctgaacaacc gttgttcctt ggataactac cggttgatcc
     2881 aagcttcctg acagagttat ccacagtaga tcgcacgatc tgtatactta tttgagtaaa
     2941 ttaatccacg atcccagcca ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg
     3001 ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat ctttgagttc tgcctcgagt
     3061 ttatcgatag ccccacaaaa ggtgtcatat tcacgactac caataccgat tgcgccaaag
     3121 cggaccgcag aaagatcggg cttctgttcc tgcaatgctt catagaaagg agaaaggttg
     3181 tccggaatat ctccggcacc gtgggtggag ctgataatca gccagatccc cgaggcagat
     3241 aaatcttcca acagcggacc gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt
     3301 tcagccaggt gttctgctac atattcggca ccgccgaggg tgctgccgct gataagagtg
     3361 atatctgcca taaaccgcca cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt
     3421 gggatctacc tgtggaaaag tatgggatta aaaaagccga ttatggcttg atggtacgca
     3481 tgatcgggtt ttgcaggacg atcaatgtct cggtggactg aatttcatca attgtttgga
     3541 tcttgttgat aagtacatgc tggagagcgt cgatcgaacg gcacatcact tttataaaga
     3601 tgctgtagtg gccggttgtg taataggctt cagtgacttc atcaaggctt tccagctttg
     3661 ccagcgcgga agggtagtct ttggcgctct ttaatataat gccgataaag cagcctacgt
     3721 cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct
     3781 tcattttctc tactcgaacg tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt
     3841 cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag gatgccacgg tccagattgt
     3901 cgatcagata attttccata ggattttctt atgcagattg atgattcatt ctattttagc
     3961 cttctttttt aatgaatcaa aagtgtgtta ggctttttat tgaatgatta ttgcatgtgt
     4021 gtcggttttt gttgcttaat cataagcaac aggacgcagg agtataaaaa atgaaaaccg
     4081 cttacattgc caaacaacgc caaattagct tcgtgaaatc tcacttttct cgccaactgg
     4141 aagaacgtct ggggctgatc gaagtccagg cgccgattct tagccgtgtg ggggatggca
     4201 cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt aaaagtgaaa gctctgcctg
     4261 atgcccagtt cgaagtggtt cattcactgg cgaagtggaa acgtcagacc ttagggcaac
     4321 acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa agcccttcgc cccgatgaag
     4381 accgtctttc tccgttgcac tcggtctatg ttgaccagtg ggactgggaa cgcgtaatgg
     4441 gcgacggtga gcgtcaattc tcgactctga aaagcacggt agaggcgatc tgggcgggaa
     4501 ttaaagcaac cgaagctgcg gttagcgaag agtttggcct ggtaccgttc ctgccggatc
     4561 agatccactt cgtacacagc caggagttac tgtctcgtta tccggatctc gatgccaaag
     4621 ggcgtgagcg gacgatagcg aaagatcttg gcgcggtatt ccttgtcggg attggcggca
     4681 agctgagcga tggtcatcgc cacgacgtgc gcgcaccgga ttatgatgac tggagcaccc
     4741 cgtcagagct gggtcatgcg ggtctgaacg gcgatattct ggtgtggaac cctgtactgg
     4801 aagatgcgtt tgagctttct tccatgggga tccgcgtgga tgccgacacg ttgaagcatc
     4861 agttggcgct gaccggtgat gaagatcgtc tgcagctgga atggcatcag gcgctgctgc
     4921 gcggtgaaat gccgcagacc atcggcggcg gtatcggcca gtctcgtttg accatgctgc
     4981 tgctgcaact gccgcatatc ggccaggttc agtgtggtgt atggccagct gctgttcgcg
     5041 agaacgtccc ttctctgctg taataattta tcgccgccag cgtctgagca ggcggcttcg
     5101 catcccggta tcaaagcgcc agatatgatc gaaaatgcgc atgatgccgg gtttgccgtg
     5161 tgccgacatc gccacggcat gaaagcgatg ctgatgtacc cgctgcagct ctttcacttt
     5221 actcgtcacg tcgtcaggca accgctgagc gataaaatca gaaatcacca ccgcatcggc
     5281 atcaaaccat tccctgcttt gcaagcgttc cataatggcg cgaaaacaac tggcaagatc
     5341 ggtgccgcca cgaaactgct ggcttaaaaa acggattgct tgttcgatgc cttgtgggcc
     5401 tgaaagctca taacggacga tctcggtgga aaatagcata atatagcagc gccggttttc
     5461 agcgagagca atgcgcatca aggccaggca gaacgctttc gcacactgtt cattaaagcc
     5521 gcccattgag ccggaagtat ccacacagac aataaacggc ccgcgcggct gttcatcgta
     5581 atctttatgt atcaccggac gttcgatcac tttttcacgc cacgactcac cgtgcaggcg
     5641 ataggtgagc aactgttttt ccaccagccg acggtaaaac tcatactcca gttccgttat
     5701 ccctagtgtc gccagttctg gcggcagaag acgtaaaata tcatcgcttt gttgcagacc
     5761 atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg gtgcggaagg tttccatctg
     5821 cgcatcgttg cgcggtattg atttggcttc ccgagaacgc cccagctgct ctgccaggcg
     5881 tttcagttcc ggctgttcgt taagaaattc accgtatttc acaatcaact gatagtcgcc
     5941 acgtttaagc tgaccggcgc tcatatccca cagacgacca gctgcagtat tgttatctgc
     6001 gagaatcggt tcaagttgtc cgctcagcgt catgcgttcc tgaacttcac tcaacagttg
     6061 ttcgcgttct tcttctaata gctgttgatt taacgtcgtt gcttgcacga tcagacttaa
     6121 acgccaacgc tggagaaaaa gcgtgtgtaa cgctgaagtg atcgtgctgt tagcatcaac
     6181 caactgtcgg gcttgttctg cccatggaga attcagacga tgcagtaagt acaggatctg
     6241 tggtagctgc acaataaact gtggcgtgga gaggagctgg ctttgctgat agcacatcac
     6301 ctcttcggtg agttccggcg ggactcgggc atctttcagc cgactgcgca gcgcttcacg
     6361 ccagcgggga acatcatcag tgattgccgc cttcaggcgt gggaattttt caaagaagac
     6421 cgccagctgc ggtgaggcca gcagtgcgat gatcatctct tcgatcaatc cctcttcgct
     6481 gacggccagc atcacattaa gcgtatccag cgttagcatt gttgtgcctg gcgaatctgt
     6541 gcaccgacat cctgtaggct ggcttcaatg cgacctaacc agtcgccagg gataaacaga
     6601 catttttgct gttcgctgaa taaagcgtgt tgcttgcgcc agtcgctttc aagctcttcc
     6661 agttgttgtt ttatttcacc aggcagacct tcagccgatg aaccgggaag tgccagcgta
     6721 ctgccttgta aacttacatc gcgaacgaca agatgttggg tgctatcaac ttccagattc
     6781 agtttctggg caaagccgat accgttcagt ttaccgcgaa tttcaccacc tttgctcagc
     6841 cactgttcca gcgcgctacg ctcaaaggag atatgaacca cttccatatc atgcagtttt
     6901 aacggttttt gcagcagtag agtcagagtg gaagcagtaa cgttaacagg aagttgatac
     6961 tgctgacgac ggctgaaaat gccgcccaga cgaattaccg ttaaggctgt tttatcgctt
     7021 tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc caaggcgagt caacatccct
     7081 tgctgttgcc aggcgtgacc ggtcatcaat acatcaattt gttgttgtat caaattcagg
     7141 ctttgcgcgt cataccacag gcaatctttc agcaaaatga gatctaccgg ggcaacagca
     7201 ctgcgaccgc taaaaaaggc gctggcctgc aataatcgga tcgctttttt ccagcgacga
     7261 tccgagacat aaggcgcatc cggtaattta tccagttgct ggcgcagcat aaaaatcagc
     7321 tcaaatacat gatcgggcag cgtaatttca ccaatctctt tctgccagcg ttcatattct
     7381 tcatctgtga cctgcaaggc atcaggaacc ggattgtcgt tttcatcctg ttgactggtc
     7441 agcatggagc ggaaattcgc tttatcctgc actttatcta accacagacg aatcagcatg
     7501 cggtcatata acgcttccag actgctgtct gcttccggca gctcgttgga ggccgccacc
     7561 agcagacgca tcgggatttt ttctacgtgt gcaccgttgc ggaactggcg ctcgttaatg
     7621 gcggtgagca aggtattaag aattgccggg cccgctttcc agatctcatc cagaaagaca
     7681 atttctgctt ccggcaggta accgctggtt aaacgttcat agcgcccttc atcttttagc
     7741 gcctgaatag aaaggggacc aaaaacttct tccggcgtgg agaagcgggt catcagatat
     7801 tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc gggcgatcaa acttttggca
     7861 atacctggcg ggccaaggag gaacacactt tcaccactta atgccgctaa taaacacaag
     7921 cggatggcgt ggctacgttc ataaagcccc ttttccaacg aactgctcag gcgggaaatt
     7981 ctttccgcta ataaatgagg gtgagccata atgaagtggc gtcctttcgt caaaagttct
     8041 gcgtaaattg cgagtataga cgtttcttgc tggtggctaa aatagtctca aagggggggt
     8101 atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg ccgttaatcg
     8161 tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat atttcaacga
     8221 aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc ctcgcggcga
     8281 ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt gaatgtttgt
     8341 ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta tcgctgatct
     8401 tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg cgggcagata
     8461 acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc
     8521 gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc tatggtgaag
     8581 tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa atcgtcgccc
     8641 cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta ttatttatga
     8701 ttcaaaaaca tggcacggct atggtcggta agctgtttgc gccgatcatg ctgacctggt
     8761 ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa gtgctgcatg
     8821 cgctgaatcc aatgtgggcg gtgcatttct tccttgaata caaaacggtt tcttttattg
     8881 cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct gatatggggc
     8941 actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct tccttgaccc
     9001 ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt aagaacccgt
     9061 tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc gcactggcga
     9121 cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt caggcggtac
     9181 gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag tcagggcaaa
     9241 tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg attgtcagct
     9301 ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga accatggtgc
     9361 tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat aagtattttg
     9421 ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc gctaacctcg
     9481 ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg tttatcgtga
     9541 tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa catggtaact
     9601 ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg
     9661 cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat aaccttaaac
     9721 ataacaaggt attgcatgag cgggtgattc tgttaactct gcgtaccgaa gacgcgccat
     9781 atgtccataa tgtccgtcgg gtacagattg aacaactgtc gcccactttc tggcgcgtgg
     9841 tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac cgctgcggtc
     9901 tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat gagtcgttga
     9961 tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac
    10021 gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt atcgaactgg
    10081 gtactcaggt cgaaatctaa cgccagacgc ctcctttctc cataaggggg cgtttttgtt
    10141 ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt aacgtcacga
    10201 tggttttccc aactcagtca ggattaaact gcgggtcagc gaaacgtttc gctgatggag
    10261 aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat ctcccgtctg
    10321 ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa aagtacaacg
    10381 cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct gggcgtcgtc
    10441 acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca acataatccg
    10501 caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca gggaaatacc
    10561 attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggtg
    10621 gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg
    10681 ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag ccttcccggg
    10741 cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgcgtga tggcgctggt
    10801 gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta tctatgctcg
    10861 cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc cgaaatcttc
    10921 ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc agttgaccat
    10981 tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa
    11041 aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
    11101 caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
    11161 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
    11221 aaccgaatcc ctgttccgcg tcatccgcga actaaaatcg caaggccgcg gtattgtcta
    11281 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
    11341 cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
    11401 gatggtgggt cgcaagctgg aagatcaata tccgcacctg gacaaagcgc cgggagatat
    11461 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
    11521 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
    11581 agtgctctac ggcgcactac cgcgcaccag cggttacgtc accctggatg ggcatgaagt
    11641 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
    11701 taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
    11761 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
    11821 tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa ttggtctgct
    11881 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac gccccaaagt
    11941 gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
    12001 actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat cggagatgcc
    12061 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
    12121 atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg gcaagcttaa
    12181 tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc cgttatttca
    12241 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
    12301 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
    12361 cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatcg
    12421 acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
    12481 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc gcaattggtg
    12541 cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct acgctggtta
    12601 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg
    12661 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
    12721 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
    12781 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
    12841 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
    12901 tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg gggactggct
    12961 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
    13021 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
    13081 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
    13141 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
    13201 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
    13261 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
    13321 cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg gtggtgctgg
    13381 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
    13441 ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct gtgaagatgg
    13501 ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
    13561 tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat tacatcgcga
    13621 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt acatccgcag
    13681 cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt aatgttcttg
    13741 ccagccagcc agcagatttt gatcgcacta aaggtttgaa cgtaatgcag aacctgttga
    13801 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
    13861 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
    13921 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
    13981 atcagattgg cgcgaaaggc gtcgaaaccg cagataaagt gctgaaaggc gagaaagttc
    14041 aggctaagta tccggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
    14101 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
    14161 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
    14221 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
    14281 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
    14341 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
    14401 gggtgatgac agcattggtg agagcgttcg ccagcagctc gccactgata acattgatat
    14461 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
    14521 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgcccttt ccccggcgct
    14581 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagca ttattaatgc agctggaatc
    14641 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
    14701 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
    14761 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
    14821 tgcagcgaag gcggcgcagg tactgcatga aaaaggtatc cgtactgtac tgattacttt
    14881 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
    14941 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
    15001 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
    15061 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
    15121 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgcc tggcgggcgt
    15181 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
    15241 cgtcaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagccc tggcgcgtag
    15301 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
    15361 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
    15421 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
    15481 aaaacgcgtt gatggcttgc tgttactgtg caccgaaacg catcaacctt cgcgtgaaat
    15541 catgcaacgt tatccgacag tgcctactgt gatgatggac tgggctccgt tcgatggcga
    15601 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
    15661 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
    15721 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
    15781 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
    15841 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
    15901 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
    15961 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
    16021 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
    16081 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg cttagattta
    16141 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
    16201 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
    16261 gtgccttcca tcccttcata aacgcgaagg acggccgcac ttacagcaac gcctaaacta
    16321 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
    16381 agatcggcaa gtgtgatggt attcatcgcg gtaaattgcg tcgacatagc catccctaat
    16441 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
    16501 gcgatcatta gcccaataat caccgtgatc cccactaacg tatggcgata gcccagacga
    16561 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
    16621 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
    16681 aacggtacac aaccggtccc cagacgggtt gcaatattgc ctacgatacc gattgagaaa
    16741 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtgtgtcg cgcatggaga
    16801 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
    16861 acaatctttt ccccgaatag ctctattccg cttgagaaga gaacaaggct gaggccaaac
    16921 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
    16981 cgcgcgtaaa gaaggcccgc aatacctatg gggatattga ttaaaaatat ccagtgccag
    17041 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
    17101 ggcatggcga caaaattcaa tactggaaga agttcattac gaggataagc gcgcagtaag
    17161 gccagccgag caacaggcat catcattgcg ccgcctatcc cctgaataac ccggaagaca
    17221 accagctgtg gtagcgaatt agaaagtgcg caggccagag aacccaatgt gaacagactc
    17281 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
    17341 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
    17401 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
    17461 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg ccatactgcg cttcttttta
    17521 tcgctcattc aatgtcctgt tatcgggttg tcacttatca ggtgagcgta gcagcgcctg
    17581 acaagcttta aatgccgcgt cgccatcgct ttggacaatc gcatcgacaa tcgcctggtg
    17641 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
    17701 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
    17761 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
    17821 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgcagc
    17881 cagtaggcaa gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
    17941 atgaaagttc tcttctgtca tccaccaggt aagcaattcc tgatcaagaa aattccagtt
    18001 tgattgtggc atgacccggg taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
    18061 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
    18121 aatctcacca ggcaaaatgg tgccgggttc atattcacct tttaagatgc gttgcgccag
    18181 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
    18241 cattactaat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
    18301 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacgggggt tttgccgaaa
    18361 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
    18421 aatgcgccac cactgacacg gaacaacggc aaacacgccg ccgggtcag
//
LOCUS       DAJSKJ010000067        18129 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000067 DAJSKJ010000000
VERSION     DAJSKJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.067"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(160..1188)
                     /gene="epmB"
                     /locus_tag="ON345_002034"
     CDS             complement(160..1188)
                     /gene="epmB"
                     /locus_tag="ON345_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR3072095.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLCQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1230..1796
                     /gene="efp"
                     /locus_tag="ON345_002035"
     CDS             1230..1796
                     /gene="efp"
                     /locus_tag="ON345_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3072096.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1848..1973
                     /gene="ecnA"
                     /locus_tag="ON345_002036"
     CDS             1848..1973
                     /gene="ecnA"
                     /locus_tag="ON345_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR3072097.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2084..2230
                     /gene="ecnB"
                     /locus_tag="ON345_002037"
     CDS             2084..2230
                     /gene="ecnB"
                     /locus_tag="ON345_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR3072098.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2406..2723
                     /gene="sugE"
                     /locus_tag="ON345_002038"
     CDS             2406..2723
                     /gene="sugE"
                     /locus_tag="ON345_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR3072099.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2720..3253)
                     /gene="blc"
                     /locus_tag="ON345_002039"
     CDS             complement(2720..3253)
                     /gene="blc"
                     /locus_tag="ON345_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR3072100.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3342..4475)
                     /locus_tag="ON345_002040"
     CDS             complement(3342..4475)
                     /locus_tag="ON345_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR3072101.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGSDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4538..4897)
                     /gene="frdD"
                     /locus_tag="ON345_002041"
     CDS             complement(4538..4897)
                     /gene="frdD"
                     /locus_tag="ON345_002041"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR3072102.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(4908..5303)
                     /gene="frdC"
                     /locus_tag="ON345_002042"
     CDS             complement(4908..5303)
                     /gene="frdC"
                     /locus_tag="ON345_002042"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR3072103.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5314..6048)
                     /gene="frdB"
                     /locus_tag="ON345_002043"
     CDS             complement(5314..6048)
                     /gene="frdB"
                     /locus_tag="ON345_002043"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR3072104.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6041..7849)
                     /gene="frdA"
                     /locus_tag="ON345_002044"
     CDS             complement(6041..7849)
                     /gene="frdA"
                     /locus_tag="ON345_002044"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR3072105.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8174..9151
                     /gene="epmA"
                     /locus_tag="ON345_002045"
     CDS             8174..9151
                     /gene="epmA"
                     /locus_tag="ON345_002045"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR3072106.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9370..10872
                     /gene="yjeM"
                     /locus_tag="ON345_002046"
     CDS             9370..10872
                     /gene="yjeM"
                     /locus_tag="ON345_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR3072107.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            10924..11238
                     /locus_tag="ON345_002047"
     CDS             10924..11238
                     /locus_tag="ON345_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072108.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            11235..11549
                     /locus_tag="ON345_002048"
     CDS             11235..11549
                     /locus_tag="ON345_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HCR3072109.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11578..14901)
                     /gene="mscM"
                     /locus_tag="ON345_002049"
     CDS             complement(11578..14901)
                     /gene="mscM"
                     /locus_tag="ON345_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR3072110.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14923..15891)
                     /gene="psd"
                     /locus_tag="ON345_002050"
     CDS             complement(14923..15891)
                     /gene="psd"
                     /locus_tag="ON345_002050"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR3072111.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15988..17040)
                     /gene="rsgA"
                     /locus_tag="ON345_002051"
     CDS             complement(15988..17040)
                     /gene="rsgA"
                     /locus_tag="ON345_002051"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR3072112.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17135..17680
                     /gene="orn"
                     /locus_tag="ON345_002052"
     CDS             17135..17680
                     /gene="orn"
                     /locus_tag="ON345_002052"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR3072113.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17891..17966
                     /locus_tag="ON345_002053"
     tRNA            17891..17966
                     /locus_tag="ON345_002053"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17924..17926,aa:Gly,seq:gcc)
BASE COUNT         4183 a         4660 c         4783 g         4503 t
ORIGIN      
        1 aaggttatgc aacgtgcaaa gattaaattt cccagtttaa tgacaggttg ctgtacttgc
       61 gtttgttgct cttgccggat gcggcgtaaa cgccttatcc ggcctacagt tcattgaaaa
      121 tccattcaat tttaatgttt aacttccctg tttaatcagt tactgctggc gtagctggag
      181 atccagcggc gttttgctgg gttcgccgcc gatttctcgc gccagtttcg gcaccagata
      241 acccgacacc aatgtcagca actcacgcat aatctgccgt gcttcgtcat cactcaccat
      301 aaaatgcgcc gcgccctgta ctttatctag cacatgcagg taatacggca tcacgccggc
      361 atcgaacaac gcattactca ggtttgccaa cgtttgtgcg ttatcgttca caccacgtaa
      421 cagaacgctc tggttaagca gggtgacacc gacacggcgc aatttagcca tcgcctgacg
      481 gaacgtttca tctacctcat tggcatgatt gatgtgattc accagcaaga tttgcagcgt
      541 agaacgggca aagcgttcaa ccagcgcctc ggtgatacgt gccgggatca caatcggcag
      601 acggctgtga atccgcagac gtttgatatg cgggatggct tccagttgtg tgagcaacca
      661 gtccagctcg tgatctttcg ccatcagcgg atcgccgccg gagaaaatca tctcgtccag
      721 ttccggatgc gcagcaacat actcaagagc agtctgccag ttacgcttgt tgccctgatt
      781 ttcggcatag gggaagtggc gacggaagca atagcggcaa tttaccgcgc agccgccttt
      841 gaccagcaaa agcgcccggt tgtggtattt atgcaacaaa ccaggcacta cgctgtgctg
      901 ttcttccagc gggtcggtgg agaatccgga cgcgacgaca aactcatctt gcgaggtaag
      961 tacctgacac aaaagaggat cgtccggatt gcctttctcc atgcgatcga taaatgagcg
     1021 gggcacacgc agggcaaaca gctttttggc gctgcgtccg gccaacagtt tttcgtccgc
     1081 gtctatattc aaaagacgca gaagttcatc aggatcggtc acaacatcgg caagttgcgt
     1141 taaccaatct tctctggatg gggtatttag ggttacaata tgcgccattt tgtggcttag
     1201 ctaccaatta acaaatttca gagggcctta tggcaacgta ctatagcaac gattttcgtg
     1261 ctggtcttaa aatcatgtta gacggcgaac cttacgcggt tgaagcgagt gaattcgtaa
     1321 aaccgggtaa aggccaggca tttgctcgcg ttaaactgcg tcgtctgctg accggtactc
     1381 gcgtagaaaa aaccttcaaa tctactgatt ccgctgaagg cgctgatgtt gtcgatatga
     1441 acctgactta cctgtacaac gacggtgagt tctggcactt catgaacaac gaaactttcg
     1501 agcagctgtc tgctgatgca aaagcaattg gcgataacgc taaatggctg ctggatcagg
     1561 cagagtgtat cgtaactctg tggaatggtc agccgatctc cgttactccg ccgaacttcg
     1621 ttgaactgga aatcgttgat accgatccgg gcctgaaagg tgataccgca ggtactggcg
     1681 gcaaaccggc taccctgtct actggcgctg tggttaaggt tccgctgttc gtacaaatcg
     1741 gcgaagtcat caaagtggat acccgctctg gtgaatacgt ctctcgcgtg aagtaatgcg
     1801 gttgtggtgc ggcctgcagg ctgcaccatc acttattcag gtcagagatg atgaaacgcc
     1861 ttatcgttct tgttttgctt gccagcacgc tgctcacggg ctgtaacacc gctcgcggtt
     1921 tcggcgaaga catcaaacat ctcggcaact ccatctctcg cgctgccagc taatttttct
     1981 tctcttccga aaaatcatca gatttccgtc atttttggcg atgttgtcta ttattaattt
     2041 gctataggca aacataaata acattaccta aaaggaagac gttatggtga agaagacaat
     2101 tgcagcgatc ttttctgttc tggtgctttc aacagtatta actgcctgca acaccacgcg
     2161 tggcgttggt gaagacattt ctgatggcgg taacgcgatt tctggcgcag caacgaaagc
     2221 gcagcaataa gcaataacgg tacgacagca gtgtcgtgcc gtttgttttt tctgcgatag
     2281 tcacaaaggt aatagttgaa attcccctgc cacctggcaa aatatccgtt caaccatcag
     2341 ctttgcagga cgacctgcaa acgcctcttt tcaccgggga cggccccaaa tttccggagc
     2401 ctgatatgtc ctggattatc ttagttattg ctggtctgct ggaagtggta tgggccgttg
     2461 gcctgaaata tacccacggc tttagtcgtt tgacgccaag tgttattact gtgacggcga
     2521 tgatagtcag tatggcgcta cttgcctggg cgatgaaatc gttaccggta gggacggctt
     2581 atgccgtgtg gacgggtatt ggcgcagtcg gcgcggccat caccggcatt gtgctgctcg
     2641 gtgagtccgc taacccgatg cgcctggcga gtctggcgtt aatcgtattg gggattattg
     2701 gtctgaaact cagcactcac taactaccgg gctgctgtac ccaaataaac ttactgacat
     2761 caaacccttc ccgggtcgcg actgccagca tctcctgttt cacttcgtca gaaatggttg
     2821 gcgtgcggga gagtatccac aggtagtcgc ggtccgggcc gcaaaccagc gcatggcggt
     2881 attcccgatc aagtgcaatg acgttataac cgccatagaa aggaccaaag aatgacactt
     2941 tcagcgcagc gcgtgttggt gcgccggtaa agtacgcttt cccttcactc tgctgccaca
     3001 tttctctgtc agggttatag cctttattga tgacattcag gccgccgtca tcacgcagac
     3061 tgtatgttgc ggtgactttt tccagtccac gttcaaaacg gtgatcaaaa cgggcaatct
     3121 cataccaggt accgagatag cgtttagcgt cgaaattatt cactactgtc acgccacgcg
     3181 gcggcgtagg agaactgcag gcaacgacca gaaatgcagc tgtcgctgcg gcaacgagag
     3241 ggagcaggcg cataaatgtt tccttactgg tttttttcta agtgtagatg acagcaagga
     3301 aaagcgcaga aaaggtccga aaattcggac ccgatgaaat tttactgtag agcgttgaga
     3361 atctgccagg cggcggcgac tctcgctgga ttgggatagt ttttgttagc cagcatcact
     3421 atacccagct ctttttctgg aataaacgcg acatagctac caaatccgcc ggttgcccct
     3481 gttttatgta cccatgatgc gcgtactgca ggagttgggg gcgtaatcgc ttttacgggg
     3541 tgtgctgcca gtgcaatttt attgccactg ccgttaatga tgctgtcagg atttaccggc
     3601 cagtccagca tttcccagcc caggccctga tacatatcgc cggtttgcca gtagcgagat
     3661 tgtgccagtt gtatcccttg ctgaagtgtt ttgtcgttga tatcacgggg attcatattg
     3721 cttcgtaccc agcaggccat atcttcaatg gtcgacttca caccataagc ttcagcatct
     3781 aacgcccctg gcgaaacatg cactgcctta ccttcgcgat atccccaggc gtaattcttt
     3841 tcttctgggg gaggtacatt aatccacgta tggttgagtt tgagtggctg gaagacacga
     3901 gtttgcatcg cctgctcaaa actcaaacca gacggcttca cagccagtgc gccgaacaaa
     3961 ccgatactgg agttggcata cagacgttgt gttcctggag cccatgcagg ctgccagttt
     4021 tgatagaagc gcagcaagtc gcttgaggat ttcacttcat ccggcacctg caatggcagg
     4081 ccgccagcgg tgtaggtcgc aagatgtaat agtgtgatcc cattccactg tttagcggta
     4141 agttcaggcc agtattttgt tgcgggatcg cttaacttga tttcccctcg agcaatagcg
     4201 tcgctaccaa gcacgcccgt aaatgttttg ctgaccgaac ctaactcaaa caacgtttgc
     4261 tgtgtgacgg gctgcttttt ggcgatgtcc gcatagcccc aggtaaagta ataaggttta
     4321 ccctgataaa ttaccgccac cgccataccg gggatctttt gttgctctat aagcggggta
     4381 attgtgcgat gcacaatatc gttgatttgt tgaggggcag caaatgtgga gcaagaggcg
     4441 gtaattaata aggcgcagag cgtcgttttg aacataaggt ctggtttcca tacaaaacgg
     4501 cccgccatag gcgggccgga tttacattgg cgatacgtta gattgtaacg ataccaatca
     4561 gcgtgacaac tgtcaggata gcagccagac cgtagaaaac ccatttgccc gcaggcacgt
     4621 gaattttcag atcgtgcatc gcgtggtgca tacggtgtaa accacaccac agcggcagaa
     4681 cgatcatcag gaacaggaat acgcgaccaa tgaagctctg cgcgaacgcc agaacgcgct
     4741 cgtagctcag cgcatcaccc ggaaacagcc ccagtggcag cagaataccc accagcagga
     4801 tcatcaccgg cgcaataatt gcgctccaca taccaccggc cccgaagagg ccccagaata
     4861 ccggttcgtc agaacgcttt ggatttggat taatcatctc aggctcctta ccagtacagg
     4921 gcaacaaaca ggattacgat ggtggcaacc acagttaccg cccagagact tttgataatt
     4981 ggctctggtc ccattttttc gtcttttaca atgatattgg ccgctttcgg tgccagttca
     5041 aaccaggttt tggtgtgcag cagagctgcc gccagagtga tcaggttaat gatcacgata
     5101 accgggtttt gtaaaaagtc gacgaatccc gcccaggctt ccgggccatt tttcagggca
     5161 aacagcccga aaatcagttc aatgctgaac cacacagccg gaaccgccgt gccttcgcgc
     5221 agcatgtaaa agcgataaaa cggcaatttt ttccaccagg tggacgtcat tggccgtaca
     5281 tacggtttac gtttagtcgt catgttgcac tccttagcgt ggtttcaggg tcgcgataag
     5341 aaagtctttc gaactttcta ctttgccctg ctgaatggcc gcagccggat cgacgtgttt
     5401 cgggcagact tcggagcaat agcccacgaa agtacagctc catacgccgt tctggctgtt
     5461 caactgcgcc atacgctcct tcttaccgtg gtcgcggcta tcttcgttat aacgatgcgc
     5521 cagcgtaatg gcagccggac cgatgaactc tgggttcagg ccaaactgcg ggcacgcggc
     5581 gtagcacaga ccacagttga tgcaaccgga gaactggtga tactttgcca tctgcgccgg
     5641 ggtctggatg ttagtaccct gatccgcggt gcgggagttg ccgatgatgt acggtttgat
     5701 cgcttccaga ctttcgatga agtgggtcat atcgaccacc agatcgcgtt caatcgggaa
     5761 gttagctaac gcttcaacct tcataccgtc ggtgtaatca cgcaggaagg ttttacatgc
     5821 cagttttggc acgttgttaa ccatcatgcc gcaggaacca caaatcgcca tacggcagga
     5881 ccagcggtaa ctcaggtccg gtgccaggtt gtctttgatg tagcccagcg catccagtaa
     5941 tgaggtagtt gcgtcataag gcacttcata gaatgcgcta tgcggtgcgg tatcgacttc
     6001 cgggttatag cgcaccacct caattttcag gtttttcatc tcagccattc gccttctcct
     6061 tcttattggc tgcttccgcc ttatcggctg catccgcttc gccaccgtaa acgcgtttag
     6121 ctggcggcag cgtagtaatc ttcacgtcgc tgtactccag gcgagtcgtg ccatcagcat
     6181 cgcggaaggc gagggtgtgt ttgaggaagt tgacgtcgtc acgctcggtg caaccttcgt
     6241 ccagacgctg gtgcgcgccg cgggactctt tacgtgccat tgcggagtgc gccatacatt
     6301 cagcaacgtt cagaccgtgg cccagttcaa tggtgtagag caggtcggtg ttgaacacgc
     6361 tggaagtgtc ggtgatgcgc acgcgcttga agcgttcctg cagctcagcc agcttgtcga
     6421 tggttttctg catcagttcc ggcgtacggt agataccgca gccttcttcc attgccaggc
     6481 ccatttcgtc gcggatcttc gcccagtttt cgccaccatc ctggttaacc agatctttca
     6541 gacgttgttc aacgccagct gcctgcgctt caattgccgc ttcgttgcca ttaccggcag
     6601 ttgctgcacg ctctgtcgct tgttcaccgg ccagacggcc gaagaccacc agttccgcca
     6661 gggagttgga gcccagacgg tttgcaccgt gcagaccaac agaggaacat tcacccacgg
     6721 cgaacagacc tttaatgcgg gtttcacagt tctgatcggt ttcgataccg cccatggtgt
     6781 agtgtgcggt cggacgtacc ggaatcggtt ctttaaccgg atcgacgcca acgtacgctt
     6841 tcgccagttc gcagatgaac ggcagacgtt catgcagttt tttctcgccg aggtgacgca
     6901 agtcgagata aaccacatcg ccacgcggcg tggagatggt gttgccttta cgccattcgt
     6961 gccagaaggc ctgagaaact ttgtcgcgtg gacccagttc catatatttg tttttcggct
     7021 cgcccagcgg agtttccggg cccatgccgt aatcttgcag ataacggtag ccatttttgt
     7081 tgaccagaat accgccttca ccgcggcagc cttcggtcat caggataccg gaacctggca
     7141 gaccggttgg gtgatactga acgaattcca tgtcacgcag cggaacgccg tggcttagcg
     7201 ccatacccat accgtcaccg gtaacgatgc cgccgttggt gttgtaacga taaacgcgac
     7261 ccgcaccgcc agtagccata acgaccgcgt tagcacggat ctgcaccagc gtgccttcca
     7321 tcatgttcat tgctaccagg ccgcgaacat gaccatcatc aaccagaata tccagcacga
     7381 aatgttcgtc aaaacgctgg atctgcggga attgcagaga ggtctggaac agcgtgtgca
     7441 gcatatggaa gccggtctta tcggcggcga accaggtgcg ctcgattttc atgccgccga
     7501 agcgacgtac gttgacgcta ccatccgggc gacggctcca tgggcacccc cacagttcca
     7561 gttgggtcat ttcggttggg cagtggtgga cgaaataatc cacgacatcc tgctcacaca
     7621 accagtcgcc acccgctact gtatcgtgaa agtgatattc gaagctgtca tgatcctgcg
     7681 cgacagcggc ggagccccct tctgcagcaa cggtatggct acgcatcggg tatacttttg
     7741 agattagtgc gatttttgca tttggatttg cctgcgcggc agcaattgca gcacgtaatc
     7801 ccgcgccacc ggcgcctaca atggcaagat cggcttgaaa ggtttgcacg acattcctcc
     7861 agattgtttt tatcccacag ccccgtactt cagggtaagt gcctgaaagt tacggtctgc
     7921 gaacgctatt ccactgctcc tttataggta caacagtata gtctgatgga taagtctgaa
     7981 atttgacgag atcgattttt ttagtgcgca agagggctaa attatcactg aagatgatta
     8041 atttaattac taaaccatca gatcgtgctt tttttagtca ctgcccaccg ctgtttgatt
     8101 cctgcgtaaa atttgtctcg ccgtcgcgtt gcgagtagac ttcgtgccct tgtcaaaaac
     8161 tggagattta actatgagcg aaacggcatc ctggcagccg agcgcatcca ttcctaactt
     8221 attaaaacgc gcggcgatta tggcggagat ccgtcgtttc tttgccgatc gtggagtgct
     8281 ggaggtggag acgccttgta tgagccaggc gacggtaacc gatattcatt tggtcccgtt
     8341 tgagacacgt ttcgttggcc ccgggcattc gcaggggatg aatctctggt taatgaccag
     8401 cccggaatac catatgaaac gcctgctggt tgccggttgt gggccggtat tccagctgtg
     8461 ccgcagcttc cgtaatgaag agatggggcg ttatcacaac cctgagttca ctatgctgga
     8521 gtggtatcga ccgcactatg atatgtaccg gttgatgaac gaggtggacg atctcttaca
     8581 acaggtgctg gactgcccgg cagcagaaag cctttcttat caacaagctt tcttgcgtta
     8641 tctggaaatt gacccgctct ctgccgacaa aacgcaactg cgggaagtcg cagcgaaact
     8701 ggatttgagt aacgttgctg ataccgaaga agaccgcgac acgttgctac aattgctgtt
     8761 tacctttggc gtagagccaa atattggtaa agaaaaaccg acctttgtgt accactttcc
     8821 agccagccag gcatcactgg cgcaaatcag taccgaagat catcgggtcg ctgaacgctt
     8881 tgaggtttat tataaaggta ttgagctggc gaatggtttc catgaattaa cggatgcccg
     8941 tgagcagcaa caacgctttg aacaagataa ccgtaagcgc gcggcgcgcg gtttgccgca
     9001 gcaccccatt gaccagaatc tgattgaagc cttgaaagtc ggtatgcctg actgttccgg
     9061 cgtggcatta ggtgttgatc gtctggtgat gttggcgctg ggcgcggaga cactggctga
     9121 agtcatcgcc tttagcgttg accgggcata attctgaaaa attactgaat taacagcgaa
     9181 gaatggcgtg ataagcgcca ttctgcagca tatgttttca tttttccgtt aaaggccaca
     9241 atttaagact ggatatttga tatcatccag gtatcaattc tgtattggtt tttacgctgc
     9301 ctgctcaatg ttgcgcagta cagttcagtg ggcacgtgtt atacacgcgc tgaaatgaag
     9361 gatggtttca tgcctcacac gataaaaaag atgagtctga taggactcat attgatgatc
     9421 tttacttccg tatttggatt tgccaatagc ccatcggctt attacttaat gggttatagt
     9481 gcgattccct tttatatatt ttctgcattg ttattcttta ttccattcgc cttaatgatg
     9541 gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg gatgaataat
     9601 agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc ttatatcatc
     9661 tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct ctatggtagc
     9721 gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt tggtctgctg
     9781 gcagtggcat ggatgattct ggtcaccgtc gttgcttcaa aggggattaa taaaattgcc
     9841 cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt gctgttagta
     9901 agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa tttccttgca
     9961 tcaccgaacc caggttatca gtccggtctg gcaatgctat cgtttgtggt atttgctatt
    10021 tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga aaatccagaa
    10081 aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg ttattcgctg
    10141 gcaatatttt tatggggtgt cagcacaaac tggcagcagg tattaagtaa tggttccgtt
    10201 aacctcggca atattaccta tgtgctgatg aagagcctcg gggtgacgct gggtaacgca
    10261 ctgcatttgt cacctgaagc gtcattgtcg ctgggtgtat ggtttgcgcg tattaccgga
    10321 ctttcgatgt tcctcgctta taccggggcg ttctttacgc tttgctattc accgctgaaa
    10381 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
    10441 atggggatgc cttctatcgc catgtggatg cagtgcgggt tggttactgt cttcatcctg
    10501 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
    10561 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ctttaaagca
    10621 cgtcaggatc tcgacagacc gtttgtgatt ttcaaaacgc atttgtcggc aatgattgcg
    10681 acagtggttg tcgtactggt ggtgacattt gcgaacgtct tcaccatcat tcaacctgtg
    10741 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
    10801 ctgttagcga tggcgattta ccagaactat tgcagcagag tggcaaaaaa tccgcagtgg
    10861 gcggtggaat aacccgatgc cctatcgtct acctgacgat agggcgtaaa gacaggaaat
    10921 gttatggatg acaccagccg ggacccggca ataaccgaag atgaaatcag ggcgctgcaa
    10981 ttttctgccg gggatgtagc ggaaatagag cagacgattc tctcttttgt cgatgcctgc
    11041 cacacccgta aagtggcgat ggtggtgggt agcaccatta acaccctcaa agatcgcgat
    11101 ggtaaacggt ggggaaacct gccggatatt tattgcgctt atcttatccg atgtctggtt
    11161 tttcgcgggg aattagtggg atacggcgat ttgtttcgta tgcgctatag cgaaattaag
    11221 cgaccggtta ccttatgagt gcgcgcatgt ttgtcttatg ctgcatctgg tttattgtgg
    11281 cgttcctctg gataaccatc acttccgcgc tggacaaaga gtggatgatt gatggacgcg
    11341 ggattaacaa cgtctgcgat gtgttgatgt atctcgaaga agacgatacc cgtgatgttg
    11401 gtgtgataat gacgttaccg ctctttttcc cttttctctg gttcgctttg tggcgaaaaa
    11461 aacgcggctg gtttatgtac gccaccgcgc tggctatttt cggctactgg ctgtggcagt
    11521 tttttctgcg ctatcagttt tgtttgtgag ccggattggt tcatccggca cacaaactta
    11581 caaacttccc gcctgacgac ctttgcccgc agacgtcagc gttctccccg tttgtttacc
    11641 gttgaggctt tccagacgca tctggaaggg cgggaatggc atatcgatac cgtgggcatg
    11701 gaagccagcc agaatcagct ggtggatctc atggcgtagc ggcatacggt gacccatctc
    11761 agcggcgtaa atacgcagct cgaaaatctg aatcccctgt tgcagatcca ccaggaagac
    11821 ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc gctgcggtga gcaggatttc
    11881 cgtcacttct tcgctattgg catcggcagg ggccggtatc gtcaacacca cgcgcgtgac
    11941 cgagtcagag agcgaccagt tgataaactg ctcggtaata aacgccttgt tcggcacgat
    12001 tatctcttta cggtcccagt cgctgatggt ggtggcgcgg gtgttaattt tcgtcacgct
    12061 accggtgaga tcgcgaattg tcaccgtatc gccaatgcga atcggttttt cgaacaggat
    12121 aatcagacca gagataaagt tggcgaaaat ttcctgcaaa ccaaaaccga gaccaacacc
    12181 gagcgcggca accagccact gcaatttcga ccactcaata ccaatcattg agaagccgac
    12241 cagcccgcca atcagcatca gcagatattt ggtgatggtg gtgatggcgt aacccgtacc
    12301 cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc agcgcgggca ggttgcgcac
    12361 cagctgcgtg gtgatgataa ataccagaat ggcaattagc actgcaccga gggtaatcgg
    12421 ctccaggctt tctacgccct gtacagtgga agtgacatcc cacagagaaa tattttcgag
    12481 gaagccgaaa gcggaatgga tttctgacca cagcacaatg accgaaagca gggcgatcaa
    12541 catcaaaatt gagcgcacca gccgcaagga ttgcgcactg atggcatcga gatcgacttc
    12601 gctttcatcg acttcaattg ccccttccgg gctactgtga tgatgcgctt cctcttcgcc
    12661 acgcgcgcgt tgcgctaaca tctctgcccg gcgatgcttc gcccggtcaa acgccagcct
    12721 gcgacgctgg atcagcatcc agcggcggat aacgtgataa accaccagta gcaggaacca
    12781 gatggcaacc gaggtttcaa gcctcgctaa cagcgcctgt gccgttgcca gataacccac
    12841 agccgacgcc agaatggcaa ccaacggcgc gccaatcatc atgttccaca gcatatggtt
    12901 ggtaatgttg tcgccgctgc cctctttgtt gagatacagt gggatcccgg cctttttcag
    12961 gctgagggtg accaccgcca gcgcaccgca aatgaggata aagcaaagcc gtcccagcga
    13021 accggagaat tctcggtcgt cgaggttatc gaacatcatc agcgccataa tcagcggcac
    13081 aataagcccg atgctcatca ggtagtagcg catcccacgg gaaacacgtt cgcgcggcca
    13141 gccaaaatga gcgataaaca agccgttcgg gcgggcaaag gtggcgcaaa tcatcactac
    13201 ccacagcagc ggcacggtgg ccgttacacc atcgccaatc gcgaccgcca gcggataagg
    13261 ccacgcctcg cgcaagccgt aacccagcgt catccacagc accggtaacg gtgacgcgac
    13321 gagaatcgac cagaaaagag tgcgcaacgt cagccaaaag tgatcctgag tcactttgcc
    13381 aactttcgcc gccgaacgtt caagaaaacg ggtgaaatag cggcgcgagt aaatactgca
    13441 accgaccaga atcaacgcgc caaacagcgg caaaattgtc tctttgctgg tcagcatcat
    13501 cacactggct ttgcccaact gactgaaggt gtccagcgaa atgagacgac gcagatcctg
    13561 ggcgatttcc agcggccagg cgatggtcat cgggcgcacg tcagaggtcc agaacagata
    13621 gcggtgcgtt gcttcgttca cctctttcag cgcatcctcc agttgcccgt tggagacttt
    13681 cagcttggtc agttccaata gtagcgtgtc gccaccctgc aacaatgagt tcagcaactc
    13741 acgctgagtg cgcagttgtg cttccagaat acggttttgc tcggcagtca gcggctgacc
    13801 gtcggcctga tgaatttgcc gtagcagcgg ctgtttatta agcagatcct cataacgtaa
    13861 ccgttgcaca cgcaactgcg ccatttcggt atcaagctgt tgtggtttcg gcatttccgg
    13921 cagccgtgcc acctgcgccc gcagcgcttc gccgagcaga ttggacgatc ccagccattg
    13981 cgactgttca cgcagcgtat tcaacgcctg ccggacctgt aacgtctggc tggcagcctg
    14041 acgctgttgc gaggcaacga gatccatccg ctgcgcctgt tgattcaaag ccgccgatag
    14101 ttcgcggtta attttgaatt gcgcgacgat atctttcggc aaatcggcgc tgttttctgc
    14161 cagcaattcg gtactttcca gcgcccgctc cgcctcaagc tgacgttggc tgtttaattg
    14221 attacgcaag gcctgcaaat acgcatccag ttgctggctc tctttttccg ccagctctga
    14281 gcgtaagcgc gctaattcct ggcggttatt ggcagacagc tgcgccagct ccagttcatc
    14341 aacgagcgcc ttaagacgtg cagagtcaga ctgcaacgcg aaattttgtg cctgattgag
    14401 cggagtattg ccggtaagcg ttcccaggcg gcgctcgatc tcatttaact gacggcgggc
    14461 gtcggtttgc tgttgcggca gttgattcag cgaatcggca atctcgcggg cgcgctcctg
    14521 ctcttgctgg gcctgacggc ttttatccag caactggctg ctgacctgga gaatttcctg
    14581 attcagcgcg tcggtagaca ttcccggcga cacgctgcgc ggctcgtcac gcatgttgtt
    14641 taattgtgcg cgcagagtag cggagagttt cggataatta tcgataactt gctgatattg
    14701 tttgatgcgc tcaagggaac cttttcgttc ctcaagcgca tttaaggcag actggagcgc
    14761 ctctacgact tccggctgtg cgggtttcgc cgcttttgcc tgctccagtt cctgagtgat
    14821 ttgtttgcta tcgggggccg tcgcggcgta cgccccccaa ctgaggcacc aggccatcag
    14881 aaaagtgata atcaggcgca cgtcagcgtt tcctttgatg gattagacct ggtctttttt
    14941 atcgtcaacc aatgggctgg cgtcgtgttc tgcttcgatc tcttcagcag gaagtggggc
    15001 gggttcagcg tctggcgtaa caaaggtttc ggtagatact gccagcggct ggccaatttt
    15061 cgtgacagac aggctttcca gttgctcaac cagattcact ttacccggtg caaacaggtt
    15121 gataacggtg gaaccgagtt taaagcgacc catttcctgg cctttcagca gtgccacaga
    15181 accgtcgttt tccccggcag gccaggtcca gcgcttgatg ataccttcgc gcggcggcgt
    15241 aatggtgccc gcccagaccg tctcaatgct gccaacaatc gtcgctccga ccagaatctg
    15301 cgccattggg ccaaattcgg tatcgaaaag gcaaatcacg cgttcgttac gggcaaacag
    15361 attcggcacg ttctgagcgg tgagatggtt aacggagaag agatcgcccg gcacgtagat
    15421 catctcacgc agaataccgt tgcacggcat gtgtacgcga tggtagtcac gcggggagag
    15481 gtaagtggtc acaaacgtac cgttgcggaa caggtccgcc atcagatagt tgcctgccag
    15541 cagggcttcg aggctgtagt tgtggccttt ggcttgcagg attttatctt cttcgatttt
    15601 acccaactgg ctgataacgc catcggcagg catgaccagt acattcggat cggtatcgat
    15661 tgggcgtact tcgtcacgca gcggacggac aaagaattcg ttaaaggtgc ggtagctggc
    15721 ggtgtccggc ttttgcgcct ctttcatgtc gaccttgtag tatttaacga acagatcgat
    15781 aaccagtttt gtcagccatc ctgcccgctt gcttgcgccc caacccgcca ggcgagtaag
    15841 ccatagtttc ggcagaatgt actgtagcga aagtttaaat gaatttaaca aggtagcctc
    15901 caggccattg ttttgtcgtt cctgatccgg ccaacatgcc ggatcctgaa aaaaagggga
    15961 cgattctaac gacggttagc ttaattgtca gtcatccgta tcagaaaagt ttttacgcgt
    16021 ttttacctgc gccatgcttt ccagaatacg gtgatagttt tcgaaacggg tttccgcgat
    16081 tttcccttcc tcaaccgctt cccggatagc gcagcccgga tcggtatcgt gtttgcaatc
    16141 gcgatattta cacagaccta aatagtcatg gaattcgaca aagccctgag tgatttgttc
    16201 cggctccagg tgccagaggc cgaactcacg cacaccaggg gaatcaatca catcaccacc
    16261 gtgcgggaag tgatacagcc gagcggcggt cgtggtgtgc tgaccaagcc ccgagttgtc
    16321 agagacatcg ttggtcagga tctctttttg cagccccagc aacgcgttca gcaggctgga
    16381 tttgccgacg ccagactgcc cggcaaaaat gctgatgcgc ccggtcaacg cctcttccag
    16441 cggttttagc ccatcctgag tatggctgga aaccatcaat acgcgataac cgatattgcg
    16501 gtagatatcc atctgctcgt tgacgaacgc catgccttcg tcgtccagca ggtctatctt
    16561 gttgagcaca ataatcggct caatctgcaa ggtttcgcag gccaccaggt aacggtcgat
    16621 aatattgagc gacagctccg gcaaaatggc ggagacaatg acaatctggt cgatgttggc
    16681 ggcaataggt tttacgccgt cgtagaaatc cggacgcgtc aacaccgagg tacgctcatg
    16741 caccgcttcc acgatccctt tgacatttac gccttccgcc gccggtttac ccggacgcca
    16801 gactacgcgg tcgccggtta ccagcgaacg gatggtacgg cgaatattgc agcggtgaac
    16861 gtcgccatcg gcggattcca catcagcgtg cataccaaag cggctgatga cgataccttc
    16921 atcaggctcg ccaaacagat tgtcgtcgta gtcgggcttc tccttagacg ttttaagacg
    16981 acgctggtga ttggcgttca cgcggcgctg ctggcctttg gagagtttat ttttactcaa
    17041 tcgtacagac tcctggtcgc ccctgatggg caaaacatct atgatacacg caattgtgga
    17101 tcaatatagt cactgtgaat gggtggaaaa tagcatgagt gccaatgaaa acaacctgat
    17161 ttggatcgat cttgagatga ccggtctgga tcccgagcgc gatcgcatta ttgagattgc
    17221 cacgctggtg accgatgcca acctgaatat tctggcagaa gggccgacca ttgcagtaca
    17281 tcagtccgat gaacagctgg cgctgatgga tgactggaac gtgcgcaccc ataccgccag
    17341 cgggctggta gagcgcgtga aagcgagcac gatgggcgat cgggaagctg aactggcaac
    17401 gctcgaattc ttaaaacagt gggtgcctgc gggaaaatcg ccgatttgcg gtaacagcat
    17461 cggacaggac cgtcgtttcc tgtttaaata catgccggag ctggaagcct acttccacta
    17521 ccgttatctc gatgtcagca ccctgaaaga gctggcgcgc cgctggaagc cggaaattct
    17581 ggatggtttt accaagcagg ggacgcatca ggcgatggat gatatccgtg aatcggtggc
    17641 ggagctggct tactaccgcg agcattttat caagctgtaa aatttttcct ggtcacgtaa
    17701 gcgccggaat ccacttgcgg cggcgtgatt ttgacgctaa attgaacgtc ttgctgataa
    17761 tttcagcact tgagataaaa acgcaaaaaa aacttttttg gggggttgca gagggaaaga
    17821 tttctcgtat aatgcgcctc ccgtaacgac gcagaaatgc gaaaattacg aaagcaaaat
    17881 taagtagtac gcgggaatag ctcagttggt agagcacgac cttgccaagg tcggggtcgc
    17941 gagttcgagt ctcgtttccc gctccaaaat ttgaaaagtg ctgcaaagca cagaccaccc
    18001 aatcgcagta tttcgaattg cgacaaggcg gcaactgaac gaatccttgg gaacttacaa
    18061 aagtatgtga ctaaggtgag tgaaggcgcc aaggcagtag cggttcgaaa gacgaagatt
    18121 aagcgggaa
//
LOCUS       DAJSKJ010000068        18020 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000068 DAJSKJ010000000
VERSION     DAJSKJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.068"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..543
                     /locus_tag="ON345_002054"
                     /pseudo
     CDS             1..543
                     /locus_tag="ON345_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            646..1149
                     /locus_tag="ON345_002055"
     CDS             646..1149
                     /locus_tag="ON345_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR3072114.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1216..2673)
                     /gene="nuoN"
                     /locus_tag="ON345_002056"
     CDS             complement(1216..2673)
                     /gene="nuoN"
                     /locus_tag="ON345_002056"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR3072115.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2680..4209)
                     /gene="nuoM"
                     /locus_tag="ON345_002057"
     CDS             complement(2680..4209)
                     /gene="nuoM"
                     /locus_tag="ON345_002057"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR3072116.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4373..6214)
                     /gene="nuoL"
                     /locus_tag="ON345_002058"
     CDS             complement(4373..6214)
                     /gene="nuoL"
                     /locus_tag="ON345_002058"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR3072117.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6211..6513)
                     /gene="nuoK"
                     /locus_tag="ON345_002059"
     CDS             complement(6211..6513)
                     /gene="nuoK"
                     /locus_tag="ON345_002059"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR3072118.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6510..7064)
                     /gene="nuoJ"
                     /locus_tag="ON345_002060"
     CDS             complement(6510..7064)
                     /gene="nuoJ"
                     /locus_tag="ON345_002060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR3072119.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQDIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7076..7618)
                     /gene="nuoI"
                     /locus_tag="ON345_002061"
     CDS             complement(7076..7618)
                     /gene="nuoI"
                     /locus_tag="ON345_002061"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR3072120.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7633..8610)
                     /gene="nuoH"
                     /locus_tag="ON345_002062"
     CDS             complement(7633..8610)
                     /gene="nuoH"
                     /locus_tag="ON345_002062"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR3072121.1"
                     /translation="MSWITPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8607..11333)
                     /gene="nuoG"
                     /locus_tag="ON345_002063"
     CDS             complement(8607..11333)
                     /gene="nuoG"
                     /locus_tag="ON345_002063"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR3072122.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAVAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPTVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11386..12723)
                     /gene="nuoF"
                     /locus_tag="ON345_002064"
     CDS             complement(11386..12723)
                     /gene="nuoF"
                     /locus_tag="ON345_002064"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR3072123.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12720..13220)
                     /gene="nuoE"
                     /locus_tag="ON345_002065"
     CDS             complement(12720..13220)
                     /gene="nuoE"
                     /locus_tag="ON345_002065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR3072124.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13223..15025)
                     /gene="nuoC"
                     /locus_tag="ON345_002066"
     CDS             complement(13223..15025)
                     /gene="nuoC"
                     /locus_tag="ON345_002066"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR3072125.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILKQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15119..15781)
                     /gene="nuoB"
                     /locus_tag="ON345_002067"
     CDS             complement(15119..15781)
                     /gene="nuoB"
                     /locus_tag="ON345_002067"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR3072126.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15797..16240)
                     /gene="nuoA"
                     /locus_tag="ON345_002068"
     CDS             complement(15797..16240)
                     /gene="nuoA"
                     /locus_tag="ON345_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR3072127.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16871..17809)
                     /gene="lrhA"
                     /locus_tag="ON345_002069"
     CDS             complement(16871..17809)
                     /gene="lrhA"
                     /locus_tag="ON345_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR3072128.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADVPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4488 a         5024 c         4662 g         3846 t
ORIGIN      
        1 cgccccgaca cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg
       61 tgcatccacg taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag
      121 agttctttgt cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac
      181 gactctgcgc ctggtagcca ggattaccct tacccgcaat gtcctggctt tatcccgatc
      241 ggggggactt tattgcctgg tcggcagaat acaggatatc aacgccgttc gtcaggtaaa
      301 aacggcattg ttatcatccc gggatttatc tgtttattct atgaatgccc catggtttat
      361 tcccggtatt gatttctctg accacctgaa ttattggcaa cacgatattc ccgccgtaat
      421 gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg atacagcaga
      481 cagattgaat tatcagaaaa tggctcagat ggtgttataa ccttgttaca caacagtaaa
      541 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
      601 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggt
      661 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
      721 ttgatggata atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc
      781 tgggattttt tacactttgg gctgacaggc acctctgctt ttgatcccgc gaagaatgat
      841 ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag atggcataga tggcttcctg
      901 ggattgacct ggaatcagga gttagccgcg acaatcgacc gactggaatc actggatcga
      961 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1021 gtgacctttt cagaagagtt agaggggcaa ctctttgctt cgatcatgct tgatatggaa
     1081 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1141 gttgggtgat ggtgtcaggg caccataaaa aaccgccgaa catgtcggcg gctttctgac
     1201 ttacaaactg atagcttaca tcagcggcat tgctaaccgc acaatgctaa tcagcggttg
     1261 tggccataca cccagcacca gtaccaacag tgcagagatc agcaccacga taccgcctgc
     1321 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggggcgt gcaggtacag
     1381 gctcaccgcc acgcgcaggt agtagtaaag gccgattgcc gaaccgacaa ccacggcgcc
     1441 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccaataaa
     1501 gcccagcgtc atcggaatac cggccagaga cagcatcatc accgtcatca ccgccgcgag
     1561 gatcgggcga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     1621 gtacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     1681 ggccaggtaa actcctaccg cttccatcga catctcgccc gtttgcagcg caatcagcgc
     1741 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatattggt
     1801 ctggctcagc gccatcaggt taccgaagat gatagaggca aaggcgataa tcgccagcac
     1861 tacccgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     1921 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     1981 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc caaggccaac
     2041 aatcatcagg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2101 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2161 cagcaggaaa gaagacgctg cggcagaaag gatagtgtat ttgatactgg cttccagcga
     2221 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcgat
     2281 accgaggaac agagacgcca gatggttggc attcgccagc aggatcccgc ccagcgcggc
     2341 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2401 agcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     2461 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     2521 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     2581 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     2641 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     2701 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     2761 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     2821 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     2881 agcgcgatgt aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     2941 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3001 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3061 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3121 ataaagctga ccacaaagaa taaacagacc cgccgccgac aaaccgtgcg caatcatctg
     3181 gattaccgcg ccctggtagg ccaactggct gccggtgtag atagcaatca gcacgaagcc
     3241 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctggg cgaaggccat
     3301 ccacgcaccg tagaagatgc cgataacacc cagccacata gcaattggcg cgaactctgc
     3361 cgacgcgttc gggaacagcg gcagggagaa acgcagcaaa ccgtaagcgg cagttttcag
     3421 caagatcccc gcgaggtcaa cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     3481 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     3541 taacaggtat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     3601 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcaa tcaacatcac
     3661 caggccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     3721 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     3781 ccagaagaag aagaacaaga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     3841 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     3901 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagcccgtc
     3961 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4021 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4081 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4141 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4201 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4261 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcacc gcgaaggcgg
     4321 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4381 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4441 gcagatagcc gttctcactt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     4501 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     4561 gcctgacgaa cactttgtcg tacagccagt caaagcccca ggcgttgtac caccaggtac
     4621 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagtacgt ttacccagcc
     4681 acagccaggc tgccagcaga atgccgacaa tcgcgaccac gccagaggta atttccaggg
     4741 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     4801 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     4861 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     4921 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     4981 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5041 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5101 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5161 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5221 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5281 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5341 gcagcgtaac cgccccgaca atgcccacca gatgcagaat ttccggcgtc atcaggaaca
     5401 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catggttgcg gcgtggatca
     5461 gcgcggagac aggcgtcggg cccgccatcg cgtcggcaag ccatgtctgc aacggcaact
     5521 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     5581 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     5641 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     5701 cgaacgcttt cattgccgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     5761 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     5821 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     5881 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     5941 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6001 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6061 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6121 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6181 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6241 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6301 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6361 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6421 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     6481 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     6541 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     6601 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     6661 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat atcctgatca
     6721 ttaacaccaa ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccgga
     6781 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     6841 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     6901 tccagcgcac cggcgaagta agcgcccagt gagaagaaca ccccggaaat cgccagcagc
     6961 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7021 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7081 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7141 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7201 agatcagcag atcctctttt tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7261 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7321 tgcagcgtga gaagttgatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7381 ctttttgcag cgagatacag ccgaccgggc aggctaccgc gcagaggtta caggctacgc
     7441 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     7501 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     7561 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     7621 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     7681 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acctggtcat aacgcggacg
     7741 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     7801 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     7861 tgcagagatg gtcacaatcc cgatgtattc acccacgaag aacagaccga acttcatgcc
     7921 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     7981 gtgacggtga cataccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8041 gataacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8101 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8161 agaagcacgc atcgcaccga gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8221 caccgcgtaa actgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8281 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8341 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8401 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     8461 gttaggtccg taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     8521 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     8581 ctcaatcagt tccggtgtta tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     8641 gcgccagcca gcaccggagc aatgccagac atacccatcg gcaagcccac ctgccctgcc
     8701 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     8761 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     8821 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     8881 aggtggtagt acggcgcgat acgccatttc ccgtcctgtg gctggaagcg tgccggtacg
     8941 ctggtgaagt aatccagacc attttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9001 aagcgcagtt taccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9061 cccggtgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9121 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9181 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9241 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ctgccagttc cgggattttc
     9301 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9361 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9421 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
     9481 ccgtcgcttt cagcaaagct ggcagcggaa agcaccagat gggcgttttc catgatcgct
     9541 gttcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
     9601 gtagcagaag cgtgacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
     9661 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
     9721 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
     9781 ttcgcgacgt tagccgccgc ctgaatcacc tctatgctac cggcgttcgt cccggagata
     9841 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
     9901 tcaggttcga taccgtcaac cgttggtgcg gagttatcca gcgcatgggc gatggcaaaa
     9961 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10021 cgggtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttaaga
    10081 attgccgcaa tctgccagtc agccactttc tgtgctaccg ccatttcgcg cgctttacct
    10141 ttcaccgcct gacgcactgc cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10201 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10261 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10321 tagaagtttt cttcgcccac cagttcacgc agtgcaaagt tgctttccac gctggcacgc
    10381 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10441 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    10501 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    10561 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    10621 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    10681 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggttgg gcaaatttcg
    10741 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    10801 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    10861 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    10921 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    10981 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11041 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11101 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11161 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11221 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11281 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11341 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11401 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    11461 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    11521 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    11581 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    11641 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    11701 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    11761 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    11821 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    11881 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    11941 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12001 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12061 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12121 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12181 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12241 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12301 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12361 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12421 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    12481 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    12541 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    12601 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    12661 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    12721 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    12781 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    12841 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    12901 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    12961 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13021 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13081 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13141 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13201 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13261 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13321 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13381 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13441 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    13501 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    13561 gcatgttgtt gaggcactgc ttaagaatgc gcagactctg gcgcagctct tccactttca
    13621 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    13681 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    13741 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    13801 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    13861 tcggcatcca gtcgaggaac tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg
    13921 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    13981 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14041 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14101 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14161 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14221 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14281 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14341 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14401 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    14461 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    14521 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    14581 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    14641 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    14701 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    14761 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    14821 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    14881 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    14941 gttcgccaat caccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15001 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15061 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15121 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15181 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15241 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15301 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15361 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15421 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    15481 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    15541 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    15601 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    15661 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    15721 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    15781 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    15841 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    15901 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    15961 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16021 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16081 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16141 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16201 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16261 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16321 aaaaaacctt acaatcactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16381 tcctttctat aaccttttgt caactttaac aaaagtttct tcacattagt ttacataata
    16441 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    16501 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    16561 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    16621 tgtcattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    16681 ggtgattttt ttcaggcgat tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa
    16741 atgaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    16801 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    16861 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    16921 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    16981 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17041 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17101 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17161 aagccagtcg ccatggaaca tctgctttat taagcgtcgc cagcaccata tcgcgaaacg
    17221 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17281 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17341 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17401 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    17461 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    17521 gattactgaa cattaatgat gagcaggcct catcattaaa acgcagtatt ttcctggcgt
    17581 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    17641 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    17701 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    17761 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    17821 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    17881 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    17941 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaaataga tcacagaata
    18001 tatcttcagg gatcgcatat
//
LOCUS       DAJSKJ010000069        18011 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000069 DAJSKJ010000000
VERSION     DAJSKJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.069"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(102..1694)
                     /gene="lsrK"
                     /locus_tag="ON345_002070"
     CDS             complement(102..1694)
                     /gene="lsrK"
                     /locus_tag="ON345_002070"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HCR3072129.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAVELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQIIADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1773..2726)
                     /gene="lsrR"
                     /locus_tag="ON345_002071"
     CDS             complement(1773..2726)
                     /gene="lsrR"
                     /locus_tag="ON345_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR3072130.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMITLQRLSGFISSQQIRLVTLSGGVGFYMTGI
                     GQLNAACSVNIIPAPLRASSADIAHTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2975..4510
                     /gene="lsrA"
                     /locus_tag="ON345_002072"
     CDS             2975..4510
                     /gene="lsrA"
                     /locus_tag="ON345_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR3072131.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLIRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERCRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4504..5532
                     /gene="lsrC"
                     /locus_tag="ON345_002073"
     CDS             4504..5532
                     /gene="lsrC"
                     /locus_tag="ON345_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR3072132.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGSGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5532..6524
                     /gene="lsrD"
                     /locus_tag="ON345_002074"
     CDS             5532..6524
                     /gene="lsrD"
                     /locus_tag="ON345_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR3072133.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6536..7558
                     /gene="lsrB"
                     /locus_tag="ON345_002075"
     CDS             6536..7558
                     /gene="lsrB"
                     /locus_tag="ON345_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HCR3072134.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGVQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7585..8460
                     /gene="lsrF"
                     /locus_tag="ON345_002076"
     CDS             7585..8460
                     /gene="lsrF"
                     /locus_tag="ON345_002076"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR3072135.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8484..8774
                     /gene="lsrG"
                     /locus_tag="ON345_002077"
     CDS             8484..8774
                     /gene="lsrG"
                     /locus_tag="ON345_002077"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR3072136.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8831..9589
                     /gene="tam"
                     /locus_tag="ON345_002078"
     CDS             8831..9589
                     /gene="tam"
                     /locus_tag="ON345_002078"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR3072137.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLAESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9593..10507)
                     /locus_tag="ON345_002079"
     CDS             complement(9593..10507)
                     /locus_tag="ON345_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR3072138.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAIIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10714..12165)
                     /gene="uxaB"
                     /locus_tag="ON345_002080"
     CDS             complement(10714..12165)
                     /gene="uxaB"
                     /locus_tag="ON345_002080"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR3072139.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRLLC"
     gene            complement(12392..13812)
                     /locus_tag="ON345_002081"
                     /pseudo
     CDS             complement(12392..13812)
                     /locus_tag="ON345_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(13951..14310)
                     /locus_tag="ON345_002082"
     CDS             complement(13951..14310)
                     /locus_tag="ON345_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR3072140.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGISLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14310..15236)
                     /gene="glsB"
                     /locus_tag="ON345_002083"
     CDS             complement(14310..15236)
                     /gene="glsB"
                     /locus_tag="ON345_002083"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR3072141.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15300..16688)
                     /gene="sad"
                     /locus_tag="ON345_002084"
     CDS             complement(15300..16688)
                     /gene="sad"
                     /locus_tag="ON345_002084"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR3072142.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16789..17670
                     /locus_tag="ON345_002085"
     CDS             16789..17670
                     /locus_tag="ON345_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3072143.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            17748..>18011
                     /gene="yneK"
                     /locus_tag="ON345_002086"
     CDS             17748..>18011
                     /gene="yneK"
                     /locus_tag="ON345_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR3072144.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFK"
BASE COUNT         4508 a         4612 c         4492 g         4399 t
ORIGIN      
        1 caagctggct tttaacaagc cagctctaaa agaagggaaa taagaataac tatactcaaa
       61 aactaacagc cacggtcatc atgatgtggc tgtcaatgaa actataaccc aggcgctttc
      121 cataacgacg tcgtcagtcc atgatcaacc agccccagct gatcctgata aactgcctgc
      181 cacttatcgc gtgaatcctg ataaagttca tgcttttccg ggtctggtgt gtgcgtccgt
      241 tcccagcgaa ccaggcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg
      301 ccagctgcaa tggcacatcc taatgcggtg gcttctttga ctaccggaat attgacgggt
      361 aatcccgaga catcagcgat aatttgactc cataatttcc cttttgaacc tccgccagca
      421 aaaaccagcg aggtgggatg aatattcgag aaatcagcaa tttgctgcaa gttacacgct
      481 gatacaatcg ccgcattttc ttccagcgca cgaaacaatg tcgctttgtt acatttatcc
      541 gggtcaatgg acaagttaat aaaggaaggt gcggcgtgat accaggtttt aaagcgcatt
      601 ctgtcggaga agatcggcat tacgccccac gacccaggcg gcacccgact tgccatctct
      661 tccagcagcg tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc ttcggcacag
      721 aaagcatcgc ggaaccagcg catggtgagt ccggtaaaaa agcttataga ttcagcttgt
      781 accatgccag gaataacatg aggattaacg cgcacgttca tttctgggtc tgtcaccggc
      841 gcggctaaat ttacaacttg ctgccagaat gtgccgccaa gaaccgcggt ttgtgccgga
      901 cgcacaacgc ctaacccaag gcaaccaagc tgcacgtcgc ctcctccaac gaccaccgga
      961 gtgcccgcct tcagaccgca gagttccacc gcttgtgaac ttaccacgcc cagcaatgtg
     1021 ccggtttctt tgacaggaga aagaatatcg gcacgcaggc cagccatatc cagcaatgca
     1081 ggtttccagt cacgggtggt tagatcaaga agtcccgtgg tgccagcgtt agaaggatcc
     1141 accgccagtt cgccgctgag catataggcc agccagtcgc tgatcatggt gatggttgat
     1201 gcctgacggt aaatatcgga acgatggtgc gccagccaaa gtaatctggg gatggcactt
     1261 aaagccagtg tttgtccggt cgcgcgataa acttcgtttt caaaggtatt gttgtgcagt
     1321 tctttaagtt cgctaacttc gcgtgccgct ctggcatcca cattggcgca ggcccagatc
     1381 ggtgttcctt cattgttata taaaacaatg ccttcacgca tagaacatgc cgaaacggca
     1441 gcgatatact ccgggtctat gccggcgttg tgcagcgcct ggcgcataca ctcacacgcc
     1501 agttgccagt ttttgttgag atcaaattcc atagaaccag gaacgtccgg tactgccagg
     1561 tgccgccact ccgcctgtcc cactgctatt tgattgcctt ccaggtcgaa tatcacagcc
     1621 cgaatacttc cggtgcctgc atccagcgcc atcaggtagt actttgattc tgaaggggta
     1681 aagagtcgag ccattagatt atcctcggct tatgcgttct gtctgttcct ctatacgttc
     1741 tccatcattc ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc cgctgctgtg
     1801 tcctgatcag taaccagtgc gttgatataa ccgcctttca ttgcagcggc aattgcttcg
     1861 gctttatttt ctccccctgc cacgccaacc cggacgggta tggtcttcag cgcgcttaaa
     1921 ggtaagccaa tcagttcgtt atgtattttg atatccgtga caacgtcacc ttttgcatca
     1981 aaaaagtagc ctaaaatgtc gccaaccgcc ccttttcggc caatcattaa ctgttcgccc
     2041 tggctgatat aaccggagcg aatgatcgtc gcgtcgtctt gttgactcac ggccccaatg
     2101 ccgacaatcg ccacatccgc tgcttgcgcg gctaacagaa catctttgac gcaattttca
     2161 ttttttagcg tatgggcaat gtcagcggag gatgcccgca acggagccgg aataatattc
     2221 acactgcacg ccgcgttaag ctgcccgata cccgtcatat aaaaaccgac gccaccggag
     2281 agcgtgacca ggcgaatttg ctgtgacgaa ataaaaccac ttaagcgttg cagcgtaatc
     2341 atggttgcct cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa actcatcaac
     2401 atatgcgccg cgcctatccc cagtcgcccg ccgacatcag catccgcaag cccagggatc
     2461 acccggacat gttgcagcga aaactgacga cgtaattgag tttcatattc cagacagcct
     2521 tcaaagcgag aattaatctg tacgcgaata atgccggact gatgcccttt ctccagcaag
     2581 cgcgacactt tcaaacgggt caggccgaga cgatcgctga tctcgctctg ggtcagcccg
     2641 tcgtgatagt aaaaccacgc gatccgcgcg acctgttctt cttcacacat tccctgttct
     2701 gaaattgccg aatcgttgat tgtcataatt cattcttcac tttgaacata tttaaatctt
     2761 taatgcaatt gttcagttct tgcgcattta tatctgtgat ggcaaccaca gtttgactct
     2821 acgagcatga acaaacgcaa ccgtgaaaat caaaatagca taaattgtga tctattcgtc
     2881 ggaaatatgt gcaatgtcca cctaaggtta tgaacaaatt aaaagcagaa atacatttgt
     2941 tcaaaactca cctgcaaaac tgaacggggg aaatatgcaa acgagtgata cccgcgcgtt
     3001 accgctactt tgcgcccgct cggtttataa acagtattca ggggtcaatg tcctgaaagg
     3061 catcgatttt acgttgcatc agggggaggt ccacgccctg ctcggcggca atggtgccgg
     3121 taaatcgacg ttaatgaaga ttattgccgg tattacccct gctgatagcg gtacgctgga
     3181 gattgggggc aacaactacg ccagattaac gccagttcat gctcatcagc tgggcattta
     3241 tctcgttccc caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa acatcctgtt
     3301 tgggatggca aaaaaacagc tctccatgca aaaaatgaag aacttgctgg cggcgctggg
     3361 ctgccagttt gatctgcata gtctggcagg atcgctggat gtcgccgatc gccaaatggt
     3421 ggaaatcctc cgcgggctga tacgcgactc gcggattctg atcctcgatg aacctaccgc
     3481 ctcgcttacc cctgcggaaa ccgaacgctt gtttagtcgc ttgcaagagc tgcttgctac
     3541 tggcgtgggt attgttttta tctcgcataa gctgccggaa attcgccaga ttgccgatcg
     3601 aattagcgtg atgcgcgacg gaaccatcgc cttaagcggc aaaaccagcg aactgtctac
     3661 cgacgacatt attcaggcca tcaccccagc ggtacgggaa aaatcgctct ctgccagcca
     3721 aaaattatgg ctggagttac ctggtaaccg cccacaacat gctgccggaa cgccggtgct
     3781 gacactggaa aatctgaccg gcgaaggttt caggaatgtc agcatgacgc tcaatgccgg
     3841 agaaattctg ggcctggctg ggctggtggg ggccggacgc acagaactgg ccgagacgct
     3901 ctatggtctg cgtactttgc gtggcggacg tattatgctg aatggtaaag agatcaataa
     3961 attatccact ggagaacgtt tactgcgcgg tctggtttat ctgccggaag atcgccagtc
     4021 atccggactg aatctcgatg cttcgctggc ctggaacgtc tgcgccctta ctcataacct
     4081 tcgtggattc tgggcgaaaa ccgcaaaaga taatgccacc ctggaacgtt gtcgtcgggc
     4141 gctgaatatt aaatttaacc aaccggaaca agctgcacgg acattatccg gtggcaacca
     4201 gcaaaaaatc ctcattgcca aatgcctgga agcttcgccg caagtattga ttgtcgatga
     4261 gccgacgcgc ggcgtggatg tctcggcccg taatgatatc taccagctgt tgcgcagcat
     4321 cgccgcacaa aatgtggctg tgctgcttat ctcctctgat ctggaagaga tcgaactgat
     4381 ggcagatcgc gtgtatgtga tgcatcaggg cgaaattgcc cactctgcac tgaccgggcg
     4441 cgatattaat gtcgagacta ttatgcgcgt tgccttcggc gatagtcagc gtcaggaggc
     4501 gtcatgctga agtttattca gaacaaccgt gaaatcacgg cactgctggc agtggtgctg
     4561 ctgtttgtat tacccggttt tctcgaccgc cagtatttaa gtgtgcaaac gctgaccatg
     4621 gtttatagca gcgcgcaaat cctgatcctg ctggcaatgg gcgcgacgct ggtaatgctt
     4681 acgcgcaata ttgatgtttc agtgggttcg attaccggaa tgtgcgcggt gctgttgggg
     4741 atgttactga acgcaggata ttcactacct gttgcctgtg tcgcgacttt actgcttggt
     4801 ttgctcgcgg gatttttcaa cggtgtcctg gtcgcgtggc taaagatccc tgccattgtt
     4861 gccacccttg gcacgttagg gttgtacaga ggcatcatgt tgctgtggac tggcggtaaa
     4921 tggattgaag ggttacccgc cgaactgaaa cagctctccg caccgctgct gtttggcgtt
     4981 tccgcgattg gttggttgac gataattctg gtggcattta tggcctggct gctggcaaag
     5041 acggcgtttg gacgcagttt ttatgccacg ggcgataatt tacagggcgc tcgtcaactg
     5101 ggtgttcgta cggaagccat tcgcattgtg gcattttcgt tgaacggctg catggcggca
     5161 ctggcgggaa ttgtgtttgc ttcgcagatt ggttttatcc ccaaccagac cggttccggg
     5221 ctggagatga aagcaattgc agcctgcgtg ctgggcggca ttagtttgct cggcggttcc
     5281 ggtgcgatca tcggtgcggt actcggcgca tggttcctga cgcagatcga tagcgtactg
     5341 gtgctgttgc gcattccggc atggtggaac gattttatcg cgggcctcgt gctgctggcg
     5401 gtgctggtgt ttgatggacg cctgcgttgt gcgctggaac gtaatctacg gcggcaaaaa
     5461 tatgcccgct ttatgacgcc accgccatcc gttaaacccg cttcgtcagg taaaaaacgg
     5521 gaggccgcat aatgcgtatt cgctacggtt gggaactggc tcttgccgca ctgctcgtta
     5581 ttgagattgt cgcatttggt gcaattaacc cgcgaatgtt agatctcaat atgttgctgt
     5641 tcagcaccag tgactttatc tgcattggca ttgtcgccct accgctgacg atggtgattg
     5701 tcagtggcgg gatcgatatt tcgtttggtt cgaccatcgg cctctgcgcc attgcattgg
     5761 gcgtactgtt tcaaagtggt gtgccgatgc cgctggcgat actcctgacc ttactgctcg
     5821 gcgcattgtg cgggctgatc aacgccggat taattatcta taccaaagtt aacccgctgg
     5881 tgattacgct tggcacgctg tatctgtttg ccggaagcgc tctgctgctt tccggtatgg
     5941 ccggagcgac ggggtacgaa ggtattggtg gattcccgat ggcgtttaca gatttcgcta
     6001 acctggatgt gctgggactc cccgttccgc tgattatctt cctgatatgt ctcctcgttt
     6061 tctggctctg gctgcataaa acccatgccg gacgtaatgt gtttttgatt gggcaaagcc
     6121 cgcgcgtggc gctttatagc gcgattccag ttaaccgcac cttatgtgcg ctctatgcca
     6181 tgacggggct ggcgtctgcg gtcgccgctg tgctgctggt atcgtatttt ggctcagcac
     6241 gttccgatct cggtgcgtcg tttctgatgc ccgccatcac cgccgtggtg cttggcgggg
     6301 ccaatattta tggtggttcc ggttccatta tcggcaccgc cattgcggtt ttattagtgg
     6361 gatatttgca acaaggtttg caaatggcag gagtgccaaa tcaggtgtcc agcgcccttt
     6421 ccggtgcgct acttatcgtc gttgtcgtag gtcgttccgt tagcctgcat cgccagcaaa
     6481 ttaaagagtg gctggcgcgt cgggccaata acccattgcc ataaaggata tcttcatgac
     6541 acttcatcgc tttaagaaaa tcgccttact tagcgctctt ggcattgccg caatctctat
     6601 gaatgtgcag gccgcagagc gtattgcatt tattcccaaa ctggttggcg tgggattttt
     6661 taccagcggt ggcaacggcg tacaacaagc gggtaaagag ctgggcgttg atgtgaccta
     6721 cgacgggccg acagaaccca gtgtttctgg tcaggtacag ttgattaata acttcgtcaa
     6781 tcaaggttat aacgccatta tcgtttctgc ggtttcgcct gatggcttgt gtccggcact
     6841 gaaacgcgcc atgcaacgtg gtgtgagagt gctgacctgg gactctgata ctaaaccgga
     6901 gtgccgctct tactacatta atcagggaac gcccgcccag ttgggaggta tgttggtgga
     6961 tatggcggcg cgtcaggtga ataaagacaa agccaaagtc gcgtttttct actcaagccc
     7021 caccgttacg gaccaaaacc agtgggtgaa agaagcgaaa gcgaaaatcg ccaaagagca
     7081 tcctggctgg gaaattgtca ctacgcagtt tggctataac gatgccacta aatcattaca
     7141 aaccgcagaa ggaatattaa aagcgtatag cgatctcgac gccattatcg cccccgacgc
     7201 caacgccctg cccgctgccg cacaagccgc agaaaacctg aaaaatgaca aagttgcgat
     7261 tgtcggattc agtacgccaa acgtgatgcg tccatatgtg gaacgcggca cggtgaaaga
     7321 atttggcctg tgggatgtgg ttcagcaagg caaaatttca gtgtatgtcg cggatgcatt
     7381 attgaaaaaa ggatcaatga aaacgggcga caagctggat atccagggcg taggtcaggt
     7441 ggaagtctcg ccaaacagcg ttcagggcta tgactacgaa gcggatggta atggcatcgt
     7501 actgttaccg gagcgcgtga tattcaacaa agagaatatc ggcaaatacg atttctgatg
     7561 tgcattactt aaccggagta agttatggca gatttagacg atattaaaga tggtaaagat
     7621 tttcgtaccg atcaaccgca acaaaatatc ccttttatcc tgaaaggttg cggtgcgctg
     7681 gattggggaa tgcagtcacg cttatcgcgg atatttaatc cgaaaacggg taaaaccgtg
     7741 atgctggcct ttgaccatgg ttattttcag gggccgacta ccggacttga acgcattgat
     7801 ataaatatcg ccccgctgtt tgaacatgcc gatgtattaa tgtgtacgcg cggcattttg
     7861 cgcagcgtag ttcccccggc gaccaataag ccggtggtac tgcgggcgtc aggtgcgaac
     7921 tctattctgg cggaattaag taatgaagcc gtggcgttat cgatggatga tgccgtgcgc
     7981 ctgaacagtt gcgcggtggc ggcgcaggtt tatatcggca gcgaatatga acatcagtcg
     8041 atcaaaaata ttattcagct ggtcgatgcc ggaatgaaag tgggaatgcc gaccatggcc
     8101 gtgactggcg tgggcaaaga catggtgcgc gatcagcgtt atttctcgct cgcgactcga
     8161 atcgccgctg aaatgggggc gcaaattatc aaaacctatt atgtcgaaaa aggttttgaa
     8221 cggattgttg ccggatgtcc ggtacccatt gttattgctg gcggtaaaaa attaccggag
     8281 cgcgaggcgc tggaaatgtg ctggcaggct atcgatcagg gcgcttctgg tgtggatatg
     8341 gggcgtaata ttttccagtc tgaccatccg gtggcgatga tgaaagccgt acaggcggtg
     8401 gttcaccata acgaaacggc tgatcgggca tatgaactct atctgagtga aaaacagtaa
     8461 ctgcggattt aaggagaaga attatgcacg tcacactggt tgaaattaac gttcatgaag
     8521 acaaggttga cgagtttatc gaagtttttc gccagaacca cctgggctct gtacaggaag
     8581 aaggtaattt gcgcttcgat gtcttacagg acccggaagt gaattcgcgc ttttatattt
     8641 acgaagccta taaagatgaa gacgcagtgg cattccataa aaccacgcct cactacaaaa
     8701 cctgtgtcgc gaaactggaa tctttaatga ccgggccgcg taaaaaacgt ctgttcaatg
     8761 gtttgatgcc gtgaggcgaa tttataagtt ttatctacaa ttggggtaac gcgctgacgg
     8821 gagtaaataa atgtctgact ggaacccctc tttatatctg cactttgccg ctgaacgatc
     8881 gcgtccggcg gtggagctgc ttgccagagt gccgctggag aatgtcgaat atgttgccga
     8941 tcttggctgt ggcccaggta acagcaccgc cctgctgcat caacgttggc ctgcggccag
     9001 gataacaggc atcgactcgt ctccggcgat gattgctgaa gcgcgcagtg ctttgccaga
     9061 ctgccagttc gtggaagcag acatccgcaa ctggcaaccg gtacaggcac tcgatctgat
     9121 ttttgctaat gcctcactgc aatggctgcc cgaccactac gaattgtttc ctcacttggt
     9181 ttctttactt aatccgcagg gcgtgctggc agtacagatg ccagataact ggctggagcc
     9241 gacccatgtg ctcatgcgcg aagttgcctg ggaacaaaac tacccggatc gcgggcgtga
     9301 gtcgttggct ggcgttcatg cttactacga tattttgagc gaagccggat gtgaggtcga
     9361 tatctggcga accacttact atcaccagat gccgtcgcac caggcgatta tcgattgggt
     9421 gaccgccact ggattacgtc cgtggttaca ggatctggcc gagagcgaac agcagctttt
     9481 tcttacgcgc taccatcaga tgctggaaga gcagtatcca ctgcaagaga acggacagat
     9541 actgctggca tttccgcgtc tgtttattgt tgcccgccgt acggagtaat tatcacgtca
     9601 gctggtaatg acgatcggga agaatttttg ctggaatttc tgcttcatcg ttcatctgta
     9661 acaggtcaat ttcaatagcg ttgcagatgg catccagcgg taaatcattg ttttcagtac
     9721 cgaacggatc ttccagttct tccgccagac aatccagcga aataaaagtg taggaaatca
     9781 gcacagagat aaaaggcgtc atgtaatgca ggtccacgac cagcgcgaac ggcagcatga
     9841 tacaaaacag ataaacggta cgatgcaaaa tcagggtgta ggcaaaggga attggcgtgt
     9901 aggcaatgcg ctcacagcct gccagaaccg ctgaaatatc attaagccga tcgttgaggc
     9961 taataaacag gatatctgaa agctgcccat tgcggcgctg aaccgccaac cactccccca
    10021 ttattaacaa gatacggtta gctggagagt tcgaagccag tacaggctga agatcttcag
    10081 tcttgagata atgagccagc acttccgcct gtggctgttt gcgtaatgtc atgcgtaaac
    10141 agtgggcgaa ggcgatttgc aaccgggcaa actcccttac acttgccgaa tccggcaatg
    10201 tcgtttttac ctcgcgcagt aacgaccgtg aggcaatcat caactgcccc caaagttttc
    10261 gcgcttcaac gtaacgggcg tacccggcat tattacgaaa accaagaaaa atggcgatgg
    10321 cgacaccgag aatgctgaac ggtgcgaggg tgaatttgat gcccagatgc gtgtaccagg
    10381 gcagcatgaa aataatagcg atagaaaaga gaaaattgag tagtaagcgc gatgatatct
    10441 tggataatac tgagccgtgc cagacaaaaa tacggcgcag ccagtgttgt tgtggacgaa
    10501 caatcatggt tatcttcagg cgtgaaaaag tcgccctatt aaacgtgatt acgatcacat
    10561 tctcaagacg ctacttacaa attacctaca agcttacaac attaacaggc gctgcatgtg
    10621 gcatctgtta atgataaagg cacataggat gttgtaacta atatagtgaa ataaaaaacc
    10681 cggtcgaaac cgggttcaga agtaacggtg ttattagcac aacaggcgta cagcttcgcg
    10741 catccctttt tcgagaatcg catccaggtc attagccacc tgctcgacca gaccaggcac
    10801 ttgcgtcagg tcctgctccc agtggtcttt ttctgccaac acaatcgcta ccagttcctg
    10861 ggtaccgata acacggtcac gatgctggct ccagagttgc tgataacgtt ccagccagtg
    10921 tgcatcgtcc tgtaccggat atgtttctcc gttacgctca ccgcgataga acgcaatcaa
    10981 tgccgctaat gcgaaagtaa ggcgcgccgg aagtgtgccg tttgccttct gccctgccag
    11041 cagctgcggc aggatgcggg tgcggaactt ggtcataccg ttgagcgcga tagacagcag
    11101 ctgatgctta atgtacgggt tacggaaacg cccggtgact gcactggcga aagattccag
    11161 ttcatcacga ggcaaatcca gtaccggaat aatttcttcg tagatggctt tttcaacgaa
    11221 tgcgcaaatt tcagcatcgt tcatcgcttc acctacggta tccagccctg cctggaacgc
    11281 caccggcacc agcgcggtgt gcgcaccgtt gaggattgcc actttgcgtt ctttgtacgg
    11341 tttaatatcg tcaacaatca gcacgttgag cggatatttg tccagacgca gttcagtcgc
    11401 taaggatttc ggtccctgaa tcacaaacag gtaaaagtgt tcagcggtgt cgagaaaacc
    11461 atcgtgataa cccagctctt cttccagttt agccacttca tcgcgcggat aaccggtaac
    11521 gatacggtcc accagcgtag aacagaagct gttagcctga tccagccatt gaataaatgc
    11581 ttctggcagt gcccactctt gcgcatagcg cagcaccagt tcacgcaacg cgtcgccatt
    11641 gtagtcgatc aactcacacg ggatgatgat ccaaccttta tccagcgcac cgttgaaatg
    11701 gctaaagcgt tcgaacagca gacgggtcag ttttgccgga tagcttaccg ctggcgcgtc
    11761 atcgaattta tcgcccgcat ggtagctgat acccgcttcg gtggtgttag agaaaacaaa
    11821 gcgcatttcc gggttgtgcg ccagtttcag gaattcatcg tattcactgt agacgctgat
    11881 ttcacgatta accgagcgaa tcagacgcgc gtcgctgacc gcttccccct tctcattcag
    11941 gccacggata atggtggtat acagaccatc ctgcgtgctc agtgacggcg ggaatgaagt
    12001 ttcaatcgga cgaacaacga ccacgccaga attcagatcg gtgtgctcat tcaggagatc
    12061 gatttgccag tcaacaaagg cgcgcaggaa gttaccttca ccaaactgaa tgatacgttc
    12121 tggatactgt gcaccgggaa aatcgcgacg atttagtgtt ttcacaatgg gttcccttct
    12181 gattagtcat acaacctgtt tgaattggtg cgacaggtta gcaaacttta atacgccgaa
    12241 cccctgtttt gatcaactcc tgatgattaa tgagcagttt tatgagaaaa gtgtggcgcg
    12301 gatcatggtt taatcgagga aatacgcctt ttcctggatc ataaagtggt agaacacatt
    12361 gcattcaaat cgcgcggaat gaataaagat gtcagacaac ttcctcaccg taacgcatag
    12421 tgctggtacg gttgcgccca tctttcttcg agcgatacag acacgtatcg gcttcgaccc
    12481 ttcaatttat taaaatcatc ggtcagcgtt cggtacgatg cgcgaccact accgacgcca
    12541 atacttaccg tgagataaag cgttttttgt tgccaggtga atggttgcag ttcaacgcct
    12601 ttacggattt ttttccgcca ttagcagacc atcgacagga ttcaccgacg gcactgcaac
    12661 agcaaattct tcgccgccca tgcgcgccac cagcccctta tcaccgacaa tcttctgaat
    12721 atgccgggca aacacgctta acactttatc gccacattca tgtccatagt tatcgttaat
    12781 gcttttgaaa tagtcgatat caagcagcat gaccgtcaga tgttgtgtct gtttcagcga
    12841 tggacttttc agcgcctcat aaagaccgga ccgtgagtaa acctgagtca gaaaatcaaa
    12901 gtcggctcgc agcgcaactt gcttcattag cgaattgatc gctgccacgc taaaagaaac
    12961 cataattggg catatcgcca tcgtggcaat accgagacgt gcggagaaca tttccggaat
    13021 ggagaacggc gaaccgaccg agatatcaat caccgaattt gccaccagta cgatttccac
    13081 cgcaccggtg acaaaggtca acagacatgt tacctgcggc gtatagcgca ctgcacacca
    13141 gattaatgca ggcagcggga acgccagact ccccgctccg ccaatgacta ccgaggcaat
    13201 aaccgacaca ataagcgcga tggcgggcat catctgttct gctttaaagc gcggcagcac
    13261 tccaggaata gccaacgtca gcatacaagg cacgatcaaa acgcccgttg agaattgctc
    13321 actgaaccag tccgcaagca aaggccagaa atccagacta tcaatactga ccgaaccaat
    13381 cgccccgaca atagcgcata ataatgcggc aatgagacag taattaaata gccgtaaggc
    13441 acttactggc tcatacttat ttttccccag acgcttatcc cgcacgacca gtaaggcgac
    13501 ggtaacaata aacatcatat tggagaaatt gataaccagt gaaaccagtc cccattcggt
    13561 ggttatggca tcataaacca gcatcgccac ataactgatc gcatagtaat gcagtcgatt
    13621 aagccagaca tagcgggcaa acacccccgc cattacgcca ttcagcggcc agaaaagaga
    13681 aagttcgtga accagccgta actctgcacc aataaaatag aacagcgtgg ttagcgcaaa
    13741 aatagcgatg ctattacgta gcagatgacc ttcctggaac aaacggaaag ttgaaatggg
    13801 gtgtacatgc attaattatc tgtccataca attgcgggtg gcaattatct caacgtaata
    13861 agtaaattct cataaaggat agcatgttcg ctaacagcgg cggcaaataa aggtaccctg
    13921 ggaatgctat tttatcgttt tccgcaattc tcatggctga ttcatctgta caaccagcca
    13981 gtgatagatt accgccacga tataacgttg ttgttcttcg cttaacgaaa taccctgcgg
    14041 aatgttatgc cacttagcca gacaaccgcg acagcaggtc gcggtggcgt gctgagcgat
    14101 aaacaccgga tgcccgcgca tgggggtttg cttaccgtca ttagccggta atgccggagc
    14161 aagacgttta gcgacaaaat ccgccgcatg ttgttcgatg actggcgcac ctttctccag
    14221 gcaatactga cgctctttca tgcccagacg aaagcgagag cgaaattttg aacgggataa
    14281 acgcgcgaag agtggatcga gagactgcat taataaaccg aacgccctaa ctgtttcgtc
    14341 aattgttcaa ggacggcaat acccgcaagc gagttacctg catcatccag ttccggactc
    14401 cagacagcga tggccatttc atgcggaaca atcgccacaa taccgccacc aacgccagat
    14461 ttcgccggta gtcccacccg ccaggcaaac tcccccgcgt tctggtacat accactggtc
    14521 gccatcagcg cgttaatttg ccgcgcctgc attggcgtca ccacaggttc atcaatatga
    14581 atagctttcc cctgattagc cagaaagaca aacgtccggg ccagctctac acagctcatt
    14641 ttcagagcgc agtaatgaaa gtagttttgc agaacggttg tcacatcatg atggaaattg
    14701 ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc aaattcggaa
    14761 cgcgctacca ccgtatcgta tgaaatatca gacacaccgc ttaagccgcg cacgacttcc
    14821 agcatacgtt gccgtggtgc gcttaatcgc ccttgcaaca tatcgcaaac caccagcgca
    14881 ccagcattaa taaacggatt acgcggaata ccctgctcca tttccagttg cactaaagaa
    14941 ttgaacggtg atccagacgg atctttgccg acgcgttgcc agatttcctc ttcggagtaa
    15001 tgacgcatgg cgacaacgag actcagcact ttggaaatag actgaatgga aaaacgttct
    15061 tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac agatagcaat gcccaatcgg
    15121 gaaccgtcta ctgtagccag cgccggaata taatccgcga ctttaccctg accaatgagc
    15181 ggccgcactt gccgcaagat gttttctaaa attgcattat ccatggcgac tgccactttc
    15241 tactcctgga ccgcaggtct gaaaagacct gcgagtatat cagagctgaa tatgtcgcgt
    15301 cagatccggt ctttccacac cgtctggata ttacagaatt cgtgtaagcc gaaatgggaa
    15361 agctcacgac caaagccact ctttttcacg ccaccaaagg ccactcgcgc gtcgctggca
    15421 caataaccat tgataaacac cccaccgcat tccagacgtg ccgccatctg tctggcctgt
    15481 gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact cactatcatt agccagttcc
    15541 agcgcatgtt ctgcatcttt cgcaacggtg attgccgcaa cagggccaaa catttcttcc
    15601 cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg ttggcggata gtagttacct
    15661 gccccagcca tcttttcccc gcccagtaac aaacgcgcac cctgcgccag ggttttctcc
    15721 acctgatgat gcagctcatc acgtaaatca aaacgagcca ttggtccgag agcgttctct
    15781 tcatcacggg gatcgcccat tttcaaggct gccgcagctg ccacaaaacg ttcggtaaat
    15841 gcagaagcaa ttccctcttc gataataaag cgttttgccg ctgcacaaac ctgtccggta
    15901 ttctgataac gtccggctac cgccgcttta accgccagtt ccagatcggc atcgttaagc
    15961 acaataaatg gatccgaacc gcccagttcc agtacgcatt ttttcagtgc cgctccagcc
    16021 tgtgcgccaa tagccgctcc cgcacgaaca cttccggtca ccgtgacagc agcaatgcgc
    16081 gaatctttaa tcatttgact gacaccgtcg ttgtcggcat tcagccagcc atatacgcct
    16141 tgcgggattc ccgcatcttt aaacacctgg gcaatgagct gagcacagcc catcacattc
    16201 ggcgcatgtt taagtaagta gccgttacct gcaagaatga tgggaaccgc gccacgcatc
    16261 acctgccata acggaaagtt ccacggcata atcgccagaa tcgtccccaa cggtcgatac
    16321 tcaataaccg cctgctgatt ttccaccagt gtaggttccg ccttcagcat tgccggacca
    16381 tgttctgcat accagtcaca caaattcgcc gatttcgcca cttcagcgcg cgcctgattg
    16441 attggtttgc ccatttcacg ggtgatcatt tgcgccattt cttcgctacg ggcgcgcaga
    16501 gccttaccga taccacgcag tttttcagca cgataatcta tatttgtctc gcgccagtcg
    16561 cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat cgttagcgcc agcccacggc
    16621 agcacagaaa gttgttcacc cgtggcagga tttattgaaa ttgcatgagt tgccggagta
    16681 atggtcatcg gggtatctcc tttatgagtc atggtatgaa gatacgcaga tttactcttg
    16741 ctttaaaatg aataatatta agccacttat tcacgaatcg agaatgttat ggatctgacc
    16801 caactggaga tgttcaacgc ggttgccgaa gctggcagca ttacccaggc tgcagcaaaa
    16861 gtgcatcgcg taccgtcgaa tctcactacc cgtctacgcc agctggaaac agaactgggg
    16921 gtagatctgt ttattcgcga gaatcagcgt ttacgtctct ctcctgccgg gcataacttt
    16981 ttacgctata gccaacaaat tctcgcgtta gtggatgaag cgcggagcgt tgtcgctggc
    17041 gatgagccgc aaggtttatt ttctcttggt tcgctggaaa gcaccgcagc agtgcgcatt
    17101 ccggccacgc tggcggaatt caaccatcgt tatcccaaaa ttcagttttc actttccacc
    17161 ggcccttccg gcactatgct ggaaggagtg ctggagggaa aactgaatgc ggcatttatt
    17221 gatgggccca ttaaccatac tgccattgac gggataccgg tataccgcga ggagctgatg
    17281 atcgtcacgc cacaaggtca tgcgccagta atccgcgcca gtcaggttaa cggcagtaac
    17341 atttatgcct tccgcgccaa ttgttcgtat cgtcgccact tcgagagctg gtttcatgct
    17401 gacggtgccg ctccgggaac tattcacgag atggagtcgt atcacgggat gttggcctgt
    17461 gtggtcgcag gagcaggcat tgcgcttatt ccacgctcta tgctggaaag tatgccgggg
    17521 catcaccagg ttgaagcgtg gccgttagct gagcaatggc gttggttaac aacctggctg
    17581 gtctggcgtc gcggtgcgaa aacgcgtccg cttgaggcat ttattcaact actggatgtg
    17641 tctgactcgg caaaacagag ttaccaatga gctatttttg atagttcttg cagctcaaat
    17701 gccctatata gtgatgttcc gatgacttat gactatatgg ggcaaatatg gttacgccag
    17761 taagcatcag taattacata tctcttcctg atgattttcc cgtacgtaat attgcacctc
    17821 aggtaaaaga agttttaaag gattttattg atgcactcag taccataata tgtgatgaag
    17881 agtggcgcac gtctttaaac accaactcag ccacaaaaaa gatatttaac aaccttgata
    17941 acttatcata tattcagagg acttcttttc ggggtaacga cacactatac aatgaaaagg
    18001 ttcagtttaa a
//
LOCUS       DAJSKJ010000070        17951 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000070 DAJSKJ010000000
VERSION     DAJSKJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.070"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            222..1853
                     /gene="oppA"
                     /locus_tag="ON345_002087"
     CDS             222..1853
                     /gene="oppA"
                     /locus_tag="ON345_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR3072145.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGAAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1939..2859
                     /gene="oppB"
                     /locus_tag="ON345_002088"
     CDS             1939..2859
                     /gene="oppB"
                     /locus_tag="ON345_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR3072146.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2874..3782
                     /gene="oppC"
                     /locus_tag="ON345_002089"
     CDS             2874..3782
                     /gene="oppC"
                     /locus_tag="ON345_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR3072147.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3794..4807
                     /gene="oppD"
                     /locus_tag="ON345_002090"
     CDS             3794..4807
                     /gene="oppD"
                     /locus_tag="ON345_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR3072148.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4804..5808
                     /gene="oppF"
                     /locus_tag="ON345_002091"
     CDS             4804..5808
                     /gene="oppF"
                     /locus_tag="ON345_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR3072149.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMIIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5861..6190)
                     /locus_tag="ON345_002092"
     CDS             complement(5861..6190)
                     /locus_tag="ON345_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR3072150.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6225..7685)
                     /gene="cls"
                     /locus_tag="ON345_002093"
     CDS             complement(6225..7685)
                     /gene="cls"
                     /locus_tag="ON345_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3072151.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7828..8001
                     /locus_tag="ON345_002094"
     CDS             7828..8001
                     /locus_tag="ON345_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR3072152.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8056..9309)
                     /gene="kch"
                     /locus_tag="ON345_002095"
     CDS             complement(8056..9309)
                     /gene="kch"
                     /locus_tag="ON345_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR3072153.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            9813..11006
                     /locus_tag="ON345_002096"
     CDS             9813..11006
                     /locus_tag="ON345_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3072154.1"
                     /translation="MITDTKLRKALGKKRDDIEIISDSHGLNARISQAGKISFFYRYR
                     WAGKAVKLNVGDYPAMSITQARERRQQFRNWLTEGLDPREQVKLDKQTRQEAMSVAEA
                     FNYWIERHCIANGLVKVDYYRQVFEKHIAEPMKNVKVDNTAKMHWINVFDSIESRVMA
                     HYMLSLCKRAFRFCVNRSVIASNPLEGLLPSDVGQKPKKRTRRMDDDDLRKIYQWLKS
                     HMSIESVFLVKFIMLTGCRTAEIRLSERSWFRLDDNEWVVPAGSYKTRVHIRRGLSDA
                     AVNLVRNHLKKINTNHLVTSQRKIDGGIKDSPVHSPVASNYARSIWNGTGMAEWSLHD
                     MRRTIATNLSELGCPPHVIEKLLGHQMVGVMAHYNLHDYIDDQKHWLRVWQSHLEEII
                     GEPFS"
     gene            complement(11242..11484)
                     /locus_tag="ON345_002097"
     CDS             complement(11242..11484)
                     /locus_tag="ON345_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4060 family protein"
                     /protein_id="HCR3072155.1"
                     /translation="MRLINRGSKQSPLARQACDIALAAHLQTYGDYGRSKMKETYTVK
                     VEGVKVWVEVVNRKASYVATAMTGMRRLRSLPGQVG"
     gene            complement(11523..12635)
                     /locus_tag="ON345_002098"
     CDS             complement(11523..12635)
                     /locus_tag="ON345_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCR3072156.1"
                     /translation="MENTNIVTAEQQTPNTISASNAIFNVQALTQLQSVAGLMAQAAV
                     TVPEHLRGNPADCMAIIMQAMQWGMNPYAVAQKTHLVNGVLGYEAQLVNAVISSSNAI
                     VGRFHYEYEGDWSKCASSREITVKKPAKGGGTYDKKEMVRGWESADEQGLSVRVGAVI
                     RGESDITWGEPVFLSSVITRNSPLWVSNPKQQIAYLALKYWARLYCPAVVLGVYTPDE
                     IEQRTEKEINPTPQRVSLADISGDTVTTTQSAQESSVNVDSLADDFREHIESAQDVDS
                     AKSLRADIETAKATLGSALFTELKNKAVKRYYLVDARNKVEEAIKSLPQPDEPHAAER
                     FAEAERMLASSKRHLGDELHDQFSITLADMKPEYVA"
     gene            complement(12647..16036)
                     /locus_tag="ON345_002099"
     CDS             complement(12647..16036)
                     /locus_tag="ON345_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HCR3072157.1"
                     /translation="MEFFNVVKATQKSGKQDAVVWFTAKTEARANLMLDVALEDAGIE
                     TGRGKDYAKPIRTDFPVVDGLPKEGEVDFTWCDRYELQDDGRTWLPKAAGVSTGSVDA
                     PYTPTPTVIVEDATASEIVPVENRTPAVRFAVHLMNDKYQTHVTKEQQLAASEMSLDE
                     GNTYLHSLLVARNDVPATAKLSLNAEWKMIRAVKDIFTPDEEHEPRLIAAFMSDWVNT
                     DAGDRNQLVEDWRSGKLQLLKTETSNAADVTTGQDLTVEDGIQTDENGRAEGGVVDGE
                     VDTEEQSQQTQQPNLIVVATLPFRQRVLAQFIGDGEYLYHIDAGQKNEIVRLEMDTDD
                     AYVQNLLLAAENVEAFKKAIEHDIHKVVNAVKKVFPVDGKIPELATLIQFLKLWFATD
                     HIDRGILVREWAAGNRISSVQRTDSGTNADGGYVTDRGPDAHHTLDTLDLEIACALPP
                     MDFNHFEIPGSILRRAKEIVTKKEEPWKSWSNILRNQPGVLGVNRTAIFNLVRIAPEN
                     IHLTPVAHLEFVNQTMTAAFNSAVELLPLHETEPAAQEIPQPEGKESPRKSFCTHEEN
                     LQRVREEGARRRAEEAAAQPQKVEQELVKNVGNGIFDVTALLQNSATHGTKKATETTS
                     NVQVQETVSDEKQAGDEVQPGESSLESGEESDTSQKDDVDQNTDSVAKNSDSVSQTEP
                     VAAQTEPEAQSDEPAVVYPAYFEPGRYEGLPNEVYHAANGISSTQVKDARVSLMYFNA
                     RHVEKTIVKERSAVLDMGNLVHALALQPELLDAEFSVEPVIPEGAFTTAATLRAFIDE
                     HNASLPALLSADDIKVLLEEYNATLPPQVPLGANLEETAQNYMALPADFQRIDGDQKQ
                     TATAMKACIKEYNATLPPPVKTSGSRDALLEQLAIINPDLVAQEAQKPAPLKVSGTKA
                     DMIQAVKAVKPDAVFADELLDAWRDNPEGKVLVTRQQLSTALNIQKALLAHPTAGMLL
                     THPSRAVEVSYFGFDEETGLEVRVRPDLEIDLDGVRIGADLKTISMWNVKQESLRARL
                     HREIIERDYHLSAAMYCETAALDQFFWIFVNKDENYHWIAIIEASAELLELGMLEYRK
                     AMRNIATGFDTGEWPAPITADYTDELNDFDLRRLEALRTQA"
     gene            complement(16176..16337)
                     /locus_tag="ON345_002100"
     CDS             complement(16176..16337)
                     /locus_tag="ON345_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072158.1"
                     /translation="MLDKKCGYCGKPVKTEEVIKSTLLYRNGSQLARKEKEYCSKRCA
                     SHDQMAHEG"
     gene            complement(16349..16555)
                     /locus_tag="ON345_002101"
     CDS             complement(16349..16555)
                     /locus_tag="ON345_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HCR3072159.1"
                     /translation="MVSHHYGTQTVNRGAVLPGMLVKHRESTWTASANKRGRLYLHRG
                     IERTYTTDLLVEVYLNGLGHGLSH"
     gene            complement(16548..16736)
                     /locus_tag="ON345_002102"
     CDS             complement(16548..16736)
                     /locus_tag="ON345_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072160.1"
                     /translation="MEQIQGEGGSVVGFGRIRKRSVLGCGMSRNGIRSLVIALAIGVV
                     FWAGLAVEIMHFTGVFNG"
     gene            16983..17186
                     /locus_tag="ON345_002103"
     CDS             16983..17186
                     /locus_tag="ON345_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072161.1"
                     /translation="MSNEDEFFAEMHPQIAQVIGIAVMQLLVERREPSREALIEMIQV
                     LWQGDQVDLAVELALDVLMLREE"
     gene            complement(17232..>17951)
                     /locus_tag="ON345_002104"
     CDS             complement(17232..>17951)
                     /locus_tag="ON345_002104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HCR3072162.1"
                     /translation="DVRVIETPTKLKAITNDFGLEALENSQKQLETILTSGLSIEQAS
                     LFFQGSFALSSVGTFHAANEREYELKINELNKLFITPERAKKRTEVSQKRIITELKDR
                     FEKEGVMGKNLKEISEHKVVQGYPLSESEGLYAELLLKNGIYHLTETLDLRISNNRQK
                     MGESAFKAVTINAAKTKWNNCVKTFVVYAADTPQEKAHSQQLNLVGGYADNIFNLLSS
                     EDMSKYYDHMLVAAGKSLNFN"
BASE COUNT         4380 a         4360 c         4509 g         4702 t
ORIGIN      
        1 aagagcgtcg cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa
       61 ggtgatcgtc tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag
      121 taacctgaca gcagaaattc tccgagcctg tgcagggtcc caatccggga ttacacatgc
      181 tggttaatac cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga
      241 gaagtttagt agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggctg
      301 ctgatgtacc cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt
      361 cagaagttca gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc
      421 gagacctgtt tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgccg
      481 ctgaatcctg ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga
      541 aatggtctga tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg
      601 ttgatccgaa cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta
      661 ttgatgaaat tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg
      721 atcacacatt agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc
      781 acccatcaac ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc
      841 agcctggtaa tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac
      901 gaatcgttct tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg
      961 taacctattt gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa
     1021 tcgacatgac ttataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc
     1081 cggacgaagt tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga
     1141 aaccgccatt caacgatgtg cgtgtgcgta ctgcactgaa actaggtatg gaccgcgata
     1201 tcattgttaa taaagtgaaa gcgcagggcg acatgcccgc ctatggttac actccaccgt
     1261 atactgatgg cgcaaaattg actcagccgg aatggtttgg ctggagccag gaaaaacgta
     1321 acgaagaagc taaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca
     1381 tcaacctgtt gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat
     1441 tgtggaagaa aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc
     1501 tcgacacccg tcaccagggt acttttgatg tggcccgtgc aggctggtgt gctgactaca
     1561 acgaaccaac ttccttcctg aacaccatgc tttcgaacag ctcgatgaat accgcgcatt
     1621 ataagagccc ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc
     1681 agcgcacagc tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc
     1741 ctgtttatta ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca
     1801 aagatccgct ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat
     1861 acgtggggca ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac
     1921 aggccagaag gtagggcaat gttaaaattt attctacgtc gctgtctgga agcgattccg
     1981 acgctattta ttcttattac tatttcgttc tttatgatgc gcctcgcgcc gggaagccct
     2041 tttaccggcg aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat
     2101 cttaatgatc cgatcatgac acagtatttc agctacctga aacaactggc gcacggtgat
     2161 ttcggtccat cgtttaaata taaagattat tcggtcaatg acttggttgc atccagtttt
     2221 cccgtttctg ccaaactggg agccgcagca tttttccttg cggtaatact gggtgttagt
     2281 gctggcgtta ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg
     2341 gcaatgaccg gggttgttat ccccagtttt gtggttgcac cattattagt catgatattt
     2401 gcgatcattt tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg
     2461 atattgccga tggtggcgtt gtcactcgct tatatcgcca gtattgcgcg tattacccgt
     2521 ggctctatga ttgaagtatt acactccaat tttattcgta ctgcccgggc gaaagggtta
     2581 cctatgcggc ggatcatttt acgtcacgca ttaaaacctg ctctgttacc cgtgctctct
     2641 tatatgggcc ccgcatttgt cggcattatt accggttcta tggttatcga aaccatttat
     2701 ggtttgccgg ggattggaca actgttcgtt aatggcgcat tgaaccgtga ctattcctta
     2761 gtgttaagcc tgaccatcct ggttggtgct ttaaccattt tgtttaatgc cattgtcgat
     2821 gtgctatatg cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt
     2881 taagtaagaa aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc
     2941 gcagcttgtg gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc
     3001 tgatagtgct ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg
     3061 cctatgacga tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact
     3121 actttggtac tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta
     3181 tctcactcat ggtcggtgtt gctgcggcac tggtggctgt ggtcgtcggg acactttatg
     3241 gttcgctttc cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa
     3301 tcctcaactc cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggtcaaa
     3361 acatcctgct gattttcgtg gcgataggca tggtttcctg gctggatatg gctcgtattg
     3421 tgcgtgggca aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg
     3481 gtgtatcgac atcgggcatt gttattcgcc acattgtgcc gaatgtactc ggtgtggtgg
     3541 tggtctacgc atcacttctg gtgcccagca tgatcctctt tgaatctttc cttagcttcc
     3601 tggggttggg tacgcaagag ccgttaagca gctggggtgc attgctgagt gatggcgcga
     3661 actcgatgga agtctctcca tggttattgt tgttcccagc gggattcctc gtggtgacgc
     3721 tgttttgttt caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt
     3781 aaggagtgca gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc
     3841 tgacgcactg ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt
     3901 cacggcggtc aatgatttga atttttccct acgtgccgga gaaacgctgg gtattgtagg
     3961 tgagtctggt tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa
     4021 cggacgtatt ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg
     4081 tgaactcaat aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc
     4141 gttgaatccc tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa
     4201 catgagcaaa gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc
     4261 ggaagcgcgt aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg
     4321 agtcatgatt gcgatggcct tgctatgtcg acctaagctg ctgattgcgg atgaaccaac
     4381 aactgcgctg gacgtcaccg tacaggcgca gatcatgacg ctattaaatg aactgaagcg
     4441 ggaatttaat accgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg
     4501 cgacaaagtg ctggtaatgt acgccgggcg cacgatggaa tatggcaacg cgcgcgatgt
     4561 cttttatcaa cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtctcga
     4621 tgcggaaggt gaaacaatgt tgaccatccc gggtaatccg ccaaacctgc tgcgattacc
     4681 gaaaggttgc ccgttccagc cacgttgtcc gcatgcgatg gaaatttgta gtagcgcacc
     4741 gccgctggaa gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact
     4801 gttatgaatg ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg
     4861 cactttgaaa tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc
     4921 gtcgatggtg tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg
     4981 ggatgcggta agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt
     5041 catgttgcct ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt
     5101 cgcagtgata ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgatc
     5161 atcggcgaga tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa
     5221 gttcgcgagc gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac
     5281 cgctatccgc atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt
     5341 attcttgaac cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt
     5401 caggcgcagg tggtcaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt
     5461 tttatcgctc atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat
     5521 ctcggccatg cggtagaact ggggacctat gatgaggtct accacaatcc actacatcct
     5581 tacaccaggg cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa
     5641 accatccagt tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt
     5701 ttccgtaccc gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag
     5761 gggagtttca gacacgccgt ttcttgcctg aaagtcgatc cgctttaaac acaataaggg
     5821 ctgacagttg tcagcccttt ttcacgccga aagcgattat ttattcccgc cagatgatat
     5881 ggcaaagttt gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgttgattt
     5941 caccatcttc actgtccgcc agaccaatca ccacttcggc aaaaaagtct gggttcaaat
     6001 cgaaatcaac atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct
     6061 cttcaaactg aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt
     6121 cgagaaaaat atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat
     6181 ccatatccat agtcactacc tgtttaacct ctgttggcga cgttttacag caacggactg
     6241 aagaagtaaa atagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg
     6301 gcatcgagca gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca
     6361 aaacctttat cgtcgattgc cagggtaatc tcgaaattca gccacagact ccgcatatca
     6421 aggttaacgg tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt
     6481 aacccgcctt caaactgata aattttaacc ccagcagcaa gaagttccgt aaagaatgcg
     6541 cgactggccc agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc
     6601 accccgcgct gtgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag
     6661 ggcgtggtca tgatcaaata ttcacgcgcc gaataagccg cagtcaataa tgcctggtga
     6721 atgagatctt ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc
     6781 tgttcaaacg gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca
     6841 atctcccagt cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc
     6901 gccatcagat caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc
     6961 atattcatgc tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg
     7021 cgcaggtcca tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact
     7081 tcaataccgg cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg
     7141 gcggagtcga gcatcaatcg gcaatgaatg ccgcgccgtg cagccgccat taatgattca
     7201 gccacctggt ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg
     7261 cgcgcgagct ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagt
     7321 tgtagctgat tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat
     7381 ggcgcagcga cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta
     7441 agccattttg cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa
     7501 tggagctcgc caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc
     7561 aacagccagg ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg
     7621 cctgcaatga gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt
     7681 gtcatagatt agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat
     7741 ctggcaaata aaaacgcggg aaaagcactg gtcagcgtcg ggggtggggg tataatgacc
     7801 attctgttat tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg
     7861 cgcatgcagc gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga
     7921 aatatgcgta tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg
     7981 tttgcgatct acaatatcta aatatactgg gcaccattgc ggtgcccaat aagtttactc
     8041 tttcgtcttt aaactctatt tttgcactga ttctttactg tcagctttcg tttcttgttc
     8101 atcgttatcg ctgaaaatgc catgaccgga gttcaacaac attgaaacga gcatatcatt
     8161 attaatctct tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga
     8221 gagaatgata tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc
     8281 gagaactgtt ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc
     8341 tgcatcgtta tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa
     8401 tactgaactg tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc
     8461 aagttgcttg atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg
     8521 attcagttgc aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc
     8581 tttacgatgc attgtatgat tgtttccttt tacaagtttg ttgaatcccc cgcggataag
     8641 cggaccaaaa attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga
     8701 aatagtgaac aatcgtgctg attcagaaac agggacaata tcgccatagc cgacggtaga
     8761 catggtttct atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc
     8821 ttcgcttaaa taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa
     8881 tgcaaaaatt gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa
     8941 aaacaccagc gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag
     9001 ggtgtaaatt agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg
     9061 gaacagtaag ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt
     9121 tgccagcaag gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt
     9181 gaccgacata ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc
     9241 gagaatgtcg tgccgaaggg tcacccataa atttgttgct gtttgtttga atgtagccca
     9301 gtgactcact gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt
     9361 ctgaacatac atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta
     9421 atcaaaggat ttgagcgagc aactgtacct caggaaaggt cattttcctg aatatgctca
     9481 catcatataa agaaatacag ataaagttat tatctgcttg tggtagtgaa tgcactgacc
     9541 ggctataagg aaaggccaaa caagaacacg gttgcaaaaa ccgtgccctt aaatattgaa
     9601 tctctattca gaacactttc ttaaattgtc atttggcata ttacgaacaa ttccgcgtaa
     9661 aaacgttctg ttacgctaaa cccttatcca gcaggctttc aaggatgtaa accataacac
     9721 tctgcgaact agtgttacat tgcgtgtagc tttgagtggg caactttgtg tacacttttg
     9781 tgtacccaaa aacaaaaatg tgtacccatt caatgatcac cgacacaaag ctcaggaagg
     9841 cgctcggcaa gaaaagagat gatatcgaga ttatttctga ttcgcacggg ctcaacgcca
     9901 gaatcagcca ggccggaaaa atatcatttt tctatcggta tcgctgggcc ggtaaagcgg
     9961 taaaactcaa tgttggtgat tatcctgcaa tgagtatcac ccaggcaaga gagcgtcgcc
    10021 aacaattcag aaactggtta actgagggac tggatccgcg agagcaggtg aagctggata
    10081 agcagacccg acaggaagcg atgtccgttg ccgaagcgtt caattactgg attgaaaggc
    10141 actgtatcgc taacgggcta gttaaagtcg attactatcg ccaggtgttt gagaaacata
    10201 tcgccgaacc gatgaagaat gtcaaagtcg ataacacagc gaaaatgcac tggatcaacg
    10261 tcttcgattc tatagaaagc agggtgatgg ctcattacat gctttcgctg tgcaaacggg
    10321 cgtttaggtt ctgcgttaac agaagtgtga tcgcctcaaa cccactcgag ggattactgc
    10381 catctgatgt cgggcaaaag cctaaaaaga gaactcgcag gatggacgat gacgatctgc
    10441 gcaaaatcta tcagtggttg aaaagccata tgtcgataga gtccgttttc ctggtgaaat
    10501 ttattatgct taccggatgc cgtacggctg agattcgact tagtgagaga tcatggtttc
    10561 gattggatga taatgagtgg gtcgtgcctg cgggcagtta taaaactcgg gtacatatta
    10621 gaaggggact ctcagacgcc gccgttaacc tggtcagaaa tcacctcaag aaaataaaca
    10681 ccaatcacct ggtgacttca caacgtaaaa ttgatggcgg gatcaaagat tcgcccgttc
    10741 attcacctgt ggcatccaat tacgcccgtt ctatttggaa tggaacaggt atggcagagt
    10801 ggtcgcttca tgatatgagg cggacgatag ccacaaatct ctctgagtta ggttgcccgc
    10861 cgcacgtaat tgaaaagctg ctcgggcatc agatggtggg ggttatggcg cattacaacc
    10921 ttcatgacta tatcgatgat cagaaacact ggctccgcgt ttggcagagc catcttgaag
    10981 agatcatcgg agagcccttc agttaattta tcttcttttt atcctcccac tctttgattg
    11041 actcagagcg ccagcggtta gggttgccgg gccagtcagg gggtgggaac gggcatacga
    11101 agccccgagg cattgtgtct gcactttgcc atgaccaaag ggttttgcgt gaaattttgt
    11161 agcgactggt caggtctgac gttaccaaaa tatcatccat agctctctcc agttgcccgt
    11221 tcgggccatt caaaatcttt ttcaaccaac ctgcccgggc agggagcgga gacggcgcat
    11281 gccggtcatc gctgtggcca cgtagctcgc ctttcggttc accacctcca cccagacttt
    11341 cacgccttca accttcaccg tataagtctc tttcatcttg cttcgcccat agtcgccata
    11401 tgtttgcaag tgagctgcca gcgcgatgtc gcatgcttgg cgagctaaag gtgattgctt
    11461 acttcctcga ttgatcagtc gcatataatc tccttgaggg agggttaccc ctcccggtct
    11521 cgtcaggcca cgtattccgg tttcatatcc gccagggtga tgctgaattg atcgtgcagt
    11581 tcatcgccta agtgacgctt tgaagatgca agcatgcgct cggcttcagc gaaccgttcg
    11641 gctgcatgcg gctcgtcggg ctggggcagg gatttaatag cctcctcaac cttgttgcgt
    11701 gcatccacta ggtaataacg ctttacggct ttgtttttca gctcggtgaa tagtgcggat
    11761 cccagcgtag ctttcgccgt ttcaatgtcg gcacgcagcg atttggcgct atccacgtcc
    11821 tgagcagatt cgatgtgttc gcggaaatca tcggcaagag agtcgacatt taccgacgat
    11881 tcctgtgcgc tttgcgtggt tgtgacggtg tcacctgaga tatcagccag gctaacgcgt
    11941 tgcggcgttg ggttgatctc tttttctgtg cgctgttcaa tctcatcagg ggtgtacaca
    12001 ccaagaacaa ctgcagggca atataggcgc gcccagtatt tgagtgccag ataagcgatc
    12061 tgctgtttcg ggtttgatac ccaaagtgga gaattacgtg tgattacgct ggagaggaaa
    12121 acaggctctc cccaggtgat atcactttca ccgcgaataa cggcacctac ccgtaccgac
    12181 agtccttgtt catcagcact ttcccaaccg cgtaccattt ctttcttgtc gtacgtcccg
    12241 ccacctttcg caggcttttt aacggttatc tcgcggctgc tggcacattt cgaccagtcg
    12301 ccctcgtact catagtgaaa gcggccaacg atggcgtttg agctggagat caccgcatta
    12361 accagttgcg cttcgtatcc caggacaccg ttaaccaggt gcgtcttttg cgccacggcg
    12421 taagggttca taccccactg catcgcctgc atgatgatgg ccatgcagtc tgccggattg
    12481 ccgcggaggt gctcaggcac cgttacggct gcctgtgcca tcaacccggc gacagactga
    12541 agctgggtta aagcctgcac gttgaaaatg gcattgctgg ctgagatcgt gtttggagtc
    12601 tgctgttcag cggttacgat attcgtgttt tccatcatca ttccccttat gcctgagtac
    12661 gcagcgcttc aaggcggcgc aggtcgaagt cgttcagttc gtcggtgtag tcagcagtga
    12721 ttggcgctgg ccattcacct gtgtcgaatc cggttgcgat attgcgcatc gctttgcggt
    12781 actcgagcat acccagctcc agtagttcag cggatgcctc gatgatggcg atccagtggt
    12841 agttctcgtc tttgttgacg aaaatccaga agaactgatc cagcgctgcg gtttcgcagt
    12901 acatagccgc gctcaggtga taatcacgtt caataatttc ccggtgtagc ctggcgcgca
    12961 ggctttcctg cttaacattc cacatgctga tggttttcag gtctgcacct atacgcacgc
    13021 catccaggtc gatctcaagg tcagggcgca cacgaacttc caagcccgtc tcctcgtcaa
    13081 aaccaaagta gctcacctca acggctcggc tagggtgggt cagcagcatg ccggcggtcg
    13141 ggtgtgccag aagcgctttt tgaatattca gcgcggtgct cagctgctgg cgggtgacca
    13201 gcacttttcc ttccgggtta tcgcgccagg catccagcag ttcgtcggca aatacggcat
    13261 ctggtttgac tgccttcacg gcctggatca tgtctgcttt ggtaccggac actttcagtg
    13321 gtgccggttt ctgtgcttcc tgagccacaa ggtcagggtt gatgattgcc aactgctcca
    13381 gcagcgcgtc acggctgccg ctggttttaa ccggcggcgg cagggtggcg ttgtactctt
    13441 taatgcatgc cttcattgcc gtcgccgtct gcttctggtc accatcaata cgctggaagt
    13501 cagctggcag cgccatatag ttctgtgccg tttcttccag gttagcgcca agcggaacct
    13561 gcggcggcag ggtggcgttg tactcttcca gtaacacctt gatgtcgtcg gcagacagca
    13621 gcgccggcag gctggcattg tgctcatcga taaaggcgcg cagggtcgcg gccgttgtga
    13681 atgcgccttc agggatcacc ggttcaacgc tgaattctgc gtccagtagt tcaggctgca
    13741 acgccagcgc atgcaccaag ttgcccatgt ccagcaccgc tgagcgctct ttgacgatgg
    13801 ttttctctac gtggcgcgca ttgaagtaca tcagcgaaac gcgcgcatct ttcacctggg
    13861 ttgagctgat gccgttggcg gcgtggtaaa cctcgtttgg cagcccttca tagcggcctg
    13921 gctcgaaata agcgggataa acaacagctg gttcgtcaga ttgcgcttct ggctcggttt
    13981 gtgctgcaac tggttcggtt tggcttacag aatcgctatt tttggcgaca gaatccgtat
    14041 tctggtctac atcgtccttc tggctggtat ctgactcttc accagactcc agactgcttt
    14101 cgcctggctg tacttcatca ccagcttgtt tttcatcact gacagtttct tgaacctgca
    14161 cattgctggt ggtctccgta gcctttttcg tgccatgagt tgctgagttc tgcagcaaag
    14221 ccgtaacgtc gaatattccg ttgccgacat ttttaaccag ttcttgttcg actttctgcg
    14281 gttgtgctgc cgcttcctct gcgcggcggc gtgctccttc ttcacgcacg cgttgcaggt
    14341 tctcttcgtg agtgcagaag gatttgcgcg gagactcctt accttcaggt tggggaattt
    14401 cctgtgctgc gggttcagtc tcatgcaacg gcaataactc gaccgcggaa ttgaacgcgg
    14461 ctgtcatggt ctggttaaca aattccaggt gagcgacagg agttaaatga atattttccg
    14521 gtgcgatacg taccaggtta aaaatagccg tgcggttaac acccagaacg cctggctgat
    14581 tgcgcaggat gttgctccat gatttccatg gttcttcttt tttggtcacg atttctttag
    14641 cgcgacgaag aatgctgccc gggatctcga agtggttgaa gtccataggc ggaagggcac
    14701 acgcaatctc taaatcgaga gtgtccagtg tgtggtgtgc gtcaggtcca cggtcagtga
    14761 cgtaaccgcc gtcggcatta gtgccggaat cagtacgctg cacactactg atgcgattac
    14821 cggcggccca ttcgcgaacg aggataccgc ggtcgatgtg atctgtagcg aaccacaatt
    14881 tcaaaaactg gattaaggtt gcgagttcag ggatttttcc atcgacaggg aagactttct
    14941 taacggcatt cacgacttta tgaatatcgt gctcaatggc ttttttgaat gcttccacat
    15001 tctcagctgc cagcagcagg ttctggacgt acgcgtcatc ggtgtccatc tcaaggcgga
    15061 caatctcatt tttctgccct gcgtcgatgt gatagagata ttcaccatca ccgatgaact
    15121 gagccagtac gcgctggcgg aatggcaggg tggcaacaac gatcaggttc ggttgctgtg
    15181 tctgctggga ttgctcttcg gtatcgactt caccatcaac gacgccacct tctgcccggc
    15241 cgttctcgtc ggtctggata ccgtcctcaa cagtgagatc ttgacccgtt gtaacgtcag
    15301 cagcgttgct ggtttcagtt ttgagcaact gcaacttacc actgcgccag tcttctacca
    15361 gttgattgcg gtcaccggca tcggtgttca cccagtcaga catgaatgca gcgatcaatc
    15421 ttggttcgtg ctcttcgtct ggtgtgaaaa tgtccttaac cgcccgaatc attttccact
    15481 cagcattcag gctgagtttg gcggtcgcgg gcacatcgtt ccttgccaca agcaggctat
    15541 ggagatatgt attgccttca tccagtgaca tttcgctggc agccaactgc tgctctttag
    15601 tgacgtgggt ttggtattta tcgttcatca gatggacggc aaagcggacc gctggagtac
    15661 ggttttcaac cgggacaatt tcggacgcag tcgcatcttc aacgattacg gtcggagtag
    15721 gtgtgtaggg ggcgtcaacg gaaccagtag acacaccagc ggcttttggc agccaggtgc
    15781 gcccatcgtc ctgaagctcg tagcgatcac accaggtaaa atcaacttca ccttctttcg
    15841 gcaggccgtc gacaactggg aaatcagtgc gaatcggttt ggcgtagtcc ttaccccgac
    15901 ctgtttcgat acctgcatct tccagcgcaa catccagcat cagattggcg cgagcctcgg
    15961 ttttagcagt gaaccagacc actgcatctt gctttccgga tttctgagtg gctttaacca
    16021 cattaaagaa ttccatgtga gatcctcatt tttgggtgtt agaatccccg gaccattgat
    16081 agcgcccatt gggttaactt tggttttaat gttgtttccg gtgtaacttt ggtcggtacc
    16141 accggacgta gatcccgcct tgcgcgggtt ttacgttagc cttcgtgagc catctggtcg
    16201 tgcgaagcgc aacgtttgga gcaatactct ttttctttgc gcgccagctg tgagccgttg
    16261 cgatagagaa gggtgctctt gattacttcc tccgttttaa ccggcttgcc gcaatatccg
    16321 catttcttgt ctaacatgac atcctccgct agtggctgag tccatgcccc aacccgttca
    16381 gataaacttc aaccagcaag tcggttgtgt aagtccgctc aatcccacga tgcagataca
    16441 ggcggccgcg tttatttgct gatgctgtcc aggtgctttc ccgatgctta acgagcatcc
    16501 ctgggagaac ggcgccgcgg ttaacagtct gtgtcccgta atgatgacta accattgaac
    16561 acccccgtga aatgcataat ttcgacagcc aggccagccc agaaaaccac accaatggcc
    16621 agcgcgataa ccagtgagcg aatgccgttt ctgctcatac cgcaccccag cactgaacgc
    16681 ttacgaatgc gaccaaagcc aacaacagag ccaccttcac cttgaatctg ttccacgcag
    16741 gaacctcatg ttctcggatc atctcttcac ctttgcctta tcgcggctaa cgggacgttt
    16801 tgacttcacc ccggcgttgc cggtgttgtt tggatgagat gataatagca atgagtatta
    16861 accatagcaa tacgtattgt tattatttga tagctattgc tattaaatca ttgatagcta
    16921 aatgaattta ttttttccta ttgtgctgtt atgctcaaaa aaacgccaaa aagggtagcg
    16981 ccatgtcgaa tgaggatgag tttttcgcag agatgcaccc gcagattgcg caggtcatcg
    17041 ggatagcggt tatgcagctg ctggttgaga ggcgcgagcc atcaagagag gcgctgatag
    17101 agatgattca ggtgttgtgg cagggtgacc aggtagatct ggcagtagag ctggcactgg
    17161 atgtgctgat gctgcgggaa gagtagggca gtaaaaaccc ggcgcggtgg ccgggtgctg
    17221 gcaacagttt gttagttaaa attaaggctt ttcccggcag cgactaacat atgatcgtag
    17281 tatttagaca tatcttcgct acttagcaag ttgaagatat tgtcagcata acctccaacc
    17341 aagttaagtt gctgagagtg cgctttttcc tgaggtgtat cagcagcata aaccacaaaa
    17401 gtcttcacgc agttattcca cttggtctta gctgcgttta ttgtcacagc tttaaacgct
    17461 gactcaccca ttttttggcg attattggaa atccgtagat ctaaggtttc agttaaatga
    17521 taaattccat tttttagtaa aagttcagca taaagacctt cagattcaga aagcggatac
    17581 ccctgaacca ctttatgctc gctaatttcc ttgagattct tacccataac tccttctttt
    17641 tcaaacctat ccttaagctc ggtaataatt cttttttgag aaacttcagt tcttttcttc
    17701 gctcgctcag gtgttatgaa taatttattt aattcattga tttttaattc atattcacgt
    17761 tcattggcag catgaaatgt acctacgctt gacaaggcaa agctaccttg gaaaaagaga
    17821 gaagcctgtt cgatgcttaa gccagaagtt aaaatagtct ccagttgctt ctggctattt
    17881 tctaatgctt caagcccaaa atcatttgta atagctttta gtttagtagg ggtttctatg
    17941 acgcgcacat c
//
LOCUS       DAJSKJ010000071        17900 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000071 DAJSKJ010000000
VERSION     DAJSKJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.071"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(176..316)
                     /locus_tag="ON345_002105"
     CDS             complement(176..316)
                     /locus_tag="ON345_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072163.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            412..1128
                     /gene="arcA"
                     /locus_tag="ON345_002106"
     CDS             412..1128
                     /gene="arcA"
                     /locus_tag="ON345_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR3072164.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1189..2541)
                     /gene="creD"
                     /locus_tag="ON345_002107"
     CDS             complement(1189..2541)
                     /gene="creD"
                     /locus_tag="ON345_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR3072165.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2599..4023)
                     /gene="creC"
                     /locus_tag="ON345_002108"
     CDS             complement(2599..4023)
                     /gene="creC"
                     /locus_tag="ON345_002108"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR3072166.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNSADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4023..4712)
                     /gene="creB"
                     /locus_tag="ON345_002109"
     CDS             complement(4023..4712)
                     /gene="creB"
                     /locus_tag="ON345_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR3072167.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4725..5198)
                     /gene="creA"
                     /locus_tag="ON345_002110"
     CDS             complement(4725..5198)
                     /gene="creA"
                     /locus_tag="ON345_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR3072168.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5409..6278
                     /gene="robA"
                     /locus_tag="ON345_002111"
     CDS             5409..6278
                     /gene="robA"
                     /locus_tag="ON345_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR3072169.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6275..6922)
                     /gene="gpmB"
                     /locus_tag="ON345_002112"
     CDS             complement(6275..6922)
                     /gene="gpmB"
                     /locus_tag="ON345_002112"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR3072170.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6974..7495
                     /gene="yjjX"
                     /locus_tag="ON345_002113"
     CDS             6974..7495
                     /gene="yjjX"
                     /locus_tag="ON345_002113"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR3072171.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7580..7906)
                     /gene="trpR"
                     /locus_tag="ON345_002114"
     CDS             complement(7580..7906)
                     /gene="trpR"
                     /locus_tag="ON345_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR3072172.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7996..9933)
                     /gene="sltY"
                     /locus_tag="ON345_002115"
     CDS             complement(7996..9933)
                     /gene="sltY"
                     /locus_tag="ON345_002115"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR3072173.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPALPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10144..11811
                     /gene="ettA"
                     /locus_tag="ON345_002116"
     CDS             10144..11811
                     /gene="ettA"
                     /locus_tag="ON345_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR3072174.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(12118..13350)
                     /gene="nadR"
                     /locus_tag="ON345_002117"
     CDS             complement(12118..13350)
                     /gene="nadR"
                     /locus_tag="ON345_002117"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR3072175.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13371..14753)
                     /gene="radA"
                     /locus_tag="ON345_002118"
     CDS             complement(13371..14753)
                     /gene="radA"
                     /locus_tag="ON345_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR3072176.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14802..15770)
                     /gene="serB"
                     /locus_tag="ON345_002119"
     CDS             complement(14802..15770)
                     /gene="serB"
                     /locus_tag="ON345_002119"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR3072177.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15876..16520
                     /locus_tag="ON345_002120"
     CDS             15876..16520
                     /locus_tag="ON345_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR3072178.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16548..17564
                     /gene="lplA"
                     /locus_tag="ON345_002121"
     CDS             16548..17564
                     /gene="lplA"
                     /locus_tag="ON345_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR3072179.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17596..17745
                     /locus_tag="ON345_002122"
     CDS             17596..17745
                     /locus_tag="ON345_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072180.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         4280 a         4970 c         4497 g         4153 t
ORIGIN      
        1 ttggattcac cacgtttatt agttgtatga tgcaactagt tggattatta aaataatgtg
       61 acgaaagcta gcatttagat acgatgattt catcaaactg ttaacgtgct acaattgaac
      121 ttgatatatg tcaacgaagc gtagttttat tgggtgtccg gcccctctta gcctgttatg
      181 ttgctgttaa aatggttagg atgacagccg tttttgacac tgtcgggtcc tgagggaaag
      241 tacccacgac caagctaatg atgttgttga cgttgatgga aagtgcatca agaacgcaat
      301 tacgtacttt agtcatgtta cgccgatcat gttaatttgc agcatgcatc aggcaggtca
      361 gggacttttg tacttcctgt ttcgatttag ttggcaattt aggtagcaaa catgcagacc
      421 ccgcacattc ttatcgttga agacgagttg gtaacacgca acacgttgaa aagtattttc
      481 gaagcggaag gctatgatgt ttttgaagcg acagatggcg cggaaatgca tcagatcctc
      541 tctgaatatg acatcaacct ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt
      601 ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt tgatgttcct gactggccgt
      661 gacaacgaag tcgataaaat tctcggcctc gaaatcggtg cagatgacta catcaccaaa
      721 ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc tgctgtcccg taccatgaat
      781 ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct acaagttcaa tggttgggaa
      841 ctggacatca acagccgttc gttgatcggc cctgatggcg agcagtacaa gctgccgcgc
      901 agcgagttcc gcgccatgct tcacttctgt gaaaacccag gcaaaattca gtctcgtgct
      961 gaactgctga agaaaatgac cggccgtgag ctgaaaccac acgaccgtac tgtagacgtg
     1021 acgatccgcc gtattcgtaa acatttcgaa tctacgccgg atacgccgga aatcatcgcc
     1081 accatccacg gtgaaggtta tcgcttctgt ggtgatctgg aagattaatc ggctttacca
     1141 ccgtcaaaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt atttccagat
     1201 acgtaattca tcgtccgttg taacttcttt actggctttc attttcggca gtgaaaacgc
     1261 ataccagtcg atattacgag tcacaaacat catgccggcc agcgccacca ccagcacact
     1321 ggttcccaac aacagcgcgc tatcggcaga gttgagcagt ccccacatca caccatccag
     1381 caacaacagc gcgagggtaa acaacatgct gttgcgccag cctttcaata ccgcctgcaa
     1441 ataaataccg ttcattaacg ccccaatcag actggcgatt atccatgcca cggtaaaacc
     1501 ggtatgttca gaaagcgcca gcaagagcaa ataaaacatc accaatgaaa gccccaccag
     1561 caaatattgc attgggtgta agcgttgcgc ggtgagcgtt tcaaaaacaa agaacgccat
     1621 aaaagtcagc gcaatcagca gaatggcata cttagtcgcc cggtcagtta attggtattg
     1681 atcggctggc gtcgttactg cgacgctaaa cgccgggaag ttttcccagc cggtatcatt
     1741 gcctgaagca aaacgctcac cgagattatt agcaaaccag ctgctttgcc attgcgcctg
     1801 aaaacctgac tcgctaactt cccgtttggc tggtagaaaa tcacctaaaa aactgggatg
     1861 cggccagttg ctggttaagg tcatttcgct attacgcccg gcaggcacca cagaaagatc
     1921 gccggtaccg cttaaattca gtgccatatt cagctgcagg ttctgcttcc gccagtcccc
     1981 ttcgggtaaa gggatatgca cgccctgccc gccttgctct aacccggtac cgggttcaat
     2041 ggtcagtgcc gttccgttaa cttcaggcgc tttcaccaca ccaataccac gcgcatcccc
     2101 gacgctaatc acaataaagg gcttgcctaa gatgatattt ggcgcgttga gctcgctaag
     2161 acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg tgccagacct gaccggtata
     2221 aatccctatc ttgcgttctt ccacgttctg attgccatca accatcaatg actcaggtaa
     2281 ccaaaaatgg ataaaacttc gtttccgctc cacggtttta tcctcttcct gcaccgtata
     2341 aagctcggtc acaggaatag cgataagcgg cccaacgagt ttttgcggcc cgctggtact
     2401 ttggcgaatc gcatcttcca catcgctacg gtaatcggca cgttcgacaa tcacttgccg
     2461 aatcagcatt atcggaatca acaacagcaa tactgcacca aacaaggtgg tcattttcca
     2521 gaacaggggg gatttcaaca tagtcttctc ctttgcaatg gcagcaggat acgaaggcta
     2581 tgtgggaaga atttgaagct atgtgaagtg acggtgaagt cgaagcgagg ccagcacgcc
     2641 accttcctgc acgttgcgca gcgtgacttc gccgttaaac aaacgggcga cctcactgac
     2701 gaacgccaac cccagaccgc tgcttttttg cccatttgca cgcggcaaag agtaaaagcg
     2761 ttcaaaaata cgtgacagcg cgtagtcagg aataccacta ccggtatcca gcaccttaag
     2821 ggcgacgtgt tcctgatcca cttcggcgct tagcgttatg cgaccgctct cgggggtaaa
     2881 atcgatggcg ttatccagta aattccccag cgcctgctcc agtaacgccg gttcagcagc
     2941 aacattaacc tcagtgggca taacatgtag agtgatgttt ttttctgcca actgcacggt
     3001 gcgcgcttcg ctgacgcggc gaaataatgc cgccacatca acaacagtca gaacgacttc
     3061 ctgacgattc tccagtcttg cctggcgtag taacgtttct accaatgcct gcatacgcgc
     3121 attttgcgtc agaatgttgt cggtaaaacg agccaccact tccggtggcg gaccttcgcg
     3181 taaaatttcc gccgcgccac gaatcgccgc cagtgggctt tttagctcat gggttaacgc
     3241 ataaacatac tgctcaatat agtttttccc ttccagcttc acgcgcatac tttccagcgc
     3301 ctgcgcaagc ttacgcaact cgctactgcc gagttcagga agaggaacgg gcttattgtc
     3361 agtgacggaa tcggcatagc gagtgagccg ggcaatggag cggttgatcc accaaaccat
     3421 gcctgcgcca atcaccagtg caatccccag caaaatggcg ctggcccata aaattcgccg
     3481 ctcgctacgc ttaatgaccg gagccatcgc cgcgttcggt ttgcctacgc tcaaaacgcc
     3541 aataagccgc gagccgtcca taatcggcgc ggcaacatac atcaccgaac tttcgggatc
     3601 ggcagaattt tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta gccagacgtc
     3661 attccagcgc gaataatcct gcccaacggc tttatttgcc gaatcgaaca atactttacc
     3721 ctgcgaatcg gtcatataga catgatattc attgcgcact ttgttaatgc caccgatatt
     3781 ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc gccagttgcc catgcgttgg
     3841 gtccccagag agcaaatcgg gacgcgccag ctccgccagc aacgttgcgg tatcgattaa
     3901 cgtcccctcc gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa tagccagtac
     3961 gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc agcaaccgca tgccgatacg
     4021 cattacaggc ccctcaggct atatcccatg ccgcgatgag tattaatcgg tgaaagatcg
     4081 gggttgatgg cgcgcagctt ggcacgcaac gttttaatgt gggtatcaac ggtgcgatcg
     4141 taggtgtcct gcgcatcttc ccacacgcta tccatcagtt gctggcggga ccagacgcgg
     4201 cctggcgact tgagtaacgt cttcagcaat aaaaactcat accgcgtcag cgttaatggc
     4261 gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt caaaatgacc aatacggatg
     4321 acgggagacg gcgacgagaa cttcttcacc cgacgcagta aggtgcgcac cctggcgcac
     4381 acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag cgccaatttc cagcccaagc
     4441 aggcgatcga cctcttcact tcgggccgtc aggaacagta caggtaacgc cggatggagc
     4501 gccagtaact ggcggcataa ttcaaagccg ctgatatcag gcaaaccaac gtcgagaatc
     4561 atgacgtcgg gaaccagctg gcgagcttta tccagcaccg gcaagcctcg ctcaaagacc
     4621 tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg tgtcggctat cccttgctca
     4681 tcttccacta accagaccgt ttcccgttgc ataaatcgcc tctgttattg ccgccacggc
     4741 atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt ctacaacttt gtcggagtaa
     4801 gccagataag cgagcgcgtt gcgtttagca tcataaaagc gcacgacctg taacgactta
     4861 aagaccaggg acgtacgttt tttgaatact acctcgccct gagctttgcc gtttttaata
     4921 cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa tggccgcatc ggaggtatct
     4981 tccgccaggc ccaatccccc tttaataccc ccggttttcg cccggctcac ataacaggtg
     5041 acatttttca catcgggatc gtcaaaggct tccacaacaa ttttgtgatc cgggccgatc
     5101 attttaaata cggtgtcgac cgaaccaatc tcttctgcat gagccaatgg ccccagcatt
     5161 atcaggctta aagacaggat caaatgcttg tatttcatat tgttaccatt tttatctatt
     5221 acacgagcaa ttagttcgtc acggtaaata acatattgat taagaacaaa atctcaatac
     5281 ttttatttcc gtcaagccct aaaacatact ctactaagaa aaaaacactg aatgctaaaa
     5341 cagcaaaaaa tgctattatc caattacctg atgtcaggtg ctcgttgttg aaaggatgag
     5401 gatattttat ggatcaggcc ggcattattc gcgacctttt aatctggctg gaaggtcatc
     5461 tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc aggttattcc aagtggcact
     5521 tacagagaat gtttaaagat gtcactggcc atgctattgg cgcgtatatt cgtgctcgtc
     5581 gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg tccgattctg gacatcgcgc
     5641 tgcaataccg cttcgactct caacagacat ttacccgcgc attcaagaag cagtttgccc
     5701 agactcctgc actttaccgc cgttctcctg aatggagcgc ctttggtatt cgcccgccgc
     5761 tacgtctggg tgaattcaca atgccagagc acaaatttgt caccctggaa gatacgccgc
     5821 tgattggtgt tacccagagc tactcctgtt cgctggagca aatctctgat ttccgccatg
     5881 aaatgcgtta tcagttctgg cacgattttc tcggcaacgc gccgaccatt ccgccagtgc
     5941 tctacggcct gaatgaaacg cgtccgagtc aggataaaga cgacgaacaa gaggtattct
     6001 ataccaccgc gttagcccag gatcaggcag atggctatgt actgacgggg catccggtga
     6061 tgctgcaggg cggcgaatat gtgatgttta cctatgaagg tctgggaacc ggcgtgcagg
     6121 agtttatcct gacggtatac ggaacgtgca tgccaatgct caacctgacg cgccgtaaag
     6181 gtcaggatat tgagcgatac tacccggcag aagacgccaa agcgggagat cgcccaatta
     6241 atctgcgctg tgaactgctg attcctatcc gccgttaacg ctgcagctca tctaatgcag
     6301 gggcgtccag atgcgagatg tcccctgcgg tttcaacgac ccagccagac gccagccaca
     6361 ggctttcctg ataatccacg cgcgaaatag aacagttacg cagacgtaag cggcgctctg
     6421 cccatgctgg taatccgaga atcgtactca ccaggcatcc cagtgcaata ccatgactca
     6481 ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga ttccagtgcc gcattgacgc
     6541 gatcgctgag ctcctgcatt gactcgcctt caggaatacg cccgtcaacg gtgccattga
     6601 ccagctgccg acgccagttc tcttcttctt cggtcagaga atcgatatgt cttttttcca
     6661 gcacacccat gtttaattca cgcaggcgag aatcaaagat gatgtcacag ccgcaggcct
     6721 gggcgatgat ttccgccgta cgccgggtgc gtcctaaatc gctactgata atatgcgtaa
     6781 tgccaagctc tttggcacgg gttgccacct gcatcgcctg ttgctcacct ttggcggtca
     6841 gcgggctgtc agactggccc tgaatacgtc gctcggcgtt ccactgcgtt tcaccgtggc
     6901 ggactaggta tacctgtaac atgctttttt tccgtatact gctgtaacgt taattttctg
     6961 agtaatgctg attatgcacc aagttgtctg tgcgaccacc aatcccgcta aaattcaggc
     7021 cattctgcag gcatttcacg agatcttcgg cgaaggatcc tgccatattg catccgttgc
     7081 cgtcgagagc ggtgtaccgg aacagccctt tggcagtgag gaaacgcgcg ctggcgcacg
     7141 aaatcgggta gcaaatgccc gccgtttact tccagaggct gatttttggg tggcgattga
     7201 agctggcatc gatggtgaca gcactttcag ttgggtggtg attgaaaaca ccacccagcg
     7261 cggcgaggcg cgttcggcta ctttgccgtt acctgcggtg attctgcaga aagtgcgtga
     7321 aggtgaggcg cttggtcccg tgatgtcgcg ttataccggt attgatgaga ttggtcgtaa
     7381 ggaaggggcg atcggcgtgt ttaccgccgg gaaactcact cgcgccagcg tctatcacca
     7441 ggcggtgatc ctggcgctga gtccgtttca caatgccgtg tatcaggcat tgtaggacgg
     7501 ataaggcgtt cacgccgcat ccggcattcg gtgcacgatg cctgatgcga cgctgccgcg
     7561 tcttatcagg cctacaaaat caatcgcttt tcagcaacac ctcttccagc cactggcgca
     7621 gctcaacggg cgcggctttc aggctgttag atccacgcgt aatcgtcgcg atgcccgcgc
     7681 cgagttcatt ttttaactca cgctggctca tttcgccgcg caacagctct tcgacaatac
     7741 gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag catcaggttt aacaacggta
     7801 aatggagatc gttttggtag gcattcttaa gcaggtcgac aaaacgtaac cactcctggt
     7861 gacgctgttc tgccatcgct gctgaatagg gtgattgttg ggccataata tgtcgccatt
     7921 gttagcgggg gaagcaaaat gcctcccccg gttgtactcg ctaaagagta cgatagcata
     7981 tcataaacgt gcggatcagt aacgacgtcc ccattccgtg gcgctcatca acgtcggttt
     8041 atcccccatg aaatagcggt agtaagcgtc ataagccagc acgttcttca cataaccgcg
     8101 cgtctcggag aacggaatac tctcgacaaa tgccacagcg tcgatacgcc cggcgctgtt
     8161 gccaagccag gttcgcaccc gtccaggtcc ggcgttataa gctgctgagg agaaaatacg
     8221 gttattgcca aactgctgat aaacatattg caggtaactg gtgccaatgt tgatattcgt
     8281 ttccggatcc agcaattgcc cagggctgct ataaccagga atagagaaca tcttcaccgt
     8341 atgggtcgct gtaccaggca taatctgcat caggccgctg gcccctaccg gtgatttcac
     8401 tttcggattc caggcgctct cctgacgggc aatcgccatc gcatagcttt gcgggatctc
     8461 tttcccactg gtgtaacgtt tgaaaagatc gttgtaagcc agcgggaatc gctcttccag
     8521 atgatcccaa agcttcccgg cgatcgttgc ctgaacgcta agatcccacc attggttatt
     8581 gaaagcatac cgagccagtt gagcctgctc tgtttttgac ttgctcttca ccagattggc
     8641 ccactcgcta cgcgcggtgt tatcgagatt ccagtacatc aactcgcgca cgcgcgccat
     8701 ctccggcccc tgagtcaggg cgctgtcaac attctgcggc gctttatcaa tcttcagctc
     8761 atactcttct ccgatgcgtt gtgctgcaac catcgggtag aaaccacgct gttgcatgag
     8821 ttgatgcaaa atctctttcg cttcagcttc acgtccgcgt tccagcaata aatccgcctg
     8881 ccagtaacgc cattcatctt tctcttttgc ttccatcggc agacgcgcca gccaggtatt
     8941 caggccgcga cgatcgccgg tgccaagtgc cattcgcaca cggcgttcaa tcaacgacgt
     9001 ggattgcgag cgcataatgg cgtcatcgcg ccatttcgcc tgctcgtcgg tgacatcgtt
     9061 gcccatcaaa cgccaggcga cgatatcgcg cagctcctga atctgatctt cattaagctg
     9121 ctgcgcctgg gcaagcgacg ggatcatcag ccgtgcattt tcagcatcct gccgcgccac
     9181 actggcaaat gccaccgccg ccatttgacg ggtaaaatcg gtcgcgccgg ttgtacgcgc
     9241 gaaggtcagt accgtattag ggttgttcgc cagtgaaatg attgccgagg cgatagtctg
     9301 gtaatcggca ggcatctgcc ctgccagcac tgttaccagg cctgtgttac ccgctttcat
     9361 cgccagacgg atacgctcta aatacgccag cggatcttgt ttacctgacg cacgccagac
     9421 gctaaataac ttgtcacagg cgttaggctg gctcttgccg gttagccaca gctctttcgc
     9481 cccttgccag gcttcttcac tctgcccggt gttccatttc gcatagtagt aattacattg
     9541 cgcttcggta gttccgggct tttccgggct aaaggctaac aagccacgcc agtcttcacg
     9601 ccgcgccagt tcattgacga aacgagattg cagcgtgcga gcgggaggaa gcgcggggtt
     9661 agcgcgaaca aagttagtga ccgtcaccgc cggttgattc atcagatcat cggtgatctg
     9721 gcggtattcc aggtagggat aaagcggata atccttcagt ccaggcatca tttgttcgac
     9781 cacatccatc tgtcgattat cccaggcctg cttgatttgc gcgtaacggc tacgctgctc
     9841 atccagtgag tcggctcgcg ccacgctgct gaccgtcagc agacagacac cggcagccaa
     9901 cagccgccag gtaacttgtt tggctttttc cacaagcgca tcctctaagt gtaaatacat
     9961 caatgcagcg tcatgccgcg taatagattt atgctaacca gtcattgccg tttacgccac
    10021 gttacggaca cttttttact tttactgcga aggtgatcga ccaactggga ttaggcgggg
    10081 aaaaaggcta cacttcgcct ttgaaaactc atcatcacga taaacataga ggcgaagtcc
    10141 aacgtggctc aattcgttta taccatgcat cgtgtcggca aagttgttcc gccgaaacgt
    10201 catattttga aaaacatctc tctgagtttc ttccctgggg caaaaattgg tgtcctgggt
    10261 ctgaacggcg cgggtaagtc taccctgctg cgcattatgg cgggcattga taaagacatc
    10321 gaaggtgaag cacgtccgca gccagacatc aagattggtt atctgccgca ggaaccgcag
    10381 ctgaacccgg aacacaccgt gcgtgagtcc attgaagaag cggtttctga agtggttaac
    10441 gccctgaaac gcctggatga agtgtatgcg ctgtacgccg atccggatgc cgattttgac
    10501 aagctggccg ctgaacaagg ccgtctggaa gagatcattc aggctcacga cggtcataac
    10561 ctgaacgtac agctggagcg tgcggcggat gcgctgcgtc tgccggactg ggacgcgaaa
    10621 atcgctaacc tctccggtgg tgagcgtcgt cgcgtggcgt tgtgccgcct gctgctggaa
    10681 aaaccagaca tgctgctgct cgacgaaccg accaaccacc tggatgccga atccgtggcc
    10741 tggctggaac gcttcctgca cgacttcgaa ggcaccgttg tggcgattac tcacgaccgt
    10801 tacttcctcg ataacgttgc aggctggatc ctcgaacttg accgcggtga aggtattccg
    10861 tgggaaggca actactcctc ctggctggag cagaaagatc agcgcctggc gcaggaagct
    10921 tcacaagaag cggcgcgtcg taagtcgatc gagaaagagc tggagtgggt acgtcaggga
    10981 actaaaggcc gccagtcgaa aggtaaagca cgtctggcac gctttgaaga gctgaacagc
    11041 accgaatatc agaaacgtaa cgaaaccaac gaactgttta ttccacctgg accgcgtctg
    11101 ggtgataaag tgctggaagt cagcaacctg cgtaaatcct acggtgatcg cctgctgatt
    11161 gatgacctga gcttctcgat cccgaaaggg gcaatcgtcg ggatcatcgg tccgaacggc
    11221 gcgggtaaat cgaccctgtt ccgtatgatc tctggtcagg aacagccgga cagcggcacc
    11281 atcactttgg gtgaaacagt gaaactggca tcggttgatc agttccgtga ctcaatggat
    11341 aacagcaaaa ccgtttggga agaagtttcc ggcgggctgg atattatgaa gatcggcaac
    11401 accgagatgc caagccgcgc ctacgttggc cgctttaact ttaaaggggt tgatcagggt
    11461 aaacgtgttg gcgaactttc cggtggtgag cgcggtcgtc tgcatctggc gaagctgctg
    11521 caggttggcg gcaacatgct gctgctcgac gaaccaacca acgacctgga tatcgaaacc
    11581 ctgcgcgcgc tggaaaacgc cctgctggag ttcccgggct gtgcgatggt tatctcgcac
    11641 gaccgttggt tcctcgaccg tatcgccacg cacatcctgg actaccagga tgaaggtaaa
    11701 gttgagttct tcgaaggtaa ctttactgag tacgaagagt acaagaaacg cacgctgggc
    11761 gcagacgcac tggagccgaa gcgtatcaag tacaagcgta ttgcgaagta atgcgtaaaa
    11821 tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc aagaaatttc aatacgttgt
    11881 gaatctccag gcctgataag acgcggtaag cgtcgcatca ggcattgagc accgaatgcc
    11941 ggatacggcg tgaacgcctt ttccggccta caacagcctt agtatccgca atttcgtagc
    12001 gggtcacccg tgctgattct tttaaacctt gtttaatctc aacagcctct tccagagagg
    12061 ttttcaactc atcaaaaaac ttattttata cctcgccttt gagccgtttc atcgcggtta
    12121 tctctgctcc cccatcatct cccgcaccag ctccacgcag cgcaggaaac ggctgtcata
    12181 atcgtcctct tcaacccgca cgaattcgat attgttctct tccagcatct ccaccagcaa
    12241 gttctggaac tctttgcgat ccaccgagct gccgaggctg cgtaaaccat ccgccaccca
    12301 cggcgtgttg ttctccagca ggatcaccag atcgaaacgg tattcatcaa tcaacgcctg
    12361 tacgaacgga tgctcacgcc cttcgtactt tttgcagaac gcctgggtag tgacaaaatc
    12421 agtgtcaata aacgccactt tattggcata tttcactgca aaatcaatgt attgtgcgtg
    12481 gcccagcgcg attttatcgt aatcggaata ctgcaatgcg atctcatcac cgccgaggtg
    12541 tgaaaagaca taatcgcgac catattccca cgcactggtg gtattgaaga tattggcaag
    12601 tttgtttacc agggtggatt taccgctcga ctcgccacca aggatcgcca cggtacgtac
    12661 aaagaacggc ttcacttccg taggaatata ttcccagtag cggaacgggt tttcgcggat
    12721 ctgcgcaccg ctgatactca taaaggtacg tttcggatcg accagcaccg tatcgatccc
    12781 cagatgttcc atatactgtg gcgcatcggc ttcttccgag gtgtagatca ggtccggctg
    12841 aatccccttt tcagccataa actttttgat gccgttgctc cacacatccc agccgtgcgg
    12901 atacggctcc atgccctctt cgttgaaagc atgaatgcga atatttttct gatatttaaa
    12961 agtttgtaat aaccagcgca gacgatccgg caccgtgggc tgctgcgaca tggcgctgtc
    13021 ttcaaacaac gcgcggtcgc gggtatcgtc aaaacccata atgatatgca gctcgtcaac
    13081 ctggctacag gcgcgctgga taaggtagat atgtccggta tgcagcgggt agaacttacc
    13141 gaatacgaca ccaatggttt tcttctgtcg gggaaattca agccccaaaa aacggtgcaa
    13201 cgcctccagc ttttgcgcgc tggggctttt aattttggca ttcagtaact ggcttaaata
    13261 ccctttggtc ataccgctgg catcagctac ctgctgtagc gtgcagccct gttgcttgat
    13321 ggcagttttc aggtaatcaa atgacgacat atctccctcc gtatctctca ttataagtcg
    13381 tcgaacacgc taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat cccttccggc
    13441 gcttttttcg gtacgttagc cgccggaaca atcgcccggc gaaaaccgtg tttcgccgct
    13501 tctgaaattc gttcctgacc gctgggcacc gggcggatct cccctgccag cccgacttca
    13561 ccaaacacca ccagatcctg tggcagcggt ctgtcacgta ggctggaaac catcgccagc
    13621 agtaacgcta aatcggcact ggtttcggtt accttcacgc cgccgaccac gttcacaaat
    13681 acatcctgat cggccatttg cagaccaccg tgacggtgca agacagccag caggattgcc
    13741 agacggtttt gttccagccc cactgccacg cggcgcggat tcgccatcat cgagtgatcg
    13801 accagcgcct gaatctccac cagcaacgga cgcgttcctt cccacaccac catcacggag
    13861 ctgccggagg tcacttcatc tccgcgactt aagaaaattg ccgaagggtt gctgacttca
    13921 cgcagcccct gttcggtcat cgcgaagacg cccagctcat tcaccgcgcc gaagcggttt
    13981 ttatggctgc gcaaggtgcg aaaacgggag tcggcatcac catccagaag caccgaacag
    14041 tcgatacagt gttccagcac tttagggcca gccagcgagc catctttggt tacgtgccca
    14101 accatgacaa tcgccacacc gcgcgttttg gcgaagcgcg tcagataagc cgccgtttca
    14161 cgcacctgcg ccacgctgcc aggcgatgac tgtacatccg ccatatgcat cacctggatc
    14221 gagtcaatta ccatcagctt cggttgctct tcttcggcaa tcaggcagat ctgctcgatg
    14281 ctggtttccg acaacatatt gagattgtca gtcggcaggc caaggcgatg agcgcgcatt
    14341 gccacctgtt gcagcgactc ttcgccggtg acatacagcg ttttcatctg ctgggccagt
    14401 ttgcacagcg tttgcagcaa cagcgtggat ttccccgcac cagggttacc gccaatcaga
    14461 atggcacttc ctggcaccac gccgccgcct agtacgcggt cgaactcttt aaatccggtg
    14521 gaaaaacgcg gcagctcttc aaggctgata tcggagagtt tctggacttt tgccaccccg
    14581 gcgctaccgg catagccgct gagacgctcg ttacgcgcca ccgttggcga cgcagcaaga
    14641 cgcacctcgg tgatggtgtt ccaggcatga caggcactgc actgcccctg ccagcgcgga
    14701 taatcggccc cgcattcatt acaaacaaag gcgcgttttg gagcttttgc cacggtgtac
    14761 ctcgttaatg ctgtgccgcc cgcaggatgg cgggcgagca attacttctg attcaggctg
    14821 cctgagagga tgcagaatac ccccatcagg tcagcgtgac ggatggtgac ttccgccttt
    14881 tcattcactt ttggcttggc atggtaggca atccccagcc ctgctgcttt gatcatcggc
    14941 aggtcattag ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc atactcctgc
    15001 gcgaggcgag tcaaagtttt cgctttgtac tgcgcgtcta cgatgtcgcc gatcacattg
    15061 ccggtaaatt taccgtccat gatctccagt tcattggcta ccacggcggt caggcgcagc
    15121 ttgtcgcgca gatattcagc aaagaaagta aagccgccgg aggcaatcgc cactttccag
    15181 cccagcgttt ccagcttgag caccagttgc gttaagcctg gcatcagcgg cagattttca
    15241 cgcacctgtt gcagaatatt ggcgtcagcg cctttcagcg tcgccacacg gctgcgcagg
    15301 ctggcggtaa aatcgagttc gccgcgcatc gcccgttcgg ttacttccgc caccatctcg
    15361 cccgttccgg ccagtttggc aatttcatca atacattcaa tctggatggc ggtggaatcc
    15421 atgtccatca ccagcaaacc cggcgtgcgc aggtgcggga ttttccccag cggggcgaca
    15481 tccagttgcg cttcatgggc caggcgcgta gcccgtgcgg tgagtgaacc tgccagacga
    15541 atcacctgat aatcttccac gcaccaggcg gcaacaatca ccatcgccgc acccagtttg
    15601 ctctggtatt gggtcagacg ctgtttatcc agcccacgac catacagcag ccagccgcta
    15661 cgacctgcgt ggtaatccag tggcatcact tcatcaccac ttaatgaaag aggcagacca
    15721 ggccataaag agacatcttc aggcaggtcg caccaggtaa tgttaggcat taaggctcct
    15781 gtaaaatcgt tcgaagcagg gaaaataacg catgaggcta ccttgtatcc attgcttctg
    15841 gcaacattaa gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa ctgaaattcc
    15901 ggctgcatcg ggcagtgatt gtcctgttct gtcttgcctt gttagtggcg ctgatgcagg
    15961 gagcgtcatg gtttagtcaa aaccaccagc gacaacgtaa tccacagctg gaagaactgg
    16021 cccgcaccct ggcgcgtcag gtaacgctga acgttgcacc gctgatgcgt accgactcac
    16081 cagatgaaaa acgcattcag gcgatcctcg atcagttaac ggatgaaagc cgtatcctcg
    16141 acgcgggtgt gtatgacgaa caaggcgatc ttatcgcacg ttctggcgaa agcgtcgaag
    16201 tgcgcgaccg gctggcgctc gacggtaaaa aagcgggcgg ctattttaac cagcagattg
    16261 tcgaaccaat tgccgggaaa aacggaccgc tcggctatct gcgcctgacg ctcgacaccc
    16321 atacgctcgc caccgaagcc caacaggtgg ataacaccac taacatttta cgcctgatgt
    16381 tgctgctttc actggcaatc ggcgtagtgc tgacccgcac gctgctacag ggtaaacgta
    16441 cccgctggca gcaatcgccc ttcctgttaa ccgccagcaa accggtgccg gaagaggaag
    16501 aaagcgagaa aaaagagtga cccattacta caagaaagga aatcgttatg tccacattac
    16561 gcctgctcat ctctgactct tacgacccgt ggtttaacct ggcggtggaa gagtgtattt
    16621 ttcgccaaat gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc gacacggtag
    16681 tgattggtcg cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg gaagaagata
    16741 acgtccgcct ggcgcggcgc agtagcggtg gcggcgcggt gttccacgat ctcggcaata
    16801 cctgctttac ctttatggct ggcaagccgg agtacgataa aaccatctct acgtcgattg
    16861 tgctcaatgc gctgaacgcg ctcggcgtca gcgccgaagc gtccggacgt aacgatctgg
    16921 tggtgaaaac agtcgaaggc gaccgcaaag tctcaggctc ggcctatcgc gaaaccaaag
    16981 atcgtggctt ccaccacggc accttgctgc tcaatgccga ccttagccgc ctggcaaact
    17041 atctcaatcc ggataaaaag aaactggcgg cgaaaggcat tacctcagtg cgttcccgcg
    17101 tgaccaacct caccgagctg ctgccgggga tcacccatga gcaggtttgc gaggccataa
    17161 ccaaggcctt tttcgcccat tatggcgagc gtgtagaagc ggaaatcatc tccccggaca
    17221 aaacgccaga cttgccaaac ttcgccgaaa tctttgcccg tcagagtagc tgggaatgga
    17281 acttcggtca ggctccggca ttctcgcatc tgctggatga acgctttagc tggggcggcg
    17341 tggaactgca tttcgacgtt gaaaaaggcc atatcacccg cgcccaggtg tttaccgaca
    17401 gcctcaaccc cgcgccgctg gaagccctcg ccgggcgact gcaaggctgc ctgtaccgcg
    17461 cggatatgct gcaacaagag tgcgaagcgc tgttggttga cttcccggac caggaaaaag
    17521 agctacggga gttgtcgacg tggatagcgg gggcggtaag gtaactcgct gattaaaata
    17581 ccaaaggaag ggaatatgat cgatctggaa aaccaggagc gggagattat caatttaatg
    17641 ttaagccagc gtatttcctg gctagccgcc gtgcgtatca ggcataaatt atcgcttgct
    17701 gaagtaagta aaatgttagg tatctcgatt aattctctaa aatagatcga aaagacggaa
    17761 cggtttagct caaacatcaa aagcaaaatg gcagaaattt atggttgtcc accagagttg
    17821 ctgatatgcc cttcctggat gacggctgaa cataaatgaa tagattcgtg aatagatact
    17881 tgaatagatt ttattttcta
//
LOCUS       DAJSKJ010000072        17589 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000072 DAJSKJ010000000
VERSION     DAJSKJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.072"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..1794)
                     /gene="espL1"
                     /locus_tag="ON345_002123"
     CDS             complement(<1..1794)
                     /gene="espL1"
                     /locus_tag="ON345_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR3072181.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINKFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKDII
                     SLLLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIHPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQ
                     LNYVVSVYDPNDTNVAVRDTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPILPSAMLLIMATGQTQQFITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANEIPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIA"
     gene            2318..4246
                     /gene="thrS"
                     /locus_tag="ON345_002124"
     CDS             2318..4246
                     /gene="thrS"
                     /locus_tag="ON345_002124"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR3072182.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4250..4792
                     /gene="infC"
                     /locus_tag="ON345_002125"
     CDS             4250..4792
                     /gene="infC"
                     /locus_tag="ON345_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR3072183.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4889..5086
                     /gene="rpmI"
                     /locus_tag="ON345_002126"
     CDS             4889..5086
                     /gene="rpmI"
                     /locus_tag="ON345_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR3072184.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5139..5495
                     /gene="rplT"
                     /locus_tag="ON345_002127"
     CDS             5139..5495
                     /gene="rplT"
                     /locus_tag="ON345_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR3072185.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKEALA"
     misc_feature    5612..5736
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5618..5662
                     /gene="pheM"
                     /locus_tag="ON345_002128"
     CDS             5618..5662
                     /gene="pheM"
                     /locus_tag="ON345_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR3072186.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5945..6928
                     /gene="pheS"
                     /locus_tag="ON345_002129"
     CDS             5945..6928
                     /gene="pheS"
                     /locus_tag="ON345_002129"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR3072187.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6943..9330
                     /gene="pheT"
                     /locus_tag="ON345_002130"
     CDS             6943..9330
                     /gene="pheT"
                     /locus_tag="ON345_002130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR3072188.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRKGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9335..9634
                     /gene="ihfA"
                     /locus_tag="ON345_002131"
     CDS             9335..9634
                     /gene="ihfA"
                     /locus_tag="ON345_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR3072189.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9735..10715
                     /gene="btuC"
                     /locus_tag="ON345_002132"
     CDS             9735..10715
                     /gene="btuC"
                     /locus_tag="ON345_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR3072190.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            10778..11329
                     /gene="btuE"
                     /locus_tag="ON345_002133"
     CDS             10778..11329
                     /gene="btuE"
                     /locus_tag="ON345_002133"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR3072191.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11329..12078
                     /gene="btuD"
                     /locus_tag="ON345_002134"
     CDS             11329..12078
                     /gene="btuD"
                     /locus_tag="ON345_002134"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR3072192.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12156..12620
                     /locus_tag="ON345_002135"
     CDS             12156..12620
                     /locus_tag="ON345_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3072193.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12867..13580
                     /gene="ydiV"
                     /locus_tag="ON345_002136"
     CDS             12867..13580
                     /gene="ydiV"
                     /locus_tag="ON345_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR3072194.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13643..15079
                     /locus_tag="ON345_002137"
     CDS             13643..15079
                     /locus_tag="ON345_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR3072195.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15083..15274)
                     /gene="hemP"
                     /locus_tag="ON345_002138"
     CDS             complement(15083..15274)
                     /gene="hemP"
                     /locus_tag="ON345_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR3072196.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLIRKTQAGKLLLTK"
     gene            complement(15406..16452)
                     /gene="aroH"
                     /locus_tag="ON345_002139"
     CDS             complement(15406..16452)
                     /gene="aroH"
                     /locus_tag="ON345_002139"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR3072197.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16609..17442)
                     /gene="ppsR"
                     /locus_tag="ON345_002140"
     CDS             complement(16609..17442)
                     /gene="ppsR"
                     /locus_tag="ON345_002140"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR3072198.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4476 a         4036 c         4590 g         4487 t
ORIGIN      
        1 ggctatatga acagctgaca tgttgtcatg atttttagcg gccaatagtg taaataactg
       61 atgttgacta aaagaatgtt tttttgcaaa agcacccagc gtcgatatat aagataacac
      121 gacgtcttca ttacccttgc tcatggcgat gtataatgca ggggttccct gtgctgtagc
      181 tccttttaat aaatccatga tgttagcttt gctcaacttg tatttaaagg cgatgccatt
      241 aattttagag aggaaccgtg tgacacacaa agggtgatta ttttccattg cggcgtataa
      301 tcctggctca ccatcatgta ttttcatggc taaaatactc atcaccagtt ttttgtttaa
      361 aagctcttgt gctattggag tggttaatgc attcagaaag atatcgatta tttcatcgaa
      421 tccatacaac atggagatat aaagtccagg tatttcgtta gcacttttag tttgcagtaa
      481 tttaaccaga ttatcttcat gaatgatgtg attatctacc agtgactgaa tttcttgcat
      541 aaatattttc acgttgtcag tatgtccgtt catcatagcc aaaaataaag caggtgtacc
      601 aacgctattc tgcgcagtta ttatttcaat gatttctttt tcagggagta tgggcaactg
      661 tttgaataat gtaataaact gttgagtctg gcctgtagcc attattaaca acattgccga
      721 tggaagtata ggggcaaatg gcattcgtga tgcaataccc gcgaaaatag cccctggaac
      781 tcccacaggt aataggggca gaatagcaat tgcacagttg cgaacatacc tatcagccca
      841 ggtctgagca tcgggacctg aactgatgaa cttatcaagc gaaagaaagc cccgtgctgt
      901 cctgtgggtg tctctgacgg caacgttggt atcgttcggg tcgtaaacgg acacaacata
      961 gtttaattgt ccatacactg actgctttat tcgtagcctt actgacaaag tatgattgtc
     1021 tatatataaa atcgcggaca tatgactgac attgtttgcc atcattcgac aaaagaaaat
     1081 atctaaagcg aagccaaaat ccttactcgc gataacgcga ctccctgcag gaggatggcg
     1141 aagtaatgct ctgaactccg cgtcccggac taaggggaca tgctgctgta tggattcaac
     1201 ggaggagtaa tgtttaatca ggttgacttt gccttttcca tttctcaatg aatcaagggc
     1261 gtattgcaat gacagatggc gacaaaaaat agcgtcagaa ggagattgca aaaaacatgc
     1321 actaccattg agatttactg ttgggtgtat agttttttct ctatggattt ttttagacag
     1381 tttcatacaa tggttacgat cttcagcagt taactgttcc tgttcaatat aatccagtat
     1441 gcttcgcaaa tggttagctt taataggcgt ggtttccagt aataaggaaa tgatatcttt
     1501 tttatagtgt aatgtaatga gtatattttc tgcatggatg ggtgaagtgg caaagttaat
     1561 ggtaaaaagg gcagtggaat aacaattttg tgatattttt cgacagtttg cggtaaattc
     1621 ctgagtaaca cagtatgaca gcatcgaact taagtcagag gtatcaagta gggtgatgag
     1681 ctggcgcgca aatttattaa taggctctga tgaaggtgtt ggtatgtcgc tatcgatgtg
     1741 caatctatga ggagacttat aatgagttct gataatgata tcgttttgac tcataatcta
     1801 ttcgccttat atatatctgg aggttttgag gatgatgatt aattattatc gccagaacga
     1861 aatgtgcacg aaaaattgtt ataagaattg tgtcactttt atcattgtga tatgaccact
     1921 tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa atcacgcaac gaactttgct
     1981 ggattcagtt tatgctgctg taaatccgct cgcagtaaac ctttcagacg caccgtgatg
     2041 ttatcagtgg ttctttaagc gttttgctgg tgtactcact acaaacgaat tgcgaatcga
     2101 atcaatgtga aacggaaagg tacaatctcc ctttttttag tgttaacatc gctcaaccgg
     2161 gtggaaggtg agaggttaat gtctttcatt tgtgtacctg attgcgaacc aatttagcat
     2221 ttgttggcta gatggtttcg caatgaactg ttaataaaca aatttttctt tgtatgtgat
     2281 ctttcgtgtg ggtcaccact gcaaataagg atataaaatg cctgttataa ctcttcctga
     2341 tggcagccaa cgccattacg accacgctgt aagccccatg gatgttgcgc tggacattgg
     2401 tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg ttgatgcttg
     2461 cgatctgatt gaaaacgacg cacaactgtc catcattacc gccaaagacg aagacggtct
     2521 ggagatcatt cgtcactcct gtgcgcacct gttagggcac gcgattaaac aactttggcc
     2581 gcataccaaa atggcaatcg gcccggttat tgacaacggt ttttattacg acgttgatct
     2641 tgaccgcacg ttaacccagg aagatgtcga agcactcgag aagcggatgc atgagcttgc
     2701 tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg cacgaagcgc gtgaaacttt
     2761 cgccaaccgt ggggagagct acaaagtctc cattcttgac gaaaacatcg cccatgatga
     2821 caagccaggt ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc cgcacgtacc
     2881 gaacatgcgt ttctgccatc atttcaaact aatgaaaacg gcaggggctt actggcgtgg
     2941 cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag acaaaaaagc
     3001 gcttaacgct tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc gtaaaatcgg
     3061 taaacagctc gacctgtacc atatgcagga agaagcgccg ggtatggtat tctggcacaa
     3121 cgacggctgg accatcttcc gtgaactgga agtgtttgtt cgttctaaac tgaaagagta
     3181 ccagtatcag gaagttaaag gtccgttcat gatggaccgt gtcctgtggg aaaaaaccgg
     3241 tcactgggac aactacaaag atgcaatgtt caccacgtct tctgagaacc gtgaatactg
     3301 cattaagccg atgaactgcc cgggtcacgt acaaattttc aaccaggggc tgaagtctta
     3361 tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc caccgtaatg agccgtcagg
     3421 ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag gatgacgcgc atatcttctg
     3481 tactgaagaa caaattcgcg atgaagttaa cggatgtatc cgtttagtct atgatatgta
     3541 cagcactttt ggcttcgaga agatcgtcgt caaactctcc actcgtcctg aaaaacgtat
     3601 tggcagtgat gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc tggaagaaaa
     3661 caacatcccg tttgaatatc aactgggtga aggcgctttc tacggtccga aaattgaatt
     3721 taccctgtat gactgcctcg atcgtgcatg gcagtgcggt acagtacagc tggacttctc
     3781 tttgccttct cgtctgagcg cttcttatgt aggcgaagac aatgaacgta aagtaccggt
     3841 aatgattcac cgcgcaattc tggggtcgat ggaacgtttc atcgggatcc tgaccgaaga
     3901 attcgctggt ttcttcccga cctggctcgc gccggttcag gttgttatca tgaatattac
     3961 cgattcgcag tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg cgggcattcg
     4021 tgttaaagca gacttgagaa atgagaagat tggctttaaa atccgcgagc acactttgcg
     4081 tcgcgtccca tatatgctgg tctgtggtga taaagaggtg gaatcaggca aagttgccgt
     4141 tcgcacccgc cgtggtaaag acctgggaag catggacgta aatgaagtga tcgagaagct
     4201 gcaacaagag attcgcagcc gcagtcttaa acaattggag gaataaggta ttaaaggcgg
     4261 aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg cccaggaagt
     4321 tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag aagctctgga
     4381 gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg agccgccggt
     4441 ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt ctaaggaaca
     4501 gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg gtacagatga
     4561 aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg gtgataaagc
     4621 caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg gtatggaagt
     4681 gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct tcccaacgaa
     4741 gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagt aaggccttca
     4801 agtagcactg tctgtggagc cttcgggctt cacaggcttt gttcgccttt gtttcgttta
     4861 ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt aagaccgtac gcggtgctgc
     4921 taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta acctgcgtca
     4981 cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag ccatggtttc
     5041 caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac gcataagccg ttaacgtttt
     5101 taacttttta attagaatat agatacagga gagcacatat ggctcgcgta aaacgtggtg
     5161 ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc taaaggctac tacggtgcgc
     5221 gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa agctggtcag tatgcttacc
     5281 gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg gattgcgcgt atcaacgcag
     5341 cagcacgtca gaacggtatt tcttacagca aattcatcaa tggcctgaaa aaagcctctg
     5401 ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt cgacaaagta gcgttcaccg
     5461 ctctggttga aaaagcgaaa gaagctctgg cataagccag ttgaaagagg gagctagtct
     5521 ccctcttttc gtttcaacgc catcaaaaca ttgactttta tcgccgtagc cttttcaata
     5581 aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta ttttccgctt
     5641 ctttttttac tttagcacct gaatccagga ggctagcgcg tgagaagaga aacggaaaac
     5701 agcgcctgaa agcctcccag tggaggcttt ttttgtatgc gcgttttgaa aaataaaaag
     5761 ccagtctatc aggcgtaatt gtcgcagcca gtctggacac ggccagcgcg gagaaaccgg
     5821 agcgtattta agtacgtgag aatttcgagc actgcccggg accaaaatgg caagtaaaat
     5881 agcctgatgg gataggctct aagtccaacg aaccagtgtc accactgaca caatgaggaa
     5941 aaccatgtca catctcgcag aactggttgc cagtgcaaag gcggccatta gccaggcgtc
     6001 agatgttgcc gcgttagata atgtgcgcgt cgaatatttg ggtaaaaaag ggcacttaac
     6061 ccttcagatg acgaccctgc gtgagctgcc gccagaagag cgtccggcag ctggtgcggt
     6121 tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat gcgcgtaaag cggaactgga
     6181 aagcgcagcg ctgaatgcgc gtctggcggc ggaaacgatt gatgtctccc tgccaggtcg
     6241 tcgcattgaa aacggcggtc tgcatccggt tacccgtacc atcgaccgta tcgaaagttt
     6301 cttcggtgag cttggcttta ccgtggcaac cgggccggaa atcgaagacg attatcataa
     6361 cttcgatgct ctgaacattc ctggtcacca cccggcgcgc gctgaccacg acactttctg
     6421 gtttgacact acccgcctgc tgcgtaccca gacctctggc gtacagatcc gcaccatgaa
     6481 agcccagcag ccaccgattc gtatcatcgc gcctggccgc gtttatcgta acgactacga
     6541 ccagactcac acgccgatgt tccatcagat ggaaggtctg attgttgata ccaacatcag
     6601 ctttaccaac ctgaaaggca ctctgcacga tttcctgcgt aacttctttg aggaagattt
     6661 gcagattcgc ttccgtcctt cctacttccc gttcaccgag ccttctgcag aagttgacgt
     6721 catgggtaaa aacggtaaat ggctggaagt gctgggttgc gggatggtgc atccgaacgt
     6781 gctgcgtaac gttggcatcg acccggaagt ttactctggt ttcgccttcg ggatggggat
     6841 ggagcgtctg actatgttgc gttacggcgt caccgacctg cgttcattct tcgaaaacga
     6901 tctgcgtttc ctcaaacagt ttaaataagg caggaataga ttatgaaatt cagtgaactg
     6961 tggttacgcg aatgggtgaa cccggcgatt gatagcgatg cgctggcgaa tcaaatcact
     7021 atggcgggcc tggaagttga cggtgtagaa ccggttgccg gtagcttcca cggcgtggtc
     7081 gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg acaaactgcg tgtgacaaaa
     7141 gtgaatgtcg gcggcgatcg cctgctggac atcgtctgtg gtgcgccaaa ctgccgtaag
     7201 ggcctgcgtg tggcggtagc taccattggt gctgttctgc cgggtgattt caaaattaaa
     7261 gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt gctccttctc tgaactggga
     7321 atttctgacg atcacagcgg cattatcgaa ctgcctgcag atgcgccgat tggcactgac
     7381 atccgcgaat acctgaaact cgatgacaac accatcgaaa tcagcgtaac gccaaaccgt
     7441 gccgactgct taggtatcat tggtgttgcg cgtgacgttg ccgtgctgaa tcagctgccg
     7501 ctggttgaac cggaaatcgt tccggttggt gcgaccatcg acgacacgtt gccgattaca
     7561 gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg tggtaaaagg cattaacgtt
     7621 aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc gttgcgggat ccgttctatc
     7681 gatgcagttg ttgacgtcac caactatgta ctgctcgaac tgggccagcc gatgcacgct
     7741 ttcgataaag atcgcattga aggcggcatt gtggtgcgga tggcgaaaga gggcgaaacg
     7801 ttggtactgc ttgatggcac tgaagcgaag ctgaatgctg acactctggt catcgccgac
     7861 cacaacaagg cgctggcgat gggcggcatc ttcggtggtg aacactctgg cgtgaatgac
     7921 gaaacacaaa acgtgctgct ggaatgcgct ttctttagcc cgctgtctat caccggtcgt
     7981 gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt atgagcgtgg cgttgatccg
     8041 gcactgcagt acaaagcgat ggaacgtgcg acccgtctgc tgatcgacat ctgcggtggt
     8101 gaggctggtc cggtaattga tatcaccagc gaagcaacgc tgccgaagcg tgcaaccatc
     8161 actttacgcc gtagcaaact ggatcgcctg atcggccatc atattgcgga tgagcaggta
     8221 actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag gcaaagacga gtggcaggca
     8281 gttgcgccga gctggcgttt cgacatggag attgaagaag atctggtcga agaagtcgcg
     8341 cgagtttacg gctacaacaa catcccggat gagccggtac aggcaagcct gattatgggt
     8401 actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa cgctgctcaa cgacaaaggc
     8461 tatcaggaag tgatcaccta cagcttcgtt gatccgaaag tgcagcagat gatccatcca
     8521 ggcgttgaag ccttactgct gccaagcccg atctctgttg aaatgtcagc aatgcgtctt
     8581 tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc agaaccgtca gcagaaccgt
     8641 gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata ctcaggcacc gttgggcatt
     8701 cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc gttacgaaga gcactggaac
     8761 ctggcaaaag agaccgttga tttctatgat ttgaaaggcg atcttgaatc cgttctcgac
     8821 ctgaccggta aactgaatga ggttgagttc cgtgcagaag cgaatccggc actgcatccg
     8881 gggcaatccg cagcgattta tctgaaaggt gaacgtattg gttttgttgg ggttgttcat
     8941 cctgaactgg aacgtaaact ggatcttaac ggtcgcactc tggtgttcga actggagtgg
     9001 aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga tttctcgctt cccggcgaac
     9061 cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg cagcggatat tttatccgaa
     9121 tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact tatttgacgt gtaccgcggt
     9181 aagggtgttg cggaggggta taagagcctc gccataagcc tgatcctgca agataccagc
     9241 cgtacactcg aagaagagga gattgccgct accgtcgcca aatgtgtaga ggcattaaaa
     9301 gagcgattcc aggcatcatt gagggattga acctatggcg cttacaaaag ctgaaatgtc
     9361 agaatatctg tttgataagc ttgggcttag caagcgggat gccaaagaac tggttgaact
     9421 gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa caggtgaaac tctctggttt
     9481 tggtaacttc gatctgcgtg ataagaatca acgcccggga cgtaacccga aaacgggcga
     9541 ggatattccc attacagcac ggcgcgtggt gaccttcaga cccgggcaga agttaaaaag
     9601 ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat ctaactaaaa aggccgctct
     9661 gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa atcaacgcca tgacacttca
     9721 gcagaacgga taccatgctg acacttgccc gccaacaaca gcgacaaaat attcgctggt
     9781 tattatgcct gtcagttttg atgctgctgg cgcttctctt aagcctttgc gccggtgaac
     9841 aatggatctc gccaggtgac tggtttactc ctcgtggcga actgttcgtc tggcaaattc
     9901 gcctgccacg tacgctggct gtattgctgg ttggtgcggc gctggctata tccggcgctg
     9961 taatgcaggc gttgtttgaa aatcctctgg cagaacctgg actacttggc gtctctaacg
    10021 gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca agggcaactc cccaactggg
    10081 cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac tttaatactc ttacgtttcg
    10141 cccgtcgtca tctttcgacc agtcggttat tgctggctgg cgttgcatta gggattatct
    10201 gtagcgcact aatgacgtgg gctatctact tttccacatc tgttgattta cgtcagctga
    10261 tgtactggat gatgggcggt tttggcggcg tagactggcg gcaaagctgg ctgatgctgg
    10321 cattgatccc cgtgttgttg tggatctgtt gtcagtccag gccgatgaat atgttagcac
    10381 ttggcgagat ctcggcgcgg caactgggtt tacccctgtg gttctggcgc aatgtgctgg
    10441 tggccgcgac cggctggatg gttggcgtca gtgtggcgct ggcgggtgct atcggcttta
    10501 ttggtctggt gatcccacat attcttcggt tgtgtggttt aaccgatcat cgcgtattac
    10561 ttcccggctg cgcgctggca ggggcgagcg cattgctgct ggccgatatt gtagcgcgcc
    10621 tggcattagc tgccgcagag ctgcctattg gcgtggtcac cgcaacgttg ggtgcgccgg
    10681 tgtttatctg gttattgtta aaagcaagac gttagccgca aaaagacggt ctatgattaa
    10741 aagctaagat ttttactgac cacacccagg agaaacgatg caagattcca ttctgacgac
    10801 cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag aagtacgccg gtaatgtgct
    10861 gttgattgtc aatgtcgcct caaagtgtgg cttaacgccg caatatgagc agttggagaa
    10921 tattcagaaa gcctgggccg atcgaggttt tgtggtgctg ggattcccgt gcaaccagtt
    10981 tctggaacaa gaaccgggca gcgatgaaga gattaaaact tactgtacca ccacatgggg
    11041 ggtgacgttc ccgatgttca gtaagattga agttaatggc gaaggacgcc atccgctgta
    11101 tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg gaagagagcg gattctatgc
    11161 ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat gatattttat ggaattttga
    11221 aaaattcctg gttggcaggg acggaaaagt catccagcgt ttttccccgg atatgacgcc
    11281 ggaagatccc attgtgatgg aaagcattaa actggcgtta gcaaaataat gtctattgtg
    11341 atgcagttac aggatgttgc ggaatctacc cgcctggggc cgctttctgg cgaggttcgg
    11401 gctggggaga tcctgcacct ggtggggccg aatggcgcgg gtaagagtac cttgcttgca
    11461 cgaatggccg gaatgaccag cggtaaggga agcattcagt tcgcggggca acccctggaa
    11521 gcatggtccg caacaaaact cgcgctgcat cgcgcctatc tttcacaaca gcagacgccg
    11581 ccgtttgcaa tgccggtctg gcactacctg acactgcatc agcacgataa aacgcgtacc
    11641 gaactactga atgatgtcgc cggggcgctg gctcttgatg acaaactcgg tcgtagcacc
    11701 aatcaacttt ccggcggtga atggcaacgc gtacgtcttg ctgcggtggt gttgcaaatc
    11761 acaccacaag ccaatcccgc aggccaattg ctgcttcttg atgagccgat gaacagtctt
    11821 gatgttgcgc aacaaagtgc gttagacaaa attctgagcg cgctgtgtca gcaaggactg
    11881 gcgattgtga tgagcagtca cgatctcaac cacacgttgc gtcatgcgca tcgggcgtgg
    11941 ttgctaaaag gtggaaaaat gctggccagt ggacgcagag aagaggtgct cacgccgcca
    12001 aatctggcgc aggcctatgg gatgaatttt cgccgtctgg atatcgaagg tcacagaatg
    12061 ctgatttcga ccatctgatt tcattagtat cttgaaaatc gggtaattac gacgctaaat
    12121 taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg tttctgcctt attttgatca
    12181 cagcactgtt tctggccggg tgtagccacc ataaagcacc gccgccaaat gccagacttt
    12241 ctgattcgat taccgttatt gccggtttga acgaccagct acaaagctgg catggcacgc
    12301 cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg ttcgggattt gtggttgtga
    12361 cgatgcgcga tcgtttcgat ttgcagctgc cccgtgaaac caaacaacaa gcctctattg
    12421 gcacgcaaat tgataaagac gagttgctgc caggtgacct ggtctttttc aaaacggggt
    12481 ccggacaaaa tggtttgcat gtaggtattt acgataccaa caaccaattt atccacgcct
    12541 ctaccagcaa gggagtgatg cgttcctcac ttgataatgt ctattggcag aaaaatttct
    12601 ggcaggcgag acgaatctag tatgtcgaaa aatggacggc gatgatacat cgccgtccat
    12661 ttactcctga atacagtagt attttgcaga caaaaattcc gtaaatggat tattcgttta
    12721 taatcaggat aatctaaaaa aaggacccct gaaaaagtgg ttaacatgga acaaatgaaa
    12781 gttactcctt ctgtaattgt tatgtcttat ggcctcatcc cttttagccg gatactgaaa
    12841 aacatccttc gagagggacg gttaccatga agattttttt ggagaatctt tatcattctg
    12901 attgctattt cctgccgata agagataacc agcaagtcct tgtcggcgtt gagttaatta
    12961 cccatttctc cagtgaagat ggcacggtgc gtatcccgac gagtcgggtt atagcgcaac
    13021 ttaccgagga gcaacattgg caattatttt cagagcagtt ggaattacta aaatcatgcc
    13081 aacatttttt tattcaacat aaactctttg cctggcttaa tttaacgcca caagtggcta
    13141 cattattatt agagagggat aattacgcag gcgaactatt aaaatatccg tttattgaac
    13201 tgttgattaa tgagaattac ccgcatctca atgaaggaaa agataatcgt ggtcttttat
    13261 ccttatcgca agtgtatccc cttgtgttag gtaatttggg agcaggcaat agcacaatga
    13321 aagcggtttt tgatggtctt ttcacccgcg tcatgctgga taaaagtttt attcagcaac
    13381 aaatcacgca ccgctccttc gaaccgttta ttcgtgcgat ccaggcgcaa atttcccctt
    13441 gttgcaattg catcattgcg ggaggcattg atacggcaga aatattggca cagattaccc
    13501 ccttcgattt ccatgctctc cagggatgcc tgtggcctgc cgttcccata aatcagataa
    13561 cgacgttggt tcagcgataa cccttctgtt tgctggtgtt taagacgaga gtaaccgtct
    13621 acactatcaa acaggaggat ctatgaccct gtcttttgtt acccgctggc gcgatgaatt
    13681 gccagaaacc tatacagcac tttcccctac gcctttaaat aatgcccggc tgatttggca
    13741 taataccgaa ctggctaaca cgctgagtat tccatcgtcg ctgtttaaaa atggcgcagg
    13801 tgtctggggc ggcgaagccc tactgcctgg tatgtcacca ctggcgcagg tttacagtgg
    13861 tcatcagttc ggcgtctggg cgggccaact gggtgatggg cgcggcattt tactcggcga
    13921 acaactgctt gctgatggca ctacaatgga ctggcatctg aaaggtgctg gcctgacgcc
    13981 ttattcgcga atgggtgatg gacgggcggt tttacgttcg acgatacgag aaagtctcgc
    14041 cagtgaggcg atgcattatc tgggcattcc gacgacccgc gcgttaagta tcgtcaccag
    14101 cgattcgcca gtgtatcggg aaacggcgga gccaggcgcg atgctgatgc gtgtggcacc
    14161 aagtcatctg cgctttggtc atttcgaaca tttttactac cgccgcgagt cggaaaaggt
    14221 tcgtcagtta gctgactttg ctattcgtca ttactggtca catcttgaag atgatgagga
    14281 caaataccgt ctctggttta gcgatgttgt cgcacgtacc gcgtcgttaa ttgcccaatg
    14341 gcagacggtc ggctttgcac atggggtgat gaataccgac aacatgtcgc tgctgggact
    14401 gacgcttgat tacgggccgt ttggttttct tgatgattac gaacccggtt ttatttgtaa
    14461 tcactcggat catcaagggc gttatagctt tgataatcaa cctgctgtcg cgttgtggaa
    14521 tttacagcgt ctggcgcaga cattgtcacc atttgttgcc gtagatgccc tgaatgaggc
    14581 cctggacagc tatcagcagg ttttgttgac gcattatgga caacggatgc ggcagaaact
    14641 gggcttcatg acggagcaaa aagaggataa cgcgctactg aatgaattat tcagtctgat
    14701 ggcgcgagag cgcagcgatt atacccgcac attccgcatg ctgagtctga ccgagcagca
    14761 cagcgcggcg tcaccgctac gtgatgagtt tattgatcgt gcggcatttg atgactggtt
    14821 tgcccgttat cggaggcgtt tgcaacaaga cgaggttagc gatagtgagc gtcagcagct
    14881 gatgcaaagc gttaacccag ctctggtgtt gcgcaactgg ttggcgcaac gggcgattga
    14941 agcggcagaa aagggtgata tgacggaatt gcaccgcctg catggggcgt tgcgaaatcc
    15001 tttcagcgat agagatgatg actatgtcag tcgtccacct gactggggta aacggctgga
    15061 agtcagctgc tcgagttaaa ggctacttag ttaacagcag cttgccagcc tgagttttac
    15121 ggatcagata ttcttgcccg tcatgatcga taatcagttt gccatccgga cctaacagtg
    15181 tctggctggt aattcgccgg atgggctgcg gggaagcggc cttgtgattg gcgtccgttt
    15241 caggcgtgag ttttctgcta tccgtataac gcataaagat taataataat tgatataaca
    15301 atgattctta ttatcaatat tatggcagac cagagcaagc agtaatttgt tgagaagaca
    15361 acaagttgcg cagaaatgcc cattccgtca ggaatgggca cgcattcaga agcgggtatc
    15421 taccgcagag gcgagttttt cgaccaggcg ttcggtatct tcccagccca gacacgggtc
    15481 ggtaatggat tgaccgtaag tgagcggctg actgccgacg attttttgcg ttccttcgcg
    15541 caggaaactt tccgccataa ttccagcaat cgccgtagag ccattgcgga tttgctgaca
    15601 aatatcctca caaacttcta actggcgacg gtgctgcttc tggcagttac cgtggctgaa
    15661 atccaccacc agatgttcag gtaaatcaaa ctcgtgcagc gtatcgcagg ctgcggcgat
    15721 atcatcggca tgataattcg gttttttgcc gccacgcata ataatgtggc catacgggtt
    15781 gccgctggtc tgatagatgg tcatctgacc atttttgtct ggcgagagga acatatggct
    15841 ggcgcgggct gcgcggatag catccacagc aatccgcgta ttgccatcgg taccattttt
    15901 aaaacctacc ggacaggaga gtgccgaagc catttcacgg tggatctgac tttcggtagt
    15961 acgtgcgcca atcgcgcccc aactgattaa atcagcaata aactgaccgg tcaccatatc
    16021 gaggaactca gtcgcggttg ggacgcccag ctcatttacc tgtaaaagta atttgcgcgc
    16081 cagctccaga ccgtgattta cccgatagct gccgtttaaa tccggatcgg agattagccc
    16141 tttccagccg acaacagttc gtggtttttc aaaataggtg cgcattacga tttccagccg
    16201 tgactggtac tggttgcgca gcgactgcag acgggtggcg tactccattg cagcggtgag
    16261 atcgtggatc gagcaggggc caataatgac caacagtcgc ttatcttcac cattcagtat
    16321 tttttcaatt ctgcggcggg agtcggtgac atgggtggcg acgccaggcg ttacgggata
    16381 ccgtagcgcg agttcggcgg gcgttaccag gctctcaata cgcgcagtac ggagttcgtc
    16441 agttctgttc attacaagtc tcagaaattt gttgcttcta cggcaggact accgggaagt
    16501 gatgcgcatc acgataaacc aatcgcagct gatttcaagt gcgaggtgtg tcacacctcg
    16561 ccgggtggtt agcatgataa caaaaaaaca aaccaatgca ctagttctct agtacattcg
    16621 gcgactaagg cccatgatat cgaggatctt ggtggcaatc tcttctaccg aataattggt
    16681 actgttaatc cacgggatct gatttttacg gtacaaggct tccacttccg cgacttccat
    16741 tctgcactga cgaagcgagg catagcgact gttctcccga cgttcctcgc gaatcgccgc
    16801 cagacgttcc gggtcgatag tcaggccgaa caatttatgc tgaagcggtt tgagcgacgc
    16861 gggtagcacc agattatcca tatcgtcggc aataaagggg tagtttgccg cgcggatacc
    16921 aaattgcatt gccagataca gactggtggg ggttttacca cagcgagaaa cgccgagcag
    16981 gatcacctga gcctggtcca gattgcgcaa cgaaataccg tcatcgtggg caagggtgta
    17041 atcaatcgcc gcaatgcgcg catcatattt attgagatta ttagggttaa ggccatgggt
    17101 acgatgagca atcggcgttg gatccagttt catctcttgt tgtagcgggg caaccagcgc
    17161 ctgaacgata tcctggcaaa agccttcact ttgcaagatg atggcgcgaa tctccggcaa
    17221 cacgatggag tagaagacca gcgggcgcac acctgtctgg tgataaattg cgtcaatctg
    17281 atccttcact gcccgtgcac ggctctcatt ttcgacaaac ggcagcgtaa tgctgctgat
    17341 agtgacggga aattgtgaca ttactgcgtg tcctaatacc tccgcagtta ttgccgtacc
    17401 atcagaaata taaaaaacgt ggcgatcaac agcattatcc attttgttct tcccgtgatg
    17461 cagacataat tctatgaaag cataaattaa aaacgcacag aagcgtagaa cgttatgtct
    17521 ggtttataaa atgaaccttc aattttattt tttatgaaaa cagcatttca tttttatggt
    17581 ttcgtttat
//
LOCUS       DAJSKJ010000073        17562 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000073 DAJSKJ010000000
VERSION     DAJSKJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.073"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..223
                     /locus_tag="ON345_002141"
     CDS             <1..223
                     /locus_tag="ON345_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR3072199.1"
                     /translation="FDNREINEETVCYAVALKQRNKTLAAISISIPVFRATKEKIADV
                     TRILLNARSRIEQELNTLQDINIGIDSHH"
     gene            complement(300..1031)
                     /gene="rlmB"
                     /locus_tag="ON345_002142"
     CDS             complement(300..1031)
                     /gene="rlmB"
                     /locus_tag="ON345_002142"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR3072200.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1122..3563)
                     /gene="rnr"
                     /locus_tag="ON345_002143"
     CDS             complement(1122..3563)
                     /gene="rnr"
                     /locus_tag="ON345_002143"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR3072201.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSVKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3602..4027)
                     /gene="nsrR"
                     /locus_tag="ON345_002144"
     CDS             complement(3602..4027)
                     /gene="nsrR"
                     /locus_tag="ON345_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR3072202.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4232..5530)
                     /gene="purA"
                     /locus_tag="ON345_002145"
     CDS             complement(4232..5530)
                     /gene="purA"
                     /locus_tag="ON345_002145"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR3072203.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5634..5831)
                     /locus_tag="ON345_002146"
     CDS             complement(5634..5831)
                     /locus_tag="ON345_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR3072204.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5913..6917)
                     /gene="hflC"
                     /locus_tag="ON345_002147"
     CDS             complement(5913..6917)
                     /gene="hflC"
                     /locus_tag="ON345_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR3072205.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6920..8179)
                     /gene="hflK"
                     /locus_tag="ON345_002148"
     CDS             complement(6920..8179)
                     /gene="hflK"
                     /locus_tag="ON345_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR3072206.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8265..9545)
                     /gene="hflX"
                     /locus_tag="ON345_002149"
     CDS             complement(8265..9545)
                     /gene="hflX"
                     /locus_tag="ON345_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR3072207.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLS"
     gene            complement(9622..9930)
                     /gene="hfq"
                     /locus_tag="ON345_002150"
     CDS             complement(9622..9930)
                     /gene="hfq"
                     /locus_tag="ON345_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR3072208.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10016..10966)
                     /gene="miaA"
                     /locus_tag="ON345_002151"
     CDS             complement(10016..10966)
                     /gene="miaA"
                     /locus_tag="ON345_002151"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR3072209.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10959..12806)
                     /gene="mutL"
                     /locus_tag="ON345_002152"
     CDS             complement(10959..12806)
                     /gene="mutL"
                     /locus_tag="ON345_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR3072210.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRRF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPSKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAASWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12816..14153)
                     /gene="amiB"
                     /locus_tag="ON345_002153"
     CDS             complement(12816..14153)
                     /gene="amiB"
                     /locus_tag="ON345_002153"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR3072211.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14172..14633)
                     /gene="tsaE"
                     /locus_tag="ON345_002154"
     CDS             complement(14172..14633)
                     /gene="tsaE"
                     /locus_tag="ON345_002154"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR3072212.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14605..16152)
                     /gene="nnr"
                     /locus_tag="ON345_002155"
     CDS             complement(14605..16152)
                     /gene="nnr"
                     /locus_tag="ON345_002155"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR3072213.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16151..17290
                     /gene="queG"
                     /locus_tag="ON345_002156"
     CDS             16151..17290
                     /gene="queG"
                     /locus_tag="ON345_002156"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR3072214.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17273..17326)
                     /locus_tag="ON345_002157"
     CDS             complement(17273..17326)
                     /locus_tag="ON345_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072215.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3993 a         5001 c         4413 g         4155 t
ORIGIN      
        1 atttgataac agggaaatca atgaggagac ggtatgttat gctgttgcct taaaacagcg
       61 aaataaaacg cttgccgcca tcagcatttc aatacccgta tttcgtgcga caaaagaaaa
      121 aattgccgat gtcacgcgta tcctgctgaa tgcacgttcg cgtattgaac aggaactcaa
      181 caccttacag gatatcaata tcggtattga ttcccaccat taagggtatt ttccattcga
      241 agcccgatac gtaaaaatta tcgggctaac aaaatatcca ttgaatggat agcgatgaac
      301 tagctgcgct ggcgcaccgc ttcaaataag caaattccgg tcgcaaccga aacgttcagg
      361 gaagaaacac ttcctgccat tgggatgctg atcaactcat cgcaatgttc acgggtcagg
      421 cgacgcatac cttcaccttc cgcgcccatc accagcgcca ggcgtccggt cattttgctc
      481 tgatagagtg tatgatctgc ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc
      541 aacatacgca tggtgcgcgc aaggttagtc acccgaatca gtggaacgct ttctgccgcg
      601 ccgcaggcta cttttttcgc cgtagcgttg agctgtgcgg agcgatcttt cggcacaatc
      661 accgcgtgaa cgccagcagc gtccgcgcta cgcaggcacg cgccgaggtt gtgcggatcg
      721 gttacaccgt cgaggatcag caggaacggt tgatcgagcg aagcgatcag atccggcaga
      781 tcgttttcct gatactgacg tcctggcttc acgcgggcga taatgccctg atgcacggca
      841 ccgtcgcttt tctcgtcgag atattggcgg tttgccaact ggataaccac gccctgagac
      901 tcaagggcgt gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact
      961 tcctgaaaac gttcaggggc gcgctccagc agggcctgca ctgcgtggat gccgtaaatc
     1021 atttcgctca ttaatgtact cgttatttac gttttgccgg gagcgccacg cttgcccggc
     1081 ctacggataa ttcaccctct tctgtttgaa gagggtaatg atcactctgc cacttttttc
     1141 ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcac cgatggcttt
     1201 ttcgcttttc tcgcgtcttt cttcgctgct ttcggcttcg gttttttctc gccgcggaag
     1261 gcgctgtctg gctcaaagtt gacctttttg ccgacctgac gccgcttgcc gccttttttg
     1321 cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt
     1381 tcgctggaga tcaggctaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga
     1441 acttccacgc gatcgcccag gcgataagtc tggccactgg attcacccat caggcgttgc
     1501 cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca
     1561 atgaacaagt cgtccaggcg aacaaagaag ccaaaaccag tgacgctgga aattacgcct
     1621 ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc agccacatcg
     1681 cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcagcatc
     1741 tcttccatcg aataatggta gccgccggtt tcagtggtgt taccctgatg cccttgctct
     1801 ttcgccagca gatacttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc
     1861 gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttccggatcg
     1921 taaatcgcct gtttcatcga gcgcagcagc atggtttgca gcatttctgc gtcaggacga
     1981 tcggcaaccg actccagcag ctctgcgtag tcgcgcggtt ccggcttgtt accgcccggc
     2041 agttccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc
     2101 ttatcgtgaa tacggaatag tgctggttct ttggctttct caacgaaacg cgccgccgag
     2161 atattcgcca gaatcatgca ctcttcaatt aacttgtgtg cgtcgttacg ctgggtctgt
     2221 tcgatacgct caatacggcg ttcggcgttg aaaatgaact tcgcttcttc gctctcaaat
     2281 gagatcccgc cacgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct
     2341 tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata
     2401 tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc gtagaatttg
     2461 tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct
     2521 acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat cgggataacc
     2581 tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag cggcgttggc
     2641 ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc gccgccgcgt
     2701 tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc
     2761 agcggtaaat cgcgcagatc aacacggccc gcttttgctt cttccggcac ttcttctttc
     2821 agcccggcaa cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc
     2881 agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc gacgatttta
     2941 cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac cacaaagccc
     3001 atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagcttaa acgactgtcg
     3061 tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt
     3121 tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcacccagc
     3181 ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac
     3241 aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg gccaataacg
     3301 gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg agtgaagacc
     3361 agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg
     3421 ccttcaatgt gcagttctac cgccaactca tcacggctgg ccggtttttc acgtttggtt
     3481 aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt
     3541 tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc
     3601 gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg
     3661 tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc
     3721 gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca
     3781 gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg cgaatgccgc
     3841 cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga
     3901 ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc
     3961 cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta
     4021 actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg atgataaacc
     4081 agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa
     4141 cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg
     4201 gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacggatcg cgcagaatca
     4261 tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg gtcagctctt
     4321 caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct ttcacgccga
     4381 aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt
     4441 cagctgccag cggagtggtc gtcacttcac gaccatccgg catacggtaa gccacacaga
     4501 gtttcacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacaggg
     4561 agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac
     4621 gacccgtagt tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa
     4681 acagttcggt cgggaaagga cctgcaccta cacgagttga gtaagctttg aggataccca
     4741 gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg
     4801 tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc gtaccctgcg
     4861 caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt
     4921 caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt
     4981 aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt
     5041 ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac
     5101 gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg atcgctttcg
     5161 cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac
     5221 atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca
     5281 tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg ctggttacat
     5341 tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga
     5401 gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca
     5461 gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt
     5521 tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca
     5581 gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca
     5641 atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa
     5701 cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc
     5761 gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt
     5821 gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg
     5881 tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc
     5941 atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag
     6001 ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc tttactgaat
     6061 gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcatgat gcggccctga
     6121 cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc
     6181 gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata
     6241 cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat acgcacatcg
     6301 acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc
     6361 gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatccgctgc cggggtagta
     6421 acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga
     6481 cgaccacggg aatcggtgac gatgtctttc acgtccaggc gaccaatttc agaacgcaga
     6541 cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt
     6601 gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagaatc gacgatcagg
     6661 tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg
     6721 tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagacccgg ctcataaacc
     6781 agaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca
     6841 ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg
     6901 ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg
     6961 cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg
     7021 ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg
     7081 ccgcaggggc gttaccgcct ttcagcatct ggtctaacgg cagaaccatc aggttgccac
     7141 ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc gtctcgatat
     7201 acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc
     7261 gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga
     7321 ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt
     7381 cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta
     7441 cttcttccgg cggacgagca gcctggaagt tgacgtccag cagagtgatg cccatgtcat
     7501 acggacgaat cgtctcttcc agttcgcgct gggtatcgct acgaatcacg gtacgacctt
     7561 ccgtcagaat gcggtccatg gtgtatttac cgataacccc gcgcagggcg ctgtcggtag
     7621 cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc ggattggtaa
     7681 cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac
     7741 cggaagcggc cagttcacgc acggcttcca cgtttaccgg tttgacttcg tcgataaacg
     7801 tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca
     7861 cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg
     7921 ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac
     7981 tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt
     8041 tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc
     8101 cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac
     8161 cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct
     8221 ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagct caggtaatcg
     8281 atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc
     8341 agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa
     8401 cgacttctca gacgcccttc ctgcggcggc aaacgcaatg tatgctgcgc cacctcgccg
     8461 gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt ctgtgcggaa
     8521 agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc
     8581 agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca
     8641 agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg
     8701 tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga
     8761 tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc accgacatcc
     8821 gcgacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag
     8881 acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc
     8941 agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt
     9001 tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg
     9061 gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg
     9121 tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc
     9181 aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg
     9241 tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca
     9301 tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct
     9361 ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc
     9421 aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct
     9481 ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca
     9541 aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg
     9601 taaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg cggaagtatt
     9661 ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt tgttactgtg
     9721 atgagaaacc gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct
     9781 gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc cttgcagctt
     9841 aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa
     9901 cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc ttatttgtac
     9961 tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta cacattcagc
    10021 ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt ttttcactat
    10081 caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc gccaactgtc
    10141 tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct tcaaggtaag
    10201 accacatctg gcgataaccc acgcaacgaa tggaaggcaa atccgtatgc aaatctcctc
    10261 gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga tgaaaacgct
    10321 gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac tgatgcacct
    10381 gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta cccgaaatga
    10441 aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc cttgctgccg
    10501 caaccggatc gatctcctga agttgacgat gcaatgactc ccaaccttgc tctgccgcct
    10561 gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac aacccttcca
    10621 gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc cccgccgcgg
    10681 tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg
    10741 acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca gcgttcggct
    10801 tcgccgtccc gatatccatc cctttgtaaa taagggcaga atcaacgctt atcaactcta
    10861 ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc gtcggcccca
    10921 tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt cagggctttt
    10981 atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc
    11041 ggacataacc gttccacgtc cgccaacagg gttatggcct gtgccattga ccactgcgca
    11101 tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac
    11161 tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag
    11221 ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat
    11281 tccgccaggg cagactgcgc tttttctaat gccgattttt cttcgccaga aacttttagc
    11341 cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc
    11401 tgacgtagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc atcgcgctcc
    11461 agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc
    11521 gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc gggcgtttgc
    11581 aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag
    11641 gaggcagccg gacgactgcc tgatgctggc gcaggagtgt aacgcggagc taccggctcc
    11701 cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc cggaatggca
    11761 cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg ctgctgtaac
    11821 acgctcagca cgccttgata gataaagtca tgcaccagac gcgactgatg aaaacgcact
    11881 tcgtgtttgg aggggtgcac gttgacgtcc acctgatgtg ggtcgatctc cagatacaac
    11941 acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcgtga
    12001 ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat ttccgccagt
    12061 gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc gccgtgttgc
    12121 cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccac aaatcgcgcc taagcgccgt
    12181 tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac
    12241 aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc atcgatatgg
    12301 ttaaattcgg ttttctcggt gcgcaggaat ctgcgccgcg ccggggtgtt gtagaacaga
    12361 tccagcacct ccagcgtcgt ccccacagga tgcgccgccg gttttaccgt cacgttcata
    12421 tcgcgccctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc
    12481 gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata
    12541 atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc
    12601 gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca
    12661 ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt ttccaccagt
    12721 tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt
    12781 tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt gcgatctggc
    12841 gtcgtcaccg tactggcagt ttgtgccgtt gccccctgcg gcgcagattg catcggatgc
    12901 gcaaggaaat aattgcgcag gcctttgtaa atggcttctg ccagctgttg ttggtaatcg
    12961 tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc gaccagtact
    13021 gacgggatat ccggcgaacg cagaacgccg agactcgcgt gttctggtcg gcgtttatgt
    13081 atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata ccctacccgt
    13141 tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc agactgactg
    13201 ttcgccagca catcacccgc cccacccagc agctccgact gtttctcgtg ctgctccagc
    13261 cagctggcca tttcgctgtt ggcgcgacgg ttagagagca cccatacgga agcgccagtc
    13321 gcactgcggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc gttttgctta
    13381 cgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg ggttaaaacg
    13441 cccttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat ggcgatggtg
    13501 acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc gccgtgtccg
    13561 gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc
    13621 gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt aaacggattg
    13681 cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg tttcgcaacc
    13741 acgggcggcg gaggaggagg tggcggcgca tcggcgttaa tcgtaaagac gacagtgtaa
    13801 ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag atcgaccacc
    13861 agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac cagattattg
    13921 ccgctgaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc gagcgccacg
    13981 gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc
    14041 cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc acccaccggc
    14101 gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat catcccgcca
    14161 cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca
    14221 ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca
    14281 ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa
    14341 taatcgcgaa tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag
    14401 tgataaacca ttaaattgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg
    14461 acattaccct gatgacccag agcttgtaaa aagccccggc taaaggtggt tttacctgcg
    14521 cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg
    14581 cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt
    14641 atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat
    14701 cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgca gcaccgtgcg cgacacagcc
    14761 cgcacaggcg gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa taataccaga
    14821 gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa taatgcctaa
    14881 agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata
    14941 acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca
    15001 gcctaacaac cgtgcggcct cgccaggatg cggcgtgatc acgcgattgt gacgcttatc
    15061 gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt
    15121 ctcaactttt tgcagtgctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac
    15181 caccacatcg gcccattcca ggctttcggt aagagagtcc atcgtcagtt catgcaccat
    15241 caattccggt cgtgcagtca gcagcggcgc aatgttttca ctgcgggtca gtactcggac
    15301 taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc
    15361 gtgatcgcca ccgataatta ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg
    15421 cggttttagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc
    15481 tgccagccaa ctatccagcc ccagtgagtc aaaatgcagt tgtccggtaa catcccgcgc
    15541 ttttccagtg agcaagcctg gtttcagcgc aataaaagtg atggtgtgat cggcgttgat
    15601 cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat caaccgccgc
    15661 aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt cgcggggcgc
    15721 ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt cgggccagac
    15781 aatattcgaa gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc
    15841 ctcttccggc aacggtttgt cgctctcctg ggccagcagc gtgacctcaa tgccgaccgc
    15901 tgtggccagc cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac
    15961 cagccaatgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg cttcgccagc
    16021 gcgaagcatc agctcataga gtgtaagccc cagcgcatct gccgcctcac gttctccgcg
    16081 gcggatatcg tcggcgtgcc agacggtgtg tggtatactt acggggtttt tcttcattgt
    16141 atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg
    16201 ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca gcgagtccga
    16261 gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcacg
    16321 tcacggtatg ctgcgcgctc gcccccatga gttattgccc ggtacgctgc gcgtgatcag
    16381 cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaaccccaa
    16441 actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc tgcgcaaccg
    16501 actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt
    16561 tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc tcggctggac
    16621 aggtaagcac tcacttatcc tcaatcgcga ggccggttct ttcttctttt taggcgaatt
    16681 gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg gtaaatgcgt
    16741 ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg tcgatgctcg
    16801 ccgctgtatc tcttatctca ccatcgaact tgaaggggcg atcccggaag agttgcgacc
    16861 gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg
    16921 ctattcacaa ctcaccacag aagaggattt cagcccgcgt aagccgctac acgcaccgga
    16981 actcattgag ttattcgcct ggagcgaaga gaagttttta aaagtcacgg aaggatcggc
    17041 gattcgtcgt attggtcacc tgcgttggct gcgtaatatc gccgtagcat taggcaatgc
    17101 cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc acccacttct
    17161 cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg cgtgcatagt
    17221 cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg
    17281 tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca attcaagacg
    17341 aataaaatcg tcaagtgatc gactcgacaa aacggaatct taatttttaa cttatctatc
    17401 aataggttaa aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag attacagcct
    17461 gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacgaaaac cagcaataac
    17521 gcatcgcatc gctgtggata ttttattgag agaagaattt gg
//
LOCUS       DAJSKJ010000074        17361 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000074 DAJSKJ010000000
VERSION     DAJSKJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.074"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(246..1412)
                     /gene="ugd"
                     /locus_tag="ON345_002158"
                     /pseudo
     CDS             complement(246..1412)
                     /gene="ugd"
                     /locus_tag="ON345_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(1616..3022)
                     /gene="gndA"
                     /locus_tag="ON345_002159"
     CDS             complement(1616..3022)
                     /gene="gndA"
                     /locus_tag="ON345_002159"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR3072216.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVVA
                     ENPGKKLVPHYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELATT
                     FTEWNEGELSSYLIDITKDIFTKKDEDGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPQAKLAGDKAEFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENASIANLLLAPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
     gene            complement(3285..4178)
                     /gene="galF"
                     /locus_tag="ON345_002160"
     CDS             complement(3285..4178)
                     /gene="galF"
                     /locus_tag="ON345_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR3072217.1"
                     /translation="MVNLKAVIPVAGLGMNMLPATKAIPKEMLPIVDKPMIQYIVDEV
                     VAAGIKEIVLVTHASKNSVENHFDTSYELEALLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQAQPLGLGHSILCAHPIVGDNPFVVVLPDIVLDNASADPLRYNLAAMVARFNETGRS
                     QVLAKRMPGDLSEYSVIKTKEPLDVEGKVSRIVDFIEKPDQPQTLESDLMAVGRYVLS
                     ADIWPELAKTVPGAWGRIQLTDAIAELANKQSVDAMLMTGESYDCGKKMGYMQAFVQY
                     GLRNLKDGAKFRKSIENILVG"
     gene            complement(4313..5710)
                     /gene="wcaM"
                     /locus_tag="ON345_002161"
     CDS             complement(4313..5710)
                     /gene="wcaM"
                     /locus_tag="ON345_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR3072218.1"
                     /translation="MHTLTRRYFLTAGATLAAALPVLSATGARAAEKRSAVDIRDYVH
                     NGDWIRAFNEAFAQADTVNVPASVVCDNINTAITLPPGKTLAVAGALKGNGRGRFILQ
                     KGCQVIGSGDASLYNITLDVRGSDCTIAGLTMSGYGPVAQIFIGGKDKRTLRNLTITR
                     LTITKANYGILRQGYHNQWLDVNITHCHFSYLQGDAIEWNVAINDRNILISDHVIDHI
                     DCTNGKVNWGIGIGLAGSTYDNGYPEELAVKNFVVANITGSNCRQLVHVENGKHFIIR
                     NVKASNITPEFSKQAGIDNATVVIYGCDNFVIDGVDMVNSAGMLIGYGVIKGNYLSIP
                     QNFRLNNIQMDNSALAYTVRGIQISSGNNPSFVSITNLSMKHASLEIHNKPQHIFLRN
                     INVMQLSSRGPALKLNFDLRKDVRGKFMAKQDTLLSLANVRAVNEKGQAAVDIDRVDQ
                     HVVNTERLNFALPRR"
     gene            complement(5720..6943)
                     /gene="wcaL"
                     /locus_tag="ON345_002162"
     CDS             complement(5720..6943)
                     /gene="wcaL"
                     /locus_tag="ON345_002162"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR3072219.1"
                     /translation="MKVSFFLLKFPLSSETFVLNQIVAFIEMGYEVEIVALQKGDLTN
                     THAAYTQYQLADKVRWLQDEPSTALEKLRYRAFSTLRGLHRPAVWRALNARRYGQEAR
                     NLILSSICSRTPAPIVADVYIAHFGPAGVTAAKLRELGVIDGKIATVFHGIDISSRGV
                     LNHYTSEYQQLFRRGDMMLPISQLWADRLQAMGCPTDKITVSRMGVNMDRFSLRPLKA
                     PDGCLQILSVARLTEKKGLHVAIEACRQLKERGVNFRYRILGIGPWERRLRTLIEQYQ
                     LEDHVEMPGFKPSHEVKMLLDEADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVLST
                     VHSGIPELIDSGHSGWLVEEYDATALADKLQSLGRMDAHSLQPVLARAREKVETDFNQ
                     QVINRQLASLLQTLC"
     gene            complement(6940..8220)
                     /gene="wcaK"
                     /locus_tag="ON345_002163"
     CDS             complement(6940..8220)
                     /gene="wcaK"
                     /locus_tag="ON345_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR3072220.1"
                     /translation="MKLLILGNHTCGNRGDSAIMRGLLDAILRQQPDVEMDVMSRFPI
                     SSSWLQGREIIADPLYQLSQKQQAAAGVSGRVKKVLRRRFQHKILLAKVTGEGKLRNF
                     EIASEFHEFVRFLGNYDAIIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMIGHSVGP
                     FQNPDFNELANYVFGRTNALILRETVSRELMAKAQIDTSKVEQGVDTAWLVEHRNDSF
                     EPSYAVQHWLNLTAQRKTVAITLRELEPFDKRLGTTQQAYEKAFAEVVNRMIDDGYQV
                     LALSTCTSIDRYNRDDRMVALRMAQYVKNREHFHIAMDELNDVEIGKILASCELTIGT
                     RLHSAIISMNFGTPAIAINYEHKSAGIMQQLGMSEMAIDIRHLLDGSMQGVVGDILNQ
                     LPALGEKVAAAVEGERQKGFTMIESVLNRIREGR"
     gene            complement(8252..9733)
                     /locus_tag="ON345_002164"
     CDS             complement(8252..9733)
                     /locus_tag="ON345_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="HCR3072221.1"
                     /translation="MGLREKTINGAKWSAMATVVIIGLGLAQMTVLARLFDGHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKAISQIELTTLYWLNVGLGIAVFAAVYLLSDTI
                     ARLLHNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGSIETAAVLSGFTFTV
                     ISACFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLTTVMPNLRFGAWLTA
                     DSIVNYINTNLSTLTLARILGASVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTDKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGDKWLHIVPILQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAVIIGGQLGGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGPGYRDYLLSLWLPFRLSLPTLISSYAVGRLLEGSVSLSALLA
                     MQVLAGVVSFALVIMLSRHSLLLELKKPLCRSGKLKTLLRVPD"
     gene            complement(9743..11137)
                     /gene="wcaJ"
                     /locus_tag="ON345_002165"
     CDS             complement(9743..11137)
                     /gene="wcaJ"
                     /locus_tag="ON345_002165"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR3072222.1"
                     /translation="MTTLKKRERAKTNASLISMVQRFSDITIMLGGLWLVCQAVALPF
                     LYLHLLMALVTLVVFQMIGGITDFYRSWRGVRITTELLLLLQNWTLSLVFAAGLLAFN
                     DDFNTNFTMWLAWYLISTIGMLASRAFIRYGAGMLRHRGYNTRYVAVAGDMPEGQALL
                     ESFRNQPWLGYEVVGSYYDAKPGGVNADWMGNLEQLLDDARSGKIHNVYISMAMQEES
                     QIKEVVRSLADTTCSVLLIPDVFTFNILHSRVEVVNGIPLVPLYDTPLSGVNRIIKRL
                     EDIVLALGILLLISPALCCIALAVKLSSPGPIIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVKQATQNDPRVTRVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSLWLDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(11270..12640)
                     /gene="cpsG"
                     /locus_tag="ON345_002166"
                     /gene_synonym="manB"
     CDS             complement(11270..12640)
                     /gene="cpsG"
                     /locus_tag="ON345_002166"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR3072223.1"
                     /translation="MTKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEYLKPKTIVLG
                     GDVRLTSEALKLALANGLRDAGVDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVNEAARGSYQKINLRDAYIDHLL
                     GYINVQNLTPLKLVVNSGNGAAGPVIDALEARFKELNVPVNLIKVHNTPDGTFPHGIP
                     NPLLPECRADTRDAVIKYGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGARIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRQDDAVYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVSELLCLKGKTLGELVRDRMAAFPASGEINSHLAAPSEAIAR
                     VEAHFAGSALEIDRTDGISLAFADWRFNLRSSNTEPVVRLNVESRGNTPLMQARTQDI
                     LALLNQ"
     gene            complement(12744..14180)
                     /gene="cpsB"
                     /locus_tag="ON345_002167"
     CDS             complement(12744..14180)
                     /gene="cpsB"
                     /locus_tag="ON345_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR3072224.1"
                     /translation="MSQTTLFPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDMTMLQAT
                     VNRLDGVKCASPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRREPDCDPLMLVLAADHVIQDEEAFRAAVRHATPYAENGKLVTFGIVPNVPETGYGY
                     IRRGDVTPGTDDTLAFDVAQFVEKPNLDTAQAYVASGEYYWNSGMFLFRAGRYLEELG
                     KYRPDILRACEKAMDVTDPDLDFIRVDEAAFLSCPDESIDYAVMEQTADAVVVPMDAG
                     WSDVGSWSSLWEISNHTPEGNVHHGDVISHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEKIKADGRHEHHIHREVYRPWGKYDSIDSGDRYQVKRITV
                     KPGEGLSLQMHHHRAEHWIVVAGTAKVTLNDQITLLGENESIYIPLGVTHCLENPGKI
                     PLDLIEVRSGAYLEEDDVVRFQDRYGRV"
     gene            complement(14184..15407)
                     /gene="wcaI"
                     /locus_tag="ON345_002168"
     CDS             complement(14184..15407)
                     /gene="wcaI"
                     /locus_tag="ON345_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR3072225.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWMVQQGHEVRVITAPPYY
                     PEWQVGAKYSSWRYRRENGDATVWRCPLYVPKQPSTLKRLIHLGSFALSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTLLHIQDYEVDAMLGLGMAGNGRAGKVA
                     KLASAFERSGLHNVDNVSTISRSMMNKACEKGVAPEKVIFFPNWSEVSRFREVPETLV
                     MALRQRLGLPDERKIILYSGNIGEKQGLESVITAAEQLREQPWLFAIVGQGGGKARLE
                     KMVSERGLDNVKFLPLQSYEDLPALLAIGDCHLVIQKRGAADAVLPSKLTNILAVGGN
                     AVITAEPQTELGQLCLDNPGIAVCVTPESVPSLVDGIKQALKMPKRNTVARAWAERSL
                     DKEHVLNQFIADIRG"
     gene            complement(15404..15883)
                     /locus_tag="ON345_002169"
     CDS             complement(15404..15883)
                     /locus_tag="ON345_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR3072226.1"
                     /translation="MFLSQEAFATVVQSTPLISIDLIVENAQGEFLLGKRNNRPAQGY
                     WFVPGGRVQKDETLEQAFARLTEAELGLSLPMTAGRFYGVWQHFYDDNFSGTDFSTHY
                     IVLGFRLQVDAGTLTLPDDQHNEYRWLTPPALLASDNVHENSRAYFRVDKLAEVPGL"
     gene            complement(15887..16852)
                     /locus_tag="ON345_002170"
     CDS             complement(15887..16852)
                     /locus_tag="ON345_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR3072227.1"
                     /translation="MTRQRIFVAGHRGMVGSAIVRQLAQRGDVDLVVRSRDELNLLDS
                     REVNDFFAAENIDQVYLAAAKVGGIVANNTFPADFIYENMMIESNIIHAAHTHNVNKL
                     LFLGSSCIYPKMANQPMHESELLQGTLEATNEPYAIAKIAGIKLCESYNRQHNRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALMRRFHEAVQQNAADVVVWGSGTPMREFLHVDDMA
                     AASIHVMELDRGVWLENTQPMLSHINVGTGVDCTIRELAQTMAKVVGFKGRVVFDATK
                     PDGTPRKLLDVSRLHQLGWYHEISLEAGLASTYQWFLENQRHFRG"
     gene            complement(16855..>17361)
                     /locus_tag="ON345_002171"
     CDS             complement(16855..>17361)
                     /locus_tag="ON345_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR3072228.1"
                     /translation="ANIAQGLEKCLYLGNMDSLRDWGHAKDYVKMQWMMLQQDHPEDF
                     VIATGVQYSVRQFVEMAAAQLGIKLRFEGKGVEEKGIVVSVTGHDAPGVKPGDVMVAV
                     DPRYFRPAEVETLLGDPTKAHEKLGWKPETTLQEMVSEMVAKDLEAAKKHSLLKSHGY
                     EVAIALES"
BASE COUNT         3792 a         5106 c         4617 g         3846 t
ORIGIN      
        1 catgccgccc cagcagccca tgacataggc catactcttt taccacttaa gcctcgacca
       61 gtactccttc atcgtacata ccccgtttcc aatttggata tgttcggttt aagctccaca
      121 aatttatagt gggaatgacc tcatcagcgt tggcaaccat tataaaagaa gccctcttga
      181 tgagcgctct atcacatatc accgcccaag caaagcggcg tgaaaaacgt cacagaaact
      241 aacgttcaat cattaccaaa cagatctcgg gtatacacct tctctttcac atcagccaat
      301 tcttccgcca tccggttaga aatgatgaca tccgcttctt gcttaaatgc atccaaatcg
      361 cgtacaacgc gggaattaaa gaattcatct tctttcatcg caggttcata cacaatcact
      421 ggaatgcctt tcgctttgat tcgtttcata atcccttgaa tcgaagatgc gcggaaattg
      481 tcggatcctg acttcatgac gaggcgatat accccaacca ctttaggctt gcgggccagg
      541 atagaatcgg caataaaatc cttgcgggta cggttagcgt caacaatcgc accgacgatg
      601 ttatttggca cagacgcata atttgccagc agttgtttag tatctttcgg gaggcaatat
      661 ccaccataac caaaagaggg attgttatag tgattaccga tgcgcgggtc caggcacaca
      721 ccttcaataa tctggcggga gttcaggccc aggctttccg catagctatc cagctcgttg
      781 aaataagcca cgcgcatcgc gaggtaggtg ttcgcaaata gcttgatagc ttcggcttcg
      841 gtcgagtcgg tgaacagcgt cgggatatcc ttcttcactg ccccttcctg cagcagcgag
      901 gcaaaacgct cggcgcgctc agaacgttcg ccgataacga tacgcgacgg atgcaggttg
      961 tcgtacagcg cacggccttc acgcaggaat tccggcgaga agaaaatatt gtcgataccc
     1021 agtttttctt taatcgaatt ggtaaagcct accggaatgg tagatttgat aaccatcacc
     1081 gcattggggt tgatttcaac gacgtcacgg ataacagact caacgctgga cgtattgaaa
     1141 tagttcgttt tggggtcgta atcggtcggg gtggcgatga tcacatagtc tgcatcgcga
     1201 taagcatcgc gcttatcagt ggtcgcacgg aaattcagcg gtttggtcgc cagatactct
     1261 tcgatctctt tgtcgacgat cggcgactgt ttctagttca gcatgtcgac cttggcctga
     1321 acgatatcca gggccaccac ttcgtggtgc tgggcgatca gaatgccgtt cgacaggccg
     1381 acgtaacctg ttcctgaaat agttatcttc attcgctctg taacttcttc cggttaaaca
     1441 tttgagggtg aatgcatcac caccgtggga cccaacgttg tcggatcggc ttgccgccag
     1501 tatatcgcga attaccgtgt cgccgcctat cagacctgac tgaatgctgt tttcagacaa
     1561 taaaaaagcc cggagatgcg ctccgggcct ggctttacgg atgaactaat tcagattaat
     1621 ccagccattc ggtgtggaac acaccttctt tatcagtgcg tttataggta tgcgcaccga
     1681 agtagtcacg ctgagcctga atcaggttcg ctggcagtac cgcagcgcgg tagctgtcgt
     1741 agtaagccac cgcggcagag aaggtcggta ccggaatacc gttctgcaca gcataggcca
     1801 cgacgtcacg cagcgcctgc tggtattcgt cagcaatatt tttgaagtac ggcgccagca
     1861 acaggttggc gatgctcgcg ttttcagcgt aggcgtcggt gattttctgc aggaactgtg
     1921 cacgaatgat gcagcctgca cggaagatct tcgcgatttc gccgtagttc agatcccagt
     1981 tgtactcgtc agacgcggca cgcagctgag agaagccctg cgcgtaggag acgattttac
     2041 ccaggtacag tgcgcgacgc actttctcaa cgaactctgc tttatcgcca gccagtttag
     2101 cctgcgggcc agtcagcact ttagatgccg ccacgcgctg gtctttcagg gaggagatgt
     2161 agcgcgcgaa aacggattca gtgatcagcg acagcggttc gcccaggtcc agagagctct
     2221 ggctagtcca tttaccggtg cctttgttcg cggcttcgtc gaggatcaca tcaaccaggt
     2281 atttaccatc ttcatctttt ttggtgaaga tgtctttggt gatgtcgatc aggtagctgc
     2341 tcagctcgcc ttcattccac tcggtgaatg tggttgccag ctcttcgtta gacaggttca
     2401 ggccaccttt cagcagagaa taggcttcag caatcagctg catgtcgccg tattcgatac
     2461 cgttgtggac cattttcacg taatgacccg caccgtcagc accgatgtag gttacgcaag
     2521 gctcgccgtc ttcagctaca gcagcaatct tggtcaggat aggcgcaacc aactcatacg
     2581 cttctttctg gccgccaggc atgatagatg ggcctttcag cgcgccctct tcaccaccgg
     2641 acacaccggt accgatgaag ttaaagcctt cagctgacag ctcacggtta cgacgaatgg
     2701 tgtcctggaa gaaggtgtta ccaccatcaa tgatgatgtc acctttatca aggtaaggtt
     2761 tcagggaatc gatggcagca tcggtgcccg cgcctgcttt caccattaac aggatacgac
     2821 gtggggtctc aagagactcg acgaactcct tgaccgtgta gtgaggaacc agctttttgc
     2881 ctggattctc agcgacaact tcttcggttt tttcacggga gcggttgaag atagagacgg
     2941 tataaccacg gctttcgatg ttgagcgcca ggttgcgccc catcacagcc ataccgacta
     3001 cgccgatctg ttgcttggac attacatact cctgtcaggt gtggtcacca cgccacaaat
     3061 gcgcggctta aaatgtgaat tcgatgttaa ctcaggttcg agaaaactgt gtagtcttag
     3121 atgcttttaa atcaacgaaa ctaaaatcgt gctggggttt tagacaagaa accaccacaa
     3181 acgcaacgag aaacaccctg attacttcct aaaaaaaaca gcaatctgac ggagataaat
     3241 aaatcctcat catgattgct gttcttagcc ataatagcga taaattatcc gaccaggata
     3301 ttctctatac ttttacgaaa tttagcccca tccttcaaat tccgcagccc atactgcaca
     3361 aacgcctgca tataccccat cttcttcccg cagtcgtagc tctcgcccgt catcagcatc
     3421 gcgtcgacag actgcttatt cgccaattcg gcgatcgcat ccgtcagttg aattcgaccc
     3481 cacgcccccg gcacggtctt cgccagttca ggccagatat cggcggaaag tacgtagcgg
     3541 cctacggcca tcagatcgga ttccagcgtc tgcggctggt caggtttttc gataaaatca
     3601 acgatgcggc taactttacc ttcaacatcc agcggctctt tcgttttgat gacggagtat
     3661 tcagacaggt cgcccggcat acgtttcgcc agcacctgac tacgcccggt ttcgttgaaa
     3721 cgcgccacca tggcggcaag gttgtagcgc agcgggtcgg cgcttgcgtt gtccagcacg
     3781 atgtccggca acacaaccac aaacgggtta tcgccgacaa tcggatgtgc gcacaggata
     3841 gagtgcccca ggcccagcgg ctgcgcctgg cgaacgttca tgatggtcac gccaggagga
     3901 cagatagatt gaacttcggc cagcagctga cgcttcacgc gctgctcaag cagtgcttcc
     3961 agttcatacg aggtatcgaa gtggttttcc accgagtttt tcgatgcgtg tgtgaccaga
     4021 acgatttctt tgatccctgc agcaaccact tcgtcgacga tgtactgaat catcggcttg
     4081 tcaacgatcg gcagcatctc tttagggatg gccttcgtgg caggtagcat gttcataccc
     4141 aatcccgcta ccggaataac tgctttcaaa ttaaccatta tcttgtccac ctatatatcg
     4201 gttgatgaat tatagcgatt aaacatgttt aatccactct gttttgtcat agtactgatt
     4261 tgagtcagga tgatccctgg atgtaatccg ataattcagt ggattgccgt gctcagcggc
     4321 gcggcagcgc aaaattgagg cgttctgtgt tgacaacatg ctgatcgacc cgatcaatat
     4381 ccaccgccgc ctgccctttc tcattcaccg ccctcacgtt cgccagcgac agcagcgtat
     4441 cctgcttcgc cataaacttc ccacgcacat ccttgcgtaa atcgaagttc agcttcagcg
     4501 caggcccacg agaggagagc tgcatcacgt tgatgttcct caaaaaaatg tgttgcggtt
     4561 tattatgaat ctccagcgag gcgtgcttca tggaaagatt ggtgatagag acaaacgatg
     4621 ggttgttacc cgaggaaatt tgaataccgc ggaccgtata ggccaaagca ctgttgtcca
     4681 tctgtatatt attcaggcgg aagttttgcg gtatcgacaa ataattgcct ttgataacac
     4741 cgtagccaat cagcattccc gcgctattaa ccatatcgac accatcaata acgaaattat
     4801 cacagccgta gatcaccacc gtggcgttat ctattcccgc ctgcttgctg aattccggcg
     4861 tgatattgct ggctttcaca ttacgtataa taaagtgttt gccgttttcc acgtgcacca
     4921 gctggcggca gttgctgccg gtgatgttgg cgacgacaaa gtttttcacc gccagctctt
     4981 ccgggtagcc attatcgtag gtgctgccgg ccaggccgat cccgatgccc cagttgacct
     5041 taccgttggt gcagtcgata tggtcgatga cgtggtcgga gataaggata ttgcggtcgt
     5101 taatcgccac gttccactca atggcgtcgc cctgcagata gctgaagtgg cagtgggtga
     5161 tgttcacatc cagccactga ttatggtagc cctggcgcag aataccgtaa ttggctttgg
     5221 tgatggtcag gcgggtaatg gtcagattac gcagcgtacg cttgtctttc ccgccgatga
     5281 aaatctgcgc caccgggccg tagccgctca tcgtcaatcc ggcaatcgta caatcactgc
     5341 cacgcacgtc cagcgtaatg ttgtaaaggc tggcatcacc gctgccaata acctggcagc
     5401 ccttttgcag gataaagcgc ccgcgcccgt tacctttcag ggcaccggca accgccagcg
     5461 ttttccccgg cggcagcgta atcgccgtat tgatattgtc gcaaaccaca ctcgctggca
     5521 cgttgacggt gtcggcctgc gcgaaggcct cattgaacgc gcggatccag tcgccattgt
     5581 gcacgtagtc acgtatgtcc actgccgagc gcttctcggc ggcacgcgcg ccggttgctg
     5641 aaagtaccgg taaggccgcg gcaagcgttg ccccggcggt aaggaagtaa cgtcgggtta
     5701 aggtgtgcat agcgcctcct tagcaaagcg tctgcagcag gctggcaagc tgccggttaa
     5761 tcacctgctg attaaaatcg gtttcgactt tctcacgtgc gcgcgccagc actggctgca
     5821 ggctgtgcgc atccatacgg ccaagtgatt gcagtttgtc cgccagcgcc gtggcgtcgt
     5881 actcctccac cagccagccg gaatgaccgg agtcaatcag ttccgggatg ccgctgtgga
     5941 ccgtcgacag caccggaatc cccaccgcca tcgcctccat gagcgccacc ggaatacctt
     6001 ccatatcgcc atcggccccc gttaccgagg gcagcaggaa gacgtcagct tcatcaagca
     6061 gcattttcac ttcatgactg ggcttaaaac ccggcatttc tacgtgatct tccaactgat
     6121 actgttcaat cagggtgcgc agacggcgct cccacggccc gatgccgagg atgcgatagc
     6181 gaaagttgac gccacgctct ttcagctgac ggcaggcctc aatcgctaca tgcagcccct
     6241 ttttctcagt cagacgcgca acggagagta tctgcaaaca gccatcgggc gcttttagcg
     6301 ggcgcagcga gaagcggtcc atgtttacgc ccattcttga tacggtaatt ttatccgtcg
     6361 ggcagcccat cgcctgcagc cggtcggccc acagttggct gatgggcaac atcatgtcgc
     6421 cgcggcggaa cagctgctga tattcactgg tgtagtggtt caacactccg cggcttgaga
     6481 tatcaatacc gtgaaagacg gtggctatct tcccgtcgat cacccccagt tcgcgcagct
     6541 tggcggcagt cacgcccgcc gggccaaagt gggcgatgta aacatcggcg acaatcggcg
     6601 cgggcgtgcg gctacagata gaagagagga tcaggttgcg cgcctcctgg ccgtagcgcc
     6661 gcgcgtttaa cgcccgccag acggcgggac ggtgtaaacc gcgcaacgtg ctgaacgcgc
     6721 gatagcgtag cttctccagc gccgttgaag gctcatcctg taaccagcga actttgtccg
     6781 ccagttgata ttgggtataa gccgcatgag tgttggtcag gtcgcccttt tgcagggcga
     6841 caatctccac ctcataaccc atctcgataa acgccacgat ctggtttaag acaaacgtct
     6901 cagacgataa cggaaatttc agcaggaaga agctgacttt catcggccct cccggatgcg
     6961 gttcagcacc gattcaatca tggtgaagcc cttctggcgt tccccctcca ccgccgcggc
     7021 gactttctcg cccagcgccg gaagctggtt gagaatatcg cccaccacgc cttgcatcga
     7081 gccgtcgagc aggtgacgaa tgtcgatggc catctcagac atacccagct gctgcataat
     7141 gcccgctgat ttgtgctcgt agttaatggc aatcgccggc gtgccgaagt tcatcgagat
     7201 aatcgccgag tgcaggcggg taccgatagt cagttcgcag gaggcaagaa ttttgccaat
     7261 ctccacgtca ttaagttcgt ccatggcgat atggaaatgc tcgcggtttt tgacgtactg
     7321 cgccatgcgc agcgcgacca tgcggtcatc gcggttgtag cggtcgatac tggtacaggt
     7381 agaaagcgcc agcacctgat aaccgtcgtc aatcatacgg ttcaccacct cggcaaacgc
     7441 tttttcatag gcctgctggg ttgtccccag acgcttatca aacggctcca gctcgcgcag
     7501 agtaatcgcc acggttttac gctgtgcggt caggttcagc cagtgctgta ccgcgtagct
     7561 cggttcgaaa ctgtcgttac ggtgctcaac cagccaggcg gtgtccacgc cctgctccac
     7621 tttgctggta tcaatttgcg ctttcgccat cagttcacgg cttaccgtct cacgcaaaat
     7681 caacgcgtta gtgcggccaa agacgtagtt cgccagctcg ttgaagtccg ggttctggaa
     7741 cgggccaacg ctgtggccaa tcatgtagat aggcttgttg gccataaagg cgcacagcgg
     7801 gtactcaaag tgagtcaagc cgtagagatc gacgtagttc gacccaccta cctgaataat
     7861 cgcgtcatag ttgccaagga aacgcacaaa ctcatgaaac tctgacgcaa tttcaaagtt
     7921 gcgcagtttg ccttcgccgg tcaccttggc cagcaggatc ttgtgctgga agcggcgacg
     7981 cagcactttc ttcacgcgcc cgctcacccc agccgccgcc tgctgtttct ggctcagctg
     8041 atacagcgga tcggcaataa tctcacgccc ctgcaaccag gaggaactga tcgggaaacg
     8101 gctcattacg tccatttcaa cgtccggctg ctggcgaaga atcgcatcga gcaggccgcg
     8161 cataatcgcg ctatcgccgc ggttgccgca ggtgtggttt cccaaaatta gtaatttcat
     8221 catggcctca tcaaaaaggg gttcaagcaa atcaatcagg aacgcgtaat aacgttttca
     8281 gttttccgct ccgacagagc ggttttttta gttcaagcaa caggctgtgg cgcgacagca
     8341 tgatcaccag cgcaaagctg actacgcccg ccagcacctg catcgccagc agcgcggaga
     8401 gcgaaacgct gccttccagc agtcgcccca ccgcgtagct tgaaatcagc gtcggcaggg
     8461 agagacgaaa cggcagccac aggctcagca ggtaatcgcg gtagcccggc cccagcaccg
     8521 gtttgatcat cacgaaatag ctgaggatgg tgttgataat ttgcaccagc aggaagccca
     8581 gcgtgacgcc aatcgcgccg ccgagctgcc cgccgataat caccgcagga ataaacagga
     8641 aggtcttaaa gacgttgaat ttaaagctga tatccactcg cgctttggcc atcagcagcg
     8701 aaccgatcgg gttgcccacc gagcgcagca gccccaccac gcacagcaac tggaggattg
     8761 gcacgatgtg cagccatttg tcgccgaaca ccagcggcac aaagttattg ctcaccacca
     8821 tcagccccag cagcgccggg aaattgatga tccccaccac cgacagcagc ttgtagaagt
     8881 tcactcgcag cttgtcggtg tcgtcctgaa tcttggcgaa cgccgggaac agcacgcggg
     8941 tgatgattgg gttaagcttc atcggcggca ccaccgccac gttgtaggcg aggttatagc
     9001 ccccggccac gctggcgccg aggatgcgcg cgagcgtcag cgtcgacagg ttagtgttga
     9061 tgtagttaac aatactgtcc gccgtcagcc acgcgccaaa gcgcaggttc ggcatgaccg
     9121 tggtcagcga aaaatgcaga cctggacggt aaatcttgcg gccaaaatag ccgaacagca
     9181 gcgtacgtac ggcgctgttg accagataac ccaaaattgc ggtcatcgcc agcggccaga
     9241 agcaggcgct aatcaccgta aaggtaaagc ccgacagcac cgctgcggtc tcaatggagc
     9301 cgattttgtt aaactccagc tctttttgca tcagcgcacg aaattgctgg ccgtggggga
     9361 tcaccacgaa ggccagcgac agcgttttga tcaacggcgc gagatccggg ttgtgcagca
     9421 accgcgcaat ggtatcgctc agcagataca ccgcggcaaa caccgcaatc cccagcccga
     9481 cgtttagcca gtagagcgtg gtcagctcaa tctggctgat ggctttacgc tgaataatcg
     9541 agttggcgat accaaaatcc gacagcgtgt ccgccagcgc gataatcacc agcgacacgg
     9601 tgagcagacc aaactgatgg ccgtcaaaca gacgcgccag cacggtcatc tgcgccagcc
     9661 ccaagccaat aatcaccacc gtcgccatcg ccgaccattt cgcgccattg atggtttttt
     9721 ctctcaggcc catgtcactt cctcagtagg ctgctttgtt gacaaagccc ttgaacaccg
     9781 tcaggaagac gatcttgata tcgagccaca ggctccattc gcggatgtat tcgagatcga
     9841 actccacccg tttttccatc ttctcgaggg tatcggtttc accgcgccag ccgttgatct
     9901 gcgcccagcc ggtaatgcca ggcttcactt tgtggcgcag catgtagcct tcaatcagtt
     9961 gacggtactg ttcgttgtgc gccaccgcgt gcggacgcgg gccgacgata gacatgccgc
    10021 cggtgagcac gttgataaac tgcggcagtt catccagcga ggtgcggcgc aggaagttgc
    10081 cgacacgggt cacacgcggg tcgttctgcg tcgcctgttt caccaccttg tcgttctcca
    10141 tgaccttcat tgagcggaat ttccacacct tgatcggctt accatccatg ccgtagcggg
    10201 tctggcggaa gatgatgggg cccggcgaac tcagcttcac cgccagcgcg atgcagcaca
    10261 gcgcaggcga aatcagcagc agaatgccca gcgccagcac gatatcctcc agccgtttga
    10321 taatacgatt caccccggag agcggcgtgt cgtacagcgg caccagcgga ataccgttca
    10381 ccacctctac gcgtgagtgc aggatgttga aggtgaaaac atccgggatc agcagcaccg
    10441 agcaggtggt gtcggcgaga gaacggacca cctctttaat ctggctctct tcctgcatcg
    10501 ccatcgagat gtagacgttg tggattttgc cgctgcgggc atcgtcgagc agttgttcaa
    10561 gattgcccat ccagtcggcg ttaacgccgc ccggtttagc atcgtaatag ctgccgacca
    10621 cttcatagcc cagccacggc tggttgcgga agctttcgag cagcgcctgc ccttccggca
    10681 tgtccccggc gacggccaca tagcgggtgt tatagccgcg atggcgcagc atgccagcgc
    10741 cgtagcgaat gaacgcgcgg ctcgccagca tgccgatggt gctaatcagg taccacgcca
    10801 gccacatggt aaagttggta ttaaaatcat cgttaaacgc cagcagcccg gcggcaaaca
    10861 ccaggctcag cgtccagttc tgcaacagca gcagcaattc ggtagtgata cgcacgccgc
    10921 gccaggagcg gtagaaatcg gtaatcccgc caatcatctg gaaaacgacc agcgtcacca
    10981 gcgccatcaa cagatggaga tataaaaacg gcaacgcaac cgcctgacac accagccaca
    11041 atccccccaa catgatggtg atgtcggaaa aacgctgcac catggagatt aacgatgcat
    11101 tcgttttagc tcgttcgcgc ttttttagag tcgtcatcgt tggtcctatt cgtcgtttcc
    11161 cctaaccccg accctctccc caaaggggcg agggagaaaa accaatccgc agcctgcacg
    11221 ttcggttccc tctcccctct ggggagaggg ttagggtgag gggcaataat tactgattaa
    11281 gcaacgccag aatgtcctgc gttcgcgcct gcatcagcgg cgtatttccg cgagactcca
    11341 cgtttaagcg caccaccggt tcggtattgg aagaacgcag gttgaagcgc cagtcggcaa
    11401 acgccaggct gatgccgtca gtacggtcga tctccagcgc gctgccggca aagtgtgctt
    11461 ccacgcgggc aatcgcttct gacggtgccg ccagatggct gttgatttca ccgctcgccg
    11521 ggaacgccgc catgcggtcg cgcaccagtt cacccaacgt tttgcccttc aggcacagca
    11581 gctcgctgac cagcagccag gggatcatgc cgctgtcgca gtaggcaaaa tcacggaagt
    11641 agtggtgggc gctcatttca ccgccgtaaa ccgcgtcgtc ctgacgcatg cgctctttaa
    11701 taaaggcatg accggttttc gacatcaccg gcgtgccgcc cgccgccgtg acgacgtcca
    11761 cggtgttcca cgacagacgc gggtcgtgaa tgatgcgcgc gccggggttc ttttcgagga
    11821 aggcttccgc cagcaggccg acgatgtagt agccctcgat aaactgtcct ttctcgtcga
    11881 acaggaagca gcggtcgaag tcgccatcaa aagcgatacc catatcggca ccgtatttga
    11941 tgaccgcatc gcgggtatcc gcgcggcact ccggcagcag cgggttagga atgccatgcg
    12001 ggaaggtacc gtccggggtg ttgtgcactt tgatcaggtt caccggcaca ttcagctctt
    12061 tgaagcgggc ttccagcgcg tcaatcaccg ggccagccgc gccgttaccg gagttcacca
    12121 ccaacttcag cggcgtcagg ttttgcacgt tgatgtagcc cagcaggtgg tcgatatagg
    12181 cgtcacgcag gttgattttc tggtagctac cgcgcgcggc ctcattgacc ggcgggaagt
    12241 cgttggcttc cgccaggcgc tgtacgtcgc gcaggccggt atcgccgctg atggggcgtg
    12301 cgccttcccg caccagcttc ataccgttgt agtccatcgg gttatggctg gcggtcactt
    12361 cgataccgcc atcaacgccg agatggaagg tggcaaagta aatctcttcg gtgccggaaa
    12421 gcccgatatc gagcacgtca acgcccgcgt cgcgcaggcc attggctaac gccagtttca
    12481 gcgcctcgct ggtcaagcgc acatcgccgc ccagcacgat agttttcggc ttcaggtatt
    12541 caccgtaagc gcggccaatg cgccacgcga tgtcttcatt cagctcttcg ccgagcttgc
    12601 cgcggatgtc gtaggcttta aaacaggtta atttcgtcat gtttttacct ttcttcaggc
    12661 aacagttcgc cctgaccgtt atggccatca attttggtgt cgtgatttgc cggatgcgcc
    12721 gcgcttatcc ggcctgccgg gcgctacacg cgcccgtaac gatcctggaa gcgcaccacg
    12781 tcgtcctctt ccagataggc gccagatcgc acctcaatca ggtcgagcgg gattttcccc
    12841 gggttttcca ggcagtgggt gacgccaagg gggatataga tagattcgtt ctcacccagc
    12901 agcgtaatct ggtcattgag agtgactttg gcggtgcccg ccaccacaat ccagtgttcc
    12961 gcccggtggt gatgcatctg cagcgacagc ccttcccccg gcttcacggt gatgcgctta
    13021 acctgatagc gatcgccgga atcaatcgag tcatacttgc cccacgggcg gtagacttcc
    13081 cggtggatgt ggtgttcgtg gcggccgtcg gccttaatct tctcgaccac ctttttcacg
    13141 tcctgcaccg cgttgcgatc ggcgataagc accgcgtctt tggtttgcac caccaccagg
    13201 tcctttaccc cgacggtcgt caccaggcca gactctgcat agacatagct gttttcggtt
    13261 ttatggctga tgacatcgcc atgatgcacg ttgccttccg gcgtgtggtt gctgatctcc
    13321 cacagcgatg accaggagcc gacatcgctc cagcccgcgt ccatcggcac caccaccgca
    13381 tccgcggtct gctccatcac cgcataatcg atggattcgt ccgggcagct caggaacgcc
    13441 gcttcatcga cgcggataaa gtcgagatcc gggtcggtga cgtccatcgc tttttcgcag
    13501 gcgcgaagaa tatccgggcg atatttgccc agctcttcca gatagcgccc ggcgcggaac
    13561 aggaacatgc cgctgttcca gtaatattca ccgctggcga cataggcctg tgcggtatcg
    13621 agattgggtt tttcgacaaa ctgcgcgacg tcgaaggcca gcgtgtcgtc tgtacccggc
    13681 gtgacatcac cacggcgaat atagccgtag ccggtttccg gcacgttcgg cacgataccg
    13741 aaggtcacca gcttgccatt ttcagcatac ggcgtggcat gacgaaccgc cgcgcggaag
    13801 gcctcttcgt cctgaatcac atggtccgcc gccagcacca gcatcagcgg gtcgcagtcc
    13861 ggttcgcgac gcttagccgc cagcgccgcc agggcgatgg ctggcgccgt gttgcgccct
    13921 gccggttcca gaataatgtt ttcggtcagc ttgttgagct gacgcagctg ttccgccacg
    13981 ataaagcggt gttgctcatt gcagatcacc accgggctgg cgcacttcac gccgtccaac
    14041 cggttcaccg tggcctgcag catggtcatg tcccctttca ggcacaggaa ctgtttaggg
    14101 tacagcactc gggacagcgg ccacagtcgg ctacccgagc caccggccat cacgaccggg
    14161 aaaagcgtag tttgactcat cgtttaaccc cgaatatcag caataaattg gttgagaacg
    14221 tgttctttat cgagcgaacg ttcggcccag gcgcgggcga ccgtatttcg cttcggcatc
    14281 ttcagcgcct gcttaatccc gtcgaccagc gacggaaccg attcaggcgt cacgcacacc
    14341 gcaatgcccg gattgtccag acatagctgg cccagctcgg tctgcggttc agcggtaatc
    14401 accgcgttgc cgcccaccgc cagaatattg gtcagcttgg aaggcaacac cgcatccgcg
    14461 gcgccgcgct tctgaatcac cagatggcag tcgccaatcg ccagcagcgc gggtaaatct
    14521 tcgtacgact gcagcggcag gaacttgacg ttatccagcc cgcgttcgga gaccattttt
    14581 tccagccgcg ctttaccgcc gccctgaccg acaatggcaa acagccacgg ctgttcacgg
    14641 agctgttcgg cggcagtaat cacgctctcc agcccctgtt tctcaccgat attgccggag
    14701 tagagaataa ttttgcgttc atccggcagg ccaagacgct gacgcagcgc catcaccagc
    14761 gtttccggca cctcgcgaaa acgggaaact tccgaccagt tggggaagaa aatcaccttc
    14821 tccggcgcaa cgcctttctc gcaggctttg ttcatcatcg agcgcgagat ggtcgatacg
    14881 ttgtcgacgt tgtgcagccc gctgcgctca aaggcgctgg caagttttgc caccttgccg
    14941 gcacgaccgt tgcctgccat gcccaacccc agcatggcgt cgacttcata atcctgaata
    15001 tgcagcagcg tacgcgcgcc ggagagcttc gccagcaggc gcatccccgg ggtgcagaac
    15061 agcgtcggca ccacgccgat aatgcgatcc ggcttccagc ggcgttgggc catcagcggg
    15121 aagaaactgc tgagcgcaaa gctgccgaga tggatcaaac gcttcagcgt cgacggctgc
    15181 tttggcacat acagcgggca gcgccagacg gtggcatcgc cgttttcccg acggtagcgc
    15241 cagcttgagt atttcgcgcc aacctgccat tccgggtagt acggcggcgc ggtaatcact
    15301 cgcacctcgt gaccctgctg caccatccac tcgaccattt cgccggtgta tttgccgatg
    15361 ccggtgagtt caggcgaata gttgatgcca tacaccagaa tcttcatagg cccggcacct
    15421 cggccaattt gtcgacccgg aaataggcgc ggctgttttc atgaacgttg tccgacgcca
    15481 gtaacgcagg cggtgtcagc caacggtact cattatgctg atcgtccggc agcgttaagg
    15541 tcccggcatc cacctgcagg cggaaaccaa gcacaatgta atgggtcgaa aaatccgtgc
    15601 cggagaagtt gtcgtcatag aaatgctgcc agacgccgta gaagcggcct gccgtcatcg
    15661 gcaggctcag ccccagttcg gcctcggtca ggcgggcaaa cgcctgttcc agcgtttcat
    15721 ctttctgtac ccgaccgccc ggcacaaacc agtacccctg cgccggacga ttgttgcgct
    15781 tacccagcaa aaactcaccc tgcgcgttct caacgatcaa atcgatagag atcagcggtg
    15841 tagactgcac cacggtggca aacgcttcct ggctcagaaa catgcgttac ccccggaagt
    15901 ggcgctggtt ttccagaaac cactggtaag tgctggcaag accagcctcc agcgagattt
    15961 cgtggtacca gcccagttga tgcaggcgag aaacgtcgag cagcttgcgc ggcgtgccgt
    16021 cgggtttggt ggcatcaaac accacccgcc ctttaaagcc caccactttc gccatcgtct
    16081 gcgccagctc acggatggtg cagtcaacgc cggtgccaac gttaatatgc gacagcatag
    16141 gctgcgtgtt ttcgagccag acgccgcgat caagctccat cacatggata ctggctgccg
    16201 ccatatcgtc cacatgcagg aactcgcgca tcggcgtgcc gctcccccac accaccacgt
    16261 ccgcggcgtt ttgctgtacc gcttcatgga atcggcgcat cagcgccgga ataacgtgcg
    16321 aattgctcgg gtggaagtta tcgttcggcc cgtacaagtt ggtcggcatc accgagcgat
    16381 agtcgcgatt atgctggcgg ttgtaggact cgcacagctt gataccggca attttcgcaa
    16441 tcgcgtatgg ttcgttagtc gcttccagcg tgccctgcag cagctcgctt tcgtgcattg
    16501 gctggttagc cattttcgga tagatacacg atgaacccag aaacagcagc ttattcacgt
    16561 tgtgcgtatg cgccgcgtga ataatattgc tttcaatcat catgttctcg tagatgaaat
    16621 ccgccgggaa ggtgttgttg gcgacaatgc cgcccacctt tgccgccgcc agatacacct
    16681 gatcaatatt ttcagcggcg aagaagtcgt tcacttcgcg gctatccagc aggttgagct
    16741 catcgcggct gcgaaccacc agatccacgt cgccgcgttg tgcaagctgg cggacgatgg
    16801 ccgagcccac cattccccgg tggcccgcca caaaaatacg ttgtctggtc atacttaaga
    16861 ctccagcgcg atcgccactt cataaccgtg ggatttcagc agggaatgtt tcttcgctgc
    16921 ttccaggtct ttggcgacca tttcagagac catctcctgc agggtggttt ccggtttcca
    16981 gcccagtttc tcatgcgctt tggtcgggtc gccgagcagg gtttcaactt ctgccggacg
    17041 gaagtagcgc ggatccacgg ccaccatcac atcacccggt ttaacgcctg gcgcatcgtg
    17101 accggtgacg gaaacgacaa tccccttttc ttccacgcct ttaccttcaa agcgcagttt
    17161 gatacccagc tgtgccgccg ccatttcgac gaactggcga accgaatact gcacgccggt
    17221 ggcgataacg aaatcttccg gatggtcctg ttgcagcatc atccactgca ttttcacgta
    17281 gtctttggcg tggccccagt cgcgcaggga atccatgttg ccgaggtaca ggcacttctc
    17341 caggccctga gcgatgttag c
//
LOCUS       DAJSKJ010000075        17253 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000075 DAJSKJ010000000
VERSION     DAJSKJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.075"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            341..1393
                     /gene="ompA"
                     /locus_tag="ON345_002172"
     CDS             341..1393
                     /gene="ompA"
                     /locus_tag="ON345_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR3072229.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(1468..1920)
                     /gene="matP"
                     /locus_tag="ON345_002173"
     CDS             complement(1468..1920)
                     /gene="matP"
                     /locus_tag="ON345_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR3072230.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            2106..3866
                     /locus_tag="ON345_002174"
     CDS             2106..3866
                     /locus_tag="ON345_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR3072231.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            3935..4453
                     /gene="fabA"
                     /locus_tag="ON345_002175"
     CDS             3935..4453
                     /gene="fabA"
                     /locus_tag="ON345_002175"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR3072232.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(4523..4690)
                     /gene="rmf"
                     /locus_tag="ON345_002176"
     CDS             complement(4523..4690)
                     /gene="rmf"
                     /locus_tag="ON345_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR3072233.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(4946..5509)
                     /gene="pqiC"
                     /locus_tag="ON345_002177"
     CDS             complement(4946..5509)
                     /gene="pqiC"
                     /locus_tag="ON345_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR3072234.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(5506..7146)
                     /gene="pqiB"
                     /locus_tag="ON345_002178"
     CDS             complement(5506..7146)
                     /gene="pqiB"
                     /locus_tag="ON345_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR3072235.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(7151..8404)
                     /gene="pqiA"
                     /locus_tag="ON345_002179"
     CDS             complement(7151..8404)
                     /gene="pqiA"
                     /locus_tag="ON345_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR3072236.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(8534..10441)
                     /locus_tag="ON345_002180"
     CDS             complement(8534..10441)
                     /locus_tag="ON345_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3072237.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(10453..12561)
                     /gene="rlmKL"
                     /locus_tag="ON345_002181"
     CDS             complement(10453..12561)
                     /gene="rlmKL"
                     /locus_tag="ON345_002181"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR3072238.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDTIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12805..13914
                     /gene="ycbX"
                     /locus_tag="ON345_002182"
     CDS             12805..13914
                     /gene="ycbX"
                     /locus_tag="ON345_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR3072239.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(13911..14453)
                     /gene="zapC"
                     /locus_tag="ON345_002183"
     CDS             complement(13911..14453)
                     /gene="zapC"
                     /locus_tag="ON345_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR3072240.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDVIKI
                     MNDRLKPQVNVDSFSFEQAV"
     gene            complement(14627..15637)
                     /gene="pyrD"
                     /locus_tag="ON345_002184"
     CDS             complement(14627..15637)
                     /gene="pyrD"
                     /locus_tag="ON345_002184"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR3072241.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(15748..16485)
                     /locus_tag="ON345_002185"
     CDS             complement(15748..16485)
                     /locus_tag="ON345_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR3072242.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGNVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(16451..16966)
                     /locus_tag="ON345_002186"
     CDS             complement(16451..16966)
                     /locus_tag="ON345_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR3072243.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(16974..>17253)
                     /locus_tag="ON345_002187"
     CDS             complement(16974..>17253)
                     /locus_tag="ON345_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3072244.1"
                     /translation="ADVLFSGEADTLAPTLLKLTDTGGSGGMATGIAVQILDAQSQQE
                     IPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGATGGNATAVLYFDLVYQ"
BASE COUNT         4188 a         4596 c         4154 g         4315 t
ORIGIN      
        1 tcctggaact ttttttgtcg cccagccaat gctttcagtc gtgactaatt ttccttgcgg
       61 aggcttgtct gaagcggttt ccgcgatttt cttctgtaaa ttgtcgctga caaaaaagat
      121 taaacatacc ttatacaaga cttttttttc atatgcctga cggagttcac acttgtaagt
      181 tttcaactac gttgtagact ttacatcgct aggggtgctc ggcataagcc gaagatatcg
      241 gtagagttaa tattgagcag atcccccggt gaaggattta accgtgttat ctcgttggag
      301 atattcatgg cgtattttgg atgataacga ggcgcaaaaa atgaaaaaga cagctatcgc
      361 gattgcagtg gcactggctg gtttcgctac cgtagcgcag gccgctccga aagataacac
      421 ctggtacact ggtgctaaac tgggctggtc ccagtaccat gacactggtt ttattcctaa
      481 caatggtccg acccacgaaa accaactggg tgcaggtgct tttggtggtt accaggttaa
      541 cccgtatgtt ggttttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcga
      601 caacatcaac ggtgcataca aagctcaggg cgttcaactg accgctaaac tgggttaccc
      661 aatcactgac gatctggacg tatacactcg tctgggtggt atggtatggc gtgcagacac
      721 caaggctaac gtacctggtg gcgcatcctt taaagaccac gacactggcg tttctccggt
      781 tttcgcaggt ggtgttgagt acgcgatcac tcctgaaatc gctacccgtc tggaatacca
      841 gtggaccaac aacatcggtg acgcaaacac catcggtact cgtccggaca acggtctgct
      901 gagcctgggt gtttcctacc gtttcggtca gggcgaagca gctccggtag ttgctccggc
      961 tccagctccg gcaccggaag tacagaccaa gcacttcact ctgaagtctg acgttctgtt
     1021 caacttcaac aaagcaaccc tgaaaccgga aggtcaggct gctctggatc agctgtacag
     1081 ccagctgagc aacctggatc cgaaagacgg ttccgtagtt gttctgggtt acactgaccg
     1141 catcggttct gacgcttaca accagggtct gtccgagcgc cgtgctcagt ctgttgttga
     1201 ttacctgatc tccaaaggta tcccggcaga caagatctcc gcacgtggta tgggcgaatc
     1261 caacccggtt actggcaaca cctgtgacaa cgtgaaacag cgtgctgcac tgatcgactg
     1321 cctggctccg gatcgtcgcg tagagatcga agttaaaggc atcaaagacg ttgtaactca
     1381 gccgcaggct taagttctcg tctggtagaa aaaccccgca gctgcggggt ttttttcgcc
     1441 tttagtaaat tgaactgact tcgtcagtta ttccttaccc agcaatgcct gcagatcatg
     1501 cttcaaagaa gacattttat tcgcgtattt ctctttgttt tccgcatctt caatcagctg
     1561 aacaatcgtt tcagaaagcg ttttaccgcg acgctgcgca agaccagcca gacgttgcca
     1621 gacaataaat tccagatcga tcgatttttt gcgcgtatgc tggtgttccg cattaaaatg
     1681 gcgcttccgt ctggcccgaa tggtttgctt catgcgattg accagttccg gattcatatg
     1741 cttgtcaatc cagccattta ccagcacggg ttcattttcc agcgagagca acacatcgac
     1801 ggcttcctgg gcggcactgg cttctatgta acgggtgatt aactcccctt cgcgatgctt
     1861 cttcaccaga tacttccatt tccaaccgct ttcaagattt tcaagttgtt gatatttcat
     1921 agcgatctca atgttaccgt gtaactcttt acagaatatc agctttttag gccagtgaag
     1981 aaaagaatct ccatcctgtg agctttaacg cccatgccag gagtattgtc ggtgctttct
     2041 acgtgtgctg tagtgccggt tacggtataa tcgcggcttt gacaacagac taaaaaacat
     2101 caactttgac cattacgaaa cttgcatggc gtgacctggt tcctgatacc gatagctatc
     2161 aggaaatatt tgctcagcca catttgattg acgaaaacga tcctttattc agtgatactc
     2221 aaccgcgact gcaattcgca ctggagcagt tgctgcatac gcgagcatcc tcctctttta
     2281 tgctggcgaa ggccccggaa gagtctgagt atctgaatct tattgccaat gccgcgcgta
     2341 cgctacaaag cgatgcaggc caactggtgg gcggtcacta tgaggtttcc ggccactcca
     2401 tccgcttacg tcacgcagtg agtgcagatg ataattttgc gactttaacg caagttgtcg
     2461 ctgccgactg ggtagaagcg gagcaactct ttggctgcct gcgccagttt aatggcgaca
     2521 ttaccctgca gcctggtctg gtgcatcagg caaatggcgg tattctcatc atctctttgc
     2581 gtacactgct ggcgcaacct ctgctgtgga tgcggctgaa aaatatcgtt aaccgcgagc
     2641 gttttgactg ggttgcgttt gatgagtcgc gccctctccc cgtctctgtg ccttcgatgc
     2701 cattgaagct gaaagtcatt ctggtaggcg aacgcgaatc attggctgat ttccaggaga
     2761 tggagccaga gctttcagag caggctattt atagcgaatt tgaagatact ctgcagattg
     2821 tcgatgcgga gtcagtaacc cagtggtgtc gctgggtgac atttaccgcc agacataatc
     2881 acttacctgc accgggagcg gatgcctggc cggtacttat ccgcgaagca gcacgctaca
     2941 ccggtgaaca agaaacactt ccgcttagcc cgcagtggat cctccgccag tgtaaagagg
     3001 tcgcctccct gtgtgatggc gacaccttct ccggcgagca gctaaactta atgctgcagc
     3061 agcgtgaatg gcgcgaaggt ttcctcgctg aacgtatgca ggatgagatc cttcaggagc
     3121 aaatcctgat tgaaaccgaa ggcgaacgca tcgggcaaat taacgccctt tcggtcattg
     3181 aatttccggg tcatccacgc gcttttggcg aaccttctcg cattagctgc gttgtgcata
     3241 ttggcgatgg tgaattcacc gacatcgaac gcaaagcgga gcttggcggc aatatccatg
     3301 cgaaagggat gatgatcatg caagcgttcc tgatgtcgga actacagctt gagcaacaga
     3361 tccccttctc agcatcgctg acatttgagc agtcatacag tgaagttgat ggagatagtg
     3421 cctcgatggc tgaactctgc gccctgataa gcgccctcgc cgatgtgccg gtgaatcaga
     3481 gtatcgctat cacaggttca gtcgatcagt tcggtcgcgc ccagccggtc ggtggtttaa
     3541 atgagaaaat cgaaggcttc tttgctattt gccagcaacg tgagttaacc gggaaacaag
     3601 gtgtcattat ccccacagct aacgttcgcc atttaagtct tcacagtgaa ctggtgaaag
     3661 cggtagaaga aggcaaattc accatctggg cagtagacga tgtgactgac gcactgccgt
     3721 tattattaaa tctggtgtgg gatggcgaag gccaaacgac gctgatgcaa accatccagg
     3781 aacgtatcgc gcaagcatcg caacaggaag gacgtcaccg ttttccatgg ccattacgtt
     3841 ggctgaactg gtttattccg aactgatcgg acttgttcag cgtacacgtg ttagctatcc
     3901 tgcgtgcttc aataaaataa ggcttacaga gaacatggta gataaacgcg aatcctatac
     3961 aaaagaagac cttcttgcct ctggtcgcgg tgaactgttt ggcgctaaag gcccgcaatt
     4021 gccagcaccg aacatgctga tgatggaccg tgtggtcaaa atgaccgaaa cgggtggtaa
     4081 cttcgacaaa gggtatgttg aagcagaact ggatatcaat ccggatctgt ggttcttcgg
     4141 atgccacttt attggcgatc cggttatgcc gggatgcctg ggcctggacg caatgtggca
     4201 gctggtaggg ttctacctcg gctggctggg cggcgaaggt aaaggccgcg cgctgggcgt
     4261 tggcgaagtg aaattcactg gtcaggtact gccgacagcg aaaaaagtga cctaccgtat
     4321 tcactttaaa cgcattgtta accgtcgtct gattatgggc ctggcggatg gcgaagtgct
     4381 ggttgatggt cgtctgatct ataccgccag cgacctgaaa gtcggtctgt tccaggatac
     4441 gtctgccttc tgattataat cagacagagt atcaaaaggc gaaacctccg caatgcggag
     4501 gtttcttttt aaagagacag aatcaggcca ttactaccct gtccgccatg gcttctcgcc
     4561 agcctcccag ccattgtgac ctttgattca gcgtctgata gggacacatt tcttttgagc
     4621 gtccggcgat gccggcctga taaccacgtt gatgtgcccg ttccaggcga tctcgttttt
     4681 gtctcttcat gcctcgtttc cctcatactg gtttctggtg gaaaagaaaa cagtgattac
     4741 taaatgtgca atcacagtta agcaataccg cgaatcataa ctgccgtcaa tgcgcaaaat
     4801 tcacgccact gtcatatttg tgagctacac gggcatgtcg gttgtacaaa aaatgtgaag
     4861 aggaacaaat ttttccgcac gttagagaca aaaaaaccgc ctcaggcaac ataccggaga
     4921 cggttatatt tacaaatctt tataattaag gtagacgctt tatctcttgt gcaatagaag
     4981 cggcttcctg actccagaca ccggccagca ctttaaccat ctcatcgtaa ccatcctgag
     5041 tttgcactcc ttccagacgg aacggacgtt tgatcagttg tccctggtgg ttcaacagcc
     5101 actcaccact gacaatgacc ttgccatcat agcgaccgtt aaactccgtt acggtaacat
     5161 tgagcgtgtc ctgggcgctt cccagaggct gggaggcaac cacccagccg ggcagttgcg
     5221 tactcaggtt ggcaaccagg gtgttgcgca actgttgatc caacgggctg gcccacaagt
     5281 tgttgttggc aatcacatac ttcacatcac tggtttgata aaccacacca ttccccgcca
     5341 gatagtcagg aacagtgacc tgctctaccc ataacagacg attgccctgg ctggcggtac
     5401 tttgtgtacc gctctgcacc acaggtaact ggtaatagtt tttattaatt tcgccggagc
     5461 tgcatccggc cagccacagt gctgcaatcg tcactagcca ctttttcatt gtttcgccct
     5521 cttcggctct ggatcttttt tgtccttcgc ttcaaatacc agcgcgttac tcttctcatt
     5581 gagcgttttc agcaccggtt gcagttctcg caacacctga tcaaggcgct gcatatccgc
     5641 caccatcttg ttgtaggctg cggagccagg ctggaagccc tgcatgctgc gattcaattc
     5701 acgcaacgtt gactgcatat ccgtcggcaa ctgctgcatc gactggctag cgaggatctt
     5761 gttcatgcta tccagcgtcg tttgcaggtt tttcattgtg cgctgacttt cagaaagcgt
     5821 actggttgcc tgttcaatca tcggattcaa tggcagtttg ttgatcttat ccaacgcttc
     5881 catcagtcgt tgctggattt gcgccaggcc gccgctaacg gtcgggataa tctgataacc
     5941 attaaattca cgaataccgg ttattgcagg cgtatttgga tagaaatcga gatcaacata
     6001 cagtgcacca gtgaccaggt ttccggtttt cagcgatccg cgtaaaccac gtttcaacaa
     6061 ttcgccaagg tgctcaacaa catccgcatt ttcgccaagc tgcattttca gccgctctgg
     6121 ctcgatacga atcagtaccg gaatacggta atcatcgtta aatgtctgac gcatattcgg
     6181 cgcaaagaat ggcactttgc ttacggtacc caggcgaata ccccggaact ctaccggagc
     6241 tcccggttgc agaccgcgta ccgaatcttt aaagaacatc agataatcaa tgtgatcggt
     6301 gtacaacgaa tcctgaatgc tcttctgatc atcatacaaa acgaaagctg ttttcggtgc
     6361 cactggctgc cctaaatcca gaccttccgg cacatcaaag ctgacaccgc cactcagcag
     6421 cgttgtcaat gagcccatct ccacacgcat ccctgctgac gtcagatcaa ccgcaatgcc
     6481 actatctttc cagaagcgaa cattgttggt caccagtcgg tcataaggtg cattgatgaa
     6541 cagttgatag ctgatattgc gtttttgtgt atcgaaggtg ctggtttcaa ccgaacctac
     6601 ccgatagcca cggaacagca ccggatctcc tggcgagagc tgcccggctt ttttgctatc
     6661 gagaatcaca cggatacctt tcgcatcagg cggggccaac ggtggcgagt ccagcaaatc
     6721 gtatttatcc attttgctgc ctttcgcgcc tggctgcagt tcgatataaa ccccagacag
     6781 cagggttccc agaccgctaa tcccttctcg accaatctgc ggtttcacca cccaaaagac
     6841 ggtgtcttta tgcagcaatt tttccatacc ggaattcagc cgcgctttga tttcaacgtg
     6901 cgtcaaatca tcagccagtg tggcgctttc aaccacgccg acgtcaacgc tacggctttt
     6961 aatggtggtt ttgccacctt caattccttc cgcattcgcg gtgatcaggg tcacttccgg
     7021 tccctgatgg ctgtaatgat aaaaaagaac ccaggcccca atgagcgccg tgacgatagg
     7081 aaatatccac acgggagacc agttcttcac tttctggatt ttggcttccc cattattaga
     7141 ttccatactg tcaggactcc tcatgctctg attcaggttg acgatcccac gacaaacgcg
     7201 ggtcaaacgt catagcagaa aacattgtca ttatgacgac taaagcaaac attaatgcac
     7261 ccattgccgg ataaatactc attaaacctc ccatacgcac cagcgccgag agcaccgcga
     7321 taacgaaaac gtcaatcatc gaccagcggc ctacaaactc aacaacttca taaatcaaat
     7381 gcattctttc actgtcgcgc ttgccatgcc ctttggcatc ccaacacagc cacgcgatgg
     7441 cgatcatctt taacgttggc accataatac tggccagaaa gatcaccgca gcgacgggat
     7501 aagagccttc gctccataac agaatgaccc cagcgagaat cgtcgacggc atcttcgagc
     7561 ctaataaatc cgtcaccatg atgggcaaaa tattagccgg aagatacagc atgatggacg
     7621 ttacaagcag cgcgagtgtc cactgcaggc tgtttctacg ccgaacgtac cctttggtac
     7681 tacaacgcgg gcacacgggt tcatcagcag gaaggattgc cgtacaacag gagcacgaac
     7741 gcagcccctg acgtatcccc gtgacgcctg gttttagcgg ctggcgcagt tctggcatcg
     7801 gggcgatgtc gtcccataac cagcgacgat caacgcactg aaaagcgcgc agttgcagga
     7861 cacaaaataa acaccagggg agaaagctgc tgcctacccc aatgctgccg taagccatca
     7921 gtttaacgaa actgaccagc acaccagcga ggaaaatctc cgccattccc caggttttga
     7981 gttgaaaaag cacccgtgcc agttgctctt ttaaacggac cggtaattcc gcgcgattca
     8041 ccagtaacag aatggttatc agacaaaacg cgggaaccag ttgcacaaac aacaggaaaa
     8101 aggtgccgag gctggcgtag tcctcagaaa aaagcacgcc gggaatttcc agtaatgtaa
     8161 tttcactggt aactcctgca acgttcatat tcacaaaagg aaacaagttg gacagcaaca
     8221 gcatgaacag tgcagccaac gcataggcgg tgggacgctg ccgaggggca tcccacgcca
     8281 cggttaacgt tgtgccacac cggggacatg ccgctttctg accatgctca aggcgcggta
     8341 acgccaccag catgtcacac tgcgagcaca ggatgtgctt cgcggcatga tgatgttcgc
     8401 acattggtac tccttaatga tgcgctattt tacagatgac taacaaagtg ttggttttca
     8461 tgcctgataa acccagttca tcaggcaatt ttaccgttgt cagctgtctc tgtttaaatc
     8521 gactattttg cgatcagcca ccatttttta acgcttcaag atactcccag cgttcaaagg
     8581 cttgctccag ctcctgctct gcagcagcca tatcagcaag cactttttgt gtctgctcat
     8641 gtggctgact gaagaaggaa gcatccgcca cctgcgtctg tagggcttcc agcttcgcct
     8701 ccagatcttc gagcaattgc ggtagctgct ccagttcgcg ctgcaattta tagcttagtt
     8761 tgctactgct gcgttttaca gtttctgctt ttgccgcggc ggcttcttcg ctttttttca
     8821 ccgcaggctg tttgagtgcc acatattgtt cttgctgacc acgggcatca tgataaccgc
     8881 caacataacg accaatttta ccgccgcctt cgaagatcca gcattctgta acagtgttat
     8941 cgacaaactg acgatcgtgg ctaaccagca ataccgtgcc ctgatagctg tcgatcagtt
     9001 cttccagcag ttccagcgtt tcgacatcaa gatcgttggt cggttcgtca agaatcaata
     9061 agttgcttgg tttgaggaac aaacgcgcca gcagcaagcg gttccgctca ccgccagaaa
     9121 gcgcacgtac tggcgtcatc gcccgtttcg gatggaacag aaagtcctgc aaatagccca
     9181 atacgtggcg tggcttgcca ttaaccatca cctcttgctt accttcggca aggttatcca
     9241 tcaccgtttt atcgggatcc agttccgcgc ggtgctgatc gaaataagcc acttccagtt
     9301 tggtgccaac gtgaatacgc ccgctgtccg cttgaagctg accgagcatc agtttcagca
     9361 gcgtggtttt gccgcaccca ttcggaccaa tcagggcaat tttgtcgcca cgtagaacct
     9421 gggccgaaaa atctttcacc agttgcttac cgtcaacctg gtagcaaacg tcttccattt
     9481 cgaaaacgat cttaccggag cggctggcct cttccacctg catctttgcg gtacccatca
     9541 cttcgcgacg ttcaccacgc tcgcgacgca ttgctttcag ggcgcgtacg cggccttcat
     9601 tacgggtacg gcgtgctttg atcccctggc gaatccacac ttcttcctgc gccagtttgc
     9661 gatcgaactc ggcattttgt agttcttcca cgcgcagggc ttcttctttt tcctgcaggt
     9721 actggtcgta attccccgga taggtcacca gcttgccgcg atcgagatca acaatgcgcg
     9781 tcgccatatt gcggataaac gaacggtcgt gggagatgaa aataatcgtc ccattgaagg
     9841 ttttaaggaa cccttccagc cagtcgatgg tttcgatatc cagatggtta gtcggttcgt
     9901 ccagcagcaa tacgcgcgga ttgctcacca gcgcgcgtcc taatgccgct ttacgcaacc
     9961 agccgccgga aagcgacgac agcgcaacat taggatctaa ccccagttgc gccagcactt
    10021 cgttgatgcg gttttccagc tgccacaagt tgtggtgatc cagctgttcc tgaaccttcg
    10081 ccagttcgtt gagatttttc tcgctcgggt cgttcatcac caggcgcgaa atatcgtgat
    10141 agcgtttcag atattccgct tgttcttcaa tgccttcggc aacgaaatca taaacgctac
    10201 cctcaacgtt acgcggcgga tcctgttgca gacgagctac aatcaaatct tgctcgtaaa
    10261 taatgcgacc gtcatccagc ccttgttcac ggttgaggat tttcattaac gtcgatttgc
    10321 ctgcgccgtt gcggcctacc agacaaacac gttcattatc ttcgatatgc agttctgcgt
    10381 tatcgagaag cggcgcgtcg ctgaacgaca gccaggcgcc atgcatactg attaatgaca
    10441 ttactattcc tttcaggctg cggtaatcag ccagcagttg tggatctggc ggttacgggc
    10501 gaaatcctgg gagagcgttt tttgcgtaat ttcttgtgct ttcagtccca gttttgccag
    10561 gccgccgaga tccatacgga agccacgttt gttgttcgag aacatgatcg tcccacctgc
    10621 acgcagcaga cgtttcaaat ctttcatcag cgccagatga tcgcgctgaa catcaaacgc
    10681 atcttccatt cgttttgagt tagagaaggt tggcggatcg ataaagatca gatcgaactg
    10741 ttcatttgcc tcacgcagcc acgccaggca atcggcctga atcaggcgat gcgcacgccc
    10801 ggtcaggcca ttcagacgca ggttgcgttc tgcccactcc agataagtac gcgacatatc
    10861 cacggtggtg gtgctgcgtg caccgcctaa tcccgcatgc acggtggcgc tgccggtata
    10921 agagaacagg ttgaggaaat ctttgccttt gctcatctgg ccgagcatac gacgggcgat
    10981 acggtgatcg aggaacagac cggtatcgag gtaatccgtc agattcaccc acaagtgagc
    11041 gttatattcg gtaacttcca gaaactcgcc cttctcgccc agtttctggt actggttttt
    11101 gcccttctgg cgttcacggg ttttcagtac cagtttgttt ggcgcaatcc ccagtaccga
    11161 aatggttgca gcgatagtat cgaacagacg ctgacgcgct ttgtgcgcat caatagtttt
    11221 tggcggcgca tactcctgca ccaccaccca gtcggcataa cggtcaacgg caacgttata
    11281 ttccggcaga tcggcgtcat acaggcggta acattcaatc ccttcctgac gagcccactt
    11341 ctcgaatttt ttgaggttct tacgcaggcg gttggcgtag tcttccgcca ccattgccgg
    11401 tttgctgtct ggagtgcttt cggcaacgtg gtagtttttc tgcacgcagt ccagcgggcc
    11461 gtttttcgcc ttgtactgtt tgtctgcacg cagctgcagg cagctgagca gatccggcga
    11521 ggcgctaaac aaagagagat tccagccacc gaactggttt ttcatgattc ggcccagcag
    11581 actatgcagc gcaatcagcg ccggttcgct gtccagacgt tccccgtatg gcgggttgct
    11641 caacactgta ccgtacggcc ctttcggcag cggattggtc agttgcgcga catctttcac
    11701 ctcaaaggtg atcagttcac caatccccgc aagacgggcg ttagtgcgcg cacgttgaat
    11761 cacccgtgcg tcgctgtccg aaccgtaaaa atgagagcta tactcagcca ggcctttacg
    11821 ggcacgagtt tgcgcttccg ctttcacttc ctgccagata gcttcatcat gctgcgccca
    11881 gccgctaaag ccccaacgcc cacggtgcaa gcctggtgcg cgatcggtcg ccagcatcgc
    11941 cgcttcaatc agcaacgtac cggaaccaca catcggatcg agcagcggtg ttcctggctg
    12001 ccagccggat cgcatcacaa tagcggctgc cagggtttct ttgatcggcg caataccagc
    12061 acgatcgcga tagccacgca gatgtaaacc atcaccactc agatcaagag cgatactggc
    12121 ggtttcttta tgcagccaga cgttaacgcg gatatccggc gcatcgcgat caacatttgg
    12181 acgcggcaga tttttccgcg tgaaagcatc gacgatcgcg tctttcactt tcatcgcgcc
    12241 gtactgactg ttgcggatgg tgtcattcaa accactgaag tggacagcga aggtcgcgcc
    12301 aggattaaac atctctgtcc agttgatcgc ctgaacaccg agatagaggt ctaaatcgct
    12361 gtaaacctta cactcgccca gcggcaacat aatacgcgag gccaggcggc tccacatcag
    12421 gctctggtaa acaagccgtg tgtcgccctt gaaatggacc ccaccctgaa ccacctggca
    12481 ttcaacggcc cccaggtttt ccagttcagt ttttaacagc tcttccagcc cacgggccgt
    12541 actggcaaac agagaattca tatcgtcact tttactctaa gagcctaccg cagtaggcgt
    12601 aattgttgca gtcagtctgg acatggacag cgcggagaaa ccggagcgta cacaagtacg
    12661 tgaggatttc gagcacaacc cagggccaaa ctgacaaata aaatagccta atgggacagg
    12721 cgctaagaaa attgttgcgc attatagcta ataagcgcgt catgtcataa agttgagggc
    12781 ttattttcat ttgaggaccg caccgtggcg acattaaccc ggctttttat tcatcctgtt
    12841 aaatcgatgc gcggcattgg tcttacacat gctctggctg atgtcagtgg tctggccttc
    12901 gatcgcatct ttatgatcac ggaacctgac ggtacgttta ttaccgctcg ccagtttccc
    12961 cagatggtac ggtttactcc ttcacctgtg catgatggct tgcatctcac cgcaccagat
    13021 ggcagtagcg catatgttcg ttttgctgat ttcgccacac aagacgcacc aaccgaagtt
    13081 tggggcacac attttaccgc gcgaattgcg ccagacgcga tcaacaaatg gctaagtgga
    13141 tttttctccc gcgaagtgca attacgctgg gtggggccac aaatgacccg gcgcgtgaaa
    13201 cgccacaaca ctgtacctct gtcatttgct gatggctatc cttaccttct tgctaacgaa
    13261 gcctcgttac gtgatctcca acaacgttgt ccggccagtg taaaaatgga gcaattccgc
    13321 cccaatctgg tggtttccgg cgcgtcagcc tgggaagaag atcgctggaa agtgattcgc
    13381 attggtgatg tggtgtttga tgtggttaaa ccttgtagcc gctgtatttt caccaccgtc
    13441 agcccagaaa aagggcaaaa acatccggca ggcgaaccat taaaaacatt gcaatctttc
    13501 cgcactgccc aggataacgg cgatgtcgat tttggtcaga atttaattgc ccgtaatagc
    13561 ggcgtgattc gcgttggcga tgaggtggaa attctggcaa cggctccggc aaaaatttac
    13621 ggcgcagctg ccgctgatga taccgccaac atcacgcaac aaccggacgc aaatgtagat
    13681 attgactggc agggacaggc atttcgtgga aataaccaac aggtgttgct ggagcaatta
    13741 gaaaatcagg gaattcgtat cccttattct tgccgcgcgg gcatttgtgg aagttgccgt
    13801 gttcagcttt tagaaggcga agtcacgccg ctgaaaaaat cagcaatggg cgatgatggc
    13861 accattcttt gctgtagctg tgtaccgaag actgcactta agttggcgcg ttagactgcc
    13921 tgttcgaagc tgaagctgtc aacattaacc tgcggtttca gcctgtcgtt catgatttta
    13981 atgacgtcgc ccaactgcat cgcgcgaccc gcaataacaa cgcagggttg tgccagcagg
    14041 catagtgcgg cattttcgcc agattcaacg accagcaaat tgacctgctc gtgagtatcg
    14101 ctcaaccaaa cgcaggccgc atcgcccggc atgggcaccc acatttcacc atgggaaaca
    14161 aaatgccagc ttttcggcat ttgcggtttg agatagcgaa ttgctaccag tgcattcagc
    14221 accagttcgg ctttttgttc tttagataaa ttaaaatcgc ggcacttttc ttcaaaagag
    14281 aaatagagcg cggcgtcgtc aacgcaaaag cctgcgggag aaaaggcatc tggagtcaac
    14341 atcttgcgcg caaaacgtga gcgaaatagc ataccattgg ctaaatcgag catcattcga
    14401 tcgtgctctt catcgtaata ccaacgccag ttatcgtctg gtttaattcg catctgcctc
    14461 tcccctaccc tataaaaccg cagcaacaca atccattgtc gcttcgctta ataacctcaa
    14521 caataaaagt acaaaatgtc taaataagca acaacgatga atataaaaca accagggctg
    14581 aaaataaagc cctgattatc ttataaagag gaattcgaag aaataattag atatgggtaa
    14641 cgatttcttt aatcagcggc ggacctttaa aaataaaacc agaataaatt tgcaccagtg
    14701 aggcacccgc agcaatcttt tcacgcgcag cgataaccga gtcgatgccg ccaacaccga
    14761 tgatcggtaa gcgaccgttt aattctagtg acaagcggcg aataatttct gtgcttttta
    14821 actgaagcgg acgaccactt aagccaccgg tttgatcgca atttttcatt ccctgaacca
    14881 gagaacgatc gagagtggta ttggttgcaa taacgccatc aatattatgg cgaactaaac
    14941 tatcggcaac ctggatcaat tcgtcttcag aaagatccgg cgcgatcttc actgcgatcg
    15001 gcacatattt atggtgcatc gcttgcaaat cattttgctt atttttaatc gcggttaaga
    15061 gatcatccag cgcttcacca tattgcagcg tgcgtaatcc tggggtattc ggcgatgaaa
    15121 tattgatggc gatatatccc gcataggcat agattttttc catacaaatc agatagtcat
    15181 ctttgccctg ctccactggc gtatctttat ttttgccgat attaataccc aggacgccgt
    15241 cataatgggc cttttttacg ttctctacga ggttatcaac gccaagatta ttaaagccca
    15301 tacggttgat caaaccttcg gcatctacca gacgaaagag acgcggcttg tcattaccag
    15361 gctgtggacg tggcgtgacg gtaccgatct cgatcgatcc aaatcccatc gcgcctaacg
    15421 catcaatgca ctccccgtct ttatcaagac cggctgccag accaagcgga tttttaaacg
    15481 tcaggcccat gcagttaaca ggtttcgcag gcactttctg ccgcaccagt gcttcaaacg
    15541 gtgttccggt aatgcggcgt aattgctgaa aagtaaactc atgagcgcgc tctggatcga
    15601 gctggaaaag ggctttacga acgaaggggt agtacatgaa ctctcctgga ttcccggtgt
    15661 gcaaaccggg ggcgtattat gtgcgagttc ggaccaaaag ggaattgacc tgcggcaaaa
    15721 aaaacgcaaa cgttttccga acctgtttca gggcagattt tgctccttct ttgcactcaa
    15781 cccgccgtaa tcatttatcg cccgccaggc gacatgacac ggtgctattg cctgacacca
    15841 gcttgtctga cgctggcttt taggtggcac aactcccgca ttacttaacg ctttaccatt
    15901 tacgctcacc tgaatcaggt taatgtagta aggcgttggg ttagtgagtt gtacaccctg
    15961 cgaattccgt gtccagcgta attgttgata agcttccagc gggtcgcccg gcaaaccttc
    16021 gggtcgccag aacagtttaa ataccgagcg catcgccact tttacgctct gattttcaac
    16081 gttgccggat ggcacgctgg caatgcttaa ctcaaatagc gtttcgcgat ccacaggcaa
    16141 gatgtcgctt tccgttctca gcaagcgcaa cgtgcctgta gtaccgggct tcaggagaat
    16201 aagtggcgga gcggccagta atggtgctgg cactgtcgac gcttcccccc cggcccaacg
    16261 cgttgggcgg ttgattttac tgttaataag ccaggattcc tgactggtat tagtcagtaa
    16321 aatagatatc gattctctgt ctgccggaaa gataaatcgc gttccgccga ccaccactcc
    16381 tgcccgcaat tcacccgcca ccagcagcga taaaacggcg aaaatcaaga gcgccaatct
    16441 gttccacgta ttagtcataa tgaagtacaa atgtcgcgct ggcattcact ttacccgact
    16501 gcacatcgct attgacaggt cgcaattgcg cataaaaagt taatggcacc ttaccgctat
    16561 gtaggctgta gtctttactc gcctggttaa gcggaatcaa tatcttcgca tcatccagaa
    16621 tcgcaattgc cagcccgctg gctgcatccg taccactttc aatggacagc atttcgctct
    16681 gcactccgtc gggggttcct gaaaaggtga cgtcaaccgt gctgacatct gttccgcaat
    16741 cctgcaaatt aatgatgaaa gatttctccg ggccataatt cccatgaaaa aaacgtgtca
    16801 gcgagacatc gcctaactgc acattgattt gcttgctatc ctgtgccagc acgcatgtat
    16861 tacggcgaaa cgatccgcta atgttgaccg tgccatcatg ggcaaatagc tcacagcccc
    16921 ccgttaatcc cagtaagaac agaatccaga ttatccgttt caacatcact ccttcactgg
    16981 taaaccagat caaaatagag aaccgccgtc gcattaccgc ccgttgctcc cgcctttgtg
    17041 gacttataac gaagttgata tttgagtgtg ttatccccgg cttttaaggg cgtaagaggc
    17101 tggacctgat tgagcgggat ttcttgctga ctttgcgcat caagaatttg cacggcaatc
    17161 cccgttgcca taccaccgct tccgcctgtg tcagttagtt tgagcagtgt cggcgcaagg
    17221 gtgtcagcct cgccgctaaa aaggacgtcc gcg
//
LOCUS       DAJSKJ010000076        16678 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000076 DAJSKJ010000000
VERSION     DAJSKJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.076"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            260..412
                     /gene="mokC"
                     /locus_tag="ON345_002188"
     CDS             260..412
                     /gene="mokC"
                     /locus_tag="ON345_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR3072245.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(516..1646)
                     /gene="dnaJ"
                     /locus_tag="ON345_002189"
     CDS             complement(516..1646)
                     /gene="dnaJ"
                     /locus_tag="ON345_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3072246.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1735..3651)
                     /gene="dnaK"
                     /locus_tag="ON345_002190"
     CDS             complement(1735..3651)
                     /gene="dnaK"
                     /locus_tag="ON345_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3072247.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4028..4432
                     /locus_tag="ON345_002191"
     CDS             4028..4432
                     /locus_tag="ON345_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR3072248.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTMNSSDMATLKIIGDD"
     gene            4458..5171
                     /gene="msyB"
                     /locus_tag="ON345_002192"
     CDS             4458..5171
                     /gene="msyB"
                     /locus_tag="ON345_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR3072249.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5320..5886
                     /gene="satP"
                     /locus_tag="ON345_002193"
     CDS             5320..5886
                     /gene="satP"
                     /locus_tag="ON345_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR3072250.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5921..6508)
                     /gene="mog"
                     /locus_tag="ON345_002194"
     CDS             complement(5921..6508)
                     /gene="mog"
                     /locus_tag="ON345_002194"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR3072251.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6623..7576)
                     /gene="tal"
                     /locus_tag="ON345_002195"
     CDS             complement(6623..7576)
                     /gene="tal"
                     /locus_tag="ON345_002195"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR3072252.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7855..9285
                     /locus_tag="ON345_002196"
     CDS             7855..9285
                     /locus_tag="ON345_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR3072253.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLEMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLIQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9355..10131
                     /gene="yaaA"
                     /locus_tag="ON345_002197"
     CDS             9355..10131
                     /gene="yaaA"
                     /locus_tag="ON345_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR3072254.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10284..10580)
                     /locus_tag="ON345_002198"
     CDS             complement(10284..10580)
                     /locus_tag="ON345_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR3072255.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10794..12080)
                     /gene="thrC"
                     /locus_tag="ON345_002199"
     CDS             complement(10794..12080)
                     /gene="thrC"
                     /locus_tag="ON345_002199"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR3072256.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12081..13013)
                     /gene="thrB"
                     /locus_tag="ON345_002200"
     CDS             complement(12081..13013)
                     /gene="thrB"
                     /locus_tag="ON345_002200"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR3072257.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13015..15477)
                     /gene="thrA"
                     /locus_tag="ON345_002201"
     CDS             complement(13015..15477)
                     /gene="thrA"
                     /locus_tag="ON345_002201"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR3072258.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15503..15622)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15558..15623)
                     /gene="thrL"
                     /locus_tag="ON345_002202"
     CDS             complement(15558..15623)
                     /gene="thrL"
                     /locus_tag="ON345_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR3072259.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15837..16523)
                     /locus_tag="ON345_002203"
     CDS             complement(15837..16523)
                     /locus_tag="ON345_002203"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR3072260.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3886 a         4426 c         4124 g         4242 t
ORIGIN      
        1 aaagttaaat aattaaatca ttaaaaaatt acgccacaaa aatgcgaggc gtcttcagga
       61 ttagaatcga taaaacaaaa tgggttaaag agagcgatat aatagcggcg ggtgcttgag
      121 gctgtctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag tgatatttta
      181 ccatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta ctgaccgtaa
      241 ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc tgatcgtcat
      301 ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg aggttcacat
      361 ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt aagagcaacg
      421 gcggggagtg atccccgcca ctttacaggt gctcgcatat cttcaacgca ccctattttt
      481 acccaggcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc gtcaaaaaac
      541 ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcgcc ggttgggcca
      601 ccgaagcttt cttgcagctc ttgcagcagc tgtttctgct tctcgttcag gcctaccggt
      661 gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac agacttgacg
      721 cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac tttcagtttg
      781 acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat agcgaagttg
      841 atcgggactt cgcaatacag gttgttgcct tcacgctcga aaatcgggtg ctgtttaacc
      901 tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc accttcgccc
      961 gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga cagcgttttg
     1021 ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttaat cagcgtaccg
     1081 cggccctgac agtgtggaca ggtctgctgc acggcaaaga aaccctgacg catctgcacc
     1141 tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt tgcaccgcta
     1201 ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt cacgccacgt
     1261 acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg cgccgcacgt
     1321 tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat atcgctgaag
     1381 tctgcgccgc cgccaaaacc gccgccgccc atgccgcctt gctcaaacgc agcatgacca
     1441 tactgatcgt atgccgcacg cttttgcgag tcggtcagaa cttcataagc ttccttgatc
     1501 tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg gtatttcatg
     1561 gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga aacgcctaaa
     1621 atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga atgcacgggc
     1681 ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg gcgattattt
     1741 tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg cgttgtttgc
     1801 agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga tttccatcag
     1861 tttctgggaa acctgtgcca gctcctgcat tttcgcttcg atagcggctt tgtcttcacc
     1921 tttcagagca gtttccagtg cagtcagtgc agactcgata gcagttttgt cgtcagccgg
     1981 cagtttgtcg cctgcttctt caacctgctt acgggtgctg tgcagcagat ggtcgccctg
     2041 gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag cttctgcgtc
     2101 gcgtaccatt ttctggattt catcttcgtt cagaccagaa gacgccttga tggtgatctt
     2161 ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aggataccgt cagcatcgat
     2221 atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac catccaggtt
     2281 gaactgaccc agagatttgt tatcagccgc acgtttacgt tcaccctgca gcacatggat
     2341 ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct tggtcgggat
     2401 agtggtgttt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga tacccagaga
     2461 cagcggggta acgtccagca gcagtacgtc tttcacgtca ccagtcagaa caccaccctg
     2521 aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg gctctttacc
     2581 aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac caacgaggat
     2641 aacgtcgtcg atatcagata cggacaggcc agcgtcctgc agtgcaactt tcagcggctc
     2701 aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag tcactttgat
     2761 gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa cgtcggtctg
     2821 ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc gctgcattgc
     2881 cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa ccaggtagtt
     2941 gatcagacgg ctgtcgaagt cttcaccacc taggtgggta tcaccgttgg tagccagaac
     3001 ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat cgaaagtacc
     3061 accacccagg tcataaaccg cgatagtacg gttgccagta cctttgtcca gaccgtaagc
     3121 cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag cgatacggcc
     3181 tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg tgataacagc
     3241 ttcagttacc ggttcaccca agtaatcttc agcggttttc ttcatttttt tcagcacttc
     3301 agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg cgtcgccgtt
     3361 atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt cttcgtcctg
     3421 gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt tcgtcactgc
     3481 ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg caataataga
     3541 aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc catccataat
     3601 cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca tctaaacgtc
     3661 tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga aacgtctggt
     3721 ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact actaaatggg
     3781 gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt gcgcagaaat
     3841 ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata taaagaatga
     3901 atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac gtacctgaat
     3961 cttctcttta tcagcagtaa actagtgggt attcatccct ctacctcttc ccactaagag
     4021 aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc tgatgctgtg
     4081 ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc cgcgtaacga
     4141 aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca tacaactctc
     4201 tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag ccgcccgtag
     4261 cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca ctgactggat
     4321 caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct tttccggtca
     4381 tacgatgaac tcatcggata tggccacgct gaaaattatc ggcgacgact aacgccagaa
     4441 tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg gattttcttc
     4501 agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat aatgaaaaag
     4561 gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg atggaagggc
     4621 atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag cattttggtg
     4681 gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc attttgctcg
     4741 atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt gagattgagc
     4801 aacagttact ggaacaattt ctgcgtaaca cctggaagaa tatggacgag gaacataagc
     4861 aggagtttct gcacgcgatc gatgccaggg tgaatgagct ggaagagctg ctgccgctgc
     4921 tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc caattgaccc
     4981 gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg cgcggcgcgg
     5041 gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc ggcagtgcct
     5101 atcgcgtgac aattccagcc gtactacaaa tcgcctgcct gcgccggatg gttagcgcca
     5161 ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac
     5221 actgccccac tggctgatta ttatgccgcg ccctgaaaac actactgtta ttcagggaaa
     5281 ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac taagttggct
     5341 aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct taacctgcac
     5401 aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt ctacggcggc
     5461 atcgcgcaaa tttttgctgg tctgctggag tacaaaaaag gcaacacttt cggtttaacc
     5521 gcattcacct cttacggttc tttctggctg acgctggttg cgattctgct gatgccgaaa
     5581 ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg tctgtggggc
     5641 gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct gcaattcgtt
     5701 ttctttagcc tgaccgtgct gtttgccctg ctggcgatcg gtaacattgc cggtaacgcc
     5761 gcaatcatcc actttgccgg ctggattggg ctgatctgcg gtgccagcgc aatctatctg
     5821 gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat tggtgaatcc
     5881 cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta acgtcgcgtc
     5941 ttgcactctt cggtctgaat gctgcaacca cttccggtgc cgtttcaacg tatggccctt
     6001 ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca acgttaccct
     6061 cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg ggtaagttaa
     6121 ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca gttggtacaa
     6181 aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg tccgctactg
     6241 ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc gtggtgagca
     6301 ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg atcgcctgct
     6361 catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat gtcagccatt
     6421 cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga tcagagatgg
     6481 aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag tctcgttaaa
     6541 cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag cgtgaccgac
     6601 ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc agtttttcct
     6661 ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt ggatcctggt
     6721 tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc acttcgccgg
     6781 tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc agcagtgccg
     6841 gtgcgatggt cagacggtcg cagcctgcca gttccagaat ttcgccgatg ttacggaagc
     6901 ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag atttcagata
     6961 cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcggta ttcgctttgt
     7021 accagtcaag aatacggcca acaaacggcg agatcaggaa cacgcccgct tccgcacaag
     7081 cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct tctttttcca
     7141 gctgttctgc agcacggata ccctgccagg tagaagccag tttgatcaga atacgatcgt
     7201 tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca attgacgctt
     7261 cagtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga accagtttca
     7321 ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc tgctgcgcgc
     7381 gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta cggtattccg
     7441 gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc ggttgataca
     7501 gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga cgaagggagg
     7561 tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg atgtaaccgg
     7621 tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc
     7681 aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt tttatacaaa
     7741 acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac ttcttaaaca
     7801 attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat atgcatgcca
     7861 gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta cctgctcttc
     7921 ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta catccgccag
     7981 tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac ctcatttcag
     8041 tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg cgttgcgctg
     8101 gctattaccg ccggtggacc tggagccgtc ttttggatgt gggttgccgc gtttatcggc
     8161 atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg tgacgtcaat
     8221 ggacagtttc gtggaggacc ggcatggtat atggcgcgcg ggctggagat gcgttggatg
     8281 ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag cggagttcag
     8341 gcgaacgccg ttgcccgagc cctgagtttt tcttttgatt ttcccccgct ggtgacaggc
     8401 attattctcg ctgtctttgc tctgctggca atcactcgcg gtcttcatgg cgtcgcccgg
     8461 ctcatacagg ggtttgtccc gttgatggcg ataatctggg tgctgaccag catggtgatt
     8521 tgcgtaataa atatcgggca acttccccac gtcatttggt ctatttttga gagtgctttt
     8581 ggctggcagg aagcggcagg cggcgcggcg ggatatacct taagccaggc gattactaac
     8641 ggttttcagc gcagtatgtt ttccaatgag gcgggaatgg gttcgacgcc aaacgcggca
     8701 gcggcagcgg cgtcctggcc tccgcatccg gcagcgcaag ggattgtcca gatgattggc
     8761 atttttatcg acaccctggt catctgtacg gcaagcgcca tgctgatatt actggcgggt
     8821 aacgacacaa cctacatgcc gctggaaggt attcagctta tccagaaggc gatgcgggtg
     8881 ctaatgggtt cctggggtgc tgagtttgtt accctcgtgg ttattctgtt tgccttcagc
     8941 tccatcgttg ccaactacat ttatgccgaa aacaatctct tctttttacg cctgaacaac
     9001 cctaaagcga tctggtgttt gcggatctgc accttcgcaa cggtcatcgg cggcaccttg
     9061 ctaagccttc cgctgatgtg gcaactggca gatatcataa tggcctgcat ggctattacc
     9121 aatttgaccg ccattttact gctctcgcct gtggttcata ccattgccag tgattatcta
     9181 cgccagcgta aactcggcgt gcgcccggtg tttgatccgt tgcgttatcc ggatatcggt
     9241 cgccagcttt ctccggacgc gtgggatgat gtttcgcagg agtaatcaca actatcgatc
     9301 aactcattct ctttttttgc taaagtcggc ataaatttcc tgcaaggact ggatatgctg
     9361 attcttattt cacctgcgaa aacgcttgat taccaaagcc cgttgaccac cacgcgctat
     9421 acgctgccgg agctgttaga caattcccag cagttgatcc atgaagcgcg gaaactgacg
     9481 cctccgcaga ttagcacgct gatgcgcatc agcgacaaac tggcgggtat caacgccgct
     9541 cgctttcatg actggcagcc agatttcacg ccggagaatg cccgccaggc gattctggcg
     9601 tttaaaggtg atgtctacac cggcttgcag gccgaaacct tcagcgaaga cgatttcgat
     9661 tttgcccaac agcatttgcg aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat
     9721 ttaatgcagc cttatcgtct ggaaatgggg atccgtcttg agaatgcccg agggaaagat
     9781 ctgtatcaat tctggggaga tatcatcacc aacaagctga acgaggcgct cgcagcacaa
     9841 ggcgataatg tggtgattaa tctggcgtca gatgagtatt ttaaatcggt gaagccgaag
     9901 aaattgaatg ccgagattat caagccggtg ttcctcgatg agaagaacgg caaatttaag
     9961 ataatcagct tctacgctaa gaaagcacgc ggtctgatga gtcgttttat tattgaaaat
    10021 cggctgacca aaccagagca actgactggt tttaatagcg aaggttactt ctttgatgaa
    10081 gcttcctcca gcaatggcga actggtgttt aaacgctacg agcagcgtta agtttgcagt
    10141 caatgccgga tgcggcgtga acgccttatc cggcctacaa aagcatgcag attcaatata
    10201 ttgcagaatt cacgtaggcc tgataagacg cgccagcgtc gcatcaggcg ctgaatgccg
    10261 gattgttacc cggcatttgt catttagcgg tgatgtttgc ctggaccatg accgccgtga
    10321 tgatcatgag gagctttctt atggtggcgc ggcggtggcg gcggtccgtg tgggtgccag
    10381 cgattgcctc gccattcata atgttgtttc caccagccgt ggtcgcgcca gtgacctcca
    10441 tcccagtaat agccacgatt atcacgatcg cctatctgta attttactga cgggactaac
    10501 gtaatttccg cagcctgtgc tgccatggga gcgaccagaa ccagggaaag tgcgagtacg
    10561 atagattgca tctttttcac aggttactcc ttttatcatt gcccgttatg ggtcgatatc
    10621 cggagattac gcgatgagaa cgacggttgt tattctccgc aatcctaatc tctaagttac
    10681 cgcatttatt gagaatttct cctttttccc tgtcgttttt ctccataatt tcttcataaa
    10741 aaagccgggc tgcataaaag caaacccggc ctgattgaga taatgaatag attttactga
    10801 tgattcatca tcaatttacg caacgcagca aaatcggcgg gcagattatg tgaaagcaag
    10861 ggtaaatcag cacgttctgc cagctctttt ggcagatcca acgtttcacc gagaatcgct
    10921 tccacgctct ctttaaattt cgccggatgc gcggtgccga ggaacaagcc atattcgcct
    10981 ggattcaact ggtcacgcag cgcacgatac gctacggcag cgtgcggctc cgaggtgtag
    11041 cccagttctt ttaactcacg cattgtctgt tgcgtggttt catcatccac ggctgcataa
    11101 cccagctctt tcagttgcca gattttgcgg cggaacaact cttccacacg cggccagttg
    11161 ttcggctgac tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac
    11221 tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc aataaaacgt
    11281 ttcaccggca gaccgagtga cttcgccagc agacccgccg tcaaatcgcc gaagtttccg
    11341 cttggcaccg agacaaccag ctggttgcgc gcttcctgcg gcagctgcgc aactgcttca
    11401 aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc tgagtttaac
    11461 cccagcgcca ctttcagctc ttcatcatca aacgcctgct tcaccagcgc ctgacaggca
    11521 tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc ccaatgtaca gaacagtttt
    11581 tcttgcagtg gactgatttt gcctcgtgga tagaggataa ccactttcac attcggtaaa
    11641 ccgtagaaag catgagccac tgccgctccg gtatcaccgg aggtcgcggt cagaatggtc
    11701 actggcttat cgcccgcaat atgggtcagc atttgtgcca taaagcgacc gccgaaatct
    11761 ttaaatgcca gcgttggccc gtggaacaat tccagacaac cgacatcgct ttcaacattg
    11821 gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat ttcctgcggg
    11881 atttcatcac caataaacgc cgagaggatc ttcgcactgc gggtgacaaa atccagcttc
    11941 agcatctcat caatttcagt caggctgaat tccggcaggt cgtgcggaaa aaacagcccc
    12001 tgatttttgc ccaacccctg ggttacggct tgcgcaaagc tgacctgctc gttgtgatct
    12061 ttcagattgt agagtttcat ttagttttcc agtactcgtg cgcccgccgt atccagccgg
    12121 caaatatgaa caaaaccttc ctgattttgc aggtagttct tacccaacca gtcggcaacg
    12181 cgctgggcgg tatccggctt gtcacataga gcgaacaagg tcgggccgga gccggagata
    12241 ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa gccaggcagt
    12301 aaccgttcac ggtagggttc agcgataaca tctttcatca gcttcgcggc aagctcaggc
    12361 tgacgggaat agcaggcgtg aatgaagcca gccagatgtc gcccgtgcgc aatgcaatcc
    12421 tggcggcgat actgcgccgg taaaatagcc ctggcttctg ccgtcgagac tttaatcccc
    12481 ggatacgcca gcacccacag ccactcatca aaccctggca cttgctggct gatgatgtcg
    12541 ttttcttcga tcatcaactg cataccacca agaaaacacg gtgccacgtt gtcgtaatga
    12601 atgctgccgg agattcgtcc ttccagctcg cccatcaaag ccagcaaacg agtgtcatta
    12661 agcggcttgc cacagtgttc attcatcgcc atcagcgccg cgaccaccga acaggcgctg
    12721 gagcctaagc ccgaaccgat tggcatattc ttttccagag tcatcgccac tggaatttgc
    12781 ttgccaagct cctggcaaaa acgctcccag cactgataaa cgatattttc ccgtggttct
    12841 gacggcagct tatcggcaaa gcgtccgagg ttgttgagac tgaatgtctc tgccgcctca
    12901 accgtgacta catctccgag caatgcacca tcaacaggtg tcaccgccgc cccgagcaca
    12961 tcaaacccga cgctcatatt ggcactggaa gccggggcat aaactttaac catgtcagac
    13021 tcctaacttc catgagaggg tacgtagcag atcggcaaag acaccggcag ctgtaacgtc
    13081 attgcccgca ccatatccgc gcagcaccaa cggcagcggc tgataatagt ggctataaaa
    13141 ggccagggcg ttttcgccat ttttcacttt gaacagcgga tcattaccat ccacttcggc
    13201 aatcttcacg cggcaggcac catcttcatc aatattgcca acatagcgca aaacttttcc
    13261 ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg tcgagctgtg acagattcgc
    13321 cataaaagcg gcaacatcac cctcagcgtt aaactctgcg ggcagcacag gttcaatttc
    13381 aatatccgcc agctccagtt cacgtcccgt ttcacgagcg agaatcaata gcttacgcgc
    13441 tacatccata ccagaaagat catctcgcgg atccggttcg gtataaccca tttcccgcgc
    13501 cagagtagtc gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga tataagaaag
    13561 cgaacctgaa agaatgccgg agaacttcat caattcatca ccagcattga gcagattttg
    13621 caggttctca ataaccggta atccagcccc aacgttggtg tcatagagga atttacgccg
    13681 cgatttttcc gccgcatgac gcaacagatg gtagtaatcc atcgacgagg tgttggcctt
    13741 tttgttcggc gtgacaacgt ggaaaccttc gcgcaagaag tcggcatatt gatccgccac
    13801 tgcctggctg gaggtgcagt caacaatgac cgggttcagc agatgatatt ctttcacgag
    13861 gcgaattaag cgcccgagat taaacggctc tttggcttgc gccagttctt cctgccagtt
    13921 ttccagattt aggccatgca cattggtgag cagtgccttc gagttggcaa caccgcagac
    13981 acgtaagtcg atatgtttat tcttcagcca gctttgttga cgcttcagtt gctccagcag
    14041 cgcaccgcca acgccaccga cgccaatcac aaacacttcg ataacctgat cggtattgaa
    14101 cagcatctga tgagtaacgc gcacgccagt ggtcgcatca tcgttattta ccacgacaga
    14161 gattgagcgt tcagaagatc cctgagcaat ggcgacaatg ttgatattgg cgcgggccag
    14221 cgcggcaaag aatttcgccg agatcccacg caaggtgcgc ataccatcac ctaccaccga
    14281 gataatggcc agccgttccg tcaccgccag cggctccagt aagccttctt tcagttccag
    14341 gtagaactct tcctgcattg cccgttcagc ccgcacacag tcgctttgcg gaacgcagaa
    14401 actgatactg tattcggaag atgattgcgt aatcagcacc acggaaatac gggcgcgtga
    14461 catcgcggca aagacgcgcg ccgccatgcc gaccattcct ttcatccccg ggccggaaac
    14521 gctgaacatt gccatgttat tcagattgga aatgcccttg accggtaatt cgtcttcatc
    14581 acgactggca ccaatgagcg tacctggtgc ttgaggattt ccggtatttt taatcaggca
    14641 agggatctgg aactgggcga tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa
    14701 gtaggaaagc tccatcgctt cctggtagga catcgacttc aacaacctcg catcgggcac
    14761 ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc aacaatcggc
    14821 gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc caagtaccac
    14881 cagttcgcct ttttcattac cggcggtgaa gcctgccatc agcaccatgt gatcagccgg
    14941 aatacgactt gccgcaatac ggcgggtgga ctcagcaata tcgacggtag attcgaggta
    15001 atgccccact gccagtagtt tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc
    15061 ttctaacacg ccggccataa tggcgatcga cattttctcg ccacggcaaa tcagcgcagc
    15121 gttgatgcta tccgggcact gccccaacaa actaatgcca tgcaggacat gttttatttg
    15181 ggcaaattcc tgatcgacga aagttttcaa ttgcgccagc gggaaccccg gctgggcggc
    15241 ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat tgggtaaagc
    15301 atcctggccg ctaatggttt tttcaatcat cgccaccagg tggttggtga ttttggcggg
    15361 ggcagagagg acggtggcca cctgcccctg cctggcattg ctttccagaa tatcggcaac
    15421 acgcagaaaa cgttctgcat ttgccactga tgtaccgccg aacttcaaca ctcgcatggt
    15481 tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc
    15541 tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat ggtggtggta
    15601 atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat ctgtctgtgc
    15661 gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt taatttactc
    15721 acggcaggta accagttcag aagctgctat cagacactct ttttttaatc cacacagaga
    15781 catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg cgttttttat
    15841 ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt gtctcgttgc
    15901 tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc tgccactgcc
    15961 agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg ttggtctgcc
    16021 gttgtgtcac ttttcgtcgg ttgttgtatt aatgttgcta attgatagca atagaccatc
    16081 actgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc agtaagaacg
    16141 tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa caccagcgcg
    16201 gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac tggcgtggcg
    16261 tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc gacatcgtgt
    16321 aacgattcaa ccagtgtcgg gaaaacttta atattatcaa taatatcacc agatccatgt
    16381 gcgacccagc gagtggctgg ctccagatgt gcctgactat cgacaatccg cagttcgcta
    16441 aaccccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct ggcgggtgcg
    16501 accagaataa tcgttatacg catattgcca ctcttcttga ccaaatgacc gcgaacaggg
    16561 tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta agtcgcagaa
    16621 aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact aatggttt
//
LOCUS       DAJSKJ010000077        16473 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000077 DAJSKJ010000000
VERSION     DAJSKJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.077"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(24..176)
                     /locus_tag="ON345_002204"
     CDS             complement(24..176)
                     /locus_tag="ON345_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR3072261.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYELKK"
     gene            complement(441..1175)
                     /gene="cysH"
                     /locus_tag="ON345_002205"
     CDS             complement(441..1175)
                     /gene="cysH"
                     /locus_tag="ON345_002205"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR3072262.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1249..2961)
                     /gene="cysI"
                     /locus_tag="ON345_002206"
     CDS             complement(1249..2961)
                     /gene="cysI"
                     /locus_tag="ON345_002206"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR3072263.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2961..4760)
                     /gene="cysJ"
                     /locus_tag="ON345_002207"
     CDS             complement(2961..4760)
                     /gene="cysJ"
                     /locus_tag="ON345_002207"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR3072264.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5079..5441
                     /gene="queD"
                     /locus_tag="ON345_002208"
     CDS             5079..5441
                     /gene="queD"
                     /locus_tag="ON345_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR3072265.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5519..6790
                     /locus_tag="ON345_002209"
     CDS             5519..6790
                     /locus_tag="ON345_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3072266.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLEDNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDILQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6781..7041
                     /locus_tag="ON345_002210"
     CDS             6781..7041
                     /locus_tag="ON345_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR3072267.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7058..7633
                     /locus_tag="ON345_002211"
     CDS             7058..7633
                     /locus_tag="ON345_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR3072268.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7781..8641)
                     /locus_tag="ON345_002212"
     CDS             complement(7781..8641)
                     /locus_tag="ON345_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR3072269.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8638..9417)
                     /locus_tag="ON345_002213"
     CDS             complement(8638..9417)
                     /locus_tag="ON345_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR3072270.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9395..10804)
                     /locus_tag="ON345_002214"
     CDS             complement(9395..10804)
                     /locus_tag="ON345_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3072271.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10826..12280)
                     /locus_tag="ON345_002215"
     CDS             complement(10826..12280)
                     /locus_tag="ON345_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3072272.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12350..13135)
                     /locus_tag="ON345_002216"
     CDS             complement(12350..13135)
                     /locus_tag="ON345_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3072273.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13454..14731
                     /locus_tag="ON345_002217"
     CDS             13454..14731
                     /locus_tag="ON345_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3072274.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14758..16236
                     /locus_tag="ON345_002218"
     CDS             14758..16236
                     /locus_tag="ON345_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3072275.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFEKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3898 a         4340 c         4161 g         4074 t
ORIGIN      
        1 ggggttttac ccccgcgtgg tggctacttc ttcaattcgt aagcgagaac tgccttaaac
       61 tccataccac gttctctgat actcaactca cacaacgagt cacctaccat cagcgtgaat
      121 cccagtactg tacaacacag tacgatgatt gccacaaggg catattttgt cagcatgtct
      181 tgcctccctg caaagaagag actaacattc gaattgtttg ggttcgaacg taagcctcag
      241 gttgatagaa atatcgcctg gggcttttgt ccatctggaa cctcgcgaat gcttaacgcc
      301 agacagcctc aagcacccaa cgccattcta tacctgataa ttcttcctgc gacgttcacc
      361 tttaaatgcc ggaatgctgc tcgcaaaata aaaacgcccc catcatttct gacagaggcg
      421 tttaatgtat ccggcaatat ttacccttcg tgtaacccac attctctttt taagccaaag
      481 aaacgcgttt cttcttccgc catgccgggc tcccatttac gggttgtatg ggtatcgccc
      541 accgataaat atccttcgtc ccataatggg tgatatttca ggccatgttt ttgcagatac
      601 tgatatatcg ttcggttatc ccagtcgata atcggcagca ctttaaatac gccacgctga
      661 attgccagca ccggtaaatt ggcgcggctg ccggattgtt cgcggcgcag gccagcaaac
      721 cacgtttgcg cattcagttc tttcagcgcc cggttcatcg gttcgacttt gttgatgtca
      781 ttgtactttt caatgccttc aacgccctgt tcccacagtt tgccgtagcg tgcttcctgc
      841 caggcggcgc tttcggtagc gcggtacact ttcaggttga gcttgagttt gtccgttaac
      901 tcgtcaataa agcggtaggt ttccgggaac agatagccgg tgtcggtgag gatcaccgga
      961 atatccgggt gaatctgatt caccagatgc aggctcaccg ccgcctgaat gccaaagctg
     1021 gaagaaagta catattcacc gggcagatta tccagcgccc aggcaacgcg gccttcagcg
     1081 tccagttttt ccagttcggc gttagtttct gccagcgcca gaatgcgatc taccttcggc
     1141 agttcgttca gggcgtttag atcgagtttg gacatagatt cctcactgtt tgccttgcct
     1201 gatgcgacgc tcacgcgtct tatcaggcct acaagaccgg gctgatggtt aatcccacaa
     1261 atcgcgcgcc ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa agtcgccgaa
     1321 gccttcaccc gcttcgcgct ctttcgccca gcgccctatc agttcatcaa gcgacgccag
     1381 gatttccggc tcggtgatgt tttctttata catccgtggg atacgtgtcc caatgcggtt
     1441 gccgccaaga tgcagattgt agcgacccgg tgctttaccc accaggccca cttccgccag
     1501 catcgcgcga ccacaaccgt tcgggcagcc tgttacacgc atcacgatat gctcatcgct
     1561 gacaccatgt ttcgccatta aattatcgat gttgtcgata aaagacggca ggaaacgctc
     1621 cgcttccgcc atcgccagcg ggcaagtcgg gaatgacacg caggccatcg agttttcacg
     1681 ctgcggtgtg acggcattca ttaacccgct ctctttggcg atcttctcga tcttcgcttt
     1741 ctcgctttcc ggcacaccgg cgatgatcag attctggttc gccgtaatgc ggaaatcacc
     1801 tttgtggatc tttgcgatct ccagcaggcc ggttttcagc ggacgccccg gataatcaag
     1861 gatgcgacca ttttcgataa acagcgtcag gtgccagtta tcatcaatgc ccttaaccca
     1921 gccaatacga tctccacgtc cggtgaactc atacggacgg atcggttcaa atttgatccc
     1981 cgcgcgacgc tccacttccg ctttaaacgt ctcaactccc actcgctcca gcgtgtattt
     2041 ggttttggca tttttacgat cggttcggtt accccagtca cgctgagttg tcacgacagc
     2101 ttccgccacc gccagcgtat gctccagcgg cagatagcca aactcgcttg cggtgcgggc
     2161 gtaggttttc ttgttgccgt gttcgataga aagcccaccg cccaccagca agttgaagcc
     2221 caccagcttg ccgttttcgg cgatcgccac gaagttcatg tcgttggcat gcagatcgat
     2281 atcgttctgc ggcgggatca ctaccgtggt tttgaactta cgcggcaggt aggtctgacc
     2341 aaggatcggt tcttcatcag tagtggcgac tttttcctgg tcgagccaga tctccgcata
     2401 cgcgcgggta cgaggcaaca gatgctcaga aatcttcttc gcccactcgt acgcttccgc
     2461 gtgcagctgc gactcgtaag ggttcgaggt gcagagtacg ttacggttca tgtcgttagc
     2521 tgtcgccagc gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt tgacgttctt
     2581 tttcagaatg ccgtggaact ggaatgtctg gcggttggtc aggcgaatgc tgccatagat
     2641 ggtgttttca ccggcaaatt tgtcgatcgc ctgccactgt ttagtggtaa tcaccccacc
     2701 cggcagacga cagcgaagca gcatcgcgtg gcgcggctcc agcttctgtt cagcacgttc
     2761 ggcgcggatg tcgcggtcat cctgctgata catgccgtgg aagcggatca gcaggaagtt
     2821 gtcgccctta aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg tgccgcgcag
     2881 gtagttgctt tcaagcttca tgcgctcggc gtctgtcagt tttccttcga ccactaaagg
     2941 ccctggatgt ttttcgctca ttagtagaca tctcgctgat aacggcgctc tacgcgcagc
     3001 tcacttaaaa attcatccgc cgcttcggtg tccatgccac caaattcggc aatcacttcc
     3061 agaagtgcct gctcaacgtc tttcgccatg cgattagcgt cgccgcagac ataaatgtgg
     3121 gcaccatcat tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt gtcttgtacg
     3181 taaacttttt ctttttggtc gcgcgaccag gcaagatcga tacgtgtcag cacgccatct
     3241 ttgacgtagc gctgccactc cacctggtac aggaagtctt ccgtaaagtg cggattacca
     3301 aagaacagcc agtttttacc tggcgcttcg tcggcggcgc gttgctgcat aaaggcgcgg
     3361 aacggcgcaa taccggtgcc tgggccaatc ataatcaccg gggtttctgg attggctggc
     3421 aggcggaagt tatcgttatg ttcgataaat acgcggactt cgccctcttc ttccacgcgg
     3481 tcagcgagga agctggaggc accaccggca cgggcgcggc cttccacgtc gtaacgcacc
     3541 acaccaacgg tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc gatggaatac
     3601 agacgcggcg tcagcgggcg cagcagatta attagcgctt cggcatcaag ctgtgccggg
     3661 gagaaacgca ccatgtcaac aatcggcgtc gtcgcggcgt aatgctgtaa cttcgcttta
     3721 tcgcccacca gcggcagcag tgtttcgctg cgggtaagcg tggcgtaatt ctcaacaatg
     3781 ttggcggtgt tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag aggcaacgtt
     3841 ttgccctcga cggtgacagg ttcatcgcct ttcagccaca gcagttcgac aagttctttc
     3901 accagtgccg gatcgttctg ataccagacg cccagcgcgt cacccggctg gtaacgcagg
     3961 cccgagtcac ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt acgcccggta
     4021 attttctggt taacagagag gctagccacc agcggcgcgt ctttgctgta cgggctggtg
     4081 tggatttcat ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac aggcgcacgc
     4141 gatttaagcg catcaaccac gcgggcgcgc cactcgctgg cagcagcctg gtattcaaca
     4201 tcggcatcga cacggtcgag caggcgttca ccacccagtt ccgccagctt gctgtcgaaa
     4261 tctttcccgg actggcagaa aaattcataa gagctatcgc cgaggctaaa cacggcaaac
     4321 gcggtatttt ccagctttgg cgcttttttg gagaacagga acttatgcag cgcgacggct
     4381 tcttccggcg gttccccttc cccttgcgtt gacgtcacta cgatgagcag tttttcgctg
     4441 gcgatttgtt tgaatttata gtcgcccgcg ttcaccagct taacgttcag ttttgctgct
     4501 aataaatcat cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg cgaggcggag
     4561 ataatagtta tacccggcat ttctgcggct ggcgctggcg tcgctgcaag cgcagcaggc
     4621 tgctgattga gtacgcccca gaaatagcca gaaacccagg caagctgggt aggcgttaaa
     4681 tcggtcgtgg ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag caacgcggaa
     4741 ggtgggacct gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt ttctgcgccc
     4801 tgtcagcgcc cataaaacag aagagaagat aaggttaacg gggtgaacgg tgtggattaa
     4861 agacgggata gcgataacta ataaccaaat cgactaacct gttttagcaa tagtctttaa
     4921 caacaaaata gattaaccaa cccaatgaaa aacaaatgaa tttagccaat cattaagata
     4981 aatcagcgat tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag cctttccggt
     5041 actctacggc ggttttatcg tcctgtagag aaattatgat gtccaccacg ttatttaaag
     5101 atttcacctt cgaagccgct caccgcttac cacacgtccc ggaagggcat aaatgtggtc
     5161 gcctgcacgg gcattccttt atggtgcgac tggaaattac cggggaagtc gatccgcata
     5221 cgggctggat tatcgatttc gctgaactaa aagcggcgtt taagccaact tatgagcgcc
     5281 tcgatcacca ttatctcaat gatattccag gtctggaaaa cccaactagc gaagttttag
     5341 caaaatggat ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg gtgatggtaa
     5401 aagaaacctg caccgctggt tgtatctatc gcggcgaatg ataagagtgt gtcggcggtc
     5461 aatttccctt aagtaacgct atgttagggt gttgtgttct ggatatctgg ggcatgacat
     5521 ggaagacgac tgcgacatta ttattattgg tgccggtatt gcaggcaccg cttgcgcgtt
     5581 acgctgcgcg cgagcgggtt tatccgtttt gttactggaa cgcgctgaaa tccccggcag
     5641 caaaaatctt tccggcgggc ggttatatac ccatgcactc gcggaactcc tcccgcaatt
     5701 tcatctgacc gcgcctcttg aacgacgcat cactcacgaa agcctttccc tgttaacgcc
     5761 ggatggcgca acgacgtttt ccagcttaca gcccggcggt gaatcctgga gtgtattacg
     5821 tgcacggttc gatccgtggc tggttgccga agccgaagaa gaaggtgtcg aatgcatccc
     5881 cggagcgacg gtggatgcac tgtatgaaga aaacggcagg gtgtgtggtg tcatttgtgg
     5941 tgacgatatt ctccgtgccc gttatgtggt gctggcagaa ggtgccaaca gcgtcctggc
     6001 tgagcgtcac gggttagtga ctcgtcctgc tggtgaagcg atggcgttgg ggatcaaaga
     6061 agtgctgtcg ctggaaacat ccgctattga agaacgtttt catctggagg ataacgaagg
     6121 cgcagcgttg ctgttcagcg gcaggatctg tgatgactta cccggcggcg catttcttta
     6181 tactaatcaa caaacgctct cgttagggat tgtttgcccg ctctcttccc ttacgcaaag
     6241 tcgtgttccg gcaagcgagc tgctgactcg ctttaaagcg catccggcag tgcgcccgct
     6301 tatcaaaaac acggaatcac tggagtatgg tgcgcatctg gtgccagaag gtggcttgca
     6361 cagtatgccg gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg cgttgcgcag
     6421 ttgcgtcaat accggaattt ccgtgcgcgg catggatatg gcgctgactg gcgcgcaggc
     6481 ggcggcacaa acgctgataa gcgcctgcca gcaccgcgag ccgcaaaatc tgtttgcgct
     6541 ttatcatcac aacgtcgagc gcagcctgct gtgggatatt ctacagcgtt atcagcatgt
     6601 tccggcgctt ttgcaacgcc ctggctggta tcgggcgtgg cctgcgttaa tgcaggatat
     6661 ttcccgcgat ttatgggatc agggtgataa acctgttcca ccgctgcgcc agttattctg
     6721 gcgtcattta cgtcgtcatg gcctgtggca tctggcgggc gatgttatca ggagtctgcg
     6781 atgtctgtag cccataatct ctggcgcgtt gctgatgcgc cgcacattgt tccggctgac
     6841 tccgttgagc gtcagatggt acaacggttg attaacgcct gtccggcagg tcttttttcg
     6901 ctcacaccgg aaggtgactt acgcgttgat tatcacggct gcctggagtg tggcacctgc
     6961 cgtttgctgt gcgacgaatc cacactacaa cagtggcgct atccgccttc cggattcggc
     7021 atcacctacc gctttggata aataataagg ataatttatg cccctcttac acctgctccg
     7081 ccagaatccg gtgattgctg ccgttaaaga taatgccagc ctgcaactgg ctatcgattc
     7141 tgaatgccag tttatttccg tgttgtacgg caatatctgc accatcagta atatcgttaa
     7201 aaagattaag aacgccggga aatatgcttt tattcatgtt gatttgctgg aaggcgcgtc
     7261 aaataaagaa gtcgttattc agtttttgaa actggtgacc gaggcggacg gcattatcag
     7321 taccaaagcc tcaatgttga aagcggcaag agcggaaggt tttttctgta ttcatcgcct
     7381 gtttattgtt gattcaattt cgtttcacaa cattgataag caagttgcgc aatcgaatcc
     7441 ggattgtatt gagatcctgc caggctgtat gcccaaagtg ctgggctggg tgacagagaa
     7501 aatccgccaa ccgctgattg ccggtgggct ggtgtgcgat gaagaagatg cgcgtaatgc
     7561 gattaacgcg ggtgtcgtgg cgctttccac cacgaatacc ggggtctgga cgttagcgaa
     7621 aaaattactt tgacgggata atcgtaacca attgaatttg gtttgatttg taggccgcac
     7681 gccacatccg acattcagcg cctgatgcga cgcttgacgc gtcttatcag gcctacaagt
     7741 ccccaggcca gaaccgtagg caggatcatc cggcaacacg ttattggcaa tctgcgtgaa
     7801 tgtttgtcac cagtgcctcc agcaccactt tccagtcatc aaccacgcca acatctgcct
     7861 gtgaaaatac cgcagcgctg gcgtcatgat taatcgccac cacaaacttg ctattgcgta
     7921 cgcccgccat taacgctgct gcgccggaag cgccaacaac aatacacacc tcaggtgcca
     7981 gcagatgtcc ggaaatacca atcacttttt cagcatcaac gccaccgttc atcactcgcg
     8041 cccgactgta gcccacttcg gctcccagct tttctgccag catagcgatt tcctggttat
     8101 ctgcctcacc gccctgcccc accaccagca cccgtctggc ctcagctaat gggtcacggg
     8161 taacattttt cagatcctct gtgctaacca gccagtcggg aagcgcgccg gggacaatgt
     8221 tcagttgctg cataccagag ggcaacgtgg cgtttttcac tgctcccgcc tgacgcgcca
     8281 gggaaaggca tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca tttccccagt
     8341 gggatttgcg cacgcttacg gttggaatat ctaacgaagt cacctggcag atgcttgcac
     8401 catgtaaacg ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc ggcggaaaga
     8461 gaacaacgtc cgcaggcgtt cgctgccact gttccaccaa cgcatccagc acctgttccg
     8521 ccactacagg ctgaggttca atctgccagt gcgccagggt acaaccgctg aaatcctgcg
     8581 ccgccagcca gcttgctatt gcggcattct cctgatttat tgtcacaatt gcgatattca
     8641 tggctgcatc ctttggcgta aatagtcctg ccatagcttt tgagcttttt ctgctaccgt
     8701 ttgcccatca atcagcgtcg ctccgcgtcg ttgctctgct cttgccagtt gcagacactg
     8761 catcgctggc atttcggcag caacagtttt acggataatt tctgctttcc ccgcagccat
     8821 tcgctggcgc attcccggta ctggcaaggc cacttcaccg cactgacgca cggcaatcac
     8881 cgcaggcaag cgaacccggc aacaacgcaa cccatgttca gtacgctgtt cgagggtgat
     8941 aaacagcgcg tcgagagtga aacgctccac ctgggtgaag cagggccagc ccagcatttc
     9001 cgccagcaaa aatggcgttt gcccattctg cccttcgctg ctttggcagc cggtaattat
     9061 cagatccagc ggattctgat gctgccattc ggcaatatgg cgagcgacaa attccggcgc
     9121 aaagcgcaga tctgccgccg tctccagcaa tacagcttcc tcaaacccaa gagccatgag
     9181 atagcgcagc cagtgcaatg cccgttcatc ccccatgctc aacgcggtta aagacatcgg
     9241 cgtaccgttt tttctctgcg ccaacagcag cgcggcagcg gcctgttcat cagcaccgag
     9301 taaacttcgc agtagcgaaa tatccggtcc gcttttaccc tgagccgccg cctgccactc
     9361 tttttccgcc agcattccgg catccggttc ggctttaaac gctaacagaa tgttcattcg
     9421 cacctcctac atttcccgcc gccaccagcg ggagggcttt agtttctggt gcccatagcc
     9481 aggtcacaac aaaaccaacc aacaacactg tcgccaggag caataaggtg acttgcattc
     9541 cccactgcgc cagcacccac ggcagcaggc cagtacttac cgccgcgcca agtcgactca
     9601 tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt tccgcaggca
     9661 aaatgcccac cagattactg actgccgaaa tggtggtgct gaagagaaca aaaagcagca
     9721 gcgttaatga actgccggaa ggcaaacagg ccattactac cagcgttgcc gccagcagca
     9781 aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact aatcccagca
     9841 gcgcgcccac aattaacaac gcattaagca tcaggctggc agtcagcgca tcttccagac
     9901 caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg attacgaggc
     9961 agacaaagaa gacgctgtta aacgccgtgc gccgccagta acgcgaagag aacaaggttt
    10021 tgatgtgttt atgggtcgcc gttaccactt catcgcccag taaaacatgg ggaccaaaat
    10081 agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc cagcgtggtg
    10141 attctggcgt tccccagcgt aataacgtaa tcaacaacgc gggcagagct gccgatgcca
    10201 gtagccagcg ccaggcctcc gggttttcgg aaataaagtg atgtccggca atacttgcca
    10261 gcacatagcc tacggtccac accacgctga atgcgcccag caaaataccg cgatggcggc
    10321 gcggggaaaa ttcagccagc aaggtgtgac ctactgaata atcgcctccc agaccaatgc
    10381 caatcaaaat gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat tgtaaaaacg
    10441 aagcaagcgt aatcagcaaa aagctgaagg tgaagatttt ttgccgacca atatggtcgg
    10501 agatccaccc aagaaccagg ctgccaagga acaaaccaag gagcgccgag ccgccgatca
    10561 ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ctgaataatg gcgtaaccaa
    10621 tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca gcaatgcggc
    10681 agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc atctttaccc
    10741 cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg caacgccccg
    10801 tcatcgcgga acgctgcctg acacattatt tttcaatcgg atagatagta cccgtattca
    10861 taatgccatt aggatcgaac tgctttttca gcccttccag caacgcccac gcgctgccgt
    10921 gttccagttt gctccagtga acgcgatgtt taccgatacc gtggtggtgc accatcgaac
    10981 cgccgaggcg aatggtttct tcacagatga tcttgttgag cggattgtgg tacttgtcga
    11041 tttcctcttc cggcttacag tcaacgacgt tgtaatcgta gacgaagtac atgttggtgc
    11101 cgttctgata gctatgagag gaatgaccgc ccagcatggt gatgtcgtcg gcgtgcggga
    11161 actcagtacg aatacggtta ataacgcttt cgtagatttc gtggatgcag ctccagcagc
    11221 cggacacttc agtggtaaag cccatgttgc cggttttgag gatctgcaca cgttcggcag
    11281 ccactttatc cggtccccag ttcaggttgt taaaccaggt ttcgatcagt ttgctgtcca
    11341 cgcgctggca ctgcgggtag cgggcaacga tttccgcaat cccttcgccc gtcgccttcg
    11401 caatgcgagg attgccttca gccataaaga tcagcacaca tttaccgtcg gcaaaatggg
    11461 tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga cgataccctt
    11521 ccaccatgat ttcacgcagg atgttgaagc cggttttcat gtcttccagg atatagccgt
    11581 agaagaggtt gttttccggg gtgaatttaa agattttcac tgttacttca gtgatatagc
    11641 acaatgcacc ttcgttgccg atgatgatgt gacgaatgtc cgggccagcc gcgcggcgtg
    11701 gcacgttttt aatgcgtgtg acggtgccat ctgccaacac tgcctccaga ccaacgacca
    11761 tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt gctaccaggc
    11821 cgcccatctg cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa cctttttcac
    11881 gcaacgcatt ttccagcact tccaggggaa cgccacattg cgccgtcgcc tgcatattct
    11941 caatatcaat attaatgatt tgattcatgg cggaaccgtc gagcaccacc gagttttcta
    12001 caacagtttc cagcccacct tcggtggcgg aagcaccggt acgcggcaca ccgttaattt
    12061 tgtgcgcatt cataaaattc agcacacggg atacttgctc tgtggaaccg agttttacga
    12121 ccgctgccgg aatcggcaaa gtataaatgc catgaatatc cggaaattta cgaaaacggt
    12181 caatactgtt cttctttaat actgtttcat cggtaattac gcgatctgca ccaacaattt
    12241 cctttagttg gtcgacaatc gctgcgcgag ataaagacat agtaattcct tcctgataat
    12301 aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatggat tagcgcacta
    12361 aataaccacc atcgaccact aataaatgcc cattgacata atttgaagcc ggacttgcga
    12421 ggaatacagc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat
    12481 caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcgtaata tctgttgcat
    12541 aatagccagg ggcaatacca tttacctgaa tattatattg acctagttca tcacaataag
    12601 ctttggtgaa tccggcaaga gcatgtttag tggcagaata tgcaggtgac cattgtccac
    12661 ctaagtaaga gaacaatgaa cagatattaa taattttgcc gcttttctgc gggatcataa
    12721 tttttgccgc ttcatagctt aactcgaatg cggcggtcag gttcacatca atcatcggat
    12781 cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttta
    12841 ccagaatatc aactgtaccg aaacgctcac agcaagcagc gataatcttc tgcggcgcgc
    12901 cttctgcggt gatatccacc tgcatgaagt ccacctcaac accctgtttt tcaatcattt
    12961 cctttgtttc gccgttatct ttgacgaaac taggaataaa gatatttgcg ccagctttgg
    13021 ccaacgccat ggcaaatgcc tggcctaaac cgctattccc accggtaaca attgcggttt
    13081 tacctttcag tgagaaaaaa tccattgaga acgcattgag agattcgatt gacatagtta
    13141 gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag agggaagtta
    13201 cctttctcat tatctctggt aactggagat aatttttagc ataacggcat aactgctaat
    13261 gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag ttttaaaaag cagaagcgtg
    13321 attactctca cacaacacct ctgctttcca tgttatttct tattgcaatt actagagacc
    13381 tgagaaaagt aatccacctg aaagggcggc ttacttttct cttttttttg tctattacaa
    13441 atgggaatac gatatgcaac acaactcata tcgccgttgg ataaccctcg cgataattag
    13501 ttttagcggc ggcgttagtt ttgacctggc ttatttacgt tatatttatc aaattcccat
    13561 ggcgaaattt atgggattca gcaataccga gataggttta ataatgagta cctttggtat
    13621 tgcggctatt attctttatg cccccagcgg cgttattgcc gataaatttt cacaccgcaa
    13681 aatgattact tccgcgatga tcattaccgg attactgggt ctgttaatgg caacgtatcc
    13741 accgctgtgg gtaatgctct gtattcaggt cgcctttgcg ataacgacga ttttaatgct
    13801 gtggtcggtg tcgattaaag ccgcctcgtt gctgggcgat catagtgagc aagggaaaat
    13861 tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg tgtttaccat
    13921 gtgggtcttt tctcgctttg caccggatga cagcgccagc ctgaaaacag tcattatcat
    13981 ctacagtgtg gtttacatct tgttggggat tctgtgctgg ttttttgtta gcgataacaa
    14041 caacctgcgc agtgccaata acgaagaaaa acagtcattc cagcttagcg acatccttgc
    14101 cgttttgcgt atcagcacca cctggtattg cagcatggtg atttttggtg tcttcacaat
    14161 ctacgccatt ctgagttact ccaccaacta tctgaccgaa atgtacggca tgtcgctggt
    14221 ggcggcgagc tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt gcggcccact
    14281 tggcggcatt atcaccacct acagtaaagt gaaatcccct acccgcgtga tccaaatcct
    14341 ttccgtactc ggcctgctgg cgttaactgc cctgctcgtc acgaactcta acccgcaatc
    14401 ggtcgcgatg gggattggcc tgattttact gctgggattc acctgttacg cctcacgcgg
    14461 gctgtactgg gcctgccctg gcgaagcgag aacaccgtct tacattatgg gcaccacggt
    14521 aggtatttgt tcggtgattg gattcctgcc ggatgtcttc gtttacccaa ttatcggcca
    14581 ctggcaagac accctgcccg cagcagaagc ctaccgcaat atgtggctga tgggaatggc
    14641 ggcgcttgcc atggtgattg tctttacctt tttgctgttc caaaaaattc gtactgctga
    14701 tagcgccccc gcaatggcta gcagcaagta agccaggaaa attacaggag aaaaatcatg
    14761 tcgaaaaaat acatcatagg gattgatggc ggaagtcaga gcacaaaagt ggtgatgtac
    14821 gatctggaag gtaatgtggt ttgcgaaggt aaaggcttat tacagccgat gcacacgcct
    14881 gatgccgata ctgcagaaca tcctgacgac gatttatggg catcattatg ttttgcaggt
    14941 cacgatttga tgagccagtt tgctgggaat aaagaagata ttgttggcat cggcctgggc
    15001 tccatccgtt gctgccgtgc gttattgaaa gccgacggca cgccagctgc gccgttgatt
    15061 agctggcagg atgcacgcgt tacacgccct tacgaacata cgaatcctga cgtggcgtat
    15121 gtcacctctt tttcgggtta tctgacgcat cgcttaaccg gcgagtttaa agacaatatc
    15181 gccaactatt ttggtcagtg gccggtggat tataagagct gggcatggag cgaagatgct
    15241 gcggtaatgg ataagtttaa tatcccccgt catatgctgt ttgatgtgca aatgcctggc
    15301 accgtcctcg gacatatcac accacaagcc gcactggcga cacatttccc ggcaggactg
    15361 ccggttgttt gtaccaccag tgataaaccg gtagaagctc tgggggccgg attactggat
    15421 gatgaaactg cggtaatttc tcttggcact tacatcgcat tgatgatgaa cggcaaagca
    15481 ctgccgaaag atccggtggc gtactggccg attatgtctt ctattccgca aacattgctg
    15541 tatgaaggtt acggtattcg caaaggtatg tggacggtga gctggctgcg tgacatgtta
    15601 ggcgagtcgt taattcagga tgccagggcg caggatcttt caccggaaga tttactcaac
    15661 aagaaagcct ctagtgtgcc acctggctgt aatgggctga tgacggtgct ggactggctg
    15721 accaatccgt gggaaccgta caaacgcggg attatgatcg gctttgattc cagcatggat
    15781 tacgcatgga tatatcgttc gatattggaa agcgtggcgc taacgctgaa gaacaattac
    15841 gacaatatgt gtaatgaaat gaatcacttt gagaagcatg tgatcattac tggcggcggt
    15901 tcgaacagcg atctgtttat gcaaattttt gccgacgtgt tcaaccttcc ggcacgccgt
    15961 aacgccatta acggttgtgc aagtctggga gcagcgatta atacagcggt aggtctgggg
    16021 ctatacccgg attacgcaac ggctgttgat aagatggttc gcgtgaaaga tatctttata
    16081 ccgattgaga gcaatgccaa acgctacgac gcgatgaata aaggcatttt caaagaccta
    16141 accaaacata ctgatgtgat cctgaaaaaa tcgtatgaag tgatgcatgg ggaattgggg
    16201 aatgtggatt cgatccagag ctggtcgaat gcgtaagtga ggaaggccgg gcgggaaact
    16261 gcccggcctg aacatacctg aatggttatc cccgctgacg cggggaacat aagttcgaca
    16321 acttaaaaaa tgataacaag tccggtttat ccccgctgat gcagggaaca taataaaaac
    16381 tttaactagt tcggtttatc cctgttttta caaggaatat taactacact cgatgtcttt
    16441 agaataaaag acaatacgaa tcgcacttta tca
//
LOCUS       DAJSKJ010000078        16278 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000078 DAJSKJ010000000
VERSION     DAJSKJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.078"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(225..1226)
                     /locus_tag="ON345_002219"
     CDS             complement(225..1226)
                     /locus_tag="ON345_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3072276.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1333..1629
                     /locus_tag="ON345_002220"
     CDS             1333..1629
                     /locus_tag="ON345_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3072277.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1763..2188)
                     /gene="arsC"
                     /locus_tag="ON345_002221"
     CDS             complement(1763..2188)
                     /gene="arsC"
                     /locus_tag="ON345_002221"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR3072278.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2201..3490)
                     /gene="arsB"
                     /locus_tag="ON345_002222"
     CDS             complement(2201..3490)
                     /gene="arsB"
                     /locus_tag="ON345_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR3072279.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3544..3897)
                     /gene="arsR"
                     /locus_tag="ON345_002223"
     CDS             complement(3544..3897)
                     /gene="arsR"
                     /locus_tag="ON345_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR3072280.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4638..4721
                     /locus_tag="ON345_002224"
     CDS             4638..4721
                     /locus_tag="ON345_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR3072281.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4775..6127)
                     /gene="gorA"
                     /locus_tag="ON345_002225"
     CDS             complement(4775..6127)
                     /gene="gorA"
                     /locus_tag="ON345_002225"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR3072282.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6199..7041)
                     /gene="rlmJ"
                     /locus_tag="ON345_002226"
     CDS             complement(6199..7041)
                     /gene="rlmJ"
                     /locus_tag="ON345_002226"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR3072283.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELESYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7244..9286
                     /gene="prlC"
                     /locus_tag="ON345_002227"
     CDS             7244..9286
                     /gene="prlC"
                     /locus_tag="ON345_002227"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR3072284.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILTLRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9294..10046
                     /gene="rsmJ"
                     /locus_tag="ON345_002228"
     CDS             9294..10046
                     /gene="rsmJ"
                     /locus_tag="ON345_002228"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR3072285.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10095..11564)
                     /gene="dtpB"
                     /locus_tag="ON345_002229"
     CDS             complement(10095..11564)
                     /gene="dtpB"
                     /locus_tag="ON345_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR3072286.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11882..12316)
                     /gene="uspA"
                     /locus_tag="ON345_002230"
     CDS             complement(11882..12316)
                     /gene="uspA"
                     /locus_tag="ON345_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR3072287.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12707..13042
                     /gene="uspB"
                     /locus_tag="ON345_002231"
     CDS             12707..13042
                     /gene="uspB"
                     /locus_tag="ON345_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR3072288.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13286..14785)
                     /gene="pitA"
                     /locus_tag="ON345_002232"
     CDS             complement(13286..14785)
                     /gene="pitA"
                     /locus_tag="ON345_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR3072289.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15017..16219
                     /locus_tag="ON345_002233"
     CDS             15017..16219
                     /locus_tag="ON345_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3072290.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT         4147 a         4136 c         4235 g         3760 t
ORIGIN      
        1 aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca gagaagcatg
       61 attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt ctcaatgcat
      121 tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta cttagaagga
      181 tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa tagcaattga
      241 gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc agctatcatc
      301 ggcattctga acattgattt aagtaatatc acttccgtca ggcttgcgcc ggcactgccg
      361 atgattaatg ccattaatgc tcccagaccc attccttttg tcatcagaac agatgccaga
      421 gggataactg cctcagcccg gatatacagc ggaataccga caacggcgct taatgggatg
      481 gcgaagggat tatctgctcc tgcatgagcg gcaatccacg ctgagggaat aaagccataa
      541 ataaaagatc ctattaatac tcctaacaaa agatatggca gtacatcttt aaactgttgc
      601 aacgcatctt tggtggctag ttttatggga ttaatactta ctacagcttt ggtactgcaa
      661 caggtaacta cggggttctc aatggcttta gccgctggac tacaacaact cacttttatg
      721 ggcgtatatg tcgttcccgg cgaagttttt gctggagcac aacaatttgc tgatgagctt
      781 ttactggcaa tgatatgacg ttcaaacccc agagaatcca ggataatact ggcaagaacg
      841 gagacgccgg ctgcgataat cgcgtacaac aaggtgactt tccaaccaaa ggtcatccac
      901 attagtccga cgataatggg attaagtaat ggtgaaacaa ataaaaaagt gagagtcgga
      961 ccaaacccgg ctttcgctga caacaatcca cgtaacatgg gtattgtcga gcaactacag
     1021 aacggggtaa cggctcccaa cagagcagcc aggagatagc ctctcccttt tctggccccc
     1081 atcatctgct ggattttatg gtctggcacc ttttgtctta tcaggctgac accggcacta
     1141 atcactataa acagcaacga aagctcgacg gcgagaaaca caaacatctc tgcggcatct
     1201 tgcagcatcg caagccaact actcatacgt aacctcattg gataaatcta tatttccaga
     1261 ataatcgaaa tgaaattcag atcaagatat ttctggtatt atcgaaatat caaccagaca
     1321 gaggggggtc atatgcagtt ggaagaagtc gctaaagcgc tgaaagagct aggtcacccg
     1381 acccgtttgt tcatatttaa acatcttgtc aaagccgggg aacaagggtt gccggtcggt
     1441 gagttgcaaa agcagcttgg gatacctggc tccacactca gccaccatat atcagcactg
     1501 gtatcggtag gactggtcaa acaaaaccgt gagagtcgga cgctgatgtg tgtttcgcaa
     1561 tacgcaatgc tggaagcgat aattgaattc ttacgcgagg agtgctgcgt aaatagcaaa
     1621 attgcctgat gcgctacgct ctacatattt catgccatat attgaatttg cacggttttg
     1681 taggtcggat aagacgcgtt agcgtcgcat ccgacataaa caaagcgcac tttaccaact
     1741 atcggcagtg gatatcgccg ctttatttca ggcgcttacc cgcttcatca accactttct
     1801 cgccatcttc cttggagaat gcgccttttt gcgcatctgg cagaatttcc agcaccactt
     1861 ctgaagggcg gcacaggcga gttcccagcg gcgtcaccac aatcgggcga ttaatcagaa
     1921 tcgggtgctg aagcataaag tcgattaacc gatcgtcagt aaatttatct tccgcaaggc
     1981 ccagctcctc atacggttcg acgtttttac gcagcagcgc gcgtacggaa atccccatat
     2041 cggcaatgag tttgaccagt tcatcgcgcg ttggcggagt ttccagataa tggataatgg
     2101 tcggttctgt gccgctgttg cggatcatct ccagcgtatt acgcgacgtg ccgcaggccg
     2161 ggttgtgata aatggtaatg ttgctcatat cagtatctca ttacaaagtg aaagagagac
     2221 gtagcgccag cgcagccagc gtcacaaaca gcacaggcag ggtcatgata atccctgtac
     2281 ggaagtaata tccccagctg atagtcatat tcttctgcga aagtacgtgc agccagagta
     2341 gcgtagccag gctaccaatt ggggtaattt tcggtcccaa atcgcagcca atcacattgg
     2401 cataaaccat cgcttctttg ataacgccag atgccgtgct gccatcaatg gacaacgcgc
     2461 caaccagtac cgtcggcata ttgttcataa tagaagagag gaaggcggtg aggaatccgg
     2521 tgccgagcgt cgcggcccac aggccgttat ccgccagcac gttgagtacg ccagaaagat
     2581 attccgttaa tccggcattg cgcaggccat aaaccaccag atacatgccg agcgagaaga
     2641 tgacaatctg ccagggggca ccgcgcagga ctttacccgt attaatcgca tgaccgcgtt
     2701 tagcgacgac aaataatatc agcgcgccca cagctgcaat ggcgctcacc ggaatgccga
     2761 gcggttccag gacgaaaaat cccaccagca gaagcagtaa aacaacccag ccagttttga
     2821 acgtagcagg atctttgatc gcttctgcgg gagatttcag cagcgccata tcgtagttct
     2881 gcggaatatc tttgcgaaaa tagagatgta acatcaccag cgtggcaaca atcgcggcga
     2941 tatccaccgg caccatcacc gaggcgtatt cgcgaaagcc gaggccaaag aaatcagcgg
     3001 aaacgatatt caccaggttg gagacaataa gcggcaggct ggcggtatcg gcaatgaatc
     3061 cggccgccat cacgaacgcc agcgtagtgc ctttactgaa ccctaaagcc agcagcatgg
     3121 cgatgacaat cggtgtcaaa ataagcgccg cgccatcatt ggcaaacagg gcggcaacgg
     3181 cagcaccgag caggacaatc caggtaaaca gcaagcgacc acgaccatta ccccagcgtg
     3241 agacgtgcag cgccgcccat tcaaaaaagc cggactcatc cagcagcagg ctgatgataa
     3301 tgacggcgat aaacgcagcc gtcgcgttcc agacgatatt ccacaccacc ggaatatcac
     3361 ccggatggac cacgcccgta actaacgcca gtactgcgcc gagcgttgca ctccagccga
     3421 tgcctaaacc tttcggctgc cagataacca atacgatggt caggacaaag atagcgcctg
     3481 ccagtaacat aatgcctccc ggataaaaca catctgaaaa ttcatatgtg tttagctaaa
     3541 tttttaactg caaatgttct tactgtcccc ggaacagttt tgtcgagcca ggttgcggac
     3601 aatcgcctga accttttcct gttcacatcg ccaggcctca tcaataattt tcgccgccca
     3661 tgctggaata tgcggtgata agcggtaatg aacccactta ccttgcttgc ggtccagcaa
     3721 tagcccgctt tcacgcagca atgccaggtg gcgggagatc ttgggctgcg actggtcgag
     3781 agcagtgcag agatcgcaga cgcataactc tcccagttcg ctgagcagta aaacgatgcc
     3841 cagacgggtt tcatcagcaa gaattttgaa caattggatg ggtaacagaa atgacatatt
     3901 gcgctcctga ttgttgcagg tagtgtctct cttcgaagcg gataagtcaa aaacatatat
     3961 gacttaacga atgtgtaagt gcagaggaag gtaataggtg tgaattttga gttggctatt
     4021 catttgaaag gaggtctgaa tcaggacgta aaaaaaccga ctttgcgtcg gttttttact
     4081 ttccagccct gagttggtgg ctctggctgg agtgagagag ttcttatcta acagctcaat
     4141 actattagcc agtggctaac attgcaagaa ttagcgattt cttcagctgg cggagggagc
     4201 acttcggtat atatcgcagt gacgatttat atactttcac tgggtcatcg tcatattaag
     4261 ccttgtccga atccggatgt aaaaaaaccg actttgcgtc ggttttttta ctttccagcc
     4321 ctgagttggt ggctctgact ggagtgagat agccatcatc taacgcatca atgctaatac
     4381 catactgaaa ctattgcaag gacgtgctgg ttttataacc tgcatgtact gtatgattat
     4441 ccagttagct ctgaggcatt ttcactctgg caatgcgcat aaacgctttc aaagtcctgg
     4501 tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagaga gttcttatct
     4561 aacaatgaga catgcgccgt gacaggcagt ggatgagtaa gcggatgcat tctcactcca
     4621 tcgcatggag aaaacgggtg attgataaag caatcatcgt tctaggggcg ttaattgcgc
     4681 tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa cgtaattaag
     4741 ggctaagagc acactactct tagcccttta acatttaacg cattgtcacg aactcttctg
     4801 ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt tttggttgcc cccatcttca
     4861 gcgccaccgc gaagccctgc aacatttcgt ccataccaaa gccaatgccg tgaataccga
     4921 caatcttctc ttccgatcca acgcacacca gcttcatgcg gcacggctgg cggtgagtgg
     4981 tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga tcgtcgccat
     5041 actgctcgcg cgcctgcggt tccgttaaac caacagtacc aatcggcgga tgactgaaga
     5101 ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta aacaggcgtt
     5161 cagagagacg gcgacccgct gcaactgcca ccggtgtcag ctccactgca cccgtgttat
     5221 cgcccaccgc gtaaatacct tcaatgttgg tgttttgata tttatcgacg acgatatagc
     5281 ctttttcgtt ggttttaacg ccagcggctt cgaggttgat gttgtcattg gcaggctcgc
     5341 gaccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc agctccagcg
     5401 tcaggctacc atcggcattt ttcactaccg ctttcgggat ggcgttggtg tgcaactgcg
     5461 ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg aagctgcgca
     5521 gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta ataacgccag
     5581 ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc aaagcaggaa
     5641 gggcaaagaa accatcagaa tcaataccgt attccacgcc cggaatatcc gggtggctcg
     5701 gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg tttacctcca
     5761 gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta tttttaccga
     5821 gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg gcgatcaacg
     5881 tttcccagtt gaatttattg atagtggtat caaaaccata atccgggccg tacatatgga
     5941 tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacacag ccaacattta
     6001 cgcaggtgcc gcccagctct ttggcttcaa tcagcgcaca tttctggccg tacatagccg
     6061 cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt
     6121 tagtcatgac aaagtgtcct tatcgttgat taccgcgatt gtagcgcgag acgtaatagg
     6181 tgccagcaat ggctgcaatt actccggcac gatccagctt acggtggcgt gcccggtgcc
     6241 tgccggaacc agtttgctgt gcagccacgg cagcacgtta ttcatctgtt gttccagttt
     6301 ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat cgctgtctgg
     6361 cagtaccgcc agttcaattt gcagaatttt gcgaataccg gtcgcttcca gatcgtggat
     6421 catgcgctta atttgctgac gcagcaccac cggataccac agtgcgtaag taccagtggc
     6481 gaaacgtttg taaccttctg ctatcccgct gaccaccgct tgatagtcag ttttcatttc
     6541 atacggcggg tcgataagga ttaaaccacg gcgggaaacc ggcggcagtt tggccttaag
     6601 ctgctggaaa ccgtcggctt tttcgacacg cgcacggcta tctttctgaa attcagaacg
     6661 caacaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtcct gttcacgcag
     6721 tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc aactgaccgc tacggttgaa
     6781 gtgttttacc acattgatgt acgactccag ttctgcgggc aaatcgtcct gctgccagat
     6841 acgggcgatg ccttcgagat attcgccggt acgctcggca tgttcgctgc ctaactgata
     6901 acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct ctttcagcga
     6961 ctcgatgatc aggctttgaa cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa
     7021 gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt
     7081 cggggcgcac aaaagcgtat caggacagta taccgaaaag aggccgccgc cgcgaaagcg
     7141 taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt atgtaaagcg
     7201 cctgttgagc gcttccttaa cctctttaac caggactgcg cgaatgacga atccgttact
     7261 gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg ttccagccgt
     7321 gactcaggcg ctgaacgatt gccgcgaaaa tgtggagcgc gtagtagcgc aaggggcacc
     7381 gtacacctgg gaaaatctct gccagccgtt ggcggaagtg gacgatgtgt tggggcgtat
     7441 cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc gtgaagccta
     7501 cgagcaaacc ctgccgctgc tgtcggaata cagcacctgg gtagggcaac atgaaggact
     7561 gtataaagcg tatcgcgacc tgcgcgatgg cgatcattac gccacgctga acacggcgca
     7621 gaagaaagcg gttgataacg cactgcgcga cttcgaactc tctggcatcg gtctgccgaa
     7681 agagaagcag caacgctacg gtgaaattgc cactcgtctt tccgagctgg gcaaccagta
     7741 cagcaacaac gttctcgatg cgacgatggg ctggaccaaa ctcgttaccg acgaagcgga
     7801 gctggcggga atgcctgaaa gtgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga
     7861 acttgaaggc tacctgctga cgctggatat cccaagctac ttgccggtaa tgacctactg
     7921 cgacaaccag gctctgcgcg aagagatgta tcgtgcttac agtactcgcg cctccgatca
     7981 aggcccgaac gccggtaagt gggacaacag caaggtgatg gaagagatcc tcaccctgcg
     8041 ccacgaactg gcgcaactgc tgggctttga aaactacgcc tttaaatccc ttgccactaa
     8101 aatggcagaa aacccgcagc aagtgctgga tttcttaacc gatctggcaa aacgcgcgcg
     8161 tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc gctaaagccg aatttggcgt
     8221 ggatgagttg cagccgtggg atatcgcgta ctacagcgaa aaacaaaaac agcacctcta
     8281 cagcatcagt gacgaacagc tgcgtccgta cttcccggaa aacaaagcgg ttaacggttt
     8341 gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag atgttgatgt
     8401 ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa aataacgaac tgcgcggcag
     8461 cttctacctc gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga tggatgactg
     8521 cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa ccggtcgcgt atttgacttg
     8581 taacttcaac cgcccggtaa atggtaaacc ggcgctgttc actcacgacg aagtgatcac
     8641 cctgttccac gagttcggtc acggcctgca ccatatgctg acccgcatcg aaaccgctgg
     8701 tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga
     8761 aaactggtgc tgggagccgg aggcgctggc gtttatctct ggtcactatg aaaccggcga
     8821 accgctgccg aaagagttgc tggataaaat gctggcggcg aagaactacc aggcggcgct
     8881 gtttattctg cgtcagctgg agttcggcct gtttgatttc cgccttcatg ccgagttccg
     8941 cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa atcaagaaac tggttgccgt
     9001 ggtgccatct ccgtcctggg gccgtttccc gcacgctttc agccatattt tcgccggtgg
     9061 ttatgcggca ggttactaca gctacctgtg ggctgacgta ctggcggcag atgctttctc
     9121 gcgctttgag gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat
     9181 tctgagccgt ggcggttcag aagagccgat ggatctgttc aaacgcttcc gtggtcgtga
     9241 accgcagctg gatgcgatgc tggagcatta cggcattaag ggctgatcat tcagtgaaaa
     9301 tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg gcggcccgct
     9361 gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacgccg gaacatctgg
     9421 aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt gttggcggag
     9481 cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg
     9541 gcattaaagg cgattatttg ccggatgtgg tggatgccac tgcaggactg gggcgcgatg
     9601 cctttgtgct ggcttcagtc ggttgccgcg tgcggatgct ggagcgtaat ccagttgttg
     9661 ccgcgctgct cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt
     9721 tgcaggagcg tttgcagtta attcacgcct ccagcctgac ggcgctaacc gatattaccc
     9781 cacgcccgca ggtggtctat ctcgacccga tgttcccgca taagcagaaa agcgcgctgg
     9841 tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat gccgatggat
     9901 tgctggagcc tgcacgcttg ctggcgacca aacgcgtggt agtaaagcgc ccggactacg
     9961 cgccaccgct agcgaatgtc gccacgccaa acgcggttgt gactaaaggg catcgctttg
    10021 atatctatgc aggtacgccc gtgtaaaagt aaagcagggt agcgataacg ctaccctgct
    10081 ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag ccacggcacc
    10141 atcaacagca tcactactgc aacgcccagc gtgaccagac caatcttacc aaacacgttg
    10201 gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt
    10261 gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat cccgagaata
    10321 aagcccatca aatgctgcgg caccagggca gcaatcatcg ccaggccaag ggcgctaata
    10381 aacagttcac ctaagctctg gaataagtac accagcacga taaaccatgg cgatgtcagc
    10441 ccttgtgcat ccgcaaacca cattcccgca gctgccgccg tcaaaaagcc cagtgagcac
    10501 ataaacatgc cgagagtaaa tttcatcggc atcgagaggt ctttgccttt gttacccaga
    10561 tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg gttaagcgcc
    10621 tggaagctga ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt gttgatggca
    10681 aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac cgcttcgagc
    10741 atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt gaatgcctga
    10801 cgaaagaaga tgatggtgac gacgatggag agaacaatca gcaccagatt ggcgacttct
    10861 acgttgtgca tcagccatgc acatacgaag atcatcacca cgctgccaag taatacgtac
    10921 aacagtttgc tgaagctcat cggcttgaag tcgggttcag aaccaatgtc tttcaccatt
    10981 ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ccgccccgca caggttgtag
    11041 gtgactgaat aaccgaatct atcagcgatc acaggggcca gcgataacgc tatcaacgag
    11101 ccgatattga tcgacatata gaacagggtg aatgcgccat caagccgcgg atctttcggc
    11161 ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt accgacagcg
    11221 atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag
    11281 tagccaatcg ccagcacaag tgctccaaga acaatggtgc gtttggtccc caacaggtgg
    11341 tcgccgacat agccgccaat ggaaatgagg ccatagacca gtgcagcaaa agcaccaaaa
    11401 gtgacaaaag cctgctcttg cgagaatcca agctgtttaa cgaagaaaac cgccagtacg
    11461 ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat catgaaaaat
    11521 gggcgaggtt gctgcagcat ccccatgggt gttgttgtat tcatatttat taaccttcca
    11581 ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc
    11641 agagattctt aatattgatg cggtggatag attaatacac tattacaaca gaaaataacc
    11701 agctgatcac actaatgatg gaaagtatga tggtgttaaa tgttgtattg gcaggagatt
    11761 ttgattgcgt acgcagatcg taggccagat aaggggttta cgctgcatca ggtaacaagg
    11821 cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg tggggggatg
    11881 attattcttc ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg gtgttgatca
    11941 gctgacgtgc ggaagacatt agtttgctcc agaagtcctg gtggtgacca caaaccacca
    12001 gatccatatc gtatttcttg attgcatcga ccagaacctg gcccaggtcg ccgctgccgc
    12061 tcagggtttc agtgattggg tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg
    12121 tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc ccggtgtata
    12181 ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtag gggcgagcca
    12241 tagagactgc tttctctacc agaactttgc tttccgggga gagatcgacc gcgatgagaa
    12301 tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg gccagctagc
    12361 gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc
    12421 cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga tccgtcaatc
    12481 cgccttgctt acccgttaga taaacaaatt ttcgacatga atatattgat agtggttaac
    12541 cttctggaaa aaaaacaacc tgatctccta cactatctat agagccgctc ggatgttggt
    12601 caccacaaaa gcggtttctc caggacctgg tgttaactga ttggcagcgt atcgggagcg
    12661 gtagccccgg ggtggaactc cgcgggcagg tcgccgggga ggaggtatga taagcaccgt
    12721 cgcattattt tgggctttat gtgtcgtttg cattgttaac atggcgcgct atttctcatc
    12781 actacgcgcg ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc aatatgttga
    12841 tggagggggc ttttttacct cacatggcca acccaacaaa caggtgcgtc tcgtttggta
    12901 tatctatgcc caacgttatc gcgatcatca cgatgatgaa tttattcgcc gctgtgagcg
    12961 ggtgcgtcgg cagtttattc tgaccagcgc attgtgtggt ctggtggtgg tcagcctgat
    13021 tgcattgatg atttggcatt aagcacttca gcaataataa agcataaaaa aagcgggcca
    13081 gtaaactgac ccgctcgaga ctgattacaa acgcaaaatt gcctgatgcg ctacgcttat
    13141 caggcctaca taatctctgc aatctattga atttgcatgt ttttgtaggc cggataaggc
    13201 gttcacgccg catccggcat gaacaaagcg cactatgtca caatctgaag cgggccagct
    13261 gacccgctcg ttttggtgcg tacgattaca ggaactgcaa ggagagccag tacagcccgc
    13321 cggaaagcag taccgcagcc ggaagggtaa acacccaggc catcagaatg ctggtcacgg
    13381 ttttacgctg taagccgcca ccatctacca ccatcgtccc cgcgacagaa gaggagagta
    13441 cgtgagtagt ggaaaccggc atcccggtat aactcgccag gccgatagac actgccgccg
    13501 tcatctgggc agacatcccc tgagcgtagg tcataccttt cttaccgatt ttctcaccga
    13561 tagtcgttgc cacacggcgc cagccaatca tcgtaccgat acctaacgcc agcgcgaccg
    13621 ccatgatgat ccacaccggt gcatactcga tggtgctaag catgtcggac ttcagtttct
    13681 tcaacaggcg ctgatcgtca gcactcacgc caggcatctt caccactttg tcgatagtgt
    13741 cagaaacgca cagcataatg cggcgcatct ggctacgttg atcaagcgac agcttgtcgt
    13801 agctttccac atcggtggtc aacatacctt tcaggcggtt gagcgcgtta atggtattcg
    13861 acggatggca gtggaactcc gcaggttgcg ttgcgccagc ttccggagcc ggtactaact
    13921 gatcagcacc ggtagcctgt ttgagcagcg caggatgctg ctcaaagtaa gcttcgacgt
    13981 tgttgatggc atcacgggta cgggtgattt cgtagccagt ggcattcatg ttcaccacga
    14041 agcctgctgg cgcgacgcca atcaatacca acataaccag accaatgcct ttctgaccat
    14101 cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag gatcagcgcg atacgcgtcc
    14161 agaacggcgg ctttttcttg ccgtctttct tttcacgctc cgctggggtc aggtggatac
    14221 gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa
    14281 acaccaggcc gacaataggg gaaacgatca gagaaccgaa aatacttaat actttcggga
    14341 tattgagtgc atccaccact gacgtcccgg tcatcaacgc attggttaaa ccaatcccga
    14401 tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa accaaagtac caggtaccca
    14461 ggttccagat aatcgccgcc agcaacatag agaacaccat ggcaaggcca tgagacgatc
    14521 ccatattaag cagcagatcc gtcggcagca tatgcacaat ggcataggca acactcagac
    14581 cacccagcaa aacacccaaa aagttgaaca ccgccgccat aaccacggcg agctgagaac
    14641 gcatcgcgcg ggtatagata acggttgcca cggcgttggc tgtgtcatgg aaaccattga
    14701 tggcttcgta gaacagcaca aaagccagtg caagcaataa taacagcccg gtatgcaaat
    14761 ccaggccagc aaacaaatgt agcataggac gttacgccat tttgaggaca tgaacgcggc
    14821 gcattatcag tgactttcgc ggcgcgggca aagtgaaata tagacttttt ttgatttgcc
    14881 tcctgtatgg atttcactca aaaaataatt atcttatata attcaggcaa atacttcctt
    14941 ttagtaatat tgatgctggt gcgaccactg aggaatcttt acaattcacg cccgtttttt
    15001 ctaagaggag cgcaacgtgg aaaggtttga tgccattatt ataggcgctg gtgcggcggg
    15061 tatgttctgt tctgcgctgg caggtcaggc aggacgccgg gttctgctga tcgataatgg
    15121 taaaaaacca gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact ttaccaacct
    15181 ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat ttttgtaagt ctgcactcgc
    15241 acgttttacc cagtgggatt tcattgatct ggtcaataaa cacggcatcg catggcacga
    15301 gaaaacgtta gggcaactct tctgcgatga ctccgcgcag cagattgtcg acatgctggt
    15361 ggatgagtgc gagaagggca atgtgacctt cagattgcgt agcgaagtgc tgagtgtggc
    15421 gaaggatgaa acaggcttca cgcttgatct aaacggcatg actgtcggtt gcgaaaagct
    15481 ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc gcatcgccgt ttggttataa
    15541 gattgccgaa caatttggcc tcaacgtgct gccgacccgc gcgggtctgg tgccgttcac
    15601 tctgcataaa ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt
    15661 gattaccgct gaaaacggca ccgttttccg tgagaactta ctcttcaccc atcgcggctt
    15721 gtctggaccg gcggtgttgc agatttccag ctactggcaa ccgggtgaat ttgtcagtat
    15781 caatctgcta ccggatgtgg acctcgaaac cttcctgaat gagcagcgta acgcacatcc
    15841 gaatcagagc ctgaaaaaca cactggcggt tcatctaccg aagcggttgg ttgaacgctt
    15901 acagcaactc gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgc gtgaccaaca
    15961 ggcactgatt agcacattga ccgactggcg cgtacaaccc aacggcactg aaggctatcg
    16021 cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa ctctcttcac ggacgatgga
    16081 agcgcgcaaa gtgcctgggc tgtacttcat cggcgaagtg atggacgtca ccggctggct
    16141 ggggggctat aacttccagt gggcgtggtc gagtgcatgg gcttgtgcgc aggatttgat
    16201 tgcagcgaag tcgtcctgac ctgttaccta aagatgaaaa ttattagagc cgcattaaaa
    16261 atgagctaat tttgatag
//
LOCUS       DAJSKJ010000079        16261 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000079 DAJSKJ010000000
VERSION     DAJSKJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.079"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            198..692
                     /gene="napF"
                     /locus_tag="ON345_002234"
     CDS             198..692
                     /gene="napF"
                     /locus_tag="ON345_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR3072291.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSSDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            682..945
                     /gene="napD"
                     /locus_tag="ON345_002235"
     CDS             682..945
                     /gene="napD"
                     /locus_tag="ON345_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR3072292.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            942..3428
                     /gene="napA"
                     /locus_tag="ON345_002236"
     CDS             942..3428
                     /gene="napA"
                     /locus_tag="ON345_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR3072293.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3435..4130
                     /gene="napG"
                     /locus_tag="ON345_002237"
     CDS             3435..4130
                     /gene="napG"
                     /locus_tag="ON345_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR3072294.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4117..4980
                     /gene="napH"
                     /locus_tag="ON345_002238"
     CDS             4117..4980
                     /gene="napH"
                     /locus_tag="ON345_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR3072295.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4977..5426
                     /gene="napB"
                     /locus_tag="ON345_002239"
     CDS             4977..5426
                     /gene="napB"
                     /locus_tag="ON345_002239"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR3072296.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5436..6038
                     /gene="napC"
                     /locus_tag="ON345_002240"
     CDS             5436..6038
                     /gene="napC"
                     /locus_tag="ON345_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR3072297.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVELGF"
     gene            6057..6674
                     /gene="ccmA"
                     /locus_tag="ON345_002241"
     CDS             6057..6674
                     /gene="ccmA"
                     /locus_tag="ON345_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR3072298.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6671..7333
                     /gene="ccmB"
                     /locus_tag="ON345_002242"
     CDS             6671..7333
                     /gene="ccmB"
                     /locus_tag="ON345_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR3072299.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7375..8112
                     /gene="ccmC"
                     /locus_tag="ON345_002243"
     CDS             7375..8112
                     /gene="ccmC"
                     /locus_tag="ON345_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR3072300.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8109..8318
                     /gene="ccmD"
                     /locus_tag="ON345_002244"
     CDS             8109..8318
                     /gene="ccmD"
                     /locus_tag="ON345_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR3072301.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8315..8794
                     /gene="ccmE"
                     /locus_tag="ON345_002245"
     CDS             8315..8794
                     /gene="ccmE"
                     /locus_tag="ON345_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR3072302.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8791..10734
                     /gene="ccmF"
                     /locus_tag="ON345_002246"
     CDS             8791..10734
                     /gene="ccmF"
                     /locus_tag="ON345_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR3072303.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10731..11288
                     /gene="dsbE"
                     /locus_tag="ON345_002247"
     CDS             10731..11288
                     /gene="dsbE"
                     /locus_tag="ON345_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR3072304.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKVLGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11285..12337
                     /gene="ccmH"
                     /locus_tag="ON345_002248"
     CDS             11285..12337
                     /gene="ccmH"
                     /locus_tag="ON345_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR3072305.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12372..13019)
                     /gene="narP"
                     /locus_tag="ON345_002249"
     CDS             complement(12372..13019)
                     /gene="narP"
                     /locus_tag="ON345_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR3072306.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13339..15930
                     /gene="yejO"
                     /locus_tag="ON345_002250"
                     /pseudo
     CDS             13339..15930
                     /gene="yejO"
                     /locus_tag="ON345_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3619 a         3978 c         4727 g         3937 t
ORIGIN      
        1 tttgaaatgt gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct
       61 acaggtttta ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct
      121 ataaaaatgg cttattaatt atgcggttta tttggccgct ctcaattttc agagcgcgtt
      181 aatgatggaa ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct
      241 ggcgcaaagc cagtaacggt atccgtccgc cttggtccag tgatgaatct cattttctga
      301 cccattgcac ccgttgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg
      361 gcgcgggcgg ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct
      421 gcgcgcaggc ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc
      481 agttcaccat tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg
      541 atagctgtga gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc
      601 aacttaatag ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag
      661 ccataactgc ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag
      721 gccaaaagcg aacgaatttc agacatcagc acccaactga acgcctttcc cggctgtgaa
      781 gttgctgtca gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc
      841 gaaacgctga tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg
      901 ctggtttatc accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta
      961 gctttatgaa agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg
     1021 gcgttgcccg cgccgttgtt ggtcagcagg aagccattaa atgggataaa gcgccgtgcc
     1081 gtttctgcgg tactggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct
     1141 gtcagggcga cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc
     1201 tgcccaagat catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg
     1261 gtaaatatga caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga
     1321 tggaagagaa attcaaaacc gccctgaaag aaaaaggacc ggaatcgatc ggtatgttcg
     1381 gttctggtca gtggactatc tgggaaggtt atgccgcgtc caagctgttc aaagcgggct
     1441 tccgttcgaa caacatcgac ccgaacgcac gtcactgtat ggcgtcggca gtagttggct
     1501 ttatgcgtac ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg
     1561 acgcgtttgt gctgtggggc gctaacatgg cggagatgca cccgatcctc tggtcacgca
     1621 tcactaaccg tcgtctctct aaccagaacg tcaccgtggc ggtgctttct acctaccagc
     1681 atcgtagctt cgagctggcg gataacggca tcatctttac gccgcaatct gacctggtga
     1741 tcctgaacta catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca
     1801 gcaagcacgt taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc
     1861 atccgctgga aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg
     1921 aagattacaa agccttcgtt gccgagtata cgctggaaaa aactgccgaa atgaccggcg
     1981 tgccgaaaga ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca
     2041 tctcctactg gacgatgggc ttcaaccagc atactcgtgg cgtgtgggcc aacaacctgg
     2101 tctacaacct gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc
     2161 tgaccgggca gccttccgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc
     2221 tgcctgcgga catggtggtg actaacgaga aacatcgtga tatctgcgag aagaagtgga
     2281 atatcccgag cggcaccatt ccggcgaaaa tcggtctgca tgcggtagca caagaccgtg
     2341 cgctgaaaga cggcaagctg aatgtttact ggaccatgtg taccaacaac atgcaggccg
     2401 ggccgaacat taatgaagag cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg
     2461 tctccgatcc gtatccgaca gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa
     2521 tgtgggtaga gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc
     2581 agcaggtaca ggcaccgggc gaagcgaaat cggatctctg gcagttagtc cagttctccc
     2641 gccgcttcaa aactgaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc
     2701 gtggcaaaac gctgtacgaa gttctgtatg ccacccccga agtgagcaaa ttcccggtat
     2761 ccgaactggc ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa
     2821 aagggctgtt cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt
     2881 tcgatgacta ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc
     2941 agtggcgtta cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tacaagttct
     3001 acggtaaacc ggatggcaaa gcggtgatct tcgcgctgcc gttcgaaccg gcggcggaag
     3061 caccggatga agagtacgac ctgtggctct ctaccggacg cgtcctggag cactggcaca
     3121 ccggcagtat gactcgccgt gtgccggaac tgcaccgcgc cttcccggaa gcggtcttgt
     3181 ttattcaccc gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa gtgaaagtgg
     3241 tttctcgccg tggcgaagtg atctcgattg ttgaaacgcg cggtcgtaac cgtccgccac
     3301 agggcctggt gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctgacgctgg
     3361 atgcgaccga tccgctctcg aaagagacgg acttcaagaa gtgcgcggtc aaactggaga
     3421 aggtgtaagc cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc
     3481 gcgatgttgt tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac
     3541 agcaaaccgc acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg
     3601 cctttgccag tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct
     3661 taaaactggc gacgctggcc tctggtctgt cggcgggcac gccatatttt gtcgcacggg
     3721 atattccttg cgaaatgtgt gaggacattc cgtgcgccaa agtgtgccca agcggtgcgc
     3781 tggatcgtga gattgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc
     3841 aggaaaactg tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga
     3901 aaattgatga ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc
     3961 gctttctgcc gacggttcac agcgacgcct gtaccggttg cggtaagtgc gaaaaagtat
     4021 gcgtgctgga acaaccggca atcaaggtgt taccgctgtc actggcgaaa ggggagttag
     4081 gtcaccatta ccgcttcggc tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc
     4141 gggcgcgagg cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt
     4201 cgcctttgtc agttcttcgt gctggggatg tttttgagcg gtccatggtt tggtgtgtgg
     4261 atcttgcacg gcaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg
     4321 ctgatgacgc tgcaaagtct tgccagtggt catctgcctg ccacggtggc gttgaccggt
     4381 gcggtgatta tcaccgtgct ctacgccctg gcgggaaagc gattattttg cagctgggtc
     4441 tgcccgctga acccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat
     4501 cagtctgcga cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctggtggga
     4561 tcagccttaa ctggcacgct catttgggaa tggatcaacc ccgtttctct gatggggcgt
     4621 agcctggtaa tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat
     4681 ctactggtcg ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggc
     4741 gtgctgggta gcaaaggtgt gattaccgtt gcggccaccg atcggcagaa atgtaaccgc
     4801 tgtatggatt gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat
     4861 gaacaaagcc cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat
     4921 gtctgttctg aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga
     4981 aaagccatga cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa
     5041 gcggcgcggt ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga
     5101 cacaggaagg ggccattcgg atgccaaaag agcaggaacg gatgccgctg aactatgtga
     5161 atcagccgcc aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc
     5221 gctgcttgca gtgccacggt gtcgaaagct atcgcaccac tggcgcgccg cgtatcagtc
     5281 ctactcactt tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt
     5341 tctgtctgca atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta
     5401 ccccatcaaa aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg
     5461 gtctgattaa gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc
     5521 tgctgttgat cggttttgtt ggcggcatcg tcttctgggg tggctttaac accgggatgg
     5581 aaaaagccaa taccgaagag ttctgcatta gctgccacga aatgcgcaac acggtgtatc
     5641 aggaatacat ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg
     5701 attgtcacgt tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag
     5761 agctgtatgg taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgtc
     5821 tgacgatggc acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta
     5881 actgtcacaa cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc
     5941 atgaccaggc ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca
     6001 agctgcccga tatgcgtgaa gtcgagctag gtttttaaca gggttattgc gtgggtatgc
     6061 ttgaagccag agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat
     6121 ttacgctgaa cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa
     6181 cgcttctccg tttgctgact gggttgtctc gccctgacgc tggcgaggtt ctctggcaag
     6241 ggcagccctt gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc
     6301 agccggggat caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg
     6361 gcgatgccgc acaatgtctg gaagccctgg cgcaggccgg gcttgccgga ttcgaagata
     6421 ttccggtaaa tcaactttct gccgggcaac aacgccgcgt cgctttagct cgtctgtggc
     6481 tgacccgtgc cacgttatgg atcctcgacg agccttttac cgcgattgac gttaacggcg
     6541 tcgatcgtct gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga
     6601 ctacccacca gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa
     6661 cgggggccgc atgatgttct ggcgcatttt ccgtcttgag ctgcgcgtag cgtttcgcca
     6721 tagcgccgaa atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct
     6781 cagtatcggt ccggagccgc aactgctggc gcgtattgca ccgggcatta tctgggttgc
     6841 tgcgctgctt tcatccttgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg
     6901 cagtcttgaa caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt
     6961 gatggcgcac tggatggtaa ccggtctgcc gttactcatc ctttcgccac tggtagcaat
     7021 gctactggga atggatgttt atggctggca agtgatggcg ctgacgctgc tgctgggaac
     7081 gcctacgctt ggctttctcg gtgcaccggg tgtggcgttg actgtgggac ttaagcgcgg
     7141 tggtgtgctg ctcagcatac tggtgttacc gctgactatc ccattactca tctttgccac
     7201 cgccgcgatg gacgcggctt ccatgcattt gcccgttgac gggtatctgg caattttagg
     7261 cgcgttgctg gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat
     7321 cagcattcaa taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg
     7381 aaaacactgc atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata
     7441 ccgtggctgg caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc
     7501 tttgctccgg ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct
     7561 gcggcgatct ggtcgatggg catttatgca tcaatggcag tggcagcgtt tattggcctt
     7621 gtctggcaga tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg
     7681 tttaccttta ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg
     7741 tgggtatggg atgcacgtct aacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg
     7801 attgccctgt ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg
     7861 gtgctgattg gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc
     7921 ctgcatcagg gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg
     7981 cgctggtcga tttttggctt cctgctcctg tctgccacgc tgacgctgat gcggatgcgt
     8041 aatttgattt tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga
     8101 ggccgtaaat gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg
     8161 ccttttttgt ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc
     8221 actcggtgat gcaacatcgc gcaattctgc gtggtgtggc gcaacagcgg gcgcgtgagg
     8281 cgcgtttacg tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt
     8341 gtggattgcc tgtgccgtgt tggcagggct ggcgctgact atcggtctgg tgctatatgc
     8401 gctgcgctcg aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga
     8461 aactcagcaa atgccggaag tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg
     8521 tagcgtgcag cgcgatccca attcgctaaa agtaactttc accatttacg acgctgaagg
     8581 ttcagtagat gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt
     8641 tgtggtgcag ggcgaactgg aaaaaggcaa tcatatcctc gcaaaagaag tgctggcgaa
     8701 acacgacgaa aactacacgc cgccagaagt tgagaaagcg atggaagcca atcaccgtcg
     8761 cccggcgagt gtttataagg acccagcatc atgatgccgg aaattggtaa cgggctgctg
     8821 tgtctggcgc taggaattgc gctgctgctg tccgtgtatc cgctatgggg cgtagcgcgc
     8881 ggagatgcgc gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg
     8941 gctggcgcat ttctggtact ggtcaatgcc ttcgtggtca acgacttcac cgtcacctat
     9001 gttgccagca actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggc
     9061 gcgcatgaag gctcgctcct gctgtgggtg ctgctgatga gcggctggac ctttgcggta
     9121 gcgattttta gtcagcgtat tccgctggat attgtggccc gcgtactggc gataatgggg
     9181 atggtcagcg tcggcttttt gctgttcatt ctctttacct ctaacccgtt ctcacgcacg
     9241 ttgccgaact tcccgattga agggcgcgat cttaacccgc tgttacagga tccggggctg
     9301 atcttccatc cgcctctgct ctatatgggg tacgtggggt tctcggtggc gtttgctttt
     9361 gccattgctt ctttgttgtg cgggcgtctg gacagcactt atgcgcgttt tactcgtccg
     9421 tggacgctgg cggcgtggat cttcttgacg ctcggcattg tgctcggttc cgcatgggcc
     9481 tattacgaac tcggctgggg cggctggtgg ttctgggatc cggtagaaaa cgcctcgttt
     9541 atgccgtggc tggtggggac tgcgctgatg cactcactgg cggtcactga acaacgcgcc
     9601 agcttcaaag cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgctgggg
     9661 actttcctgg tgcgttccgg cgtgctggta tcggtacacg cgtttgcctc tgatccggca
     9721 cgcggtatgt ttatcctcgc ctttatggta ctggtgattg gtggttcgct gctgctgttt
     9781 gccgcgcgtg gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct
     9841 ctgctgttag cgaacaacgt tttgctggtc gccgcgatgc tggtggtatt actggggacg
     9901 ctgctgccgc tggtgcacaa gcaactggga ctgggcagta tttcgattgg cgaaccgttc
     9961 ttcaacacca tgtttacctg gctgatggtg ccgtttgcgc tgctgcttgg tgtcggtcct
    10021 ctggtgcgct gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc
    10081 atctcaacgc tggtgctgtc gctgctgctg ccgtggctgt tcgaaagcaa agttgtggcg
    10141 atgacggtgc tcggcctggc aatggcctgc tggattgctg tgctggcaat tgcggaagct
    10201 gcgctgcgta tttcacgtgg cacgaaaacc accttcagtt attggggaat ggtggcggct
    10261 cacctggggc tggcagtgac aattgttggt attgccttta gccagaacta tagcgttgag
    10321 cgtgatgtgc gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc
    10381 cgtgatgtca aagaggtgac tggcccgaac tggcgtggcg gtgtggcgac tatcggcgta
    10441 acgcgcgatg gcaagccgga aacggtgctg tatgcggaaa aacgttatta caacactgcc
    10501 gggtcgatga tgaccgaagc ggcgattgac ggcggcatca cgcgtgacct gtacgccgcg
    10561 ctcggtgaag agctggaaaa cggcgcgtgg gccgtgcgtc tttactacaa accgtttgtt
    10621 cgctggattt gggcgggcgg gctgatgatg gcgttgggcg gactgctgtg tctgtttgat
    10681 cctcgctatc gtaagcgcgt gagtccgcaa aaaactgcgc cggaggctgt atgaagcgca
    10741 aagtattgtt aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagc
    10801 tggcgcgtaa tgccgaaggg gatgatccga ccaatctgga atcggcgctc attggcaagc
    10861 cagtgccgaa gtttcgcctc gaatcactgg acaatccggg gcagttttat caggcggatg
    10921 tgctgactca gggcaaacct gtgctgctta acgtctgggc gacctggtgt ccgacctgcc
    10981 gtgcggaaca tcaatatctg aatcagcttt ctgcgcaggg cattcgcgtg gtcggcatga
    11041 actataaaga cgatcgccag aaggcaatca gctggctgaa agtgctgggc aatccttacg
    11101 cgctaagcct gtttgatggc gatggcatgt tagggctgga tctcggtgtc tatggcgcgc
    11161 cagaaacgtt cctcatcgac ggcaacggca tcattcgcta tcgccatgcg ggcgatctca
    11221 atcctcgcgt ctgggaagaa gagatcaagc cgctgtggga gaaatacagt aaggaggccg
    11281 cacaatgagg tttttattgg gcgtgctgat gctgatgatc tccggctcag cgctggcgac
    11341 catcgacgtg ttgcagttta aagatgaagc acaggaacaa cagttccgtc agctcactga
    11401 agaactgcgc tgcccgaaat gccagaataa cagcattgcc gattccaact cgatgattgc
    11461 caccgacctg cgccagaaag tgtatgaact gatgcaggaa ggtaaaagta agaaagagat
    11521 tgtcgattat atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc
    11581 gctgaccgtg ctgctgtggg tgctgccagt agtggctatt ggcattggcg gttgggtcat
    11641 ttacgcccgc tcgcggcgtc gggtacgcgt ggtgccggaa gcgtttcctg aacaaagcgt
    11701 gccggaaggt aagcgtgccg gatatgtcgc ttatttgccg ggcattgtgg tggcgttaat
    11761 tgtggctggc gtcagctact accagaccgg aaattatcaa caggtgaaaa tctggcagca
    11821 ggccacggca caggctccgg cgttgctgga cagggcgctg gatccgaaag ccgatccgct
    11881 caatgaagaa gagatgtcgc gtcttgcgct ggggatgcgt actcaactgc aaaaaaatcc
    11941 gggagatata gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgctag
    12001 tatcgccacc gatgcatacg ctactgcgta tcgcctcgat ccgaaaaaca gcgatgccgc
    12061 actgggttat gctgaagcgt tgacacgttc atctgatccc aacgacaacc gcctcggcgg
    12121 cgaactgctg cgccagttgg tgagaagcga ccacagcaat atccgtgtgt taagcatgta
    12181 tgcgtttaat gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat
    12241 gttgaaactc ttacctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgca
    12301 ggcgatgcaa catttgtcgc cgcaggagag taaataaaaa gaggcccgat ggtgatgcca
    12361 tcgggctatt tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc tgccacgcgt
    12421 gagcggacat tgagtttgcg cagcagattg cgaatatgta cttttactgt ctgctctgaa
    12481 atattcaaca ccgaggcaat ctgtttattt gacagcccct gtgccagctc gtgcagaaca
    12541 tccagctcgc gctccgtcag cacgctgaag ggatcttctt ccgcgccaaa catttcacgt
    12601 tcacgtaagt actgattgac gcgttcgcta aagactttgc tgcctttcgc tccagcacga
    12661 attgcttcca gcaatacttc cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg
    12721 tctatcagcg caaagacatc gctggaggca tctgatacgg tcaggataat aatttgcgcg
    12781 gtaacgccat ccctgcgcaa ggcattgagg gtatccaggc cactcatacc tttcatattg
    12841 agatccagca agatcacgtc gatatccagt ctattcgcca gatcgatcgc gctcgcgccg
    12901 tcgcccgctt cggcgaccac ttcaaagcca ggatcaagct ccagtaactg acgaacaccg
    12961 cgtcgcataa gtggatgatc atccacaatc atcacctgaa aaggcgttgc ttcaggcata
    13021 gtagtctcct gaggttttat tagaatcatt atgtttgagc gtagcgttgg taacgctacc
    13081 aggcgcgtga atgttacccc attttatgta tggacatgta gtgaaaacta caaccttgtc
    13141 gttcgctttg gcatcatttt cattataaag acggaatttt gcatcagctt ctcatcaaga
    13201 aaaacaacta aaaaatcaat cacaatactt gtgcatttct ttaaacagtt tttaaaaact
    13261 gactccgggt atggagctat gggtattttc tgtacccaat gcttttaact gcaattaatt
    13321 tcataggatg aaagctcaat gcatcaatct ggttctgttt ctctttgtcg ttccgcaata
    13381 tctgttctgg tggctacagc gttatattca cccatagcat tggcatcaac agttgagtat
    13441 ggtgagacag ttgatggtgt tgtcctggaa aaagatatcc agctggttta tgggaccgcc
    13501 aataatacga aaatcaatcc tggcggagaa cagcatataa aagaatttgg tgtaagtaat
    13561 aatactgaaa ttaacggagg gtatcagtac attgaaatga atggcgccgc agaatactca
    13621 gtattaaatg acggttatca aattgttcaa atgggtggcg cggcaaacca gactacgctc
    13681 aataatggtg tgctacaggt ttatggcgca gcgaatgata ccacgattaa aggcgggcgc
    13741 ttaatcgttg aaaaagatgg gggggccgtc tttgtcgcta tcgaaaaggg aggactactg
    13801 gaggttaaag agtggggatt agcgattgcg gtagatcaga aagcaggcgg tgctattaaa
    13861 acaaccacgc gggccatgga ggtattcgga acaaactgtc tcggtcagtt cgatatcaag
    13921 aatggtattg ctaataatat gttgttggaa aacggcggaa gtttgcgagt tgaagaaaat
    13981 gacttcgctt ataataccac tgtagatagt ggcggcttac tggaggttat ggatggcggg
    14041 actgtaactg gcgttgataa aaaagcaggc ggaaaattaa ttgtctcaac gaatgcgctg
    14101 gaagtgagtg gtccaaacag taaaggccaa tttagtataa aagatggtgt gtcaaaaaat
    14161 tatgaactgg atgatggttc cgggctcatt gttatggagg acacgcaggc cattgatact
    14221 atccttgatg agcatgccac tatgcaatcg ctgggaaagg atactggtac gaaagtgcag
    14281 gcaaatgcgg tatatgatct cggtcgatca tatcagaatg gaagtatcac gtattcctca
    14341 aaagccatct ctgaaaatat ggttatcaac aatggccgcg ctaacgtctg ggctggcaca
    14401 atggttaacg tttcagtcag agggaatgat ggcattcttg aggtcatgaa gccgcaaata
    14461 aattatgcac ccgcaatgtt ggtgggtaag gtagtggttt ctgagggcgc ttcttttaga
    14521 acgcatggtg ccgtggatac cagcaaagcg gacgtttcgc tcgaaaatag cgtatggacc
    14581 atcattgccg atatcactac gacgaaccaa aacaccctcc tcaacttagc caaccttgcg
    14641 atgtctgacg caaatgtgat tatgatggat gagccagtga ctcgttcatc agtgacggca
    14701 agtgcggaaa atttcattac gttgaccacc aataccctgt cgggaaacgg caatttttat
    14761 atgcgtaccg atatggctaa tcatcagagc gatcagctca acgtcaccgg tcaggcaaca
    14821 ggtgatttca aaatattcgt gacggacacc ggtgccagcc cggcagcagg agatagcctt
    14881 acactggtaa caacgggcgg cggtgatgct gcatttacgt tgggcaatgc cggaggcgtt
    14941 gttgatatcg gtacgtatga atataccttg ctggataatg gcaaccatag ctggagtttg
    15001 gcagagaatc gcgcgcaaat taccccttca accactgatg tgctgaatat ggcggcagca
    15061 caaccgctgg tatttgatgc agaactggac accgtgcgtg agcgtcttgg tagcgtaaaa
    15121 ggcgttaatt acgatacggc gatgtggagt tcggcaatta acacccgcaa caacgtgacc
    15181 actgatgcgg gcgctggctt tgagcaaaca ttgaccgggc tgacgctcgg tatcgatagc
    15241 cgtttctccc gtgaagaaag cagcacaatt cgcggcttgt tctttggtta ctctcattct
    15301 gatattggtt ttgatcgcgg cggcaaaggc aatgtcgata gctataccct gggggcttat
    15361 gccggttggg agcatcagaa cggtgcctat gttgatggag tggtgaaagt tgaccgtttt
    15421 gccaacacca tccatggcaa gatgagtaat ggggcaacag cgtttggcga ttacaatagt
    15481 aacggcgcgg gtgctcatgt cgagagcggg ttccgttggg ttgacggatt gtggagtgtt
    15541 agaccctatc tggcctttac cggctttacc acagatggtc aggactacac gttatcaaac
    15601 ggcatgcgcg cggatgtggg aaatacccgg atattacgcg ctgaagcggg aacggcggta
    15661 agctatcaca tggacctgca aaacggtacg acgctggaac cctgactgaa agccgccgtg
    15721 cgtcaggaat acgccgattc taaccaggtg aaagttaatg acgatggcaa atttaataat
    15781 gatgtggctg gaacccgtgg cgtttatcag gctgggataa ggtcatcgtt taccccgacg
    15841 ttaagcggtc atttgtcagt cagctatggc aatggcgcag gggtagaatc gccgtggaat
    15901 actcaggcgg gtgtggtctg gacgttctga taacaaaaaa taaacaggct gtgatgtgtc
    15961 acggtctgtt tatcgaatta attgcagata taaaaaaaac caaccgtaaa ggttggtttt
    16021 ttcttgggat ttttggcacg agagtagttt aattgagcct gtaactgcat gattttaaat
    16081 atgtagatta attgagtttt gaaatcgtat atccaaacgt ataactaaag tgatttgctg
    16141 aaaaaatgta cagctttaac cgttatcatt ttccggctat ccgctatgaa gctggctggt
    16201 ggatgatcac ggataacctg acatctacag ccgccttaaa aagctgaacc cgacaagcat
    16261 a
//
LOCUS       DAJSKJ010000080        16178 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000080 DAJSKJ010000000
VERSION     DAJSKJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.080"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..894)
                     /locus_tag="ON345_002251"
     CDS             complement(<1..894)
                     /locus_tag="ON345_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3072307.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFYLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIIST"
     gene            complement(996..1976)
                     /locus_tag="ON345_002252"
     CDS             complement(996..1976)
                     /locus_tag="ON345_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR3072308.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMQSLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1984..2694)
                     /locus_tag="ON345_002253"
     CDS             complement(1984..2694)
                     /locus_tag="ON345_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR3072309.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDISQTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSSVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2911..4734)
                     /gene="typA"
                     /locus_tag="ON345_002254"
     CDS             complement(2911..4734)
                     /gene="typA"
                     /locus_tag="ON345_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR3072310.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5107..6516
                     /gene="glnA"
                     /locus_tag="ON345_002255"
     CDS             5107..6516
                     /gene="glnA"
                     /locus_tag="ON345_002255"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR3072311.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6690..7739
                     /gene="glnL"
                     /locus_tag="ON345_002256"
     CDS             6690..7739
                     /gene="glnL"
                     /locus_tag="ON345_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR3072312.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7751..9160
                     /gene="glnG"
                     /locus_tag="ON345_002257"
     CDS             7751..9160
                     /gene="glnG"
                     /locus_tag="ON345_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR3072313.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9272..9382
                     /locus_tag="ON345_002258"
     CDS             9272..9382
                     /locus_tag="ON345_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR3072314.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9572..10945)
                     /gene="hemN"
                     /locus_tag="ON345_002259"
     CDS             complement(9572..10945)
                     /gene="hemN"
                     /locus_tag="ON345_002259"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR3072315.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11134..11643)
                     /gene="yihI"
                     /locus_tag="ON345_002260"
     CDS             complement(11134..11643)
                     /gene="yihI"
                     /locus_tag="ON345_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR3072316.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12225..12857
                     /locus_tag="ON345_002261"
     CDS             12225..12857
                     /locus_tag="ON345_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR3072317.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13239..16025)
                     /gene="polA"
                     /locus_tag="ON345_002262"
     CDS             complement(13239..16025)
                     /gene="polA"
                     /locus_tag="ON345_002262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR3072318.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         3881 a         4183 c         3988 g         4126 t
ORIGIN      
        1 ggtactgatg ataaaggtgt agcatagata tttactcggc gatttcagga tcttatctga
       61 aatcatgcca ccaatcggtc caccaatcat tttcagacag tattggttaa tgatgccgta
      121 agcccccacc agcgcaacgg gcaatagata gatgtttttc aggaatggaa tgaagaatgt
      181 caggccacag taaaccgcgt aaacaaagaa gacgttaaaa gcgataagcc agatggtttt
      241 gtctctcagc accgaggtca tactggtgtt ttgcgatgct ccgtcttctt ttttcacctc
      301 aacggacggt gcctcttctt tgtcattcag gacaaagaaa ataataatgc ctacggcaat
      361 cacaataagg gaatagaacc agatgcctgc tttaaaacct aataagccac tgccaaacca
      421 ggtaaatact gccaacgcag aaaatgccac cacggtatcg acaatgccac gccctgtttc
      481 gaagaagcca aacaaccgac cttgttgttc gctattgccc aatcgactta ccgacttgag
      541 caagaccggc cagttcatca tgtcgcaagt aacgccaaat agcgcccaga caaagaggat
      601 gccccaatac cccggcattg tcgtcaaata aacacccagt aatcctgtcg caatgagtga
      661 gaatgacatg gtgtatctgc gcggtagttt atcggcaaaa taaatagaca gaaaaaagcc
      721 cactgtggtg acaaatgagt ttaccgacat agcattacca atttgaccat tggtcaaata
      781 gaaatattcc tgcatcggga tataaaacgc atctttcagc gacggtaatt tataaattgt
      841 accgccacac agtgttaata gactaaataa cgcccatttc tttttcgtga gcataacatc
      901 ccctaagcga tttttaaaat cactgtttta aaggcaataa gataccgacc attcatatgg
      961 aatggtttta ttaactgcgt atgagttaaa acttattagt ggttcagaag atggtttgcc
     1021 tcttgtttga tttgttgtag tacgttacgc acatgattta tctgattaat tgcatcttct
     1081 ttttctttac gtaaccgcgc atccagtaaa tgattttctg gcaatggtgt ttcactcatc
     1141 tggcgataag ggatccacgg attatcatct tcgtcttcaa agcggaacgc cggcgcatag
     1201 taatcaacct cttcttccag gccatatgcc gtcacctgcg gctcatcatc gcccaggcag
     1261 ataaggtgcg ttaataacgc tttgaacggc atatcaccct gacctgaaat acaggcttta
     1321 tgacccaggc caccctgttc tttaacgatc aaggcatctt tgatatggac ctgggtaata
     1381 tgcggtgcca tcgtttttaa ggcgtctatg ggatgctcat ttgcattaat catgttcgca
     1441 aaatcaaaca gtaaggaaag agactgcatc tcactttctt tgaccagcga caccagctca
     1501 tgacttttta aatcttcatg ctgctcgatc gtaaaagtca ggccgctgtc ctgatacgtt
     1561 tcccgtacat aggcaatgtc gttagcgata atcgataata cgtcgcgcaa attaccttca
     1621 taacgtggat aaaaacgtac ggacgatgcc ccagttttca acgcgatggc gacggcttcg
     1681 tcaatagatg ccttatctga ggcgctggtt tcaatatgaa tatcaaggtt cagacggcgg
     1741 gctttatctc caaaggcaga gagttcttta tcgtccatat taccaagaga aaaacgctcg
     1801 ccatccagaa catggatttt gacgccacgt agattatttt cacttgcgat atcaagtaaa
     1861 tcagcaggca acactctttc cagccgcata tttaaatgaa aggcataagc atgtaaataa
     1921 agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt tatttattgt caccataaaa
     1981 cacctacaga ttaatgatga ggtctacggc ctcaaatcgc caatattcaa tatctatctg
     2041 gacaatattg ccttgctggt cggcgcgatg cttttcaatt aagatcgcag gcatacccgc
     2101 agaaccgcca agatacttgc tgatatcgcc cggcattctg acgggtttga aaatcaattt
     2161 ttttactacc gtctcttttt ggtagcactt ttcccagact gaagaaaatg agtggttttc
     2221 aagttgttca ataaaacctg gggcaacttc aggattaata tatgtttcat gatagaaaac
     2281 tttatgtcct tccagcgccc cccagccggt aatacgatag agttcggttg atggcgtcac
     2341 tgcaatcagt ggcgcaagcg tctgggaaat gtcgctggta cggcttttct cggtaaaccc
     2401 ccaggaaggt tcccgccctt gctcaattgc ggcccgctga aagctcgccg acagctccgg
     2461 gctgtaatta aatcgcggct gagtcacgaa ccacccttta cgatccttac ggaagatttt
     2521 ggattctgcc tcgaggttca gcaacgcctg gcgcagcgtc atacgtttaa tgcccagcag
     2581 ctctcccagc tctctttctg aagggagttt tccacccggc gtagtgatgc catcttttaa
     2641 ccaccgatcc agtttctctt ttgcattttc tacggtggat tgatttcccg tcattgttta
     2701 atccttttgg tttaaaccag ataaggcatc ttacgcttac gccaaaaaag aaagcaatta
     2761 acggcgtatg actttgcaga taattgtgac aaagctctct attgacgtaa ccacagactt
     2821 ataagggaga tactcgcatt gctgagtgct cacgacaggc aaaaaaaaac ctgccatcgc
     2881 tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct ttcggtgcgc ggttggcgcg
     2941 acgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggtcg gggtcacttc
     3001 taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc ggataggcgg
     3061 aaccagaaca acagcttcgt cagtaccgga agcacgcatg ttggtcagtt tcttaccggt
     3121 caggcagttt acagtcaggt cgttagagcg gctatgaata ccgataatct gaccttcgta
     3181 aacttctgca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga acagcgcgaa
     3241 cgcgaccgct ttaccctgac cgttagagat cagtacaccg ttctgacgct gacccacttc
     3301 gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagaccag taccggaagt
     3361 catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca cgtagtcgag
     3421 acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac gttcgcccag
     3481 cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc agagtcacgt tttcatatgg
     3541 ctcttgttta cgaccgtcga tttcacggaa gataactttc ggacgggata ccgccagttc
     3601 gaaaccttca cgacgcatgt tttcgatcag aacagacagg tgtagttcgc cacgaccaga
     3661 aacgcggaac gcatcagcgt cttcggtttc ttctacgcgc agcgcaacgt tgtgtaccag
     3721 ttctttgttc agacgatcca ggatctgacg agacgttacg aacttacctt ctttaccgca
     3781 gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa cggagagtgc
     3841 cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca gttcgccaag
     3901 gcccgtgatc gcaacgatat cgccagcttc cgccaggtcg gtttcgatac gttccagacc
     3961 gaggtggccc agcactttac cgactttcgc gttgcgggtt ttgccttcgc tatcgatgat
     4021 agtgacctgc tggttcggct tcactttacc gcgcttgatg cggccaatgc cgataacgcc
     4081 aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt caaggtcaac
     4141 gtccggcgca ggaacgtggt caacaatcgc ctggtacagc ggggtcatgt cttccgccat
     4201 atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga tcgggaagtc
     4261 cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat ccacaaccca
     4321 atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct tcaggccgta
     4381 agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa atgcgtcaac
     4441 caccagcagc actgagtcta ccatggacat tacacgttca acttcaccac cgaagtcggc
     4501 gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag cggtgttttt
     4561 cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca cgcgctcttg
     4621 ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta ccagggtggt
     4681 tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga tcacaacttt
     4741 gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta caaaacagga
     4801 tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct ttcacggagc
     4861 ataaaaaggg ttatccaaag gtcattgcac caacatggtg cttaatgttt ccattgaagc
     4921 actatattgg tgcaacattc acatcgtggt gcagcccttt tgcacgatgg tgcgcatgat
     4981 aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt ttttacggcg
     5041 acacggccaa aataattgca gatttcgtta ccacgacgac catgaccaat ctaggagagt
     5101 taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt gaagtttgtt
     5161 gatttgcgct tcaccgatac taaaggtaaa gaacagcacg tcactatccc tgctcatcag
     5221 gtgaatgctg aattcttcga agaaggcaaa atgtttgacg gctcctcgat tggcggctgg
     5281 aaaggcatta acgagtccga catggtgctg atgccagacg catccaccgc agtgattgac
     5341 ccgttcttcg ccgactccac cctgattatc cgttgcgaca tccttgaacc tggcaccctg
     5401 caaggctatg accgtgaccc gcgctccatt gcgaagcgcg ccgaagatta cctgcgttcc
     5461 actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct gttcgatgac
     5521 atccgtttcg gatcatctat ctccggttcc cacgttgcta tcgacgatat cgaaggcgca
     5581 tggaactcct ccacccaata cgaaggtggt aacaaaggtc accgtccggc agtgaaaggc
     5641 ggttacttcc cggttccacc ggtagactcg gctcaggata ttcgttctga aatgtgtctg
     5701 gtgatggaac agatgggtct ggtggttgaa gcccatcacc acgaagtagc gactgctggt
     5761 cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga aattcagatc
     5821 tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta aaaccgcgac ctttatgcca
     5881 aaaccgatgt tcggtgataa cggctccggt atgcactgcc acatgtctct gtctaaaaac
     5941 ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt ctgagcaggc gctgtactac
     6001 attggcggcg taatcaaaca cgctaaagcg attaacgccc tggcaaaccc gaccaccaac
     6061 tcttataagc gtctggtccc gggctatgaa gcaccggtaa tgctggctta ctctgcgcgt
     6121 aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg tcgtatcgaa
     6181 gtacgtttcc cggatccggc agctaacccg tacctgtgct ttgctgccct gctgatggcc
     6241 ggtcttgatg gtatcaagaa caagatccat ccgggcgaag ccatggacaa aaacctgtat
     6301 gacctgccgc cagaagaagc gaaagagatc ccacaggttg caggctctct ggaagaagca
     6361 ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg gtggcgtgtt cactgacgaa
     6421 gcaattgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg tatgactccg
     6481 catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta ctacccggcg
     6541 caacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
     6601 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
     6661 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
     6721 agatcctcaa ctcgctgatt aacagtattt tgttaatcga tgacaacctg gcgatccatt
     6781 acgccaaccc tgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacac
     6841 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
     6901 aggcggggca aggttttacc gataacgaag tgacgctggt catcgacggg cgctcgcata
     6961 tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag atggctccga
     7021 tggataacca gcgccgctta agtcaggaac agctacagca cgcccagcag gttgctgccc
     7081 gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg
     7141 cggcgcagct gctcagcaaa gcgttacctg acccatcact actcgaatat accaaagtga
     7201 ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg tctgttgggg ccgcagctgc
     7261 ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta acgctggtgt
     7321 cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc ctgccggaac
     7381 tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc aatgcgctac
     7441 aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg tttcaactga
     7501 ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat aacgggccgg
     7561 gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg
     7621 gcaccgggct tggcttatcc atcgctcgta atttgattga tcagcattca ggcaaaattg
     7681 aatttaccag ttggccaggg cataccgagt tctcggttta cctgcctatc aggaaataaa
     7741 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
     7801 ggtgcttgaa cgtgcgctcg ctggggcagg tttaacctgt acgacgtttg agaacggcgc
     7861 agaagtgctg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
     7921 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc caatgcttcc
     7981 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
     8041 ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc tggttgagcg
     8101 cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta acggcccaac
     8161 gaccgatatc atcggcgaag cgccagccat gcaggacgtg ttccgtatta tcggtcggct
     8221 ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt
     8281 cgctcatgcc ctgcatcgcc acagtccgcg cgccaaagcg ccgtttatcg cgctgaatat
     8341 ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga aaggcgcgtt
     8401 tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg gtacattatt
     8461 cctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
     8521 agacggtcag ttttaccgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
     8581 cgctgccact caccagaatc tcgaacagcg agtgcaggaa ggtaagttcc gtgaggatct
     8641 gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac gtcgggaaga
     8701 tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg gcgtagaagc
     8761 gaagttactg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc caggcaacgt
     8821 gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc aggaagtgtt
     8881 gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat
     8941 gcaaccggac agctgggcga cgcttcttgc gcagtgggca gacagagcgc tgcgttccgg
     9001 tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac tgacgaccgc
     9061 gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct ggggccgcaa
     9121 caccctgacg cgtaagttaa aagagctggg gatggagtga ttcacagctt gtgtgtaaag
     9181 attgattatt gagcgcaaat tgctggtatt ttacgcttta ctgttccgat aagttcagta
     9241 tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc
     9301 gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc
     9361 aatgattggg ctgtttagtt gagtttcaga ttgctgatga agtgctcttt gttcatgccg
     9421 gatgcggcgt gaacgcctta tccggcctac aaaagcaata ttttcaacag gttttgtagg
     9481 cctgataagc gtagcgcatc aggcaatctt gttctcagca cattgatggc gctttcgttt
     9541 tctactttgt aaacgaagcg ccattcacta tttaaatcac ccgtgagaac tgctgcatcc
     9601 gcgctttctg gcgcagatag gtatcaaagc acatgcaaat gttgcggatc agcaagcgac
     9661 ctttcgccgt cacctgtatt cccttctcat ccacatccac cagcccatct tttgctaacg
     9721 gggcgagcag cttgagatct tccgcaaagt aatcagcgaa gtgcaaatcc cactgtttct
     9781 caataggggc gtaatccaga cggaagttgc agatgagcga cttaatcaca tcgcggcgaa
     9841 tacagtcatc acgcgttagc gcaataccac gccacagcgc attgccttgt tcatccactt
     9901 gctgatagta ctgcttcaac tctttctggt tctgcgcgta gcagtcgcca atcatgctga
     9961 tggcggaaac gcccatcccc agcagatcgg tatcgccctg agtggtgtag ccctggaagt
    10021 tacgatgcag cacgccttca cgctgggcca ccgccagctc atcatccgga cgggcaaagt
    10081 gatccatacc gataaactga tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga
    10141 tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa
    10201 aaatggtcgg cagatgcgcg tagttaaaga cactcagacg atcggggttc agctccgcca
    10261 cacgtttcag ggtaaaggcg aaactctccg gcgtctgttt cggcaggccg taaatcaggt
    10321 cgatgttggt ggaggtaaaa ccaatctcac gcgcatggtt aagcagtgca aagatgaact
    10381 cttcatcctg ctcgcggtta accagacgtt gcacttcttt gttgaagtcc tgcacgccca
    10441 tgctcaggcg attaaagcct tcggcgcgta aatgatcgag tacatccagt tcgatttccc
    10501 gcggatcgac ttcgatcgaa atctccgcat cggcattgaa ctggaagttt tcgcgcagca
    10561 gcttcatcag gcggctgatt tgcgctttat tcagatacgt cggcgttccg ccgccccagt
    10621 gcaattggct gacgtgacgc ccggcgaaca gcggtgcacg atggacgatt tcttgctcca
    10681 gcgcgtccag atactgatcg gccttgtgct gctggcgagt aacaatctta ttgcaaccgc
    10741 agaagtaaca aagcttatgg cagaacggga tatgtacgta gagagataat ggacgctcag
    10801 gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg
    10861 cggtcgggta cgaggtgtat cgtggcccgg aatagttata tttctggatc agggccagat
    10921 cccagtcgat ttgctgtaca gacatgctca ctccttccga tggcgtctct ggcggctacg
    10981 gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg cgcagccact tttgtcgccg
    11041 cgacaaccgc tgtagtttaa cgaataacca caccagataa catataaccg gaagggttat
    11101 cagcagaacg ggaaggccca cggcggcaat ccgttagttg ccccgcagca gacgcatcat
    11161 gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga ggccgagttt
    11221 ctgcatcaac tcatcaatac gatccagttt agcatccacc caggattgct cttcggcact
    11281 cagggtttcg cctgcttcca gacgttccag cagcgcatcc agacgctcat ccgtttccag
    11341 taattccaac tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt
    11401 ggtgactttt tcagtcacgc ccaatggaat aggggtttta ctgccaatac gtggatcttt
    11461 tggtgcgttc tggcctttgc tgcctgacgt ggtgtttccg cccgctgcgc ggctgcccgg
    11521 cgcatgacca cgacgttttt tctgacgctt gcgatcacga gcttcctgat ccagctcctc
    11581 gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag atgatggttt
    11641 cataatggtt cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga
    11701 aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg actcataacc
    11761 cttaacgggt cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc tttagccgga
    11821 acaatccgtg ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt cttcctgaca
    11881 atcctctgtc ttcgcctcct ggcgctcctg accctcatcc tgagtcattg ttcctgttag
    11941 cgtcctcgct ctatgggtgc tactttacgc ctttgcttta aacaaacaat caaccgccat
    12001 cagtatttgc acattttcag agttttatat aaaagcaatt cactgattta tatagcttat
    12061 ttgacatgca tcgcagaatt ttctctgcta tttcgcataa agactatcgg caatgtctca
    12121 caaagcgtat ggcttttact tacaacgctc acgccgaaaa atccgccttt tattatggtt
    12181 caggtataat cgccacaaag ctttgactga cggagacgac cgctttgact aatttgaatt
    12241 atcaacagac gcattttgtg atgagtgcgc ctgatattcg ccacctacct tccgataccg
    12301 gaattgaagt ggcttttgca ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc
    12361 tgactaacca gaaaagcctg gctcgtacct caaaaacccc agggcgcacc cagcttatca
    12421 acctgtttga agtggctgac ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg
    12481 aagtcccgga agagatgaag cgcaaatggc agcgtgcgct cggcgaatac ctcgaaaaac
    12541 gtcagagcct gcaaggtctg gtggtgctaa tggatattcg ccatccgctg aaagatttgg
    12601 atcagcagat gattgagtgg gcggtagaca gcaatatcgc cgttctggtg ctgctgacca
    12661 aagcggacaa actggcaagc ggcgcacgta aagcgcaatt gaatatggtg cgtgaagctg
    12721 tactggcgtt taacggtgat gtgcaggttg aaacgttttc ttcgttgaag aaacaaggcg
    12781 tggacaagct gcggcagaaa ctggatacct ggtttagcga gatgcagcct gtagaagaaa
    12841 cgcaggacgg cgaataattt tcttgcctta atgcttgtgc cggatgtggc gtatccggcc
    12901 cgtaaattca atagattaca attctaccgt tggcctgata aacatagcgc atcaggcatt
    12961 acggatcttt tctttcgccc aataaaaaac gccccagtca ttactgactg gggcggctaa
    13021 aatattcagc caaatccgat tacgtgaagt aaaaggtctg aaagatagaa catcttacct
    13081 ctgtacccta cgcgactaac tttactcttt tttgttcagt tcagaaagca ctttttgtaa
    13141 ttttttttca gttgttgcat aggaatttct aatagccatc acaaaacgtg acagcttatg
    13201 ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg atcccagttt
    13261 tcgccactcc ccacttccac cagcaacggc acatccagac gggtacagtt ttccatcagt
    13321 tgatgaatct gcttcgcgac ggcatcaaca tcatctttat gaacttcaaa taccagttca
    13381 tcgtgtacct gcatgatcat acgtacacgc ggttgctcag cctgtaacca cgcatcaacg
    13441 gcaatcatcg cccgtttgat aatgtcggcg gcggttccct gcattggcgc gttaatggct
    13501 gcacgttcag ccgctgcacg acgagcacca ttgctggatt tgatatccgg cagatacaga
    13561 cggcgtccgt ccagcgtttc aacgtagccc tgctctttcg cctgagcacg ggtgcgttcc
    13621 atatactcca gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct
    13681 ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac tcatgccata aatcagacca
    13741 aagttgatcg ctttcgcgct acggcgttgc tcgctggtga cggtttccag tggcaaacca
    13801 aacacttctg ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc ggtcagcaag
    13861 cctttgtcac gcgaaagatg cgccataatg cgcagttcaa tctgcgagta gtccgctgag
    13921 acaatcacat aatcctctgg cgcaataaac gcctggcgga tacgacgacc ttcttcgtta
    13981 cgcaccggaa tgttttgcag gttaggatcg gttgacgata aacgtcccgt tgcagttact
    14041 gcctggtgat aagaggtatg cacacgcccg gttttcgggt tgatcatcag cggcagcttg
    14101 tcggtgtagg tcgatttcag cttcgccaga ccacgatact ccagaatcac ttttggcaac
    14161 ggatagtcca gcgccagttc ttccagtacc tcttccgacg ttgacggcgc gccacccggc
    14221 gttttcttca gcggtttaat accctgtttt tcaaacagaa tggtttgcaa ctgcttggtg
    14281 gaagaaaggt taaattcttc acctgcaatt tcatgcgctt tcttttccag ctcagccaga
    14341 cgcaaggtga gctcttcaga atgattgtgc agcactttcg gatcgatctt cacaccgtta
    14401 cgttcaatgc gtgaaagcac cggcaccagc ggcatttcga tattctcgaa gacgttcaac
    14461 ggccctttgt gtttttgcag atctggccac attttcagat gcaactgcaa ggtgacatca
    14521 gcatcttcgg cggcgtaacg tccggcctct tcgagggcaa tctggttaaa ggtcagttga
    14581 tttttgcctt tacccgcaat ctcttcaaaa gtgatggttt tgtgcttcaa ccaacgttcc
    14641 gcgaggctgt ccatatcgtg acgcccggca acgctattga gaatgtagga ctccagcatg
    14701 gtatcaaacg caatcccacg cagctcaatg ccgtaattcg ccagaatacc gcgatcgtat
    14761 ttcaggtttt gcccgacctt cagcgccttt tcatcttcca gcagcggttt tagcaactcg
    14821 agtgcacgct cgcgagagat ttgatcgggc gcgtcaagat aatcatgagc aaccggaata
    14881 tatgccgcta cgcctggctc gatagcaaaa gaaagcccga ccaggttagc agagatgtta
    14941 tcaaggctgt cggtttcggt atcaaatgca aataccggcg ctttttccag cttcgcaatc
    15001 cacgctttca gtgtttcttc atcaaggatg gtgacgtagt tgtcataaga aatcaccgtt
    15061 gccgtcactt ctggcgcttc gtctgcaaca ctggtttcct gcggcttcgc ggctggtttt
    15121 gcccctttgg cctgtaacca tttgcccgct tcgacatcag cagtccagcg tttgaactca
    15181 tactttttga acagccccaa caactcttcc gctgccggtt gctgcacttc cagttgttca
    15241 caggtcagct ccagttcaac gtcggtttta atcgtcgcca gctggtatga gagataagca
    15301 acttctttgt tttgctcgag cttcgctgcc attgttttcg cgccacggaa gctcaaccca
    15361 gcaatttttt ctggctcggc atacagcgta tccagcccgc ccagaccttg cagcaatgcc
    15421 tgcgcggttt tttcaccgac gcccggtacg ccagggatgt tatcggagga gtcacccatc
    15481 agcgccagga aatcgatgat cagttctggc ggcacgccgt acttattcac cacctcttcc
    15541 ggtccgagga tggtattcgt catggtattg ataagcgtaa tatttggcgt caccagctgc
    15601 gccatatctt tatcgccagt gctgatcagc accggacgcc cggctttttc ggcttcgcgc
    15661 gccagagtac cgataacgtc gtccgcttct acgccagaaa ccgccagcag cggcagtccc
    15721 atcgctttaa ccatcgcgtg cagaggttcg atttgtgctc gcagatcgtc cggcattggc
    15781 gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca
    15841 aagaccaccg ctgcatgcgt cggtttatat tgcatgatca gactgcgcag catgttgagg
    15901 acaccataca tcgcaccggt cggctcgcct gcgctgttag tcagcggggg aaacgcgtga
    15961 tatgcgcgat aaagataaga tgaaccatct acaaggataa gtggattttg ggggatctga
    16021 accataatgt ccgtgcctgt ttatcagatt atgggtaaag gatgccacag acaggataaa
    16081 aacatgagtt tttcaccgct tttcgtgaaa atcttttgta gatcttctgg atcgctcgca
    16141 aatcgtcatg tggataactt tgtgcataaa ttatttac
//
LOCUS       DAJSKJ010000081        16133 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000081 DAJSKJ010000000
VERSION     DAJSKJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.081"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..399
                     /locus_tag="ON345_002263"
     CDS             <1..399
                     /locus_tag="ON345_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="in Salmonella is responsible for long O-antigen
                     polymerization; may be part of the ferric enterobactin
                     transport system; necessary for enetrobactin uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR3072319.1"
                     /translation="IANAAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLEIEKAVT
                     DVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKDGPGKAIIVILSAL
                     IGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(396..1211)
                     /gene="fepC"
                     /locus_tag="ON345_002264"
     CDS             complement(396..1211)
                     /gene="fepC"
                     /locus_tag="ON345_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR3072320.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(1208..2200)
                     /gene="fepG"
                     /locus_tag="ON345_002265"
     CDS             complement(1208..2200)
                     /gene="fepG"
                     /locus_tag="ON345_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR3072321.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLDTSQVFATLM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILILIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(2197..3201)
                     /gene="fepD"
                     /locus_tag="ON345_002266"
     CDS             complement(2197..3201)
                     /gene="fepD"
                     /locus_tag="ON345_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR3072322.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSVDCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            3312..4562
                     /gene="entS"
                     /locus_tag="ON345_002267"
     CDS             3312..4562
                     /gene="entS"
                     /locus_tag="ON345_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR3072323.1"
                     /translation="MNKQSWLLNLSLLRTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMMGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAVIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            complement(4566..5522)
                     /gene="fepB"
                     /locus_tag="ON345_002268"
     CDS             complement(4566..5522)
                     /gene="fepB"
                     /locus_tag="ON345_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3072324.1"
                     /translation="MRLAPLYRNALLVTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            5897..7072
                     /gene="entC"
                     /locus_tag="ON345_002269"
     CDS             5897..7072
                     /gene="entC"
                     /locus_tag="ON345_002269"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR3072325.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRNQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            7082..8692
                     /gene="entE"
                     /locus_tag="ON345_002270"
     CDS             7082..8692
                     /gene="entE"
                     /locus_tag="ON345_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR3072326.1"
                     /translation="MSIPFTRWPEEFARCYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            8706..9563
                     /gene="entB"
                     /locus_tag="ON345_002271"
     CDS             8706..9563
                     /gene="entB"
                     /locus_tag="ON345_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR3072327.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            9563..10309
                     /gene="entA"
                     /locus_tag="ON345_002272"
     CDS             9563..10309
                     /gene="entA"
                     /locus_tag="ON345_002272"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR3072328.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            10312..10725
                     /gene="entH"
                     /locus_tag="ON345_002273"
     CDS             10312..10725
                     /gene="entH"
                     /locus_tag="ON345_002273"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR3072329.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            10906..13011
                     /gene="cstA"
                     /locus_tag="ON345_002274"
     CDS             10906..13011
                     /gene="cstA"
                     /locus_tag="ON345_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR3072330.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            13193..13390
                     /locus_tag="ON345_002275"
     CDS             13193..13390
                     /locus_tag="ON345_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR3072331.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(13400..14488)
                     /gene="hcxA"
                     /locus_tag="ON345_002276"
     CDS             complement(13400..14488)
                     /gene="hcxA"
                     /locus_tag="ON345_002276"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR3072332.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            14597..15757
                     /gene="ybdL"
                     /locus_tag="ON345_002277"
     CDS             14597..15757
                     /gene="ybdL"
                     /locus_tag="ON345_002277"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR3072333.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(15758..>16133)
                     /locus_tag="ON345_002278"
     CDS             complement(15758..>16133)
                     /locus_tag="ON345_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR3072334.1"
                     /translation="TQPIVVTHTDKNALEIVDGFHRHEIGKGSSSLKLRLKGYLPVTC
                     LEGTRNQRIAATIRHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRL
                     KQINGLQELFADRQYSRAWTVK"
BASE COUNT         3502 a         4275 c         4677 g         3679 t
ORIGIN      
        1 attgccaacg cggcgggaat taaaaagccc gtatacagta atggtcaggc cgttaaagat
       61 gaccccgatt tctctatttc tctcggcgca gacggtattg aacgcaaact ggaaattgaa
      121 aaagcggtca ctgacgttgc ggaactgaac ggtgaattac gtaatcggca gtatcttgtc
      181 gagcaattaa caaaagcaaa tgtcaacgat gtgaatttta cgccatttaa atatcagtta
      241 agcccgtcat tgccagtgaa aaaagacggt ccgggtaagg cgattattgt gatcctttcc
      301 gcgctgatcg gcgggatggt ggcttgtggt agcgtgttat tgcgctatgc gatggcatcc
      361 agaaaacagg atgccatgat ggcagaccac ttagtttaac tatttgcggt tgaaggtgcc
      421 gttcgtccga gcggcaccac aagcggcgtt ccggcaactg gatcgtcaat aatcatgcag
      481 cgcagaccat aaatgcgctc aatcaattca gcagtgacaa tctcctttgg cgctccctga
      541 gcaacaattt tcccttcccg caacgcaatc aaatggctgg cgtaacgaca ggcctgatta
      601 agatcgtgca acaccgccgc cagggtatag cctttctcgc ggttcagttc gcttaacagt
      661 tccagtagat caatctgatg actgatatcc agccaggtcg tgggttcgtc gagcagcata
      721 attgccgttt cctgggccag caccatcgcg atccacgctc gctggcgttg tccgccagaa
      781 agggtatcca cgctttgatc tgccagatga gttattcccg tggcctgcat cgctttcgtt
      841 accgcttctt catcctcttt gcgccaacgg gtaaacagcg gttgatgcgg gtaacgtcca
      901 cgcgccacca gctcctgcac ggtgatatcg cccggcgtgg tagcattttg cgccaacaga
      961 ccaatccggc gtgcaacttc tttactggcg taatgttgaa tgtgctcgcc atccagccag
     1021 acatgcccgt gagcaggcgt catcaggcgg cttaaggtac gcagcaacgt ggatttaccg
     1081 cagccatttg gcccgataat tgccgtgaag tgaccatcag gtatttctac agtcagattt
     1141 tccgcaacgg tatatttgcc atatccgagg gttaactgtt cgccacgcaa acgggctact
     1201 gattcggtca ttttttgcga gactcctgaa ttaacaagac gataaggtaa ataccgccga
     1261 ggctgacggt aacgacgcca accggaagtt gatacggcat aaacagttgt tgggcgcaga
     1321 gatcggccgc cagcagtaac agcgctccgc acagcgccgc ctgggtcagc ccccagcgag
     1381 cggtgccgct aatgcgtcgg gcaatgtgcg gcgcgactaa agcaataaaa gaaatcggcc
     1441 cggcaagcgc cgttgccgca gcggtaagca ccactgcaac cagcatcatt aacagacgcg
     1501 aacgttcgac gctgacgccc agcgcgcagg cggtatcatc gcccatctcc agcaagcgca
     1561 ttcgccgcac cagcaaggcg gcggcaatga ggatcaatat aatgatgggt gcggaaggcg
     1621 aagtttttgc ccacgtcagg ccattgagcg atccggcatt ccacaatcct gcggttagcg
     1681 ccgtttctaa agacgctttc agcaacagcc aggtattaaa ggccaccagc atggcgcgaa
     1741 cgccgatacc gataataatc aaccgaaagg tgtcgatgcc gttacgccag gcgagcagcc
     1801 agaccaacag cgaagtgaca atgccgccca ccattgctgc cagcgcgata gccgtcaggt
     1861 cctgaccaaa cagcaccatc gccaccagca cgccgctcca cgccccggtg ttaaagccca
     1921 ttacgtcagg gctgccgagc gggttacgca tcagcgactg aaaaatcgca ccgctgacgc
     1981 ccagtgccgc gccaatcaac agcgccatca gcacccgtgg taaacgccat tcggtgacca
     2041 ccatcgtcat actgcgcggc gcatcgccca tcagtgtggc gaatacctgc gaggtgtcca
     2101 gcgtgacggc accgctgcgt aatccccaga tacctgcaac cacacaggcg gaaatcagca
     2161 gcaaacaggt gatgagtaat cggcgagaga cgtaaatcat gcgccacctc gcgttttacg
     2221 tcgcacgagg aagattagca ccggtgcacc aataaacgca ctgaccacag aaacgcgcaa
     2281 ctcgccgggc acaatcaccc gcccgatgat atcggcaaac agcagcaggg caggggtggc
     2341 gagtagcgtg acgggcagcg accagcgatg atcggcaccc accagccaac gcgccatatg
     2401 cggcatcatc aggccaataa aggcaatcgg gccaactacc gctgtcgcac taccacaaag
     2461 cacggtaatc gccagcagac caattaactg tgtgcgcgcc acgcgactgc ccagcgccgt
     2521 cgcggtgtcg ctgccgaggc ttaaactgtt cagcgcgcgg ctcagtaata gcgcggttgc
     2581 tccggcgatc agcaccggga tcagcaccac ttttaaggta tgtagattgc gaatatccag
     2641 cgaaccggct tgccagaaac gcagttgatc gtagacgtca ggattaagca gggcgatgcc
     2701 gctggtcagc ccttccagca ccgccgccag cgccacgccc gccagggtta aacgcaccgg
     2761 acttaactgc ccgccgccct ggctgccggt aaaggcaaca atcaacgagg ccaccagcgc
     2821 cccggcgaag gccatcgcca gttgttcctg cgcggatgag taaccaaaca gcgccgcacc
     2881 cagcacaatg gcaaagctgg ctccggcgtt cacgccaagc aagccggggt cggcaagtgg
     2941 atttcgggtg agggtttgca tcaacgcccc ggcaaggcca agcgcgccgc ctgccagtaa
     3001 accggcaagg gtgcgcggca gtcgcgcgtc gagcacgatg gtgcagtcga cgctctggca
     3061 ggtgccggag agggcctcca gcactacgga agcggggagt gattttgccc caatgagcag
     3121 gcttaatgcc gtcgcgataa tcagtaataa cagcaatccg ggcacggcaa tggcgcgtgt
     3181 cacggcaaca gaaccagaca tacgaacttc catgataatg aaattaatta tcgttatcga
     3241 tcttatttgg atatgttagc atgtgcagcc taagaatagg tatttaaaat atttgatggc
     3301 aaggcattgt aatgaataaa caatcctggc tgcttaacct cagcctgttg agaacgcacc
     3361 cggcgtttcg cgcagtattc ctcgctcgtt ttatctcaat tgtgtctctg ggtttgctcg
     3421 gcgtcgcggt accggtgcag atccagatga tgacgcattc tacctggcag gtggggcttt
     3481 cggtgacgct gaccggcggc gcgatgtttg ttggcctgat ggttggcggt gtgctggcgg
     3541 atcgctatga gcgcaaaaaa gtgattttgc tggcgcgcgg cacctgtggc attggcttca
     3601 ttggactgtg cctgaacgcg ctgctgccgg agccttcatt gctggcaatc tatttacttg
     3661 gtttatggga tggttttttc gcatcgcttg gcgttacggc gctactggcg gcgacacctg
     3721 cactggtagg gcgtgaaaac ttaatgcagg ccggggcgat caccatgttg accgtgcgtc
     3781 tgggatcggt gatttcgcct atgatgggcg gtttattgct ggcgaccggc ggcgtagcct
     3841 ggaactacgg gctggcggcg gcgggcacgt ttattacctt gttaccgttg ttaagccttc
     3901 cggcgttgcc accgccaccg cagccgcgtg agcatccgtt gaaatcatta ctggcaggat
     3961 ttcgttttct gctcgccagc ccgctggtag gagggattgc gctgctgggt ggtttattga
     4021 cgatggcgag cgcggtgcgg gtactgtatc cggcgctggc tgacaactgg cagatgtcgg
     4081 cggcacagat tggttttctc tatgcggcga tcccgctcgg cgcggttatt ggcgcgttaa
     4141 ccagcgggaa gctggcacat agcgcgcgac cagggttatt gatgctgctc tccacgctgg
     4201 gatcgttcct cgccattggt ctgtttggcc tgatgccgat gtggatttta ggcgtggttt
     4261 gtctggcgct gttcggctgg ttgagtgcgg tcagctcgtt gctgcaatac acaatgctgc
     4321 aaacgcaaac cccggaagcg atgttagggc ggattaacgg tttgtggacg gcgcaaaacg
     4381 tgacgggcga tgccataggt gcggcgctgt taggcggtct gggggcgatg atgacaccgg
     4441 ttgcttccgc aagcgcgagc ggttttggat tgttgattat cggtgtgttg ttattgctgg
     4501 tgctggtgga gttgcgacgt tttcgccaga cgccgccgca ggtgacagcg tccgacagtt
     4561 aatgcttaaa acagcgcctt aagtcgttcc agcacttgca tggcgctgta gtaatccagg
     4621 cggaacgtct cggttcccag cgcataaacc tgcttgtttt gtactgcggg caggtgcgcg
     4681 agcagtggat tggcataaat cgcatcggcg tctttctgat caccggcgaa caggaatagt
     4741 gactcgccat ttaaccctgc agccagattt tctccaccaa gctgaatgat gtcatggcgt
     4801 ttgccctggc tttggctggc gtttaaccct gcgggcaact tcgccagcgt aaagccgagt
     4861 tgttccagca tctgcccttg tgctgattcc ggcgtccaga gattggcact gtgtgcagcg
     4921 gcggtataga caatggcagt gaccggctgc ggcggtaatt tgatttgctc tttcgccgct
     4981 gccagttgct tatcaaactg tgcaatccgc tctgccgctt gtttctcatg cccggtaatt
     5041 tcgccaagct gcgttaacag cgactgccag cttttgtcgt cgtaattgat gattaatgtc
     5101 ggggcgatgg tggaaagctg atcatacagt gccagcgccg aatccccgcc ggttgcgcta
     5161 attaaaatca gatccggcat ttgcgcggca acggcttcgg cgctcggttc gccgatatag
     5221 agccgttgca gtttgcgttc tttcgccacc ttgctccact ggcgtaaaaa gccctggtca
     5281 tccgcgacgc ggttattcgg cgtggtcgcg ccgctggcga tcaccggagc atcaatcgcc
     5341 agcagtgagc cggtcagggt gacgctggtg gaaacaatac gctgcggctg gctttccagt
     5401 gtatgtgtgc cacggctgtc agtaatctga cgcggccagt cagcggcctg aactgcggct
     5461 attcctgaaa gcaaaagccc tgttactaga agggcgttgc ggtagagcgg ggcgagtctc
     5521 acaaatcagc ttcctgttat taataaggtt aagggcgtaa tgacaaattc gacaaagcgc
     5581 acaatccgtc ccctcgcccc tttggggaga gggttagggt gaggggaaca gccagcactg
     5641 gtgcgaacat taaccctcac cccagcccta accctggaag ggagaggggg cagaacggcg
     5701 caggacatca cattgcgctt atgcgaatcc atcaataatg cttctcattt tcattgtaac
     5761 cacaaccaga tgcaaccccg agttgcagat tgcgttacct caagagttga catagtgcgc
     5821 gtttgctttt aggttagcga ccgaaaatat aaatgataat cattattaaa tcctttatca
     5881 ttttgtggag gatgatatgg atacgtcact ggctgaggaa gtacagcaga ccatggcaac
     5941 acttgcgccc aatcgctttt tctttatgtc gccgtaccgc agttttacga cgtcaggatg
     6001 tttcgcccgc ttcgatgaac cggctgtgaa cggggattcg cccgacagtc ccttccagca
     6061 aaaactcgcc gcgctgtttg ccgatgccaa agcgcagggc atcaaaaatc cggtgatggt
     6121 cggggcgatt cccttcgatc cacgtcagcc ttcgtcgctg tatattcccg aatcctggca
     6181 gtcgttctcc cgccaggaaa aacagacctc agcccgccgt tttacccgca accagtcgct
     6241 gaacgtggtg gaacgccagg caattcctga acaaaccacg tttgaacaga tggttgcccg
     6301 cgccgccgca cttaccgcca cgccgcaggt cgacaaagtg gtgttgtcac gattgattga
     6361 tatcaccact gacgccgcca ttgatagtgg cgtattgctg gaacggttga ttgcgcaaaa
     6421 cccggttagt tacaacttcc atgtcccgct ggctgatggt ggcgtcctgc tgggggccag
     6481 cccggaactg ctgctacgta aagacggcga gcgttttagc tccattccgt tagccggttc
     6541 cgcgcgtcgt cagccggatg aagtgctcga tcgcgaagcg ggtaatcgtc tgctggcgtc
     6601 agaaaaagat cgccatgaac atgaactggt gactcaggcg atgaaagagg tactgcgcga
     6661 acgcagtagt gagttacacg ttccctcctc tccacaattg attaccacgc cgacgctgtg
     6721 gcatctcgca actccctttg aaggtaaagc gaattcgcaa gaaaacgcac tgactctggc
     6781 ctgtctgctg catccaaccc ccgcgctgag cggtttcccg catcaggccg cgacccaggt
     6841 tattgctgaa ctggagccat tcgaccgcga actgtttggc ggcattgtgg gttggtgtga
     6901 cagcgaaggt aacggcgaat gggtggtgac catccgctgc gcgaagctgc gggaaaatca
     6961 ggtgcgtctg tttgccggag cggggattgt gcctgcgtcg tcaccgttgg gtgagtggcg
     7021 cgaaacaggc gtcaaacttt ctaccatgtt gaacgttttt ggattgcatt aaggagcgag
     7081 gatgagcatt ccattcaccc gctggccgga agagtttgcc cgttgctatc gggaaaaagg
     7141 ctactggcag gatttgccgc tgaccgacat tctgactcgc cacgctgcga gtgacagcat
     7201 cgcggttatc gacggcgagc gacagttgag ttaccgggag ctgaatcagg cggcggataa
     7261 cctcgcgtgt agtttacgcc gtcagggcat taaacctggt gaaaccgcgc tggtacaact
     7321 gggtaacgtc gctgaattgt atattacctt tttcgcgctg ctgaaactgg gcgttgcgcc
     7381 ggtgctggcg ctgttcagcc atcagcgtag tgaactgaac gcctatgcca gccagattga
     7441 acccgcattg ctgattgccg atcgccaaca tgcgctgttt agcggggatg atttcctcaa
     7501 tactttcgtc acagaacatt cctccattcg cgtggtgcaa ctgcacaacg acagcggtga
     7561 gcataacttg caggatgcga ttaaccatcc ggctgaggat tttactgcca cgccatcacc
     7621 tgctgatgaa gtggcctatt tccagctttc cggcggcacc accggcacac cgaaactgat
     7681 cccgcgcact cataacgact actactacag cgtgcgtcgt agcgtcgaga tttgtcagtt
     7741 cacacaacag acacgctacc tgtgcgcgat cccggcggct cataactacg ccatgagttc
     7801 gccgggatcg ctgggcgtct ttcttgccgg aggaacggtt gttctggcgg ccgatcccag
     7861 cgccacgctt tgcttcccat tgattgaaaa acatcaggtg aacgtcaccg cgctggtgcc
     7921 accggcagtc agcctgtggt tgcaggcgct gaccgaaggt gaaagccggg cgcagcttgc
     7981 ctcgctgaaa ctgttacagg tcggcggcgc acgtctttct gccacccttg cggcgcgtat
     8041 tcccgctgag attggctgcc agttgcagca ggtgtttggc atggcggaag ggctggtgaa
     8101 ctacacccgt cttgatgata gcgcggagaa aattatccat acccagggtt acccaatgtg
     8161 cccggacgat gaagtgtggg ttgccgatgc cgaaggaaat ccactgccgc aaggggaagt
     8221 tggacgcctg atgacgcgcg ggccgtacac cttccgcggt tattacaaaa gtccgcagca
     8281 caatgccagc gcctttgatg ccaacggttt ttactgttcc ggcgatctga tctctattga
     8341 tccagagggt tacatcaccg tgcaggggcg cgagaaagat cagattaacc gtggcggcga
     8401 gaagatcgct gccgaagaga tcgaaaatct gctgctgcgc catccggcgg tgatctacgc
     8461 cgcactggtg agcatggaag atgagctgat gggcgaaaaa agctgcgctt atctggtggt
     8521 gaaagaaccg ctgcgcgcgg tgcaggtgcg tcgtttcctg cgtgaacagg gtattgccga
     8581 atttaaatta ccggatcgcg tggagtgtgt agattcactt ccgctgacgg cggtcgggaa
     8641 agtcgataaa aaacaattac gtcagtggct ggcgtcacgc gcatcagcct gaaggagaga
     8701 acacgatggc tattccaaaa ttacaggctt acgcactgcc ggagtctcac gatatcccta
     8761 agaataaagt cgactgggcc tttgaaccgc aacgtgccgc gttgttaatc catgatatgc
     8821 aggactattt tgtcagcttc tggggcgaga actgcccgat gatggagcag gtaatcgcga
     8881 atattgctgc gctgcgcgac tactgcaaac agcacaatat cccggtttat tacaccgccc
     8941 agccgaaaga gcagagcgat gaagatcggg cgctgttgaa tgatatgtgg gggccgggcc
     9001 tgacccgctc gccggaacag caaaaggtgg tggatcgcct gacgccagat gccgacgaca
     9061 cggtgctggt gaagtggcgc tacagcgcgt ttcatcgttc tccgctggag caaatgctca
     9121 aagagagtgg acgtaaccaa ctgattatta ccggggtata tgcccacatt ggctgtatga
     9181 ccaccgcaac cgacgcattt atgcgcgata ttaaaccgtt tatggtggcg gatgcgctgg
     9241 ccgatttcag ccgtgacgag catttgatgt cgctgaaata tgtggccgga cgttctggcc
     9301 gggtggtgat gaccgaagaa ttactgccag cacctgttcc cgccagtaaa gcggcgctgc
     9361 gtgaggtaat cctgccgttg ctggacgagt ccgatgaacc gttcgatgac gacaacctga
     9421 tcgactacgg tctggattcg gtgcgcatga tggcgctggc ggcgcgctgg cgcaaagtgc
     9481 atggcgatat cgattttgtc atgctggcga aaaacccgac catcgacgcc tggtggaagc
     9541 tactctcccg cgaggtgaaa taatggattt cagcggtaaa aatgtttggg taaccggcgc
     9601 ggggaaaggt atcggctacg ccacggcgct ggcatttgtt gaggctggag cgaaagttac
     9661 aggttttgat caagcgttca ctcaggagca atatcccttt gcgaccgaag tgatggatgt
     9721 tgccgatgct gcgcaggtgg cgcaggtatg tcagcgactg ttagctgaaa cggagcgact
     9781 ggacgtgctg gtcaatgcgg cgggaatttt gcgcatgggc gcgacagacc aactgagcaa
     9841 agaggactgg cagcagactt ttgcggttaa cgtcggcggt gcgtttaacc tgtttcagca
     9901 aaccatgaac cagtttcgcc gtcagcgggg cggggcgatt gtcactgtgg cgtccgacgc
     9961 cgcacacacg ccgcgtattg gtatgagtgc ttatggcgca tcgaaagcgg cgctgaaaag
    10021 cctggcgttg agcgtcgggc tggaactggc gggtagcggc gtgcgctgta atgtggtttc
    10081 gcctggctcc accgacaccg atatgcaacg cacgctgtgg gtgagcgatg acgccgaaga
    10141 acagcgtatt cgcggctttg gcgagcagtt taaactcggc attccgctgg ggaaaatcgc
    10201 ccgtccacaa gagatcgcca acacgatttt gttcctcgcc tctgacctcg ccagccatat
    10261 caccctacag gatattgtgg tcgatggcgg ctcaacgctg ggggcataag catgatctgg
    10321 aaacgccatt taacgctcga cgaactgaac gccaccagcg ataacacaat ggtggcgcat
    10381 ctgggaattg tgtatacccg tctgggcgat gatgtgctgg aagccgaaat gccggttgat
    10441 acccgtactc atcagccgtt tggcctgctg catggcggcg cgtcggcggc gctggctgaa
    10501 acgctgggat cgatggccgg atttatgatg acccgtgacg ggcagtgtgt ggtgggcacg
    10561 gaacttaacg ccacccatca tcgccctgtg tctgaaggca aggtacgcgg cgtctgccag
    10621 ccgctgcatc ttgggcggca aaatcagagc tgggaaatcg tcgttttcga tgaacagggg
    10681 cggcgttgct gcacttgtcg gctgggtacg gcagttttgg gatgaacccg acagaattat
    10741 atgagattgc aggaaaactc ggttaacgga gtgatcgagt taacattgtt aagttaaata
    10801 ttggtttcaa ctccgattta catggttgcc gtgttgttaa atcgtacaaa gatgttatag
    10861 aaacaaaatg taacatctct atggacacgc acacggataa caactatgaa caaatcaggg
    10921 aaatacctcg tctggacagt gctctctgta atgggagcat ttgctctggg atacattgct
    10981 ttaaatcgtg gggaacagat caacgcgctg tggattgtgg tggcgtcggt ctgtatctat
    11041 ctgatcgctt accgttttta tgggctgtat atcgccaaaa atgtgctggc ggttgacccg
    11101 acgcgtatga cgccagcggt gcgccataac gacgggctgg actatgtgcc gacggacaag
    11161 aaagtgctgt tcggtcacca ttttgcggcc attgccggag cagggccgct ggtggggccg
    11221 gtgctggcag cgcaaatggg ctacctgccg gggatgatct ggctgctcgc cggggtggtt
    11281 ctcgccggtg cggtgcagga tttcatggtg ctgtttgttt ctacgcgccg tgacggtcgc
    11341 tcgctgggtg agctggtcaa agaagagatg gggccaactg ccggggtgat tgcgctggta
    11401 gcctgcttta tgatcatggt cattatcctt gcagtactgg cgatgatcgt ggtgaaagcc
    11461 ctgactcata gcccgtgggg aacgtatacc gttgcgttca ccattccgct ggcgattttt
    11521 atgggaatct acctgcgcta tctgcgtccg ggccgcattg gcgaagtgtc agtgattggc
    11581 ctggtgttcc tgattttcgc cattatctct ggcggctggg tggcagaaag tccgacctgg
    11641 gcaccgtact ttgactttac cggcgtgcag ctgacctgga tgctggtggg ttacggtttt
    11701 gtggcggcgg tgctgccggt gtggttgctg ctggccccgc gtgactacct ctctaccttc
    11761 ctgaaaatcg ggactatcgt cggtctggcg gtaggcattt tgattatgcg tccgacgctg
    11821 accatgcctg cgctgaccaa atttgtcgat ggcactggcc cggtatggac cggtaatctg
    11881 ttcccgttcc tgtttatcac catcgcctgt ggcgcggtgt caggcttcca tgcgctgatc
    11941 tcttccggca ccacgccgaa gatgctggcg aacgaagggc aggcgtgctt tatcggctac
    12001 ggtggaatgt taatggaatc cttcgtggcg attatggcgc tggtttccgc ctgtatcatc
    12061 gatccgggcg tttactttgc catgaacagc ccaatggcgg tgctggctcc ggcagggacg
    12121 gcggatgtgg tcgcttctgc cgcgcaggtg gtgagtagct ggggctttag cattacacca
    12181 gatacgctca accagattgc cagcgaagtg ggtgaacagt cgatcatttc ccgtgcgggc
    12241 ggtgcgccga cgttggcggt ggggatggct tacatcctgc acggtgcgct gggtggtatg
    12301 atggatgtag cgttctggta tcacttcgcc attctgtttg aagctctgtt tattctgacg
    12361 gcggtagatg cgggtacgcg tgctgcgcgc tttatgttgc aggatctgct gggcgtggtg
    12421 tctcctggcc tgaagcggac cgattcactg cctgctaacc tgctggcaac ggcgctgtgc
    12481 gtactggcgt ggggctactt cctccatcag ggcgtggtcg atccgctggg cggtattaac
    12541 actctgtggc cgctgtttgg tattgccaac cagatgctgg cagggatggc actgatgctc
    12601 tgtgccgtgg tgttgttcaa gatgaaacgt caacgttacg cctgggtggc gctggtacca
    12661 acggcctggc tgctgatttg taccctgacc gcaggctggc agaaagcgtt tagcccggat
    12721 gcgaaagtcg gcttcctggc cattgctaat aagttccagg caatgatcga cagcggcaat
    12781 attccgtcgc agtatactga gtcacagctg gcgcaactgg tgttcaataa ccgtctggat
    12841 gccgggttaa ccatcttctt tatggtggtc gtggtggttc tggcactgtt ctcgattaag
    12901 acggcacttg cggcattgaa agatccgaag ccaacggcga aagaaacgcc gtatgagccg
    12961 atgccggaaa atgttgagga gatcgtagcg caggcgaaag gcgcgcacta aagtcagagt
    13021 gaggggggat gttggcgaat gttggcatag tgccgagggt tccccctcac cctaaccctc
    13081 tccccgatgg ggcgagggga ctgaccgagt gcgttgatag catttgtagg ccggataaag
    13141 cgttcacgcc gcatccggca ctctttcagc aacatggtta gcggaggcca agatgtttga
    13201 ttcactggca aaagccggaa aatatttagg tcaggcggcg aagctgatga ttggtatgcc
    13261 tgattacgac aactatgtcg aacatatgcg ggttaaccat cccgatcaaa cgccgatgac
    13321 ctacgaggag tttttccgtg agcggcagga cgcgcgctac ggtggaaaag gcggcgcgcg
    13381 ctgctgctaa atttgcacgt caggctttaa acgattccac tttttcgaac gctgcacgca
    13441 acgtatctgg tgtcagcgtg actggcaggt aatgaatgga ctccaccggg cgcaaggtgt
    13501 gggcaatcac tttgtcgatc tccgcctgat tattgatatc cacttccagc tccgccagcg
    13561 tagtcggcag atgaaaacgc tgatacgccc cggttaattg cgccagcaca tcatcctgac
    13621 ccagcaaggc gctttgcacc agaattccgt aagcgacttt ggtgccgtga agaaacttct
    13681 cggtttgcgg caggacagtc agaccgttat gcacggcatg agccgccgcc acacgcgtaa
    13741 aacgatcgcc cagaccaccg accatcccac caccagcaat aatagcatcc accacatcgc
    13801 aaaatgattg cgttaactgc tgattttgct gatcggcaag tgcctgttcg ctactgttta
    13861 acaagacgtc gcgaatggcc tgcgcattat tgatccccag ccgcacggtt agcggcaacg
    13921 tttctggttg cggagccagc accaccgctt cataccattt cgccagcgtg tcaccgatcc
    13981 ccgccagcag atattgttgc ggtgcattga ggataatctc cggttccacc agcaccataa
    14041 aattggcgtc gtcgaaaatc tcataatgca gcgcctgtcc ggcatcgtta taccagacgg
    14101 agagcggtgt ccaggctgcg caggtggcgg cgatcgtcgg agcggcaaca aatggcagac
    14161 cgagacggcg ggcgagggct ttcgcggtgt cgagcagtgc gccgccgccg acgccaatca
    14221 ccacgctgcg atcgtcaccg gactcagccg ccagttgctg tacatcgctt tcgctgcaat
    14281 gaccacgaaa caaaatatgc tttgcccctg gaagtccaaa cgctggcgga agtttggttt
    14341 gcgccgcagc aatggcgcgt ttgccgtaga tccacaccgc gcgagaaagt tgttcatcag
    14401 tgaaaaaatc gtgcaggtga ttgaaacttc ctggatgtga aaagtagtta gccgggccga
    14461 cgaccacgcg gataggattg tgaggcatgt tgtgttgtcc ttattatttt atttagacat
    14521 ctaaacgtct tgattgccaa atactagcat cgtgttatag tgccttcaac acgcaacttc
    14581 gtcaggtaca ataaaaatga caaataaccc tctgattcca caaagcaaac ttccacaact
    14641 tggcaccact attttcaccc agatgagcgc gctggcgcag caacaccagg cgattaacct
    14701 gtcgcaaggc tttcctgatt ttgatggtcc gcgctattta caggagcggc tggcgtacca
    14761 cgttgcccag ggggcaaacc aatacgcgcc catgaccggc gtgcaggcct tgcgcgaggc
    14821 gattgctcag aaaacggaac gtttgtatgg ctatcaacca gatgccgata gcgatatcac
    14881 cgtaacggca ggggcgacgg aagcgttata cgcggcgatt accgcactgg tgcgcaatgg
    14941 cgatgaagtg atttgttttg atcccagcta tgacagttac tcccccgcca tcgcgctttc
    15001 tgggggaata gtgaagcgta tggcactgca accaccgcat tttcgcgttg actggcagga
    15061 atttgccgca ttattaagcg agcgcaccag actggtgatc ctcaacactc cgcataaccc
    15121 cagtgcaact atctggcagc aggctgattt cgccgctttg tggcaggcga tcgccgggca
    15181 cgagattttt gtcattagcg atgaagtcta cgagcacatc aacttttcac aacagggcca
    15241 tgccagtgtg ctggcgcatc cgcagctgcg tgagcgggca gtggcggttt cttcatttgg
    15301 caagacctat catatgaccg gctggaaagt gggttattgt gttgcgccag cgcccatcag
    15361 cgccgaaatt cgcaaggtac atcagtatct gaccttttcg gtgaataccc cggcacagct
    15421 ggcgcttgct gatatgctac gtgcagaacc tgagcattat cttgcgttac cggactttta
    15481 tcgccagaag cgcgatattc tggtgaatgc tttaaatgaa agccggctgg agattttacc
    15541 gtgtgaaggt acatactttt tgctggtgga ttacagcgcg gtttctaccc tggatgatgt
    15601 tgagttttgc cagtggctga cgcaggagca cggcgtagcg gcgattccgc tgtcggtgtt
    15661 ttgcgccgat cccttcccac ataaactgat tcgtctctgt tttgccaaga aggaatcgac
    15721 gttgctggca gcagctgaac gcctgcgcca gctttagcta tttaaccgtc caggcgcggg
    15781 aatattgacg gtcggcaaac aactcttgca ggccgttaat ttgcttcagg cgcagcactt
    15841 cgtcgctgtc cattcccagc tctttgccaa ttttattatc gtcccatccc aactggctaa
    15901 gttcacggac gatttccgac atggcggtga tttgatggcg tccacgagcg cgattatgac
    15961 gaatagtcgc ggcgatgcgt tgattacgcg taccttccag acaggtcacc ggcaaataac
    16021 cttttaagcg taattttaat gatgatgagc ctttaccaat ttcatggcgg tgaaaaccat
    16081 ccacgatttc cagggcattt ttatccgtat gcgtgaccac aatgggttgg gta
//
LOCUS       DAJSKJ010000082        15906 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000082 DAJSKJ010000000
VERSION     DAJSKJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.082"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1304
                     /gene="gntU"
                     /locus_tag="ON345_002279"
     CDS             <1..1304
                     /gene="gntU"
                     /locus_tag="ON345_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR3072335.1"
                     /translation="VLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKG
                     MGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAHYAIGLAGLVCALP
                     LFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPGPAPMLLASQMNA
                     DFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLS
                     LILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRQGMPK
                     DKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIAITCFVL
                     AAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGF
                     WLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(1361..1954)
                     /gene="yhgN"
                     /locus_tag="ON345_002280"
     CDS             complement(1361..1954)
                     /gene="yhgN"
                     /locus_tag="ON345_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR3072336.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2146..3249
                     /gene="asd"
                     /locus_tag="ON345_002281"
     CDS             2146..3249
                     /gene="asd"
                     /locus_tag="ON345_002281"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3072337.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3522..5708
                     /gene="glgB"
                     /locus_tag="ON345_002282"
     CDS             3522..5708
                     /gene="glgB"
                     /locus_tag="ON345_002282"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR3072338.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5705..7678
                     /gene="glgX"
                     /locus_tag="ON345_002283"
     CDS             5705..7678
                     /gene="glgX"
                     /locus_tag="ON345_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR3072339.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLKRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRCAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7696..8991
                     /gene="glgC"
                     /locus_tag="ON345_002284"
     CDS             7696..8991
                     /gene="glgC"
                     /locus_tag="ON345_002284"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR3072340.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            8991..10424
                     /gene="glgA"
                     /locus_tag="ON345_002285"
     CDS             8991..10424
                     /gene="glgA"
                     /locus_tag="ON345_002285"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR3072341.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10443..12890
                     /gene="glgP"
                     /locus_tag="ON345_002286"
     CDS             10443..12890
                     /gene="glgP"
                     /locus_tag="ON345_002286"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR3072342.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            13016..14523
                     /locus_tag="ON345_002287"
                     /pseudo
     CDS             13016..14523
                     /locus_tag="ON345_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
     gene            14539..15249
                     /locus_tag="ON345_002288"
     CDS             14539..15249
                     /locus_tag="ON345_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072343.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            15252..>15906
                     /locus_tag="ON345_002289"
     CDS             15252..>15906
                     /locus_tag="ON345_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072344.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDINNVITTQINAWARNNHPLLKERARQAIEN
                     "
BASE COUNT         3713 a         3911 c         4317 g         3965 t
ORIGIN      
        1 ctgttttact gctgctgttt ttagtcatga aggcgcgtat gcacgctttc ctggctttga
       61 tggtggtgtc catgggggct ggcctttttt ccggtatgcc gctcgataaa atcgcagcga
      121 cgatggaaaa agggatggga ggcaccctcg gcttcctggc ggtggttgtc gccctgggag
      181 ctatgtttgg caagatctta catgaaaccg gcgcagtcga tcagattgcc gtcaaaatgc
      241 tcaaatcctt cggtcacagc cgcgcgcatt atgccatcgg ccttgcgggg ttggtctgtg
      301 cgctaccgct gttctttgaa gtggcgatag ttctgctgat tagcgttgct ttctcaatgg
      361 cgcgccacac cggtacgaac ctggtgaagc tggtaatccc attattcgca ggcgtggcgg
      421 cagcggcggc gttcctggtg ccagggccag cgccaatgct gctggcatcg cagatgaacg
      481 ccgattttgg ctggatgatc ctgattggcc tgtgtgcggc aattccggga atgattattg
      541 ccgggccgct gtggggtaat ttcatcagcc gctacgtgga gttgcatatt cctgacgaca
      601 tcagcgaacc gcatctcggc gaaggcaaaa tgccatcttt cggattcagc ctgtcgctga
      661 tcctgttgcc gctggtgctg gtggggctga aaaccattgc cgcgcgtttt gtgccagaag
      721 gctctaccgc ttacgaatgg ttcgagttta ttggtcatcc gtttaccgcg attctggttg
      781 cttgtctggt agcgatttat ggcctggcaa tgcgtcaggg catgccaaaa gacaaagtga
      841 tggagatttg cggtcacgcg ctgcaaccgg cggggatcat tctgctggtg attggtgcag
      901 gcggcgtgtt caaacaggtg ctggttgact ctggcgtagg tccggcactg ggcgaagcgt
      961 taactggcat gggcctgccg atagccatca cctgcttcgt gctggccgct gcagtgcgca
     1021 tcattcaggg ttctgccacc gtagcctgtt taacggcggt aggactggtg atgccggtta
     1081 ttgaacaact gaactactcc ggtgcgcaaa tggcggcgct gtcgatttgt atcgctggtg
     1141 gttcgattgt tgtcagccac gttaacgacg ccggtttctg gttgttcggt aaatttaccg
     1201 gcgcgactga agccgaaacg ctgaaaacct ggaccatgat ggaaaccatc ctcggcactg
     1261 tcggtgccat cgttgggatg attgcgttcc agctgttgag ttaagtttgt tcgcccggta
     1321 gttgtgacac taccgggttc ttttcgaaaa actctcctcg ttaccccttc atccacattc
     1381 gaatgccgtc gaggaacatc tgggttgcca tcatcaccag aatcaatccc atcaggcgtt
     1441 caagtgcgtt cacccctttc tcgcccagca gacgtaaaaa tagcgaagac tgtagcagga
     1501 tgacaaaggt gccgccccag gccagcagca gagcaatcac cagatgtccc atctgattcg
     1561 ggtactgatg agacaacaac atcagcgtgg cgagaatagt cggcccggcg actaacggaa
     1621 ttgccaacgg cacgataaat ggctcttcac ctgccggaag cccgctgcta tttcctgaag
     1681 cgctggggaa aatcatctta atggcgatta gaaacagaat gatgccgcca gaaatggaga
     1741 cggtttctgc tcgcaggctg agaaatgcca gaattttctc acccgcaaac aggaacacca
     1801 gcatcaccag gagagcaata agcaactctc gcaccatgat tgcccgccgt cttttcggtt
     1861 cagtatgttt cagtacggac atgaaaatag gcaggtttcc gagcggatcc ataatcagga
     1921 tcaataaaac tgctgcagaa atgatttcat tcataactca aattccctga taattgccgc
     1981 ggactttctg cgtgctaaca aagcaggata agtcgcatta ctgatggctt cgctatcatt
     2041 gattaatttc acttgcgact ttggctgctt tttgtatggt gaaagatgtg ccaagaggag
     2101 accggcacat ttatacagca cacatctttg caggaaaaaa cgcttatgaa aaatgttggt
     2161 tttatcggct ggcgcggtat ggtcggctcc gttctcatgc aacgcatggt tgaagagcgc
     2221 gacttcgacg ccattcgccc tgtcttcttt tctacttctc agcttggcca ggctgcgccg
     2281 tcttttggcg gaaccactgg cacacttcag gatgcctttg atctggaggc gctaaaggcc
     2341 ctcgatatca ttgtgacctg tcagggcggc gattatacca acgaaatcta tccaaagctt
     2401 cgtgaaagcg gatggcaagg ttactggatt gacgcagcat cgtctctgcg catgaaagat
     2461 gacgccatca tcattcttga ccccgtcaat caggacgtca ttaccgacgg attaaataat
     2521 ggcatcagga cttttgttgg cggtaactgt accgtaagcc tgatgttgat gtcgttgggt
     2581 ggtttattcg ccaatgatct tgttgattgg gtgtccgttg caacctacca ggccgcttcc
     2641 ggcggtggtg cgcgacatat gcgtgagtta ttaacccaga tgggccatct gtatggccat
     2701 gtggcagatg aactcgcgac cccgtcctct gctattctcg atatcgaacg caaagtcaca
     2761 accttaaccc gtagcggtga gctgccggtg gataactttg gcgtgccgct ggcgggtagc
     2821 ctgattccgt ggatcgacaa acagctcgat aacggtcaga gccgcgaaga gtggaaaggg
     2881 caggcggaaa ccaacaagat cctcaacaca tcttccgtaa ttccggtaga tggtttatgt
     2941 gtgcgtgtcg gggcattgcg ctgccacagc caggcattca ctattaaatt gaaaaaagat
     3001 gtgtctattc cgaccgtgga agaactgctg gctgcgcaca atccgtgggc gaaagtcgtt
     3061 ccgaacgatc gggaaatcac tatgcgtgag ctaaccccag ctgccgttac cggcacgctg
     3121 accacgccgg taggccgcct gcgtaagctg aatatgggac cagagttcct gtcagccttt
     3181 acagtgggcg accagctgct gtggggggcc gcggagccgc tgcgtcggat gcttcgtcaa
     3241 ctggcgtaat ctttattcat taaatctggg gcgcgatgcc gcccctgtta gtgcgtaata
     3301 caggagtaag cgcagatgtt tcatgattta ccgggagtta aatagagcat tggctattct
     3361 ttaagggtgg ctgaatacat gagtattcac agccttacct gaagtgagga cgacgcagag
     3421 aggatgcaca gagtgctgcg ccgttcaggt caaaaaaatg tcacaaccag aagtcaaaaa
     3481 tccaattgga tggggtgaca caataaaaca ggaagacaag catgtccgat cgtatcgata
     3541 gagacgtgat taacgcgcta attgcaggcc attttgcgga tcctttttcc gtactgggaa
     3601 tgcataaaac caccgcggga ctggaagtcc gtgccctttt acccgacgct accgatgtgt
     3661 gggtgattga accgaaaacc gggcgcaaac tcgcaaaact ggagtgtctc gactcacggg
     3721 gattctttag cggcgtcatt ccgcgacgta agaatttttt ccgctatcag ttggctgttg
     3781 tctggcatgg tcagcaaaac ctgattgatg atccttaccg ttttggtccg ctaatccagg
     3841 aaatggatgc ctggctatta tctgaaggta ctcacctgcg cccgtatgaa accttaggcg
     3901 cgcatgcaga tactatggat ggcgtcacag gtacgcgttt ctctgtctgg gctccaaacg
     3961 cccgtcgggt ctcggtggtt gggcaattca actactggga cggtcgccgt cacccgatgc
     4021 gcctgcgtaa agagagcggc atctgggaac tgtttatccc tggggcgcat aacggtcagc
     4081 tctataaata cgagatgatt gatgccaatg gcaacttgcg tctgaagtcc gacccttatg
     4141 ccttcgaagc gcaaatgcgc ccggaaaccg cgtctcttat ttgcgggctg ccggaaaagg
     4201 ttgtacagac tgaagagcgc aaaaaagcga atcagtttga tgcgccaatc tctatttatg
     4261 aagttcacct gggttcctgg cgtcgccaca ccgacaacaa tttctggttg agctaccgcg
     4321 agctggccga tcaactggtg ccttatgcta aatggatggg ctttacccac ctcgaactac
     4381 tgcccattaa cgagcatccc ttcgatggca gttggggtta tcagccaacc ggcctgtatg
     4441 cgccaacccg ccgttttggt actcgcgacg acttccgtta tttcattgat gccgcacacg
     4501 cagctggtct gaacgtgatt ctcgactggg tgccaggcca cttcccgact gatgactttg
     4561 cgcttgccga atttgatggc acgaacttgt atgaacacag cgatccgcgt gaaggctatc
     4621 atcaggactg gaacacgctg atctacaact atggtcgccg tgaagtcagt aacttcctcg
     4681 tcggtaacgc gctttactgg attgaacgtt ttggtattga tgcgctgcgc gtcgatgcgg
     4741 tggcgtcaat gatttatcgc gactacagcc gtaaagaggg ggagtggatc ccgaacgaat
     4801 ttggcgggcg cgagaatctt gaagcgattg aattcttgcg taataccaac cgtattcttg
     4861 gtgagcaggt ttccggtgcg gtgacaatgg ctgaggagtc taccgatttc cctggcgttt
     4921 ctcgtccgca ggatatgggc ggtctgggct tctggtacaa gtggaacctc ggctggatgc
     4981 atgacaccct ggactacatg aagctcgacc cggtttatcg tcagtatcat cacgataaac
     5041 tgaccttcgg gattctctac aactacactg aaaacttcgt cctgccgttg tcgcatgatg
     5101 aagtggtcca cggtaaaaaa tcgattctcg accgcatgcc gggcgacgca tggcagaaat
     5161 tcgctaacct gcgcgcctac tatggctgga tgtgggcatt cccgggcaag aaactgctgt
     5221 tcatggggaa cgaatttgcc caaggccgtg agtggaacca tgacgccagc ctcgactggc
     5281 atctgttgga aggcggcgat aactggcacc acggtgtcca gcgtctggtg cgcgatctga
     5341 acctcaccta ccgccaccat aaagcaatgc atgaactgga ttttgacccg tacggctttg
     5401 aatggctggt ggtggatgac aaagaacgct cggtgctgat ctttgtgcgt cgcgataaag
     5461 agggtaacga aattatcgtt gccagtaact ttacgccggt gccgcgtcat gattatcgct
     5521 tcggcattaa tcagccaggc aaatggcgtg aaatcctcaa taccgattcc atgcactatc
     5581 acggcagtaa tgcaggcaat ggcggcacgg tacacagcga tgagattgcc agccacggtc
     5641 gtcagcattc actaagcctg acactaccac cgctggccac tatctggctg gttcgggagg
     5701 cagaatgaca caactcgcca ttggcaaacc cgctcccctc ggcgcgcatt acgacggtca
     5761 gggcgtcaac ttcacacttt tctccgctca tgccgagcgg gtagaactgt gtgtctttga
     5821 cgccaatggc caggaacatc gctatgactt gccagggcac agtggcgaca tttggcacgg
     5881 ttatctgccg gatgcgcgcc cgggtttgcg ttatggttat cgcgttcatg gcccctggca
     5941 acccgccgag gggcatcgct ttaacccggc gaagttgttg attgatcctt gcgcgcggca
     6001 aattgacggg gagtttaaag ataacccgct gctgcacgcc ggtcataatg aacctgacta
     6061 tcgcgacaac gccgccattg cgccgaaatg cgtagtggtg gttgatcact atgactggga
     6121 agatgatgcc ccgccgcgca cgccgtgggg cagcaccatc atttatgaag cccatgtcaa
     6181 aggattaacg tacttgcacc cggagatccc ggtcgagatc cgtggcactt ataaagccct
     6241 cgggcatccg gtgatgatca actatttgaa acaattgggc attaccgcgc tggaactgct
     6301 gccagtggcg cagtttgcca gtgaaccacg tctgaaacgc atggggctaa gtaactactg
     6361 gggttacaac ccggtggcga tgtttgcgct gcatccggcg tatgcctgct cgccagaaac
     6421 ggcgctggat gagtttcgcg atgcaatcaa agcactgcat aaagcgggta tcgaagtcat
     6481 tcttgatatc gtgctcaacc atagtgcgga actggacctc gacggcccgt tattctcgct
     6541 gcgtgggatc gataaccgta gctattattg gataagagaa gacggcgatt atcacaactg
     6601 gaccggttgc ggcaacacgc tcaatttgag tcatccggcg gtggtggatt atgccagcgc
     6661 ctgcctgcgt tattgggtag aaacctgcca cgtcgatggt ttccgctttg atctggcggc
     6721 agtcatgggc cgtacgccag agttccgtca ggatgcgccg ttgtttaccg ctatccagaa
     6781 ctgcccggtg ctctcgcagg tgaagttaat tgctgaaccg tgggatatcg ctcctggtgg
     6841 ttatcaggtg ggaaatttcc cgccgctgtt tgccgagtgg aacgatcatt tccgcgatgc
     6901 tgcccgtcgt ttctggctac attatgattt gcctctgggg gcgtttgccg ggcgttttgc
     6961 tgcctccagc gatgttttta aacgtaatgg tcgtctgccg agtgccgcga ttaatctcgt
     7021 caccgcgcat gacggtttta cgcttcgcga ctgcgtttgc ttcaaccata aacacaatga
     7081 agcaaacgga gaagaaaatc gcgacgggac caacaacaat tacagtaaca atcatggtaa
     7141 agaagggtta ggcggttctc ttgacctggt tgaacggcgg cgcgacagca ttcacgccct
     7201 gttaacaacg ttgttgctct cccagggtac gccgatgtta ctggccggtg acgaacatgg
     7261 tcacagccag catggcaata acaatgccta ctgtcaggat aaccaattaa cctggttgga
     7321 ctggtcgcag gcaagcagtg gtttaaccgc atttaccgcc gcgttaatcc atctgcgcaa
     7381 gcgcattccc gctttggtgg agaatcgctg gtgggaagaa ggcgacggca atgtccgttg
     7441 gctaaatcga tgtgctcaac ctttaagcac ggatgagtgg caaaacgggc cgaaacagct
     7501 gcaaattctg ctctcggatc gctttttgat cgcaattaac gccacgcttg aggtaacaga
     7561 gattgtttta cctgctgggg agtggcacgc cattccccca ttcgctggag aggataaccc
     7621 agtgattacg gctgtctggc agggacctgc acacggattg tgtgtgttcc agagatgata
     7681 aaaaaggagt tagtcatggt tagtttagag aagaacgatc acttaatgtt ggcgcgccag
     7741 ctgccattga aatctgttgc cctgatactg gcgggaggac gtggtacccg cctgaaggat
     7801 ttaaccaata agcgagcaaa accggccgta cacttcggcg gtaagttccg cattatcgac
     7861 tttgcgctgt ctaactgcat caactccggg atccgtcgta tgggcgtgat cacccagtac
     7921 cagtcccaca ctctggtgca gcacattcag cgcggctggt cattcttcaa tgaagaaatg
     7981 aacgagtttg tcgatctgct gccagcacag cagagaatga aaggggaaaa ctggtatcgc
     8041 ggcaccgcag atgcggtcac ccaaaacctc gacattatcc gccgttataa agcggaatac
     8101 gtggtgatcc tggcgggcga ccatatctac aagcaagact actcgcgtat gcttatcgat
     8161 cacgtcgaaa aaggcgcacg ttgcaccgtt gcttgtatgc cagtaccgat tgaagaagcc
     8221 tccgcatttg gcgttatggc ggttgatgag aacgataaaa ttatcgaatt cgttgaaaaa
     8281 cctgctaacc caccgtcaat gccgaacgat ccgagcaaat ctctggcgag tatgggtatc
     8341 tacgtctttg acgccgacta tctgtatgaa ctgctggaag aagacgatcg tgatgagaac
     8401 tccagccacg actttggcaa agatttgatt cccaagatca ccgaagccgg tctggcctat
     8461 gcgcacccgt tcccgctctc ttgcgtacaa tccgacccgg atgccgagcc gtactggcgc
     8521 gatgtgggta cgctggaagc ttactggaaa gcgaatctcg atctggcctc tgtggtgccg
     8581 gaactggata tgtacgatcg caattggcca attcgcacct acaatgaatc attaccgcca
     8641 gcgaaattcg tgcaggatcg ctccggtagc cacgggatga cccttaactc actggtatcc
     8701 ggcggttgtg tgatctccgg ttcggtggtg gtgcagtccg ttctgttctc gcgcgttcgc
     8761 gtgaattcat tctgcaacat tgattccgcc gtattgttac cggaagtatg ggtaggtcgc
     8821 tcgtgccgtc tgcgccgctg cgtcatcgat cgtgcttgtg ttattccgga aggcatggtg
     8881 attggtgaaa acgcagagga agatgcacgt cgtttctatc gttcagaaga aggcatcgtg
     8941 ctggtaacgc gcgaaatgct acggaagtta gggcataaac aggagcgata atgcaggttt
     9001 tacatgtatg ttcagagatg ttcccgctgc ttaaaaccgg cggtctggct gatgttattg
     9061 gggcattacc cgcagcacaa atcgcagacg gcgttgacgc tcgcgtactg ttgcctgcat
     9121 ttcccgatat tcgccgtggc gtgaccgatg cgcaggtagt atcccgtcgt gataccttcg
     9181 ccggacatat cacgctgttg tttggtcatt acaacggggt tggcatatac ctgattgacg
     9241 cgccgcatct ctatgatcgt ccgggaagcc cgtatcacga taccaactta tttgcctata
     9301 ccgacaacgt attgcgtttt gcgctgctgg ggtgggttgg ggcagaaatg gccagcgggc
     9361 ttgacccatt ctggcgtcct gatgtggtgc atgcgcacga ctggcatgca ggccttgcgc
     9421 ctgcgtatct ggcggcgcgc gggcgtccgg cgaagtcggt gtttactgtg cacaacttgg
     9481 cctatcaagg catgttttat gcacatcaca tgaatgacat ccaattgcca tggtcattct
     9541 ttaatattca tgggctggaa ttcaacggac aaatctcttt cctgaaggcc ggtctgtact
     9601 atgccgatca cattacggcg gtcagtccaa cctacgctcg cgagatcacc gaaccgcagt
     9661 ttgcctacgg tatggaaggt ctgttgcaac agcgtcaccg tgaagggcgt ctttccggcg
     9721 tactgaacgg cgtggacgag aaaatctgga gtccagagac ggacttactg ttggcctcgc
     9781 gttacacccg cgatacgttg gaagataaag cggaaaataa gcgccagtta caaatcgcaa
     9841 tggggcttaa ggttgacgat aaagtgccgc tttttgcagt ggtgagccgt ctgaccagcc
     9901 agaaaggtct cgacctggtg ctggaagcct taccgggtct tctggagcag ggcgggcagc
     9961 tggcgctact cggcgcgggc gatccggtgc tgcaggaagg tttccttgcg gcggcagcgg
    10021 aataccccgg ccaggtgggc gttcagattg gctatcacga agcattttcg catcgcatta
    10081 tgggcggcgc ggacgtcatt ctggtgccca gccgttttga accgtgcggc ttaacgcaac
    10141 tttatggatt gaagtacggt acgctgccgt tagtgcggcg caccggtggg cttgctgata
    10201 cggtttctga ctgttctctt gagaaccttg cagatggcgt cgccagtggg tttgtctttg
    10261 aagatagtaa tgcctggtcg ctgttacggg ctattcgacg tgcttttgta ctgtggtccc
    10321 gtccttcact gtggcggttt gtgcaacgtc aggctatggc aatggatttt agctggcagg
    10381 tcgcggcgaa gtcgtaccgt gagctttact atcgcttgaa atagttttca ggaaacgcct
    10441 atatgaatgc tccgtttaca tattcatcgc ccacgcttag cgtagaagct cttaagcact
    10501 ctatcgctta caagctgatg tttacgattg gaaaggaccc ggtcgtcgcc aataaacatg
    10561 aatggctgaa cgcaacgtta tttgctgtgc gcgatcgtct cgtggagcgc tggttacgtt
    10621 caaaccgtgc ccagttgtcg caagaaactc gtcaggttta ctacctgtcg atggagtttt
    10681 tgattggccg tacgctctcc aacgccatgt tgtcgctagg aatttacgaa gatgtacagg
    10741 gcgcactgga agcgatgggg ttaaatctcg aagagctgat tgatgaagaa aatgacccag
    10801 gcctcggtaa cggtggcctg ggacgtctgg cggcttgctt ccttgattct ctggcgacgt
    10861 tagggttgcc ggggcgcggt tacggcatcc gctatgacta cggtatgttc aagcagaaca
    10921 tcgttaacgg tagccagaaa gagtcgccag actactggct ggaatacggt aacccgtggg
    10981 aattcaaacg ccacaacacg cgctataaag tccgttttgg tggtcgcatt cagcaggaag
    11041 gtaaaaaaac gcgctggatt gaaaccgaag agattctggg agtcgcttac gatcagataa
    11101 tccctggtta cgacaccgac gcgaccaaca cgttgcgttt gtggagtgcg caagccagta
    11161 gcgaaattaa cctcggtaaa ttcaaccagg gtgactactt cgcggcagtg gaagataaaa
    11221 accactccga gaacgtatct cgcgtactgt atccagatga ctccacctac tccgggcgtg
    11281 agctgcgcct gcgtcaggaa tacttcctgg tttcctcgac cattcaggac attttaagcc
    11341 gccattatca gttgcataaa acctacgata acctggcgga taaaatcgcg attcatctca
    11401 atgataccca tccggtactg tcgattcctg agatgatgcg tctgctgatc gatgagcacc
    11461 aatttagctg ggacgacgcg tttgaggtgt gttgtcaggt cttctcctac actaaccaca
    11521 cgctgatgag cgaggcgctg gaaacctggc cggttgatat gctgggtaaa attctgccgc
    11581 gtcacctgca gatcatcttt gaaatcaacg actatttcct gaaaaccttg caggaacagt
    11641 atccgaacga taccgatctg ctgggacggg cgtcgatcat tgatgaatcc aacggtcgtc
    11701 gtgtgcgtat ggcctggctg gcggttgttg tgagccacaa agttaacggt gtatcggaac
    11761 tgcactctaa tctgatggtg caatcgttgt ttgccgactt tgcgaaaatc ttcccgggtc
    11821 gtttcaccaa cgtcaccaac ggtgtgacgc cgcgtcgctg gctagcggta gcgaacccat
    11881 cgctttcagc cgtgctggac gaacacctgg gccgtaactg gcgcaccgac cttagcctgc
    11941 ttaatgagct gcaacaacac tgtgatttcc caatggttaa tcacgctgtg catcaggcga
    12001 agctggagaa caaaaagcgt ctggcagagt atatcgccca gcagctgaat gtggtggtga
    12061 atccaaaggc gttgttcgat gtacaaatca aacgtattca cgaatacaaa cgtcaattga
    12121 tgaatgtgtt gcatgtgatt acccgctata accgcatcaa ggccgacccg gatgcgaagt
    12181 gggtaccgcg cgtgaatatt tttggcggta aggcggcttc ggcctattac atggcgaagc
    12241 acattattca tttgatcaat gacgtagcga aagtgatcaa caacgatccg cagattggcg
    12301 ataagctgaa agtcgtgttc atcccgaact acagcgttag cctggcgcag ttgatcattc
    12361 cggcggcaga tctgtctgaa cagatttcgc tggcagggac ggaagcttcc ggcaccagta
    12421 acatgaagtt tgcgcttaac ggcgcgctga ctatcggtac gctggacggt gcgaacgtcg
    12481 agatgctgga tcatgtcggt gctgacaata tctttatctt tggtaacact gcggaagaag
    12541 tggaagaact gcgtcgtcag ggctacaaac cgcgtgaata ctacgagaaa gatgaggagc
    12601 tgcatcaggt gctgacgcaa atcggcagcg gtgtattcag tccggaagat ccgggtcgct
    12661 atcgcgatct ggttgattcg ctgatcaact tcggcgatca ctatcaggtt ctggcggatt
    12721 atcgcagcta tgtcgattgt caggataaag tcgacgaact ctacgagctt caggaagagt
    12781 ggaccgcaaa agcgatgctg aacattgcca atatgggcta cttctcttct gaccgtacta
    12841 tcaaagagta cgccgatcat atctggcata tcgatccggt gagattgtaa gttcaccaat
    12901 aaatagaacg gggccaaagg gtcccgtttt tttccgccat catttcggaa aagtgtccag
    12961 aaggggcaag gggactcggg gagtataatt tcaccattca aaaagaatgg aaagaataat
    13021 gcaaaacaga aaatggattt tgtcctcgct ggtaatgact tttttcggca tccccatact
    13081 gacgcaattt ttggcggcgg ttgttgctat gctgggggtc ggacttgccg gtattattga
    13141 agtttgtaat atccttatca cgccaacaag ttaccttctg ctcaacattt ttatgctggc
    13201 gctgggcgca ttaatgctat ttttctcggg gcgagtatgg gcaggcgata gtgcaccaga
    13261 aaaaagagaa atagccgtct ggcgacaatg tcttttttta gtacccggtt tattaatcct
    13321 ggttggctgg ataatcgcgc tgcatctggc agattatcaa tttcaccaga tgggatcggg
    13381 ttggttggct gatcttatgc tcccctggct gggcgttttg ttagtctcag tagtcggagg
    13441 tgagtactgg tggatagtca ttatacccgt tggcgcgcat atcagttttt cgctggggta
    13501 cggccggcca accagacatc ctttaaccgg tacatccggg ctacgttgcc gtaattccct
    13561 cctgtttatc cttctcatgc ttggttttgt cgctggttat cagggttatt tatataaaca
    13621 gctcaatccc ggcgtcggtg tgcgcgaaaa tattgatacc tgggcctggc gacccgataa
    13681 actcaataat caactgacac cactgcgtgg taaaccgcaa attcagttta cgcaaaactg
    13741 gccgcgactc gatggtgcta cggcggcgta cccaatttat gcctctgcat tttatgctta
    13801 agcgttatac cggaagattt tcacaccagg gaatatctgg agagctctcg tacccccgat
    13861 gcgtataaca ggattgttaa aggtgatgct gatattattt ttgtggcgca accttccggt
    13921 gggcagaaaa aacgcgctga ggaatcgggc gttactttgc tgtacacgcc ttttgcccgt
    13981 gaagcgtttg ttttcatcgt caatgcggat aatccggtta attccctgac tgaacaacag
    14041 gtgcgtgaca tcttcagtgg tgcaattacc aactggcgta ctgttggcgg taacgatcag
    14101 gagatccaga cctggcaacg cccggaagac tctggtagcc agacggtgat gcaatcccag
    14161 gtcatgaaaa atgtccgcat gatctcgccg caggaaacag aagtggcaag tatgatggag
    14221 ggaatgatta aagtcgttgc cgaataccgt aatacaaaca acgcaatagg ttacaccttc
    14281 cgttattacg caacacaaat gaacgccgat aaaaatataa aactgctagc gattaacggt
    14341 attgcaccga ctgccgaaaa cattcgcaac ggcaaatatc cctatatcgt cgatgccttt
    14401 atggtaacgc gggaaaatac gacgtcagag acacaaaaat tgttggagtg gtttttaacg
    14461 ccgcagggac agagtttggt ggaagatgta ggttatgtcc cgatgtataa gacgctacct
    14521 taaagctgga taataaaaat ggaattagtt aatacattat ttgcaagcct tgccggtact
    14581 gatcccttca caggcgtgga tattacgatt gccaactgca aatcggcata ctgggatgaa
    14641 ggcattgttc agcaacttat taaccaggcc ctggatgaag gggaaaagtt cgtcggtgct
    14701 gacggtcttg agggattgct tcgatatgat gtcacattaa atattggctt aacctccagc
    14761 aaggtatggc cgggattttc cctcgatacc gccaccatta gccgtctttg tgcctgtggt
    14821 gctgattttg gatttgaccc ctatatcagt gatgtaccag atgttcaatg tgacctgaac
    14881 acgacaaatg acgtcaccgt acaatttacg gcgatgctaa atccggatga gcgggtgata
    14941 atagcaaagc gcccgcttaa aaaatgtgac tcctggattg aggatgtcta tatctttcag
    15001 gtctttaaag atgcctggaa atttcataac aataacagtt tacgcggatt tcgcgataag
    15061 caggccgaac tgaaactcta cacgcgtcat tactcagtgg aaaattgcgc agaggaaagc
    15121 tgtcgagact gcaatagttg cattcgcccc agcttttccc tttcgcgtag cgcccttatt
    15181 cgcctgaacg cggcgaatgc tcgttttgtc tatcaaccgt ttacacgcga tcagcgggcg
    15241 agggggtaac tatgcaaccc agccccgcca tgcaggcatt aattgagcag atttatcaca
    15301 cctttcgtcg ttatcgggtg ccgcagaaat ttgtggtttg ttgtgaatat tgcctgagtc
    15361 agcaggaaca aaaggcatta cggagtacat cgttacgagc gatcccgtat tccttaatca
    15421 atgcctggaa tagtagccca gggcctgatc cgcaaaatag cgatgaggtc cgctattttc
    15481 tgccgcgttt actggaattt gttgcacagg ggcattttga caatattcac gtagtttttt
    15541 ctctgcgccg tatcaacctt gccaacaaag aaaactggcg tgcggatgaa cgggcaattt
    15601 tacagcgatt tgcctgccag tatatgactg attgggtatc cggcgatgaa gctgtcgaat
    15661 tacagtatat gctggaaatg tttttccgcg ccgatatcgc tcttgcgcca ttgctggatg
    15721 ccattaattc agttcccggc ttttggtcga ccgtttctct tgcctgttta ttgaacagat
    15781 attgtgagga ttatatcaga gataaccagg acgatatcaa taacgtcatt accacccaaa
    15841 tcaatgcctg ggctagaaat aatcatcctt tactgaaaga aagagcgcgg caagcgattg
    15901 agaacc
//
LOCUS       DAJSKJ010000083        15871 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000083 DAJSKJ010000000
VERSION     DAJSKJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.083"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..602
                     /gene="dgcP"
                     /locus_tag="ON345_002290"
     CDS             <1..602
                     /gene="dgcP"
                     /locus_tag="ON345_002290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR3072345.1"
                     /translation="LQLFAGLIAQYIQKEALVEQLREANAALIAQSYTDSLTGLPNRR
                     AIFENLTTLFSLARHLNHKIMIAFIDLDNFKLINDRFGHNSGDLFLIQVGERLNTLQQ
                     NGEVIGRLGGDEFLVVSLNNENADISSLRERIQQQIRGEYHLGDVDLYYPGASLGIVE
                     VDPETTDADSALHAADIAMYQEKKHKQKTPFVAHSAPHC"
     gene            635..733
                     /locus_tag="ON345_002291"
     CDS             635..733
                     /locus_tag="ON345_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR3072346.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            736..810
                     /gene="yoaJ"
                     /locus_tag="ON345_002292"
     CDS             736..810
                     /gene="yoaJ"
                     /locus_tag="ON345_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR3072347.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(869..1117)
                     /locus_tag="ON345_002293"
     CDS             complement(869..1117)
                     /locus_tag="ON345_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3072348.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(1265..1447)
                     /locus_tag="ON345_002294"
     CDS             complement(1265..1447)
                     /locus_tag="ON345_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR3072349.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(1451..1810)
                     /gene="yeaR"
                     /locus_tag="ON345_002295"
     CDS             complement(1451..1810)
                     /gene="yeaR"
                     /locus_tag="ON345_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCR3072350.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1983..2621)
                     /gene="leuE"
                     /locus_tag="ON345_002296"
     CDS             complement(1983..2621)
                     /gene="leuE"
                     /locus_tag="ON345_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCR3072351.1"
                     /translation="MFAEYEVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2748..3671)
                     /gene="dmlR"
                     /locus_tag="ON345_002297"
     CDS             complement(2748..3671)
                     /gene="dmlR"
                     /locus_tag="ON345_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR3072352.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3774..4859
                     /gene="dmlA"
                     /locus_tag="ON345_002298"
     CDS             3774..4859
                     /gene="dmlA"
                     /locus_tag="ON345_002298"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR3072353.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            5110..6721
                     /gene="yeaV"
                     /locus_tag="ON345_002299"
                     /pseudo
     CDS             5110..6721
                     /gene="yeaV"
                     /locus_tag="ON345_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
     gene            6753..7877
                     /gene="yeaW"
                     /locus_tag="ON345_002300"
     CDS             6753..7877
                     /gene="yeaW"
                     /locus_tag="ON345_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR3072354.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWTFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7933..8898
                     /gene="yeaX"
                     /locus_tag="ON345_002301"
     CDS             7933..8898
                     /gene="yeaX"
                     /locus_tag="ON345_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR3072355.1"
                     /translation="MSDYQMFEVQVSQVEPLTEKVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAVD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8952..10067)
                     /gene="rnd"
                     /locus_tag="ON345_002302"
     CDS             complement(8952..10067)
                     /gene="rnd"
                     /locus_tag="ON345_002302"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR3072356.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10149..11834)
                     /gene="fadD"
                     /locus_tag="ON345_002303"
     CDS             complement(10149..11834)
                     /gene="fadD"
                     /locus_tag="ON345_002303"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR3072357.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(12039..12620)
                     /gene="yeaY"
                     /locus_tag="ON345_002304"
     CDS             complement(12039..12620)
                     /gene="yeaY"
                     /locus_tag="ON345_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR3072358.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(12660..13355)
                     /gene="tsaB"
                     /locus_tag="ON345_002305"
     CDS             complement(12660..13355)
                     /gene="tsaB"
                     /locus_tag="ON345_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR3072359.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13413..15323)
                     /locus_tag="ON345_002306"
     CDS             complement(13413..15323)
                     /locus_tag="ON345_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR3072360.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPLTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15455..15799
                     /locus_tag="ON345_002307"
     CDS             15455..15799
                     /locus_tag="ON345_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR3072361.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGCNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3884 a         4143 c         3908 g         3936 t
ORIGIN      
        1 ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca ctggttgaac
       61 agctgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg ttaaccgggt
      121 tgccgaatcg gcgggcgatt tttgaaaatc tgacgacgct gttttctctc gcccggcatc
      181 ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta atcaatgatc
      241 gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc cttaatacgc
      301 tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg gttgtttcac
      361 taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag caaatacgtg
      421 gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt ggcatagtag
      481 aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat attgcgatgt
      541 atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg ccacattgct
      601 gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta tttttccggt
      661 tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg gctatgctgc
      721 cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc gattattgtc
      781 gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc
      841 tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag ataaatagca
      901 cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca tcgactttac
      961 caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc cccagcagga
     1021 tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc agaataccgg
     1081 caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt atctgttttg
     1141 cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc aagatttgtg
     1201 acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca acgatcagtg
     1261 ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc acggtattca
     1321 ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc ggcgtctctg
     1381 tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta tacgttgctt
     1441 tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct tccatcagga
     1501 cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg gcttcaatgt
     1561 tgtgccactt ttctggaggg aacaccgcaa actgccccgc ttcgataagg atcacctgat
     1621 caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca tgcataacgg
     1681 aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg aatattccgg
     1741 caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa ttctgtggga
     1801 tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat tccagccgtt
     1861 tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt tttagcacca
     1921 tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc aggctgaatg
     1981 tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa accaatcagt
     2041 gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac aaaagcacca
     2101 gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata
     2161 aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa aaacgacaca
     2221 tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt aaaaatggcc
     2281 ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt cgcgtagaga
     2341 attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat gttgaataat
     2401 atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat caatactgca
     2461 tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct actgacgcta
     2521 tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat ggccccaacc
     2581 agataggtcc agtaattcag aacctcgtat tcagcgaaca cattaacctc tttaattatc
     2641 tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc ttagaaagga
     2701 gaaaatcagg ttttaacccg atatcaaccc gataattgaa tcattaatca ggcatgctcc
     2761 agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctac cgttatccgc
     2821 actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc tggctggtaa
     2881 tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac atcccaccag
     2941 gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc tccatgatta
     3001 gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg taattgccag
     3061 acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga aagatcggtt
     3121 aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag aatgcggtaa
     3181 ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat gcgaatatcg
     3241 agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa acgtaattcc
     3301 agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg tcgtccaaaa
     3361 ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc agaaagttcg
     3421 tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat ccgctctcct
     3481 tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc
     3541 tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc ttccgccacg
     3601 gcggcaaaac cagcccgacg agccaccagc ataaagacgc gcaaatcatt cagcagcggt
     3661 aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt ccttttcagt
     3721 caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac ccaatgatga
     3781 aaacgatgcg tattgctgcg atcccgggag acgggattgg caaagaagtc cttcctgaag
     3841 ggattcgcgt gttacaggct gccgctgagc gctggggctt cgacttgagt tttgagcaaa
     3901 tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg gacgactggc
     3961 atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg ccggataccg
     4021 ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt
     4081 acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac
     4141 agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc
     4201 tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa tcggtattta
     4261 cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa agccgcccac
     4321 gtaagacgct aacttctgcc actaaatcga acggtttagc catcagcatg ccgtactggg
     4381 atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac aagcagcata
     4441 ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt
     4501 ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc accattggca
     4561 ttgccccatc cgctaatctg aatccggaac gcactttccc gtcgctcttc gagcctgtcc
     4621 acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc acgatttggg
     4681 ccggggcaat gatgctcgat tttctcggca atggcgatga gcgtttccag caagcgcata
     4741 acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca ccggatatga
     4801 aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt ttgcgttaag
     4861 gtcaaacctg tttatttgaa ccgcgtcact gacgcggttt ttttattcgt tctttgcagt
     4921 taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg gctttccgta
     4981 ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca caacatacaa
     5041 ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt tatgcccagg
     5101 agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag catcagcctg
     5161 gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc ggccgacgcg
     5221 gccaacgcaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt
     5281 ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa tattcgtctt
     5341 ggcgaaggaa aaccggaata cagcacgctc tcctggctgt tttatgttta tttgtgccgg
     5401 tttaggttct tctacgcttt attggggggt tgctgaatgg gcctattatt atcaaacacc
     5461 tggattaaat atcgcaccgc gttcacaaca ggcactcgaa tttagcgttc cctactcttt
     5521 cttccactgg ggcatcagcg cctgggcaac ttatacgctg gcctcattaa tcatggctta
     5581 tcactttcat gtgcggaaaa acaaaggtct gagcctttcc ggcattattg ctgctattac
     5641 cggcgttcgc ccgcaaggcc catggggaaa actggtcgat ttgatgttcc tgatcgccac
     5701 tgtcggcgca ctgaccattt cccttgttgt taccgcagca acctttaccc gtgggctttc
     5761 cgcgctgacc ggtttacccg ataacttcac cgtgcaggca tttgtgatcc tgctttccgg
     5821 cggcattttt tgcctaagct cgtggtttgg tatcaacaac ggtttgcaac gtctgagcaa
     5881 aatggttggc tggggcgcgt tcctgctgcc attactggtg ctgattgtcg gcccaaccga
     5941 atttattacc aacagcatca tcaatgccat cggcctgacc acgcaaaact tcctgcaaat
     6001 gagcttattc accgatccgc ttggcgatgg ttcatttacc cgcaactgga ccgttttcta
     6061 ctggctgtgg tggatctcat acacccctgg cgtagcaatg tttgtcaccc gcgtttcccg
     6121 cggtcgtaag attaaagaag ttatctgggg actgatcctc ggcagcaccg tcggttgctg
     6181 gttcttcttt ggcgtaatgg aaagctatgc cattcatcag tttatcaatg gcgtaatcaa
     6241 cgtcccacag gtgctggaaa cactgggcgg cgaaacagct gtacagcaag ttctgatgtc
     6301 gttgccagcc ggtaaattgt tcctcgccgc atacctgggc gtgatgatta ttttccttgc
     6361 ctcgcatatg gatgcagtgg cctacaccat ggcggcgacc agtacgcata atctccagga
     6421 aggtgacgat cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca ctctgatccc
     6481 gctttccatc ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg tcgtgctcac
     6541 agcccttccc ttcctcgtca ttttactggt gaaagtcggc ggatttattc gctggctgaa
     6601 acaggattac gccgacattc cggctcatca agttgaacat tatctcccgc agacaccggt
     6661 tgaagccctg gaaaaaacgc cagtgctccc tgcgggaacc gtattcaaag gcgacaactg
     6721 agcgccatca tcctaacgat aaaggtatcc ctatgagcaa tctgagccct gactttgtac
     6781 tacccgaaaa tttttgcgct aacccgcaag aggcgtggac cattcctgcc cgtttttata
     6841 ccgatcagaa cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc tggatttgcg
     6901 tcgctcacag cagcgaactg gcgaatgcca atgattatgt gacgcgtgag atcattggcg
     6961 aaagcatcgt gctggtacgc ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc
     7021 cgcaccgtgg tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg attacctgcc
     7081 cgtatcacgc atggacattc aaactcgatg gcaacctggc ccatgcacgt aactgcgaaa
     7141 acgtcgccaa tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg gaagaatatg
     7201 ccggattcgt cttcatcaac atggacccca acgccaccag cgtagaagat cagttacccg
     7261 gtctgggcgc gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc
     7321 gctttaccac ccgcacgcct gccaactgga agaacattgt cgataactat ctcgagtgct
     7381 atcactgtgg tccggcgcat ccaggtttct ccgactccgt acaggttgat cgttactggc
     7441 acaccatgca cggtaactgg acgctgcaat acggtttcgc caaaccgtcc gaacagtcgt
     7501 ttaaatttga agagggtacg gatgcggcat tccacggttt ctggctgtgg ccgtgcacga
     7561 tgctgaacgt caccccgatc aaagggatga tgacggtcat ttatgaattc ccggtggatt
     7621 ctgaaactac cctgcaaaac tacgatattt acttcaccaa tgaagagtta accgacgagc
     7681 aaaaatcgct gattgagtgg tatcgcgatg tgttccgtcc ggaagattta cgtctggttg
     7741 aaagcgtaca gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg
     7801 acagtagcgg tagtggcatt tccgaacatg gtatcgccca tttccataat ctgctggcgc
     7861 aggtgtttaa ggactaatga catcggcggc ggtattttcc gccgctgggc tgatttttga
     7921 tggagtacag caatgtcaga ctatcaaatg tttgaagtac aggtgagtca ggtggaaccg
     7981 cttaccgaaa aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc gctgcctgcg
     8041 ttcaccggag gaagtcacat cattgtgcag atgagcgatg gtgataacca gtacagcaat
     8101 gcgtattcac tactgagttc gccgcatgac acctcttgtt atcagattgc cgttcggctg
     8161 gaggaaaact cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt gggcgatcgg
     8221 ttaacgattt caacgcctaa taacctgttt gcgctaattc cctcagccag aaagcatctg
     8281 tttatcgcgg gcggtattgg tatcacccct ttcctgtcgc acatggcaga gctgcaacac
     8341 agcgacgtcg actggcagct acattactgc tcgcgaaatc cagaaagttg cgcatttcgt
     8401 gatgagctag tccagcatcc gcaggctgag aaagtccatt tgcatcattc atcaaccgga
     8461 acacgactgg aattagcgcg attattggcg gatatcgaac ctggcacaca cgtttatacc
     8521 tgtggccccg aggcgctaat tgaagcggta agaagtgaag ctgcgcgtct ggacatcgcc
     8581 gtcgatacgc tgcactttga gcaatttgct atcgaagaca aaaccggcga tgcatttacc
     8641 ctggtgcttg cccgttccgg aaaagagttt gtggtgccgg aagagatgac tattttgcag
     8701 gttattgaaa ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga
     8761 acctgcgaaa cagcaatact ggaaggtgaa gctgaccatc gggatcaata ttttagcgat
     8821 gaagagcgtg ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa gggtaaacgc
     8881 ctggtgttgg atttgtagtt tgccgaagcc ggatgtggcg ctgagcgcgc ccagtccggc
     8941 ttcggaagat tttactgcgg atattcctgc aataaattgt gtaatgcttc cgccatcagc
     9001 tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag tttccagtgc
     9061 cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct gatcttatgc
     9121 gtttcgctca cgtcagtaat cagcgactta atcgctttaa acgctttacg ataacccggc
     9181 atgtccatca ggttaagcat cggctgcggt aaggcatctt ccggcaatgt ctgcgctttt
     9241 tccaccagcg ccagcagcgt tttaccgtga aagcggattt cgctaccgga taaacccagg
     9301 ctgtccagtt cgcctaaact tcccggcata taacgcgcta ccgaccacaa atgctcttca
     9361 cgcacgacaa agttcaccgc cagatcgcgc tctcgcgcct tgcgcagtcg ccagtcggct
     9421 aacagttgca gacaggccag ttggcgtgtg cgtaattgcc aggcattggt gatatcacgc
     9481 caggcatctt ccggcgcaac gacttcctga cgacgcattt gcatcaggcg gcattcatcc
     9541 agcgccgcag gtagccagcc ggaggcctcc gtttctacca taagcttggc ggtgatcggt
     9601 aacagatacc agacatccgc cgctgcgtat tcacactgac gttcggtcag cggtctggcc
     9661 agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc ttccaccatg
     9721 gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc
     9781 aagggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc actgcctgca
     9841 tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg
     9901 atcccgagtg gatcgattag cgccagatgc tcgccatcga aaagttgaat caaccccagc
     9961 tgcgggtaat aagtgcgcgt acgaacaaat tcagtatcca gggctatcgc cggaaaggca
    10021 cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg gtaattcaaa
    10081 ttgttttctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga
    10141 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
    10201 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
    10261 ttgtatcccg tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga
    10321 tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
    10381 gccgcgacct cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
    10441 acgttaaaac cggaaaccag aatcatgtct tttttacgat caacaatgcg caggaatcct
    10501 tcttcatcca ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg
    10561 gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca
    10621 cccggttgac ctggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac
    10681 ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc
    10741 agcggcgcac actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc
    10801 caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg
    10861 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
    10921 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc
    10981 gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc
    11041 agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc
    11101 aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc
    11161 atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc
    11221 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat
    11281 gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa
    11341 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
    11401 tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca
    11461 atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
    11521 ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
    11581 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa cccttgttgc
    11641 aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat tacctccccc
    11701 atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
    11761 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
    11821 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
    11881 gatatacatg ccgttaacat aatattaact catcatacca gcttgataat tacccaacga
    11941 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
    12001 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt
    12061 actctcgcgg ggccgggatt ataccagccc catccgccgt agccataggg ccagcctcga
    12121 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
    12181 caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtgtt gccgattttt
    12241 ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc
    12301 gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc
    12361 ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg
    12421 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
    12481 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgctgccttt aatggcgtcc
    12541 ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata
    12601 acattctttt gaaccgccat ggtgcgactc ctttttttct cagggcatac tcttaagatt
    12661 cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca
    12721 tgttccaccg ccaccgtttt accctcggca aacatctgac acgcaatcgg cagcatatct
    12781 tcagcagcag gcagtaacac ttcgccatcg cgcaaaacca gcccgctctc tttaccgaga
    12841 tccggccagg cttgccagcc cgtccctacc gtcacccatt cgccggaaag ctgttgcatt
    12901 cgttcatgga cgatttcggg tttgagtacg gcttcggttt cttcaccgtg ccagataccg
    12961 ttttcatcac gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc aatggctgac
    13021 agcacgcgtg ttgcgccgtt tttgcgccac gccccttgcg ccatcgtcat tagcgtggag
    13081 acgccaatca tcggtaattc cgcgccaagc gccagccctt gcgcgatgcc aataccaatg
    13141 cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgtttat gtcagtcagg
    13201 gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca
    13261 cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag
    13321 gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa
    13381 aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa
    13441 cgaaccgcac gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc
    13501 gcgccgtaag ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg
    13561 gcgtcgcgaa tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt
    13621 acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagt
    13681 ggatcatccg gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa tgtatcgcca
    13741 cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcatt accggcactg
    13801 acaaactgct gcaacagttg gcctttactg gtttccccct gcaacaaaac gggaagcgtc
    13861 atggtagcgc ggaactgctc ggccagatcg cgcatcatgg cgtgcgaggt acaaagcata
    13921 aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca
    13981 gaacctggct ggttggttag cggcagattg cgcggcacac agagtaacgc ctggcggctg
    14041 taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg
    14101 aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc
    14161 ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta
    14221 aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc
    14281 cgcttcagcc gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa
    14341 cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa
    14401 aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc
    14461 tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt
    14521 aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg
    14581 agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg
    14641 tgggcttcgt cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct
    14701 ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc
    14761 gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga
    14821 cagttgtcgt tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg
    14881 caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg
    14941 cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg
    15001 cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt
    15061 gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag
    15121 ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta
    15181 ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc
    15241 gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg
    15301 tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacaggga ttatgtcagg
    15361 atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc
    15421 agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga
    15481 agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga
    15541 aaacctcgac gccgatgctt ttgagcaaac cgccaacacg ctggcgcaga ttgacgccgt
    15601 gctggaaaaa cagggctgca ataaatcgag cattctggat gccaccattt tcctggctga
    15661 taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc
    15721 gccggtgcgc tgcacggtgc aagcggggct gatgaacccg aagtataaag ttgaaattaa
    15781 gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat
    15841 aatactggtc ggttttacat aaatcgacca g
//
LOCUS       DAJSKJ010000084        15555 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000084 DAJSKJ010000000
VERSION     DAJSKJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.084"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..769
                     /locus_tag="ON345_002308"
     CDS             <1..769
                     /locus_tag="ON345_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCR3072362.1"
                     /translation="DFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGAL
                     DGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQT
                     QQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNN
                     ALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTL
                     LMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            766..1389
                     /locus_tag="ON345_002309"
     CDS             766..1389
                     /locus_tag="ON345_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR3072363.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            1323..5927
                     /locus_tag="ON345_002310"
     CDS             1323..5927
                     /locus_tag="ON345_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR3072364.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLANADDSLPS
                     SNYAPPAGGTFFLLADRSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFHDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVNVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSCRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFSV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQLPVTYEWLRLEDRTSHSGELPSGGKSFTVNFD
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGV
                     VDEMIYALQPEIAPNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSER
                     RVKMLERPRREEVDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQ
                     GRAYLRSEKNLYMKWSMPTVYCVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLT
                     LHKGANYISLTQNIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGG
                     ENALMLPEQASNIRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLA
                     DHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQA
                     LGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRG
                     TKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAW
                     DKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQSTIERALAMNWLAKYMATMPP
                     VVLPAPAGAWAKHKLTGGGEYWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQS
                     NIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVE
                     VPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRH
                     LLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            5928..7577
                     /locus_tag="ON345_002311"
                     /pseudo
     CDS             5928..7577
                     /locus_tag="ON345_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            7582..8358
                     /locus_tag="ON345_002312"
     CDS             7582..8358
                     /locus_tag="ON345_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR3072365.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            8633..11482
                     /gene="rcsC"
                     /locus_tag="ON345_002313"
     CDS             8633..11482
                     /gene="rcsC"
                     /locus_tag="ON345_002313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR3072366.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(11682..12332)
                     /gene="rcsB"
                     /locus_tag="ON345_002314"
     CDS             complement(11682..12332)
                     /gene="rcsB"
                     /locus_tag="ON345_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR3072367.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     MFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(12349..15021)
                     /gene="rcsD"
                     /locus_tag="ON345_002315"
     CDS             complement(12349..15021)
                     /gene="rcsD"
                     /locus_tag="ON345_002315"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR3072368.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LSLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPK
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         3771 a         3802 c         4184 g         3798 t
ORIGIN      
        1 cgattttacg ccggtctgga acagtatgcc tgccggggcc agcttctgtg tggtggtgcc
       61 gtattcacac ggtattgccg aagagatgct ttcgcacatc agccaggaaa acgacaagct
      121 gaatggggcg ttagacggtg ccgcggggct gtgctggtat gaagactcaa aattgcaaac
      181 cccgctgttt gtcggtcagt ttgatggcac tgccgaacag gcgcaattgc cagggaaact
      241 gtttacgcaa aatattggtg cgcacgaaag caaagcgcca gaaggtgttt tgccgataag
      301 ccagactcag cagggcgaag cgcaaatctg gcgtcgcgaa gtgagttccc gatacggtca
      361 gtatccgaaa gcgcaggcgg cgcaacccga tcaattaatg tcggattatt ttttccgcgt
      421 gtcgctggcg atgcaaaaca aaacgctgct tttctccctc gatgacacgc tggttaataa
      481 cgccctgcaa acactgaata aaaaccgccc ggcaatggtg gatgtaatac ccactgatgg
      541 catcgttccg ctctatatca atccacaagg cgtggcgaaa ctgctgcgta acgaaacgtt
      601 gaccagtctg ccgaagaatc tcgaaccggt tttttataac gccgcacaaa ctttattaat
      661 gccgaagctg gacgctttat ctcaacaacc gcgttatgtc atgaagctgg cccagatgga
      721 acccggtgcc gcctggcagt ggctacccat aacctggcag ccgctatgag gcacgggctg
      781 ctggcgctga tttgctggct gtgttgtgtt gttgcccata gcgaaatgct gaatgtcgaa
      841 caatccgggc tgtttcgcgc ctggtttgta cgcattgcac aagaacagct ccgccagggg
      901 ccaagtccac gctggtatca gcaggattgt gcgggcctgg tgcgatttgc ggcgaacgag
      961 acgctgaaag ttcacgatag taaatggcta aaaagtaacg gtttctctag ccagtatttg
     1021 ccgccagaga tgacgctaac acctggacag cgtcaactgg cgcaaaactg gaatcagggg
     1081 aacgggaaaa ccggccccta cgtgaccgcg attaatttga ttcagtacaa cagccagttt
     1141 attggccagg acataaacca ggcgctgcct ggcgatatga tttttttcga tcagggcgat
     1201 gcccagcact taatggtctg gatggggcgt tacgtcatct accacaccgg aagcgccacg
     1261 aaaactgaca acggaatgcg cgcagtcagt ctgcaacaac ttatgacatg gaaggacacc
     1321 cgatggatac ccaacgattc caatcccaat ttcattggca tttatcgttt aaattttctg
     1381 gcgcgatagc agcgtgcttg tccctgtctc ttgtgggaac agggcttgct aatgctgatg
     1441 attcgcttcc ttccagtaac tatgcgccgc ccgccggggg aacattcttt ttgcttgctg
     1501 acagaagttt tagcagcagt gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt
     1561 atcggcgcta tcagatggaa gagtacggcg gcgtggacgt tcgcctgtat cgtattcctg
     1621 acccgatggc atttttgcgc cagcagaaaa acctgcatcg cattgtggtg caaccgcaat
     1681 atctgggcga cgggctgaac aatacgctga cctggctgtg ggataactgg tacggcaaat
     1741 ctcgccgcgt aatgcagcgt actttctctt ctcagtcacg gcagaatgtg actcaggcat
     1801 tacccgaatt acatctcggc aatgccatta ttaaaccttc ccgttatgta cagaacaacc
     1861 agttttcccc gctgaaaaaa tatcccttgg tggaacagtt ccgttatcca ctatggcagg
     1921 ctaaaccggt cgagccgcag caaggggtaa aactggaagg cgcatccagc aatttcatct
     1981 cgccgcagcc gggtaacatt tatattcctc tcggccaaca agagccggga ctgtacctcg
     2041 tcgaggcgat ggttggtggg tatcgggcga cgacggtggt gtttgtttcc gataccgtgg
     2101 cgcttagcaa agtgtcaggc aacgagcttc tggtgtggac cgcgggtaaa aaacagggtg
     2161 aagcgaagcc cggctcagag atcttgtgga ctgacggtct tggcgtgatg acccgcggtg
     2221 tgaccgatga cagcggtacc ttgcagttac aacatatatc gccagaacgc tcatacattc
     2281 tgggtaagga tgctgaaggc ggcgtttttg tctccgagaa cttcttctac gaaagtgaaa
     2341 tctacaacac ccgcttgtat atctttaccg atcgcccgct atatcgcgca ggcgatcgtg
     2401 tcgatgttaa agtgatgggc cgcgagttcc acgatccgtt gcattcatcc cccatcgtca
     2461 gcgccccggc gaagctttcg gtgctggacg ccaacggcag tctgttgcaa accgtcaatg
     2521 tcacgctgga tgcgcgcaat ggcgggcagg gaagtttccg cctgccagaa aatgccgttg
     2581 ccggaggtta tgagttacgt cttgcttacc gcaatcaggt ctatagcagc agttgtcgcg
     2641 tggcaaacta catcaagcca catttcgaga ttggtttagc tctcgacaaa aaagagttca
     2701 aaactggcga agcggtcagc ggcaaactgc aactgctcta cccggatggc gagccggtaa
     2761 aaaatgcccg cgtgcagtta agtttgcgcg ctcagcaatt atcaatggtc ggtaacgatt
     2821 tgcgttatgc cggacgtttc tccgtgtcgc tggaaggcag cgaaacggtg tccgacgcca
     2881 gcggtcatgt ggcgttaaat ctccccgccg ccgataaacc gagccgttat ttgttaaccg
     2941 tctccgccag tgacggcgcg gcgtatcgtg tcaccaccac caaagagatc ctcattgaac
     3001 gcggtctggc gcattactca ttaagtactg ccgcacaata cagtaatagc ggcgagtcgg
     3061 ttgtgttccg ttatgccgcg ctggaatctt caaaacagct tcctgttacg tatgaatggt
     3121 tgcgtctcga agaccgcacg agccatagcg gagagctacc gtcaggcggc aaatccttta
     3181 ccgtcaattt cgataaacct ggcaactaca atctgacatt acgcgataaa gacggcttaa
     3241 ttctcgctgg gttaagtcat gccgtcagcg gtaagggcag cacggcgcat actggtacgg
     3301 tagatatcgt ggcggataaa acgctgtacc agccaggcga aaccgcgaag atgctgatta
     3361 cttttccgga gccaattgat gaagcattat tgacgctgga acgcgatcgc gtggaacagc
     3421 agtcgctgct ttcgcatccg gcaaactggc taacgctaca acgtttaaac gatacccagt
     3481 atgaagcccg ggttccagtg agcaattcct ttgcgcctaa catcactttt tcggtgctgt
     3541 atacccgtaa tggtcagtac agttttcaga acgccgggat caaagttgcc gttcctcagc
     3601 ttgatatccg ggtgaaaacg gacaaaaccc attaccagcc tggtgaactg gtcaatgtcg
     3661 agttaacctc gtcgctgaaa ggtaaacctg tttctgcgca gctaacggtt ggcgtggtcg
     3721 atgaaatgat ctacgcgctg caaccggaaa tcgcgccgaa tatcggcaaa tttttctatc
     3781 cgctggggcg taacaatgta cgcaccagct ccagtctgtc gtttatcagc tacgaccagg
     3841 cactctccag cgagccggtt gcgcctggcg caactaaccg cagcgagcgg cgagtaaaaa
     3901 tgcttgaacg tccacggcgt gaagaggtgg ataccgcggc atggatgccg tcactcacaa
     3961 ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta acccgctggc
     4021 gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc
     4081 gttcggaaaa aaatctctac atgaagtgga gtatgccaac ggtgtattgc gtgggcgaca
     4141 aaccggcggc aggactgttt atcttcagtc agcaggataa cgaaccggtg gcgctggtga
     4201 ctaaatttgc aggcgctgag atgcgccaga cgctgacgct gcacaaaggg gcgaattata
     4261 tttcgctgac gcagaatatt cagcaatctg gcttgttaag tgcagaattg caacaaaatg
     4321 ggcaagtgca ggacagcatt agcacaaaac tgtcttttgt ggacaacagc tggcccgttg
     4381 aacagcagaa aaatgtcatg ctcggcggtg gcgagaacgc gctgatgttg cccgagcagg
     4441 cgagtaatat ccggctacaa agtagtgaaa cgccgcagga gattttccgc aacaatcttg
     4501 atgcgttagt cgatgaaccg tggggtgggg tgatcaacac cggtagccgt ctgatcccgc
     4561 tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac gacattcgtc
     4621 agatgattca ggataaccgt ctgcggctga tgcaactggc ggggcccgga gcgcgcttta
     4681 cctggtgggg tgaagatggc aatggtgacg ccttccttac ggcatgggca tggtacgccg
     4741 actggcaggc cagccaggcg ctcggcgtaa cgcaacaacc ggaatactgg cagcatatgc
     4801 tcgacagtta tgccgagcag gcggataaca tgccgttatt gcatcgggcg ctggtgctgg
     4861 cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt gaaagggttg gatgaagcta
     4921 tcgcccggcg cggaactaaa gatgaagatt tctctgagga agacatccgc gatatcaatg
     4981 acagcctgat cctcgataca cctgaatctc cactggcaga tgcggtggca aacgtcttaa
     5041 ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt gatgccacag gttcagcaat
     5101 atgcgtggga taaagcggca aacagcaatc agccgctggc gcacacggtt gtgctgctca
     5161 atagcggtgg tgacgctacc cagacggccg ctattttaag tggtttgacc gctgagcaat
     5221 ccactattga gcgcgcgctg gccatgaact ggctggcgaa atatatggcg acaatgcctc
     5281 cagttgtttt gcctgcgcct gcgggcgcat gggccaaaca taagttaact ggagggggcg
     5341 aatactggcg ttgggttggt cagggtgtgc cggacattct ctcttttggt gacgaattat
     5401 cgccgcaaaa tgtgcaggtc cgctggcgtg aaccggcaaa aacggctcaa caaagtaaca
     5461 ttccggtaac cgttgaacgc cagttgtatc ggcttatccc cggtgaagaa gagatgagct
     5521 ttactctgca accggtgacc agcaatgaga ttgacagcga tgcgctgtat ctcgatgaaa
     5581 tcacgcttac cagcgagcag gatgcagttc tgcgctatgg tcaggtggaa gtaccgctcc
     5641 cgccgggagc cgacgttgag cgcacaacat ggggcatttc ggtcaataaa cccaacgccg
     5701 cgaaacagca ggggcaattg ctggaaaaag cgcgtaatga aatgggcgaa ctggcctata
     5761 tggtgccggt gaaagaactg acgggaacgg tcactttccg ccatttgctg cgcttctcgc
     5821 aaaaagggca attcgttctg cctcctgctc gttatgtgcg ttcctatgca cctgcgcagc
     5881 aaagtgttgc ggcagggagt gaatggaccg ggatgcaggt gaaataagtg aactggcgca
     5941 gaatcgtctg gctattggcg ttggttactc tgccaacgct tgcggaggag acgccgttac
     6001 aactggtgct gcgcggtgcg cagcacgatc aactgtatca gctgtcgtca tcaggcgtga
     6061 ccaaagtctc agcattacct gactcgctca ccacgccgtt agggagtttg tggaaactgt
     6121 atgtttacgc ctggctggag gatacccatc aaccggagca gccctaccag tgtcgcggaa
     6181 actccccgga agaggtttat tgttgtcagg ctggggagag cataacgcgc gataccgcgc
     6241 tggtacgttc ctgtgggctc tattttgcgc cgcagcggtt gcatattggc gcagatgtgt
     6301 gggggcaata ctggcaacag cgtcaggcac ctgcgtggtt agcgtctctt acaacgctga
     6361 agccagaaac ctcggtaacg gttaaatcgc tgcttgattc attagcgacg ttacctgcgc
     6421 aaaacaaagc tcaggaagtc ttactggatg tggtgctgga cgaggcgaaa atcggtgtcg
     6481 catccatgct cggtagtcgg gtacgggtga aaacctggag ctggtttgcc gatgataaac
     6541 aggaaatccg ctagggtggt tttgctggct ggttaaccga tggtacaccg ctgtgggtta
     6601 ctggcagtgg caccagcaaa acggtactaa cccgttatgc gacggtgctt aatcgggttt
     6661 tgccagtgcc gacgcaggtt gccagtgggc aatgtgtaga ggtggagctg tttgcccgct
     6721 atccgctgaa gaaaatcaca gcggaaaaga gtacgacggc agtaaaaccc gacgtactta
     6781 atgggcgcta tcgtgtgacg tttactaatg gtaaccacat tacgtttgtc agccacggtg
     6841 agacgacctt attgagcgaa aagggcaaac tgaaattgca gtcgcatctc gatcgtgaag
     6901 agtacgttgc cagagttctg gatcgcgagg cgaaaagtac gccacctgag gcagcgaaag
     6961 ccatgaccgt ggcgatacgt acttttttac agcaaaacgc caatcgggaa ggtgattgcc
     7021 tgaccatccc cgacagcagc gccacgcagc gtgtttctgc ttcgccagcg accacggggg
     7081 cgcgaacgat gaccgcctgg acgcaggatc tcatctatgc aggcgatccg gttcattatc
     7141 atggcagtcg cgctaccgaa gggacacttt cctggcggca agccacggcc caggcggggc
     7201 agggggagcg ttacgaccag atactcgcgt ttgcttatcc cgacaacagc cttagccgct
     7261 ggggagctcc acgctcaacc tgccagttat tacccaaagc gaaagcctgg ctggcgaaaa
     7321 aaatgccgca gtggcggcgt atattacaag gcgagacggg gtacaacgaa ccagacgtgt
     7381 ttgcggtctg tcgtttagtc tcaggtttcc cctataccga tcggcagcag aaacggctgt
     7441 ttattcgtaa cttctttacg cttcaggatc ggctggattt aacccatgag tatctgcacc
     7501 tggcctttga tggttatccc accgggcttg atgagaacta tatcgaaacg ctgacccgac
     7561 aattattaat ggactaatgc tatgcgaaag atttttcttc cgttgttact ggtggcactt
     7621 tcgcctgtcg ctcacagtga aggtgtgcag gaagtcgaga ttgacgcacc gctctctggc
     7681 tggcatccgg cagagggcga agatgccagt ttttcacaat cgatcaatta cccggcgtcg
     7741 tccgtcaaca tggcagatga tcaaaatatc tcagcgcaga tccgcggcaa aataaagaat
     7801 tatgctgcgg cgggtaaagt tcagcagggc cggctggtgg tcaacggtgc cagtatgccg
     7861 cagagaattg aatccgatgg ttcatttgca cgcccttata ttttcactga aggcagcaac
     7921 agcgtgcagg tcatcagccc ggatgggcaa agccggcaaa aaatgcagtt ttactcaacg
     7981 ccgggtacag gaacgattcg tgcacgttta cggctggttc tctcgtggga tacggacaat
     8041 accgatctcg atctccatgt tgtcacgccc gatggcgaac acgcctggta cggtaacacc
     8101 gtgctgaaaa acagtggtgc actggatatg gatgtcacga cggggtacgg acccgagatt
     8161 ttcgccatgc cagcaccagt tcacgggcgc tatcaggtgt atatcaacta ctatggtggg
     8221 cgcagcgaaa cggaattaac caccgcccaa ctgacgctga tcaccgatga aggatcggtc
     8281 aatgagaaac aggaaacatt tattgtaccg atgcgtaatg ctggcgagct gacgctggtg
     8341 aaaagttttg actggtgatc catcccttca cacaacctgg ctctctcttt tttcagataa
     8401 gactgctggg cgatctgccg acgccaggat ataaccatta acaagcgtcg gcagattggt
     8461 catttcttat ttttcgtact gagaactgtc catctactcc ggctttcctg gttaaggcaa
     8521 tggtttctaa gcgatacgca ttattgcaat ggtgacagaa cattgcggta acacgctttt
     8581 accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc ctttgaaata
     8641 ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag cattggcgtt
     8701 agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg cgttacatca
     8761 gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc agcgctttat
     8821 tcaacgcacc tctgatgtga tgaaagagct gaagtacatc gccgaaaatc gcttatcggc
     8881 agaaaacggt gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc ctgcgtttga
     8941 accgctgttt gccgactccg attgttccgc aatgagtaac acctggcgag gttctctgga
     9001 gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt acgatctcaa
     9061 ccgggtattt ttaatcggca gtgataacct ctgcatggcc aatttcggtc tgcgtgatat
     9121 gccagtggaa cgcgataccg cgttgaaagc tttgcatgaa cgcatcaata aatatcgaaa
     9181 tgcaccacaa gatgatagcg gcagtaacct ctactggatc agcgaaggtc cgcgccctgg
     9241 cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg aaccggttgc aggcgctttt
     9301 gggtgtcgag cagaccatcc ggatggagaa ctttttctta ccgggtacgt tgccgatggg
     9361 ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac cagaaagtaa
     9421 aattaagggc gatcctcgct ggatgcagga acgctcctgg tttggctata cggaagggtt
     9481 ccgggagctg gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg tgtattcggt
     9541 gccggttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa ttttgctgaa
     9601 tgtgcttgcc ggagctgcat tgtttactct cgcacggatg tacgagcgac gtattttcat
     9661 tccggcggaa agcgacgccc tgcgactgga agaacatgag cagttcaatc gcaagattgt
     9721 cgcctccgca ccagtgggta tctgcatttt gcgtaccgct gatggcgtca atattttaag
     9781 taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc aacgactgac
     9841 gcaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca acaataccaa
     9901 tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct
     9961 ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg cacaagcagc
    10021 ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac
    10081 gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt taccgaaagg
    10141 cgtcgatcgg ctggtgacgg caatgaacaa ctcttccagc ctgttgttga aaattatcag
    10201 cgatattctc gatttctcga agattgaatc ggaacagttg aagatcgaac cgcgtgagtt
    10261 ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg tggtacgcaa
    10321 gcagttaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa atggcgaccc
    10381 gatgcgttta cagcaggtca tctccaacct gttgagtaac gccataaaat tcaccgatac
    10441 cggctgtata gttttgcatg tccgcgctga tggcgattat ctctctatcc gtgttcgcga
    10501 taccggcgtg gggattccgg cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt
    10561 cggaacgggc gtacagcgaa acttccaggg gaccggtctg ggtctggcga tttgtgaaaa
    10621 actgatcagc atgatggacg gcgatatctc ggtagattca gaaccgggaa tgggcagcca
    10681 gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg
    10741 gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga
    10801 aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg aaccgactcc
    10861 cgaagatgtg ttaattactg acgaggtagt gagtaaaaaa tggcagggta gagcggtagt
    10921 gaccttctgt cgtcgccata ttggtattcc gctggagaaa gcgccagggg agtgggtaca
    10981 cagtgtggct gccccgcatg agctaccggc attgttggcg cgtatttatt tgatcgagat
    11041 ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca gcgataatga
    11101 cgatatgatg attctggtcg tggatgatca tccgattaac cgacgtctgc tggcagatca
    11161 gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggtgtcgatg cgcttaatgt
    11221 acttagcaag aatcatattg atatcgtgct tagcgacgtc aacatgccaa atatggatgg
    11281 ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac
    11341 tgctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg acagctgcct
    11401 gtcgaagccg gtaacgctgg atgtgataaa acagacgctg acggtatatg ccgagagggt
    11461 caggaaatcg cgggaatcgt aggacggata aggtgtttat gtcgcatctg gcattcagtg
    11521 caaatgccag atgcgacgct gacgcgtctt atctggccta ctttgatgta atctgttgaa
    11581 ataatgggaa tcgtaggacg cataaggcgt ttacgccgca tccggcaatc ggtgcaaatg
    11641 ccagataaga cactaacgcg tcttatctgg cctacaggtg attagtcttt atctgccgga
    11701 cttaacgtca ctgaagagag ataattcagc agggcgatat cgttctcgac acccagcttc
    11761 atcatcgcag atttcttctg gctactgatg gttttaatac tgcggttcag ctttttagcg
    11821 atctcggtca ccaggaagcc ttccgcaaac atgcgcagaa cttcactctc ttttggcgag
    11881 agacgcttgt caccgtaacc accagcactg attttttcca acaggcgaga aacgctttcc
    11941 ggggtaaatt ttttcccttt ctgcagcgcg gcgagagctt tcggcagatc ggtcggtgca
    12001 ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag aatcgccggg
    12061 ttgttgttca tggtcagaac aatgattgac aggcttggga aatggcgctt gatgtacttg
    12121 attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt aatcaacaca
    12181 tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa ttcgccgaca
    12241 acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac tatcggatgg
    12301 tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct acagcaagct
    12361 cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat ccttctcacg
    12421 aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta gcatggcaaa
    12481 tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg ttgctgcttc
    12541 agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat aatagccgct
    12601 ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct cttcctgcgc
    12661 cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca tattgaagtt
    12721 gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc ttaaaagcaa
    12781 gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat cttgactaat
    12841 taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc gagtgacaat
    12901 attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca gtaaacgctc
    12961 ttcttcctct tcaactttcg ggtcagcggc gagcattttg atatgcacag agtagcgtgt
    13021 accaagccca tcccgcgttt tgatgtttaa atgaccgccc agtttacgtg ccagttgatc
    13081 gctcagccag aatgccagcg ggtccgcctt gccatagcga tcgttttggg tctggttgat
    13141 aaacgggaag tgcaaattat ccatttcatg aatgcttacg ccttctcccg tgtccagaat
    13201 gcggaatgtc aggcggtctt cggaggactc atcctgatca acctcaaggg tgatttttcc
    13261 caattgcgtt gaggtcacgg catattgcat cagtagtagc aaaatacgtc gtaaggcatc
    13321 gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg ttaatcagca gttgcagacc
    13381 tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaat cctgcacgga
    13441 gaacagcacc gtctcacttt tccagctatc gtccgcaagc atgttcgcta actgtatttc
    13501 atcgaccagc cgcaccagca catctgcctg attcgccagt tgtttgcttt ccggggcatt
    13561 gagtttagcc gcgctctccg ccagggactg tgcgggttct ttcagcgcat cgccaatgtt
    13621 tttcataaag gtcatccgcc cctgctggtt tttctcatac agacgctgcg cctgcttgag
    13681 tttcttgttt accagcactt cgcgatcctg atcgcgaata atgaaaattt gtgtgcgcgg
    13741 cgcgacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg tcgcctgaat
    13801 aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat gcggcagcaa
    13861 atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt gaaccagcag
    13921 gccgagcggc agcagtgaga ctatctcttc attaattgcc cgtaaaatgc gcaattcgtt
    13981 attgaccgcc gtgctgggta gactttctgt actgcggctg gagaaatggc ggaatgtggt
    14041 atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca gcagaatgtt
    14101 ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc gcatatcggt
    14161 agagttgagt gccgaggaga tttcaatctt cgtactgtta aagtggatac tgacgctatc
    14221 cgtcccttct ttctcattat cattgtctcc cgttgccgtc gcgtctggct caaggcggaa
    14281 actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa aagccacgac
    14341 cgttgccaga tgtcccggct ggttgaacgt ggtacgcaag gtaaagtaat gaccgttctg
    14401 ccaggccagg cggcgcatgt tagaaaaact ttcgcgttca tcgagggcgt tggcctgttg
    14461 caacatttct gcacgacgtg aatcaacaat gtcactgacg gtcgattctt taaatccgga
    14521 ggtgagatct ttgagggaca gggttgagat cagcaccaga ctgttatcct gaccattcag
    14581 gtaatacatt gaccacggta cattttctgc gccccacaat gtgtccagat aggtggacat
    14641 ccgttgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg cttccgtttt
    14701 gcggcgcggt ttttccagat agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc
    14761 gccggaggag ggggaggtcg tcgcggcaat attgtcgtag atctgccagg tcacgtaacg
    14821 ccaggtatcg acgcgctttt ggatagcgtg ggtaatgtcg acaatctggt aacttttatc
    14881 tttcagccag gcgttaacgg cgctttgtac cattacaccc atcgtcacca gtaacacaat
    14941 gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc gggtcgtggc
    15001 cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg aagtgatagg
    15061 gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga ttctctattc
    15121 tcccgtgata tttatcatcc tgcattgggg aaaatagatg tacaagcgcc attttgctgt
    15181 acaaataacc tacgaaaagc ccaacgaaat taattgcaaa taaacgaaaa ttgctggaaa
    15241 ttatgctgat gttaattatt tgtgaaatag ttaacaagcg ttatagtttt tctctggtag
    15301 cacagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga ggcatccggt
    15361 tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa gttaatgatg
    15421 atagcgggag ttattctagt tgcgagtgaa ggttttgttt tgacattcag tgctgtcaaa
    15481 tacttaagaa taagttattg attttaacct tgaattatta ttgcttgatg ttaggtgctt
    15541 atttcgccat tccgc
//
LOCUS       DAJSKJ010000085        15550 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000085 DAJSKJ010000000
VERSION     DAJSKJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.085"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            51..617
                     /locus_tag="ON345_002316"
     CDS             51..617
                     /locus_tag="ON345_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR3072369.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            621..1196
                     /locus_tag="ON345_002317"
     CDS             621..1196
                     /locus_tag="ON345_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR3072370.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1238..2290)
                     /locus_tag="ON345_002318"
     CDS             complement(1238..2290)
                     /locus_tag="ON345_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR3072371.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLLVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2515..3435
                     /gene="lpxL"
                     /locus_tag="ON345_002319"
     CDS             2515..3435
                     /gene="lpxL"
                     /locus_tag="ON345_002319"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR3072372.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGFGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3607..4833
                     /gene="mdtG"
                     /locus_tag="ON345_002320"
     CDS             3607..4833
                     /gene="mdtG"
                     /locus_tag="ON345_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HCR3072373.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTMLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4916..5290
                     /locus_tag="ON345_002321"
     CDS             4916..5290
                     /locus_tag="ON345_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR3072374.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5291..5518)
                     /locus_tag="ON345_002322"
     CDS             complement(5291..5518)
                     /locus_tag="ON345_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR3072375.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5691..8234)
                     /gene="mdoH"
                     /locus_tag="ON345_002323"
     CDS             complement(5691..8234)
                     /gene="mdoH"
                     /locus_tag="ON345_002323"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR3072376.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8227..9762)
                     /gene="mdoG"
                     /locus_tag="ON345_002324"
     CDS             complement(8227..9762)
                     /gene="mdoG"
                     /locus_tag="ON345_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR3072377.1"
                     /translation="MMKMPWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLSFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10156..11313
                     /gene="mdoC"
                     /locus_tag="ON345_002325"
     CDS             10156..11313
                     /gene="mdoC"
                     /locus_tag="ON345_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR3072378.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11321..12802)
                     /gene="clsC"
                     /locus_tag="ON345_002326"
     CDS             complement(11321..12802)
                     /gene="clsC"
                     /locus_tag="ON345_002326"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR3072379.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12744..13277)
                     /gene="ymdB"
                     /locus_tag="ON345_002327"
     CDS             complement(12744..13277)
                     /gene="ymdB"
                     /locus_tag="ON345_002327"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR3072380.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13372..13683)
                     /locus_tag="ON345_002328"
     CDS             complement(13372..13683)
                     /locus_tag="ON345_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR3072381.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13804..14136)
                     /gene="csgC"
                     /locus_tag="ON345_002329"
     CDS             complement(13804..14136)
                     /gene="csgC"
                     /locus_tag="ON345_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR3072382.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
     gene            complement(14195..14650)
                     /gene="csgA"
                     /locus_tag="ON345_002330"
     CDS             complement(14195..14650)
                     /gene="csgA"
                     /locus_tag="ON345_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR3072383.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14691..15146)
                     /gene="csgB"
                     /locus_tag="ON345_002331"
     CDS             complement(14691..15146)
                     /gene="csgB"
                     /locus_tag="ON345_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR3072384.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3878 a         3992 c         3689 g         3991 t
ORIGIN      
        1 tctccgacac gccattttct ttctctcgca aattcgtatt ggatgcactt atgtcattca
       61 caaatacccc tgaacgctat ggtgttatat ctgcggcctt ccattggcta agcgcaatta
      121 ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat tacgatggct
      181 ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat tttgttaatg atggggctgg
      241 ttattcgcgt tctgtggcgt gtcatatctc caccgccggg tccgctgccg agttattcgc
      301 ccatgactcg tcttgccgca aaggctggac atctcgcatt gtacctattg ctgttcgcca
      361 tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc gtttttggct
      421 ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca ggcagacttt gccggtgccc
      481 tgcatttttg gcttgcgtgg agcgtcgtag tactgtccgt tatgcacgga tttatggccc
      541 tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga aagtcgtcat
      601 ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga
      661 tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa ggtcagcacg
      721 cctttgttaa tttccgcatc cagcaccttg gctatagctg gttatacggc acctttaaag
      781 atttcgacgg tacttttacc tttgacgaaa aaaatccggc tgccgataaa gtgaatgtga
      841 caattaacac caccagcgtc gatactaatc acgccgaacg cgataaacat cttcgcagtg
      901 cagatttcct caataccgca aaatatccac aggcaacatt cacctccacc agcgtgaaga
      961 aagacggtga cgaactggat attaccggcg atctgacgct gaatggcgta accaaacccg
     1021 tcacgctgga agcgaaatta attggtcagg gcgacgaccc atggggtggt aaacgtgcag
     1081 gcttcgaggc cgaaggcaaa attaagctca aggacttcaa tatcaagaca gatttaggtc
     1141 cagcttctca ggaagtggat ctgattattt cagtggaagg cgtacagcag aagtaagagt
     1201 gaaatgttgc cggatggcac gcatccggca atgatattta ttccgttgga tcaggaatgc
     1261 ccagtgttgt attcagacgt ccacgagact tattaaagat cttattgcca ttttcacgtc
     1321 ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag caaatctcac
     1381 tacaacaacc tttgtatttt tccgcacata ctggacactg aataaacagc aggtggcagc
     1441 catcattttt acagttggta tggctgtcgc acggcgcacc gcactggtgg caatgcgcga
     1501 taatctcatc agatatacgt tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa
     1561 aacgcaccag caagccctgc tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga
     1621 tatgccagac tttgttgaat ccgttatgtt tcatccaggc actggccttt tcacaacgaa
     1681 tgccgccggt gcagtacatg acgatttttt tatctttatg tgcctgcatc atctcgactg
     1741 cttttggcag ctgctcacgg aaggtatctg ccggaatttc gagcgcgttt tcaaagtgcc
     1801 ccacttcata ctcatagtgg ttacgcatgt cgataaatag tgcatcggga tcgtcaagca
     1861 tggcgttcac ttccgccgct tgcagatact caccaacatt gctggcatca aagtgaggat
     1921 cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat gcgcagtacc cagaaggatt
     1981 tcccgtcatc atccaacgcg atattcaggc gtaaaccctc taaagccggg tcgaaggcat
     2041 agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc
     2101 cctcatgcgc cagatacact cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag
     2161 cgtcacgggt cgccttagga tcggcgatgt ggaaatactt gtaaaacgaa atggtggttc
     2221 gcggttcgct ctcagccaac attttggctt ttagcgcgtc gttggaaatg cggttgtgta
     2281 acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg gcatgatata
     2341 gcaattatca ataattaaca tccacacatt ttacgctaca tttgcgcatt aaaaattatt
     2401 tgttatttac aagcgcggca atttcgccca gtcttcagcc acaaattttg gttgcgggcg
     2461 aaaaaatgcg acaatacata caattgcccg aataggttga aaaacaggat tgatatgacg
     2521 aatctaccca agttctccac cgcactgctt catccgcgtt attggttaac ctggttgggt
     2581 attggcgtac tttggttagt cgtgcaattg ccctacccgg ttatctaccg cctcggtttt
     2641 ggattaggaa aactggcgtt acgttttatg aaacgacgcg caaaaattgt gcatcgcaac
     2701 ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat
     2761 ttcgaatccg ttggcatggg cctgatggaa accggcatgg cgtggttctg gccggaccgc
     2821 cgaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa
     2881 aaacgcggca tcctgttagt tggcatccat tttctgacac tggagctggg tgcgcggcag
     2941 tttggtatgc aggaaccggg tattggcgtt tatcgcccga acgataatcc actgattgac
     3001 tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg caaagattta
     3061 aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat
     3121 tacggcccgc gctcaagcgt tttcgtcccg ttgtttgccg ttgagcaggc tgcgaccacg
     3181 accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc
     3241 cgtaagccag atggcaaagg gtatcaattg attatgctgc cgccagagtg ttctccgcca
     3301 ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg
     3361 atggcaccag agcagtatat gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt
     3421 ccttcacgct attaaatctc ccatgccgga tgcttcagaa tggcatccgg cattaccaca
     3481 gcaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg aagtcgggcg
     3541 cataattagt gtgcttatct tttcttctta tgttcaccgc gcctggcgca ccaacagcgg
     3601 attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa cctgatcgtc
     3661 gcctggctag gctgttttct taccggggcc gccttcagtc tggtaatgcc cttcttaccc
     3721 ctctacgttg agcagcttgg cgttaccggc cactccgccc tgaatatgtg gtccggtatt
     3781 gtcttcagca ttacattttt attttcggcc atcgcctcac cgttttgggg tggactcgcc
     3841 gaccgtaaag gccgaaaact catgctatta cgctctgctc tcggcatggg catcgtgatg
     3901 gtgttgatgg gactggcaca aaatatctgg cagtttttga tcctacgggc gcttcttggg
     3961 ttacttggcg gatttgtccc caacgctaat gctcttatcg ccacacaagt accgcgtaat
     4021 aaaagcggct gggcgctggg tacgctctcc acaggcggcg ttagtggtgc gttgctcggc
     4081 ccaatggctg gcggcctgct cgccgatagc tacggcttac gtccggtatt ctttattacc
     4141 gccagtgtgc tcatactctg ctttttcgtc accctgttct gcatcagaga aaaattccag
     4201 ccggtcagca aaaaagagat gctgcacatg cgggaagtgg tgacatcact taaaaacccg
     4261 aaactggtac tcagcctgtt tgtcaccatg ttaatcatcc aggtggcgac aggctcaatt
     4321 gcccccattc tgacgctgta tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt
     4381 atcagtggca tgatcgcctc ggtgccaggc gtggcggctc tgctaagcgc accacgactc
     4441 ggcaaacttg gcgatcgaat cggacccgaa aagatcctga ttacagcgct gatcttttct
     4501 gtactgctgt tgatcccaat gtcttacgtt cagacgccat tgcaacttgg gattttacgt
     4561 tttttgctcg gtgccgccga tggtgcacta ctccccgccg tacagacact gttggtttac
     4621 aactcgagca accagatcgc cgggcgtatc ttcagctata accaatcgtt tcgtgatatt
     4681 ggcaacgtta ccggaccatt gatgggagca gcgatttcag cgaactacgg tttcagagcg
     4741 gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag tctattcatg gaacagtcta
     4801 cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc
     4861 ggagaatcag ctatcctttt ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac
     4921 catgtacgca acgcttgaag aagccattga cgctgcacgc gaagaatttc ttgcagacaa
     4981 ccccggcatc gacgccgaaa atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt
     5041 gcaggacggc gacatcatgt ggcaagttga gttttttgcc gacgaagggg aagaaggtga
     5101 atgtttacct atgcttagcg gtgaagccgc gcaaagtgtt tttgatggcg actatgatga
     5161 gatagagata cgccaggagt ggcaggaaga gaatacatta catgaatggg acgaggggga
     5221 atttcagctt gagccaccgc tggataccga ggaaggacgc gcagcagctg atgagtggga
     5281 tgaacgttaa tcactcatac gggccatgat gaatgtcgat cggcagcagt aaagtatcaa
     5341 aaaccagaga gaatggcaga tcaaggatcg tgacataacg ccaggcggag tcacgcacat
     5401 cccattgcac accgggataa tattggttgc catgcccctg ccccggtata gtgcgactaa
     5461 taatgctgcc acagccactc agcaaggtca ccatgatgct caccacaatt aaacgcattt
     5521 gatacctctt tcgtaaaaaa ataccggcat aacgccggta tttttattgg ctcgttgttt
     5581 ttgccggatg cggcgtgaac gccttatccg acccacaaaa tcttgcaaaa tcaataaatt
     5641 gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc aactacgttt ttattgcgaa
     5701 gccgcatccg gtttacgcaa tgccagtgga ttgagcttta tcccttcgta ataactcacc
     5761 catgaagaat atctctccgg ggaattccag acgcggaaat gcagacgggc catcgtcacc
     5821 ggatcgctta gcagcaccag gcgacgatcg cgattcagct tctctggcgt ctcgttcagc
     5881 gcctgttcaa cgtggcggtc acgggcgatt tccagcacct tactggcgcg gtgacgcgcg
     5941 gtcgccattg cggttgccag agcgttaaat gacgggttaa acactgcgtg cataaagcca
     6001 tcatcaaggg agcgttgacg attcatctca aggaaccgat cggtatcaac cagcacctgc
     6061 ggcggcgaat actcttccgg gatcaggaac agtttccagc gtttggtgcg cagaccaacg
     6121 gtggcacggc tggaaatcac cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt
     6181 gccagccaga acaggaaacg cagatccaac cacgccatac caacagccca cactaaccct
     6241 aacagcagct gtgagccgtg gcgtttgaac gcttcacccc aggaagtgga gtcatcatca
     6301 cgctgcggtg aattccacac cacttcccag ccaaggaacg cgctgacgac gaagaccgta
     6361 tggaacagca tgcgtaccgg agccagcagc acggaaaaca gcacttccag cagcaacgat
     6421 aatgtaacgc gccagaagcc gccgtattct ttcgttcctt tgcaccagat aagcaaaatg
     6481 ctcaataact tcggcaggaa caacagcacc atggtcgaag caaaaagtgc aatcgccagc
     6541 tcaggacgcc actgcggcca caccgggaac aactgccgtg gttgcaggaa gtattgcggt
     6601 tcggtcaacg catgtactac ctgcaatgca gtagagagcg cgaggaacat aaaccacagc
     6661 ggagcggaga gataagacat cacgcccgtc aggaacaccg cacggtgaac cgggtgcata
     6721 cccttcacca ggaacagacg gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt
     6781 tttagctcat caagcaagtt aggcggcaat tcttcataag aacccgggag atcgtaagca
     6841 atccagaccc cccaacctgc acggcgcatc aacgccgctt ccacgaagtc atgtgacagg
     6901 attgaaccgg caaaggaacc ttcgcccggc agcggagcca gtgcgcagtg ctcgataaac
     6961 ggtttcacgc ggataatcgc gttatgtccc cagtagtgcg actcgccaag ttgccagaag
     7021 tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg cgaactgctg acagcgcgca
     7081 tacagcgtat ccataccgga cgctttcggc gacgactgaa tgatcccggc gttcgggttg
     7141 gcttccatca ggcgcaccag cccgcacaaa caatcaccgg tcattaccga gtcagcatcc
     7201 agcaccacca tgtagctgta ctggctgccc cagcgacggc agaagtcatc gatattaccg
     7261 cttttacgct tcacgcggcg acggcggcgg cgatagaaaa tctgaccttc gccaccgact
     7321 tcagcgataa gctccatcca ggctttttgc tctgcgacgc agatatccgg gttataactg
     7381 tcactaagaa tgtagacatc aaagtgtttg gcattcccgg tggcttttac tgattcccac
     7441 gttgcacgca ggccagcaaa aacacggttc acgtcttcgt tacagatagg catgatcaac
     7501 gccgtgcgat gctccgggtt taatggttca tcgccaactg ttgacgcaga tatactgtat
     7561 ttatcgcgac caataagcag ttgcaggaag cccattaacg ccgtccagaa tccggcggac
     7621 acccaacaga acagtaccgc aaagaggatc aggataccgg tttgcagcat ataaggcaga
     7681 agctgcataa aggaaaccca caaatcctga ccaaccatat ccataggatt aatcagcgcc
     7741 caaccctgat aaggaagaat ggtcttcata taccaagtcg cgacgacagt ttgcgcgagc
     7801 gtcaggatca acagaatgta acggcggatg gtaccgacgg tacgccactt ttgctcgctc
     7861 tcctgctctt ctttggtcaa acgagccaga tagcgcggcg tgacatcgcg tccacgcagg
     7921 cgatcccaga aacggcctac cgggttggta cgccacgggt cgggaaacat cgaggagcgt
     7981 tttgcttctg gcatcgcctt cagctgatcg cgcccttcgt cgtctttaat taactgtcca
     8041 tcagcaagtg aatctggcca ggcttgttcc agacgcgcct ttaccgagcc ttgcggggaa
     8101 tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg cctgatgaac ggcgcggata
     8161 tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg gcattgcgtc aatgtactca
     8221 gttgtcttat tcattggcag gtaactggta gctccaggtt tcactcaacg tctgatcggc
     8281 attcaccagc gcagcacgca tttcagtggt tttcttggca tctttcactt tcacacgcat
     8341 caccagacgc cagcctttgg taaccgggtt atagcgcacc gtgctttcaa ctatctcacc
     8401 attatcacca atgctggttt gcgctgtgac cggggtatcc tctggcagtt ttttcatctc
     8461 tgcgccggta aaatcgacca caaaggcgat agtaccgtca ggctggcgaa tcaggttcga
     8521 ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg
     8581 cagtttgtct tcatcacggc tgaaggtgat ggtgtattta aagttcatct ctttacccgg
     8641 ctccggcagc tgatccggcg tccagtaagc gacgatgtta tcgttggttt catcgttggt
     8701 tggaatttcc accagctcaa cgctgccttt accccactcc cctttcggag tcacccatgc
     8761 gcttggacga agatcgtaac gatcatcgag atcttcaaag cgggagaaat cacgaccgcg
     8821 ctgcaacaga ccgaagcctt gcgggttctc catcgagaag ctgctgaccg ctaaatgttt
     8881 cgggttattc aacggacgcc agatccattc gccattaccg gcatggatag acagaccgtt
     8941 agagtcgtgc aactccggac gatagttatt tgcaggcgac ggttggttcg gcccaaacag
     9001 gaacatactg gttaacggtg caacccccag tttgccgact ttatcgcgca gatagatttt
     9061 cgactgcaca tccacaaccg tgtcacgccc tggcataacc acgaatttgt aagcacctgt
     9121 cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt ttatcagtcg gttttggacg
     9181 ctcgatccag aactctttga agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat
     9241 tgccaggccg cgggcagaaa ggccataaac ctgacctgca ccaatcacgc ggaaatagct
     9301 ggccccgagc atgctgacga tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac
     9361 tttaaaaccg gcaaaactaa ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa
     9421 agtgaaataa tccgggctgt atttgattcg tttgactgcg gtggcagtca cttcatttat
     9481 tttgaccggg gtatcgaagt acataccctg atggtagaac tcgagtttga atggggtctt
     9541 cagattgttc cagtacgctt tgtcatgatt aaactggatc tgctgatagt ccgcgtattt
     9601 catatcgcgg aaaacggagg gcaagttgct tttgggcgcc tcatagcctt tcccggctaa
     9661 ggattgagct tgctttgcga catcatcaat actgaaagcc cagcttgaag atgtatacag
     9721 ggttaacatt actgcagcac tcaaccaagg cattttcatc atttgtagtt tatgtttcat
     9781 aattagtaag cacttccccc tttgtgtgct tatatcgatc cgatctattt taatggataa
     9841 ccagccattc cgacaacaga atcccggtat cgttcagcac gctggctcat ggattaagca
     9901 cctgattgca accgttttca ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt
     9961 gtagggtttg ccagcaattt tatttatcaa ccgtcttaca gacgcttcca ttctggttag
    10021 tggtagcgat gatgctatgc ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg
    10081 tgtcgctatg gcaacaagcc taaaggcata acccgacagg gataagacga aaaatccgag
    10141 attagttaac catatatgaa tccagtaccc gcgcaacgtg aatatttcct cgactccatc
    10201 cgcgcctggc tgatgttgtt agggatccct tttcatattt ctttaatcta ttcgagccat
    10261 acatggcatg tgaatagcgc cgaaccgtca ttgtggctga ccctttttaa tgacttcatc
    10321 cactcgttcc gcatgcaggt atttttcgtt atatccggct acttttccta catgcttttt
    10381 ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg
    10441 ttaacagcca tccccttact gacattgccg caatttatta tgctgcaata cgtcaaaggg
    10501 aaagcggaaa gttggcctgg gctgtcattg tatgacaaat ataatacgtt ggcctgggaa
    10561 ttaatatcac acctgtggtt tttactggtg ttagtagtca tgacgacgct gtgcgtatgg
    10621 atatttaaac gcatcagaaa taatttagaa aattctgata aaacgaataa aaaattctcg
    10681 atggtaaaac tatcggtgat ttttttatgc ctcggcatcg gctatgcggt aataagaaga
    10741 acgattttta ttgtgtatcc gcccattctg agtaatggca tgttcaattt tattgtcatg
    10801 caaacgctat tttatttgcc gttctttatc ctcggtgcac tggctttcat tttccctcat
    10861 cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc ttgcagcagc attggcattt
    10921 gtcgcttatt tactcaacca gcgctatggc agtggcgatg cctggatgta cgaaaccgag
    10981 tcggtgatca ccatggtcct cggtctgtgg atggtgaatg tggtcttctc ctttggccac
    11041 cgtttgctta acttccagtc agcgcgggtg acttactttg ttaacgcatc gctatttatc
    11101 tatctggttc accacccgtt aacgctgttt ttcggcgcat acattacacc gcacatcacc
    11161 tccaactggc ttggttttct ctgtggcctg atattcgtag tagggattgc gataattctg
    11221 tatgaaattc atttacgcat cccgttactg aagtttttgt tttctggtaa accggttgtt
    11281 aagcgtgaga acgataaagc accagcccgt taagccacat ttacaataac cattccacgg
    11341 gcaatatcga cgccagtctg accataaccc gcttccagaa actggtggcg ggttcttttt
    11401 tgagaataat ctctttcttt gcatgacgat caacccagtt gatccgtccc cacctgtcca
    11461 gacggagctg ccaggccgca tcatactggc tctgaataaa gcgtttatca attaactgtg
    11521 ccagcgtttc gctctctatc acgaagccca tttcagtatt gagcaatgtt gaacgcggat
    11581 cgaaattgaa agagccgata aacaccgttt taccatcgat gctaaaagtt ttagcatgca
    11641 ggctggctcc ggaattaccg gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg
    11701 tcggcttgag ttcatataat tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt
    11761 atccggcatg gacgacagca acatcgttag cggcaagaga attggttagg atcgcaatct
    11821 ttaccccttt tctcaccatc cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat
    11881 aggaagagat aatatcgatg cgttcactgg gtgagcccat gatatcgaac aggcgctgcg
    11941 gtaacagtga atgccgtttt gccttgccct cccctttcgc cggatcatca cttaataaac
    12001 gtgttttcgc ccagataagc ggcaacgttc catcaaccag atgatttata aacggactgg
    12061 attccatttg gcgtaagtaa cgatgcgtca tggcatcgtt atgccaggag gcgggtaact
    12121 cgatgcgatc cgccatttca ccttccggga catccagcac ctgctgtaag ggtgaaaccg
    12181 atttgcaata ccagtagcgg gcgaaatcat cggcaacgtc ctctaccacg ggtcctattg
    12241 ccataacatc taaatccgaa aaaagtggct cttcccctgc cccaaaatag gcatcaccaa
    12301 tatttcgtcc tcccaccagg gtaaccacgc catcgacagt gaaactttta ttgtgcatac
    12361 ggcgattgag acgggaaaag tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga
    12421 aaggattaaa aagccggact tcaatgcggg gatggctgtc aagcaagcgt aaaatatcgt
    12481 caagtccggg cgtattgttg tcgtccagca acaaacggac gcgaacgcca cgctttgctg
    12541 cggctaacag ggcggaaaac agtaaccgac ccgacatatc gtcctgccag atgtaatact
    12601 gaacatccag cgtatgctct gccatttcgg ccagacgata ccgggcggca aacgcatcca
    12661 gacttttctc cagagggaaa agaccacact gaccgggatg ttgcgaacac agtggcagca
    12721 ccgcgctcgc cagccggggc aaatcattca tctccttgtt gggtaaggag tctttcgtag
    12781 aggtgggcgt tttcttcatc ataacagaca aagtatacct gttcgggtaa agcgtgacgg
    12841 gtaataaatt ctgaaacggt tttaactgcg atttcagccg ctgccgcacg agggtaacca
    12901 taaaccccag tactgattgc aggaaaagcc actgacgtat agctatttgc cgccaccagt
    12961 cgcaggctat tcagataggc atcctgcaaa agctggtctt cgttttgttc accaccacgc
    13021 cagactggcc cgacggtgtg tactacggct ttagcgggaa gatcgcctgc aagcgtaata
    13081 acggcatgcc ccgtagggca atcgccctgc tgttgcctga cttttaaaca ggcatccagc
    13141 agggccggac ccgctgcgcg atgaatggcc ccatcgacgc cgccgcctcc cattaatgac
    13201 ggattagccg cattcacaat cacatcaacg gccagtttgg taatatcacc ctgcacaaca
    13261 tgaatacgcg ttttcataac gcctccttac accttcttgt ttcttaagtg tacagcaggc
    13321 tttttggtta aatctcattt ttctgctcag caacgtgcgt tgttcagaaa cttagttata
    13381 ttcgatgttc attacagcca gctttttctg tttatcaaga tactgcactt ttactgaggc
    13441 tatttgcgcg tttttgacat tgccgctcat taacgctttg ctggaatagg ttttgcccgg
    13501 aacagaacca ttctgtgggc agttcatttc tacgggtgat gactgggtgc tgacgtcaca
    13561 tgccggttcc acaatttggc cataaaaatg gatcacaccc ccttgcgcgg tgcttcctgc
    13621 gatggcaata aaaggaaaaa acaaactgcc gagcataaga gagtttaaaa aaggtctgaa
    13681 catttattct atttcctcaa tgattagtca tccttgaggg ttgtgttatc catactttca
    13741 ggttggctga ttcgcagcag accattctct ccagattcat cttatgctcg atatttcaac
    13801 aaattaagac ttttctgaag agggcggcca ttgttgtgat aaatgaagtg cctgtccatc
    13861 agaaacagta acaactattt tcacccgatc ttccggggaa atatttaaac tcaacttcgt
    13921 caaagcaata ggttgattag caggcaatga gagggtcttt tcttgcttcg tctgactttg
    13981 ccctgaactg ccttcgcgca gggacaatat ttgtactctg cacagacaag attgagtaag
    14041 agtgacttca ggaataatgg tatacacatc cccttgctgg gtcgtattaa aggttatctg
    14101 gctggaaagt gccgcaagga gtaataacgc attcatattc ttctcccgaa aaaaaacagg
    14161 gcttgcgccc tgtttctgta atacaaatga tgtattagta ctgatgagcg gtcgcgttgt
    14221 taccaaagcc aacctgagtc acgttgacgg aggagttaga tgcagtctgg tcaactgcag
    14281 caccgttgcc accaccgaac tgtttaaccg tcatttcaga atttttgccg ttccactgat
    14341 caagagtagc gctgttaccg aagccacgtt gggtcagatc gattgagctg tcatccgagc
    14401 cctgaccaac atctgcacca ttaccgccgc catgctgggt aatagtcaag tcagagttac
    14461 gggcatcagt ttgcagagca agtgcagagt taccgccacc gtactggtaa atgttcagct
    14521 cagaatttgg gccgctatta ttaccgccac caccgtggtt accgccgccg ccgtactgag
    14581 gaacaacacc tgccagagcg ctaccggaga atacgattgc tgcaattgct gctactttta
    14641 aaagtttcat gtaaaacccc catcggattg atttaaaagt cgaatggaaa ttaacgttgt
    14701 gtcacgcgaa tagccatttg cgactgtctc tgcactacaa ttgccgtttt ttgagtacca
    14761 tactgtgtaa tatttgcttt attaccagaa cctttctgga taatcatcgc agtattacca
    14821 taagcacctt gcgaaatact ggcatcgttg gcactgcccg cctgatcaat atatgcaagg
    14881 ttataatctc ctgtctggtc aatctttgcc cggttgctac taccttcttg cgcaacaacc
    14941 gccaaaagtt ttgagcctcc ctgccgtaac tgagcactat tattagtccc agcttgacca
    15001 attatggctg cctgattaaa tgaagactta ctcaattcat ttaccgcgaa gttatattct
    15061 gaattagcta aatcataacc tgctgcggct gcaatcccag gcgcacccag tattgttaac
    15121 atcataaata acaatttgtt tttcatgttg tcaccctgga cctggtcgta catttaagaa
    15181 attaaatcat ttcaacttgg ttgttaacgc aacctgtatt ttgttaacgc tgcgttacga
    15241 tggaaagtat gtctgcggaa atatttttaa taactcaccc gcgcgttgta tttttctttt
    15301 tttgttcata ccaaagtatt aaaacccacg taaatacgct gttatctacg caaaaaatat
    15361 tttgttttct tttaaatctc cgttttccgc tatcaaaaat caccagacag tcatttttct
    15421 tgcccgtcgc tgattgctgc gatatgtctt gcgcacaagc cgtgacaaac ctcgtctttt
    15481 tggagtaaaa atttgtccac ggaggtgtgg agaaaaaaca acaacttttt acatgacgaa
    15541 aggactacac
//
LOCUS       DAJSKJ010000086        15537 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000086 DAJSKJ010000000
VERSION     DAJSKJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.086"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..875
                     /locus_tag="ON345_002332"
     CDS             <1..875
                     /locus_tag="ON345_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3072385.1"
                     /translation="TLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGV
                     QLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILP
                     AVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDEL
                     VVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNS
                     EAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNAL
                     RRFLDYCDPANVPR"
     gene            1080..2549
                     /gene="lysP"
                     /locus_tag="ON345_002333"
     CDS             1080..2549
                     /gene="lysP"
                     /locus_tag="ON345_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR3072386.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2842..4833
                     /gene="cirA"
                     /locus_tag="ON345_002334"
     CDS             2842..4833
                     /gene="cirA"
                     /locus_tag="ON345_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR3072387.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLSLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFSDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4865..5701)
                     /gene="yeiG"
                     /locus_tag="ON345_002335"
     CDS             complement(4865..5701)
                     /gene="yeiG"
                     /locus_tag="ON345_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR3072388.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5959..6627
                     /gene="folE"
                     /locus_tag="ON345_002336"
     CDS             5959..6627
                     /gene="folE"
                     /locus_tag="ON345_002336"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR3072389.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6644..7801
                     /locus_tag="ON345_002337"
     CDS             6644..7801
                     /locus_tag="ON345_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR3072390.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7943..8983
                     /gene="galS"
                     /locus_tag="ON345_002338"
     CDS             7943..8983
                     /gene="galS"
                     /locus_tag="ON345_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR3072391.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            9263..10261
                     /gene="mglB"
                     /locus_tag="ON345_002339"
     CDS             9263..10261
                     /gene="mglB"
                     /locus_tag="ON345_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR3072392.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10322..11842
                     /gene="mglA"
                     /locus_tag="ON345_002340"
     CDS             10322..11842
                     /gene="mglA"
                     /locus_tag="ON345_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR3072393.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11858..12868
                     /gene="mglC"
                     /locus_tag="ON345_002341"
     CDS             11858..12868
                     /gene="mglC"
                     /locus_tag="ON345_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR3072394.1"
                     /translation="MSALNKKSYLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12939..14174)
                     /gene="preA"
                     /locus_tag="ON345_002342"
     CDS             complement(12939..14174)
                     /gene="preA"
                     /locus_tag="ON345_002342"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR3072395.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14168..15406)
                     /gene="preT"
                     /locus_tag="ON345_002343"
     CDS             complement(14168..15406)
                     /gene="preT"
                     /locus_tag="ON345_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR3072396.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSAQELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQCNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         3696 a         3815 c         4084 g         3942 t
ORIGIN      
        1 tcaccctccg gcagttggaa gtttttgcag aagtattgaa aagtggatca accacccagg
       61 cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc agccttgacc gacctggaag
      121 ggcagcttgg cgtgcaactg tttgatcgcg tggggaaaag actggttgtt aatgaacacg
      181 ggcggctgct ctatccgcgt gcgttggcat tgcttgaaca ggcggttgaa atcgaacaac
      241 tgtttcgcga agacaacggc gcgattcgta tctatgccag tagtaccatc ggtaactaca
      301 ttctgcctgc agttatcgcc cgttatcgcc atgattatcc gcagttgccg attgaactta
      361 gcgttgggaa cagccaggac gtgatgcaag cggtgctgga tttccgcgtt gatattggct
      421 ttattgaagg accgtgccac agcactgaaa tcatttctga accgtggctg gaagacgagc
      481 tggtggtttt cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc ttagaacagc
      541 tggccgctgc gccgtggatc ctgcgtgaac gcggttccgg cacgcgggag attgtcgatt
      601 atctattgct gtcacattta ccgaagtttg agatggcgat ggaattaggt aactccgagg
      661 caatcaaaca tgcggtgcgt catgggttgg gaattagttg cctgtcgcga cgggtgattg
      721 aagatcaatt gcaggcaggc acattaagtg aagttgcggt ccctctgccg cgcctgatgc
      781 gtacgttgtg gcgtatacat catcggcaaa aacacctttc caacgcgcta cggcgctttc
      841 tggactattg cgatcccgcg aatgtaccgc gttaagttgc tgtacaagaa catgctggtg
      901 ctgtgtcgat ttcgtgacgc agcgccctca gcatgcattc gccagaaaag agattggctg
      961 ctttacttat aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga
     1021 aggattgcca atcgtctgct acaatcgcgc ctcattttta agatggatag catttttgta
     1081 tggtttccga aactaaaacc acagaagcgc cgggcttacg ccgtgaatta aaggcgcgtc
     1141 acctgacgat gattgccatt ggcggttcca tcggtacagg tctttttgtt gcctctggcg
     1201 caacgatttc tcaggcaggt ccgggcgggg cattgctctc gtatatgctg attggcctga
     1261 tggtttactt cctgatgacc agtctcggtg aactggctgc atatatgccg gtttccggtt
     1321 cgtttgccac ttacggtcag aactatgttg aagaaggctt tggcttcgcg ctgggctgga
     1381 actactggta caactgggcg gtgactatcg ccgttgacct ggttgcagct cagctggtca
     1441 tgagctggtg gttcccggat acaccgggct ggatctggag tgcgttgttc ctcggcgtta
     1501 tcttcctgct gaactacatc tcagttcgtg gctttggtga agcggaatac tggttctcac
     1561 tgatcaaagt cacgacagtt attgtcttta tcatcgttgg cgtgctgatg attatcggta
     1621 tcttcaaagg cgcgcagcct gcgggctgga gcaactggac aatcggcgaa gcgccgtttg
     1681 ctggtggttt tgcggcgatg atcggcgtag ctatgattgt cggcttctct ttccagggaa
     1741 ccgagctgat cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac attccacgcg
     1801 cggtacgtca ggtgttctgg cgaatcctgt tgttctatgt gttcgcgatc ctgattatca
     1861 gcctgattat tccgtacacc gatccgagcc tgctgcgtaa cgatgttaaa gacatcagcg
     1921 ttagtccgtt caccctggtg ttccagcacg cgggtctgct ctctgcggcg gcggtgatga
     1981 acgcagttat tctgacggcg gtgctgtcag cgggtaactc cggtatgtat gcgtctactc
     2041 gtatgctgta caccctggcg tgtgacggta aagcgccgcg cattttcgct aaactgtcgc
     2101 gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt gattgccggt ctgtgcttcc
     2161 tgacctccat gtttggcaac cagacggtat acctgtggct gctgaacacc tccgggatga
     2221 cgggttttat cgcctggctg gggattgcca ttagccacta tcgcttccgt cgcggttacg
     2281 tattgcaggg acacgacatt aacgatctgc cgtaccgttc aggtttcttc ccactggggc
     2341 cgatcttcgc attcattctg tgtctgatta tcactttggg ccagaactac gaagcgttcc
     2401 tgaaagatac tattgactgg ggcggcgtag cggcaacgta tattggtatc ccgctgttcc
     2461 tgattatttg gttcggctac aagctgatta aaggaactca cttcgtacgc tacagcgaaa
     2521 tgaagttccc gcagaacgat aagaaataag tttcctccct tccttgctaa gccctctcaa
     2581 ccgagagggc tttttcaatt ccatttccct gacaaatcat gcggatataa aatttaacat
     2641 ttggattgat aattgttatc gtttgcatta tcgttacgcc gcaatcaaaa aaggctgaca
     2701 aatcagaggc tgttccggct ttctgggatg atcacctgca tacaaataag tccaccgcga
     2761 tgctgccgta cgcaagggga cgtgaagaag atgtgagcga taacccattt tattttcgta
     2821 gttacctcat ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt cgggctgtgt
     2881 ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg atgacgatgg cgaaacgatg
     2941 gttgtcactg catcttccgt tgaacaaaac cttaaagatg cacccgccag tatcagcgtc
     3001 attacccagg aagatctgca gcgaaaaccg gtacagaatc tgaaggatgt cctcaaagaa
     3061 gtgcctggcg tacaactgac gaacgaaggg gataaccgta agggcgttag tattcgtggt
     3121 ctggacagca gctataccct gattctcgtc gacggtaaac gcgtgaactc ccgcaatgcc
     3181 gtcttccgcc acaatgattt cgatctgaac tggatcccgg tcgattccat cgaacgtatt
     3241 gaagtggtcc gtggcccgat gtcgtcgctg tacggttccg atgcgctcgg cggtgtagtg
     3301 aatatcatca ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt cgataccacc
     3361 attcaggaac atcgcgatcg cggtgatacc tataacggtc agttctttac cagtggacca
     3421 ttaattgatg gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg tgaaaaggat
     3481 gacccgcaaa actcaacgac caccgatacc ggagaaacgc cgcgtattga aggattctcc
     3541 agccgcgacg gcaatgtcga atttgcctgg acaccgaatc aaaatcacga ttttactgcc
     3601 ggatacggtt tcgaccgtca ggatcgtgat tccgactcgc tggacaaaaa ccgcctggaa
     3661 cgccagaact actccgtcag ccataatggg cgttgggatt acggcaccag cgaactgaaa
     3721 tactacggtg agaaagtcga gaacaaaaac cctggcaaca gcagcccgat aacttccgaa
     3781 agcaatacgg tcgacggcaa atacacgttg tcgctgacgg cgattaatca gtttctcacg
     3841 gttggcggtg aatggcgtca cgacaaactt agcgatgcgg tgaacctgac cgggggaacc
     3901 agctccaaaa cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg gcggatcttc
     3961 gagccgctgg cgctgacgac cggtgtgcgt atggacgatc acgaaaccta cggtgaacac
     4021 tggagtccgc gtgcctacct ggtttataac gccaccgaca ccgtaacggt gaaagggggc
     4081 tgggcgacgg catttaaagc accttctctg ttgcaactta gccctgactg gacgagcaat
     4141 tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga aaccagcgaa
     4201 agttgggagc tggggcttta ctacatgggt gaagaaggct ggctggaagg ggttgaatcc
     4261 agcgttaccg ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg tacgtctgac
     4321 gtcaatgctg caccgggcta ccaaaacttt gtcggttttg agacgggcgc taacggacgg
     4381 cgcataccgg tttttagcta ctacaacgtt aacaaagcgc gtattcaggg cgtggaaacc
     4441 gaactgaaaa ttccgttcag cgatgaatgg aaactgtcga tcaactacac ctacaacgat
     4501 ggtcgtgatg tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt ccataccgct
     4561 aacggtacgc tggactggaa accgctggcg ctggaagact ggtcattcta tgtttctggt
     4621 cactataccg ggcagaaacg agccgacagc gcgacggcta aaacacctgg cggttatacc
     4681 atctggaata ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg cgcaggcgtg
     4741 ctgaaccttg gcgacaagga tcttagtcgt gacgactaca gctataacga agacggacgt
     4801 cgttacttta tggcagtgga ttatcgcttc tgatgagaag atgcccggcg aaccgggcgg
     4861 actttcactt cagtaaatac tgcgcatgga agcgcaggtg atcctctata aaagaggcga
     4921 tgaagtagta actgtgatca tatcccggct gaatacgcag cgtcatcggc cacgctttct
     4981 ggcgggcggc ttctgccaga actgcgggtt gcaactggtc ggcaagaaac tgatcattat
     5041 cgccctgatc gataagtgtc gggattgcat cctgcgcatt gctggcgtac atcaacgcac
     5101 aactgtccca ttccagccat gcatttttgt cctcacctaa ataggtgcta aacgctttaa
     5161 tgccccaggg tacgctgcac ggattcacaa ttggcgcaaa ggccgaaacg ctagtgtatt
     5221 tacccggatt tttcagcgcc ataatcagcg caccgtgacc acccattgaa tgaccgctaa
     5281 tggcgcagcg gtcgctgaca ttaaattgcg actgaaccag cgccggtaat tcatcgcgca
     5341 gataatcata catccggtaa tgcgtcgccc acggcggttg cgtggcgtta agataaaacc
     5401 ccgcgccctg gcctaaatcg tagccatcat cgttggcaac ctgttcgccg cgcgggctgg
     5461 tgtctggcat caccagtaca atccccagtt ctgccgccac gcgctgggca cccgccttga
     5521 tggtgaagtt ctcgtcattg caggttaatc cggaaagcca gtacagcact ggtggcgggg
     5581 tgtgatcacg aggtggtggg agaaagatac tgaacgtcat cggacagttt aaggtactgg
     5641 agtcgtgtcg ccagcgttgc tgccagcctt caaaacagcg gtgctcttcg agcatttcca
     5701 tgcgtggctc cttgttgtgt tgtttgcaat ttcttcatca taccgtttat tcaatgtgct
     5761 gtgagtaact ttcacttccg tatttgcata acgatgtttt aacatctgct gatgaaaggc
     5821 agcggcaatt acaataatta tcgctgtgaa tactggatta tgtgcgccgc ctcacgcaca
     5881 ataatcaagc tgtaaatcag cttaataact ttgcccccac gcagggcgga ggcgtcacac
     5941 ctgcaggaga aatcataaat gccatcactc agtaaagaag cggccctggt tcatgaagcg
     6001 ttagttgcgc gaggactgga aacaccgctg cgcccgcccg tgcatgaaat ggataacgaa
     6061 acgcgcaaaa gccttattgc tggtcatatg accgaaatca tgcagctgct gaatctcgac
     6121 ctggctgatg acagtttgat ggaaacgccg catcgcatcg ctaaaatgta tgtcgatgaa
     6181 attttctccg gtctggatta cgccaacttc ccgaaaatca ccctcattga aaacaaaatg
     6241 aaggtcgatg aaatggtcac cgtgcgcgat atcactctga ccagtacctg tgaacaccat
     6301 tttgttacca tcgatggcaa agcgacggtg gcttatatcc cgaaagattc ggtgatcggt
     6361 ctgtcaaaaa ttaaccgtat cgtgcagttc tttgcccagc gtccgcaggt gcaggaacgt
     6421 ctgacgcagc aaattcttat tgcgctacaa acgctgctgg gcaccaataa cgtggctgtc
     6481 tcgatcgacg cggtgcatta ctgcgtgaag gcgcgtggca tccgcgatgc aaccagtgcc
     6541 acaacaacga cctctcttgg tggattgttc aaatccagtc agaatacgcg ccacgagttt
     6601 ctgcgcgctg tgcgtcatca caactgatta aaaggcagga accatggagc gcaacgtcac
     6661 gctcgatttt gttcgcggcg tcgccattct ggggatcctg ctattaaaca tcagcgcctt
     6721 tgggctacca aaggcggctt atctcaatcc cgcctggtac ggcgctatta cgccgcagga
     6781 tgcatggacc tgggcatttc tcgatctcat cggccaggtg aaattcctca cgctttttgc
     6841 gctgctgttt ggtgcgggcc tgcaaatgtt gctgccccgt ggtagacgct ggatccagtc
     6901 gcggttaacg ctgttagtct tgctgggttt tattcacggt ttattgttct gggacggcga
     6961 tattctgctg gcttacgggc tggtgggctt aatctgctgg cggctggtgc gcgatgcgcc
     7021 atcggtaaaa agcctgttta ataccggcgt catgctttat ctggtggggc ttggcgtttt
     7081 gctgttattg gggttgattt ccgatagcca gaccagccgc gcctggacgc cggatgcatc
     7141 ggctattttg tatgaaaaat actggaagct tcacggcggc gttgatgcga tcagtaatcg
     7201 tgccgatggt gttggcaaca gtttactggc actgggcgca cagtatggct ggcaactggc
     7261 tgggatgatg ctcattggtg cggcgctgat gcgcagcggc tggctgaaag ggcagttcag
     7321 cttacgtcac tatcgtcgta ctggttttgt gctggtggcg attggggtga ccattaacct
     7381 tcctgccatc gccctgcaat ggcagctgga ctgggcgtat cgctggtgcg ccttcttact
     7441 tcagatgccg cgggaactga gtgcgccgtt tcaggcgatt ggctatgcgt cgctgtttta
     7501 tggcttctgg ccgcaattga gccgctttaa gctggtgctt gcgatcgcct gcgtcggacg
     7561 gatggcgctg accaactatc tattgcaaac gctgatttgt accacgcttt tttaccacct
     7621 cggtttgttt atgcattttg accgtctgga gctgctggcg tttgttattc cggtatggct
     7681 ggcgaatatc ctcttctctg ttatctggct gcgttacttc cgccaggggc cggtggaatg
     7741 gctctggcgt cagttaactt tgcgtgctgc cggaccggca atatctaaaa catcaagata
     7801 acgatctgga tcacattcgt taacaaaacg gctgtaaccg tttccattgc tgtgactcga
     7861 ttcacgaagt cctgtattca gtgctgacaa aatagccgcc agcaagcagt catttactgc
     7921 aatctcataa caggtagtga atatgatcac cattcgtgat gtagcgcgtc aggctggcgt
     7981 ctctgtggca acggtttccc gggtgctcaa taacagcacg ctggtcagtg ccgacacgcg
     8041 tgaagcagta atgaaagccg tgagtgagct ggattatcga ccaaatgcca atgcccaggc
     8101 gctggcaact caggttagcg ataccattgg cgtggtggtg atggacgttt ctgatgcttt
     8161 tttcggcgcg ctggtaaaag cggtggatct ggtcgcccag cagcatcaga aatacgtgct
     8221 aatcggcaat agctatcatg aagcggaaaa agagcgtcac gccattgagg tgttaattcg
     8281 ccagcgttgt aatgcgttga ttgttcactc aaaagcattg agtgacgatg aactggcgca
     8341 atttatggat aacattcccg gtatggtgtt aatcaaccgc gttgtgccgg ggtacgccca
     8401 tcgttgcgtt tgcctggata atctcagcgg tgcccgaatg gcgacgcgca tgttgctgaa
     8461 taacggtcat caacgtattg gttatctttc ttccagccac ggcattgaag atgacgccat
     8521 gcgtaaagca ggctggatga gtgcgttgaa agagcaggat attattccgc cggaaagctg
     8581 gattggcact ggtacgccgg acatgccggg cggtgaggcg gcgatggttg aactgctggg
     8641 gcgcaatcta caacttaccg ctgtatttgc ttataacgac aatatggctg ctggcgcact
     8701 gacagcatta aaagataatg gcattgcgat tccgttacat ctctcaatca tcggtttcga
     8761 tgatattccc atcgcccgtt acaccgaccc gcaattaacg accgtgcgtt atcccattgc
     8821 ttcaatggcg aaattagcca ccgaactggc cttgcagggg gcagcaggca atattgatcc
     8881 tcgtgccagc cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg caacacgcca
     8941 gaatgcggcg acgatcacta actcaacaaa tcaggcgatg taaccgcttt caatctgtga
     9001 gtgatttcac agtatcttaa caatgtgata gctatgattg caccgtttta acgttgtaac
     9061 ccgtatgtaa cagtgaataa tcactttcac cgaggtaata gcgtcataac aacaattaaa
     9121 gccgttttct ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg agcttcggcg
     9181 ttcagtaaca cttcattaac tctactgccc cgccgagcat ttatctcaag cactaccctg
     9241 cataagaaaa accggagata ccatgaataa gaaggtgtta accctgtctg ctgtgatggc
     9301 cagcatgtta ttcggtgccg ctgcacacgc tgctgatact cgcattggtg taacaatcta
     9361 taagtacgac gataacttta tgtctgtagt gcgcaaggct attgagcaag atgcgaaagc
     9421 cgcgccagat gttcagctgc tgatgaatga ttctcagaat gaccagtcca agcagaacga
     9481 tcagatcgac gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc tggttgaccc
     9541 ggcagctgcg ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg tggttttctt
     9601 caacaaagaa ccgtctcgta aggcgctgga tagctacgac aaagcctact acgttggcac
     9661 tgattccaaa gagtccggca ttattcaggg cgatttgatt gctaaacact gggcggcgaa
     9721 tcagggttgg gatctgaaca aagacggtca gattcaattc gtattgctga aaggtgaacc
     9781 gggccatccg gatgcagaag cacgtaccac ttacgtgatt aaagagctga acgacaaagg
     9841 cattaaaact gaacagttac agttagatac cgctatgtgg gataccgctc aggcgaaaga
     9901 taagatggac gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg ttatcgccaa
     9961 caacgatgcg atggcaatgg gcgcggtaga agcactgaaa gcacacaaca agtccagcat
    10021 tccggtgttt ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat ccggtgcact
    10081 ggcgggcaca gtactgaacg atgctaacaa ccaggcgaaa gcgacctttg atctggcgaa
    10141 aaacctggcc gatggcaaag gtgcggctga tggcaccaac tggaaaatcg acaacaaagt
    10201 ggtccgcgta ccttatgttg gcgtagataa agacaacctg gctgaattca gcaagaaata
    10261 agactgattg ggcgcagtcg tttactgcgc ccttattgta tggccaacaa ggtaaaatta
    10321 tatggtcagc tcaacgactc cgtcttccgg ggaatacttg ttggaaatga gcggtatcaa
    10381 caagtctttt cctggtgtta aggcacttga taacgttaat ttaaaagtcc ggccacattc
    10441 tatccatgca ttaatggggg aaaacggcgc aggaaaatca acattattaa aatgcctgtt
    10501 tggtatttat caaaaagact ccggcaccat tttattccag ggtaaagaga tcgatttcca
    10561 ttctgccaaa gaagcactgg aaaatggtat ttcgatggta caccaggagt taaacctggt
    10621 attacaacgt tcggtgatgg ataacatgtg gctggggcga tatcccacca aaggcatgtt
    10681 tgtcgatcag gacaaaatgt accgcgaaac caaagcgatt tttgatgaac tggatattga
    10741 tatcgatccg cgtgcgcgcg tcggcacatt atccgtttcg caaatgcaga tgatcgaaat
    10801 cgccaaagcg ttttcctata acgcgaaaat tgtgattatg gatgaaccga cttcttcgtt
    10861 aaccgaaaaa gaggtcaatc atctgttcac tattattcgt aaattaaaag agcgcggctg
    10921 cggtattgtt tatatctcgc ataaaatgga agaaatcttc cagttatgtg atgaagttac
    10981 cgtattgcgc gacggtcagt ggatcgccac cgaaccgctg gcaggactga cgatggacaa
    11041 gatcatcgcc atgatggttg ggcgttctct taatcagcgt tttcctgaca aagaaaacaa
    11101 gccgggcgaa gtcatcctcg aggtgcgtaa cctaacgtca ctgcgccagc cgtcgattcg
    11161 cgatgtctcg tttgatctgc ataaagggga gatcctcggt attgccgggc tggtgggggc
    11221 gaaacgtacc gatattgttg agacgttatt tggtattcgc gagaaatcgg ctggcaccat
    11281 tacgttgcac ggcaaacaga tcaataacca taatgccaac gaagccataa accacggatt
    11341 tgcactggta actgaggagc gccgctcaac gggaatttat gcctatctgg atattggttt
    11401 taactcgtta atttccaata ttcgcaacta caaaaataaa gttggtttac tggataactc
    11461 gcggatgaaa agcgataccc agtgggtgat tgattcgatg cgggtaaaaa cgccgggtca
    11521 tcggacgcaa attggttcgc tctccggtgg taatcagcaa aaggtgatta ttggtcgctg
    11581 gctactaacg caaccagaaa tattaatgct cgatgaaccg acgcgcggta ttgatgtcgg
    11641 ggcgaagttt gaaatttatc agttaattgc cgaactggcg aagaaaggca aggggattat
    11701 tattatctcc tctgaaatgc ctgagttgtt agggataaca gaccgtattc tggtcatgag
    11761 caatggtctc gtttccggaa ttgtcgatac aaaaacaaca acgcaaaacg aaattctgcg
    11821 tcttgcgtct ttgcaccttt aagattaggg gcttcccatg agtgcgttaa ataagaaaag
    11881 ttatcttact tacctgaaag agggcggtat ttacgtcgtt cttttagttt tgctggcgat
    11941 tattattttc caggacccaa catttttaag tctgttgaac ttaagtaata ttctcaccca
    12001 gtcatcggtg cgtattatta tcgcgctcgg tgtggcaggg ttaattgtca cccaggggac
    12061 cgatctttct gctggtcgtc aggtagggct ggcggcagtg gtggctgcga cattattgca
    12121 gtccatggat aacgccaaca aagtgttccc ggaaatggcg acgatgccga ttgcgctggt
    12181 tattctgatt gtctgtgcca ttggtgcggt gatcggtttg atcaacggtc tgattatcgc
    12241 ttatctcaac gtgacgccgt tcattaccac gctcggcacg atgatcatcg tctatggcat
    12301 caactcgctc tattacgact ttgtcggggc gtcgccaatt tctggttttg acagtggctt
    12361 ctctaccttt gctcagggct ttgtcgcgct ggggagtttc cgtctctctt acatcacctt
    12421 ctacgcgttg attgcggtgg cgttcgtctg ggtgttgtgg aacaaaaccc gcttcggtaa
    12481 gaacattttt gccattggcg gtaacccgga agcggcaaaa gtatctggtg tcaacgtcgg
    12541 cctgaacctg ctgatgatct acgcgttgtc tggcgtgttc tatgcctttg gcgggatgtt
    12601 agaagccgga cgtatcggct ctgccaccaa caacctcggc tttatgtatg agctggatgc
    12661 tatcgcggcg tgcgtggtag gcggtgtatc gttcagcggc ggtgtgggga cggtgattgg
    12721 cgtggtgacc ggggtaatta tttttaccgt catcaactat ggcctgacgt atatcggcgt
    12781 aaacccatac tggcagtaca tcatcaaagg ggcgattatt atcttcgccg tagcgctgga
    12841 ttcactgaaa tacgcgcgta agaagtgatg tgattgtgga tattgccgga tgcgctgcat
    12901 ccggcaatcg gtgccaaatg ccggatgcgt ctctggtttt acaacgttac cgggtgttct
    12961 ttctcgcctt tcttaaactt cacttccccg agctcaatac aacccaccgg gcagacgtga
    13021 ccacaaagca gacaacccac acatttctcg gtattacaat gcggtgtgcg ggttttctcg
    13081 ctccattcca tcgcctggtg accgccgtcg taacaggaaa tataacagcg tccacagcca
    13141 acacatttat caagattgat acggggatag acaatataac tgcggtctaa atcttccgcc
    13201 gggacaatat tgttattcgc cagacctacc atctcctgca gcgaatcaaa tccctgatcg
    13261 gcgagataat gcgacaaccc gcttgccata tcttccacta tccgataccc gtactgcatg
    13321 atgccggtgg tcacctgtaa cgttgctgcg ccgagcaata aaaactcagc cgcatcctcc
    13381 caggtttcaa tgccgccgat accgctgatt gggaaatcgc gcagttctgg atgggtgcgc
    13441 atttgctgaa tgaagcgcag ggcgatcggt tttaccgctt taccggaata tccggaaata
    13501 ctcgattttc cgttaacgat cggcataccg attttctgat taagatcgat attggtgatg
    13561 gatttaacgg tgttaatcgc cgcaatgcca tctgcgccgc cgcgcttcgc cgccagcgcc
    13621 acttcgcaca tatcaccgat attcggcgtc atcttcgcca gcattggcag cgtggaaccc
    13681 cgtttcactg cccgacaata tttttctacc agctccgggc tttgcccgac atcgctaccc
    13741 atcgcatgag aagtcatttg cggacaggag aagttacact cgatcatatc cgcgccagct
    13801 tcttgcacca ggcgcgccag ctcctcccat tgctgctcat tttcccccat gatcgaagcg
    13861 atcaatactt tgtccgggta atcttccttc agccgacgca gggcggccag attctcttcc
    13921 aacggatgtt cagcaatctg ctccatattt ttgaagccga taaaaccggt atcttctttc
    13981 accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat cgttttaaac
    14041 accacaccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca gttgcctacc
    14101 ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaagt aatcgaaaga
    14161 tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg gcggcttctt
    14221 tcccggtttt cacggcatag accacggttt tgtcaccctc aacaatatcg cccgcagcaa
    14281 agacttgcgg atcgcgggtc tgataatttt gtgtattgat agtgttacac tgcggctcta
    14341 actccgcaaa ggcatccagt ctggcatgct gaccgacggc gagaataatt ttatctgccg
    14401 ccatcgtcag ttcgcccggt aaccgtacat gcttaaacgt gactttattg ccttccacgg
    14461 ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ttgggcgctg gtaaactctt
    14521 tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttacc gcctgacagc
    14581 cgagaacttt cagcgtgctg gctacgtcca tcgcgacatc accgccgccg ataattaatg
    14641 cgctttgtgg aatgctgata tcgccttgcg cctgtcgtac acgttgcagg aagtcgacgg
    14701 caatctcaac gtcactatgc tcaaacagcg gtagcccgga accgcttgat aaccccacgg
    14761 tgaccagtac cgcgcggttt tctgctttca gctgctcaag ggtgagtgtg ttaccgactt
    14821 cgttgttgca cttaatggtc acgcccattt tttcaatacg ggcgatctct gcatccagca
    14881 ctgattgcgg taaccggaat tgcggaatac cgttacgcaa ccagccaccg gggtgcgctt
    14941 ctttctcata aatcgtgacg tcataaccct ggtttgtcag tgttacactg gcctgcaatc
    15001 cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg ggctgataaa
    15061 tttccatccc agtttgttgt tcaaaatcag taacaaaacg ctgtaagcga ccgatatcaa
    15121 tgggagtatc aacaccggca cgggtacaac cgctttgaca taatttctcc gtcgggcaca
    15181 ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca gcgcctttaa
    15241 aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc gggcaagcct
    15301 gactacaggg agcgtcatga cataataaac agcgagaggc ttctttaatc gccagtaaag
    15361 gcgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga tcttccttaa
    15421 tgctatgagt aattaaggcg agcgtttagt aaataataat gtactgatat taaggctaag
    15481 cgtgtgggga aatttaaact gtgaaaggat tcacaaatag ctgcggttca gaatgtt
//
LOCUS       DAJSKJ010000087        15477 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000087 DAJSKJ010000000
VERSION     DAJSKJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.087"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            91..609
                     /locus_tag="ON345_002344"
     CDS             91..609
                     /locus_tag="ON345_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR3072397.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            599..1825
                     /gene="dgcN"
                     /locus_tag="ON345_002345"
     CDS             599..1825
                     /gene="dgcN"
                     /locus_tag="ON345_002345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR3072398.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLTLTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1841..2323
                     /locus_tag="ON345_002346"
     CDS             1841..2323
                     /locus_tag="ON345_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR3072399.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2400..2747)
                     /gene="rplS"
                     /locus_tag="ON345_002347"
     CDS             complement(2400..2747)
                     /gene="rplS"
                     /locus_tag="ON345_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR3072400.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2789..3556)
                     /gene="trmD"
                     /locus_tag="ON345_002348"
     CDS             complement(2789..3556)
                     /gene="trmD"
                     /locus_tag="ON345_002348"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR3072401.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3587..4135)
                     /gene="rimM"
                     /locus_tag="ON345_002349"
     CDS             complement(3587..4135)
                     /gene="rimM"
                     /locus_tag="ON345_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR3072402.1"
                     /translation="MSKQLTAQAPIDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4154..4402)
                     /gene="rpsP"
                     /locus_tag="ON345_002350"
     CDS             complement(4154..4402)
                     /gene="rpsP"
                     /locus_tag="ON345_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR3072403.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4539..5900)
                     /gene="ffh"
                     /locus_tag="ON345_002351"
     CDS             complement(4539..5900)
                     /gene="ffh"
                     /locus_tag="ON345_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR3072404.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6067..6858
                     /locus_tag="ON345_002352"
     CDS             6067..6858
                     /locus_tag="ON345_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR3072405.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6879..8165
                     /locus_tag="ON345_002353"
     CDS             6879..8165
                     /locus_tag="ON345_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3072406.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GDRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8220..8813)
                     /gene="grpE"
                     /locus_tag="ON345_002354"
     CDS             complement(8220..8813)
                     /gene="grpE"
                     /locus_tag="ON345_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3072407.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8936..9814
                     /gene="nadK"
                     /locus_tag="ON345_002355"
     CDS             8936..9814
                     /gene="nadK"
                     /locus_tag="ON345_002355"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR3072408.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9900..11561
                     /gene="recN"
                     /locus_tag="ON345_002356"
     CDS             9900..11561
                     /gene="recN"
                     /locus_tag="ON345_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR3072409.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLVEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11710..12051
                     /gene="bamE"
                     /locus_tag="ON345_002357"
     CDS             11710..12051
                     /gene="bamE"
                     /locus_tag="ON345_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR3072410.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12113..12403)
                     /locus_tag="ON345_002358"
     CDS             complement(12113..12403)
                     /locus_tag="ON345_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR3072411.1"
                     /translation="MPGKIAVEVAYALPDKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12393..12830)
                     /gene="ratA"
                     /locus_tag="ON345_002359"
     CDS             complement(12393..12830)
                     /gene="ratA"
                     /locus_tag="ON345_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR3072412.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13001..13483
                     /gene="smpB"
                     /locus_tag="ON345_002360"
     CDS             13001..13483
                     /gene="smpB"
                     /locus_tag="ON345_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR3072413.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13698..14060
                     /gene="ssrA"
                     /locus_tag="ON345_002361"
     tmRNA           13698..14060
                     /gene="ssrA"
                     /locus_tag="ON345_002361"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14264..15477
                     /locus_tag="ON345_002362"
                     /pseudo
     CDS             14264..15477
                     /locus_tag="ON345_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417111.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         3926 a         4061 c         3674 g         3816 t
ORIGIN      
        1 ttgatgacga tcgcatgaaa aaatgatatt ttaatgagca ttctgaaaaa atatcgatgc
       61 atttcgagcg aagatggtgg ggatccctga atgcgttttt ctcaccgact gttcttactt
      121 ctgattctcc tgctgacggg agcaccaata ctcgctcagg agccttctga cgttgcgaaa
      181 aacgtgcgta tgatggtttc cggtattgtc agttacaccc gctggcctgc gctatcaggg
      241 ccaccaaagt tatgtatctt ttcatcttca cgttttagta ccgccctaca agagaatgcc
      301 gcgacatctt tgccttatct gcctgtcatc attcacactc aacaagaggc gatgatttca
      361 ggctgcaatg gtttttattt tggcaacgag tcaccgacat tccagatgga attaaccgaa
      421 caatatccgt caaaggcgtt attattaatt gccgaacaaa ataccgagtg cattattggt
      481 agcgcctttt gtctgatcat ccacaataat gacgtcagat ttgccgtaaa cctggatgcc
      541 ttatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa tgctcgcacg gaagaaaaat
      601 gatggataac gataattctc ttaataagcg ccccacgttt aaaagagcat tacgcaacat
      661 cagtatgacc agcatattta tcactatgat gctgatctgg ttgctgcttt ccgtgacctc
      721 ggtgctgacc ctgaaacagt acgcgcaaaa aaacctgaca ctgacagcag caacaatgac
      781 ttacagtctg gaagcagctg tcgtttttgc cgatggccct gcagcaactg aaacactggc
      841 agcgctgggc cagcaagggc aattttcaac tgcagaagta cgtgataagc agcaaaatat
      901 tctggcgtcc tggcattaca cccgtaagga tccaggcgat actttcagca atttcataag
      961 ccactggctc ttccccgccc ccatcattca gccgattcgt cacaatggtg aaaccattgg
     1021 cgaagtacgc ttaaccgctc gcgacagttc aatcagccat tttatctggt tttcgctcgc
     1081 cgtactgacc ggttgtattc tgctggcatc aggcatcgca attaccctca cccgccattt
     1141 gcacaatggc ctggtggaag cactgaaaaa tatcaccgat gtcgtacatg atgtgcgttc
     1201 caaccgcaat ttttcccgac gagtttcgga agaacgtatc gctgagtttc accgcttcgc
     1261 tctcgacttc aacagtctgc tggatgaaat ggaagagtgg cagcttcgtt tacaggctaa
     1321 aaatgcgcag cttctacgta ccgcgctaca tgacccatta accgggctgg ctaaccgcgc
     1381 agcgtttcgt agcggcatca acacgttgat gaacaattcc gatgcccgaa aaacgtcggc
     1441 gttactattt cttgatggcg ataatttcaa atacatcaat gatacctggg gtcatgcgac
     1501 gggcgataga gtcttgattg aaatcgcaaa acggttagct gaagttggcg ggctgcgaca
     1561 taaagcatac cgcctgggcg gcgatgaatt cgctatggtg ctctatgatg tacagtcaga
     1621 atctgaagtg cagcagatat gctcagcact gacacaaatc tttaatctcc cgtttgatct
     1681 tcataatggt catcagacca ccatgacatt aagcattggt tacgcgatga ccattgagca
     1741 cgcctctgcg gaaaaattac aagagcttgc cgatcacaat atgtatcagg ccaaacacca
     1801 gcgtgccgaa aagctggtga gataacaagg atatatcgat atgataaagc acctggtagc
     1861 ccccctgatt ttcacctcac taatactgac tggctgccag tcccctcagg gaaagtttac
     1921 tcctgagcaa gtcgccgcta tgcaatctta tggatttact gaatccgccg gcgactggtc
     1981 gctgggctta tcagatgcca ttctgttcgc aaaaaatgac tacaaattgc tcccggaaag
     2041 ccagcaacag atccaaacca tggcagccaa attggcctcg acagggctaa cacatgcccg
     2101 tatggatgga cacaccgata actatggtga agacagttac aacgaaggct tatcattgaa
     2161 acgggcgaat gtcgtggccg atgcatgggc tatgggtgga caaattccac gcagcaatct
     2221 caccacacag ggtttaggaa aaaaatatcc catagccagt aacaagaccg cccagggccg
     2281 cgccgagaac cgccgcgtcg cagtggtgat tactacccct taaaacacca gcaaacagat
     2341 aaaaaaaggg ccagccaatt ggccagccct tcttaacagg atgtcgctta agcgaaatct
     2401 tagttaagac gctctttgat acgagcagcc ttaccagtac gctcacgcag gtagtacagt
     2461 ttagctttac gaacagcacc acgacgtttg acagaaatgc tgtcaactac cggagagtga
     2521 gtctggaaga cacgctcaac gccttcgccg ttggaaattt tacgaacagt gaatgcagag
     2581 tgcagaccgc ggttacgaat agcgataacc acgccctcga atgcctgcag acgttttttg
     2641 gaaccttcaa caacccatac tttcacttcc acggtatcac ccggacggaa ggaaggtacg
     2701 tcctgcttca tctgctcttg ttcaagttgc ttaataatgt tgctcataat ttaatctctt
     2761 atcctgggta aactgatatc tcgggggctt acgccatccc atcatgttta tgttgctgtt
     2821 gtgcgtgttc cgttttgaac tccgccagca accttgcttg ctcttcagtc agagccaggt
     2881 tttccagaag ttcaggtctt ctaagccagg tacggcccag cgactgtttc aaacgccagc
     2941 gacgtatctc ggcatggttg cccgacagta acactggcgg aacttccatc ccttctaaca
     3001 cctcaggccg cgtatagtgc gggcaatcca gcaatccttc agcaaaggaa tcttccgttg
     3061 ccgaggcttc atgtcccagt actcccggaa taaaccggga aacggagtca atcagcgtca
     3121 ttgctggtaa ctcaccacca ctgagaacgt aatcgccgat tgaccattct tcgtcaattt
     3181 cggtttggat cacgcgctca tctatacctt cgtagcgacc gcacaccaga atcaattttt
     3241 gattcgttgc cagttcgctg acgcccgctt gatcaagctt gcgtccctgt ggtgacagat
     3301 aaatcacctt tgcgccttca cccgccgcgg cttttgctgc atgaatggcg tcccgcaagg
     3361 gttgcaccat cattaacatc cccggtccgc cgccgtaagg acgatcgtcc acggtacggt
     3421 gccggtcatg cgtgaagtcg cgaggactcc aactctggat gctcagcagg ccatttttaa
     3481 ctgcccggcc agttaccccg taatcggtaa ttgcgcggaa catttcagga aacaggctaa
     3541 ttatgccaat ccacatagcg ccgtctttta ccgtttatcc ggtggtttaa aaaccaggat
     3601 cccaatctac ttcgattgaa cgagtagtga gatcgacttt cttgataacc tgcccatcga
     3661 ggaacggtac gagacgttcc ttgataccaa acgcatcttt caggtttgcc ttaatgacga
     3721 gaacgtcatt agatccggtt tccatcatat cgacgacttt accgagatcg tagccttcag
     3781 tggttactac ctggcagccc atcaggtctt tccagtagta gtccccctct tcaagctgag
     3841 gcagctgcga tgaatccacg acaatttcac aattcgtcag caggttcgcc gcatcacgat
     3901 cgtcaacgcc tttcagcttg atgatcatgt cctgattgtg gtgcttccag ctttccagct
     3961 ggacttgctg ccactgaccc gccttctgga taaaccaagg ctgatagtca aaaatgcttt
     4021 cggcgtcttc ggtggaagaa aacactctga gccacccacg aataccgtaa gacgaaccca
     4081 tttttcccaa aacgatggga tcaataggtg cttgcgcggt gagttgtttg ctcatcatga
     4141 ccaccgtgac agattaagct gctttgttta cttctttgat cagcgcagca acgcgatcag
     4201 aaatagttgc gccctggcca acccagtgag cgatgcgatc cagatccagg cgagtgcctt
     4261 cttctttttc gctagcgatt gggttgaaga aaccaacgcg ctcgatgaag cgaccgttgc
     4321 gtgcattacg gctgtcagcg acaacaacct ggtagaacgg acgcttttta gcgccgtgac
     4381 gtgctaaacg aatagttacc ataacatcct cttgtgtgaa taaaacaacc ggaccccatc
     4441 gaggaacgga gtccggtgtc atattaaaag cccgaaaatt ttactgattt ctggagaaaa
     4501 tgcaatcagc agttaataac tctatcgcat aaggtcattt agcgaccagg aaatcccggt
     4561 ggcatcatcc ctttcatgct tctcatcatc ttcgccattc cgcctttctt cattttcttc
     4621 atcatgcgct gcatgtcgtc gaactgcttc agaaggcggt tcacgtcctg cacctgcata
     4681 ccgcaaccgg cagcgatacg acgcttacgc gaacctttga tgatttctgg cttagcgcgc
     4741 tctttcatcg tcatcgagtt gatgatggct tccatacgca ccagcacttt atcgtccatc
     4801 tgcgacttaa cattatccgg gatctgcccc atgcccggca gcttgcccat caggctagcc
     4861 atgccgccca tatttttcat ctggcgcagt tgctcaagaa aatcgttgag atcgaagccg
     4921 tcgccttttt tcagcttgct ggctaatttc tctgcctgcg cgcggtcaac tttgctttcg
     4981 atatcttcga tcagcgacag tacgtcgccc atgccgagaa tacgcgacgc gatgcggtcc
     5041 ggatggaacg gctccagcgc ctcagttttc tcgccaacac cgagaaactt gatcggtttg
     5101 ccagtgatgt gacgaataga gagcgccgca ccgccgcggg catcgccgtc cactttggtc
     5161 aacactacgc cggtaagcgg taacgcttca ttgaatgctt ttgccgtatt ggccgcatcc
     5221 tgaccggtca tggcgtcaac cacaaacagg gtttcaaccg ggttaatcga cgcatggact
     5281 tgtttgatct cgtccatcat cgcttcgtca acgtgcagac gaccagcggt atccaccagc
     5341 agcacgtcgt agaatttcag tttggcttct ttcagcgccg cgttaacgat atctaccggc
     5401 ttctgaccaa catcagaagg gaagaaatca acgcccacct gctctgccag cgtctcaagc
     5461 tgtttgattg ccgccgggcg ataaacgtcg gcagaaacca ccagcacttt cttcttgtgc
     5521 ttctcgcgca ggaacttacc gagtttacca acgctggttg ttttaccggc accttgcagg
     5581 cccgccatca gcacgaccgc aggcggttgc gccgccaggt tcagggtctg gttctcttcg
     5641 cccatcgccg caaccagttc gttacggacg attttgacga actcctgccc cggcgtcagg
     5701 ctcttattaa cttcatgacc aaccgctttc tcttttacgc gattgataaa ctcacgcact
     5761 accggcagag cgacatccgc ctccagcagc gccatgcgca cttcgcgcag cgtatctttt
     5821 acgttgtctt cagtgaggcg tccacggcca ctgatattgc gcagcgtgcg cgacaaacga
     5881 tcggttaaat tatcaaacat tgtctctcgc ctggggtgga aacggttggc cgcaatcgcg
     5941 acacatcatc agtattttgc cgcagtataa catgaaggcg tctttgttgt tatgcaacgg
     6001 ttggagcagc gttcacctga cgctatactg cttctctttc ttattgctca aactgtcgac
     6061 atcactatgc ccgtttttgc tctgctcgcg cttgtcgcct actccgtcag tcttgcgctg
     6121 attgttcccg gtctgctgca aaaaaacggc ggctggcggc gcatggctat tatttctgcg
     6181 gtcattgcgc tggtctgcca cgcaatcgct ctggaagccc gcatcctgcc cgacggtgat
     6241 agcggacaaa acctcagcct gctgaacgtt ggttcattgg tcagtttgat gatctgtacg
     6301 gtaatgacca ttgtggcttc tcgcaatcgt ggctggttgc tgctacccat tgtctatgcc
     6361 tttgcgctta tcaacctggc gctggcgacc ttcatgccca atgaatacat cacccatctg
     6421 gaagcaacgc ctgggatgct ggtgcacatt ggcttatcgc tcttttccta tgccacgcta
     6481 attatcgccg ccctgtacgc gctgcaactg gcgtggattg attaccaact gaagaacaag
     6541 aagctggcat ttaaccagga aatgccgcca ttgatgagta tcgagcgtaa aatgttccac
     6601 atcacgcaga ttggcgtggt gctgctaacg ctcacgcttt gcactggcct gttctacatg
     6661 cacaacctat ttagcatgga aaatatcgac aaggctgtgc tctctatcgt ggcgtggttt
     6721 gtctatattg tgctgctgtg gggacattac catgaaggat ggcgtggacg ccgcgtcgtc
     6781 tggtttaacg ttgcgggcgc ggtaattctg acactggcct acttcggcag ccgaattgtc
     6841 cagcagttaa tcagctaaac ccagaaaagg agtttcccct ggaacacatt tctactacta
     6901 cgttgatcat tattctgatc atcatggtgg tcatttcagc ctatttttcc gggtccgaaa
     6961 ccggaatgat gaccctcaac cgctatcgtc tgcgacatat ggcgaaacag ggtgatcgct
     7021 cggccaaacg cgtcgaaaaa ttgctgcgta agccagaccg cctgataagc ctggtgttaa
     7081 tcggcaataa cctggtcaat attcttgcct ccgcgctcgg cactattgtt gggatgcgtt
     7141 tgtacggcga tgcgggcgtg gcaattgcga ctggagtgct gacttttgtc gtactggtat
     7201 ttgctgaggt attgccgaaa accattgccg cgctgtaccc ggaaaaagtc gcttatccga
     7261 gtagttttct gctggctccg ctgcaaattt tgatgatgcc gctggtctgg ttgctgaatg
     7321 ctatcacccg tatgctgatg cgcatgatgg gtatcaaaac cgatatcgtg gttagcggct
     7381 ctttgagcaa agaagagttg cgcactatcg tgcacgaatc acgctcacaa atttcccgtc
     7441 gcaatcagga tatgctgctg tcggtgctcg atctggaaaa aatgaccgtt gatgacatca
     7501 tggtgccgcg cagtgaaatt atcggtattg atatcaacga tgactggaaa tcgattctgc
     7561 gccaactctc ccactcacct cacgggcgca tcgtgctcta ccgtgattcg ctggacgacg
     7621 ccatcagtat gctgcgagta cgtgaagcct ggcggttgat gtcggagaaa aaagagttca
     7681 ccaaagaaac catgctgcgc gccgcggacg agatctattt tgtgccggaa ggtacgccgc
     7741 tcagcacgca gttggtaaag tttcagcgca acaaaaagaa agtcggcctg gtcgtcaacg
     7801 agtatggaga cattcagggg ctggtgacgg ttgaagatat tctggaagag attgtcggcg
     7861 atttcactac gtcgatgtcg ccaacacttg ccgaagaggt cacgccgcaa aacgacggtt
     7921 cggtgattat cgatggcacc gccaacgtgc gggaaatcaa caaagccttt aactggcatc
     7981 taccggaaga tgatgcccgc acggttaatg gcgtcattct tgaggcactg gaagagatcc
     8041 ctgtcgcagg cacccgcgtg cgtattggcg agtacgatat agatattctc gacgtacagg
     8101 acaatatgat taagcaggta aaagtttttc ctgtgaaacc gctgcgcgag agtgtggcgg
     8161 agtaacgaaa acggccctgc attcgcatgc agggccgtga attattacga aaacagaaat
     8221 tacgcttttg ctttcgctac agttaccatc gccgcacgaa tcgtacgacc attcagcgta
     8281 taacccttct gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga ttccaccatt
     8341 gcgatagcct gatgcacatt cgggtccagc ggcacgttag tttcggcgat cacttcaaca
     8401 ccaaacttac gcacaacatc cagcatcgac ttcagcgtca gctcaatgcc ttcaaccatc
     8461 gcagacatat ccgggttagc tttatcagcc acttccagcg cacgatccag gctatcaatc
     8521 accggcagca attcgttgat gaatttctcc agcgcgaatt tgtgggcttt ttcaatatcc
     8581 agttcagtac gtcgacgcag gttttccatt tcggctttta cacgcaaaat gccgtcacgt
     8641 tcacgggtct gggcttcagc cagctgagct tcgagattcg caattttttc atcgcgcgga
     8701 tccacctgct cagcagaagc ttctggctca actgcctcaa tctcttcgtg ctgatccatg
     8761 ataatttctt ccggggcttg cccctcaggc gttttctgtt ctttactact catgaatttc
     8821 tccgcgtttt tttcgcattc atctcgctaa cttcgcttat tatggggatc agtttcaggg
     8881 tttcaaggga agcactcaca ttgtcatcaa tcttcgcaac aaggacctcg gaaaaatgaa
     8941 taatcatttc aagtgtattg gcattgtggg acacccacgg caccctactg cactgacaac
     9001 acatgaaatg ctctaccgct ggctgtgcac aaaaggttac gaggtcatcg ttgagcaaca
     9061 aatcgctcac gaactgcaac tgaagaatgt gaaaactggc acgctcgcgg agattgggca
     9121 actggctgat ctcgcggtag tcgttggtgg cgacggtaat atgctgggcg cggcgcgcac
     9181 gctcgcccgt tacgatatta aagttattgg aatcaaccgt ggcaacctgg gtttcctgac
     9241 tgaccttgac cccgataacg cccagcaaca attagccgat gtgctggaag gtcactacat
     9301 cagcgagaaa cgttttttgc tggaagcgca agtctgtcag caagattgcc aaaagcgcat
     9361 cagcaccgcg ataaatgaag tggtacttca tccaggcaaa gtggcgcata tgatagagtt
     9421 cgaagtgtat atcgacgaga tctttgcgtt ttctcagcga tctgatgggc taattatttc
     9481 gacgccaaca ggctccaccg cctattccct ctctgcgggc ggtcctattc tgaccccctc
     9541 tctggatgcg attaccttgg tgcccatgtt cccgcatacg ttgtcagcac gaccactggt
     9601 cataaacagc agcagcacga tccgtctgcg tttttcgcat cgccgtaacg acctggaaat
     9661 cagttgcgac agccagatag cactgccgat tcaggaaggt gaagatgtcc tgattcgtcg
     9721 ctgtgattac catctgaatc tgattcatcc gaaagattac agttatttca acacattaag
     9781 caccaagctc ggctggtcaa aaaaattatt ctaattttac gccagcctct ttactgtata
     9841 taaaaccagt ttatactgta cacaataaca gtaatggttt ttcatacagg aaaacgacta
     9901 tgttggcaca actgaccatc agcaactttg ctatcgttcg tgagcttgag attgattttc
     9961 atagcggcat gaccgtaata actggcgaga ccggcgcggg taaatctatt gcaatagatg
    10021 ccctcggtct ttgtctcggt ggtcgcgctg aagccgacat ggtgcgtacc ggcgctgctc
    10081 gcgctgacct gtgcgcccgt ttttctctga aagatacgcc agcggctctg cgctggctgg
    10141 aagaaaacca gctagaagac gggcatgaat gtttgcttcg tcgcgtgatc agcagcgatg
    10201 gtcgctcccg tggtttcatc aacggtacag ctgttcctct gtcacaactg cgcgaactgg
    10261 gtcagttgct gattcagatc catggtcagc acgctcatca gttactcacc aaacccgagc
    10321 accaaaagtt cctgcttgat ggctatgcca atgaaacctc tctactccag gaaatgacag
    10381 cacgttatca gttgtggcat caaagctgcc gtgacctcgc gcatcatcaa cagttaagtc
    10441 aggaacgcgc tgcccgtgcg gaactgctgc aataccaatt aaaagaactt aacgaattta
    10501 atccgcagcc cggagagttt gaacaaatcg acgaagagta caaacgtctg gcgaacagcg
    10561 gtcaattgct gaccaccagc cagaatgcat tggcattaat ggccgacggt gaagacgcaa
    10621 acctgcaaag tcagctttac acggctaaac aactggtgag cgaattgatt ggcatggaca
    10681 gcaaactgtc cggcgtactt gatatgctgg aagaagctac catccagatt gctgaagcca
    10741 gcgatgaact gcgccactac tgcgatcgtc tggatctcga tcccaaccga ctatttgaac
    10801 ttgaacagcg catctcaaaa cagatttcgc tggcacgtaa acatcacgtc agccctgagg
    10861 cattgccaca gtattaccag tcgctactgg tagaacagca gcaactggac gatcaggccg
    10921 actcacaaga aacgcttgcg ctggcggtaa cgaaacatca tcagcaggca ctggaaatcg
    10981 cgcgcgcatt acaccaacaa cgccagcaat atgcagaaga acttgcacag ctgatcaccg
    11041 acagtatgca tgcgctctca atgccgcatg ggcagtttac gatcgatgtt aaatttgacg
    11101 agcatcacct gggcgctgac ggtgccgatc gtattgagtt tcgggtaacc accaacccag
    11161 gtcagccaat gcagcctatt gccaaagtcg catccggtgg tgaattgtcc cgcatcgcac
    11221 tggcaatcca ggtcatcacg gcgcgtaaaa tggaaacccc ggcactgatt tttgatgaag
    11281 tggatgtagg gattagcggt ccaacagcgg cagttgtcgg caaactgctg cgtcaactcg
    11341 gcgaatcaac tcaggtgatg tgtgttaccc acctgccaca agtcgcggga tgtggtcatc
    11401 aacactattt tgtcagcaaa gaaaccgatg gtgcgatgac agaaacgcat atgcaatccc
    11461 tgaataaaaa agcgcggtta caagagctgg cgcgcctgct tggtggcagt gaagtcacac
    11521 gtaatacact ggcgaatgcg aaagaactgc ttgcagcgta aacttttttc ctgcttcacg
    11581 gtcagagtaa acagcaaaac gccgtaagac cggaaagcaa aaggttttaa agtgatgaaa
    11641 ggtctattat catcggcata ttacagatga gccacgtact gctcgggccc gaaaaggaat
    11701 caaatcacta tgcgctgtaa aacgctgact gctgcagcag cagtactatt gatgttgacc
    11761 gcaggctgtt ccactctgga gcgagtggtt taccgtcctg acatcaacca ggggaactat
    11821 ctgaccgcta acgacgtatc caaaatacgt gttggcatga cgcaacaaca agttgcgtac
    11881 gcattgggta caccgctgat gtccgatcca tttggtacga atacctggtt ctatgtcttc
    11941 cgccagcaac caggtcatga aggtgtaacg cagcaaacgc tgacgctgac ctttaacagt
    12001 agcggtgtgt tgaccaatat tgataacaaa cctgcgctga gtggtaacta ataataaagt
    12061 tgctctcaaa gacgttaaaa aaggtgctca atgagcacct tttttctgtc tgttatttat
    12121 tcgctgattt ttctgctcgt tgcctgcgaa gctctttcgg atcggcaatg agagggcgat
    12181 aaatctccac ccgatcgcca tcatgcacac tatcgcttag ttttgccgga cggctgtaaa
    12241 tgccgacttt gtttttagtt aaatcgatat cggtacgcaa ttccagcaag ccactggcgc
    12301 gaatagcttc ttcaaccgtc gcgccctcct gcagcgtcac gcgctgcagg tactgcttgt
    12361 caggtagcgc ataagccacc tcaacggcaa ttttacctgg cactgtaaac ctctttcgca
    12421 cgaaccgtaa aagcctggac catattagac gccagctctt taaacacgcg accaaaggcg
    12481 agttcaatca acttattggt aaactcaaag tcgagatgaa actcgatacg acacgcctcc
    12541 tggctcagcg gcgtaaactt ccatccacca atcaatttct tgaacggccc atccaccaga
    12601 ttcataagaa tactttggtt actggtcaac tggttgcggg tagtaaacgt tttgctgatc
    12661 ccagccttag agacatctac cgccgcagtc atctgcccag gagtggactc cagaatccga
    12721 cttccggtac aacccggcaa aaactgagga taagactgaa cgtcattcac taactgatac
    12781 atttgctccg cgctgtaggg taccagtgcg gtccggctaa tctgaggcat aactatttcc
    12841 atcaacaaaa atccaacaaa taatatcatt tatcctgctg taaaaaaaac gctatcccgg
    12901 cgctgggtaa catcgggttc atgctaagat agagccttgt cccccgcagg attgatatgg
    12961 ggtgttttcg atttcagatt accgatgatt cacgacgctt atgacgaaga aaaaagcaca
    13021 taaacctggt tcagcgacca tcgcgcttaa caagcgcgcc cgtcacgaat actttatcga
    13081 agaagagttc gaagcgggac ttgccctgca aggctgggaa gttaaatccc tgcgcgcagg
    13141 aaaagccaat atcagcgaca gctacgtcct tctgcgtgac ggagaggcat ttctgtttgg
    13201 cgctaacatc acgccaatgg ccgtggcctc cacgcatgtg gtgtgcgatc ctacccgtac
    13261 ccgcaagtta cttctcaacc agcgcgaact ggactcattg tacggtcgcg tcaatcgaga
    13321 aggctatacc gtagtggcgc tctccctgta ctggaaaaat gcctggtgca aagtgaaaat
    13381 cggcgtcgcc aaaggtaaaa aacagcacga taaacgttca gatatcaaag aacgcgaatg
    13441 gcaggtggat aaagcacgta tcatgaaaaa cgcccaccgt taaacctgca ctccaattat
    13501 tgaccagttc ctcaccgcgc ctccctctcc ggcggcgcga atgaacatct tattggctat
    13561 cacatccgac acaaatgttg ccatcccatt gcttaatcga ataaaaatca ggctacatgg
    13621 gtgctaaatc tttaacgata acgccattga ggctggtcat ggcgctcata aatctggtat
    13681 acttaccttt acacattggg gctgattctg gattcgacgg gatttgcgaa acccaaggtg
    13741 catgccgagg ggcggttggc ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa
    13801 ctacgcttta gcagcttaat aacctgctta gagccctctc tccctagcct ccgctcttag
    13861 gacggggatc aagagaggtc aaacccaaaa gagatcgcgt ggaagccctg cctggggttg
    13921 aagcgttaaa acttaatcag gctagtttgt tagtggcgtg tccgtccgca gctggcaagc
    13981 gaatgtaaag actgactaag catgtagtac cgaggatgta ggaatttcgg acgcgggttc
    14041 aactcccgcc agctccacca aaattctcca tcggtgatta ccagagtcat ccgatgaagt
    14101 cctaagagcc cgcacggcgc aagccctgcg ggcttttttg tgccctcaat ctgtcccgcg
    14161 aagtccgaag agaactaatt aaatccgaac cttttaggcc cattgatagg cccaacgaaa
    14221 agctctattg ttttcgttgg gcctaaacgc atggagattc cccatggcaa gaaaaaccaa
    14281 gccgttaacc gatacggaaa tcaaagccgc caaacctaaa gatgccgatt accagctgta
    14341 tgatggggac gggcttactc tattaatcaa gtccagtggt agtaagctct ggcagttccg
    14401 ttactatcga ccgctgacaa aacagcgaac caaacagagc ttcggtgcct accctgcagt
    14461 ctccctttct gatgcgcgta aactcagagc tgaatctcga gttttgttgg cgaaagacat
    14521 tgatcctcag gatcatcaga aagaacaggt aagaaattct caagaggcta aaactaacac
    14581 tttcctgtta gttgccgagc gttggtggaa tgtgaagaaa accagcgtaa cagaggacta
    14641 tgccgacgat atctggcgct cgcttgagag agatgttttc ccagcaatcg gtgatatcag
    14701 tgtcactgag attaaggctc atactctggt taaagcagtc agccggttca ggccagaggt
    14761 gcattagaga cagtccgacg cctttgtcag cgtattaacg aggtcatgat ttatgcgcag
    14821 aacacaggct tgattgatgc agttcccagt gtaaatatcg gaaaagcgtt tgagaaaccg
    14881 caaaagaaaa acatgccaag catacgcccg gatcaacttc cacagctaat gcagactatg
    14941 cgtacggcaa gtatcaactt gtccacaaga tgtctattca tgtggcaact tctcaccatc
    15001 acccgtcctg ccgaagctgc tgaggctcga tgggatgaga tcgattttga ggctaacgaa
    15061 tggaaaattc ctgcagcccg aatgaagatg aaccgagacc atacggttcc actatccgat
    15121 gaggctcttt ctgtactgca aatgatgaag tcacttagtg gtggccgaga atttattttt
    15181 cccagtcgca ttaagccgac ccagccaatg aacagccaaa cagttaatgc agcccttaag
    15241 cgtgctggct taggaggcgt tctcgtatca catggtttac gttctatcgc tagtacggcg
    15301 ctcaatgagg aaggatttcc gcctgatgtt attgaggctg cacttgctca tgtggataag
    15361 aatgaggttc gtcgcgctta caatcgaagt gactatcttg agcaacgacg accgatgatg
    15421 cagtggtggg ctgattttgt taaagcagca gataacggca gcattatgaa tagtagg
//
LOCUS       DAJSKJ010000088        15448 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000088 DAJSKJ010000000
VERSION     DAJSKJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.088"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..2541)
                     /locus_tag="ON345_002363"
     CDS             complement(<1..2541)
                     /locus_tag="ON345_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR3072414.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQHSLRTCPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKR"
     gene            2659..3852
                     /locus_tag="ON345_002364"
     CDS             2659..3852
                     /locus_tag="ON345_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3072415.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPGNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(3916..5169)
                     /gene="glyA"
                     /locus_tag="ON345_002365"
     CDS             complement(3916..5169)
                     /gene="glyA"
                     /locus_tag="ON345_002365"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR3072416.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            5497..6687
                     /gene="hmpA"
                     /locus_tag="ON345_002366"
     CDS             5497..6687
                     /gene="hmpA"
                     /locus_tag="ON345_002366"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR3072417.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(6732..7070)
                     /gene="glnB"
                     /locus_tag="ON345_002367"
     CDS             complement(6732..7070)
                     /gene="glnB"
                     /locus_tag="ON345_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR3072418.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7131..8465)
                     /gene="glrR"
                     /locus_tag="ON345_002368"
     CDS             complement(7131..8465)
                     /gene="glrR"
                     /locus_tag="ON345_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR3072419.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(8455..9168)
                     /gene="qseG"
                     /locus_tag="ON345_002369"
     CDS             complement(8455..9168)
                     /gene="qseG"
                     /locus_tag="ON345_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR3072420.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(9333..10760)
                     /gene="qseE"
                     /locus_tag="ON345_002370"
     CDS             complement(9333..10760)
                     /gene="qseE"
                     /locus_tag="ON345_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR3072421.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(11336..15223)
                     /gene="purL"
                     /locus_tag="ON345_002371"
                     /gene_synonym="purI"
     CDS             complement(11336..15223)
                     /gene="purL"
                     /locus_tag="ON345_002371"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR3072422.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         3614 a         4219 c         4018 g         3597 t
ORIGIN      
        1 gcgtttcagc gcaatttcca gattattttc cagacgcgcc agtcgtttct ccggtgtggc
       61 tccgcttaac ttatccagca aatcccgttc gaaaagcaga cgtgcatcct gcggcgcaag
      121 ctgataagca ttatcaagat aacgcgcggc cagctcataa tcgtgttgct tattccacgc
      181 atggatcgct aacccgcgcc agccgtcggc aaactccggc gacatctcta cgcaacgttg
      241 ccataaggca atggctttgt tgtagctacg tttgttgtag tagaagcagg ccagtaaatg
      301 gcgagcaaac cagcactctt caatactctc cagcgccgcc acttcttcca acgtattcgg
      361 gaaacggaca aactgcggga agacatcaat ggcttttgcg accagttcgc cacgttcggc
      421 tttcggcagc aagctggctt gcaggtaaag cggcagcgtg cgctggcagt ccagtgcgtt
      481 cagcatctct gccgccaggg tgggcattcc ccagttaatc agttgcccgg cggtcatcag
      541 ggcgttaacg tcgcgtccct gacacagacc gcgccactgc gcgagagctg attcgctacg
      601 accatcgaac cagttcagcc accacagagt ggcgttcagc ggataatcgc gcagcagttt
      661 ctcgcgctgc aaacgcgcgt tgtcctgacg accacttaac accagcagca gattatgcag
      721 gcaaagcact tcctgattgg ttgggcaggt gcgaagactg tgttggcaaa aatccagacc
      781 cgcgtcgaag tttccattac gcgccgccag tcgtgccaga ccataatagc cgcctgcttt
      841 actattgccg ctccagaccg cacgccagaa atcctcttcg gcttgttgat attgtccctg
      901 acgttcgtaa gcactggcgc gaatcaaact ggcctgtccg cactgcggat ttttgttcag
      961 cgcatgtgcg cgtttcagag cctgagtggc atacgccacc gcttgcggga aatcggcgcg
     1021 gttatattcc agcatcgcca gcgccaggtt acagcggtaa tccagcggat ccagcgccac
     1081 gccgcgcagg tagtaatcga acggtgaacg gctggcgtga tgatattgct ccagatgctg
     1141 accgataaac caggcttcat ctgtactggt aatgtcttgt gctgccagtg gcgctttggc
     1201 gacgtccggc aacggcaact cctgcggctg atgttcctga taactcagta caatattgcc
     1261 gtcggcatcg gagagctcaa tggtcagcct atcaggattg ataccgtgca acacgccctg
     1321 gatggcggtc gcaggcatca gtgccacggc atcatcaagt aacgcgttgc atttgccgat
     1381 ttcgcggatc gccaggcggt atccgttcaa cggagagatg gcatacagcc cccactcaat
     1441 cccccgctta ctacgctgga gttttatcac cgcatcgcgg gaggcatttt gcaccatgcc
     1501 caaagaatga taaggcagga aatactgctc aaaacgcttc tcttcgtaag catcaagcca
     1561 ggtaaaatcg ggctggttat cggcaaaaat accggtcatc agttcgatat acgggccgtt
     1621 attgtcggtc agactcttat cccacgcctg gccaaattca ctgtgtcccc aactccactg
     1681 ttttttacct ggcgcaatat ggtggttggc aacgtgcagc aaaccgccat cttcatcgtg
     1741 acaccacgcg ccgacaaaat cgtactgcga tttttcggcc atatatgagg ttggaacagg
     1801 cacattttta tagcgagaaa tgtccactcc ggcggagtag tccactttgt agtaagtgcc
     1861 ggtggcgatg gggaaagcgg agacggcccg tttgccgtga tcaaacaccg cagtcacatc
     1921 cggtgggaaa acgctctgat gcccttcccc ccctttcact gccgggttgg cccaccacaa
     1981 gaaatgacgc ggcgtggcgt taccgttata gacgcggctg gcgatttcca gcgccgcccg
     2041 gtcagggcgc agggtgaaac ctgtcatcac ctgtaaacca tgcatcggct ccgtttcgcc
     2101 tacccacacc gtctgtgcac cgtcttcatg ggcttcgagg gtgaaatcaa cgggcataaa
     2161 ggtggtcggg cgatgatgtt gcggccagtt aaactcaatc ccaccggaaa tccacggccc
     2221 cagcagcccc accagcgcag gtttaatgac ttcattgtga taaacaaaat cgcgctgttt
     2281 cactttatcc cacgcgcgat gcacccgacc gcccagttcc ggcaggatca tcactttgat
     2341 gtagtcgttt tccagccaca ccgcctgcca ggatttcagg gttttctgct cgctcagcgt
     2401 atcggtcacg ccgtagggat aaaccgcgcc ggacgatccc tgataaacac gattttccag
     2461 gaacatggga tgtatatcct gcggcccggt ttcataggtc gggatctcaa cgcgctcttg
     2521 ccacactttt actggagtca tggtgccctc aatattaaca agacatactg aattaaaaga
     2581 tttgtggcag tgtattgaac aatctggcaa tgttttcgcg gaataatcac gcaattaact
     2641 aaacaaggtt tagtgaagat gagagcctgc attaacaatc aacagattcg ccaccataac
     2701 aaatgcgtga ttctggaact gctgtaccgg caaaagcgcg ccaataaatc aacgctggcc
     2761 cggctggcgc aaatttcgat tccggcagtc agtaatattt tgcaggaact ggaaagcgaa
     2821 aaacgggtgg tgaatattga cgatgaaagc cagacgcgcg ggcatagtag cggtacatgg
     2881 ctgattgcgc cggaaggtga ctggacgctg tgcctgaacg tgacgcccac cagtattgag
     2941 tgtcaggttg ctaatgcttg tttaagtccg aaaggtgaat ttgagtattt acagattgat
     3001 gcaccgacac cgcaggcact gctgtccgaa atcgaaaaat gctggcatcg tcatcgcaaa
     3061 ctgtggccgg gccgcaccat caatctggcg ctggcaatcc acgggcaggt tgatccggtg
     3121 accggcgtat cgcaaaccat gccgcaagcg ccgtggacaa cgccggttga ggtgaagtat
     3181 ctgctggaag agaagctggg cattcgggtg atggtcgata atgactgcgt gatgctggcg
     3241 ctggcggaga aatggcaaaa taattcgcag gaacgggatt tctgcgtgat caacgttgat
     3301 tacggcattg gctcgtcgtt cgtgattaac gagcaaattt atcgcggcag tttgtatggt
     3361 agcggacaga ttggtcacac catcgttaat ccggatggcg tcgtctgcga ctgtggacgt
     3421 tacggctgcc tggaaaccgt cgcctcgtta agtgcattaa aaaaacaggc gcgggtatgg
     3481 ctaaaatcac aaccgggtaa tactcagctt gatcctgaaa aactgacaac cgcgcagtta
     3541 atcgctgcct ggcagagtgg agaaccgtgg atcaccagct gggttgatcg ctctgccaat
     3601 gccattggtt tgagtctgta taacttcctc aacatcctca atattaatca gatttggttg
     3661 tacggtcgca gttgtgcctt tggtgagaac tggcttaata ctattattcg ccagacagga
     3721 tttaacccgt tcgaccgcga cgaaggaccg agcgtgaaag cgacgcaaat tggcttcggg
     3781 caattaagcc gcgcacaaca ggtgctggga attggctatt tgtatgttga ggcgcagtta
     3841 cgacagattt gatggcgcga taacgtagaa aggcttcccg aaggaagcct tttttcaaca
     3901 gattaaccgt ttcgcttatg cgtaaaccgg gtaacgtgcg cagatgtcga gaactttacc
     3961 tttgatgcgc tcgataacgg cttcatcatt gatgctgtcc agcacgtcac acatccagcc
     4021 agccagttct ttcgcttctg cttctttgaa gccgcgacgc gtaattgccg gagtacccac
     4081 gcgaataccg gaggtcacaa acgggctctt cggatcgttc ggtacgctgt ttttgttgac
     4141 ggtgatgtta gcacggccca gagcggcgtc tgcttcttta ccggtcaggt ttttatcaac
     4201 cagatcaacc aggaacaggt ggttatcagt gccgccggaa accactttgt agccgcgctc
     4261 gaggaacact tctaccatcg ctttagcgtt tttcgcgacc tgctgctggt aagttttgaa
     4321 ctcaggctcc atcgcttctt tcagagcaac cgctttaccg gcgattacgt gcatcaacgg
     4381 accgccctga ccaccaggga aaacggcaga gttcagtttt ttgtacagct cttcgctacc
     4441 acctttcgcc aggatcaggc cgccgcgcgg acccgccagg gttttgtgag tggtggtagt
     4501 aacaacgtga gcatgaggaa ccgggttcgg gtagacgcca gcagcaacca ggcccgcaac
     4561 gtgcgccata tcaacgaaca ggtaagcacc gatgctgtca gcgatttcac gcattttcgc
     4621 ccagtccacc acgccggaat atgcagagaa accaccgata atcattttcg gcttgtgttc
     4681 tttggcttgt ttttccagat cggcgtagtc gatatgaccg gtagcatcga taccgtaagg
     4741 aacgatgttg tacagtttac cggagaagtt aaccggagaa ccgtgagtca ggtgaccgcc
     4801 atgcgccagg ttcataccca gaacggtatc acctggttcc agcagcgcgg tgtagaccgc
     4861 aaagttagcc tgggagccgg agtgcggctg gacgttagcg tagtcagcgc cgaacagttc
     4921 tttcgcacga tcgatcgcca gttgttcaac gatatcaaca tactcgcaac cgccgtagta
     4981 gcgtttgccc ggataacctt cagcatattt gttggtcagc tgagaaccct gcgcctgcat
     5041 tacgcgcggg ctggtgtagt tttcggaggc gatcagttcg atgtgctctt cctgacgtac
     5101 tttttcctgc tccatagcct gccacagttc ggcatcataa tcggcaatgt tcatttcacg
     5161 ctttaacatc cgcatctcct gactcagcta acaataaaat ttttggcctt tataggcggt
     5221 cctgttggac aacggcgaac agtataaccg aatcattgtg cgataacagg tcttgacaaa
     5281 ggaatttacg caaacgatta ccttcaggct acgcaaggct ttggagaata aagagcttgc
     5341 aaccggaaac ggatttcttt tcaggtttgt gatgcaaatt tttcacttca tcacattctt
     5401 tctaaaaaac accaaagaac catttacatt gcagggctat tttttataag atgcatttga
     5461 gatacatcaa ttaagatgca aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
     5521 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
     5581 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
     5641 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
     5701 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
     5761 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
     5821 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
     5881 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
     5941 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
     6001 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
     6061 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
     6121 actcgcaaac cggatggcaa aggctatcgt attgcggtga aacgcgaaga gggtgggcag
     6181 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
     6241 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctgccggt
     6301 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
     6361 caagtgaact ggttccatgc ggcagaaaat ggcgatgttc acgcctttgc cgatgaagtt
     6421 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
     6481 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
     6541 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
     6601 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
     6661 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
     6721 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccgt
     6781 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
     6841 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
     6901 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
     6961 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
     7021 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
     7081 gaaaaggtcg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
     7141 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
     7201 cccgccattc tcgccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
     7261 tagttgagtt caaactgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
     7321 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
     7381 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc aactcgcggt catcaggcgt
     7441 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
     7501 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
     7561 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
     7621 tttggtagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
     7681 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
     7741 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
     7801 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
     7861 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
     7921 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
     7981 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
     8041 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
     8101 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
     8161 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
     8221 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
     8281 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
     8341 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
     8401 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
     8461 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
     8521 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
     8581 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
     8641 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
     8701 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
     8761 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
     8821 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
     8881 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
     8941 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
     9001 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
     9061 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
     9121 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
     9181 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
     9241 aaccggagcg tacatatcgt acgtgaagat ttcgagcact gcccggggcc gaactgacaa
     9301 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
     9361 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
     9421 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
     9481 cgctggtggc ttccctgaaa aaagggttcg aagatcatgg cgcgttcctc ttgcggaatg
     9541 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
     9601 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
     9661 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
     9721 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
     9781 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
     9841 tgttcgatca gtttttgcaa attgcggctg ctgctatcaa gaatgctcac cacctctttt
     9901 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
     9961 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    10021 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    10081 ctcggtccac tgaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    10141 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    10201 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    10261 tcacgctgaa gttgctgccc acgagaaaac accactgtgc gcgtggcctg caccatttcg
    10321 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    10381 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    10441 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    10501 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    10561 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    10621 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    10681 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    10741 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    10801 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    10861 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    10921 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    10981 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    11041 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    11101 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    11161 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    11221 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    11281 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    11341 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    11401 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    11461 tgactcgacc actttcagtc gtaaccgacg taataccgtt cggtgaaccg ttcgggttag
    11521 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    11581 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    11641 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    11701 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtattgcgc acaaaacgtg
    11761 gccacaactc actacctggg atcagttcgc gcaggttaga catcatctgg caaccgttac
    11821 atacccccag cgccagcgtt tgcggacggt ggaagaaggt tgcaaactca tcgcgtacac
    11881 ggtcattgaa caggattgac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    11941 agaaaccacc gcacgcgacc agggcgtgga aatcttccag gcccgtgcgt ccggtcagca
    12001 ggtcactcat atgcacgtcg atagcatcaa agcctgcacg gtggaaagct gccgccattt
    12061 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    12121 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    12181 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    12241 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtggtg cggctttcgc
    12301 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccta
    12361 cataatggac acaatcagca agcccatgct gtgccagtac ggactcaacc gcttcacggt
    12421 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    12481 cgtcgcccag agaggcaata tccgcgttaa tgccgcaatg accagcaaag gccatttccg
    12541 ccagcgttac cagcaggccg ccatcagagc ggtcgtggta tgcgagcagc ttacgctgtg
    12601 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg cgcgacgtcg cgtacatctg
    12661 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    12721 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtagaaagc tgtggcgtga
    12781 tggtgtgacg cacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    12841 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    12901 ccaccgggat cgtcaggccc agcgccggac aaagctcttc gcccacggct ttaacggctt
    12961 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    13021 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    13081 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    13141 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    13201 cctgccacgg acccaccatc tgatcgcgcg ctaccatgcc agttacgctg cggtcaccga
    13261 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    13321 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    13381 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    13441 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    13501 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    13561 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    13621 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgcccgccg tcgctcacca
    13681 gttccggcat cgcgttagaa agaccgccag cgccgacgtc gtggataaac aggattgggt
    13741 tggcatcacc aagctgccag caacggtcga tcacttcctg gcagcgacgc tccatctccg
    13801 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacct gacgccatgg
    13861 aagacgccgc tccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    13921 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    13981 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccgttgtgg ctgttcactt
    14041 tttcttcata agtacggaag tagccattca gtgccggacg accaaattcg ttgttaaacg
    14101 ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc ggtgacaatg cgctcaggct
    14161 taccgaaatc ttcttcccac ggctgttcaa agccaggaat tcgcaggttg gaaacggaga
    14221 aaccaaccag accagccttc ggcttcgcac cgcgtccggt ggcgccttca tcacgaattt
    14281 cgccgccgga acccgtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    14341 tttcgacttt catcagaata tgcgccggtt cctgatggaa atcgtagcgg cccgtttcgt
    14401 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    14461 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    14521 tcggctgctg ttcaccgtcg ataatccagt cggcattaaa aattttgtgg cggcagtgct
    14581 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    14641 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    14701 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgctggta accggagtcg
    14761 gttgatggtg ggcaaacaac tgctctgcat catctaaagc aaaaaagacc gtttccatca
    14821 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    14881 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    14941 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    15001 ggagtttgcc ttgcggggcg tggctggcga gtgccgggcc atatttcagc aggcgttcaa
    15061 gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg
    15121 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    15181 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    15241 agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgtgctga
    15301 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    15361 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    15421 cctttcacta ttattacgtt ttttcaag
//
LOCUS       DAJSKJ010000089        15412 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000089 DAJSKJ010000000
VERSION     DAJSKJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.089"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(131..937)
                     /gene="trpA"
                     /locus_tag="ON345_002372"
     CDS             complement(131..937)
                     /gene="trpA"
                     /locus_tag="ON345_002372"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR3072423.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRASLPLNHLIAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(937..2130)
                     /gene="trpB"
                     /locus_tag="ON345_002373"
     CDS             complement(937..2130)
                     /gene="trpB"
                     /locus_tag="ON345_002373"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR3072424.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETTHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2142..3503)
                     /gene="trpCF"
                     /locus_tag="ON345_002374"
     CDS             complement(2142..3503)
                     /gene="trpCF"
                     /locus_tag="ON345_002374"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR3072425.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3504..5099)
                     /gene="trpD"
                     /locus_tag="ON345_002375"
     CDS             complement(3504..5099)
                     /gene="trpD"
                     /locus_tag="ON345_002375"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR3072426.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEMAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGHQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5099..6661)
                     /gene="trpE"
                     /locus_tag="ON345_002376"
     CDS             complement(5099..6661)
                     /gene="trpE"
                     /locus_tag="ON345_002376"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR3072427.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6683..6776)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6726..6797)
                     /gene="trpL"
                     /locus_tag="ON345_002377"
     CDS             complement(6726..6797)
                     /gene="trpL"
                     /locus_tag="ON345_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR3072428.1"
                     /translation="MKAIFVLKGWWRTSSNGQCIHHA"
     gene            6935..7816
                     /gene="yciV"
                     /locus_tag="ON345_002378"
     CDS             6935..7816
                     /gene="yciV"
                     /locus_tag="ON345_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR3072429.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7813..8433
                     /locus_tag="ON345_002379"
     CDS             7813..8433
                     /locus_tag="ON345_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR3072430.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8534..9406
                     /gene="rluB"
                     /locus_tag="ON345_002380"
     CDS             8534..9406
                     /gene="rluB"
                     /locus_tag="ON345_002380"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR3072431.1"
                     /translation="MSEKLQKVLARAGHGSRREIETIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9446..10036)
                     /gene="cobO"
                     /locus_tag="ON345_002381"
     CDS             complement(9446..10036)
                     /gene="cobO"
                     /locus_tag="ON345_002381"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR3072432.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(10033..10791)
                     /locus_tag="ON345_002382"
     CDS             complement(10033..10791)
                     /locus_tag="ON345_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR3072433.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11011..12060
                     /gene="sohB"
                     /locus_tag="ON345_002383"
     CDS             11011..12060
                     /gene="sohB"
                     /locus_tag="ON345_002383"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR3072434.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12096..12347)
                     /locus_tag="ON345_002384"
     CDS             complement(12096..12347)
                     /locus_tag="ON345_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR3072435.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12727..15324
                     /gene="topA"
                     /locus_tag="ON345_002385"
     CDS             12727..15324
                     /gene="topA"
                     /locus_tag="ON345_002385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR3072436.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3747 a         3924 c         4128 g         3613 t
ORIGIN      
        1 aaatgacgta agttgacgtt cgaccggggt aagcgaaacg gtaaaaagat aaatattaaa
       61 tgaatttagg atttttccgg cttcattaaa gaaagttaaa atgccgccag cggaactggc
      121 ggctgtggga ttaactgcgc gtcgccgctt tcatcggttg tacaaaaact ttcagtgccg
      181 ccagcatttt ctctggctca ttaatatgtt gctcgatgat tttaacaatg gccgaaccag
      241 aaatcgcgcc tgcagctcct gcatcaatcg ctgcttttac ctgatccggg gcggaaatac
      301 caaacccctg caatggaggt gcagcgttgt actctttcag cttcgcaatc agatgattga
      361 ggggtaacga ggcgcggttt tctgcgccgg tcacgcccgc tcgtgacagc aaatatgtgt
      421 aaccacgacc gtaagaggct atctggcgca gcaggtcgtc atcggcattt ggcgggcaga
      481 tgaagatagg tgcgacatta tgacgcaacg cggcctggcg gaagggcgcg gactcttcaa
      541 ctggcacatc ggcaaccagc accgaatcga cgccgacttt ttcgcactgg gcataaaact
      601 catcaatgcc tttgttaaac accagattgg catacatcaa caggccaatg ggaatggtcg
      661 ggtgtttctg gcgaatcagt gccagcattt caaaacattg tgccggagtc acacctgccg
      721 caaaggcgcg cagagtggcg ttttgaatgg tcgggccatc cgccagtggg tcggagaagg
      781 ggatacctaa ctccagcgcg tcagcaccgg cttcaattag cgtatcgata attttcaatg
      841 actgctcaat gcccggatca ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct
      901 ccttcaactg ggcaaacaga gattcgtagc gttccatcag atttcccctc gtgctttcaa
      961 aatatcgtga acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag
     1021 ctgctcttta tccgggtttt cgcgcatcat tttcaacgca tgggccaggg cgtgggagga
     1081 ttccagcgcc gggatgatcc cttcgtgcag gcacagcgtt ttgaaggctt caagggcttc
     1141 atcatcggta atagacacgt aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg
     1201 gccgacagac gggaaatcca gtccggcgga gatggagtaa gattcttcaa tctgcccgtc
     1261 ttcggtttgc atcatcggcg ctttcatacc gaaatagata cccacgcgac catgttttag
     1321 cggtgcgccg tgctcgccag tttcgatacc gtgaccacct ggctccacac caatcaggcc
     1381 gacgttggtt tcattgatga aatcagcaaa catgccgatg gcattcgaac cgccgccaac
     1441 acaggcgata acggcatccg gcaggcgacc ttctctttcc agaatctgcg ctttggtttc
     1501 ttcgccaatc atccgctgaa actcacgcac aatggtcgga taaggatgcg ggccagctgc
     1561 ggtgcccagc atatagtgcg tggtttcgta actaccggac cagtcgcgca gcgcctcgtt
     1621 acaggcatct ttcagcgtcg cggaaccgct atgcaccggg atcacttccg cacccattaa
     1681 gcgcatacga aaaacgttag gcgactggcg ttcaacgtct ttggcaccca tataaatacg
     1741 gcatttcagg ccgagcaggg cgctggcaag ggccgacgcc acgccatgct gaccggcacc
     1801 ggtttcggcg atgatttcgg ttttacccat ccgcttcgcc agtaaagcct gaccgagcac
     1861 ctggttagtt ttatgcgcgc cgccgtgcag caaatcttca cgcttgagat acagcgtggt
     1921 gttcgtcccg gctgtaatgt tctggcattt ggtcagcgcg gttggacgcc cggcatagtt
     1981 tttcagcagg tcgttgaact gagcctgaaa ttcaggatct ttttgcgcac tgacaaaagc
     2041 ttcttccaac tggcgcagag caggcatcag gatttgtggc acgtacatgc cgccaaactc
     2101 accaaaatag gggttaagta atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc
     2161 tggaaaaccg aggccaaaag acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa
     2221 ttaaaatcaa gtccggcgca gccggtttgt gccgcttcca cgcagttatc tgcgcctaag
     2281 ccccccgcca gcagaacgtt gccaagcgat tgaccattta atagtgacca gtcaaaacgt
     2341 tgcccgcttc caccctggcc gttgtctaaa acatatttat caacgtgctg aagctcgcgg
     2401 gcgggcaggg tttcaccgac gcttaatgct ttccagatgg caacgtgtgc tggcagagct
     2461 tcacgcagcg tatcgatata cagctgatct tcattaccat gcagttgcac tgccgccagc
     2521 gataacacct tagctttgtc caccacatcg gcaatatcgt gattgcggaa cacgccaaca
     2581 tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt caacgttgac gcaacgcggt
     2641 gatgtcgcaa ccaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc tgctttagca
     2701 tcttgcccac gcgtcagacc acatacttta ttctcaccca gcaacacccg gcgcacggcg
     2761 gcgtgcaaat cgtcatgggc catcaacgcc gaaccaatca gaaaaccgtt agcgaagtgg
     2821 cttaactcgc gcacctgagc gtaagtattg atgccggatt cgctgattac cgtcacgttg
     2881 tgccccagtt tcggcgcaag ctcgcgggta cggttgagat caatcgacaa atcacgcaga
     2941 tcgcggttgt tgatgccaac gacctttgcc cccaatgcaa tggcacgctc cagttcctct
     3001 tcattactga cttcggtcag cacacccatc tccagactgt gggcgacggc ggcaagctgg
     3061 cgatattgtt cgtcatccag tactgaaagc attaataagc aggcatcggc ctggtaatag
     3121 cgcgccagat agatctggta aggatcgata atgaagtctt tacataaaat cggctgcggg
     3181 gcgatttggc tgacgatggg gaggaaatca aagctcccct gaaaatattt ctcatcagtc
     3241 agcaccgaaa ttgccgaagc gtaatgttta taaatggcgg caatgcgtgc cggatcgaaa
     3301 tcatcacgga tcacgccttt tgacggcgac gcttttttac actccagaat aaacgccgtg
     3361 cgtgcgccct gtagcgcatc ataaaaatgt cgcgtgctcg gctgaacctc attctgaaaa
     3421 ctggccagcg gttgctgctg tttgcgggtt tctacccaaa tcgccttgtc tgcgacgatt
     3481 ttcgctaaaa cggtttgcat catttaccct cgtgccgcca gcgcggtaac tctgtcgtaa
     3541 gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg
     3601 ccatgcaggc gcattaacat ggcgacgttc gcagcgacgg ctgcttcatg ggcggcgtcg
     3661 cctttacctt gtaacaaacg tgttaaaatg tcacggtttt cttccggtgt tccgcctgcc
     3721 agttgctcct ggtggtaggg tgtcaggcca aaatcttctg cagttagctg atagctctta
     3781 atttcaccgt catgcagttc ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc
     3841 ccgccgctgt gcaccaccgc cgcgcgttga tgccccagca cgcgcaaggt ttcggcaatc
     3901 ggcagcacca gttccggact ataaacacca attaacgcca gcggcggatg cgccgggtta
     3961 atcaacggcc ccagcacatt gaacagggtg cgggttttca gttgctggcg aaccggcatc
     4021 gcgtggcgga atccggtgtg atacttcggc gcaaagagga aacatacacc taactcatcc
     4081 agcgcctggc gcgatttatc ggcgttcata tcaagattaa taccgaacgc cgccagcaga
     4141 tcggacgaac cagatttact ggagacgcta cggttgccgt gtttcgccac tttcagccca
     4201 caggccgcgg cgacaaacgc actggcggta gaaatattga tactgttgct gccgtcaccg
     4261 ccagtaccga cgatatcagc aaacagataa tccgggcgcg ggaacggcgc ggcgttttcc
     4321 agtagcgcgg ttgctgctcc ggccatctcg ttcgggtgct caccgcgaat tttcatgctc
     4381 accagcgccg ccgccagttg ttccggcttc agctcgccac gcaccaccgc tgaaaacagc
     4441 tggtggcttt cttgttggct aagcgtctgc gcctgataca gtttttccag aatcggttgc
     4501 agcgtgttgg ctggctctag tttctgctgc gcccaggcca gcgtttgttc cagcaggcga
     4561 gcgccctggg tggtgagaat ggattccgga tggaactgga atccacaaac gcgatccgca
     4621 tcgtgacgta ctgccatcac catgccatta aaatgggcgt tgatggttaa accggccgga
     4681 atgttactgc caaccagcga gtgataacgc gccaccggca gcgggtttgt taatccggca
     4741 aacatcgcct gaccgtcatg ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc
     4801 tgaccgacat accccccata agcttcgaca atcgcctgat gaccgaggca aatgccaata
     4861 atgggcagct tgccacgcaa gcgggtgagg agttccggca tacaaccggc ttcgctcggc
     4921 acaccggggc caggagaaag catcagtacc ggattgctca tcgtcgccag gcgttcaatt
     4981 aaggtctgcg ccggaatatg gttgcggtaa atcaccacat tatgcccatt gctgcgcaac
     5041 tgatctgcca ggttgtacgt aaaagagtcg atattatcga gcagcagaat gtcagccatc
     5101 agaaagtctc ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg cgggctttat
     5161 tacgggtttc gtcggcttcc gactgcggaa cagaatcaag gactacgcca gcaccggctt
     5221 gcacggtggc gataccgttt tccaccagcg ccgagcggat cacaatgcag gtgtcgagat
     5281 cgccatgcgc ggtgaaataa cctaccgcgc cgccgtagct gccgcggcga cgaccttccg
     5341 cctcggcaat taactgcatg gcgcgtactt tcggcgcacc gcttaacgtc cccatattca
     5401 tacaggcgcg gtaagcgtgc agggcgtcga gatcgtggcg cagctcaccg accacgcggg
     5461 agaccaggtg catcacgtaa gagtaacggt caactttggt aagatcggcg acgtagcggc
     5521 tgccgggggt gcaaatgcgt gccagatcat tacgggcgag atccaccagc atcagatgtt
     5581 cagaaagctc tttatgatcg gtacgcattt ccagttcgat gcggctgtcg aggtctctgt
     5641 ccagcgagcc atcggcacga cgaccgcgtg gacgtgttcc ggcaatcggg tagatctcaa
     5701 tctggcggct ggtggcgtca tacttgagcg aactttccgg cgacgcgcca aacagggtga
     5761 aatcattatc ctgcataaaa aacatgtacg ggctgggatt actctttttc agcacgtaat
     5821 aggccgccag cggtgacggg cagggcagag agaaacggcg agatggcacc acctggaaaa
     5881 tttctccagc gcgaatcgct ttttgcaaca aacgcactac gccaccgaac tcttcatcgc
     5941 tctgattaca ttcacaacgc atatgcggca cggaaaccac tggcagcggc ggcgcggctt
     6001 cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag acgttgtttt tcttcttcat
     6061 tcggagcaaa caggctggcc tgaatacggg tgcttttttt ctgatggtca atcaccatca
     6121 gagtttcagc gagataaaaa cagaaatcag ggcagttatt ttccgctgac agttgcggta
     6181 aatcttcaaa tcccgccaca aggtcataag agaacaggcc gccgaagaac attgcttctc
     6241 gttcttcctt cggtacattc aacagattct gcaataaacg gaaagcgtca aaaaccgaaa
     6301 gggagcataa gcgagcgtct tcatccagca gtggactgac aggggggaag cgtagcacac
     6361 ggcagtttgg taattgttca ttttccacac ccgcaggcag ggcgttatcc agtagtgtca
     6421 gcagggcttc gccgttgccg gaaagtgcct ggattgtgac agtgtcacct aaagctgtaa
     6481 tgcgcagcgc actgtctacc agcagcaggc tttttaaatc atctttgctg tcgatatctg
     6541 cggattccag cagcagcgtt gccggacgat ccccacacaa ctggtgaaaa agcgcggtgg
     6601 gattgtcgcg ataagcgcct tcgcaggtta gcagttcgag agtcggtttt tgtgtttgca
     6661 ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat taggcgggct gggtatctga
     6721 ttgctttacg catggtgaat acactgcccg tttgaggaag tgcgccacca acctttcagt
     6781 acgaaaattg ctttcattgt cgataccctt tttacgtgaa cttgtgtact agttaactag
     6841 ttcgatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt tatgatgtcg
     6901 gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt atgcagtgat
     6961 ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt
     7021 gcaccgtgca gtcgagatgc gcgtcggcac cctggcgatc accgaccatg acaccacagc
     7081 tgcaatcgcg ccagcaagag aggaaatttc acgttcggga ctggcgttga atcttattcc
     7141 cggcgtggaa atttccacgg tctgggaaaa tcatgaaatt catattgtcg ggctgaatat
     7201 tgatattact catccgctaa tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca
     7261 gcgggcgcag ctgattgccg aaagacttga aaaagcgcaa atacctgggg cgctggaagg
     7321 cgcacaacga ctggcgcagg gcggcgcagt gacgcgcggt cattttgcgc gtttccttgt
     7381 tgagtgcggc aaagccagtt caatggcgga tgtctttaaa aagtatctgg cgcgcgggaa
     7441 aaccggatac gttccgccac agtggtgtac aatagaacaa gctattgatg tcattcatca
     7501 ttctggcggt aaggcggtgc tagctcatcc tgggcggtac aatcttagtg ctaaatggct
     7561 gaaaagattg gttgcgcatt ttgccgaaca ccacggtgac gcgatggaag tcgcgcagtg
     7621 ccagcaatcg cccaatgaac gtacccagct ggcggctctt gcgcgtcagc atcatttatg
     7681 ggcatcacaa ggatctgatt ttcatcagcc atgcccgtgg atcgaactgg gtcgtaaact
     7741 ctggctgccc gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc agaacaccac
     7801 agagagggaa ttatgagcca gtttttttat attcatcctg ataacccaca gcaacgtctg
     7861 atcaaccagg cggtggagat cgtgcgtaaa ggcggggtga ttgtttatcc aactgattcc
     7921 ggctatgcgc tcggctgtaa aattgaagat aaaaacgcga tggagcgtat ttgtcgtatt
     7981 cgccagctgc cggacggtca caactttacc ctgatgtgtc gcgatctttc tgaactctcg
     8041 acctattcat ttgttgataa cgttgcgttt cgtttaatga aaaacaacac gccgggcaac
     8101 tataccttca tcttgaaggg gacgaaggaa gtgccacgcc gtctgttgca ggaaaaacgc
     8161 aaaaccatcg gtatgcgtgt gccttctaac ccgattgcgc aggcgttgct tgaagcactg
     8221 ggcgaaccga tgctttccac ttcgctaatg ctgccaggca gcgaatttac cgaatccgat
     8281 cccgaagaaa ttaaagaccg tctggaaaaa caggtggatt tgattatcca cggcggctat
     8341 ctcggacaga aaccgacaac ggttattgat cttaccgatg atacgccggt cgtggtgcgt
     8401 gaaggcgtag gtgatgtgaa gcctttctta taagtgcgta actctgtata ctacgcggcc
     8461 ttaaaatggc gctatacggc gccattgatt ctcctgttcg acgcctgtga aggcgacacc
     8521 aaaaggaagc tctatgagcg aaaaattaca gaaagtgctg gcccgcgctg gccacggctc
     8581 ccgccgtgaa atcgaaacta ttattgaagc cggtcgtgtg agtgttgatg gcaaaattgc
     8641 taaactcggc gatcgcgttg aagttacccc tgggctgaaa attcgtatcg atggtcatct
     8701 gatttcggta cgtgagtccg ctgaacaaat ttgtcgcgtt ctggcctatt acaaaccgga
     8761 aggtgagttg tgtacccgta acgatccgga agggcgtccg acggtgtttg accgtctgcc
     8821 aaagctgcgt ggcgcacgct ggattgccgt aggtcgtctg gacgttaaca cctgtggtct
     8881 gctgctgttc accaccgatg gtgaactggc aaaccgttta atgcatccaa gccgtgaagt
     8941 tgaacgtgaa tacgccgtgc gtgtatttgg tcaggttgac gacgcgaaac tgcgtgattt
     9001 gagtcgtggc gtgcaactgg aagatggtcc ggcagctttt aaaaccatca agttcagcgg
     9061 cggcgaaggg atcaaccagt ggtacaacgt gactctgacc gaaggccgta accgtgaagt
     9121 tcgtcgtctg tgggaagcgg ttggtgtgca ggtgagccgc ctgatccgtg ttcgttacgg
     9181 tgatatccca ctgccaaaag gtctgccacg cggtggctgg acggagctgg acctcgccca
     9241 gactaactat ctgcgcgaac tggtggagct accgccagaa accagctcta aagtcgctgt
     9301 agaaaaagac cgtcgtcgca tgaaggcgaa tcagattcgt cgtgcggtga aacgtcacag
     9361 tcaggtgagc ggcggtcgtc gttctggcgg acgtaataac ggttaatcaa agtatgccgg
     9421 acgtcatatc cggcattttt acagattagt aatcaatccc tatctgcgct tttacaccgg
     9481 catcaaacgc atgtttgacg gggcgtaatt cacttaccgt atctgccagt tcaagaatat
     9541 cccgatgaca accacgaccc gtgataatca ccgtctgttg atgtggacgt tcatttaacg
     9601 cctgcaccac ttcttccagt ggcaaatagt catacgccac catatacgtc agttcatcaa
     9661 gcaaaaccat atccagtgag gaatcagcaa gcatccgctt tgcatgttgc cagacttcgc
     9721 ggcaggcggc ggtatcagac tcgcggtttt gtgtatccca ggtaaagccc gttgccatca
     9781 cctgaaactc aacgccatgt ggctccagca gattgcgttc gccattaggc caggtgcctt
     9841 taataaactg cacgacgcct actttttttc cgtgaccaac tgcgcgtgtt gccgtaccaa
     9901 atgccgcggt ggttttgcct tttccattgc cggtaaagac gatgataata ccgcgttcat
     9961 cctgggcctg ggccacacga gcatctactt tttctttcac tcgctgctga cgctgttggt
    10021 agcgttcatc actcattggg aaattcctgg tttacggccc ggttgggcgt caaaggtcat
    10081 gccggtttta cggcggctgt catcgcccat cagccagagg tagagcggca tgatatcagc
    10141 gggtgtttta agtttctgtg gatcttcggt cgggaaggcg ctggcacgca ttgcggtgcg
    10201 cgtaccgcct gggttaatgc agttgacgcg caggcgctgc tgatattcat cggccagtac
    10261 ctgcatcatc ccttcggtgg caaatttcga cgctgcatat gcgccccagt tggctcgtcc
    10321 ctgacgtcca acgcttgatg aagtaaagac cagtgaaccg gcgtccgatt tgagtaataa
    10381 aggaagcagt gcctgggtga gcataaaggt ggcattaacg ttgacctgta tgacgtcctg
    10441 ccagacctgt ggattttgtt cgctcattgg gcaaacatcg ccgagcaatc cggcattatg
    10501 caaaacacca tccagacgcg gataattaac ggtaatgcgc tgtgccagtt gttggcaatc
    10561 ttcggacgtg caggtcagca aatcgagaat aaaccactgt ggctgacgtc cagtttcttc
    10621 gtttatgtgg ctggctacct gacgtaattt ttcttcatta cggcccaaca gaatcactgt
    10681 cgcaccatag cgtgcatacg tcatcgcggc ttcacggcca atcccatcgc tggctcccgt
    10741 caccaggata atgcgatcat tgagtaaatc ttgttttggc tggtaatgca tggctactcc
    10801 tcaacgacgt tgtctgtcgc gctgccagtg tactttatga ctttggggct ttatgcctga
    10861 aacagaaggg catttcaatc agcctggggc gataacaccg caaaatggat actttgtcat
    10921 actttcgctg caataacatc tctgcgagac ggcttaacat gcctgttgta aactgtgagc
    10981 caaagcgttg tttaaccaag gtggggactc gtggaattgt tgtctgaata tggtttgttt
    11041 ttggcgaaaa tcgttaccgt tgtgctagcg attgcggcga ttgccgccat tattgtcaat
    11101 gttgctcaac gtaataaacg ccagcgtggc gagttacggg tcaacaatct cagcgaacag
    11161 tataaggaga tgaaagaaga actggccgcg gcgctgatgg actcacatca gcaaaaacag
    11221 tggcacaaag cgcagaagaa aaagcacaag caagaagcga aagcagcaaa agcgaaagcc
    11281 aaactgggcg aggtggcaac tgacagtaaa cctcgcgtct gggtgctgga ttttaaaggc
    11341 agcatggacg cccatgaagt gaactcgcta cgtgaagaga taacggcggt actcgcagca
    11401 ttcaaaccgc aggatcaggt tgtgctccgt ctggaaagcc ctggtggcat ggtgcatggt
    11461 tacgggttgg cggcttcgca gctgcagcgt ctgcgtgata aaaacattcc tttaactgtt
    11521 acggtagaca aagtcgctgc cagcggcggt tacatgatgg cctgtgtggc ggacaaaatt
    11581 gtttccgcac cgtttgctat tgtgggttcc attggggtgg tggcgcaaat gcccaacttt
    11641 aaccgcttcc tgaaaagcaa agatattgat atcgaactgc acactgccgg gcagtataag
    11701 cgtactctga cgttgctggg tgaaaatacc gaagaagggc gggagaaatt ccgcgaagag
    11761 ttgaacgaaa cgcatcagtt gtttaaagat tttgtgaagc gtatgcgtcc gtctctggat
    11821 attgaacagg tggcaacggg tgaacactgg tacggacaac aggcggtaga gaaaggcctg
    11881 gttgatgaaa tcaacaccag tgatgaagtt attcttagcc tgatggaagg ccgtgaagtg
    11941 gttaatgtac gctatatgca gcgtaaacga ctcattgacc gattcaccgg cagcgcggca
    12001 gagagcgccg atcgattgtt gctacgctgg tggcagcggg gtcaaaagcc attgatgtaa
    12061 aagacaaacg cgaggctaag acctcgcgtt ttgctttaat caaccagatg atatttttct
    12121 gaaagcacat gggccaggtg tttgaacata ttaaacaccg cggtgctttt ggctgttggc
    12181 aatccttgtt catctaaaaa gtagtcgccg gtaaatacca gcacgccatt acgctgcgtg
    12241 acgccggtgg cttcaatccc tgcgagcgta tcctcatgct cacgaatgat tttgttggcc
    12301 tctttcaaca gcgtttcgcg gtcgatgggt tgtgtctctt tgttcattat ttactcctta
    12361 aacaaggaca ttagtctacg ccaggcatgg cttgcagaca aatataccac gctggtggca
    12421 agagcgcctt actggcaact ttggattttg catgctaata aagttgcgta tcggatttta
    12481 tcaggtacag tgtgacgctt tcgtcaatct ggcaatagat ttgcttgaca ttcgaccaaa
    12541 attccgtcgt gctatagcgc ctgtaggcca agacctgtta actcagtcac ctgaattttc
    12601 gtgaacagag tcacgacaag gggttgatat ccgcagagaa cgagtccata tcggtaactc
    12661 gttgccagtg gaaggtttat caacgtgcga cgcattcctg gaagaatcaa attaggtaag
    12721 gtgaatatgg gtaaagctct tgtcatcgtt gagtccccgg caaaagccaa aacgatcaac
    12781 aagtatctgg gtagtgacta cgtggtgaaa tccagcgtcg gtcacatccg cgatttgccg
    12841 accagtggct cagctgccaa aaagagtgcc gactctacct ccaccaagac ggctaaaaag
    12901 cctaaaaagg atgaacgtgg cgctctcgtc aaccgtatgg gggttgaccc gtggcacaat
    12961 tgggaggcgc actatgaagt gttgcctggt aaagagaagg tcgtctctga actgaaacaa
    13021 ctggctgaaa aagccgacca catctatctc gcaaccgacc ttgaccgcga aggggaagcc
    13081 attgcatggc acctgcggga agtgattggg ggtgatgatg cgcgctatag ccgagtggtg
    13141 tttaacgaaa ttactaaaaa cgcgatccgc caggcattta ataaaccggg tgagctgaat
    13201 attgatcgtg ttaatgccca gcaggcgcgt cgctttatgg accgcgtggt ggggtatatg
    13261 gtttcgccgc tgctatggaa aaagatcgct cgtggtctgt ctgccggtcg tgtgcagtcg
    13321 gtggcggttc gcctggtggt cgagcgtgag cgtgaaatta aagcgttcgt gccggaagag
    13381 ttctgggaag tcgatgccag cacgaccacg ccatctggtg aagcgttggc gttacaggtg
    13441 actcatcaga acgacaaacc gttccgtccg gtcaacaaag aacaaactca ggctgcggta
    13501 agtctgctgg aaaaagcgcg ctacagcgtg ctggaacgtg aagacaaacc gacaaccagt
    13561 aaacctggcg ctccttttat tacctctacg ctgcaacaag ctgccagcac ccgtcttgga
    13621 tttggcgtga aaaaaaccat gatgatggcg cagcgtttgt atgaagcagg ctatatcact
    13681 tacatgcgta ccgactccac taacctgagt caggacgcgg taaatatggt tcgcggttat
    13741 atcagcgata attttggtaa gaaatatctg ccggaaagcc cgaatcagta cgccagcaaa
    13801 gaaaactcac aggaagcgca cgaagcgatt cgtccttctg acgtcaatgt gatggcggaa
    13861 tcgctgaagg atatggaagc agatgcgcag aaactgtacc agttaatctg gcgtcagttc
    13921 gtagcctgcc agatgacccc agcgaaatat gactccacga cgctgaccgt tggtgcgggc
    13981 gatttccgcc tgaaagcacg cggtcgtatt ttgcgttttg atggctggac aaaagtgatg
    14041 cctgcgttgc gtaaaggcga tgaagatcgc atcttaccag cagttaataa aggcgatgct
    14101 ctgacgctcg ttgaacttac accagcccag cactttacca agccgccagc ccgtttcagt
    14161 gaagcatcgc tggttaaaga gctggaaaaa cgtggtatcg gtcgtccgtc tacctatgcg
    14221 tcgatcattt cgaccattca ggatcgtggc tacgtgcgag tagaaaatcg tcgtttctat
    14281 gcggaaaaaa tgggcgaaat cgtcaccgat cgccttgaag aaaatttccg cgagttaatg
    14341 aactacgact ttaccgcgca gatggaaaac agcctcgacc aggtggcaaa tcacgaagca
    14401 gagtggaaag ctgtactgga tcacttcttc tcggatttca cccagcagtt agataaagct
    14461 gaaaaagatc cggaagaggg tggtatgcgc ccgaaccaga tggttctgac cagcattgac
    14521 tgcccgactt gtggtcgcaa aatggggatt cgcacagcga gcaccggggt attccttggc
    14581 tgttctggct atgcgctgcc gccgaaagag cgttgcaaaa ccaccattaa cctggtgccg
    14641 gaaaacgaag tgctgaacgt gctggaaggc gaagatgctg aaaccaacgc gctgcgcgca
    14701 aaacgtcgtt gcccgaaatg cggcacggcg atggacagct atctcatcga tccgaaacgt
    14761 aagttgcatg tctgtggtaa taacccaacc tgcgacggtt acgagatcga agagggcgaa
    14821 ttccgcatta aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg ctctgaaatg
    14881 cacctgaaaa tggggcgttt cggtaaatac atggcctgca ccaacgaaga gtgtaaaaac
    14941 acacgtaaga ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc ggtgccatta
    15001 cctgagctgc cgtgcgaaaa atcagatgct tatttcgtgc tgcgtgacgg tgctgccggt
    15061 gtgttcctgg ctgccaacac tttcccgaaa tcgcgtgaaa cgcgtgcgcc actggtggaa
    15121 gagctttatc gcttccgcga ccgtctgccg gaaaaactgc gttatctggc cgatgcgcca
    15181 cagcaggatc cggaaggtaa taagaccatg gttcgcttta gccgtaaaac caaacagcaa
    15241 tatgtctctt cggaaaaaga cggaaaggcg actggctggt cagcatttta tgttgatggc
    15301 aaatgggttg aaggaaaaaa ataaccttta attctgtcag gtttttataa acaaagggtc
    15361 gcgaaagcgg ccctttttta ttgcatatta ttttttcttc acacctatac ac
//
LOCUS       DAJSKJ010000090        15326 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000090 DAJSKJ010000000
VERSION     DAJSKJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.090"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            208..1866
                     /locus_tag="ON345_002386"
     CDS             208..1866
                     /locus_tag="ON345_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR3072437.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEECWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQACHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HCSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1863..2819)
                     /locus_tag="ON345_002387"
     CDS             complement(1863..2819)
                     /locus_tag="ON345_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR3072438.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2970..4085
                     /gene="macA"
                     /locus_tag="ON345_002388"
     CDS             2970..4085
                     /gene="macA"
                     /locus_tag="ON345_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR3072439.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4082..6028
                     /gene="macB"
                     /locus_tag="ON345_002389"
     CDS             4082..6028
                     /gene="macB"
                     /locus_tag="ON345_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR3072440.1"
                     /translation="MTPLLELKDIRRSYPAGDKQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6101..6325)
                     /gene="cspD"
                     /locus_tag="ON345_002390"
     CDS             complement(6101..6325)
                     /gene="cspD"
                     /locus_tag="ON345_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR3072441.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6648..6968
                     /gene="clpS"
                     /locus_tag="ON345_002391"
     CDS             6648..6968
                     /gene="clpS"
                     /locus_tag="ON345_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR3072442.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6999..9275
                     /gene="clpA"
                     /locus_tag="ON345_002392"
     CDS             6999..9275
                     /gene="clpA"
                     /locus_tag="ON345_002392"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR3072443.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9620..9707)
                     /locus_tag="ON345_002393"
     tRNA            complement(9620..9707)
                     /locus_tag="ON345_002393"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9671..9673),aa:Ser,seq:gga)
     gene            9941..10879
                     /gene="ghrA"
                     /locus_tag="ON345_002394"
     CDS             9941..10879
                     /gene="ghrA"
                     /locus_tag="ON345_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR3072444.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     LLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            10934..11671
                     /gene="ycdX"
                     /locus_tag="ON345_002395"
     CDS             10934..11671
                     /gene="ycdX"
                     /locus_tag="ON345_002395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR3072445.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKSSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            11695..12249
                     /gene="ycdY"
                     /locus_tag="ON345_002396"
     CDS             11695..12249
                     /gene="ycdY"
                     /locus_tag="ON345_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR3072446.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            12351..12842
                     /locus_tag="ON345_002397"
     CDS             12351..12842
                     /locus_tag="ON345_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR3072447.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(12906..13739)
                     /gene="csgG"
                     /locus_tag="ON345_002398"
     CDS             complement(12906..13739)
                     /gene="csgG"
                     /locus_tag="ON345_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR3072448.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAHSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(13766..14182)
                     /gene="csgF"
                     /locus_tag="ON345_002399"
     CDS             complement(13766..14182)
                     /gene="csgF"
                     /locus_tag="ON345_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR3072449.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(14207..14596)
                     /gene="csgE"
                     /locus_tag="ON345_002400"
     CDS             complement(14207..14596)
                     /gene="csgE"
                     /locus_tag="ON345_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR3072450.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(14601..15251)
                     /gene="csgD"
                     /locus_tag="ON345_002401"
     CDS             complement(14601..15251)
                     /gene="csgD"
                     /locus_tag="ON345_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR3072451.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         3768 a         3575 c         4086 g         3897 t
ORIGIN      
        1 ctttaggcgt aataaaaaat aatttatcat gctaattatt tgattttgtt gtttttgcag
       61 acttatcagc aagagggagt ataacgcgat tattcgctca tttttcagac atttgccatg
      121 cttaaatgtg atgtcatcac gtattagcaa ggcctttccc gttatactgc cagcgtaaag
      181 gataagtcac atatttctgg aggggatatg attcttgagc gcgttgaaat tgtgggtttt
      241 cgcggtatca accgtttgtc gttgatgctc gaacaaaaca acgtcctgat tggggagaac
      301 gcgtggggta aatccagctt gctggacgcc ttaaccctgc tgctatcgcc agaatcagat
      361 ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa
      421 catcatctgc atattatttt gaccttccgc gaatcgctgc caggccgaca tcgggttcgc
      481 cgttatcggc cgctggaaga gtgctggacg ccatgcaccg atggctatca ccgtattttt
      541 tatcgtctgg aaggggagag tgcggaagac ggcagcgtga tgacactgcg cagttttctc
      601 gataaagacg gacatccgat tgatgttgag gatattaacg atcaggcatg ccatctggtg
      661 cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc
      721 acggtgccaa atgtccccaa tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt
      781 gagttgtcct cacatccgca aaatctctca gatggacaga ttcgtcaggg actttccgca
      841 atggtgcagt tgcttgagca ttatttctct gagcaggggg ctggacaggc gcgatatcgt
      901 ttaatgcggc ggcgggccag caatgagcaa cgaagctggc gctatctgga tatcatcaac
      961 cggatgattg accgacctgg tgggcgctcg tatcgggtta ttttgctcgg cctgtttgct
     1021 actttgttgc aggcaaaagg cacattgcga ctggataaag acgcccgtcc attgttgctg
     1081 atcgaagatc cagaaacccg tttacacccc attatgcttt cagttgcctg gcatctgttg
     1141 aatcttctgc cattgcagcg cattgccacc accaactcgg gtgagttgct ttcgttaacg
     1201 ccggtagagc atgtttgccg actggtacgt gagtcctcgc gcgttgccgc ctggcgtctg
     1261 gggccgagtg gcttgagtac cgaagatagc cgacgcatat cctttcacat tcgttttaac
     1321 cgtccgtcat cgctgtttgc acgctgctgg ttgctggtgg aaggggaaac ggaaacctgg
     1381 gttatcaatg aactggcgcg tcagtgcgga catcattttg atgccgaagg gatcaaggtc
     1441 attgagtttg cccagtccgg gctaaagcca ctggttaaat ttgcccgccg aatggggatt
     1501 gaatggcatg tactggtcga tggcgatgaa gcagggaaga aatatgccgc tacggtacgc
     1561 agcctgttga ataacgatcg ggaagccgaa cgagaacatt taacggcgtt accggcgctg
     1621 gatatggaac attttatgta tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa
     1681 atcccggaaa atgtaccgat gaatctacgc aaaattatct cgaaagcgat ccattgctct
     1741 tccaaacccg atcttgccat tgaagtggca atggaggcag gacgtcgtgg tgtggactcc
     1801 gtaccgacgc tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg
     1861 gattaaccgc gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc
     1921 cggcggtatt cagcccgttt tttactggcg atttcggcaa tctcttttcg tgctatctgt
     1981 gctggaaggc ggtaatggcg ttcagcatca catacgccgc caaccgattc ccagaaagcg
     2041 ttgtaatcag cgtggatctt gccttcttta tcgcgataac gcaggctgcg gtaaatatgc
     2101 gtttcattgc tgacggcaat aatctgctct acctgcaaac gttgggcaaa cagacaggcc
     2161 gcttccatca cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc attctggatt
     2221 tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc caataaacat cgttcttttc
     2281 ccctgatatt cacacagggt aaacgtgatc tctgccagag gaataccttc gctgttgcga
     2341 aacaggattg tgctgtcacc ttctttatcc attgagatca tcatggtcag ctcaagcgtg
     2401 aactgctcgc cgtttttgcc ttccagcttc gccagttgca gcccgggggt attcaaatat
     2461 aagctgaatt cttccgccga catacatccg cggagtaacg cataatggta acgtaacgcc
     2521 tccagcaatt gcttacggct aagattcgcc gcaaggtaag ggcgatgcag acgtacaggc
     2581 agtcgcggtt ggcgcgttaa caacacattg agattaggcc agtgggaaag ttcgttcatc
     2641 cactcaacgc ttaaacgcgg cataatcaac gagcgcagca aaaatttctg gcgaaaacta
     2701 cggcgatgcc agaatttacc cggccgacac tgtccacgtg ccagactaag aaaaagtgac
     2761 aggctgctga gagattcaga tggcgtaaag gtccgttcag ttagctgcga catattcatg
     2821 aaatcaatgg ttatacatga cgtcgatttc accattgcgt atcttaacca aacatcaata
     2881 gtgtgattac taacgtaaat tttatggttt tgttgatatt tcgttgaagt taatgacccg
     2941 gattggcata tggagtattc agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt
     3001 acgttattgc gctggtgata gtcatcgccg gactgattac gttgtggaga attcttaacg
     3061 cacccgtgcc gacttatcag acgctgattg tgcgccccgg tgatttacag caaagcgtgc
     3121 tggcgaccgg aaagctggac gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc
     3181 agttgaaaac tctgtcggtg gcgattggcg ataaagtaaa aaaagaccag cttttagggg
     3241 ttattgatcc tgaacaggct gaaaaccaga ttaaggaggt cgaagcaacg ctgatggagc
     3301 tacgtgcgca gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg acgtattccc
     3361 gtcagcaacg tctggcacaa acgcaggctg tttcacagca ggatctcgac accgccgcga
     3421 cggagatggc tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc
     3481 aggcttctct cgatacggct aaaaccaatc tcgattacac tcgcatcgtt gccccgatgg
     3541 ccggggaagt cacgcaaatc accactctgc aaggccagac ggtgattgcc gcacaacaag
     3601 caccgaacat tctgacgctg gcagatatga gcaccatgct ggtaaaagcg caggtttctg
     3661 aagcggatgt aatccacctg aagccggggc aaaaagcctg gtttacggta cttggcgatc
     3721 cactgacgcg ctacgagggg caaatcaagg atgtactacc gacgccggaa aaggttaacg
     3781 acgctatttt ctattacgcc cgttttgaag tccccaaccc caatggtttg ctgcggctgg
     3841 atatgactgc gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg acgatccctc
     3901 tgtcggcgtt aggcgatccg gttggcgata atcgttataa agtcaaattg ttgcgtaatg
     3961 gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat gttgagattg
     4021 tcaaagggct ggaagcgggc gatgaagtgg tgattggtga ggccaaacca ggagctgcac
     4081 aatgacgcct ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg gtgataagca
     4141 ggttgaggtg ctgaagggca tcaccctcga tatttatgcc ggtgagatgg tagcgattgt
     4201 tggcgcttca ggttccggta aatcgaccct gatgaatatt ctcggctgtc tggataaggc
     4261 caccagcggc acctatcgcg tcgccggtca ggatgttgcc acactggacg ccgatgcgct
     4321 ggcgcaactg cgccgcgagc atttcggctt tattttccag cgttaccatt tactttcgca
     4381 tttaaccgcc gagcagaacg ttgaagtacc cgccgtctat gccggtcttg agcggaaaca
     4441 gcggctgctt cgcgcccagg agttactgca acgactgggg ctggaagacc gtacagagta
     4501 ttatccggca cagctttcgg gtggtcagca acagcgcgtc agcatcgcgc gggcattgat
     4561 gaacggcggt caggtaattc ttgccgatga accaaccggc gcactggaca gccattctgg
     4621 cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt
     4681 cacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga
     4741 aattgtgcgc aatcctcccg ccattgaaaa agtgaatgtt gctggcggga cggagcctgt
     4801 tgtcaacacg gtgtctggct ggcggcagtt tgtcagcggt tttaacgagg cgctgacgat
     4861 ggcatggcgg gcgctggcag cgaataaaat gcgtacttta ctgaccatgc tggggattat
     4921 tatcggtatt gcgtcggtgg tttccattgt cgtggtgggt gacgccgcca aacaaatggt
     4981 gctggcggat attcgttcta ttggtacgaa tactattgat gtctatcccg ggaaagattt
     5041 tggcgatgac gatccgcaat atcagcaggc gctgaagtac gacgacttaa tcgccatcca
     5101 aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg caaaacctgc gcctgcgtta
     5161 taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta
     5221 tggcatgacc ttcagtgaag gaaacacctt taatcaggag cagctgaacg gtcgtgcgca
     5281 ggtcgtggtt ctcgacagta atactcgccg ccagcttttc ccccataaag cagatgtggt
     5341 tggcgaggtg attctggtcg gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa
     5401 acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc
     5461 cgggcgagtt atgggccagt cgtggcttaa ctccattact gtcagggtga aagaaggatt
     5521 tgacagcgcc gaggcggaac agcaactcac gcgtttactt tcactgcgcc acggtaagaa
     5581 ggatttcttt acctggaaca tggacggcgt cttgaaaact gttgaaaaga ccacacgtac
     5641 tttacaactg tttctgacgc tggtggcggt gatttcgctg gtggtgggtg gtattggtgt
     5701 aatgaatatt atgctggtgt cagtgaccga gcggacgcgg gaaattggca ttcgcatggc
     5761 tgtaggtgcg cgagcaagcg atgttttgca acagttcctg atcgaagccg tactggtttg
     5821 cttggtcggt ggcgcgttgg gaataactct gtcactgtta attgctttca ccttgcagct
     5881 tttcttaccc ggctgggaga ttggtttttc accgttggcg ctgctgctgg cgtttctctg
     5941 ctcgacggtc accgggattt tatttggctg gttacccgca cgaaatgcgg cacgactgga
     6001 tccagtagat gctctggcac gagagtaatt tttgagataa aaatgccagc cgatcggact
     6061 ggcattttgc ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta
     6121 cttcgacggg cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact
     6181 gaacggattg tccagctttt agcgttctgt aaccatccat ctgaatggtg gaataatgag
     6241 cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga
     6301 accacttaac agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag
     6361 atggaataaa ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca
     6421 atttagagaa ttagggcgag ccgtcaagcg tttgacaggg gccagggggc aggtatgaat
     6481 caaaaatttg aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac
     6541 gatagatgat agttatctat catgtggagt agattggtca ggcaaataag ctcttgtcag
     6601 cggcagggcg ttctgccgat aaccgtaacc gaagatgata actgacaatg ggtaaaacga
     6661 acgactggct ggactttgat caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc
     6721 catctatgta taaagtgata ttagtcaatg atgattacac tccgatggag tttgttattg
     6781 acgtgttaca aaaattcttt tcttatgatg tagaacgtgc aacgcaattg atgctcgctg
     6841 ttcactacca ggggaaggcc atttgcggag tctttaccgc cgaggttgca gaaaccaaag
     6901 tggcgatggt gaacaagtac gcgagggaga atgagcatcc attgctgtgt acgctagaaa
     6961 aagcctgaat gcaggcataa aaattggggg aggtgcctat gctcaatcaa gaactggaac
     7021 tcagtttaaa tatggctttc gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg
     7081 agcacttgtt actggcgctg ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt
     7141 ctgtggattt ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa accacacccg
     7201 ttctgcctgc cagtgaagag gagcgcgaca cacagccgac gctgagtttt cagcgtgtac
     7261 tgcaacgtgc ggtcttccat gtccagtcct ccggtcgcaa tgaggtaacc ggtgcaaacg
     7321 ttctggtcgc tatctttagc gaacaggagt cgcaggcggc atatctgttg cgtaaacatg
     7381 aagtcagccg tctcgatgtg gtgaacttta tctctcatgg cacgcgtaaa gacgagccga
     7441 cacagtcttc tgatcctggc agccagccaa acagcgaaga acaagctggt ggggaggaac
     7501 gtatggagaa tttcacgacg aacctgaatc agcttgcgcg cgtgggcgga atcgacccac
     7561 tgattggtcg tgagaaggag ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa
     7621 acaacccgct gctggtgggg gaatctggtg tcggtaaaac cgcgattgcg gaaggtcttg
     7681 cctggcgaat tgttcagggc gatgtgccgg aagtgatggc tgactgtacg atttactctc
     7741 tcgatatcgg ttctctgtta gcgggcacta aatatcgcgg cgactttgaa aaacgtttta
     7801 aagcgttgct caagcagctg gagcaggaca ctaacagcat cctgtttatt gatgagatcc
     7861 acaccattat cggtgcgggt gcagcgtctg gtggccaggt cgatgcggct aacctgatca
     7921 aaccgttgct ctccagcggt aaaattcgcg taattggttc gacaacctat caggagttca
     7981 gcaacatttt cgagaaagac cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg
     8041 aaccgtcgat cgaagaaact gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc
     8101 accacgacgt gcgttatacc gcaaaagcgg tgcgtgcagc ggtagagctg gcggtgaaat
     8161 acattaacga tcgtcatctg ccggataaag ccattgacgt tatcgacgaa gcgggcgctc
     8221 gcgcacgcct gatgccggta agcaaacgca agaaaaccgt taatgtggcg gatattgagt
     8281 ccgtggtggc ccgtattgcg cgcattccag agaagagtgt ttctcagagt gaccgcgata
     8341 ccctgaaaaa cctcagcgat cgcctgaaaa tgctggtctt cggtcaggat aaagccattg
     8401 aggcgctgac tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg
     8461 ttggttcgtt cctgtttgcc ggccctaccg gggtcgggaa aacagaggtg acggtacagc
     8521 tttcgaaagc gttgggcatt gagctgctgc gctttgatat gtccgagtat atggaacgcc
     8581 ataccgtcag ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat cagggcggtt
     8641 tgctgactga tgcggtcatc aagcatccac atgcggttct gctgctggac gaaatcgaga
     8701 aagcgcaccc tgacgtgttc aatattctgt tgcaggtgat ggacaacggt acgctgaccg
     8761 ataacaacgg acgcaaagcg gacttccgta acgtggtgct ggtgatgacc accaacgccg
     8821 gggtacgtga aactgagcgt aaatccattg gtcttatcca ccaggataac agcaccgatg
     8881 cgatggaaga gatcaagaag atctttacac cggaattccg taaccgtctc gacaacatta
     8941 tctggtttga tcatctgtca accgacgtga tccatcaggt ggtggataaa ttcatcgtcg
     9001 agttgcaggt tcagctggat cagaaaggtg tttctctgga agtgagccag gaagcgcgta
     9061 actggctggc cgagaaaggt tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca
     9121 tccaggacaa cctgaaaaaa ccgctcgcca acgaactgct gtttggttcg ctggtggacg
     9181 gcggtcaggt caccgtcgcg ctggataaag agaaaaatga gctgacttac ggattccaga
     9241 gtgcacaaaa gcacaaggcg gaagcagcgc attaatctga ttgttaggta ggttggtcaa
     9301 gtccgtaatc tcgaaagagg ttacggactt tttgtttatg gggtggagga ggttcagacc
     9361 ctttttttaa tgatgatggt aagttgttga taattagtgc tgcgggaagg taaggataaa
     9421 aaagggtgct gcaggagaat gggttggttt tgctttatta acaacgggct aaacgtgtag
     9481 tatttgagtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9541 ccaccatgtt cactgtcgta cagacagata aaatgcgaaa aaaaaagctc gcactttcgt
     9601 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9661 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgtcgttcc
     9721 tgtcttgctg gaacgggcgc taatttaggg aaatcacgac ctgaggtcaa caaacttttt
     9781 gaaaaaatcg cgcgtttatt caaacttcaa tcaatgtgtg gttttaataa gcgaaatctg
     9841 ctttttttgc caccgaccac ggatttgtta tgctggtggc ctttgtagat cataacgata
     9901 agtgcgaata aatttcgcac aacgcttttc gggagtcagt atggatatca tcttttatca
     9961 cccaacgttc gatacccaat ggtggattga ggcactgcgc aaagctattc ctcaggcaag
    10021 agtcagagca tggaaaagcg gagataatga ctctgctgat tatgctttag tctggcatcc
    10081 tcctgttgaa atgctggcag ggcgcgatct taaagcggtg ttcgcactcg gggccggtgt
    10141 tgattctatt ttgagcaagc tacaggcaca ccctgaaatg ctgaaccctt ctgttccact
    10201 ttttcgcctg gaagataccg gtatgggcga gcaaatgcag gaatatgctg tcagtcaggt
    10261 gctgcattgg tttcgacgtt ttgacgatta tcgcatccag caaaatagtt cgcattggca
    10321 accgctgcct gaatatcatc gggaagattt taccatcggc attttgggcg caggcgtact
    10381 gggcagtaaa gttgctcaga gtctgcaaac ctggcgcttt ctgctgcgtt gctggagtcg
    10441 aacccgtaaa tcgtggcctg gcgtgcaaag ctttgccgga cgggaagaac tgtctgcatt
    10501 tctgagccaa tgtcgggtat tgattaattt gttaccgaat acccctgaaa ccgtcggcat
    10561 tattaatcaa caattactcg aaaaattacc ggatggcgcg tatctcctca acctggcgcg
    10621 tggtgttcat gttgtggaag atgacctgct cgcggcgctg gatagcggca aagttaaagg
    10681 cgcaatgttg gatgttttta atcgtgaacc cttaccgcct gaaagtccgc tctggcaaca
    10741 tccacgcgtg acgataacac cacatgtcgc cgcgattacc cgtcccgctg aagctgtgga
    10801 gtacatttct cgcaccattg cccagctcga aaaaggggag agggtctgcg ggcaagtcga
    10861 ccgcgcacgc ggctactaat aaagcatcag gattcctgct atccttggcg ggaattgaat
    10921 acaggagaga gttatgtatc ccgtcgacct tcatatgcat accgttgcca gcacacatgc
    10981 atatagcaca ttgagtgatt acattgccca ggccaaacaa aagggcatta aactttttgc
    11041 gatcaccgat catggcccgg atatggaaga tgctccgcat cactggcact tcattaacat
    11101 gcgtatctgg ccgcgagtgg ttgatggggt agggatcctg cgcggcatcg aagctaacat
    11161 taaaaatgtt gatggtgaaa ttgattgcag cggtaaaatg tttgactcgc tggatctgat
    11221 tattgccggt tttcatgagc cggtttttgc accacatgac aaagcgacca atacacaagc
    11281 gatgatcgcc actatcgcca gcggcaatgt gcatataata agccatcccg gaaatcccaa
    11341 atatgaaata gatgtgaaag ccgttgctga agcagccgcg aaacatcagg tggcgctgga
    11401 aatcaataat tcttcatttt tacactcacg taagagcagt gaagacaact gtcgtgcggt
    11461 agccgcagcg gtacgtgatg ctggaggttg ggtggcatta ggctcggatt ctcacaccgc
    11521 gtttaccatg ggggaatttg aagagtgtct taaaatcctc gacgcggtag attttccgcc
    11581 agagcgcatt ttgaatgttt ctccgcgccg cttactgaac ttccttgaat ctcgcggtat
    11641 ggcaccgatt gcggaatttg cagaccttta attactagta atggaaatat attaatgaac
    11701 gagttttcta tcctctgtcg tgtgctgggt tcgctctatt accgccaacc gcaagatcct
    11761 ttactggtgc cgctgtttac cctgattcgt gaggggaaac tggctgcgaa ctggccactg
    11821 gagcaggatg agttactgac gcgtttacag aaaagctgtg atatggcgca agtctctgcc
    11881 gattacaatg cgttgtttat cggcgatgaa tgtgccgtgc cgccatatcg tagcgcatgg
    11941 gttgaggacg caacggaagc ggaagtgcgc gcttttcttt ccgaacgagg gatgccactg
    12001 gcggatacgc cagccgatca catcggcaca ttgctgcttg cggcttcctg gctggaagat
    12061 cagtcaacgg aagatgagag cgaagcactg gaaacactgt tcagtgagta tctgttaccc
    12121 tggtgtggtg cgttcctcgg caaagtggag gcccatgcaa ccacgccttt ctggcgcacc
    12181 atggcaccgc taacccgtga tgccattagt gcaatgtggg acgagctgga agaagattct
    12241 gaagagtaaa tgtgatcatc gtcactcata actgcaacag ataatttgtt gttgcataaa
    12301 atgtgtgctc gatctcattc atggccgcgt tttctgctat catgcgcggc atgaacatac
    12361 ttctctctat tgcaatcaca acaggcattc tctccggtat ctggggatgg gtggctgttt
    12421 ccctgggctt actaagctgg gcgggcttcc tgggctgtac ggcctacttt gcttgcccgc
    12481 aaggtgggct gaaagggctg gcgatctccg ctgcaaccct gcttagtggc gtggtgtggg
    12541 cgatggtcat tatttacggt agtgcactgg caccacatct ggaaattctc ggttatgtca
    12601 taaccggtat tgtcgcgttt ctgatgtgta ttcaggccaa acagctgctg ctttcatttg
    12661 ttccgggaac gtttataggc gcatgcgcga cgtttgccgg gcagggtgac tggaaactgg
    12721 tgttaccttc gctggcgctt gggctgatat ttggttacgc aatgaaaaac agcggcctgt
    12781 ggctggcggc gcgtagtgca aagacggcgc accgtgagca ggaaatcaaa aataaagcgt
    12841 gaggggcact cacgctttcg cttaaacagt aaaatgccgg atgataattc cggctttttt
    12901 atctgtcagg attccggtgg aaccgacata tggcggtatt tcaccagaat gtcgttctgc
    12961 cgttctgctt tgttctgcaa atcccacaga ccacggtcaa taccatcatt aatcaggaaa
    13021 atgacccctg tttcgatagc cgacatcagg cacagcataa cgggttcgtt cgaggtgtaa
    13081 cccacttccc cttccagcaa gcgctggtag tcaataaagc ggaaaacccc ggcctgaact
    13141 tcataggaaa gtatcgtctt actggtgttc accgaggaga ggatctcacc ggtactcaca
    13201 ttgacgacac gcaggttcac ggcaatctga tcgagctggt attgcgtgtc ggcaccgatc
    13261 ccaaaatatc ttgccccaac accgcccgat ttaacgttgc tttcataacc gataatcgaa
    13321 ccttcaacca tgatatttgc cgccgttaaa gattgcagcg ggattcggtt attaatggca
    13381 actgtgccgt tttcttgtgc cgcacgaata atcttgcgct cgttaagcag gttttgtaaa
    13441 ccctgacgct ccagcggtat aaaccagcga gaatctttca atgccgtgac cagcattgcc
    13501 gtggcgcttt gcggaacagc agtggagaag ttacttgccg ggtagggttt aaattgcccg
    13561 gtttcgtcct gaatgttgta taccgaaaca aagatcttac ccgtcggcgc tggcagatgg
    13621 gtcaaatctt tgtagctatg agctcgaggc attaatgtcg gtctggcggc ttctttaggc
    13681 ggggcggtta agcatccgct cagtaacatg acggcaacca aaagaaataa gcgctgcatg
    13741 attattttcc ttatgaagct ggggcttaaa aatcggttga gttattttgt aaacccgaaa
    13801 cctggatggt cgaggtttgt ccggttttac gatctgtcac gttcaactgc aattgaccat
    13861 cgcggttagc aatatcaaca atataatcgt tggtcaccat gcggcccggt ttaccggtat
    13921 taatattcga cagtagccca cctaaaattt gtgactggat ggcctgagta aagttatcta
    13981 acgctgaggg tgtttcaata ccaaagtcat cgttatagct cggatcttta taagagtttt
    14041 gggcctgagc gctatttaat aaaaaagcgc cattatttgg gttaccacca aagtttggat
    14101 tacggaactg gaaagtcatg gttccagccc aacttaatgg cgaaataagc atgagtagaa
    14161 ctactgcatg tttgacacgc attgcagcct ccgaacaatt ttttatttag aattcatcat
    14221 gcgccaaatc gcccgtactt aataacgcct gatttatctg gcgacgattt agtgcttctt
    14281 cagtttgaat cagtgcaaag acgacagttt tctcgaagtc tcttttcaac ggaaataaaa
    14341 aagtctggaa aataacgtcc tgattgaccg ttatagtgat ccagcttccc catcgtgcac
    14401 tgggcctttc attaatcgtt aagttacccg tatagtcact ttcccattta tcactaaagg
    14461 ctcggtaaaa atcatggcca atagatgaaa cagtatggtc agttagcaat cccgggactt
    14521 ctacctcaac ggcgtgaaga ttccctgcgg cgaacagaaa ttctgccgcc acaatccagc
    14581 gtaaataacg tttcatggct ttatcgcctg aggttatcgt ttgcccagga aaccgcttgt
    14641 gtccggtttt ttacggctat cttcttgaaa agattataaa gatgcgtttt aaccgtattt
    14701 tcgctgatga acaacgaacg agcgatctcg ttattagacg cgccgatacg cagcttattc
    14761 aggatctctt tttcccgatg agtaaggagg gctgattccg tgctgttata acgatagtta
    14821 cctgaatgcg taatcaagta gctggcaagc ttttgcgtaa agtagcattc gccgcgcagg
    14881 acgccttgca acccattgac aacacgttct tgatcctcca tggcataaaa aacgccgttg
    14941 atatgaggcc agttttcaat gtcgcggtac gggtaatctt caggcgtatt tagcaacaat
    15001 attttgatat tgttgttttt cctgctcaaa gtatcctgcc aataatggat aagcttttta
    15061 tctgcttcca tcatatccag aagaataata gagcctgaag atatatcgtc cagagaacgt
    15121 tgaatattat gtaattttcc tgtaattgcc agcgattgtt taaggtgctg caagagagct
    15181 gtcgcttgca aagacggttt agtgatcaac aataatgtat gaccatgaat actatggact
    15241 tcattaaaca tgatgaaacc ccgctttttt tattgatcgc acacctgaca gctgcctcta
    15301 aaatagaagc accagaagta ctgaca
//
LOCUS       DAJSKJ010000091        15005 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000091 DAJSKJ010000000
VERSION     DAJSKJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.091"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..598)
                     /gene="fghA"
                     /locus_tag="ON345_002402"
     CDS             complement(<1..598)
                     /gene="fghA"
                     /locus_tag="ON345_002402"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR3072452.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIIVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYD"
     gene            complement(691..1800)
                     /gene="frmA"
                     /locus_tag="ON345_002403"
     CDS             complement(691..1800)
                     /gene="frmA"
                     /locus_tag="ON345_002403"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCR3072453.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAEAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(1835..2110)
                     /gene="frmR"
                     /locus_tag="ON345_002404"
     CDS             complement(1835..2110)
                     /gene="frmR"
                     /locus_tag="ON345_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCR3072454.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(2270..3316)
                     /gene="fbpC"
                     /locus_tag="ON345_002405"
     CDS             complement(2270..3316)
                     /gene="fbpC"
                     /locus_tag="ON345_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HCR3072455.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(3328..5406)
                     /locus_tag="ON345_002406"
     CDS             complement(3328..5406)
                     /locus_tag="ON345_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3072456.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREVTREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(5475..6506)
                     /locus_tag="ON345_002407"
     CDS             complement(5475..6506)
                     /locus_tag="ON345_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3072457.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(6503..7819)
                     /gene="uhpC"
                     /locus_tag="ON345_002408"
     CDS             complement(6503..7819)
                     /gene="uhpC"
                     /locus_tag="ON345_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR3072458.1"
                     /translation="METTMHARSAREIDQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(7892..9433)
                     /locus_tag="ON345_002409"
     CDS             complement(7892..9433)
                     /locus_tag="ON345_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR3072459.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPVLLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFGERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(9433..10062)
                     /locus_tag="ON345_002410"
     CDS             complement(9433..10062)
                     /locus_tag="ON345_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3072460.1"
                     /translation="MIRIVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            10365..11327
                     /gene="tauA"
                     /locus_tag="ON345_002411"
     CDS             10365..11327
                     /gene="tauA"
                     /locus_tag="ON345_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3072461.1"
                     /translation="MAISSRNTLLATLAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEDDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            11340..12107
                     /gene="tauB"
                     /locus_tag="ON345_002412"
     CDS             11340..12107
                     /gene="tauB"
                     /locus_tag="ON345_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR3072462.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESSELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            12104..12931
                     /gene="tauC"
                     /locus_tag="ON345_002413"
     CDS             12104..12931
                     /gene="tauC"
                     /locus_tag="ON345_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR3072463.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIHAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            12928..13779
                     /gene="tauD"
                     /locus_tag="ON345_002414"
     CDS             12928..13779
                     /gene="tauD"
                     /locus_tag="ON345_002414"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR3072464.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(13885..14859)
                     /gene="hemB"
                     /locus_tag="ON345_002415"
     CDS             complement(13885..14859)
                     /gene="hemB"
                     /locus_tag="ON345_002415"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR3072465.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         3622 a         4070 c         4018 g         3295 t
ORIGIN      
        1 ggtcgtaatc caaccaggcc tctttatttt caccaagata tgcagcaaag gcttgctgtc
       61 cccacggcac ttgcgatggg gagacaatgg gcgaaaacgc cgagacgctg acatattcat
      121 ctgggttacg taacgccagc accagcgcgc ccagcccgcc catagaatga ccagagatag
      181 actttttggc cgttgccgga aaatgttgca tcactaaatc cggcagctcg ttgcggatat
      241 agtcatacat tttgtaatgt tcattccacg gcgcttgcgt cgcgttcagg taaaacccgg
      301 caccttgccc gagatcgtaa cggtcggcat ctgcgacatg actgcctcgc ggactggtgt
      361 ccggcgcaac aataataatg ttgtgctcag ccgcgtaacg ctgcatcccc gatttagtaa
      421 tgaaattctg ctcattgcag gtcaggcccg aaagccagta cagcacaggt aatttttcac
      481 ctgcagcttt tggtgggaga tagacgccga cattcatttc acatttcagt gatgcggaat
      541 aatgccgata cacattttgc cagccgccaa agctggcatg tttttcaatg agttccatca
      601 gtcattatct cacggttaat cccccggacg gctgcccggg gagagtatta agaaccccgc
      661 ctgaactgga cggggtaaac cagcgggaaa tcagtaacga attacggttc gaatggattt
      721 gccttcatgc atcaggtcga aggcgtcatt aatttcatcc aggctcatgg tatgcgtgac
      781 aaacggttcc agatcgatat cacctttcat cgcatcttca accatacccg gtaactggga
      841 acgacctttc acgccgccaa acgcggaacc tttccatacg cgaccagtga ccaactggaa
      901 tggacgggtg gagatttcct gaccggaacc cgcgaccccg atgatcaccg actgacccca
      961 gccgcggtgc gcactttcca gcgccgcacg catcacgttg acgttaccga tgcattcaaa
     1021 ggtatggtcg ataccccatt tgttgatatc cagcaggaca tctttaatcg gtttgtcgta
     1081 gtcattcggg ttaatgcagt cggtggcacc gaagcgacgc gccagatcga atttcttcgg
     1141 gttggtatcg atagcgataa tccgtcccgc ttccgcctga cgcgcgccct gaaccactgc
     1201 cagaccaatc gcgccaagac caaacacagc aacagaatcc cctggctgga ctttagctgt
     1261 gttgtgtacc gcgccaatac cggtggtcac gccacagccc agcaggcaga cgtgttcgtg
     1321 gtttgcttct ggattaattt ttgccagaga aacttccgcg accacggtgt attcactgaa
     1381 tgttgagcat cccatgtagt gataaagcgg ctgcccgttg taagaaaaac gggtagtgcc
     1441 gtctggcatc agtcctttgc cctgggtttc gcgaaccgca acacagaggt tagttttgcc
     1501 ggaacgacag aactcacact cgccgcactc cgcggtgtaa agcgggatca catggtcgcc
     1561 tggtttgacg ctggttacgc cttcaccgac ttcaaccaca acgccagccc cttcgtgacc
     1621 gagaaccacc gggaatacac cttccgggtc atcgccggag agggtaaatg cgtcggtatg
     1681 gcaaacgccg gtatgggtga ctttaatcag cacttcacct tttttcggtg gtgcaacgtc
     1741 aatttcaacg atttccagcg gtttaccggg agcaaatgca acggcagcac gtgatttcat
     1801 ctctcgctct tcctcaatat ggtaatagat tcagctattt aagataggca cgaaccagtt
     1861 caatagtgtc gtcaacggat tggctgactt cgcggctgta gcagtcattt cggtcaaacg
     1921 tttcccggat atggctttca agcacttctg ccatcagccc attagccgcg ccccgaacgg
     1981 cagcgatctg ttggagtatg gcacggcatt cggcatcacc ctccagcgac cgttccagag
     2041 catcaatctg ccctcgaata cgacgaactc gagtaaggac ctttttcttc tcttccggag
     2101 tactgggcat ttcgcacctc atcatctgca tgcaatatac tatagggggg tatgctatat
     2161 gtcaatgcat acccccctat agtatataac actggagaat aattgttgtc cgaagcctaa
     2221 ccaggcgaac ggtccctcaa gacagatggg gaccgtttat gtgctatttt tatgccgcat
     2281 ccgctaaaac aaacatcccg taaggatgga tttcgaggta atagtgctca ccaatatcgg
     2341 gctgtaaacg ggtggcgttt acttgtaata aaatctcctg tccatgccac tctacggtca
     2401 cttcgtactg cggccccatg taggcgacat ggcgaataac acaacgctga ctctcttcgc
     2461 cgtgattgct taaggtgatg gcttccgggc gaacgccgac ggtgccgcta ccttgcgccg
     2521 ggaaatgcgc cgcgcgcggc aggcgataac cgtagatatc gacatactct tcgctaaagt
     2581 tcgccgggaa caggttggcg tcgcccataa aacttgccat aaatcgggag gctggctgac
     2641 gatagagatc ctgcggtgag ccaatctgca tgatgtgacc tttattcatc accagcacag
     2701 tgtcggacac cgcgaaagct tcactttgat cgtgagtgac gtagagcgac gtgatattaa
     2761 actgcttttg cagctcgcgg atcttatcgc gcatactgcg gcgcaggttg gcatcgaggt
     2821 tacttaacgg ctcatcaaac agcagcacct ttggcttgag gatcaacgcg cgggccagtg
     2881 ccacacgctg ttgctgacca ccggaaatct gatcgacata gcggtcctcg aagccttcca
     2941 gatccaccat tgccagcgcc tccttcaccc gctgtttcac ttcgctacgc gacacgccaa
     3001 gcatcttcag cccgtagcca acgttttcac ccagcgacat atgcgggaac agagcgtatg
     3061 actgaaacac catgcagata tcgcgctgtt gaatggaacg atgcgtgaca tcttcgccat
     3121 caataaaaat ttgcccttcg ctcggttttt ccagcccggc aaccagacgc aaaatggtgg
     3181 ttttgccaca gccggaagga ccgagcagcg tcaccatttg cccttgtgga atggtgagat
     3241 tgatattgtc gataaccgta ttactgccga atcgtttagt gacgttgcgc agttcaacaa
     3301 aatttttctg actcatagtg cgctccatta cgcctggttt ttggctttag aacgggagat
     3361 acgagcttct ccgatcagcc agtcaaagag gaaaataatc gccagcatca ccacgatcag
     3421 aatcgaacca taggcgatgg ctacgccata ttcgccgtct tcaacgcggt taagaatgta
     3481 ggcggttgcc acgcgggtgt ccggcgtgac gagaaagacg atggcgctga ccgtcgtgat
     3541 ggcccgcaca aagctgtaga tcagggccga gagaatggcc ggacgcagca gcggcagtaa
     3601 aatgtgggtt atggttcgca aggaacccgc gcgcaggctg agtgaggctt catccagcga
     3661 tttgtcgatc tgccccagac ccgcaatccc ggcgcgaata cccaccggaa cgttacgcat
     3721 caccatcgag ataatcacaa ttgccgccgt tccggtgaga taaaccggag cgctgttaaa
     3781 ggcaagaata tacgaaacgc ccgccaccgt acccggtacg gcaaagcaca gcatggtggt
     3841 gaactcgatg gtctttttgc ctttgaattg ttgacgcacc accacccagg cgatgagcag
     3901 gccaaagata gcggtaatgg gagcggcaat cccggcgtag agcagggtat ccagcagtga
     3961 aggccacgcg ccgtcgctca ttccctgacc aaatagcttg ataaagttat ccagcgtcag
     4021 ggtgtagtcc acgccccagt tcaccgtgaa gctgccgtag aaaatgctgc cgtagagcag
     4081 ggcgttaaag gcaatccata tcgcaagtat ggcgaccacg ctccacacca gggtgaccgg
     4141 tagcggctgg acgtcgccac ggtaggattt gccggaaacc gtgacgtaag agcgtttacc
     4201 aatccacata tactggatgc agaacaccag cagcgagaac agcagcagga aagcgccaag
     4261 ggtgctggcg gcctgataat ccagctgcga gccggtaata tagaagtaaa tttgcgtcgc
     4321 cagtacgtcg aagttaccgc caagtaccag cgggttactg aaatcagcca gtgactgtac
     4381 cacgacgatt aaaaaggcat tggcgagtgc gggctttagc aacggcacaa acacgccgtt
     4441 aaaggtttgc cagcggctgg cgcgcagggt gtaggaggcc tcttccagcg agggatgaat
     4501 ggttttaatg gccccgtcga ggatcataaa tgccattggc gtgaatgcca gaacctgtgc
     4561 cagccagatc ccggtaaaac cgtacagcca gttggtgttg gtcagcccaa accactccac
     4621 cataaattcc gtaacatagc cggagcgccc catcatgagc gtcacgccta agccgaccac
     4681 aaacggtggc gtaacgatgg gcaaaatgga aaagatgcga ccaataatgg cgctgcgttt
     4741 agcgatgcgc gtggtataaa tcgccagcac caggccgaag aaagtacagc ctatccccac
     4801 cgctatcgac aagacgatag agttgacgat cacctgaaca atatgtgcct gcgagagcac
     4861 ggtcataaac gccagcgggg cgaactctcc cgcgtcgttg gtgaacatcg ggataaaaat
     4921 ggcgatgctc ggccagacga taaaaatgcc aatcagcgcc acaatcgcaa tcagtgagcc
     4981 gatcacgaag cggtcgccgc ccagccattc gaggcgggtt aaggccagcg tcataatcgc
     5041 gcctagttca acaaacagca cgatggcggc gtagcctaaa ccgcgtccag ccacggtggc
     5101 gctgatgacg ataaacgcca tacagagcaa tgcccagctg gcatcaaaat aatgacgact
     5161 acgctgttcg cgtgtgacct cacgaaacgg gcgaaccagc aacagactcg gtaacaaata
     5221 ccacagccag ctgatattaa aatgcgacca gccataagcc tcgaggatct catcactcgt
     5281 ggactccagc aggccataat cgaggctcca gctcggcaac agtataaatg ccagccagcc
     5341 cagcaaaacc caaaggaaaa tcgcatcccg ctttttcacg ggatggagtg caagtgtctg
     5401 tgacatagca gttcctgtat ggttggccgg ggcaggcgtt tgtgctgcac ccggcaaata
     5461 cgcctaatgc gggtttattt acccatcttc acttcgctga cccatttatt aatcaatgcc
     5521 ttacgaacat ccgtggaacc atatttatcc atgtcgtagt tgattaattt caggtcgtcg
     5581 agcttcagcg aatttggcga cgtttcggcg gtggtattgg tcaggatctg ataagacttc
     5641 cctttcttcc acgccagttc ctgagcttct tttgataata cccagtccac aaacagtttg
     5701 gcgttgtcga ggttgcgcgc gcctttcaga atgctgacgc cgccaatttc gtagcccgta
     5761 ccttcacacg gggaaatcaa ctccagcggc gcgccctgtt ctttttccag cgagtagtca
     5821 tgcaggaagc caataccaat cgccgtttcg ccacgggcgg cgttacgtgc cggggctatg
     5881 cctgatttgg tgtactgaga gacgttggcg ttcagttgtt ttagataatc aaaggcctga
     5941 tcttcccccc acagctgcgc aaaagtagcc agggcggtgt aggcagtacc ggaactttgc
     6001 ggatcggcaa tttgaatttc gcctttatat tccggtttgg tgagatcttt ccagcacttc
     6061 ggcaccggca ggtttttctc tttcagacga tcggtgttta cgccaaaacc gaggatcccc
     6121 acataaacgg cagaggacag attccctttc acttttgccg gatcgcggaa tttctccatg
     6181 atctgctcaa ggttagggga tttatacgcc tgtagcaggc ccatctcgcc agcctgcgat
     6241 tgcgggtcta aagtgccgcc gtaccagacg tcagcctggg ggtttttctt ttcagcatcg
     6301 acttttgcca gggtgcttcc ggaaccgttg cggataaacg aggtttttac atcatatttc
     6361 tcgccaaacg ctttggtttc ggcctcacac atttcattgg tggcgctgca atagaccacc
     6421 agccggcctt ttgcctgcgc ggcacccgtt aaagtcgcca gcgcaacggc agaagcaatc
     6481 agagtagagg taaaggtcat tttcattgtt ttatccttcg agcgtgagtg gtggtgatgc
     6541 ccgccatcag taacggcatc agcagtaaac ccataagtac tgcggcgacc gagagtaaac
     6601 tgaacattcc cggccagccg tagcgttcga tgaccagcga cagcggccag cctgccagcg
     6661 ccgcgccaag gtaagcgaac aggccgagaa atccggtgat tgaacccgcc gcagctttgt
     6721 gcccacactc caccgccgca agtccaatca gcatttgtgg gccaaagacg aaaaagccca
     6781 cggtaaaaaa gcacactgcc agcagagcgt agtgatgaac cggtgccagc cacagtgcgg
     6841 cgactgacac catcaacccc aacgtgaaca gcaaaatcat tggcgctcgt tgcccgctaa
     6901 acagcaaatc cgatccccat ccggcaaata acgcgccgag taagccgccc acttcaaaca
     6961 gcattaccgt ggcgttggcg ctgagcaggt tgacaccgtg gctttccgtc agccagatat
     7021 tgccccagtc gttaagcgca atccggatca cataaaccag tacataagag acgcccagta
     7081 gccagatcag cggattttgc agcatggtgg tgcgcaacat ttgccacagc cccatcggcg
     7141 gactttgttg ttcctgacgt aactccagcg gatcgtgacg ccagtgacca actgtcggta
     7201 atccctcttc ctggggcgtg cctttgagtt gtagcgttaa ccagatccct aatgccatgc
     7261 tgattatccc cggtgtcagc atcgcggcct gccagcccca ccagtgggcg gcaaaggcgc
     7321 taatcagcgg aataatcgct ccgccaatgt tgatcgacat attccagcag ccccaccaga
     7381 aaccgcgctc attgcgtgag taccagtggg tcagcaagcg ggcacacggc ggccatcccc
     7441 agccctgaaa aaaaccgttc agtgtccaga ccacaagcag aagcgtaagt gattcgccaa
     7501 aggcaaacac cacgttcaat aacccggtgg cgaacaagcc gacgcccata aaaccgcgtt
     7561 gtccgtggct gtcatgccac agtccggcgg taaatttcga caatccgtag ctgagataaa
     7621 acagcgaacc cagcaggcca atgtcgcctt tatccagtcc gaggtcggtt tgtagcgccg
     7681 gaaggacata attcacgctt tttcgcgtca gataaaatgc ggcgtagcca ataatcatgc
     7741 acatcagcaa tcgcggacgt aatgtccggt agcattgatc aatttctctc gctgaacgcg
     7801 cgtgcatggt tgtctccatg ttgctgacta tatgaaaatg aaagtgaaag gggatgagag
     7861 aaaatccggg atacctaaga ctttttcctg gttatccggc gatttgttgc aaaaatgtgg
     7921 gcaagttaac aattacccgc gtgccgtgct gtttttcgag cgttaagtcg ccgcccaggg
     7981 cgctgacgcg ctcacgcatc ccctgaatgc caaatccggc cattttcccg ctggcgatcc
     8041 ccacaccgtt gtcgctgacc tccagatgca atacctctcc ctgctggcgc aggataatcg
     8101 tcacttcgct ggcttcggcg tgtttacaga cgttattgag caactcctgc aataaccgat
     8161 acaaggtaaa gcgcaccgtt tcgttttctg gtgtggagtt cagttgatag gcaaactggc
     8221 aatgaatgcc gcgttcacca aaggcaaatt cattaatcag atgcaacaac gcttcgcgaa
     8281 atgtcagttc atcaagcgcg ggaggccgca actggcgcag caattggcgg gtggagagat
     8341 gaatgcggcg ggcgagctca ttgatttggc tggctgcgga ttgaatttgc gccggatcac
     8401 gcgcccgctt caccagttgc gactggatct gaatggcggt gatgttctga ccgatttcgt
     8461 cgtgcagctc tcgcgccagg ttttttcgcg tgtcctcctc ggtgtggatc aacttttccg
     8521 ccaatgcccg ccgcgctgcc agctcggcct ccagccgttg tcggtaatgg tgcaggttta
     8581 gcgccagatg ttgctggcga ctgatggcaa tgcccaggcc aattcccagc aaggcctggg
     8641 tcgcgaggaa aatctccagc tccaccaggt tactaaagcc cacgccaatc tggcgggcga
     8701 tggtgatcat catgctgccc aaaagcccgg agagtacgcc accttgccag ccaaatttcc
     8761 acgccatcac cacattgggc aaaaagacca cgatcaacag cagacgctca atttcaggtg
     8821 acagcaccat ctgagtgccg ataccaatga taaaaaacag gctacaccag atgattaaag
     8881 aggtacgcaa ggggggatta tgcgtatcga gtcccaggag ctggtagcga tgctgctggc
     8941 gcagaaactc aaagataaga tagacaaacg gcgtcagtaa tacgccgccg gtaaatgagg
     9001 tcaaacccgg cagcattatt gggcctgaca taaaagggga gagcagggcg gtattcaaca
     9061 atgaagctgc cgtgacagtg gctaacagca acgtcagacg ctgccagtaa agtggaaaac
     9121 ggtgccagaa gttttgcgca aggaggacag gaaacaggct aataaccggc gttgccagca
     9181 tgagataacc gttcaatagc tgctcactat ggagccagaa catcatcagc aaagggggga
     9241 gtaccaacgc aggccagtag cggcgagaga gcaaaataac aagggcgaga taaactccct
     9301 gcgggagaaa caacaccgcc tgttgaccgt tattggtcag ataaaagctg agcgtccaca
     9361 gcatcagcca gccggttccc caggccaaca caataaacag cgaaatcacc caatgacgaa
     9421 gattgcgcga cattaatgcc ccgccagcag atggtggtcg agcgcaaaat gtaccagttc
     9481 aatggtgcta tggcagttca gttttcccag cacattggcg cgatgcacat gtaccgtttt
     9541 atgactgagt tcgagtttaa aggcgatctc tttaacgctg tctcctttga caagtaaatc
     9601 aaacacttca cgttctcgtg gggtgagaat ttccagcgcc tgggcgggtt gcccgccgcc
     9661 gcgtaaagcc cgaatcgcat cggcacagag ataatgcccg cccagcccga ccgagcgcac
     9721 cgcctgcact aactcttccg gcccacagcg tttggttaaa tacccgctgg caccggcatc
     9781 cagcgcgctt tgcacaaagg ttggcgcgtc ataaatgcta agaataatgg cgcgaaattg
     9841 cggtttttgt gcacgcaggc gcttaagcaa actgaggccg ttttcgtccg gcatggcaat
     9901 atcaatgacg gcaacattga cgtcatcacg gattaatgcc gaccatgcct gcgcggcact
     9961 actgtactga ccaatgacct ccagatcgtc ttccagagag agtaattgtg cgaagccaga
    10021 gcgcaccacc acatggtcat ccaccagcac tatccgaatc atacttttct tctcttatta
    10081 tctaattctc ctatcatgca tcacggcgcg gcgctggcaa tcaaccagcg acagatatgg
    10141 aatggatgta acaaaatggc aataatttgc cggagcgcgc acagcttatc tggcttgtga
    10201 accggcggga tacgttcgcc aaaaccgcaa aatgcgcaaa ctcgctaacc attgccgatt
    10261 tttttctaaa aacgctttgt tcttaacaag accttttaat tcctttatca aactgacccg
    10321 caacgaaata ctgctcccat aacaagacag gggagcagac aatcatggca atttcatcgc
    10381 gtaacactct tcttgccaca ctggcattca tcgcttttca ggcacaggcg gtgaacgtca
    10441 ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgat aacacctttg
    10501 ctaaagaaag cggagcaact gtggactggc gtaagtttga cagcggagcc agcatcgtgc
    10561 gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca ttagcggttg
    10621 cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg ggtaactccg
    10681 aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggc aaacgcattg
    10741 ccgtaccgtt tatctccacc acccactaca gcttgctggc ggcgctgaaa cactggggga
    10801 ttaaaccagg gcaagtggag attgtgaacc tgcagccgcc cgcgattatc gccgcatggc
    10861 agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc ctggaaaaag
    10921 acggcaaggt gttgaccgat tctgaacagg tcgggcagtg gggtgcgcca acgctggacg
    10981 tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa gcgttcgcta
    11041 aaagcgccat cgatgctcag caaccgtaca ttgctaaccc agacgcgtgg ctgaaacagc
    11101 cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc ggaagatgac gttccggggc
    11161 tggtgaaggg gaatacctat ctgacgccgc agcaacaaac ggcagaactg accggaccgg
    11221 tgaacaaagc gatcatcgac accgcgcagt ttttgaaaga gcagggcaag gtcccggctg
    11281 tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag gaggcgcaga
    11341 tgctgcaaat ctctcatctt tacgccgatt atggcggcaa accggcactg gaagatatca
    11401 acctgacgct ggaaagcagc gagctactgg tggtgctggg gccgtccggc tgcggtaaaa
    11461 ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc attcaactgg
    11521 cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag aatgaagggc
    11581 tactaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg gcaggtatag
    11641 agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg ctggaaggcg
    11701 cagaaaaacg ctatatctgg cagctttccg gtggtcaacg tcagcgggtg gggattgccc
    11761 gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt gcgctggacg
    11821 ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag acgggcaagc
    11881 aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact gaactggttc
    11941 tgctttcatc cggccctggc cgtgtgctgg agcggctgcc gctcaacttt gctcgccgct
    12001 ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc gccatgcgcg
    12061 aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg tgctcattaa
    12121 tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc aggtgacctt
    12181 aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg cgctgcaact
    12241 gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc tcaccattgc
    12301 cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca gcctgacgcg
    12361 cattgtgctg gcgctacttg cggctgtgtt gatcggcatt ccggtcggga tcgccatggg
    12421 actaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc gtccggtgcc
    12481 gccgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa cctcgaagat
    12541 cttactgatc tatttagcga tttttgcacc ggtggcgatg tcggtgctgg cgggggtgaa
    12601 aagcgcgcag caggttcgca ttcatgccgc ccagtcgctg ggtgccagcc gtgcgcaggt
    12661 gctgtggttt gtcattttgc ccggcgcgct gccggagatc ctcaccggat tacgcattgg
    12721 tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga cacgcggttt
    12781 aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac tggcggggat
    12841 cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt tacagcgccg
    12901 tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagtattac cccgctgggg
    12961 ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag cgataatcag
    13021 tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tgtttctgcg cgatcaaact
    13081 attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca tattcaccct
    13141 gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca taacgataat
    13201 ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc acccgcaggg
    13261 gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg gaccagcggt
    13321 attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagcgg gctgcgtgcg
    13381 gagcatgatt tccgtaaatc gttcccggaa tacaaatacc gcaaaaccga ggaggaacat
    13441 caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt ggtgcgaacg
    13501 catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac gcgaattgtc
    13561 gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca tatcactaaa
    13621 ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg ggataaccgc
    13681 gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat gcatcgggcg
    13741 acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg acggttccct
    13801 cgcccccttg gggagagggt tagggtgagg gggcgttcac cgtactttca acaggttaac
    13861 tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca aatccagcgc
    13921 aaagtagctg aaaatcagat ccgcacccgc acgcttaatc gaacctaagc tttcgagcac
    13981 gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg cgtactcacc
    14041 gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac gcacgatgtc
    14101 gaggtatgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt catccagcag
    14161 tgattcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt tgcggtcgcc
    14221 ttttaatgcg cttccggcag cttcacggaa cgggccataa aaggaagagg cgaacttggt
    14281 cgaatacgac ataatcgccg tatctttaaa gcccgcagcg tccagcgcct gacgaatcgc
    14341 ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg cagcagctgc
    14401 aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc catgctcgca
    14461 cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg acatgacgat
    14521 catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc catcttcccg
    14581 ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccgaaagtca tcacggaacg
    14641 aataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctcag gaatgcgcat
    14701 cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa caaagatcgg
    14761 caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca gcgcaggaga
    14821 tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga tgtttgtgga
    14881 attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt tgtcgcgatg
    14941 ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat agcaataatt
    15001 tcata
//
LOCUS       DAJSKJ010000092        14986 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000092 DAJSKJ010000000
VERSION     DAJSKJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.092"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ON345_002416"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ON345_002416"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR3072466.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="ON345_002417"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="ON345_002417"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR3072467.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="ON345_002418"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="ON345_002418"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3072468.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="ON345_002419"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="ON345_002419"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR3072469.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="ON345_002420"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="ON345_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR3072470.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="ON345_002421"
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="ON345_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR3072471.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5724..5885
                     /locus_tag="ON345_002422"
     CDS             5724..5885
                     /locus_tag="ON345_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR3072472.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="ON345_002423"
     CDS             6007..7080
                     /locus_tag="ON345_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR3072473.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="ON345_002424"
     CDS             7077..7859
                     /locus_tag="ON345_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3072474.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(7972..8835)
                     /locus_tag="ON345_002425"
     CDS             complement(7972..8835)
                     /locus_tag="ON345_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR3072475.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8807..10357)
                     /locus_tag="ON345_002426"
     CDS             complement(8807..10357)
                     /locus_tag="ON345_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR3072476.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADADLKQVSPNLTFIYDPEITPDDLLLKVAKNICECSKPHIAN
                     GPVNDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            10615..11394
                     /gene="deoC"
                     /locus_tag="ON345_002427"
     CDS             10615..11394
                     /gene="deoC"
                     /locus_tag="ON345_002427"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3072477.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11521..12843
                     /gene="deoA"
                     /locus_tag="ON345_002428"
     CDS             11521..12843
                     /gene="deoA"
                     /locus_tag="ON345_002428"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR3072478.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            12895..14118
                     /gene="deoB"
                     /locus_tag="ON345_002429"
     CDS             12895..14118
                     /gene="deoB"
                     /locus_tag="ON345_002429"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3072479.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14198..14917
                     /gene="deoD"
                     /locus_tag="ON345_002430"
     CDS             14198..14917
                     /gene="deoD"
                     /locus_tag="ON345_002430"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3072480.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDTREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3561 a         3894 c         4071 g         3460 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccgga taaggcagga aggcgttcgc tacaattcgc agctcgccgc cactattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacaccgtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acacccgcgc cacagccgac atccagcact ttacctttcg tgtgcggggt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat ccgcatcaaa
      841 tactggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat ctgcgacgtc
     1081 atccgccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacaa atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggcgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcttc
     2101 aaacgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gtttcgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgctaaaatc
     2581 ggcatcatca ccaacggttt tagcgccttg cagcaagtgc gtctggaacg gacgggcctg
     2641 cgtgattact ttgatttgct ggtgatttcc gaagaagttg gcattgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacttgc
     2821 tggctgaatg cgcaccatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttca
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggacgc cggtaaaact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgctgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgcta gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac agcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggcacct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaacatg gacccgaaac atcgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gtgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgcag ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 aacgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatccggacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cacgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttatttat cacccatcgc ttttgtgatg gcggccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat tcccaggcac
     5521 agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtatatct gcaatcagcc cggttacgcg
     6241 cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag tcagatgcca
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gccgaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgattg acgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgacacc cactgccatt ttgatttccc gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgt gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattgggctt gcatccaggt atgttggaaa aacatagcga tgtgtctctt gagcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcagt gggggaggtc ggtctggatc
     7381 tcttcggcga cgatccgcaa cttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc tgcattcacg gcgcacacac gacaaactgg
     7501 cgatgcatct taaacgccac gatttatcgc gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg aagcctaacc
     7741 gcccggaaca ggctgcgcgg gtgtttgacg tgctttgcga gttacgtccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcggt gtttagcgtt cgtgggtagt
     7861 ttacccttcg taggcagcat ccggcagtcg gcacataatg cctgatgcga cgcttaacgc
     7921 gtcttatcat gcctacaggt ttgtgccgaa ctgtaggccg tatccggcat gtcacaaata
     7981 gagcgccgga aatatcaacc ggctcacccc gcgcaccttt aacgcatcag ccaacggctc
     8041 aacgtcttcc ggcgtggcgc tcgcccagct ttgcgcctcg ccatacacgc cgtgggcatg
     8101 aaacgcattc aggcgaaccg gaacatcgcc aagtctcttg ataaacgtcg ccagctcatc
     8161 gatgtgatgc aaataatcca cctggtctgg aattaccagc aaacgcagtt ccgccagctt
     8221 accgcgctct gccagcaaac agatgctgcg cttaatctgc tgattatcgc gtccggttaa
     8281 ttgctgatga cattcgctcc cccacgcttt gagatcgagc atcgcgccgt cgcacaccgg
     8341 gagtaatttt tcccagccgg tttcgctcaa cataccgtta ctgtccacga ggcaggtgag
     8401 gtggcgcagt tgcggatcgt ttttgatggc tgtaaatagc gccactacaa acggcagttg
     8461 ggtcgtggct tcgccgccgc ttaccgttat cccttcaata aacagcaccg ctttgcggat
     8521 atggctaagc acctcatcca cgctcatgga ttgcgccatt ggcgtggcat gttgcggaca
     8581 catcttcagg caggtatcac actgctcgca gaccacagcg ttccacagca ctttgccgtc
     8641 aacaatctgc aacgcctgat gcggacactg tggcacgcac tctccgcaat cattgcagcg
     8701 tcccatcgtc cacggattgt gacagttttt gcagcgcaga ttgcagccct gcaaaaacag
     8761 agccagacga ctgcctggcc cgtcaacgca ggagaagggg ataatcttac tgactaaagc
     8821 gcatctgctg ttcatggctt atcacgcgcg gctggcgttc cagaatacga gtgttgcgtg
     8881 cggcttcttc gcccagccag gtggtgttgg tgcgtgaacc ttcggcgcga tatttttcta
     8941 agtccgacaa acgcaccata taaccggtaa cgcgaaccag atcgttaccg ctgacattgg
     9001 cggtaaattc acgcattccg gctttaaagg caccgaggca aagctgtacc agtgcctgcg
     9061 ggttacgttt gatggtttcg tcgagcgtca gaatgtcgct gatgccggaa taataataag
     9121 catgatgcgg ggcgacagtt tgcagatggg tgatcggatc tggctcatcg ccatacggca
     9181 aacgcgcgcc cggcgtggtg ccgatatcgg aactgatccc cgactgtgcg tgtaacatgg
     9241 cgcgtttttg ccagccatat ttcacggggg tattggcgac aaactccgcc agttgcgcgc
     9301 tgatgcgata acccacttca tttgcggcgg cttctttacc gtagcgcgcg gcaatccctt
     9361 ctttctcaca cagcaagtta accgcttccg ccagcccata catgccaaac attggcacaa
     9421 aacgttcagg gttaatcagc ccttctttca ccaggaagct attctcaaag aagtgtgatt
     9481 gttgatagag gaattcacac cgcgcatcga tgatggcgat ctgctgctgg cagtagtgcg
     9541 gtagagtgcg cgtaaagaag tcatccagcg attcgctgcg ctcggcaatg gctttcaggt
     9601 taaggcgtac cagcgtgctg ccaccacccg ccagcggcag tgagttgtaa cagctcacaa
     9661 tcccgtagcc cccttttgtg aaaattttat cattcaccgg accgttggcg atgtgcggtt
     9721 tgctacattc acagatgttc ttcgccactt tcagcagcag gtcatcaggg gtaatttcag
     9781 gatcgtagat aaaggtcagg ttcggtgaaa cctgcttcaa atctgcgtct gcacgtaaga
     9841 tcgcgcgggt aatgggcgaa tcagacgggc cgatattggc gtgcataaag gcgtctggca
     9901 gggttctgtc gaggtaacgc cagaaacgtt ttattcgaac atcgatctcg tcttgtgtta
     9961 gaattctaac atatggttgc aacaacgcat ccagttgccc caggtagacc ggcatcgatg
    10021 tgaccgacgg tacgtggtgg taaagaatgg tcagcagaga gagtgcgtca tcaagatctt
    10081 tcgcgccttc cagctccagc cattcggaac cgttcgccag aaaacgggcg taatcgggta
    10141 agacatagcg cggtttgtac ggcgcatgac cttcaaacat atcgcagatt acaccctcat
    10201 ccagcgcgcg gcgggcttcg gcaggaagct gcggataagg cagattgttt tctgcttcca
    10261 gagccagaaa atggcgcttc tgctccgggc taagcactgg gctggtgaca atttgctggc
    10321 aacgttgttg cagtgcattt tcatgagaag tgggcatctt cttttccttt tatgccgaag
    10381 gtgatgcgcc attgtaagaa gtttcgtgat gttcactttg atcctgatgc gtttgccacc
    10441 actgacgcat tcatttgaaa gtgaattatt tgaaccagat cgcattacag tgatgcaaac
    10501 ttgtaagtag atttccttaa ttgtgatgta tatcgaagtg tgttgcggag tagatgttag
    10561 aatactaaca aactcgcaag gtgaatttta ttggcgacaa gccaggagaa tgaaatgact
    10621 gatctgaaag caagcagcct gcgtgcactg aaattgatgg acctgaccac cctgaatgac
    10681 gacgacaccg acgagaaagt aattgctctg tgtcatcagg ccaaaactcc ggtcggcaat
    10741 accgccgcta tctgtatcta tcctcgcttt atcccgattg ctcgcaaaac gctgaaagag
    10801 cagggcaccc cggaaatccg aatcgctacg gtaaccaact tcccacacgg taacgatgac
    10861 atcgaaatcg cgctggcaga aacccgtgcg gcaatcgcct acggtgctga tgaagttgac
    10921 gttgtgttcc cgtaccgcgc gctgatggcg ggtaacgagc aggttggttt tgacctggtg
    10981 aaagcctgta aagaggcttg cgcggcagcg aatgtactgc tgaaagtgat catcgaaacc
    11041 ggcgaactga aagacgaagc gctgatccgt aaagcgtctg aaatctccat caaagcgggt
    11101 gcggacttca tcaaaacctc taccggtaaa gtggctgtga acgcgacgcc ggaaagcgcg
    11161 cgcatcatga tggaagtgat ccgtgatatg ggcgtagaaa aaaccgttgg tttcaaaccg
    11221 gcgggcggcg tgcgtactgc ggaagatgcg cagaaatatc tcgccatcgc agatgaactg
    11281 ttcggtgctg actgggctga tgcgcgtcac taccgctttg gtgcttccag cctgctggca
    11341 agcctgctga aagcgctggg ccacggcgac ggtaagagcg ccagcagcta ctaagtaaga
    11401 tgctttacgc ctgatgcgct gcgcttatca ggcctacgag acgtatctac ccgtaggccg
    11461 gataaggcgt agacgcatcc ggcaaaagcc gcctcatact cttttcctcg ggaggttacc
    11521 ttgtttctcg cacaagaaat tattcgtaaa aaacgtgatg gtcatgcgct aagcgatgaa
    11581 gaaattcgtt tctttatcaa cggtattcgc gacaacacta tctccgaagg gcagattgcc
    11641 gccctcgcga tgaccatttt cttccacgat atgacaatgc ctgagcgtgt ctcgctgacc
    11701 atggcgatgc gagattcagg aaccgttctc gactggaaaa gcctgcatct gaatggcccg
    11761 attgttgata aacactccac cggcggcgtc ggcgatgtga cttcgctgat gttggggccg
    11821 atggtcgcag cctgcggcgg ctatattccg atgatctctg gtcgcggcct cggtcatact
    11881 ggcggtacgc tcgacaaact ggaatccatc cctggcttcg acattttccc ggatgacaac
    11941 cgtttccgcg aaattattaa agacgtcggc gtggcgatta tcggccagac cagctcactg
    12001 gctccggcag ataaacgttt ctacgcgacc cgcgatatta ccgcaaccgt ggactccatc
    12061 ccgctgatca ctgcctcgat cctggcgaag aaactggcgg aaggtctgga tgcgctggtg
    12121 atggacgtga aagtgggtag cggcgcgttt atgccgacct acgaactctc tgaagccctt
    12181 gccgaagcga ttgttggcgt ggctaacggc gctggcgtgc gcaccaccgc gctgctcacc
    12241 gacatgaatc aggtactggc ctccagtgca ggtaacgcgg ttgaagttcg tgaagcggtg
    12301 cagttcctga cgggtgaata tcgtaacccg cgtctgtttg atgtcacgat ggcgctgtgc
    12361 gtggagatgc tgatctccgg caaactggcg aaagatgacg ccgaagcgcg cgcgaaattg
    12421 caggcggtgc tggacaacgg taaagcggca gaagtctttg gtcgtatggt agcggcacaa
    12481 aaaggcccga ccgacttcgt tgagaactac gcgaagtatc tgccgacagc gatgctgacg
    12541 aaagcagtct atgctgatac cgaaggtttt gtcagtgaaa tggatacccg cgcgctgggg
    12601 atggcagtgg ttgcaatggg cggcggtcgt cgtcaggcat ctgacaccat tgattacagc
    12661 gtcggcttta ctgatatggc gcgtttgggc gaccaggtag acggtcagcg tccgctggcg
    12721 gttatccacg cgaaagacga aaacagctgg caggaagcgg cgaaagcggt gaaagcggca
    12781 attaaacttg ccgataaagc accggaaagc acaccaactg tctatcgccg tatcagcgaa
    12841 taacggtata ctgatctgat catttaaatt tgaagcactg agtacggaga acatatgaaa
    12901 cgtgcattta ttatggtgct ggactcattc ggcatcggcg ctacagaaga tgcagaacgc
    12961 tttggtgacg tcggggctga caccctgggt catatcgcag aagcttgtgc caaaggcgaa
    13021 gctgataacg gtcgtaaagg cccgctcaat ctgccaaatc tgacccgtct ggggctggcg
    13081 aaagcacacg aaggttctac cggtttcatt ccggcgggaa tggacggcaa cgctgaagtt
    13141 atcggcgcgt acgcatgggc gcacgaaatg tcatccggta aagatacccc gtctggtcac
    13201 tgggaaatcg ccggtgtccc ggttctgttt gagtggggat atttctccga tcacgaaaac
    13261 agcttcccgc aagagctgct ggataaactg gtcgaacgcg ctaatctgcc gggttacctc
    13321 ggtaactgcc actcttccgg tacggtcatt ctggatcaac tgggcgaaga gcacatgaaa
    13381 accggcaagc cgattttcta tacctccgct gactccgtgt tccagattgc ctgccatgaa
    13441 gaaactttcg gtctggataa actctatgaa ctgtgcgaaa tcgcccgtga agagctgacc
    13501 aacggcggct acaacatcgg tcgtgttatc gctcgtccgt ttatcggtga caaagccggt
    13561 aacttccagc gtaccggtaa ccgtcacgac ctggctgttg agccgccagc accgaccgtg
    13621 ctgcagaaac tggttgatga aaaacacggt caggtggttt ctgtcggtaa aattgcagac
    13681 atctacgcca actgcggtat caccaagaaa gtgaaagcaa ctggcctgga cgcgctgttt
    13741 gacgccacca tcaaagagat gaaagaagcg ggtgataaca ccatcgtctt caccaacttc
    13801 gttgacttcg actcttcctg gggccaccgt cgcgacgtcg ccggttatgc tgcgggtctg
    13861 gagctgttcg accgccgtct gccggagctg atgtctttgc tgcgcgatga cgacatcctg
    13921 atcctcactg ctgaccacgg ttgtgatccg acctggaccg gtactgacca cacgcgtgaa
    13981 cacattccgg tactggttta cggcccgaaa gtaaaaccgg gttcactggg tcaccgtgaa
    14041 accttcgcgg atatcggcca gactctggca aaatattttg gtacttctga tatggaatat
    14101 ggcaaagcca tgttctgata atgggtgcgg cttgatgccc tcaccccggc cctctcccac
    14161 agggagaggg tgaaaacatg ttgataagga taaaacaatg gctaccccac acattaatgc
    14221 agaaatgggc gatttcgctg acgtagtttt gatgccaggc gacccgctgc gtgcgaagta
    14281 tattgctgaa actttccttg aagatacccg tgaagtgaac aacgttcgcg gtatgctggg
    14341 cttcaccggt acttacaaag gccgcaaaat ttccgtaatg ggtcacggta tgggtatccc
    14401 gtcctgctcc atctacacca aagaactgat caccgatttc ggcgtgaaga aaattatccg
    14461 cgtgggttcc tgtggcgcag ttctgccgca cgtaaaactg cgcgacgtcg ttatcggtat
    14521 gggtgcctgc accgattcca aagttaaccg catccgtttt aaagatcatg actttgccgc
    14581 tatcgctgac tttgacatgg tgcgtaacgc ggtagacgcg gctaaagcac tgggcattga
    14641 tgctcgcgtt ggtaacctgt tctccgctga cctgttctac tctccggacg gcgaaatgtt
    14701 cgacgtgatg gaaaaatacg gcatcctcgg cgtggaaatg gaagcagctg gtatctacgg
    14761 cgtcgctgca gaatttggcg cgaaagccct gaccatctgc accgtgtctg accacatccg
    14821 cactcacgag cagaccactg ccgctgagcg tcagaccacc ttcaacgaca tgatcaaaat
    14881 cgcactggaa tccgttctgc tgggcgataa agagtaattg tgtttcgctg aaaagcgatt
    14941 gtcgtgtgaa gccggagtgg gagactgctc cggcttttta gtatct
//
LOCUS       DAJSKJ010000093        14961 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000093 DAJSKJ010000000
VERSION     DAJSKJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.093"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            129..371
                     /locus_tag="ON345_002431"
     CDS             129..371
                     /locus_tag="ON345_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072481.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            485..1123
                     /locus_tag="ON345_002432"
     CDS             485..1123
                     /locus_tag="ON345_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR3072482.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1120..1857
                     /locus_tag="ON345_002433"
     CDS             1120..1857
                     /locus_tag="ON345_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR3072483.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1857..3953
                     /locus_tag="ON345_002434"
     CDS             1857..3953
                     /locus_tag="ON345_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR3072484.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRTNRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKRAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4000..4278)
                     /locus_tag="ON345_002435"
     CDS             complement(4000..4278)
                     /locus_tag="ON345_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072485.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4548..4958
                     /gene="psiE"
                     /locus_tag="ON345_002436"
     CDS             4548..4958
                     /gene="psiE"
                     /locus_tag="ON345_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR3072486.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5002..6477)
                     /gene="xylE"
                     /locus_tag="ON345_002437"
     CDS             complement(5002..6477)
                     /gene="xylE"
                     /locus_tag="ON345_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR3072487.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6849..7739)
                     /gene="malG"
                     /locus_tag="ON345_002438"
     CDS             complement(6849..7739)
                     /gene="malG"
                     /locus_tag="ON345_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR3072488.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7754..9298)
                     /gene="malF"
                     /locus_tag="ON345_002439"
     CDS             complement(7754..9298)
                     /gene="malF"
                     /locus_tag="ON345_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR3072489.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9452..10642)
                     /gene="malE"
                     /locus_tag="ON345_002440"
     CDS             complement(9452..10642)
                     /gene="malE"
                     /locus_tag="ON345_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR3072490.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11007..12122
                     /gene="malK"
                     /locus_tag="ON345_002441"
     CDS             11007..12122
                     /gene="malK"
                     /locus_tag="ON345_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR3072491.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12194..13534
                     /gene="lamB"
                     /locus_tag="ON345_002442"
     CDS             12194..13534
                     /gene="lamB"
                     /locus_tag="ON345_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR3072492.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13686..14606
                     /gene="malM"
                     /locus_tag="ON345_002443"
     CDS             13686..14606
                     /gene="malM"
                     /locus_tag="ON345_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR3072493.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3711 a         3846 c         3927 g         3477 t
ORIGIN      
        1 ttccaattat cctaaaaccc catctctaat tccccgcgcc gtaattcgca tgctttagtt
       61 gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt caatgaaggg
      121 aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt gcggcgactt
      181 ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg gtcggcagca acgtcggttt
      241 cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg ggtgtcgcgc
      301 tcgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc acgaccacca
      361 gtacccagta ataaagtatg tatccccaaa ataatttgag tcattgcatc tgtggcttga
      421 agtatgaagg gatttaccat aaccacactc cggtgtggtt attctgcccc tctggagaag
      481 agtcgtgaag cgacctgcac tcattcttat ctgcctgcta ttacaggcct gttcagccac
      541 gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag gcgtacagct
      601 gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc ttaatggcgg
      661 gccgcaattg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat gggtcactca
      721 ggatcaagcc actcttgtca cacaacatgg tcgtctggtg aagactttgc ttggcggcga
      781 caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg cacaaattgt
      841 tgatggtgca acatggacgc gcacgatggg ctggaccgag taccagcagg tacgctacgc
      901 caccgcacgc tcagtcttca aatgggatgg caccgatacc gtcaaagtcg gcagcgatga
      961 aaccctggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct ggcataaccg
     1021 ctactggatc gacagcgaag ggcaaattcg ccagtcggaa cagtatctcg gcgcggatta
     1081 tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca aacttttgtc
     1141 gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt caaggtgttc
     1201 agcaatggca gcagtgaggc caaaacacta acaggcgcag agcatttaat cgatctggta
     1261 ggccaaccgc ggctggcaaa cagctggtgg cccggtgcgg tgattagcga agagctggca
     1321 acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga acagggcgca
     1381 gattccagca ccgacgatgc cgccgccatt aacgccttac gccagcaaat ccaggcattg
     1441 aaagtgacag gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt tgccgaacgc
     1501 ggtaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccgcc gtccacggtc
     1561 acgttgttcg ggcttatcag ccatcctggc aatcagccat tcactccagg tcgcgacgtg
     1621 gcgagctatc tctccgggca gaacctgctt agcggtgcgg atcgcagcta cgcgtgggtc
     1681 gtttacccgg acggacgcac gcaaaaagcg ccggtggctt actggaacaa gcgtcacgta
     1741 gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg gagtgagacg
     1801 cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc ccaataatga
     1861 aaaaaagaca tctgcttagc ttactggcgc tgggcattag catggcttgc tacggcgaaa
     1921 catatcctgc gcccattggc ccgtcgcagt cggatttcgg tggcgtagga ttattacaaa
     1981 cgcccaccgc acgcatggcg cgggaagggg agttgagcct gaactatcgc gataacgatc
     2041 agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca acgctgcgct
     2101 acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc gatcaaacgt
     2161 ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac tggctgccgc
     2221 aagtggcggt tggtgcgagg gatatcggcg gtacggggct gtttgatgcg gaatatcttg
     2281 ttgccagcaa agcctggggg ccgttcgatt ttacgctcgg cctgggctgg ggatatctgg
     2341 gcaccagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat tgctatcgcg
     2401 ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac ggtcctgcct
     2461 cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg aaactggagt
     2521 atgaaggcaa taattatcag caggattttg ccgggaagct ggagcaaaaa agtaagttta
     2581 acgtcggtgc gatttatcgc gttaccgatt gggccgacgt taaccttagc tatgaacgtg
     2641 gcaacacctt tatgtttggc gtcacgctgc gcaccaactt taacgatctg cgcccgtctt
     2701 acaacgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt ttgcagcatt
     2761 cggtggtggc gaatcagtta acgctgttga aatacaatgc cggacttgcc gatccacaga
     2821 tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat cgtgattcgc
     2881 gcgaagggat catccgtact aatcggatcg tgatgaacga tctgccggat gggatcaaaa
     2941 cgatccgcat tacggaaaat cgtcttaaca tgccgcaggt gacgacggaa accgatgtcg
     3001 ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgaag cgggcgcaaa
     3061 aacgcgtcga gccagtggtt ccgcagtcca ccgagcaggg ctggtatatc gacaaatcac
     3121 gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg gaaaactttt
     3181 acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac catctgctga
     3241 ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac tacaccaatc
     3301 cgccgcagga ctcgcactta ccgcgcgttc gtacccatgt gcgcgagtat gtgcagaacg
     3361 atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac ggcttctacg
     3421 gtcaggtcta tggaggttat ctcgaaacca tgtttggcgg tgcgggggca gaagtgttgt
     3481 atcgtccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt aaacagcgcg
     3541 actggcgtag tgcaaaagat atgatgaaat tcaccgacta cagcgtgaaa accggacatc
     3601 tgaccgcata ctggacgcca tctttcgccc aggacgtgtt ggtgaaagcg agcgtcgggc
     3661 agtatctggc aggggataaa ggcggcacgc tggagatcgc caaacgcttt gatagcggcg
     3721 tggtggttgg cggctatgcc acgatcacga atgtttcgaa agaggagtac ggcgaagggg
     3781 acttcaccaa aggcgtgtat gtctctgtgc cgttggatct cttctcgtct ggcccgacac
     3841 gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag caacttgggc
     3901 gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc taagtcatgg
     3961 gaaaggtgcc agttttcgca atcactggca ccctcattct taatggcctg ttgagccatc
     4021 aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac attctgtctt
     4081 cctgtccgtc gatatcggtt gcagatctgg ttgcctgagc gactgggcca tcattgggct
     4141 ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac cagcataagc
     4201 aggaagaaca gagactgata acatcaaagc agcaaaataa ggcttcattt ttaccacctt
     4261 tatcaggtta cgtttcattt gttccagagg aacatggtct atttttcgcg cattgctggt
     4321 ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg gtttgtcttg
     4381 ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc ggctttggtg
     4441 gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga tctccgtcac
     4501 atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg acgtcactct
     4561 ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat cttggcctgt
     4621 tgtgcctcgg cctgattttg gttgtcttcc tcggcaaaga aacggtgcat ctggcagatg
     4681 tgctgttcgc gccagaacaa accagcaaat atgagctggt agaaggactg gtggtttact
     4741 ttctctattt cgaatttatc gcgctgattg tgaaatactt tcagtccggt tttcacttcc
     4801 cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc atcgtcgatc
     4861 ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg gtgatcactc
     4921 tgtggttgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg ccgaactggc
     4981 gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct tcgtttccgg
     5041 ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga caaatttcca
     5101 cataaatagt gctgccagaa cgcccataca cccgtaaatc cagtaggaga aaccgttgtg
     5161 gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg agacgaagta
     5221 gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttaccacgaa tggcattcgg
     5281 gaagatttcc gacagcagaa cccagcatac cggaccccag gacatggcaa aggcggcaac
     5341 atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa acgcggtacc
     5401 gaggctaaac ataccgattg ccattccgag cgcgccgata atttgcagtg gcttacgacc
     5461 aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa ctccgacaat
     5521 aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca cttccggcgc
     5581 gtagtacaac accacattga tgccgacaaa ttgctggaag atggagagca taacgccgat
     5641 tacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc catgatccag
     5701 ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa ttttgcgcag
     5761 gatactttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac tttctggcac
     5821 ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa acatataacg
     5881 ccagccgtca gtattcagcc agctggcatc accggaacgg gcaataaaat agtttacgca
     5941 gtaaactaaa agttgcccga aaataatcgc aaactggtta aatgaaacca gttttccgcg
     6001 aatatgagct ggagccagtt ccgcaatata cattggtgaa agcattgagg ctaaaccaac
     6061 gccaataccg ccaataatac gataaataac aaattccggg acataacctg ccagataaat
     6121 aggtactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg cagaacctac
     6181 accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacggc cgaagcggtt
     6241 actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc tggctacgca
     6301 aaatcctaac agggagttgg cagcggattc acttaagttt tgtggagcaa caaagacggt
     6361 attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata ataaaccacc
     6421 taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg tattcattca
     6481 gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat tgtcaggcag
     6541 ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt gatcttaatt
     6601 gtgataatta tcaaaaattg atgaaaacaa tactattgcc gtgactcaga gcacgaaaga
     6661 gaattatcgt aagtgggaaa acaaataacg taaaaataat aagttctatg atgggaatat
     6721 taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt ggagcgccga
     6781 ataatagtca caagttggga taacgtaagt tgagggtgca gcggcataac attggcagaa
     6841 caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca gcgttgagcc
     6901 agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc aaagtcaccc
     6961 cacaggtagt tttgcgggtt gaggtattgc tgcatcccca cggccagggt gtagctgttt
     7021 acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat aaacgacagg
     7081 atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg gaaggcctgc
     7141 cacggtgtcg caccatccag cgcagccgct tcttccagcg aactgtcgat ggtttcgaaa
     7201 tagcctttga tggtccagac atgcagcgca atcccaccca gatacgcgaa aattacgccg
     7261 ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa caacgcatac
     7321 aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc tttcagcagc
     7381 gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt ggagagcgcc
     7441 acaatgccaa tcgcggaaat cccagcgacc tttaccgagt tccacagcca cagcagtacc
     7501 gggaatggcg gtggcgtaat gcgaccatca gcctgttcaa cgctaaaacc taacgccagt
     7561 ttccagtgat cccaggagat ttgctccggg atcaggctac cggtcgcgaa gtttccctga
     7621 cgcagcgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat aaaaagtagc
     7681 agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac cattgccatt
     7741 tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact atcgccagcg
     7801 cacccaccag caggaagatc agcgtggcaa ttgctgccgc cagaccgaag tcctgacccc
     7861 cgccgccttc aaaagcgatg cggtaagtgt agttaacaag caggtcggta taaccggctg
     7921 gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagctgaatc agcacgaagt
     7981 tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta atcagcagcg
     8041 gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct gaggcttcat
     8101 acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg tacggataac
     8161 ccagccaggt attgacgata atcagcatcg ttcgggcagt ggtcggatcg ctgaaccagg
     8221 cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ttcaccgaag ctctggttaa
     8281 acaacccttt gaaaatcaag attgaaatga atgatggcac cgcgtagggc agaatcagca
     8341 ggacgcgata taccgctttg ccacgcaacg cttcccactg caccagacac gccagaacca
     8401 tgccgaccgc caccgttaaa aagacggtga tcagtgaaaa caccacggtc cagacgaaaa
     8461 tggcgaggaa cggtttctga atgccttcgt cggtgaagac gcgggtaaag tttttccagc
     8521 cggtggtcac ggtgtaaccg gggcttagct tttcatcccc ccagttgccg tcggcggtaa
     8581 tggactggta aaagccaatt tggttattcg gacgatattt cacgccactc tgattattcg
     8641 tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac tggcgcaggg
     8701 agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc agcgcctggc
     8761 ggttctgggt aatcacgcgc agattcgcgc gttcgccttc gggctgggcg gtcgtttctt
     8821 tcagttgcag tttttgctcg ccgccaaatt taaacgcgtc ggagaggtaa tttttaccgg
     8881 tttcgccgtc gctgagcgcc agttgccact catcgcccgc cgggtaaaga ccaaagttat
     8941 aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgcgcacgt tcaaaagtca
     9001 gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc agagggaaga
     9061 ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg taggctttac
     9121 gattagcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg gcgaacaggt
     9181 attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg agcagaccta
     9241 gcactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg acatccatgg
     9301 ggttcttcct cattccagga cggataaggc tttcacgcct tatccgacaa caactgcctg
     9361 atgcgacgct gatgcgtctt atcaggccta catacgtttc ggttttgtag gccggacaag
     9421 gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc tgcgcgtctt
     9481 tcagggcttc atcgacagtc tgacgaccac tggcggcgtt gatcaccgca gtacgcacgg
     9541 cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt
     9601 ccatggtggc ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac ttcagcgcta
     9661 cggcacccag cggtttgtct ttattaaccg cttccagacc ttcgtcagtc agcagatagt
     9721 tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata ccggcgctca
     9781 gcacgccaac gaacggttta gatggttgac ccttgaaggt cggcagtacc gttacaccat
     9841 aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgatg gtcatcgctg
     9901 tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt
     9961 tgtttttaat caggtcaacc aggaaggtca gacccgcttt cgcgccagcg ttatccacgc
    10021 ccacgtcttt aatgtcgtac ttgccgtttt catacttgaa cgcataaccc ccgtcagcag
    10081 caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg ctcttacctt
    10141 tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggc gggttcggca
    10201 gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa gcaatcagct
    10261 tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg aacgctttgt
    10321 ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaaacgg tcgtgcgccc
    10381 agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc tcttccagtt
    10441 tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc ttaccgactt
    10501 cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta ccttcttcga
    10561 ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat gcgaggatgc
    10621 gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct cgtgaaaaca
    10681 cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt gcccctacgc
    10741 cccaccgttg ctttgtgtga tctctgttac agaattggcg gtaatgtggg gatgcgcaca
    10801 taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac ctcggtttag
    10861 ttcacagaag ccgtgttctc atcctcccgc ctcctccccc ataaaaaagc cagggggtgg
    10921 aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt gctgtcgatg
    10981 acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat gtaacgaaag
    11041 cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa ggtgaattcg
    11101 tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg attgccgggc
    11161 ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat gacactccgc
    11221 cagcagaacg cggcgttggt atggtgtttc agtcttacgc gctctatccc cacctgtcag
    11281 tagcagaaaa tatgtcattt ggcctgaaac tggcaggcgc aaaaaaagag gtgattaacc
    11341 aacgcgttaa ccaggtggcg gaagtgctgc aactggcgca tttgctggat cgcaaaccga
    11401 aagcgctctc tggcggtcag cgtcagcgtg tggcgattgg ccgtacgctg gtggccgagc
    11461 caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg cgtgtgcaaa
    11521 tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt tacgtcaccc
    11581 acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac gccggtcgcg
    11641 tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt tttgtcgccg
    11701 gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc accgcaatcg
    11761 atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg ccagttgaaa
    11821 gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa catctactgc
    11881 cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag caactcggca
    11941 acgaaaccca aatccatatc cagatccctt ccattcgtca aaacctggtg taccgccaga
    12001 acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg ccagagcgtt
    12061 gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag ccgggcgttt
    12121 aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga aaagcaatga
    12181 ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg ccgtcgcagc
    12241 gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac gttccggtat
    12301 tggctggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg ctcaaagtaa
    12361 ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc aggaagtgtg
    12421 gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg tcgcgcaaca
    12481 gaatgactgg gaagctaccg atccggcctt ccgtgaagca aacgtgcagg gtaaaaacct
    12541 gatcgaatgg ctgccaggtt ccaccatctg ggcaggtaag cgcttctacc aacgtcatga
    12601 cgttcatatg atcgacttct actactggga tatttctggt cctggtgcgg gtctggaaaa
    12661 catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg aagctggtgg
    12721 ttcttcctct ttcgccagca ataatattta tgactatacc aacgaaaccg cgaacgacgt
    12781 tttcgacgtg cgtttagcgc agatggaaat caacccgggc ggcacattag aactgggtgt
    12841 cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg catcgaaaga
    12901 cggctggtta ttcactgctg aacatactca gagtgtcctg aagggcttta acaagtttgt
    12961 tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc agggttctgg
    13021 cgtcgcgttt gataacgaaa aatttgccta caatatcaac aacaacggtc acatgctgcg
    13081 tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt acgtgggtat
    13141 gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg ttggtattcg
    13201 cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct acgacaacgt
    13261 cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg cacaacaatg
    13321 gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa cctacgccaa
    13381 gtgggatgag aaatggggtt atgactacaa cggcgatagc aaggttaacc cgaactacgg
    13441 caaagccgtt cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca gcgacgagtg
    13501 gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc ggataaggcg
    13561 tttacgccgc atccggcata aaaacaggtt gtcattatct gaaaggggcg aaagcccctc
    13621 tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat tttgaggtga
    13681 aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg ttactggcaa
    13741 gcgcgcctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc agcgacgcgc
    13801 cagccattcc atctgctgcg ctgcaacaac tcacctggac accggtcgat caatctaaaa
    13861 cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc atcagtggtc
    13921 cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg ctgaccagcg
    13981 aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat cagaacatga
    14041 ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc gtgatgagtg
    14101 cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa aaactttatg
    14161 ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc gacccggcta
    14221 aagcctatgc caagggcgtc ggtaactcga tcccggatat ccccgatccg gttgctcgtc
    14281 ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc agcgtgttgg
    14341 taggaccttt atttggttct tccgctccag ctccggttac ggtaggtaac acggcggcac
    14401 cagctgtggc tgcacccgct ccggcaccgg tgaagaaaag cgagccgatg ctcaacgaca
    14461 cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat gttgataagg
    14521 cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt tccaccttta
    14581 tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg caacaactgg
    14641 tgcgtctcct ggcgcacctt tttttatgct tccttcctgg gatatgagcg attttttata
    14701 gtaactcact tcttcttcac taagaatatc cattatctca atgccttatc agagattctt
    14761 ttcctttcgc cggtagtgtc tggacattca ggctactttt ccaggttatt ttatttctgt
    14821 tatgcagagg ttttatgata agtcatatcc taaattctgg cggcaataac tctttgatga
    14881 aacatgatgt ggtgcaagga aataatatag tagatcttga tttactacgt aatttcaatg
    14941 gggtgccagg tttaaacaga g
//
LOCUS       DAJSKJ010000094        14853 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000094 DAJSKJ010000000
VERSION     DAJSKJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.094"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..772
                     /locus_tag="ON345_002444"
     CDS             <1..772
                     /locus_tag="ON345_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR3072494.1"
                     /translation="QKYTEDKSRHKDNEHAIFWLKKAALQGHTFASNALGWILDRGED
                     PNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYKQAALQGHSDAQ
                     NNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGEGVDQDYKQAMY
                     WYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDATAWYNLAIMYH
                     YGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(842..1324)
                     /locus_tag="ON345_002445"
     CDS             complement(842..1324)
                     /locus_tag="ON345_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR3072495.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1559..4141
                     /gene="leuS"
                     /locus_tag="ON345_002446"
     CDS             1559..4141
                     /gene="leuS"
                     /locus_tag="ON345_002446"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR3072496.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLHGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4156..4737
                     /gene="lptE"
                     /locus_tag="ON345_002447"
     CDS             4156..4737
                     /gene="lptE"
                     /locus_tag="ON345_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR3072497.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVMIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4737..5768
                     /gene="holA"
                     /locus_tag="ON345_002448"
     CDS             4737..5768
                     /gene="holA"
                     /locus_tag="ON345_002448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR3072498.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5770..6411
                     /gene="nadD"
                     /locus_tag="ON345_002449"
     CDS             5770..6411
                     /gene="nadD"
                     /locus_tag="ON345_002449"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR3072499.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6435..7046
                     /locus_tag="ON345_002450"
     CDS             6435..7046
                     /locus_tag="ON345_002450"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR3072500.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7306..7623
                     /gene="rsfS"
                     /locus_tag="ON345_002451"
                     /gene_synonym="rsfA"
     CDS             7306..7623
                     /gene="rsfS"
                     /locus_tag="ON345_002451"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR3072501.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7627..8094
                     /gene="rlmH"
                     /locus_tag="ON345_002452"
     CDS             7627..8094
                     /gene="rlmH"
                     /locus_tag="ON345_002452"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR3072502.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8125..10026
                     /gene="mrdA"
                     /locus_tag="ON345_002453"
     CDS             8125..10026
                     /gene="mrdA"
                     /locus_tag="ON345_002453"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR3072503.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10029..11141
                     /gene="mrdB"
                     /locus_tag="ON345_002454"
                     /gene_synonym="rodA"
     CDS             10029..11141
                     /gene="mrdB"
                     /locus_tag="ON345_002454"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR3072504.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11152..12228
                     /gene="rlpA"
                     /locus_tag="ON345_002455"
     CDS             11152..12228
                     /gene="rlpA"
                     /locus_tag="ON345_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR3072505.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPAMVTP
                     QAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGPFASK
                     AEASTLQQRLQTEAQLQSFITTAQ"
     gene            12367..13578
                     /gene="dacA"
                     /locus_tag="ON345_002456"
     CDS             12367..13578
                     /gene="dacA"
                     /locus_tag="ON345_002456"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR3072506.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13689..13952
                     /locus_tag="ON345_002457"
     CDS             13689..13952
                     /locus_tag="ON345_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR3072507.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14053..14694
                     /gene="lipB"
                     /locus_tag="ON345_002458"
     CDS             14053..14694
                     /gene="lipB"
                     /locus_tag="ON345_002458"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR3072508.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3650 a         3789 c         3892 g         3522 t
ORIGIN      
        1 acaaaagtat actgaggata aaagccgtca taaagataat gagcatgcca tcttttggct
       61 gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct ggatactgga
      121 tcgtggagaa gaccccaatt ataaagaagc ggttgtctgg tatcagatag ccgcggagag
      181 cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca acggagtcgc
      241 aaaagactat gcgctggcat ttttctggta caaacaagct gcattacaag gccatagtga
      301 cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc aaaacgagac
      361 actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcatg ctcagtttca
      421 aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc aagcgatgta
      481 ctggtatctg aaggctgccg ctcaggggag cgtcggcgct tacgtcaaca tcggttatat
      541 gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa
      601 agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt atcactacgg
      661 agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc
      721 cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt agagataaaa
      781 acaagggccg gggaatttca ttcggcccat attctcttta ttgctgtgcg aaatcagact
      841 attacggctc aaacgggcgt ctctggaact ggtcatgacc acatttcggg catagcgtca
      901 gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca
      961 gccctaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc
     1021 gccattcaag ctgcgtttta tcggtgatgt ctgccagctc ctgccacaag ctttctttaa
     1081 tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag ctcatggcga
     1141 actcttccag gtcacgtctg acagctcgcg tcagctcatc gacctcggtt cgcgttaact
     1201 cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc
     1261 cattgcgcag gcgttcgctc agtgacgcaa ccagttcacg gtaatattga gcaaccttgt
     1321 tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg
     1381 ctttgctaat acgcccacaa actctgtaaa ttaatgtatg aataatccgg gcatcatttc
     1441 aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct atgcggatct
     1501 gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa acaggaccac tggctgccat
     1561 gcaagagcaa taccgcccgg aagagataga atccaaagta cagcttcatt gggatgagaa
     1621 gcgcacattt gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct
     1681 tccctatcct tctggtcgac tacacatggg ccacgtacgt aactacacca tcggtgacgt
     1741 gatcgcccgc taccagcgta tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc
     1801 gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac accgctccgg caccgtggac
     1861 gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg
     1921 gagccgcgag ctggcaacct gtacgccgga atactaccgt tgggaacaga aattcttcac
     1981 cgagctgtat aaaaaaggcc tggtatataa gaagacttct gcggtcaact ggtgcccgaa
     2041 cgaccagacc gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac
     2101 caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga
     2161 gctgctcaac gatctggata aactggatca ctggccagac accgttaaaa ccatgcagcg
     2221 taactggatc ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg actatgacaa
     2281 cacgctgacc gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt
     2341 agctgcgggt catccgctgg cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt
     2401 tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa
     2461 aggcgtcgat actggcttta aagctgttca cccattaacg ggcgaagaaa ttcccgtttg
     2521 ggcagcaaac ttcgtattga tggagtacgg cacgggcgca gttatggcgg ttccgggtca
     2581 cgaccagcgc gactacgagt ttgcctctaa atacggcctg aacatcaaac cagttatcct
     2641 ggctgctgac ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct
     2701 gttcaactct ggcgaattca acggtcttga ccatgaagcg gccttcaacg ccatcgccga
     2761 taaactgact gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg
     2821 tgtttctcgt cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac
     2881 cgtaatgccg accccggacg accagctgcc ggtgatcctg ccggaagatg tggtgatgga
     2941 cggcattacc agcccgatta aagcagatcc ggagtgggca aaaactaccg ttaacggtat
     3001 gccagcgctg cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc
     3061 gcgctacact tgcccagagt acaaagaagg tatgctggat tccgaagcgg ctaactactg
     3121 gctgccggtg gatatctaca ttggtggtat cgaacacgcc attatgcacc tgctctactt
     3181 ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa
     3241 acagttgctg tgtcagggta tggtgctggc agatgccttc tactatgttg gcgaaaacgg
     3301 cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat
     3361 cgtgaaagcg aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc
     3421 caagtcgaag aacaacggta tcgacccgca ggtgatggtt gaacgttacg gcgcggacac
     3481 cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg actctcgaat ggcaggaatc
     3541 cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg aaactggttt acgagcacac
     3601 agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg actgaagatc agaaagcgct
     3661 gcgtcgcgat gtgcataaaa cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac
     3721 cttcaacacc gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccaac
     3781 cgatggcgag caggatcgcg ctctgatgca ggaagcgctg ctggccgttg tccgtatgct
     3841 taacccgttc accccgcaca tctgcttcac gctgtggcag gaactgaaag gcgaaggcga
     3901 tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct
     3961 ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc accgttccgg tggacgcaac
     4021 ggaagaacag gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat atcttcatgg
     4081 cgttaccgtg cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta
     4141 agcgcgggag gaagcgtgcg atatctggca acattgttgt tatctctggc ggtgttaatc
     4201 accgccgggt gtggctggca tctgcgtgat accacgcagg ttccttccac tatgaaggtc
     4261 atgatcctgg actcaggcga tccgaacggg ccattaagcc gtgcggtgcg taaccagtta
     4321 cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccctg
     4381 cgtttgggca aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca
     4441 gcagagtatc agatgatcat gacggtgaat gcgaccgtga tgatccccgg ccgtgatatc
     4501 tacccgatta gcgccaaagt cttccgttcg ttctttgata acccgcaaat ggcgttagcg
     4561 aaagataacg aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg
     4621 attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg
     4681 accacaacgg atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga
     4741 ttcggttgta cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc
     4801 ttttacttgg taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag
     4861 ctgcggcaca aggattcgaa gaacaccaca ctttttccat tgatcccaac actgactgga
     4921 atgcgatctt ttcgttatgc caggctatga gtctgtttgc cagtcgacaa acgctattgc
     4981 tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg
     5041 gacttctgca tgacgacctg ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag
     5101 aaaatgccgc ctggtttact gcgcttgcga atcgcagcgt gcaggtgacc tgtcagacac
     5161 cggagcaggc tcagcttccc cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac
     5221 tggatgacgc tgcaaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg
     5281 ctcaggcact ggagcgttta tcgctgctct ggccagacgg caaattgaca ttaccgcgcg
     5341 ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt
     5401 tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca actgcgtctg gaaggcagcg
     5461 agccggttat tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac
     5521 gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc
     5581 gccggggcat gatgggcgag gcgttaaatc gcttaagtca gccgcagtta cgtcaggccg
     5641 tgcaactcct gacacgaacg gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg
     5701 cagagctgga agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg
     5761 acggttgata tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat
     5821 ggtcatctaa aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaatc
     5881 atccctaata atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa
     5941 cacatgcttg aactggcgat tgccgacaag ccattattta ctcttgatga acgcgagcta
     6001 aagcgcaatg ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaagga
     6061 ccggacgtgc cgctggcgtt tattattggt caggattcac tgctgacctt tccgacctgg
     6121 tacgaatacg aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac
     6181 ccacttgaaa tggcgcaacc gcaataccag caatggctgg aagatcattt gacacataac
     6241 ccggaagatc ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt
     6301 aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta
     6361 ttgccggaac cggtactgac ttacattaac caacaaggct tgtatcgctg atacctgtcg
     6421 ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatatcg
     6481 atggtctata cagcggtcat gcgcccaccc ccctgaccgc gcgcggtatt gagcaagcgc
     6541 aaaatctgca tacgctgcta catggtgttt cctttgatct ggttttatgc agtgaactgg
     6601 aacgggcaca gcataccgcg cgactggttc tcagtgaccg ccagctcccc gtgcaaatca
     6661 tccctgaact caacgaaatg ttttttggcg actgggagat gcgacatcat cgcgacctca
     6721 tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat gcaatcccca
     6781 cgaacggtga aggatttcag gcattttcgc aacgtgtgga acgctttatc gcaaggctta
     6841 gtgaatttca gcactatcag aatattttag tcgtcagcca tcagggtgta ctgagtctgt
     6901 taatcgcccg tttaattggc atgcctgccg aggctatgtg gcattttcgc gttgaccagg
     6961 ggtgttggag cgccattgat atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc
     7021 gtgccatcgg ggtcgagaat gcatgacttt tctgtttttt tacgggtaag ccgcaacgac
     7081 cattgacaga cccgggcagg ctgatattct ccgcagccag acttttttcg ccagacacga
     7141 ctttgtagaa attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata
     7201 gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat
     7261 actgacagac catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca
     7321 ctccaggatt ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta
     7381 gacgttcaag gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc
     7441 cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta
     7501 ccgctcggcg tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg
     7561 attgtccatg tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt
     7621 taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc
     7681 ggttttaccg agtacctgcg tcgttttccg aaagatatgc ccttcgagct gattgaaatt
     7741 ccggccggaa aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag
     7801 cagatgttgg cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc
     7861 tgggatacgc cgcagttagc cgctgagctg gaacgctgga agctggatgg tcgcgacgtc
     7921 agtctactga ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag
     7981 agctggtcgc tgtcgacgct taccctcccc catccgctgg ttcgcgtgct ggtcgcagag
     8041 agtctgtacc gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataaggg
     8101 agctttgagt agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca
     8161 gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc
     8221 ggcgtgctta tcgccaacct gtataatctg caaattgttc gctttaccga ctaccagact
     8281 cgctctaatg aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg cattatctac
     8341 gaccgtaatg gtatccctct ggccctcaac cgcactatct accagataga aatgatgcca
     8401 gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc
     8461 gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt
     8521 ccggtgaaaa ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt
     8581 tttccgggtg tcgaagttaa aggctataaa cgtcgttact atccttacgg ttcggcgttg
     8641 acccacgtca tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat
     8701 gacggcaaac tggccaacta tgcggcaacg catgatatcg gtaagctggg cattgagcgt
     8761 tactatgaag acgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt
     8821 gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg
     8881 acgctggatc tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct
     8941 gtggttgtca ccgatccgcg tacaggtggg gtgctggcgc tggtttccac gcctagttat
     9001 gacccaaacc tgtttgttga cggtatctcc agcaaagatt attccgcctt gttgaacgac
     9061 ccgaatacac cgctggtgaa ccgcgccaca cagggggttt atcctcccgc gtctacagtt
     9121 aaaccctatg tggcggtttc ggcattgagc gccggggtga tcacgcgcaa tacgacgctg
     9181 tttgacccag gctggtggca actgccaggt tcggaaaaac gttatcgtga ctggaaaaaa
     9241 tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag aatctgcaga taccttcttc
     9301 tatcaggtgg cctacgatat ggggatcgat cgcctctccg aatggatggg taaattcggt
     9361 tatggtcatt acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc
     9421 gaatggaaac agaaacgctt taaaaaaccg tggtatcagg gtgacaccat tccggttggt
     9481 atcggtcagg gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg
     9541 attaatgacg gtatcgtgaa ggttcctcat ttgctgatga gcaccgccga agacggcaaa
     9601 caggtgccat gggtacagcc gcatgaaccg cccgtcggcg atattcattc cggttactgg
     9661 gagctggcga aagacggtat gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa
     9721 tactttgcca gcgcaccgta caaaattgcg gcgaaatccg gtaccgctca ggtcttcggt
     9781 ctgaaagcga acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa
     9841 ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat gattctggag
     9901 aacggtggtg cgggtccggc ggttggtaca ctgatgcgcc agatcctcga ccacattatg
     9961 ctgggtgata acaacaccga tctgcctgcg gaaaatccag cggttgccgc agcggaggac
    10021 cattaatcat gacggataat ccgaataaaa aaacattctg ggataaagtc catctcgatc
    10081 ccacaatgct gctgatctta ctggcattgc tggtttacag cgccctggtt atctggagcg
    10141 ccagcggtca ggatattggc atgatggagc gtaaaatcgg ccaaatcgcg atgggtctgg
    10201 tcatcatggt ggtgatggcg caaattcctc cacgcgttta tgaaggctgg gccccctatc
    10261 tctatatcat ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc atctctaaag
    10321 gtgctcaacg ctggctggac ctcggtattg ttcgttttca gccgtcggaa attgccaaaa
    10381 tagccgtacc actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga
    10441 agaacactgg catcgcgctg gtgctgatat ttatgcccac gctgctggtg gctgcacagc
    10501 ctgacctggg aacatcaatc ctcgttgcgc tttccggtct gtttgtactg ttcctctctg
    10561 gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt ggcggcgttc attccgattc
    10621 tgtggttctt cctgatgcat gattaccagc gccagcgcgt aatgatgctc ctggacccgg
    10681 aatcagaccc actcggcgcg ggctatcaca ttattcagtc taaaattgct attggctccg
    10741 gcggattacg cggcaaaggc tggctgcacg gcactcagtc acagcttgaa tttctccccg
    10801 aacgccatac cgactttatc ttcgcggtac tggcggaaga gctgggatta gtgggcattc
    10861 tgattctgct cgctctctac attctgctga tcatgcgcgg gctgtggata gccgccagag
    10921 cgcaaaccac ctttggtcgc gtcatggctg gtggcttaat gctgatatta ttcgtttatg
    10981 tcttcgtaaa tattggtatg gtaagcggta ttctgccggt tgtaggggtt ccgctcccac
    11041 tggtcagtta tggaggatcg gcgctaattg tgctgatggc tgggttcggg attgtaatgt
    11101 caatccacac ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag
    11161 cagtggctcg ggatctgcat cgcggcagga atgctcgcgg catgtacaag cgatgatggt
    11221 cagcaacaga cggtaagtgt accgcagcct gcggtatgta acggccccat agttgaaatt
    11281 agcggggcgg acccgcgttt cgaaccactg aacgcgacgg caaatcagga ttaccagcgc
    11341 gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc gggactggcg
    11401 gcaatctatg atgccgaacc aggcagtaac ctgacggcct ctggcgaagc tttcgatccg
    11461 acacagctga cggcggccca tccaacgctt ccgatcccca gctacgccag aatcactaac
    11521 ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc gcggccctta cggcaacgac
    11581 cgcgttattt cgctttctcg cgcggcagct gaccgtctta acacgtcaaa caacaccaaa
    11641 gttcgtatcg atccgattat tgtcgcccag gatggttcgc tttctggtcc tggtatggct
    11701 tgtaccacag tcgccaaaca gacttacgcc ctgccagcac ctcccgattt aagcggtggc
    11761 gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc ttccggtcag taattcgacg
    11821 ctaaaaagcg aagatccgac cggcgcgccg gtaaccagta gcggtttcct cggcgctcca
    11881 acgaccttag cgcctggtgt actggaaggc agcgaaccga cgcctgcgcc acagcccgtt
    11941 gttacagctc cgtcgacaac gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc
    12001 agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc gcaacagtac
    12061 caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc
    12121 tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt gcagcaacgt
    12181 ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg taaagcaggc
    12241 atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct
    12301 gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga tgtcgtagtt
    12361 cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct
    12421 ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc
    12481 ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa
    12541 gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg
    12601 accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc
    12661 actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg
    12721 ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa
    12781 tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt
    12841 gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg
    12901 gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc
    12961 caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg
    13021 tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc
    13081 gacggcatca aaaccggaca cactgacaaa gcaggttaca accttgttgc ttctgcgact
    13141 gaaggccaga tgcgcttgat ttctgcggtg atgggcggac gtacttttaa aggccgtgaa
    13201 gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg
    13261 aaagtaggta aagagttcgc ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg
    13321 ttaggggttg ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa
    13381 gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc
    13441 ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg
    13501 caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc
    13561 catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta
    13621 agcatcagta aaaaaactcc cgccttctga cgggagttgc tatttaatta cgttacgccg
    13681 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    13741 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    13801 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    13861 tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg
    13921 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt
    13981 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    14041 ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    14101 gagccaatct cccaggctat gcatgaattc accgataccc gcgatgatag tacccttgat
    14161 gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag
    14221 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    14281 tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    14341 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg
    14401 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    14461 tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    14521 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg
    14581 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg
    14641 gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat
    14701 acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat gctttacatc
    14761 tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc
    14821 gtaactcacc ccattgatta acctattgaa ata
//
LOCUS       DAJSKJ010000095        14537 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000095 DAJSKJ010000000
VERSION     DAJSKJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.095"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            82..1101
                     /gene="galE"
                     /locus_tag="ON345_002459"
     CDS             82..1101
                     /gene="galE"
                     /locus_tag="ON345_002459"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3072509.1"
                     /translation="MTILVTGGAGYIGSHTVLALQERNDDVVVLDNLSNASQTSLERV
                     AELTGKKPIFYKGDVMDRHLLKKIFASHNITDVIHFAGLKSVSESIKDPLSYYQNNVT
                     GTLVLLEEMRAAGVNSFIFSSSATVYGNPDRVPLNENSRTGGTTNPYGTSKYMVEQIL
                     EDFSRAQPEFRITCLRYFNPVGAHPSGRIGEDPNGIPNNLVPYIAQVTIGRLEVLSVY
                     GNDYPTPDGTGVRDYIHVMDLAHGHLAALDNKDKGDAYKVFNLGTGIGYSVLDLVNAF
                     EKAAQTKINYRFAPRRGGDIAECWSDPSRARRELGWQATRTIEEMMRDTWNWQKNNPN
                     GYRSV"
     gene            complement(1149..2552)
                     /locus_tag="ON345_002460"
     CDS             complement(1149..2552)
                     /locus_tag="ON345_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HCR3072510.1"
                     /translation="MKHQWMTAIFCLVCPLSSMAAITTVMPTALTGPLTNPGMGVASF
                     HQGYGEKLPLTEYPDTGIEYERFYWRDLEPEEGKYHFARVDDAFKYAAAHRPAMNVGL
                     RFMALAEPGSGSRIPDWLIKKGIKGTWTADRKSFIPDLADPLFITYSQRLLQALGQRY
                     DGNDNLAFVDIGMVGSWGEWHNSNFPELKPLLERNTTEELDRFVNMHFSAFPKTPKVM
                     LISGGNSLVNAVRKGAGWRADCWGDLRMFSSTWNHMADDYPQRLEAAQRAYPGFNDAW
                     KRAPVNLEICGYMAQWQRDQHYTREEVQRIFDWALAHHASTLNLKSRVIPGEYRQIVD
                     NALTKIGYRFRLVSLSHESVGQPGTALTLNSRWVNEGVAPIYLPYTLSFRLVDASGKP
                     VASGESKEDLLRWLPGEYTVDFLLDMPRTMPGGSYFIEVAIVDKSGAPRIRLANEGQQ
                     SSNWYRISKVTVLSSTQ"
     gene            complement(2575..2982)
                     /locus_tag="ON345_002461"
     CDS             complement(2575..2982)
                     /locus_tag="ON345_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072511.1"
                     /translation="MTRSEQRPLVIDRFYEALDEQTAEQLTAQQKAEVEKAIIAITLA
                     SRHRVDFRRSFPFFGKRFYLVFLMGRDMRKRFRHESTLSSLLAAFLLLSGLLFLTFCV
                     LLTLYMIKSALGIDVFHDYHVGIWDWWLSLKNQ"
     gene            complement(2985..4097)
                     /locus_tag="ON345_002462"
     CDS             complement(2985..4097)
                     /locus_tag="ON345_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3072512.1"
                     /translation="MSQKITVLGTRGIPDVQGGVETHCQNLYPAIKKQFDMDICVIAR
                     SPYVSYKQTYYKNVETYSLWAPKKRSLEAIVHSFLATLRTCFDGSDIVHVHAIGPGLL
                     VPLLRVLGKKVVFTHHGPDYDRQKWGRLAKRVLQLGEKVAVKYANEVIVISEVINQLI
                     RTKHCRDDAHLIYNGVNLPLPLKEETVRTVLGRYALQPQNYLVVVGRFVEEKGMHDAI
                     AAHRKLGLTMPLVLVGDADHPTEYSVRLKKMAADTPNVIMTGFLKGEELQAIFSQARL
                     FLMPSYHEGLPIALLEAMAYSLPAVVSDIPANLEVKLPPESYFEVGNVDALAQKIAAL
                     VSSQRIDYSAWLKNYDWQVIARKTASVYHSLANKKG"
     gene            complement(4099..5310)
                     /locus_tag="ON345_002463"
     CDS             complement(4099..5310)
                     /locus_tag="ON345_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3072513.1"
                     /translation="MKILLVNKFFFIKGGAETVYFQERNWLKAAGVDVVDFSMLHEKN
                     FPSEYADTFVRNVDYHKESTLLGEVKTASNFIHNAEACRKMRTLLQRERPDIVHFHNI
                     YHQLTPALIKVARNFGCKTVLTAHDYKIACPAYSMLRDGKVCDACLTGTVFNAFRYRC
                     QEGSATKSLLLSLEATWQSIARNYHMLDVIISPSEFLKGILRRKLPHSRIDVIVNGVD
                     DDPATDKTADKGYLLYVGRLSREKGVATLPLAHQKMRNRAPLKVVGHGPLYDELVANY
                     PDVEFLGYVQQGEALNTLIKEARAVILPSECYENCSMSVLEAMSFAKPVIGSRIGGIP
                     EQIRDGIDGVLFEPGNVQDLANAMDYMIDSPEKARVMGLSARERLREKYTLQKHMETL
                     TALYKEILSWS"
     gene            complement(5324..6313)
                     /locus_tag="ON345_002464"
     CDS             complement(5324..6313)
                     /locus_tag="ON345_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3072514.1"
                     /translation="MNNKPFISVSIKTLNESECIEKTIDSIRQQLRGYPHKIIVADSL
                     STDNTQQLAGDKGVMVVSLTEPGDRCCGVGHQLGYLYSEGDYILLMDGDMELEPGFID
                     RAVTFLEANSEYAGVAGTVEMDEAANYEFISRKQRLDTIYPVGDCDHLGGGGLYRRSA
                     IRKIGYLTNRNLHAYEEAELGLRLLEHGYKLHRLNIPYFRHTSYTLPTFKMLRYRWRS
                     GYYQGMGEILRSAWGKPYFSTVVKMVKSEVVFLLYLMLLVCSVFTLNMDIVGVALLPL
                     LVFIVLKTIKNRSLVNGLYSAMNMTIRAAGLLKGLMQPMRDPIVPPGNKIIHR"
     gene            complement(6303..7688)
                     /locus_tag="ON345_002465"
     CDS             complement(6303..7688)
                     /locus_tag="ON345_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCR3072515.1"
                     /translation="MTLNTGFYLQIYTFITLILCGLVQYFTGMQAVLWLPFCLALVMV
                     GLLVMQTRDGTQGLDDQETIILAVYCSFLALVGLSTLIQGGPVVAIIAFKNEIGLSLV
                     MICLLLGFCRESQIYRVTRCLYWVFYAQIPVMFYQVFFVVPQRVALLGEDEKWDSVVG
                     TFGGDPMGGGNTAAMGLFCLLIMLLKLSEYKHGLTTIKSLVLHIILGLGLCIIGEVKF
                     VILLSPLFLAWVWVTPGYVKDISKVNLKALLVIILGMVLLITLAIVILASFYSAAFGT
                     DPTQSSLSVFWESLSYIFDPNYILSTGELGRFTTLFFWWENNDLLGISGTLFGYGLNA
                     TNSGSSVSPGFLNLIYNLILDSTSLSMLLWEVGVIGTVLFIGLFIFILKVTQAKPVLR
                     LEQLDQQDLQLVSFAPAYNAFVISCLLSIPYSQILMITPMLQFLFYLSLGCSLVIRRT
                     VLRSVESLYEQ"
     gene            complement(7685..8896)
                     /locus_tag="ON345_002466"
     CDS             complement(7685..8896)
                     /locus_tag="ON345_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCR3072516.1"
                     /translation="MSLLKSASTIAGSSVISQLIGAFSIWLISYKYDLAEVGLYALNY
                     SIAVIGAQVCTFASQLLIPKQSEEELTQNVVFCLLQSAILALPYALLTAWLFHQNVLF
                     LYLLSLSNAWVLISENLSLRTGNFRFLIFQRISVSVVVVLSVVLTNQVQMFYWTWASG
                     MMLLTISCIARSFNFRTVTAQHLSLKSNLVFFRTHFHHISRVGSAEVLAMANNNLPIM
                     LINFWFSTLTAGYFSVVSRFCLSPVTIVGNAVRNTIFSKWSIDFRNNTFNYPEYQRVR
                     FLLMVLGVICTLGVFIFYPIVMHLGFGEDWINSIDTSRYMLPYLFPALAVSPLTVIEL
                     IFGSHRYFLRIQLEQLAIVLIAFVVTPYFYNDYATSVIIFSVLTFIRYAFIYLAMNKR
                     ATLLKNNPVMP"
     gene            complement(8893..11025)
                     /locus_tag="ON345_002467"
     CDS             complement(8893..11025)
                     /locus_tag="ON345_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCR3072517.1"
                     /translation="MKLSIVENGKKREETVDVSRFAKEIKKNACKIVLAGIISGAVAY
                     PLISMLSSKYVSTATVLLKAQADNVSPFPQVEDFDSTRTGYYETQYALMQSRIVLEKA
                     VRELKLDQNPDFIGKKADEKASNSEDAEQQRIERALNTLQKNLTVSGIRTTNLATVSY
                     ESTSPQLSSEIANGVAQAFIDYTLDQKRLKTEKAREVNLQKMEEVQKEIAQQKADIDN
                     FLAKEGLLTFRGIDGFETEQLSIVTNRLADATQRRIAAESLEKAVSAGGRVSLDNIIS
                     LPTISNHAQIQDLRIAMIQAQRSLYELQKSYGPKHAKILEAQAQVKAIQDQMGVVLSE
                     LKKGIHQQYLAALADEKDYQAQLDQQKEIFQKLAEKRSLYNSQKLSLDKLEDLYKTLY
                     QRTQELSLSGINADAVLYDPAVPAVKPSKPNKALLLVMVVALAMAFFFMYVIVKAAMD
                     NSIRTLGQVTKRLGVVSLGEIRRIAGAGDRAQVRDLITRNPLNADIIHSIRTQILLDN
                     RPQQVLAISSAKQGEGRSLLASLLANSFSFDQKTLLLDLDFFNRDGLSAEFSTSTSAG
                     VAELLRGEVTLDAARITLSDTLDFLPRGKANASSLLMLSSERFEPLIRDLRNRYQRII
                     VDVSAVSQSQDIELISRVVDGVVFVVQAGAASVETLRAALAKVDANQEVVMGAVLNLV
                     EEKNLQTKESLRSLNITTDELMNTTGRL"
     gene            complement(11028..11585)
                     /locus_tag="ON345_002468"
     CDS             complement(11028..11585)
                     /locus_tag="ON345_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR3072518.1"
                     /translation="MKVINLCTFLLCSILLVGCSSTRPPLTDDASTVTESYILGAGDT
                     VNIMVYGEPEMTMKFIIDKSGAITFPYIGKLMLKGKTAEQVGKEITKKLRGHYLQNPM
                     VTVSIAEFRKFYVSGEVRNPNGYAYEPGMTVEKSIALAGGFTDRADRKDINIRLSGNN
                     ELLENVALTHSVRPGDVVIIGMGFF"
     gene            complement(11587..12873)
                     /locus_tag="ON345_002469"
     CDS             complement(11587..12873)
                     /locus_tag="ON345_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HCR3072519.1"
                     /translation="MRIKLLAITGIVILQPVWAEPIPKSHTGIAGVDFQSDVAANYGY
                     SDNITFQPHSSKEKDSVFQSITPTLSMVGERFQDKYLLMYSGDYRRYDKDSADNYNDH
                     FFRFNGAWRYGLKHGLTLNLEDSLGHEERGRGITEGFVQEQFRQFGITSPLSPHFVNS
                     ELRYSYGAPEGRGKAELALQYKKLRFGKTGGVRNADVDFYNYLLEQEWYENSLIAEVY
                     DQYTANTRFRYSFITNQRRYDRLSEKDSNEYYLRYGVKSQLSDKTNIDMNVAWLYKTF
                     ENNANARNFNGLNWDIQGEWKPLKQSVVTLHTSQNIKDPSEVGGYILFTKYGVSYQHF
                     WLGDRFSTTLDYSLSREDYKKQDKNRRDRNGVFTMKMSYDYTPSVNVELKYLLNKLDS
                     NKNTDSFYIGPNDEREVTRTLGYDNSMIMLTAKVQI"
     gene            complement(12863..14278)
                     /locus_tag="ON345_002470"
     CDS             complement(12863..14278)
                     /locus_tag="ON345_002470"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR3072520.1"
                     /translation="MSTCSLKFNSEECSAFIKVLDFTVINATLITSAWFFETKYFGTA
                     VLFCLLFSTLFLLFSEYTKVYQRKIRKFRFRDFKGILGSALLAILLCEVIRYTLAQVY
                     PQGDFNSLGDDFVSPIVVWYLLPFPALLCIRYLLLKYTSRKSTRVAIVGVTENGLAVE
                     EALRNEYSNMQLDLAFYDERDFSRLDDVAKKIKSPFKGSVQTLVEEARRGNVDEIYIA
                     LPMVALQRIRHFLSMMSDTTVDTYIVPDFYTYSNNMSKFRNIHDLHTIAIFSSPFEGV
                     SSFIKRAEDLIVGSIIMVMISLLMLIIAIGIKLTSRGPVFFKQDRYGLSGQKIKVWKF
                     RTMKVMENDDVVKQATKNDPRVTPFGAFLRRTSLDELPQFINVIQGSMSIVGPRPHAV
                     AHNELYRKQVENYMIRHKVKPGITGLAQVNGFRGEIDTLYKMEKRVQYDIEYIKKWSL
                     WLDIKIIIKTIFKGFVGKNAY"
BASE COUNT         4003 a         3705 c         3178 g         3651 t
ORIGIN      
        1 gataattaag gatattgctg tggcaaaaac gcattaagca gaaacaccga gttattagca
       61 ttttatttat acggatattt tatgacaatt ctcgtcactg gcggtgcagg atatataggt
      121 tcgcatactg tgttggctct ccaggaaagg aatgatgacg ttgtagttct tgataatctt
      181 tctaacgctt cgcaaacctc tctggaaaga gttgcagaat taaccgggaa gaaacccatt
      241 ttctataaag gcgacgtgat ggacagacat cttctgaaga agatttttgc gtcgcataat
      301 attactgatg tgattcattt tgcaggtcta aaatccgttt ctgaatcgat aaaagatccg
      361 ctctcttatt accagaataa cgtcactggc acactggttc tgctcgagga aatgcgtgcg
      421 gccggcgtaa acagctttat attcagttct tcggccaccg tgtacggcaa cccggatcgc
      481 gtaccgctca atgaaaactc ccgtacgggc ggtaccacta atccatatgg cacatccaaa
      541 tatatggttg aacagattct ggaagatttt tcgcgagctc agcctgaatt ccgtattacc
      601 tgtctgcgtt acttcaaccc agtcggggcg catccgtcag gacgtattgg tgaagatccc
      661 aatgggatcc cgaacaatct ggtcccttat attgctcagg tgaccattgg caggctggaa
      721 gtactatccg tgtacggtaa tgattacccc actccggacg gtacgggcgt acgcgactat
      781 attcatgtaa tggatctcgc ccatggccat ctggcagcac tggacaataa ggataaaggt
      841 gacgcgtata aagtctttaa tcttggcacc ggtatcggct attccgtact cgaccttgtg
      901 aacgcgttcg aaaaagcggc gcagacaaaa atcaattacc gctttgcacc gaggagagga
      961 ggagatattg cagaatgctg gtcagatccg tcccgagcac ggcgggaatt gggctggcag
     1021 gccacccgca ctattgaaga aatgatgcgt gatacctgga actggcagaa aaataacccg
     1081 aacggctatc gcagcgtctg atcctcagtt tagatcctct tcacgatggg ccataaacgc
     1141 ccatcgtttc attgcgtcga tgacagaaca gtcacctttg aaatccggta ccagttgctg
     1201 ctttgctgcc cctcattagc cagccggatc cttggcgccc ccgatttatc gacaatcgcc
     1261 acctctataa agtaactgcc tcccggcatt gtccgcggca tatccagcag aaaatccacc
     1321 gtatactcac cgggcaacca gcggaggaga tcttccttcg attctccgct ggctacaggt
     1381 ttcccggaag catccacgag gcggaatgac agcgtataag gaagataaat cggcgcgacg
     1441 ccttcgttta cccaacggct gttgagcgtc agggcggtgc ctggctgccc tactgactca
     1501 tgacttaacg ataccagacg aaaacggtag ccgattttag tcagcgcatt atcgacaatc
     1561 tgccggtact caccggggat aacacgtgat ttcagattaa gcgtgctggc atggtgtgcc
     1621 agtgcccaat caaatatcct ctgtacttcc tcgcgcgtat agtgctgatc gcgttgccac
     1681 tgcgccatat acccgcatat ttccagattc accggtgcgc gtttccatgc atcattaaag
     1741 cctggatagg ctcgctgcgc tgcctcgagc cgctgagggt aatcatcggc catatgattc
     1801 cacgttgacg aaaacatgcg taagtcgccc cagcagtccg cacgccatcc ggcgccctta
     1861 cgcacagcat tcaccaggct attaccgccg cttatcaaca tgactttggg cgtctttgga
     1921 aacgctgaaa aatgcatatt gacgaaccga tcgagctcct ccgtcgtgtt tctttccaac
     1981 agaggtttca gttcagggaa gttgctgtta tgccactctc cccatgatcc caccatacca
     2041 atatccacaa acgccagatt gtcattgcca tcgtagcgtt gacctaatgc ctggagcagt
     2101 cgttgactat aggtaataaa aagcgggtcg gctaagtcag gaatgaagct cttacgatcc
     2161 gcagtccatg tacctttgat gccttttttt atcagccagt cggggatcct ggagccgcta
     2221 cccggctcgg ccagtgccat aaatcgcaga ccaacattca tggccggccg atgtgccgct
     2281 gcgtacttaa aagcgtcatc cacccgcgca aaatgatact tgccttcctc gggctccaga
     2341 tcgcgccagt aaaaacgttc gtactcaatg ccggtatcgg ggtattccgt cagcggcagt
     2401 ttttcaccat aaccctgatg aaaactggcc acgcccatgc ccggattggt taaaggcccc
     2461 gtcagcgcgg ttggcattac cgtcgttatt gccgccatcg acgacagtgg gcacaccagg
     2521 cagaatatgg ccgtcatcca ctgatgtttc atcactctct caaacagatc tttgctactg
     2581 attttttagg cttaaccacc agtcccagat accaacatga taatcatgga acacatcaat
     2641 gcctaaagcg gatttgatca tatacagcgt cagcaggaca caaaacgtta gaaacagaag
     2701 cccagataac agcagaaaag ccgccagcag actggacaac gtcgactcat ggcggaatcg
     2761 cttacgcata tccctgccca tcagaaatac cagataaaaa cgcttgccga aaaacggaaa
     2821 gcttcggcga aaatccaccc tatgccttga ggccagcgtg atcgcaatga tcgccttttc
     2881 aacctcggct ttctgctgcg cagttaattg ctcagcagtt tgctcgtcaa gcgcttcata
     2941 gaaccggtca atgacaagag gacgttgttc agaacgggtc aaaattaacc ttttttattt
     3001 gctaaggaat ggtagacact ggcggttttt ctcgcgatca cctgccagtc gtaatttttc
     3061 agccaggcgc tgtagtcaat ccgctgtgag gaaaccaacg ctgctatttt ttgcgccaga
     3121 gcgtcgacgt tgccgacctc gaaatacgat tctggcggca attttacttc aagattcgca
     3181 ggaatatcac tgaccacggc gggcagtgaa taggccatcg cttcgagaag cgctatcggt
     3241 aacccttcat ggtatgaagg catcaaaaac agccgcgcct gagaaaagat agcctgcaat
     3301 tcctcacctt tgaggaaccc cgtcatgatg acgttcggcg tatctgcagc catcttttta
     3361 aggcggacgc tatattccgt gggatgatcg gcatcaccca ccaataccag cggcatcgtg
     3421 agccccagtt tgcggtgggc agcaatcgca tcatgcatac ctttttcttc cacaaaccgc
     3481 ccaacgacaa ccaggtaatt ttgcggctgc agcgcgtaac gtcccaacac cgtgcgcaca
     3541 gtctcttcct ttaacggcaa cggtaaattg acgccgttgt agatcaagtg tgcatcatca
     3601 cgacagtgtt ttgtgcgtat cagttgatta atcacctctg aaataacgat cacttcattg
     3661 gcatacttaa cagccacttt ctctcccagt tgcagcaccc ttttagccag acgcccccat
     3721 ttctggcgat cgtaatctgg accatggtgg gtaaacacca ccttctttcc tagcacacgc
     3781 agcagtggca ccagaagtcc gggtccgatg gcatgaacgt gcacaatatc agaaccatcg
     3841 aaacaggttc ttaacgtggc taagaaggaa tggacaatcg cttccagcga tcgcttcttc
     3901 ggagcccata gagagtatgt ttcaacattt ttataatacg tctgtttata gctgacgtag
     3961 ggagagcgag cgataacgca gatatccata tcaaactgct tttttatagc cgggtaaaga
     4021 ttctggcagt gtgtttccac accaccctgg acatccggta tcccgcgtgt accgagaacc
     4081 gtgatttttt gtgacatatc aggaccagct cagaatttcc ttatacaacg cagttagcgt
     4141 ttccatatgc ttttgaagcg tatatttttc gcgcagacgt tcgcgagcgg atagccccat
     4201 gacgcgggcc ttttccgggg aatcaatcat gtaatccatt gcattggcga gatcctgcac
     4261 attcccgggt tcaaacagaa ctccgtcgat accgtctcga atttgttccg gaataccccc
     4321 aatccgcgaa ccaatgaccg gtttagcgaa ggacattgcc tccagcaccg acatggagca
     4381 gttctcatag cattcggaag gaaggatcac cgcgcgcgcc tctttaatca gcgtattaag
     4441 cgcctccccc tgctgtacgt agcctaaaaa ttcgacatcc gggtagttgg ccaccagctc
     4501 gtcgtagagt ggaccatggc ccaccacctt taacggcgca cggttgcgca ttttctgatg
     4561 tgccagaggc aatgtggcga cccctttttc acggctgagc cggccaacat acagcaggta
     4621 gcctttatcg gcggtcttgt ctgtcgcagg gtcgtcatcg acaccgttga cgatcacatc
     4681 aatccgcgaa tgcggcaatt tgcgccttaa tatccctttc aggaattcac tcggcgaaat
     4741 tatcacatcg agcatgtgat aatttctggc aatcgactgc caggtcgctt ccaaagaaag
     4801 cagtaaactc ttcgtcgcag aaccttcctg gcagcggtaa cgaaaagcgt tgaataccgt
     4861 cccggtcagg catgcatcgc acaccttacc gtccctcagc atggagtagg ccggacaggc
     4921 gattttatag tcgtgagcgg tgagaaccgt tttacaacca aaattgcggg ccaccttaat
     4981 gagggccggg gttaattgat gatagatatt atgaaagtgc acaatgtctg ggcgttcacg
     5041 ctgcaataat gtccgcattt ttcggcaagc ctctgcattg tgaataaaat tactcgccgt
     5101 tttcacctcg cccagaaggg tgctttcttt gtggtaatca acattacgga caaaagtatc
     5161 ggcatactcg gacggaaagt ttttctcatg cagcatggag aaatccacca cgtcgacacc
     5221 cgctgctttc agccagtttc tctcctgaaa ataaacggtt tccgcacctc ctttaataaa
     5281 gaaaaacttg ttaactagta atattttcat ttcgtcaact ccattaacga tgaatgattt
     5341 tattgccagg cggtacaatc ggatcgcgca tcggttgcat cagccctttc aataatccag
     5401 cagcgcgaat ggtcatattc atagcgctgt atagtccatt aaccagcgaa cgattcttaa
     5461 tagttttaag tacgataaat acaagtagcg gcagcagcgc aacgccgaca atatccatat
     5521 tcagcgtaaa tactgaacat accaacagca tcaaatacag taaaaaaacc acttcgcttt
     5581 ttaccatttt cactacggtt gaaaaatacg gttttcccca ggcgctacgt aatatttcgc
     5641 ccataccctg gtaataacca ctccgccagc ggtagcgcaa cattttgaat gttggcaacg
     5701 tgtacgacgt atggcggaaa taaggaatat tcaggcgatg caatttatag ccatgttcca
     5761 ataaacgaag ccccagttca gcctcttcat acgcgtgtag attgcggttg gtcagataac
     5821 caatttttct gattgcagaa cgacggtata acccgccgcc acctaaatgg tcgcaatccc
     5881 caactggata gatcgtatca aggcgctgtt tacgggagat aaactcatag tttgcggcct
     5941 catccatctc caccgtccca gccacgccag catactctga atttgcttcc agaaaggtca
     6001 ccgcgcgatc aataaagccg ggttcaagct ccatatcacc gtccatcagc agaatgtaat
     6061 ccccttcgct gtagagataa ccgagctgat gtccaacgcc gcaacagcga tcgcccggct
     6121 cggtgagcga aaccaccatt acccctttgt cacccgccag ttgttgggtg ttatccgtgg
     6181 acaggctatc ggcaacgata attttatgtg gataacctct aagctgctga cggatgctat
     6241 cgatagtttt ctcaatacac tcagattcat tgagcgtttt tatgctcact gaaataaacg
     6301 gtttattgtt catacaatga ctcaacacta cgaagaacgg ttcgacgaat taccagactg
     6361 catcccagag ataaataaaa gagaaactgc aacatcggtg taatcatgag tatctggcta
     6421 taggggatac tcagtaagca gctaataaca aaagcgttgt aggcaggtgc gaaactcacc
     6481 aattgcaaat cctgctgatc taattgctca aggcgtaaca cgggtttggc ctgagtgact
     6541 tttagaataa agatgaataa accaataaat aataccgtcc cgatgacgcc aacttcccac
     6601 agcaacatac tcagagaggt ggagtccaga attaaattat aaataagatt caaaaagccc
     6661 ggagaaacag aactgccgct gttggtagca ttcagtccgt aaccaaataa cgtaccggaa
     6721 atacctagca gatcattatt ctcccaccag aaaaagaggg tagtaaagcg ccctagctcc
     6781 ccggtcgaca atatgtaatt cgggtcaaaa atatagctca acgattccca gaagacgctt
     6841 aacgagctct gagtcggatc ggtaccaaag gcagccgaat agaacgatgc gagaataaca
     6901 atcgccaatg taatcagcag caccataccc aggataatca ccagtagcgc tttcaggttt
     6961 accttactga tatctttaac atagccgggc gtgacccaca cccatgccag aaacagaggt
     7021 gaaagtagga tgacgaattt cacttcgcca ataatacaca accctaaacc aagaataata
     7081 tgcagaacca aagacttaat tgtcgttaac ccatgcttat attcagaaag ctttagcagc
     7141 ataattaaca ggcaaaataa gcccatcgct gcggtatttc cgccgcccat cggatcaccg
     7201 ccaaaggtgc ctacgaccga gtcccacttt tcatcttcac cgagcagggc cacacgctga
     7261 ggcacgacaa aaaatacctg atagaacatc accggtatct gggcgtaaaa cacccaatat
     7321 aaacaccgag tgacgcggta gatttgcgac tcgcggcaaa aacccaacag caggcagatc
     7381 atgaccagcg acaggcctat ctcgttttta aacgcgatta tcgccaccac tggcccgccc
     7441 tggatcaagg tcgaaaggcc gaccaacgcc aggaaagaac aatagaccgc gagaatgatg
     7501 gtttcctgat catccaaccc ctgagtgccg tcacgcgttt gcatgaccag caggcccacc
     7561 atcacaaggg cgagacaaaa tggcagccag agaacggcct gcatgccggt aaaatattgc
     7621 actagcccgc acaatatcag ggttatgaac gtgtaaatct ggagataaaa tccggtattt
     7681 aaggtcatgg catcaccggg ttgttcttca aaagcgtcgc acgtttattc atcgccagat
     7741 aaatgaatgc ataacgaata aacgtcagaa cggagaaaat aatcaccgag gtggcatagt
     7801 cgttatagaa ataaggcgtc actacaaaag cgatcagtac gattgccagt tgttccagct
     7861 gaatacgcag gaaatatcgg tgtgatccaa aaatcagttc gatgacggtg agaggactga
     7921 ccgccagcgc cgggaataga tagggcagca tataacgcga ggtatcaatg gaattgatcc
     7981 agtcctcacc gaaaccaaga tgcatcacaa taggataaaa aataaacacc cccagggtac
     8041 atatcacccc gagcaccatc agcaggaaac gaacccgctg gtattccgga tagttaaacg
     8101 tgttatttct gaagtcgatc gaccactttg agaaaatggt attacgcacc gcattcccga
     8161 caatggtcac cggtgacaga cagaagcgac tgaccacgga aaaatagccc gccgttaatg
     8221 tcgaaaacca aaagtttatg agcatgatcg gcagattatt gttagccatc gccaatactt
     8281 cggcactccc gactcgcgaa atatggtgaa aatgcgtcct gaaaaacacc aggttgcttt
     8341 tcagggacag atgctgcgcc gtgaccgtac ggaaattaaa agatcgcgcg atacaactga
     8401 tggttagcag catcatgccg ctggcccagg tccagtaaaa catctgaacc tgattcgtca
     8461 gtacaacaga cagcaccacg acgactgaca ccgaaatacg ctggaagatg agaaaccgga
     8521 aattacccgt gcgcagtgac aggttttccg atatcagaac ccatgcgttc gacagcgata
     8581 acagatagag aaacagcacg ttttgatgga atagccacgc cgtcagtagc gcgtaaggca
     8641 gcgccagaat cgcgctttgc agaaggcaaa acaccacatt ttgcgttaac tcttcttccg
     8701 attgtttagg aataagcaat tgggacgcaa aggtacatac ctgcgctccg atcacagcga
     8761 tactgtaatt gagcgcatac aggcccactt ccgcgagatc gtatttatac gagattaacc
     8821 agattgagaa ggcgccaatc agttgcgaaa tgactgacga cccggcaata gtggaagcac
     8881 tcttcaataa actcataacc gacctgtggt attcatcaat tcgtcagtag tgatattgag
     8941 cgagcgaaga ctctctttcg tctgcagatt tttttcctca accagattga gtaccgctcc
     9001 catgaccact tcctggttgg cgtcaacttt cgccagcgcc gcgcgcagcg tctccacgga
     9061 cgcagccccc gcttgcacaa cgaaaaccac accatcaacc acccgactaa tcagctcgat
     9121 gtcctgactc tggctcaccg cagagacatc gacgatgatc cgctggtagc gatttcgcag
     9181 gtcacgaatg agaggttcaa aacgttccga agacagcatc agcaaagacg aagcgttcgc
     9241 ttttccgcgg ggtaaaaagt ccagcgtgtc actaagcgtg atccgcgcag cgtcaagtgt
     9301 cacttctcca cgcaacagct ctgcaactcc cgcagaggtc gatgttgaaa actcggcgga
     9361 caggccatca cggttaaaga aatccaaatc aagcagtaag gttttctgat caaagctgaa
     9421 ggagtttgcc agcagactgg ccagtaaaga gcgcccctca ccctgctttg cagaggagat
     9481 tgccagaacc tgctgcgggc ggttatccaa caaaatctgt gtacgaatgc tgtggataat
     9541 gtcggcgttc aaggggtttc gcgtgatcaa atcgcgaacc tgtgcacggt ccccggcccc
     9601 cgcaatgcgg cggatctcac ccagtgagac gacgcccagt cgttttgtca cttgtccgag
     9661 cgtcctgatg gaattatcca tcgccgcttt tacaatgacg tacataaaga agaaggccat
     9721 ggccagcgcc accaccatca ctaacagtaa cgctttattt ggcttagatg gcttcactgc
     9781 cgggacagcc ggatcgtaca gcactgcatc cgcattaatg ccggacagag acagttcctg
     9841 ggtccgctga tacagggttt tataaagatc ttccagttta tccagtgaca atttctggct
     9901 gttatacagg ctgcgttttt cagccagttt ctgaaaaatt tctttctgtt gatcaagttg
     9961 cgcctgataa tccttttcat ccgccagcgc ggccagatat tgctgatgaa tgcctttttt
    10021 aagctcactg agcaccacgc ccatctgatc ctgaatagcc ttcacctgag cctgcgcttc
    10081 caggatcttc gcatgtttcg ggccatatga tttttgtaac tcatacaaag accgctgcgc
    10141 ctgaatcatg gcgatacgca aatcctgaat ttgcgcatgg ttagagatcg tcggtaatga
    10201 aatgatgtta tccagagaga cccggccccc agcgctgacg gctttttcca gagattctgc
    10261 cgcaatacgc cgttgggtag catcggccag acggttggta acaatgctga gttgctcggt
    10321 ttcgaagcca tcaatgccgc ggaacgttaa taagccctct ttcgccagga agttatcgat
    10381 atcggctttc tgctgcgcga tctctttctg cacttcctcc attttctgaa ggtttacttc
    10441 tctggctttt tctgtcttca gccgcttttg gtccaacgta taatcgataa acgcctgtgc
    10501 gacgccgttg gcaatctcag aggagagttg tggcgatgtc gactcataag agactgtcgc
    10561 cagattagtg gttcgaatac cgctaacggt aagatttttt tgcagcgtgt tcagcgcgcg
    10621 ctcaatgcgc tgctgttcag catcttcgct gttgctggcc ttttcatccg cttttttgcc
    10681 aataaagtct gggttttgat ccagctttaa ctcgcgaacc gctttctcca gaacaatacg
    10741 cgactgcatc aaggcatatt gcgtctcata gtagccggtg cgcgtggaat caaaatcttc
    10801 cacctgtggg aacggcgaaa cgttatcagc ctgagccttt agcaacaccg tagctgttga
    10861 gacatatttt gatgacagca tgctgattaa tgggtaggca actgcaccgc tgataattcc
    10921 cgcaagtaca atcttacagg cgttcttttt aatttctttg gcaaatctgg aaacgtcgac
    10981 ggtctcttcc cgtttttttc cattttccac tattgataat ttcatcgtta gaaaaatccc
    11041 attccaatga tcactacgtc cccaggacgg acagaatggg tcaatgccac attctcaagc
    11101 agttcattat tccctgagag tcggatattg atatctttgc gatccgcgcg atcggtaaac
    11161 ccacccgcca gggcaataga cttctcaaca gtcatgccgg gttcataggc ataaccattg
    11221 ggatttctaa cttctccaga gacatagaat ttacgaaatt ctgcgatgct gacggtaacc
    11281 attgggtttt gcaaataatg cccacgtagc ttcttggtaa tttctttccc cacctgctct
    11341 gccgttttcc ctttcagcat cagtttaccg atataaggga aagtaatagc gccgctttta
    11401 tcgatgataa atttcatggt catctcgggt tcaccgtaaa ccatgatatt gaccgtatcg
    11461 ccggcaccca gaatataact ctccgtgaca gtcgacgcgt cgtctgttaa aggcggtcgg
    11521 gtggatgagc aacccaccaa caaaatactg cacagtaaaa atgtgcacaa attgattact
    11581 ttcattttat atctgaacct tagcagtaag cataatcatt gaattgtcat aacctaacgt
    11641 ccttgttact tcccgctcgt cgttaggtcc aatgtagaac gaatccgtat tcttattcga
    11701 atccaactta ttcagaagat acttgagttc aacgttaacc gaaggggtat agtcgtaact
    11761 cattttcatc gtaaacacgc cattcctgtc acgacgattt ttgtcctgtt ttttgtaatc
    11821 ctcccggcta agagaataat ccagggtagt agaaaaacga tcccccaacc aaaagtgttg
    11881 gtacgaaaca ccatatttgg tgaataaaat atatccaccc acctcggacg gatctttaat
    11941 attctgtgaa gtatgtagcg taacaacaga ctgttttagc ggcttccact ctccctgtat
    12001 gtcccaattc aatccattaa aatttcgagc gttggcatta ttctcaaacg tcttatacaa
    12061 ccacgcgaca ttcatgtcga tgtttgtttt gtccgataat tgggacttta ctccatagcg
    12121 caaatagtac tcgttgctat ctttttcaga taaacgatcg tatcgacgct ggttagtaat
    12181 aaaactataa cgaaaacgtg tgttcgcggt atattggtcg taaacttcag caataagact
    12241 attttcgtac cactcctgct caaggagata attgtaaaag tctacatcag cattacggac
    12301 cccgcccgtc ttaccgaaac gtaacttttt atactgtaat gccaactcag ccttgccgcg
    12361 tccttcaggt gcaccataac tgtaacgcaa ttcgctattc acaaaatggg ggctcaaagg
    12421 agacgtaata ccgaactgtc taaattgttc ctggacaaaa ccttctgtaa ttccgcgccc
    12481 gcgctcctca tgccccagag aatcttccag attcagggtc agtccgtgtt tcagcccgta
    12541 tcgccatgcg ccgttaaaac ggaaaaaatg atcgttgtag ttgtcggcag agtccttatc
    12601 gtaacgacgg tagtcaccgg aatacatcag caaatattta tcctgaaaac gctcgcctac
    12661 catacttaac gtcggcgtaa tgctctgaaa tacagaatct ttctccttgc tgctgtgtgg
    12721 ttgaaaggtg atgttatctg aataaccata attcgctgca acgtcgcttt gaaaatctac
    12781 ccccgcaatg cctgtatgtg atttcgggat gggttcggcc cataccggtt gtagaatgac
    12841 aattccggta atggccaaaa gtttaatacg cattctttcc gacaaaccct ttaaaaattg
    12901 ttttaataat aatttttata tccagccata aagaccattt cttgatgtat tcaatgtcgt
    12961 actgaactct tttttccatt ttatagagcg tatcaatttc cccacggaag ccgttgacct
    13021 gcgcaagacc agtgatcccc ggttttacct tatgacgtat catgtagttc tcaacctgct
    13081 tacggtacag ctcgttatgt gcaaccgcat ggggtcgggg gccaacaatt gacatacttc
    13141 cctgaatgac gttgataaat tgcggcagct catcgagaga agtgcggcgt aaaaaggccc
    13201 cgaagggtgt cacccgagga tcgttcttcg tggcttgttt cacgacatca tcgttttcca
    13261 tgaccttcat ggtgcggaat ttccacactt taatcttttg tccgcttagc ccgtaccgat
    13321 cctgtttaaa aaatacaggt ccacgagaag tcagtttgat gccaatcgca ataatcagca
    13381 ttaataagga aatcataacc atgataatgc tgcccacaat cagatcttcc gcacgcttga
    13441 taaatgaact cactccttcg aacggcgaac tgaaaatagc aatggtatgc agatcatgga
    13501 tattgcgaaa tttggacata ttgttactgt aagtataaaa gtcaggaaca atataggtat
    13561 ccaccgtagt atctgacatc atcgacagaa aatgacggat acgctgcaaa gcaaccatcg
    13621 gcagagcgat ataaatttca tcaacattcc ctcgtctggc ctcttcgacc agtgtctgta
    13681 ccgaaccctt aaatgggctt ttaatcttct ttgctacatc atcgagccgc gaaaaatccc
    13741 gctcatcata aaaagcaagg tccagctgca tgtttgagta ttcgtttcgc aatgcctctt
    13801 caactgccag gccgttttcg gtaacaccaa caattgccac ccgggtgctt tttctggacg
    13861 tatacttaag aagtaaatag cgaatacaca aaagagcagg aaacggtaat agataccata
    13921 cgacaatagg ggaaacaaaa tcatcaccca gcgagttaaa atccccctga ggatatactt
    13981 gtgcaagagt atacctgatt acttcacaaa gaagtatagc cagtaatgca ctgccgagaa
    14041 tccctttaaa atcacgaaaa cgaaatttcc tgattttccg ttggtatacc tttgtgtatt
    14101 ctgaaaataa caaaaagaga gtagagaaga gcagacaaaa taatactgcc gtaccaaaat
    14161 acttcgtttc aaagaaccag gcgcttgtaa tcaatgttgc attgattaca gtgaaatcaa
    14221 gcaccttgat gaatgcagaa cattcttcac tattgaattt taatgaacat gtcgacatat
    14281 taaacactac aacaaccttt caccacatgt catttacaat ggtaaaaaac cattaataaa
    14341 attaactgat ggatgggttg ttatttatct cagcgtaatc cctaacatac agtgcattcc
    14401 atgcaccgtc cctattaatc tgcaacgata cattactgtg attattgggt tcaattcgaa
    14461 aaaaaatgag atttcttcag gatattccga atctgagtca ctacaatact gcttctgagt
    14521 cttatgaatg agataaa
//
LOCUS       DAJSKJ010000096        14431 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000096 DAJSKJ010000000
VERSION     DAJSKJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.096"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            90..1199
                     /gene="anmK"
                     /locus_tag="ON345_002471"
     CDS             90..1199
                     /gene="anmK"
                     /locus_tag="ON345_002471"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR3072521.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1297..1626
                     /gene="mliC"
                     /locus_tag="ON345_002472"
     CDS             1297..1626
                     /gene="mliC"
                     /locus_tag="ON345_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR3072522.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1685..2341
                     /gene="pdxH"
                     /locus_tag="ON345_002473"
     CDS             1685..2341
                     /gene="pdxH"
                     /locus_tag="ON345_002473"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR3072523.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2470..3744
                     /gene="tyrS"
                     /locus_tag="ON345_002474"
     CDS             2470..3744
                     /gene="tyrS"
                     /locus_tag="ON345_002474"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR3072524.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3803..4666
                     /gene="pdxY"
                     /locus_tag="ON345_002475"
     CDS             3803..4666
                     /gene="pdxY"
                     /locus_tag="ON345_002475"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR3072525.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(4710..5315)
                     /gene="gstA"
                     /locus_tag="ON345_002476"
     CDS             complement(4710..5315)
                     /gene="gstA"
                     /locus_tag="ON345_002476"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR3072526.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEDYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5421..6923)
                     /gene="dtpA"
                     /locus_tag="ON345_002477"
     CDS             complement(5421..6923)
                     /gene="dtpA"
                     /locus_tag="ON345_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR3072527.1"
                     /translation="MSTAKQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(7535..8170)
                     /gene="nth"
                     /locus_tag="ON345_002478"
     CDS             complement(7535..8170)
                     /gene="nth"
                     /locus_tag="ON345_002478"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR3072528.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(8170..8865)
                     /gene="rsxE"
                     /locus_tag="ON345_002479"
     CDS             complement(8170..8865)
                     /gene="rsxE"
                     /locus_tag="ON345_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR3072529.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(8869..9489)
                     /gene="rsxG"
                     /locus_tag="ON345_002480"
     CDS             complement(8869..9489)
                     /gene="rsxG"
                     /locus_tag="ON345_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR3072530.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAQELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(9493..10551)
                     /gene="rsxD"
                     /locus_tag="ON345_002481"
     CDS             complement(9493..10551)
                     /gene="rsxD"
                     /locus_tag="ON345_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR3072531.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(10552..12486)
                     /gene="rsxC"
                     /locus_tag="ON345_002482"
     CDS             complement(10552..12486)
                     /gene="rsxC"
                     /locus_tag="ON345_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR3072532.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQIVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSNIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            complement(12479..13057)
                     /gene="rsxB"
                     /locus_tag="ON345_002483"
     CDS             complement(12479..13057)
                     /gene="rsxB"
                     /locus_tag="ON345_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR3072533.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(13057..13638)
                     /gene="rsxA"
                     /locus_tag="ON345_002484"
     CDS             complement(13057..13638)
                     /gene="rsxA"
                     /locus_tag="ON345_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR3072534.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(13715..14155)
                     /locus_tag="ON345_002485"
     CDS             complement(13715..14155)
                     /locus_tag="ON345_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR3072535.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(14241..>14431)
                     /gene="ydgT"
                     /locus_tag="ON345_002486"
     CDS             complement(14241..>14431)
                     /gene="ydgT"
                     /locus_tag="ON345_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR3072536.1"
                     /translation="FRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSG
                     GRLFDLGQVPKSVWHYVQ"
BASE COUNT         3643 a         3682 c         3708 g         3398 t
ORIGIN      
        1 caacggtaaa aatcggtcat tccgcatggt attcacgaaa agtttatgtt tgagtggtgc
       61 gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt
      121 caggcaccag ccttgatggt gttgatgttg tgttggcgac aatagatgaa caccgggtcg
      181 cacagctggc aagcttgagc tggccgatcc cggtgtctct gaaacaggct gtactggata
      241 tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact caactcgggc
      301 gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaacctgcag gcgagagata
      361 tagttgcgat cggctgtcac ggtcaaaccg tctggcatga accgacgggt gtggcaccac
      421 acactttaca gattggcgat aacaatcaaa ttgtggcacg caccggaatt acggttgtcg
      481 gtgattttcg ccgtcgcgat attgccttgg gaggacaagg cgcaccgctg gtacctgcgt
      541 tccatcatgc cttgctggct cacccaaccg agcgacgaat ggtgctcaat attggcggca
      601 tcgccaatct gtcactgctc attcctgggc agccggttgg cggctacgat accggtcctg
      661 gtaacatgct gatggatgcc tggatctggc gtcaggccgg taaaccttac gataaagatg
      721 ccgagtgggc acgggcgggt aaagttattc tcccactgct gcaaaatatg ctcagcgacc
      781 catatttctc gcaacctgca ccgaaaagca ccggacgcga atactttaac tacggctggc
      841 tggagcgcca tttacgccat tttccgggtg ttgatccccg tgatgttcag gccacactgg
      901 cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc tgcgaacgat
      961 tgatggtatg tggtggaggt agtcgtaatc cgctactcat ggcgcgtctg gcggcattac
     1021 tgccaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat gacatggaag
     1081 cattggcttt cgcctggctt gcctggcgga cgctggcggg attaccagga aatctgcctt
     1141 ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccctgct aacccgtgat
     1201 atcagagtta actgaatttt tcttaccgcg tctgccgtta cacttggaac gttagggagg
     1261 gcgtattgcc ctccagacca ggaaagtctt cgggaaatgt ctatgaaaaa actgttactg
     1321 atctgtttgc cggtgcttct caccggctgt agcgcgttca atcaactggt tgagcacatg
     1381 caaaccgcta cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
     1441 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcatt
     1501 tcagcgtctg gtgcacgtta cactgacgga atctatgttt tctggtcgaa aggcgatgaa
     1561 gcgactgtct ataaacgcga ccgcatcgta ttgaataact gtcagttaca aaatccacag
     1621 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
     1681 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
     1741 aggcgggtta cgccgtcgcg atcttcccgc cgatccatta accctttttg aacgttggct
     1801 ctctcaggcg tgtgaagcca aactggcaga ccctaccgcg atggtggtcg ctaccgtgga
     1861 tgaacatgct cagccttatc aacgcatcgt tttactcaaa cattacgacg aaaaaggcat
     1921 ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata atccgcgcgt
     1981 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcaga
     2041 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
     2101 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
     2161 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
     2221 cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
     2281 tgaccgcttt ttgtaccagc gagaaaatga tgcgtggaag attgatcgtc ttgcaccctg
     2341 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
     2401 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
     2461 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
     2521 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
     2581 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
     2641 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
     2701 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
     2761 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
     2821 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
     2881 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
     2941 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
     3001 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg gtgtggtgct gcaaattggt
     3061 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
     3121 aatcaggtgt ttggcctgac cgttccgctg atcactaaag cagatggcac caaatttggt
     3181 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
     3241 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
     3301 atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg taaagcaccg
     3361 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
     3421 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
     3481 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
     3541 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
     3601 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
     3661 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
     3721 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
     3781 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
     3841 ttatggtcat gcgggtaaca gtgcggcaga gtttcctatg cgccgcctgg gcgcgaacgt
     3901 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
     3961 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
     4021 acacacctgt gatgccgtgt taagtggcta tctgggatcg gcggagcagg gtgaacatat
     4081 cctcggtatc gtccgtcagg tgaaagccgc gaatccgcag gcgaaatatt tttgcgatcc
     4141 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
     4201 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
     4261 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattgc
     4321 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
     4381 ttttgaaatg ctgctggtca ccgccgatga agcctggcat atcagccgtc cgctggtgga
     4441 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
     4501 actgcttcag ggggcaacgc tgcaggaggc gctggaacat gtgaccgctg cagtctacga
     4561 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
     4621 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
     4681 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
     4741 tgtacttccg gacgttcagc catacgttgc ataaatgctg caatgtgctc taacccttcc
     4801 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
     4861 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
     4921 ttcttctcca gctgcgcgcg aactgtcggt ttgtaatctt ccggtgtatc agggcgaaac
     4981 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
     5041 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
     5101 tactgcataa tcgctacgcc ttccgtcagc aaagtaccgt catccagcag caatgcaggc
     5161 acctgcccct tagggttaac ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
     5221 aaatccacac tgacgagggt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
     5281 agagagcagg cacccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
     5341 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcgacttg
     5401 taaaagagtt tccctgaatt ttacgctaca gcagctttcg ccgctttgtc tgcagcgtca
     5461 tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca tcagtactgc aataatggca
     5521 gtagcgacac caatctgcaa gaatacgcga ccataaactt ccagtgacat cagcgggtcg
     5581 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
     5641 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
     5701 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
     5761 cttgcgacca gccagcttac agacacgata ccagcgtcag acgcgaattt cgcacccagc
     5821 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
     5881 ggcagggtat cgcccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
     5941 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
     6001 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
     6061 aagataatgg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
     6121 ttcatcgcga aggcttcttt accgaagata accacgatac cgaaggcaac aacgcccagc
     6181 gccatacgcg caacttcctg attgtgcagc agccaggtgg cgatagcgat cagtgccaca
     6241 acaccaataa tggtcagcag caggttacgg tagttgatag gctcgaagtc tggttttgaa
     6301 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
     6361 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggcgtagca
     6421 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
     6481 agacgcgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
     6541 aggccgttac cgaccgcaat agccgccata cccatataaa cgataccggc gtcgtgacca
     6601 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
     6661 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
     6721 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
     6781 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
     6841 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
     6901 ggtttttgct ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
     6961 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
     7021 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
     7081 aactgaggtg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
     7141 aacgtaacgg cagatttttt cactgcactg agaggggggg tgatggctta acggcgatat
     7201 gttctgctat ttagcgtttt tactagtgag atagtccagt ttctgaaaaa tagccagtgt
     7261 aatgttttgt aggtcaatat ttgttgcgtg tgtaaattta aatgatttta acataattct
     7321 tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat agaaaatttc
     7381 gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt acctgatgat
     7441 aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga taaagaaagg
     7501 ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc tctttgtatt
     7561 cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg caggtataac
     7621 gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga accactttca
     7681 gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta cgattacaaa
     7741 cgcggaaaat gtgtgtgtcg acagcaatag tcggccagcc gaatgcagtg tttaatacga
     7801 cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga tcttccggaa
     7861 cctcgccatt atgctgctcc agcaagatac ggcaggtttt gatgatattt tctgctttgc
     7921 tgttataaag cccaatcgtt ttgatatagg ttttcacccc ttcaacgccc agttcaagca
     7981 tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta acactgacat
     8041 cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa ctgaaattaa
     8101 gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc aggcgttttg
     8161 ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg cagctgcttc
     8221 agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca tcagtcccag
     8281 gccaataaat gcacctggag gcagcatcgc cagcaggaaa ggggagtcgg tgtggaaaat
     8341 ttccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt caaacaatgt
     8401 gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg ttgcgcccat
     8461 accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg cgaaggcttc
     8521 agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc ctaatgattg
     8581 atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca ccgaggcgat
     8641 gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca gcgtcgaaat
     8701 ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca gagcgttagt
     8761 ggcagtggac gtgaccgcca acagaggaca aaggccgagc aactggacca gcgcagagtt
     8821 gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt attctccaca
     8881 ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg cgcgttttac
     8941 cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga aatcaccacc
     9001 atctttcttc accgcccagt gcgcatcatc tgcaccactg atttttttac ccgcaaaatg
     9061 ggtgatccag tcagaaaggc gcagttcgat tttatcgcca agccctggcg tttcgtggtg
     9121 ctctgtcacg cgcgtgccaa gtaccgtgcc gttaaaatcg gctcccacca gcagctgaat
     9181 cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg gtttgtcatc
     9241 ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tcttgcgcag ttaccagata
     9301 gcaactctgt gccagcgcat tgttatagcg ttcggctggc agcacctgat caaataacgc
     9361 cttttgttgc agactggcct gttcagcaat cgtcgttttg gtcatctggt tgatggccgc
     9421 agttaaccct gttgaacccg ctgcaaatag cgccaacgta atgccgtgtt ttcggatagt
     9481 tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt agtaatcgat
     9541 cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag gatagccgcc
     9601 gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaaaataa gacgaccacg
     9661 attggtcgta gaagcggtaa ccgggtcagt caaaataaag aatgcgccga gcatggtcgc
     9721 accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca accagcccaa
     9781 cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc gaatcgcttt
     9841 ctgccatagc aaccacaggc cgccagccag ccaggcgaga tttacccatt gccaaccagc
     9901 gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg aatgaccggc
     9961 acggacagag gttttaaatg tatccagcgg tgtcgcctga ctaatgccat caatacctaa
    10021 gcgtagtgtg ttcatatcac caccactggc ggtatgtccg ctgaaaataa cctgtatggc
    10081 gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc tggtcatctg
    10141 caccgggaag gagatcagta agaccacata accaatcatt gccggattaa acgggttttg
    10201 ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac ccagcacgac
    10261 catccaccat ggcgcgaggg ggggaatact taccgccagc aataagcctg tcagcaatgc
    10321 tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta cgagagcttc
    10381 ggctaacaga gcactaaccg atgccaacag gatctgaacg agagtacccc aaccaaaaaa
    10441 ccacagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca taatgcgcga
    10501 tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca tttagtcctc
    10561 gtttacaacc ttctgctggg cggctttttt ggcctgagcg cgggcaatag ccgcggcaac
    10621 tgccgctttg cgcggatcaa cctgttcttc tggttctgcg ttagtctgtt gctgttccag
    10681 cttgcgcgct ttggcacggg caatagcggc ttcgacggcg gctttgcgcg gatcgacctg
    10741 ttcttctggt tccgcattag cctgttgctg ttccagcttg cgcgctttgg cgcgggcgat
    10801 agctgcttca acggcagttt tacgtggatc agcaacggtt gctgcgtcgt tagtttgctg
    10861 cagttctgcc tgtttcgctc tggcttgcgc tttacgggct tcccgtgctg caataattgc
    10921 actgttatcc gggcgttcgc ccgctttaat cacaataggc tgtgtagcct gggcctgttt
    10981 ctctttcacc cgcgccagag cggcagcaat cgcatcttta tctttggctg caggttgaac
    11041 ggctgcgctc ttatgtcgtt caaggcgagc cgctttttcg cgctccagac gagcctggcg
    11101 cgcttcgaaa cgcgctttgg cttctgcggc gcgcttttct tcctgacgaa tagccgcaat
    11161 ttcagctttt tcctgacgga aatattgcac caggggaata ttgctcgggc aaacccacgc
    11221 gcaagcccca cattcaatgc aatcagcaat gttatgcgtg gtagctttat cgtgttgctg
    11281 acctttgctg aaccagtaca actgttgcgg caaaagatcc gcagggcagg cgtcagcaca
    11341 ggcgctacac cggatgcagc tttgttcttc ctgtggttcg ccaagttcat tggcagaggg
    11401 agccaacaga cagttggtaa tctttacgac cgggacatcc agccatggca aggtaaagcc
    11461 cattagcggg ccacccataa tcaccatttg atcggcagag gggcagaatc cggcatcatt
    11521 caataaatga cgcactggcg tccccagccg tgcccagacg ttgcccgggc gagcgattgc
    11581 ttcgccagtc agggttacaa cacgctcggt aatcggctcg ccatcaataa cggcacgttt
    11641 cactgcataa gcagtgccga cgttttgcat taatacgccg atattggatg aacgcccgcc
    11701 atgtggaacc tgcttcccgg tcagaatgta ggttaattgt ttagcaccgc cagaaggata
    11761 tttggttgga atcacccgca gagaaatatc gttagagtcc gccagcaccg cgcgcagcat
    11821 ggaaatcgcc tgcggtttgt tatcttcaat gccgataaga atttcgcgtg gctgcagaat
    11881 atgcgcaaga atgcgaatac cctctacgat ctgagccgcg caatcctgca tcaaacggtc
    11941 atcggcggta atgtacggct cgcactcagc cgcgttgata atcaacgttt caatcttatc
    12001 tccgccaccc tgcaatttaa cgcctgtcgg gaatcctgca ccgcccagcc cggcaacacc
    12061 aaactgatgt atgcgctcga ttaactcttc gcgactgcga gtgcgataat cggcccagcc
    12121 gtcgcgcggg atccagcagt cttcaccatc ggcatcaata atcacgctta attcagctaa
    12181 agctgaagga tgagccgtag agtggggcgc aatagccgta acggtacccg aggtgggcgc
    12241 gtgaacaggc agcattttgc cgcgaccacg ggtaagcggc tggccgcgca atactttatc
    12301 gccgacgcta acgcacaact caccttcagc gccaatatgc tgtttcagtg gaataacaaa
    12361 acgctgcgcc agtggtacct ggcgcagggg tgtaccgttg gactgggttt tcatctccgg
    12421 tggatggatg ccgccgttga aatcccagat tttatttttt ctgaatgcag agaataactt
    12481 aagcatggtg ttccacggga atgatacgca cgggaatggt gttcagatcc catttccagg
    12541 agtcaggtgt ttctgcgacc ggttgcaacg agatgcagtg cgtcgggcac ggatcaacac
    12601 ataaattgca gcccgtacag agatcactca ttaccgtatg catggcacgg gtagcgccaa
    12661 cgatggcgtc taccggacaa gcctgaatac atttagtgca gccaatacag ttattttcat
    12721 caataaccgc caccatccgc gcaggcgtta gctcttgcgc ttcgccatcc agcggctgcg
    12781 gctcgacatt aagcaactcg gcaattttta gcatcacagc ttcgccacct ggggcgcaac
    12841 ggttgatttt ttcaccgtta cagctgatgg cttccgcgta ggggcgacag ccgggataac
    12901 cgcactgacc acactggctc tgcggtaaga tttcgtcaat tttctcaacg accggatcgt
    12961 cttccaccgc aaaacggcgg gaggcataac ccagaatggc gccaaacgcc aggcccagca
    13021 ggctcacggc ggcaacggca atccagatag cattcattac aacttcacca aaccactaaa
    13081 gcccataaag gccagagaca taagacctgc ggtaattaac gcaatggcat taccgcgaaa
    13141 aggtgccggg acatcagcca cagcaaggcg ttcgcggatg gcggcgaaga gcaccatcac
    13201 cagcgagaaa ccgacagcgg cggaaaaacc gtacagcgcc gactgcaaga aattgtgccc
    13261 gagattgata ttcagcaacg ccacgccgag cactgcacag ttggtggtga taagcggcaa
    13321 aaaaatcccc agcagacggt aaagcaccgg gctggttttg cgcaccacca tctcggtgaa
    13381 ctgcacgacc acagcaatca ccagaataaa tgccagggtg cgcaggtaaa tcagattaag
    13441 tgggatcaaa atccacgtat cgataagcca ggcgcaaata gacgccagcg tcatcacaaa
    13501 cgttgttgcc agccccatgc ccatcgcggt ttccagtttt ttggaaaccc ccataaacgg
    13561 acagagaccg agaaacttga ccagtacaaa gttattgacc agtacagttc cgacaaacag
    13621 taacaggtag tcagtcattt ttcggcctga aataaaaaag ccgcctatta tccgttaatc
    13681 cagagcaggc gacaacaggt taactgtaag gttattacgg attcacaaac gtcgctttta
    13741 cccgcgacga gcgtttgaaa taggggacga tcaatgcggt tgccaacaaa gtaaacagca
    13801 actggcgaac ggctatgcca tcttccacgg gtgaaaaagc gaatgctttt actgccagca
    13861 atacggaaat aagcagccag ataatatagt gtttaggaac acaacgccga cgtttaaaga
    13921 aagcgatggt caaccacagt gtgtaatacc acatggcgat agcggtaata aaagaaacgc
    13981 cccataagat ttgtgtcgtc agtgcttgtc cgccgagcgt ttgaaatgtt tgcggagaag
    14041 ataacgcagc ggtatacagc aacaacgcca gcgtcgtact caacagagcg actaaaagcc
    14101 aggccaacgg gccaagcaac cagcctccaa tacgttgtgg cgttgtggtg gtcataggtt
    14161 ctccagaata gcgacgcaaa caggcaaatc taattttagg ggcgttatta taaggctttt
    14221 ataaccgatc gctactttct ttattggaca tagtgccaga cggacttcgg tacctggccg
    14281 aggtcaaaca aacgcccgcc agaaaccagc tctgcgcgac ggtgatcggc agcacgatac
    14341 atattgatca gttcctgatc atccgtcagg gtgtaattaa gatggtcgta gagtttttcc
    14401 agactttcga gtgaactgat tttgcgaaat t
//
LOCUS       DAJSKJ010000097        14329 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000097 DAJSKJ010000000
VERSION     DAJSKJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.097"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..173)
                     /locus_tag="ON345_002487"
     CDS             complement(<1..173)
                     /locus_tag="ON345_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR3072537.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(345..500)
                     /locus_tag="ON345_002488"
     CDS             complement(345..500)
                     /locus_tag="ON345_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072538.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(580..642)
                     /locus_tag="ON345_002489"
                     /pseudo
     CDS             complement(580..642)
                     /locus_tag="ON345_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1031..1678)
                     /locus_tag="ON345_002490"
     CDS             complement(1031..1678)
                     /locus_tag="ON345_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072539.1"
                     /translation="MEKDTKRGSALVDALNADQLSDLGKDYGEIAVDSLMDSGLLKEI
                     PVIKTIFAVSGAIGSVRDYLFTEKLIRFLFNFSDLSDAQRINMTEKLNGDDNFAGKAG
                     QRLIEIIDRLESENKPEIAASFLKAFACDFIDFITLRRLMVALERIPAFDIEKLEVFS
                     HCNNDNHPGSEFDKAILLSFGSAGLAENNGGFDGGTILPTNLCSIFVKFKSFKPE"
     gene            complement(1995..2216)
                     /locus_tag="ON345_002491"
     CDS             complement(1995..2216)
                     /locus_tag="ON345_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR3072540.1"
                     /translation="MFHCPKCHFAAHARTSRYFSDTTKERYHQCTNINCSATFVTTET
                     VDRFIVSPGEVVPAPPHPTTSGQQQIHWM"
     gene            complement(2288..3463)
                     /locus_tag="ON345_002492"
     CDS             complement(2288..3463)
                     /locus_tag="ON345_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR3072541.1"
                     /translation="MISGIKIEAGARIAPAFMLTLDGNDITQNFSDRLIGLTMTDNRG
                     FEADQLDIELDDSDGLVELPPRGASLTLWLGWEGSALLNKGSFTVDEIEHRGAPDTLT
                     IRGRSADFRGSLNSRREQSWHDTTLGQVVEAIAQRNKLTASVADTLKAIPVPHADQSQ
                     ESDAVFISRLAERNGATVSIKAGKLLFLKAGSGVTASGKPIPQMTVERGDGDRHSFAI
                     ADREAYTGVTAKWLHTKDPKPQKQKVKLKRKPKEQHLRALQHPKAAKTTAKAKTKKEQ
                     EAREGEYMAGESDNVLELTTIYATKAQAMRAAQAKWDKIQRGVAEFSITLATGRADLF
                     PETPVAVKGFKRVIDEQAWLISRVVHNLNGNGYTTGLELEVKVSDVEYESEELNQEL"
     gene            complement(3460..3924)
                     /locus_tag="ON345_002493"
     CDS             complement(3460..3924)
                     /locus_tag="ON345_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3072542.1"
                     /translation="MLMVLGLFVFERRTLPYQSMQYSKDYRWASNDRIGKTPAYQFLG
                     EGETSRTLSGVLYPEITGGRLSLTAIEVMANEGRAWPLIDGLGVILGMYVIEKITHTH
                     TELFNDGAARKIEFSMSLKRVDDSVAAIYGDLKTQADNLLSSAGNMLGGLTG"
     gene            complement(3937..6378)
                     /locus_tag="ON345_002494"
     CDS             complement(3937..6378)
                     /locus_tag="ON345_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3072543.1"
                     /translation="MSNNVRLEVLLNAVDKASRPLKAIQTASKSLSGDIRNSQKTLRD
                     LNAQASRVDGFRKVSAQLAVTGQSLTKAKQEAAALAVQFKNTQAPTVAQARALEAARK
                     SAADLQVKYNGLRQSVQRQRSELAQAGINTRTLSADERRLKSSISETTAQLNRQRDAL
                     ARVGQQQARLSAVKERYASGQQLAAGARNAGMVGVGVATAGLYGASRFIAPGIGFDKQ
                     MSGTQAILGLDKNDDQLAAIRKQARDIGATTAFTPGDVARTQTTLARSGYDAGAVLAA
                     TGSTVNLSLAADVDIAEAADIITNMQSAFNLSTTEIERVADVMTKGFTSSNTGLVELG
                     EAMKYVAPIAEAAGASIEDTTAMLGILADNGIKGSMAGTGASAMFNRLQAPMGKAVDA
                     IKELGVKTRDSKGNMLPVEKILKDIHTSFAKNKLGTAEQGEYLKVIFGEEAMKGAIKL
                     VAAAGDGSLASKREQIAGSKGTTERIAKIQTDNLDGDLKNLQSAWEDLQIEVFEKEDS
                     ALRRLTVSATDLLGKVAAWAKANPELTQTLFNVVTGALALVGVLGGIGLIAWPVIAGI
                     NGIIAAAGLLSVVFSTAGTAIVAAVGAISLPVLAVAGAVVAGVLLIRKYWEPLGAFFS
                     GVVEGLKAAFAPVAEMFAPLSPIFDAISEKLRMVWQWFKDLLAPVKATQETLDRCKNV
                     GVAFGQALASALTAPLDIFNSLSGKVGWLLEKLGVIKKESSDLDQTAARADGATQNGS
                     YIPATASHAGYQAYQPVTAPAGRSYIDQSKSEYNINLGGGVAPGGSLDRQLRDAVEKL
                     DREKRARQRSSMMHD"
     gene            complement(6368..6490)
                     /locus_tag="ON345_002495"
     CDS             complement(6368..6490)
                     /locus_tag="ON345_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR3072544.1"
                     /translation="MADIAVIFHWPPSELYSLSVTELITWREKALQRSGKYHEQ"
     gene            complement(6523..6858)
                     /locus_tag="ON345_002496"
     CDS             complement(6523..6858)
                     /locus_tag="ON345_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017372771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR3072545.1"
                     /translation="MANPKNKKPEFTDTAGNEIETENPNEVILDTPLMRGEQKIERVT
                     VSKPNAGTLRGVSLAALAQSDVDALIKVLPRMTYPALTEHEVARLDASDLLQFAGKVV
                     GFLSNASAR"
     gene            complement(6922..7440)
                     /locus_tag="ON345_002497"
     CDS             complement(6922..7440)
                     /locus_tag="ON345_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR3072546.1"
                     /translation="MALPRKLKYLNMFNDGLSYMGVVESVTLPKLTRKLEKYRGGGMP
                     GAVSIDLGLDDDALSLEWTLGGLPDIELWAQYAAPGADSVPLRFTGSYQRDDTGAISA
                     VEVVMRGRHKEYDGGENKQGESGSTKMSTECAYYKLTIDGKEVIEVDVINMVLVVDGV
                     DRLAEHRKAIGL"
     gene            complement(7453..8646)
                     /locus_tag="ON345_002498"
     CDS             complement(7453..8646)
                     /locus_tag="ON345_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCR3072547.1"
                     /translation="MSDYHHGVEVIEINDGTRTISTVSTAIIGMVCTASDADEKTFPL
                     NEPVLITSVQSAIAKAGKLGTLSASLQAIADQCKPVIVVVRVAEGTEDPDDAEAALKQ
                     TISNIIGTTDENGKYTGLKALLTAKTVTGVKPRILGVPGMDSQEVATALASTCQSLRA
                     FGYISAWGCKTISEAINYRDNFGQRELMVIHPDFLAWDTTANETTTAWATARALGLRA
                     RIDQETGWHKTLSNVGVNGVTGVSASVSWDLQDKATDANLLNEAGVTTLIRNDGFKFW
                     GNRTCSDDPLFLFESYTRTAQVLADTMAEAHAWAMDKPITATLIRDVVEGINAKFREL
                     KTNGYIVDGKCWYDPESNDVATLKAGKLYIDYDYTPVPPLENLTLRQRITDTYLANLS
                     ESVNS"
     gene            8869..9450
                     /locus_tag="ON345_002499"
     CDS             8869..9450
                     /locus_tag="ON345_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HCR3072548.1"
                     /translation="MLKYLLAMNFILFGDTHELFAYWKHEVIRRDKLSLHRLLREKKH
                     FGRNYLFWWRLANQMYIRGSKLEKKAAKQLNAHLITKFGCEISLGAQIGKGLTIPHHH
                     GIVIHHAVSIGENFVIRQNTTIGQKDGESKSLILHIGDNVDIGANTCIIGLGHNIGDN
                     VKIGAMSLVNSDIPDDCTFITAKTSRLITHNGL"
     gene            complement(9498..10073)
                     /locus_tag="ON345_002500"
     CDS             complement(9498..10073)
                     /locus_tag="ON345_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR3072549.1"
                     /translation="MTFEMTAQNRTITIYNYRADTLEFIGEGDALIPPYTGLPANCTN
                     KPAPETQPGFIAIFDDKNEQWKTIEDHRGETVFNTQTGEPIHISEPGSYPEGVTVLAP
                     DNTWQKWNGEVWVDDVLAKKNALIEDAILQKAALLKQATEEISTLQDAVDLSIASDDE
                     AERLQYKKFYRVMLSRVNTSDAPDIVWPEID"
     gene            complement(10073..11788)
                     /locus_tag="ON345_002501"
     CDS             complement(10073..11788)
                     /locus_tag="ON345_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3072550.1"
                     /translation="MTVKYKTVITKAGAAKLAAATLPNGKKVNFTAMAVGDGGGKLPE
                     PDAGQTKLVNEVWRHALNKISQDKKNKNYVVAELVIPPEEGGFWMRETGLYDDTGTLI
                     AVGNMAESYKPELAEGSGRAQTVRMVIMVSDIESVELAIDTTLVMATQDYVDDKLAEH
                     EQSRRHPDASLKEKGFVQLSSATDSESETLAATPKAVKAAYDLAKAKYTAQDATTTQK
                     GVVQLSSATESDSETLAATPKAVKAANDNANRRVPSTRKVNGKALSGDIELHSSDIFS
                     VATPIGNAANLNDYTTAGLYYQPANVQAQTGANYPEAVAGSLEVYKHAGITQIYRVYN
                     SSRSYVRSCYSNTWSLWAMQYDSANAPAETFPTGAPIAWPSDSIPTGYAIMQGQTFEK
                     TAYPNLALAYPAGIIPDMRGWTIKGKPATGRAVLSQEEDGIKSHTHSASASNTDLGTK
                     TTSTFDYGTKTTSTFDYGTKTTSNTGAHTHSIPTSDGANQGGKIPLTSGRLATQYSSA
                     GTGSAGAHAHTVGIGAHNHTVGIGSHTHTVAIGAHGHTITVNAAGNTENTVKNIAYNY
                     IVRLA"
     gene            complement(11800..12330)
                     /locus_tag="ON345_002502"
     CDS             complement(11800..12330)
                     /locus_tag="ON345_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCR3072551.1"
                     /translation="MSKARLLPTGSSPLEVAAAQACADIENTPIPLRRLWSPDDCPVN
                     LLPWLAWAFSVDRWDSGWPEETKRDVIRNAWFIHAHKGTIGAVRRVVEPLGYLINVSE
                     WWETSDPPGTFRLDIGVLETGITEEMYYEMERLIADAKPASRQLIGLNIIQDVPGSLY
                     VGALTYDGDIITIYPG"
     gene            complement(12323..13231)
                     /locus_tag="ON345_002503"
     CDS             complement(12323..13231)
                     /locus_tag="ON345_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HCR3072552.1"
                     /translation="MAIVDLSQLAAPDVVEELDYETILDERKATLVSLYPEAQQEAIA
                     RSLTFESEPLVKLLQENAYREVIWRQRVNEAARAVMLAYANGADLDQLGANSSVERLV
                     ITPADETTLPPTPAVLESDTDYRLRIQQAPEGLSVAGSTGAYQFHGRSADGRVADISV
                     ISPEPACVTVSVLSRESNGAASDELLAVVRAALNDEDVRPVADRVTVQSAGIVDYTIE
                     AALYLYPGPESEPVLDAAKEKLQAYITAQHRLGRDIRKSAIYAALHVEGVQRVELAAP
                     VADIVLDATQASYCTAYSVTVGGNDE"
     gene            complement(13237..13587)
                     /locus_tag="ON345_002504"
     CDS             complement(13237..13587)
                     /locus_tag="ON345_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCR3072553.1"
                     /translation="MTVRYLGMNSQTGLSISEAEHIRQSVRDILVTPIGSRVMRREYG
                     SLLSVLIDLPQNPALRLQIMTVCYSAIQKWEPRISLTSITFERAENDGALYVDITGTR
                     ETSGQPFSLTIPLS"
     gene            complement(13584..14225)
                     /locus_tag="ON345_002505"
     CDS             complement(13584..14225)
                     /locus_tag="ON345_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR3072554.1"
                     /translation="MNNLNSLQEIARAIRNLIRTGIVTDVDPVEGLCRVQTGGMKTTW
                     LNWLTCRAGRSRVWWAPSVGEQVLLLAIGGELDTAFVLPGIFSDDNPAPSASPDAFHI
                     SFPDGAVIEYEPESGALTVSGIKSADVTASDSITATVPVVMVKAETRITLDTPEVVCT
                     NKLITGSLEVQKGGKMTGDIEHTGGKFTSNGVQVDDHDHGGVKGGDEWTKGVK"
BASE COUNT         3123 a         3964 c         3706 g         3536 t
ORIGIN      
        1 cagattaaat taatcttgac atcatagctt tcaagacccg tcattttttc ccgtgcggta
       61 aactgaatac tggtaacttc tttcccggtc tttttcttaa gttcaataat tttttttgtt
      121 atatattcag aaatatctgc ttctgctttt gtttttaagt tttcaatatt catcatttcc
      181 tctttcagtc tgttatgact ttccagttac acagtaagtc gattatatgg tgcaaacgtg
      241 taaaagataa gatgaaacat cgcaataatc aacatacgat agtctaaatt ttacacaaac
      301 agacaaagag aattttcctg aattatcaat acaatagcat caaatcaact caagagcctt
      361 attgctgctt ccagaatttc ttctgaagta acatgtcgat ccgcggctac ataaatgact
      421 ttatgatctc cggtcagaga tggaaaccct gcggccatta cagtaaggtg tgttttttcg
      481 ccatttggat attcacgcat gatggtgtta actccagtca tcgctggcac taccactgct
      541 ggttcagagt taaaaaaaac tatgattttt ttcatgatgt taccgtagta tgtgagtatc
      601 catcgaatag ataccaagca aaaaagctcc cgaaggagct ttttaaaacg cttcaacata
      661 atgaaataaa aggatttatt tcaggtcacg tccacatgtt gaccacatcg ttacactaag
      721 ccccgtttgc ggggcttttt acaaattatc gttgatgtac aaatcacatg tggcaagcta
      781 tgagatgctt tcgaccgtcg gctaaaagcg ataagcaaac ctttatcttc ctggaacaaa
      841 acggacggcg tcgattaagt gattacccca gtaaagcagg atacgcggtt tcgctgctca
      901 ataaatcctt tcaccgatgg tgcactataa aaagtctgga ctttaaataa agcttttagt
      961 ctcgccacac caacaatatc cattcccata tcatagtaaa gtattagaaa taacttgttg
     1021 gaaaactatt ctattccggt ttgaatgact taaacttgac gaagattgag cataagtttg
     1081 ttgggagaat agtaccacca tcaaatccac cgttattttc cgctagccca gcacttccaa
     1141 atgagagcaa aattgcttta tcgaactcgc tacctggatg attatcgtta ttgcagtgac
     1201 taaaaacttc caatttctca atatcaaatg caggaatacg ttcaagtgca accatcaaac
     1261 ggcgaagtgt aataaaatcg ataaaatcac aagcgaatgc cttgaggaac gaagctgcaa
     1321 tttccggttt attctcgctt tccaacctat cgatgatttc aataaggcgc tgacctgctt
     1381 tgcccgcaaa attatcgtca ccgttcaatt tttcagtcat attgattcgc tgggcatcgc
     1441 ttagatcaga aaaattaaac aaaaaacgaa ttagtttttc cgtgaaaaga taatctcgga
     1501 cactacctat agcacctgaa actgcaaata tagtcttaat tacgggtatc tcttttagca
     1561 gaccactatc cattaaagag tctacagcaa tttcgccata gtccttacct aaatcggata
     1621 attgatcagc atttaaagcg tccactaatg cggagccacg cttggtgtct ttttccataa
     1681 atcctcattt atatatcatt gtgaaatatc aggtattgat tgtgaaaaac ccgcaaggga
     1741 aattatgtga ctctttcgta gtatcgttcg atcaagagag tggcgactaa gttaagtgaa
     1801 tagcttaatc ataatgcgct caataatgta atgtttcaaa tattttgcaa tataattatg
     1861 ttctgcaaga acaggtttga agaaaaaaat cttcagaatc atctgcccag acagcatgat
     1921 ttgccactgt tacgtaatta ataccacagt tctgtaatat actaaaagcc ccaaaaaaac
     1981 ggggccatgc ttggttacat ccagtgtatc tgttgctgcc ctgacgttgt cgggtgcggc
     2041 ggggctggta ctacttcacc cggagacaca ataaagcggt cgaccgtttc agtggtcaca
     2101 aatgtcgcac tgcagttgat gttagtgcac tgatggtaac gctctttcgt cgtgtctgaa
     2161 aaatagcggc ttgtgcgagc gtgtgcggca aaatggcatt tagggcagtg aaacatggtg
     2221 agcacctctt attaatttcc gatgcgctaa ttttaaccat taaaacctta tataacaaac
     2281 acttaaatca caactcttga tttaactctt cgctttcgta ctccacatcc gaaaccttaa
     2341 cctcaagctc taagcccgtc gtgtagccgt ttccgttgag gttatgcacc acccgactga
     2401 ttagccatgc ctgctcgtct ataacgcgct taaagccttt caccgcaacc ggcgtttcag
     2461 gaaataaatc cgcgcgcccg gtggcaagcg tgatagaaaa ttccgccacg ccgcgctgta
     2521 ttttatccca cttcgcctga gccgctcgca tggcctgcgc ctttgtggcg tagatggtcg
     2581 tcagctctaa cacattgtca gactcaccgg ccatatactc gccctcgcgc gcctcctgct
     2641 ctttcttcgt tttggccttg gccgtggttt tggcggcttt cgggtgctgc agcgcgcgca
     2701 ggtgctgctc tttgggttta cgtttcagct tcaccttttg tttttgcggc ttcgggtctt
     2761 tggtgtgcag ccattttgcc gtaacgccgg tgtacgcctc gcggtcggcg atagcgaacg
     2821 agtgccgatc gccgtcgccg cgttcaaccg tcatttgcgg gatgggtttg ccactggccg
     2881 tgacgccgct cccggctttc aggaaaagca gcttaccggc ttttatcgag accgttgcgc
     2941 cgttccgctc cgcaagccgg gatatgaata ccgcgtccga ttcctgcgac tggtcggcgt
     3001 gcggcaccgg gatcgctttc agggtgtcgg caacgctggc cgtcaatttg ttgcgctgag
     3061 caatggcctc aacaacctgc ccgagcgtgg tgtcatgcca tgactgctcc cggcgtgagt
     3121 tgagcgagcc gcgaaaatcc gcgctgcgcc cccggatggt cagcgtatca ggtgcgcccc
     3181 ggtgctcaat ttcatccacg gtgaaactcc ccttattcag cagcgccgag ccctcccagc
     3241 cgagccagag cgtcagggaa gcaccgcgcg gcggcagctc gaccagcccg tcggaatcgt
     3301 cgagctcaat atcgagctgg tcggcctcga atccgcgatt gtccgtcatg gtcaggccga
     3361 tgagcctgtc gctaaaattc tgcgtgatgt cgttgccgtc gagcgtgagc ataaacgccg
     3421 gggcaatacg cgccccggcc tcaatcttaa tccccgatat catcccgtca gccctcccag
     3481 catgttaccg gcagacgaca gcagattgtc cgcctgcgtt ttcaggtcgc catagatggc
     3541 cgcgacggaa tcatccacgc gcttcagcga catactgaat tcaatttttc tggccgcacc
     3601 gtcgttaaat agctcggtgt gggtgtgggt gattttctcg atgacgtaca tcccgaggat
     3661 cacaccgagc ccgtcaatca gcggccatgc gcgcccctcg tttgccatca cctcgatggc
     3721 tgtcagtgac aggcgaccgc cggtgatttc ggggtaaagc acaccggaca acgtgcgcga
     3781 ggtctccccc tccccgagaa actggtaagc cggtgtttta ccgatgcggt catttgacgc
     3841 ccaccggtag tccttcgaat actgcattga ctgatacggc agcgtgcgcc gctcaaagac
     3901 aaataacccc agtaccatca gcatgtttcc ccctccctaa tcgtgcatca tgcttgagcg
     3961 ctgacgggcg cgcttctcgc ggtcgagttt ctcgacggcg tcgcggagct ggcggtcaag
     4021 gctcccgccc ggtgccacgc cacccccgag gttaatgttg tactcgcttt tgctctggtc
     4081 gatgtaagac cggccagcag gtgctgtgac cggttgatac gcctgatatc cggcatgact
     4141 tgcggtcgcc gggatgtaag acccgttttg cgtagcgccg tcggctcggg cggcggtctg
     4201 gtcaaggtcg ctggattcct ttttgataac gccgagcttt tccagcagcc agccgacctt
     4261 gccgctcagg ctgttgaaga tgtcgagcgg tgccgtcagc gccgaggcca gcgcctgacc
     4321 aaatgccacg ccgacattct tacagcggtc gagcgtttcc tgcgtggctt ttaccggcgc
     4381 aagcaggtct ttaaaccact gccagaccat gcgcagtttc tccgaaatgg cgtcaaatat
     4441 cggcgagagc ggcgcgaaca tttccgccac cggcgcaaag gcggctttca gcccctccac
     4501 cacgcccgag aagaatgccc cgagcggctc ccagtatttg cggataagca gcacgccagc
     4561 gacaaccgcc ccggccacgg ccagcaccgg cagactgatt gcacccacgg cggcgacaat
     4621 cgcggtgcct gcagtgctga aaaccacgct cagcagaccg gcggcggcaa taatcccgtt
     4681 aatcccggcg atgaccggcc atgcaattag cccgatgcct cccagcacgc cgaccagcgc
     4741 cagtgcgccg gtgacgacgt taaacagggt ttgcgtcagt tcagggttag ctttcgccca
     4801 ggcggcaacc ttgccgagca aatcggtcgc ggaaaccgtc aggcggcgca gtgcagaatc
     4861 ttctttctcg aagacctcaa tctgcaaatc ttcccatgct gactgcaggt ttttcagatc
     4921 gccgtcgagg ttgtccgtct ggatttttgc gattcgctcc gtggtgcctt tcgacccggc
     4981 aatctgctcg cgcttgctgg cgagagaccc gtcaccggca gcggcaacga gtttaatcgc
     5041 gcccttcatg gcttcttcgc cgaaaatgac cttcagatat tcgccctgct cagcggtgcc
     5101 gagcttgttt ttcgcaaagg acgtatgaat atctttcagg attttctcga ccgggagcat
     5161 gttccctttt gagtcgcggg ttttgacgcc gagctcttta atcgcatcga ctgctttccc
     5221 catcggagcc tgcaggcggt taaacatggc gctcgcgccc gtaccggcca ttgagccctt
     5281 gataccgtta tccgccagaa tcccgagcat ggccgtcgta tcttcgatac tggcaccagc
     5341 cgcctctgcg atcggcgcga cgtatttcat cgcctcgccc agctcaacca gtccggtatt
     5401 ggaggacgtg aaacctttgg tcatgacatc cgccacgcgt tcaatttcgg tcgtggacag
     5461 gttaaaggct gactgcatat tggtgatgat gtcggcggct tcagcgatat ccacgtcagc
     5521 cgcaaggctc aggtttaccg tcgacccggt cgccgccagc acagctccgg cgtcatagcc
     5581 tgaacgggcg agcgtcgtct gcgttcgcgc cacgtcaccc ggtgtaaagg cggttgtcgc
     5641 cccgatgtca cgcgcctgtt tgcggatggc cgcgagctgg tcgtcgttct tatcgagacc
     5701 gaggatcgcc tgcgtgcctg acatctgctt atcaaagccg atacccggcg cgataaagcg
     5761 tgacgcgcca tacagcccgg cggttgccac accgacaccc accatcccgg cattacgcgc
     5821 accggcggcg agttgttgcc cggatgcgta ccgttctttc acggcgctca gtctggcctg
     5881 ttgctgaccg acgcgcgcca gtgcatcacg ctgacggtta agctgtgcgg tcgtctcgct
     5941 gatactggat ttcaggcggc gctcatccgc cgacagcgtg cgggtattaa tcccggcctg
     6001 agcgagctcg ctgcgctggc gctgcacaga ctgacgcagc ccgttatatt tgacctgcag
     6061 gtcggcagcg gatttcctgg ccgcttcaag tgcgcgcgcc tgcgccaccg tcggtgcctg
     6121 cgtgttttta aactggacgg ccagcgcggc ggcttcctgt ttcgcttttg tgagcgactg
     6181 cccggtcact gcaagctgtg cgcttacctt cctgaagccg tcaacgcggg aagcctgcgc
     6241 gttgaggtcg cgcagggttt tctgtgagtt gcggatatcg ccggacaggg atttactggc
     6301 ggtctggatt gccttgagcg gtcggcttgc cttgtcgacc gcgttcagca atacctcaag
     6361 cctgacgtta ttgctcatgg tattttccgc ttcgttgcag cgccttttcg cgccatgtga
     6421 tgagctcggt cacgctcagg gaatagagct ctgatggcgg ccagtgaaaa atcaccgcga
     6481 tatccgccat caggtcatca accgataact ggtcggggaa cgttaacgcg ccgaagcatt
     6541 cgacaaaaaa cccaccacct ttccggcaaa ctgcagcagg tcggacgcat ccagacgggc
     6601 gacttcatgc tcggtcagtg ccggatacgt catacgcggc agcaccttaa tcagcgcatc
     6661 aacgtcagac tgcgccagcg ccgccagcga cacgccgcgc agggttcccg cattgggctt
     6721 agacacggtg acgcgctcga ttttttgttc accgcgcatc agcggggtat cgaggatcac
     6781 ctcgtttggg ttttcggttt cgatttcgtt tccagccgta tcggtgaatt caggtttctt
     6841 gttttttggg tttgccatga tgtttgtctc tgctctgaaa gggagtgatg accggccaga
     6901 cgcgctgacc ggctcagggt gttacaggcc gatagccttg cggtgctctg ccagccggtc
     6961 gacaccgtcg acaaccagca ccatgttgat gacgtcaacc tcgataactt ccttgccgtc
     7021 aatggtgagc ttgtagtaag cgcactcggt cgacattttg gtcgagccgc tttcgccctg
     7081 cttattctcg ccgccgtcgt actctttgtg acggccacgc atgaccacct caacggcaga
     7141 aatcgcgccg gtgtcatcgc gctgataaga gccggtgaag cgtaatggca cgctgtcagc
     7201 acccggcgcg gcatactgcg cccacagctc aatgtcaggc agaccgccga gcgtccactc
     7261 aagcgacagc gcgtcgtcat cgagaccgag gtcaatcgac accgcgcccg gcatcccgcc
     7321 gccgcgatat ttttcaagct tgcgggtcag cttcggcagg gtgacggatt caacgacacc
     7381 catatagctg aggccatcgt tgaacatgtt cagatattta agtttgcggg gtaacgccat
     7441 gtttaaggct ccttagctgt tgaccgattc cgacaggttc gccagatagg tatcagtgat
     7501 gcgctggcgc agggtcagat tttccagcgg gggaacgggg gtgtagtcgt aatcgatata
     7561 cagcttgccc gctttcaggg tggcgacgtc gttcgattcc gggtcgtacc agcatttccc
     7621 gtcaacgata tagccgttgg ttttcagctc gcggaatttg gcgttaatgc cctccacaac
     7681 gtcacgaatc agcgtcgcgg taatgggctt atccatcgcc cacgcgtgcg cctctgccat
     7741 agtgtcggcc agtacctgcg cggtgcgggt gtagctttca aacaggaata acggatcgtc
     7801 agagcacgtg cggttgcccc agaacttaaa gccgtcgtta cgaatcaggg ttgtgacacc
     7861 cgcctcgtta agcaggttgg cgtcggtggc tttgtcctgc aaatcccacg acaccgaggc
     7921 gctgacgccg gtcacgccgt taacgcccac gtttgacagc gttttatgcc agccggtctc
     7981 ctggtcgatt ctggcacgca gacccagcgc gcgcgccgtc gcccatgcgg tcgtggtttc
     8041 gttcgccgtg gtatcccacg ccagaaaatc agggtgaatg accatcagct cgcgctgacc
     8101 gaaattgtcg cggtaattga tggcctcgga aatggtcttg cagccccacg cgctgatata
     8161 gccaaacgcg cgcagactct ggcaggtgga ggcaagcgcg gtcgccacct cctgagaatc
     8221 catccccggc acaccgagaa tgcgcggctt cacgccggtg acggtcttcg ccgttaacag
     8281 cgccttgagg ccggtgtatt tgccgttttc gtcggtcgtg ccgatgatgt tggaaatggt
     8341 ttgtttcagc gccgcttctg cgtcgtccgg atcttcagta ccttcagcaa cgcgaacgac
     8401 cacgataacc ggcttgcact ggtcggcgat ggcctgcagt gaggccgaca gggtgccgag
     8461 cttacccgct ttcgcgatag cactctgcac gctggtaatc agtaccggct cattgagggg
     8521 aaacgttttt tcgtcagcat cgctggccgt gcagaccatg ccgatgattg ccgtcgagac
     8581 ggtggaaatg gtgcgcgtgc catcgttaat ctcgatgacc tcaacgccat gatgatagtc
     8641 gctcatccgt ttaactccgt ggttatgggg tgagcgtatt gtctggcgag tacctggaag
     8701 gggctactta tcggagttgg ctggggaatg acacaacgct gattattaat caacctatca
     8761 aaatttgaat caataaattg cgaagaattt atgcaatcat atgccgggta aacgtagtac
     8821 gcggatacat aatcaaaatt tatacaaaaa tagctatcag ggttggtcat gcttaagtac
     8881 ttattggcaa tgaatttcat tctttttgga gacacacacg aactttttgc ttactggaaa
     8941 cacgaagtta tcaggaggga taaactctca ttacataggc ttttacgtga gaaaaaacac
     9001 tttggccgta actacctctt ctggtggcga ctggcgaatc aaatgtacat tcgtggtagt
     9061 aagttagaaa agaaagcagc taaacagctc aatgcccatt tgataactaa atttgggtgt
     9121 gagatttcac tgggggcaca gatagggaaa gggcttacaa tcccgcatca tcatggaatt
     9181 gtaattcatc atgctgtaag catcggtgag aattttgtca ttcgccagaa tacaaccatt
     9241 ggtcaaaaag acggcgaaag caaatcgcta atattacata ttggcgataa tgtggatatc
     9301 ggagcaaata cgtgcattat cggcctgggt cataatatcg gtgataatgt aaaaattggc
     9361 gcaatgtctc ttgtgaatag tgacattcct gacgactgca cattcattac ggcgaaaaca
     9421 agtcgactta tcacgcataa cggcttatga aaaattataa gttagcctga taatctcagg
     9481 ctaactcttg aagatagtta gtcgatttca ggccagacaa tgtctggtgc atcactcgta
     9541 tttacgcgac tcaacatgac tcgataaaat ttcttatact gcaatcgctc cgcttcatca
     9601 tctgatgcta tgctcaaatc cacagcgtcc tgcaaggtag atatctcctc tgttgcctgt
     9661 tttagcaatg cggccttttg caaaattgca tcctcaatga gtgcattctt cttagccaga
     9721 acatcatcaa cccagacctc tccattccat ttttgccatg tattatctgg cgctaataca
     9781 gtaacacctt caggatagct tcccggctct gatatgtgta ttggttcacc tgtttgagta
     9841 ttaaaaacgg tttcccctct gtgatcttcg attgttttcc actgctcatt tttatcatca
     9901 aaaatcgcga tgaaccctgg ttgagtttcc ggggctggtt tatttgtgca gttggcaggc
     9961 agcccggtat aaggtggaat aagagcatcc ccttcaccaa tgaactccag cgtatctgca
    10021 cgatagttat aaattgttat agttcggttt tgagccgtca tttcaaaagt catcaggcaa
    10081 gcctcacgat gtagttatat gcgatgttct ttacggtatt ttcagtattc ccggcagcgt
    10141 ttactgtgat ggtgtgaccg tgcgcgccaa ttgcaaccgt atgggtatga gacccaattc
    10201 ccacagtgtg gttatgagct ccaatgccaa ccgtgtgcgc gtgtgctccg gctgagccag
    10261 tacctgcaga tgaatactgt gtagccaggc gtccagatgt gagcggaatt ttcccaccct
    10321 gattagcgcc atctgaggta ggtatcgagt gtgtatgcgc acctgtatta ctggtagttt
    10381 tcgtaccgta atcgaatgtg ctagttgttt tggttccgta gtcaaaagtg ctggttgttt
    10441 tcgtgcctaa atcagtattt gatgcgctgg cgctatgcgt atgcgattta atgccatctt
    10501 cttcctgcga caatactgct cgcccagttg ctggcttacc ttttatcgtc cagccacgca
    10561 tatctggaat aatgcctgca gggtaagcaa gggcgagatt agggtaagcg gttttctcaa
    10621 atgtctgccc ctgcataatg gcgtatccag tcgggataga gtctgacggc cacgctattg
    10681 gtgctcccgt ggggaaagtt tcagcaggtg cgttagctga gtcatactgc attgcccaca
    10741 atgaccaggt attggagtaa cacgacctaa catagcttct tgaactgtta tacacgcgat
    10801 aaatctgcgt gatcccggca tgtttatata cttccagcga accggccacc gcctccggat
    10861 aatttgcccc ggtctgtgcc tgtacgttcg ctggctggta atagagacca gccgtggtgt
    10921 aatcattcag atttgctgca ttaccaatag gcgttgctac tgagaaaata tcagaagaat
    10981 gcagttcaat atcgcctgat agcgcctttc cattaacttt ccttgttgaa ggtacgcgcc
    11041 tattggcgtt gtcgtttgcc gccttaaccg ctttcggcgt cgctgcgagc gtctcagaat
    11101 cactttcggt ggcactcgat agctggacta cccccttttg cgttgtggtg gcgtcctgag
    11161 ccgtgtattt cgctttcgca aggtcatacg ccgccttaac cgcttttggc gttgctgcga
    11221 gcgtttcaga ctcactgtcg gtggcgctgc tgagctgcac gaaacctttt tctttcagcg
    11281 aggcgtcagg gtgtcggcgt gactgctcat gctcggcgag cttatcatcg acataatcct
    11341 gcgtggccat caccagcgtg gtatcgatgg ccagctcgac cgactcgatg tcgctcacca
    11401 tgataaccat gcgcaccgtc tgcgcgcggc ctgagccctc ggccagctcc ggcttataac
    11461 tttcggccat attgccgacc gcaatcagcg tgccggtgtc gtcatagagc ccggtttcgc
    11521 gcatccagaa tccgccctct tccggcggga tgaccagctc ggccacgaca taatttttat
    11581 tctttttgtc ctggctgatt ttattcaggg catgacgcca gacctcatta accagctttg
    11641 tttgcccggc gtcaggctcg ggtagtttcc cgccaccgtc gccgacggcc atcgccgtaa
    11701 agttcacttt cttcccgttg gggagggtcg ccgccgcaag tttcgccgca ccggctttgg
    11761 tgattaccgt tttatatttc actgtcatgt tgctctcact tagcccggat agatggtgat
    11821 aatgtcgccg tcataggtca gggctccgac gtacagggag cccggcacat cctgaataat
    11881 gttcaggccg atgagttggc ggcttgccgg tttggcatca gcaataagcc gctccatttc
    11941 gtaatacatt tcctcagtga tacccgtttc cagcacgcca atatccagcc ggaaggtgcc
    12001 gggcgggtcg ctggtctccc accactcgga cacgttaatc aggtagccga gcggctcaac
    12061 aacgcggcgc accgcgccaa tcgttccctt gtgggcatgg ataaaccacg cattgcggat
    12121 cacatcgcgt ttcgtttcct ccggccagcc actatcccag cggtcaaccg aaaacgccca
    12181 ggcgagccac ggcagcaggt tgaccgggca gtcgtcagga ctccacaacc ggcgcagggg
    12241 gatcggagtg ttttcgatgt cggcgcaggc ctgcgccgcc gccacctcaa gcggcgacga
    12301 gcccgtcggc aataaccggg ctttactcat cattaccccc gaccgttacg ctgtacgccg
    12361 tgcagtacga ggcctgagtc gcatcgagca cgatatcggc caccggcgcg gccagctcga
    12421 cacgctgcac accctcgacg tgcagcgcgg catagattgc agacttacgt atatcccgcc
    12481 cgagccggtg ctgcgcggtg atataggcct gcagtttctc ttttgccgca tcaagcaccg
    12541 gctcactttc cgggccagga tagagataga gcgccgcctc gatggtgtaa tcgacaattc
    12601 cggctgactg cacggtcaca cggtcagcca ccgggcgaac gtcctcatcg ttcaacgctg
    12661 cacgcaccac ggcgagcaat tcgtcagacg ccgcgccgtt gctctcccgc gatagcaccg
    12721 acacggtgac gcaggcgggt tccgggctga taactgaaat atctgcgacc cgcccgtcgg
    12781 cgctgcggcc atgaaactga taagcaccgg ttgacccggc cacgctcagc ccttccgggg
    12841 cttgctggat acgcagacga taatcggtgt ccgattccag cacggcaggt gtcggcggca
    12901 gcgtggtctc atctgcaggg gtaatgacga ggcgctcgac gctggaattt gcccctaact
    12961 ggtcgaggtc tgcgccgttg gcataggcca gcatgaccgc acgtgcagcc tcgttaacac
    13021 gctgacgcca gataacctcc cggtaggcgt tctcctgcag cagcttcaca agcggttcag
    13081 actcaaacgt caggctgcgc gcgatcgcct cctgttgcgc ttcgggatag agagagacga
    13141 gcgttgcctt gcgctcatca aggatggttt cataatccag ttcttccacg acatcaggcg
    13201 cggcgagctg gctcaaatca acaattgcca tagcgtttaa ctcagtggaa tggtgaggga
    13261 aaagggctgg ccggacgtct cacgcgtgcc ggtaatatcg acatagagcg cgccgtcatt
    13321 ctctgcgcgc tcaaaggtga tggatgtcag gctgatacgt ggctcccact tctggatcgc
    13381 ggaatagcac acggtcataa tctgcagtcg cagcgcaggg ttttgcggca ggtcaatcag
    13441 cacggaaagc agcgagccgt attcacgacg catcacccgc gagccaatgg gcgtgacgag
    13501 aatgtcgcgc acgctctgcc tgatgtgctc agcctctgaa atactgaggc cggtctggct
    13561 attcatgcca agataacgca ccgtcattta acccccttag tccattcatc gccgcccttc
    13621 acaccgccgt ggtcgtggtc atccacctgc acgccgttgg aggtaaattt cccgccggtg
    13681 tgctcaatgt cgccagtcat cttcccgccc ttctgcactt cgagtgagcc ggttatcagc
    13741 ttgttggtgc agacaacctc cggcgtatcg agcgtgatgc gggtctcggc cttgaccatt
    13801 accaccggca cggtcgctgt tatggagtca gacgcggtaa cgtcagcgga tttgataccg
    13861 gacacggtga gcgccccgct ttcgggctcg tactcgatga ccgcaccgtc agggaatgag
    13921 atatgaaagg catcgggtga ggctgacggc gcggggttgt catccgagaa aatgccgggc
    13981 agcacaaacg cggtgtcgag ttcgccgccg atagccagca aaagcacctg ctcgcccacc
    14041 gagggagccc accacacccg cgaacgaccg gcacgacagg tgagccagtt cagccaggtt
    14101 gttttcatgc ccccggtctg aacacgacac agcccctcga cggggtcgac gtcggtcaca
    14161 atgccggtgc gaatgaggtt gcggatcgcg cgtgcgattt cctgcagaga atttaaatta
    14221 ttcatggggg aaaggatgcc gccgggtagg gccagcggca atcgaggcag gttttgtcag
    14281 atatggcaca acaccccaat aattttaaag attgtatctc accaccatc
//
LOCUS       DAJSKJ010000098        14282 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000098 DAJSKJ010000000
VERSION     DAJSKJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.098"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(106..774)
                     /gene="artM"
                     /locus_tag="ON345_002506"
     CDS             complement(106..774)
                     /gene="artM"
                     /locus_tag="ON345_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR3072555.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPVLWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(774..1490)
                     /gene="artQ"
                     /locus_tag="ON345_002507"
     CDS             complement(774..1490)
                     /gene="artQ"
                     /locus_tag="ON345_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR3072556.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1497..2228)
                     /gene="artJ"
                     /locus_tag="ON345_002508"
     CDS             complement(1497..2228)
                     /gene="artJ"
                     /locus_tag="ON345_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3072557.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2246..2974)
                     /gene="artP"
                     /locus_tag="ON345_002509"
     CDS             complement(2246..2974)
                     /gene="artP"
                     /locus_tag="ON345_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR3072558.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3192..3707)
                     /locus_tag="ON345_002510"
     CDS             complement(3192..3707)
                     /locus_tag="ON345_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3072559.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3833..4156
                     /locus_tag="ON345_002511"
     CDS             3833..4156
                     /locus_tag="ON345_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR3072560.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4153..4983
                     /gene="amiD"
                     /locus_tag="ON345_002512"
     CDS             4153..4983
                     /gene="amiD"
                     /locus_tag="ON345_002512"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR3072561.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4980..5993)
                     /locus_tag="ON345_002513"
     CDS             complement(4980..5993)
                     /locus_tag="ON345_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3072562.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6092..7522)
                     /locus_tag="ON345_002514"
     CDS             complement(6092..7522)
                     /locus_tag="ON345_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR3072563.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASERRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7533..8534)
                     /gene="ltaE"
                     /locus_tag="ON345_002515"
     CDS             complement(7533..8534)
                     /gene="ltaE"
                     /locus_tag="ON345_002515"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR3072564.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8571..10289)
                     /gene="poxB"
                     /locus_tag="ON345_002516"
     CDS             complement(8571..10289)
                     /gene="poxB"
                     /locus_tag="ON345_002516"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR3072565.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10422..11390)
                     /gene="hcr"
                     /locus_tag="ON345_002517"
     CDS             complement(10422..11390)
                     /gene="hcr"
                     /locus_tag="ON345_002517"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR3072566.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11402..13054)
                     /gene="hcp"
                     /locus_tag="ON345_002518"
                     /gene_synonym="priS"
     CDS             complement(11402..13054)
                     /gene="hcp"
                     /locus_tag="ON345_002518"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR3072567.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIDLREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13198..14097)
                     /gene="lysO"
                     /locus_tag="ON345_002519"
     CDS             complement(13198..14097)
                     /gene="lysO"
                     /locus_tag="ON345_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR3072568.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3440 a         3824 c         3774 g         3244 t
ORIGIN      
        1 aggcgtccac gccgcatccg gcacaggcac cgtgctgatg tctgatgcga cgctggcgcg
       61 tcttatcaga cctacaaacc cccccggcga atgtacgcag ctacattaat tccgccgttc
      121 gaatgccagt gctttgcgct cgatcagacg catcatcagc gtcagcaggc cgttgacgac
      181 caggtaaata atccctgccg caccgaacac cattacatcg taggtgcgtc cgtacaacaa
      241 ctggctgtat cccatcactt ccatcagcgt aatggtgtaa gccagagagg tacttttgaa
      301 caccagcacc acttcgttgg aataagaaga gagcgagcgt ttaaaggcat acggcagcag
      361 gatcgccagc gtatcttttt tgctcattcc cagggcgcta caggactgcc actgaccttc
      421 tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta tacgccgcac tgttcagcga
      481 taacgcaatc agcgcacata accacggttc tgacaacaaa tgccacagta ccggatactc
      541 ctgcaaagtc ggaaactggc ccggcccgta ataaatcagg aagatctgca ccagcagcgg
      601 cgtaccggta aacagcgtga tataaccccg caccagccac accagcaccg gcgttttcag
      661 cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc acaatcagcg aggcaacggt
      721 tagcgtcagg ctggtgtgta gccctttcat cagttcgggt aaatactcaa acattagctg
      781 ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt tgagaatgta ctgactgagc
      841 agggtgatca ccaggtaaat cgccgccgcc acaatgtacc aggtaaatgg ttcctgggta
      901 cgagtagcga tgctttttgt ttgcagcatt aaatcattca cactaatcaa actgaccagc
      961 gcggtatctt tcagcagcac cagccactgg ttaccgaggc caggcagcgc atgacgccac
     1021 atctgcggca tcaccagacg gaaaaagata gccgatttcg acagccccag cgcctgacca
     1081 gactcccact gacccaccgg caccgctttc aacgcgcccc gaagcgtttg cgaggcatag
     1141 gcggcataca gcagtgacag agcgatgaca ccacaaagga acgggctaac gtcgaagttc
     1201 tcaatgtcca tctgcactgg gatctgcacg aacccaagat tgatagtgaa gccatccgaa
     1261 agcgtcagca gcagctgcga ggagccaaaa tagataaaca gcaccaccag aatttctggc
     1321 aggccacgca gaatggttac cagcgctgaa cctgcccacg cgacaggacg ccattttgcc
     1381 gactcccata ccgcaaagaa catcgccagc gccagcccga caatcaatgc acaaacggca
     1441 aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa attcattcat caggaattac
     1501 ttctggaacc atttgttgta gatggtttcg taagtgccat ctttcttcac tttttccagc
     1561 gcagtgttga gtttctgctg cagctcagtg ttgccctgac gtaccgcgat gccgaggcca
     1621 gtgccgaagt aatctttatc ggtcactttg tcgcccaccg ccgccagttt cgggttatct
     1681 ttcagccact cagtgaccac tgcggtgtca ccgaagacgc cgtcgatacg cccgttttgc
     1741 agatccagtt ttgcgttctg gtagctgtca tacggaacgg tagtgatttc cgggtgctta
     1801 tccataatga atttctggtg tgtcgtcccg ttctgtacgc cgactttttt gcctttcagc
     1861 tgatcaacac tggtgtattt gccttgctga cccacaaaca gggcagagtt gtcatagtac
     1921 ggggtggtaa acagcacctg cttttcacgc tccggagtga tatccatgcc cgccatcacg
     1981 gcttctacgc gacggaattt caggcttggg atcaggctgt caaacgcctg gttagagaaa
     2041 gtgcaggttg catcaatctc tttacacagc gcttgtgcca ggtcgacgtc aaaaccaacg
     2101 atctggttgt ttgcatcaat cgattcaaac ggaggatagg aggcttcggt agcaaaacga
     2161 atggtttcgg cagctgtggc ggaaagacta aaacctgcaa ttaacgcggc aatcagaact
     2221 tttttcattg ttgttatccc gaatcttagt gagagagata gtttttaaat gcttcggttt
     2281 gcggctcggt aaagcagctc gcgtcgcctt gttctacgat atgaccattt tccatataca
     2341 ccactcggct ggcggtttta cgcgccactt caacttcgtg ggtgacgatc acctgggtaa
     2401 tattcgtttc tgccagctca cgaatgatgc tgacgatttg tgccgtaatt tccgggtcca
     2461 gtgcggcggt cggttcatcg aacagcagta cctgcggttc catcatcaac gcacgggcaa
     2521 tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg gtaacgatcg ctataaggtt
     2581 tgagacgcag acgttccagc agtttttctg cacgggccag cgcctgatct ttactcaacc
     2641 ccagtacacg gcagggcgct tcaatcaggt tttgctgcac ggtcagatgc ggccacaggt
     2701 tgtattgctg aaacaccatg ccaacgttac gacgcaaatc gcgaatcgct ttgtcagagg
     2761 gtgttttggt gaaatcgaaa tggttgcctg caatgttgag cgtaccggag cgcggcatct
     2821 caagcagatt gagtacacgc agcagcgagc ttttacccgc gccgctgggg ccaagtaaca
     2881 ccagcgtttc gccctgtggg caatccagcg tgatatcgaa cagcgcctga tgcgcgccgt
     2941 agaagcaatt aatgccgttt aattgaatac tcattgacac tcgtatactg gcagtctgat
     3001 agctattgag gtcgaagata gtacctttga cagaataatt atgcaatatt tatgctttaa
     3061 aagttaaaag caaagcgcat tattcaataa acatagcaca aaataacggg ggcggtggtc
     3121 ggtgagcata aatgtcggca ttcctcacga aatgccggac aatttacggg gtttattggt
     3181 tgatcaaggc gttagcgatt ctcgatggac tgacggagcg tacccgccgt ggcatgaacg
     3241 ctgccgccta aatagcgtac atcgtcgatc acccaacact ggccttcctg aatcattaac
     3301 acttcatcct gccagctctg atcaccctgt tttagatcca cacgcagagg aatatttcgc
     3361 gcatcacgat tcgggattgt tgaggcactg gcaacacggg cgctctctgg caaggttgtg
     3421 cgactggaga atggatcgtt ggtcagtagt tcccgatggt tattatcccg ggaggcatcg
     3481 ctaagcagtg tcgccagttt gtcgctcaga taagggcgca aggcggtgat gtcgttgctg
     3541 cggtgcaaaa tgcggtagtc ataaaattgc tgggccacgt tatccgggcc tccttcaacg
     3601 caaggaccac tgcgtgtgcc gttatcttta taagctggag tgactgtggt gcaggcactg
     3661 aggagcagtg cgcaggggat aagcattgtc aatttgctgt agcgcataat gatttcctta
     3721 taagcgatcg ctctgaaagc gttctacgat aataatgata tcctttcaat aatagcgtat
     3781 cagtctgata atgcttttga gatcgaaggc ttagcaaaca aggagatcga tcatgcaatt
     3841 ttctacaacc ccaactctgg aaggccagac catcgttgaa tattgcggtg tggtgaccgg
     3901 cgaagcgatt ttaggtgcca atattttccg tgatttcttt gccggtatcc gcgatatcgt
     3961 tggcggacgt tccggtgcgt atgaaaaaga actgcgtaaa gcacgggaga tcgcctttga
     4021 ggaattaggc tcccaggcgc gggcgctggg ggccgatgcc gtcgtcggta ttgatatcga
     4081 ctacgaaacg gtcgggcaaa acggcagtat gctgatggtt agcgtcagcg gtacggcggt
     4141 gaaaacgcgt cgatgagaag agtcttctgg ctggtcgctg ccgctctgtt attggcaggg
     4201 tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatatc agcttgatac ccgacgccag
     4261 gcgcaggcgg cgtatccgcg cattaaagtg ctggtgatcc actacaccgc agatgatttt
     4321 gatagctcgc tggcgacact gaccgataag caggtcagct cgcattatct ggtccctgcg
     4381 gtaccaccgc gatacaacgg taaaccgcgc atctggcaac tggtgccgga acaagaactg
     4441 gcctggcatg cggggattag cgcctggcgc ggggcaacgc gccttaacga cacctctatt
     4501 ggcattgagc tggaaaaccg tggctggcaa aaatcggccg gagtgaaata ttttgccccg
     4561 tttgaaccgg cacagattca ggcgcttatt ccactggcga aagatattat tgcccgttat
     4621 cacatcaagc cggaaaacgt agtggcacat gcggatatcg caccgcagcg caaagacgat
     4681 ccggggccat tatttccctg gcagcaactg gcgcagcagg ggattggtgc ctggccggat
     4741 gcgcagcggg ttaactttta ccttgccggg cgcgcgccgc acacaccggt agataccgcg
     4801 tcattgctgg agcttttggc gcgctacggt tatgacgtta aacctgatat gacaccgcgc
     4861 gagcagcggc gcgtgattat ggcattccag atgcatttcc gcccgacgtt atataacggc
     4921 gaagcggatg cagaaactca ggcgattgcc gaagcattgc tggagaaata cgggcaggat
     4981 tagcgcggca gttttccgtg gtcgcgtagc caggcggcag ttttctcgat accttcatcc
     5041 agggtgatga ccggctgata acctaactct tcctgcgcac gcgtaatatc cagcgtaaag
     5101 tcaaaattca acttggagac gccgtagtgg gtcagcggcg gctcttttgc tgacttgcgg
     5161 cctaaacgct ccatgctgcg ggcgatcata tccagcatcg ggtaggggac ggaacgaata
     5221 cgacagtcaa tattcaactc gtcgatcagc ttctgcacga tgctgcgcag tgtgcgatgc
     5281 tcgccgttgg tgatgttgta cacacgcccg gaaggtagct tatcgcaggc ttcctggctt
     5341 gccagccaca ttgcgtgcac ggcattttca tagtaggtca tatcgaccag cgcactgccg
     5401 ccatgcggta acagaatact gccgtagtgg tgcatcatat gcgccagacg gggaataaag
     5461 actttatcgt gcggtccgaa cagactttgt gggcgcagaa tagtaaagcg cgtttgtgga
     5521 ttcgcctgcg aaagcatatt gatcacttct tcgctggctg ctttgctgcg ggcaaactcg
     5581 ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc gatggtggtg ataatcgaag
     5641 tacagggagg gggaagagat atgaataaag ttacgcacac cccaggcgac agcccattca
     5701 cccaggcggc gagtggcgcg aacgttagcc agatcgaaag cctgttgtgt cccccagggt
     5761 gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg cgagcatcac tttagcttgt
     5821 gatgaaacca gctcggtcag atccgccgga acaaactctg cgcccatttt ttccagcaat
     5881 ttgcccattg cctcgttgcg accggtcgct cgcacgctga tgcctttctg gcataaaaac
     5941 tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa ccagtacctt catatcaatc
     6001 cactgttgtt gagaaaataa cgtgcgcatt cttccgttat ttcccccatg atgcaatggg
     6061 aaacatgaaa gaataacgca ggttttgtcg attaatctgt gctttgttct gccagtctgg
     6121 cgatttgttt tgccattccg cgaaaaataa acagatgcgc ggggatcatc aataaccagt
     6181 aaaacagccc cggcataccg tgcggatgcc agaaagcgcg gacatcgata gtacgatagt
     6241 cgcctttatc ttccaggcta aaacacagtc gtcccagccc cggcgctttc atgccaaata
     6301 acaacgtaag ttgtttttcc ggttcaacga caatcacttt ccagctatct accgcatcgc
     6361 cagtctgtaa atattcgcgc tccgggcggc ctttcgccag cttatgaccg atcgcgcggt
     6421 ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa ataacgctct ttaccgccga
     6481 tttggttcac tacctgccat aaagcagcaa ggctggcgga cgttttaacg gtaaaccccg
     6541 cctgtttggc aaaataaccg tactccggtc gccagcgggc aaaggcctga gcgtcgtagc
     6601 cccagtcgct ggagttgacc agtttttctt cctctttcaa cgtgctacgt accgcgtcat
     6661 cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc ggtatcatcc gccagcagat
     6721 cgtgtttcag cccctgaatc aacgccctgg cggtggtggg cggtacggaa gtaatcacat
     6781 tgagaaacca caccgaaatc cagcgggtgg ggagggggat ggggatcaac cagcggcgct
     6841 taccgctcac cgccataaaa tgttcaaact gttgctgata actgagcacc tctggtccgg
     6901 cggcttcgaa gatgcggcgt tcgctggccg gatgatctaa caacgccacc agatagtgca
     6961 gcaagttttc cagcgcgatg ggcgtggtgc gtgaacgtac ccagcgtggc ggcgttaaca
     7021 ctggcaggtt gtagaccata tcgcgcatga cttcgaacgc cgctgaacct gcgccaacga
     7081 taattccggc ccgaagttcg gtcacaggta cattcgcttc acgaagaatg tccgccgtag
     7141 cctgacgagc acgcagatga tccgactgct catgtggcgg ggcctgcaac gaactgagaa
     7201 agattaattg cttaactggt acttcacgta gcgcatcgcg gacgttgaga gccacctggc
     7261 gctcctgagc gataaaatcg ccgccttcgc ccatgctgtg caccagaaaa tagacggtat
     7321 cgatatcctg caacagggcc ggaaggttat ccggccagct gagatcgact ttatggcaac
     7381 tgacgtttgc cagttgcagc tttgcaagcc tgtcgacatg acgtgccgcc gccaggatct
     7441 gatgcccttg ctggctgagt gtgcgcacca gatgctgacc aatgtagcca ctggcaccga
     7501 gaactaaaat gcgttgcggc acgtctctct ccttaacgcg ccaggaatgc gcgccagtgg
     7561 gcggcgactt ccgccagttg ttcgcgcgag acgtcaagat gcgtcaccag gcggacaatc
     7621 ggcgaggcgt taatcagcac gtttctcgct ttcatgtatt cgcctaacgc ggcagcattt
     7681 tcttccccga cgcgaacaaa cagcatatta gtgtcctgac gcatcacatc cgcgcctgct
     7741 tcacgcagtt gctccgccat ccaggcggcg ttgtcgtgat cttcctgcaa ccgtgcgacg
     7801 ttatttttca gggcatacat cccggcggca gccagaatcc cggactgacg catcccgcca
     7861 ccggccattt tccgccagcg gatggcacgt ttaatgtaat cacgattacc gacgagtaat
     7921 gaaccgactg gcgtcccaag acctttcgac aggcaaatgg tgaacgaatc acaatattgc
     7981 gtgatctctt tcagttcgca gccgtaagcc accacggcat taaagatgcg cgcaccgtca
     8041 acatgcagcg ccagattgcg ctcgcgggta aattcccatg cttctttcag gtattcacgc
     8101 ggcagcactt tgccgttgtg ggtgttttcc agactgagta atttggtgcg ggcgaaatgg
     8161 atatcgtcgg gtttgatttt catcgccact ttatccagcg gtagcgtgcc gtcggcagcc
     8221 gcgtcgatgg gttgcggctg aatactgccc agcaccgctg cgccaccggc ttcaaacaga
     8281 tagttatgcg cggcctgacc gacaatatac tcttcgccac gttcgcagtg actgagcaga
     8341 gcgaccaggt tggcctgagt gccggtaggc agaaaaatgg cggcttcttt accggaaagc
     8401 tctgctgcat agtcctgcag agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca
     8461 accggggcgg ccatcatcgc ttcgagcatg gcgcggctcg gtcgggtaac ggtatcactg
     8521 cgtaaatcaa tcatggcatg tccttattat gacgggaaat gccacccttt ttaccttagc
     8581 cagttcgttt tcgccagttc gatcacttca tcgccgcgtc cgctgataat tgcgcgcaac
     8641 atatacaggc taaaaccttt ggcctgttcg agtttgatct gcggtggaat ggctaattct
     8701 tctttggcga ccaccacatc caccaacacc ggaccgtcga tagagaaggc gcgttgcagg
     8761 gcttcatcaa cttcagaggc tttttctaca cggatacccg taatgccgca cgcttcggca
     8821 atgcgggcaa agttggtgtc gtgcagctca gtaccgtctg tcaggtaacc tccggccttc
     8881 atctccatcg ccacaaagcc cagcacgctg ttattaaaga cgataatttt caccggcagt
     8941 ttcatctgca ctactgagag gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac
     9001 atggcgacca cctgacgttc cggctcggtt gcctgcgcac ccaacgcctg cggcatggcg
     9061 ttagccatcg aaccgtggtt aaacgaacct aacaggcgac gcttgccgtt cattttcaga
     9121 taacgtgccg cccataccgt tggcgtgccg acgtcacagg taaagatggc gtcatcggcg
     9181 gcaaaatgac taatttgctg cgccagatat tgcgggtgaa tggctttctc gctcggttta
     9241 gctaaatcgt ccagcccttt gcgggcatcg cggtaatctt ccagcgcttt atccagaaac
     9301 ttgcgatcgg ctttttcttc caccaatgga agcaatgcac gcagagtcga cttgatatcg
     9361 ccgaccagtg ccatatccac cttgctgtga gcgccgatgc tggctgggtt gatatcaatc
     9421 tgaatgattt tggcatcggt cgggtagaag gcgcggtagg gaaattgcgt gccgagtagc
     9481 actaacgtgt cggcgttcat catggtatgg aaacctgacg agaagccgat taacccggtc
     9541 attccaacat catacggatt atcgtattcg acatgttctt taccgcgcag ggcatgaaca
     9601 ataggcgctt taattttccc ggcaaactca actaactctt tatgcgcccc cgcgcagccg
     9661 ctgccacaca tcagggcgat attgctggaa taacgcagca gttgcgccag tttgcgtaac
     9721 tcttcttctt ccggcgtcac gactggttgt ggcgcatgat accagtgcgt ggttgcccct
     9781 tctggcgcag gttttaacgc cacgtcgcct ggtaacacga caaccgaaac gccacggtta
     9841 agcaccgctt tgcgcatggc aatcgccagt acttgtggga tctgctccgg gctggaaacc
     9901 agctcgcaat agtgactaca ttcgcggaat agctcttgtg ggtgggtttc ctggaaatag
     9961 ccgctgccaa tttcgctgga gggaatatga gcggcaatcg ccagtaccgg aacgtgattg
    10021 cggtggcaat cgaacaggcc gttgattaag tgcaggttgc cggggccgca cgatccggcg
    10081 cagaccgcca gttctccgct aagttgtgct tcagcgccag cggcaaaggc cgccacttct
    10141 tcgtggcggg tggacatcca ctcgatggtg cccatgcgat taagactgtc actaagaccg
    10201 ttcagagagt cgcctgtgac tccccagatg cgtttcaccc ctgccgattc gagtgttttg
    10261 gcgatataag ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt gataacggta
    10321 acaagtttag ttcatctgac ggagggggaa gggatgggag agaaaggagg cactaacggt
    10381 taaatagccc gatgaaagga atatcatcgg gcataaggcg attatgcgag aaccaaatcc
    10441 ccctgcggat ggcaggagca ggccagtacg taaccttcag cgatttcggc gtcggtcagc
    10501 gtcattgtac tgctcaccgt atattcaccg gaaaccactt ttgtcttaca gcagccgcaa
    10561 acacccgcac ggcaggcagc gacaaccgga acgttattgc tttccagcgc ctccagtagc
    10621 gtggtgccaa ccggggcgta aaattctcgt gccggttgca gtttggtgaa tttcagaccg
    10681 ctggtcgccg cttctgctac tggggtgaag aatttctctt taaagaaacg cgtcacgccg
    10741 agcgctttca cttcctgctc tacccaatcc atatacggag ccgggccgca ggtcatcacg
    10801 gtacgtgaag ctaagtcagg tacacctgcc agcagttcgc gagtgagacg accagcgata
    10861 aagccttcgg taacgttatt ttctgccacc agcgttaccg gatagttacg ccactcatcg
    10921 gcgaaaataa catcctgcgg cgtacgcacg ttgtagatca cccgcacatc ggcctgtgga
    10981 cggttcttcg caagccagcg acgcatcgac ataatcggcg tgacgccgca gcctgccgcc
    11041 agcaacagga atttatcttc tgctttatcg tcgcaggtaa attcccccat cgcgtccgaa
    11101 agccagagat aatcaccgcg ttttacatcg cgcgtcagcc actgggagcc gacaccgtca
    11161 tcaatccgcc gcacggtcag ggtgatatat tcactcacgc ctggcgtgga ggaaatggtg
    11221 taagcacgca gcgtttccgc tgagtttcgc acgctgacca gtgcatattg cccggcgcga
    11281 tatgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt ttcttgcgta
    11341 atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat cgacaaactc
    11401 cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa cgcaggccga
    11461 atttctcgtt cagcaccgca agcaggtctg gtgtcaggaa accaggtgca gtcgggccgg
    11521 tgacgatatt tttcacgccc agagaaagca gcgtcagcag aatgacgatc gctttctgtt
    11581 caaaccagga gagcaccagc gacagcggca gatcgttgac accgcagccc agtttctctg
    11641 ccagagtgac agccagaata atcgctgagt aagcatcgtt acattgacct gcatctacca
    11701 gacgcggcag accttcgata tcgccaaact caagtttgtt aaagcgatat ttaccacagg
    11761 cgagggtcag gatcaggcag tcatccggca cgctggtggc gaaatcggtg aagtagtggc
    11821 gctcgccgcg tgcgccgtca cagccaccaa gcaggaagat atgacgcagt ttttcacggc
    11881 tcaccagatc aatcagcgta tccgctgcgc caagcagcgt ctggcggcca aagcccacgg
    11941 tgatcaggtg cggaatttcg ctgtacggga agcccgccat ttgttgcgcc tgggcgatga
    12001 ccgcagaaaa atcttcgcca tccagatgac gcacacccgg ccagccaacg atgctgcggg
    12061 tccagatacg atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc gaggtcatca
    12121 cgatggggcc agggaaacga gcgaactcca cttgctgatt ctgccagccg ctgccgtagt
    12181 taccgaccag atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc agcatttcgc
    12241 cgtgggtgta aacattaacg cccgtgcctt cggtctgttc cagcaggttg tagagatctt
    12301 tgagatcgtg accggaaatc aggatgcatt tacccgccgt cgctttgacg ttgacctggg
    12361 ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcatc cagaatgctc atcactttga
    12421 agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg gcaggccagg
    12481 tccccagcca cgccatgatt ttatgatact gggcataaat atcgttgtcg tattgaccga
    12541 gaacatgcgc gtgttccata taggccgccg cacctttcag gccatacagg cacagcagac
    12601 gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta aattctgctg
    12661 cctgacgttg cagctcgccg agatcgtcgc tcaccagttg caggtcagcc atcgggttat
    12721 cgacgcgcgc gttggcatct acagccaggc attgtgcttt cagcgcctcg cgcaggtcaa
    12781 tcgcttcacg agcgtagccg actatacgcg gagaatcgaa gttaacgttg gtcagggttg
    12841 agaaaaatgc gcgtggcgca aagctgtcga catcgtggtt gatgatgccg tattcacgcg
    12901 cttttaccgc ccaggccgaa agcccttgca gcgccgcaat gagtaaatcc tgaaggtcag
    12961 aggtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt cctgccggag
    13021 tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa gttatattta
    13081 atatacatgt ttaaggttaa gacgcttaac gcggggataa aagggatttt taatgcaact
    13141 ttaagggaga ttgatttagc gcaattttgg cggcagagat ctaccgccag agaggtatta
    13201 cgcggagaaa aaggcgatga ggatcggcac taacaggcta agaataaaac cgtgaacaat
    13261 tgccgccggg accatatcca ggccgccagt acgttgaaga acgggcaggg tgaaatccat
    13321 cgacgtcgca ccgcataagc ccagtgcggt agagcggctg cggcgaatca gcccagggat
    13381 caacataata gcaatcagtt cacgggccag atcattaaaa aacgccgcgc tcccgattac
    13441 cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga aaccggaggc
    13501 cattgccagc gcggtattga tgggtagatc aagaataaag gcgttaatta atccaccaat
    13561 taatgagctg gcaaccacca ccacggcgac aatcattccc cggcgattaa ggacaatctg
    13621 ctttaaggtc atgccattat tgcgcaactg aataccaacg aggaaaagta gtaaaattaa
    13681 cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca gaccaatggc
    13741 aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg caatacgcga
    13801 cgggagtttt tcttgctgat ggtggttgcg ccacggcagg cctcgctcca gccacatcag
    13861 ggcggcaata ttacacagta aaataacggt aatactgacg gcagaataat gcagaatcgc
    13921 caacaggtta ctggcgaggt tatcgagaaa cgccagactg atacccataa aaaagagaat
    13981 aaggtaaacc atccagctta atagctgatt aataactttt aacgcagctt gttggcgaag
    14041 cggaatgagg taacccacaa tcaggggaac cagaatgatt aacagcccag aaaacatgaa
    14101 aacccagtcc ttgcaaagat gaagtcgaaa tgcgcgatga cacactactg aaagcggaag
    14161 gacgagtaaa gttgcaatta aaaggaaatg ttatgcataa ggagcagtag agtattcgtt
    14221 ttcatttaaa gatattcttg cgctttaatt acaaactgca ccgatgttgg tggcgtcaaa
    14281 at
//
LOCUS       DAJSKJ010000099        14218 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000099 DAJSKJ010000000
VERSION     DAJSKJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.099"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..843)
                     /locus_tag="ON345_002520"
     CDS             complement(<1..843)
                     /locus_tag="ON345_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3072569.1"
                     /translation="MTSDKTLKQAISSITIWRKGEQRAPHKPLLLLHVLSHYRQGHDR
                     LFNYGSEIYEPLLDLLERYGPQRRDQRPDMPFWRLKGDGFWELQNAELCSTNGSRQPP
                     RRELIEYNVAGGFDADSFGLVAKNHRLIDTLAKQILEAHFPASIQEEIADEMGFDIRT
                     SLRQRDPKFRQMVLRAYNYQCAICGFNMRHDNAPIALEAAHIRWKQHHGPCEVPNGLA
                     LCAIHHKAFDRFSIGLDEDMRVVVSGAVNGGGVVQRLFWDFAGKAIALPPVKDNYPGE
                     RFVEW"
     gene            complement(1060..2325)
                     /locus_tag="ON345_002521"
     CDS             complement(1060..2325)
                     /locus_tag="ON345_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR3072570.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGSGMHLLVHPNGSKYWRLQYRF
                     DGKQKMLALGVYPEITLADARARRDEARKLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNQKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASGNRQHRPALALKRIPELLQKIDG
                     YTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPDVKHSHRGS
                     KMHTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPRKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEFIDERKLML
                     QWWADFLDANRDKGITPFDYAKINQGHGE"
     gene            complement(2512..2596)
                     /locus_tag="ON345_002522"
     tRNA            complement(2512..2596)
                     /locus_tag="ON345_002522"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2560..2562),aa:Leu,seq:caa)
     gene            2792..3811
                     /gene="ahr"
                     /locus_tag="ON345_002523"
     CDS             2792..3811
                     /gene="ahr"
                     /locus_tag="ON345_002523"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR3072571.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            3941..5443
                     /locus_tag="ON345_002524"
     CDS             3941..5443
                     /locus_tag="ON345_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR3072572.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQIMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5562..6644)
                     /gene="lptG"
                     /locus_tag="ON345_002525"
     CDS             complement(5562..6644)
                     /gene="lptG"
                     /locus_tag="ON345_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR3072573.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDSDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6644..7744)
                     /gene="lptF"
                     /locus_tag="ON345_002526"
     CDS             complement(6644..7744)
                     /gene="lptF"
                     /locus_tag="ON345_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR3072574.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDDSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            8011..9522
                     /gene="pepA"
                     /locus_tag="ON345_002527"
     CDS             8011..9522
                     /gene="pepA"
                     /locus_tag="ON345_002527"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR3072575.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9656..10099
                     /gene="holC"
                     /locus_tag="ON345_002528"
     CDS             9656..10099
                     /gene="holC"
                     /locus_tag="ON345_002528"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR3072576.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10099..12954
                     /gene="valS"
                     /locus_tag="ON345_002529"
     CDS             10099..12954
                     /gene="valS"
                     /locus_tag="ON345_002529"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR3072577.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13010..14206)
                     /locus_tag="ON345_002530"
     CDS             complement(13010..14206)
                     /locus_tag="ON345_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR3072578.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
BASE COUNT         3622 a         3677 c         3668 g         3251 t
ORIGIN      
        1 ccattccaca aaccgctcac ccggataatt atctttaaca ggtggcaacg caatcgcctt
       61 tcccgcaaaa tcccagaaca gccgctgtac caccccacca ccgttaaccg caccggaaac
      121 caccacacgc atatcttcat ccaacccaat cgaaaaacga tcaaacgctt tatgatggat
      181 tgcgcacaat gccaggccat taggtacttc acaagggcca tggtgctgct tccatctgat
      241 atgggcagct tccagagcaa tcggcgcatt gtcgtggcgc atgttaaagc cgcagatagc
      301 acactgataa ttgtaagcac gtagcaccat ttggcgaaac ttcggatcgc gttgacggag
      361 agaggtacga atatcaaacc ccatctcgtc agcgatctct tcctgaatac tggccgggaa
      421 atgcgcctcc aagatctgct tcgccagcgt atcaattaac ctgtgatttt ttgccaccaa
      481 cccaaaacta tcggcatcaa atccacccgc gacattatat tcgatgagtt cacgtcgggg
      541 tggctggcgg ctaccattgg ttgagcacag ttccgcgttt tgtagttccc aaaaaccatc
      601 gcccttcaga cgccagaacg gcatgtccgg gcgctggtca cggcgctgcg gtccataacg
      661 ttccaggaga tccaataggg gctcgtagat ttcagaacca tagttaaaca ggcgatcatg
      721 accctgccga tagtgtgaga ggacgtgcag cagcaataat ggcttatgtg gcgcacgctg
      781 ttcgcctttg cgccagatag tgatacttga tatcgcttgc tttagggttt tgtcagaagt
      841 cattacattg aagaatccat gagcacatat ttgcgatgat gctcgcaact atatgtacat
      901 tcaaggcatt attgcaggca tcttttttat ctgtcagctc tgtacctacc agatggaaat
      961 ttgataataa cggtaaaaaa atacaatgac caaccagccc tcatcgggct ggttggtcat
     1021 tttaatttag acatcaaccc cgaaataacg gcactcgact cactcaccat gcccctggtt
     1081 gatcttcgca tagtcaaatg gcgtaattcc tttatcccga ttcgcatcca ggaaatcagc
     1141 ccaccattgc agcatcagtt tgcgttcgtc gataaactcc gccttatgga tatatgccgc
     1201 ccgtactgaa ttacgctcca tatggctcat ctgtcgttct accgcatcct tcgaccatag
     1261 ccctgactca atcaatgagc tacaggccat cgttcggaag ccgtgaccac aaacttcaac
     1321 cttggtgtca taccccatca cccgtagcgc actgttcacc gtgttttcac tcatcggctt
     1381 acgcggatcg tgatcgccaa taaaaatcag ctcatgctcg ccacagaatt gttttatctg
     1441 cttcaggatc gccagtgcct gcttagaaag cggcaccaaa tgcggtgtat gcatctttga
     1501 tccccggtga gaatgcttaa cgtcagggat aggctctctc tctggtggga tcgtccacat
     1561 cgacgtttca aaatcaatct ccgaccaacg agcaaaacgt agttcactgg atcgaataaa
     1621 gataagcagg gtaagttcag ttgcccaacg ggttaatggt cttccggtat agccatctat
     1681 tttctgaagc agctctggaa tacgctttaa cgccagcgca ggccggtgct gtcggttgcc
     1741 agaagcaacc gctcctgcca tctcctgagc cggattataa tcaattaacc cgctttgcac
     1801 cgcataacgc atgatggctg tagtgcgctg ttgaagccgg gcagccacct caagacgtcc
     1861 agacatctct acggctttaa tgggcgctaa taaatcgcga gtttttaatt cagcgatatt
     1921 acgttcacca agcgctgcaa agagattatc ttccagactt tttagcactc gatgggcatg
     1981 atcttcagac cacttttgat tggtgccatg ccactcgatc gccacctctt tgaaggtccg
     2041 tgctttactc tgttcaacct tatcattttt ctttttgtct cccggatcga cgccattcgc
     2101 tagcagctta cgcgcttcgt cacgacgcgc tctggcatcc gccagtgtga tttcaggata
     2161 aaccccaagt gccagcatct tttgcttgcc gtcaaaacga tactgcaaac gccagtattt
     2221 agaaccatta ggatggacaa gaaggtgcat gccgctgcca tcggtcagct tatattgctt
     2281 atccgttggc ttggctgttc tgactttgat atctgttaac gccatgctta tcccctccct
     2341 gttggtacaa gcattatcga accggaaata ccatcatctg taccaacatg tgttggtaga
     2401 tgtacgttga tgtcggttga ccttgagaga gttaatatag cgggaaaagt gggtaaatac
     2461 tggattttag acataaaaaa agacctcagt tgaggtccat ttacatgctt ttggtgccga
     2521 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     2581 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     2641 cgccaccatg caagcgccag cttgctcgca cctcgctaag tgctgaaatt ttcagcattg
     2701 cataccgcga tgtgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     2761 ttcccagaca acaccagaga aggaccaaaa aatgtcgatg ataaaaagct atgccgcaaa
     2821 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     2881 tgttgaagta caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgacaa
     2941 tgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtggt
     3001 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     3061 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     3121 cgagcaaggt gcggtgccga cgattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     3181 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     3241 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     3301 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     3361 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     3421 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag cactggcggg
     3481 gcagtttgat ctcattatca acaccgtcaa cgtcagcctc gactggcagc cttattttga
     3541 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     3601 tccggccttt acgttaattg cgggcgatcg cagcgtctct ggttctgcta ccggcacgcc
     3661 ttatgagctg cgtaagctga tgcgttttgc cgcccgcagc aaggttgcgc cgaccaccga
     3721 actgttcccg atgtcgaaaa ttaacgacgc catccagcat gtacgcgatg gtaaagcgcg
     3781 ctaccgcgtg gtcctgaaag ccgacttctg atccctttgc cgggtgcctg tcgcccggca
     3841 cttaaatctc tttggaaggc gtctcgcaat ctgctgaatt tagcggtgaa gagtgcacag
     3901 ccgttgccta tactccaggc aggaaactgg aggatatctc atgagcgaac ccctgttaat
     3961 tgcccgcacg ccggacacag aactgttttt actgccggga atggctaacc gtcacgggct
     4021 gattactggc gcaacgggga cgggtaaaac tgtcacgctg caaaaactgg cggagtctct
     4081 atcggaaatc ggtgttccgg tgtttatggc cgatgtgaaa ggcgatctga ccggcatcgc
     4141 gcaggcagga acggcatcgg aaaaactact cgcaaggctt aaaaatatcg gcgtcaatga
     4201 ctggcaaccg catgctaatc cggtggtggt gtgggatatc tttggcgaga aaggccatcc
     4261 ggtgcgggcg acggtttcag acctggggcc gctgttgctg gcgcggctgt tgaatctcaa
     4321 cgatgtgcaa tctggcgtgc tgaatatcat cttccgtatt gctgacgatc agggattgtt
     4381 gctgctcgac tttaaagatc tgcgggcgat tacccagtac atcggcgata acgccaaatc
     4441 tttccagaat cagtacggta atatcagtag cgcatcggtt ggtgccatcc agcgcggact
     4501 gttgtcgctg gaacagcaag gcgcagcaca cttctttggc gagccgatgc tggatatcaa
     4561 agactggatg cgcaccgata ccaacggtaa aggcgttatc aatatcctca gcgccgagaa
     4621 gctttatcag atgccgaaac tgtacgccgc cagcctgctg tggatgcttt cagagttgta
     4681 tgaacaattg ccggaagcgg gcgatctgga gaaaccaaaa ctggtgtttt tcttcgacga
     4741 ggcacatctg ctgtttaacg atgcaccgca ggtactgctg gataagattg agcaggtgat
     4801 acggcttatt cgctcaaaag gcgtaggcgt ctggttcgtt tcgcaaaacc cgtctgatat
     4861 tccggataat gtgctcgggc agctcggtaa tcgcgttcaa cacgctttgc gtgcttttac
     4921 gcccaaagat cagaaagcgg taaaagctgc ggcgcaaacc atgcgggcca atccggcatt
     4981 tgataccgaa aaggcaatcc aggaactggg gaccggcgag gcgttaatct cgtttcttga
     5041 tgtgaaagga agtccttcag tggtggaacg ggcgatggtg attgcgcctt gttcgcggat
     5101 gggaccggtg acggaagatg agcgtaatgg cttgattaat cactctccgg tgtatggcaa
     5161 gtacgaggat gaggtggacc gggaatccgc ctatgagatg ttgcaaaaag gctttcaggc
     5221 cagtaccgag cagcaaaata atccccccgc gaaagggaaa gaggtagcgg tggatgacgg
     5281 cattcttggt ggattgaagg atattttgtt tggcactacc ggaccacgcg gcggtaagaa
     5341 agatggtgtg gtgcaaataa tggcgaaaag cgccgcccgc caggtgacga atcagattgt
     5401 acgcgggatg ttggggagtt tgctgggggg gagaagaagg taagtcaggt gcggtctgtg
     5461 cccctcaccc taaccctctc cccaaagggg cgaggggacc gatcgaactc gtatttgact
     5521 ttatcaacaa tgtgaaaggg agctttcgct cccttgcttg gttacgattt tctcattaac
     5581 agccacaggc tgattaagaa gaagctggcg cttggcaaca gtgcgccgat gatcggcggg
     5641 atgccataaa ccaacgtcag cgggccgaag atctggtcca gtacgtagaa gacaaaaccg
     5701 aagctgatac cggtgaccac acgcacgccc atcggtacgc tacgcagtgg gccaaagatg
     5761 aacgacagcg ccatcagcat catcaccgcc acggatagcg gctggaagat tttgctccac
     5821 atgttgagct gataacgtcc agcgtcctga ccgctcgact tcagatactt cacgtagttg
     5881 tgcaaaccgc tgattgagag tgcatccgag tccagcgcca ccacgcccag tttgtctggc
     5941 gtgaggttgg ttttccaggt gccgctcacc gtctgcgaac cggtaatctg tttcggattg
     6001 gtcagatcag attcatcaac ctgcgacaga cgccagactt tatgttccgg gtcaaacttc
     6061 gcagtagcgg catagcgtac ggattgcaga cgacgatttt cgttaaaggc ataaatgctg
     6121 atgccaccta actcttcgtc acctttaacc cgctcaatgt agacgaagtt gttgccatct
     6181 ttcgcccata agccttgctg ggtagagagc aacgagccgc cgtacatcgc ctgcgcacgg
     6241 tagttacgcg ccatctgctc gccctgcggc gcgacccatt caccaatcgc catcgtcagc
     6301 aagactagcg gaatggcggt tttcatcacc gacagcgcaa cctgcatacg ggtgaaacca
     6361 gaagcctgca tcaccaccag ttcgctgcgc tgcgccagca tcccaagacc aagcaacgcc
     6421 ccaagcagag ctgccatcgg gaagaagatt tgcacatctt tcggcacgct cagcaaggta
     6481 tacattcctg cgcctaacgc gtcgtaactc ccctgcccgg cttttttcag ctgatcgaca
     6541 aatttgataa tgcccgacag cgacaccagc atgaacagcg tcatcatgat ggtggtgaaa
     6601 atagttttac cgatatagcg gtcaagtacg ccaaaaggtt gcatcacacc gctcctttac
     6661 gcgaaaaact ggcgcgcagg cggcggaccg gcacggtgtc ccaaaggttg agaacaatcg
     6721 ccaaagccag ataaatcagg ttaacggtcc acatccacag cgtcgggtcc agcttacctt
     6781 taccgccgtt cgatttcagg gaggtctgga tcaggaagaa aagtagatac agcagcatgg
     6841 ctggcagcat cgacagtacg cgtccctgac gtgggttaac cacgctcagc ggtacgacca
     6901 taagtgccat cataaacacg gtgaatacca acgtgatacg ccagttcagt tctgcgcggg
     6961 cacgatcggt gtcagtgttc cacaatgtac gcatgtccat ctggtcggta tcgttcgggt
     7021 cgagcgccac cgcctggtga ccaatgatcg cctgataatc ctggaagtcc gtaatgcgga
     7081 aatcacgtaa cagtgcagtg ccttcgaagc gcgttccctg gttgagagtg acgacctggg
     7141 agtcgtcgcg cagctgggtt aaatgtccgg aatcggccac caccacagaa ggacgtgcat
     7201 taccttttgg tcgaatttgc gcgaggaaca catctttgaa atcgctgccg tcaacgcttt
     7261 cgatgaacag caccgaactg ccattagtcg cttgctggaa ttgcccttgc gccagcgccg
     7321 ccatgccagg gttcgctttc gcttctgcta acacttcatc ctgatgacgc gatgaccacg
     7381 gtcccgccca catcacgtta accgccgcga cgattgccgt gaataccgca aggatcattg
     7441 ccgctttcac cagaaccgct ttgctcaggc cgcaggcatg cattaccgta atttcacttt
     7501 cggtatacag tttgcccagc gtcatcagca gcccgaggaa caggcttaat ggcaggataa
     7561 gctgcgccat ttccggcaca cccaacccga gaagggagag caccagattc gccggaatat
     7621 cgccgtcaac cgctgcgccg aggatcctca ctaacttttg acagaagaag atcaaaagca
     7681 agatgaagag tatcgccagc tggcttttga gcgtctcccg caccagatat cttatgatta
     7741 tcactttaaa tacgcccgta aaaactcgtc ttttgcagga ttttagcttg tttcatggct
     7801 taaacgtcat ttattctctt gagtcgtcga aatcgtcgct aagataatta tactcaacgg
     7861 attcacctct cagattttgt tctgacgtgc caatgccgta ataacgttaa gattaacacg
     7921 aagtcatcgc aacagcggac atgagttacg aaagcttgca attctatctg tagccaccgc
     7981 cgttgtcttt aagattcagg agcgtagtgc atggagttta gtgtaaaaag cggtagcccg
     8041 gagaaacagc ggagtgcctg catcgtcgtg ggcgtcttcg aaccacgtcg cctttctccg
     8101 attgcagaac agctcgataa aatcagcgat gggtacatca gcgccctgct acgtcggggc
     8161 gaactggaag gaaaaccggg gcagacattg ttgctgcacc atgttccgaa tgtactttcc
     8221 gagcgaattc tccttattgg ttgcggcaaa gaacgtgagc tggatgagcg tcagtacaag
     8281 caggttattc agaaaaccat taatacgctg aatgatactg gctcaatgga agcggtctgc
     8341 tttctgactg aactgcacgt taaaggccgt aacaactact ggaaagtgcg tcaggctgtc
     8401 gagacagcaa aagagacgct ctacagtttc gatcagctga aaacgaacaa gagcgaaccg
     8461 cgtcgtccgc tgcgtaaaat ggtgttcaac gtgccgaccc gccgtgaact gaccagcggt
     8521 gagcgcgcga tccagcacgg tctggcgatt gccgccggga ttaaagcagc aaaagatctc
     8581 ggcaatatgc cgccgaatat ctgtaacgcc gcttatctcg cttcacaagc gcgccagctg
     8641 gctgacagct acagcaagaa tgtcatcacc cgcgttatcg gcgaacagca gatgaaagag
     8701 ctggggatgc attcctatct ggcggtcggt cagggttcgc agaacgaatc gctgatgtcg
     8761 gtgattgagt acaaaggcaa cgcgtcggaa gatgcacgcc caatcgtgct ggtgggtaaa
     8821 ggtttaacct tcgactccgg cggtatctcc atcaagcctt cagaaggcat ggatgagatg
     8881 aagtacgata tgtgcggtgc ggcagcggtt tacggcgtga tgcggatggt cgcagagcta
     8941 caactgccta ttaacgttat tggcgtgctg gcaggctgtg aaaacatgcc tggcgggcgt
     9001 gcctatcgtc cgggcgatgt gttaaccacc atgtccggtc aaaccgttga agtgctgaac
     9061 accgatgccg aaggccgcct ggtactgtgc gacgtgttaa cttacgttga acgttttgag
     9121 ccggaagcgg tgattgatgt ggcgacgctg accggtgcct gcgtgatcgc gctgggtcat
     9181 catattactg gtctgatggc gaaccataat ccgctggccc atgaactgat tgccgcgtct
     9241 gaacagtccg gtgaccgcgc atggcgctta ccgctgggtg acgagtatca ggaacaactg
     9301 gagtccaatt ttgccgatat ggcgaacatt ggcggtcgtc ctggtggggc gattaccgca
     9361 ggttgcttcc tgtcacgctt tacccgtaag tacaactggg cgcacctgga tatcgccggt
     9421 accgcctggc gttctggtaa agcgaaaggc gcaaccggtc gtccggtagc gttgctggca
     9481 cagttcctgt taaaccgcgc cgggtttaac ggcgaagagt aagtttgcgt tttaccctca
     9541 ccctaaccct ctccctaagg gagagggaac cgatcgagcg caatagaaat tcattgctca
     9601 tcttttaggg tgaggaatgc agcaatattt aaatccacca caagaagccc catttatgaa
     9661 aaacgcgacg ttctaccttc tggacaatga caccaccgtc gatggcttaa gcgccgttga
     9721 gcagctggtg tgtgaaattg ccgcagaacg ttggcgcagt ggtaagcgcg tgctcatcgc
     9781 ctgtgaagat gaaaagcagg cttaccggct ggatgaagcc ctgtgggcgc gtccggcaga
     9841 aagctttgtt ccgcataatt tagcgggaga aggaccgcgc ggcggtgcgc cggtggagat
     9901 cgcctggccg caaaaacgca gcagcagccc gcgcgatata ttgattagtc tgcgaacaag
     9961 ctttgcagat tttgccaccg ctttcacaga agtggtagac ttcgttcctt atgaagattc
    10021 tctgaaacaa ctggcgcgcg aacgctataa agcctaccgc gtggctggtt tcaacctgaa
    10081 tacggcaacc tggaaataat ggaaaagaca tataacccac aagatatcga acagccgctt
    10141 tacgagcact gggaaaagca gggctacttt aagcctaatg gcgatgaaag ccaggaaagt
    10201 ttctgcatca tgatcccgcc gccgaacgtc accggcagtt tgcatatggg tcacgccttc
    10261 cagcaaacca tcatggatac catgatccgc tatcagcgca tgcagggcaa aaacaccctg
    10321 tggcaggtcg gtactgacca cgccgggatc gctacccaga tggtcgttga gcgcaagatt
    10381 gccgcagaag aaggtaaaac ccgtcacgac tacggccgcg aagctttcat cgacaaaatc
    10441 tgggaatgga aagcggaatc cggcggcacc attacccgtc agatgcgccg tctcggcaac
    10501 tccgtggact gggagcgtga acgcttcacc atggacgaag gcctgtccaa tgcggtgaaa
    10561 gaagttttcg ttcgtctgta taaagaagac ctgatttacc gtggcaagcg cctggtaaac
    10621 tgggacccga aactgcgcac cgctatctct gacctggaag tggaaaaccg cgaatcgaaa
    10681 ggttcgatgt ggcacatccg ctatccgctg gctgacggcg cgaaaaccgc agacggtaaa
    10741 gattatctgg tggttgcgac tacccgtccg gaaaccctgc tgggcgatac tggcgtagcc
    10801 gttaacccgg aagatccgcg ttacaaagat ctgattggca aatatgtcat tctgccgctg
    10861 gttaaccgtc gtattccgat cgttggcgac gaacacgccg acatggaaaa aggcaccggc
    10921 tgcgtgaaaa tcactccggc gcacgacttt aacgactatg aagtgggtaa acgtcacgcg
    10981 ctgccgatga tcaacatcct gacctttgac ggcgatatcc gtgaaagcgc ccaggtgttc
    11041 gataccaaag gtaacgaatc tgacgtttat tccagcgaga tcccggcgga gttccagaaa
    11101 ctggagcgtt ttgctgcacg taaagcagtc gttgccgcag ttgacgcgct tggcctgctg
    11161 gaagaaatta aaccgcacga cctgaccgtt ccttacggcg accgtggcgg cgtggtcatc
    11221 gaaccaatgc tgaccgacca gtggtacgtg cgtgccgatg tactggcgaa accggcagtt
    11281 gaagcggttg agaatggcga cattcagttc gtaccgaagc agtacgaaaa catgtatttc
    11341 tcctggatgc gcgatattca ggactggtgt atctctcgtc agttgtggtg gggtcaccgt
    11401 atcccggcat ggtatgacga agcgggtaac gtttatgttg gccgcaacga agacgaagtg
    11461 cgtaaagaaa ataacctcgg tgccgacgtt gccctgcgtc aggacgaaga cgttctcgac
    11521 acctggttct cttctgcgct gtggaccttc tctaccctag gctggccgga aaacaccgac
    11581 gccctgcgtc agttccaccc aaccagcgtg atggtatccg gcttcgacat catcttcttc
    11641 tggattgccc gcatgatcat gatgaccatg cacttcatca aagatgaaaa tggcaaaccg
    11701 caggtgccgt tccacaccgt ttacatgacc ggcctgattc gtgacgacga aggccagaag
    11761 atgtccaaat ccaagggtaa cgttattgac ccactggata tggttgacgg tatttcgctg
    11821 ccagaactgc tggaaaaacg caccggcaat atgatgcagc cacagctggc ggacaaaatc
    11881 cgtaagcgca ccgagaagca gttcccgaac ggtattgaac cgcacggtac tgacgcgctg
    11941 cgcttcaccc tggcggcact ggcgtctacc ggtcgtgaca tcaactggga tatgaagcgc
    12001 ctggaaggtt accgtaactt ctgtaacaag ctgtggaacg ccagccgctt tgtgctgatg
    12061 aacacagaag gtcaggattg cggcttcaac ggcggcgaaa tgacgctgtc gctggcggac
    12121 cgctggattc tggcggagtt caaccagacc atcaaagcgt accgcgaagc gctggacagc
    12181 ttccgcttcg atatcgccgc aggcattctg tatgagttca cctggaacca gttctgtgac
    12241 tggtatctcg agctgaccaa gccggtaatg aacggtggca ccgaagcaga actgcgcggt
    12301 actcgccata cgctggtgac tgtactggaa ggtctgctgc gcctcgcgca tccgatcatt
    12361 ccgttcatca ccgaaaccat ctggcagcgt gtgaaagtac tttgcggtat cactgccgac
    12421 accatcatgc tgcagccgtt cccgcagtac gatgcatctc aggttgatga agccgcactg
    12481 gccgacaccg agtggctgaa gcaggcgatc gttgcggtac gtaacatccg tgcagaaatg
    12541 aacatcgctc cgggcaaacc gctggagctg ctactgcgtg gttgcagcgc ggatgcagaa
    12601 cgtcgcgtaa atgaaaaccg tggcttcctg caaacgctgg cgcgtctgga aagtatcacc
    12661 gtgctgcctg ccgatgacaa aggtccggtt tccgttacga agatcatcga cggtgcagag
    12721 ctgctgatcc cgatggctgg cctcatcaac aaagaagatg agctggcgcg tctggcgaaa
    12781 gaagtggcga agattgaagg tgaaatcagc cgtatcgaga acaagctggc gaatgaaggc
    12841 tttgtcgccc gcgcaccgga agcggtcatc gcgaaagagc gtgaaaagct ggaaggctat
    12901 gcagaagcga aagcgaagct gattgaacag caggctgtta tcgccgcgct gtaatcaaaa
    12961 cacagtaatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct tacagaaatg
    13021 gtaaagaagg cataattccc cgtgaaatcc tggctaagaa gcctttatct ggttgttctt
    13081 ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt tttgcctgcc
    13141 agtcaatcat ccatattttc agcagtggat aagccagacc gcccgtaatc ccggatatca
    13201 ccaccaacgc acacatgcga taaagcatac cgtggtacgt taaagttgag cgaaaacgaa
    13261 tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac accgttaaat
    13321 aactggtgct aacagcaatc ccaaaataat agattaactg cgcgattatc attttacgtt
    13381 gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcatacaca gaactatcat
    13441 acgcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa ggtaataaag
    13501 ctaaaaatgc gagaatggca tattttatac aatacgtagt atctaatttc accttgaaac
    13561 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
    13621 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
    13681 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
    13741 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc atagagcaat
    13801 taaaactaaa tcttacgccg ttgagactgg tcatcaaggc ctgataacgc agtccttttg
    13861 ctgccataaa tataagcaaa accaacagta acaaagtcaa aacacagcca acaagtggca
    13921 tatctgctga cactgtcatt aagattgcga aatagcaaaa aacaaaaaca agacaactaa
    13981 caaaaacatt cccaccagta attccataag aaaatcgttg tccgttaact tccatattag
    14041 catagagata acgcttacat ttcattaatg cccatggcaa atagatacct agcgttataa
    14101 tcgttaacaa cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa
    14161 caaacgaatg ggacggaaca tccatttcat taataacttg agccataaaa attccctg
//
LOCUS       DAJSKJ010000100        14184 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000100 DAJSKJ010000000
VERSION     DAJSKJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.100"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            12..1145
                     /gene="ompN"
                     /locus_tag="ON345_002531"
     CDS             12..1145
                     /gene="ompN"
                     /locus_tag="ON345_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR3072579.1"
                     /translation="MKSKVLAFLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSSKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1286..1720
                     /gene="uspF"
                     /locus_tag="ON345_002532"
     CDS             1286..1720
                     /gene="uspF"
                     /locus_tag="ON345_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR3072580.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1896..2831
                     /gene="ttcA"
                     /locus_tag="ON345_002533"
     CDS             1896..2831
                     /gene="ttcA"
                     /locus_tag="ON345_002533"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR3072581.1"
                     /translation="MSQNQEISKKEQYTLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2960..4333)
                     /gene="dbpA"
                     /locus_tag="ON345_002534"
     CDS             complement(2960..4333)
                     /gene="dbpA"
                     /locus_tag="ON345_002534"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR3072582.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGVDIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4811..5794)
                     /gene="zntB"
                     /locus_tag="ON345_002535"
     CDS             complement(4811..5794)
                     /gene="zntB"
                     /locus_tag="ON345_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR3072583.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6049..7281
                     /gene="dgcM"
                     /locus_tag="ON345_002536"
                     /pseudo
     CDS             6049..7281
                     /gene="dgcM"
                     /locus_tag="ON345_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(7302..7865)
                     /gene="smrA"
                     /locus_tag="ON345_002537"
     CDS             complement(7302..7865)
                     /gene="smrA"
                     /locus_tag="ON345_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR3072584.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8195..9103)
                     /locus_tag="ON345_002538"
     CDS             complement(8195..9103)
                     /locus_tag="ON345_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3072585.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9279..10589
                     /gene="abgA"
                     /locus_tag="ON345_002539"
     CDS             9279..10589
                     /gene="abgA"
                     /locus_tag="ON345_002539"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCR3072586.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10589..12034
                     /gene="abgB"
                     /locus_tag="ON345_002540"
     CDS             10589..12034
                     /gene="abgB"
                     /locus_tag="ON345_002540"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR3072587.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12071..13597
                     /gene="abgT"
                     /locus_tag="ON345_002541"
     CDS             12071..13597
                     /gene="abgT"
                     /locus_tag="ON345_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HCR3072588.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRHADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13608..14123
                     /gene="ogt"
                     /locus_tag="ON345_002542"
     CDS             13608..14123
                     /gene="ogt"
                     /locus_tag="ON345_002542"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR3072589.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT         3558 a         3572 c         3490 g         3564 t
ORIGIN      
        1 ggatttattc aatgaaaagc aaagtactgg catttttaat tcctgccctg ctcgccgcag
       61 gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat ctgtatggca
      121 aagttgatgg cctgcattat ttttctgata attcatcgaa agatggcgat cagagctatg
      181 cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca actcactggc tacggtcaat
      241 gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca tggacccgtc
      301 tggcatttgc cgggctgaaa tttgcagatt acggttcttt cgattacgga cgtaattatg
      361 gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc ggtgactctt
      421 ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact tatcgtaata
      481 ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa ggtaacaacg
      541 aaggagccag taatggtcag gaaggcacca acaacggacg tgatgttcgc catgaaaacg
      601 gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc gctggtgcgg
      661 catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg ggtggtgata
      721 aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac ctggcaacca
      781 tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgct gtggcaaaca
      841 aaacccagaa ttttgaagtc actgcacagt accagtttga ttttggtctg cgtccggcag
      901 tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cggtggtgca gacaacccgg
      961 caggtgttga tgataaagat ctggttaaat acgccgatgt tggcgcgact tactatttca
     1021 ataaaaacat gtccacctac gttgactata aaatcaacct gttggatgaa gatgacagct
     1081 tctacgctgc caatggcatc tctaccgatg atattgtcgc tttaggtctg gtttatcagt
     1141 tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc ctcctttgat
     1201 ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg ttacacttta
     1261 tgcggatact aaaacaggag gttttatgaa cagaacgatt cttgtcccta tcgatatttc
     1321 cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa agattgacga
     1381 tgcagaggtt catttcctga cggtaatacc ttcactgccc tactatgcct ctctgggttt
     1441 agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca aatcgcaact
     1501 ggaagagatc attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga
     1561 aggctcgccc aaagaccgca ttctggaatt ggcgaagaag atccccgctc atatgatcat
     1621 cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttccaacg ccgcagctgt
     1681 agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc
     1741 tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt ccccctctaa tccgtaccat
     1801 acacgccaca gtttttatat cagatttctt atgctgggcg ttccggcatg acgcatactc
     1861 ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta
     1921 agaaagaaca atacaccctg aacaaattac aaaaacgtct gcgtcgtaac gtgggcgaag
     1981 ccattgctga cttcaatatg attgaagaag gcgatcgcat catggtttgc ctctccgggg
     2041 gtaaagacag ctataccatg ctggagattc tgcgcaattt gcagcaaagc gcgccaatca
     2101 atttttcgct ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg gaacacgttc
     2161 tgcccgagta tcttgaaaag ctgggcgttg agtacaagat tgttgaagag aatacttacg
     2221 gtatcgtgaa agagaagatt ccagagggca aaaccacttg ctcactgtgt tctcgccttc
     2281 gtcgcggtat cctttatcgt accgcaacgg aactgggggc gacgaagatc gcgttgggtc
     2341 accatcgtga cgatatcctg caaacgttgt tcttaaatat gttctacggc ggcaagatga
     2401 aaggtatgcc tccgaaactg atgagcgatg atggcaaaca tatcgttatt cgtccgctgg
     2461 cctactgccg cgagaaagat attcagcgat ttgccgatgc aaaagcgttc ccgattattc
     2521 cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca ggtgattgct gacatgttgc
     2581 gtgactggga taaacgttat ccagggcgta tcgagacgat gttcagcgcg atgcagaatg
     2641 tggtgccgtc gcatctgtgc gataccaacc tgttcgattt caaaggcatt acccacggtt
     2701 ctgaagtggt taacgggggt gatctggcgt ttgatcgcga agagatccca ctacaaccgg
     2761 cgggctggca gccagaagaa gatgaaaatc agttggatga gttacggctg aatgtggttg
     2821 aagtgaaata accaggatag cgcccgatgc gcaagcgtat cgggctactc ttatggaggc
     2881 cggataagac gcggccagcg tcgcatccgg caatcccgca taagatgttt actcttgcac
     2941 ccggcaattc aacatttcat tattttaata accgcacccg gcacgttttt cctttaatct
     3001 tcccgccctg taactgtttc catgctttat gagcaacagc ctgacggacc gcgacataga
     3061 catgcgccgg atgcacggcg attttgccaa tatctacgcc atcaagcccg atgtctcctg
     3121 tcagcgcacc taatacatca cccgggcgca ttttggcttt tttcccgcca tcgatacaca
     3181 acgttgccat ttctgcttcc agcggcaaaa tggaactatt agctggcggc gtttgccagt
     3241 taagttttat ctgcaacatg tcagaaatga tattggcccg ctgtgcttct tccggagcac
     3301 agaaactgat cgccagaccg ctatttcctg cacgagctgt acgaccgatg cgatgtacat
     3361 gaacttcagg gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat ttaatatcca
     3421 gaccacgcgc agcaacatca gtcgcaacca gtacacgggc gctaccgtta gcaaaacgta
     3481 ccagggtctg atcgcgatcg cgttgctcca aatcgccgtg taatgacaat gcactttgcc
     3541 ctacttcatt cagcgcgtcg cagacagcct ggcaatcttt tttggtattg caaaacacca
     3601 cgcaagagga tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttgc
     3661 tggatgtctc ataaaattgt tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa
     3721 tcgccaaagg atcgcgttgc actcgtccgc tgattacggc gatggcttcc ggccaggttg
     3781 ccgaaaacag aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca tcaatggcat
     3841 cgctaaatcc catatccagc atgcggtcgg cctcatccat caccaaagta ttcagcgcat
     3901 ccagtgatac cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt gccacgataa
     3961 tatgcggcgc atgttgcaac gaatcacgct gcataccgaa cggttgacca ccgcacaacg
     4021 tcaaaatttt ggtatttggc agaaaacgcg ccagccgacg caattcacct gccacctgat
     4081 ccgccagttc acgcgtagga cacagcacta aagcctgggt ttgaaatagc gacgcatcaa
     4141 tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca
     4201 cgcgaacatc ttttccggca aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatag
     4261 ttaaataacc caactcatta aggttcgtga gttgggcggg aggcaaaaca ttcagggtag
     4321 aaaaagcggt cacaatctat tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct
     4381 cgcagatcta cgctcacgat gcgacaattt aatcggttct tcatcaggtg gtgggtcagg
     4441 catgggttgc gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc
     4501 agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga
     4561 ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg
     4621 cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag
     4681 cgcaacgccc atcgcttgac gttgcattca cctgcaaggg agatattgcc ctgaatgggt
     4741 agagagttta ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccggaaaa
     4801 attgcttttg ttacaaccat ttactacggt gcaaccataa agcaacacca ccaataagaa
     4861 caactaacag aatacaaaaa attgaaaatc cgaattgcca cccgccgcca gggatcccac
     4921 caaggttgac gccaaataac ccggtcagaa aggtactggg taaaaagacc attgccatca
     4981 acgacattgt ataggtacga cgagctaaat tttcctgcat cacctgagcg atttcatccg
     5041 ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat
     5101 cggcaatatc ctgcatccga cggcgttggt catcgctcat ccacggcaaa cgttcactgg
     5161 caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt
     5221 tgcgcagcag agccaggaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa
     5281 ggtcgataat tttatcgtgc agctgctcga taaattcgct ggaatgatcg gtcaacgcat
     5341 cgcacacatc caccagccat cccccgcaat cggtcggacc cgtgccctct tccagatcgc
     5401 tcaccacatc gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt
     5461 ccatatatac acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac
     5521 agcgcaatgt aatcagcgtg ccttcaccga gacggctgac tcggggccgt gtgctctcgc
     5581 ccgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc
     5641 tatcatggtg tacataatta aggtggagcc agcagggatg cgcttcatca atcacatctg
     5701 tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat actgcatccg
     5761 ggacattaac gtccgatccc ttaatcgctt ccacagtgcc tccatcatca acgcattatt
     5821 ttgtagtcta gccttctggc cctgttacgc aacatctcat ccccccatta ccctgaaatg
     5881 attaataaaa ttctgtctaa attgaataca aaaagcaaaa tgcttttccg tatacaaacc
     5941 gtgtgaagtg ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt
     6001 ttttgcgatc ggatagcaac aaaaattgat aaaaataacg ggatctcaat gattacgcac
     6061 aacttcaata ccctggactt actcaccagt cctgtctgga ttgtttcgcc ctttgaggaa
     6121 cagttaattt atgccaatag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag
     6181 ctacgaaccg gaccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat
     6241 ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca
     6301 gcattaagct gtcggcttgt tttgtgagag ctgacagaaa cagaaccggt gattattttc
     6361 gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag
     6421 cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg
     6481 attgacccct cacaagatgg ccaaatcgtc gatgctaacc tcgccgcgct caatttctat
     6541 ggttataacc atgaaacgat gtgccagaaa catacctggg aaattaatat gctcgggcgt
     6601 cgcgtcatgc ctatcatgca tgaaatttcg catttacccg gcggtcataa acctttgaat
     6661 tttgttcata aactggcgga tggatctact cgtcatgtgc agacctatgc cgggccgatt
     6721 gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg
     6781 ctggaggagc agctggaaca tgctgctcac catgacgcga tgaccggatt actgaatcgg
     6841 cgacagtttt atcacattac ggaaccaggc caaatgcagc acctcgccat cgcccaggat
     6901 tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat
     6961 tctaaaggtg atgaggtgtt atgcgccctc gcccgcaccc tcgaaagttg cgctcgcaaa
     7021 ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca
     7081 ctggataccg cgctttcgct ggctgaaact atccgtgtaa gcgtggcaaa agtgagtatt
     7141 tcgggcttac cacgttttac cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc
     7201 atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc
     7261 aaccgcgtgc tggcggcata agccgcggat gcgtctcgag atcaacggct gcgcttagcg
     7321 tggcgctccc agttttcttg cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc
     7381 ccgctgccgc catgatgcgg tagcgcggtg caatatgcct gaacatcatc aaattcggtc
     7441 agccagcgcg ccacatagct gcggacaata ttggcatgcg atttatcatc ccgtcctttg
     7501 ccatgaataa tcagcacgtt acgcaaacca tcagccatgg cttgttgaat gaaactgaac
     7561 atcattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga
     7621 tatttaccac tgcgcagctt atccagcacc ccatgttgca acccttcccg ccgaaactcc
     7681 agcggctgac ttagtgggat gatgtcgaga aatccggtgg tgaggaaatt atcaagctgc
     7741 agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatcggta
     7801 gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg
     7861 ttcatgttac atcctcccgc aattaagagc gcgatatgat aaccagaccg ggtcggtcca
     7921 acaacgtatt acccaaaatt ccagtaataa gttccaaata ttgccgatat tttaagcaaa
     7981 atgcttatgc atgattattc attcacgata ttaataatgt aacttatatt ttcgtgaaat
     8041 ctgtcactga agaaaattgg caactaaagg ttaaaaccgt tataacacag tcaccggcgc
     8101 agaggagaca atgccggatt taagacgcgg atgcactgct gtgtgtactg tagagtctgg
     8161 cggatgtcga cagactctat ttttttatgc agttttaact ttgcagatag ccgcattctc
     8221 gccggaattg cgtgattaat gacgcggtca gtggtgtctg gcgactatca cgccgctgaa
     8281 tcaaataata ggccgctttc ggtaaaattt cgctaaccgg caacatcacc agcccctgtc
     8341 cgtgcaaggg atcgcagccc atttcttcag gcagtatgct gagaaaatcg ctttttgcca
     8401 ccagactgat acaggctgag aacgtctcgc agactacacc gacctgtggc gtttgcgcct
     8461 gatcgtcaag caattcactc aactgtttgt agtaactgcc gtgtggcgtc ggcattgtcc
     8521 agctgtaatc cagtaactgt ttgatcgaac gggcaccaat ggcggggtgt cccgggcggc
     8581 aaaagatcgc gaattgcttt tccagtaatt tctcaaaagt aaattcgtgg tcgtacggtc
     8641 cctgataata ggtattgatg gtgaaatcca attctccctg acgcaattca ttaatcatcg
     8701 acaccagttg cccttccata atgcgtactt ttacctgcgg atgctgctga tgaaaacgag
     8761 atatgacagc tggcatcaga ctgcgggaaa tactggcccc catgccgata ttaatctgcc
     8821 ctgccagttg cccttgtcgt tggcgaatat cctcttgggc tgcgcgcagc tcttcaagaa
     8881 taagactggc gtgctgataa aaactttcac cggcatcagt taacgtcacg cctttactac
     8941 ggcgaaaaaa gagttgcgcc gctaatcctt cttccagctc ctgaatagat ttactcagtg
     9001 ccggttgcga catattcaac attcggctcg ctccgcgaat gctgccctga cgagccactt
     9061 caacaaaagc acgaatttga tgaattttta cctgaaaagc catgacgcca ccgataaccg
     9121 ttatttatca gaccaaagaa actggcatct actttaatgc agatgattgt gtcagggtaa
     9181 tttatgaacg gttaaaactg tgaaaaatca gttagtgata agtaaaaact atcgctacgt
     9241 gaaccgggtc acacttttta ctgatgacgg gaaaggttat ggagtctttg aatcaatttg
     9301 ttaattcgct tgccccaaaa ttatcgcact ggcgacgtga ttttcatcac tatgcagagt
     9361 ctggctgggt ggaattccgc actgccaccc ttgttgcgga agaattgcag cagctcggct
     9421 attcactggc gctgggccgc gaagtagtta atgaaagtag ccggatggga ctacctgatg
     9481 aactcactct acaacgcgaa ttcgagcgcg ctcgtcaaca gggtgcgcta gcacaatgga
     9541 ttgcggtttt tgaaggtggt ttcactggta tcgtcgccac cctggatacc ggtcgccccg
     9601 gtccggtgat ggctttccgt gtcgatatgg acgcgctgga tctcagtgaa gagcaggatg
     9661 tcagccatcg cccctaccgc gacggttttg cgtcatgtaa cgccggaatg atgcatgcct
     9721 gtggtcatga tggacatacc gccattgggc ttgggctggc gcataccctt aaacaattcg
     9781 agtccggact acatggcgtc atcaaactga tttttcagcc tgcagaggaa ggtacgcgtg
     9841 gcgcgcgggc gatggtcgat gcaggtgtcg tagatgatgt tgattatttt actgccgtgc
     9901 acattggcac tggcgtacct gcgggcaccg tggtgtgcgg cagtgataat tttatggcaa
     9961 ccaccaaatt tgacgcgcac ttcaccggta ccgccgctca cgcaggcgca aaaccagaag
    10021 acggtcacaa tgccttgttg gcggcagcac aagccactct tgcactgcat gcaatcgccc
    10081 cgcacagcga aggagcttcc agagtaaacg tgggcgttat gcaggcagga agcggtcgta
    10141 acgttgttcc tgcctcggcg ttgctgaaag tggaaacacg cggggccagc gacgttatta
    10201 atcaatatgt ttttgaccgt gcacaacaag cgattcaggg cgcagcaacc atgtatggtg
    10261 tcggcgttga aactcgtctg atgggtgcag ctaccgccag ttctccttcg ccgcaatggg
    10321 tcgcatggtt gcaaagtcag gcggctcagg tcgcgggggt caatcaggcc attgaacgtg
    10381 ttgaagcgcc tgcgggttcc gaagatgcca cattaatgat ggcccgcgtg cagcaacatc
    10441 aagggcaagc ctcctacgtg gtgtttggca cacagctggc ggcaggtcat cacaacgaaa
    10501 aattcgattt tgacgagcag gttctcgcta ttgccgtcga aacgctggcg cgcaccgcgc
    10561 tcaattttcc ctggacgcga ggtatctgat gcaggaaatc tatcgtttta tcgacgatgc
    10621 gattgaagcc gatcgccaac gttataccga tattgccgat caaatctggg atcatccaga
    10681 aacacgtttt gaagagttct ggtcagcgga gcatctggct tcggcgctgg aatctgcagg
    10741 cttcaccgtt acccgcaacg taggcaatat cccaaatgcc tttattgctt cgtttggtca
    10801 aggcaaaccg gttatcgccc tgctgggaga atatgacgcc ctggcaggtt taagtcagca
    10861 agctggttgc gcgcaaccta catccgcgac gcccggtgaa aatggtcacg gttgcggaca
    10921 caatttgctg ggaaccgccg cctttgccgc tgcaatagcc gtcaagaaat ggctggaaca
    10981 atatgggcaa ggcggcacag tgcgctttta tggttgtcct ggcgaagaag gcggctcggg
    11041 taaaacgttc atggtccgcg agggggtatt tgatgatgtg gatgcggcac tcacctggca
    11101 cccggaagcc tttgccggta tgttcaatac ccgcacgctg gcaaacattc aggcatcatg
    11161 gcgctttaaa gggatcgcag cacatgccgc gaattcccct catttgggac gcagcgccct
    11221 tgatgccgta acgttgatga ccactggcac caacttcctc aacgaacata ttattgaaaa
    11281 agcgcgcgta cactatgcca tcacaaatag cggcgggatc tcgcccaacg tggtccaggc
    11341 gcaggcagaa gtgctttatc ttatccgcgc ccccgaaatg accgacgtgc agcatattta
    11401 tgatcgggtc gccaaaatcg ccgaaggtgc ggcattgatg accgaaacca cggttgaatg
    11461 ccgcttcgac aaagcctgtt ccagttatct cccgaatcgc accttagaaa atgccatgta
    11521 ccatgcccta tcccattttg gtaccccgga atggaactcc gaagaactgg cttttgcgaa
    11581 acaaattcag gctacgctca cccccaacga tcggcaaaac agtctgaata atatcgccgc
    11641 aaccggtggc gaaaacggca aggcttttgc actacgtcat cgtgaaacgg tactggcgaa
    11701 tgaggtcgct ccatatgccg ccaccgataa cgtgcttgcg gcatcgactg atgtcggcga
    11761 cgtcagttgg aaactgcctg ttgcccagtg tttcagcccc tgttttgccg tcggtacacc
    11821 gctacatacg tggcaactgg ttagccaggg gcgaacatct attgctcata aaggaatgct
    11881 gctggcggcg aaaactatgg cagcaaccac agtcaatctc ttcattgatt cagggctatt
    11941 gcaagaatgc caacaagagc atcagcaagt tacggacacg caaccgtatc actgccctat
    12001 cccgaaaaac gtgacaccgt cacctttaaa ataacaacaa caacgcaaac acaacaaccg
    12061 aggaatgccc atgagtatgt catccatacc gtcgtcctcc caatccggga agctctatgg
    12121 ctgggtcgaa agaattggta acaaggttcc ccatcctttt ttgctcttta tctatttgat
    12181 aatcgtactc atggtgacga cggcaatttt gtcagccttt ggcgtcagtg cgaaaaaccc
    12241 gaccgatggt acgccggtgg tggtgaaaaa cctgctcagt gtagaaggat tacactggtt
    12301 tttacccaat gtgattaaaa actttagcgg ttttgctcca cttggagcga tcctggcgct
    12361 ggttttaggt gccggcctag cggagcgcgt cggcttactg ccagcgctaa tggttaaaat
    12421 ggcatcgcat gttaatgccc gctacgccag ttatatggtg ctgtttattg cttttttcag
    12481 ccacatttct tccgatgcgg cgttagtgat catgccaccg atgggtgcgc tgatttttct
    12541 ggcggtgggc aggcatccag ttgcaggttt actggctgcc attgcaggcg taggttgcgg
    12601 ctttacggct aatttactga ttgtcacaac cgacgtgttg ctgtcgggga tcagcacgga
    12661 agcggcggct gcgttcaatc cgcaaatgca cgtcagtgta attgataact ggtattttat
    12721 ggccagctcc gtagtcgtac tgacgattgt tggcggcttg ataaccgaca aaatcatcga
    12781 gccacggtta ggtcaatggc agggaaacag cgatgagaaa ctgcagacat tgaccgaaag
    12841 tcagcgtttt ggtttacgca tagcaggtgt cgtatcgcta ctttttattg ctgcgatcgc
    12901 gctgatggtg atcccggaaa acgggatatt gcgcgatccg attaatcaca ccgtgatgcc
    12961 atcacccttt attaaaggta tcgtgccact aatcattctt tttttctttg tggtttcgct
    13021 ggcttatggc atcgctaccc gcacaattcg acgtcacgcg gatttaccgc atttaatgat
    13081 tgaaccgatg aaagagatgg cgggatttat cgtgatggtt tttcccctcg cccagtttgt
    13141 cgccatgttt aactggagca acatggggaa attcatcgcc gtggggctga ccgatatact
    13201 tgaaagttca gggcttagcg gcatcccggc gtttgtcggt ctggcgttgc tttcctcttt
    13261 cttatgcatg tttattgcca gcggttccgc aatctggtcg attctggccc ccattttcgt
    13321 accaatgttt atgctacttg gctttcaccc ggcatttgcg caaatcctct ttcgtattgc
    13381 cgactcatcc gtattgcctt tagcgccggt atctcctttt gttccactgt ttcttggatt
    13441 cctgcaacgc tacaaaccag acgcgaaact gggtacttac tattcgttag tcttgcccta
    13501 tccgcttatc tttttggtgg tatggctgct gatgttgctg gcgtggtatc ttgttggtct
    13561 gccgataggt ccggggattt acccacgttt gtcttaagag agaacggatg ctgagattac
    13621 ttgaagaaaa aattgccacg ccactgggtc cactgtgggt gatttgcgat gagcaatttc
    13681 gcctgcgggc ggttgaatgg gaagagtaca gcgaacgcat ggtgcagctg ctggacatcc
    13741 attatcgcaa agaaggctat gagcgcattt ctgccaccaa cccaggcggt ttaagcgaca
    13801 agcttcgtga ttattttgcc ggtaatctta gcattattga tacgcttccc actgccacag
    13861 gggggacgcc atttcagcgc gaagtctgga aaactctacg cactatcccc tgcgggcagg
    13921 taatgcatta cggccaactg gctgaacaat tgggccgccc tggcgcggcg cgtgccgttg
    13981 gtgcggcaaa tggatcgaat cccatcagca tcgtcgtacc ttgccatcgg gttattggcc
    14041 gaaacggcac catgaccgga tatgcaggcg gagttcagcg aaaagagtgg ttattgcgcc
    14101 acgaaggtta tcttttgctg taaacattaa acaatttgtg ccagcttgtt cacactttta
    14161 tgtaaagtta cccttaacaa ctta
//
LOCUS       DAJSKJ010000101        14140 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000101 DAJSKJ010000000
VERSION     DAJSKJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.101"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            167..1225
                     /gene="citC"
                     /locus_tag="ON345_002543"
     CDS             167..1225
                     /gene="citC"
                     /locus_tag="ON345_002543"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR3072590.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1240..1536
                     /gene="citD"
                     /locus_tag="ON345_002544"
     CDS             1240..1536
                     /gene="citD"
                     /locus_tag="ON345_002544"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR3072591.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSGEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1533..2441
                     /gene="citE"
                     /locus_tag="ON345_002545"
     CDS             1533..2441
                     /gene="citE"
                     /locus_tag="ON345_002545"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR3072592.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2452..3984
                     /gene="citF"
                     /locus_tag="ON345_002546"
     CDS             2452..3984
                     /gene="citF"
                     /locus_tag="ON345_002546"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR3072593.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCIPPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3988..4539
                     /gene="citX"
                     /locus_tag="ON345_002547"
     CDS             3988..4539
                     /gene="citX"
                     /locus_tag="ON345_002547"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR3072594.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4514..5392
                     /gene="citG"
                     /locus_tag="ON345_002548"
     CDS             4514..5392
                     /gene="citG"
                     /locus_tag="ON345_002548"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR3072595.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVLNHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5443..6906
                     /gene="citT"
                     /locus_tag="ON345_002549"
     CDS             5443..6906
                     /gene="citT"
                     /locus_tag="ON345_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR3072596.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7020..7826
                     /gene="rna"
                     /locus_tag="ON345_002550"
     CDS             7020..7826
                     /gene="rna"
                     /locus_tag="ON345_002550"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR3072597.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8056..8466
                     /gene="rnk"
                     /locus_tag="ON345_002551"
     CDS             8056..8466
                     /gene="rnk"
                     /locus_tag="ON345_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR3072598.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8697..9935)
                     /locus_tag="ON345_002552"
     CDS             complement(8697..9935)
                     /locus_tag="ON345_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR3072599.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLSIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10156..10584
                     /gene="uspG"
                     /locus_tag="ON345_002553"
     CDS             10156..10584
                     /gene="uspG"
                     /locus_tag="ON345_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR3072600.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10705..12270)
                     /gene="ahpF"
                     /locus_tag="ON345_002554"
     CDS             complement(10705..12270)
                     /gene="ahpF"
                     /locus_tag="ON345_002554"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR3072601.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12441..13004)
                     /gene="ahpC"
                     /locus_tag="ON345_002555"
     CDS             complement(12441..13004)
                     /gene="ahpC"
                     /locus_tag="ON345_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR3072602.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13376..14122
                     /gene="dsbG"
                     /locus_tag="ON345_002556"
     CDS             13376..14122
                     /gene="dsbG"
                     /locus_tag="ON345_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR3072603.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3348 a         3749 c         3575 g         3468 t
ORIGIN      
        1 aatttcacgc cgaaatactc cttaggatgt atagcgaaag gagaaaaaga tatacctcga
       61 tcacccccct ttctcccaag tgaaaataaa aggttatcag tttgcaacat tgaacaacat
      121 tcgttgcaaa tcgataacaa catgcacctt caggatacta tttattatgt tcggcaatga
      181 tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct
      241 gcatgaaaat gatttgagcg ttgacaccac agtcgaagta tttattaccg taacccgcga
      301 tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt attaaatgcg ttgctatcag
      361 tgaatccgtt cgcggtgaag gactggcgct gacattagcc actgaactga taaacctcgc
      421 ctatgagcgg cacagcacgc atctgtttat ttataccaaa accgaatacg aggcgctgtt
      481 ccgccagtgc ggtttttcca cgctgaccag cgtacccggc gtgatggtgc tgatggaaaa
      541 cagcgccacg cgactgaaac gctatgccga atcgctgaaa aaatttcgtc atccagggaa
      601 caagattggc tgcattgtga tgaacgccaa tccctttacg aatggtcacc gttatctgat
      661 tcaacaagct gcggcacagt gcgactggtt gcatctgttt ttagtcaaag aagattcttc
      721 acgcttcccc tatgaagacc ggctggatct ggtgttaaaa ggcaccgccg atattccacg
      781 cctgactgtg catcgcggct ccgaatacat catctcccgc gctacgttcc cttgctactt
      841 cattaaagaa cagagcgtca ttaaccattg ttacaccgaa attgatctga agattttccg
      901 tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt gtcggtactg aacccttttg
      961 tcgcgttacc gcccagtaca accaggatat gcgctactgg ctggaaacgc cgactatctc
     1021 cgcaccgccc atcgaactgg ttgaaattga gcggctgcgt taccaggaga tgccgatatc
     1081 cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc acggctatcg cgccgctggt
     1141 ccctgcagtc acgctgcatt atttgcagaa cctgcttgag cactcccgcc aggacgcggc
     1201 agctcgtcaa aagacccccg catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc
     1261 gttgcaggca cccttgagtc tggggatgtg atgatacgca tcgccccact cgatacgcag
     1321 gatatcgacc tgcaaatcaa tagcagcggt gagaaacagt ttggcgatgc aattcgcacc
     1381 accattctgg acgttctcgc ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac
     1441 aaaggcgcac tggactgcat tttacgtgca cgactggaag ccctgctggc acgcgccagc
     1501 ggtatcccgg ctctgccatg ggaggattgc caatgatttc cgcttcgctg caacaacgta
     1561 aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc caatgccgcg atggtcagca
     1621 actccttcat ctacccggct gatgccctga tgtttgacct cgaagactcc gtggcattgc
     1681 gtgaaaaaga caccgcccgc cgcatggttt accacgcgct gcaacatccg ctgtatcgcg
     1741 atattgaaac cattgtgcgt gtcaacgcgc tggattccga atggggtgtc aacgacctgg
     1801 aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg
     1861 atgttctgga tatcgaaaaa gagatcctgc gtatcgaaaa agcctgtggt cgtgaacccg
     1921 gcagcaccgg cctgctggcg gcgattgaat ctccgctggg cattacccgc gcagtggaaa
     1981 tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca
     2041 acctgcgtac agaacgctcc ccggaaggaa ctgaactgct gttcgcacgc tgttccattt
     2101 tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac cgtctattcc gacgctaaca
     2161 acgaagccgg attcctgcaa gaagccgccc acatcaaaca gctgggcttt gacggcaaat
     2221 cgctgatcaa cccgcgtcag attgatctgt tgcacaacct ctacgcacca acccagaaag
     2281 aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg
     2341 gcgtggtttc cctgaacggc aagatggtgg acggtccggt tatcgatcgc gcccgtctgg
     2401 tgctctctcg tgcagaactt tccggcatcc gcgaagaata aggcaatcaa aatgacgcag
     2461 aaaattgaac aatctcaacg acaagaacgg gtagcggcct ggaatcgtcg cgctgaatgc
     2521 gatcttgccg ctttccagaa ctcgccaaag caaacctacc aggctgaaaa agcgcgcgat
     2581 cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct ctggtttaca ggacggcatg
     2641 acggtttcct tccatcacgc tttccgtggc ggtgacctga ccgtcaatat ggtgatggac
     2701 gtcatcgcga agatgggctt taaaaacctg accctggcgt ccagctccct gagtgattgc
     2761 catgcgccgt tggtagaaca cattcgccag ggcgtggtta cccgcattta tacctccggc
     2821 ctgcgcggtc cactggcgga agagatctcc cgtggtctgc tggcagaacc ggtgcaaatc
     2881 cactctcacg gcggtcgcgt gcatctggta cagagcggcg aactgaatat cgacgtggct
     2941 ttcctcggtg tcccgtcctg cgatgaattc ggtaatgcca acggctacac cggtaaagcc
     3001 tgctgcggct ccctcggcta tgcaatggtt gatgccgaca acgcaaagca ggtcgtgatg
     3061 cttaccgaag aactgctgcc ttatccgcat aatccggcaa gcattgagca agatcaggtt
     3121 gatttgatcg tcaaagttga ccgcgttggc gatgctgcaa aaatcggcgc tggcgcgacc
     3181 cgtatgacca ctaacccgcg cgaactgctt attgcccgta gcgctgcgga tgtgattgtc
     3241 aactcaggct acttcaaaga aggtttctcc atgcaaaccg gcaccggcgg cgcatcgctg
     3301 gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc
     3361 cttggcggta ttaccgcgac gatggttgac ctgcacgaaa aaggtctgat ccgcaaactg
     3421 ctggatgtgc agagctttga cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac
     3481 atcgaaatca gcgccaacca gtacgctaac tggggttcga aaggcgcatc ggttgatcgt
     3541 ctcgacgtgg tggtactgag cgcgctggaa attgacaccc agttcaacgt taacgtgctg
     3601 accggctctg acggcgtact gcgtggtgct tccggtggtc actgcgatac cgcgattgcc
     3661 tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc gtattccgac tctggtggat
     3721 aacgtgctga cctgcatccc cccgggctcc agtgtcgata ttctggtcac agaccacggt
     3781 atcgcagtta acccggcacg tccggaactg gcagaacgtc tgcaggaagc gggcattaaa
     3841 gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg
     3901 attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat
     3961 gttgtgcatc aggtgaagga ataagccatg cacctgcttc ctgaactcgc cagccaccat
     4021 gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgtc
     4081 tggctcaagc gccatcctgt tccactggtc tcctttaccg tggttgcgcc tgggccgatt
     4141 aaagacagcg aggtcacacg ccgaattttt aatcatggcg tgacagcctt gcgtgcctta
     4201 gccgcaaaac agggctggca aattcaggag caggctgcac tggtttccgc cagcgggccg
     4261 gagggcatgt tgagcattgc cgccccggct cgcgacctca agctcgccac cattgagctt
     4321 gaacatagtc atcctctcgg gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa
     4381 attctctccc gccgcgacta ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc
     4441 gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg atttactcaa ccgcatggag
     4501 gcactgctga acgatgtcga tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc
     4561 attaatcgat gagtacgccc tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc
     4621 accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg cacaaagata tggcgctgga
     4681 agatttccac cgcagcgcgc tggcgattca gggctggtta ccccgtttca ttgaatttgg
     4741 tgcctgtagt gcagaaatgg caccggaagc ggtactccac ggattacgcc caattggtat
     4801 ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta aacacgcata aaggcagcat
     4861 tttttcttta gggctgctat gtgcggcaat tggccgtttg cttcagctca accagccggt
     4921 aacgccaaca accgtttgtt ctacggcggc aagtttctgc cgtggtctga ccgatcgcga
     4981 actgcgtacc aataattcac aactgacggc aggtcaacgg ttgtaccagc agcttggcct
     5041 taccggcgca cgcggtgaag ccgaagcggg ttatccacta gtgctcaacc acgccctgcc
     5101 gcattacctc actctgctgg atcaggggtt agatcctgaa ctggcattgc tcgatacctt
     5161 gctcctactg atggcgatca acggcgatac caacgttgcg tcgcgcggtg gcgagggggg
     5221 cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa aaagggggca ttcgaacccc
     5281 cgccgatctc gattatctcc ggcagttcga cagggagtgt atcgaacgaa atctcagtcc
     5341 aggcggcagt gccgacctac tgatccttac ctggttttta gcacagattt aattatttaa
     5401 gcacttgata aatttggaaa tattaatttt cggagaaccc gtatgtcttt agcaaaagat
     5461 aatatatgga aactattggc cccactggtg gtgatgggtg tcatgtttct tatccctgtc
     5521 cccgacggta tgccgccgca ggcatggcat tacttcgctg tgtttgtggc aatgattgtc
     5581 ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt ttattgcggt tactatttgc
     5641 gttattggca gtaattacct gctctttgat gccaaagaat tagctgaccc agcgtttaat
     5701 gcgcaaaaac aggcgctgaa atggggtctg gctggttttt ccagcaccac ggtatggctg
     5761 gtatttggcg catttatttt tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt
     5821 gcccttttcc tggtgaaatt catgggcaaa cgcacgctga cgttgggtta tgcgattgtc
     5881 attatcgaca ttctgctggc accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg
     5941 gtttttccgg tcattaaaaa cctgccgccg ctgtttaaat cattccccaa cgatccgtcc
     6001 gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca ttagtaccag tctgagttcg
     6061 tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc tggagttcgt cagcaaaatt
     6121 gccggtatcc agattagctg gttgcagtgg ttcctctgct tcctgccggt tggggttatc
     6181 ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca aaccggaaat cacacacagt
     6241 gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga
     6301 gagtggacgc tgattggcct tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa
     6361 gtcattaatg ctactgcggt tggtctgctg gcagtttcgc taatgctggc tctgcacgtt
     6421 gtgccgtgga aagacattac ccgctataac agcgcatgga acacgctggt caacctggca
     6481 actctggttg tgatggctaa cggcctgact cgttctggtt ttattgactg gttcgccggt
     6541 accatgagta cgcacctgga aggattctca ccaaacgcaa cggtgattgt actggttctg
     6601 gtgttctact ttgcacacta cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg
     6661 ccggttattc tggccgtcgg taaaggtatt ccgggcgtac caatggaaca actgtgtatc
     6721 ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg
     6781 gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc
     6841 ttcggggtga tttacatctc tatgttgctg ttggttggct ggccgattct cgccatgtgg
     6901 aactaatacg ttcgccaaac gctgaataaa tcatcacgcc cgccaggtaa gcccacctgg
     6961 cgggcttttt tatgatttaa tagatagtat tcagtgtatg tatgagttcc acacccatta
     7021 tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc tctgcttccc ttctcttctg
     7081 ccaacgcctt agcgttgcag gcaaaacagt atggcgattt tgatcgctat gtcctggccc
     7141 tctcctggca aaccggattt tgccagagtc aacacgatcg aaatcgtaac gaacgagatg
     7201 aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt tctgaccgta catggtctgt
     7261 ggccaggatt gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc tggatgcgct
     7321 tcggttgcgc tactcgccca atcccgaatc taccagaagc gcgcgccagc cgaatgtgtt
     7381 catcgccgga aaccggatta tcactggaaa cggccgctaa actaagtgaa gtcatgccag
     7441 gagctggcgg acgttcctgc ctggaacgct acgaatatgc caaacacggt gcctgctttg
     7501 gttttgatcc ggacgcatac ttcggtacga tggtacgcct gaatcaagaa attaaagaga
     7561 gcgaagccgg aaaattcctt gcggataatt acggtaaaac agtgagccgc cgtgactttg
     7621 acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc
     7681 agggtaaccc tgcgtatttg actgaaattc agatctcgat caaagctgac gccatcaacg
     7741 ctccgctttc tgcaaactca ttcttgccac aaccacaccc aggcaactgt ggcaaaacct
     7801 ttgtgattga taaagtgggt tattaatcat ctgatttaca aaacacgcta attcgtcttc
     7861 cataaaccgt gacgattaag aattatacat aatgcgctgc attgaggaat tccctgaatg
     7921 tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag ttaaaccctc
     7981 gccgcctgac ggtgagggtt ttcttttggg tatgtttatc tgcgacactc gcagtaccga
     8041 cgacatggag taaaaatgtc cagaccaact atcatcatta acgacctgga tgccgaacgc
     8101 atcgatattc tgctggagca acccgcctat gctggtttgc caatcgccga cgcgttaaac
     8161 gcagagttgg atcgcgccca aatgtgttcg ccagaagaga tgccacacga cgtggtgaca
     8221 atgaacagcc gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg
     8281 gtgtatccgg caaaaatgac cgatagcaat actcagcttt ccgttatggc tccggtaggt
     8341 gccgcactgc tggggctgcg cgttggcgat tccattcact gggaacttcc gggcggcgtt
     8401 gcaacccacc ttgaagtgct ggaactcgaa taccagccag aagctgctgg cgactacctg
     8461 ctttaatccc atctgcacaa gcgataactc gctttgccca gaggatgcac tcgcatcctc
     8521 tttacgcaaa atcagcaatt tttcctggtt ctcactgttc acttttgctg caaaattatt
     8581 ccgtttcgtt tgtgggaata accatggcag aagcatttta catcctgata ggatttctga
     8641 ttatggcggc gattatcgtc atggctgtcc tttacctcga aaaccactcc tgacgatcat
     8701 attgttcccc ccggcatcgc attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg
     8761 ctttgtgcac cgggtaccag aatcacctta cggcactcct cttcacgttt ttcgaaaatc
     8821 tcatatcccc gggcggcctc ttcaaacggc atatagtggg taacaatttc ttctggtttc
     8881 agtaatcctt tctcaattaa cggtaataat tctcccagcc atgcgtgaac gtgggtctgt
     8941 cccattttaa acgtcaaccc tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat
     9001 ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc tgaccgccgc aatacactgg
     9061 cgcaacgctt taccgctgct gccctccagt ttcaggttag tcagcaccgt ttccgtggtg
     9121 ctgcctttcg cttcaaaacc gacggcgtct attactgcat ccacgccccg gtgacctgcc
     9181 gtttgttcaa taattgactg tgccggatcg ctatcttcat cgaaattaat cgggatcgcg
     9241 ccgtagcggt cagcggcgaa acgcaagcgg tagggatgat gatcaacaac aaaaatctgt
     9301 tccgcaccga gcaaccgtgc acaggcgatt gacaacaatc ccactggacc ggcaccatag
     9361 actgcaacgc ttgaaccttg ttggatctgc gcattttttg ctgcctgcca tgccgttggc
     9421 agaatatcag aaaggaaaag ggctttatca tctgaaagca aaggcggtac tttaaacggc
     9481 cccacattcc ctttagggac gcggacatat tccgcctgcc caccaggaac gccgccatac
     9541 aggtgactat aaccaaacaa tgccgcggga gctggtatct gttttttatt gagcgcagcg
     9601 cctttacccg cattggtatt ttcgcaggcg gcatattgct gtaatcgaca gaaaaaacag
     9661 tcgccacaag caatgacaaa cggaattacc acccggtcgc ctttttgcaa attttttacg
     9721 tcctttccgg tttcaacgac ttcccccata aattcatgac caaaaatatc gccatgttta
     9781 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttgccgta
     9841 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
     9901 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagaggc agttcaaaaa
     9961 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    10021 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    10081 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttat tcatgattaa
    10141 cagggagaaa gggttatgta taagacaatc attatgccag ttgatgtatt tgaaatggaa
    10201 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    10261 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    10321 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    10381 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    10441 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttattgg ttctcgcaac
    10501 ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg ccatgccaat
    10561 ctgccggtgc tggttgtgcg ttaattgcca gaataaatat cccgccctgc ccggagccat
    10621 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    10681 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    10741 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    10801 cgtacagtca cccgctgcga atacgccttt gacgttggtt tcgcatttcg catcaatgat
    10861 aatctcaccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcggcagcag
    10921 accaatctgg acgaaaatac cggccagttc gatgttgtga atatcgccgc tgacgcgatc
    10981 acgatattcc agacctacga ctttgctacc gtcgcctttc acttccgtgg tttgtgcatt
    11041 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt
    11101 catttctggc gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcaattgc
    11161 cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttacctt taaacagcgg
    11221 gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga tactgatctt cgcccggaac
    11281 gttcatgttg cgccattttg caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc
    11341 gccagaagct gtttcaatct gatgcagacc accttcaact gctgctggga tcagtttgct
    11401 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    11461 tgccagcttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    11521 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    11581 gtaaattgct gccgctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc
    11641 acgcttgttc agctcttctg ccgcacgttt ttccgcgccc gtatcaattt tggcaacgat
    11701 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    11761 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    11821 cttgatgcgc gggttcagta cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    11881 atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    11941 ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac
    12001 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggcccct ggttggaacc
    12061 tgggttggtg atcaggaaag acggcttacg caccggcaag ctgttatctt ctttaaaggt
    12121 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    12181 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    12241 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    12301 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    12361 ggtgccgcag cacccgaaga attaggtgaa tttccgaagt atttcgcgcc ggagttggta
    12421 gcgcgaaaga cgaaggaaat ttagatttta ccaaccaagt ccagagacgg agccagagtt
    12481 gcttcacctt ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactgt
    12541 gctgctttga ttttacgcag caggtcagac gcgtcacggc caatgccttc agcggtaact
    12601 tcgattgcct ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct
    12661 tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc
    12721 gcatatttga ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga
    12781 gtatcggtag atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca
    12841 gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag
    12901 acgctccagc ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat
    12961 gcctggtttt taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg
    13021 ttttcgatga gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc
    13081 aaatcgataa gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca
    13141 gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt
    13201 tggtatcttc agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca
    13261 tggattacaa caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac
    13321 agtgataggt tatgcctttt actcgacttt tgcactgact gaaaaggaca aattaatgtt
    13381 aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttcctgc
    13441 tccagtaaaa gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg
    13501 aggaatgaaa ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc
    13561 agatggtaag cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa
    13621 cacacttatc gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga
    13681 acaatcccac tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtgttcgc
    13741 cgatccgttc tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc
    13801 tggcaaagtg caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac
    13861 agcagcggca attcttgcct ccaaagatcc cgcaaaaacc tggcaagaat atgaatcctc
    13921 tggtggcaag cttaagctaa acgtgcctgc aaacgtaagt acagaacaaa tgaaagtgtt
    13981 aagcgccaac gagaaactca tggacgatct gggggcaaat gtcacaccag ctatctatta
    14041 catgagtaag gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa
    14101 tatcattatg gggaataaat aaaagataat tcatggatgg
//
LOCUS       DAJSKJ010000102        14117 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000102 DAJSKJ010000000
VERSION     DAJSKJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.102"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..205)
                     /locus_tag="ON345_002557"
     CDS             complement(<1..205)
                     /locus_tag="ON345_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3072604.1"
                     /translation="MSELSYLKKLLGGVEVEWKSLGEVAEIYGGLTGKTKADFEKGNA
                     RYISYKKIFGSLDIKNMPTDYVHV"
     gene            complement(202..1758)
                     /locus_tag="ON345_002558"
     CDS             complement(202..1758)
                     /locus_tag="ON345_002558"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3072605.1"
                     /translation="MTSLQQRAELHRQIWAIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYMEAGDESIHYAALDDSIITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNSRLAAVLKGVE
                     GLNLGNFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVMSKHKTDTRVQFIDASGLFKKETNNNILTDDHIEKIMQVFTSKADIDHL
                     AKTIPYETVAANDYNLSVSSYVEAMDTREIVDIAELNAELKITVSKIDQLRKDIDSIV
                     AEIEGSEAQA"
     gene            complement(1961..3826)
                     /locus_tag="ON345_002559"
     CDS             complement(1961..3826)
                     /locus_tag="ON345_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR3072606.1"
                     /translation="MAGQYEKAITIKQAIDSINLRHYLLPAIQRKFVWSSSQICLLFD
                     SIMRDYPINSFMMWDIRSASIKNDYKFYEFLKEYCQRFNEENPCVATNAGFHDFKAVI
                     DGQQRLTSLYIGLCGTYAYKQPRVWWPSAQDDRILPPRKLYVDLTAPLNSDDELMMKY
                     NFRFLTDKQYTDSLTDNKHHWFCLHEIFKYEQYDSPDDILFNVVVPELEKRGLISSEF
                     SRKTLLKLYTKIRTENLIHYFNESSQDIDHVLDVFIRTNSGGTKLEFSDLLMSIAVAH
                     WQGDFRKELDELTKNIYQNNEMGFYIERDWFLKTSLMLIDSDVRFKVKNFTSEEVGKI
                     QQQWSEIKSCIKETFILIRRFGINPQSLISKNAVIPVVYWLYKKQTSGHPLYTTINLL
                     NKNHNERSVISQWFYMVLLKGIFGSQADALLTSIRDVMKNSLSDIHFPLEKIIDRYKG
                     SNKDLRFDDEYIESLLNIRYGEGRCRALLHLLFPEMNPTEVFHIDHLHPRNHFSKKYL
                     EKLDYIANSPEKLSFYENPEYWDTIPNLHLLNHSQNISKQDTSLKQWLSQPSNNYSPS
                     MLLVSDDNIEFSRFPEFYNERRNALKQRLLSRVFLTTKIDSSPSTMDTDEEILTD"
     gene            complement(3900..4211)
                     /locus_tag="ON345_002560"
     CDS             complement(3900..4211)
                     /locus_tag="ON345_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072607.1"
                     /translation="MIIDKEYALVDATARLNTDLRDYEYEINNAAIITFGNDLIEVIV
                     YQFSFVISIRAEGEKIKHGLLVNFGKNIARQVSSLCASAMRVYPNEKHKPSRQLFHCI
                     N"
     gene            complement(4270..4983)
                     /locus_tag="ON345_002561"
     CDS             complement(4270..4983)
                     /locus_tag="ON345_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR3072608.1"
                     /translation="MTLITQENEHDRLATRLSIILSRLFQGEKLHIGTLAEEFGVTTR
                     TLRRDFNVRLTYLDIEQSNGVYQLASHYFRRRTERDMKILARLLHLDRLLPAMDAKLL
                     SLLLDGEHPSPYRIMLPEPRQKPTLFGDFSRLTLAILNQTQVRIRTDENVSHTVHPYR
                     LLCSHAEWFLAGCTSSGVFVISLSRIRLVEVLPDTTFEIDNTLISLIEQSDFMEALPH
                     MNIIHQIMHYGSKQTNNRN"
     gene            complement(5086..5835)
                     /locus_tag="ON345_002562"
     CDS             complement(5086..5835)
                     /locus_tag="ON345_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3944 domain-containing protein"
                     /protein_id="HCR3072609.1"
                     /translation="MATYRHDPDLEFLRHCHRDDLDLLVSVLIYDPKDGLARITETLT
                     YDPQYKKHKPNHPRYWDAIAAEVQTFGANSFATLLRWGKGVLYREILTDACDKMKVNY
                     NSKSSVETIEMNLLMKILEKSLEKMTPDQLKTVAEELQTGYSNPTPELLTMAIQAGIK
                     TSGFAAYQMALVVTNGVAKALLGRGLTVAGNAMLTRTMGAFAGPVGWVLSSLWLLIDL
                     AGPAYRVTLPSCVFIAYMRQKHLYSPENTDV"
     gene            complement(5978..6361)
                     /locus_tag="ON345_002563"
     CDS             complement(5978..6361)
                     /locus_tag="ON345_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072610.1"
                     /translation="MKILKLLTATILLSAFSHSAFADEQADAQMITNSTFCAMYSTRL
                     TQTSDSGLQVKGVNLNARFNGPVFNRVLQVMNQTYGRTWLESNARNGSMTAMQLSQSE
                     LLYNPEYARQCDAFADKVEKEWRGK"
     gene            complement(6439..6678)
                     /locus_tag="ON345_002564"
     CDS             complement(6439..6678)
                     /locus_tag="ON345_002564"
                     /inference="COORDINATES: protein motif:HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR3072611.1"
                     /translation="MARMATMNVNRRRQHATGQGERLIRLTMVLFYGQTISVRYIIRQ
                     FDVSPRTARRDLAQLAFVLESAGPHRWRLAPSLKP"
     gene            complement(6657..7121)
                     /locus_tag="ON345_002565"
     CDS             complement(6657..7121)
                     /locus_tag="ON345_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072612.1"
                     /translation="MYRLLLLNFVDSFIDNCILSCVDEPAYGGIVQCELLFGLAAHSG
                     IYVGNGDIVHLDGDGTVIKSKAKGFLDRLDGWNSAISVYTDCMDLTPQGCAWAAERAK
                     AAVGQRYPYSLLDFNCHRFTARCVTGNSCPDIWLARHLDSFQKGDRWRVWQR"
     gene            complement(7180..7608)
                     /locus_tag="ON345_002566"
     CDS             complement(7180..7608)
                     /locus_tag="ON345_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 family protein"
                     /protein_id="HCR3072613.1"
                     /translation="MSSPVILQGGYVLPLSRSFTQMLLDIVAQKPPHRHPITGLTINF
                     RDPEYSPETGGWHPVEIRLIPAGEDWQLDYVTDFHYAGHPWPELEKDVDVSWTQQYTW
                     LSRCGDISHVDAREFWSLWESNFMAYHDMGVFTVRTLWES"
     gene            complement(7655..8086)
                     /locus_tag="ON345_002567"
     CDS             complement(7655..8086)
                     /locus_tag="ON345_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072614.1"
                     /translation="MSKKKGATEAAVTESYTVFYSGEAEKISPHSKGLLTYELGKEGD
                     TGSLALRLTANGEGGLFSREWIALDAIHAILEQQQDSFPSRVFRPLFGQGSTNNAGFL
                     AAVLRSPDICLIEADSSRLFMHRCYADWQHRMTQLATLSQD"
     gene            complement(8079..8432)
                     /locus_tag="ON345_002568"
     CDS             complement(8079..8432)
                     /locus_tag="ON345_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072615.1"
                     /translation="MFKLTTPTSLLLLNLPASALAALSNEILGPVDDIDLFTTFWNET
                     GTLLWHLNHDDTLPEDPLLAVALANPEYVTALDDGWYLLLGIVCDNGQGIYLVFPDTT
                     VITQLQNLIEALTHE"
     gene            8876..10147
                     /locus_tag="ON345_002569"
     CDS             8876..10147
                     /locus_tag="ON345_002569"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3072616.1"
                     /translation="MQHIVKVVFTTLLLICSTLAYAQRLTVPAYMEDAVSCTTQPISQ
                     SLIQSVQAECIQKGKSRELSDSRTTGFKALVSKRGKVMFAARFSCGGSNVYRRLGDYP
                     ALTITQARSAAQKLIADMRAEYQLRGANLFVSHNLLFSGLLERFEHDVLDAGVKRSAD
                     TDRSKIRNYLLPLLGHFKIHDIDEGVIGRYLNGLSNLKPATRNRHLALLKAIFGYGRR
                     MKLVTTLPTEFIRMLPETTSRRRAMSHEEFQRWFKACEQKHHKEPDSAAVMLLMFLGL
                     TGLRLGETRHLRLEDVEWSRKVITLRQTKNGRQRHVPVCDKAFVLLTEARQFLGDSGW
                     VFPGQNTDNPVAEPRRLQKTLCETAGIPSYTIHELRHSFATILVESGADIHMVKELLG
                     HSTIKVTEIYVHSSPERYHAAVNQAMGVVNS"
     gene            10220..12145
                     /locus_tag="ON345_002570"
     CDS             10220..12145
                     /locus_tag="ON345_002570"
                     /inference="COORDINATES: protein motif:HMM:NF017826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCR3072617.1"
                     /translation="MSGDKLTNLADDIDTDIDLSLSQFLTQKSLNEARAAKILDDAIE
                     IELDEDEPSQNGSAAGLPAGYYNGNSGSFFARGKKVVHISSTPIIPCARCCSKNKQHN
                     MSVDLMTINYIGQKVVVSVSLDELARNPEQVIAALASRGFGVTTSVHARAYFEKFIHA
                     CMKMALPMYLVAESMGMVEGEAAFLHGDMPVARGISGFDYLVPHKSVFSGIRMSGSLA
                     EWKTLIEENVYGWPQLFALSSSFASMLLGMAGMDVALFHFYGGSTTGKTVVLQVGMSV
                     HAHGGEPGSHPDVAILRWNTTDNALEINLSEFSGLVACIDEMGAYSDRKFSSLLYNMT
                     SGRSKNRLDKSLTGRKPVLWKTFILSSGEMSIPEKLASRNEQLQGGQEHRAISLNILP
                     EDAAKEGEPVGEVRRRADILKAELAEQYGTAGKAFIACFLSQQNDDGSLMSYSELSEQ
                     IKETTEECCKMLAEDLQSDGYVLSDIQHRALKRFAICLVAGGMAAEWGILPFEPQMIN
                     DSVMSAVRRWLDDGRNQYNPVKEALSAIQLELIKRQAAHFVPLQDKEAYIPSNHWGYT
                     HPKTQDLMIFAPVFDDWCRRHNRKSAEVAKLLSAKNYLEPESKGHYKKRVQNSDVEGV
                     FYQIKRSFLHCNISAEF"
     gene            complement(12188..13375)
                     /locus_tag="ON345_002571"
     CDS             complement(12188..13375)
                     /locus_tag="ON345_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3072618.1"
                     /translation="MSSPKRFRFTSQLIKSLPPNSSDSRSTDAEYSDTEISGLKCLVS
                     KGEGRKKFLLRYLYHGRKRAIAIGHWPEVDVTLARKIAIEHKRSLATGTDPKQERENK
                     RQEMTFDELFNQHYLVWAKSAKRSWGKDVQRYRDHIRPAIGQMLLSDITPSAVLRLQQ
                     TLSMSLSAATCNRVIVLIKAVFTWAGRQSLTSVHPARVVQLLRENNARQRYFTEDEIR
                     RIFLSADKDISPVAARYVKLLLLTGLRRDELRLAKWEHLDPVKRTLWLPQTKNGYGRI
                     VHLNSLALDVIRTLPTQRGNPWLFIGKKQGMPLNNPVKAFQRIVRRAGIFDKEVCIHT
                     CRHSVAALIVSYGGTLYDVQSQLGHRSSQSSQRYAHLHPQRLQNTSQLLAERITAQLP
                     SLR"
BASE COUNT         3504 a         3209 c         3571 g         3833 t
ORIGIN      
        1 gaacatggac ataatcagtt ggcatatttt ttatatctaa gctaccaaaa atttttttgt
       61 atgaaatata cctggcatta cctttttcaa aatcagcctt ggtctttcct gttaatccac
      121 cataaatttc agcaacctca ccaagagatt tccactcaac ctcaacccca cccagcaatt
      181 ttttcagata actcagttcg ctcatgcctg tgcctcactg ccttcgattt cagccactat
      241 tgaatcaatg tctttgcgca actgatcgat tttgctgacg gtgattttta gctcggcatt
      301 cagctctgca atatcgacaa tttcgcgggt atccatggct tcgacgtagc tgcttactga
      361 caggttatag tcatttgcgg ctactgtttc ataagggatt gttttcgcta agtggtcaat
      421 atctgcttta ctggtaaaga cctgcataat cttttcgata tgatcatcag tcaggatatt
      481 gttattggtt tctttcttaa acaacccgct ggcgtcaata aactgaacgc gggtatctgt
      541 tttgtgcttt gacatcacca gaatgttaac ggcaatagtg gtgccgaaga acagattcgg
      601 tgccagagaa atcaccgttt caacatagtt gttgtcgacc aggtactgtc ggattttctg
      661 ctctgcaccg ccacggtaaa aaataccggg gaagcaaact attgctgcgc gccctttagc
      721 agacaggtaa ttcagcgcat gtagtacaaa cgcaaagtca gcttttgatt tcggggccag
      781 tacgccagca ggagcaaaac gctcatcgtt aatcagggtc gggtcatcac taccaatcca
      841 cttaaccgag tacggtgggt tagagacaat agcatcaaac ggtttttcat ctctgaagtg
      901 cggctcagtc agtgtattac ccagcttgat atcaaacttg tcgtagttga tgttgtgcaa
      961 aaacatgttc atacgcgcca ggttataggt cgtatggttg atttcctgac caaaaaaacc
     1021 ttcttcgatg atatggtcat caaactgttt tttagcctgc aacaacagcg aaccggaacc
     1081 cgctgccggg tcgtagattt tgttaacgtg ggtctgaccg tgcattgcca gttgtgcaat
     1141 cagcttagag acgtgctgcg gtgtgaagaa ctcccctcca gacttaccgg catttgccgc
     1201 atagttagaa atcaggaact cgtaggcatc accaaacagg tcaatctggt gctcgttaaa
     1261 attccccagg ttaagccctt caacgccttt cagaaccgcg gcaagacggc tgtttttgtc
     1321 tttaactgtg ttacccagtc gattgctggt cgtatcgaaa tcggcgaaca ggcctttgat
     1381 gtcggcctcc gacgggtagc cgtaggcaga gctttctata gcgacgaaga tactgtttaa
     1441 atcagcattt agcctgtcgt tggtgttcgc tttagcggca acgttgcaga acagctggct
     1501 cgggtagatg aaataacctt tagtcctgat ggcatcgtct ttgatgtcat cggtaatgat
     1561 gctgtcatca agtgctgcat aatgtatgct ctcatcccct gcctccatgt agctggaaaa
     1621 attctcactg atgaagcggt agaaaagtgc accgagtaca tactgtttaa aatcccatcc
     1681 atctacggag cctctgacat cattggcaat ggcccagatt tgacggtgta gctctgcacg
     1741 ctgctgaagg cttgtcatct tgtatcctgt tgtatgattc ttaagtactg ctattggccc
     1801 gattttatcg caatttcatt ccgttggcag gctttgtatg tcatgccttg cactgagagt
     1861 tcgtttgccc ggttatgtaa ccaacgctct ttcagtcagg ccagtagaaa cgttgtgctg
     1921 acaataaata ctgaggcccc aatactttcg gggcttaaga tcagtcagtg agaatttctt
     1981 catccgtatc cattgtggat ggtgatgaat ctatttttgt tgtaagaaac acccgactca
     2041 gtagtctttg cttcagggcg tttcttcgct cattgtagaa ttccgggaag cgactgaact
     2101 caatattgtc atctgaaact aacagcattg atggcgaata gttgtttgat ggctgagata
     2161 gccattgttt cagggaagta tcctgtttac ttatattctg agagtgattc agtaaatgaa
     2221 gattcggtat ggtgtcccag tattccggat tttcataaaa gctgagcttc tcaggtgaat
     2281 tcgcaatata atctagtttt tcaagatatt tctttgaaaa atgatttctt gggtgaaggt
     2341 gatcaatatg aaacacctcg gttggattca tttcagggaa cagaagatgc aatagtgcgc
     2401 ggcaacgacc ttcgccatat ctgatattga gaaggctttc gatgtattcg tcgtcaaatc
     2461 tgaggtcttt attcgagcct ttatatctgt caataatctt ttcaagagga aagtgaatat
     2521 ctgaaagact atttttcatt acatctctga tactcgtgag tagcgcatcg gcctggcttc
     2581 caaagatccc tttcagaagt accatgtaaa accactggct aattactgag cgttcattgt
     2641 ggtttttatt caggaggtta atcgttgtat ataatggatg tccactggtt tgctttttgt
     2701 aaagccagta gaccacagga atgactgcat ttttagatat caaagactgt ggattgatgc
     2761 cgaatcgcct gataagaata aaggtctcct tgatacagga ttttatttca gaccattgtt
     2821 gttgtatctt accgacctcc tctgaagtga agttttttac tttgaaccgg acgtcagagt
     2881 caataagcat caggctggtt tttaagaacc agtctctttc aatataaaaa cccatttcat
     2941 tattctgata aatgtttttt gtcagttcat ccagttcttt tctgaaatca ccctgccagt
     3001 gcgctaccgc tattgacatc agtaagtcgg agaactcaag ttttgtcccc ccgctgttcg
     3061 tgcggatgaa aacatccagc acatgatcaa tgtcctgact gctctcattg aagtagtgga
     3121 taagattctc agttcttatc ttggtataaa gtttaagcag agtttttctg gaaaattcac
     3181 tggaaatgag tcctcttttt tcgagttctg gtacgacaac gttaaataaa atatcatcag
     3241 gggaatcata ctgctcgtat ttgaatattt cgtgcaggca gaaccagtga tgtttgttgt
     3301 ctgtaagtga atcagtgtat tgcttgtcgg tcaggaacct gaagttatac ttcatcatga
     3361 gctcatcgtc tgaattaagt ggcgctgtta aatcgacgta aagctttctt ggcggcagga
     3421 tgcgatcatc ctgtgccgaa ggccaccaca cccggggctg tttatacgca tacgttccac
     3481 acaatccaat atataatgat gtgagacgct gctgaccatc aatcaccgcc ttgaaatcat
     3541 gaaatccagc atttgttgct acgcaggggt tttcttcatt aaatcgctga cagtattctt
     3601 tcaggaattc atagaatttg taatcgtttt taatactggc actacggata tcccacatca
     3661 tgaaagagtt tatcgggtag tctcgcatga tggaatcaaa cagcagacat atctgactgc
     3721 tgctccagac gaacttcctc tgaattgcag gcagaagata gtggcgtaaa ttaattgaat
     3781 ctatcgcttg ttttatcgtt attgcttttt catactggcc agccatcata aacctcatat
     3841 gttatattag ctggcctatt ttaatttatt gcgtgtcggt agtacagtct ttgcacaaac
     3901 taatttatgc aatggaaaag ctgacggctc ggtttgtgtt tttcattcgg gtagactcgc
     3961 attgcggatg cacaaagtga tgaaacctgt cttgctatat tttttccgaa gttaacaagt
     4021 aggccgtgct ttattttttc accctctgcc ctgatgctga tgacaaagct gaactgatat
     4081 acgattacct caatgaggtc attgccaaat gttatgatgg cggcgttgtt tatttcatat
     4141 tcataatccc tcaggtcagt gttaagtctt gcggtcgcat ctacaagtgc gtattcttta
     4201 tcgataatca taactttctc acttatcatg ttaatgtgat ttaatcatca ctaataatat
     4261 gttgtgattt taattacgat tattcgtctg tttactcccg taatgcatta tctgatgaat
     4321 aatgttcatg tggggtaagg cttccataaa gtcagactgt tcaatcagcg aaatgagtgt
     4381 gttatcgatc tcaaacgttg tatccggtaa tacctcaacc agcctgattc gggacaggga
     4441 aataacgaac acgcccgatg acgtacatcc ggcaagaaac cactccgcat ggctgcaaag
     4501 cagacggtag ggatgaacgg tatggctgac gttttcgtcg gttctgatac gcacctgggt
     4561 ctggttaagt atggcgagcg tcagtctgct gaaatcacca aagagcgtgg gtttctggcg
     4621 cggctccggg agcataatcc ggtagggtga agggtgctcc ccgtcaagca gaaggctcag
     4681 cagtttggcg tccatcgcgg gtaacagacg gtcaagatgc agcaacctcg ccagtatctt
     4741 catatcccgt tccgtgcggc gacgaaagta atgtgaggca agctgataca cgccgttgct
     4801 ctgttcaata tcaaggtagg tcagacggac gttgaaatcc cgtcgcagcg ttcgcgtcgt
     4861 cactccaaat tcttctgcca gcgtcccgat atgcagcttt tcaccctgaa acaaacggct
     4921 caggatgatg gaaaggcggg ttgccagtct gtcatgttcg ttttcttgtg ttatcagcgt
     4981 catacatcat ttccgaattt agccgggaaa taatgccagc agtagcggac aggatttgtc
     5041 cgccctctga agttttttaa agtctgaata ccttaaatgt aagtgctaaa cgtcagtatt
     5101 ctcaggtgaa taaagatgct tctggcgcat gtaggcaata aaaacgcagg atggtagcgt
     5161 cacccggtat gcgggaccgg ctaaatctat cagtagccag agagaactga gcacccagcc
     5221 cacgggaccg gcaaaagcgc ccatagtccg tgtcagcatg gcatttcctg caacggtcag
     5281 ccctctgcct aacagggctt ttgctacgcc atttgtgaca acaagcgcca tctgataggc
     5341 cgcaaatccg gatgttttta tccccgcctg gattgccatg gtcagcagct ccggtgtggg
     5401 attgctgtag cctgtctgca attcttccgc cacagtcttt agctggtcgg gcgtcatctt
     5461 ctcaagactt ttctccagaa tcttcatcag cagattcatt tcaatggttt caacactgga
     5521 tttgctgttg tagttcacct tcattttatc gcaggcatcg gtaagaattt cccggtacag
     5581 caccccttta ccccagcgga gcagcgtggc aaagctgttc gccccaaacg tctgaacctc
     5641 ggcggcaatg gcatcccagt agcgcgggtg gttgggcttg tgttttttat actgaggatc
     5701 ataggtcagc gtttccgtga tgcgtgccag tccgtctttc ggatcgtaga taagcactga
     5761 aaccagtaaa tccagatcgt cacgatgaca gtggcgcaga aactcaaggt cggggtcgtg
     5821 tcggtaggta gccatatttc acctcattgc agatattgga cctgacagtt taaggaagcc
     5881 cccggacagg gtttgtccgc ctgaaaaaca aaaaagtaaa aaaaacctgc ccgaaagcag
     5941 gtttctgttc accggatgga ggtataacgc gtcgggatta ctttccgcgc cattcctttt
     6001 ccactttatc cgcaaaggca tcgcactgcc gggcatattc cgggttatag agcagctccg
     6061 actgcgaaag ctgcatggcg gtcatgctgc cgttacgggc gttcgattcg agccaggttc
     6121 tgccataggt ttggttcatc acctgaagta ccctgttgaa taccggtccg ttgaagcggg
     6181 cattgagatt aacgcctttt acctgaaggc cgctgtcact ggtctgggtc aggcgggtgg
     6241 agtacatcgc gcagaaggtc gagttggtta tcatctgagc gtcagcctgc tcgtcggcga
     6301 acgcgctgtg gctgaaggcg gaaagcagaa ttgtggcggt taacagtttt aaaattttca
     6361 ttatgttccc ttatggattg aaaagcggag acagcctgcc atacggggtg gtcaggagat
     6421 gtccggcaga gaaaaaagtc agggtttcag cgagggagcc agtcgccagc ggtgagggcc
     6481 agcggattcg agcacaaagg ccagttgagc gaggtcccgt cttgcggtcc ggggagatac
     6541 gtcaaactgc cggatgatgt agcgaacaga tattgtctga ccgtaaaaca gcaccatggt
     6601 cagccggatg agccgttctc cctgtccggt agcgtgttgc ctgcgacgat taacgttcat
     6661 cgttgccata cgcgccatcg gtcccctttc tgaaaagaat cgaggtgtct ggcaagccag
     6721 atatcagggc atgaattgcc ggtaacgcag cgggcagtaa accggtggca gttaaagtcg
     6781 agcaggctgt agggatagcg ctgacccact gccgcctttg cccgttcagc ggcccacgca
     6841 caaccctgag gcgtcaaatc catacagtcg gtatacaccg agatagcgga gttccagcca
     6901 tcgagccggt caagaaatcc tttagcctta cttttgataa cggttccatc gccatcaaga
     6961 tgaacaatat cgccgttacc cacatagata ccgctgtgtg cggcaaggcc aaaaagcagt
     7021 tcgcactgca cgataccacc gtaagccggt tcatcgacgc aggagagaat gcagttatcg
     7081 ataaatgaat ccacgaagtt cagcagcaga agccggtaca taacattcag ttccttacta
     7141 cgggggagag gggggatgcg ggggaaacgt agtgaacact cagctttccc acaacgtacg
     7201 gaccgtgaaa acccccatat cgtggtacgc cataaagtta ctttcccata gcgaccagaa
     7261 ctcacgggcg tcgacatgag agatatcgcc acaacgggaa agccacgtgt attgttgcgt
     7321 ccagctcaca tcgacatctt tttcaagctc aggccatgga tgtccagcat agtgaaaatc
     7381 ggtgacgtaa tcgagctgcc agtcttctcc ggcagggatg aggcgtattt cgaccggatg
     7441 ccagccacct gtttccggtg agtattcagg gtcacgaaag ttaatggtta atccggtgat
     7501 agggtgacga tggggcggtt tttgcgcgac gatatccaga agcatttggg tgaacgaccg
     7561 ggagagcggc agcacatagc cgccctgcaa tattacgggt gatgacatag tgaatttcct
     7621 gatttttggt taacagaaca cagaaaaagc ggggttagtc ctggctgagc gtagcaagct
     7681 gcgtcatacg atgctgccag tcggcatagc agcggtgcat aaagagtctg ctgctgtccg
     7741 cttcgataag gcagatatcc ggacttctga gcacagcagc aagaaacccg gcgttgttgg
     7801 tactgccctg accaaacagt gggcgaaata cacgggacgg gaagctgtcc tgctgctgtt
     7861 ccagaatggc gtgaatcgca tcaagcgcta tccattcacg agagaacagg cctccttcgc
     7921 cattggcggt taagcgcagg gcaaggctgc cggtatcacc ctccttaccc agctcatacg
     7981 tcagcagacc tttactgtgg gggctgattt tctcggcttc accactgtag aacacggtat
     8041 agctttcggt gacggcggct tcggtagcgc cttttttctt actcatgggt cagcgcctca
     8101 atcaggttct ggagttgagt gataacggtc gtgtccggga acaccagata gataccctga
     8161 ccgttatcgc agacgatgcc gagcaggaga taccagccat catcaagcgc ggtgacgtat
     8221 tcagggttag ccagtgccac tgccagcagc gggtcttcgg ggagtgtgtc atcgtggttg
     8281 agatgccaga gcagggttcc cgtttcgttc cagaaggtgg taaacaggtc gatatcatcg
     8341 acagggccaa ggatttcatt gctgagggcc gccagtgcgg aggcgggcag gttgagaagg
     8401 agaagactgg tgggggtcgt gagtttaaac atgattatct ccatttgatt gatttcaaat
     8461 ggataatatg tgcctgtttt gtggcgtgtt acggcgaagt atggaaaaag gttttgacat
     8521 cgtggatttt ttccagttac atttatcccg ccttcagtgc aggtctgttc atggccagta
     8581 agcattaagg agtccagcgc ggctaccccg atgaaccatc aggtgagtcg ttgactggta
     8641 cagcggtcaa tgatgtatgc aggttatatc ctgccatctt tcgctgttct gagccttcac
     8701 agtgatgtgt tgtttcgggg cgctttatca gtgccctgct cgagcctgta agaaattacg
     8761 ggaccacaag cgtcttatgg cgccaatggt atctgctggt gaatccagtg gattaacaca
     8821 cgaaattcaa cgggatctct gatccttttg tttaattttc gcgtggattc gggctatgca
     8881 acacattgtc aaagtagtat ttacaaccct tctcttgatt tgcagcaccc ttgcgtatgc
     8941 acagcgttta accgttcccg catacatgga ggacgctgtg tcctgtacaa cacaacctat
     9001 aagccagtcc cttattcaga gcgttcaggc tgaatgcatt cagaagggca aaagtcgtga
     9061 actgagcgac agcagaacta cgggttttaa ggcgctggtc agtaaacgag gcaaggtcat
     9121 gtttgccgcc cgtttcagtt gtggcgggag taacgtatat cgccgtctgg gtgattatcc
     9181 ggcacttacc atcacgcagg cgcggtcagc agcacaaaaa ctaattgcag atatgcgggc
     9241 agagtaccag ctacgtggtg ctaacctgtt cgtttcgcat aatttgctgt tcagtggtct
     9301 gctggagcgc ttcgaacatg atgttctgga cgctggtgta aagcgttctg ccgataccga
     9361 tcgttcaaaa atccgtaact atctgcttcc ccttcttggg cattttaaga ttcatgatat
     9421 cgatgagggc gttatcgggc gttatctcaa tggcctgagc aatctgaaac cagccacacg
     9481 taatcgtcat ctggctctgc tgaaagccat ttttggttat ggtcgcagaa tgaagctggt
     9541 gacgacgtta cccactgagt ttatccggat gctgccggaa accacgtccc gtcgcagggc
     9601 gatgagccat gaggaatttc agcgctggtt taaggcctgc gagcaaaaac accacaagga
     9661 acccgatagt gcagcggtca tgctgctgat gtttctgggc ttaaccgggc ttcgactggg
     9721 ggaaactcgt caccttcgtc tggaggatgt ggaatggtca cgtaaggtca ttacgctcag
     9781 acagaccaag aacggcaggc agcgtcatgt tccggtgtgt gataaagcct ttgtgctgct
     9841 gaccgaagcg cgtcagttcc tgggggatag cggttgggtt ttccctggac aaaacacgga
     9901 taatccggtt gcagaacccc gtcgcctaca aaaaacactt tgtgaaacag cgggtatccc
     9961 gtcctatacc atccatgagt tgcgccattc ttttgcgaca atccttgtcg aaagtggcgc
    10021 ggatatccac atggtcaagg agctgctggg ccacagtacg attaaagtca ctgagatcta
    10081 tgtgcattcc agtccggagc gatatcacgc tgccgtcaat caggcaatgg gcgttgttaa
    10141 ttcgtaaaca gaaccatcgc agcaggcgga tgggttctgg cttacgcctg ctgttcatat
    10201 tgaatttaag gagacagtca tgagtggcga taaattaacc aatctggcag atgatattga
    10261 tactgatatt gatctgtcac ttagtcagtt ccttacgcaa aagtcgctta atgaggcgcg
    10321 tgcggcaaaa attcttgacg atgcaataga gattgaactg gatgaagatg aaccgtcgca
    10381 gaacggaagt gcagcgggac ttcctgcggg ctactataac gggaactcgg gtagcttttt
    10441 cgccagaggg aaaaaggttg tacatatcag ttccactccc ataatcccat gcgccagatg
    10501 ctgctctaaa aacaaacagc acaacatgag cgttgatctg atgaccatta attacatcgg
    10561 gcaaaaggtc gtggtgtcgg tatcgcttga cgagctggca agaaacccgg aacaggtcat
    10621 tgctgctctg gcgtccagag gatttggtgt gacgacatct gtccatgcca gggcttactt
    10681 tgagaaattc attcatgcgt gcatgaaaat ggcgctgcca atgtatctgg tggcagagtc
    10741 tatgggtatg gtcgaaggag aagctgcctt tttgcatggt gatatgccgg ttgccagggg
    10801 gatctccggt tttgattacc ttgttcctca taaatcggtt ttttcaggca ttcgcatgtc
    10861 agggtcgtta gcagaatgga aaacgcttat tgaagagaat gtttacggat ggccccagtt
    10921 gtttgcgctc agcagctcgt ttgcatcaat gctacttggg atggccggga tggatgtggc
    10981 gttgtttcat ttttatggtg gttcgacaac agggaaaacg gtagttcttc aggtgggaat
    11041 gtctgtacat gcccatggcg gtgagcccgg tagtcatccg gacgtcgcta ttctgcgatg
    11101 gaatacaaca gataatgcac tcgaaatcaa cctcagcgag ttttccgggt tagttgcctg
    11161 tattgatgaa atgggcgctt acagtgatcg aaaattctca tcattgttgt ataacatgac
    11221 ctctggtcgc tcaaaaaatc gcctggataa gtcgctcaca ggaagaaaac ccgtattgtg
    11281 gaagacgttc attctgtcct ccggcgaaat gtctataccg gaaaagctag cttcccgcaa
    11341 tgaacagctt cagggagggc aggaacatcg ggctatcagc ctgaatattc tgccggaaga
    11401 cgctgcaaaa gaaggtgaac ccgtcggaga ggtacgtcgc cgcgctgata tcctcaaagc
    11461 tgaactggcg gaacagtatg gcacggctgg aaaagccttt attgcctgct ttttgtcgca
    11521 gcagaatgat gatggtagct tgatgtcata cagtgaatta tccgaacaga taaaggagac
    11581 gacagaagaa tgttgcaaga tgctcgcgga ggatctgcag tcagacggat atgttctttc
    11641 cgatattcag caccgggcgt taaaacgctt tgctatttgc cttgttgctg gcggtatggc
    11701 tgcggagtgg ggcatcctgc catttgaacc gcaaatgatt aacgattcgg tgatgagcgc
    11761 ggttcgccgc tggctggatg atggacggaa ccagtacaac ccggttaaag aagcattaag
    11821 cgccattcaa ctggaactta tcaaacgaca ggccgcgcat tttgtaccgc tacaggataa
    11881 agaggcttat atacccagca atcactgggg gtatacacat cctaaaactc aggatttgat
    11941 gatttttgcg cctgtatttg acgactggtg caggcgacat aaccgtaaat ccgcagaggt
    12001 agctaagctc ctttctgcca aaaattatct tgagccggaa agtaaagggc attacaaaaa
    12061 acgtgttcag aacagtgatg ttgaaggcgt tttctaccag ataaaacgat cgtttctcca
    12121 ctgtaatatt tcagccgagt tctgatggta tgcaaaaaaa gcaggggcat ttgcctctgc
    12181 ttttctgtta tcgcaaggac ggtagctgag ctgttatcct ttccgccagt agctggctgg
    12241 tgttctgtaa gcgctgagga tgaagatgcg cataccgttg tgatgactga ctgctgcgat
    12301 gccccagctg agactgaacg tcatacagtg ttccaccgta ggaaacgatc agtgcggcaa
    12361 ccgaatggcg acaggtatga atacacacct ctttgtcaaa aatgcccgcc cgacgaacga
    12421 tccgttgaaa ggctttaacc ggattattca gcggcattcc ctgctttttg ccgataaaca
    12481 accatggatt tccccgctgc gttggcagtg ttctgataac gtccagtgcc agtgagttaa
    12541 gatgaacaat ccgcccatag ccattcttgg tttggggtag ccataaggtg cgttttaccg
    12601 ggtcaaggtg ttcccacttt gccagacgta gttcatcgcg ccgaagcccg gtgagcagca
    12661 acagtttgac atagcgggca gcgacggggc tgatatcttt atcggcggag aggaaaatac
    12721 gacgaatttc gtcctcggtg aaatagcgtt gccgggcgtt gttttccctg agaagctgta
    12781 ccacccttgc cggatgaaca gaggtaaggc tttgtctccc ggcccaggta aacaccgctt
    12841 taatgagtac gatgacacgg ttacaggtgg ctgctgacag tgacatactt agcgtttgct
    12901 gaagccgaag aacggcgctg ggtgtgatat cgctcagcag catctgacca atggcggggc
    12961 ggatatgatc ccggtagcgc tgaacatcct tgccccaact gcgtttcgcg gattttgccc
    13021 aaaccagata atgctggtta aagagttcat caaatgtcat ctcttgtctc ttgttttcac
    13081 gttcctgctt agggtcagtc ccggtggcaa gcgagcgttt atgctctatg gcaatcttgc
    13141 gtgctaaggt gacatcgacc tcaggccagt ggccaatggc gatggcgcgt tttcgtccat
    13201 gatagaggta gcggagcaag aattttttac gtccttcacc tttgctgacc agacacttca
    13261 gacccgaaat ctcggtatcg ctgtattcgg catcggtact gcgtgaatct gatgagtttg
    13321 ggggaaggga tttaatgagc tgactggtaa aacggaaacg tttcggactg gacatattag
    13381 ctcctgtgac tggtgtaaag aacgtcaggg ttgacccatc cccggcacgg cgctgttaat
    13441 ggctgagttg attttctggt gtggcgaagg gaatgctggt gccacatggg atgaatgaag
    13501 atgggagatg cagagagagc aggctttacg gtctgcttct ggtcttaagg agaaagattg
    13561 ccgttgggga aaagttcaca gaaataatat gtgtttcagt cggatataat tacatgtgat
    13621 aagggcaaag agcgtgcttt gctaaaattt gacatgttga agtccactct ttttaacgat
    13681 gtcgttatgg acgacatggc gagtcttgct tagataatta atcaaaatac tgtgactttg
    13741 gacccacgtt taccacattt cgagcacttg agccgagttt taaggtcttc tagagttact
    13801 cccattgcaa catactgctt aaggtcgttc aaagtaagga ttgtggggag acgacaaaaa
    13861 aatccagagg cgcaacatac cagcatctcc gagccgtttt ttaaccagtc ctcaagatta
    13921 tcgtgcgatc tcgttagtcg ttcgatatcc gctctccact gctggatttc attaccgttc
    13981 cagtcgatat ttagctcgat ttgtttggca acgaaccggt gtttacctct gttaactgtg
    14041 ggttcatagt ccttacgttg atctttgatg agatcagcct catagaggaa atcatcgttt
    14101 ttgttgtcgg tataatc
//
LOCUS       DAJSKJ010000103        14029 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000103 DAJSKJ010000000
VERSION     DAJSKJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.103"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            21..715
                     /locus_tag="ON345_002572"
                     /pseudo
     CDS             21..715
                     /locus_tag="ON345_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019082309.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
     gene            711..1280
                     /locus_tag="ON345_002573"
                     /pseudo
     CDS             711..1280
                     /locus_tag="ON345_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1505..2656)
                     /locus_tag="ON345_002574"
     CDS             complement(1505..2656)
                     /locus_tag="ON345_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR3072619.1"
                     /translation="MAYLDPYQRPAPDRFVRRGLFITACIAALMLLWQFLPAIEAWFS
                     PREAAERTVMARGDLAADEKTTIELFEKSRASVVYITTAQLVRDVWTRNVFSVPRGTG
                     SGFIWDDAGHVVTNFHVIQGASEATVKLADGRDYQAALVGTSPAHDIAVLKIGVGFKR
                     PPAVPVGTSADLKVGQKVFAIGNPFGLDWTLTTGIVSALDRSLPGEAGGPAIDHLIQT
                     DAAINPGNSGGPLLDSAGRLIGINTAIYSPSGASAGIGFAVPVDTVMRVVPQLIKTGK
                     YIRPALGIEVDEQINARLQALTGSKGVFVLRVTPGSAAHRAGLVGVEVTAGGIVPGDR
                     VISIDGIAVDDVTTLQARLDDKNVGDVVVLLVERAGKTREMLVELQPGV"
     gene            complement(2681..2944)
                     /locus_tag="ON345_002575"
                     /pseudo
     CDS             complement(2681..2944)
                     /locus_tag="ON345_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent protease"
     gene            3002..4024
                     /locus_tag="ON345_002576"
                     /pseudo
     CDS             3002..4024
                     /locus_tag="ON345_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
     gene            4026..4970
                     /gene="mhpB"
                     /locus_tag="ON345_002577"
     CDS             4026..4970
                     /gene="mhpB"
                     /locus_tag="ON345_002577"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCR3072620.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQAVLDEVNGVIASARARIAAFEPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPTDLAEACAHAVMKSG
                     IDLAVSYNMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FASSLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKQLPENERELRQQRVIS
                     AAEKFVVDQNTLHPLNPVWDNRFMSLLEQGRLQGLDAVSNEELSAMAGKSTHEVKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSATTQN"
     gene            4999..5865
                     /locus_tag="ON345_002578"
     CDS             4999..5865
                     /locus_tag="ON345_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3072621.1"
                     /translation="MTYQPQTEAATSRFLEVQEAGETLRVHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVQAGYRVILLDCPGWGKSDGIVNRGSRSDLNARILKSVVDQ
                     LDIQTVHLLGNSMGGHSAVAFTLSWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYREPTIENLKLMMEIFVFDTSDLTEALFKARLNNMLSRRDHLENFVQSLEANPKQFP
                     DFSPRLGEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIYRDCGHWAQWEHADA
                     FNQLVLNFLTRP"
     gene            5875..6684
                     /gene="mhpD"
                     /locus_tag="ON345_002579"
     CDS             5875..6684
                     /gene="mhpD"
                     /locus_tag="ON345_002579"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCR3072622.1"
                     /translation="MTNRNLEQLAADLRQAEEKGEAIAPLRELIGVDNAEAAYAIQRI
                     NVQYDVARGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPNTDTTFDELYNAIEWVLPALEVVGCRIRDWSIKFVDTVADNAS
                     CGVYVVGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPINAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIDGIGSVAATFSAAVTKGSQS"
     gene            6681..7631
                     /locus_tag="ON345_002580"
     CDS             6681..7631
                     /locus_tag="ON345_002580"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HCR3072623.1"
                     /translation="MSKRKAAIIGSGNIGTDLMIKILRHGVHLEMAVMVGIDPESDGL
                     ARARRMGVATTHEGVTGLIAMPEFADIDIVFDATSAGAHVKNDAALRAVKPGIRLIDL
                     TPAAIGPYCVPVVNLAANVDQQNVNMVTCGGQATIPMVAAVSRVAKVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSKAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSEAAS
                     EVEVEASINEMAVAVQAYVPGYRLKQRVQFEVIPEDKPVNLPGIGQFSGLKTAVYLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARQAGEAA"
     gene            7628..8641
                     /gene="dmpG"
                     /locus_tag="ON345_002581"
     CDS             7628..8641
                     /gene="dmpG"
                     /locus_tag="ON345_002581"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCR3072624.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAYQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAQQAKLMENYGA
                     TCIYVVDSGGAMNMSDIRARFRALKAVLKPETETGMHAHHNLSLGVANSIAAVEEGCD
                     RVDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCEVAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NRK"
     gene            8711..9922
                     /gene="mhpT"
                     /locus_tag="ON345_002582"
     CDS             8711..9922
                     /gene="mhpT"
                     /locus_tag="ON345_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCR3072625.1"
                     /translation="MTTRTSASYRLTLTVGLCFLVALMEGLDLQAAGIAAVGIAHAFA
                     LDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIASVALFGLFSLATAISWDFS
                     SLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAAALGFSG
                     LASAWQTVFWVGGVIPLLLVPLLMRWLPESQVYTQQTQVAAAPLRALFAPATASATLL
                     LWLCYFFTLLVVYMLINWLPMLLVEQGFRPSQAAGVMFALQIGAASGTLILGALMDKL
                     RPVVMSLLIYTGMLASLLALGTVSSLSGMLMAGFVAGMFATGGQSVLYALAPLFYSTQ
                     IRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILLAGLAVFILMSR
                     KSQMPIPASGA"
     gene            10197..11600
                     /gene="mmuP"
                     /locus_tag="ON345_002583"
     CDS             10197..11600
                     /gene="mmuP"
                     /locus_tag="ON345_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HCR3072626.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            11587..12519
                     /gene="mmuM"
                     /locus_tag="ON345_002584"
     CDS             11587..12519
                     /gene="mmuM"
                     /locus_tag="ON345_002584"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HCR3072627.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(12628..13674)
                     /gene="fbpC"
                     /locus_tag="ON345_002585"
     CDS             complement(12628..13674)
                     /gene="fbpC"
                     /locus_tag="ON345_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HCR3072628.1"
                     /translation="MTQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVPRAELKARVKEALAMVDLEGFEDRFVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVR
                     PEAITLSDRGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYL
                     EIHPYGMFVLADAA"
     gene            complement(13686..>14029)
                     /locus_tag="ON345_002586"
     CDS             complement(13686..>14029)
                     /locus_tag="ON345_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3072629.1"
                     /translation="IDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAI
                     TTVSAIVFLVTPDIRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFIFDWLIGES
                     RTSRSKAKNQA"
BASE COUNT         2760 a         3877 c         4380 g         3012 t
ORIGIN      
        1 tttccctata gtgatgattc atgcttggtt ccctttctaa gcggcttgag gacttgagtt
       61 tggtccagcg cgagaggttg gcctacatcg attttcggct ctatttcttc ggtgagatcg
      121 gacgccctga tctgattgag cgcttcggtg tggctccggc aggggcgaca cgcgatttgg
      181 ctttgtaccg ggaaattgcg ccgcataaca tcaccttcga tggtagcaac aagatttatc
      241 gcatcgggca ggagttctca ccgttgttcg agcacgcatc gctgcgcgtt ctgtcggcgc
      301 tgtctctcgg ttttggcgat ggcgtgaacg gctcaatgca ggcgctgcta ccatgcgaat
      361 cccccgcagc cctaagcatt cccaagatgg atgtgttagc cacgatttgc cgaggcatcc
      421 acgctaagcg acccgtcgcc atccgctact actcgatgag cagcggcgag tccgagcggg
      481 taattgtgcc ctttgctttg gttgataccg gcatgcgctg gcacgtccct ttgatcgcaa
      541 gagtagagag tttcgggatt tcgtcgtcac ccgcatcgaa gcaccaacat tgctcgacga
      601 ggagccacaa gccaacgaac gacaggataa cgacatccag tggacgcgca tcgtcgagct
      661 ggatttcgtg ccgtacccga gcgttgagcg gcccgatatt atcaagatgg actatcgtcg
      721 atgccacgct gatccacgcg cccagttcat ccaagaacca ggacggcaag cgcgacccga
      781 aaatgcacca gaccaagaag gggaaccagt attactttgg catgaaggcg cacatcggcg
      841 ttgatgatga atcggggctg gtacacagcg tggtgggcac ggcagccacc gtggcggatg
      901 tcacccaggt cgacaagctg ctgcacggcg acgagaacgt cgtctgcgcc gatgccggct
      961 acaccggcgt cgaaaagcgc tccgggcatg ccgaccgaga agtgatctgg caggtcgcag
     1021 cacgccgcag cacctacaag atgctcgata aacgcagcgc cctgtacaaa gccaagcgca
     1081 agatcgagaa ggccaaggcg caagtgcgag cgaaggtaga gcccccgttt cgcgtgatca
     1141 agcggcagtt cggctacacc aaggtacgtt tccgagggct ggtgaggaac actgcacaac
     1201 tggtgacgct gtttgccctg tcgaacctat ggatggcccg ccgacatttg ctggcaagcg
     1261 caggagaggt gcgcctgtaa tgcggaaaat ggccgtcgca aggtgctcgc gacggctgaa
     1321 agcaccgaaa tgagcgggtg atccgatcgt ttttgatcgg ttcaccgctt tcaaaatcag
     1381 cagtggctga agtcagccgg aaatacaggg ctacttcaga ccatccctag ctagggctcg
     1441 tagttcggcg caaggctgaa gtcgcttggc tgcttcgctg tgcagatcca tgacccactt
     1501 tccatcaaac tcccggttgt agttccacaa gcatctcgcg agtcttgccg gcccgctcca
     1561 ctaacaagac cacaacatct ccaacgttct tgtcgtctag ccgggcctgt aatgtggtga
     1621 cgtcgtcgac ggcgataccg tcgatgctga taacgcgatc gccgggcacg atgccgcctg
     1681 cggtgacctc gacgccgacg agcccggccc tgtgcgccgc cgagcccggc gtcacgcgca
     1741 atacgaatac gcccttactg ccggtcagcg cctgcagacg cgcgttgatc tgctcatcca
     1801 cctcgatgcc cagtgccgga cggatgtact tgccggtctt tatgagttgc ggcaccacgc
     1861 gcatgacggt atcgaccggc accgcaaagc ctatgccggc cgaggcgcca gacggactgt
     1921 agatggcggt attgattccg atcagccgcc cagccgaatc gagcagcggg ccaccggaat
     1981 tgccggggtt gatggcggcg tcggtctgga tcaggtgatc gatggccggg ccgcccgctt
     2041 ctcccggcaa cgagcggtcg agcgcggaga cgataccagt ggtgagcgtc cagtccaggc
     2101 cgaaggggtt gccgatagcg aacaccttct gacccacctt gaggtcggca ctggtgccga
     2161 ccggcacggc cggcgggcgc ttgaaaccga cgccgatctt gagcacggcg atgtcgtgcg
     2221 cggggctcgt ccccaccagc gcggcctggt agtcgcggcc gtcggccagt ttgacggtgg
     2281 cttcggacgc gccctggatg acgtggaagt tggtgacgac gtggccggcg tcgtcccaga
     2341 tgaagcccga gccagtgccg cgcggcacgg agaacacgtt gcgcgtccag acatcgcgta
     2401 ctaattgcgc ggtggtgatg tagaccaccg aggcgcgcga tttttcgaac agttcgatgg
     2461 tggttttctc gtccgccgcc agatcgccac gcgccatcac ggtgcgttct gctgcctcgc
     2521 gcggactgaa ccatgcctcg atggcgggca gaaactgcca cagcagcatg agcgcggcaa
     2581 tgcaggcggt gatgaagagc ccgcgccgga cgaagcggtc cggcgcgggg cgttggtacg
     2641 ggtcgagata ggccatgaga cgtctcctgt tgatgggcaa tcagcgccac agcccgctcg
     2701 ggcgccagcg cggtgggcgc gtcgccaaag cggattccgg caaaaaatgg ggcgcgcgga
     2761 gtggcaacgc tgatgctggc ccaggcgcca acgtcagcag gcgtgcgatg cgctcttgcg
     2821 ttgccggacg cgtacgcaag caggagtgct cgggattgcc ccatcccggc catagccagg
     2881 cacgccagga gcggctgacc cgctcgattt ttgccagcac ggacgccagc ccctgcgggt
     2941 cgccagctcg cgtgggctca ggctgcgcag caggccatcg gtgagggcga tcgatgcctc
     3001 ctgctgcgat ccggtgcgcc cgtatgtctc tgccgcgctg ccgcacgcgg tgcgccgttt
     3061 tgagtttatg gttatgccgg gggaaaccga agcgcagctt agcgagccgc agaatatgcg
     3121 ccagttactg agcaaagtgc tgccgaaccc ggacaacgtc gagctgatcc gccagcgcgt
     3181 gtatacccat aatgctcgtc tggccgaacg ttttcgtatt gaccgcgtgc tgctggcggg
     3241 cgatgcggcg cacattatgc cggtctggca gggtcaggga tacaacagcg gcatgcgcga
     3301 tgctttcaac ctggcatgga aactggcgct ggtgattaac ggtaaagccg acgatgcgct
     3361 gttggatacc taccagcagg aacgccgcga ccacgccaaa gcgatgatcg acctctccgt
     3421 gaccgctggc aacgtacttg cgccgcccaa acgctggcac ggcgcggtac gcgatggggt
     3481 ttcctggctg ttgaactata ttccgccggt caaacgctac ttccttgaaa tgcggtttaa
     3541 gccgatgccg cagtaccacg ccggggcgct ggtgcgtgaa ggcgacgcga aaacttcacc
     3601 ggtcggcaag atgtttatcc agccgaaggt gacgctggaa tcgggcgaag tgacgttgct
     3661 cgacaacgtc atcggaccga attttgcggt gattggctgg ggctgtaacc cactatgggg
     3721 catgagcgag gcgcagatcc aacagtggca ggcgctgggg acgcgtttta tccaggtcgt
     3781 gccggatacg cagatacata ccgatcagga taaccacgac ggcgttaccc gcataggcga
     3841 tacgcaaaac cgcctgcgcg cgtggtttgc ccagcacaac gccgcgctgg tagtcatgcg
     3901 cccggaccgc ttcgtggccg ccatcgccat tccgcaaacc ctgggcagca ccctgaataa
     3961 actggcgtcg gtgatgacgc tcactcgcgc tactgccgac attcctgttg aaaaggttgc
     4021 ctgatatgca cgcttatctt cactgtcttt cccactcgcc gctggtgggc tatgtcgacc
     4081 cggcacaggc tgtgctcgac gaggttaatg gcgtgatcgc cagcgctcgg gcacgtattg
     4141 cggcgttcga acctgaactg gtcgtgctgt ttgcccccga tcactacaac ggatttttct
     4201 acgacgtgat gccgccgttc tgtttaggcg ttggcgctac ggcgatcggc gattttggta
     4261 gcgcggcggg ggaattaccg gtgccgacag atctggctga agcctgcgcc cacgcggtaa
     4321 tgaaaagcgg tatcgatctc gccgtgtcct acaacatgca ggtcgatcat ggcttcgcgc
     4381 agccgctgga gtttttgctc ggcggcctgg ataaggtgcc ggtgctgcca gtgtttatta
     4441 acggcgtcgc aacgccgctg ccgggctttc agcgtacccg catgctcggt gaagcgattg
     4501 gccgtttcgc cagctcgctg aataagcgcg tgctgttttt aggctccggc gggctatcgc
     4561 atcagccgcc ggtaccggag ctggcgaagg ctgatgccca tatgcgtgac cgcctgctcg
     4621 gcagtggcaa acagttacct gaaaacgagc gcgagctgcg ccagcagcgg gtgatcagcg
     4681 ccgccgagaa gtttgtggtc gatcaaaaca cgctacaccc gcttaaccct gtctgggata
     4741 accgttttat gtccctgctt gagcaggggc gtttgcaggg gctggacgcg gtcagtaacg
     4801 aagagctgtc cgcgatggcg ggcaaatcaa cgcacgaagt gaaaacctgg gttgccgcct
     4861 ttgccgccat ttctgctttt ggcaactggc gcagcgaagg ccgttattac cgcccgatcc
     4921 cggagtggat cgccggattc ggctccctga gcgccactac acagaactaa aacagaactg
     4981 aatatgcagg agaacatcat gacttaccaa ccgcaaaccg aagccgctac cagtcgtttt
     5041 ctggaagtgc aggaagcagg cgaaacccta cgcgtgcatt tcaatgactg tgggcagggc
     5101 gatgagaccg tcgtgctgtt gcatggctct ggtcctggcg cgacgggctg ggcaaacttc
     5161 agccgtaata tcgatccgtt ggtacaggcc ggataccggg tgatcctgct ggattgtcct
     5221 ggctggggca aaagcgatgg gatcgttaac cgcggttcgc gatccgatct caacgcccgc
     5281 atcctgaaaa gcgtggtcga tcagttggat attcaaacag tccacctgct gggtaactcg
     5341 atgggcggtc acagtgctgt ggcgtttacc ctgagctggc cggagcgcgt gggcaaactg
     5401 gtattaatgg gcggcggaac gggcggcatg agcctgttta ccccgatgcc gaccgaaggt
     5461 atcaagcgcc tgaatcagct gtatcgtgag ccgaccatcg agaacctgaa gctgatgatg
     5521 gagatctttg tcttcgacac cagcgacctg accgaggcgt tgtttaaggc gcgcctgaat
     5581 aacatgctgt cgcgtcgcga tcacctggaa aactttgtcc agagtctgga ggccaacccg
     5641 aaacagttcc cggatttcag cccgcgtctg ggtgaaatca aagcccagac cctgattgtc
     5701 tggggacgca atgaccgctt tgtgccgatg gatgccggtc tgcgcctgct ttctgggatt
     5761 gccggttctg aactgcacat ctaccgcgac tgcgggcact gggcgcagtg ggaacatgcg
     5821 gatgccttta accaactggt gctgaatttc ctcacacgcc cttaaggaac ggacatgacg
     5881 aatcgtaatc ttgagcagct agccgccgat ttacgtcagg ctgaagaaaa aggggaagcc
     5941 atcgccccac tgcgtgaatt gattggtgtg gataacgccg aagcggccta cgccattcag
     6001 cgcatcaacg tgcagtacga cgtggcgcgc ggccgtcgcg tggtcgggcg caaagtgggg
     6061 ctgacgcacc cgaaagtgca gcaacagctg ggtgtcgatc aaccggactt cggtacgctg
     6121 tttgctgata tgtgttatgg cgataacgaa accatcccgt tttcccgtgt gctgcaaccg
     6181 cgtattgaag ccgaaattgc gctggtgctg aaccgcgatc tgcccaatac cgacaccact
     6241 tttgacgagc tttacaacgc catagagtgg gtacttcccg cgctggaagt ggtcggctgc
     6301 cgtatccgcg actggtcgat caaatttgtt gataccgtgg cggataacgc ctcctgtggc
     6361 gtgtatgtgg tcggcggtcc ggcacagcgt ccggcaggat tagatctgaa aaactgcgcg
     6421 atgaaaatga cgcgcaacaa cgaagaggtc tccagcgggc gcggcagcga atgcctggga
     6481 catccgatca acgccgccgt ctggctggcg cgcaaaatgg ccagcctcgg cgagccgctg
     6541 cgtgccggag acattattct caccggagcg ttaggtccga tggtggcggt gaatgcgggc
     6601 gatcgtttcg aagcccatat cgacggtatt ggttcagtcg ccgccacctt ttcagcggcg
     6661 gttacaaaag gaagtcagtc atgagtaagc gtaaagccgc cattatcggc tccggcaaca
     6721 tcggtaccga tttgatgata aaaattctgc gtcacggcgt gcatctggaa atggctgtga
     6781 tggttggtat cgatccagaa tctgatggcc tggcgcgtgc tcgacgcatg ggcgttgcca
     6841 ccacccatga aggggtgaca gggcttatcg ccatgccgga attcgccgat atcgacatcg
     6901 tgtttgacgc caccagtgca ggcgcgcatg tgaagaacga cgccgcgcta cgtgccgtta
     6961 aacctggcat ccgcctgatc gacctgacgc ccgcggcaat cggtccttac tgcgtgccgg
     7021 tggtgaatct ggcagccaac gttgatcagc agaacgtcaa tatggtgacc tgcggcggac
     7081 aggcgaccat cccaatggtg gcggcagttt ctcgcgtggc aaaagtccat tacgccgaga
     7141 ttatcgcctc gatcgccagt aaatcggcgg ggccgggtac ccgcgccaat atcgacgagt
     7201 ttaccgaaac tacctcgaag gcgattgaag tggtcggcgg cgcggcaaaa ggcaaagcca
     7261 ttattgtcct taacccggcc gaaccgccgc tgatgatgcg tgacacggtg tatgtgctga
     7321 gcgaagcggc ctctgaggtt gaagttgaag cgtcgattaa tgagatggcg gtggcggtac
     7381 aggcctacgt gccgggctat cgcctgaaac agcgtgtcca gtttgaagtc atcccggaag
     7441 ataaaccggt gaatctgccg ggtatcggcc agttctctgg gctgaaaacc gcggtgtatc
     7501 tggaagtgga aggcgcggcg cactatttac ccgcctacgc gggcaacctc gacattatga
     7561 cctccagcgc gctggcgacc gctgagaaga tggcgcagtc cctcgctcgt caggcaggag
     7621 aagcagcatg aacggtaaaa aactctatat ctctgacgtg accctgcgcg acggtatgca
     7681 cgctattcgt catcaatatt cgctggagaa tgtgcgccag atagccaaag cgctggacga
     7741 cgcacgggtt gattctattg aagtggccca cggcgacggt ttacaaggtt ccagcttcaa
     7801 ctacggcttt ggtgcacaca gcgacctgga gtggattgaa gcggcggcgg acgtggtgaa
     7861 gcatgccaaa attgccacgc tgttgctgcc gggtatcggc actattcatg acctgaaaaa
     7921 tgcatatcag gccggcgcac gggtagtgcg cgtggcgacc cactgcactg aagcggatgt
     7981 gtcggcccag catattcagt atgcgcgtga gcttggcatg gataccgtcg gttttctgat
     8041 gatgagccat atgaccacac cggagaacct ggcacagcag gcgaagctga tggaaaacta
     8101 cggcgcgacc tgtatttatg tggtcgattc cggcggcgca atgaacatga gcgatattcg
     8161 cgcccgcttc cgcgcactga aagcggtgct gaagcctgaa acagaaaccg gaatgcatgc
     8221 gcatcacaac ctgagtcttg gggtggcgaa ctcgattgcc gcagtagaag aggggtgtga
     8281 ccgtgtcgac gccagcctgg cagggatggg cgcaggcgcg ggcaacgcac cattggaggt
     8341 atttatcgcc gctgcggata agcttggttg gcagcacgga accgatctct acgcactgat
     8401 ggacgccgcc gacgatttag tgcgtccact acaagaccgc ccggtgcggg tggatcgtga
     8461 aacgctggcg ctgggctatg ccggggtgta ctccagtttt ctgcgtcatt gcgaagtggc
     8521 ggcagcacgt tatggcctga gcgcagtaga tattctggtg gaactgggca aacgtcggat
     8581 ggtgggcggc caggaagata tgatcgtcga cgtagcgctg gacctgcgca accgcaaata
     8641 aaaaatgatt acgcggctgg caacgtatca gccgcgattc acctgcatcg tgacctcaga
     8701 gggacatctt atgacgaccc gtacctccgc atcttaccgc ctgacgttga ccgttgggct
     8761 ttgcttttta gttgccctga tggaagggct ggatttacag gccgccggta tcgctgccgt
     8821 cggtatcgcc cacgcgtttg cgctggataa aatgcagatg ggctggatct tcagtgcggg
     8881 cattctcggg ctgctgcccg gcgcgctggt gggcggcatg ctggccgacc gctatggccg
     8941 caagcgtatt ctcattgcct ctgtggcgct gttcggcctg ttctcgctgg cgacggcgat
     9001 ttcctgggat ttctcatcgc tggtgtttgc ccgtctgatg accggcgtcg ggctgggcgc
     9061 ggcgttgccg aatctgatcg cgctgacttc cgaggcggcg ggcccgcgtt ttcgcggtac
     9121 ggcggtgagc ctgatgtact gcggtgtgcc gattggcgcg gcgctggcgg cagcgctcgg
     9181 gtttagcggc ctggcctccg cctggcaaac ggtgttctgg gtaggcgggg tgatcccgct
     9241 gctgctggtg ccgctgttga tgcgctggct gccggaatct caggtttata cccaacaaac
     9301 gcaggtggcc gccgcaccgt tgcgcgccct gtttgcgcca gcgacggcga gtgccacgct
     9361 gctgctgtgg ttgtgctatt tcttcaccct gctggtggtg tatatgctga tcaactggct
     9421 gccgatgctg ctggtggagc agggctttcg gccatcgcag gcggcaggcg taatgtttgc
     9481 tctgcaaatt ggcgcggcca gcggcacgtt aattctcggg gcgttaatgg ataaactgcg
     9541 cccggtggtg atgtcgctgc tgatctacac cgggatgttg gcttcactgc tggcgctggg
     9601 tacggtgtcg tcattgagcg gcatgctaat ggcgggattt gtggcgggga tgttcgccac
     9661 cggcgggcaa agtgtgttgt atgcgctggc gccgctgttt tacagcacgc agatccgcgc
     9721 cacaggcgtg ggaaccgccg tcgcagtcgg gcgtctgggg gcgatgagcg gtccgctgct
     9781 ggcagggaaa atgttagcgc tcggtaccgg aacggtaggg gtgatggcgg cttctgcgcc
     9841 gggcattttg ctggcaggcc tggcggtatt tattctgatg agccgcaaat cacagatgcc
     9901 gatcccggcc agcggtgctt aagcattcat ccggttgtgg gagtggggag atttgattcc
     9961 cacttccggg ttaacgccgt cagtcaactg tggtgccaaa gtttagataa aacatggcaa
    10021 cggaggcgtt atgagctggc ggcccttttt gtatctgatt attaatcccc acccgctatt
    10081 aagcgcccgg cgcgggcatc tgcgtctggt gcagggttga ctttgcattc tgttaacaaa
    10141 cgcggtataa caaaccttct ttggatgttt agatgtccat acgtttagaa ggttatatgc
    10201 aaacaacaca acaaaatgcg ccactgaagc gcacaatgaa aacgcgtcac ctgattatgc
    10261 tttccttggg cggcgtgatt ggcacaggat tattcttcaa taccgggtac atcatttcca
    10321 ccactggagc ggcgggaacg ctgctggcct atctgattgg tgcgctggtg gtctggctgg
    10381 ttatgcagtg tctgggcgag ctgtcggtcg cgatgccgga gaccggagcg tttcacgttt
    10441 atgccgcgcg ctatcttggt ccggctaccg ggtataccgt ggcctggctt tactggctga
    10501 cctggaccgt ggcgctgggt tcgagcttta ccgccgctgg attctgtatg cagtactggt
    10561 ttccacaggt gccggtatgg gtctggtgcg tggtgttctg cgcgattatt tttggtctga
    10621 atgttatctc cacgcgcttt tttgccgaag gggagttctg gttctcgctg gtcaaagtgg
    10681 tcactatcat cgcctttatc atcctcggtg gggcggcgat tttcggcttt attccgatgc
    10741 aggatggctc gcccgcgccg gggctgagta atatcacggc agaaggctgg ttcccgcacg
    10801 gtggcttacc gattttgatg actatggtgg cagtgaactt tgctttttcg ggtaccgagc
    10861 ttatcggcat tgccgccggt gaaacggaaa acccgcgcaa agttatcccg gtagcgattc
    10921 gtactaccat cgcgcgactg attattttct ttatcggcac cgtgtttgtg ctggcagcgc
    10981 tgatcccgat gcagcaggcg ggcgtggaga aaagcccgtt tgtgctggta tttgagaaag
    11041 tagggatccc gtacgccgct gatattttta acttcgtgat cctgacggct attctttctg
    11101 cagcgaactc cgggttatat gcctccgggc gcatgctgtg gtcgttgtcg aatgaacgta
    11161 cgctaccggc ctgttttgcg cgagtaacga aaaacggcgt gccactgacg gcgctgtcgg
    11221 tcagtatgct cggtggtgtg ctggcgctgt tttccagcgt ggtggccccg gacacggtat
    11281 ttgttgcgct gtcggcaatc tccgggtttg cggtggtagc ggtgtggctg agtatctgcg
    11341 cctcgcattt tgtttttcgt cgccgtcatc tgcaacaagg taaggcattg agtgaattac
    11401 attatcgcgc gccgtggtat ccgctggtgc cagtattagg ttttgtgctg tgcctggtgg
    11461 cctgtgttgg gctggcattc gatccagcgc agagaattgc gttgtggtgc gggttaccgt
    11521 ttgttgcgtt gtgctatggt gcttatttcc ttactcaacc ccgaaacgca aaacaggagc
    11581 cagaacatgt cgcagaataa tccgttacgc gctcttcttg ataaacagga tatcctgctg
    11641 ctggatggcg cgatggcgac ggagctggaa gcgcgagggt gtaacttagc cgacagcctg
    11701 tggtcagcca aagtgctggt agaaaacccg gagcttatcc gcgaagtgca tcttgattac
    11761 taccgggcgg gggcgcaatg cgcgatcact gccagctatc aggcgacgcc ggcgggcttc
    11821 gccgcgcgag gtctggatga agcgcagtcg aaagcgctga ttggcaaaag cgtggagctg
    11881 gcgcgtaaag cccgtgaagc gtatctggca gagaacccgc aggcgggaac gcttctggtg
    11941 gcgggatccg tcgggcctta cggcgcgtat ctggcggatg gctctgaata ccgtggcgat
    12001 tatcattgta gcgttgaggc atttcaggcg tttcatcgcc cgcgcgtgga agccttgctg
    12061 gatgccgggg ccgatctgct ggcctgcgaa accctgccga atttttccga gattgaggcg
    12121 ttggccgagc tgttgaccgc atatccgcgt gcgcgggcgt ggttctcatt taccctgcgc
    12181 gacagcgaac acctgagcga cggtacgccg ctgcgtgacg tggttgcgtt gctggcgggt
    12241 tatccgcagg tggtggcgct aggcattaac tgtattgcgc tggaaaacac caccgctgcg
    12301 ttgcagcatt tacacggttt aacggtgctg ccgctggtgg tgtatccgaa ctcgggcgag
    12361 cattacgatg ccgtgagcaa aacctggcat catcacggtg aacattgcgc gcagctggcg
    12421 gattatctgc cgcagtggca ggccgctggc gcacggttga ttggcgggtg ctgtcgcacc
    12481 acgcctgcgg atatcgccgc gttaaaagcg cgaagctgag ggtttatcgg gtctacatcg
    12541 ttcattgtag gcctgataag cgcagcgcat caggcattgc cggatggcgg cacaagcgcc
    12601 ttatccggcc tacaaaacca caaaacgtta tgccgcatcc gccagaacaa acatgccgta
    12661 cggatggatt tcaagataat actgctcgcc gacgtccggt tgcagacgcg tagcgttgac
    12721 ctgcaataat atctcctgcc cgtgccattc caccgtcact tcatactgcg gccccatata
    12781 ggcgacatgg cggatcacgc agcgctggct ctcttcgccg cgatcgctga gcgtgatcgc
    12841 ttccgggcgc acaccgacca tcccttcacc ctgtgtacca aagtgcagcg ggcgcggcag
    12901 atgatagccg tagatatcaa cgtatccgtc gctgaaggtt gccgggaaca ggttggcatc
    12961 gcccataaag ctcgccataa agcgggaggc gggctggcgg taaagatcct gcggtgagcc
    13021 gatctgcatg atgtgtccct tgttcatcac cagcacagta tcagaaaccg caaaggcttc
    13081 gctctgatcg tgggtgacgt acagcgaggt gatatcaaac tgcttttgca actcgcggat
    13141 cttgtcgcgc atgctgcgac gcaggttggc gtcgaggtta ctcaacggtt catcaaacag
    13201 cagcactttc ggcttgagga tcagcgcgcg ggccagcgcc acgcgctgct gctgcccgcc
    13261 ggagatctga tcgacaaagc ggtcttcgaa tccttccaga tccaccatcg ccaacgcctc
    13321 tttgacgcgg gctttcagct ctgcgcgcgg tacgccgagc attttcaggc cataaccgac
    13381 attctctccc agcgacatat gcgggaacag ggcataggac tgaaacacca tacagatatc
    13441 gcgctgctga atagagcgat gggtgacgtc ttcgccatca atgaaaattt gcccttcgct
    13501 cggtttttcc agcccggcaa ccaggcgcaa aatagtggtt ttgccgcagc cggacgggcc
    13561 gagcagcgtc accatttgcc cctgcgggat ggtgaggttg atattgtcga ttaccgtatt
    13621 actgccaaat cgtttagtga cgttgcgcag ttcaacgaaa tttttctgag tcatagtgcg
    13681 ctccattacg cctggttttt ggcttttgaa cgggaggtac gtgattcacc gatcagccag
    13741 tcaaagatga aaataatcgc cagcatcacc acgatcagaa tggaaccgta ggcaatcgct
    13801 acaccgtatt cgccatcttc cacgcggttg aggatgtaag ccgtcgctac gcggatatcc
    13861 ggcgtgacga ggaacacaat ggcgctgacg gtggtaatgg cgcgcacaaa gctgtaaatc
    13921 agcgccgaga ggatcgccgg gcgcagcagc ggcagcagga tgtgcgtaat ggtacgcagg
    13981 gaaccggcgc gcaggctgag tgaggcttca tcgagcgatt tatcgattt
//
LOCUS       DAJSKJ010000104        14006 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000104 DAJSKJ010000000
VERSION     DAJSKJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.104"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(320..1207)
                     /gene="rarD"
                     /locus_tag="ON345_002587"
     CDS             complement(320..1207)
                     /gene="rarD"
                     /locus_tag="ON345_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR3072630.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPANEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIVVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1259..1726)
                     /locus_tag="ON345_002588"
     CDS             complement(1259..1726)
                     /locus_tag="ON345_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR3072631.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1891..2760
                     /gene="pldA"
                     /locus_tag="ON345_002589"
     CDS             1891..2760
                     /gene="pldA"
                     /locus_tag="ON345_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR3072632.1"
                     /translation="MRTLQGWLLSVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2893..4722
                     /gene="recQ"
                     /locus_tag="ON345_002590"
     CDS             2893..4722
                     /gene="recQ"
                     /locus_tag="ON345_002590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR3072633.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4786..5406
                     /gene="rhtC"
                     /locus_tag="ON345_002591"
     CDS             4786..5406
                     /gene="rhtC"
                     /locus_tag="ON345_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR3072634.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVFAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5468..6088)
                     /gene="rhtB"
                     /locus_tag="ON345_002592"
     CDS             complement(5468..6088)
                     /gene="rhtB"
                     /locus_tag="ON345_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR3072635.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6199..7221
                     /gene="pldB"
                     /locus_tag="ON345_002593"
     CDS             6199..7221
                     /gene="pldB"
                     /locus_tag="ON345_002593"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR3072636.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFCNRHNSPSG
                     NRSTEV"
     gene            7229..8029
                     /gene="yigL"
                     /locus_tag="ON345_002594"
                     /pseudo
     CDS             7229..8029
                     /gene="yigL"
                     /locus_tag="ON345_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
     gene            8105..9004
                     /gene="bioP"
                     /locus_tag="ON345_002595"
     CDS             8105..9004
                     /gene="bioP"
                     /locus_tag="ON345_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR3072637.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8892..9845)
                     /gene="metR"
                     /locus_tag="ON345_002596"
     CDS             complement(8892..9845)
                     /gene="metR"
                     /locus_tag="ON345_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR3072638.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10081..12342
                     /gene="metE"
                     /locus_tag="ON345_002597"
     CDS             10081..12342
                     /gene="metE"
                     /locus_tag="ON345_002597"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR3072639.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12627..12872)
                     /locus_tag="ON345_002598"
     CDS             complement(12627..12872)
                     /locus_tag="ON345_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3072640.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12862..13841)
                     /gene="hcp"
                     /locus_tag="ON345_002599"
                     /pseudo
     CDS             complement(12862..13841)
                     /gene="hcp"
                     /locus_tag="ON345_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         3351 a         3469 c         3667 g         3519 t
ORIGIN      
        1 ttcttgggta aagaacctta cagcaaaaaa tctgattttg ttaaggcatg gttattgtgg
       61 tgggctatta ccatccttgt tctatttatt ttaagcctga ctacgacatt aaacttaaga
      121 agtataggta tagggttaag ttttgggttt tgtcaaatcc ttggtagata tacgatgtct
      181 ctaggatgga gtaagtactg gggagttttg gggggatttc ctgtaacaaa tattgttctt
      241 tttcttatct taattttttt caaaaaaaga taaataagtc agtgggtagg ctctttaaac
      301 ggcctaccca tgttaattct taactttttc tacgctgcgt ataaatcgca tccatcacaa
      361 aaattgccag cgccacccaa ataaaggcga aggtcaccat cttatcggca cccggttttt
      421 caccataaaa cgtcactgcc aacaggaaca tcagcgtcgg gccaatatac tggaagaagc
      481 ctaacgtgga gagacgcagg cgtgtagcag ctgcggtaaa acacaacagc ggtacggtgg
      541 tgacaatacc ggcggcgatc agcagcaaat tcagcaacat cgggttttgc cccatatggc
      601 tggttgagct gtcggcaata gcaaacagat aaatcgccgc cacgggcagc agccacatgg
      661 tttcgattaa catgccggtt tgcgcttcaa cgacaatctt cttgcgtacc agaccgtaga
      721 aggcaaaact aaatgccagt cccagcgcga taataggtag cgaaccaaaa gtccacagct
      781 ggactaacac gccacatatc gccagaatca ccgccagcca ttgcatccgg cggaatcgct
      841 cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg gttaataaag taaccaaggc
      901 tcgcttccag catatggtga ttgttcaccg cccagataaa cagtagccag ttgccaccaa
      961 tcagcacggc agagactgcc agcataaaaa ttttctgtgg cgtctgaatc agcgttttta
     1021 aataggacca ctggcggcaa atgctcatca gcaccaccat aaagaaaaac gaccagatca
     1081 cgcgatgcgt caggatctca ttggcgggca cgtagtaaat cagcttgaag tacgctggcg
     1141 caatacccca aataaaataa gcggcaagag cgagtaatac gccctgccgc gtttgttttg
     1201 catccatcgg gaatactcat ttttaattgg taacagcagt ttacctgctt ttacgttatc
     1261 aacctaccat ataggtggcg gtggcactgg caatataaag ctgttcttca ttgtgtaatt
     1321 caacgcgggc gacggcgact ttattgcctg cacgcaacag actactggta gcagtaaaac
     1381 gctcgcccct gcctgggcgc agataatcaa cgcgaagatc aatggtcccc atccgcgata
     1441 gccgctggcg tagttcatct tcactgatgg tttcgtggcg ggttaaggta cttcccacgc
     1501 acaccagacc tgcggcgaca tccagcgccg acgcaatgac cccgccgtgc aaaatgcttt
     1561 gcgcccagtt acccaccatc atcggctgat ttttaaaggc cagctgtgcg aactcttttt
     1621 cgtaacgctc cagctccatc cctaacgcgc ggttaaatgg catgtgataa acaaacatct
     1681 cacccactaa tttcagggct tgttcagcgg tcagtacggc agacatatca tccttacact
     1741 tcattggtta atgaaatgtt gattttatgc ttctttgttg ttggtttcta ctttaggaag
     1801 ggataactaa cggcaatgga gttaagtatg taaaatagcc cgcagaaaaa cattcacctt
     1861 atcaataatt cgttacggag aacacgaccg atgcggactc tgcagggctg gttgttgtcg
     1921 gtgtttatgt tgcctatggc agtatatgca caagaggcaa cggtgaaaga ggtgcatgac
     1981 gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc aggagcatga caatccgttc
     2041 acgctctatc cttatgacac caactacctc atctacaccc aaaccagcga tctgaacaaa
     2101 gaagcgattg ccagttacga ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag
     2161 ttgagcctgg catttccact gtggcgtggg attttaggcc cgaactcggt gttgggtgcg
     2221 tcttatacgc aaaaatcctg gtggcaactg tccaatagcg aagagtcttc accgtttcgt
     2281 gaaaccaact acgaaccgca attgttcctc ggttttgcca ccgattaccg ttttgcaggt
     2341 tggacgctgc gcgatgtgga gatggggtat aaccacgact ctaacgggcg ttccgacccg
     2401 acctcccgca gctggaaccg cctttatact cgcctgatgg cagaaaacgg taactggctg
     2461 gtagaagtga agccgtggta tgtggtgggt aatactgacg ataacccgga tatcaccaaa
     2521 tatatgggtt actaccagct taaaatcggc tatcacctcg gtgatgcggt gttgagtgcg
     2581 aaaggacagt acaactggaa taccggttac ggcggcgcgg agttaggctt aagttacccg
     2641 atcaccaaac atgtgcgcct ttatactcag gtttacagcg gctatggcga atcgctcatc
     2701 gactataact tcaaccagac ccgtgtcggt gtaggagtta tgctaaacga cttgttttga
     2761 tgaacggttg agtggttggc aaatctggaa cccagcatcc aggattaccc tctcagagac
     2821 taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca
     2881 atcggggtga atgtggcgca ggcggaagtg ttgaatctgg agtccggagc taaacaggtt
     2941 ttacaagaaa cctttggcta ccaacagttt cgccccggcc aggaagaaat tatcgacact
     3001 gtgctttccg gtcgcgattg cctggtcgtc atgcccaccg gtggcggaaa atccctttgc
     3061 tatcaaattc ctgccttatt gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg
     3121 ttgatgaaag atcaggtgga tcaactgcaa gccaacggcg tggcggcggc gtgccttaac
     3181 tcgacgcaaa cccgcgagca gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt
     3241 cgtctgcttt atatcgcccc ggaacgcctg atgctggata actttcttga gcatctggcg
     3301 cactggaatc cggtgttatt agccgttgat gaagcgcact gtatctccca atggggccac
     3361 gatttccgcc cggaatatgc cgcgcttggt cagttacgcc agcgtttccc gaccctgccg
     3421 tttatggcgc tgactgccac agccgacgat accacgcgcc aggatatcgt gcgcttgctg
     3481 gggctgaacg atccgctgat tcaaatcagc agttttgacc gtccgaatat tcgctacatg
     3541 ctgatggaga agttcaaacc gctcgatcag ttgatgcgct acgtgcagga acagcgcggt
     3601 aagtcaggca ttatctactg caacagccgc gcgaaagtag aagacaccgc tgcgcgcctg
     3661 caaagcaagg gaattagcgc ggcggcctat catgctgggc tggaaaataa tgttcgcgcc
     3721 gacgtgcagg aaaaatttca gcgcgatgac ctgcaaattg tggtggcgac ggtggcgttc
     3781 ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat
     3841 atcgaatcct attatcagga aaccggtcgc gccgggcgtg atggcctgcc tgcggaagcg
     3901 atgctgtttt acgatccggc tgatatggcg tggctgcgcc gttgtctgga agagaagccg
     3961 caggggcagt tgcaggatat cgagcgccac aaactcaatg cgatgggcgc gtttgccgaa
     4021 gcgcaaactt gccgtcgtct ggtattgctg aactattttg gcgaagggcg tcaggagccg
     4081 tgcgggaact gcgatatctg cctcgatccg ccgaaacagt acgacggttc aaccgatgct
     4141 cagattgccc tttccaccat tggtcgtgtg aatcagcggt ttgggatggg ttatgtggtg
     4201 gaagtgattc gtggtgctaa taaccagcgt atccgcgact atggtcatga caaactgaaa
     4261 gtctatggca tgggccgtga taaaagccat gaacattggg tgagcgtgat ccgccagctg
     4321 attcacctcg gcctggtgac gcaaaatatt gcccagcatt ctgccctaca actgacagag
     4381 gccgcgcgcc cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg
     4441 gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca actatgatcg caaactgttc
     4501 gccaaattac gcaaactgcg taaatcgatt gccgatgaaa gtaatgtccc gccgtacgtg
     4561 gtgtttaacg acgcaacctt gattgagatg gctgaacaga tgccgatcac cgccagcgaa
     4621 atgctcagcg ttaacggcgt tgggatgcgc aagctggaac gctttggtaa accgtttatg
     4681 gcgctgattc gtgcgcatgt cgatggcgac gacgaagagt agtcagcagc ataaaaaagt
     4741 gccaggatga agactccgta aacgtttccc ccgcgagtca aatgtatgtt gatgttattt
     4801 ctcaccgtcg ccatggtgca cattgtggcg cttatgagcc ccggtcccga tttctttttt
     4861 gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga tgatgggcgt gctgggtatt
     4921 acctgcggcg ttatggtttg ggcagggatt gcgttgcttg gcctgcattt gattatcgaa
     4981 aaaatggcct ggctgcatac gctgattatg gttggcggtg gcctgtatct ctgctggatg
     5041 ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcag tttttgcacc tgcgccacag
     5101 gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa tctcgctaat
     5161 ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga taacgttggc
     5221 actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc gtggtttacc
     5281 gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag
     5341 tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt gattatttcg
     5401 cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc gcatccgaca
     5461 ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata aacaacgagc
     5521 cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga gcaatccgtt
     5581 gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg gtgacgccga
     5641 gcacgatata ctgcatcagt tgtggctgtt gcggcatgat gaattgcgga aatagcgccg
     5701 ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc tggaacaaat
     5761 gtcgacgcga ttgagtagag gccagcgatt taagatcaat tgcgccagcg gcgcgccatt
     5821 gctgtattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact tcaaacgcaa
     5881 tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata tgaatcgcca
     5941 gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg
     6001 tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc gatgtcagca
     6061 ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca
     6121 caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt ttacgtggtt
     6181 taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag agaaaacgcg
     6241 tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga tgaagcagag
     6301 tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac
     6361 cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc ggaactggcc
     6421 tacgatttgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt
     6481 tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa tgattatgtt
     6541 gatgatctgg cggcattctg gcaacaggag gtccagcccg gtccgtggcg taaacgctat
     6601 atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg ccatccaggt
     6661 gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg tatgccgtca
     6721 tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg tgatggctat
     6781 gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc
     6841 agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat tcgcgtcggt
     6901 gggccgacct accattgggt acgtgaaagt attctggctg gcgaacaggt gttagccggt
     6961 gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg cgtggtggat
     7021 aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga
     7081 ggacggccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa ggacgcaatg
     7141 cgctcagtcg cgctccacgc catcgttgat ttttgcaaca ggcataactc acccagcgga
     7201 aatcgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg atttagatgg
     7261 cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaactc tgaagctgct
     7321 caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg atgtggggca
     7381 aattcgcgat aatctggaga ttaagtctta catgattacc tccaatggtg cgcgcgttca
     7441 cgatctggat ggcaatctga tttttgctca taacctagat cgcgacattg ccagcgatct
     7501 gtttggcgta gtcaacgaca atccggacat cattactaac gtttatcgcg acgacgaatg
     7561 gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg tgttcaaata
     7621 tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt tcttcacctg
     7681 cgattcccat gaataactgc tgccgctgga gcaggcgatt aacgctcgtt ggggcgatcg
     7741 cgtcaacgtc agtttctcta ccttaacctg tctggaagtg atggcgggcg gcgtttcaaa
     7801 aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg attgtattgc
     7861 gtttggtgac gggatgaacg acgccgaaat gctgtcgatg gcggggaaag gctgcattat
     7921 gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga ttggtactaa
     7981 tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat cgttctttat
     8041 ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca aagagttttc
     8101 cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta gcttttatgg
     8161 cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg ttggcttggc
     8221 ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa cagtcggcct
     8281 gtatatgctg gtgggcgcga tgcagcttgg cgtgatgtat atgctgagtt tccgcgctta
     8341 tctctatctg acggtttccg aactgctgct gttcaccgta ctgacgccgc tctacatcac
     8401 gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggatatgctt ttagtgcctt
     8461 gttggcggtg attggtgccg ggattattcg ctatgatcag gtcaccgacc atttctggac
     8521 tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg tgggttacaa
     8581 acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt tctatcttgg
     8641 cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga aaatgccgca
     8701 aaccacgttg caatggggta ttctggtgtt tcttggcgtg gtggcttccg ggattggcta
     8761 ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggaa ttatgaataa
     8821 tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac agccgcactg
     8881 gccaacgttt attacaggcg cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg
     8941 ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga
     9001 ataaacgctt ccgtaactgg ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg
     9061 ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc aaaactctct
     9121 actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg aatcaacaat
     9181 aaggtgttat cgacgctttt tagtgacgga ctgacgcctg ccggctgaag aaaatgccgc
     9241 cagacatcca gtcgactacg ctgcaccgga taaattaata gcgtctcgct ggcgagatct
     9301 tccggtgtaa tacgcgtttt tgccgccagt ggatggtcag gtgctaacac cagacgcact
     9361 tcatagtcga acatcggcga ataatgcaga ccactgcgcg gcagaatatc ggacgtcatt
     9421 accagatcca gctctccctg ttgcaaggcg ggctgcgggt caaatgtcac gcccgattta
     9481 aagtccatct cgacctgcgg ccagttctta tggaaatttt ctaacgcggg cgtcagccac
     9541 tgtatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag
     9601 gcttgcagtg cctggctaat ttgtggcagc acctggtttg ccagttgcaa caggatttct
     9661 ccctgcggtg taaagcgaag cggctggctt ttacgcacaa atagccggaa gccaaggcgt
     9721 tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa cgtcgccgca
     9781 gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg ttttacttcg
     9841 atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat acagtaaccg
     9901 ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta aatttatggt
     9961 gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg ggatgaataa
    10021 acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag aggaagaaaa
    10081 atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa
    10141 aaagcgcaag agagttattg ggcagggaac tccacgcgtg aagaactgct ggcggtaggg
    10201 cgtgaattgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga cctgctgccg
    10261 gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct gggtaatgtt
    10321 ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt ccgtattggt
    10381 cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac
    10441 accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa actgacctgg
    10501 acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt taagcctgtg
    10561 ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca gtttgaccgc
    10621 cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga actggcgaaa
    10681 cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact accacaggcg
    10741 tggctggacg catacaaacc cgcttacgac gcgcttcagg gacaggtgaa actgctgctg
    10801 accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct gcctgttcag
    10861 gggctgcatg tggaccttgt acatggtaaa gatgacgttg ttgaactgca caagcgcctg
    10921 ccttctgact ggctgctgtc tgcgggactg atcaatggtc gtaacgtctg gcgcgccgat
    10981 cttactgaga aatatgcgca aattaaggac attgtcggca aacgtgattt gtgggtggca
    11041 tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg tcttgatgca
    11101 gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactggc actgctgcgc
    11161 gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc gattcaggcg
    11221 cgtcgtcact ctactcgtgt acataatccg gcagtagaaa agcgtctggc ggcgatcacc
    11281 gctcaggaca gtcagcgtgc gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt
    11341 tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac cacggaaatt
    11401 cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta ccgcacgggc
    11461 attgcggaac atatcaggca ggccattgtt gagcaggaac gtttgggact ggatgtgctg
    11521 gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca cctcgacgga
    11581 tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt gaagccaccg
    11641 attgtcattg gtgacattag ccgcccggca ccgattaccg tggagtgggc gaagtatgcg
    11701 caatcgctga ccgacaaacc ggtgaaaggg atgctgacgg ggccggtgac catactctgc
    11761 tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat tgcgctggcg
    11821 ctgcgtgatg aggtggccga tctggaagcg gcagggattg gtatcatcca gattgacgaa
    11881 ccggcgctgc gcgaaggttt accgctgcgt cgtagcgact gggacgccta tctccagtgg
    11941 ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca aatccacact
    12001 cacatgtgtt attgcgagtt caacgacatc atggattcca ttgcggcgct ggatgcggat
    12061 gtgattacca tcgaaacttc gcgttctgac atggagttgc tggagtcgtt cgaagagttt
    12121 gattatccga acgaaatcgg tccaggcgtc tatgacattc actcgccaaa cgtaccgagc
    12181 gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc agagcgcctg
    12241 tgggtcaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg cgcagcactg
    12301 gcgaatatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt aacgagtcac
    12361 tgtaaattgc agtacgatga aagaggcaaa actgcctgat accaggccta cataatttat
    12421 gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc gcatccggca
    12481 tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc cattttgcta
    12541 tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata taatcctgag
    12601 cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa attgtatccc
    12661 ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat aataaccaaa
    12721 ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg aatttgcctt
    12781 atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc agccataact
    12841 ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt ggtataagtc
    12901 attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgtat gattccagag
    12961 tgcatagcct gcgtcaccat caggttgtcc tgtttcccaa ccaagattac agtaacgctg
    13021 atattcccga cactcatgag tatagaagtt aagatctact tgttctgggg ggatttttcc
    13081 tttctcaatg tctttcagtc tattgatcat cacttcattt acagggtcat gaccaaatct
    13141 ggctatgtgt ttttcaacaa tagcgatacc ttccctgttg attgttacgc catctgtcgt
    13201 cagattttct attggaccac cggcaaggtc gggattaaat ggtcttcctg atactgctcc
    13261 tctggctccg aaatgctcaa gtgattccaa atcagggagt ctcattctcc ccagaactgc
    13321 tccagcaact attgaagcac cagcgatgag taaccctttg ttatccataa attgatcgta
    13381 accagcaagt gcatctcccc caacttgtct gctgtttccc tgaatccttc gatattccca
    13441 ttgtatatgc cgacagcagc aagcagtctg ccaaccgctt tactattaag tgttttgaaa
    13501 ccattaccag ctttagtttt tggccttggt ataaacagac ggttgtagtt atcgggaaat
    13561 gcccaataac taaattcagt attagcaatc agatgcctgg agagtatata ttcataactg
    13621 acagtgatgg cttctgtatc cagattgttc tcagtaaaca ggtgattgat tgctgataag
    13681 agcgcgccgc ggagttgaat gttgtaatat ttctcccatc gaccaaacct attgatccga
    13741 tagaagtcaa actccatgaa caactgctcg ttattgttaa tagcgttaat aagtaacggg
    13801 gagcttttgt caatcaattt acagaaacta aggccatgca attatgaccc tttgcctgta
    13861 ttcgtaatac tattagagag cgagaacgcg aagatttcat catcatggcc aatctgccag
    13921 cgattaccta ttgatgcgct cgttccacaa agagaggaaa tacatccttg ttgttctccg
    13981 gttatggtca agtaaataat atcact
//
LOCUS       DAJSKJ010000105        13919 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000105 DAJSKJ010000000
VERSION     DAJSKJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.105"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            224..952
                     /gene="mpaA"
                     /locus_tag="ON345_002600"
     CDS             224..952
                     /gene="mpaA"
                     /locus_tag="ON345_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR3072641.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLILHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(927..1892)
                     /gene="ycjG"
                     /locus_tag="ON345_002601"
     CDS             complement(927..1892)
                     /gene="ycjG"
                     /locus_tag="ON345_002601"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR3072642.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKTLKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2011..2517
                     /gene="tpx"
                     /locus_tag="ON345_002602"
     CDS             2011..2517
                     /gene="tpx"
                     /locus_tag="ON345_002602"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR3072643.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2561..4102)
                     /gene="tyrR"
                     /locus_tag="ON345_002603"
     CDS             complement(2561..4102)
                     /gene="tyrR"
                     /locus_tag="ON345_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR3072644.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4250..5311)
                     /locus_tag="ON345_002604"
     CDS             complement(4250..5311)
                     /locus_tag="ON345_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR3072645.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5308..6705)
                     /locus_tag="ON345_002605"
     CDS             complement(5308..6705)
                     /locus_tag="ON345_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR3072646.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLVQLYGDPPAWPAPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQVDKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6861..7859
                     /locus_tag="ON345_002606"
     CDS             6861..7859
                     /locus_tag="ON345_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3072647.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIENGHKNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(7970..8875)
                     /gene="ompG"
                     /locus_tag="ON345_002607"
     CDS             complement(7970..8875)
                     /gene="ompG"
                     /locus_tag="ON345_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR3072648.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(8920..10002)
                     /locus_tag="ON345_002608"
     CDS             complement(8920..10002)
                     /locus_tag="ON345_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3072649.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIALR"
     gene            complement(10016..10675)
                     /gene="pgmB"
                     /locus_tag="ON345_002609"
     CDS             complement(10016..10675)
                     /gene="pgmB"
                     /locus_tag="ON345_002609"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3072650.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPVIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(10672..12939)
                     /gene="ycjT"
                     /locus_tag="ON345_002610"
     CDS             complement(10672..12939)
                     /gene="ycjT"
                     /locus_tag="ON345_002610"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR3072651.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVTRSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(12936..>13919)
                     /locus_tag="ON345_002611"
     CDS             complement(12936..>13919)
                     /locus_tag="ON345_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3072652.1"
                     /translation="SYYCTRNDLELVDVCDSRLSQAQAQALAEKYGNASVWDDPQAML
                     LAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCEKPPAMTPEQAREMCDTARKLGKVL
                     AYDFHHRFVLDTQQLREQVTNGVLGEIYVTSARALRRCGVPGWGVFTNKELQGGGPLI
                     DIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIE
                     FHNGGILWLETSFALNIREQSIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQRELA
                     DDNRHLRSMEAFINHVQGKLVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
BASE COUNT         3330 a         3906 c         3364 g         3319 t
ORIGIN      
        1 taacatgtgt agaaccagta agaataaaat acgccatgta tataacatta cgacttacat
       61 ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac ttccggcaaa
      121 aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa tccgcaaaat
      181 ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg taacccgccc
      241 acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt cattattggg
      301 cgcgccgtta atctggtttc ctgcccctgc agccagccgc gaaagtggtt tgattctggc
      361 tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc tgcggacgtt
      421 gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg gctgccagct
      481 ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg cggcgaactg
      541 gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg atgtcgtatt
      601 actgacaggt gacaaacctg gctcagaacc tgaaacccag gcgctgtgcc agcttataca
      661 ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcctgta ttgaagatcc
      721 cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc tggtaaccag
      781 cgtcggttat gaaacgcctg gctctttcgg cagttggtgt gcagatctca tcttgcattg
      841 catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat atctctttgc
      901 gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt gaactgaagc
      961 gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa actgacctgc
     1021 ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca gcccagcatc
     1081 agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt cagaccaccg
     1141 gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgtctt cagattgcta
     1201 cgtgtatgac aactctcatc agcacaaatc ggcaacggat gaataaaatt ctccagcgcc
     1261 gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa cgcctaaatc cgccaatagc
     1321 tggcaacgcg ccgccaaccc ttctgcacgc caggattcat ttgcatcaac gatcagcgtc
     1381 gcatcgggca cagctgtgcg aattgccacc atccgctcac taataagatg gttatccagc
     1441 ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt ggccatctga
     1501 tcaggcgtac cgatgactac cgtctgtgca gtgataactg tctcgggaag cgttatgccg
     1561 atcaaatcag ccagcgattg ctgctgcttt cgcgcggcca gatcccacaa tgcacaatcc
     1621 agcgcattac gtgctgcgcc ggcagggaga attttttgca attcctcccg tgtcagccct
     1681 ttctctaatt gcgacacgac gctcataatt tgcgccatta ccgaggcatc actttctcca
     1741 taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc cagttcaacc
     1801 accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt atgtaagggc
     1861 caggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg cagtggtctg
     1921 cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac taggtgtgac
     1981 gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt ccagggcaac
     2041 ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac ttttactctc
     2101 gtggcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg
     2161 aacattttcc cgagtattga taccggtgtt tgcgccgcat cagtacgtaa gtttaaccaa
     2221 ctggcaaccg agatcgacaa caccgttgtg ctgtgtatct ctgccgatct gccgttcgcc
     2281 cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc cactttccgt
     2341 aacgctgaat ttctgcaagc ttacggtgtg gcaattgctg atggcccact gaaaggtctg
     2401 gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag ccagctggtg
     2461 gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa agcataattt
     2521 aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg ttcttcttct
     2581 gactcagacc atattcccgc aacttattgg caatcgcggt atgtgaaacg ccgagacgtt
     2641 ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc gagcgttcaa
     2701 aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg
     2761 cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg tccagttgtg
     2821 tcagtgcgcg atagatagcg ttctttaact gccgcacatt tcccggccac gcataacgtg
     2881 taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc tcgtcggcaa
     2941 agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca cgtagcggcg
     3001 gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac acgccttttt
     3061 gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg acatgcacct
     3121 catggtcttc gccaacccga cggaaagtgc catcattaag gaaacgcagt aatttcgcct
     3181 gcatccgtgg tgacatttcc cctatttcat ccaacagcac cgaaccaccg ttcgcctgtt
     3241 caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg accgcatctt
     3301 ccggtataga cgcacagttc agcgccaggt aaggtttgcc cgctctgggg cttgcctgat
     3361 ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc acccgtaatc agcagcggcg
     3421 cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc gggctgacgg
     3481 cgacaatttg actgaaggcg ctgacgtcct gggcggcgac attttgcaac tggcggccca
     3541 tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga tcattttcat
     3601 cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata acgacatgct
     3661 cgttatgcga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag ccgttaatca
     3721 attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg
     3781 ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt tcaggcaacg
     3841 cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac gggacagtac
     3901 gcacatcggt aacacccgca atacggcgta tttcggccat cagactgctg aaactctcaa
     3961 actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca ataccgcgta
     4021 aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg agtcggtctt
     4081 cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat taatatgatg
     4141 atatgacact atttggatag caggaagttt tctgtcaaca atcattgaca caaagacgga
     4201 aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat tatttttcgc
     4261 tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga cgacggaaat
     4321 ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt
     4381 taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg cgggtagaca
     4441 aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc agttcgctgg
     4501 ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg ctgtaatacc
     4561 ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg cgccaggcga
     4621 taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt gattccgccg
     4681 ccgaacggct gatttcgcgc cgcgcctggg catctaaaac tggctggacc aaatgcgcat
     4741 acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac cagcgttgca
     4801 gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa aatgcgcggc
     4861 ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca tgggcgcgct
     4921 gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca gcgccgataa
     4981 tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc caggcattca
     5041 ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc ccgcccatca
     5101 ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc gcttcaactt
     5161 gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc gcctgatttt
     5221 cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc ggaccgtcga
     5281 aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta aaaattccag
     5341 cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct gcgggcgaaa
     5401 cgcttcaaac tggaaccctt gtttgtccca gaacgcctga ccgggcaaac gcgcaggaac
     5461 ttcaccggga taaacggtga gcggcgcacc atcgctgagc cggttaccgc gcaatgccgg
     5521 aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg aggccagccc
     5581 caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct gaatcagttg
     5641 ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc acatggtccg ctttagtggc
     5701 agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg tacgctgccc
     5761 gtagtgaaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa atgcctgtgg
     5821 cccactgttg agaggttgca ggcaatccac cagcacaatc tggcggtcaa agcgcagaaa
     5881 atgattctta tagaacccct tcaccacctt ctcgcaataa taattaaagc gctcgcgcag
     5941 cattccggca ttggtgtgct tatcgacctg cgccagtttg gactcgcccc aggcatcgac
     6001 atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatcccccg gcaagacaaa
     6061 gcgcccaggc tgaataaagt gcagcccctg ctgtttacag tggtggagat aatcggtcca
     6121 cgcggcggca atgtctgcca gccggttttc gtcggcaggc gctagtgggt ccagcccttc
     6181 gcacatcatt cgccatttcg ccgaccattc gccgcgctga ccattgagta agcccgtcat
     6241 ctggcgcgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca accattcgcc
     6301 ggggtaatcc acaatctcca gatacagcgt ggaggtatcc ttaaagtggc gtagcagcga
     6361 atcgttcgat ttaaaacgta acgccaggcg aatttcactg acgccgcgcg taggcgccgg
     6421 ccaggcggga ggatcgccat ataactgcac cagtccttca tcataggtga agcgcggaat
     6481 gccaaagtca cgctgaggaa tgcgttttac gcccagcaga cgctcttcac gcaccgcgct
     6541 taacagcggc aaacgtgctc cggcatgaat attaagcaac tgattaacca tcgcggtgat
     6601 aaacgctgtt ttgccgctgc ggctaagtcc ggttacagcg aggcgcagat gtctgtcgac
     6661 accccgattc accagcgcat tgagttcatt tttaagtcgc ttcattgctg tcctgtgctg
     6721 ctctgaagac cagaaaaaaa atcattcgta aggatatgaa tgtgggggcc aggccattga
     6781 ttatcaactg cccgtgttgt catcgacggg cgaactccat aagatgtgca gcattcacgt
     6841 tcggacggag ttcctgacct atgtccccta ctatttatga tattgccagg gttgcaggcg
     6901 tatcaaaatc caccgtatca cgcgtgctga ataagcaaac caatatctcc ccggaagcgc
     6961 gcgaaaaagt gttacgggcc attgaagaat tacagtatca gccaaacaag ctggcccgcg
     7021 cgctgacctc ttcgggtttt gatgccatta tggtgatttc tacccgttcg accaaaacta
     7081 cggcgggtaa tccgtttttc tctgaagtgc tacatgccat caccgccaaa gcggaagaag
     7141 aaggtttcga cgtgatattg cagacgtcgc acaacctggc agaagactta caaaaatgtg
     7201 aaagcaaaat taagcagaaa atgattaaag gcattatcat gctaagttcg ccagcggatg
     7261 agtcattttt tgcccaactc gataaatacg atattccggt cgtggtgatt ggcaaagttg
     7321 aaggtcaata tgcccatgtt tattctgtcg ataccgataa ttttggtgac agcattgcgc
     7381 taaccgatgc attaattgag aatggacata aaaacattgc ctgcctgcat gcgccgcttg
     7441 atgtccatgt ttcagtggat cgggtaaatg gttataagca aagtctgtcc gcgcataata
     7501 ttgcagtgcg tgatgaatgg attgttgacg gcggttatac ccatgaaaca gcactgcaag
     7561 ccgcgcggga attattaagc cagtcaccgt tgcctgaggc agtgtttgct actgacagcc
     7621 tgaaattaat gagtatttat cgtgcggcag cagagaaaaa tattgctatt ccgcagcagt
     7681 tagcggtggt gggttatagt aatgaaacgc tgtcgtttat tttaacgcct gcgccaggcg
     7741 gtattgatat cccaacacag gagttaggtc aacaaagctg tgagttatta ttccgcttaa
     7801 tttctggaaa accgtcacca cagaatatta ccgttgccac gcatatgacg ctgaaataaa
     7861 taccagctac cttgcctgaa tgccggatgc gatgctagcg catcttatcc ggcctacatt
     7921 tatagttcgg ataaagtact cgtgccgcat ccggcaattt agcccattat cagaacgagt
     7981 aatttacgcc gacacctgca taatggaatt tatcgctgtc gccttcgtcg tgatcctgcc
     8041 actcaaacgc gtattccagc gaaacggaaa gcccgttctg gaaatcataa ccgtaaaata
     8101 aacctacacg gttaaaatca tggccttcac gttcaatatc atcctgccag tcccagttac
     8161 tccagcgatc cagcccaatg cgcgtatacg gcgtcaccga gtggttgccg agcgtcagcg
     8221 gcaaataggc gcgaatttct tgcgtagaaa actcaccgtt attgcggctg tcgtccatat
     8281 taaagccgcg ctcgagataa tagttcactc gcaaggcaac cgtttcgttg aaggtatatt
     8341 gcaggcctgt ttccgtttcg actcgggtat cagcgtaacc ggtcgtgttc agatcgttgg
     8401 caaatttata catcgataac caaccgttga aacgtaaatc gtccgtcagt ttcacatccc
     8461 agtctggcgc gattttccag cgctgcatat tcgccgtgtc tttacccggt tcatcaacgt
     8521 agtgataacc ataattacgg aaaccaccgg tcaggccgaa actgaaatca tcgttttcga
     8581 ggaactgata atgcacctcc agctccgggc gatcaaacca cgttccacgt ttacccgcgc
     8641 tataatctac cggcccttcc tgataatagg ccagagcaat tctccacggc ccgttggcgg
     8701 cattaaaata gactgaaggc tccgccagcc catccatatc ttcgccataa ccctcgacgt
     8761 tttctatttc gtacatcgcg ccgatattaa agtgccagtc gttcttttcc tcggcctgtg
     8821 cgcaggccat tcccgcacac atcaccagtg cggtacaggg taataacttt ttcattattg
     8881 ttccttgtat tatcctgggg gaatgccttc cccctgtatt tagcgaagtg ctatttccgt
     8941 ttctgcatca aagaaatgac atttagtcat atcaaaatga atagtgatat tttctcctgc
     9001 atgataatca tttaacgcac cagcacggac cactaactcg tgccccccaa ccgtggtgta
     9061 gagcataaat tccgcaccgg ttaattctgc cacgctaatt ttggcggaaa tgttattttc
     9121 ctcttgcgcg tccggatgaa tatcttccgg gcgtattccc atcacgatgg gcttatgcaa
     9181 actttcctgt gttttcagaa ccgctaattt ctcttcggga atggttaatt taagcgtttc
     9241 cgtaacgaat ttatcgccat cgatcgtgcc acgaataaaa ttcatcgccg gtgatccaat
     9301 aaatccggca acaaacatat tcgctggttg gttataaacc gttttcggcg caccaacttg
     9361 ctgaacaatc ccatccttca taatcacaat ccgcgtcgcc atggtcatcg cttcggtctg
     9421 atcgtgggtc acgtagatca tggtggtatt cagcttctgg tgtagcttgc tgatctctgc
     9481 gcgcatttgc acgcgcagct tggcatcaag gttagagagc ggttcatcca ttaaaaacac
     9541 gcccgcttcg cgcacaatcg cccgcccgag cgccacacgc tggcgttgcc cgccggaaag
     9601 cgcgcccggc ttgcgtttca ggtactcacg caggccgaga atttgcgccg cccagttcac
     9661 ccgctcatca atcacctctt tggcgatttt ttgcatcttc aggccaaacg ccatgttgtc
     9721 gtaaaccgtc atatgcggat acaacgcgta gttctggaac accatcgcta tattgcgtgc
     9781 tttggctgga acgtcattca ttcgtttgcc gtcgatcaat agatcgccgc cgctgatctc
     9841 ctcaagcccg gcaatcatgc gcagggtggt cgacttaccg cagcccgacg ggccgacaaa
     9901 cacgatgaac tctttatcgg caatttccag gttgaagtcc ttcaccacat gcacctggtt
     9961 atcgtagatt ttttgaatat gttgtaacga aagctgagcc atgttgattc ctttgctata
    10021 cgttttgcca gaaggccgat aaccgcggcc aggtgagtga ttccgttgaa ggcaacagta
    10081 attgcgcccc ggttaagccc gcgccgatcc ccaccgagcg cataccgctg gcgttaatgg
    10141 cgtcaatgcc cgcctgcgca tcttcaatgc cgatacatgc ctgcggcggc acgcccagcc
    10201 cggcacaggc ggcgagaaag atttccgggt ccggtttcga gtttttaagt tgggaagcat
    10261 ccgcgcagaa ggtgaaaaac tcgcgcagct ccagcgccgc taaaatcgtc ggcgcattca
    10321 gggagacaga agccagccca accgggatct gctgtgcacg gagatctgcc agcaaagagc
    10381 gaatgacggg tagaacagcg ttgaccgtca actcgcgcag tgagtggaca tagagcagat
    10441 ttttgcgata cgccagttgc gccctctcct gcgagttaaa gtcgccctct ttgcccccgt
    10501 gttgcagaat gcgccgcaga gactcatcgc ggctgatccc ttttagggat tcgttaaact
    10561 gcgcatcaat gctgatgcca atttcagcgg caatctgctg ccacgcctgg aaatgcagat
    10621 gcgcggtatc ggtgattaca ccatccagat cgaaaattac cccttgcagt ttcattcatc
    10681 ctcctgatgt gtggtagcgg tcccattgaa gggcaaaata aaatcaccca aacagaaaac
    10741 agattcttca gccacggata taagctgacc gttcaaacgc agtgaaacgg gcgcagaagt
    10801 tcgaatcgca atacgctgcg cgtcaagagt gacctgtaat tcgcagccct gccagaacag
    10861 agggaaagac aactgttgcc actgctcagg taacgccgga ttgagatgca attcaccgtc
    10921 acgcacgctc accccggcaa aaccctgaat cgcccccagc cagatagcgc cagttgcggc
    10981 agcatggata ccatcatcac aactatgcgg atcagcacca agatcgattt cagtcccctc
    11041 gcgccagaac tgataacttt gggtcagcag gccacagcgt gcggcaacaa tgccgtggat
    11101 tgctttactt aatgacgagt cgtgaatagt gcgcggttca taaaattgca gattggcaag
    11161 acacgatgcc gctgagaact gctccggcag catgtaattg agcatcacca catcagcttg
    11221 tttgaggatt tgcatctcgt tcacttctgc gcgtgaataa tccagcagaa tggtttgctt
    11281 ccccgccgcc gctttgtatt tcgccagatt aatcgccggc ttagccataa acgaatcatc
    11341 ctgcggcaaa acgccgtcgg gctgaatttc tggcagccgc agttctttaa ggaacatttc
    11401 ggcgcgatgg ataaacgcat cgtcgctaca gccaaactgg cgggcaatac tcagcgcctg
    11461 ctggacgttg taatacgcca tatagctggt gaaggcgtta ttattgacat gttcggtata
    11521 ttcgtcaggc ccaataacat catgaatttc cagacggtcg ttaaccctca ccgcgcggct
    11581 aatccagaac tttgcagttt ccagaagtag cgccatgcct tcatgcgcaa tgaaactttc
    11641 atcccccgtg gtctgccagt attgaataac cgcccaggcg atatcggcca ccagatgatg
    11701 ttccgcctgc gccgaggcca ctttttgccg cagcccggtg cgaatgttaa tggcggcaaa
    11761 ttccggcgtc tcttcttcgc cgctgcgcgc gctttcccac ggaaatagcg cgccctgcca
    11821 gccgttgcgt cgcgctttct cctgcgcgcc tggcaagttg tgccagcgat aacgcagtaa
    11881 acttcgggta accgtcggat cgctaaacag atgaaacggt aacaaaaaca cttctgtatc
    11941 ccagaaaacg tggcctttgt agccttcgcc ggttaagcct tttgccgcaa tgctgctgcg
    12001 ctcgtcgtgg gcaggcgtca tgatgcgcag atgataaagc gcataatcta acgcttgctg
    12061 atcgtgcgct tcgccgccat ttaccgtgat acgacgtttc tgccaccatt gacgccagtt
    12121 ttctgttgat actgcaagaa gttggtcgta actctgctgc gcgcacattt caagctggcg
    12181 aagcgacgcg ctgccccact cgtctaaaac ggcttgcctg tcatcccgcc agtcgatcca
    12241 caccagtttt tgcaacgtca ctgtctcgcc tgcatgaagc tgcgcactgg tatgttgctg
    12301 caaacggcgc tctttggcgg tatagcattg ctgcacatca ccgctcacca tacagcaaca
    12361 gctgatggcc acatcactgc gtccatcctg ggtggtgtag atcccctgca tcagatgctg
    12421 accaaacacc cgcacctggg tttcgtcgag atgttggcga ccgtggttgg tttgcgtggc
    12481 gtcgatacct gttgaaatca gcactgacgc gtcggcgtcc agtggcgtaa tagtgatttc
    12541 cagcacaatt agcggcagtt ggtctgccga aacaaagcga cggctggcga tggtgtaacc
    12601 tgtgccgttg ctggtacgcc agacaacgct acggcgtaat tctccactgg caaagtcaag
    12661 ttcacgctgc caggcttcgc gggataacga gaaaacctca ccatttatgg caatctccat
    12721 ccccacgaca tcaggcaggt tcaccagttc gttgatttca ccttttcccg cccgatgata
    12781 cagccccgcc agatacatcc ctcgcgtctg gcgcgtgtaa tcttcttcat ggctggcgcg
    12841 aagcccaaga tagccgttcc cctgcgccat cagcgatgca tacttgttca gggtatgctg
    12901 gctgaaatgg ggttctgata acgttactgg cctggtcata attccacacg cgtccctgtt
    12961 tctgcggatt gatacaacgc cgccaccagt tgctggatga tgtacccctg ctcggcgtcg
    13021 gctatcatca cgagcttgcc ctgtacgtga ttgataaagg cttccatgct gcgcaaatgg
    13081 cggttgtcgt ctgccagttc ccgttgcatc agcgtcatta attcaccgtt gttatcggtg
    13141 tagatatgtg ctggaaacag cgtcgcaccg gctttatcac cacaaaagct gacgttcata
    13201 atcgactgtt cgcggatgtt gagtgcaaat gacgtttcca gccacagaat gccgccgtta
    13261 tgaaattcaa tggtgccaaa cagcgaatct tcgacgctgt aagttgccgg atcccactcg
    13321 ccaaattgac cacagctctt ttgcgtgccg atcttttgaa agctatgcgc attcacgctt
    13381 ttcaccgccg gaaagcccag cacatacatc gcagcatcca gcatatgaat gccgatgtcg
    13441 atcagcgggc caccaccctg cagttcttta ttggtaaaga caccccagcc gggaacgccg
    13501 cagcgacgca gggcgcgggc ggaggtaacg taaatctctc ccaaaacgcc gttggtcacc
    13561 tgttcacgca gctgttgcgt atcgagcaca aaacgatggt gaaagtcgta ggccagcacc
    13621 ttgcccagtt tgcgcgcggt atcgcacatt tcccgcgcct gttctggcgt catggcgggc
    13681 ggtttttcgc acatcacatg gcagcccgct tccagtgcca tcagggtatg ttcgtaatga
    13741 aaacggttag gtgagcagac gctaaccaca tcaggtttca ccgccagcag catggcctgc
    13801 ggatcgtccc acacggatgc attcccgtat ttttctgcca gcgcctgcgc ctgcgcctgg
    13861 gaaaggcggc tgtcacagac atccaccagt tccagatcgt tgcgggtgca gtagtacga
//
LOCUS       DAJSKJ010000106        13837 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000106 DAJSKJ010000000
VERSION     DAJSKJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.106"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..554
                     /locus_tag="ON345_002612"
     CDS             <1..554
                     /locus_tag="ON345_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR3072653.1"
                     /translation="KTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEIHQDDI
                     DKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFDSSAKT
                     TGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFFAWMEQ
                     IDQATPVTPGAVTANATYVLDYK"
     gene            605..3064
                     /locus_tag="ON345_002613"
     CDS             605..3064
                     /locus_tag="ON345_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR3072654.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGVWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWDSSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3079..3810
                     /locus_tag="ON345_002614"
     CDS             3079..3810
                     /locus_tag="ON345_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR3072655.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPTALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLSCSGQCHLQAVEAKK"
     gene            3807..4868
                     /locus_tag="ON345_002615"
     CDS             3807..4868
                     /locus_tag="ON345_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3072656.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5020..6066
                     /locus_tag="ON345_002616"
     CDS             5020..6066
                     /locus_tag="ON345_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3072657.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRFPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6063..6854)
                     /gene="nei"
                     /locus_tag="ON345_002617"
     CDS             complement(6063..6854)
                     /gene="nei"
                     /locus_tag="ON345_002617"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR3072658.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6890..7624)
                     /gene="pxpA"
                     /locus_tag="ON345_002618"
     CDS             complement(6890..7624)
                     /gene="pxpA"
                     /locus_tag="ON345_002618"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR3072659.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMHACV
                     REAIKNGVAIGAHPSFPDRENFGRSGMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7614..8546)
                     /gene="pxpC"
                     /locus_tag="ON345_002619"
     CDS             complement(7614..8546)
                     /gene="pxpC"
                     /locus_tag="ON345_002619"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR3072660.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8540..9196)
                     /gene="pxpB"
                     /locus_tag="ON345_002620"
     CDS             complement(8540..9196)
                     /gene="pxpB"
                     /locus_tag="ON345_002620"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR3072661.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9219..9962)
                     /gene="ybgI"
                     /locus_tag="ON345_002621"
     CDS             complement(9219..9962)
                     /gene="ybgI"
                     /locus_tag="ON345_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR3072662.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10233..11714
                     /gene="dtpD"
                     /locus_tag="ON345_002622"
     CDS             10233..11714
                     /gene="dtpD"
                     /locus_tag="ON345_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR3072663.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11756..13174)
                     /gene="phrB"
                     /locus_tag="ON345_002623"
     CDS             complement(11756..13174)
                     /gene="phrB"
                     /locus_tag="ON345_002623"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR3072664.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13171..13680)
                     /locus_tag="ON345_002624"
     CDS             complement(13171..13680)
                     /locus_tag="ON345_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR3072665.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         3320 a         3723 c         3399 g         3395 t
ORIGIN      
        1 aaaaaacatt ggccgcactg gccgtatctc tgctgttcac agcacctgtt tatgcggctg
       61 atgaaggatc cggtgaaatt cactttaaag gtgaagttat tgaagcaccg tgtgaaatac
      121 atcaggatga tattgataaa gaggttgaac tcggtcaggt gaccaccagc cacattaatc
      181 agtcacatca cagcgatgcc gttgctgtcg acctgcgctt agtcaactgt gatctggaaa
      241 actccagcaa cggttccggt ggcaagattt ctaaagttgc agtcaccttt gatagctcag
      301 cgaaaaccac cggcgcagat ccgattctca acaacaccag cacgggtgaa gccaccggcg
      361 tcggcgtacg tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc gctactccag
      421 atatcgacct ggctccgacc tccagcgaac aaacgctgaa tttctttgcc tggatggaac
      481 aaattgatca ggctacacct gtaacgccag gcgcagttac ggcgaatgca acctacgtgc
      541 tggattataa ataagtatta ctttggcggc gctatcgccg cctttcataa aatatttatc
      601 aggtatggac accgtgaata tttatcgact ctcttttgta tcctgcctgg tcgtggcgat
      661 gccttgcgca ttggcggtcg aattcaacct taatgttctc gataaatcaa tgcgcgaccg
      721 cattgatatt tcattattaa aggaaaaagg agtcattgct cccggtgagt attttgttag
      781 cgttgcggta aataataacc aaatcagtaa cgggcaaaag attaactggc acaaaaatga
      841 cgataaaacc attccgtgca tcaatgattt actggtcgat aaatttggct taaaacctga
      901 agtccgtcag tcgttaccat tgataaatca gtgcgtcgat tttagctccc gacctgaaat
      961 gctcttcaat ttcgatcaag ccaatcagca actgaatatt agtattccgc aagcctggct
     1021 ggcgtggcac tcagaaaact ggaccccacc ctccacatgg aaagaaggtg tcgccggtgt
     1081 cctgatggat tacaacttgt ttgccagcag ctaccgccca caggatggca gcagcagcac
     1141 taatcttaac gcctatggaa cagccggaat caacacgggg gtatggcgct tacgcagtga
     1201 ttatcagttg aatcagactg atagcgatga taaccatgaa cagtcaggcg aaatatcacg
     1261 tacctatctt tttcgcccgt taccgcaatt aggttcaaaa ttaaccctcg gcgaaacgga
     1321 ttttagttcc aatatcttcg acggtttttc ttataccggc gcggcactgg caagtgacga
     1381 tcgaatgttg ccatgggaac tacgcggcta cgccccacaa attagcggta ttgcacagac
     1441 caatgccacg gtgacgatca gtcaatcagg ccgcgtcatt taccagaaaa aagtcccacc
     1501 aggcccattt atcattgacg accttaatca gtctgttcag ggcacactgg atgtcaaagt
     1561 gacggaagaa gatggtcggg tgaacaattt ccaggtttcg gcagcatcga cgcccttcct
     1621 gactcgtcag ggacaggttc gctataaact ggccgcgggt cagccacggc cctccatgtc
     1681 acatcaaact gaaaatgaaa ccttttttag caatgaagtt tcctggggga tgctgtcaaa
     1741 cacctcgctg tacggcggcc tgctgctttc tggtgatgac taccattctg ccgcaatggg
     1801 tattgggcaa aatatgctgt ggcttggtgc gctgtcgttt gatgtcacgt gggacagtag
     1861 ccattttgat actcagcagg acgagcgggg cttaagctac cgttttaatt acagcaaaca
     1921 agtggatgcc actaacagca cgatttccct cgccgcttat cgtttctccg atcgtcattt
     1981 tcacagctac gccaactatc tggatcacaa atacaacgac agcgatgcgc aggacgaaaa
     2041 acagacgatc agcttatctg tgggccaacc gattacccca ctaaacctca atctttacgc
     2101 caacctgcta catcaaacct ggtggaatgc agacgcctcc acgaccgcca acatcacggc
     2161 aggttttaat gttgatattg gtgactggag agatatctct atttcgacgt cattcaatac
     2221 aacccattac gaagataaag atcgcgacaa ccagatttac ctgtcgattt cgctcccctt
     2281 cggtaacggt ggtcgggttg gctatgacat gcaaaacagt agccacagca ccacacaccg
     2341 catgtcgtgg aacgatacgc tggatgaacg taatagctgg ggcatgtctg ccggactgca
     2401 atccgaccgt cctgacaatg gagcccaggt gagcggtaac tatcagcacc tgagttcagc
     2461 gggtgagtgg gatatttctg gtacctatgc cgccaatgat tacagttccg tcagcagcag
     2521 ctggagcggt tctttcaccg caacccaata tggtgcagcg tttcatcgcc gcagctccac
     2581 caatgaacca cgcctgatgg tcagcaccga tggcgtggca gatattccgg ttcagggcaa
     2641 tctcgactac accaaccatt ttggcattgc ggtggtgccg ttgatttcca gttaccagcc
     2701 ttccaccgtg gcggtgaaca tgaatgactt acccgacggc gtaacagttg cagaaaacgt
     2761 tatcaaagaa acgtggattg aaggcgcgat aggttacaaa tcactggctt cccgttccgg
     2821 taaagacgtt aacgtcatca ttcgcaacgc cagcggtcag tttcctcccc tcggagcgga
     2881 tatccgccag gatgacagcg gcattagcgt ggggatggtt ggcgaggaag gacatgcctg
     2941 gttaagcgga gtcgctgaaa atcaaaagtt taccgtggtc tggggtgata gccagcattg
     3001 ctcgctccat cttcctgaac atatggaaga caccgcaaat cgcctgattt taccttgtca
     3061 ttaattaagg aaatgaaaat gacatttatg aaaggactac cccttttgtt gttggttgcc
     3121 agtctgtgca gccacgctgc actacaaccc gatcgcactc gtattgtgtt taacgctaat
     3181 gataaagcta ccagtctgcg ggtggataat cgtagcgata aactccccta cctcgcttat
     3241 tcctggctcg aaaatgaaaa aggggagaaa agcgatgatc ttctggtcgc cctgccgccg
     3301 atacaacgtc tggaaccgaa agcaacaacg caggtacgga tagtaaagca agcctcgacc
     3361 acaaaattac cgggtgatcg cgaaacgcta tttttctaca atatgcgtga aattcctccc
     3421 gccccggaaa aaaacagcga ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag
     3481 gtgttctggc gaccgactgc attacgcaag aaagcaggag aaaaggtcga actgcagtta
     3541 caggtcagcc agcagggcaa ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc
     3601 attgcctacc tgggacgaaa tgaaaaaggc gttctccccg gcttcaaaac cgtgatggtt
     3661 gcgcctttta gtacggtcaa cacaaacacc ggaaattata gcggcagcca gttttatctc
     3721 ggttatatgg atgattacgg cgcgttacgc atgacgacgc tcagctgcag cggacaatgc
     3781 catttacaag ccgtggaggc gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct
     3841 gtctgttatt tctgccgttg aagtctgcgc ttgccctgaa ctgctacttt ggttcatccg
     3901 gtggctcggt agaaaaatca gaagcgattc aaccttttgc tgtaccaggc aatgccaaac
     3961 tcggcgataa gatttgggaa tctgacgata ttaaaattcc cgtctattgt gacaacaata
     4021 ccaacggcaa ttttgaaagt gagcatgttt atgcctgggt aaacccctac ccgggggtgc
     4081 aggatcgcta ttatcaactc ggcgtaactt acaatggcgt tgattatgac gccaaccagg
     4141 gaaaaagccg catcgacact aaccagtgta ttgacagtaa gaacattgac atttataccc
     4201 ctgagcagat tatcgcgatg ggatggcaga acaaaatttg ctcgggtgat cccgctaata
     4261 ttcacatgtc gcgtaccttt ctcgctcgca tgcggttata cgtcaaaata cgagaaatgc
     4321 cgccgcatga ttatcaaagt acgcttagcg actatatcgt cgtgcaattt gacggtgccg
     4381 gtagcgttaa cgaagaccct actgcccaaa acctgaaata tcatattact ggtctggaaa
     4441 acattcgcgt gctggattgc agcgtcaatt tttccatttc accggaaaca caggtgattg
     4501 attttggtaa atttaatttg ctggatattc gtcgccacac aatgtcgaaa acattcagta
     4561 ttaagacgac aaaatcacaa aatgaccaat gcaccgacgg atttaaagtc agttcctcct
     4621 tttataccga agaaacgttg gttgaagaag ataaagcatt gttgattggt aatggtctga
     4681 agctacgttt actggatgaa aacgcctcgc cttatacctt caataaatat gctgaatacg
     4741 ccgatttcac cagcgatatg ctgatctacg aaaaaaccta tacggctgaa ctttcgtcca
     4801 ttgcaggcac ccccatcgag gctggcccct tcgatacagt ggtgcttttt aagattaact
     4861 ataactgatt tgccatgatg ccctttgcag ccggaaaggg catctctttc agaaaattgc
     4921 tgattttccc cttttccttg attgctgtta ttttttcctg gcccggcttt ctttatgctg
     4981 tatcgataca ccaaagagaa aataatgagg gagcgaagga tgccagtttt gcagtgggga
     5041 atgttatgtg tgctatcact tctcctttct attggtttcc tcgcggtaca tctcccggcg
     5101 gcgctattac ttgggccgat gatcgccggg atcatcttca gtatgcgcgg aattactctg
     5161 caacttcccc gctccgcttt tctcgccgcg caggccattc ttggctgcat gattgcgcaa
     5221 aacctcactg gttcgattct caccacgctg gccgccaact ggccgatcgt gcttgctatt
     5281 ttactggtga cgctgctttc cagcgccatc gtgggttggt tattggtgcg ctatagctca
     5341 ttgcctggaa ataccggtgc ctggggttct tctcctggcg gcgcggcggc aatggtcgct
     5401 atggcgcaag attacggcgc agatattcgc ctggttgcat ttatgcaata tctgcgagtg
     5461 ctgttcgtcg cgggggccgc tgttttggtc acccgcatga tgctgggtga taacgctgaa
     5521 gcggttaacc agcaaatcgt ctggttcccg ccagtgagca ttaatctcct gcttacgatt
     5581 ttgctcgcgg tcgttgccgg tacggcggga tgcttgctac gttttccttc cggcacgatg
     5641 ctcatcccga tgctggcggg cgcagtgctc cagtctggtc agctcatcac catcgaactc
     5701 ccggaatggc tgcttgcgat ggcgtatatg gcaattggct ggcggattgg tcttggtttc
     5761 gataaacaaa tcttactgcg ggcactacgc ccgctaccgc aaatcctgct gtcgattttt
     5821 gctctgttgg ctatttgtgc gggtatggcg tgggggctga cccggtttat gcatattgat
     5881 tttatgactg cctacctcgc caccagcccc ggcgggcttg ataccgtggc ggtcatcgcc
     5941 gcagggagca atgccgatat ggcgcttatt atggcgatgc aaaccctgcg cctgttcagt
     6001 attttgctga cgggacctgc cattgcacgg tttatttcaa cctatgcgcc gaagcggtcg
     6061 gcttagtgct ggcagccagg gcaccagtaa aacgggcgag atgacagcgt ggttttctca
     6121 atgatgctgc cacaacgttc gcacggttcg ccatctcgat gaaaaacctt aaagcgaaac
     6181 agcgccccat gatgcttatt ctcatccacc tgcccccgcg tagcgtaaga aaatcgagga
     6241 atctccagta acgcgtgtgc gagtgcatcc agttgcgccg cattgagatc tttcgcttta
     6301 tgatttccag tcaacccaac ctgccagagg atctccaccc gcaaataatt gccaagtcct
     6361 gccagaaacg cctgctcgag cagtaatcct gcaaactgac ggttacgaaa gcgcggcgac
     6421 aatacccgtt ctttcaccac ctccggcgtc agattcggat ccagcacatc gggaccaacg
     6481 cgttgtaaaa acggatgcgt ggtcagttgt tccggggtca acatctcaat atccgaggcg
     6541 ctataaagca gaatggtttt gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc
     6601 tgcggctctt cgccggtatc aaccacgcgc cagacgccgt aaagctgatt atggctgtag
     6661 agcgttaatt cgttggaaaa atgagttaac aacgccttac cacgcgtttc cacatgggta
     6721 acgtgttgac cgataagttg tgattgataa gtttttaact gcgggaaggc aaaccagaca
     6781 tcagttagtg gtttgccttt gatcgccgcc tccaggttat ccgctgcacg gcggatctcc
     6841 gggccttcag gcatggtgat atcctttttt cattctttat taagcggggt tatgctgcga
     6901 caacaatccc ctttttggca aatgtagctc gtaatcggcg ggcgaacgcc agagcgtgct
     6961 cgccgtcgcc atgcaggcag accgtttgcg ccgcgaccgt tgcccattcg ccggtgatac
     7021 ttttgactct gccgtgttgc accatctcca gcgtttgcgc cagcgcctgt tgttcgtttt
     7081 caatcaacgc gcctggctga cttcgcggca ccagcgagcc gtcagcctga taaccgcgat
     7141 cggcaaacac ttcctcgcgc gttgtcaggc catattgctt gcctgcacga atcagctcgc
     7201 ttcctgccag cccaacgaga atcaatgccg gatcgcaagc gtataccgct ctggcgatgg
     7261 cgtctgccag ttgtgcttct ttcgccgcct ggttgtacaa catgccgtgc ggtttgacat
     7321 gacgcattac gccgccttgc gcacgggcaa tcgttgccag cgcgccaatt tgatacagcg
     7381 tctgggcgta aacggtttct ggcggcagct gcatgccgct gcgaccaaaa ttttccctgt
     7441 cgggaaaact cgggtgagcg ccaattgcaa caccattttt tattgcttca cgcacgcaag
     7501 catgcatggt ttgcgcatcg cctgcatgaa atccacaggc aatattggca gaggaaacca
     7561 gcgttaatag ctctgcgtcg ctggcgcagc cttcgcccag atcggcgttc aggtcaattt
     7621 tcattgtgca gccgccacgc taattgttca aaataacgtt gctgatcttg ccgcgctttc
     7681 agtgcctctt ccagtgaaca ctggacaaaa tgaatcggct gaccgagcgg aatttgcgca
     7741 agatggtaca tatcagcctc aatgatgcag gcaatacgcg ggtaaccacc ggtggtctgt
     7801 gcgtcgttca tcaacacaat cggctgcccg ttatgtggca cctgcaccac gcccggtaac
     7861 aaaccgtgag ataacagttc gcgatcggtg gtgcgtttta aaatttgccc ctgtaagcga
     7921 tagcccatgc ggttactttg cgggctaagc tgccagggcg aacgccagaa tgcatcctgc
     7981 gaggcgcgat cgaactcatg atattccggc cccggcaagg cgcgaatgcg gttgccccac
     8041 agcagctgtt taacgccctg cgcttccata aaatcacgct tcgatttgcc aatcgggagt
     8101 cggtcaccat ccttcagtaa acggccttcc agcccgccaa tccccacgtt gagatcggtg
     8161 ctgcatgacc ccattaccgg cggaacatca ataccacccg cgaccgccag ataactgcgc
     8221 atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg ctttcatcgg caatcgccag
     8281 ccggtccaga cggcattatc atccagccgc gcttcgcaac cggcacccgt cagagcaaac
     8341 cacccatcag tttcgaactc aacagttaac tgaccgagcg tgatctccag cgcgggggca
     8401 ttggcgtcat tacccaccag taggttagca atgcgtaacg cgggcatatc cagtgcgccg
     8461 cagtggctga tacccgactg gcgaaaaccg tgacgaccgc catcctgcac agtggtatac
     8521 atgcccgcac gaataatctt cagcatactc cctccttctg cggtacaaag cgcacgctgt
     8581 ctcccggacg taataagatg ggttcgtcac gcgccggatc aaacaggctg agtgaggtat
     8641 gaccaatcaa ctgccagcca cccggcgttg ccagcggata aacaccagtc tgcggcccgc
     8701 cgatcccgac agaacctgcc ggaacgagta agcgcggttc agcgcgccgt ggcgtgtgta
     8761 gttgttccgg caaactcccg agatacggga agcccggttg aaaacctaaa aaccagacca
     8821 cgtattccac ggaggagtgc aattcaacaa cctgtttttc gctcaacccg caatgcgccg
     8881 cgaccaccgc caaatccggt ccgcctgcac caccgtaaac caccggaatt tcaataaagc
     8941 gagactcagg ttccagggcc tcgctctcct cccaccagcg ttgcaaacgc tctatggcat
     9001 ccagcgccag cgactcagga ttacgcaaaa tcaccgtaat attgttcatg ccgggaatgg
     9061 cttcaaccac attcggcata tccaccagac gctgcgccag tcgccagatc cgtttctggc
     9121 tagccagcgt caccggcggt tccagttcca gcactaccgc cgtttcacct atcagataac
     9181 aacgcgctcg ttgcactttc gatccctctg attattcgtt atgcaggatt aggaatatca
     9241 ataaaggtca catcaagatc ggtattttca ttcagccact cgctcaatgc gcgaatacca
     9301 ccacgttcag tggcatggtg acctgcagca taaaaatgca atccctgctc gcgggctgaa
     9361 tgaatggtct gttcagaaac ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg
     9421 ctatcgataa aactttgccc gccgcccgtg caccaggcga cgcgctggac cacctcaggt
     9481 ccggtatcgc cacaccataa cggcttacgt cccagacgcg cttcaatcca ggaggctagt
     9541 tccagtcccg gcacaggcat ggtcagctct ccccacggca ccaacggctc aatttcgccc
     9601 atgaccgtga tccccagtaa cgccgccagc tgtgcgttat tgcccagctc aggatgtgcg
     9661 tcaagcggca aatgccagcc atacaggttg atatcattcg ccagcaacgt ttttaaacgg
     9721 ttacgcttca ttccgcgaat gaccggagac tctcctttcc agaagtagcc atgatgcaca
     9781 atgaccgcat ccgcgcccag acgcactgcc tcatcgagca aagcctggct ggcggtgaca
     9841 ccggtaacaa ttttttgcac cgtctctttg ccttccacct gcaaaccatt cggcgcgtaa
     9901 tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt gttccagttc ggtgtttttc
     9961 atcttcgcgc cttccgtatt ctgatagcta caccgttaac atagcgttac ctcgccagtt
    10021 cgtcgatagc caatcttttg acatattaag aaacaaaccc gacgccaaaa gacgcaaatt
    10081 gtttatatca tcacgcttca gctaatgtat atttatgcaa tcagacagca tattgcataa
    10141 ataatcagat ctgtatcaaa gttttaactt taaggaaaca caagcacgcc gcgcaactat
    10201 tacgtgacgt tgccgtcaat tgtggaatta ttatgaataa acacgcatca cagccgcgcg
    10261 ctatttacta tgtcgttgcg ctgcaaatct gggaatattt tagcttttac ggcatgcgtg
    10321 ccctgctgat tctctatctc accaatcaac taaaatacaa cgatactcac gcctacgagt
    10381 tatttagcgc ctactgttcg ctggtgtatg tcacgccaat cctcggtggc tttttggcgg
    10441 ataaagttct cggcaatcgc atggcggtga tgctgggggc gttgttgatg gcgatcggtc
    10501 atgtggtgct gggtgccagt gagatccatc cgtcattcct ctatctgtcc ctggcgatta
    10561 tcgtctgcgg ctatggcctg tttaaatcta acgtaagctg tctgctcggc gagctgtatg
    10621 agccaaccga tccgcgtcgt gatggcggtt tctcgctgat gtatgcggcg ggtaacgtgg
    10681 ggtctattat cgcacctatc gcctgtggtt acgcccagga agagtacagc tgggcgatgg
    10741 gctttggcct ggcggcggtt ggcatgatcg cgggtctggt cattttctta tgtggcaatc
    10801 gtcatttcac tcatacccgc ggcgttaaca aaaaagtact gcgtgcgaca aactttctcc
    10861 tgccgaactg gggatggctg ctggttctgc tggtggcaac gcctgcactg attaccgtac
    10921 tgttctggaa agagtggtcg gtatacgcct taattgtcgc gactattatt ggcctgggtg
    10981 tactggcaaa aatttatcgc aaagcagaaa accagaaaca gcggaaggag ctggggctga
    11041 ttgtgacgct caccttcttc agtatgttgt tctgggcctt cgcacaacag ggcggtagct
    11101 cgattagcct ttatatcgac cgcttcgtta accgcgatat gtttggttat accgttccga
    11161 ccgcgatgtt ccagtcgatt aatgccttcg cagttatgct gtgcggtgtg ttcctggcgt
    11221 gggtggtaaa agagagtgtc gcgggtaatc gtaccgtgcg catctggggg aaatttgctc
    11281 ttggccttgg cctgatgagc gccggattct gcattctgac cttaagcgcc cgctggtccg
    11341 caatgtatgg tcactcttct ctgccactga tggtattagg cctggcggtg atgggctttg
    11401 cggaactgtt tatcgacccg gttgccatgt cgcaaattac gcgcattgaa atccccggtg
    11461 tgaccggcgt attaaccggc atctacatgc tgctttctgg cgcgatcgcg aactatctgg
    11521 cgggcgtgat tgccgatcag acatcgcagg cttcgtttga tgcttccggg gcgatcaact
    11581 actccatcaa tgcatatatt gaagtatttg atcaaattac ctggggcgca ctggcgtgtg
    11641 taggactggt actgatgatt tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg
    11701 cgctggagtc ataataggcc agcaccatac ctgataaaac gctcgctgcg ttttatcagg
    11761 ctcctttcct cgcttcttcg tacgctgcca gcgttcgcag ccttgcctct ttgtgatcaa
    11821 ctatcggttg cggataatcc agcatcacac ctgctttctg cgcccacttc cacggctcat
    11881 gcaccgcttt ccctggtaca tcgcgcagct ccggtaacca tcggcggata aactcacctt
    11941 cgcgatcaaa tttctcgccc tgggttgtcg ggttgaaaat acgaaaatac ggcgctgcat
    12001 cagtacccgt agacgccgcc cactgccagc caccgttatt ggctgccaaa tcaccatcaa
    12061 tcagctgcga catgaaatat cgctcgcctt cgcgccagtc gatcaacaaa tctttcacca
    12121 gaaaactggc tgaaatcatc cgtagccggt tatgcatcca gccagtgctg ttaagctgac
    12181 gcatggcagc atcgacaatc gggtatcccg ttttgccttc ctgccaggcc tgtaaatgtg
    12241 cgggattgct ctgccactgc acacgatccg tccaggcaat aaacggacaa tgtttacaca
    12301 acgaggggta atacgtcatc agatgacggt agaactcgcg ccagatcagc tcactaagcc
    12361 agacactacc ggccccaccg tccagcgcct gcggctgttc cgccaacaag cgatgcaaac
    12421 actggcgagg cgataacccg cccgttgcca ggctggcgga caaacgactg gtgccttcca
    12481 ctgccggaaa atctcgttgt tgctcatatt ctccggcacc gttctggcaa aactggcgta
    12541 attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa gactgacgag
    12601 gataattcag cgtaatggat ggcgcgggct ctatcgatcc gctactacga acttttggcg
    12661 cagcgacaca ctccggcatc ccttcccgca gccgtttcag ccaggcattc ttaaaaggcg
    12721 taaagacttt gtacatctcg tgattaccgg tcatcaccgc gccaggcggc aggatcacgc
    12781 tgtcatcaaa tccttcacac accacgttac gcagcgctct ttcaacttcc acatcccgcg
    12841 cccgctcatt cacttcatac tgatagttat aaaacaggtg ggtaacgctg ttttccgcgc
    12901 acacctgttt aactatttcg acactggcga caaagtcatc cacttcacgg aacaataaag
    12961 gaataccttt ttccgcaagc gctatttgta gcccattcag ttgagcattg ataagttcag
    13021 cctgacgcgg cgacatgtta tgcgtcgccc actggcgtgg tgtagcgata tataacgcca
    13081 gcacgcgtgc agacgaattg cggcaggcgg cagccagtgc gagattatcg tgcagacgta
    13141 aatcctggcg aaaccagacc agatgggtaa tcataaaact cctgaatggc gcaagttaat
    13201 cacacgcggc caaagttcga aataacgctg ccctgaaagc caggcgtcag gataaagcgc
    13261 catatagtgt ttgatacgca tcagcggcgc aagaagtggt tgctcgccac ggcgataact
    13321 gtcgataagc gccgccaact gctggcgttc tgtggaatca atatgcgggc gaaaataacc
    13381 ctgaacgtgc atcaaaacgt tggtgtgatc gcgcgggttg gcgggatgag aaagcaaaac
    13441 aattacgcgt tggtagtact ggttgtaaaa atcatccaga ttttgccact ggtgaatatc
    13501 agccacgaac gggccaagtt tgcggtactc cggctgagaa tgcgccagaa agacgagttt
    13561 atagcggctg tgataatcga gaagcgcccc gcgcgttaag ccatgttctt tcagtcgatg
    13621 taactcatga agggcgaaga tacggattag ggtgctgtca tcaaatcgtt gctgattcat
    13681 tactgctcct gaacggttta caggagtaag tgtagacgtt gagaaatgat atttgtgaag
    13741 ttaatcctgt tgtagcagga caaatttacg aaagcctgct ccccgcaagg actgacgtca
    13801 ggtagtttct aaccatagct gccctttcta ttcaaac
//
LOCUS       DAJSKJ010000107        13771 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000107 DAJSKJ010000000
VERSION     DAJSKJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.107"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            54..707
                     /locus_tag="ON345_002625"
                     /pseudo
     CDS             54..707
                     /locus_tag="ON345_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1705 domain-containing protein"
     gene            complement(643..1670)
                     /locus_tag="ON345_002626"
                     /pseudo
     CDS             complement(643..1670)
                     /locus_tag="ON345_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1868..1943)
                     /locus_tag="ON345_002627"
     tRNA            complement(1868..1943)
                     /locus_tag="ON345_002627"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:Phe,seq:gaa)
     gene            complement(2049..2756)
                     /locus_tag="ON345_002628"
     CDS             complement(2049..2756)
                     /locus_tag="ON345_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR3072666.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3154..5289
                     /gene="speC"
                     /locus_tag="ON345_002629"
     CDS             3154..5289
                     /gene="speC"
                     /locus_tag="ON345_002629"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR3072667.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEIHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGVKRLYGYVLK"
     gene            complement(5339..6595)
                     /gene="nupG"
                     /locus_tag="ON345_002630"
     CDS             complement(5339..6595)
                     /gene="nupG"
                     /locus_tag="ON345_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR3072668.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6797..7876)
                     /gene="mltC"
                     /locus_tag="ON345_002631"
     CDS             complement(6797..7876)
                     /gene="mltC"
                     /locus_tag="ON345_002631"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR3072669.1"
                     /translation="MKKYLALALIAPLLISCSTTQKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7941..8216)
                     /locus_tag="ON345_002632"
     CDS             complement(7941..8216)
                     /locus_tag="ON345_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR3072670.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8244..9296)
                     /gene="mutY"
                     /locus_tag="ON345_002633"
     CDS             complement(8244..9296)
                     /gene="mutY"
                     /locus_tag="ON345_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR3072671.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9457..10176
                     /gene="trmB"
                     /locus_tag="ON345_002634"
     CDS             9457..10176
                     /gene="trmB"
                     /locus_tag="ON345_002634"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR3072672.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10176..10502
                     /locus_tag="ON345_002635"
     CDS             10176..10502
                     /locus_tag="ON345_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR3072673.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10686..11405
                     /locus_tag="ON345_002636"
     CDS             10686..11405
                     /locus_tag="ON345_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR3072674.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11581..12627
                     /gene="ansB"
                     /locus_tag="ON345_002637"
     CDS             11581..12627
                     /gene="ansB"
                     /locus_tag="ON345_002637"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR3072675.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12744..13751
                     /locus_tag="ON345_002638"
     CDS             12744..13751
                     /locus_tag="ON345_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR3072676.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
BASE COUNT         3476 a         3293 c         3616 g         3386 t
ORIGIN      
        1 cagggaattt ctggttctta ttatcttcat aataatttta tcgatatatg ccgatgaata
       61 tcagaaaact gttttgtccg ggcaatacgt cccggatttt attgttttta ttctttttcg
      121 ttatttccgt aataatcaca attgcatgcg gatacactga gaagaatgcc acagggaatg
      181 tattgcttct gtttctcctt cttctccttg cacacagaaa taccctcaca tccattacag
      241 cgctgttatt tctgttctgt tgtacactgt atgcgcctgc cggtatgacg tacggtaaaa
      301 tcaacaacag ttttattgtc gcgttgctgc agaccacggc tgatgaggct gcagagttta
      361 ccgggatgat tcctgtttat cattttctgg tcagtgctgc ggttctggta ttcatggtga
      421 ttttctggcg tacacaccac cgcggtcacc gtaactggct ggcactgctg ctgttcgtat
      481 tatgctctgt aaacagctgg ccgttgcgga tggttaaagg aaccgttgtg ggaacaactg
      541 actcattgcg tgaaatgcag cgttataaac aactgagtca gcacggggct gacagctgga
      601 aaatcctgcc gggtgtaccg ttgtatgaca cgattgttat tgtcactggt gagagtgtgc
      661 gcagggatta tatgtcggta tatggttacc cctcagtgcc ttatttacgg tgttttcact
      721 gattggttta cgcgggtcat gtgctcccgt aaatatcaga ccattttcac ctatgtctga
      781 agttctgtca gtatggctac agcctgtttg ctgagtggca caagatgtgg tgttcgcatc
      841 ttggagcccc ggtgtgagaa ttttacgcca ggcagaggtt cgcgttcggc tggaatagtc
      901 cacatggcat tgtcaatatc aatttctgac cagcgggcat atctgagttc actggagcga
      961 ataaacacca gcagattcaa cataacggca agccgggtga cagactgccc cttataactg
     1021 ccaatacgcg acagaagttc aggcaggcgt gaaaggtcaa gagcggggcg atgctggcgt
     1081 tttaccgtgg tcaatgcgcc agccatatcc agagcaggat tatatctgat gatgccagac
     1141 tgtacggcat aacgcattac agcggatatc ctttgctgca gacgactggc gatttcataa
     1201 atttgtttgg cttctgcggc gcgaacaggg ataagcaggt ccggggtact gagtgttgtg
     1261 atatcccggt tgccaataaa ggggaaaacg tgtgtctcga ggcttttgag tactttatcg
     1321 ctgtgtgatt gtgcccattg tttattactg gcatgccagc gtcgtgcaat atgttcaaaa
     1381 gagagttgta aatcagggac tactttttta gctcttttct gttcgcaagg gtcaatcccc
     1441 tccgaaatca gttttcgggc ctcatcccgt tttgtacgag cttcggagag agaaacttcc
     1501 ggatacacac caagtgccag agtcttctcc ttacccagaa tacgataacg gagacgccag
     1561 taacgggaac cattgggatg taccagcagg aacatgccag ccccgtcagt gagtttataa
     1621 gccttgtcag tgggctttgc agcccggatt tttgcgtcag tcagtgccat agtacagcct
     1681 tctgaatgcg ttaaagaggg tacaaattct gtcgaaccgg ggtatacccg caattatacc
     1741 cacacaatga ggttgatgtg gggggaatct ggttgagttc ggtagaattg atttggaggc
     1801 agaacgctta aattgtggcg ttctgaaacg aaaaacggac ctccgtggag gtccgttgat
     1861 atgaatttgg tgcccggact cggaatcgaa ccaaggacac ggggattttc aatcccctgc
     1921 tctaccgact gagctatccg ggcaacgggg cgcattaaac ctgattcgcc tcgtctcgtc
     1981 aatcaaattt cttcaattta ctgcagactg cacaatctat catcactttg ccgctattgc
     2041 acgcattgtc aggcaaacca ggcggtccag gcggcggaaa gcggcattgc cagaaggagt
     2101 gctggaagca tgttgaccac cgggaacatt ttaatgccac agatgcgtaa tccggttgcc
     2161 agcagcaata aaccgcctac ggcgctgaag tctgccatca tcgacggtgt ggtcagcggt
     2221 aatatcagcg cggcagccca cgccagcgtt aactggatga tcagtaatgg gatactaatt
     2281 accgacaccg caatacccag cgagcaggcg aagatcatcg ccgtaaagaa atcaagaaat
     2341 gacttggcga ttaaaatact cggatcgccg gtcatccctt cgttcatcgc cccgaagatc
     2401 ccggtgccgc tggcgcaaaa caggacaata atcgcgacat aattctgaat aaaagattca
     2461 tgcgctggct tcttacgtga gtgacgaaac agattttgtg ctttggcgac tgctgtattg
     2521 acacctttct ccagcaaaca aatttcgcca attagagcgc caagtagcgt agctaaaacc
     2581 atcgctggaa ggttggcgca tttcacaacc agtaaaatac caatccccag cgatgccaga
     2641 ccaaaaatag atgtcatgga gacgcggata cgttccggta agcgttggct gagcagtgcg
     2701 ccgagaacgc cacccagtaa gactgcacta gcgttgataa aagggccgat gaccacaaga
     2761 gttcctgtta gctgtattgt ttgattttct tattatgaac tttttgcctt gttgatggct
     2821 tgtctggtcg caaagtcctg gcttgcacgc tttagcgaaa ggtgccatga ttgcgcgaat
     2881 tttctcctct ctgtacggag tttgcccgat gcacgccatc tccttacatt ctctcgctta
     2941 tcgccgtttc gcgcgaaacg tttccctttc tatgttactg ctcatgcggt gaagttaacg
     3001 cacgctcact gcaggacaac agtaaaatca gagcgtttct gcttttactg atgtctggcg
     3061 gtcggagctg gtgaccagtt tgacccatat ctcatggggc agggttttcc accttgtccg
     3121 gtattcttac ttccccgaaa cgggtttgcg cttatgaaat caatgaatat tgccgccagt
     3181 agtgaactgg tatcccgact ttcttctcat cgtcgcgtgg tggcgttggg agatactgat
     3241 tttacggacg tcgcggcagt cgtcattacc gctgcggata gtcgcagtgg cattcttgcg
     3301 ttgcttaagc gcaccggttt tcatctaccg gtgtttttgt attccgaaca tgctgttgaa
     3361 ttacctgcgg gcgttacggc ggtaatcaac ggcaacgagc agcagtggct ggagctggaa
     3421 tccgcagcct gtcagtatga agagaatttg ctgccaccgt tttatgacac gctgacgcag
     3481 tacgttgaga tgggcaacag cacctttgct tgccctggac atcaacatgg tgcgtttttt
     3541 aaaaagcatc ctgccggacg ccatttttac gatttctttg gtgagaacgt ctttcgcgcc
     3601 gatatgtgta acgctgacgt aaaattgggc gatctgctta ttcatgaagg atcggcgaaa
     3661 gatgcgcaga aattcgcagc caaagtcttt catgccgata aaacctattt tgtgctgaac
     3721 ggcacatcgg cagcgaataa agtggtgacg aatgcgctgt taacgcgtgg cgatctggtg
     3781 ctcttcgacc gtaacaacca taagtcgaat catcacggcg cgctgattca ggcgggggcg
     3841 acgccggtct atctggaagc ttcacgcaac ccgtttggtt tcattggcgg tattgatgcg
     3901 cactgtttta atgaagagta tctgcgccag caaattcgcg acgttgcgcc agaaaaagcc
     3961 gagctgccgc gcccgtttcg cctggcgatt attcagctgg gaacctatga cggaactgtc
     4021 tataacgccc gtcaggtgat cgataccgtt gggcatctgt gtgattacat tctgtttgat
     4081 tccgcgtggg tcggttacga acagtttatc ccgatgatgg cggatagctc gccgctgcta
     4141 ttagaactta acgaaaacga tccggggatc tttgtgactc agtcggtgca caaacagcag
     4201 gcgggattct cacagacgtc gcagatccat aaaaaagata accatatccg cggacaggcg
     4261 cgtttttgcc cgcataagcg gttgaataac gcctttatgc tccatgcttc taccagccct
     4321 ttctatccgc tgtttgctgc actggatgtt aacgccaaaa ttcatgaagg ggagagtggg
     4381 cgtcggctgt gggctgagtg cgttgagttg ggtattgagt cgcgcaaggc tattcttgcg
     4441 cgctgtaagc tgttccgccc gtttatcccg cccgttgttg atggcaaatt gtggcaggat
     4501 tatccgacat cagtgttagc cagcgaccgc cgttttttca gttttgagcc gggggcgaag
     4561 tggcacggct ttgaaggata tgccgcggat cagtattttg ttgatccgtg caagctgtta
     4621 ctcactacac caggtatcaa tgccgaaacc ggcgaatata gcgactttgg cgttccggcg
     4681 acgattctgg cgcactatct gcgtgagaac ggcattgtgc cggagaagtg cgatctcaac
     4741 tccattctgt tcttattaac tccggcggaa agccacgaga agctggcaca actggtagag
     4801 atgttggcgc agtttgaaca gcatattgag gatgactcgc cgctggctga ggtgttgccg
     4861 agcgtttata acaaatatcc ggtgcgctat cgcgactaca ctttgcgcca gttgtgtcag
     4921 gagatacacg atctgtatgt cagtttcgac gtcaaagacc tacaaaaagc gatgttccgc
     4981 cagcagagtt tcccgtcagt ggtgatgaac ccccaggatg cgcatagcgc ttatattcgc
     5041 ggtgaagtgg agttggtgcg gattcgtgat gccgaagggc gaattgcggc agaaggggcg
     5101 ttgccttatc cacctggcgt gctttgcgtg gtacccgggg aagtctgggg tggggcggtt
     5161 caacgttatt tccttgcact ggaagaaggg gtgaatttgt tgccgggatt ttcgccggag
     5221 ctgcaaggtg tttatagcga aaccgatgcg gatggcgtga aacggttgta cggttatgtg
     5281 ttgaagtaag aataaaaaaa acgggtcacc ttctggcgac ccgtttttct ttgcgtaatt
     5341 agtggctaac cgtctgtgtg cctgtcggga cacgaacgtg tttatatttg aacatcgcca
     5401 tgaacgcgaa ggccagaacc acggagtaac cagcgaaaat caaccatacg gtctgccagt
     5461 cggtaatgcc gttttgggtg tacatctcaa caactttacc gctcacgatg ccgccgagga
     5521 tacagccgaa gccgttagtc atcatcagga acatcccttg tgcactggcg cgaattgccg
     5581 ggctaacttc tttttcgaca aacaccgaac cagagatgtt gaagaagtcg aatgcgcaac
     5641 cgtaaacgat catcgacagt accagcagta cagtaccgaa cggagtcggg tcgccgtaag
     5701 caaacagcgc aaaacgcagg atccacgcca caatactgat catcattacg ttcttaatac
     5761 cgtagcggct taagaagaac gggatggtca gaatgaacag ggtttcagag atctgcgaaa
     5821 tcgacatgat gattgacgca tgctgcacaa taaagctgct ggcaaacatc ggatctttgt
     5881 cgaagctgtg caggaaggta ttaccgaaca tgttggtaat ctgcagttcc gcgcccagca
     5941 gcattgagaa gataaagaag attgccatac gcttgttttt aaacagcgcg aatgcatcga
     6001 ggcccagcag ggttgtccag ctctgattcg cttgctgttt agcaaccgga atatgcggca
     6061 gagtcagggt aaacagaacc agaatggcgg aaagtgctgc gccaatatac agctgcatgt
     6121 ggcttaattc gaagccagac aggctcacca cccacattgc catgataaag ccgatggtgc
     6181 cccagatacg gattggcggg aagtcagtaa cgatatccat cccggcattt tgcaggcgat
     6241 agtaagagat ggtgttgatt aacccaagcg ttggcatata agcaaacgag ttaatcaata
     6301 tcacaaggaa catcgcctcc ggtgtcgtga cctgtgccgc catgaacagc gtgatagcgc
     6361 caatggtgtg gcaaatggca tatacccatt tcgcacttaa ccatttgtcg gccacaatcc
     6421 ccagcagcgc aggcataaat accgcggcga tacccagtga gctataaact gcgccaatag
     6481 aagcaccgtc aaacttcagg gtaacaaaca tataggagcc gagggtcgtc agccaacttc
     6541 cccacagaca gaactgcaga aaagagagga ttttcagctg cagcttaaga ttcatgttaa
     6601 tttcctcaca tcgtgatgcg gatgagtgtt tgaaaaagca catttgaccg tgcgagatga
     6661 tgggattcta tcaacttcgc ggactggttt tttgttacct gtggcaaata attgcaaaca
     6721 ttcccaattt gcaaaacgaa attgtgatgt ggataacatt tttgcccctg agcatcgtca
     6781 ggggcggtta atggaattat cggcggcggt aggatttttg cgcggtattc actttataaa
     6841 gataacggcg agattccgca gagggatggc gggtcgtcag cgtctgataa acatcgcccg
     6901 gcgtcatggt gttaataata ttggcagcct gaatcttatc attcgaaaag actcgcagca
     6961 cgctgcctgc gccgccgtta taggcggtga tgacggcata acgccgcgat gttgggttat
     7021 caattccgcc gagataaaca ttgttcagca tcgccagata cgcggtgccg gtatcaatat
     7081 tgctggcagg atcaaacaag aaactgcggc tcggcgtgcc ggatttcccc tgcgagcgga
     7141 acacatcttt cccggcagta tgttgtacca cctgcattaa tcccagcgca tcggaacggc
     7201 tgaccgcata cgggttaaag gaagattcgg tctgcataat tgccagaatc agcgactcat
     7261 caacgccata tttccgtgac gcctggcgga ccatgccgag atatttgtgc gcacgtttat
     7321 caaggtggtt cggtaccatg ttaatggtga cgctgtagat gatacgcagc ccgttactgc
     7381 ggctttgcag acggtttttc agcagataat ccgcgaagtt gcttgcgcga ccttcccagc
     7441 gaatcggctg cccggtgttg tccaccacct gaccgtaaag gaaaggttct ttcgaaatcg
     7501 taatatcatc aacgtcggaa tagagatcga ccgaactcgg atcgtcaccc atcagcaacg
     7561 ttttgataat tgcccggcgc aaatgcgcgg caggttctgt cccggcgata gtttcgatag
     7621 taatcgtacc gtcatcgaag ttgatgtggc tgcgggtctg atattgatcg gtgtatttca
     7681 cgtagtcctt aggaccggcg atcaccacct ctttgaatcc ccagatgttc tcaatattgt
     7741 gggcaaattg ccccatcaga atatcaaaac cgttggtatc tttgacccag gcttcgttat
     7801 aggtatcgcc tttttgggtc gtcgaacagg agatgagcaa cggcgcaatc aaagccagcg
     7861 cgagatattt tttcatcatt ccgggtgcgt gttgtgtttg tttttatgca agttgccgga
     7921 ggcgtgctcc ggcactgttt ttatttttta tcttccggcg tatagccctc gatatgcacc
     7981 tctttaccct cgaacaggaa gttgaccatc tcctgctcaa gcagcttgcg gtgctcggca
     8041 ttcatcatgt tgagtttctt ttcattaatc agcatggttt gcttgtgctg ccactgcgcc
     8101 caggcttctt tggagatctc gttatagatg cgttttccca gctcgccggg gtacagctga
     8161 aaatcctgac cttctgcttc acgttgcagg aaagtacaaa aaatcgttct gctcataaat
     8221 catcctcttt atcgactcac gcgctaaacc ggcgcgccag tgcgtaactg ctgtaacaaa
     8281 cgctccacgg gagccgccag gccaactgac ggcggttgcg ctaagttata ccagagcgca
     8341 ttgccttcat ccatgcagcc ggtgaatgac gacacgggaa gccacatagg cacaatatct
     8401 aagtggaaat ggctgaaggt atgccgaaac gcggtcagtt gcgtcaggtt atcggcagca
     8461 atctgccgtt gcgccagcca ctgccgcaaa ctttcttcgt cggcaaactg cgggaaacag
     8521 tataaaccgc cccacaatcc gctcggcgga cgctgcgcca gcaatacttc atcttcgtgc
     8581 tgtaacagca aaaagtagcc ggtgcgctcc ggcagcgtct gtttcggttt tttgcccgga
     8641 taaagcgacc agctattatt ggcggcggca atacatccgt tttgtagcgg acagagcgaa
     8701 catttcggct tcgagcgcgt acaaatcatc gcgcccaaat ccatcatcgc ctgattaaac
     8761 cgttccacgc cgaccgcggg cgtcacctgc tcgctcaaac tccataattt attctcgacc
     8821 tcttttttcc caggccagcc gcttacagca tagcagcgcg ccagcacgcg tttgacgtta
     8881 ccgtcgagaa tcggaaagtg cttgcccaga gaaagcgaga gaatcgcgcc tgcggtggaa
     8941 cgcccgacgc ccggtaacgc cgcgacttct tcaaaggttt ccgggaattt accgccgtgt
     9001 aaggtcgcca cttgttgtgc cgctttatgc agattgcgcg cgcgggcgta atagccaagc
     9061 ccggtccaca agtggagaac ttcgtccagc ggcgcattgg cgagatcggt caccgtcggg
     9121 aagcgcgcca taaagcgttc aaaatagggg ataacggtcg caacctgagt ttgttgcaac
     9181 atcacttctg agagccatac tttgtagggc gtcttgtcaa tttgccaggg cagagttttt
     9241 cgcccgtatt tatcgtacca gtccagaacc tgggctgaaa attgcgacgc ttgcatggtc
     9301 accgaattca ctgttgttgg gggcaagatt gcagcacagc gacggcaggg tgtaaaccgg
     9361 aactttccgc agccacggcc ttatcatgct tgcatccggc aactaacttt ggataatgcc
     9421 cgttttcaga acactttcac aagcgactaa atctttatga aaaacgacgt catctcaccg
     9481 gaatttgatg aaaacggccg cccactgcgc cgtatccgta gttttgtgcg ccgccagggg
     9541 cgactgacca aaggccagga acatgcgctg gaaaactact ggccggtgat gggcgttgag
     9601 ttcagcgaag atatgctgga tttccccgcg ctttttggcc gtgaagcgcc ggtgacgctt
     9661 gagattggtt ttggcatggg ggcgtcgctg gtggcaatgg ctaaagatcg ccccgagcag
     9721 gacttcctcg gcattgaagt gcattcaccg ggcgttggtg cgtgcctggc ttctgcgcat
     9781 gaagagggct taagcaacct gcgcgtgatg tgtcacgatg cggttgaagt gctgcataaa
     9841 atgattcctg acaattcatt gcgcatggtg cagctctttt tccctgaccc gtggcacaaa
     9901 gcgcgccata ataaacgccg tatcgttcag gtgccgtttg ccgaactggt aaaaagcaaa
     9961 ctgcagctgg ggggcgtatt ccatatggcg accgactggg aaccttatgc ggaacatatg
    10021 cttgaagtga tgtcttctat tgacggttat aaaaacctgt cagagagcaa tgattacgta
    10081 ccgcgtccgg catcacgtcc ggtgacgaaa tttgaacaac gtggtcatcg tcttggtcac
    10141 ggagtatggg acttaatgtt cgagagggtg aaataatggc aaagaaccgt agccgtcgtc
    10201 tgcgtaaaaa aatgcacatc gacgaattcc aggaattagg attttcggtg gcatggcgat
    10261 tcccggaagg tacatcggaa gaacagattg ataaaaccgt tgatgatttt attaacgagg
    10321 ttatcgaacc gaacaaactg gcctttgacg gcagcggtta tctggcctgg gaaggtctga
    10381 tctgcatgca ggaaatcggc aaatgcaccg aagaacatca ggcgattgtg cgtaagtggc
    10441 tggaagagcg caaactggat gaggtacgca ccagcgaact tttcgacgtt tggtgggact
    10501 aagaaagcat acgggcgatg acaaatgcaa aactgcctga tgcgctacgc ttatcaggcc
    10561 tggaaagatg cacgatcgag taggcgggat aaggtgttta cgccgcatcc ggcatggaaa
    10621 acgcgtactt tgttatcaat ctggggccag caaatgctgg cctgattttt tcttgaggga
    10681 agactatgat gcgcaaaatg ctgctggcgg cagcactttc agtgacggca atgaccgctc
    10741 acgccgacta ccagtgcagc gtcacgccgc gtgacgatgt gattgtcagc ccgcaaaccg
    10801 tgcaggtgaa gggcgaaaac ggcaatctgg tgatcacgcc agacggcaac gtgatgtata
    10861 acggtaagca atattccctg aatgccgccc agcgcgagca ggcgaaggat tatcaggctg
    10921 aactacgcag cacgctgccg tggattgatg aaggcgcgaa aagccgcgtc gaaaaagccc
    10981 gtattgcgct ggataaaatt atcgttcagg agatgggcga aagcagcaaa atgcgcagcc
    11041 gtctgaccaa acttgatgcg cagctgaaag agcagatgaa ccgcattatc gaaacgcgca
    11101 gcgatggcct gacgtttcac tataaagcca ttgatcaggt tcgcgccgaa ggccagcaat
    11161 tagtgaatca ggcaatgggt ggaattttac aggacagcat taatgaaatg ggcgcgaaag
    11221 cggtgctgaa aagcggcggt aacccattac agaatgtgct gggaagcctg ggggggctgc
    11281 aatcctcaat ccaaaccgag tggaaaaagc aggaaaaaga tttccagcag tttggcaaag
    11341 atgtttgtag ccgcgttgtg actctggaag atagccgcaa agccctggtc gggaatttaa
    11401 aataatcctc tattttaaga cggcataata cttttttatg ccgtttaatt cttcgttttg
    11461 ttacctgcct ctaactttgt agatctccaa aatatattca cgttgtaaat tgtttaacgt
    11521 caaatttccc atacagagct aagggataat gcgtagcgtt cacgtaactg gaggaatgaa
    11581 atggagtttt tcaaaaagac ggcacttgcc gcactggtta tgggttttag tggtgcagca
    11641 ttggcattac ccaatatcac cattttagca accggcggga ccattgccgg tggtggtgac
    11701 tccgcaacca aatctaacta cacagcgggt aaagttggcg tagaaaatct ggttaatgcg
    11761 gtgccgcaac taaaagacat tgcgaacgtt aaaggcgagc aggtagtgaa tatcggctcc
    11821 caggacatga acgataatgt ctggctgaca ctggcgaaaa aaattaacac cgactgcgat
    11881 aaaaccgacg gcttcgtcat tacccacggt accgacacga tggaagaaac cgcttacttc
    11941 ctcgacctga cggtgaaatg cgacaaaccg gtggtgatgg tcggcgcaat gcgcccgtcc
    12001 acgtccatga gcgcagacgg tccattcaac ctgtataacg cggtagtgac cgcagctgat
    12061 aaagcctccg ctaatcgtgg cgtgctggtg gtgatgaacg acaccgtact ggacggtcgc
    12121 gatgtcacca aaaccaacac caccgacgta gcgaccttca agtctgttaa ctacggtcct
    12181 ctgggataca ttcacaacgg taagattgac taccaacgta ccccggcacg taagcacacc
    12241 agcgatacgc cattcgatgt ctctaagctg aatgagctgc cgaaagtcgg catcgtttat
    12301 aactacgcta acgcatccga tcttccggct aaagcactgg tagatgcggg ctatgatggc
    12361 atcgttagcg ctggtgtggg taatggtaac ctgtataaat ccgtgttcga caccttggca
    12421 accgccgcga aaaacggcac tgcagtagtg cgttcttccc gcgtaccgac gggtgctacc
    12481 actcaggatg ctgaagtgga tgatgcgaaa tacggcttcg tcgcctctgg cacgctgaac
    12541 ccgcaaaaag cgcgcgtcct gctgcagctg gctctgacgc aaaccaaaga tccgcagcag
    12601 atccagcaga tcttcaatca gtactaatca cctcgccccg gtatcgtgcc ggggcttttt
    12661 cacttcagac ttacgtccat tgccaatttt cattacccta atgataatca ccggaataaa
    12721 ttattccgcg tgaggttttt cgggtgaaaa agcaatggat tgttggcacg gcactgctta
    12781 tgttgatgac tggtaatgcc tgggcagatg gcgaaccgcc aactgaaaat atcttaaaag
    12841 atcaattcaa aaagcagtat cacggcattc tcaagcttga tgctatcacc ttaaaaaatc
    12901 ttgatgctaa gggtaatcag gccacctggt cagcggaagg cgatgtctct tccagtgacg
    12961 atctctatac ctgggtcggt cagttggcag attacgaact gctcgaacag acctggacga
    13021 aagataaacc ggtaaaattc tcggcgatgt taaccagtaa aggaacgccc gcgtctggct
    13081 ggtcggtgaa cttttactct tttcaggcgg cagccagcga tcgtgggcgg gtggtcgacg
    13141 atatcaaaac gaataataaa tatctgatcg tgaatagcga agatttcaat tatcgcttta
    13201 gtcagcttga atctgcgttg aatacccaga agaactcgat tcctgcgctg gaaaaggaag
    13261 tgaaagcgct tgataagcaa atggtggcgg cgcagaaagc agcggatgca tattggggaa
    13321 aagatgcaaa cggtaagcaa atgacccgcg aagaagcctt taaaaaaatt caccaacagc
    13381 gtgatgagtt taataaacag aacgatagcg aggcgttcgc ggttaaatat gacaaagagg
    13441 tttatcaacc ggcgattgcg gcatgtcata aacagagtga agagtgttat gaagtgccga
    13501 ttcagcagaa acgagatttc gatatcaacg aacaacggcg acagactttt ctgcaatcac
    13561 aaaaactcag ccgtaaattg caggatgact gggtaacgct cgaaaaaggg caatatccgt
    13621 taacgatgaa agtttcagaa ataaacagta aaaaagtcgc gattttgatg aaaattgacg
    13681 atatcaacca agctaatgaa cgctggaaaa aagataccga acaactgcgg cgcaatgggg
    13741 tgattaagta actcatgtta agtgcctttg c
//
LOCUS       DAJSKJ010000108        13396 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000108 DAJSKJ010000000
VERSION     DAJSKJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.108"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            236..571
                     /locus_tag="ON345_002639"
     CDS             236..571
                     /locus_tag="ON345_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR3072677.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            729..1172
                     /gene="yjdN"
                     /locus_tag="ON345_002640"
     CDS             729..1172
                     /gene="yjdN"
                     /locus_tag="ON345_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR3072678.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1305..2093
                     /gene="phnC"
                     /locus_tag="ON345_002641"
     CDS             1305..2093
                     /gene="phnC"
                     /locus_tag="ON345_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR3072679.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2118..3134
                     /gene="phnD"
                     /locus_tag="ON345_002642"
     CDS             2118..3134
                     /gene="phnD"
                     /locus_tag="ON345_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3072680.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3240..4019
                     /gene="phnE"
                     /locus_tag="ON345_002643"
     CDS             3240..4019
                     /gene="phnE"
                     /locus_tag="ON345_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR3072681.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSISFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4040..4765
                     /gene="phnF"
                     /locus_tag="ON345_002644"
     CDS             4040..4765
                     /gene="phnF"
                     /locus_tag="ON345_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR3072682.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4766..5218
                     /gene="phnG"
                     /locus_tag="ON345_002645"
     CDS             4766..5218
                     /gene="phnG"
                     /locus_tag="ON345_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR3072683.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5215..5799
                     /gene="phnH"
                     /locus_tag="ON345_002646"
     CDS             5215..5799
                     /gene="phnH"
                     /locus_tag="ON345_002646"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR3072684.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDKAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5799..6863
                     /gene="phnI"
                     /locus_tag="ON345_002647"
     CDS             5799..6863
                     /gene="phnI"
                     /locus_tag="ON345_002647"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR3072685.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6856..7701
                     /gene="phnJ"
                     /locus_tag="ON345_002648"
     CDS             6856..7701
                     /gene="phnJ"
                     /locus_tag="ON345_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR3072686.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IAHFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7698..8456
                     /gene="phnK"
                     /locus_tag="ON345_002649"
     CDS             7698..8456
                     /gene="phnK"
                     /locus_tag="ON345_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR3072687.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8632..9312
                     /gene="phnL"
                     /locus_tag="ON345_002650"
     CDS             8632..9312
                     /gene="phnL"
                     /locus_tag="ON345_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR3072688.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9309..10445
                     /gene="phnM"
                     /locus_tag="ON345_002651"
     CDS             9309..10445
                     /gene="phnM"
                     /locus_tag="ON345_002651"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR3072689.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRKSIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRRHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10445..11002
                     /gene="phnN"
                     /locus_tag="ON345_002652"
     CDS             10445..11002
                     /gene="phnN"
                     /locus_tag="ON345_002652"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR3072690.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10989..11423
                     /gene="phnO"
                     /locus_tag="ON345_002653"
     CDS             10989..11423
                     /gene="phnO"
                     /locus_tag="ON345_002653"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR3072691.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11425..12183
                     /gene="phnP"
                     /locus_tag="ON345_002654"
     CDS             11425..12183
                     /gene="phnP"
                     /locus_tag="ON345_002654"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR3072692.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12330..12659)
                     /gene="yjdP"
                     /locus_tag="ON345_002655"
     CDS             complement(12330..12659)
                     /gene="yjdP"
                     /locus_tag="ON345_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR3072693.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12728..13177)
                     /gene="rpiB"
                     /locus_tag="ON345_002656"
     CDS             complement(12728..13177)
                     /gene="rpiB"
                     /locus_tag="ON345_002656"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HCR3072694.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         3001 a         4026 c         3706 g         2663 t
ORIGIN      
        1 taaaaatatc atgtgaataa aaaaaagaac aagtagagca ttaacattat cttaaataat
       61 aaatagaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa acaatcgtcg
      121 tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg cggccttttt
      181 cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa tccacatgtc
      241 attaccacac tgcccaaaat gcaactccga atacacttac gaagataacg gcatgtacat
      301 ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa gcgacgagct
      361 gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta ccatcattaa
      421 agatctgaag gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag tgaaaaacat
      481 ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc gacggttttg gtccgatgaa
      541 actgaaatct gagtttgtga aaaagaactg attgtattgt gatcggtaag ccggataagg
      601 cgctcgcgcc gcatccggca acgatgccga ctgcctgatg cgacgcttgc gcgtcttatc
      661 aggcagcctc tcatctgtat aaatttcgaa ctacacttaa ctggcttctc ttaactgagg
      721 tcaccatcat gccgttaagt ccctacctct cttttgccgg taactgtgcc gacgcaattg
      781 cttattatca acgtacgttg ggcgctgaac tgctctataa aatcagcttc ggcgaaatgc
      841 caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt cccgataccg
      901 ccatcgccca cgccaatgtg cgcattgccg gaagcgacat catgatgagc gatgccatgc
      961 cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa caggtcgaag
     1021 aaggaaaacg ctggtttgac aatcttgccg ctaacggaaa aatcgaaatg gcctggcagg
     1081 aaactttctg ggcgcatggc tttggcaaag tcaccgataa atttggcgta ccgtggatga
     1141 ttaatgtcgt caaacaacaa ccaacgcaat aacccgccgg gaggcccgcc ctcccgcact
     1201 gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc aaatcgtcac
     1261 aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa acgattatcc
     1321 gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc gctgcatgcg gttgatctga
     1381 acattcatca cggtgaaatg gtggctctgc ttgggccgtc gggttccggc aaatccaccc
     1441 ttttacgtca cttaagcggt ttgattaccg gcgataaatc cgccggcagc catatcgagc
     1501 tgctgggccg cacagtccag cgcgaaggcc gtctggcgcg cgatatccgc aaaagccgcg
     1561 ccaacaccgg ctacatcttc caacaattca acctggtgaa ccgcctgagc gtactggaga
     1621 acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt agctggttta
     1681 cccgcgagca gaaacaacgc gcgttacagg cgctgacccg cgttggcatg gtgcattttg
     1741 cccatcaacg cgtttccacc ctctccggcg gacagcagca gcgtgtggcg attgcccgcg
     1801 cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg ctggacccgg
     1861 aatccgcccg catcgtgatg gacaccctgc gcgacatcaa tcagaacgac ggcatcaccg
     1921 tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc
     1981 tgcgccaggg gcacgttttc tacgacggca gcagccaaca gtttgataac gaacgttttg
     2041 accatctcta ccgcagcatt aatcgcatcg aagagaacgc gaaagctgcc tgacatcccc
     2101 atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc accagcatgt
     2161 tcagcctcag caccctgtta agcccggcac acgccgaaga gcaggaaaag gcgctgaatt
     2221 tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg ccattcttac
     2281 aggatatgga gaagaagctg ggcgtgaagg tgaacgcctt ctttgcccca gactacgcag
     2341 gcattatcca gggaatgcgc ttcaataaag tggatatcgc ctggtacggc aacctgtcgg
     2401 caatggaagc ggtggatcgc gccaacggcc aggtcttcgc ccagacggtc gcggcggatg
     2461 gatcgccagg ttactggagc gtgttgatcg tcaacaaaga tagtccgatc aacaacctga
     2521 acgatctgct ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct aactccacct
     2581 ctggcttcct cgtccccggt tactacgtct ttgccaaaaa caatatctcc gccagcgact
     2641 ttaagcgcac cgtcaacgct gggcatgaaa ccaacgcgct ggccgtcgcc aacaagcagg
     2701 tggatgttgc caccaacaac accgaaaacc tcgacaagct gaaaacctcc gcgccagaga
     2761 agctgaaaga actgaaggtg atctggaagt cgccgctgat cccaggcgat ccgatcgtct
     2821 ggcgcaagaa tctctccgaa accaccaaag acaagatcta cgacttcttt atgaactacg
     2881 gaaaaacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggccccg ttccgcgcct
     2941 ccagcgacct gcaactggtg ccgattcgcc agctcgcact gtttaaagag atgcagggcg
     3001 taaaaagcaa taaaggactg aatgagcagg acaagctggc gaaaaccacc gagattcagg
     3061 cgcagctgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt tcggtgagta
     3121 aagcggtgca gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc gacaatgtgc
     3181 aggcgttgat gccggatgcg gtgcaagcac cttatccggc ctacagaccg gagccaaaca
     3241 tgcaaaccat caccatcgcc ccacccaagc gtagctggtt ctcgcttctg agctgggccg
     3301 ttgttctcgc cgtgctggtc gtctcgtggc agggcgcgga aatggccccg cttacgctga
     3361 ttaaagacgg cggcaacatg gcaaccttcg ctgccgactt cttcccgccc gatttcagcc
     3421 agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgtc tggggcaccg
     3481 cgctggcagt ggttctctcc atctccttcg gcctgatgag cgccgaaaac ctggtgccgt
     3541 ggtgggttta ccagcccgtt cgccgcctga tggacgcctg ccgcgccatt aacgaaatgg
     3601 tcttcgccat gctgttcgtg gtcgccgtcg gtctcggacc gttcgctggc gtgctggcgc
     3661 tatttattca caccaccggc gtgctctcga aactgctttc cgaagcggtg gaagcgattg
     3721 aacccggccc ggtggaaggc attcgcgcca ccggcgccaa caagctggaa gagatccttt
     3781 acggcgtgct gccacaggta atgccgctgc tgatctccta ctccctctat cgcttcgaat
     3841 ccaacgtccg ctcggcgacc gtcgtcggca tggtcggtgc aggcgggatc ggcgtcaccc
     3901 tgtgggaagc gattcgcggt ttccagttcc aacaaacctg cgccctgatg gtgcttatca
     3961 tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac tttatctgat
     4021 aagcgaggca ttgatatcta tgcacttgtc tacacatccg accagctacc caacacgcta
     4081 tcaagagata gccgcaaaac ttgagcagga gcttcgtcaa cactatcgct gcggcgacta
     4141 tcttcccgcc gagcagcaac tggcggcgcg ctttgaggta aaccgccaca ccctgcgccg
     4201 cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggca tcggcgtgct
     4261 ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta gccagaacct
     4321 gctggaccag ggcagccatc ccaccagcga aaaactgctg tcggtattgc gccccgcgtc
     4381 cggtcacgtt gctgacgcgc tggggattac cgagggtgag aacgttatcc atttgcgcac
     4441 cctgcgtcgc gtcaacggcg tcgcgctctg tttaatcgac cactactttg cggacctcac
     4501 cctctggccg acgctgcaac gcttcgacag cggttcgctg cacgattttc tgcgcgagca
     4561 aaccggaatt gcgctgcgcc gcagccagac gcggatcagc gcccgccgcg cccaggccaa
     4621 agagtgccag cgccttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc gcacccttaa
     4681 ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc gcgccgacat
     4741 gattgaattc actatggagc actgaatgca cgcagatacc gcgacccgcc agcactggat
     4801 gtccgtactg gcgcacagcc aaccggctga actggcagcc cgcctgaacg cgctgaacat
     4861 caccgccgac tatgaagtga tccgcactgc cgaaactggc ctggtgcaga ttcaggcgcg
     4921 aatgggcggt accggcgaac gcttttttgc cggcgacgcc acgctgaccc gcgccgccgt
     4981 acgcctgact gacggcaccc tcggctacag ctgggtgctg gggcgtgata aacagcacgc
     5041 cgaacgctgc gcgctgattg atgcgctgat gcagcaatct cgccactttc aaaacttatc
     5101 agaaaccctt attgccccgc tggacgctga ccgtatggca cgcattgccg cacgccaggc
     5161 cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagaca acgcatgacc
     5221 ctggaaaccg cttttatgct tcccgtgcag gatgcccagc acagttttcg tcgcctgtta
     5281 aaggccatga gcgagccggg cgtgattgtc gccctgcatc agctcaaacg cggctggcag
     5341 ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga cacgccggtg
     5401 tggcttgcca cgccattaag taacgatatc gtcaatcaga gcctgcgttt tcataccaac
     5461 gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg tgacggataa ggcgatttcc
     5521 agcgaacagc tcaacgccct ttccaccggc accgccgttg cgccggaagc gggcgcgacg
     5581 ctgattttac aggtcgccag cctgagcggc ggacgcatgt tgcgtctcac cggcgcgggc
     5641 attgccgaag aacgaatgat cgctccgcag ctgccagagt gcattctgca cgaactcacc
     5701 gagcgcccgc acccgttccc gctcggcatc gacctaatcc tgacctgcgg cgagcgcctg
     5761 ctggctatcc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc gtgaaagggg
     5821 gcgaaaaggc gatcgacgcc gcccacgccc tgcaagagag ccgacgccgg ggcgataccg
     5881 atttgcccga actgagcgtc gcccagattg aacagcagct taacctcgcg gtggatcgcg
     5941 tgatgacaga aggcggcatt gccgaccgcg aactggcggc gctggcgctg aaacaggcca
     6001 gcggcgataa cgttgaagca attttcctgc tgcgcgccta ccgcaccacg ctggcgaaac
     6061 tggcggtaag cgagccgctc gacaccacag agatgcgtct cgaacggcgt atttcggcgg
     6121 tttataaaga cattcccggc ggccagctgc ttggcccaac ctacgactac acccatcgcc
     6181 tgctcgattt caccctgctg gcaaacggtg aagcgccgac gctgaccacc gccgacagcg
     6241 aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg gcgaagtttg
     6301 aagaagatag cggcgcacag ccggatgaca tcacccgcac gccgccggtt tacccctgct
     6361 cacgctcctc ccgcctgcaa caattaatgc gcggggacga aggctatttg ctggcgctgg
     6421 cctactccac ccagcgtggt tacggacgca atcacccgtt cgcgggcgag atccgcagtg
     6481 gttacatcga cgtgtcgatt gtgccggaag agctgggatt tgcggtaaac gtcggcgaac
     6541 tactgatgac cgagtgtgaa atggtcaacg gttttatcga cccgccggat gagccgccgc
     6601 acttcacgcg cggctacggg ctggtattcg gcatgagcga gcgcaaagcg atggcaatgg
     6661 cgctggtcga tcgtgcgttg caggctccgg aatacggcga gcacgcgaca ggcccggcgc
     6721 aggatgaaga gtttgtgctg gcacatgccg acaacgtcga agccgcaggc tttgtctcgc
     6781 acctcaaact cccccactac gtcgatttcc aggccgaact ggagctactc aaacgtctgc
     6841 aacaggagaa gaaccatggc taatctgagc ggctacaact ttgcctacct cgacgagcag
     6901 accaaacgca tgatccgccg cgccatctta aaagcggtgg cgatccccgg ttatcaggtg
     6961 ccgtttggcg ggcgcgagat gccgatgcca tacggctggg gaaccggcgg catacagctc
     7021 accgccagcg tgattggcga aagcgacgtg ttgaaggtga ttgaccaggg tgcggatgac
     7081 accaccaacg ccgtgtcgat tcgcaacttc tttaagcgcg tgaccggggt aaacaccact
     7141 gaacgtacgg acgatgcgac gcttatccag acgcgtcacc gcatccccga aacgccgctg
     7201 accgaagatc agatcattat cttccaggtg ccaatcccgg aaccgctgcg ctttatcgag
     7261 ccgcgcgaaa cggaaacccg caccatgcac gcgctggaag agtacggcgt gatgcaggtg
     7321 aaactgtatg aagatatcgc ccacttcggt catatcgcca ctacctacgc ctatccggtg
     7381 aaggtgaacg ggcgctacgt aatggacccg tcgccgatcc cgaaattcga taacccaaaa
     7441 atggacatga tgcccgccct gcaactgttc ggcgcggggc gcgagaagcg catctatgcg
     7501 gtgccgccgt ttacccgcgt ggaaagtctc gatttcgacg atcacccgtt caccgttcag
     7561 cagtgggatg agccatgcgc catctgcgga tcgacccaca gctatcttga tgaagtggtg
     7621 ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg ccgccaacag
     7681 agcgaggcga aaaaccaatg aatcaaccgt tactttcggt caataacctg acccaccttt
     7741 acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccgggg gaagtgctgg
     7801 gcattgtcgg ggagtccggc tccgggaaga ccacgctgct gaaatcaatc tccgcgcgcc
     7861 tgacgccgca gcagggggag attcactatg agagccgttc gctgtacgca atgagcgagg
     7921 ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtagt gcatcagcat ccgctcgacg
     7981 gcctgcgccg ccaggtgtcg gcaggcggca atatcggcga gcggctgatg gcgaccgggg
     8041 cacgtcatta cggcgatatt cgtgccaccg cgcagaagtg gctggaagag gtggaaattc
     8101 ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggcatgcag caacgtttgc
     8161 agatcgcccg caacctggtg acgcatccga agctggtgtt tatggacgaa ccaaccggcg
     8221 ggctggatgt gtcggtgcag gcgcgcctgc tcgatctgct gcgcggcctg gtggtggagc
     8281 tgaacctggc ggtggtgatt gttacccacg atttgggcgt cgcccgcctg ctggcggacc
     8341 gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac cgcgtgctcg
     8401 acgacccgca ccatccgtat acccagctgc tggtgtcatc ggttttgcag aattgaatgc
     8461 aacaccgtgc gcgctgatcc cctcaccccg gccctctccc ggagggagag ggagaaaacc
     8521 ggcgcaatat tcaacaccgc gcacaatcgg tcccctcgcc ccttcgggga gagggttagg
     8581 gtgagggcaa tgcgcgcacc gctccccaat atgcgaacca cgaggcgaaa aatgattaac
     8641 gtacaaaacg tcagtaaaac cttcatcctg caccagcaaa acggcgtgcg cctgcccgtc
     8701 ttacaacgcg cctcgctcac cgtcaacgca ggcgaatgcg tggtgctcca cggtcactcc
     8761 ggcagcggca aatcaaccct gctgcgctcg ctgtacgcca actatctacc cgacgaaggt
     8821 caaatccaga tcaaacacgg tgacgagtgg gtagacctgg tcaccgcgcc agcgcgcaaa
     8881 gtggtggaaa tccgcaaaac caccgtcggc tgggtgagcc agtttctgcg cgtcatcccg
     8941 cgtatctcag cactggaagt ggtgatgcag ccgctgctcg atacaggcgt tccgcgtgaa
     9001 gcctgcgccg ctaaagccgc gcgtcttctc acccgcctga acgtgccgga acgcctgtgg
     9061 cacctggcac catcgacatt ttccggtggc gaacagcagc gcgtcaacat cgcccgcggc
     9121 tttatcgtcg actaccccat tctgctgctc gacgaaccta ccgcctcgct ggacgccaaa
     9181 aacagcgccg cggtggtgga actgattcac gaagccaaag cccgtggcgc agccatcgtc
     9241 ggcatcttcc atgacgaagc tgtacgtaat gacgtcgccg accgcctgca cccaatggga
     9301 gcctcttcat gattatcaat aacgttaagc tggtgctgga aaacgaggtg gtgcacggtt
     9361 cgctggaggt gcaggatggc gaaatccgcg cctttgccga aagccagagc cgcctgccag
     9421 aagcgatgga cggcgaaggt ggctggctac tacccggcct gattgagctg cataccgaca
     9481 acctcgataa attcttcacc ccgcgcccga aagtggactg gcctgcccac tcggcgatga
     9541 gcagccacga tgcgctgatg gtggcgagcg gcatcaccac cgtgctggac gcggtggcga
     9601 ttggcgacgt gcgcgacggc ggcgatcggc tggagaatct ggagaagatg atcaacgcca
     9661 tcgaagagac gcagaaacgc ggcgtcaacc gcgccgagca ccgcctgcat ctgcgctgcg
     9721 aactgccgca tcacaccacg ctgccgctgt ttgaaaaact ggtgcagcgc gagccggtga
     9781 cgctggtgtc gctgatggat cactcgccgg gccagcgcca gttcgccaac cgcgagaagt
     9841 atcgcgaata ttatcagggc aaatactcgc tcaccgatgc gcagatgcag cagtacgaag
     9901 aagagcagct ggcgcttgcc gcacgctggt cacagcctaa tcgcaaatcc atcgccgccc
     9961 tgtgccgcgc acgacaaatt gccctcgcca gccacgatga cgccacgcat gcccacgttg
    10021 ccgaatccca ccagcttggc agcgtgatcg ccgaatttcc caccacgttc gaagcggcgg
    10081 aagcttcgcg caggcatggt atgaacgtac tgatgggcgc gccgaatatc gtgcgcggcg
    10141 gttcgcactc cggcaacgtg gcggccagtg aactggcgca gcttggcctg ctggatatcc
    10201 tctcttccga ctactacccc gccagcctgc tggatgcggc gttccgcgtc gccgatgacg
    10261 agagcaaccg ctttacgcta ccgcaggcgg tgaggctggt gacccaaaat ccggcgcagg
    10321 cgctcaatct tcaggatcgc ggggtgattg gcgagggtaa acgcgctgac ctggtgctgg
    10381 cgcatcgcca aggcaatcac attcatatcg accacgtctg gcgtcagggt aaaagggtgt
    10441 tctgatgatg ggaaaactga tttggttaat ggggccgtcc ggctccggga aagacagcct
    10501 gctggcggaa ctccgcctgc gggaacaaac gcagttactg gtggcgcatc gctacatcac
    10561 gcgcgacgcc agcgccggaa gtgaaaacca tatcgccctg agtgagcggg agttttttac
    10621 ccgcgcgggg caaaacctgc tggccttaag ctggcacgct aacggtctgt attatggcgt
    10681 cggcatcgag attgacctct ggctgcacgc tggattcgac gtagtcgtca acggttcacg
    10741 cgcgcatttg ccgcaggcac gggcgcgcta tcaatcggcg ctgctgcccg tctgtttaca
    10801 ggtttcgccg gagatcctgc gccagcgcct ggaaaaccgt ggccgtgaaa acgccagtga
    10861 aattaacgcc cgcctggcgc gcgccgcccg ctatactccc caggattgcc atacgctcaa
    10921 caatgacggc agcctgcgcc agtcggtcga cacgctgctg acgctgatcc atcagaagga
    10981 gaaacaccat gcctgcttgt gagcttcgcc cggccacgca gtacgatacc gacgcggttt
    11041 acgcgctgat ttgtgagcta aaacaggcgg agtttgacca ccatgcgttt cgcgtgggct
    11101 ttaacgccaa cctgcgcgac ccaaacatgc gctaccatct ggcgctgctg gatggcgaag
    11161 ttgtcggcat gatcggcctg catttgcagt ttcatctgca tcatgtcaac tggatcggcg
    11221 aaattcagga actggtggtg atgccgcagg cgcgcggtct gaacgtcggc agtaaattac
    11281 tggcatgggc agaagaagaa gcccgccagg ccggggccga aatgaccgag ctttccacca
    11341 acgtgaagcg ccacgacgca caccgttttt acctgcgtga aggctacgag cagagccact
    11401 tccgcttcac caaggcgctg taacatgagc ctgaccctca cgctcaccgg cactggcggc
    11461 gcacagggcg ttccggcatg gggctgcgag tgcgcggcct gcgccagagc gcggcgctcg
    11521 ccgcagtatc gccgccagcc atgcagcggc gtagtgaagt ttaacgacgc gatcaccctg
    11581 atcgacgccg ggcggcacga tctcaccgat cgctggtcgc ctggatcgtt ccagcagttt
    11641 ttgctgacgc actaccatat ggatcacgtc caggggctat ttccgctacg ctggggcgtg
    11701 ggcgatgtga tcccggtcta cggcccgccg gatgaacagg gctgcgacga tctgtttaaa
    11761 catccgggcc tgcttgattt cagccacaca gtggaaccgt ttgtggtgtt tgatttgcag
    11821 gggttgcagg tcacgccgct gccgcttaac cactcaaaac tgaccttcgg ttatctgctg
    11881 gaaacggcac atagccgggt ggcgtggctg tctgacaccg ctggcctgcc ggaaaaaacg
    11941 ctgaaatttt tactcaataa tcatccgcag gtgatggtga ttgattgcag tcacccgccg
    12001 cgcgcggatg caccgcgtaa tcactgtgat ttaaataccg tgcttgcgct gaatcaggtt
    12061 atccgctcgc cacaggtgat tctgacccat atcagccacc agtttgatgc gtggctgatg
    12121 gaaaacgcac taccgtcagg gtttgaggtg gggtttgatg ggatggagat tggggtggcg
    12181 tgatgagagg gaatgtgcgc gctggccccc tcaccctaac cctctcccca gaggggcgag
    12241 gggaccgatt gtgctcgata ttgaatattg cgctcgtttt ctccctctcc ccattggggt
    12301 gaggggcgat gcctgctcca tacccaacct catcgcccat actcatcttc cattctccgc
    12361 tcttcatcct ccagttgccg acgctcctga tcaagctggc gctggcgatc gtccagctgc
    12421 ctgcggcgat cttcaaactg gcggcggcgg tcgtcatatt gtctgcgccg atcgtcgctc
    12481 acttcacgct gccagccgtc gtcgcgcgaa tcttcatagt ctcgcccacg gtcagggtta
    12541 taagcgtcat taatcgcctg ctgaatatta ccaatagtat cgtcgataat atcagcctgg
    12601 gccggaacgg tggacagcgt gagcagggtg aataaaagaa atagcggaaa gcgtttcatt
    12661 agccaacctc aaaaagaaac tctatccaca ttagtcatta ctcatccatg caagtagtgg
    12721 atgaatctca atttctccgc tgctctattg ccgtaatcgc ctccacgcgt tgttgatgac
    12781 gaccgccttc gtactgtgcg cccagccacg catccacaat catttttgcc agttcgaggc
    12841 caaccactcg tgaaccaaaa gccagcacgt tggtgtcgtt atgctgccgc gaaagttgcg
    12901 cggaataagg ttcgctacag acgaccgcgc gaattccggc aaacttgttc gccgctatcg
    12961 aaataccgac gccagtacca caaatcaaaa tcccgccatc aacctctccg ccagcaacag
    13021 ccagtgcgac ttgactggcg taatgcggat aatcagtacg ctctgacgac caggttcctt
    13081 tatcaatcac ttcaacgcca cgctcaacta aatgtgccac tatttcatgt tttaaaatga
    13141 aaccgacatg atcacagcca aatgcaatct ttttcattca tcttccccgt tcgtgcaaaa
    13201 acttcacaat caaaaaacat ttgtgatgta aatgaattaa aaacacccaa aatcgatgaa
    13261 atccgccttc ctgatctgtt ttacagagtt acatctgaca gcgcaatagc gttaaagaca
    13321 cttactatcc agcaacatct atcatctaaa aaaccagaaa aacaaataac atcatgtttt
    13381 taaagtgatt gaatga
//
LOCUS       DAJSKJ010000109        13208 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000109 DAJSKJ010000000
VERSION     DAJSKJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.109"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..718
                     /locus_tag="ON345_002657"
     CDS             <1..718
                     /locus_tag="ON345_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3072695.1"
                     /translation="IMEKILFDNCILDDSNFAQIKMADGTLNACSAMHVQFYNAAMNR
                     ANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNL
                     NKAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKA
                     CMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQT
                     EDNINAVAMKITADIMQLAD"
     gene            complement(872..2521)
                     /locus_tag="ON345_002658"
     CDS             complement(872..2521)
                     /locus_tag="ON345_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR3072696.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2672..4021)
                     /gene="ghxP"
                     /locus_tag="ON345_002659"
                     /gene_synonym="yjcD"
     CDS             complement(2672..4021)
                     /gene="ghxP"
                     /locus_tag="ON345_002659"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR3072697.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4567..5031)
                     /gene="soxR"
                     /locus_tag="ON345_002660"
     CDS             complement(4567..5031)
                     /gene="soxR"
                     /locus_tag="ON345_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR3072698.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLIASIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5117..5440
                     /gene="soxS"
                     /locus_tag="ON345_002661"
     CDS             5117..5440
                     /gene="soxS"
                     /locus_tag="ON345_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR3072699.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRIFRRQFDRTPSD
                     YRHRL"
     gene            complement(5443..7029)
                     /gene="pdeC"
                     /locus_tag="ON345_002662"
     CDS             complement(5443..7029)
                     /gene="pdeC"
                     /locus_tag="ON345_002662"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR3072700.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQNRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7459..7740
                     /locus_tag="ON345_002663"
     CDS             7459..7740
                     /locus_tag="ON345_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR3072701.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMIFT"
     gene            complement(7839..8375)
                     /gene="ssb1"
                     /locus_tag="ON345_002664"
     CDS             complement(7839..8375)
                     /gene="ssb1"
                     /locus_tag="ON345_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR3072702.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8629..11451
                     /gene="uvrA"
                     /locus_tag="ON345_002665"
     CDS             8629..11451
                     /gene="uvrA"
                     /locus_tag="ON345_002665"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3072703.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11486..11842)
                     /locus_tag="ON345_002666"
     CDS             complement(11486..11842)
                     /locus_tag="ON345_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR3072704.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11846..12262)
                     /locus_tag="ON345_002667"
     CDS             complement(11846..12262)
                     /locus_tag="ON345_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR3072705.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12373..13086)
                     /gene="aphA"
                     /locus_tag="ON345_002668"
     CDS             complement(12373..13086)
                     /gene="aphA"
                     /locus_tag="ON345_002668"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR3072706.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3439 a         3452 c         3209 g         3108 t
ORIGIN      
        1 cataatggaa aaaatacttt ttgataattg tattctcgat gactcaaatt tcgctcagat
       61 aaaaatggcc gacggaactc taaatgcatg ctccgctatg catgttcaat tctacaatgc
      121 agcaatgaat agagccaata ttaaaaatac ctttcttgac tattcaaatt tttatatggc
      181 gtacatggct gaggtaaatc tttataaagt aatagcgcca tatgttaatt tatttaaagc
      241 cgaccttagt ttctctaaac tcgatttaat taactttgaa catgctgatc tgtctcgcgt
      301 caatctgaac aaagcaatcc tccagaatat aaacttaatt gatagcaaac tcttttgtac
      361 gtggctaaca aatacattcc tcgaaatggt tatatgtacc gactctaata tggctaatgt
      421 taattttaat aatgccaatt taagcaactg ccatttcaac tgttctattt taacaaaagc
      481 ctgtatgttt aatacccgtc tctatcgggt taattttgat gaggctagcg tccagggaat
      541 gggcatttcc atactccgtg gggaggaaaa tatccccatt gatagtgata ccctggtaac
      601 actacagaaa ttctttgaag aagattgtac ctctcatact ggcatgtcac aaactgagga
      661 taatattaat gcagtcgcta tgaagattac tgcagatatt atgcaactcg cagattgagg
      721 ttaagtcgaa gaacagccaa tgaattgact ttacaagaga gataacttat ttgttttaga
      781 atcatgaatg ttagcccgag ctaacctgtt aaaggtgcaa aagattgcac ctttttcaga
      841 ttacctcggg cctggttacg tcgctttcgg cttactgatt ttcctcaatt agcaacgctt
      901 caagcaaatc gagatcgtgc agtaattttt gcagcgtttc gttgctgatc tcccgcgtgg
      961 cgcgcaggtg gtaaagttca gcacgttcag aacgcaatgc cgccagacgg aagcgacgct
     1021 caaggttctc ttcctgcacg gaactttcaa cgtcgttacg tccatcggcg cgacgacgca
     1081 ggttaccaat gacgcgagaa ctgacctccg taagcagctg gttatcgata ttctcttcgg
     1141 tatccgccgc cagacgttcc tccattttct ggatcgccac aatcgccact tctgccgttg
     1201 ccgctcgcgc aatacgttct tctttcaatt gttgcgaatg gtccgcgact tcaatgtgtt
     1261 gtagcagaat aggcaacatc accacgccga caaacagcga gaagagaatg acgccagccg
     1321 ccaggaacac cagctcatag cgcgccggga agacgttacc atccggcaag agcagcggga
     1381 tagagagcac accggccaga gtgatcgccc cacgcacccc ggcgaaagac gcgatcagga
     1441 tttctcgtgt ggtccacgaa ccaaactcca tcggcttctt cttcaggaag cggttgctga
     1501 actttttcat cgtccacagc cagccgaaac ggaccagcat cagcgccgca tatatcagaa
     1561 taatatcggt aaacagcatc cagatttcga cgttagggtc gatttctgcc gccatcagcg
     1621 acgtctccag aatacccggc agctgcagac ctaacagcag gaacaccatg ccgttaaaga
     1681 caaattccag catcgcccag gtgctgtttg cgcgcaggcg cattgccagc ggcgcacggc
     1741 gcatcacacc ggagcgggtg atggtcatcc cggcggcaac cgcagcgagg atgcccgaaa
     1801 cgccaatatg ttcggcaatc agataagaag cgaatggcag caagaacagc agaacaatct
     1861 gcgtcgcggg ttcatcaccg ccccagcggc tgaggaatcg cagcgagcga ccatacagcc
     1921 agctcaccac aaaaccggca agaataccgc caatggctac tttcataaat tcgaccgtcg
     1981 caccgccgac ggtgaagatc atcgtcccca tcgccactgc cacggcaaac ttcaacgaca
     2041 ccaggccgga agcgtcattc atcaacgctt cgccctgcaa aatgcccatg atttttttcg
     2101 ggatgcgccc ttcgccaaca atcccggaga gcgccacagc atcggtcgga gaaagtaccg
     2161 ccgccagcgc aaaggcgggg atcagcggaa tgcccggcac cacccagtaa ataaggaagc
     2221 caatgccgac cacggtgacc accaccagcg ccagcgcgag gccgaaaatc tctcgaccat
     2281 gttcgagaaa ttcacgggtc ggcgttttcc agccatcagc gaacagcaac ggcgggataa
     2341 acaagactaa aaagagttca ggatcaaact ccacatgcaa accaaacgtc ggccacgcca
     2401 gtagcgcacc gatggcgatt tgcataagcg gaagcgggat ctgaaagggc atgacacgag
     2461 tgaccacccc ggacagcgag accacgaggg tcattatcag tatggtgaag aagatttcca
     2521 tgcgttccct gtttgcgatg ttttttatga tctgagaaag acgttatgcc tgaattctca
     2581 ttttttcaga atatcgcaaa agctcacaca atgtgcggag aaaataaaaa cgggcagcca
     2641 ttgccacccg ttttcagaat ccgatgaaag attagatagc ccagccaccc gcatagaagg
     2701 tcaccagcgc cacggcgata acgaccgtac cgatgttcaa cttgcgccat tcgccggaaa
     2761 ccagacgacc aatcaccaga gtcgcgaagc cgatcatgat gcctgttacg atgttacagg
     2821 tcagcacgat gaataccgcc gtaaccagac ccgccatcgc atcaacaaaa tcagcaaagt
     2881 cgattttcgc cacgttgctc agcatcagca ggccaacgta catcagcgcc ggagccgttg
     2941 cataccccgg aacgaggtaa gagagcggag agaggaacag aatcagcagg aacagcacgc
     3001 caacggtgat agccgtcaaa ccggttttac cgcccgccgc cgtacccgcc gcagactcga
     3061 tgtataccgc tgccggagct gcacccacca ggccagagaa aacgctgctc atggagtcag
     3121 tggtcagtgc tttcccaccg tcgataattt gcccatcttt atccagcagg ttcgcctggc
     3181 cggcgacggc acggatagtt ccggtggcat caaatactgc cgtcattacc agcgccagaa
     3241 cgcttggcag gactacagga ttcagcgcgc ccataatgtc caggctgcca atcagtgagt
     3301 tgccgttttc atcgctcaat gaaggcatgg cgaaaacgcc ggagaaatgg acgttaggat
     3361 cgaagatcaa accgacaatt gagataccga taatggtcag cagaatgcca ccagggactt
     3421 tcagtttttc caggccgatg atcaccgcca gacctaccag tgacataatc accgggaagg
     3481 tcgcgaaatc acccagcgca acgggcagac catcaagcgg gtttttaatc accagaccga
     3541 caccgttagc ggcaatgagc agcaggaaca gaccgatacc aatccccgtg ccgtgcgcca
     3601 caccgtgcgg caagttgcgc aaaatccagc tacggatacc cgtggcagaa attaccgtaa
     3661 acagcacacc catcaggaac acggcaccca gcgcgacagg tacgctaata tgttgcccca
     3721 gcaccaggct gaatgcggta aatgccgtca gggagatggc gcaaccaatc gccaacggca
     3781 gattagccca cagacccatc acgatagaac cgagtccggc aaccagacag gttgcaacga
     3841 aaactgccgc aggcgggaag cctgctttac ccaacatacc tggaacgacg atgaccgagt
     3901 agaccatcgc cagaaacgtt gttaacccgg caaccacttc ctgacggaca gtgcttccac
     3961 gttgtgaaat tttaaaccag gcgtcgagtg aaccgccggt acgcgctgat ggcgtagaca
     4021 taagaaaaca tcccctgaga gtttaatttt cgtcaagatg cgtggcggac aatcccctgc
     4081 cagcgaaacg ttatcttcct gctccaggtt tgcgacaatt tgctgcgtcg caaaaaagca
     4141 aacgtttagc tccgcgcata caaaacgggt ggtaaaaaaa gcaaacgatt atccggcgat
     4201 catagcggct ttttcaaccg aagtttgctg cttttttctc ttttattgtc ttgacgatga
     4261 aaaaccaggc aattgatgcg caaacgttat cgtcggtgcg caagattgca gcgttatcga
     4321 gccgtatacc gttcaggatt gcgataaaaa atcgtctgag atgacaaaaa tattgcggtt
     4381 aaaaagtata cttttacact ggcgaagaaa cgtcggtgac atacaattaa agcatcaaca
     4441 ccaaccggaa cctccaccac gtgctcgaat gaggtgtgtt gacgtcgggg gaaaccctcc
     4501 tgtgtaccag cgggatagag agaaagacaa agaccggaaa acaaactaaa gcgcccttgt
     4561 ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt ctcctaagcg
     4621 gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac caatacatcc
     4681 gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc cgatccaact cttctcgcca
     4741 ttgggacgaa agctgtttcc actctttcgc acttaacgta tgcccttcgg gcaacacgcc
     4801 aaacgcttca ccaatggtcg ccagcggaat gccaatacgc tgagcaattt tgataattgc
     4861 aacatatcgc aacacatcac gtttatatcg ccgctgattg ccgctgttac ggatactggc
     4921 aatcaaccct ttactttcat agaaatgcag cgccgatacc gccacaccgc tgcgtttcgc
     4981 cacttcgccg ggggttagca gcgctttaat gcggggtaat ttcttttcca taaatcgctt
     5041 tacctcaagt taacttgagg aattatactc cccaacagat gaattaacga actgaacact
     5101 gaaaagaggc agatttatgt cccatcagaa aattattcag gatcttatcg catggattga
     5161 cgagcatatt gaccagccgc ttaacattga tgtagtcgca aaaaaatcag gctattcaaa
     5221 gtggtacttg caacgaatgt tccgcacggt gacgcatcag acgcttggcg attacattcg
     5281 ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc accgagcgtc cgatttttga
     5341 tatcgcaatg gacctgggtt atgtctcgca gcagaccttc tcccgcattt tccgtcggca
     5401 gtttgatcgc actcccagcg attatcgcca ccgcctgtaa ttttattgcc cgcgcgttaa
     5461 ctcccgcgcg ggtaattgct ccatccattg cataaacacc tgcggcggca tcgccttcgc
     5521 aaagaaccat ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa cctgctcctc
     5581 agtttcgacg ccttcagcga tcgtttttaa ccccaggctg tgcgccagct cgatgatgtg
     5641 ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg atttgtcgat
     5701 tttcaaaata tcgacattca atgatttaag gttatgcaag ttagagtagc caataccaaa
     5761 atcatcaatt gccacttcgt aacctgcctg gcggaaagcc agaataatcg gcgtcatttt
     5821 gtcaacatca agaaatgcat gttcagtcac ttcaaattta atttgctgcg gacgcaccgc
     5881 gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa aatcggaggc
     5941 cgacaggtta atagaaacat agcgatctgc atgtgttgcc aggtaatcgc ccagatcgcg
     6001 gaagacatta tcaataacat aatcagttac ctgttctatc atcccctcct tttctgccag
     6061 cggaataaac tctgccggat tcattatttg cccctgctca ccaagccaac gtaacaacgc
     6121 ttcagcgccg atacattttt ctgttttgat atcgattatt ggctggtaat aaagacaaag
     6181 ttgatgtttt tcgagggcgc gttgcaattt acgtttggga gataaatagt tttgtcgaat
     6241 acgtagccag agtaatagca gaacaagact ccccaaaata ccggcgggca acgcaaaaat
     6301 aagatgatta taaaaatggg ttataagacg ttgatatgaa gtcgcaacaa tggctgcaat
     6361 tgggcgtttt gtcgaataaa ctgtcgcata taaatagcca tttctttgta cggttaaatc
     6421 cttcaaatga atcagcggag aaaacgttgc tgccgaggcc tctttgctta acgagaaaaa
     6481 ggttttcgtc accgtatcat acacacccca ttgcaatgtc gggtcatcag acatcacttc
     6541 actccagaag agagggttga taaccgccac ataatttccc cgctgcatat aggtcatttt
     6601 atagccagag aaaaaaggcg tatcgcggta ataatagata gaaacgttag gttcacgctt
     6661 ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct atcagcgatg agcataaaaa
     6721 atggttatca cgggcataga tcaattcatt aatataaaga tagccacgaa taatattcaa
     6781 cattcgattt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag ctgcatcgct
     6841 cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt gagcaaaggt
     6901 tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata gcgaaagaat
     6961 gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt gtcgtgcacg
     7021 atgactcatt gctatcaatt tcctgcctgt ttatacccgg ccattgcaag gtgaaaaccc
     7081 tgggtaccga cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa
     7141 attgatacct ttctaatcat cacctggatg aaaataattc attgatattc aaaattaatt
     7201 tcctacagga atgcagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat
     7261 aatctgtaca gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat
     7321 agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg
     7381 atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtccaggg catcggctct
     7441 tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct tcgctggcaa
     7501 cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt tcgtcggtct
     7561 gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg ttgtttccgc ctgttggttc
     7621 gcaatggggt tgcttggcat cacaatggcg gacatcaccg ctatctggca taacatcgag
     7681 tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat tttcacctga
     7741 tacgtagaag cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc
     7801 caccttaaaa caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca
     7861 aagtccatcg gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc
     7921 gcgccgccgc tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc
     7981 ggctgaccac caccgatatt accacctgcc ggagcgccac caccctgacg accacccagc
     8041 atctgcatgg tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat
     8101 tgatcggtcc atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc
     8161 agatattcgc tggccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc
     8221 tgttctttca tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg
     8281 ttggcaactg cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca
     8341 acgagaataa ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt
     8401 tctgaataag tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga
     8461 cctcggttcc gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat
     8521 tccaatactg tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg
     8581 gtagcaccat gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc
     8641 gaagttcggg gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac
     8701 aagctcattg tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc
     8761 ttatatgccg aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt
     8821 ctgtcactga tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca
     8881 attgagcaga aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc
     8941 cacgactatt tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac
     9001 gtcccgctgg cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa
     9061 ggcaagcgtc tgatgctact cgcgccaatc attaaagagc gcaaaggcga acacaccaaa
     9121 acgctggaga acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc
     9181 gatctttccg atccgccgaa actggaactg caaaagaaac acactattga agtggtggtt
     9241 gatcgcttca aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg
     9301 ctggagcttt ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag
     9361 ctgctgttct ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag
     9421 ccgcgactgt tttcgtttaa caacccggca ggtgcctgcc caacctgtga cggccttggc
     9481 gtacagcaat atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc
     9541 ggtgcgatcc gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg
     9601 gcagatcact ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat
     9661 aaagtggtgt tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt
     9721 ggcgatacct ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt
     9781 tataaagaga cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc
     9841 ccatgcgcca gctgcgaagg gacgcgtctg cgtcgggagg cgcgccacgt ttatgtcgag
     9901 aatacgccgc tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac
     9961 aatctcaaac tcgcaggtca gcgggcgaag attgcagaaa aaatccttaa agagatcggc
    10021 gatcgtctga aattcctcgt taacgtcggc ctgaattacc tgacactttc ccgctcggca
    10081 gaaacacttt ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc
    10141 ctggttggcg ttatgtatgt actggacgag ccgtctatcg gcctgcacca gcgcgataac
    10201 gagcgcctgt tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg
    10261 gagcatgacg aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg
    10321 ggtgttcacg gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct
    10381 gaatcgttga ccgggcagta catgagcggt aaacgcaaga ttgaagtacc gaagaaacgc
    10441 gttccggcga atccagaaaa agtgctgaag ctgacaggtg cacgcggcaa caacctgaag
    10501 gacgtgacgc tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc
    10561 ggtaaatcga cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt
    10621 gcgaccatcg ccgaaccggc accgtatcgc gatattcagg ggctggagca tttcgataaa
    10681 gtgatcgata tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat
    10741 accggcgtgt ttacacctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt
    10801 ggttatacgc caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag
    10861 ggcgacggtg tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat
    10921 cagtgcaaag gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc
    10981 atccacgaag tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca
    11041 gctctggcgc gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg
    11101 cagtccgcaa ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg
    11161 tcaaaacgcg gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac
    11221 ttcgccgata ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc
    11281 attgtggtga ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg
    11341 ggaccagaag gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc
    11401 gcggagtgcg aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg
    11461 ccgctttctg agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg
    11521 gtagtaaatt cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg
    11581 gcagcgaacc gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac
    11641 gcacatcgct gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa
    11701 cagctgggcg attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct
    11761 gcgtcgcttt ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt
    11821 gtagcaactc cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg
    11881 atccgccgtg gatgcgatgt tcccccagcc agatgccttg ccaggtgccg gtctgaatac
    11941 gccctttatg taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag
    12001 gcatatcgtc tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga
    12061 ggaaaaaacg ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag
    12121 aggcggaggt atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag
    12181 ccagctgatt cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg
    12241 tgagcgtctt ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca
    12301 gcaagacagc gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcacgc
    12361 tctcccgctc tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg
    12421 tggcaagggt ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac
    12481 atcgcgtgcg gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc
    12541 ctgcagccat tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt
    12601 catgttggtg gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt
    12661 cgtcgggcta cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat
    12721 cagctggcga gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc
    12781 ccagaacaca ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg
    12841 ccagaacccc ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg
    12901 cggacgccct gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg
    12961 ttcagcaagc ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc
    13021 aacagcggaa ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt
    13081 gcgcatattt ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca
    13141 aaaaccatct gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt
    13201 gatgaaaa
//
LOCUS       DAJSKJ010000110        13113 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000110 DAJSKJ010000000
VERSION     DAJSKJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.110"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            228..1337
                     /gene="livK"
                     /locus_tag="ON345_002669"
     CDS             228..1337
                     /gene="livK"
                     /locus_tag="ON345_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR3072707.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1385..2311
                     /gene="livH"
                     /locus_tag="ON345_002670"
     CDS             1385..2311
                     /gene="livH"
                     /locus_tag="ON345_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR3072708.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2308..3585
                     /gene="livM"
                     /locus_tag="ON345_002671"
     CDS             2308..3585
                     /gene="livM"
                     /locus_tag="ON345_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR3072709.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDSSTVKQKLFLVALLVLAVV
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIMRILLLNNTEITCG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3582..4349
                     /gene="livG"
                     /locus_tag="ON345_002672"
     CDS             3582..4349
                     /gene="livG"
                     /locus_tag="ON345_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR3072710.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4351..5064
                     /gene="livF"
                     /locus_tag="ON345_002673"
     CDS             4351..5064
                     /gene="livF"
                     /locus_tag="ON345_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR3072711.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5461..6777
                     /gene="ugpB"
                     /locus_tag="ON345_002674"
     CDS             5461..6777
                     /gene="ugpB"
                     /locus_tag="ON345_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR3072712.1"
                     /translation="MKPLHYTASALALGLALMGNVQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKDSGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6875..7762
                     /gene="ugpA"
                     /locus_tag="ON345_002675"
     CDS             6875..7762
                     /gene="ugpA"
                     /locus_tag="ON345_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR3072713.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7759..8604
                     /gene="ugpE"
                     /locus_tag="ON345_002676"
     CDS             7759..8604
                     /gene="ugpE"
                     /locus_tag="ON345_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR3072714.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8606..9676
                     /gene="ugpC"
                     /locus_tag="ON345_002677"
     CDS             8606..9676
                     /gene="ugpC"
                     /locus_tag="ON345_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR3072715.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9673..10416
                     /gene="ugpQ"
                     /locus_tag="ON345_002678"
     CDS             9673..10416
                     /gene="ugpQ"
                     /locus_tag="ON345_002678"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3072716.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10403..10843)
                     /locus_tag="ON345_002679"
     CDS             complement(10403..10843)
                     /locus_tag="ON345_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR3072717.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10963..12705
                     /gene="ggt"
                     /locus_tag="ON345_002680"
     CDS             10963..12705
                     /gene="ggt"
                     /locus_tag="ON345_002680"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR3072718.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12743..13027)
                     /locus_tag="ON345_002681"
     CDS             complement(12743..13027)
                     /locus_tag="ON345_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HCR3072719.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2912 a         3344 c         3758 g         3099 t
ORIGIN      
        1 ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag aatattatct
       61 gttcttaatc gactgaaaaa tagggatttt aatcactatt atcacaaaat actgcgctaa
      121 acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct gatttgttaa
      181 cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg aaacggaatg
      241 cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct atggctgacg
      301 atattaaagt tgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg ggcgatatgg
      361 aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga attaaaggcg
      421 ataagctggt gggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc gttgcggtcg
      481 ccaacaaaat cgttaacgac ggcattaaat acgttattgg tcatctgtgt tcttcttcta
      541 cccaacctgc gtcagatatc tacgaagacg aaggtattct gatgatctcg ccgggagcga
      601 ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc gggctggact
      661 cttcccaggg gcctacggcg gcaaaataca ttcttgagac ggtgaagccc cagcgcatcg
      721 ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg caggacgggc
      781 tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg gagaaagatt
      841 tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac tacggcggtt
      901 actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg aaaactcagt
      961 ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc gatgctgccg
     1021 aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac cagggcatcg
     1081 ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg atcacctacg
     1141 cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag ccgctggcgc
     1201 tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg aactgggatg
     1261 aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc gatggttcat
     1321 ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt agaaaggtta
     1381 ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg tcacgctggg
     1441 cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta tcggcatgat
     1501 caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta tgatcatcgc
     1561 cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg gattcgtcgg
     1621 cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct atcgcccggt
     1681 gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca tcttcctgca
     1741 aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc tgtttaacgg
     1801 gcagtgggtg gtgggccata gcgaaaactt ctctgcctct attactacca tgcaggcggt
     1861 gatttggatt gttaccttcc tcgccatgct ggcgctgacg attttcattc gctattcccg
     1921 tatggggcgc gcctgtcgtg cctgcgcgga agatctgaaa atggcgagcc tgcttggcat
     1981 caacaccgac cgggtgattg cgctgacctt tgtgattggc gcggcgatgg cggcggtggc
     2041 gggtgtgctg ctcggtcagt tctacggtgt gattaacccc tacatcggct ttatggccgg
     2101 gatgaaagcc tttaccgcgg cggtgctcgg cgggattggc agcattccgg gggcgatgat
     2161 tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga gtacggaata
     2221 taaagatgtg gtctcatttg ccctgctgat cttggtactg ctggtgatgc cgaccggtat
     2281 tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat ggcgctgctc
     2341 tctgccgcga tgttctttat cctcgctggc gtctttatgg gcgtacagct ggagctggac
     2401 ggcaccaaac tggtggtcga cacggcttcg gatgtccgtt ggcagtgggt gtttatcggc
     2461 acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg gctgaaaagc
     2521 gtttccggac cgaagtttat tctgcctgca attgatagct ccacggtgaa gcaaaagctg
     2581 ttcctcgtgg cgctgctggt gctcgcggtg gtgtggccgt ttatggtgtc gcgtgggacg
     2641 gtggatatcg ccactctgac catgatctac attattctcg gtctcgggct gaacgtggtt
     2701 gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat cggcgcttac
     2761 acctttgcgc tgctcaatca ctattacggc ctgggcttct ggacctgtct gccgattgcc
     2821 gggttaatgg cagcggcggc cggcttcctg ctcggcttcc cggtgcttcg cttgcgcggc
     2881 gactatctgg cgatcgttac cctcggtttc ggcgaaatta tgcgcatatt gctgctcaat
     2941 aacaccgaaa ttacctgcgg cccgaacgga atcagtcaga tcccgaaacc gacactcttc
     3001 ggactcgagt tcagccgtac cgctcgtgaa ggcggctggg acacgttcag taatttcttt
     3061 ggcctgaaat acgatccctc cgatcgtgtc atcttcctct acctggtggc gttgctgctg
     3121 gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg gcgtgcgtgg
     3181 gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc gcgtcgtatc
     3241 aagctgaccg cttttaccat cagtgccgcg tttgccggtt ttgccggaac gctgtttgct
     3301 gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ccgaatcggc gtttgtgctg
     3361 gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc ggcaattttg
     3421 ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt aatgctcggt
     3481 ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat gacgcgcccg
     3541 caactgaagt tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag ccattattat
     3601 ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac gtcaatcttg
     3661 aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga aaaaccacgg
     3721 tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta ctgcgcaatc
     3781 agcacctgga aggtttaccg gggcagcaaa tcgcccgcat gggcgtggtg cgcaccttcc
     3841 agcatgtgcg tctgttccgt gaaatgacgg tgattgaaaa cctgctggtg gcgcagcatc
     3901 aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc cgtcgcgccc
     3961 agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg ctggaacacg
     4021 ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag attgcccgct
     4081 gcatggtgac gcagccggag attttaatgc tcgacgaacc tgcggcaggt cttaacccga
     4141 aagagacgaa agagctggat gagctgattg ccgaactgcg caatcatcac aacaccacta
     4201 tcttgttgat tgaacacgat atgaagctgg tgatgggaat ttcggaccga atttacgtgg
     4261 tcaatcaggg gacgccgctg gcaaacggta cgccggagca aatccgtaat aacccggacg
     4321 tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc ctttgacaaa
     4381 gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcttgca tatcaatcag
     4441 ggcgagattg tcacgctgat tggcgcgaac ggggcgggga aaaccacctt gcttggtacg
     4501 ttatgcggcg atccgcgcgc caccagcggg cgaattgtgt ttgatgataa agacattacc
     4561 gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga agggcgtcgc
     4621 gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt tgccgaacgc
     4681 gaccagttcc aggagcgcat aaagtgggtg tatgaactgt ttccacgtct gcatgagcgc
     4741 cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc gattggtcgc
     4801 gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgctcgg tcttgcgccg
     4861 attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg gatgactatc
     4921 ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg ctacgtgctg
     4981 gaaaacggcc atgtggtgct ctccgatacc ggcgacgcgc tgctggcgaa cgaagcagtg
     5041 agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa aagactcctt
     5101 tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc ctactgggtg
     5161 agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt aagccgcatc
     5221 cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt ttccgcttat
     5281 ccgtgcccca tctcccattt tccctcaccc gcgccgtcac cgccttgtca tctttctgac
     5341 accttactat cttacaaatg taacaaaaaa gttatttttc tgtaattcga gcatgtcatg
     5401 ttaccccgcg agcataaaac gcgtgaattc gcgcattcgg tacaacaaga gagataaacg
     5461 atgaaaccgt tacattatac agcttcagca ctggcgctcg gactggcgtt aatggggaat
     5521 gtacaggcag tgacgaccat tccgttctgg cattctatgg aaggggaact gggtaaagag
     5581 gtggattctc tggcccaacg ttttaacgcc gaaaacccgg attacaaaat tgtaccgacc
     5641 tataaaggca actacgaaca gaatttaagc gcggggattg ccgcatttcg taccggcaac
     5701 gcgccggcta ttttgcaggt ttatgaagtt ggcaccgcca ccatgatggc gtcgaaagcc
     5761 attaaaccgg tgtatgacgt gtttaaagag gcggggattc aattcgatga gtcgcagttt
     5821 gtgccgacgg tttcaggtta ctactccgac agcaaaacgg gccacttact ctcccagcca
     5881 ttcaacagct cgacccccgt actctattac aacaaagacg ccttcaagaa agcggggtta
     5941 gacccggaac aaccgccgaa aacctggcag gatctggcgg actatgccgc gaaactgaaa
     6001 gactccggta tgaagtgcgg ctacgccagc ggctggcagg gctggatcca actggaaaac
     6061 tttagcgcct ggaacggtct gccgtttgcc agcaaaaaca acggctttga cggcacggac
     6121 gcggtgctgg agttcaacaa gccggagcag gtgaaacaca tcgccatgct cgaggaaatg
     6181 aacaagaagg gcgacttcag ctacgtcggt cgtaaggatg aatccaccga gaagttctat
     6241 aacggtgatt gcgcgatgac caccgcctct tccggttctc ttgccaacat tcgcgagtac
     6301 gccaaattca actacggcgt aggcatgatg ccttacgacg ccgatgcgaa agatgcgcca
     6361 caaaacgcca ttatcggcgg agccagcctg tgggtgatgc agggtaaaga taaagaaacg
     6421 tataccggtg tggcgaagtt cctcgatttc ctcgcgaagc cagaaaacgc tgctgagtgg
     6481 catcagaaaa ccggttatct gccaattacc aaagcggcgt atgacctgac ccgtgagcag
     6541 ggcttttatg agaaaaaccc aggggcggat accgcgacac gtcagatgct gaacaagccg
     6601 ccgttgccgt tcaccaaagg gctgcgtctg ggcaacatgc cgcagatccg cgtgattgtg
     6661 gatgaagagc tggagagcgt gtggaccggt aagaagacac cgcagcaggc actggatacc
     6721 gccgttgagc gtgggaacca gttactgcgt cgctttgaga aatcgacgaa gtcttaatca
     6781 gtgtaatgtc ggatgcgttt cgcttatctg acctggcatc gcgtgtaggc cggataagcg
     6841 aagcgcatcc ggcacagttc aggaattaac cgtaatgtca tcatcccgtc cggtgttccg
     6901 ctcgcgctgg ctgccttatc tgctggtcgc gccgcagctc atcatcaccg ttatcttttt
     6961 tatctggcct gcgggcgaag cgttgtggta ctcgctacaa agcgtcgatc cgtttggttt
     7021 ctccagccag tttgtcggtc tggataactt cgtcacgctg tttcatgaca gctattatct
     7081 cgactccttc tggacgacga taaaattcag cacctttgtc accgtcagcg gtttgctggt
     7141 gtcgctgttc ttcgcggcgc tggtggagta catcgtgcgc ggcagccgtt tctatcaaac
     7201 cttaatgctg ctgccttacg ccgtggctcc cgccgttgcc gccgtattgt ggatcttcct
     7261 gtttaacccc ggtcgcgggc tgatcaccca ttttctcgcg gagttcggct acgactggaa
     7321 ccacgcgcaa aacagcggtc aggcaatgtt tctggtggtg tttgcctcag tatggaagca
     7381 aatcagctac aacttcctgt tcttctatgc cgcgctgcaa tccattcccc gttcgttgat
     7441 cgaagccgca gccatcgacg gtgcaggccc aattcgccgc ttctttaaga ttgcgctgcc
     7501 gcttatcgct ccggtgagtt tcttcctgct ggtggtgaac ctggtgtatg ccttcttcga
     7561 caccttcccg gtgatcgacg ccgccacgtc cggcgggccg gttcaggcca ccacgacgct
     7621 gatttataag atctaccgcg aaggttttac cggactggat ctggcttcgt ctgccgcaca
     7681 gtcggtggtg ttgatgttcc tcgtcatcgt gctgacggtg gtgcagttcc gctatgttga
     7741 aagcaaggtg cgttaccaat gattgagaac cgtccgtggc tgacgatatt cagccatacc
     7801 atgctgatcc tcgggatcgc ggtgatcctc ttcccgctgt acgtggcgtt tgtcgcggcg
     7861 acgctggata aacaggccgt ctatgccgcg ccgatgacgc tcatccccgg cacacatctg
     7921 ctggaaaaca tccacaacat ctgggtgaac ggggtaggca cgaatagcgc gccgttctgg
     7981 cggatgttgc ttaacagctt tgtgatggcg ttcagcatta cgctcggcaa aattaccgtc
     8041 tcgatgctct cggcatttgc cattgtctgg tttcgttttc cgctacgtaa cctcttcttc
     8101 tggatgattt ttatcaccct gatgctgccg gttgaagtac gtatcttccc gacggtggaa
     8161 gtcatcgcca acctgcagat gctcgacagc tacgccggtt taacgctgcc gctgatggcc
     8221 tcggcgaccg ctactttcct gttccgccag ttctttatga cgctgccgga tgagctggtg
     8281 gaagccgcgc ggatcgacgg cgcatcgcca atgcgcttct tttgcgacat cgtttttccg
     8341 ctctccaaaa ctaatctggc ggcgctgttt gtgatcacct ttatctacgg ctggaatcag
     8401 tatttgtggc cgttgttgat tattaccgac gttgatctcg gcaccaccgt ggcagggatc
     8461 aaagggatga tcgccactgg cgaaggcacc acggaatgga actcggtaat ggcggcgatg
     8521 ttgttaacgc ttatccctcc ggtggtgatt gttttagtga tgcagcgtgc tttcgtgcgc
     8581 ggcctggtcg atagtgagaa ataagatggc aggactgaaa ttacaggcag taaccaaaag
     8641 ctgggatggc aaaacccagg tgattaaacc actgaccctt gatgtggcgg atggcgaatt
     8701 tatcgtgatg gtcgggccgt ctggctgcgg aaaatcgacg ctgctgcgca tggttgccgg
     8761 gctggagcgg gtgacggaag gcgatatctg gatcaacgac cagcgcgtga ccgaaatgga
     8821 gccgaaagat cgcgggattg cgatggtgtt ccagaactac gcgctttatc cgcatatgag
     8881 tgtcgaagaa aacatggcgt gggggctgaa aattcgcggc atgggcaagc agcaaattgc
     8941 cgagcgcgtt aaagaagcgg cgcgcattct ggagctggac ggtctgctca aacgtcgccc
     9001 gcgcgagctt tccggcggtc agcgccagcg tgtggcgatg ggccgcgcga ttgtgcgcga
     9061 tccggcggtg ttcctgtttg atgagccgct ctctaacctc gatgccaagc tgcgcgtgca
     9121 gatgcgtctt gaactgcaac agttgcaccg tcgcctgaaa acgacttcac tctacgttac
     9181 tcacgatcag gttgaagcga tgacgctcgc ccagcgagta atggtgatga acggcggcgt
     9241 tgccgaacag attggcacac cagttgaagt ctacgaaaag cccgccagcc tgtttgtagc
     9301 gagttttatc ggcagtccgg cgatgaatct gctgacaggc cgcgtgaata acgaaggcac
     9361 gcatttcgaa ctggacggcg gtattgagct gccgctaaac ggtggctacc gtcagtatgc
     9421 cgggcgtaaa atgactctcg gcattcgccc ggaacatatt gcgctaagct cgcaggcaga
     9481 aggcggcgta ccgctggtga tggacacgct ggagatcctc ggcgcagata acctggcgca
     9541 cggacgctgg ggcgagcaga agctggtggt gcgactggcg catcaggagc gcccgacggc
     9601 aggcagcacg ctgtggctgc atctggcgga aaatcagctg catctttttg atggtgaaac
     9661 aggacaacga gtatgagtaa ctggccttat ccccgcatcg tcgctcatcg tggcggcggt
     9721 aagctggccc cggaaaacac cctggcggca atcgacgtcg gggcaaaata cggtcataag
     9781 atgatcgaat ttgacgcgaa gttatcgaaa gatggcgaga tcttcctgct ccatgacgac
     9841 aatctcgaac gcaccagcaa cggctggggc gtcgcgggtg aactgaactg gcaggattta
     9901 ctgcgcgtgg atgcgggcag ttggtacagc aaagcgttta aaggtgagcc gctgccgttg
     9961 ctttcgcagg tggcggaacg ctgtcgtgaa cacgggatga tggcgaatat cgaaatcaaa
    10021 cccaccactg gcactggacc attaacgggc aaaatggtgg cgctggcggc acgccaactg
    10081 tgggccggta tgacgccgcc gctgctgtca tcgtttgaga ttgatgcttt agaagccgca
    10141 caacaggcag taccggaact gccgcgcggt ttgttgctgg atgagtggcg cgacgactgg
    10201 cgcgaactga ccgcgcggct gggctgcgtc tctattcatc tcaatcataa gttactcgat
    10261 aaagtgcgag tgatgcagtt gaaagacgcc ggactgcgga ttctggttta taccgtcaat
    10321 aaaccccagc gcgcggcaga gttgctgcgc tggggcgtgg attgcatctg taccgatgcg
    10381 attgacgtga ttggtccgaa ctttacggcc caatagtttt caacggaatg tcaggctgcg
    10441 gcgtgctggg ctgattcagc atgtcgccgt ttctcctctc cggcagcata tgctgctgat
    10501 tcaacgaact atccggatta cggttgctgt ttaacatccc gccgttggtg ttgggcagca
    10561 tttgctgccg ggcgggattt cgctcccccg gctgtgactg caacacccgc tgagaattat
    10621 tgtttatctg gttttctaaa tgctgctgtt gcaactgagt ttgcgttttc agttgctgat
    10681 tcagcatccc tttttgctgg atttgctgag tctgcatctg ggtttgcatc cgctgctggc
    10741 tgggtatctg ataccccggc tggttagggt tgttcagagt attaatgggc tgtgcaaagc
    10801 cgacaaacgg caggagtgcc gtaagaatca gaagtcgttt catcgcgtat cctcctctga
    10861 agatatcctt taagtttact cgcttcccga caaaacgatg attaattcag agttatatac
    10921 caggcttagc tggggttgcc ccttaatctc tggagaataa cgatgataaa accgacgttt
    10981 ttacgccggg tggccattgc tgctctgctc tcaggaagtt gttttagcgc cgccgccgcg
    11041 cctcctgcgc cgcccgtctc gtatggtgtg gaggaagatg tcttccaccc ggtacgcgcg
    11101 aaacagggaa tggtagcgtc tgtggacgcc actgccactc aggtgggggt ggatatcctc
    11161 aaggagggcg ggaatgccgt tgatgccgcc gtggcggtgg gctacgcgct ggcggtaacg
    11221 catccgcagg cagggaatct gggcggtggt ggttttatgt taatccgctc gaaaaatggc
    11281 aataccacgg ctatcgattt ccgcgaaatg gcacccgcca aagcgacccg cgatatgttc
    11341 ctcgatgatc agggcaaccc ggacagcaaa aaatcactca cttcgcatct ggcttccggc
    11401 acaccgggta cggtagcagg tttctcgctg gcgctggata aatacggcac catgccgctg
    11461 aacaaagtcg tgcagcccgc gtttaaactg gcacgcgatg gttttatcgt taacgacgcg
    11521 ctggctgacg atctcaaaac ctacggtagc gaagtgttgc cgaatcacga aaacagtaaa
    11581 gctatcttct ggaaagaggg cgagccgctg aaaaagggcg acacgctggt gcaggcgaac
    11641 ctggcaaaga gcctggagat gattgctgaa aacggcccgg acgaattcta taaaggcacg
    11701 attgcggaac agatcgccca ggagatgcag aaaaacggtg gcttgatcac taaagaagat
    11761 ttagcggcct ataaagcggt cgaacgcact ccgataagcg gcgattatcg cgggtatcag
    11821 gtttactcca tgccaccgcc atcctccggc gggatccata tcgtacaaat cctcaatatt
    11881 ctggaaaact tcgatatgaa gaaatacggc tttggcagcg ccgatgcgat gcaaatcatg
    11941 gcagaagcgg agaaatacgc ctacgccgac cgctcggaat atcttggcga cccggatttt
    12001 gtcaaagtac cgtggcaggc gctgaccaat aaagcctatg ccaaatctat tgccgatcaa
    12061 attgatatca ataaagcgaa gccatccagc gaaattcgcc ccggcaagct tgcgccttat
    12121 gagagtaatc aaactaccca ttactcagtg gtggataaag atggtaacgc ggtggcggtg
    12181 acctatacgc tgaacaccac cttcggtacg ggcattgtcg cgggcgagag cggtattctg
    12241 cttaataatc agatggatga tttctccgct aaaccgggcg taccgaacgt ttacgggctg
    12301 gtgggcggtg atgccaacgc cgtcgggccg aataaacgtc cgctgtcgtc aatgtcacca
    12361 acgattgtgg tgaaagacgg taaaacctgg ctggttaccg gtagccctgg cgggagccgg
    12421 atcatcacta cagtgctgca aatggtggtg aatagcatcg attatggcat gaacgtcgcc
    12481 gaagcgacca atgcgccgcg tttccaccat cagtggttgc cggacgagct gcgtgtcgaa
    12541 aaagggttta gcccggatac gctcaagctg ctggaagcaa aaggtcagaa agtggcgctg
    12601 aaagaggcga tgggcagtac acaaagcatt atggttgggc cggacggtga gttgtacggc
    12661 gcatccgacc cgcgctcggt ggatgattta acggcggggt actaaggtta gcggccctct
    12721 tcgtgggaag agggctattt tgtcagggca agccgaaggt agcctttttt atttcgtaat
    12781 cctgtagata ttcttccagc ggctttgccg ttcccagaga atgaatttca ccactgtctt
    12841 tatcaataat aaaaggtgcg ttaccagcta agcgcgcggc ctcatctcca gtttcgagaa
    12901 attctcgtgc ttcgaaacag aaataccagc cctggctaaa gcgtccatgt agagtaatga
    12961 cgaccgggag atctgcatca tcaaggtaat ggttcgcttt cgcgaatgcg tcgtgataag
    13021 taatcataat tataataaat attattgttg agtgttatat tattattcct gtgtgataca
    13081 ttgagcaaag acgcgttcat cttcgtcaaa gat
//
LOCUS       DAJSKJ010000111        13024 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000111 DAJSKJ010000000
VERSION     DAJSKJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.111"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            381..1070
                     /gene="mutH"
                     /locus_tag="ON345_002682"
     CDS             381..1070
                     /gene="mutH"
                     /locus_tag="ON345_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR3072720.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1139..1852
                     /locus_tag="ON345_002683"
     CDS             1139..1852
                     /locus_tag="ON345_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR3072721.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            1990..2208
                     /gene="ygdR"
                     /locus_tag="ON345_002684"
     CDS             1990..2208
                     /gene="ygdR"
                     /locus_tag="ON345_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR3072722.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2316..3356
                     /locus_tag="ON345_002685"
     CDS             2316..3356
                     /locus_tag="ON345_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR3072723.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSKADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYNWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3388..4581)
                     /gene="lplT"
                     /locus_tag="ON345_002686"
     CDS             complement(3388..4581)
                     /gene="lplT"
                     /locus_tag="ON345_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR3072724.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4574..6733)
                     /gene="aas"
                     /locus_tag="ON345_002687"
     CDS             complement(4574..6733)
                     /gene="aas"
                     /locus_tag="ON345_002687"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR3072725.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENARGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALSVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7319..8350
                     /gene="galR"
                     /locus_tag="ON345_002688"
     CDS             7319..8350
                     /gene="galR"
                     /locus_tag="ON345_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR3072726.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGINFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8357..9619)
                     /gene="lysA"
                     /locus_tag="ON345_002689"
     CDS             complement(8357..9619)
                     /gene="lysA"
                     /locus_tag="ON345_002689"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR3072727.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAVLDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9741..10676
                     /gene="lysR"
                     /locus_tag="ON345_002690"
     CDS             9741..10676
                     /gene="lysR"
                     /locus_tag="ON345_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR3072728.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10663..11355)
                     /gene="ygeA"
                     /locus_tag="ON345_002691"
     CDS             complement(10663..11355)
                     /gene="ygeA"
                     /locus_tag="ON345_002691"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR3072729.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYSMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11484..12902)
                     /gene="araE"
                     /locus_tag="ON345_002692"
     CDS             complement(11484..12902)
                     /gene="araE"
                     /locus_tag="ON345_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR3072730.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGI"
BASE COUNT         3303 a         3498 c         3201 g         3022 t
ORIGIN      
        1 gtaattcgtg acccaggtca cacctctcat ttacgggttg gcgaaagatc aaccaatgcc
       61 gtatttatcc acagaatgtg ccactaagtt aagcactgaa ccactaaaaa ctggagtttc
      121 gtcgcacgtc aaggctgtaa atggaaacag tagtggaggt ttttcacagt tatcccagct
      181 ttctgtggat aacatggtgt aagatcctgt ttattttcag tgaccagatt tggaaaaccc
      241 gttgcagtgt tgcgcaactc gattaccggc aacctaaaaa gcaatataaa tcagagaatt
      301 gaacaacgca tgtggaaaaa gttacactgc gaatattcgg cacataattg ctgtttgttt
      361 tttaaccaag gtatcatgac atgtcccaac ctcgcccact gctctctcct cccgaaactg
      421 aagaacagtt gttagcgcaa gcacagcaac tttctggtta tacattggga gaactggcgg
      481 cacttgccgg gctggttacg ccagagaatt taaaacgcga taagggctgg attggcgtgt
      541 tactggagat ctggctaggt gccagcgcag ggagtaaacc tgagcaagat tttgctgctc
      601 tgggcgtgga acttaaaact atccctgtgg atagtcttgg tcgtccgctg gaaacaacat
      661 tcgtttgtgt tgccccgtta acgggtaata gcggggtgac ctgggaaacc agccacgtgc
      721 gccacaagct caaacgcgta ctgtggatac cggttgaagg cgagcgcagc atcccgctgg
      781 cgcagcgtcg cgtaggatcc ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc
      841 tacgcgaaga ctgggaagaa ttaatggata tgattgttct cggtcaggtt gagcggatca
      901 ccgctcgtca cggggaatat ttacagataa gaccgaaagc agcgaatgcg aaagcgctta
      961 ccgaagccat tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc ttttatttga
     1021 agaagaattt caccagtgca ctactggccc gtcattttct gatccagtag ccatcgcttt
     1081 gacctgccgc tttccgggca tataattacc gcttcatttt tttggcaggg ctttttagat
     1141 gttatttgca tggataaccg atcctaacgc ctggcttgcg ctcggtacgc tgacgctgct
     1201 ggagatcgtt cttgggatcg acaatattat tttcctttct ctggtggtgg caaagcttcc
     1261 cactgcacaa cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct
     1321 ggcgctgctg gcatcaatcg cctgggttac gcgcctgaca aatccgcttt ttacaatatt
     1381 cagtcaggaa atttctgccc gtgatttgat tctgcttctg ggtggcttgt tccttatctg
     1441 gaaagccagc aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg
     1501 cgtttcatca ttcctcggcg ctatcgtgca gattatgctg ctggatatta tctttagcct
     1561 cgactcggtg attacggctg tgggtctgtc agatcacctg tttattatga tggcagccgt
     1621 ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg
     1681 ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct ttaccctgat
     1741 tctggaaagt ttcgacatcc acgtaccgaa aggttacatc tacttcgcga tgttcttctc
     1801 tattgcggtt gaaagcctca acttgattcg caacaaaaag aatccgctct gatactccgt
     1861 acgctctcct gcgggagggc gtactcactc cttttaccct cacagtttaa gattttcctg
     1921 ctttcaagat atataacgtc ggtttataaa cagactatta tcataggtag gccaaatgag
     1981 gataaccgga tgaaaaaatg ggcagtaata atttctgcag tcggactggc gtttgctgtt
     2041 tccgggtgtt ccagtgatta cgtcatggcg accaaagatg gccgtatgat tttgaccgat
     2101 ggaaaacctg aaattgatga tgataccggg ctggtgagtt atcacgatca gcaaggtaac
     2161 gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca
     2221 gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat
     2281 attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca
     2341 gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagca
     2401 aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg
     2461 ttgccgaaat gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg
     2521 tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga
     2581 gcggaccgtc gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga
     2641 atatccgcga agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt
     2701 atcaggtgca ctggccgcag cgcccgacca actgcttcgg caaactcggt tataactgga
     2761 cggattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac
     2821 gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct
     2881 acctgcatct ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca
     2941 gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg
     3001 aactgctggc ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg
     3061 caaaacccgc tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc
     3121 aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatggc ctggaccctg
     3181 ctcagatggc gctcgcgttt gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg
     3241 caaccacgat ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag
     3301 acgtattagc tgaaattgaa gcggtgcatc aggtttatac ttatccggca ccataagatt
     3361 aacgccggtt aaaaccggcg ttaaatatta atgacggcgc tgccagatcc acagcgccgt
     3421 tattgccagc gcaaacagcg caccgaagcc aatgccaatg ggcacgaccg ggatgcctat
     3481 cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt cgccaaggtt
     3541 ttgtactgca atcgcattcc ccgccccgac gcttttttta ccccgctcct gtagcaacgc
     3601 attgagcgga acgacaaaaa agccccccat cacgccaatc agcatcaaca aggcataggc
     3661 tggcagcagc tcgtgttgca gggaaaaaat cagtaccacc acgccaatca aaatcccggc
     3721 tggcatacag cgtgacacgg tttccagcgt aactaacttc gctgccgcac ctgcgccaac
     3781 cacgatacca atcgctacca tcgcgttgag ataggtgggc gtagcgttat cggtaatgcc
     3841 cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacccg ctccccagaa
     3901 taaactggtg cccaccagcg aaaaacgcgt ttcaccattg cgccatagcg aggtgcaggc
     3961 attcaggaaa ctgcgggtca tgttgatgag attccaggac tgccccggac gcgccgccgc
     4021 cagtttggga atgtagatat tggcaacgac cgcaccaccg taggccagtg cgcatgcggc
     4081 cagggcgacg aggacatgcc agtcagccag cacaccaccg gctacggaac cgagcaaaat
     4141 cgccgctatg gtagaagctt ccattaaacc gttagctttc actaacttac tacccgtggt
     4201 taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa cacccaccag
     4261 cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca gcagcttcag
     4321 gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct gcccgacaaa
     4381 cggcgcaaaa agaatgtaag cacctacaaa caccatttgc aggatgggct ggctccactc
     4441 cggatagaac tgcgctttca gtaacgccag agtggcaaac agtagggcat tatcgccaaa
     4501 cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt
     4561 agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga
     4621 caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga atatcgcgcg
     4681 gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca
     4741 gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg ctcttaatcg
     4801 cagtggcatg gactttatct ggcgaaacac taagtgccag ttgttccacc atttccagcg
     4861 acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc
     4921 cctgctcgtc aaaacgcaca atatcgccag tgtcatacca gccgcgctcc atttcgccgc
     4981 gagcattctc ggcggtgggc acttccagta cacctggctt ctccacccgc agatagccgt
     5041 tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacca ggaaccgaca
     5101 acaggcgcgc atccattcct ggtagaatac gccctaccgt accgggtttc gccgccatcg
     5161 gtacgttgat agaaacgaca ggcgcgcact cggttacacc atagccttca aggatgcgca
     5221 ggccaaattt atcctgccaa agctgtttgg tactttcttg tagtttttct gcgcctgcca
     5281 ccacatagcg tagacgatag aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga
     5341 aagtcgaggt accgaacaac acggtgcaac tgcggtcata caccaactcc ggcacaatgc
     5401 ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtaagcagt ggcgtaaaca
     5461 ggcctaccgt cagcccaaag gagtgaaaca gcggtaatgc cgacataaag cgatcgttgg
     5521 tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt ttatggctat
     5581 ggacgacgcc ttttggatgg ccttcagaac cggaggtaaa gaggatcagc gcctcttctt
     5641 ccggctgctg ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag atccatactt
     5701 tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc acctgagtaa
     5761 gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg
     5821 ttttgatttc agccgccgta atagcactgg tcagcccttt taccccagca gtgtagttca
     5881 tcattgcggg aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca ctgatgcctg
     5941 cattgggcag cattaagccg atgcgttcgc cttcaacact gtatttttca aggatgcgtc
     6001 caacaaacag cgttttcgta agcaatttgc gataggagtc tggggtaaag ttgacgtctt
     6061 cgacacattt cttcccggct ccgaagcggt acattgcact cagtaaagat tcgtacagcg
     6121 tttcacgcgg gcgcaccgcc attcgcgctt ccatcattat ttgatgcagc atttcgccag
     6181 cgattttgcg acggtcacgg gcacgcggcg catccggcat cgccacctgc gttggtggca
     6241 aaatatgcag agtaatttgc gggaacaagc gacgtttaac cagacctttc aggcggctga
     6301 agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca gacttcgccg
     6361 cgacaaaacc cgcgccatcg tagattttca tcagcgagcc tgtagtggtg atgcgtcctt
     6421 cagggaaaat caccactggt cggccctgtt ccaccagacg taccagatgt ttaatagcca
     6481 taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa cgcatatacc
     6541 actgttggct tattgaggtg taaacggcaa acactggacg cacaggtaaa aacagtccaa
     6601 gcaaaatgcc atcaataaaa gagacgtgat taggcgtaat tagaacgcgc tcgcccttca
     6661 gtgcctgggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa
     6721 agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg gagggagatt
     6781 acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg
     6841 cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg aataatctca ccaatcaata
     6901 cctctgggat cttgattgtg gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg
     6961 aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg
     7021 gccaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca acccgaagtt
     7081 gacttcacct atcaatacct ctgggatcac cactttagca acctgaagcc aaacgccacc
     7141 agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt
     7201 gtcttactta tttgcgtttg gctcacattc ccacgatgaa aacacgccac cccttgaacc
     7261 aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt acccactaag gtattttcat
     7321 ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt
     7381 cattaataat tcacctaaag ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga
     7441 gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac
     7501 ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt
     7561 cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt accacaacga
     7621 acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt
     7681 ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa tgcccggtat
     7741 ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg
     7801 ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gtattggtta
     7861 tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc
     7921 ccttgctgaa agtggtattg cggccaatga ccggctggtg acatttggcg aaccagacga
     7981 aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggaataaatt tcactgcggt
     8041 agcctgttat aacgattcaa tggcggcggg tgcgatgggc gttctcaatg ataatggtat
     8101 tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt
     8161 gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg atggcgaccc aggctgccga
     8221 actggctttg gcgctggcgg ataatcgccc tctcccggaa attactaatg tatttagtcc
     8281 gacgctggta cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac
     8341 cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg gtctggcgac
     8401 ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc ggacggctat
     8461 tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc agataatcac
     8521 ctgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga
     8581 catcgcccga ttcacataac ggtccggcga cgacggtttc caccgttggc gcgtgttcca
     8641 gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac attgccgggc
     8701 gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctcccc atttgtttga
     8761 cgctccgcac ctgagtaatt aatacgccag actgcgctac caggaagcga cccggttcaa
     8821 tttccagttt cacagggtgg cccaaatggc gggcgatttg ctcacgcgcg gcattccaca
     8881 gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga acagaaagcc
     8941 caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gatgacctga cgcaccatag
     9001 caccacacac ctgttccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc
     9061 cgaccagctg cagatgatga cgttgtatca cgtccagtac ggcgggcaga tcggtgtacc
     9121 agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa
     9181 accccggatt aacgcgcagc catacccgat gccctggcga aacctggccc agttggtcga
     9241 gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg
     9301 cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac
     9361 ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct
     9421 cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag cgcaccacat
     9481 caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg
     9541 ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg gtatcggtgc
     9601 tgaacagtga atgtggcata acaaactcca gataagtgct tttttatgat tacgccacat
     9661 cataaaaaga ataaaaaata tcgatttatg tcgagtatat gcaaaaatga tatggattac
     9721 cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg
     9781 cggtaatgac cgccggaagc ctgactgagg cggcacacct gctacacacc tcacagccaa
     9841 ccgtcagccg cgaacttgcg cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg
     9901 tacgtgggcg attacatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat
     9961 cctggtacgg actggatcga attgtcagcg ctgcagaaag tctgcgcgag tttcgccagg
    10021 gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc
    10081 aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc
    10141 tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag acactccata
    10201 cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc
    10261 cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag ggtgagaact
    10321 acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta tttactgaac
    10381 atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg
    10441 tacgggcggg ggtaggtgtt tcggtggtta acccgctcac cgcactggat tatgcggcaa
    10501 gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc
    10561 ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag tgagcattta caagcggggt
    10621 taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca gcataaaagc
    10681 gacagcatcc tcggcatgga tcgccgcagt atcaaacaca ggcaaaacac tgcgctcttc
    10741 tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc
    10801 aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt gccccagaca
    10861 cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag gaataagaca
    10921 attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggagtaacg
    10981 cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc cggtggcatc
    11041 cgcaatgtgt aagaaaggca gagtgcaacg tgactcaatg gcatccgcca ctttatgcat
    11101 cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc
    11161 agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt ctatttcatg
    11221 aaaatcgacg ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc gctgtttaat
    11281 gccttcattt atcaaacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa
    11341 accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa
    11401 cgagacaaac gcctcaacgg ccaagtgccc gatctctatt aacgaaaaaa gggccggatg
    11461 tacagcacat ccggcccgtg aaatcagatg ccgatatttc tcaacttctc gcctgccatc
    11521 agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagtg
    11581 atgcccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc agcgccaatg
    11641 ctgtcgagca gcgtcaggaa ggtcgcgccg ataatcatat tcgacaccca gttcgtggtg
    11701 gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga gcacaggatc
    11761 cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca
    11821 gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag gcaatagccc
    11881 agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgccct
    11941 gctttatcta ccgtaaacac cgcaataaag gtggcgaaca taaaggtcag ccctacgacc
    12001 agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc
    12061 gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg
    12121 agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc gccctgtttt
    12181 aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc
    12241 agcatacgca atacttcttc cgcctcaata tgacgcccct tttccgccag ccagcgcggg
    12301 ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc aagaaccccc
    12361 aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg
    12421 atgccgagtg tgaccatcaa ctggtacata ctgatcatct taccgcgaac gttttcactt
    12481 gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc
    12541 accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc tatagaaccg
    12601 agtacaaaca ggatggcccc cgccatcagg ctgtatttac gccccaggcg gaacgacagc
    12661 caaccattaa acagcgcacc aattgctgca ccgagcatca tgctactaac cacccattcc
    12721 tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg
    12781 ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc
    12841 atacgccgcg tatcccgcaa agaacgtggc gttaaagcag attccgtatt gatagtaacc
    12901 attttttcct gccagcagag agtaagacat agtgaaaaaa tacgtgaaca actcacacag
    12961 gtgtcaggtc ggaaacagca taaatatgga ttaaattgct acgacatgtc gttatgtgat
    13021 ggat
//
LOCUS       DAJSKJ010000112        13024 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000112 DAJSKJ010000000
VERSION     DAJSKJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.112"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..666
                     /locus_tag="ON345_002693"
     CDS             <1..666
                     /locus_tag="ON345_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR3072731.1"
                     /translation="SQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKWDKD
                     NGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNITLGGGNGGTTRISN
                     VSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVELDNKLSKTESKLSGGIASAMAMTGL
                     PQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAG
                     IQW"
     gene            1034..2689
                     /gene="lldP"
                     /locus_tag="ON345_002694"
     CDS             1034..2689
                     /gene="lldP"
                     /locus_tag="ON345_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR3072732.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2689..3465
                     /gene="lldR"
                     /locus_tag="ON345_002695"
     CDS             2689..3465
                     /gene="lldR"
                     /locus_tag="ON345_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR3072733.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3462..4652
                     /gene="lldD"
                     /locus_tag="ON345_002696"
     CDS             3462..4652
                     /gene="lldD"
                     /locus_tag="ON345_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR3072734.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            4838..5311
                     /gene="trmL"
                     /locus_tag="ON345_002697"
     CDS             4838..5311
                     /gene="trmL"
                     /locus_tag="ON345_002697"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR3072735.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(5364..6185)
                     /gene="cysE"
                     /locus_tag="ON345_002698"
     CDS             complement(5364..6185)
                     /gene="cysE"
                     /locus_tag="ON345_002698"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR3072736.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(6265..7284)
                     /gene="gpsA"
                     /locus_tag="ON345_002699"
     CDS             complement(6265..7284)
                     /gene="gpsA"
                     /locus_tag="ON345_002699"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3072737.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(7284..7751)
                     /gene="secB"
                     /locus_tag="ON345_002700"
     CDS             complement(7284..7751)
                     /gene="secB"
                     /locus_tag="ON345_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR3072738.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(7814..8065)
                     /gene="grxC"
                     /locus_tag="ON345_002701"
     CDS             complement(7814..8065)
                     /gene="grxC"
                     /locus_tag="ON345_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR3072739.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(8207..8638)
                     /locus_tag="ON345_002702"
     CDS             complement(8207..8638)
                     /locus_tag="ON345_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3072740.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            8883..10427
                     /gene="gpmM"
                     /locus_tag="ON345_002703"
                     /gene_synonym="pgmI"
     CDS             8883..10427
                     /gene="gpmM"
                     /locus_tag="ON345_002703"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR3072741.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            10437..11720
                     /gene="envC"
                     /locus_tag="ON345_002704"
     CDS             10437..11720
                     /gene="envC"
                     /locus_tag="ON345_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR3072742.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            11724..12683
                     /locus_tag="ON345_002705"
     CDS             11724..12683
                     /locus_tag="ON345_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR3072743.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(12670..>13024)
                     /gene="waaH"
                     /locus_tag="ON345_002706"
     CDS             complement(12670..>13024)
                     /gene="waaH"
                     /locus_tag="ON345_002706"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR3072744.1"
                     /translation="QGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEA
                     LRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDK
                     TRSHHRITRSAFNLR"
BASE COUNT         3075 a         3494 c         3468 g         2987 t
ORIGIN      
        1 tctcagctga aaaccaccaa cgatgccgtg gcgacgaata ccaccaatat cgccactaac
       61 accaccaaca tctctaatct gactgagacg gtgactaatc ttggtgagga tgcgctgaaa
      121 tgggataagg acaatggtgt cttcactgca gctcatggca acaataccgc cagcaaaatc
      181 accaatatcc tggacggcac agtcactgca accagttccg atgccattaa cggtagccag
      241 ctttatgact taagcagcaa tatcaccctc ggtggcggca acggcggtac gactcgtatc
      301 agcaacgtct ccgctggcgt caacaacaac gacgcggtga attacgcgca gttgaagcaa
      361 agcgtgcagg aaacgaagca atacaccgat cagcgaatgg ttgagttgga taacaaactg
      421 tctaaaactg aaagcaagtt gagcggtggt atcgcttctg caatggcaat gaccggtctg
      481 ccgcaggctt acactccagg tgccagcatg gcctctattg gtggcggtac ttacaacggt
      541 gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa
      601 ttacaaggta gtaccaatag ccagggtgaa tactccgccg cactcggtgc cggtattcag
      661 tggtaatgat ccattaagtt agtgtgacta agggcaaacg ccaaattgcc tgatgcgtta
      721 cgcgcatcag gcaagataag gcgctccggg tcaacaatct aagggcaatt ctctgatgag
      781 gattgccctt ttctttacca gacatctccc cccacaagaa ttggccctac caattcttcg
      841 cttatctgac ctctggttca caattcccca attaaaactc acatcaatat tgccaataca
      901 taacatttag ttaaccattc attgtcatta tccctacaca acacaattgg cagtgccact
      961 tttacacaac gtgtgacaag gagatgagca acagactcat tacacgatgt gcgtggactc
     1021 caggagacct gcaatgaatc tctggcaaca aaactacgat cccgccggga atatctggct
     1081 ttccagtctg atagcatcgc ttcccatcct gtttttcttc tttgcgctga ttaagctcaa
     1141 actgaaagga tacgtcgccg cctcatggac ggtagcaatc gcccttgccg tggctttgct
     1201 gttctataaa atgccggtcg ctaacgcgct ggcctcggtg gtttatggct tcttctatgg
     1261 tttgtggccc atcgcgtgga tcattattgc agcagtgttc gtctataaga tctcggtgaa
     1321 aaccgggcag tttgacatca ttcgctcgtc tattctttcg ataacccctg accagcgtct
     1381 gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt gaaggagccg caggctttgg
     1441 cgcaccggta gcaattaccg ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc
     1501 cgccggactg tgcctgattg ttaacaccgc gccagtggca tttggcgcga tgggcattcc
     1561 aatcctggtt gccggacaag taacaggcat cgacagtttt gagattggcc agatggtggg
     1621 gcgtcagcta ccgtttatga ccattatcgt gctgttctgg atcatggcga ttatggacgg
     1681 ctggcgcggt atcaaagaga cgtggcctgc ggtcgtggtt gcgggcggct cgtttgccat
     1741 cgctcagtac cttagctcca acttcattgg gccggagctg ccggacatta tctcttcgct
     1801 ggtatcactg ctctgcctga cgctgttcct caaacgctgg caaccagttc gcgtattccg
     1861 cttcggtgac ttgggggcgt cacaggttga tatgacgctg gcccacaccg gttacaccgc
     1921 gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg accgctaccg taacactgtg
     1981 gagcatcccg ccgtttaaag ccctgttcgc atcgggtggt gcgctgtatg agtgggtgat
     2041 caacattccg gtgccgtacc tcgataaact ggttgcccgt atgccaccag tggtcagcga
     2101 ggctacagcc tatgccgccg tgtttaagtt tgactggttc tctgccaccg gcaccgccat
     2161 tctgtttgct gcactgctct cgattgtctg gctgaagatg aaaccgtctg acgctatcag
     2221 caccttcggc agcacgctga aagaactggc tctacccatc tactccatcg gcatggtgct
     2281 ggcgttcgcc tttatctcga actattccgg actgtcatca acactggcac tggcactggc
     2341 gcacaccggt catgcattca ccttcttctc gccgttcctc ggctggctgg gggtattcct
     2401 gaccgggtcg gatacctcat ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca
     2461 acaaattggc gtctctgatc tgttgctggt tgccgccaat accaccggtg gcgtcaccgg
     2521 taagatgatc tccccgcaat ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa
     2581 agagtctgat ttgttccgct ttactgtcaa acacagcctg atcttcacct gtatggtggg
     2641 cgtgatcacc acgcttcagg cttatgtctt aacgtggatg attccttaat gattgtttta
     2701 cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac
     2761 ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg cgatgcaact cggcgtatca
     2821 cgtaattcac tacgcgaggc gctagcaaaa ctggtgagcg aaggcgtgct gctcagtcga
     2881 cgcggcggcg ggacgtttat tcgctggcgt catgacacat ggtcggagca aaacatcgtc
     2941 cagccgctaa aaacgctgat ggccgatgat ccggattaca gtttcgatat tctggaagcc
     3001 cgctacgcca ttgaagccag caccgcatgg catgcggcaa tgcgcgccac acctggcgac
     3061 aaagaaaaga ttcagctttg ctttgaagca acgctaagtg aagacccgga tatcgcctca
     3121 caagcggacg ttcgttttca tctggcgatt gccgaagcct cacataacat cgtgctgctg
     3181 caaaccatgc gcggtttctt cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg
     3241 atgtatctgg tgccaccggt tttttcacaa ctgaccgaac aacatcaggc tgtcattgac
     3301 gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt
     3361 gttcacacca ccatgaaacg attcgatgaa gatcaggctc gccacgcacg gattacccgc
     3421 ctgcccggtg agcataatga gcattcgagg gagaaaaacg catgattatt tccgcagcca
     3481 gcgattatcg cgccgcagcg caacgcattc tgccgccgtt cctgttccac tatatggatg
     3541 gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga agatttgtca gaagtggcac
     3601 tgcgccagcg tattctgaaa aacatgtctg acttaagcct ggaaacgacg ctgtttaatg
     3661 agaaattgtc gatgccggta gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc
     3721 gtggcgaagt tcaggcagcc aaagcggcag acgcgcatgg cattccgttt actctctcga
     3781 cggtttccgt ttgcccgatt gaagaagtcg cgccagccat caagcgccca atgtggttcc
     3841 agctttatgt actgcgcgat cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag
     3901 cgggttgttc gacgctggtt ttcaccgtgg atatgccgac accgggagcg cgttatcgtg
     3961 atgcgcattc tgggatgagc ggcccaaatg cggcaatgcg ccgctacttg caagcggtga
     4021 cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg tccgcatgat ttaggtaata
     4081 tttcggctta cctcggcaaa ccgaccggac tggaagatta catcggctgg ctggggaata
     4141 acttcgatcc gtccatctca tggaaagacc ttgagtggat ccgcgatttc tgggatggcc
     4201 cgatggtgat caaagggatc ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg
     4261 ctgatgggat tgtagtttct aaccacggtg gccgccagct ggacggtgta ctctcttccg
     4321 ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga tatcgccatt ctggcggata
     4381 gcggaattcg taacgggctt gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac
     4441 tgctgggtcg tgctttcctg tatgcgctgg caacagcggg ccaggcgggt gtagctaacc
     4501 tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac gctgactggc gcgaaatcga
     4561 tcagtgaaat tacgcaagat tcgctggtgc aggggctggg taaagagttg cctacggcac
     4621 tggctccgat ggcgaaaggg aatgcggcat agtcgtttgc gccccctcac cctaaccctc
     4681 tccctcaggg agaggggacc gttcggcgct gtatgtactc cctcactctg aaacgacacc
     4741 gcactctttt tttctccctc gcccctccgg ggtgagggga aaaggccgca ctgctcacca
     4801 attttctgct atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga
     4861 accagaaatt ccgccaaata ctggcaatat catccgtctt tgcgctaata ccggctttcg
     4921 tctgcatatc atcgaaccga tgggatttgc ctgggacgat aagcgcctgc gccgcgcggg
     4981 gctggactat cacgagttta ccgccgttac gcgtcatcat gactttcgcg cgttcctcga
     5041 agcagaaaat ccccagcgcc tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc
     5101 cgtaaactat caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc
     5161 gagcattctt gatgccctgc ccgctgaaca aaaaattcgc attccgatgg tgccggacag
     5221 ccgcagcatg aacctgtcca atgcggtgtc agttgtggtg tatgaagcct ggcggcagct
     5281 gggatatccg ggagcgttgt tgagagatta gttactgtat gccggataga tgattacatc
     5341 gcatccggca cgatcacagg acatcagatc ccatccccat actcaaatgt atggttaata
     5401 ccgttgaaat gctggtccat atccattgat ggcttatcgc tgtctggttt accgacaata
     5461 cgagccggaa cgccagcggc ggttgtatgc ggcggcaccg gttgcagcac cacggaacct
     5521 gcgccaatct tcgcgccgcg accaacttca atattgccga ggattttcgc gcccgcgcca
     5581 atcatcacac cttcacgaat tttcgggtga cggtcaccac cagatttacc cgtaccgcca
     5641 agcgtcacag attgcagaat cgatacgtcg ttttcaatca ccgccgtttc acccacaacg
     5701 atgcccgtgg cgtggtcaag catgataccg cgaccaattt ttgctgccgg gtgaatatca
     5761 acctggaacg tcacagaaac ctggttttgc agaaagattg ccagtgcgcg acgtccctga
     5821 ttccacaacc agtgaccgat gcgatacgcc tgcaaggcat gaaaaccctt caggtataac
     5881 aacggggttg agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag
     5941 gccgcagagg cgatcatttc cgggtcagcg gcgtaggctt cttccacaac ttcacggata
     6001 gcaatagcag gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagtgcacta
     6061 ccaaggtttt cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag
     6121 tccgccagcg ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac
     6181 gacattgctt actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat
     6241 agcggtaaca aaggttccct ggggttagtg gctgctgcgc tcgtccttgc gtgcacgacc
     6301 taataacgtc aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc
     6361 ctcggttatt ggcatttcaa cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt
     6421 gcggtagcct tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg
     6481 accgagcatc atgccaaaac ggcggttacg cgactggtta tcggtacagg taagcaccag
     6541 atcccccagc cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcgccaag
     6601 acgcgacatt tcagccagcc cacgggtgat cagtgccgta cgcgcattcg caccaaaacc
     6661 gataccgtcg gacatccccg cgccaatggc aatgacgttt ttcaccgcac cgccaagctg
     6721 cacaccgatg aaatccgggt tgctgtaaac gcggaaactt ttgccacagt gcagcaattg
     6781 ttggagatca tcggcaaagg tctggtcggt agaggccaac gaaatagccg tcggtaaacc
     6841 tgccgccagt tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc
     6901 taaggcctca cgcgccacgt cctgtaacag acgtccggtt tccgcttcca gccctttggt
     6961 cgcccacacc agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc
     7021 aaagacatgg ctgggtacga cgacgagaat attacggctg gctgccagcg cagtggcgag
     7081 atcgctttca agatggagcg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca
     7141 gcggtcgcgt tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg
     7201 gccatttctt gccagggtaa tggcaagagc ggtgccgtac gagccggcac cgatcacagt
     7261 cattgaagca ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc
     7321 tgctgctgca aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc
     7381 gggaatgtac cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg
     7441 ttcgggcagt atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag
     7501 aaaataccgc cctgctgaac ttcacacagg aacgcggttt cttcgcccag ggaggccgtt
     7561 acggtaacac gcagtaccac ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc
     7621 agatcaagtt taacttctgg ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct
     7681 tcgaaagaga tatccttggt ataaatacgt tggatctgga aagtcatttc agtgttgttt
     7741 tgttctgaca tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatgc
     7801 caacgttcac acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca
     7861 agtcatcaca gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcgac
     7921 cgctgcgttt gatcatctct tcacgcttgg cggcattgcc atcgatcggc aactcctgaa
     7981 aactcacgcc cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt
     8041 tggtatagat ttcaacattg gccataattt agctcctgtt tttttaccct gaaatttcat
     8101 gttgcagggc ggctgcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg
     8161 gcctaattgc agctaacggc ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca
     8221 ccagaggcaa gttttcgcca gcccagccag caacgccctc tttcagcacg aatacttgcg
     8281 caaaaccagc tttcgtcagc gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta
     8341 ccacgataac cggtttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt
     8401 cactcggcaa cagattgata gaacctgcga tatggccttt acggaagtca tcacgctgac
     8461 gtaaatccac aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa
     8521 tcaccttcac tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg
     8581 cgatccaggc gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg
     8641 ggggtaacaa ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt
     8701 gcggcaaata cagccagcgc gttaactgga atgcaatttt gcggggcacg acgaaaaaaa
     8761 agcgcaaatc tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat
     8821 tttatcgatt cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg
     8881 caatgtcggt ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgtg
     8941 aagaacagca ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg
     9001 ccaatcgtcc gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc
     9061 agatgggtaa ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg
     9121 acctgactcg tctggacgtt gaaatcaaag atcgcgcttt ctttgctaat ccagtgctga
     9181 ctggcgcggt agataaagcg aaaaacgcag gtaaagcagt acacattatg ggtctgctct
     9241 ccgcaggcgg cgtacacagc cacgaagatc acatcatggc gatggtagaa ctggcagctg
     9301 aacgcggcgc agaaaaaatc tacctgcacg cattccttga cggtcgcgac accccgccgc
     9361 gcagtgctga atcctcgctg aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc
     9421 gcgtagcgtc catcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg
     9481 ttgaaaaagc ctatgacctg ctgactctgg cgcagggtga gttccaggcc gatactgccg
     9541 ttgctggttt gcaggcagct tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg
     9601 ttattcgtgc agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca
     9661 tgaacttccg tgctgaccgc gcgcgcgaaa tcactcgtgc attcgtaaac gcagatttcg
     9721 acggcttcgc gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg
     9781 ctgacatcaa aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt
     9841 ggatggcgaa aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg
     9901 ttactttctt cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga
     9961 tcaactcacc gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga
    10021 ccgaaaaact ggttgcggcc atcaagagcg gcaaatacga caccattatc tgtaactatc
    10081 cgaacggcga catggtaggt cacaccgggg tgatggaagc ggcggttaaa gcggttgaag
    10141 cactggatca ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt ggacaactgc
    10201 tgatcaccgc tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcac
    10261 acacggcaca caccaacctg ccagttccgc tgatttacgt tggtgataag aacgtgaaag
    10321 cggttgcagg cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg
    10381 aaatcccgca agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga
    10441 ggggaaaggc gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc
    10501 ccatcatcta cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg
    10561 cggatgagcg tgaccaactc aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg
    10621 tacgccaaaa gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag
    10681 cgatctctga agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata
    10741 aacagattga tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg
    10801 agcgcagcct cgccgcgcaa ctggatgccg cattccgtca gggcgagcat accggtattc
    10861 agctaattct cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct
    10921 atctcaacca ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtcg
    10981 ccatgcagcg tgctgaactg gaagagaaac aaagcgagca acaaacgctg ttatatgaac
    11041 agcgcgccca acaggcgaaa ctgactcagg ctctgaatga gcgtaaaaag acgctggcgg
    11101 ggctggaatc ttccatccag caaggtcagc agcagctaag tgaactgcgt gccaacgaat
    11161 cccgtctgcg taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gcagaacgag
    11221 aagcacgtga ggcccaggcg gttcgcgacc gccagaaaga agcgacgcgc aaaggcacca
    11281 cctacaaacc gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc ctgggtgcgc
    11341 cgcgtggtca ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggcgagcagc
    11401 tacagggtga attacgctgg aaagggatgg tgattggtgc ctctgaaggt actgaagtta
    11461 aagcgattgc cgacggtcgg gtgattctgg ctgactggct gcaaggctac ggtctggtgg
    11521 tggtggttga gcatggtaaa ggcgacatga gtctttacgg ctataatcag agcgcactgg
    11581 tgagcgttgg ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg
    11641 gtcagggtcg gccttcactc tatttcgaaa ttcgccgcca gggtcaggcg gtcaatccac
    11701 agccgtggtt gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg
    11761 ctctgttggc gctctcctcc cccgtacttg ctggcaaact tgccatcgtc attgatgatt
    11821 ttgggtatcg cccgcacaac gaaaaccagg tgctggcgat gccctccgct atctccgtcg
    11881 ctgtattacc cgattcaccg cacgccagag agatggcgac caaagcgcat aacagcgggc
    11941 acgaagtgtt gattcatctc ccgatggcac cactgagtaa acagccgctg gagaaaaata
    12001 cgctgcgccc ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata
    12061 acgtgcccta tgccgtgggg atcaacaacc acatgggtag caagatgacc tctaacctgt
    12121 ttggtatgca gaaagtgatg caggcactgg agcgttacaa tctttacttc ctcgacagcg
    12181 taaccatcgg taatacccag gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca
    12241 aacggaaggt gttcctcgac gattcgcaga acgaagcgga catccgtgtg caatttaatc
    12301 gcgcaattga tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt
    12361 caacggtacg cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga
    12421 aagccagcag cttgctgaat gaaccgcagg ttgatacgtc tacaccgccg aaaaacactg
    12481 tgcccgacac accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga
    12541 tagaacctgt ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca
    12601 cgctgatcgt ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcga
    12661 agtttaacgc tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt
    12721 gtcgcgccat tgccataagc ggaaagacca cagtaacgcc tggtaaccga ccttcacgct
    12781 gcgcacattg gtaatcaggc gcttatacat accggaagta aatatctcgg caatcatccg
    12841 ttggcgggta agaatatccg gctctttgcg caccgcatgg caaacacgca atgcttcgta
    12901 agttatttgc tgatgaaatt ccggataaat cgtaattttg tcggcataat ttcgatttaa
    12961 tttctccagc aggcgggtaa tcttaatata gtgacgttga taattaaggt ttttattccc
    13021 ttgt
//
LOCUS       DAJSKJ010000113        12989 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000113 DAJSKJ010000000
VERSION     DAJSKJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.113"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..384)
                     /locus_tag="ON345_002707"
     CDS             complement(<1..384)
                     /locus_tag="ON345_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HCR3072745.1"
                     /translation="MITHFRQAIEKTLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEG"
     gene            complement(406..1455)
                     /gene="allD"
                     /locus_tag="ON345_002708"
     CDS             complement(406..1455)
                     /gene="allD"
                     /locus_tag="ON345_002708"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HCR3072746.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATMAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGIAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1772..3439
                     /locus_tag="ON345_002709"
     CDS             1772..3439
                     /locus_tag="ON345_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCR3072747.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEVAGLLAGYLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINSGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            3449..4708
                     /locus_tag="ON345_002710"
     CDS             3449..4708
                     /locus_tag="ON345_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR3072748.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            4719..5534
                     /locus_tag="ON345_002711"
     CDS             4719..5534
                     /locus_tag="ON345_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR3072749.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            5531..6424
                     /locus_tag="ON345_002712"
     CDS             5531..6424
                     /locus_tag="ON345_002712"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR3072750.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEDTLAGEAGTCISL"
     gene            complement(6561..7628)
                     /gene="purK"
                     /locus_tag="ON345_002713"
     CDS             complement(6561..7628)
                     /gene="purK"
                     /locus_tag="ON345_002713"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR3072751.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(7625..8134)
                     /gene="purE"
                     /locus_tag="ON345_002714"
     CDS             complement(7625..8134)
                     /gene="purE"
                     /locus_tag="ON345_002714"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR3072752.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(8252..8974)
                     /gene="lpxH"
                     /locus_tag="ON345_002715"
     CDS             complement(8252..8974)
                     /gene="lpxH"
                     /locus_tag="ON345_002715"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR3072753.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKYQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(8977..9471)
                     /gene="ppiB"
                     /locus_tag="ON345_002716"
     CDS             complement(8977..9471)
                     /gene="ppiB"
                     /locus_tag="ON345_002716"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR3072754.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            9645..11030
                     /gene="cysS"
                     /locus_tag="ON345_002717"
     CDS             9645..11030
                     /gene="cysS"
                     /locus_tag="ON345_002717"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR3072755.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(11066..11587)
                     /locus_tag="ON345_002718"
     CDS             complement(11066..11587)
                     /locus_tag="ON345_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3072756.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(11695..11907)
                     /gene="ybcJ"
                     /locus_tag="ON345_002719"
     CDS             complement(11695..11907)
                     /gene="ybcJ"
                     /locus_tag="ON345_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR3072757.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(11909..12775)
                     /gene="folD"
                     /locus_tag="ON345_002720"
     CDS             complement(11909..12775)
                     /gene="folD"
                     /locus_tag="ON345_002720"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR3072758.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         2970 a         3437 c         3640 g         2942 t
ORIGIN      
        1 gccttcttct tctgccatcg ccaccacttc gaccgtacgt agcggcgcgc cgtattgcgt
       61 tttcagccag tcaattgcca gccacgccgc cagcgcgccg aattgcccgt cgaggttacc
      121 gccgttaacc acggtatcga tatgcgaacc gctcagaatc acttcctgtg gatattcggt
      181 accattcagg cgaccgtata aattccctac ttcatcgaaa cgtgtttcca gcccacttgc
      241 tgccattctt tttttaaatt gctgctgggt ttccagccat tccggcgaat aaagtaaacg
      301 ggtcatccca cccgttgggt cagcgccaaa agaggaaagc cagggcagcg ttttttctat
      361 ggcttgacga aaatgtgtaa tcataagaaa gtcctgtctc aataattatt gcgcaaaggg
      421 atttttcgtt tcgtatgacg tgttataaag cgcgtcggaa attaaatact ggtaaatatc
      481 atcaacaatt tcgatgcctt cgacggcggc tttgcgttgt ttaatatcct gatcctgtcc
      541 gggataataa acttggttaa aaccgggcgc gggggtaatg gcatttaatt cgcgcatggt
      601 ctggctaaga tgttgacgga ataattcgct ggaggagaaa aagttcggat taataactat
      661 atgtaattgc cccaaattac gccctgcgtg taaatcgtca tacatcgaac taacctgtcg
      721 cccgaacggt aagccgagta agacgcctga gaggacgtca atcatcatca tcaggccata
      781 cccttttggc ccggcggcgg ggagcagagc atgtaccgcg aacggatcgg ttgttggtgc
      841 accgttttta tcgaccgccc aggtatccgg gatagacata ttacgcgagc gggcgtcgag
      901 cacttttccc catgcctgta cggtagtcgc catatcaaag gtaaggatct cgtcgccttc
      961 tcccggcgcg gcaaaggcca gggggttagt accgtagtaa atttccgcgc cgccaaacgg
     1021 caccaccatt ggatcggact ggcacatcga aatgccaatg aatccggcgc gggctgcctg
     1081 ctgcacaaaa taagagattg cgccgctgtg acccatccgg ctgataccga ccaccgcaat
     1141 gccattttgc tgggcggttt tgatggcatg ttccataccc attttcgccg cgacctgtcc
     1201 ggcggcattg tcggcatgta aaattgccga gcacggcccg gtttcctcaa gacgaaactc
     1261 cggttcgcgg ttggtgccgc cttttgaaat gcgttccgcg tagtattcca cgcgcaccgc
     1321 gccatgagag tggatccctc tggcatcggc gtaaaccaat acttcagcca tggttgcagc
     1381 gtgctcacgt tttaacccag cctggcagag tttattctca attagctggt ggagtgtttc
     1441 ccgactgatt ttcatctgtc ttccttttta acgacggtgt gaagcatgac tgcaattaac
     1501 atacagggaa aatatctgga ttatgtgatc cagacaggca aaaaaatata gttagaattt
     1561 atttgataat ccgctcgctt ttaacctggt ttttaaaaca accacgctta ttaaaaaata
     1621 acacgtaata gcatggcggt tatttgaatt cttttgttaa taatccctgt gtgatattta
     1681 tcaccttatt tacccgttgt catcgatacc gtaatcgcca cattaacact gctcgtgcaa
     1741 ttgccatggg tgcaattttt aaggagttgt tatgatccac gcctttatta aaaaagggtg
     1801 ttttcaggat tcggtcagtt taatgattat ttcacgaaaa ctcagcgaat cagaaaatgt
     1861 tgatgatgtt tccgtaatga tgggtacgcc cgccaataaa gcgttattag ataccacagg
     1921 tttctggcat gacgatttta ataacgccac gccgaacgat atttgcgtgg caattcgtag
     1981 cgaagcggcg gatgcgggga tcgcgcaggc gattatgcag cagcttgaag aggcgctaaa
     2041 acaactggcg caggggtcag gcagcagcca ggcgttgacg caggtgcgtc gctgggacag
     2101 cgcctgtcag aaattacccg atgccagtct ggcgctgatt tcagtggctg gcgagtatgc
     2161 ggcggagctg gcgaaccagg cgctggatcg caacctcaac gtgatgatgt tctccgataa
     2221 cgtcacgctg gaagatgaaa tccaactcaa aacccgcgcg cgggaaaaag gcttgctggt
     2281 gatggggccg gactgcggta catcgatgat tgccgccacg ccgctggctt ttgctaacgt
     2341 gatgccggaa ggcaacattg gcgtcattgg cgcttccggt accgggattc aggagctgtg
     2401 ctcacagatt gcgctggcag gggagggaat tactcacgcg attggccttg gcgggcgcga
     2461 cctcagccgt gaagtgggcg gcatcagtgc gctgacggcg ctggaaatgc tcagtgcaga
     2521 cgagaaaagc gaagtgctgg catttgtttc aaaaccacct gccgaagctg tgcgtctgaa
     2581 aattgttaat gccatgaaag caaccggcaa accgacggtg gcgctgtttt taggttatac
     2641 cccggcggtg gcacgcgacg agaatgtctg gtttgcctcc tcgctggatg aggccgcacg
     2701 cttggcttgc ctgctttcac gcgttacggc gcgacgtaac gcgataacgc ctgccagcag
     2761 cggatttatt tgcggtttgt ataccggcgg tacgctggct gccgaagtgg cgggattact
     2821 tgccggatac cttggcgtgg aagccgacga tacccatcat cacggcatga tgctcgacgc
     2881 cgatggtcac cagattattg acctcggcga tgatttctac accgtcgggc gtccccatcc
     2941 gatgatcgac ccagccttac gcaaccagtt aattgccgat ctcggcgcta aaccgcaagt
     3001 gcgcgtattg ctgcttgatg tcgtgattgg cttcggtgcg accgccgatc ctgccgcctc
     3061 gctggtaagc gcctggcaaa aagcatgtgc cgcgcgttca gacaatcaac cactgtatgc
     3121 cattgccacg gtgacaggca ctgaacgtga cccgcaatgc cgctcgcagc aaatcgccac
     3181 gctggaagat gcagggatcg cggtagtgag ttcgctgccg gaagccacct tgctggcggc
     3241 ggcactgatt cgtccgcttt cgcctgccac tcagcaacac acaccgtcat tactggaaaa
     3301 cgtcgccgtg attaacagcg gattacgcag ctttgcgctg gagctacaaa gcgccagcaa
     3361 accggttgtg cattaccaat ggtcgccagt cgccggcggc aataaaaaac tggctcgttt
     3421 attagaacgt ttgcaataag gggttcccat gtttacatca gtggcgcaag ccaatgctgc
     3481 ggttatcgaa caaattcgtc gcgctcgtcc acactggctg gatgtgcaac cggcttcttc
     3541 acttatcagc gaactaaacg agggcaaaac actgcttcac gccgggccgc caatgcgctg
     3601 gcaggagatg accggaccca tgaaaggggc gtgcgtgggc gcatgtctgt tcgaaggctg
     3661 ggcgaaagat gaagcgcagg cgctggcaat actggagcag ggggaagtga acttcattcc
     3721 ttgtcaccat gtgaatgccg tcggtccaat gggcggtatt acttctgcca gtatgccgat
     3781 gctggtggtc gagaacgtga ccgacggcaa ccgggcgtac tgcaacctca acgaaggtat
     3841 cggcaaagtg atgcgttttg gcgcttacgg cgaagatgtc ctgactcgcc atcgctggat
     3901 gcgcgatgtg ttaatgccag tattaagcgc ggcgctgggg cgcatggagc gcggtatcga
     3961 tctcacggcg atgatggcgc agggcattac gatgggcgat gagttccatc aacgcaatat
     4021 tgcttcctct gcactgttaa tgcgtgcgct ggccccacaa attgctcgcc tcgatcatga
     4081 taaacagcac atcgccgaag tgatggattt cctcagcgtg accgatcagt tcttcctcaa
     4141 cctcgcgatg gcttactgca aggcggcgat ggatgctggc gcgatgatcc gcgcaggcag
     4201 catcgtcacg gcaatgaccc gcaacggcaa tatgttcggg attcgggtaa gcgggctggg
     4261 cgaacgctgg tttactgcgc ctgtaaacac tccgcaaggt ctgtttttca ccggcttctc
     4321 gcaggagcag gcgaacccgg atatgggcga tagcgcgatt accgaaacct ttggtatcgg
     4381 aggtgcggca atgatcgcag cgcctggcgt aacgcgcttt gtcggtgcgg gtggcatgga
     4441 agcggccaga gcggtatctg aagagatggc ggaaatttac cttgagcgca atatgcagtt
     4501 gcagatccca ggctgggatt ttcagggcgc gtgcctggga ctggacattc gtcgcgtggt
     4561 agaaaccggc attacgccac tcatcaatac cggtatcgcc cataaagagg cggggatcgg
     4621 gcagattggc gcaggcactg tgcgggcacc gctggcgtgt tttgaacagg cgctggaagc
     4681 actggctgaa agcatgggga ttggttgagg aacgcgcaat gacgatcatc catcctctgc
     4741 ttgccagccg tagtgcaccg aattatcgcc agtcctggcg gttagcggga gtgtggcgac
     4801 gggcgattaa cctgatgacg gaaagcggcg aactgttaac gctgcatcgt cagggtagtg
     4861 gtttcggccc cggaggatgg gtgcttcgcc gcgcgcaatt cgatgcgtta tgcggtggat
     4921 tatgcggcaa tgaacgacca caggttgtgg ctcaagggat tcgcctcggg cgtttcacgg
     4981 ttaaacagcc acagcgttat tgtttgctgc gtattacgcc gccttcgcat cctcaaccgc
     5041 ttgcagctgc atggatgcaa cgcgcggagg aaaccggact tttcggacca ctggcgatgg
     5101 cggcaagcga tccgctgccc gctgaattac gccagtttcg tcactgtttt caggccgcgc
     5161 tcaatggcgt taagaccgac tggcggcact ggctgggtaa aggccccgga ttaacgccaa
     5221 gtcatgatga cacgttgagc ggaatgctgc tggcggcctg gtattatggc gctttagatg
     5281 cgcgcgccgg tcgcccgttt tttgcctgtt ccgacaatct tcaactcgtt accacagcgg
     5341 tgagcgtcag ttatttacgt tatgccgcgc aaggatattt cgcctcgcca ctcctgcact
     5401 ttgtccatgc tctgagttgc ccgaagcgta ccgctgttgc gattgattcg ctgctggcgc
     5461 tggggcatac gtcaggggca gatacgctgc tggggttctg gcttggccaa caattattac
     5521 aaggaaaacc atgaaaacac tggttgtagc tcttgggggc aacgccttac tccagcgcgg
     5581 tgaggcgctg acggcggaaa atcaatatcg caatatcgcc agtgctgtac ccgcgctggc
     5641 acgcctggcc cgttcttatc ggctggcgat tgttcacggc aacgggccgc aggtggggct
     5701 gctggcgtta cagaatctgg cgtggaaaga ggtagaaccg tatccgctgg atgtgctggt
     5761 ggcggaaagc caggggatga ttggctatat gctggcgcag agcttgagcg cacagccgca
     5821 gatgccgcct gttacgacgg tgctgacgcg cattgaggtt tcgcctgatg atccggcgtt
     5881 tttgcagcca gagaaattta ttggtccggt ttatcagcca gaagaacaag aggcactgga
     5941 agcggcttac ggctggcaga tgaaacgtga tggtaaatat ttgcgccggg tggtggcgtc
     6001 tccgcaaccg cgtaaaattc tcgacagcga agccatcgag ttgttgctca aagaggggca
     6061 tgtggtgatt tgcagtggcg gcggcggtgt gcctgtgacg gaagacgggg cagggagtga
     6121 agcggtgatt gataaagatc tcgccgctgc cttgcttgcc gagcagatta acgccgatgg
     6181 gctggtgatc ctcaccgatg ctgatgcggt atatgaaaac tggggaacgc cgcagcaacg
     6241 cgccattcgc catgccacac cggatgagtt agcgccattt gccaaagccg atggttcgat
     6301 ggggccgaag gtaacggcgg tgagtggtta tgtcagaagc cgtggtaaac ccgcgtggat
     6361 tggggcgtta tcgcggattg aagatacgct ggcgggcgaa gcagggacct gtatttcgct
     6421 gtagtgatga gagcatccgg caatcggtgc accatgcctg atgcgacgct tgacgcgtct
     6481 tatcaggcct acaatgggta ccggagcggt aagccggata aggcgtttac gccgcatccg
     6541 gcaatcaaat gagcacctgc ttaactgaac ttactctgcg cccaaatcac gccgctggca
     6601 tactctggcg gcagcagcgg gattaaggct tccagcgtcg cagtcagacg cgatgtgtcg
     6661 ctgtcggtca aattcagatg ccccacttta cgccccggac ggacttcttt gtcgtaccag
     6721 tgcagatgca ccagcggcaa tttcagccag tcataattca catcgctacc aatcagattg
     6781 atcatcaccg acggattatt cactactggt tgcggtaacg gcagatcggt aatcgcccgc
     6841 agatgcagct caaactggct gatgctggca ccgttttgtg tccagtgacc gctgttatgc
     6901 acacgcggtg ccagttcgtt gatcaacaga ccttgcgggg tgacaaaaca ctccatcgcc
     6961 atcacgccca catagcccag ctcctgcata atcgccgaca gcatctcttc ggcttgcgcc
     7021 tgctgctgtg cgttggcctg cggaaaagcg acgctggtgc gcaaaatacc gtcctgatgc
     7081 aggttatgcg tcagcggata aaacacggtg ctgccatcaa agccgcgcgc gccaaccagc
     7141 gacacttcac cagagaagtt aatgccctgc tcgacaatac attcgccgta acactctgcc
     7201 ggtaactgtt cggtttcatt tgcgcgtaaa cgccattgac cgcgaccgtc atagccacca
     7261 gtgcgacgct taacaatcgc cagttcacct aaacgatcaa acaccgcagg ccactcgctg
     7321 cgatcggcaa gtaactgcca cggcgcggtc ggcaggtgga gcttatcgaa aagctgcttc
     7381 tgagtcagac ggtcagcaat aatcgggaac acatcgcggt tcacaaaggc cggatgacgc
     7441 gccagctcgc gggttaatgc ggtttccggc cagcgttcga tctcagcggt aatcacgctt
     7501 tgttgaaaag gcaccgccgc cggttcagcg tccagcccga ccggccagac agcaatgcct
     7561 aacggttcgc ctgcctgacg cagcatacgg cctaactgcc cgttaccgag gacgcaaacc
     7621 tgtttcatgc cgcacctcgc gggtccgggt tttccagcac ttcgtcggtc tgggctttgc
     7681 gccagtcatt cagacgctgg tgtagctctt tatcgtgagt cgcgagaatt tgtgctgcca
     7741 gtaatgccgc gtttgccgcg ccagctttac caatcgccag cgtacccacc ggaatgccgc
     7801 gcggcatttg tacgatggag tagaggctat cgacaccgct cagtgcggcg ctctgtactg
     7861 gcacgcccag caccggcacc agcgttttgg cggcaatcat gcctggcagg tgcgctgcgc
     7921 cacctgcgcc cgcaataatc acctgataac cgttctcttc ggcgctttcg gcgaagctga
     7981 acagtttatc gggggtgcgg tgagcagaaa ccacttcaac gtggtgcggg acattcagga
     8041 tttcgaagat ttcggcggcg aactgcatgg tagcccagtc gcttttggac cccatcacga
     8101 tggcgacacg cgccggatta ttgcgggaag acatgcgtct taaaactcct gtggtgcaca
     8161 actctcggct ttagagggca cagagaatag cacggaaaga gagcaaggaa aacggttgcg
     8221 tggctgtgaa atcagcaaag ttgtgggttt tttaaaacgg aaaatgaatc agctcaacgt
     8281 catccgccgt gactttcacc atcgaacctt ccgtatgcca ggcacccagt accacgcgaa
     8341 aagcaggttg ctggttggcg ataagttcat gcaccgccgg gcgatgggta tgcccgtgga
     8401 tcagccattg cacctgatat ttttccatcg cactgaccac agcgttttgg ttaacgtcca
     8461 tgatcgccag cgatttactg ctgttggctt ctttgctgtt cgcgcgcatt cgcgcggcaa
     8521 tgcgtttgcg cacaaacaac ggcagggcga ggaatagcat ttgcagccag ggtttgtgga
     8581 ctttggcgcg aaaagcctga taacccgcgt catcggtgca cagcgtgtcg ccatgcataa
     8641 tcaacacccg gcggccataa agttcgagca ccttttcttc cggcaataac gtcatgccac
     8701 tttcacgggc aaagcgtttg ccgagcagaa aatcacggtt gccatgaatg aaataacagg
     8761 gaacgccgga atcggacacc gctttgatcg ctgccgccat ctggcgatgg agtggattgg
     8821 gatcgtcgtc gccaatccac gcttcaaaca gatcgccaag aatatacagc gcgtcggcct
     8881 tgcgagcttc ccccgctaaa aaacgcagaa aaccggcggt gatcgccggt tcttccacgc
     8941 agagatgaag atctgcaata aagagtgtcg ccacgattac tcgctaacgg tcacgctttc
     9001 aatgataacg tcttcttttg gcacgtcctg gtgcataccg ctacgaccgg ttgcaacacc
     9061 tttgattttg tctaccacgt ccatgccttc aaccacttca gcaaacacgc agtagcccca
     9121 accttgcagg ctttcgccag agaagttcag gaagtcgtta tcaaccacgt tgatgaagaa
     9181 ctgtgcagtt gcagagtgcg gagcctgagt acgggccatt gccagcgtac cacgggtatt
     9241 tttcagaccg ttgttggctt cgtttttgat cggttctttg gtggcttttt gtttcatgcc
     9301 cggttcaaaa ccgccgccct gaatcataaa gccgttgata acacggtgga aaatggtgtt
     9361 gttgtaaaaa ccttcgcggc agtagtccag gaagttttta actgtttcag gtgctttatc
     9421 gtcaaaagtt ttgatgacaa tatcgccgtg attggtgtgg aaagtaacca tttttgcatc
     9481 ctgttccgtt tgattggtgc ttcaacccag ttcgggtcat atatagggtg gtgttatagc
     9541 ataaccgcac gatcggatca tcacgcaatg tatgctgatt cgcgcgggaa atatgggtat
     9601 tatacgcaac tcaattaccc acacatgtct aaacggaatc ttcgatgcta aaaatcttca
     9661 atactctgac acgccaaaaa gaggaattta agcctattca cgccggggaa gtcggcatgt
     9721 atgtgtgtgg aatcaccgtt tacgatctct gtcatatcgg tcacgggcgt acctttgttg
     9781 cttttgacgt ggttgcgcgc tatctgcgtt tcctcggcta taaactgaag tatgtgcgca
     9841 acattaccga tatcgacgac aaaatcatca aacgcgccaa tgaaaatggc gaaagctttg
     9901 tggcgctggt ggatcgcatg atcgccgaaa tgcacaaaga ttttgatgct ttgaacattc
     9961 tgcgcccgga tatggagccg cgcgcgacgc accatatcgc agaaattatt gaactcactg
    10021 aacaactgat cgccaaaggt cacgcttatg tggcggacaa cggcgacgtg atgttcgacg
    10081 tcccgaccga tccgacatat ggcgtgctgt cgcgtcagga tctcgaccag ctgcaggcag
    10141 gtgcgcgcgt tgacgtggtc gacgacaaac gcaacccgat ggacttcgtt ctgtggaaga
    10201 tgtcgaaaga gggcgaaccg agctggccgt ctccgtgggg cgcgggccgt ccaggctggc
    10261 acattgaatg ttcggcaatg aactgcaagc agctgggtaa ccactttgat atccacggcg
    10321 gcggttcaga cctgatgttc ccgcaccacg agaacgaaat cgcgcagtcc acctgtgccc
    10381 atgatggcca gtatgtgaac tactggatgc actcggggat ggtgatggtt gaccgcgaga
    10441 agatgtccaa atcgctgggt aacttcttta ccgtgcgcga tgtgctgaaa tactacgacg
    10501 cggaaaccgt gcgttacttc ctgatgtcgg gccactatcg cagccagttg aactacagcg
    10561 aagagaacct gaagcaggcg cgtgcggcgc tggagcgtct ctacactgcg ctgcgcggca
    10621 cagataaaac cgttgcgcct gccggtggcg aagcgtttga agcgcgcttt attgaagcga
    10681 tggacgacga tttcaacacc ccggaagcct attccgtact gtttgatatg gcgcgtgaag
    10741 taaaccgtct gaaagcagaa gatatggcag cggcgaatgc aatggcatct cacctgcgta
    10801 aactttccgc tgtattgggc ctgctggagc aagaaccgga agcgttcctg caaagcggcg
    10861 cgcaggcaga cgacagcgaa gtggctgaga ttgaagcgtt aattcaacag cgtctggatg
    10921 cccgtaaagc gaaagactgg gcggcggcag atgcggcgcg tgaccgtctt aacgagatgg
    10981 ggatcgtgct ggaagatggc ccgcaaggga ccacctggcg tcgtaagtaa ttgcgctgtt
    11041 gccggatgcg agttttcgca tccggttatc gcctgcgcca ccacaacatt cccataagca
    11101 ccatccccgg caaccacacc cacatcaatt cagaaattat cacctgatgc ccgtacggcg
    11161 tggtgtaacg agacaatgca aacggcgcga ctttaatcac ctgccaggga gcgaaaaagc
    11221 gttcatctga ccacggccac agccagccaa cgcccttacc gccagtggtt accgaatcca
    11281 gcaagctgtg cgatagcaac gagacggtta aaaacagcca gcagcgaatc agcccagccc
    11341 tgaaccatcg gcgtccaatc agcacacata acagcgggac aacaaacgca aacaccagcg
    11401 aatgggtaaa cccgcgatga ccaaaaacat tgccgtaagc aacgccaaat ttaaacgaca
    11461 atacgtcggc gtcgggcagc atcgccagga tgattccggc aaataacaga cgcggaggga
    11521 tgactttcga acccaaccct aaaccaatgc ataggggaac ggcggcgtgc gtaataacgg
    11581 ttggcatgat ggtcgcttcg gcaaaatgtc gatgctatca gcatggatga acggggcgta
    11641 gagggcaaaa gtctgaaaag agaaccggcc tgttgataca ggccgggaaa gggatcaggc
    11701 aacaacctgt acgctgtgac ctgcaaaact cactgtctga ccggcgacga ttttgcagcg
    11761 tttgcgcgtt tcaaccgcac cgtcgacttt cacctggcct tcggcaatcg cgattttcgc
    11821 ctgcgcgccg ctttcgctcc agccttccag tttcagcaag tcgcacagct caacgtgcgg
    11881 atgtttacct aaagaaaatg tcgccatgtt actcatcctg tggatcatga tattcaacgc
    11941 acgcctgtag cgtgttttca atcagcgtgg caaccgtcat cgggccaacg ccgccgggaa
    12001 caggcgtaat gtatgaggcg cgtttagccg cgtcttcaaa cacgacgtcg cccacaactt
    12061 tgccattttc cagacggttg atgccgacat caatcacaat tgcgccttct ttgatccagt
    12121 caccgggaat aaagcctggc ttgccaacgg caacgatcaa tagatcggca ttttctacgt
    12181 gatgacgcag atttttagtg aagcggtgag tcacggtagt ggtgcaacct gccagcagca
    12241 gttccatgct catcgggcgg ccaacgatat tcgatgcgcc aatcaccacg gcgttgaggc
    12301 cgaaggtatc aatgttgtaa cgctcaagca gcgtgacgat accgcgcggg gtgcagggac
    12361 gcagacgcgg cgcgcgctgg cacagacgac cgacgttgta aggatggaaa ccgtccacgt
    12421 ctttgtccgg atgaatacgt tccagcactt tgacgttatc aatacccgcc ggtaacggca
    12481 gttgaaccag aatgccatcg atggtgttgt cggcattcag cgtatcgata agctccagca
    12541 gctccgcttc gctggtggtt tccgggaggt cataagagcg ggagacgaac ccgacttctt
    12601 cacaagcctt gcgtttgctt gcgacataaa tttgcgatgc agggttacta cccaccagca
    12661 caacggccag tcctggtgcc cgcagtccgg ctgcaatacg cgcctgaact ttttgagcaa
    12721 cttcagagcg cacctgctgc gcaatcgttt taccgtcaat aatctttgct gccatcagag
    12781 agaggattcc atctgttacg taggtcgaag gggatgcgcc tattttgtca gaagcggggc
    12841 gcgctgtcag gtttcgtttc agatttatcg cgtgaagcga cctcttgcga aggttaggcg
    12901 caccgtcgct gaggtcgctg agattgaaag cttcattttt cgtccatgat ggcgttgtaa
    12961 atctggaact gatttatttc cttgtttaa
//
LOCUS       DAJSKJ010000114        12745 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000114 DAJSKJ010000000
VERSION     DAJSKJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.114"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            139..1905
                     /gene="adeD"
                     /locus_tag="ON345_002721"
     CDS             139..1905
                     /gene="adeD"
                     /locus_tag="ON345_002721"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR3072759.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1950..3341)
                     /gene="uhpT"
                     /locus_tag="ON345_002722"
     CDS             complement(1950..3341)
                     /gene="uhpT"
                     /locus_tag="ON345_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR3072760.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3479..4798)
                     /gene="uhpC"
                     /locus_tag="ON345_002723"
     CDS             complement(3479..4798)
                     /gene="uhpC"
                     /locus_tag="ON345_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR3072761.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4808..6310)
                     /gene="uhpB"
                     /locus_tag="ON345_002724"
     CDS             complement(4808..6310)
                     /gene="uhpB"
                     /locus_tag="ON345_002724"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR3072762.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IISHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6310..6900)
                     /gene="uhpA"
                     /locus_tag="ON345_002725"
     CDS             complement(6310..6900)
                     /gene="uhpA"
                     /locus_tag="ON345_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR3072763.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6975..7265)
                     /gene="ilvN"
                     /locus_tag="ON345_002726"
     CDS             complement(6975..7265)
                     /gene="ilvN"
                     /locus_tag="ON345_002726"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR3072764.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7269..8957)
                     /gene="ilvB"
                     /locus_tag="ON345_002727"
     CDS             complement(7269..8957)
                     /gene="ilvB"
                     /locus_tag="ON345_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR3072765.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWVDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVSNAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9063..9161)
                     /gene="ivbL"
                     /locus_tag="ON345_002728"
     CDS             complement(9063..9161)
                     /gene="ivbL"
                     /locus_tag="ON345_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR3072766.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9727..9816
                     /gene="tisB"
                     /locus_tag="ON345_002729"
     CDS             9727..9816
                     /gene="tisB"
                     /locus_tag="ON345_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR3072767.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10234..11418
                     /gene="emrD"
                     /locus_tag="ON345_002730"
     CDS             10234..11418
                     /gene="emrD"
                     /locus_tag="ON345_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR3072768.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11426..11923)
                     /locus_tag="ON345_002731"
     CDS             complement(11426..11923)
                     /locus_tag="ON345_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3072769.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11920..12282)
                     /locus_tag="ON345_002732"
     CDS             complement(11920..12282)
                     /locus_tag="ON345_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR3072770.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12272..12619)
                     /locus_tag="ON345_002733"
     CDS             complement(12272..12619)
                     /locus_tag="ON345_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR3072771.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
BASE COUNT         3037 a         3551 c         3335 g         2822 t
ORIGIN      
        1 ttgtaaaaca aatgacaaca acggttcagt gataattatc acatttcaat tgcacattaa
       61 tggatattct ttaataatct cgcgacgttt ctttatgata aataataatc aaattgataa
      121 aatcaaaatg agaaaaatat gaataattct attaaccata aatttcatca cattagccgg
      181 gctgaatacc aggaattgtt agccgtttcc cgtggcgacg ctgttgccga ttatattatt
      241 gataatgtct ctattctcga cctgatcaat ggcggagaaa tttccggccc aattgtgatt
      301 aaaggacgtt acattgccgg tgttggcgca gaatacactg atgctccggc tttgcagcgg
      361 attgatgccc gcggcgcaac ggcggtgcca gggtttattg atgctcacct gcatattgaa
      421 tccagcatga tgacgccggt cacttttgaa accgctaccc tgccgcgcgg cctgacgacc
      481 gttatttgcg accctcatga aatcgtcaac gtgatgggag aagccggatt cgcctggttt
      541 gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac aagtcagctc ttgcgtaccc
      601 gccctggaag gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg
      661 cgggaccatc cgcaggttac cggccttgca gaaatgatgg actaccctgg cgtaattagc
      721 gggcagaatg cgctgctcga taaactggat gcatttcgcc acctgacgct ggacggtcac
      781 tgcccgggtt tgggtggtaa agaacttaac gcctatatta ctgcgggtat tgaaaactgc
      841 cacgaaagtt atcagctgga agaaggacgc cggaaattac aactcggcat gtcgttgatg
      901 atccgcgaag ggtccgctgc ccgcaatctc aacgcgctgg caccgttgat caacgaattt
      961 aacagcccgc aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa
     1021 ggacacatcg atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg
     1081 gcatatcgcg tcgccagctg gtcgacggcg cgccactttg gtctgaatca cctcggctta
     1141 ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg
     1201 cagcaggtac tggtgaaagg cgagccgatt gatgcgcaaa ccttacaggc ggaagagtcg
     1261 gcgagactgg cacaatccgc tccgccatat ggcaacacca ttgcccgcca gccagtttcc
     1321 gccagcgact ttgccctgca atttacgccc ggaaaacgct atcgggtcat tgacgtcatc
     1381 cataacgaat tgattacgca ctcccactcc agcgtctaca gcgaaaatgg ttttgatcgc
     1441 gatgatgtgt gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcttgt
     1501 ggtttgcttg gcggctttgg actgaatgaa ggtgcgctgg ctgcgacggt cagccatgac
     1561 agccataata ttgtggtgat cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag
     1621 gtgattcagg atggcggcgg gctgtgcgtg gtacgtaacg gccaggtcca aagtcatctg
     1681 ccgttgccca ttgccggcct gatgagcacc gacacggcgc agtcgctggc ggaacaaatt
     1741 gacgccttga aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag
     1801 atggcgtttc tttctctgcc agtgatcccc gcgctaaaac taaccagtca ggggctattt
     1861 gatggcgaga agtttgcctt cactacgctg gaagtcacgg aataataaaa aagcccggcg
     1921 tcatgccggg caaaagtcac cagttacgtt tatgccactg tcaactgctg aatttttttc
     1981 tcacggcgga ttttgcgttc ttccatcacc gccactatcg ccatcaggca gatacaacca
     2041 atcgcggcga tatcaagtgc ggcgaaggtg cctgcccagc cggtaaggcc gaataccggc
     2101 gtcccatcgg caatcattcc cagacctaac ttggcaaagc tgtcaccaat caggtaagca
     2161 aaggtgcctt taataccatc ggcagcgcca atcgcttttt taggtacaaa gccaacagca
     2221 gccacaccaa tcaacaattg cgggccaaag accaggaaac ccaaagcaaa gagagaagcc
     2281 agatagatat attcgttgct ggcatgttga tacacaccga gcgtggcgat aatcagcgcc
     2341 agcgcgatgc aggccaccag gccacggcga ccgttcgcca ggtcagagag ccagccccac
     2401 agcagcgtac cgaccagcgc accagcttca aacagcgtaa agccctgaat cgccaccgct
     2461 ttagagagtt tcagttcctg gaacgcgtat acggttgacc actggtcgat accaatacgt
     2521 accacataga ggaaaatgtt ggcgaagcac agcagccaga tcactttgtt tttcagcaca
     2581 tactcaacaa agatctgcca cttggtcata tcggtagatt ctgtctcttt gtcctcttcg
     2641 ctgatctcct cgccgaacag ttcttcagct ttgccgaggc cataagattc cggggagtcg
     2701 ctgccgtaac gcaggccgat aaaaccgaca atcagcgcga taatcgacgg gaagataaac
     2761 atgccgatga catggccatc gaacaggtaa tttgccccga acagcgccac acctgctgcg
     2821 cctgcaccgc caaggttgtg agaaatattc cagaaaccga ggaatgtccc gcgtttacga
     2881 cgcggcgtcc atttggtgat ggtggagtaa ctgcacgaac cgccggtact ctggaaaaag
     2941 ccgcttaagg cgtagaaggc aatcatcagg aacaggctaa ccgagccgct gcccatactg
     3001 gcactgaagc ccagcataca aatggcagac aggatcagca tgaacggcag gaattgtttg
     3061 gtgtttttgc cgtcggcgta gtaggaaacc agcgttttac ccacgccata agtgatggag
     3121 aaacccaggc cgatcatccc cagctgcgtc atgctcaacc cgtaggtcga gatcatgtcg
     3181 ttctgcgcga tgttaaagtt cttgcgaatc aggtacatcg tcaggtagcc gataaagacc
     3241 accaggtagg attgcatgaa cggtttgaac cacattttgc gccgcacttc gagcggaagg
     3301 tccagggtcg gcttgcgaac ctggtttaag aaagccagca tgggttactc ctgaaatgaa
     3361 tacctgcgtg aggcatgcat tgtaaaaatc agcagcaaat caggcctgag atagcgtcca
     3421 ggcaaaacct ggaaaatttc ttagttttgc cggatatgaa gtgaaaaggt gagatgcatc
     3481 acgcttcgcg cggtgtctgg gcgttcaaaa agggcagtaa cagcagtgcg gaaatcccgg
     3541 cggcgataga gataaccaca aaaaatccgc tccagtgcca ggtatcgagt actttcgcca
     3601 gcggccaacc agcaagcgac gcccccagat aagcaaacaa gccgacaaac cccgtcgccg
     3661 cccctgccgc ctctttgtgg gaacactctg ccgccgccat accgattaac atctgtgggc
     3721 caaagacaaa aaaaccaatg gtgaagaagc aggttgcctg catcacgtag ctggcaaatg
     3781 gcatcagcca cagggagccg actgaaagca aaattccggc ggcgaaaatc aaattcatcg
     3841 gccctcggtt gccgttaaac aatttgtccg agccccaacc cgctaccagc gcaccgataa
     3901 atccacccag ttcaaacatc gtcactgccg tattcgccgt gaccagatcg acgcccagcg
     3961 tctcggacat atacaaattg ccccagtcgt tgatcgccgc ccggaccaca tagaccagca
     4021 catagcaaaa cgaaagcagc cagatatacg gattcagcaa cacatatttg gtgaggatct
     4081 ctttacgcgt caaccctgcc ccttcttgtt gttgagcaat ttccagcgcg tcgtgccgcc
     4141 attcaccgac cgccggtaaa cctaacgcct gcgggcgatc gcgtagccgc cagcagagaa
     4201 aaatccccac gactatcgcc atacaaccag caatcatcat cccggcacgc cagccgtaat
     4261 gcagcgcgct cgctgccatc acaatgggaa tgagtgcgcc gccgacgtta tgcgccgtgt
     4321 tccataatgc ccaccaaccg ccgcgctcgg tacgtgaata ccaggccgtt aacagacgcg
     4381 cgcacaccgg tgaaccccag ccctggaaaa aggcgttcag cacccagagt acggcaaacg
     4441 cccatagcga cgtcgagaag ccaaacagaa tgttgataat gcccgtggcg ataagcccta
     4501 tccccataaa ataacgggca tttgagcgat cgctgacaat gccggagaca aacttcgaca
     4561 cgccataggt aatgtaaaac agggtcgcta acaggccgat atcgctacgg ctgagcacgc
     4621 cgttagcaag gatttctggt acggcggcgt taaaactttt ccgcgtgaag taaaacagcg
     4681 cgtaacccag ccagatggtc agcagaatat gccgacgcca gtagcgatag cgggcatcaa
     4741 tttcatattt atcagtcatt aatggcgcat cggcaggcgc tttcagaaac ggcaacatca
     4801 tcaaacctta gacatagcgt tgaggtagag aaacgctgac acgcgtgccg tgcagacagg
     4861 aaatgtgtaa tgtgccaccc agcgccgtta cgcgctcgcg cattccggtg aggccaaaac
     4921 cttgttgccc ggaacccggc ggcaaaccgc tgccatcgtc ttcaataacc agcatcaacc
     4981 gttcatcctg ctgccagcct tgcagggtga ccgcgctggc atcagcatgt ttcacaatgt
     5041 tgttcagccc ttcctggcag acacgaaaca gcgtcacgcg ctggttttcg cttaacgctg
     5101 attcatcgat tcgccattcg agatggctga taataccgcg cccttccagc tccatttccc
     5161 gcatcagtga gcggatggcc tgctccaggg tgagatcatc caactggcgc ggacgtaacc
     5221 gacccaacaa acggcgcacc gcgtcgtaaa cgcccagcga tagttgttcg atgagctgcc
     5281 cgctctgctt cacgctggcg ttatctgccg ccagccgctg aacaatgccc gcctgagtac
     5341 gaatagcagt gatggtctga ccgatatcat catgcagctc acgcgccaca tcacggcgca
     5401 cgctctcttc ggtttccagc aaccgttcag ccagatgctg attgcgcgcc agttcctttt
     5461 gcagcgactg gttaagttca cgcaaccgct ggatgccagc gccaagcaac aaccctgtca
     5521 gactttgcac cagcagcgag agcaataaat ccaccggatg atcgcgccag gtttgactgg
     5581 cgatcagcgc gatggcgttc atcaacgtcg caatcagcgc cccttgccaa ccatagtgcc
     5641 aggccagcgc gataatcggc agcgccagac agaatggcgt aaagcgcgac agttcgtccg
     5701 gcaatcccaa ctggagccag agactgataa caaacagcag caagtaccag accagatgtc
     5761 gcccgcgcca gttgattggc tgagaaacca gtgacgggcc gagcggcagc caggtgttat
     5821 tggcgagata gtgccagaac accagacata tcggggccag cgtcaggccg ccagttaaag
     5881 tcagcaacag cgcattccac gactctttgc cgtgccaaag ccagggcagc gactgcaaca
     5941 acgccgccgc cgttaacgcc gccccctgta acagcaaggt gcgccagtca cgctgatggc
     6001 gatagcgcga gatcagcgct acgggcagta acgtcagtaa actaccgatc atcaataacg
     6061 gaaaatgggt taaaccgacc gcctgcgtta gccagtaaat cagcagccac tccgcaccca
     6121 gcaatacggg ccagtatccg cgcgggcatt gcagcattag ccccagacgc agaccaaacg
     6181 gaaataacag caccgccata tcagggcgct caaccagatg caggctgata ctccacaggc
     6241 aaaaccatgc ggcagagaag ataaaaaagc aggcaataac ggtaattaag cgggagaaca
     6301 acgtcttcat caccagccat caaacatgcg gcgcgccagc tctacgtcgt tactgacgcc
     6361 cagtttttcc atcagattgg cgcgatggac gtgtaccgtt ttcggtgaca agcccagttc
     6421 ggcggcaatc tctttcaccg ccattccttg cgccagtttt tccgccacct ggcgttcacg
     6481 tttggttagc gggtcctgac gaccggatgc cagtttaatg gcaatatccg gcgtcagata
     6541 acagccgccc gtggcaaccg tatgcaccgc agcaatgagt tcatccgggc tacagcgttt
     6601 ggaaagaaag ccgcgtgccc ccgcgttaag cgcctgctca accagcgcag gactgtcgtg
     6661 aacggagagc ataatcgtcg ccataccttt cggcagctgg cttagcagct ccagaccgga
     6721 gatatcgggc atggagatat cgcaaataca cacctgcaca ccgcgccccg gcagccccgc
     6781 cagcgcctcg cgccccgaac caaactcggc aactacctgc aaatcaggtt ccagccccag
     6841 cagctgcgca aagccggagc ggacgatgag gtgatcgtct ataagggcaa cggtgatcat
     6901 ggtcttgtcc tggcgggtaa aaaaaaacgc gcttacctta acgataagcg agatgttgtt
     6961 caagccttga gcggttactg aaaaaacacc gcgatcttgt taaacatcgt cggatcggac
     7021 tgattacgct gcactttcac gacatcttcc agcttatcga tttggcttat catctgctcc
     7081 agacgctggt cgtcattgac cagtagccag atatggcttt tgtcgctgtc ctgaatcggc
     7141 agacaaagaa tgccttcaac gttaaaagcg cggcgggcaa aaaggccaca aacgtgggtc
     7201 attacgcccg gatggttgcg aacggtgagc tccagaatta cgttgttatg agttgtgttt
     7261 tgcatggctt attcccccac catttcagta ttcgccgcac ctggcggcac catcgggtaa
     7321 actttttctt cggcatcaat gcgcacatgg atcagcgccg ggccagggcg attgatgatt
     7381 tcctgcaatg cagcctgcgg atcggcttcg ttattcaaat cacaggtttc gaggccgaat
     7441 ccggcggcaa tctgcataaa gttgattttg cccggatagg tggcggcaaa aacgccttgc
     7501 tcgtagaaca gactctgttg ctgatgcacc agccccagcg cttcgttgtt catcagaatg
     7561 attttgacat ccagctgatt ttcactggcg gtcgccatct cctgaatatt catcatcagg
     7621 ctgccgtcgc cggagaaaca caacactttg cgatccgggt tcgccagcgc cgcgccaatc
     7681 gccgcaggca ggccaaaacc catcgtgccc agcccaccgg aggtcagcca ctggcgtggg
     7741 cgattgagcg gataagcttg cgcggtccac atctgatgct gacccacatc ggtggtgata
     7801 atcgcattgt catcgacaca ggcggcaacg gcgttgatca ggccgtaatg gcttaatgga
     7861 tcgcacgctt tcgggattgg acacgggaat tcgcgctgca aatccgctac caactggtgc
     7921 cactctgcac gcggttgcgc ttccacctgc gggatcaatt gcgccagcac gtcatcaaca
     7981 tccgcctgaa tcgccacatg cggctgcttg attttaccca gctctgcgcg atcgatatcg
     8041 acatgaataa ttttggcatt cggacagaac tgctcggttt tgccaatcgc cctgtcatca
     8101 aaacgcgcac cgagcacaat caacaaatcc gcctcctgca aaatatagtt ggtactgcgc
     8161 acgccgtgca tccccagcat acccagcgac aacggatgcg cttttggcag catgcccagc
     8221 gccattaaag tcatggtggt aggcagttgc gctttctccg ccagttcacg cacccgcgca
     8281 ggcgcattgc tcacaccacc acccagataa agcaccgggc gtttggcggc gttaatcatc
     8341 gcagccgcgt cacgaatgct ttcttcgcta aaggcggggg cggcggcttt ttctgccacc
     8401 gcgggctgtg cttcaatctc aaaaaccgcc gtttgcacat ccttaggaat gtctacccac
     8461 accgggcctg ggcggcctga ttgcgcaatg cggaaggcat cgctcatgac ctgcgggagt
     8521 tcttcgatat gtctgaccag atagttgtgt ttggtgatgg ggatagagat gccgtaggtg
     8581 tcgacttcct ggaaggcgtc ggtgccgatc atcgaggcgg gaacctgacc agtgatgcaa
     8641 atcagcggga tggagtccag ccgcgcatcg gcaatggcgg tcaccaggtt agtcgcaccc
     8701 ggtccgctac aggccataca gaccgccggt ttaccgtcgg tgcgcgccat tccctgagcg
     8761 ataaagcccg cgccctgttc atgacgggcc agaatatggc ggatttgcgt gctttggctt
     8821 aaggcatcgt aaacaggcag gatagaaccg cccggaatgc ccgtcacaat cttaatgccc
     8881 tgctgttcca ggaaatgaac gataaattct gcgccggtaa agcgcttacg cgtcgatgtt
     8941 gtgcccgaac ttgccatgct ccagtccttt tcttctgggc cgactttccg ggaggtgctt
     9001 aaacgaaaaa ccccgcccgg tttgcgccgg cggggttttg gaatcgtgtg ttgttccagt
     9061 ccctacggcg cattgccgac gaccaccacc acacgcacga cgaccactgc ggcggatagc
     9121 gcagttggta gtagttttgc gttgaacatg gaagtagtca ttggggacct tgttggtttt
     9181 gtgtttaaca atatttatac aagcacagct ttacagggga gacaatggaa aattttttca
     9241 gcaagggaaa attgaggggc tgatcacgtt ttgtactgaa ttgcaggtaa caaaaaaccc
     9301 cgccggagcg aggtttcatc agccgcctgc ggctggtaac cgcaaagcac actgtattat
     9361 gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
     9421 gtatttaggg catagttgag tatcgatcac agtttgcatt ttgtccaaat attactgttt
     9481 atttatacag taaacttcta taatatcact gtacgcaatg tgttatgcgg gggccgcatc
     9541 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
     9601 gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca
     9661 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
     9721 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
     9781 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
     9841 ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggagttgg ggcttcttct
     9901 accagcagaa tgtttgcacc tgcgctgcct gtgcccctca ccctagccct ctccccaaag
     9961 gggcgagggg accgtcccgt gcaatgttta gctcattttc tgaaagggaa cggtggtttc
    10021 cccctcgtcc tttcagggag agggttactg ttcgcacgta atccctcccg gcaacattta
    10081 ttgccgcttt tgtttacata ttctgccgct aaacaattcc ccattcctgg cgtatatctg
    10141 gctaacattc atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt
    10201 attctaatta tttttataaa agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg
    10261 ttattgatgt tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca
    10321 gctattgccg atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg
    10381 ggcgcttatc tgctgactta cggtgtctca cagctgtttt atggcccgat ttccgaccgc
    10441 gtgggccgcc gaccggtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc
    10501 gcggtcacga cctccagttt gacggtgttg attgccgcca gcgcgatgca ggggatgggc
    10561 accggcgttg gcggcgtaat ggcgcgtact ttgccgcgag atttatatga acggacacag
    10621 ttgcgccatg ctaacagcct gttaaacatg gggattctcg tcagtccgtt gctcgcaccg
    10681 ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg
    10741 gttctttgtg ctggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc
    10801 gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt
    10861 aactgttatt tgctgatgct gattggcggt ctggccggga ttgccgcctt tgaagcctgc
    10921 tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt
    10981 attctgccga ttccggcagc gttttttggc gcatggtttg ccggacgtcc caataaacgc
    11041 ttctccacgt taatgtggca gtcggttatc tgctgcctgc tggctggctt gctgatgtgg
    11101 atccccgact ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc
    11161 tttttcggtg ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc
    11221 ttcctggcgg gcacggctgg cgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg
    11281 ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg
    11341 accttaatgg gattgttgat cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat
    11401 caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt catcagctcc atgggagaac
    11461 gatgctgctt tatcagatca cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg
    11521 cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg gcgagcgtgc gggcacaggc
    11581 gatgccagtc gcacgcctgg cagtcacggc gcggttgcgg gttttcgctg ccacaccatt
    11641 tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact gatatctgcc cgcacccaac
    11701 actcgaacag ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca
    11761 gatcgccccg gtcatcaaat tcaagatagg gaatgagctg tacggagcgg atgccccgct
    11821 gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagtt
    11881 tatgcctgtc ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa
    11941 tacgataagt tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag
    12001 ggagatcaaa aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc
    12061 cattaaattg cgattacggg tgtagtgcca gaggatcagc gccacgatgg cgagaatgcc
    12121 aatcgaaatc cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat
    12181 caacgcgccg tagcccagca tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag
    12241 tcctgggtcg gcaatgcgtc tggctttgcg gctatccggc atacaacacc agtcccataa
    12301 cgatcaccgc cacgaccatc aatattaagc taatgattaa taagctgttg gtgtagggca
    12361 ggtcctcttt cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacatcg
    12421 ctaacccgcc ggaaaacaga cacaacaaca acgccaacag ttcacgaatg acgggcgtgg
    12481 cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc cagaaatcct agtgcggtac
    12541 ggatccacgc cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc
    12601 caaggcggga aattttcatt gttgctcctt tgcaatttca ctaacgctct atctttagcg
    12661 gtttttttta agaatttcgt ccgcccgttt cctgatatgc ttttgccatc cgctacaagg
    12721 aggtcagatg ggtatcatag cgcaa
//
LOCUS       DAJSKJ010000115        12680 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000115 DAJSKJ010000000
VERSION     DAJSKJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.115"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            281..493
                     /gene="cspG"
                     /locus_tag="ON345_002734"
     CDS             281..493
                     /gene="cspG"
                     /locus_tag="ON345_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR3072772.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            504..692
                     /locus_tag="ON345_002735"
     CDS             504..692
                     /locus_tag="ON345_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3072773.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            667..897
                     /gene="ymcE"
                     /locus_tag="ON345_002736"
     CDS             667..897
                     /gene="ymcE"
                     /locus_tag="ON345_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR3072774.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            887..1060
                     /locus_tag="ON345_002737"
     CDS             887..1060
                     /locus_tag="ON345_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR3072775.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1109..2182)
                     /locus_tag="ON345_002738"
     CDS             complement(1109..2182)
                     /locus_tag="ON345_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR3072776.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2254..4998)
                     /gene="torS"
                     /locus_tag="ON345_002739"
     CDS             complement(2254..4998)
                     /gene="torS"
                     /locus_tag="ON345_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR3072777.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSANNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLHQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            5081..6109
                     /gene="torT"
                     /locus_tag="ON345_002740"
     CDS             5081..6109
                     /gene="torT"
                     /locus_tag="ON345_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR3072778.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFMVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6082..6774)
                     /gene="torR"
                     /locus_tag="ON345_002741"
     CDS             complement(6082..6774)
                     /gene="torR"
                     /locus_tag="ON345_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR3072779.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6904..8076
                     /gene="torC"
                     /locus_tag="ON345_002742"
     CDS             6904..8076
                     /gene="torC"
                     /locus_tag="ON345_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR3072780.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8076..10622
                     /gene="torA"
                     /locus_tag="ON345_002743"
     CDS             8076..10622
                     /gene="torA"
                     /locus_tag="ON345_002743"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR3072781.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10619..11218
                     /gene="torD"
                     /locus_tag="ON345_002744"
     CDS             10619..11218
                     /gene="torD"
                     /locus_tag="ON345_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR3072782.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(11311..11616)
                     /gene="cbpM"
                     /locus_tag="ON345_002745"
     CDS             complement(11311..11616)
                     /gene="cbpM"
                     /locus_tag="ON345_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR3072783.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11616..12536)
                     /gene="cbpA"
                     /locus_tag="ON345_002746"
     CDS             complement(11616..12536)
                     /gene="cbpA"
                     /locus_tag="ON345_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR3072784.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3065 a         3255 c         3323 g         3037 t
ORIGIN      
        1 ttccttactg aaaatactgc gctgcactat acggggttaa taaaataaag ccagcgatat
       61 ttaagaccgc cggacggcta aaataaaatt tgcttaatct caattatcat gcgttaatag
      121 ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt actaaagcag ttctcattat
      181 caggcattat ccccttcttt tgagtctctc tcctgaacac taagtagttt ctgtattaaa
      241 gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata aaatgactgg
      301 tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg atgatggcag
      361 caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca cgctgaacga
      421 aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg cagcgaacgt
      481 tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt ccatgttgtc
      541 atggttcaca gtaccgcaca tcggcattcg atgtgacgga gcgaaaccct ttgggcgcta
      601 agtgtatttt ttgtaaatcg acgatgatca cctttgataa cgtcgcgctg caaatacgca
      661 ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg agcctttttt
      721 atatctgcct tatttttctt caatgctgta tgtatagtaa gcgataacct gttgattatt
      781 gaatctttcg gggagatggc ttataacatt tcttacctga ccagggtacc gggaaccaac
      841 accttactgg cgtgttgctg tcttttaaga ccagaagagg ttaacagtga atattgaaga
      901 gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa tcgccgagct
      961 gaacaagaat acagggaaag aagtctctga aattcgcttc accgcacgag aaaaaatgac
     1021 cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac atcaccccgt
     1081 gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct tgtggaatta
     1141 agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac attgcgtaac
     1201 caatcaccgc gaacataatc cccagaacca gtagcgtcat taaccagcca gacaacgcaa
     1261 aagctttttt atttgccgca ggtttttgca gtgaaaaggt caatgttgag gctaccggac
     1321 atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga attaatttat
     1381 ctaccgggat ccgtgatggg caattttttg cgcatttgcc acagccgata caactttcgg
     1441 cattgcgacg aatcttaaac ggcgataata gcgaaaccac gcccatcagc gcgccatatg
     1501 ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc aaaacggtca
     1561 cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcattttt acgtccatca
     1621 ccacgctgta gggcgacaac ataaaatagt gaatcgcctg agcgggcatc aataacgcga
     1681 tatagagaaa aaaactcaac agcaaatact tcacgccgcg cagaggaata tccagccagc
     1741 ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt aattcagaaa
     1801 gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga ctgatgacga
     1861 taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga cctgttgtaa
     1921 ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata cctcccggtc
     1981 tggcgacaaa tgtcgtgcta cttgccgttt cgtaatagcg cacccaatac cagaacgtga
     2041 tggcaatata aatattcatt gccaacagta ataattgcgt cgctttacgc caggtcgtgg
     2101 cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc cgctgccagc
     2161 gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac caaatgccac
     2221 tattctagtt gttcttaact ggctgatatt gattcaaatc gcgttcaggt ctttcttatt
     2281 caaccatgct tccagatcgg caacactgcg tgtaatttct tcgtgtggaa ggggggcaga
     2341 taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc ccagactcga
     2401 gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctatcct gactggcgcg
     2461 ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt gtaaataata ccagccattc
     2521 gtggatcttc tccgtcccca ttaactgagc atcttcattg agttgcgata catccagcga
     2581 ttgatcgtta ttgacttgca gttggagata gtgcgccagt aactgaccga gcacttcacg
     2641 cggcaccggt ttagggataa tcccgcggaa taatgaactg gtacgctggc gcagcgtttc
     2701 gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct gtgccagttg
     2761 tcgggcaagc gtaatgccgt cgacatccgg cagatcaaaa tccaccagtg cggcagcaaa
     2821 cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaacag caacaacctg
     2881 cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt tatcttcaat
     2941 taacagcaaa cgtaaaccgt caagacgcac cgcctgattg actgtttttg gcacgggtgc
     3001 cgtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt
     3061 ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca gtcccagccc
     3121 ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg ccagtttcgc
     3181 gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc catcagtgcg
     3241 actacgcagg ataatgtacc cttcgtcagt aaaacgcagg gcgttgctca acaggttggt
     3301 tataacctga cgaatacgtc gtggatcgcc cattaacgcg cacggcatat catcggcaat
     3361 tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt gcagggtact
     3421 ttccagcagc gggcgcggtt caaagggctc atcgctgacc gaaacattct tgccacctgc
     3481 ttcgatagcg gaataatcga gaatatcgtt gaggatggtc agcaacgatt caccagagtc
     3541 agtaattgcc cgcaaatcat cacgctgggc gttaagtgcg gggttatctg ccagcagttg
     3601 agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg ccgccagaaa
     3661 cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt gttctatcac
     3721 cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg
     3781 attcagtgca tgaacattgc tgcgaaacgc atccatcagc cgcccgatgg tatccagctc
     3841 ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca gccgttgcag
     3901 cgcctgcgtt tgttcggcaa gtggacgcgt gactgagcga taaaccacgc gccaaaggat
     3961 cagaatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt gcccgcgtgc
     4021 actggctttt tccagatgcg ccagtccgtg ctgattacgc agctcaatgg tgtcgaccag
     4081 ctgactgact tcgctactaa actgcgcgaa ctgggcgatg ttattttgtg cgagagtttg
     4141 taggtgattg ctgatttcac tgtcctgctg atacagcgcc agcaaatcgc tatattggct
     4201 aacggtagtt aacgttgttg cgacctgcgc acgaacaccc ggatcttcaa tgcgtatttg
     4261 ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg gtgcattttt
     4321 ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag cgctaaggcg
     4381 cagttcattc atctggttaa catactcaag atcgatatca atcagccgat cgagtgcact
     4441 ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct gggtcgctcc
     4501 agcggaagtt gtcgcattat tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc
     4561 agcgactatc tgctgactga gttgccgttg ttgctggcgt agttgcagac gccgccccac
     4621 cagttcccct tgctgatgta atgaacggga gatctcctgc tcctgttgtt caatagcggt
     4681 ggtatcaaaa ccttgttccc gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc
     4741 ggtgagcatt cgcccctgcg cctgccacat cttttcgtta ttggcactgg tcaggttctg
     4801 cgcggcgaaa agttcccagg cgctggcttc gctcaactgg cgcgccatat tcatggtagg
     4861 aatcaatgcc tgagtattgt ctttttccac ctggctgata aagcgcaggt tgtaccatcc
     4921 caccagggta ctggtcaggg ttaacagcgc catcagggca aagcccatcc agagtcttcg
     4981 ggtcagggtt aaattcacgg tcggtgcact ttaggtgaaa aaggttgagt cgcaaagcag
     5041 aatgcatcta gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattttt
     5101 acttctttcc cttttcatgt tgccggcatt ttcggctgat aacctgttgc gctggcatga
     5161 tgcgcagcat ttcatggtgc aagcctctat gccgcttaaa gccaaacgcg catggaaact
     5221 gtgcgcgctt tatcccagcc tgaaagattc atactggtta tcgttgaact atggtatgca
     5281 ggaggcagct cgccgttatg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca
     5341 gttggctacc cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt
     5401 actcggtagt agcacgacct catttcccga tctgcaaaag caggtagcaa gtctgccggt
     5461 gatcgaactg gtaaatgcta ttgatgctcc ccaggtgaaa agccgcgttg gtgtaccctg
     5521 gtttcagatg ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact
     5581 gaacgtgctg ttgatgcccg gacccgataa tgccgggggc agtaaagaga tggtcgaggg
     5641 ttttcgcgca gccattgccg gaagcccggt gcgtattgtt gatattgcgc ttggtgataa
     5701 cgatattgaa atccagcgta acctgttgca ggagatgctg gaacgccacc cagaaatcga
     5761 cgtcgttgcc ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa
     5821 aacgccgctt accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg
     5881 gggaagagtg attatggctg ccagcgatca aatggtctgg cagggggaac tggcggttga
     5941 gcaggccatc aggcaattac aggggcaatc ggtgtctgat aatgtcagcc caccgatttt
     6001 agttctgacg ccgaaaaatg ccgaccgtga acatattcgc cgctcgctgt caccaggggg
     6061 atttcgtccg gtctattttt atcagcacac atcagcggct aagaaataac cttcaccatg
     6121 ttgcgtcacc agtaaatccg cgctgagttt atgacgtaaa cgacgaatta acacatcgac
     6181 ggtgcgcagg tcagggtttt ccacccgacg cgcagaaagc atacgtagca gacgttcacg
     6241 gctgagaatt tcgcccggat tcgtcacaaa tgccaccaac atttcatact ctgcgcgggt
     6301 cagtttaatc ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc
     6361 ggcaaagcga tagcagttgt cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg
     6421 ccagagcaga tttttcaccc gtactaccag ttcgcgcagt tccagcggtt tggtgacgta
     6481 atcgtctgcg cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtccggtaac
     6541 cagaataatc cccaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc
     6601 atcgggtaag ttgatatcca gcagaattaa atctaccggc tgattctgca taatttcccg
     6661 cagcccggca ccgctcgctg taacggaaac ggtatacccc tcctgagtga agtaagattg
     6721 taatcgcgcc tgggtaaccg gctcatcttc aacaataaca atgtgatgtg gcatcagagg
     6781 gttttactca ttctgttcat atctgttcat attctgccgt aagccgttca tcctgaccag
     6841 tgccgctgtt catatttgct cattaagatc gcttcactaa accataattt tacaggggct
     6901 attatgcgga aactctggaa cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg
     6961 ctggtcgcaa ttgggattgt gattggcatt gcgctgattg tattgccaca cgttgggatc
     7021 aaagtcacca gcacaaccga attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa
     7081 gaatataaac agtcggtgca tttccagaac gcctccggcg tgcgagctga atgccatgac
     7141 tgtcatatcc cgccggatat tccaggcatg gtgaagcgta aactggaagc gagcaatgat
     7201 atctaccaga cctttattgc tcactccatt gatacacctg aaaaattcga agccaaacgc
     7261 gcggaacttg ccgagcgtga atgggcgcga atgaaagaaa acaactcggc aacctgccgc
     7321 tcctgccata actacgatgc gatggatcat gcgaagcagc atcctgaagc ggcacgtcag
     7381 atgaaggtgg cagcgaaaga taatcaatcc tgcatcgact gtcataaagg tattgcccac
     7441 cagttaccgg atatgagtag cggcttccgt aagcagttcg atgagctgcg cgccagtgct
     7501 aatgacagtg gtgacacgct gtactctatt gatattaagc caatttatgc ggcgaaaggc
     7561 gataaagaag cctctggttc tctgctgcct gcttcggaag tgaaagtcct taaacgtgac
     7621 ggcgactggc tgcaaattga aattaccggc tggacggaaa gcgccggacg tcagcgtgta
     7681 ctcacccaat tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag
     7741 caggtaaaaa cgctggagaa aaccaccgtt gccgacacca ataccgagtg gagcaagttg
     7801 caggccaccg catggatgaa gaaaggcgac atggtgaacg atatcaaacc gatctgggcc
     7861 tatgcggatt cgttgtacaa cggcacctgt aaccagtgcc acggcgcacc ggaaatcgcc
     7921 cactttgacg ctaacggttg gatcggcacg ctcaacggca tgattggctt taccagtctc
     7981 gataaacgtg aagaacgcac tttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca
     8041 ggtaaggctc acggcgataa gaaggaagaa aaataatgaa caataacgat ctctttcagg
     8101 catcacgtcg gcgttttctg gcacaactcg gcggcttaac cgtcgccggg atgctggggc
     8161 cgtcattgtt aacgccgcgc cgtgcaactg cggcgcaagc ggcgactgag gctgtcatct
     8221 cgaaagaggg cattcttacc gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg
     8281 gtcgctttgt ggcggcaaaa ccgttcgaac tggataaata tccgtcgaaa atgatcgccg
     8341 gattgccgga tcacgtacac aacgcggcac gtattcgtta tccgatggta cgcgtggact
     8401 ggctgcgtaa gcgccatctg agcgacacct cccagcgcgg tgataaccgt tttgtgcgcg
     8461 tgagctggga tgaagccctc gacatgttct atgaagaact ggaacgcgta cagaaaactc
     8521 acgggccgag tgccttgctg accgccagtg gttggcaatc gacggggatg ttccataacg
     8581 cttcggggat gctggcgaaa gctattgcct tgcatggtaa tagcgttggt acgggcggag
     8641 attactctac cggtgctgcg caggtgatcc tgccgcgcgt ggttggctca atggaagtgt
     8701 atgaacagca aacctcctgg ccgctggtat tgcagaacag caaaaccatt gtgctgtggg
     8761 gctccgattt gctgaaaaac cagcaagcga actggtggtg cccggatcac gatgtttatg
     8821 aatattacga gcagctaaaa gcgaaagtcg ccgccggtga agttgaggtc atcagcatcg
     8881 atccggttgt cacatccacc catgagtatc tggggcgcga gcatgtgaag cacattgcgg
     8941 ttaacccgca aactgacgtg ccgctgcaac tggcgctggc acatacgctg tacagtgaaa
     9001 acctgtacga caaaaacttc ctcgctaact actgtgtggg ttttgagcag ttcctgccgt
     9061 atctgctggg tgagaaagac ggtcagccga aagatgccgc atgggctgaa aaactgaccg
     9121 gcatcgatgc cgaaagcatt cgtgggctgg cgcggcagat ggcggcgaac agaacgcaga
     9181 ttattgctgg ctggtgcgta cagcgtatgc agcacggtga acagtgggcg tggatgattg
     9241 tggttctggc ggcgatgctg gggcaaattg gcctgccagg tggtggcttt ggttttggct
     9301 ggcactataa cggcgcaggc acgccggggc gtaaaggcgt tattctgagt ggtttctccg
     9361 gctctacgtc gattccgcct gttcacgaca acagtgacta caaaggctac agcagcacca
     9421 ttccgattgc ccgttttatc gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg
     9481 gtaaatcggt aaaactgccg ccactgaaaa tgtgtatttt tgccggaact aacccattcc
     9541 atcgccatca gcagatcaac cgcattattg aaggctggcg caagctggaa acagttatcg
     9601 ccatagataa ccagtggacc tcaacctgcc gctttgccga tatcgtgctg cctgcgacca
     9661 cgcagtttga gcgtaacgat ctcgaccagt acggcaacca ctccaaccgt ggcattatcg
     9721 ccatgaaaca ggtggtgccg ccgcagttcg aggcgcgcaa cgactttgat attttccgcg
     9781 agctgtgccg tcgctttaat cgcgaagaag cctttaccga agggctggac gaaatgggct
     9841 ggctgaaacg catctggcag gaaggtgtac aacaaggcaa aggacgcggc gttcatctgc
     9901 cagcgtttga tgacttctgg aataacaaag agtacgtcga gtttgaccat ccgcagatgt
     9961 ttgttcgcca ccaggcattc cgcgaagatc cggatctcga accgctgggc acgccgagtg
    10021 gcctgattga gatctactcg aaaaccatcg ccgatatgaa ctacgacgat tgtcaggggc
    10081 atccgatgtg gtttgagaaa atcgaacgct cccacggtgg gcccggctcg cagaagtatc
    10141 cgttgcatct gcaatctgtg catccggatt tccgacttca ctcgcagtta tgtgagtcgg
    10201 aaactctgcg tcagcaatat acggtagcgg gtaaagagcc agtgttcatt aacccgcagg
    10261 atgccagcgc gcgcggtatt cgtaacggtg atgtggtacg cgtctttaac gctcgcggtc
    10321 aggtgttggc aggggcagta gtttctgacc gctatgcacc cggcgtggca cgaattcacg
    10381 aaggggcatg gtacgatcca gataaaggcg gcgagcctgg tgcgctgtgc aaatacggta
    10441 accccaacgt gctgaccatc gacatcggta cttcgcagct ggcgcaggcg accagtgcgc
    10501 acactacgct ggtggaaatt gagaagtaca atggagcagt ggagcaggtg acggcgttta
    10561 acggccctgt ggagatggtg gcgcagtgcg aatatgttcc cgcgtcgcag gtgaaatcat
    10621 gaccacgctg acagcacaac agattgcctg tgtttacgcc tggctggcgc agttgttctc
    10681 ccgtgaactg gacgatgaac aactgacgca aatcgccagt gcgcagatgg ctgaatggtt
    10741 ttcgttgctg aaaagcgaac cgccgctggc tgcggcggtg aatgagctgg aaaactgtat
    10801 tgccacgctg acagtacgtg acgatgcccg tctggaactg gccgctgact tttgcggcct
    10861 gtttttgatg actgacaagc aggcggcgct gccgtatgca tcggcctaca aacaggacga
    10921 gcaagagatt aaacgcttgt tagttgaggc agggatggaa accagcggca atttcaacga
    10981 accggcagac catctggcga tctatctcga gttgttgagt catctgcatt tttcgcttgg
    11041 agaggggacc gttcctgcgc gaagaatcga cagtttgcgg caaaaaacac tgacggcgct
    11101 gtggcaatgg ttaccagagt ttgctgcgcg ttgccatcag tatgacagct ttggttttta
    11161 cgcggcacta agccagttat tgctggtgtt agtggagtgc gaccaccaaa acagataacg
    11221 tcgtttatgc gcctgaaaag acgcgtttag cgtcgcatca ggcattatgg cgcagttgcc
    11281 ggatgcgtca tcacatccgg caaacacaca tcacggatga gctacaaacc gggaaagccg
    11341 ctggcgtagc aggcggtttt cctgcttcag gtgtgcaata tcatccatta acgtcagcgc
    11401 caccgcgatc cccggccagt ccagagccag ttcatgacgc aggcgtaccg cgcgttgcac
    11461 cacaatggcg gcatggtcgt caaataccca ggttgtttcc tggatctcac gcggttcaac
    11521 cacccccaaa ccgacaattt cattcaactc ctcttcagag atgccggtat gcaggcaaaa
    11581 ttcggtaata gtaaaagtca ccgtaacatt agccatcatg ctttccccca atctttacgt
    11641 ggatcaaaag acgactgggc gtctgccagt tgctgccaca gcgcggcagt gttttcatcc
    11701 ggtttcggcg gcatcacgat tttcagtacc gcatacagat cgccggtctg ttttttgctc
    11761 accagacctt tgcctttaac gcgcaatcgt tgcccggcct ggctgcctgg cgggatagtc
    11821 agcaaaatgc tttctttcag tgttggaacg gtgactttag cacccagcgc cgcttcccac
    11881 gggctaaccg gcaccacaat ttccagatcc tggccgacaa tatcgaacag cggatgtggc
    11941 gcaatatgaa tcaccagcca caaatcgcca tttggaccac cgttttcgcc cggcgtcccc
    12001 tggcctttca gacggatgcg ttgaccattg ccgacgcccg ccgggatctt cacattcagc
    12061 gttttcggga tttcctgttc gatcatgcca aaggcgttat aaaccggcag gttatagctg
    12121 atggtacgct tatgctcagt cagcgtttct tcgaggaata ccgccacttc gatttcaata
    12181 tcgtggccgc gtgcggcggg gcgttgacgg ctctggcggg catgctgacc gaaaattgac
    12241 gagaagatat cgtcaaaatc ttcggcatta aaactctgac cgtcgccatg atggaactga
    12301 cggttaaatt gcggatcgtt gcgatgttgc cacatctgat catactcagc gcgacgttgt
    12361 tcatcactta acacttccca ggcttcagcg acctctttga agcgggcttc ggcatccggt
    12421 tctttgctga catcaggatg gtatttacgg gccagtcgac gataggcggt cttgattgtc
    12481 ttgagatcgt ccgtcggttt cacgcccatg atggcgtaat aatcctttaa ttccatagcg
    12541 ttatctcgcg taaatcaaca caaattgaag gaacccctgt aaggtaactc ctataagtgt
    12601 agggtaatcc tcaaaatttc atatgccaac acagaatatg ttattgaaat catcgcggag
    12661 aggaggtcgc ctcaaggtgg
//
LOCUS       DAJSKJ010000116        12545 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000116 DAJSKJ010000000
VERSION     DAJSKJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.116"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..273
                     /locus_tag="ON345_002747"
     CDS             <1..273
                     /locus_tag="ON345_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3072785.1"
                     /translation="YKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVC
                     GYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQEPTEMIQATA"
     gene            complement(313..1527)
                     /gene="ydcO"
                     /locus_tag="ON345_002748"
     CDS             complement(313..1527)
                     /gene="ydcO"
                     /locus_tag="ON345_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR3072786.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1580..2116
                     /gene="sutR"
                     /locus_tag="ON345_002749"
     CDS             1580..2116
                     /gene="sutR"
                     /locus_tag="ON345_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR3072787.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPATPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2189..4150
                     /gene="rlhA"
                     /locus_tag="ON345_002750"
     CDS             2189..4150
                     /gene="rlhA"
                     /locus_tag="ON345_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR3072788.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPAELHKICPHHPLNR
                     NLDHNWQQALIKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4242..4472)
                     /locus_tag="ON345_002751"
     CDS             complement(4242..4472)
                     /locus_tag="ON345_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR3072789.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4694..4870
                     /gene="hicA"
                     /locus_tag="ON345_002752"
     CDS             4694..4870
                     /gene="hicA"
                     /locus_tag="ON345_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HCR3072790.1"
                     /translation="MKQSEFRRWLESQGVDVANGRNHLKLRFHGRRSVMPRHSCDEIK
                     EPLRKAILKQLGLS"
     gene            4916..5332
                     /gene="hicB"
                     /locus_tag="ON345_002753"
     CDS             4916..5332
                     /gene="hicB"
                     /locus_tag="ON345_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCR3072791.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDSELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5411..6817
                     /locus_tag="ON345_002754"
     CDS             5411..6817
                     /locus_tag="ON345_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3072792.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKEPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7062..8207
                     /locus_tag="ON345_002755"
     CDS             7062..8207
                     /locus_tag="ON345_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3072793.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTKEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8225..9238
                     /locus_tag="ON345_002756"
     CDS             8225..9238
                     /locus_tag="ON345_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3072794.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9239..10180
                     /locus_tag="ON345_002757"
     CDS             9239..10180
                     /locus_tag="ON345_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3072795.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQVFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            10170..10964
                     /locus_tag="ON345_002758"
     CDS             10170..10964
                     /locus_tag="ON345_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3072796.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10986..12410
                     /gene="patD"
                     /locus_tag="ON345_002759"
     CDS             10986..12410
                     /gene="patD"
                     /locus_tag="ON345_002759"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR3072797.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEANSQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3031 a         3075 c         3362 g         3077 t
ORIGIN      
        1 tacaagcagc tctggagtgg cggtcaggtg cttgctgttc cgccagcgta tacaagccag
       61 cgttgcgcgt gctgtggtca tacagcgaaa gaaaatcgtc tgtcacaaag taaattcaga
      121 tgccaggtat gtggatatac agcgaacgcc gatgtaaacg gcgctcgtaa cattttagcg
      181 gcggggcacg ccgttcttgc ctgtggaggg atggtgcagt caggccgccc gttgaagcag
      241 gaacccaccg aaatgattca ggcgacagcc tgaacgtagc agggatccac gtccttcagg
      301 gcgtggagga tgtcaatatc ggtttctgtc agcgattaaa ttcaacacca cgtaacaaac
      361 gcctccggca attaatcccc agaacgcaga accaatcccg accagcgtca atccacttgc
      421 cgttaccaga aatgccacca ccgccgcgtc gcgctcacgc tcattatgca gcgcctgata
      481 caaactgccg ccaatggtac tcaacagcgc cagacctgcc agcatctgga tccaacttac
      541 gggcagggca gccatcatcc cggtaatggc actaccaaac agacctgcga gcaaatagaa
      601 aatgcctgca acggcagctg ccagccaacg ttgatcttta tccggatgcg cttccgggct
      661 ttggcaaata gccgcggtga ttgccgcaat accgacggaa taaacgccga aaggggaaaa
      721 aaccagtgcc agcaatccag taaatacaat taatggcgaa acaggagccg aatatccagc
      781 tgctttcatt gctgcgatac ccggtgcgtt ttgcgatgcc atcgtcacca gaaaaagggg
      841 gagtgcaacg ctcaggctgt gagcaaacga aaaatcaggg gtaatataag tggggagaac
      901 gggtttaaag acaacatcag ttgtgacaac gtcaccttgc gcgatgacga tcacgatccc
      961 aataatcatc gcggcaatta ccgcatagcg cggcgcaacg gccttggttg ccagccatac
     1021 cagcaacata cttccacaca acgtaaattg accgtccaga ctggcaaacg cctgtaaacc
     1081 aaagcgtaat aaaatcccgg caagcattgc cgccgcaagc gagtgcggaa taatgcgcat
     1141 cagacgagca aagagtcccg ttatgccgca gaggactatt aacgcgttgg tgacaataaa
     1201 aacgccgatg gcttcgttaa gtgttagtcc ctgcaatccg gtgaccaaca aagccgcgcc
     1261 aggcgttgac catgcggtga gaacaggtac gcgataccat aatgtcagag tcagcgtact
     1321 gacgcccatt gccagcccca gcgccgtcat ccagccagag atttgtgcag tggtggctcc
     1381 ggcgacaatc gctgcttgcc agattattgc cgctgaactg gcgtagccaa ttaataccgc
     1441 cagaaacccc gccagtagcg tgggtggagg aatagaaaac agacgcatag tcacctcgtg
     1501 cgttatagcg tacaggcacc gtagcatttg tccgttataa cgcacaagtg ataaacttcc
     1561 gttttgccgg aggagtcgca tggaaaatct cgctcgcttt ttatccacca cacttaaaca
     1621 actacgccag cagcgcggct ggagtctttc gcgactggca gaagcgacgg gcgtttctaa
     1681 agcaatgtta gggcagattg agcgtaatga gtccagtccg acggtcgcga cattatggaa
     1741 aattgccacc gggttgaacg taccattttc aacatttatt tctccgccgc agcccgctac
     1801 gccttcagtt tatgatccac aacagcacgc gatggtgatc acttcgctgt ttccttatga
     1861 tccacaactc tgctttgaac acttctcgat tcagatggca tctggtgcaa taagcgaatc
     1921 gacgccccat gagaaggggg taatcgaaca tgtcgtcgtc attgatggac aacttgatct
     1981 gtgcgttgat ggggaatggc agacccttaa ttgtggggaa ggcgttcgat ttgctgcaga
     2041 cgtcacgcat atctatcgta atggtgggga gcaaaccgta cattttcatt ccctcatcca
     2101 ttacccgcgc agttaagcgg gaaaactatt tcgcaacgtc gcgcttgtgg ctaaaatagc
     2161 cgccattttt cagctactgg ataagaatgt gaccgtatct tctcatcgac ttgaactgtt
     2221 aagcccggca cgcgatgccg ccattgcccg cgaagctatt ttgcacggtg ccgatgctgt
     2281 ttatatcggc ggccctggtt ttggtgcccg tcataatgcc agtaatagcc tgaaagatat
     2341 tgccgagctg gtgccgtttg cccatcgtta tggtgcaaaa attttcgtca cgcttaacac
     2401 cattttgcat gatgatgagc tggaacccgc gcaacggctg attactgatc tctaccagac
     2461 cggtgtcgat gcgctgattg ttcaggatat ggggattctg gaacttgata ttccgccgat
     2521 tgaactgcac gccagtacgc agtgcgacat tcgtacagtt gaaaaagcga agttcctctc
     2581 tgatgttggc ttcacgcaga ttgtgctggc gcgagagctg aatcttgatc agatccgcgc
     2641 gattcaccag gctacggacg cgaccattga attctttatt catggggcac tgtgcgtggc
     2701 ctattcgggt cagtgctaca tttctcatgc gcaaacaggg cgtagcgcca accgtggcga
     2761 ttgctcgcag gcgtgccgtt tgccatacac attgaaagac gatcaggggc gggtggtttc
     2821 ctatgaaaaa catctgctgt cgatgaaaga taacgatcag actgccaacc tcggcgcgct
     2881 gattgatgct ggtgtacgct ccttcaagat tgaagggcgt tacaaagata tgagctacgt
     2941 gaagaatatc accgcccatt atcgccagat gctggatgcc attattgaag aacgtggcga
     3001 tctgacgcgt gcttcatcag gtcgtactga acatttcttt gttccatcga cggaaaagac
     3061 tttccaccgt ggtagcacag attattttgt gaatgcccgt aaaggcgata ttggcgcgtt
     3121 cgattcgccg aaatttatcg gcctgccggt gggtgaagta ttgaaagtgg cgaaagatca
     3181 tctcgatgtt gccgtcaccg agccactggc aaatggtgat ggcctgaacg tgatgattaa
     3241 acgtgaagtc atcggttttc gtgccaatac ggtcgagaaa accggagaaa atcagtaccg
     3301 cgtctggccc aatgaaatgc cagcagagtt gcacaaaatt tgtccacatc acccactaaa
     3361 ccgtaatctt gatcataact ggcagcaggc actgataaaa acctccagcg aacgtcgggt
     3421 ggcggtagac attgaactgg gcggctggca ggaacaactg attctgaccc tcaccagtga
     3481 agagggtgtc agcatcacgc atacgctgga cgggcagttc gacgaagcca ataacgccga
     3541 aaaagcaatg aacaatctga aggatggtct ggcaaaactg gggcaaaccc tctattacgc
     3601 ccgcgatgtg caaattaatt tgccgggggc gctgtttgta ccaaacagtc tgttaaacca
     3661 gttccgccgt gaagctgctg acatgctgga tgctgcgcgt cttgccagtt accagcgcgg
     3721 cagccgtaaa ccggttgctg atcctgcgcc ggtttatccg caaacgcatc tgagtttcct
     3781 cgcgaacgta tacaaccaga aagcgcgtga attttatcat cgctatggtg tgcagctgat
     3841 tgacgcggcg tatgaggcac atgaagagaa gggcgaagtc ccggtgatga tcaccaagca
     3901 ttgtctgcgc tttgccttta atctgtgccc gaaacaggcg aaaggcaata tcaaaagctg
     3961 gaaggcgacg ccaatgcaac tggttaacgg cgatgaagta ttaacgctaa agtttgattg
     4021 ccgcccatgc gagatgcacg tcattggcaa aatcaaaaat cacatactga aaatgccgtt
     4081 accgggaagc gtagtggcat ccgtaagtcc ggatgagctg ctgaaaacat tgcctaagcg
     4141 aaaagggtaa aacgccagtt ttctggttac tcacaactca ttgaatctgc atgatattgc
     4201 ctgccgggta aggcgtcacg ccgcatccgg catcaaacga ctcaacgttg tcccgcttct
     4261 ggtttgcgcg atttttgcca gtaaaaatgc tcgcgcaaac cttccgccga ctcttccgcc
     4321 acagcacgca attcatcgct gtccgcttca tgacgcagct gatgatccac attctttacc
     4381 cacacaaatt catgtccttt gtgccctgcc atgagttgtc ctgagaacag cgcacacgtt
     4441 aataagacaa ccgataacgc cttcgtaaac atcctgccac ctttttctta ccttttgccg
     4501 ctatgatgcc gatcgtttct tgaggttatt attcagtttt gcaaattagc gcaaagaaat
     4561 tctggaatct tccttcctga ttttgcattg cattctgccg ttgcggcgat ttagtgctat
     4621 tttcgagcta attacacaca gaggtaaaca ctaatgttta ctttgttgat atactcagcg
     4681 gcagggaggc gatgtgaaac aaagcgagtt cagacgttgg ctcgaatctc agggcgtcga
     4741 tgtagcgaat ggccgcaacc atttgaaact caggtttcat gggaggcgca gtgtcatgcc
     4801 gcgtcactcc tgcgatgaga ttaaagaacc attgcgtaaa gcaatcctga aacaactcgg
     4861 tttgagttaa tcgccaatta aaaaggttaa tgacatgcga gggacagtcg aaattatgcg
     4921 ttatcccgtc actcttacac ccgcgccgga aggcggttat atggtttctt ttgtggatat
     4981 ccctgaagcg ttgacgcagg gcgaaactgt cgctgaagcg atggaagcgg caaaagatgc
     5041 tttactgacc gcatttgatt tttattttga agatagcgag cttatcccgt taccttcgcc
     5101 attaaatagt catgatcact ttattgaagt acctttgagc gtcgcctcta aagtattgct
     5161 gttaaatgct tttttacagt cagaaatcac tcagcaagag ttagccaggc gaattggcaa
     5221 acctaaacag gagattactc gcctatttaa tttgcaccat gcgacaaaaa tcgacgccgt
     5281 ccagcttgcg gcaaaggcgc ttggcaaaga gttatcgctg gtgatggttt aattacagtt
     5341 aacgaaaagt tgtcattttt aacaactgat atagactgcc gaatcatctg cacataatta
     5401 cgattcgata atgaaaaaat accagcagct tgcagaacaa ttacgcgagc agattgcttc
     5461 gggtatctgg caacccggcg atcgtttgcc ttcgttgcgt gaccaggtgg cgctttcagg
     5521 catgagcttt atgactgtca gccatgccta tcagttgctc gaaagtcagg gatatattat
     5581 cgcacgaccg cagtcgggtt attacgttgc gccacaggca ataaaaatgc cgaaagagcc
     5641 agtcattcca gtcactcgag atgaagcagt cgatatcaac acttatattt ttgatatgtt
     5701 gcaggccagt cgcgaaccgt cggtcgttcc gtttgcctcg gcctttcccg acccgcgact
     5761 tttccccctc caacaactaa accgctcgct ggcgcaggta agcaaaaccg ccacagcgat
     5821 gagcgtgatt gaaaacttac cgccaggaaa cgcagaactg cgtcaggcta ttgctcgtcg
     5881 ctatgcctta cagggcatca ccatttctcc tgatgaaatt gtcattactg ccggggcgtt
     5941 agaggcatta aacctcagtt tgcaagcggt aactgaaccg ggcgattggg tgatagtaga
     6001 gaatccttgt ttctacggtg cgttgcaggc gctggagcgg ctacggctga aggcgttatc
     6061 ggtggcgacg gatgttaaag aagggatcga tcttcaggcg ctggaactgg cgttgcagga
     6121 ttatccggtg aaagcgtgct ggctgatgac taatagccag aacccactcg gatttacctt
     6181 aacgccgcaa aaaaaagcac aacttgtggc gttactcaat cagtacaacg taacgctgat
     6241 tgaagatgac gtttacagcg aactttattt tggacgggaa aaaccgctgc ctgcgaaagc
     6301 gtgggatcgc cacgatggcg ttttgcattg ctcttcgttt tcgaaatgtc tggtgcctgg
     6361 ttttcgtatt ggttgggtcg ccgccggaaa acatgcacgt aaaattcaac gcttgcagtt
     6421 gatgagtacg ctttccacca gctcaccgat gcaacttgcg ctggtggatt acctttccac
     6481 gcgccgatac gacgcccatc ttcgtcgcct gcgtcgccag cttgcggaac gtaaacaacg
     6541 tgcctggcag gcactgctgc gttatctgcc tgcggaagtt aaaattcatc atagtgacag
     6601 cggttatttt ctctggctgg agctccccga gccgttagat gccggcgaat taagcctggc
     6661 ggcactgacg catcatatca gtattgcgcc gggtaaaatg ttttctaccg gtgaaaactg
     6721 gtcacgtttt tttcgtttta ataccgcgtg gcagtgggga gaacgtgaag aacaggcggt
     6781 aaaacaatta ggcaaactta ttcaagaacg gctgtaatag cgtttaattt aattcctctt
     6841 agattgggta atatgaattt cgaatagcag tcatatttcc taaccccttg tctatactcc
     6901 agaagataac cttacagacg gcataatgcg cggtagctca caacctgatt aaattttctc
     6961 aggggcgaag gtgtgcctgc aagccgccgt ctatggttaa acaaggagat atttttacgg
     7021 cacggcggct gaacaattaa ttacgacagg agtaagacct tatgagcaag acatttgccc
     7081 gcagcagcct gtgtgcgctc accatgacaa taatgaccgc tcacgccgcc gaaccaccta
     7141 ccaatttaga taaaccggaa gggcgactgg atattatcgc ctggccggga tacatcgaac
     7201 gcggacaaac tgataaacaa tacgactggg taacgcaatt tgaaaaagag acaggctgcg
     7261 cggtgaatgt gaaaaccgcc gcgacttccg atgaaatggt cagtctgatg accaaagggg
     7321 gttatgatct ggttacggca tccggcgatg cctcgctgcg tttgattatg ggcaaacgcg
     7381 tgcagccgat taataccgcc ttgattccca actggaaaac gctcgatccg cgcgtggtta
     7441 aaggcgactg gtttaatgtt ggcggcaaag tttacggcac accttaccaa tgggggccga
     7501 acctgctgat gtacaacact aaaaccttcc cgacgccgcc ggatagctgg caagtggttt
     7561 ttgttgagca aaatctgccg gacggcaaga gcaataaagg ccgcgttcag gcttatgatg
     7621 gccctatcta cattgcggac gctgcgttgt tcgttaaagc cactcagccg cagttgggca
     7681 tcagcgatcc gtatcaactc accaaagaac agtaccaggc ggtgctgaaa gtgctgcgcg
     7741 ctcaacacag tttgatccat cgctactggc atgacactac cgtgcaaatg agcgatttca
     7801 aaaacgaggg tgtggttgct tccagtgcct ggccctatca ggccaacgcc ctgaaagccg
     7861 aaggccagcc tgttgctacc gttttcccga aggagggtgt taccggttgg gctgatacca
     7921 ccatgctgca tagcgaagcg aaacatccgg tttgcgccta caaatggatg aactggtcat
     7981 taacgccaaa agtgcagggc gatgtggctg cctggtttgg ctcgttaccg gtagtgccgg
     8041 aagggtgtaa agccagtccg ttattaggcg aaaaaggttg tgaaaccaac ggttttaact
     8101 atttcgacaa aatcgccttc tggaaaacgc ctatagcaga agggggcaag tttgttccct
     8161 acagtcgctg gacgcaggat tacattgcca ttatgggcgg tcgctaactt cgctggggtg
     8221 ctttatgacg tacgcagtgg agtttgacaa cgtctcgcgg ttgtacggtg acgtgcgcgc
     8281 agtagatggc gtcagtattg cgataaaaga tggtgagttc ttctctatgc tggggccgtc
     8341 cggctccggc aaaaccacct gcctgcgcct gattgctggc ttcgaacagc tttccggcgg
     8401 ggctatctct atctttggta aacccgccag caatctgcca ccgtgggagc gggacgtgaa
     8461 tactgtcttt caggactacg cgctatttcc gcatatgtcg attcttgaca atgtcgccta
     8521 tgggctgatg gtcaaaggcg tgaataaaaa gcagcggcac gcaatggcgc aagaggcgct
     8581 ggagaaagtg gcgttggggt ttgtacatca acgtaaaccg tcacaacttt ctggtggtca
     8641 gcgccagcgg gttgctatcg ccagagcatt ggtgaatgaa ccgcgcgtat tgctgttgga
     8701 tgaaccgctc ggcgcactgg atctcaaatt gcgtgagcag atgcagctgg aactgaaaaa
     8761 actgcaacag tctctcggta tcacttttat cttcgttacc cacgatcagg gcgaagcgtt
     8821 atcgatgtcc gatcgtgtgg cggttttcaa taatggacgc attgagcagg tcgattcccc
     8881 gcgcgatctc tatatgcgcc cgcgcacgcc gtttgttgcc gggttcgttg gtacatcgaa
     8941 tgtttttgat ggactgatgg cagagaaact ttgtggcatg acgggaagct tcgccctgcg
     9001 accggaacat atccgcctca acacccctgg tgaactgcag gccaatggca cgatccaggc
     9061 ggtgcaatat cagggcgcgg caactcgttt tgaactgaaa ttgaacggcg gtgaaaaact
     9121 gcttgtgagt caggccaata tgacaggcga agaactgcct gccacgctca cgcccggaca
     9181 acaggtgatg gtttcctggt cgcgtgatgt gatggtgccg ctggttgagg agaggtgaat
     9241 ggcgatgaat gtattgcaat caccttcacg tccaggtctg ggtaaggtgt ccggtttctt
     9301 ctggcgtaat ccggggctgg ggctgttttt actgctgctt ggcccgctaa tgtggtttgg
     9361 cattgtctat ttcggctcgc tgctgacact gttatggcag gtattttata cttttgacga
     9421 tttcaccatg tcggtaacgc cggaactgac gctggcgaat atccgtgcgc tgtttaatcc
     9481 ggcgaattac gacatcattc tccgcacgct gactatggct gtggcagtca ctatcgccag
     9541 cgccattctg gcttttccaa tggcgtggta tatggcgcgc tataccagcg ggaaaatgaa
     9601 agcgtttttt tatattgcgg taatgttgcc gatgtgggcg agctacattg ttaaagccta
     9661 tgcctggacg ttattgctgg caaaagatgg cgtggctcag tggtttttac aacatcttgg
     9721 gctggaacca ctgctgactg cgttccttac attacctgcg gtgggcggaa atacgctgtc
     9781 aacttccggg ctggggcgct ttctggtgtt tctctatatc tggttgccgt tcatgatcct
     9841 gcccgttcag gcggcgcttg agcgtttgcc gccgtcattg ttgcaggcgt cggctgatct
     9901 cggcgcacgt ccacgacaaa cctttcgcta tgtggtgctg ccgctggcaa tcccgggtat
     9961 tgccgctggc tctatcttta ccttctcact cacactgggc gattttatcg tcccgcagct
    10021 ggttggtcct ccaggatatt ttatcggcaa tatggtttat tcccagcagg gggcgattgg
    10081 caatatgccg atggcggcgg cattcaccct ggtgccgatt attctcatcg cactgtacct
    10141 ggcgttcgtg aaacgtctgg gagcgttcga tgcactctga acgcgcaccg tttttcctca
    10201 aactggcggc ctggggcggc gttgttttcc tacattttcc catcctgata atcgccgcct
    10261 atgcgtttaa cactgaagat gcggcgttta gttttccacc gcagggcctg acgctgcgct
    10321 ggtttagcgt ggcagcacag cgtagtgata ttcttgatgc cgtgacactg tcacttaaag
    10381 tggcggcgct ggcgacatta attgcgctgg tgttagggac gctggcagct gccgcgctgt
    10441 ggcgacgaga ctttttcggc aaaaacgcca tttcgctgtt actgctgctg cccattgcgc
    10501 tgccgggcat tgtcactggt ctggcgttat taaccgcctt taaaaccatc aatctggagc
    10561 cgggattttt caccatcgtg gtcggtcatg cgactttttg tgtagttgtg gtgtttaaca
    10621 atgtcatcgc ccgttttcgc cgcacctcct ggagtctggt tgaggcgtca atggatcttg
    10681 gggccaatgg ctggcaaacc ttccgctacg tagtgttgcc gaatctcagt tcggcgttac
    10741 tggcaggagg aatgctggcg tttgccttgt cgttcgatga aatcatcgtt acgaccttta
    10801 cggcaggtca tgaacgaacg ttaccgttgt ggttgctcaa tcagcttggg cgaccgcgtg
    10861 atgtaccggt aactaacgtg gtggcactgc tggttatgtt ggtaacaacc ttgccgatcc
    10921 tgggggcctg gtggctaacc cgcgaaggcg acaatggtca ataaccactg atacaggaat
    10981 atgctatgca acataagtta ctgattaacg gagaactggt tagcggcgaa ggggaaaaac
    11041 agcctgtcta taatccggca acgggggacg ttttactgga aattgccgag gcatccgcag
    11101 agcaggtcga tgctgctgtg cgcgcggcag atgcagcatt tgccgaatgg gggcaaacca
    11161 cgccgaaagt gcgtgcggaa tgcctgctga aactggcgga tgttatcgaa gcaaatagtc
    11221 aggtttttgc cgaactggaa tcccgtaatt gtggcaaacc gctgcatagt gcgttcaatg
    11281 atgaaatccc ggcgattgtc gatgtttttc gctttttcgc tggtgcggcg cgctgtctga
    11341 atggtctggc ggcaggtgaa tatcttgaag gtcatacttc gatgatccgt cgcgatccgt
    11401 tgggggtcgt ggcttctatc gcaccgtgga attatccgct gatgatggcc gcgtggaaac
    11461 ttgctccggc gctggcggca gggaactgcg tagtgcttaa accatcagaa attaccccgc
    11521 tgaccgcgtt gaagttggca gagctggcga aagatatctt cccggcaggc gtgattaaca
    11581 tactgtttgg cagaggcaaa acggtgggtg atccgctgac cggtcatccc aaagtgcgga
    11641 tggtgtcgct gacgggctct atcgccaccg gcgagcacat catcagccat accgcgtcgt
    11701 ccattaagcg tactcatatg gaacttggtg gcaaagcgcc agtgattgtt tttgatgatg
    11761 cggatattga agcagtggtc gaaggtgtac gtacatttgg ctattacaat gctggacagg
    11821 attgtactgc ggcttgtcgg atctacgcgc aaaaaggcat ttacgatacg ctggtggaaa
    11881 aactgggtgc tgcggtggca acgttaaaat ctggtgcgcc ggatgacgag tctacggagc
    11941 ttggaccttt aagctcgctg gcgcatctcg aacgcgtcag caaggcagta gaagaggcga
    12001 aagcgacagg gcacatcaaa gtgatcactg gcggtgaaaa gcgcaagggt aatggctatt
    12061 actatgcgcc gacgctgctg gctggcgcat tacaggacga tgccatcgtg caaaaagagg
    12121 tatttggtcc agtagtgagt gttacgccct tcgacaacga agaacaggtg gtgaactggg
    12181 cgaatgacag ccagtacgga cttgcatctt cggtatggac gaaagatgtg ggcagggcgc
    12241 atcgcgtcag cgcacggctg caatatggtt gtacctgggt caatacccat ttcatgctgg
    12301 taagtgaaat gccgcacggt gggcagaaac tttctggtta cggcaaggat atgtcacttt
    12361 atgggctgga ggattacacc gtcgtccgcc acgtcatggt taaacattaa gttagatgtt
    12421 agtggctgtt ataggacggc cactatcgtt ttaggttctg agtttaatgg cggttaagta
    12481 ttagaatgat tacattaata actagcgtaa aaattaggct aaatgtttga tggcgatttt
    12541 tcatc
//
LOCUS       DAJSKJ010000117        12479 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000117 DAJSKJ010000000
VERSION     DAJSKJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.117"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            118..1068
                     /gene="tal"
                     /locus_tag="ON345_002760"
     CDS             118..1068
                     /gene="tal"
                     /locus_tag="ON345_002760"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR3072798.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            1088..3091
                     /gene="tkt"
                     /locus_tag="ON345_002761"
     CDS             1088..3091
                     /gene="tkt"
                     /locus_tag="ON345_002761"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3072799.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(3168..4211)
                     /locus_tag="ON345_002762"
     CDS             complement(3168..4211)
                     /locus_tag="ON345_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR3072800.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(4337..4912)
                     /gene="nudK"
                     /locus_tag="ON345_002763"
     CDS             complement(4337..4912)
                     /gene="nudK"
                     /locus_tag="ON345_002763"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR3072801.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(4980..6959)
                     /gene="aegA"
                     /locus_tag="ON345_002764"
     CDS             complement(4980..6959)
                     /gene="aegA"
                     /locus_tag="ON345_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR3072802.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVMVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            7165..8865
                     /gene="narQ"
                     /locus_tag="ON345_002765"
     CDS             7165..8865
                     /gene="narQ"
                     /locus_tag="ON345_002765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR3072803.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGKPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LILDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            9029..12142
                     /gene="acrD"
                     /locus_tag="ON345_002766"
     CDS             9029..12142
                     /gene="acrD"
                     /locus_tag="ON345_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR3072804.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(12241..12300)
                     /gene="ypfM"
                     /locus_tag="ON345_002767"
     CDS             complement(12241..12300)
                     /gene="ypfM"
                     /locus_tag="ON345_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR3072805.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         2936 a         3269 c         3297 g         2977 t
ORIGIN      
        1 cttaatccag caataccatg cctgtctgct atgctttttt gatgcgttta gcgaaatttc
       61 tcagaagtgt gaattaacgc actcatctaa cactttactt ttcaaggagt atttcctatg
      121 aacgagttag acggcatcaa acagttcacc actgtcgtgg cagacagcgg cgatattgag
      181 tccattcgcc attatcatcc ccaggatgcc accaccaatc cttcgctgtt actcaaagct
      241 gccggattat cacaatatga gcatttaata gacgatgcta tcgcctgggg taaaaaaaat
      301 ggcaagaccc aggaacaaca ggtggtcgca gcgtgtgaca aactggcagt caatttcggt
      361 gctgaaattc tcaaaatcgt acccggtcgc gtgtcaacag aagttgatgc acgcctctct
      421 tttgataaag aaaagagtat tgagaaggcg cgccatctgg tggacttgta tcagcaacaa
      481 ggcgttgaga aatcacgcat tttgatcaaa ctggcttcga cctgggaagg aattcgcgcg
      541 gccgaagagc tggaaaaaga aggtattaac tgcaacctga cgctgctgtt ttcttttgcc
      601 caggcgcggg cctgtgcaga agcgggcgtt tttctgattt cgccgtttgt cgggcgtatt
      661 tatgactggt atcaggcacg caagccgatg gacccgtatg tggtggaaga agatccgggc
      721 gttaaatcgg tgcgcaatat ctacgactac tataagcaac accactatga aaccattgtg
      781 atgggcgcga gcttccgtcg caccgaacaa atcctcgcct taaccggctg cgatcgactg
      841 actatcgcac cgaatttgct gaaggagctg caggaaaaag tttcgccagt ggtacgtaaa
      901 ttaatcccgc cttctcagac gttcccacgc ccagcaccca tgagcgaagc ggagttccgt
      961 tgggagcaca atcaggatgc gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc
     1021 gctgttgatc aacgcaaact ggaagatctt cttgccgcca aactataaac cagccacgga
     1081 gtgttatatg tcccgaaaag accttgccaa tgcgattcgc gcactcagta tggatgcggt
     1141 acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata ttgccgaagt
     1201 gctgtggaac gattttctta aacataatcc taccgaccca acctggtatg atcgcgaccg
     1261 ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctac atctgaccgg
     1321 ttacgacctg ccgctggaag aactgaagaa cttccgtcag ttgcattcga aaaccccagg
     1381 ccacccggag attggctata cgccaggcgt tgaaaccacc accggcccgc ttggacaagg
     1441 tttggcgaac gccgtcgggc tggcgatagc agagcgtaca ctggcggcgc agtttaacca
     1501 gccagaccat gagatcgtcg atcacttcac ctatgtgttt atgggcgacg gctgcctgat
     1561 ggaaggtatt tcccacgaag tctgttcgct ggcaggcacg ctgggactgg gcaagctgat
     1621 tggtttttac gatcacaacg gtatttccat cgacggtgaa acagaaggct ggtttaccga
     1681 cgatacggca aaacgttttg aagcctatca ctggcatgtg atccatgaaa tcgacggtca
     1741 cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc
     1801 gctgattatc tgccgtacgg tgattggctt tggttcgccg aataaagcag gtaaggaaga
     1861 ggcgcacggc gcaccactgg gggaagaaga agtggcgctg gcacggcaaa aactgggctg
     1921 gcaccatccg ccatttgaga tccctaaaaa gatttatcac gcctgggatg cccgtgaaaa
     1981 aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc gcctataaaa aggctcatcc
     2041 gcaactggca gaagagttta cccgacggat gagcggtggt ttaccgaagg actgggagaa
     2101 aacgactcag aaatatatca atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa
     2161 ggcttcgcaa aatacgctta acgcttacgg gccgatgctg cctgagttgc tcggcggttc
     2221 ggcggatctg gctcccagca acctgaccat ctggaaaggt tctgtttcgc tgaaggaaga
     2281 tccagcgggc aactacattc actacggggt gcgtgaattt ggcatgaccg ctatcgccaa
     2341 cggcatcgcg caccacggcg gctttgtgcc gtataccgcg acgttcctga tgtttgttga
     2401 atacgcccgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga ttatggttta
     2461 tacccacgac tcaattggcc tgggcgaaga tggtccgacg caccaggctg ttgagcaact
     2521 ggccagcctg cgcttaacgc caaatttcag cacctggcga ccgtgcgatc aggtggaagc
     2581 ggcggtgggc tggaagctgg cggttgagcg ccacaacgga ccgacggcac tgatcctctc
     2641 aaggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga ttgctcgtgg
     2701 cggttatgtg ctgaaagaca gcggcggtaa gccagatatt attctgattg ccaccggttc
     2761 agagatggaa attaccctgc aagcggcaga gaaattagca ggagaaggtc gcaatgtacg
     2821 cgtagtttcc ctgccctcaa ccgatatttt cgacgcccag gatgaggaat atcgggagtc
     2881 ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggtattg ccgattactg
     2941 gtacaagtat gttggtctga aaggggcaat tgtcgggatg acgggttacg gggaatctgc
     3001 tccggcggat aagctgttcc cgttctttgg ctttaccgcc gagaatattg tggcaaaagc
     3061 gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgctg attgccggat
     3121 gcgacgctga cgcgtcttat ccggcctaca tgtccccgca attttgttta acgggtgatc
     3181 cacaacgtgg gccaggcgtc tggcccatgc cagttatcgc aggtgggttc tgcggcataa
     3241 cgcaccaggc gaaaacgctg accgtcaaag cgccagcgcg cctgaatgcc acaatcgctt
     3301 aatccgcgcc ctttcgctaa ggtcaccagt tcacgcgatt tctcatcaaa tgttgcgttc
     3361 atcagttcca gttcattcgt ctcctgaccg ttgttgaacg gcaaacgcaa ccgaaccggg
     3421 cgcgaggcta gtggcttttt acgcgacaca atccatgcca aatcaatggt gttataggcc
     3481 cctgcctcac agctaatcat catcagcgct ttatcatcag tcagcgcagt gacattcacc
     3541 tcacgacgta acggatcaag cgagcagcgc agaccattca tccgccagtt tccataatcc
     3601 agcaaatcgt tgcgttcttc gagtgagagt ggcgtcggcg ttgggttaac caccgcgacc
     3661 tctttcagcg caggcgcggg cggtacgctg agcggcggtt cgtccccttt cttgatccac
     3721 gcggtttcac tgccaacgcg cttttgctga gcatcaataa acaacaacgc cgcttttaag
     3781 ccactcagag aaatggtctg attgccgtcg cgtaaggtga tcgccttccc ttcctgaatc
     3841 atctgcaaaa acgcggtgat ggttgccgta tcatcggtta ctaataacca tggtgaaatc
     3901 cgccacttgt caccacttaa cgccaacggc tcaccatcta acagcagccg tggcgctatc
     3961 tctccttcag acgcatccgg cgacttcaac ccgccgcgct caatacgtaa aacggcatcg
     4021 gtatgcgccc cggcgctgcg gctcagggtc atcaccagtc cattatgatc gcccgtattg
     4081 cgcgccacgc agaagttttg gttattacag gtgacctgcc agtcggaaaa cgcccgttgc
     4141 gctggtgccg cccacaccaa agacgtcggc aacaaagcga aaaaaaagag aaggaaaatg
     4201 cgatagcgca tggacggtac gaccccagaa gttcaaacaa acagcacaag tcgtatcttc
     4261 gttgcgcagg tagcgcggct caatcagatt tgtcggattg agccaataat aaacaacgaa
     4321 atattattgt tatttttcag tccattaaat gtgacgtttg caaatagtta agcaataaca
     4381 ccgtcttacc gtcacgtatc tcgccggttt tgatcatctc cagcgcctgg ctgaacggca
     4441 gctcgagcac ttcaatatct tcatcttcaa caccgccacc ggcgttagcg cgctgattgt
     4501 cactgtattc ggcgataaaa aagtggatta gctcagtcac accgccaggc gacatataca
     4561 gttcaaataa tttgcgcact tcgcccactt catagcccgt ctcttcaatc gcttctttac
     4621 gaatgcacac ttccggttcg tcgttatcca gcagcccggc gcaggtttca atcagctgcc
     4681 cgctttcatt gccattaacc caggtagcga cacggaactg acgaatcaga accacgctct
     4741 ttttcttcgc gttgtacagg aggatcgtcg caccattacc gcgatcgtaa acttcacgtt
     4801 tatggcggat aacttcgcca tctttgcgcg tgagatcgta ggtaatgttg tgcagggtga
     4861 aatagttatc ggagagaatt ttgtctttaa tgagggtgat ttgttgcgtc ataccgactc
     4921 cacagcgcga aatgaacaat tatcttacgc tgtgaagtcg ggtttgtctg cgcaggctat
     4981 cagtgagatt tgactgattt tacccccagc cagtcaataa tcccctgtgc cgcatgacgt
     5041 ccttctgcca ttgcggtaac caccagatcc gcaccacgca cggcgtcgcc accggcgaag
     5101 attttcggat tggtggtctg gtaacggtac tggctttcca catccgcgat gatgcggccc
     5161 catttgtcta ccattacacc gtgcgactcc agccacggca tcccgtgtgg attgaagcca
     5221 aacgccataa tcaccgcatc ggctggcatg acaaattcac tgccctcaat cggtacaggt
     5281 cgccgacgcc cctgggcatc cggctcgcca agacgcgtgc gcaggaaacg aatcccgcag
     5341 acgtgacctt gttcattcag ctcaagcgcc accggctgga cgttaaattc gaagttggcc
     5401 ccctcttcgc gggcgttcct cacctctttc ttcgagcctg gcatgttagc ttcatcacga
     5461 cgataagcgc aggtgacgtt actcgcgccg tggcgcagcg cagtacgcac acagtccatc
     5521 gcggtgtcgc cgccgcccag taccacgacg ttaagtccgg cggtattgat aaacggctct
     5581 tccggtagct cttcgagccc catcacctgt ttagtgttgg caatgaggaa cggcagcgcg
     5641 tcataaacgc ccggcgcatc ttcattgggt aaacccgctt tcatggaacg gtaagtgcct
     5701 acgccaacga agaccgcgtc gtattgttcc aaaagcgaat ccaaagagac atctttaccc
     5761 acttcacaat tgagttcgaa gtgaatcccc atcgcgctga agatttcccg acggcgtgcc
     5821 agcagggatt tatccagttt gaaagaagga atgccgaaag tgagcaagcc accgatttct
     5881 ggatggcgat cgtacaccgt caccgccacg ccattgcggg tcagaacatc cgcacaggcc
     5941 agccctgccg gacctgcccc gataatcgcc acccgcttgt ccactttggt gacatgactt
     6001 aagtcaggac gccaaccttt cgccaacgcc tgatctgaaa tatagcgttc aatgttgccg
     6061 atagttaccg cgccgtgctc atcgcgaata gtacaggcac cttcacacaa acggtcttgc
     6121 ggacaaacgc gtccggtaat ttccggcagg gtgttggtct ggtgagaaag ctcgacggcg
     6181 gcgtcgatgt ttccggcttt caccagttca atccactgcg gaatatggtt atgcagcggg
     6241 caggtccatt cacaaacgct gtgctcgccg cacttaaggc agcgcgaggc ttcccgttgt
     6301 gcctggtcgg cgcgaaatgg cagataaatt tcatcaaaac cggttttgcg cgcttcaatc
     6361 gccagtttat ccggctcgcc acgcgcgggc gttgcctgca tttgttcgac tttactcatt
     6421 accggcattt cttgcgccgc ggtactggca tgccacggtt gatgttcctg acgcgcggtg
     6481 cgcaagcggc gggatttcgc catgccggag agtgcgacgt cagtgaccag ttgcagcgcg
     6541 tccgccgggc aattctcaac acaggcagga ccgttttcgc gccccgcaca aaggtcgcat
     6601 ttatgcgccg tggcttttac ttttcctgcc gcgacgggtg tcaggacgat ttgcatcgta
     6661 ccaaaaggac aggccaccac gcaggattta cagccaatac acttttgctg attgacctga
     6721 atgctgtcat caacgtggct gattgcgcca ttagggcagc tacgggcgca gggcgcatct
     6781 tcacaatggt gacaggtcac tgcactacgt tgctgttgat gtttgataac cgtaattcgg
     6841 ggatgaaaat ggtgttggct caggacatgt tgctcatcat tgtgagccat gacacaggcg
     6901 atttcacaag catgacaacc cagacattgc tgactgttgg ccataataaa acgattcata
     6961 acgaccttct tttttggttg taaaaacctt attctttata tgagtgttgt tattacccga
     7021 cttacagggg aatcggcaat gttcatgtgc ccagaataag taactatttc gctataaact
     7081 gtggcagatc aaataatccc atcactgact aaattgtgtt tcaactaact ggaacattaa
     7141 tgattttttg tggagaagac gcgtgtgatt gttaaacgac ccgtctcggc cagtctggcc
     7201 cgggcctttt tttacattgt gctgctgtcg attctttcca cgggtatcgc tctgctaact
     7261 ctggcgagca gtttgcgcga cgctgaggct atcaatattg ccggatcgct gcgtatgcag
     7321 agttaccgcc tgggctacga cttgcaaagt ggcagtccac aactcaatgc acatcgccag
     7381 ttatttcagc aggcactgca ttcaccggta ttaaccaacc tcaacgtctg gtatgtgcca
     7441 gaagcagtaa aaactcgcta tgcgcatctg aatgccaact ggctggagat gaataatcgg
     7501 ctcagcaagg gcgatttgcc gtggtatcag gccaatatta ataattatgt taatcagata
     7561 gacctgttcg tgctggcttt acagcactat gctgaacgca aaatgctgct ggtggtggcg
     7621 atttccctgg ctggcggtat cggtattttc acgctggtct tttttacttt gcgccgcata
     7681 cgccatcagg tggttgcccc gctgaatcag ctggttaccg ccagtcagcg tattgaacac
     7741 gggcagttcg actcgccgcc gctggatacc aacctgccga atgagcttgg cctgcttgca
     7801 aaaaccttta accagatgtc gagcgagctg cataaattgt acctttcgct ggaggcgtca
     7861 gtagaagaaa agacccgcga tctccacgag gccaagcgtc gcctggaggt gttgtatcag
     7921 tgttcgcaag cgctgaacac cagccagatt gatgtgcatt gtttccgcca tattttgcag
     7981 attgttcgcg acaatgaagc ggctgaatat ctggagctga acgttgggga taactggcgg
     8041 attagcgaag ggaagccaaa ccctgagctg ccaatgcaga ttttaccggt gacaatgcaa
     8101 gagacggttt acggcgaact gcactggcaa aatagtcacg tttcatcatc agaaccgctg
     8161 cttaacagtg tttcatcgat gctgggacgc ggtttgtact ttaatcaggc gcagaagcat
     8221 tttcagcagt tattgttgat ggaagaacgt gcaaccatcg cccgcgaatt gcacgactcg
     8281 ctggctcagg tactttctta cttacgtatc cagttgacgt tactgaagcg ttcgataccg
     8341 gaagataacg ccaccgcaca aagtatcatg gccgattttt cccaggcgtt gaatgatgct
     8401 tatcggcagt tacgcgagct gttgactact ttccgcctga cgctgcagca ggcggatctc
     8461 ccctccgcgt tgagggaaat gctggatacg ttacaaaatc aaaccagcgc caaactgatc
     8521 ctcgactgcc gtctgccaac cctggcgctg gatgcgcaaa tgcaggtgca tttgttgcaa
     8581 attattcgcg aagcggtgct gaatgcgatg aagcacgcca acgccagcga aatcgccgtc
     8641 agttgcgtca ccgcgccgga cggcaatcac acggtttata tccgtgataa cgggattggc
     8701 atcggtgaac cgaaagagcc cgaaggtcat tatggtctga atatcatgcg cgaacgcgcg
     8761 gaacggctgg gcgggacgct gactttttca caaccttccg gcggcggcac gttagtgagt
     8821 attagctttc gctccgcgga gggtgaggaa agtcagctga tgtaatgcct cctactgacc
     8881 aaagaatact tgcacttaag gttcagtata aaagggcatg ataatttaca ttaactcctt
     8941 tttttctcca cgattggctc gtaccttgcc gctacagtga agcaagtcaa gcctacaacg
     9001 atacgcagaa acacgaggtc ctcttttaat ggcgaatttc tttattgatc gccccatttt
     9061 tgcctgggtg ctggcaatcc tgttgtgtct gacaggtacc ctggcgattt tctcattgcc
     9121 cgttgaacaa taccccgatc tcgcgccacc gaatgtgcga gtgaccgcta actatcccgg
     9181 cgcatcggcc cagacgctgg aaaacaccgt gacccaggtt atcgagcaaa atatgaccgg
     9241 cctcgataat ctcatgtata tgtcatctca gagcagtggc accggtcagg catctgtcac
     9301 tttaagtttt aaagcaggca ccgatccgga cgaagccgtg cagcaagtac aaaaccagct
     9361 gcaatcagcc atgcgaaagt taccgcaggc ggtgcaaaat cagggcgtga cggtgcgtaa
     9421 aaccggcgat accaatattc tgaccattgc tttcgtctct accgatggtt cgatggataa
     9481 acaggatatt gccgattatg ttgccagtaa tattcaggac ccgttaagcc gcgtgaatgg
     9541 cgtcggggat atcgatgcct atggttcgca atattccatg cgtatctggc tggacccggc
     9601 gaaactcaac agtttccaga tgacggctaa agatgtcact gatgccattg agtcacagaa
     9661 cgcgcagatt gcggttgggc aacttggtgg tacaccttcc gtcgataagc aggcgctcaa
     9721 cgccaccatt aacgcccagt cactgctgca aacaccagaa cagttccgcg atatcacctt
     9781 gcgggtgaat caagacggct cagaggtaag gctgggcgat gtcgccaccg tcgaaatggg
     9841 ggcggagaaa tacgattatc ttagccgctt caatggtaag ccagcctccg ggctgggggt
     9901 aaaactggcc tccggcgcaa acgaaatggc gacagcggag ctggtgctca atcgtctcga
     9961 cgagctggcg cagtacttcc cgcacggact ggaatacaag gtggcgtatg aaaccacctc
    10021 gtttgttaaa gcctccattg aagacgtggt gaaaacgctg ctggaagcta tcgccctggt
    10081 tttcctcgtt atgtatctgt tcctgcaaaa cttccgcgcc acgctgatac ccactatcgc
    10141 cgtgccggtg gtgttgatgg gaaccttctc cgtactttac gccttcggtt acagcgtcaa
    10201 caccttaacc atgttcgcga tggtactggc gatcggtctg ctggtggatg acgcaatcgt
    10261 ggtggtggaa aacgtcgaac gtattatgag tgaggaagga ctcactcctc gcgaagccac
    10321 acgtaaatcg atggggcaga tccagggggc actggtcggg attgcgatgg tgctttcggc
    10381 ggtatttgta ccaatggcct tcttcggcgg caccaccggt gccatctatc gccagttctc
    10441 tattaccatt gttgcggcga tggtgctgtc agtactggta gcgatgatcc tcactccggc
    10501 tctgtgtgcc acgctactta agccactgaa aaaaggtgag catcatgggc aaaaaggctt
    10561 ttttgcctgg tttaaccaga tgtttaaccg caacgccgaa cgctacgaaa aaggggtggc
    10621 gaaaattctc caccgtagcc tgcgctggat tgtgatttat gttctgctgc ttggcggcat
    10681 ggtgttcctg ttcctgcgtt tgccgacgtc gttcttaccg ctggaagacc gtggcatgtt
    10741 tactacctcg gtacaattgc ccagcggttc aacccaacaa cagaccctga aagtcgttga
    10801 gcaaatcgag aaatattact tcacccatga aaaagacaac atcatgtcgg tgtttgccac
    10861 cgttggttct ggccctgggg gtaacgggca aaacgtggcg cgaatgttta tccgcctgaa
    10921 agactggagc gaacgcgaca gtaagaccgg cacctcgttt gccattatcg agcgtgcaac
    10981 gaaggcgttt aaccaaatta aagaagctcg cgttatcgcc agcagcccgc cagcaattag
    11041 cggtcttggt agttctgcag gttttgatat ggagttgcag gaccacgctg gagcgggtca
    11101 cgatgcgctg atggcagcac gtaatcagtt gctggcgctg gcggcggaaa acccggagct
    11161 aacccgtgtg cgccataacg gcctcgacga cagtccgcag ttgcagattg atatcgacca
    11221 gcgtaaagct caggcgctgg gcgttgctat cgacgatatt aacgacacac tgcaaaccgc
    11281 ctggggttcg agctatgtga atgactttat ggatcgcggt cgcgtgaaga aagtctatgt
    11341 gcaggcagct gcgccgtatc gcatgctgcc agatgacatc aatctctggt atgtccgaaa
    11401 taaagatggc ggcatggtgc ccttctctgc tttcgcgacc tcacgctggg aaacaggctc
    11461 gccgcgtctg gaacgctata acggttattc tgcggttgag attgttgggg aagccgcacc
    11521 gggggtcagt accggtacgg cgatggatat tatggaatcg ttagtgaagc agttgccaaa
    11581 cggctttggt ctggagtgga cggcgatgtc gtatcaggag cgactttccg gcgcgcaggc
    11641 tccggcgctg tacgccattt ccttgctggt ggtattcctg tgtctggcgg cgttgtatga
    11701 aagctggtcg gtgccgttct cggtaatgct ggtcgtgccg ctgggggtta tcggcgcgct
    11761 gctggcaacc tggatgcgcg ggctggaaaa cgacgtttac ttccaggtgg gcctgttaac
    11821 ggtcattggt ttatcggcga aaaacgccat cctgatcgtc gagtttgcta acgagatgaa
    11881 ccaaaaaggc cacgacctgt ttgaagcgac gctccacgcc tgccgtcagc gtttacgccc
    11941 gattctgatg acctcgctgg catttatctt cggcgtattg ccaatggcaa ccagcacggg
    12001 tgccggttcc ggtggtcagc atgcggtggg tactggcgta atgggcggga tgatttcggc
    12061 cactattctg gctatttact tcgtgccgct gttctttgta ctggtgcgcc gccgcttccc
    12121 gctgaagccg cgcccggaat aagcaataaa aaaggcgaca tgctaatgtg tcgccttttt
    12181 caactttccg attaagaacc tgctcagcgg gttcttgctg tttgtacttt gtctcaggaa
    12241 ttacttacga agcataactt cgataaagtc tttccagttc cccagttcac gttcaatcat
    12301 aacaacctct cttataatta tgggtattct acggaaacaa tataccgtgg tgaagctaat
    12361 ttactcgatt gctgcgatga ctacctccgg gggataaacc ttatgtaaat actatggtcc
    12421 tgcggtgata atttgtatgt gatacacagc aacatttcga gatattcata cggcatcta
//
LOCUS       DAJSKJ010000118        12433 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000118 DAJSKJ010000000
VERSION     DAJSKJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.118"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            49..918
                     /locus_tag="ON345_002768"
     CDS             49..918
                     /locus_tag="ON345_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR3072806.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRILDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(968..1321)
                     /locus_tag="ON345_002769"
     CDS             complement(968..1321)
                     /locus_tag="ON345_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR3072807.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1459..3105)
                     /gene="groL"
                     /locus_tag="ON345_002770"
     CDS             complement(1459..3105)
                     /gene="groL"
                     /locus_tag="ON345_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR3072808.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3149..3442)
                     /locus_tag="ON345_002771"
     CDS             complement(3149..3442)
                     /locus_tag="ON345_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR3072809.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3718..4974
                     /gene="yjeH"
                     /locus_tag="ON345_002772"
     CDS             3718..4974
                     /gene="yjeH"
                     /locus_tag="ON345_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR3072810.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     TLEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4990..5466)
                     /gene="fxsA"
                     /locus_tag="ON345_002773"
     CDS             complement(4990..5466)
                     /gene="fxsA"
                     /locus_tag="ON345_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR3072811.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5803..7239
                     /gene="aspA"
                     /locus_tag="ON345_002774"
     CDS             5803..7239
                     /gene="aspA"
                     /locus_tag="ON345_002774"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR3072812.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7357..8658
                     /gene="dcuA"
                     /locus_tag="ON345_002775"
     CDS             7357..8658
                     /gene="dcuA"
                     /locus_tag="ON345_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR3072813.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8774..9112
                     /gene="cutA"
                     /locus_tag="ON345_002776"
     CDS             8774..9112
                     /gene="cutA"
                     /locus_tag="ON345_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR3072814.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9088..10785
                     /gene="dsbD"
                     /locus_tag="ON345_002777"
     CDS             9088..10785
                     /gene="dsbD"
                     /locus_tag="ON345_002777"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR3072815.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDHQP"
     gene            10822..11397
                     /locus_tag="ON345_002778"
     CDS             10822..11397
                     /locus_tag="ON345_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3072816.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPSHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11504..11579
                     /locus_tag="ON345_002779"
     tRNA            11504..11579
                     /locus_tag="ON345_002779"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11537..11539,aa:Phe,seq:gaa)
BASE COUNT         3008 a         3205 c         3034 g         3186 t
ORIGIN      
        1 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
       61 atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc actggcgctg
      121 aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgtcat ggaactgcgc
      181 gacctgctga ttgcgctgga aagtcgtctg catcaaaagc ataaactgga tgctgctgca
      241 cgccttcagt atgagcaagc tcgcgataaa gtcattaaga aaatggcaga aaacatccca
      301 gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa cacgctggaa
      361 cttaccgata atcaaggcga aaatcttacc ttcgtattaa cgctacatga cggtagcaaa
      421 tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat cattcatgca
      481 attaataatg cggaaatgcg tgaactggca ttacgtatta cctcactgtt agatttcctg
      541 ccgttatatg acgttgactg ccagcaaaat ggcaatctgg aatatgacac ctattcccaa
      601 ccggaatgga aacataactt gttcgaccat tatctggcgg tgctttaccg ctttaaagat
      661 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt
      721 aaagaaatag aggcgatcac tcgccgaata cttgatttca gcccacgact gaaaaaactc
      781 gcgggtgtac catgtcaggt ctatgttcgc accgtagcgg caaataacgc ccaaccgcta
      841 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
      901 gcaccacagg cgaaataatc tcatctgttt tactacgagg ctggaagtat ccagcctctg
      961 gatcacatca gttcggacac ttatatacct ggccgataat ctggctatcc gtcgggacaa
     1021 aactggacaa cattccctgc gacgggctac tgatgccata aatcacgtta ccgcccattg
     1081 cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt
     1141 gttgcccgga aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg
     1201 cgcctggctg ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg
     1261 agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca
     1321 tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc tgaatcttat
     1381 actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt
     1441 ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc
     1501 agcagcgcct aagtcagctg catcgttttt cggcaggtcg gtaaccatgc actcggtggt
     1561 gatcatcagg ccagccacag aagccgcgta ctgcagagca gaacgggtta ctttggttgg
     1621 atccaggata cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta
     1681 gttgccgtcg ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag
     1741 gacgatctga cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg
     1801 gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc
     1861 accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg cgtcttcaac
     1921 gcgtgctttt ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac
     1981 gccgcctgcc agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc
     2041 ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac
     2101 gccatcgatg atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc
     2161 ttccagggtt gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt
     2221 cagggttgcg atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac
     2281 tgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca gcgcttcgcc
     2341 ttctacatct tcagcgatga tcagcagcgg tttgcctgct ttggcaacgg cttccagaac
     2401 cggcagcatt tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc
     2461 cagttctact gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc
     2521 gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt
     2581 gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc
     2641 gtcggagtta gcggagatgg taccaacctg agcaatcgct ttagagtcag agcacggtac
     2701 ggacagcgct ttcagttctt caactgcagc ggtaacggct ttgtcgatac cacgtttcag
     2761 gtccatcggg ttcatgcccg cagcaacagc tttcagaccc tcagtgatga tagcctgagc
     2821 cagtacagtt gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttag aggcaacttc
     2881 tttcaccatc tgcgcaccca tgttttcgaa cttgtcttcc agttcgattt cacgagcaac
     2941 ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg
     3001 gcctttcgga ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat
     3061 tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta
     3121 aattcgtatg ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt
     3181 cggacatgat caacacttct tcattgtcga tcttctcaga tttcacaccg tagccatcgt
     3241 tgaaaataac gatgtcgcca actttcacat ccagcggctt cacttcgcca ttttcaagga
     3301 tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg
     3361 tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat
     3421 catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg
     3481 gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggattc
     3541 gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc
     3601 tgattcacct cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg
     3661 ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg
     3721 agtggactca aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta
     3781 ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac aggtagcagg caataacagc
     3841 ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt
     3901 ctgggtcgcc actatcccag cgcaggcggt gtcgcgcact tcgtcggtat ggcgtttggt
     3961 tcgcggcttg agcgagtcac cggctggttg tttttatcgg tcattcccgt gggtttgcct
     4021 gccgcgctac aaattgccgc cgggttcggc caggcaatgt ttggctggca tagctggcaa
     4081 ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt atatcggtac tcgcggtgcc
     4141 agttccagtg ctaatctaca aaccgttatt gccggactta tcgtcgcgct gattgtcgct
     4201 atctggtggg cgggcgatat caaacctgcg aatatcccct tcccggcacc tggtaatatc
     4261 gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa
     4321 gcatttgccc atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgcgcattg
     4381 atgattggtc tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac
     4441 ttcgacgcct atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtgcaa
     4501 ctgttcggtg taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc
     4561 agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat
     4621 cctgaccact acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg
     4681 gtgctcggct gctgtgtggt gagcactttg gtgattcata ctttagagat caatctggac
     4741 gctcttatta tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca
     4801 ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg gctgttatgc
     4861 gttctgttac tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggcgggg
     4921 ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc
     4981 gtttcgacat taatcctggc gatcgtcttt atgatcaagg cggtcgcgct catcatcctt
     5041 tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc
     5101 tggcatacga gaaaagcgca aatgcggcat caacttcact gtcagatgct tttgcaccgg
     5161 cggtaataaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa
     5221 accggcaatg atcagcgaaa cacttttaat catctccgcc gctgggtttt cacccgccgc
     5281 cattttttgc tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatccc
     5341 gataactgac gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac
     5401 ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca
     5461 gcgcaaaggt ttctcctgta atagcagccg gtaaaacccg gctacctgaa tgggttgcga
     5521 atcgcgttca gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt
     5581 ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc
     5641 gacacttaaa gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat
     5701 tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg
     5761 tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt
     5821 atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac
     5881 actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa
     5941 tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa
     6001 accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac
     6061 ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta
     6121 aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa
     6181 ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac
     6241 gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg
     6301 attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa
     6361 atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct
     6421 ttcagcatcc tgctgaaaga agaagtgaaa aatatccaac gtaccgctga actgctgctg
     6481 gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct
     6541 ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac
     6601 ctgatcgaag cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc
     6661 ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt
     6721 gccggcctga acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct
     6781 aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac
     6841 gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggagccg
     6901 gtcattggcc aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg
     6961 ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac
     7021 aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc
     7081 gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt
     7141 ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct
     7201 tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata
     7261 aaaaaggcac gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaacaacaat
     7321 ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga actcatcata
     7381 gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc
     7441 ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat
     7501 gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt
     7561 ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg
     7621 atcctcgcac ctatcgtgac ctatttcctg acaatctttg ctggtactgg caacatctct
     7681 ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt
     7741 ccgctgtcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg
     7801 gcagtggttt acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc
     7861 tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtcctttct ggtcactatg
     7921 ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcctg
     7981 gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg
     8041 ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg
     8101 ggtctggttg aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc
     8161 gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc
     8221 accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat
     8281 actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt
     8341 catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct
     8401 gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct
     8461 gttgcttctt tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt
     8521 gctgcggtac agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat
     8581 ccgttcttca tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg
     8641 ggtagcttca tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc
     8701 gactaacatc cttcccccgt acgttgtata gtgacctctc tcttgcggtt ccatctgttc
     8761 ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg
     8821 tacggcacca gatgaagcga cagcccagga tttagctgcc aaagtgctgg cggaaaaact
     8881 ggcggcctgc gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct
     8941 ggagcaagaa tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact
     9001 gctggaatgc ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt
     9061 tacacacgga gacacagatt acctctcatg gctcaacgca tctttacgct gatcctgcta
     9121 ctttgcagca cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc
     9181 cccgcggatc aagcctttac ttttgatttt cagcaaaacc aacatgacct gaatctgacc
     9241 tggcagatca aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac
     9301 gcgaaaattg ccgacgtgca gctgccgcaa ggcgtctggc atgaagatga gttttacggc
     9361 aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg
     9421 ggagcgacgt taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca
     9481 gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg
     9541 tctgttccgc agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg
     9601 ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct
     9661 ggcatcgtgc tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt
     9721 atttatgtgc aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca
     9781 gggttacagt tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc
     9841 tttaccttgc tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg
     9901 caaacacgtc tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt
     9961 gttatggggg cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg
    10021 attctgctgt atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc
    10081 tatgcgttgg gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg
    10141 ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca
    10201 ctgccggtct tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg
    10261 gcgctgggtg tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc
    10321 tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt
    10381 caggattggg catttggtgc gacgcatacc gcgcaaactc agacgcatct caactttaca
    10441 caaatcaaaa cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg
    10501 atgttagatc tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc
    10561 agcgacccgc aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg
    10621 gccaacgacg cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca
    10681 attctctttt ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg
    10741 gatgctgaaa ccttcagcgc acatttgcgc gatcaccaac cgtgaacgac acttgcagtg
    10801 ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta
    10861 ttagaattac aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat
    10921 ccactggacg agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg
    10981 cgttatctta gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa
    11041 accgccgaac aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac
    11101 cgctatccgg gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tagccaccct
    11161 attcatcaac tggccgatca gcaaaaaagc gcggcctacg atttcaccca cgaactgtta
    11221 accacactgg aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa
    11281 ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg
    11341 ctggcggaag atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag
    11401 aaacacagaa aagaaacgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat
    11461 taggttgacg agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc
    11521 ggtagagcag gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccac
    11581 tattcataag gcctcgctgc aaagcggggc tttttccttt tctggctacg gtagatttct
    11641 ggtcgttgca acacgttatt tgtttgctgg atttctcgcc attttcgcag atgttcgtta
    11701 cttttacaca cctcaacacc ctctgatgtg tctcttcctt accccactgc tacggtcttt
    11761 ctacggtcct ctacggttcc tttctctaag attattcata atgaagcaga gaatacgggg
    11821 gaaatggggg atttcataat ttactttatt taacaacaaa ttaccttcca ttacccccgg
    11881 gggaagaccg gggagactcc tcttcaggca agggggtgaa ctcagcgtta tcaactcccc
    11941 ttgtcagtcg ggggtatcca ccacctcctc cacctgtaat attcgcattt aaacctttca
    12001 tttcttaaca ttgcctgaat cccccgccct ccacgtcatc atagaataat ttatcagggg
    12061 tgatagtaat tgagcgaaac gtattggaac ttagacctta gggtcaaatc tgagcaaaaa
    12121 gtggaagtaa aaaatgattc tgaccactga tggcctattt caaacatcgc tgagagcgaa
    12181 tcaacaagaa gcaggccaaa gggatgaaaa ttgatccaaa tactggtgta gaaaaaaggt
    12241 atattaggtt agtttcctgg cgctctcgac cgaatatgaa ttaaaggtaa catcatagat
    12301 acacatacac cctacaactg caatgacata gccagacttg cattgactat gcacggtcac
    12361 agctatttct tctcactccg ccgccatctc aacattaact ttagccggga cttgaacgga
    12421 agcggaacac agg
//
LOCUS       DAJSKJ010000119        12248 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000119 DAJSKJ010000000
VERSION     DAJSKJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.119"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            115..1581
                     /locus_tag="ON345_002780"
     CDS             115..1581
                     /locus_tag="ON345_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3072817.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFHAYLGYLSGFAHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            1699..2685
                     /gene="yeiR"
                     /locus_tag="ON345_002781"
     CDS             1699..2685
                     /gene="yeiR"
                     /locus_tag="ON345_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR3072818.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELLASAAHSHQHVVKKGLA
                     TLSLPEHQRWRRSLNSGQGYQVCGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWIALQSALLKLRLATTA"
     gene            2724..3437
                     /gene="lpxT"
                     /locus_tag="ON345_002782"
     CDS             2724..3437
                     /gene="lpxT"
                     /locus_tag="ON345_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR3072819.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            3849..4415
                     /gene="mepS"
                     /locus_tag="ON345_002783"
     CDS             3849..4415
                     /gene="mepS"
                     /locus_tag="ON345_002783"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR3072820.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPDTRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            4596..6152
                     /locus_tag="ON345_002784"
     CDS             4596..6152
                     /locus_tag="ON345_002784"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR3072821.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTARLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIHLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            6234..8048
                     /locus_tag="ON345_002785"
     CDS             6234..8048
                     /locus_tag="ON345_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3072822.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNIAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            8049..9143
                     /locus_tag="ON345_002786"
     CDS             8049..9143
                     /locus_tag="ON345_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR3072823.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9143..10168
                     /locus_tag="ON345_002787"
     CDS             9143..10168
                     /locus_tag="ON345_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3072824.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10170..11759
                     /gene="yejF"
                     /locus_tag="ON345_002788"
     CDS             10170..11759
                     /gene="yejF"
                     /locus_tag="ON345_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR3072825.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(11763..12107)
                     /locus_tag="ON345_002789"
     CDS             complement(11763..12107)
                     /locus_tag="ON345_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072826.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         2921 a         3058 c         3060 g         3209 t
ORIGIN      
        1 cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg
       61 tcatataact ttacactgtc attgttaatt tatcgttact aagacgtgac tcctatgaag
      121 acaattgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag
      181 ttgcaatcac gtatcgttca ttttggcttt ggcgcctttc accgcgctca tcaggcgtta
      241 ctgaccgatc gtgtgctgaa tgcccagggc ggtgactggg ggatctgtga aatcagcttg
      301 ttcagcggtg atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg
      361 gagaaaggtg cggacggcaa tcaggtgata attgtcggtg ccgttcacga atgccttaat
      421 gcaaaactgg attccttagc ggcaattatt gagaaatttt gcgagccaca ggtggcaatt
      481 gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac
      541 accagtaatc cgcggattat tcacgatcta caaacccctg aagaacctca ctccgcaccg
      601 ggtattctcg tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg
      661 ctctcctgcg acaatattcc cgacaatggc catgtggtga aaaacgcggt gctgggaatg
      721 gcagaaaaac gttcgccaga actcgccggg tggataaaag aacacgtcag ttttccggga
      781 accatggtcg accgcattgt tccggctgca accgacgaat cactggcaga aatcagccag
      841 catctggggg taaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtt
      901 gaagataact tcatcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat
      961 gatgtcctgc catgggaaga gatgaaactg cgtatgctta atggcagcca ctcttttcac
     1021 gcttatctgg gttacctctc aggattcgcc catatcagtg attgcatgca ggatcgcgta
     1081 tttcgccatg ctgccagaac attaatgctg gatgagcaag cgccgacact gcaaattaaa
     1141 gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa tccgtcgctg
     1201 aaacataaga cctggcaaat cgcgatggat ggcagccaga aattaccgca acgtatgctg
     1261 gcaggtattc gcatacatct ggggcgtgaa acggactggt cgttgctggc attaggcgtt
     1321 gctggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc
     1381 gatccgctta gcgataaaat tcgcgaactt gttgcgggca gcagcagtga acaacgcgta
     1441 accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt
     1501 gtgcaggcca tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta
     1561 ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct caccttcgtc
     1621 cgctctcccc ttctcttttc tgcctttttt agccaggatt aacgctcagt taacttacca
     1681 gagtattgcg gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg
     1741 aaaaccacgt cgattcttca tctgttagcc cataaagatc ccaacgaaaa atgggcggta
     1801 ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca
     1861 ttgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta
     1921 gggttgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg
     1981 ctgggccatc cgaaacagat cctcgatctg ttaaccgcac cagtctatga accgtggata
     2041 gatctgcgcg ccaccttgtg cattctcgat ccgcgcttgc tgctggacga aaaaagcgcc
     2101 agcaatgaaa acttccgtga ccagctggct gccgcagaca tcattgtcgc caataaatcc
     2161 gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc
     2221 gatcgacaat taattcacag tgagcatggg aaagttgacg gtcatcttct ggatttgccg
     2281 cgtcgcaatt tagccgagtt gctcgccagc gccgcgcatt ctcatcagca tgtcgtgaaa
     2341 aaagggttag caacgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc
     2401 gggcaaggat atcaggtctg cggctggata ttcgacgctg atacggtatt cgacaccatt
     2461 ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt
     2521 cccgaagggc tggtacgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac
     2581 gttgcgccac cggacagccg tattgagctg atttccagca gcgaagctga ctggattgct
     2641 ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt
     2701 tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata
     2761 ttgtcggcct cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc
     2821 cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt
     2881 tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg
     2941 gaatgttgat gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga
     3001 ttatgggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa
     3061 ttcctgtaaa acgggccagc ccaacattga cttttaccga tattaaccgc gtcagcgaac
     3121 tgctctctgt tcccacgaaa gatgcctcac gagatagctt tcccggcgat cacggcatga
     3181 tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg
     3241 cccttattat ttttgtggtt tttgcatttc ccagagtaat gattggcgca cactggttta
     3301 ctgacatcat tgtcggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga
     3361 cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccggga aaaaacaaac
     3421 atttccaaaa caaataactc acagtaatta acatcatcag gcttattttt atagtgagga
     3481 taatcctgat gatgcgcacc gtgctttcat ccatcgcgcg caaaaatcat tctctaagta
     3541 aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa ttgaccaatc ccgcttatta
     3601 actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc
     3661 aatcgggtaa tctcgaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt
     3721 ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag
     3781 tctcgtgtcg cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa
     3841 caaacaacat ggtcaaatct caaccgattt tgagatatat cttgcgcggg atccccgcga
     3901 ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc
     3961 ctgatacacg tgcagtgggt agtgaaacat catcactgca agcttctcag gatgaatttg
     4021 aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga
     4081 aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt tctggttttg
     4141 tacaacgtac attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc
     4201 aggaaatggg taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc
     4261 gtgccggttc aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg
     4321 cttccaccag cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt
     4381 acaacgaagc acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg
     4441 gctatcctga cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat
     4501 cagagaaaat gatgttgccg cgtcttgatc caggctatag tccggtcatt gttatctttt
     4561 aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg
     4621 gacgtaagct cctgcttacc tgcattgtag caggcgtgat gattgcgata ctggttagct
     4681 gccttcagtt tttagtggcc tggcataagc acgaagtcaa atacgacaca ctgattaccg
     4741 acgtacaaaa gtatctcgat acctattttg ccgacctgaa atccactact gaccggcttc
     4801 agccgctgac cttagatacc tgccagcagg ctaaccccga actgaccgcc cgcgcggcgt
     4861 ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc tgttcatctg
     4921 cgaccggtga gatggacatt ccactcaatg aattgattcc tgcgctcgac attaataaaa
     4981 atgtcgatat ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca
     5041 tctggtatcg caaccctttg ctgaaaaata gcggcgtctt tgccgctctg aatctcaacc
     5101 tgacgccttc tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta
     5161 ttggcaatac tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg
     5221 acatgccggt ccgtgaaact aaaattgcgg gcattcctct gaccgctcgg ctttatgcgg
     5281 atgactggac atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg
     5341 tcgttggcct gctctgctat tacctgatga gcgtacgtat gcgccccggc agagaaatca
     5401 tgaccgccat caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaag
     5461 ctttgcgagt aacgggcctg gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa
     5521 ttcccccgga tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccgctgactc
     5581 agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt agaaaaagtg ctgccagtag
     5641 gcgtcaaatt tggtattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag
     5701 atatccagaa actgctcact tccctgcccg cacaccattt ccagattgtg ctggaaatta
     5761 ccgagcgcga tatgctgaaa gagcaagaag ccacacaact cttcgcctgg ctgcactcgg
     5821 tcggcgtaga aattgctatt gatgacttcg gcaccgggca cagcgcgctt atccatcttg
     5881 agcgttttac gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg
     5941 aaacgatcac ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgc ctcaatatgc
     6001 tgacggttgc tgaaggggtc gaaacgccgg aacaggcgcg atggctaagc gaacgcggcg
     6061 ttaatttcat gcaaggctac tggattagtc gccctttacc gctggacgat tttgtccgct
     6121 ggctgaagaa accgtatacg cctcagtggt aaggtgtgct tacgtccctt attattcata
     6181 gtgaaagcat gccggattgc ggctaatgat gagtaaaagg aaatccgttg cagatgattg
     6241 tgcgcatact gctgctgttt atcgctctgt tcacctttgg tgtgcaggcg caggctatca
     6301 aggaaagcta tgcctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt
     6361 ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca gccctcggca
     6421 ccttcgataa tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc
     6481 tgtacgacac gctatttacg acttccgatg acgaaccagg cagttattac ccgctgattg
     6541 ctgaaagcgc acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgcgcg
     6601 cccgttttca tgatggttcg cccattactg cccgcgatgt agagtttact tttcaaaaat
     6661 ttatgaccga aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca
     6721 ttgcgccgtt aaccgtgcgc attgagttag ctaaacccgg caaagaagat atgttgagtc
     6781 tgttttcgct gccggtattt ccagaaaagt actggaagga tcacaaactt agcgacccgc
     6841 tcgccacgcc tccgcttgcc agtggtccgt accgcattac gtcctggaaa atggggcaaa
     6901 atattgtcta ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgcggac
     6961 gctggaattt cgacaccatt cgctacgatt attacctcga tgataatatc gcctttgaag
     7021 cgtttaaagc aggtgccttt gatttgcgta tggaaaacga cgccaaaaac tgggccacgc
     7081 gttataccgg taaaaatttc gataaaaaat acatcatcaa agatgagcaa aagaacgaat
     7141 cagcccagga tacgcgctgg ctggcgttta atatccaacg tccagtattc agcgatcgcc
     7201 gggtgcggga agcaatcact ctcgcctttg actttgaatg gatgaacaag gcgttgtttt
     7261 acaatgcctg gagtcgcacg aacagttatt ttcagaatac cgaatacgcg gccagaaatt
     7321 accccgacgc cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag
     7381 tcttcacaca aatctaccag ccgccggtat ccaaaggcga tggctacgat cgtgacaacc
     7441 tgttaaaagc cgacaaactt ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg
     7501 ttaatgccac aacgggtcag ccactcagct ttgaattatt gcttcccgca agcagcaata
     7561 gtcagtgggt attgccgttc cagcacagcc tgcaacggct gggtatcaac atggacattc
     7621 gcaaggtgga taactcgcaa atcactaacc gcatgcgcag tcgcgactat gacatgatgc
     7681 cgcgcgtatg gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg
     7741 aatatatcaa ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcgctga
     7801 tcaaccaaat tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac
     7861 tggatcgcgt attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc
     7921 gtctcgcctg gtgggataaa ttctcccagc cggccgtgcg ccccatctat agcctcggta
     7981 tcgatacctg gtggtatgac gtcaataaag cggccaaact gccgtccgcc agcaaacagg
     8041 gagagtagat gggcgcttac ctgattcgcc gtctgttgct ggtgatccca acattatggg
     8101 cgattatcac catcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgaccagg
     8161 ccatcgccgc cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg
     8221 ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaat taccgtggcg
     8281 gacgcggatt agatccagaa gtgatcgctg agatcactca tcgctacggt tttgataagc
     8341 cgatccacga acgttacttc aaaatgctct gggactacat ccgctttgat tttggcgata
     8401 gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg gtttccatca
     8461 ccctcggatt gtggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca
     8521 aagctgttta taatgggagc cgctttgacg tctggagtag cgcatttatc atcatcggct
     8581 acgccattcc ggcctttttg tttgccatcc tgctgattgt cttcttcgcg ggcggcagct
     8641 atttcgacct gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt
     8701 atcagaaaat caccgattat ctgtggcata tcacgctgcc ggtgctggcg acagtgattg
     8761 gtggctttgc ggcgctgacc atgctgacaa aaaactcatt ccttgatgaa gtgcgcaagc
     8821 aatacgtggt gaccgcgcgt gcgaaagggg taagtgaaaa aaatattctc tggaaacatg
     8881 tgttccgcaa cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt
     8941 tttttaccgg ctcgctgctg attgaggtga tgttttcact caatggtctg ggcttactgg
     9001 gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca
     9061 ccctgattgg cctgctgctg aatattgtca gtgatatcag ctatacgctg gttgatccgc
     9121 gtattgattt tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc
     9181 gcgttttcgc cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg
     9241 tttgagtttg tgttctgaac ttatcgccaa cgataaaccg ttgctggtgc gttatgacgg
     9301 cagttggtat ttcccgttat tgaaaaacta cagcgaaagc gattttggcg gcccgctggc
     9361 aagtcaggct gattatcagg acccgtggct gaaacaacgg ctggaaaata acggctgggt
     9421 actgtgggca ccgattcgct ttggtgctac cagtatcaac tttgctaccg ataagccctt
     9481 cccttctcca ccctcccggc aaaactggct gggaacggat gccaacggcg gcgatgtgct
     9541 ggcacgcatt ctctatggca cgcggatctc ggttctgttt ggcctgatgc tgactctctg
     9601 ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga
     9661 tctctgggga caacgcttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat
     9721 tttactttcc agcgtcgtac agcctaactt ctggtggctg ctggcaatta ctgtcttgtt
     9781 tggctggatg agtctggtcg gcgtggtgcg ggcggagttt ttacgtactc gtaatttcga
     9841 ctacattcgt gcggcacagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat
     9901 gttgcctaat gccatggtcg cgaccctcac ctttttaccg tttattttat gtagttcgat
     9961 aaccaccctg acctcactcg atttcctcgg cttcggtctg ccgctcggtt caccgtcact
    10021 cggtgaactg ctgttacaag ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc
    10081 cttcttgtcg gtggcgatat tattgtcttt gctgatcttt attggtgaag ccgtccgcga
    10141 cgcatttgat cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaaaatt
    10201 tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcactac
    10261 agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta
    10321 ccgcgctgtc aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata
    10381 ttcgttttca tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg
    10441 gtaataagat cgccatgatt tttcaggaac cgatggtgtc gttaaatcca ttgcataccc
    10501 tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc
    10561 gtggcgaaat tcttaactgc cttgatcgcg ttggtatccg ccaggcggca aaacggctga
    10621 cagattatcc gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc
    10681 tgttaacgcg accggaatta ttaattgccg atgaaccgac caccgcactg gacgtctctg
    10741 tccaggcgca gattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc
    10801 tgtttattac tcataacctc agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc
    10861 aaaacggtcg ctgtgtcgag caaaattacg ccgctacgct atttgcctca cccactcatc
    10921 cttacacaca aaagctactc aacagtgaac cgtcaggcga cccggtgccg ttgccagaac
    10981 ctgcctcaac gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga
    11041 ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacgag
    11101 cgggtgaaac actgggttta gtgggcgagt ccggttccgg gaaaagtacg acgggactgg
    11161 cgctgctgcg actgattaat tctcagggca gcatcatctt tgacggtcag ccactgcaaa
    11221 atttaaatcg ccgccagctg ttacctattc gtcatcgcat tcaggtggta tttcaggatc
    11281 caaactcctc gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg
    11341 ttcaccagcc gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg
    11401 aagtgggatt agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc
    11461 gacaacgtat tgcgattgcc agggcattaa ttcttaagcc ctcgctgatc atacttgatg
    11521 aaccgacatc atcactcgac aaaacggtac aggcgcaaat attgacgcta ttgaaatcat
    11581 tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca cgatttgcac gttgtccgcg
    11641 cgttatgtca tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg
    11701 cgcgcgtgtt tgccacaccg cagcaggagt atacgcgtca gctactggcg ttgagctgac
    11761 gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa atttttcaat
    11821 cggcatgttg cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac
    11881 tccagcaccg aacatggaac ttcgatgtcg cttaacgcct ggcttagttt atacatcacc
    11941 gaccatgcat tatcaccatc cgggctaagc aatttaagcg ccaccaggct gttgtcaccg
    12001 cacggtccat tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga
    12061 tagttctgcg gcagtcgatg gacgattgag agttgtaatg aagtcactta ttttccccgg
    12121 aagcacattc acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct
    12181 ttatacagat cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaagt
    12241 aatatctg
//
LOCUS       DAJSKJ010000120        12109 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000120 DAJSKJ010000000
VERSION     DAJSKJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.120"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(75..983)
                     /gene="galU"
                     /locus_tag="ON345_002790"
     CDS             complement(75..983)
                     /gene="galU"
                     /locus_tag="ON345_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3072827.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1185..2198)
                     /gene="rssB"
                     /locus_tag="ON345_002791"
     CDS             complement(1185..2198)
                     /gene="rssB"
                     /locus_tag="ON345_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR3072828.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2290..3195)
                     /gene="rssA"
                     /locus_tag="ON345_002792"
     CDS             complement(2290..3195)
                     /gene="rssA"
                     /locus_tag="ON345_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR3072829.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARRKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAVGQLAVERKMDELLPLVRTNI"
     gene            3308..3766
                     /locus_tag="ON345_002793"
     CDS             3308..3766
                     /locus_tag="ON345_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR3072830.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3816..4658
                     /gene="purU"
                     /locus_tag="ON345_002794"
     CDS             3816..4658
                     /gene="purU"
                     /locus_tag="ON345_002794"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR3072831.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4818..4902
                     /locus_tag="ON345_002795"
     tRNA            4818..4902
                     /locus_tag="ON345_002795"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4852..4854,aa:Tyr,seq:gta)
     gene            4950..5081
                     /locus_tag="ON345_002796"
     ncRNA           4950..5081
                     /ncRNA_class="other"
                     /locus_tag="ON345_002796"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5265..5942)
                     /gene="narI"
                     /locus_tag="ON345_002797"
     CDS             complement(5265..5942)
                     /gene="narI"
                     /locus_tag="ON345_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR3072832.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5942..6652)
                     /gene="narJ"
                     /locus_tag="ON345_002798"
     CDS             complement(5942..6652)
                     /gene="narJ"
                     /locus_tag="ON345_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR3072833.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6649..8187)
                     /gene="narH"
                     /locus_tag="ON345_002799"
     CDS             complement(6649..8187)
                     /gene="narH"
                     /locus_tag="ON345_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR3072834.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8184..11927)
                     /locus_tag="ON345_002800"
     CDS             complement(8184..11927)
                     /locus_tag="ON345_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR3072835.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPKWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2885 a         3282 c         2974 g         2968 t
ORIGIN      
        1 tttaataaga ataagctata aaaaaacggc gtcgattgct caacgccgtt tcgtggataa
       61 caccgatacg gatgttactt cttaatgccc atctcttctt caagccaggc tttaaattcc
      121 gtgccaaggg tgttatgacg aataccgtat tcaacgaagg cctgcatgta acctaattta
      181 ttaccgcagt catggctctt ccctttcata tgataggctt ccaccgtttc tttttcgatc
      241 agcatatcaa ttgcgtcggt gagctgaatt tcatcaccag ctcccggagg ggtttttgcc
      301 agcaacggcc aaatatccgc gctaagtacg taacgaccca caatagcgag attagacggc
      361 gcaacatccg ctttcggttt ttctaccaca ccaaccatcg gtacgctttc acccggcgct
      421 aattcaacgc ctttgcaatc cacaacgcca tatgcggtca catcagcaac cggttcaacc
      481 atgatctggc tatgacccgt ttcatcaaag cggcggatca tctctgccag gttatcctgt
      541 gacaaatcgg attcatattc atccaggata acatcaggca aaataacagc taccggttca
      601 tcacccacta ccgggtgagc acacaatacc gcgtgtccca ggcctttcgc cagaccctga
      661 cgaacttgca taatagtcac gtgcggtgga caaatagact gcacttcatc aagcagttga
      721 cgttttacac gtttttccag cattgcttcc agttcaaaac tggtatcaaa gtggttttca
      781 atagagtttt tagatgagtg tgtaaccagc acaatttcag taatgccagc cgcaatacat
      841 tcattcacga cgtattgaat taatggctta tcgacaagtg gcagcatctc tttcgggatg
      901 gctttcgtcg ccggcaacat cctggttcct aatcccgcaa cggggataac ggcttttttg
      961 actttcgtat taatggcagc catttaaatt ctcctagact gttcgtgttt tgaacgtgtt
     1021 catatttctg tatcgcatcc cagtatatca gcaccccgaa ggggctaagt tctgaaaagg
     1081 aggcgagtta gcataattaa gacaattact tatacgaatg gcgacgatag taccaccagc
     1141 cgacattagc aggtaatgca aatttagccc gcgttatcgt ttgctcattc tgcagacaac
     1201 atcaagcgca gtcgaccacc ggttccccat atttggcatt gccaggcatc gcatcgctgg
     1261 ctcaattgat tcaaataagc gttacctaaa gtgcctaacg gaacaccatt actgatttgc
     1321 acctggtgtt cgccggtatt taacgtcgca ttcagacccg cagaaaccag aatgagattt
     1381 ttcagttcgc gatgataata gccaactaat agcggaaact gccccggcag attggcctga
     1441 cgaagtaaat ggtttacctg cttcaataac gcgcccaact ctggcaaccg ttgattttgg
     1501 tgtgcaagct gttcctgtaa taatccgtta aacaatgcgc gtaataacaa ggcagcaagt
     1561 acgccattat gtccagctcg ggtgacatca aggcaataaa atgccagatc gttttccgaa
     1621 agtgcggcaa tatcaagcac caggccgggt ttgtccgcgg caaccaattg acgataatta
     1681 acccggcaat gggaaatcac ctgctgaacc ggcggttgta gttcctgtaa taatttcgcc
     1741 gctgcggcag ggttatcaac cattgcatcc cagtcgcgaa aaagcctttc ctcttcctca
     1801 acgcgcgaat taaacatgct gggatagaga caggcaaaaa ccatctcgcg caagcgattc
     1861 agatctttaa ctggtttcag caaaacatct tcaacgccca gacgtaacgc tttggcaata
     1921 tctgccatat tttcagtggc agatatcacc agaactgggg tctggtcgcc tctgttacgt
     1981 atatgctcca gcagtttaag cccgttcatt cgtggcatcg cgatatcaca tatcatcagg
     2041 tctggagtga aacctcccag caactcaagg gcatccaccc catcagccgc cagtaccgtt
     2101 gtcgctccca atgaggaaaa ccatgaatcc agaagcgagc gaaatacctg ctcatcttca
     2161 acaatgagaa tctgttttcc gaccaatggc tgcgtcatgt tctctcccct gactggcttt
     2221 actcaatagt ggcatgctat tgccacctgc gcctgtcaga attaacttaa gtgtagataa
     2281 aaattctggt caaatgttgg tgcgtaccaa cggcaaaagt tcgtccattt tcctttccac
     2341 tgccagctgt ccgaccgcaa tggcagcgtg cgcgcgatgg aaatcaagcg tagatatttg
     2401 cgggcaaaca ggttgaatca gaatatcggg cggatcacct gccatgcggt tccttttaag
     2461 gcggttctcc agcacctgga tagaagtggt cataatctct gttgccgttg gcgctgtcac
     2521 cgcccgacgt gtcgttatgc tgcctaacct ttctttccga cgcgcatgcc acggcagaga
     2581 atcaccattc tcgctatttt cttcactgac attaaaggag agcaaatctt gttgcatcaa
     2641 atgagcatcg tgctgcaggt caaccgctat cacaatatca gcccccaatg cacgcgtgag
     2701 ggaaatagga attgggttaa cgactgctcc atcaaccagc cagtagccgt tatgtgcaac
     2761 aggtgccatg agtcctggaa tactgcatga tgcgcgaata gcaagatgga gatcgccttc
     2821 agtaaaccat aattcacgtc ccgtacttaa attggtggca acagccgcaa agcgacggga
     2881 acaattttcg atctctgttt ccggcattat ttcgcggtat tgattgaaga cacgctcgcc
     2941 gcgcagtaac ccaccgcgct gccaggagag atccatcagg cgtaaaacgt cccaataact
     3001 gaaagaggtt acccaatctt ccagcgcaga taatcgatcg catgcatagg ccgcgcccac
     3061 cagcgaacca attgaacatc ctgcaacgat atcaatttca atacccactt tttttagcgc
     3121 attaataacg ccaatatgcg accaacctct cgccgcgcca gatcccagcg ccagccctat
     3181 ttttatcttt ctcattattc ccgcaagatt cccctggaat gcaatcgtag ccacatcgct
     3241 actgctcggt taacatagtg ctactcaggc ggttaatacg ccgttattgt ttccagggag
     3301 attatttgtg tctcagcttt gtccctgtgg tagtgctgtc gagtatagcc tatgttgcca
     3361 cccttatgtg tctggtgaaa aggttgcacc tgatccagaa catctcatgc gttcgcgtta
     3421 ctgcgctttt gtgatgcaag acgcagatta tttaataaag acctggcatc cctcttgtgg
     3481 agcagcggcg ttacgtgccg aattgatggc cggatttgcg catactgaat ggttgggatt
     3541 gaccgtattt gaacattgtt ggcaggacgc cgacaatatc ggatttgtca gttttgttgc
     3601 tcgatttacc gaaggtggca aaacaggtgc aataattgaa cgttcgcgat ttttgaaaga
     3661 aaacggtcaa tggtactata ttgacggtac acgtccgcag tttggtcgca acgatccctg
     3721 cccttgtggt tcaggtaaaa aatttaaaaa gtgctgcggc caataatggt tgacggtacg
     3781 gtttagcaaa cactctcaac aaggtttttc cagcaatgca ttcactccaa cgtaaagttc
     3841 tgcgtactat ttgtccggac caaaaaggtc tgatcgcacg tattaccaat atttgctaca
     3901 agcacgagtt aaatatcgta cagaacaatg aatttgttga tcaccgtacc gggcgctttt
     3961 ttatgcgcac ggaactggaa gggattttta atgattccac ccttctggcg gatctcgata
     4021 gcgcattgcc agaaggctcc gtgcgtgagc tgaatcctgc cggtcgtcgc cggatagtga
     4081 ttctggtcac taaagaagcg cattgccttg gcgatttgtt gatgaaagcc aattacggcg
     4141 gcctggatgt cgaaatcgcg gcagttattg gtaaccacga tactttacgt tctctggttg
     4201 agcgttttga tattccgttt gagctggtaa gccatgaagg gttaagccgc aacgagcacg
     4261 atcaaaagat ggcggacgcc attgatgctt atcaacctga ctatgtggtg ctggcgaagt
     4321 atatgcgggt attaacgccg gaatttgtgg cacgcttccc gaataaaatc atcaatattc
     4381 accattcatt cctgcctgcg tttatcggcg cacgtcctta tcaccaggcc tatgaacgtg
     4441 gtgtgaagat tattggcgca accgctcact atgtgaatga caatctggac gaaggcccaa
     4501 tcatcatgca ggacgttatt catgtcgatc atacctacac agctgaagat atgatgcgcg
     4561 caggtcgtga cgtcgagaaa aacgtcttaa gtcgtgcgct atacaaagta ctggcacagc
     4621 gcgtctttgt ttacggtaat cggacgatta ttctttaatc gccagcaaaa ataactggtt
     4681 acctttaatc cgttacggat gaaaattacg caaccagttc atttttctca acgtaacact
     4741 ttacagcggc gcgtcatttg atatgatgcg ccccgcttcc tgataaggga gcaggccagt
     4801 aaaaagcatt accccgtggt ggggttcccg agcggccaaa gggagcagac tgtaaatctg
     4861 ccgtcatcga cttcgaaggt tcgaatcctt cccccaccac catcactttt caaaagtccg
     4921 taaactctca aacgaatccg caattaaata ttctgcccaa gcggggaagg atgagaagct
     4981 tcgaccaagg ttcgacttga gcgccagcga gagagcgttg ccgcaggcaa cgacccgaag
     5041 ggcgaagcgc gcagcgctga gtaatccttc ccccaccacc atcgcttcct taaataatca
     5101 aaacctgtta ctccgagcta attttgcccg atggcaccac gtttaccggg cctacaatat
     5161 cctcccctgt aggtcggata agcgaagcgc atccgacaac tcaccccgca ccgtgcccat
     5221 aaaaaaaccc cgccgaagca gggtctatgt gaactaaaat tcgcttagtg acgagcgcgc
     5281 actagctggt acttacgtgt cagatactcc accggtacgc tccagatgtg taccagacgc
     5341 gagaacggga acagcaggaa taacgtcatc cccagcacta ggtgcagacg gaagataaac
     5401 gccacaccat cgaggtgttg agaagcgcca ccgtggaagg tcaccaccga ctgcgcccag
     5461 ccaaccagtt tcatcatctc gctaccgtcc atatgctgag cggagaacgg aatggtcaac
     5521 aggcccagcg cgcactggat aacgagcagc gacaggatca ggatatccgc tccggtagtg
     5581 gttgcacgca cgcgtgggct gaacagacga cgcttcagca acagcacgcc gccaatcaga
     5641 cacagcacac cgctggcacc accagcaaac atcgccattt tctgtttcac ttcaatcggc
     5701 agccaggctt catacatcca gtgcggcgtc agcataccga agaagtgacc aacaaaaatc
     5761 cccagaatac cgatatggaa caggttcgac gccaggttca tccctttgcg atccagcatc
     5821 tggctggacg ccgcgcgcca ggtgtactgc ccgtagtcat aacgcagcca gctaccaatc
     5881 aggaagaccg ccccggctat gtacgggtag atatcaaaga agaacatatt caggaattgc
     5941 attagtgctg tcctccggtg gtgatattca gatattgcgg cgcgacggca ccggcaaagc
     6001 gacgctgatg agcagtgatt gctgaatcac cgcagccttt gtcagcaaag aatttaacct
     6061 gctcttcttc ccagacagca tccagcgcct gcggcgtatc atcgcgcgct tcgtcggcaa
     6121 ttttttccgc cactttgtcg ctgtcgatag cggtattggc caatttcagc agcagatcaa
     6181 acatcacggc ataacggctt tcacgctgtt gcagacgcgc gctcagcaat gccagaatcg
     6241 gcgcgatatc tttcaaacct tccacggctt cgctttgcgg cagctgcgac aggtactcca
     6301 gatacagcgg cagatggtcc ggtaattcgc ggctgtttaa ctgcaagccg tgctgctcgt
     6361 actgcgccag caggtccacc atcgcctggc cgcggtcacg ggattcaccg tgaacatgtt
     6421 caaatagcaa cagtgacgtg gcgcggccac ggtcgaacag ttcgctgtac tgggcctggg
     6481 catcgagcgg atccatcgcc gttaaatcgc gcaggaaaat gcccagcgca tgggcatctt
     6541 ctttcgacag atttttcgac gcagcaatcg cctcaaacat ctcttgttga tgctgccata
     6601 aggcagcatc cggatattca aggagacgcg atacaatcac gagttcgatc atggatgcgg
     6661 ctccgttttg ctggtcacat cgatggcatc gatacgacgg ctgttgaaca gattgaattt
     6721 ggtatctgaa ccgtggcagc catcaccaaa ggtaaagccg cagccatttt tctccgggaa
     6781 ggcttcccgt gccagttcac gatgactact cggcaccaca aagcgatctt cgtagttagc
     6841 aatcgccaga taacggtaca tctcctgtgc ctgggcttcg gtcagaccga cctcttccag
     6901 cgcacgggta tcaactttac cgtcaacggt ttcagcacgt ttgtaatgac gcatcgccag
     6961 catacgtttc agtgcgcgca gtaccggttt ggtatcaccg gcggtcagca gattcgccag
     7021 atactgtacc ggaatacgca agctttcgac gtctggcaga atgccgttgc tacccagctc
     7081 acccgcgtct gctgcagact gaatcggaga cagaggcggc acgtaccaga ccatcggcag
     7141 tgtgcgatat tctggatgca gcggcagcgc cagtttccat tccattgcca ttttataaac
     7201 cggcgactgc tgtgcggctt caataacgct cagcggaata ccgtctttaa tcgcctgctc
     7261 gatgactttc ggatcgttcg gatcgaggaa cacctccagc tgacgctggt aaagatcttt
     7321 ctcgttctcg gtgctggctg cacgttcaat cgcgtcggca tcgtacagca gcacgccaag
     7381 ataacggata cgaccgacac aggtttctga gcacacggtc ggctgacccg cttcaatacg
     7441 cggatagcag aagatgcact tctcagactt accgctcttc cagttgaagt agattttttt
     7501 gtacgggcat ccggtgatgc acatacgcca gccacggcat ttatcctggt cgatcaggac
     7561 gatgccatct tcttcacgct tgtaaatcgc accgctcggg caggtcgcca cacatgccgg
     7621 gttcaggcag tgttcgcaca ggcgcggcaa atacatcata aaggtgtttt cgaactggct
     7681 atacatcgcc ttctggatgt tgtcgaagtt cttgtctttc gccagtttgt caaactcacc
     7741 acccaggtca tcttcccagt tcggcccttt ttcgattttc gccatccgtt ccccggtaat
     7801 cagcgaacgc ggacgggcaa tcggctgcga tttgctgcct tccggcgcgg tatgcaggtt
     7861 ctgatagtca aaatcgaacg gctcgtaata atcgtcgatc cccggcagat gcgggttagc
     7921 gaagatttta cccagcaaca tggcacggtt acccatgcgc ggctgcagtt tgccgttgat
     7981 tttacggatc cagccgcctt tgtatttttc ctggttttcc cagtcagtcg ggaagccctg
     8041 acccggcttg gtttccacgt tgttgaacca cgcgtattcc acgccttcac ggctggtcca
     8101 gacgttttta caggtaactg aacaggtgtg gcagccgatg cacttatcga gattcagcac
     8161 catgccgact tgtgaacgaa ttttcatttt acgctctcct gtacctggtc attgccttcg
     8221 ccatctaacc agtcaatgtt cttcatctta cgcactacaa caaactcatc gcggttagaa
     8281 cctacggtgc cgtagtagtt aaagccgtat gccagatggg catagccgcc gatcatatgc
     8341 gtcggtttcg gcgtgatacg ggtgaccgag ttatggatac caccacgctg ttgggtaatt
     8401 tccgaaccag gcaggttaac gatacgttcc tgcgcgtggt acatcatggt catccctgcc
     8461 ggaacacgct ggctgacaac cgcacgggca gtcagagcac cgttgctgtt gaagacttca
     8521 atccagtcgt tatcggcgat acccagatct ttggcatcgg cttcactcaa ccagaccacc
     8581 ggaccaccgc gacctaaagt cagcatcagc aggttgtcgc tgtaggtgga gtggataccc
     8641 cacttctggt gcggcgtcag gaagttgagc gctttttccg ggttgccgtt ggatttctgg
     8701 cctatcactt ctttcaccga acgggtgtcg atcggcggac gataaaccag caggctttca
     8761 ccgaaatcac gcatccactg gtgatcctga tacagttgct gacgaccaga gagcgtacgc
     8821 catgggatca gctcgtgaac gttggtgtaa ccggcgttgt aagaaacgtg ttcatcttcc
     8881 agaccagacc aggtcgggct ggagataatt ttgcgcggct gtgcctgaat atcgcggaag
     8941 cggatcttct cgtcttcttt atttagcgcc agatgcgtat ggtcacgacc ggtaaattcg
     9001 ctcagggcag cccaggcttt cacggctacc tgaccgttgg tttccggtgc cagtgtcagg
     9061 atcatctctg ccgcatcaat tgcggtgttc agcatcggct ggcctttcgc cggaccttcc
     9121 gctttggtgt agttgagctt acgcagcaga tccatctcgc tctgggtgtt ccaggcaatc
     9181 cctttaccgc cattaccgat tttctccatc agcgggccga tagaggtaaa gcgttcgtaa
     9241 gtcgccggat aatcgcgctc tacgaccata atgtgtggcg cggttttacc tgggatcagg
     9301 tcgcactcgc cttttttcca gtctttcaca tccagcggct gcgccagttc agcggcagag
     9361 tcatgctgga taggcagcgt gacgatgtcg gtttctttac ccagatggcc gacgcacact
     9421 tcggagaatt tcttcgcgat ggctttgtag atttcccagt cgcttttcgc ttcccaggcc
     9481 ggatcgaccg ccgcagacag cgggtgaata aacggatgca tatccgaagt attcatgtcg
     9541 tctttttcgt accaagtcgc cgttggcaaa atgatgtcgg aatagagaca ggtgctcgac
     9601 agacggaagt ccagcgtaac caccagatcc agcttgcctt ccagaccatt gtcctgccag
     9661 tccacttctt ccggcttcac gccgccctgt tgccccagat ctttaccctg gataccgtgc
     9721 tccgtcccca gcaggtactt gagcataaac tcatgacctt taccggaaga accgagcagg
     9781 ttagaacgcc agatgaacag gttgcgcggg tggtttttac cgttttctgg ttgttctgcc
     9841 gcaaaacgga tggaaccctc tttcagggat ttcaccgtat agtccaccgg attcatcccg
     9901 gctttttccg cttcgccagc gatagtcagc gggttagtgc ctaactgcgg tgcagacggc
     9961 agccagccca tgcgttccgc acggacgtta aagtcgatca agtgtccggt atagcgggat
    10021 ttgtccgcca tcggtgacag caactcttcc gccgtgacgg tttcataacg ccactggctg
    10081 gagtggttat agaaataaga agtgctgttc atgtgacgcg ccggacgctg ccagtcaagg
    10141 gcaaacgcca gcggctgcca gccggtttgc ggacgcagtt tttcctgacc tacatagtgc
    10201 gcccagccgc ccccgctctg accgacacag ccgcagaaaa tcagcatgtt gatcagacca
    10261 cgatagttca tatcgaggtg ataccagtgg ttcagccccg caccgacgat aatcatcgaa
    10321 cgaccgtgcg ttttatcagc gttatcggca aattcacggg cgatgcgaat aatctggctg
    10381 cgagaaacgc cggtaatctg ctcggcccag gccggggtat aagctttcac atcgtcatag
    10441 ctggttgcac agttaacgtc gttcaggcca cgttccagac cgtagtttgc cagcgtcaga
    10501 tcataaacgg tggtcaccag ggcggtgctg ccatcagcca gttgcaggcg tttcaccggc
    10561 agtttgtgca gcagcacgtt ttccagttcc actttgttga agtgttccgt gccgtcgcca
    10621 ccaaagtacg ggaagcctac ctcagcgatc tcatcctggc tacccagcag gctcagttgc
    10681 agctcggttt cttcgccagt tttgccgtcg cgctgctcaa gattccattt gcccttctcg
    10741 ccccagcgga agccaataga accgttcggc gcaaccattt cgccattggt attaaaggcg
    10801 acagttttcc atttcggatt gttttcctgg cccagcgcat caaccagatc agcagcgcgc
    10861 aacatacgac ctgcagcgta gtaaccgtcg cgttcttcca gcatcaccag catcggcatg
    10921 tcggtgtagc gacgcacata gtcggtgaaa tactggcttg ggttgtcgag gtggaattca
    10981 cgcagcatta cgtggcccat cgccagcgcc attgccgcat cggtgccctg tttcggtgcc
    11041 agccacagat cgcacagttt ggcgatttca gcgtagtctg gtgtgacggc aacagttttg
    11101 gtgcctttgt aacgcacttc agtaaagaag tgagcatccg gggtacgcgt ctgcggcacg
    11161 tttgaacccc aggcgatgat gtagctggag ttataccagt cagcagattc cggtacgtca
    11221 gtttgctcgc cccaggtttg cggagacgca ggaggcaggt cgcagtacca gtcgtagaag
    11281 cttaagcaag taccgccaat cagcgagaga tagcgtgcac ccgatgcgta agaaaccatc
    11341 gacattgccg gaattggcga gaagccagcg acacggtccg ggccgtagtt tttgatggtg
    11401 taaacgttag atgcggcgat cagttcgttc acttcctgcc aggaagaacg aacaaaacca
    11461 ccgcgtccac gcgcctgctt aaagcttttc gctttatcgg cgtcttcaat gatagaagcc
    11521 catgcctcaa ccggatcgct atgcagcgcc ttcgcttcac gccacatttt catcaggcgt
    11581 ttgcgcatca tcgggtattt caggcggttg gcgctgtaaa gataccagga gtagctggca
    11641 ccgcgcgggc agccgcgagg ttcatggttt ggcagatccg gacgggtacg cggatagtca
    11701 gtctgctggg tttcccaggt gaccagaccg tttttgacgt agattttcca gctgcaggag
    11761 ccggtgcagt ttaccccgtg ggtagagcgg acgattttgt catgctgcca acgctggcga
    11821 tatccatcct cccagtcacg gttggtattg agaagctggc catgcccatc ggcaaaggtt
    11881 tcacccttct gtttgaagta gcgaaaccgg tccaggaatt tactcatcgg ttttctcctg
    11941 tgggagcctg tcggccctct gataaatcga cattgctaat gtttaagggc aaggtaacgc
    12001 tctgaaacag cgtgggaatt gataacgatc aagagtgatg gggatgaaaa ataaagtaaa
    12061 ttcctaagat acccctttag gataattaac agacaccaca taacagact
//
LOCUS       DAJSKJ010000121        11855 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000121 DAJSKJ010000000
VERSION     DAJSKJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.121"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            6..602
                     /locus_tag="ON345_002801"
     CDS             6..602
                     /locus_tag="ON345_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR3072836.1"
                     /translation="MNYPAEYEIGDIVFTCIGAALFGQISAESNCWSNHVGIIIGHNG
                     EDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKL
                     YHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGS
                     IPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(604..1125)
                     /gene="ruvC"
                     /locus_tag="ON345_002802"
     CDS             complement(604..1125)
                     /gene="ruvC"
                     /locus_tag="ON345_002802"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR3072837.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1160..1900)
                     /locus_tag="ON345_002803"
     CDS             complement(1160..1900)
                     /locus_tag="ON345_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3072838.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1929..2381)
                     /gene="nudB"
                     /locus_tag="ON345_002804"
     CDS             complement(1929..2381)
                     /gene="nudB"
                     /locus_tag="ON345_002804"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR3072839.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2499..4271)
                     /gene="aspS"
                     /locus_tag="ON345_002805"
     CDS             complement(2499..4271)
                     /gene="aspS"
                     /locus_tag="ON345_002805"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR3072840.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4581..5147
                     /locus_tag="ON345_002806"
     CDS             4581..5147
                     /locus_tag="ON345_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3072841.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQLVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5144..5962
                     /locus_tag="ON345_002807"
     CDS             5144..5962
                     /locus_tag="ON345_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR3072842.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKL
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWVQL
                     PATFGPRDLPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHTVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHILWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6015..6410
                     /locus_tag="ON345_002808"
     CDS             6015..6410
                     /locus_tag="ON345_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR3072843.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6451..7194
                     /gene="cmoA"
                     /locus_tag="ON345_002809"
     CDS             6451..7194
                     /gene="cmoA"
                     /locus_tag="ON345_002809"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR3072844.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRNIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HQAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7191..8162
                     /gene="cmoB"
                     /locus_tag="ON345_002810"
     CDS             7191..8162
                     /gene="cmoB"
                     /locus_tag="ON345_002810"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR3072845.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8197..10626)
                     /gene="torZ"
                     /locus_tag="ON345_002811"
     CDS             complement(8197..10626)
                     /gene="torZ"
                     /locus_tag="ON345_002811"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR3072846.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGCANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10651..11751)
                     /locus_tag="ON345_002812"
     CDS             complement(10651..11751)
                     /locus_tag="ON345_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR3072847.1"
                     /translation="MRGKKRIGLFFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMNDNAAHELESQAATSVTNDAHIYSFKPSRIGELVTVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2696 a         3150 c         2895 g         3114 t
ORIGIN      
        1 tgaatattaa ttaccctgct gaatatgaaa ttggtgatat cgtctttaca tgtataggtg
       61 ctgccttatt tggtcaaata tcagctgaat caaattgctg gagtaatcac gtcgggatca
      121 ttatcggtca taacggtgaa gacttcctgg ttgcagaaag ccgggttccc ctttcaacta
      181 tcaccacgct gtctcgtttt attaaacgtt cttctaatca acgctatgct ataaagcgat
      241 tagacgccgg gcttacagaa caacaaaaac aacgaattgt tgaacaggtt ccctcccggc
      301 tacgcaaact ttaccacacc ggttttaaat acgaatcttc gcgccagttc tgttcaaaat
      361 tcgtttttga tatttataaa gaggcgctat gtattccggt gggtgaaata gagacgtttg
      421 gagaactgtt aaatagcaat ccgaatgcaa aactcacttt ctggaaattc tggttcttag
      481 gttctattcc gtgggagcgt aaaaccgtca cgccagccag tttgtggcat catccgggtt
      541 tggttttgat tcatgcggag ggagtagaaa cgcctcagcc ggaactgacc gaggcggtat
      601 aacttaacgc agtcgccctc tcgccaggtt cagccgcgat tcgctcatct gcatcgcatt
      661 ctggctgacg tgacaatggg taatcgcgat agctaacgca tcggcagcat ccgcctgtgg
      721 attagcgggc agtttcagca aggtgcggac catatgctgc acctggcttt tttcggcact
      781 accaatacct accaccgttt gctttacctg acgtgccgca tattcaaata ccggcaattc
      841 ctgattcacc gccgccacaa tcgccacgcc gcgcgcctga cccagtttca gggctgagtc
      901 agcgttcttc gccataaaga cttgttcaat ggcgaaataa tcaggctgga actgggtgat
      961 gatttccgtc acgcccgcat agatgagctt cagacgagac ggtaaatcat ccactttggt
     1021 gcgtatgcat ccgctaccca ggtaggacaa ttgcctacct acctggcgga tgacgccata
     1081 gccggtcacg cgcgaacccg ggtcaatgcc gagaataata gccatcacgc gtctccgttt
     1141 tgctgtttag caggccccat cagagagtcg ctgcgacctc atcagagatt tcaccgttat
     1201 ggtagacttc ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc atcagtttcg
     1261 gtgcggtttc tgcatccata tcagctttgg tggacgggat catggaaact tccgcgctgt
     1321 ctgctttcag acctgccgct tccagagcgt cgcgcacttt acccatttct tcccatgcag
     1381 tgtagacgtc aatcgcgccg tcatcgtagg tcacaacatc ttcagcacct gcttccagtg
     1441 ctgcttccat gatggtgtct tcatcgcctt tctcgaagga gatcacgcct tttttgctga
     1501 acagataagc tacggaacca tcagtaccga ggttaccgcc acatttactg aatgcatgac
     1561 gcacttcagc aacggtacgg ttgcggttgt cagacagaca ttcaatcatg attgccgtgc
     1621 cgccaggacc gtaaccttcg tagatgatgg tttccatgtt tgcatcatca tcaccgccca
     1681 caccacgtgc aattgcgcgg ttcagagtgt cacgggtcat gttgttagac agtgctttat
     1741 caattgctgc acgcagacgc gggttagcgt ccggatcgcc accgccgagc ttagctgcag
     1801 ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg cttagcatcc tgcgcagctt
     1861 tacgatgtct ggtgttggcc catttactat gacctgccat aaaaatatct ccagatagcc
     1921 ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg cctgccggtt gctccaggac
     1981 ttagtgagcg ccgccgcagc aggcgcatca agccacttgt aggccagatg ttcagtgaag
     2041 acgacctggc gttcgtgcgg gagcgcaaga cagaaccatg attccgtatt acgcgtcacg
     2101 cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa attctaccgt gcgctgacag
     2161 tcaattaagg tcagttgttc agcgacaaca tcgatggtga cctcttcctt tacttcgcgc
     2221 atggcagctt gcggcgcggt ttcaccctct tccacgctgc cggttaccga ctgccagaaa
     2281 tcgggatcgt cacgccgctg caacatcagc acccgtttcg tatcttgtgc atagatgacc
     2341 actaagatcg aaacgggacg cttatacact ttatccttca cgctgcctct tcgttgactg
     2401 ccttcgctca tcccattcac atagttatct atgctcatgg gagttcactc agttgccgcc
     2461 tcgatgcaac gcgaatgatt tcgtgtattt gagtcatatc agttattctc tgccttcttc
     2521 acaacctgaa tgctcagctc agccagtgca gtcgggttag caaagctcgg tgcttcagtc
     2581 atcagacatg ctgccgccgt ggttttcggg aaggcgataa cgtcacggat gttgtcggtg
     2641 ccggtcagca gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg cggcggagta
     2701 ccgtatttca gggcgtcgag caggaagccg aatttctcgc gctgttcctc ttcgttgata
     2761 cccagaatac caaacaccgt ctgctgcata tcaccattat ggatacgcac agaaccaccg
     2821 cccacttcgt aaccattgat gaccatatcg taagcgttcg ccaccgcatt ttctggtgca
     2881 gctttcagtt ctgcagcagt catgtctttc ggtgaggtga acggatggtg cattgccgtc
     2941 aggccgcctt caccgtcgtc ttcaaacatc gggaaatcga taacccacag cggtgcccat
     3001 ttgctttcgt cggtcagacc aaggtcttta cccactttca ggcgcagtgc gcccatcgcg
     3061 tcggcaacaa ttttcttatt gtcggcaccg aagaaaatca tatcgccatc ttgcgcggca
     3121 gtacgatcca ggatggcttc gatgatttcg gcattaagga acttagctac cgggctgttg
     3181 ataccttcca gacctttcgc gcgttcgtta actttgatgt aagccagacc tttcgcgccg
     3241 tagattttaa cgaagttacc gtattcgtcg atctgcttac gggtcagcga tgcgccgccc
     3301 ggaacgcgca gagccgctac gcgacctttc ggatcgttcg ccggacctgc aaatactgca
     3361 aactcaacag atttcagcag atcggcaacg tcggtcagtt ccatcgggtt acgcagatcc
     3421 ggtttatcag aaccataacg gcgttctgct tctgcaaagg tcattaccgg gaaatcgccc
     3481 agatccacgc ccttcacttc cagccacaga tgacgcacca gcgcttccat cacttcacgc
     3541 acttgcggcg cggtcatgaa agaagtttcc acatcgatct gagtaaattc aggctgacga
     3601 tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct gatagtaacg gtcaaaaccg
     3661 gacatcatca gcagctgttt gaacaactgt ggggattgcg gcagtgcgta gaatttacct
     3721 ttgtgcacac gagaaggcac caggtagtca cgcgcgcctt ccggcgtggc tttggtcagc
     3781 atcggagttt cgatgtcgag gaaaccgtgg tcatccataa aacggcgcac caggctggtg
     3841 attttagcgc gggttttcag gcgctgagcc atttccgggc gacgcaggtc gaggtagcgg
     3901 tatttcagac gcgcttcttc ggtgttgacg tggttagagt caagcggcag aacatctgcg
     3961 cggttgatga tagtcagcga ggacgccagc acttcgattt cgccggtcgc catatcgcgg
     4021 ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtga cctgaatgca gaactcatta
     4081 cgcagttcag aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa tacctgcacg
     4141 ataccttcgc ggtcgcgcat atcgatgaag atcaggctac caagatcacg acgacggttg
     4201 acccaaccac acagagtcac ctgctgcccc acgtgggaca aacggagctg tccacaatat
     4261 tctgtacgca tgagatatcc cttaacttag ctgccggcgg atgccccctg ctgcgcaggt
     4321 gaccaagtcg cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg ctattatact
     4381 ggaaattctg ccgcaccgta agagcctggc ccgcgctgga acgcctcgtt accactttat
     4441 atcgggcctg aaatcagact ctacgccagt ttgctataaa ggtgttgccc gaactcataa
     4501 aaattaacaa aatttgtcgt tccgccatcg gctaatcgca ttaaggtgag aggcacgatt
     4561 ttgttttgtc aggagtcatc atgcttgaac ttaatgctaa aaccaccgcg ctggtggtga
     4621 ttgatttaca agaaggcatc ttaccttttg ccggtggtcc acatactgcc gatgaggtgg
     4681 ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgcgag cggtcagccc gtgtttctgg
     4741 tgcgcgttgg ctggtctgcc gattatgccg aagcattaaa acagctggtt gatgccccct
     4801 cccccgctaa agtgttaccc gaaaactggt ggcaacatcc tgctgcatta ggtgcaaccg
     4861 acagcgatat cgaaatcatc aaacgtcaat ggggtgcgtt ttacggtacg gatctggagt
     4921 tgcaattacg ccgccggggt atcgatacca ttgttttatg tgggatctcg accaatatcg
     4981 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
     5041 atgcctgtag tgccgccagc gccgagcagc acaataacag tatcaaccat atctacccac
     5101 gcatcgcccg tgtgcgcagt gtggaagaga tcctcaacgc attatgattt acatcggtct
     5161 gccgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
     5221 ccgccacttt aactgtgtgg agggcaacac cacgctttac gccctgccca aactcgaggt
     5281 tgtcctgcgc tggcgtgagc agaccacaga tgacttccgc ttctgtttta agtttccggc
     5341 gaccatttcg catcaggcag cattacggca ttgcgatgat ttagtgactg aatttttgac
     5401 ccgcatgtca ccgttagcgc cgcgcattgg gcaatactgg gtgcaactgc ctgccacatt
     5461 tggcccacgg gatctgcctg cgctttggca ttttctcgac tctcttcccg gcgagtttaa
     5521 ttatggtgtg gaagtccgcc atccacagtt tttcgccaaa ggggaagagg aacaaacact
     5581 taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc gcccggttca
     5641 tgcagcacgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc
     5701 ggtacatact gtactgacgg cgacaaatcc gctgatccgt tttatcggta gtgatgatat
     5761 gacgcaaaac cgggaattat ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac
     5821 cactacgcct tatctttttt tacatacgcc agatattgcc caggccccgg aactggtaca
     5881 tatcctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc
     5941 acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt
     6001 taaagggagt ttgtatggta agcgcgctgt atgccgtttt aagtgcgttg ttattaatga
     6061 agttctcttt tgatgtcgtt cgcctgcgaa tgcagtaccg cgttgcctat ggcgacggcg
     6121 gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccca
     6181 tcgcgattgt gttgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata
     6241 tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc
     6301 tgttccgctg gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc
     6361 tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac
     6421 gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc
     6481 cctatcgcca gactgggcga ctggaccttt gatgaacggg tagctgaagt cttcccggat
     6541 atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg tatgttagcc
     6601 gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg
     6661 acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgacaac
     6721 tctccggcga tgattgaacg ctgccgtcgt catattgacg cctataaagc ccctacgcca
     6781 gtagacgtta ttgaaggtga tattcgcaat atcgccattg aaaacgcatc gatggtggtg
     6841 ctgaatttta ccctgcaatt cctggaacct tccgagcgtc aggcgttact ggataaaatt
     6901 tatcaagggc tgaacccggg cggtgcgctg gtgctttcag aaaaattcag tttcgaagat
     6961 gccaaagttg gtgaattgct gttcaacatg caccacgact ttaaacgtgc taacggttac
     7021 agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct aaccgattct
     7081 gtggaaaccc ataaagcacg cctgcatcaa gctggttttg agcatagcga gctgtggttc
     7141 cagtgcttta actttggttc cctggtggca ttaaaagcag aggacgctgc atgatcgact
     7201 ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctg gaaacgctgc
     7261 ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag cagtggtcca
     7321 acgcggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg catagcgtaa
     7381 ccgccgaaag cgaagagcca ctgagcgccg ggcaaattaa acgcattgaa acgctgatgc
     7441 gcaacctgat gccgtggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg
     7501 aatggcgttc cgactggaaa tgggatcgcg ttctgcccca tctttctgat ttaaccgggc
     7561 gcaccattct tgatgtcggc tgtggcagcg gttatcacat gtggcgcatg attggcgcag
     7621 gcgcacatct ggcggtgggg atcgacccta cgcaactgtt tctgtgccag tttgaagcag
     7681 tgcgtaaatt gttaggtaac gatcaacgtg cgcatctgct gccgttaggc attgaacaac
     7741 ttccggcact gaaagccttt gataccgtct tttcgatggg cgtgctttat catcgtcgtt
     7801 caccgctgga gcatctctgg cagttgaaag atcaactggt gaatgagggt gaactggtgc
     7861 ttgaaacgct ggttattgat ggcgatgaga acacggtgct ggtgccgggc gatcgttacg
     7921 cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga
     7981 agtgtggttt tgtagatatc cgcattgcgg atgtgagcgt taccactaca gaagagcagc
     8041 gacgcactga atggatggtt accgagtctc tgtccgattt cctcgaccca catgatccga
     8101 gtaagacagt tgaaggttat ccggcaccta aacgcgcggt gctgattgcg cgcaagccgt
     8161 aaagattagc gcccgtttcc gggcgctttt cacgtcttat gcctgaacag ctggctgatc
     8221 aaacgccgtt aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt
     8281 accggcacat gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcaca
     8341 gccgttttta cacaagccat tttccagatc tggccatgca ccttcatgca cgcataccac
     8401 gccttttttg atcccgtcag tcaccaccgc gcctgtcaga atctgaccac gtttgttcca
     8461 gacacgcacc agatcgccat tcgcaatacc aaagcgagca gcatcttcgg tgtgaatagt
     8521 gatcggttca cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact
     8581 gtgtaaacgg tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc
     8641 tttccactca tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt
     8701 ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac
     8761 cggatcggcg cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat
     8821 ttcgatcagt ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc
     8881 acgggcacct ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc
     8941 ttcggtatag atctcttttc cgccaggttt cagtaattcc gcaagttcgg caaacacgtc
     9001 aaaatcgttt cgcgcttcaa attgcggagc gacagcctgc ttcatcggta caatatgctg
     9061 gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa
     9121 tacgatatct gcatgtttag cagctgcggt ccagtagcat tcagaaacga cgatcatctc
     9181 cggtttctgc catgctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc
     9241 acccgcccac cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata
     9301 tttgccgcca ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc
     9361 gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac
     9421 gccgccaaca cgcgtcgggt tgccaccgtt ggagtaatga taagagaaac caaaaccgcc
     9481 gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg
     9541 ttcaccatat tgctggcgct gaattcccca gcctgccatc agcattgtgc gattcgccgc
     9601 catcagttca gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca
     9661 ggccgcgctc ttcggagtat tatcgctctt acccgtcaga tactcttcaa actgcggata
     9721 cccggtggtg tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg
     9781 cgcaatccct aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt
     9841 atcgccaaag aactcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc
     9901 agattttttc agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat
     9961 ttttaaggta ttcagcgggt tcatccccca cagcaccaca acctggctgt tttccagaat
    10021 cagcggccag gaggtctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat
    10081 cacctgtgct gccccggttg aataatcgcc gctatgcccg gaataaccgc ctgccaggtt
    10141 catataacgt tgcagtaacg tttgcgcttt atgcaacacg ccagaagagc gccagccgta
    10201 agatccggca aaaatagccg atggtccgtt agctttgcga atgcgatcgt gttgctcatg
    10261 aatcagtttt aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt
    10321 cgccggttgc agtggattat cgagatagct ttttctcacc atcggatgct gaatacgcgc
    10381 cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggaa ttcggtatgg ttttcgccag
    10441 cgcgcctgtt gaagaaacaa tcttgccgtc cttcacttct acgttcatcg ctccccaacg
    10501 tcccgcggtg aggattttac cgcccttctc ttctgcccat gcggggagcg gtgctgccga
    10561 tgtcaccacc agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa
    10621 tgtcataact tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt
    10681 gtactggaag taccgcgtca aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc
    10741 cattcctttg gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caataggcgc
    10801 atggcaacca gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg
    10861 gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc
    10921 gtattcgtca gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa
    10981 tgtggctagc gccaaccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat
    11041 ttgataaccc tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc
    11101 ggttccagga ttcacggtaa ccagctcgcc tatgcgagaa ggtttgaaag aataaatatg
    11161 tgcgtcatta gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatt
    11221 catttttatt tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc
    11281 gtccttttgt gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat
    11341 agcatcaaaa gaatggcaac tacggcaggt tgcagagtca gttgctttta attctttcca
    11401 tactgtttcg gccatttcct ggcgatgagc ttcgaactta tcgtcactgt ctattttgcc
    11461 gctaacaaat tcatgataaa tatctttaga tgcttttaat ttagcaaata aataatccat
    11521 ccctgacttt ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa
    11581 gtggacagtt ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa
    11641 tgctgtatcc gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac
    11701 cacaaccgct atcagcagaa aaaataaccc aatgcgtttt ttccctcgca tagattcctc
    11761 taaatatatt ccagaacacc gaaatcattg tataattaaa tacacaacga attaaccgct
    11821 tgcataactc ggcacaacac tgcctgaaat aatcg
//
LOCUS       DAJSKJ010000122        11528 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000122 DAJSKJ010000000
VERSION     DAJSKJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.122"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(5..1378)
                     /gene="uidB"
                     /locus_tag="ON345_002813"
     CDS             complement(5..1378)
                     /gene="uidB"
                     /locus_tag="ON345_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR3072848.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(1375..3186)
                     /gene="uidA"
                     /locus_tag="ON345_002814"
     CDS             complement(1375..3186)
                     /gene="uidA"
                     /locus_tag="ON345_002814"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR3072849.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGVQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     VHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3575..4165)
                     /gene="uidR"
                     /locus_tag="ON345_002815"
     CDS             complement(3575..4165)
                     /gene="uidR"
                     /locus_tag="ON345_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR3072850.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4394..5161)
                     /gene="hdhA"
                     /locus_tag="ON345_002816"
     CDS             complement(4394..5161)
                     /gene="hdhA"
                     /locus_tag="ON345_002816"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR3072851.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5273..6301)
                     /gene="malI"
                     /locus_tag="ON345_002817"
     CDS             complement(5273..6301)
                     /gene="malI"
                     /locus_tag="ON345_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR3072852.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6476..8068
                     /gene="malX"
                     /locus_tag="ON345_002818"
     CDS             6476..8068
                     /gene="malX"
                     /locus_tag="ON345_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR3072853.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8078..9250
                     /gene="malY"
                     /locus_tag="ON345_002819"
     CDS             8078..9250
                     /gene="malY"
                     /locus_tag="ON345_002819"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR3072854.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9354..10355
                     /gene="add"
                     /locus_tag="ON345_002820"
     CDS             9354..10355
                     /gene="add"
                     /locus_tag="ON345_002820"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3072855.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIDDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10390..11430)
                     /locus_tag="ON345_002821"
     CDS             complement(10390..11430)
                     /locus_tag="ON345_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3072856.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCIA"
BASE COUNT         2782 a         3047 c         2833 g         2866 t
ORIGIN      
        1 aatattaatt agtgatatcg ctgattaatt gctgctgcac ttttttacga ttatcaattt
       61 caaccacgat ttctttgaat tttttatccg tgagcggata aaaccagata ataacgaatg
      121 ccagtagcat aaatccgcaa ggtactaagg caattgatgt gcggatgccc ataataactt
      181 ccggcgtttg cacctgattg gcgatatatc cgcttaatcc caaaataaag gcaggaattg
      241 aacctccgat tgcctgaccg catttacggg taaatgagaa tagtgaatag gtgagccctt
      301 caattcgcac gccggtcagg tattcaccgt attctacggt atcagcttcc agtgcccaca
      361 tcacagtcat agtaacgccc tgaccaattg aagcgatggc caacgcaacc aacgccaccg
      421 gcagtgacca gacggaaacc cagaagaata gcagataacc gcaggttccc agcaaagcgc
      481 caatcaggaa ggtattcttt ttaccgatcc tcgcgaccat ccccggcacc agcggtgccg
      541 atgccacagt accaaccagg ttttgcacca gtaccagcac agtgaacagc ccggtatcat
      601 ttaacacata gcgcacgtag aacaacgacg aggcgctgac cgcgaaggtc gaaatcagca
      661 cacacagcgc accgatgcac aacataaaca gcgggcgatt ccgtttcagg gtttgcagac
      721 tgatcttcaa tgacggctgc gccacgatac gtaccacatt ctcacgcgtc gatttgaagc
      781 agatgaagta aagcaccatt ccggcaatcg ccagcacaat cgtccagaaa tggtataccg
      841 acaccatctc ttccgggctg gagttcttaa tgctcggtcc tatcagaaat gccaggcaga
      901 caaaggttaa tgaagcggca atcccgcgag ccgcgcccag acgggcgcgg gattgtggtt
      961 gttgggtcat cgcggtagca agtgaaccat aaggaatatt caccaggctg tagcaaagcc
     1021 cgaggcccat gtaggtcaaa tatgcataca ccactttgct accatggctc cagtcggtcg
     1081 gcacccagaa taccagcacg ctgaagatca ttaacggcgc agtaccgaag agtaaaaacg
     1141 ggcggaattt tccccagcgg gtattcacac tgtccaccac tcgtccggca aagacgtcgg
     1201 cgaaggcatc gaatacccgc accagtaaca gcatggtgcc cgccgcagcg gcaccgacgc
     1261 cagcgacgtc ggtgtagtaa ctcaatagga agagcgcccc cattgcgaag gcgaagttat
     1321 tggcgacgtc accgaggctg tagccgacga tggtgcgcca ggagagttgt tgattcattg
     1381 tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag
     1441 aaaagccgcc gacttcggtt tgcggtcgcg agtgaagatc cctttcttgt taccgccaac
     1501 acgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgtt caccgacgac
     1561 ggcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact gatactcttc
     1621 actccacatg tcggtgtaca ttgagtgcag cccggctaac gtatccacgc cgtattcggt
     1681 gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt ccagtacctt
     1741 ctctgccgtt tccaaatcgc cgctttggac ataccatccg taataacggt tcaggcacag
     1801 cacatcaaag agatcgctga tggtatcggt gtgaacgtcg cagaacatta cattgacgca
     1861 ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg cgaaatattc
     1921 ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca ccacgcttgg
     1981 gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt aagtgcgctt gctgagtttc
     2041 cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat
     2101 gcctaaagag aggttaaagc cgacagcagc agtttcatca atcaccacga tgccatgttc
     2161 atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt aggagttggc
     2221 cccaatccag tccattaatg cgtggtcgtg caccatcagc acgttatcga atcctttgcc
     2281 acgcaagtcc gcatcttcat gacgaccaaa gccagtaaag tagaacggtt tgtggttaat
     2341 caggaactgt acgcccttca ctgccactga ccggatgccg acgcgaagcg ggtagatatc
     2401 acactctgtc tggcttttgg ctgtgacgca cagttcatag agataacctt cacccggttg
     2461 ccagaggtgc ggattcacca cttgcaaagt cccgctagtg ccttgtccag ttgcaaccac
     2521 ctgttgatcc gcatcacgca gttcaacgct gacatcacca ttggccacca cctgccagtc
     2581 aacagacgcg tggttacagt cttgcgcgac atgcgtcacc acggtgatat cgtccaccca
     2641 ggtgttcggc gtggtgtaga gcattacgct gcgatggatc ccggcatagt taaagaaatc
     2701 atggaagtaa gactgctttt tcttgccgtt ttcgtcggta atcaccattc ccggcgggat
     2761 agtctgccag ttcagttcgt tgttcacaca aacggtgata cgtacacttt tcccggcaat
     2821 aacatacggc gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct ccatcacttc
     2881 ctgattattg acccacactt tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg
     2941 ctggcctgcc caacctttcg gtataaagac ttcgcgctga taccagacgt tgcccgcata
     3001 attacgaata tctgcatcgg cgaactgatc gttaaaactg cctggcacag caattgcccg
     3061 gctttcttgt aacgcgcttt cccaccaacg ctgatcaatt ccacagtttt cgcgatccag
     3121 actgaatgcc cacaggccgt cgagtttttt gatttcacgg gttggggttt ctacaggacg
     3181 taacataagg gactcctcat taagataaaa atactggtca acctttaatc tgattagtta
     3241 accaattctg tgttgcacac agttatctgt gagaaaccca tggaagagaa atggaaataa
     3301 aaagaactga caccgcgatc aaaaaaccaa aaactgcaat acccacagcg tgatattgat
     3361 cgcattaatc tttaaaacgg cgttgacaga agccataaaa taagcaaccg aacgttcgtt
     3421 cgagcatatt gggaacgata aactctcatg acatatggct acggtgaata tttttggggg
     3481 ggaattctcc gatcccggct agattgtatt ttgtacaggg aaattaaagt atgaaacgcg
     3541 ggcacagtta tcaccagccc gcgaaatgag aggatcagga tgcggttaag ataccgccaa
     3601 tcatcgcgcg taatccctga gcaaacgacg cttcacgcgc cagatcttcc gcttcgatat
     3661 cggccagtac accgtaggtc agatccagca gtaaacgtga agtcattgac gtgttgatgt
     3721 ctgggcttat ctcgcctttt tgctgggcat cacgcatccg ctgggcaaca aattcagtga
     3781 tcgtcatatg cttattttta agcatggcgg cgacctgtgg gttacgcatc ccttccgcca
     3841 taatttcgac caccagcgcc cgttgcccaa aggcttcatg ggtgagagag acaatagact
     3901 cgaccatata gtcaacaaaa tgaatccctt caatcggttc ccggaaacgg gccagcgccc
     3961 tctcctggtc ctgtaagata atcgcctgaa tcaaggcttc tttggagatg aaatggtgat
     4021 agagcgtccc gggactaatg gcgcaggatt tacagatggc tttcatcgag gcactgtgaa
     4081 atccattttc tgaaaaaatc tctctggcag cattgaggat ccgggtccgt gtcggttgtg
     4141 cttcagtctg catgttatcc atcattaaac cattatagtt agcgtgaata actagtttac
     4201 cgcgttgtca ggtgagcgcc tatcattgct gttgaatagt gcgaaggcac actctattcg
     4261 tatgcttttc atccagctat tggttaacca atagccgtga tttggctgcc agttgtttag
     4321 cagagacgtt gatgaattgc acacgctatc ccggtaaaca acacatacgg ctgtttaccg
     4381 gttcgttagt gtattaattg agctcctgta ccccaccacc ggagacggtg agaatttgtc
     4441 cgcttaccca gctggctgca ggcgagcaaa ggaacagcgc cgcgttagca atatcttgcg
     4501 gttggcccag acgtctgatc ggcgtgtgct gtaacatttt ttgttcaatt tctggtgtaa
     4561 taacggattt cagggcatcg gttaatattg cccccggcgc aatgccattc acccgaatat
     4621 ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca gctttagatg
     4681 atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg atggtcagaa
     4741 taacgccacc gccatttttt tccatttctg gcgcaacaag ttgtgacaga tggaaaaaag
     4801 aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca aacggtttag
     4861 gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagctta ctgacggcaa
     4921 agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacggcag gcaaatgcct
     4981 gaccacccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg ttaatatcac
     5041 tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta ccaatacctg
     5101 cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca gaattaaaca
     5161 cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc cacatttttt
     5221 atcattaacc ttgaggaaag gacactacca ggtatagcag ataaagagag gattatttcg
     5281 ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca tgggtgattt
     5341 tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg ctggcccagg
     5401 taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc agcgaaacct
     5461 gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct ttgagcaaac
     5521 caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg atggtcgggt
     5581 tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa gtgcactcca
     5641 acacccaatc gctatgaaac ggcaggccaa attttagtag agttgcacaa tagcccccca
     5701 cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc cgctgatgcc
     5761 cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc gggcgaaccg
     5821 tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata gctttttctt
     5881 ctgccatccg tcgcaggtca tcgctacttc ctgcagctcc ggcaattacc acaccatcga
     5941 caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcaccgtct ttaccgccgt
     6001 gaagcaaaaa aaccatccgt ccctgcgctt ccagagcttc cgtcaatccg gccgtcaatt
     6061 cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg ctttgcccgc
     6121 cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg gcggcgttaa
     6181 cgcgttctcc tgtggcggta gagattcgcc ctttgccact aagcaccagc gaaacggtac
     6241 ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg gcggtagcca
     6301 tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt tatttatctt
     6361 atacccgcta ttatcgttgc gtaatgtgat ttatgcctca ctaaaatttg ataaaacgtt
     6421 ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc gttttatgac
     6481 ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag gcaaaacctt
     6541 catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg gtagttctct
     6601 tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt tgcaggctat
     6661 ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg tcatgttctg
     6721 tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat tcgctggctt
     6781 cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata aaggcattct
     6841 gccaaccacg gatgccgcgg ttctgaaagc caataacatc cagagcattc ttgggatcca
     6901 gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct ggatgctgca
     6961 tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg gtacgcgctt
     7021 cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc cattagtctg
     7081 gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg cgggtgattt
     7141 cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc tgcatcacat
     7201 tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaagtct gcggtcaaac
     7261 cgtcagcggc gcattgacca tcttccaggc gcaattgagt tgcccgacca ctcacggttt
     7321 ttctgaaagc gccacgcgtt tcctttcgca aggcaaaatg cctgcgtttc tcggcggtct
     7381 gccaggtgca gcgttagcga tgtatcactg cgcgcgcccg gaaaatcgcc ataaaattaa
     7441 agggctgctg atttctggcc tgatcgcctg tgtcgttggc ggcacgaccg aaccgctgga
     7501 attcctgttc ctgttcgtgg cgccagttct gtatgtcatc cacgcgctgt taaccggcct
     7561 cggcttcact gtcatgtctg tgctcggcgt caccatcggt aataccgacg gcaatatcat
     7621 cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca tggtgccagt
     7681 ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta tcacccgttt
     7741 caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa aagccgttgc
     7801 cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat taggcggtgc
     7861 cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg tgaaagatat
     7921 gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag tacaacttaa
     7981 tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag atgaaatggc
     8041 cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt caaaggtcgt
     8101 ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt tcggcactgc
     8161 tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct gcattatcga
     8221 ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga aaaacgatga
     8281 gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa ttgatacgca
     8341 gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc gtcagtggtc
     8401 tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt acaaggccat
     8461 tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg acgactggtt
     8521 ttgcgatatg ggcaagctgg aagccgtgtt ggcgaaacca gaatgtaaaa ttatgctcct
     8581 gtgtagccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg agatcatggc
     8641 tgacctatgc gagcgtcatg gtgtgcgggt tatttccgat gaaatccata tggatatggt
     8701 ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact gggcgttgct
     8761 gacgtcgggc tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg ggattataga
     8821 aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg ggctttcttc
     8881 cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg caccgtggct
     8941 ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata aaatgaacgc
     9001 cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct ggcttgatct
     9061 acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc aagaaaaagt
     9121 cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc gtctcaatgc
     9181 cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg ccatccgcgc
     9241 tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg ctttttaccc
     9301 tgctttgttt ttataatggt gcgcactttt atattcagaa aaagagtgcg accatgattg
     9361 ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt cgtccccaga
     9421 ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc ctggaaacac
     9481 tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt ctgaccaaac
     9541 ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg tcgccgcgtg gcatttgaaa
     9601 acattgatga tgcagcccgt cacggcctgc actatgtcga gctgcgtttt tcaccaggct
     9661 acatggcaat ggcacatcag ctgcctgtag cgggtgttgt cgaagcggtg atcgatggcg
     9721 tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt atgagccgga
     9781 ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac cgtgaccaga
     9841 ttaccgcact tgatttagcc ggtgatgaac ttggtttccc gggaagtctg ttcctttctc
     9901 acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc gaagctgccg
     9961 ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt ggacatggcg
    10021 taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa attggtattg
    10081 aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct gcacatccgc
    10141 tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat cccggcgtac
    10201 agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg ttatcccgcg
    10261 agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc gctgaggaaa
    10321 aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa tggatggtgc aaattgcacc
    10381 atccattttt catgcaatgc acaaagtcgc gcgatgtttg gcggattcga tgcccagctc
    10441 aatcaactcc attacctgga ttgcctggct tgccggaacc ggattttcac catcgccatt
    10501 taacgcatca cgaatagccg catagtaagc cggataattc ccaggtaccg tcaacagcgt
    10561 ttcttcgaca cgttcctcac cttccacgcg ggtaagtacg ccatcacgca tatcgtagcc
    10621 ccagtcttcc tgcggcagac gctcgccatt tttcagacgt tcttcctgtg gatcgaggcc
    10681 atatttcaca taactgcctc gggatccatg cacgatatac cgtgctgact cagcggctgc
    10741 cagcatggta ccgtgtaaaa tgactcgccg ctgtggatag gacaagatgg cgtggaaata
    10801 atcggtcgac tgcgctccgg gccgtaactg tgccaaatct accgtcatgc tgaccggtaa
    10861 accaaatagc gtaatggcct gatcaagaag atgcggtgct aaatcgtacc agataccgct
    10921 gcctgggccg ccctgttcac gccaacgatc gcgcacctgc ggacggaagc ggtcaaaatg
    10981 agactcaaag taagcaactt cacccagcac gccttccgcg agcaaacctt ttagcgtcaa
    11041 gaaatcgcta tcccaacgac ggttatggaa tacagacagc acacgcccca ggctttttgc
    11101 cagcgcatcc agctctcgcg cttgtgacag tgtcacggta aagggtttat caacgaccac
    11161 atgtttaccc gcctcaagcg ccgctttggc taacgggaaa tgggtatcgt tgggtgtagg
    11221 aatgacaatc aggtctatgt tgggatcgtt aaacagatgc ttcggctcag agacaaccgt
    11281 aaccgttggc cagtcggctt ttacttttgt ttcatcactg ctggagatta ccgccagttc
    11341 cagcccgggc gtgcccgcaa tcaggggcgc atggaaggtt ttgctcgcat aaccataccc
    11401 aatcaaccca acacggatgt tgtcgctcat gtcattgcct ctcattgaag tatgatggct
    11461 atttgacact atcctttacc cacgctcaac agtttaataa cctgccagca ataagggatg
    11521 ttgtttaa
//
LOCUS       DAJSKJ010000123        11523 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000123 DAJSKJ010000000
VERSION     DAJSKJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.123"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            228..998
                     /locus_tag="ON345_002822"
     CDS             228..998
                     /locus_tag="ON345_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR3072857.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            989..1462
                     /locus_tag="ON345_002823"
     CDS             989..1462
                     /locus_tag="ON345_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR3072858.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1569..2108
                     /gene="dnaT"
                     /locus_tag="ON345_002824"
     CDS             1569..2108
                     /gene="dnaT"
                     /locus_tag="ON345_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR3072859.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2111..2848
                     /gene="dnaC"
                     /locus_tag="ON345_002825"
     CDS             2111..2848
                     /gene="dnaC"
                     /locus_tag="ON345_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR3072860.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2897..3391
                     /locus_tag="ON345_002826"
     CDS             2897..3391
                     /locus_tag="ON345_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR3072861.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3645..5936
                     /gene="opgB"
                     /locus_tag="ON345_002827"
     CDS             3645..5936
                     /gene="opgB"
                     /locus_tag="ON345_002827"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR3072862.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6076..7098)
                     /gene="lgoD"
                     /locus_tag="ON345_002828"
     CDS             complement(6076..7098)
                     /gene="lgoD"
                     /locus_tag="ON345_002828"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR3072863.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7237..8151
                     /locus_tag="ON345_002829"
     CDS             7237..8151
                     /locus_tag="ON345_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3072864.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8366..9727
                     /locus_tag="ON345_002830"
     CDS             8366..9727
                     /locus_tag="ON345_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3072865.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLNRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGSMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9776..11440)
                     /gene="tsr"
                     /locus_tag="ON345_002831"
     CDS             complement(9776..11440)
                     /gene="tsr"
                     /locus_tag="ON345_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR3072866.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKSSL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMVVADSEENWETF"
BASE COUNT         2785 a         3056 c         2825 g         2857 t
ORIGIN      
        1 ctgattcgta aaggccaaac aataaattca ttatagtgat aatattcatg tgatagtttg
       61 tttgcggcga gagataattc gctttttatc accgagcttg tcccgataac caggattaat
      121 cagcgcaatt ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt
      181 ctcattatct gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc
      241 aacagcgagc cgtaacacgg ctttgtatcc agtgtggatt atttcttttg caacatggtg
      301 cggaaagcgc gttggttgat gagctttcct cacgactggg tcgggcgttg gggatggaca
      361 gcgtcgaaag ttctatctct tcgaacgcca tagtgctgac aaccattaaa gatgggcaat
      421 gcctgacatc gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag
      481 tccagcacat tgtgattctg gcggagcacc atctgctgga ttacaaaggc gtagagaaac
      541 gatttagcca aattcagcca ttacgttacc caagatggct ggtagcctta atggttggcc
      601 tttcttgcgc ctgtttctgc aaactcaata aaggtggctg ggatggtgcc gtcatcacct
      661 tctttgccag tacggccgcg atgtatatcc gccagctgct ggcacaacgt catcttcatc
      721 cacaaatcaa cttttgcctt accgctttcg ccgccaccac catttccgga ttgcttttgc
      781 aactccccac tttcagcaat acccccacca ttgcgatggc cgccagcgtt ctgctgctgg
      841 tgccgggctt tccgttgatt aatgccgtcg ccgatatgtt taaaggccac atcaataccg
      901 gactggcacg ctgggcgatc gccagtctgc tgacactggc tacctgcgtc ggcgtagtga
      961 tggcactgac gatttggggg ctacgcggat gggtgtgatc gaatttctgt tagcgttggc
     1021 gcaggatatg atcctcgccg ccattcctgc ggtcggcttt gcgatggtgt tcaacgttcc
     1081 cgttcgggcg ttacgctggt gtgcgctgct tggcgcgatt ggtcatggtt cacgaatgat
     1141 cttaatgacc agcgggctga atattgagtg gtcaactttt atggcttcta tgctggtcgg
     1201 caccattggt attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc
     1261 ggccgttatc cctatgttcc cgggcatatc ggcttatacc gcaatgattt cggcggtaaa
     1321 aatcagccag ttaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac
     1381 agcttcatcg attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta
     1441 ccgcaagcgc cctcgcgtat aaaattgcct cgcttaagca cacggatgag agacagcctc
     1501 ctctcctccg tgtgttacta taaaagttat ctcccttctc gttcatcgtt ccatatttga
     1561 gaaacagtat gtcttccaga gttttgaccc cggacgtcgt tggtattgac gccctggtac
     1621 acgatcacca aaccgttctg gcaaaagctg aaggcggtgt ggttgccgta tttgctaaca
     1681 atgccccggc gttttatgcc gtcacgcctg cacgcctggc tgaactgctg gcgctggaag
     1741 aaaagctggc gcgtccggga agcgatgtcg ctctggacga tcaactctat caggaaccgc
     1801 aagccgctcc cgttgctgtc cccatgggga aattcgccat gtatccggac tggcaacccg
     1861 atgccgactt tatccgcctg gcggcgctat ggggcgtggc gctaagagag ccggtgacca
     1921 ccgaagaact ggcctcattc attgcctact ggcaggcgga aggtaaagtc tttcaccatg
     1981 tgcagtggca acaaaaactg gcgcgcagcc tgcaaatcgg tcgtgccagc aacggcggac
     2041 tgccgaaacg agatgtgaat acggtcagcg aacctgacag ccaaattcca ccaggattca
     2101 gagggtaacg atgaaaaacg ttggcgacct gatgcaacgc ctgcaaaaaa tgatgcctgc
     2161 ccatatcaaa cctgcattca aaacgggtga agagctactg gcctggcaga aagaacaagg
     2221 ggcgatccgc tccgccgctc tcgaacgtga aaatcgggcg atgaaaatgc agcgcacctt
     2281 taaccgctcc ggtattcgtc cactgcatca gaactgctcc tttgagaact atcgcgttga
     2341 gtgtgaaggg cagatgaatg cgttaagcaa agcgcgccag tatgtcgaag agtttgacgg
     2401 caacatcgcc agcttcatct tttctggtaa gccaggaacc ggcaaaaacc atctggcggc
     2461 ggcaatctgc aacgagctgc tgctacgcgg taaatccgta ttgatcatca ccgtggccga
     2521 tattatgtcg gcgatgaaag ataccttcag gaatagcggt accagtgaag aacaactgct
     2581 taacgatctg agcaacgttg atctgctggt gatcgatgag atcggcgtgc agaccgaatc
     2641 gaaatacgaa aaagtgatca tcaaccagat cgtcgatcgc cgctcttctt ccaaacgccc
     2701 aaccgggatg ctgaccaaca gtaatatgga agagatgacc aagttgctgg gcgaacgcgt
     2761 gatggaccgt atgcgcctgg gtaacagttt gtgggtgatc ttcaactggg atagctaccg
     2821 tagccgggta acaggtaaag agtattaatc cccgtttctg gcggtatact gcgacacaat
     2881 tccaccttga gtaatgatga tgaaaactgt taaacatctt ctgtgctgtg ccattgccgc
     2941 cagcgcatta atttccaccg gggtgcatgc tgcgtcctgg aaagatgcgc tctccagcgc
     3001 cgccagcgaa cttggcaacc aaaacagcac gacacaggaa ggcggttggt cgctcgcgtc
     3061 attaactaac ttgcttagca gcgggaacca ggccttaagc gcagataaca tgaacaacgc
     3121 cgcaggcatt ctgcaatact gcgcgaagca aaagctggcg tcggtaaccg atgccgaaaa
     3181 catcaagaac caggtgctgg aaaagctggg cctgaacagt gaagagcaaa aagaagacac
     3241 caactatctg gatggcattc aggggttgct gaaaaccaaa gatggtcagc aactcaatct
     3301 ggataacatc ggaacgactc cgctggcaga aaaggtgaaa accaaagcct gcgatctggt
     3361 gttaaagcag gggctgaact tcatttcctg aaccgcgaaa cgccgcgttt tagctgttac
     3421 ccggctgacg cggctctctc ctgccctttt ccctgctcct ggacggtttt ccccctgtta
     3481 cacgggaagc gaccgcgatc aaaaaagtct gtttataaac taattcctaa caacatcgca
     3541 tttttttgac actagaattg cagcaatatg gcgacgccat tacattgtgc ttctcaaaaa
     3601 gcttaaacgt tggctaaacg gcccggttta gtgaggatcg cccgttgtca gaactactct
     3661 ctttcgccct ttttctcgcc tctgtgctga tttacgcatg gaaagcggga cgtaacacct
     3721 ggtggtttgc agccacatta acggtgctgg ggctatttgt cgttttaaat atcaccctgt
     3781 ttgccagcga ctattttact ggcgatggta ttaacgacgc ggttctctat accttaacca
     3841 acagcctgac cggtgctggc gtcagcaaat acatcctgcc gggtatcggc attgtgctgg
     3901 ggctgacagc ggtgttcggt gcgctgggct ggatcctgcg ccgtcgtcgc catcatccgc
     3961 accattttgg ttacagcctg ctggcgctct tactggcgct gggttcggtg gacgccagcc
     4021 cggcatttcg tcagataacg gaactggtga aatcccagtc acgcgatggc gacccggact
     4081 ttgcggctta ttataaagag ccgtcgaaaa ctatccctga cccgaaactc aacctggttt
     4141 atatctacgg cgaaagtctc gagcggacct attttgataa cgaagctttc ccggatctca
     4201 cgcctgaact gggcgcgttg aaaaatgaag gcctggattt cagccacacg cagcagctgc
     4261 caggaacgga ttacacgatt gcgggcatgg tggcttctca gtgcggcata ccgctgtttg
     4321 ccccctttga aggcaacgcc tccgcctctg tctccagctt cttcccgcag aacatctgtc
     4381 tgggcgatat cctgaaaaac tcgggttatc agaactattt cgtgcagggc gcgaatctgc
     4441 gttttgccgg taaagatgtg ttcctgaagt cgcatggctt cgaccactta tacggctcag
     4501 aagagctgaa aagcgtggtg gccgacccgc actatcgcaa cgactgggga ttctacgacg
     4561 ataccgttct cgatgaagcg tggaaaaagt ttgaagagct ttcccgctca ggtcagcgat
     4621 tctcactgtt taccctgaca gtcgataccc atcacccgga tggttttatc tcgcgtacct
     4681 gtaaccgcaa aaaatatgat tttgacggta agccgaacca gtcattcagc gcggtaagtt
     4741 gcagccagga gaatatcgcg gcgtttatca acaaaatcaa agcgtcaccg tggtttaaag
     4801 ataccgtcat cgtcgtctct tctgaccatt tagcgatgaa caacacggcg tggaaatacc
     4861 ttaataagca ggatcgcaat aacctgtttt ttgtcattcg tggcgacaag ccgcagcaag
     4921 agacgctggc agtgaagcgt aacacgatgg ataacggcgc gacggtgctg gacattctcg
     4981 gtggcgataa ctatctcgga cttggtcgta gcagtttatc cgggcagtca atgtcggaaa
     5041 tcttcctcaa tatcaaagag aaaacattgg cgtggaagcc ggatatcatc cgcctgtgga
     5101 aattccctaa agagatgaaa gagttcacca tcgaccagca gaaaaacatg attgccttct
     5161 cgggtagcca tttccgtttg ccgctgctgt tgcgggtttc agacaaacgc gtggaaccgc
     5221 tgccggaaag cgaatactca gcaccgctgc gtttccagct ggccgatttc gctccacgcg
     5281 acaatttcgt ctgggttgac cgttgctata agatggcaca actctgggct ccggaactgg
     5341 cactctccac cgactggtgt gtctcgcaag ggcagcttgg cggtcagcaa attgttcagc
     5401 atgttgacaa aacaacatgg cagggcaaaa cggcatttaa agatacggtc atcgacatgg
     5461 cgcgttataa aggcaatgtc gatacgctga aaattgttga taacgatatt cgctacaaag
     5521 ccgacagttt catcttcaac gtcgccggtg cgccagaaga ggtgaaacag tttagtggga
     5581 tttcccgtcc agagtcgtgg ggccgctggt ccaacgcgca gctgggcgat gaagtaaaaa
     5641 tcgagtacaa gcatccgctg ccgaagaaat ttgacctggt gattaccgcc aaagcatacg
     5701 gcaataacgc cagccgtcct attccggtac gcgtaggcaa tgaagaacaa actcttgtgc
     5761 tgggcaatga agtgaccacc accacactgc atttcgataa cccaaccgat gccgacacac
     5821 tggtaattgt gccgccggaa cctgtctcaa ccaacgaagg gaatatcctc ggacactcgc
     5881 cacgtaagct cgggatcggc atggtggaaa ttaaagttgt ggaacgtgaa gggtaatttc
     5941 tgactgatgt gtaggtcgca taaggtgcga cctacacatc ttctccggcc tacaaaacat
     6001 cattgagaaa cagaaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt
     6061 aataatctcg ccttatcaga aagtaattac acccttaatc aactcacgat tgttaatcac
     6121 atcgcgctcg taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc
     6181 ggcagtgatt tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc
     6241 gttgcggctg cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa
     6301 ctcaccttta aacaggccga caaataccac cgtgccgccg tgacgaatca aattcacggt
     6361 gttattcatc gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc
     6421 aaactgcgcc cgtagctgcg cgtcaaaatc ttcggctgac gggtccagta aaggtaattc
     6481 cagacgcgtt gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc
     6541 gccatcggct ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag
     6601 cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg
     6661 ttcaatcaat gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg
     6721 cacgctcaaa tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc
     6781 acagcaattg gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat
     6841 cacagcaact tgctgaccat ttttaagatc agcaatattt ttacccagcc caacaatctc
     6901 cccacatatt tcatggccta aaacacgtgg ataactaaaa aatggttgat ttccacccca
     6961 ggcatgaata tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc
     7021 acccggaata ggtatctcgc gttgtttcca gactaattct ttcggctgct ggcaaattaa
     7081 aacattcatc gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa
     7141 atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt
     7201 taaatcgggt tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc
     7261 cacaatgtga ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt
     7321 ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc gcaccacagt gcgtcatatt
     7381 ctcagccact tacgcgaatg cggcgtcttg acgcaggttg gaaacgacta tgttattgcg
     7441 cgtaaacctg accatgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac
     7501 aaagtgtttg agcaggcttt tttcacgatg ataaaccagc gccagttacg cccaggggaa
     7561 acgttttctg aactgcaact ggctcgggcc gcgggtgtca gcccggttgt agtaagagaa
     7621 tatcttttaa aattcggccg ttataatctt attcacagcg aaaagcgcgg ccagtggagc
     7681 atgaagcaat tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa
     7741 acacattcac ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg
     7801 aaaaccatgc tcgaacgtca tcggctactg cgtgacaaca tcggcaatag ctttcgcatg
     7861 ttctcgcaac ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt
     7921 tttgatcaat cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa
     7981 agcgatctta aacagcgcaa tatcattgcc gtcgatgagc atatgaccat tctcagcgct
     8041 ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc gtaaccattt gaattcagct
     8101 aaacaatcaa tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt
     8161 aaaaacagca aactattagc gggtgcacgg ttttatcaaa agataaatac cgggaaattc
     8221 ccgcttacct atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc
     8281 ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat tcacaattac gtaaacaatt
     8341 aaaattataa atacaggagt ccggcgtgga aaaagaaaat atcaccctcg atccgcgttc
     8401 ttcatttact ccatcttcgt cggcagatat tcccgtgcca ccagatggat tagttcaacg
     8461 cagtacccga attaaacgca ttcaaaccac cgccatgttg ttattatttt ttgcggcggt
     8521 aatcaattat ctcaaccgca gttcgctgtc ggtagcaaat ttaacgattc gtgaagaatt
     8581 gggattaagt gccaccgaaa tcggcgcttt gctctccgtg ttttcactcg cttacggcat
     8641 tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc ccacgcctga tgctgggact
     8701 ggggatgttc ttctggtcac tgttccaggc catgtctggc atggtgcaca gctttacgca
     8761 gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg ccgatgaacc catgcggtgt
     8821 aaaagtcatt aacgactggt tcaacatcaa agagcgcgga cgcccgatgg gcttcttcaa
     8881 cgcagcttct accattggcg ttgccgtaag cccaccgatt ctggcggcga tgatgctggt
     8941 gatgggctgg cgcgggatgt ttattaccat tggtgtactg gggatttttc tcgccatcgg
     9001 ctggtatatg ctctatcgca accgcgagca cgtagaactg actgccgttg agcaagctta
     9061 tctcaatgca ggtagcgtca atgcccgccg agatccgctc agttttgccg aatggcgcag
     9121 cctgttccgt aaccgtacaa tgtggggaat gatgctcgga ttcagtggca tcaactacac
     9181 tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa acagcctata acctggattt
     9241 aaaaagcaca gggtcgatgg cggctatccc tttcctgttt ggggctgccg ggatgctggt
     9301 caacggttac gttactgact ggctggtcaa agggggaatg gctccgatta aaagccgtaa
     9361 gatctgcatt attgccggga tgttctgttc tgccgccttt acgctgatag taccacaagc
     9421 gacaacatcc atgacggcgg ttctgctgat tggcatggca ctgttctgta ttcactttgc
     9481 cggaacatcc tgctggggct tgatccacgt cgcagttgct tctcgcatga ctgcgtcggt
     9541 gggcagtatc cagaactttg ccagcttcat ctgcgcctct tttgcgccga tcattactgg
     9601 ttttattgtt gataccaccc actcattccg tctggcacta atcatctgcg gttgcgtcac
     9661 cgcagcgggg gcactggcgt acatcttcct ggttcgtcag ccgatcaacg acccacgtaa
     9721 agattaatct ccttatgccc gataacattt tgcttatcgg gcattttcat ggcgattaaa
     9781 atgtttccca gttctcctcg ctatctgcca cgaccatttt acgcggcgca gctggcgtca
     9841 cggtttttac cacagccgat gtttcacgct gctgttgctg ctgttgctga atccggaaca
     9901 ctgccacagc ttcggtcagg cgactggcct gctcttccag cgcggcggcg gcagcggcag
     9961 attcttccac cagcgcggcg ttctgttgag ttacccggtc catctcagca accgctaagc
    10021 caacctgatc gataccacgg ctctgctcat cagaagcaga agcaatttcg cccataatgt
    10081 ccgtcacgcg ggtcacggcg ctgacaatct ccgccattgt ttccccggcg ctttcgacca
    10141 gcgtagagcc aacatccact ttccccaccg agtcttcaat caggctttta atttcacgag
    10201 ccgcctgggc gctgcgctgg gccagattac gcacttctcc cgcgaccacc gcaaaaccgc
    10261 gcccttgctc acccgcacgc gcagcctcaa ccgccgcgtt caaagccaga atattggtct
    10321 ggaaggcaat gccgtcaatt acgctgataa tatcggcgat tttctgcgaa ctggtggaga
    10381 tatcgcgcat agtctgcacc acgttatcta ccactttgcc gccgcgttgc gccgtttcag
    10441 aagcacttaa cgccagatgg ctggcctggc gcgcattttc ggcgttctgc ttcaccgttg
    10501 cggtcagttg ctccatgctg gctgccgtct cttccagcga agcggcctgt tgctcggtgc
    10561 gcgaagagag atcgttattg ccggtggcga tctcgctggc accgctatag atggcattgg
    10621 ccccgttgcg cacatcaccg acggtacgca tcagctctcc ctgcatatgg cgcaaactct
    10681 ctgccagttg ccccatctca ttagagccat ccacctcaat cggtttcacc agatcgccgc
    10741 ctgcaatatg acgaatgctg tcaatcaggc gattcattgg cgctaccagc gaggatttaa
    10801 taccgaacca aacggcgaag atgaccgcca gtacgacgat catcacgccc accagaatcc
    10861 acatcgcctg gctgtaggag gcattgttat cgctgacggc gatatcatag agccgatcgt
    10921 tttgctccat gtaagccaca tactgctttt cgaaaccgtc ctgatatccc tgggtcggct
    10981 gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg gatcagctcc gccagcgcat
    11041 tgtgataaat atcgtaatta cgtttgatct ctgccgctgc ggcggtgctc tgacgcgggt
    11101 cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc ctgtttcagc gaaatactgg
    11161 cactctgcat cagctcagca acggttgaac cgctaccaat attattctga tccatcatgt
    11221 agcggatacc cgcgcggttg agggtgttac gcgtctgcaa caacgcgacc cagctgccat
    11281 tcagcgtgga ttgctgctgg cgaatggttt gtaaaacagt gaaattttct ttgtcattct
    11341 ttaaggcatt aaagaacaga ccgcctgatg tcagttgtaa aaggccaaaa acggccaaaa
    11401 ccagcagtaa gctggtcaca attttgatac gttttaacat ggtttctctt tcctgtggac
    11461 cgatgcaaga ttttcggcct ggaaaggaaa aactttatga atatcccgcg gagattacgc
    11521 cga
//
LOCUS       DAJSKJ010000124        11185 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000124 DAJSKJ010000000
VERSION     DAJSKJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.125"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            171..752
                     /gene="mdaB"
                     /locus_tag="ON345_002832"
     CDS             171..752
                     /gene="mdaB"
                     /locus_tag="ON345_002832"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR3072867.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            783..1097
                     /locus_tag="ON345_002833"
     CDS             783..1097
                     /locus_tag="ON345_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR3072868.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1145..3037)
                     /gene="parE"
                     /locus_tag="ON345_002834"
     CDS             complement(1145..3037)
                     /gene="parE"
                     /locus_tag="ON345_002834"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR3072869.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3066..3647)
                     /gene="yqiA"
                     /locus_tag="ON345_002835"
     CDS             complement(3066..3647)
                     /gene="yqiA"
                     /locus_tag="ON345_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR3072870.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3647..4474)
                     /gene="cpdA"
                     /locus_tag="ON345_002836"
     CDS             complement(3647..4474)
                     /gene="cpdA"
                     /locus_tag="ON345_002836"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR3072871.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4499..4921)
                     /locus_tag="ON345_002837"
     CDS             complement(4499..4921)
                     /locus_tag="ON345_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR3072872.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4922..5551)
                     /gene="nudF"
                     /locus_tag="ON345_002838"
     CDS             complement(4922..5551)
                     /gene="nudF"
                     /locus_tag="ON345_002838"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR3072873.1"
                     /translation="MLKPDNLPVTLGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5756..7237
                     /gene="tolC"
                     /locus_tag="ON345_002839"
     CDS             5756..7237
                     /gene="tolC"
                     /locus_tag="ON345_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR3072874.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7385..8056
                     /locus_tag="ON345_002840"
     CDS             7385..8056
                     /locus_tag="ON345_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR3072875.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8062..9222
                     /locus_tag="ON345_002841"
     CDS             8062..9222
                     /locus_tag="ON345_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR3072876.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9260..10048)
                     /gene="ygiD"
                     /locus_tag="ON345_002842"
     CDS             complement(9260..10048)
                     /gene="ygiD"
                     /locus_tag="ON345_002842"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR3072877.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10191..10964
                     /gene="zupT"
                     /locus_tag="ON345_002843"
     CDS             10191..10964
                     /gene="zupT"
                     /locus_tag="ON345_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR3072878.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
BASE COUNT         2755 a         3019 c         2791 g         2620 t
ORIGIN      
        1 aagttgattt cagtattgtc attttcagaa aatatttatc ctgcatcggt gagtcagagc
       61 aagatcagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac
      121 cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggttaaaaa atgagcaata
      181 tcctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg aacgacaccc
      241 tgaccgaagt cgcggatggc acactgcgcg acctcgggca tgatgtccgc atcgttcgcg
      301 ccgacagcga ctacgatgtc aaagcggaag tacaaaactt tctctgggct gatgtggtga
      361 tctggcagat gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa tatattgatg
      421 atgtattcac cgaaggtcat ggaacgctgt atgccagcga tggccgtacc cgcaaagatc
      481 cgtcaaaaaa atacggttcc ggcggcctgg tacagggcaa aaaatatatg ctttctctga
      541 cctggaacgc accaatggaa gccttcaccg aaaaagatca attcttccac ggcgttggcg
      601 tcgacggtgt gtatctgccg ttccataaag caaaccaatt ccttggcatg gaaccgctgc
      661 cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat actgaagaat
      721 atcgcaagca tcttgtggaa atttttggtt aactagagct caggctttag aaggagttaa
      781 ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg
      841 tattggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt tgccacggct
      901 atgcgccaat ggtggattgc gctgctggcg tgagtttcca gtctatggca ccggattcta
      961 tcgtgatgat tgagcagtgg gaaagcatcg cgcatcttga agcgcatctg caaaccccgc
     1021 acatgaaggc gtatagcgaa gccgtaaaag gtgacgtgct ggagatgaat attcgtattc
     1081 tgcagccagg gatttaaacc tgccttgttt gcccggccat cctgaccggg caatgttctt
     1141 tcctttaaac ctcaatctcc gccatgtcgc ctttctcttg caaccaattg cggcgatctt
     1201 ccgagcgttt cttcgccagc agcatatcca tcatcgcgtc agtacgctga tcgtcttcat
     1261 catcgatagt cagctgcacc agacggcgag tgttcggatc aagcgtggtt tcgcgcaatt
     1321 gcatcgggtt catttccccc agacctttaa aacgctggac gttcggcttg cctttcttgc
     1381 gttttaattg ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa taaacctctt
     1441 tcccgagatc aatacggtag agcggtggca gtgcgacgta aacgtgaccg tgtttcacca
     1501 acgcgcggaa atgttttacg aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag
     1561 agtccgcatc cgcgaggata cagattttgc cataacgaag ctggctcaga tcgtcgctgt
     1621 caggatcgat accgatcgct accgaaatat cgtgcacttc ctgcgaagcc agcacttcgt
     1681 cggaagagac ttcccaggtg ttaaggatct tacctttcag cggcatgatc gcctgatatt
     1741 cgcgatcgcg cgcctgcttg gcagatccgc ctgcggagtc gccttccaca aggaacagtt
     1801 cggtacggtt aagatcctgc gcggtacagt cagccagttt gccaggcagc gccgggccgc
     1861 tggtcagctt tttacgcacc acttttttag ccgcacgcat acgacgctgg gcgctggaaa
     1921 tcgccatctc cgccagcagc tccgccgcct gaacgttctg gttcagccac aggatgaagg
     1981 catctttcac cacgcccgaa acgaatgccg cacactgacg cgaagagaga cgctctttgg
     2041 tctgcccggc aaactgcgga tcctgcattt tcaccgacag aacataggcg cagcgatccc
     2101 agatatcttc cgccgacagc ttcacgccgc gcggcagaat gttgcggtat tcacagaact
     2161 cacgcatcgc gtccaacagg ccctgacgca gaccattaac atgggtaccg ccctgcatcg
     2221 ttgggataag gttgacgtag ctttcggtca gcagttcacc gccttccggc agccacagta
     2281 gcgcccagtc cacagcttca gtatcgccag cgaaattacc gataaacggt ttttctggca
     2341 gcgtcggtaa accgtttacc gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc
     2401 agcgctgttc ggtgttgttg atctcatctt taaaggtaat ctcaacgccg gggcacagta
     2461 ctgctttggc cttcagcaca tgcgtcaggc gtgaaacaga aaaacgcggg ctgtcaaaga
     2521 aggtttcatc cggccagaag tgcacgctgg taccggtatt gcgtttaccg caagtgccga
     2581 caacctgtaa atcctgcacc ttttcgccat tttcaaaggc gatgttataa acctgaccat
     2641 cgcggcgcac gttaacttct acgcgcttcg acagggcgtt aaccaccgaa atccccacgc
     2701 catgcaggcc gccagagaac tggtaatttt tgttagagaa tttaccgcct gcatgcagac
     2761 ggcaaagaat cagttcaacc gccggtacac cctcttccgg gtgaatatcc accggcatcc
     2821 cgcgcccatc gtcaataact tctaacgact ggtcagcatg taagattaca tccacgcgtt
     2881 ttgcgtgacc cgccagtgct tcatcgacac tgttatcaat aacttcttgc cccaaatggt
     2941 tagggcgagt ggtatcggta tacatccccg gacggcggcg aaccggctca agcccggtga
     3001 gtacctcaat ggcatcagcg ttataagttt gcgtcatggt ttaagttagc aattcgagtt
     3061 gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg ggttgaaata atcttcgaag
     3121 cccgtgaatg cgtggttgcc gccttctatg acagtctggc ggcaggaagc gtagtacgcc
     3181 accgcctggc ggtaatccag cacttcatct cccgtctgtt gcagcagcca gatcaaatcc
     3241 ggcgcttcca gcgggtcaat ctgcatgact ttgagatcgt aaatatggcg tgactctagc
     3301 acatattgct gcccggtgta ggggttctcg ttctgaccga gatagtccgt cagcagttca
     3361 aacgggcgca ccgccgggtt taccaccact gcgggcagca taaaacattg tgacaaccag
     3421 gtggcgtaat atccccccag tgacgaaccg acaataccca gcgaatcgcc gccatgttcc
     3481 aggacaatgg attccagcag ctctgccgcg tcggaaggat acggcggcaa ctgcggaatg
     3541 atcatctcaa cgtcagggtg atgttccgcc agccagtttt ttaacaagct cgcttttgca
     3601 gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg tagacatcag tagccttctg
     3661 aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc
     3721 catcagcatg taactcgaga gtacgccagc cgggcgcgat agtatccagc gtaaagttgg
     3781 aacagtgcgg cttaaactgc acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc
     3841 agtcgagatc cagctcctga tgaatatgac cgcacagcaa gtatttgacg tgcggaaact
     3901 tcgccagcac ggtatccagt tcgcccgcgt tacgcaggct gtgttgatca agccaactac
     3961 aacccgcagg tagcggatga tgatgcagca gcagcaacgt atggcgttct ggcgcatcgg
     4021 ccagtttacg ttccagccac tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa
     4081 acacctggct atccagcaac aggatttgcc attgctcacc aataaacacc cgcttcgccg
     4141 gggagatacc cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg
     4201 gcagccagac gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc tgataggccg
     4261 cagaggattg atcctgcgct aaatcacctg tcgcgacaat caggtcgaat tcgtgctgat
     4321 gtggacgaat cgcctccagc accgcctggt agctttccca ggtgtttacc cctaacaggg
     4381 cttcgtgctt ttgtgcaaac aggtgagtat cggtaatttg taaaatcctg actctggcct
     4441 caccagccag aggaagggtt aacaggcttt ccaaatggtg tccttaggtt tcacgacgct
     4501 aataaaccgg aatcgccatc gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa
     4561 actgattaat ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga taatcatacc
     4621 gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc agccaccatc gcgtcatgat
     4681 acagacgcac cgtcattgac ggaaggctcc agtaactgat cgcgggcgca gtctgttcta
     4741 ttgtcactag ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg
     4801 ccacctgata gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt
     4861 gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca
     4921 tttatgccca ctcatttttt aacgcttgat gatgcagctg cagccattgc aaagcgatga
     4981 ccgacgctgc gttgtcgatt ttcccctctt ctacccactg gtatgcctgt tcccggctta
     5041 ccacatgaac gcgaatatct tcgttttcat cagccagacc atgaataccg cttgcggtcg
     5101 tggcgtccac ttcgcccacc ataattgacg aacgctcact ggtgcctccc gggcttgcaa
     5161 ggaaacttaa caccggtttg gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc
     5221 gacgggcaac atcttccaca ctttcaccct cttcaatcat cccggcaacc atctccagta
     5281 gccagggggt ttcactggtg tcgtatgcgg caatccgaat ctgctcaatc agcacaactt
     5341 catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc
     5401 gccgtacctc atgactcatt tgcccgttga ataaacgatg acgaaatcta taaagatcta
     5461 atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat ttctacatcg tttttgccaa
     5521 gtgtaacggg caggttgtct ggcttaagca ttgttaatgt cctggcacta atagtgaatt
     5581 aaatgtgaat ttcagcgacg tttgtctgcc gtttgagcag tcatgtgtta aattgaggca
     5641 cattaacgcc ctatggcacg taacgccaac cttttgcggt agcggcttct gctagaatcc
     5701 gcaataattt tacagtttga tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa
     5761 gaaattgctc cccattctta tcggcctgag cctttctggg ttcagttcgt tgagccaggc
     5821 cgagaacctg atgcaagttt atcagcaagc acgccttagt aacccggaat tgcgtaagtc
     5881 tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc
     5941 acagctaggt ttaggtgcag attacaccta tagcaacggc taccgcgacg cgaacggcat
     6001 caactcgaac gcgaccagtg cgtccctgca gttaactcaa tccatttttg atatgtcgaa
     6061 atggcgtgcg ttaacgctgc aggaaaaagc agcagggatt caggacgtca catatcagac
     6121 cgatcagcaa accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat
     6181 tgacgttctt tcctatacac aggcacaaaa agaagcgatc taccgtcaat tagatcaaac
     6241 cacccaacgt tttaacgtgg gcctggtagc gatcaccgac gtgcagaacg cccgcgcgca
     6301 gtacgatacc gtgctggcga acgaagtgac cgcacgtaat aaccttgata acgcggtaga
     6361 gcagctgcgc cagatcaccg gtaactacta tccggaactg gcggcgctga atgtcgaaaa
     6421 ctttaaaacc gacaaaccac agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa
     6481 cctgtcgctg ttacaggcac gcttgagcca ggacctggcg cgcgagcaaa ttcgccaggc
     6541 gcaggatggt cacttaccga cgctggattt aacggcttct accgggattt ctgacacctc
     6601 ttatagcggt tcgaaaactc gtggtgccgc tggtacccag tatgacgaca gcaatatggg
     6661 ccagaacaaa gtgggcctga gcttctcgct gccgatttat cagggcggaa tggttaactc
     6721 gcaggtgaaa caggcccagt acaactttgt tggtgccagc gagcaactgg aaagcgcgca
     6781 tcgtagcgtc gtgcaaaccg tacgttcctc cttcaacaac attaatgcat ctatcagtag
     6841 cattaacgcc tacaaacaag ccgtagtttc cgctcaaagc tcattagacg cgatggaagc
     6901 gggctactcg gtcggtacgc gtaccattgt tgatgtgttg gatgcaacca ccacgctgta
     6961 caacgctaag caagagctgg caaatgcgcg ttataactac ctgattaatc agctgaatat
     7021 taagtcagcc ctgggtacgt tgaacgagca ggatctgctg gcactgaaca atgcgctgag
     7081 caaaccggtt tccactaatc cggaaaacgt tgccccgcaa acgccggaac agaatgctat
     7141 tgctgatggt tatgcgcctg atagcccggc acccgtcgtt cagcaaacat ccgcacgcac
     7201 taccaccagt aacggtcata accctttccg taactgatga cgacgacggg gcttcggccc
     7261 cgtctgaacg taagacaacg taaagatacg ggttatctgc cgcattcttc ccccttctcg
     7321 cttcaatttc gaccagccat cctctattct gatgggtatt taccactggt cccggaagac
     7381 aaaaatgaaa cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg
     7441 ccatctgaca ccagtcgctc tcgcggttgc cactgttttt atgctggctg gctgtgaaaa
     7501 gagtgatgaa acagtgtctc tctatcaaaa tgctgacgac tgttcagctg caaaccctgg
     7561 caaaagcgcc gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc
     7621 gccgaaatac gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc agtgccagca
     7681 ggcaccagcc caggctggca tggcaccaga aaaccaggcg caggcccagc aatccagcgg
     7741 gagtttctgg atgccgctga tggccggtta catgatgggg cgtctgatgg gcggcggcgc
     7801 gggatttgca cagcagccgc tattctcctc gaaaaaccca gccagcccgg cttacggtaa
     7861 atataccgac gcgacgggta aaaactatgg cgcagcccag ccaggccgca ccatgaccgt
     7921 accgaagaca gccatggcac caaaaccggc gaccaccact accgttaccc gtggcggttt
     7981 tggtgaatct gttgccaaac aaagcactat gcagcgtagc gcaactggca cctcttctcg
     8041 ttcaatgggt ggctgatacc gatggaaaga gtcagtatta ccgagcgccc ggactggcgt
     8101 gagaaagccc acgaatacgg tttcaatttt cacaccatgt acggcgagcc gtactggtgt
     8161 gaagatgctt actacaagtt gaccctcgcc caggttgaaa agctggaaga agtcaccgcc
     8221 gaactgcacc agatgtgcct gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg
     8281 accaaattcc gcattccaaa acacacctgg agttttgtgc gccagtcatg gctgacgcac
     8341 cagccatcgc tttattcgcg tcttgatctg gcgtgggatg gcactggcga acctaaactt
     8401 ctggaaaata acgccgatac gccaacgtca ctatacgagg cggcgttctt tcagtggatc
     8461 tggctggaag atcagcttaa cgccggtaac ttgccggagg gcagcgacca gtttaacagt
     8521 ctgcaagaaa aactgatcga tcgcttcgtt gagctgcgtg aacagtatgg cttccagttg
     8581 ctgcatctca cctgctgtcg cgacacggtg gaagatcgcg gaaccattca gtatttgcag
     8641 gactgcgcaa cggaagctga aattgctact gagttcctct acatcgatga tatcgggtta
     8701 ggtgaaaaag gtcagttcac ggatttacag gatcaggtaa tttccaacct gttcaagctg
     8761 tatccgtggg aatttatgtt gcgtgagatg ttttccacca agctggagga tgcaggcgta
     8821 cgctggctgg aaccggcgtg gaagagcatt atctccaaca aggcacttct accgctactg
     8881 tgggagatgt tcccgaatca cccgaacctg ctgcccgctt attttgcgga agatgatcat
     8941 ccgcaaatgg aaaaatatgt ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg
     9001 atcattgaaa acggcaaaac cattgaagca gcggaaggtc cgtatggcga agaagggatg
     9061 attgttcagc aattccaccc gttaccgaaa ttcggcgaca gctatatgct gattggtagc
     9121 tggctggtga acgatcaacc cgccggaatt ggcattcgtg aagaccgtgc attgatcacc
     9181 caggatatgt ctcggtttta tccacatatt tttgttgagt aatcaatgat gccggatggc
     9241 attcgccatc cggtaattgt taacctatct gcaccgacag catactcagg ctgcccattt
     9301 ctataccatc gaccggaatg gtaattggct cctgcccatc ccacgcacct aagacataca
     9361 acaacggcag atagtgctcc ggcgttgggt tcgataacgc gccaccttca tggtcgaggt
     9421 aattcaccag aggatgctgc tctaccgccc cttgccacgt cagattcgct ttcacatact
     9481 cattaaacga catcgcccaa ggatatggtg aactatcacc gtgccacttc actgtacgca
     9541 ggttatgcac cacgttgcca ctggcgacca acattattcc ttcatctcgc agcgctgcca
     9601 gtttgcgccc catttcgaaa tgccaggcgg caggtttgct actgtcgata ctcaactgca
     9661 ccatcgggat atcggcgtca ggatacatct taatcagcac gccccaggaa ccgtggtcaa
     9721 agccccaggc ttctttatcc agcgtcaccg ggaccggcgc taacagctca accagatgct
     9781 gtgccagcac aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc
     9841 caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa
     9901 accagtgagc cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc cccaacttct
     9961 gccagctgcg ggtatacaaa ttatcttcca gcacgttcat cggactaccg tgacctaaaa
    10021 acaatgctgg catacgtgtt gaagacatga tgatatcctt aactaaaggt gtcattttga
    10081 tatcctcaca atacgcttgt tcggcggagt aagaacccgg ataacaatga tgatgatcat
    10141 cagttatttt gacgatctgc ctgaaggtga agatttataa ggagttgtcg atgtcagtac
    10201 ctctcattct gaccatactg gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc
    10261 tcgggcaaaa accctcgaac cgcttactgg cgttttcgct aggatttgcg gcggggatca
    10321 tgttgctcat ctcattaatg gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc
    10381 ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg ctattttggc ctggaccgca
    10441 tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg ttgccaaaat
    10501 cgatcaagcg cacagccatt ctgctcactc tcggcatcag tctgcataac ttcccggaag
    10561 ggattgccac ctttgtcacg gcgagcagca acctggagct gggatttggc atcgcactgg
    10621 ccgtcgcgtt gcacaatatt cctgaaggtc tggcggttgc aggcccggtt tatgcagcaa
    10681 cgggttctaa acgcacggca attctgtggg cggggatttc tggattggca gaaattcttg
    10741 gtggtgtgct ggcgtggtta atcctcggta gcatgatttc ccccgtggtc atggcggcaa
    10801 tcatggcggc ggttgcagga attatggtgg cgctctcggt tgatgaatta atgccgctcg
    10861 ccaaagagat tgaccctaat aataacccca gctatggcgt actatgtgga atgtcagtga
    10921 tgggattcag tttagtgctg ctacaaacgg cgggaattgg ttaaaagcaa catcagaagt
    10981 tgtccagcgc atggacaact tctgctagct tcactaaagc cttattctac cttttcggct
    11041 gtctcttctc tcgtactgtt taagtatttg ttccgcgctt caagcagtgc gtctctgtcc
    11101 tggcgaaacg cccgacaata acgtttcaaa tgcaggaaat aacccaccac aacaagagca
    11161 atcaatacaa tccagtacca ggcga
//
LOCUS       DAJSKJ010000125        11066 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000125 DAJSKJ010000000
VERSION     DAJSKJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.126"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..549
                     /locus_tag="ON345_002844"
     CDS             <1..549
                     /locus_tag="ON345_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3072879.1"
                     /translation="GEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQ
                     IMKFEVQDASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSH
                     MQKSKFSYIENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSI
                     QLDYSLLYSNIFEFQVKYFFPR"
     gene            complement(594..1493)
                     /gene="eamA"
                     /locus_tag="ON345_002845"
     CDS             complement(594..1493)
                     /gene="eamA"
                     /locus_tag="ON345_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR3072880.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1688..2875
                     /gene="ydeE"
                     /locus_tag="ON345_002846"
     CDS             1688..2875
                     /gene="ydeE"
                     /locus_tag="ON345_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR3072881.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3002..3097
                     /gene="mgtS"
                     /locus_tag="ON345_002847"
     CDS             3002..3097
                     /gene="mgtS"
                     /locus_tag="ON345_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR3072882.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3316..4206)
                     /gene="dgcZ"
                     /locus_tag="ON345_002848"
     CDS             complement(3316..4206)
                     /gene="dgcZ"
                     /locus_tag="ON345_002848"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR3072883.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAVVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4461..4853)
                     /gene="ydeI"
                     /locus_tag="ON345_002849"
     CDS             complement(4461..4853)
                     /gene="ydeI"
                     /locus_tag="ON345_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR3072884.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            5129..5647
                     /locus_tag="ON345_002850"
     CDS             5129..5647
                     /locus_tag="ON345_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR3072885.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(5691..7736)
                     /gene="dcp"
                     /locus_tag="ON345_002851"
     CDS             complement(5691..7736)
                     /gene="dcp"
                     /locus_tag="ON345_002851"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR3072886.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNAILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAHLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRDIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQHRGLNI"
     gene            7873..8619
                     /gene="ydfG"
                     /locus_tag="ON345_002852"
     CDS             7873..8619
                     /gene="ydfG"
                     /locus_tag="ON345_002852"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR3072887.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            8708..9394
                     /gene="ydfH"
                     /locus_tag="ON345_002853"
     CDS             8708..9394
                     /gene="ydfH"
                     /locus_tag="ON345_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR3072888.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            9572..9775
                     /gene="ydfZ"
                     /locus_tag="ON345_002854"
     CDS             9572..9775
                     /gene="ydfZ"
                     /locus_tag="ON345_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR3072889.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(9811..>11066)
                     /locus_tag="ON345_002855"
     CDS             complement(9811..>11066)
                     /locus_tag="ON345_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3072890.1"
                     /translation="QQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLAAMC
                     EPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKA
                     AGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMVDRIVPAV
                     TEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEM
                     KLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVDLQD
                     YANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWM
                     RYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFT
                     AKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2934 a         2753 c         2507 g         2872 t
ORIGIN      
        1 ggcgaaggaa ttattcacag aaagcatggt tcaggaacat acattcaaaa aaagaatttt
       61 gttgcacaca ttgatcacat gaacagtttc agtgaaattg cgcataaaag cggcaaagag
      121 gcagggagtc agattatgaa atttgaagtg caggatgctt cacccactat tgcgactgag
      181 ctgaatttag tgactggtga gcaggtttat tacataaaga gactgcgatt cattgaggat
      241 aatgcagcac aattagaaga aacgtggatg tcagtagcac gttttcctga tttaaccgtg
      301 tcacatatgc aaaaatccaa attttcatat attgagaatg aatgcgggat caaaatcatt
      361 ggcacctttg aaactttctc ccctactttt cctaccccag aaatcgccag tattttacgg
      421 atcagcccac gggatcccat acttaaaatt cagacccagg ctgtggacag taactctatt
      481 cagctggatt attcgttact ttacagcaat attttcgagt tccaggtaaa gtactttttc
      541 ccgcgataaa agcagacgca cagagtgctg acatcacatt ctgtgcgtat ggtttagcct
      601 ctaaccttta ccgctttacg ccaccgcaag ccaaatacat tgatatacag cccggtcata
      661 atgagcaccg cacctaaaaa ttgcagaccc gttaagcgtt catccaacaa tagtgccgca
      721 cttgccagtc cgactacggg caccagtaac gataacggtg caacccgcca ggtttcatag
      781 cgtcccagta acgtccccca gatcccataa ccaacaattg tcgccacaaa cgccagatac
      841 atcagcgaca agatggtggt catatcaata gtaaccagac tgtgaatcat ggatgcggaa
      901 ccatcgagaa tcagcgaggc aacaaagaag ggaatgattg ggattaaagc gctccagatt
      961 accagcgaca tcaccgccgg acgcgttgag tgcgacatga tctttttatt gaagatgttg
     1021 ccacacgccc aactaaatgc tgcagccagg gtcaacataa agccgagcat cgccacatgc
     1081 tgaccgttca gactatcttc gattaacacc agtacgccaa aaatcgctaa ggcgatcccc
     1141 gccaattgtt tgccatgcag tcgctccccg aaagtaaacg cgccaagcac gatagtaaaa
     1201 aacgcctgtg cctgtaacac cagcgaagcc agtccagcag gcataccaaa gttaatggcg
     1261 cagaacagaa aggcaaactg cgcaaaactg atggttaatc cataccccaa gagcaaattc
     1321 agtggtactt tcggtcgtgc aacaaaaaag atggccggaa aagcgaccag cataaagcgc
     1381 aaaccggcca gcatcagcgg tggcatgtta tgaagcccca ctttgatgac cacaaaattt
     1441 agcccccata cgaccactac cagtagcgcc aacaccccat cttttcgcga cattctaccg
     1501 cctctgaatt tcatcttttg taagcaatca acttagctga atttactttt ctttaacagt
     1561 tgattcgtta gtcgccggtt acgacggcat taatgcgcaa ataagtcgct atacttcgga
     1621 tttttgccat gctatttctt tacatctcta aaacaaaaca taacgaaacg cactgccgga
     1681 cagacaaatg aacttatccc tacgacgctc taccagcgcc cttcttgcct cgtcgttgtt
     1741 attaaccatc ggacgcggcg ctacgctgcc atttatgacc atttacttga gtcgccagta
     1801 cagcctgagt gtcgatctaa tcggttatgc gatgacaatt gcgctcacta ttggcgtcgt
     1861 ttttagcctc ggttttggta tcctggcgga taagttcgac aagaaacgct atatgttact
     1921 ggcaattacc gccttcgcca gcggttttat tgccattcct ttagtgaata acgtgacgct
     1981 ggtggtgctc ttttttgccc tcattaactg cgcctattct gtttttgcta ccgtgctgaa
     2041 agcctggttt gccgacaatc tttcgtccac cagcaaaacg aaaatcttct caatcaacta
     2101 caccatgcta aacattggct ggaccatcgg tccgccgctc ggcacgctgt tggtgatgca
     2161 gagcatcaat ctgcccttct ggctggcagc tatctgttcc gcgtttccca tgcttttcat
     2221 tcaaatttgg gtaaagcgca gcgagaaaat catcgccacg gaaacaggca gtgtctggtc
     2281 gccgaaagtt ttattacaag ataaagcact gttgtggttt acctgctctg gttttctggc
     2341 ttcttttgta agcggcgcat ttgcttcatg catttcacaa tatgtgatgg tgattgctga
     2401 tggggatttt gccgaaaagg tggtcgcggt tgttcttccg gtgaatgctg ccatggtggt
     2461 tacgttgcaa tattccgtgg gccgccgact taacccggct aacatccgcg cgctgatgac
     2521 agcaggcacc ctctgtttcg tcatcggtct ggtcggtttt attttttccg gcaacagcct
     2581 gctattgtgg ggtatgtcag ctgcggtatt tactgtcggt gaaatcattt atgcgccggg
     2641 cgagtatatg ttgattgacc atattgcgcc gccaggaatg aaagccagct atttttccgc
     2701 ccagtcttta ggctggcttg gtgccgcgat taacccatta gtgagtggcg tagtgctaac
     2761 cagcctgccg ccttcctcgc tgtttgtcat cttagcgttg gtgatcattg ctgcgtgggt
     2821 gctgatgtta aaagggattc gagcaagacc gtgggggcag cccgcgcttt gttgatttaa
     2881 gtcgaacaca ataaagattt aattcagcct tcgtttaggt tacctctgct aatatctttc
     2941 tcattgagat gaaaattaag gtaagcgagg aaacacacca caccataaac ggaggcaaat
     3001 aatgctgggt aatatgaatg tttttatggc cgtactggga ataattttat tttctggttt
     3061 tctggccgcg tatttcagcc acaaatggga tgactaatga acggagataa tccctcacct
     3121 aaccggcccc ttgttacagt tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa
     3181 ccgccagtaa accggcggtg aatgcttgca tggatagatt tgtgttttgc ttttacgcta
     3241 acaggcattt tcctgcactg ataacgaatc gttgacacag tagcatcagt tttctcaatg
     3301 aatgttaaac ggagcttaaa ctcggttaat cacattttgt tcgtcaataa acatacagcg
     3361 atttcttccc gtttgcttac cctcatacat tgcccggtct gctcttccaa tgaccacatc
     3421 cagaggctct tcaggaaatg cgcgactgac acctgctgtc acggtaatgt tgatatgccc
     3481 ttcagaatgt gtgatggcat ggttatcgac taactggcaa attctgacac ctgcacgaca
     3541 tgcttcttca tcattagccg ctttgacaat aatgataaat tcttcgcccc cgtagcgata
     3601 aaccgtttcg taatcacgcg tccaactggc taagtaagtt gccagggtgc gtaatactac
     3661 atcgccgatt aaatgcccgt aggtatcatt aaccaattta aatcggtcaa tatccaacaa
     3721 cattaaataa agattcagag gatcagtgtt gcgtaactga tgatcaaagg attcatcaag
     3781 aacccgacgc cccggcaatc ccgtcaaaac atccatattg ctacggatcg tcagcaaata
     3841 aattttgtaa tcggttaatg ccgcagtaaa agaaagcaac ccctcctgaa aggagtcgaa
     3901 atgcgcgtcc tgccagtgat tttcaacaac agccagcatt aattcccgac cacagttatg
     3961 catatgttga tgggcagaat ccattagccg aacgtaaggt aattcatcgt tatcgagtgg
     4021 ccccagatga tcaatccacc gaccaaactg gcacagtcca taagaatggt tatccgttat
     4081 ttctggctta ctggcatctc tcgcgaccac gctgtgaaac atactcacca gccactggta
     4141 gtgggcatcg atagccttat tgagatttaa caagatggca tcaatttccg ttgtcttctt
     4201 gatcattgcc actccttttt cacagttcct tgtgcgcgct attctaacga gagaaaagca
     4261 aaattacgtc aatattttca tagaaatccg aagttatgag tcatctctga gataacattg
     4321 tgatttaaaa caaaatcagc ggataaaaaa gtgtttaatt ctgtaaatca cctctgcatt
     4381 atcgtaaata aaaggatgac aaatagcata acccaatacc ctaatggccc agtagttcag
     4441 gccatcaggc taatttattt ttatttctgc aaatgagtga cccgaacgac ggccggcgcg
     4501 cttttcttat ccagactgcc gctaatgttg atcatctggt ccggctgaac ttctcgtcca
     4561 tcaaagacgg ccgcaggaat aacgacatta atttcaccgc tcttatcgcg aaaaacgtaa
     4621 cggtcctctc ctttgtgaga aatcaaatta ccgcgtagtg aaaccgaagc gccatcgtgc
     4681 atgttttttg cgaaatcaac ggtcattttt tttgcatcat cggttccgcg atagccatct
     4741 tctattgcat gaggcggcgg tggcgctgca tcctgtttta aaccgccctg gtcatctgcc
     4801 aacgcataag gcatgacaag aaaacttgct aatacaatgg cctgaaattt catactgact
     4861 ccttaattgc gtttggtttg acttattaag tctggttgct atttttataa ttgccaaata
     4921 agaatattgc caattgttat aaggcattta aaatcagcca actagctgtc aaatatacag
     4981 agaatttaac tcactaaagt taagaagatt gaaaagtctt aaacatattt tcagaataat
     5041 cggatttata tgtttgaaaa ttattatatt ggacgagcat acagaaaaag caaatcacct
     5101 ttacatataa aagcgtggac aaaaaacagt gaacattaat agagataaaa ttgtacaact
     5161 tgtagatacc gatactattg aaaacctgac atccgcgttg agtcaaagac ttatcgcgga
     5221 tcaattacgc ttaactaccg ccgaatcatg caccggcggt aagttggcta gcgccctgtg
     5281 tgcagctgaa gatacaccca aattttacgg tgcaggcttt gttactttca ccgatcaggc
     5341 aaagatgaaa atcctcagcg taagccagca atctcttgaa cgatattctg cggtgagtga
     5401 gaaagtggca gcagaaatgg caaccggtgc catagagcgt gcggatgctg atgtcagtat
     5461 tgccattacc ggctacggcg gaccggaggg cggtgaagat ggtacgccag cgggtaccgt
     5521 ctggtttgcg tggcatatta aaggccagaa ctacactgcg gttatgcatt ttgctggcga
     5581 ctgcgaaacg gtattagctt tagcggtgag gtttgccctc gcccagctgc tgcaattact
     5641 gctataacca ggctggcctg gcgatatctc aggccagcca ttggtggtgt ttatatgttc
     5701 aagccacgat gttgcagcat cggcataatc ttaggtgcct taccgcgcca ttgtcgatac
     5761 aggcgttcca gatcttcgct gttacctctg gaaaggatcg cctcgcgaaa acgcagccca
     5821 ttttcacgcg ttaatccgcc ctgctcaaca aaccactgat aaccatcatc ggccaacatt
     5881 tgcgtccaca gataagcgta ataacctgca gcatatccgc caccaaaaat atgggcgaaa
     5941 taactgctgc gatagcgtgg cggtatagca ggaagatcca tattttccgc caccagcgcc
     6001 cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt cttccaggca atgccagcgc
     6061 atatcgagaa gtgcggcgct aagcagttcg ctcatctcat accctttgtt gaacaggctg
     6121 gcattacgca ttttctgttg cagttcgtca ggcattgctg ccccgctctg ataatgccgg
     6181 gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt cgttgatttg cgacggaaat
     6241 tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg cataacgctg gcgggcaaaa
     6301 aggccgtgca gcgtatgacc aaattcatgg aataaggtta tgacatcatc ccagagtaac
     6361 aacgcaggct caccggcagc gggtttctga taattgcaaa cgttataaat taccggatgc
     6421 gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc atgcaccgcc gctttttgaa
     6481 tcacgggcga agaaatcacc gtaaaataac gccagtccca cgccattatg atcaaaaatt
     6541 tcccacacac gaacgtcagg atggtagaca ggaatatcaa aacgttcgac aaacttaata
     6601 ccgaagagct gattcgcggt ccagaataca ccttcattca acaccgtgtt taattcaaaa
     6661 tatggcttga gctgcgcctc atcaagatca aatttctccc gccgtacctg ttcggcataa
     6721 aatgcccagt cccacggctg cgcgctaaac ccgccttgct gcttatcgat aaccgcctgt
     6781 atggaggcta attcatcgct agcacgttga cgcgccgctg gaacaatttc ccgcataaag
     6841 ttgagtgctg cttctggcgt ttttgccatc tgatcggcga ttttccatgc ggcataatga
     6901 ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta ccagacgttg aatgatatcg
     6961 cgggtatcat tgccatcatt tttttccgct cgcgtccagc ccgcaataaa cagtttttca
     7021 cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt gggtggtatt cagcagcgga
     7081 atcagccatt tgttatccag acctttctcg cgagccgcct ctgccgccag cgcaatctct
     7141 tgctcactca ttcctgccag atgcgcgata tcgttcacaa ccagaccgcc ggatttattt
     7201 gctgcaagta accgctggtt aaactggctg gtcagagtcg cagcttctgt attcagtact
     7261 tttaattttg ctttatcagc ttgcgcaagt ttggctccgg caaggacaaa acgttgatga
     7321 ataacctcca ccaggcggat ggattcacta tcaagcccca gggattcacg gcgctgccag
     7381 acagcatcta ccctcgcgaa taattcaccg ttcagataga tatcattagc cagttccgcc
     7441 agttcagcgg aaaactgttc atcaagacgc tgtaattcat cattggtatg cgccgcagtc
     7501 atcgcaaaaa agacgctggt aacgcgggta agtaattctc cgctttgttc cagtgccaga
     7561 atagcattgt tgaaatcagg cgtttgcggg ttaagcgcga tggcagcaat ttctgcccgc
     7621 ttttgctgca ttccctcatc gaatgccggg cgatagtgat gattggcaat ttgatcaaaa
     7681 tgaggagcca gatacggcag tgtgctttgc acaaggaaag gattcattgt tgtcattttc
     7741 ttctcctgaa cgcgaggtgt tccatagcgt aggcttactg ataacggagt gcaatcttgc
     7801 aaaccagtat tacccgctct taagcatcac gtgctatgtt attgacacac aaaagcgttg
     7861 aggaacagtg agatgatcgt tttagtaact ggagcaacgg caggttttgg tgaatgcatt
     7921 actcgtcgtt ttattcaaca agggcataaa gttatcgcca ctggccgtcg ccaggagcgg
     7981 ttgcaggagt taaaagacga actgggagat aatctgtata tcgcccaact ggacgttcgc
     8041 aaccgcgccg ctattgaaga gatgctggca tcgctccctg ccgagtggtg caatattgat
     8101 atcctggtaa ataatgccgg cctggcgttg ggcatggagc ctgcgcataa agccagcgtt
     8161 gaagactggg aaacgatgat tgataccaac aacaaaggcc tggtatatat gacgcgcgcc
     8221 gtcttaccgg gtatggttga acgtaatcat ggtcatatta ttaacattgg ctcaacggca
     8281 ggtagctggc cttatgccgg tggtaacgtt tacggtgcga cgaaagcgtt tgttcgtcag
     8341 tttagcctga atctgcgtac ggacctgcat ggtacggcgg tgcgcgtcac cgacatcgaa
     8401 ccgggtctgg tgggtggcac cgagttttcc aatgtccgct ttaaaggcga tgacggtaaa
     8461 gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag aagatgtcag cgaagccgtt
     8521 tggtgggtgt caacgctgcc tgctcacgtc aatatcaata ccctggaaat gatgccggtt
     8581 acccaaagct atgccggact gaatgtccac cgtcagtaat ttttataccc ggcgtaactg
     8641 ccgggttatt gcttgtcaca aaaaagtggt agactcatgc agttaactca ctcacaagca
     8701 agaacgaatg accgtcgaaa cgcaacttaa tcccacacag cctgtcaatc agcaaattta
     8761 tcgtatcctt cgtcgcgaca ttgtccattg cctgattgct ccaggcacac cgttgtcgga
     8821 aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg gttcgtgaag cctttattaa
     8881 actggcggaa aacggcctga ttcaaattcg tccacagcgt ggcagctacg tcaacaaaat
     8941 ttccatggcc caggtgcgca acggcagttt tatccgtcag gccattgagt gcgcggtggc
     9001 gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac caactggaac aaaatcttca
     9061 ccagcaacgc attgccattg agcgcaagca actggatgat ttttttgaac ttgatgacaa
     9121 cttccatcaa ctcctgacgc agattgccga ctgtcaactg gcgtgggata ccattgagaa
     9181 cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc gaccatgttt ctccaccaga
     9241 aatgctgtta cgccagcatc ttgatatttt ctctgccctg caaaaacgtg atggcgatgc
     9301 ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc gaatccgtgc gccagatccg
     9361 ccaggaaaac agcgactggt ttagcgaaga gtaattcatt tactctcatc ccatccggga
     9421 tgagaggctt ttccccccgc cttacggctc atgcatgcat caaaaaagat gtgagcttga
     9481 tcaaaaacaa aaaatatttc actcgacagg agtatttata ttgcgcccgt tacgtgggct
     9541 tcgactgtaa atcagaaagg agaaaacacc tatgacgacc tacgatcgta accgtaacgc
     9601 aatcaccact ggcagccgtg ttatggttag cggcaccggt cacactggca agatcctgtc
     9661 gattgatact gaaggtctga ccgctgagca aatccgccgc ggaaaaaccg tagttgttga
     9721 aggttgtgaa gagaaactgg cacctctgga cctgattcgt ctcggcatga actaagtgtg
     9781 tgaatgccgc cgatggcggc attgcttttt ttacttcacg gaatatttcg ccacggtcgc
     9841 tttcgcgcca tgcgctaata aagacaagta cgcttccgtc acttttgcag taaacaagct
     9901 attgtctggc aaatcatcac caaagattgc cttaatcgcc agcaatgact ggacgcgcgc
     9961 tttcccttcg gcactacttt gtacagcctt ctgaataaca ggtaacagtg ggtcactgat
    10021 ttctatcgga tttccctgtt catcaacacc accgacataa cgcatccaac ccgcgacgcc
    10081 cagcgccagc agatcgaact tgctgtcatg cgccagatgc cagcgaacag aatccaacat
    10141 ccgctgtggc aatttctggc taccatccat cgcaatctgc caggttcgat gacgtaacgc
    10201 cgggttgcta tagcgtgcaa ttaatcggtt agcgtaatct tgcaaatcaa cgccctgcac
    10261 tttcagcgtc ggcgcttgtt cctgcaacat caagccatac gccgcataac gataatgttc
    10321 atcttccatg cagtcattaa tgtgctgata tccagcaaga taacccaaat acgccaggaa
    10381 tgaatgactg ccgttgagca tgcgcaactt catctcttca taaggcagca catcactaac
    10441 cagttcggct cccgcttttt cccattctgg acgtccggca acaaagttat cttctattac
    10501 ccactggcgg aaaggttcac aggcaacgcc cgcaggatcg cccacaccgg taagttgttc
    10561 gattttcgcc agcgtatcct ctgtcactgc gggcacaata cggtccacca ttgttgatgg
    10621 gaaagtcacg ttatcttcga tccattgtgc cagttttaca tcaacagctt tcgcgtatga
    10681 agtgacaacg tcacgcataa catgaccgtt ttctggcatg ttgtcacatg acatgacggt
    10741 aaatgcggga agtcctgccg ctttacggcg ggccagcgcc tcaacaatca cccctgttgc
    10801 tgttttcggc tggtggggat tttgcacgtc ggcagcgacc atcgggtgat cgagcattaa
    10861 ctgtccggtc gccggagagt ggaaataccc tttttcggtg attgtcagag agacaatcgc
    10921 gatttgcggt tcacacatcg cagccaacac ggtttctaag ccatccatct gtacgtgcaa
    10981 ggctttttta acgacgccaa cgacgcgagc cgtccacgca tcggccgaca tttccgcaac
    11041 ggtataaaga ttatcttgct gttgta
//
LOCUS       DAJSKJ010000126        10984 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000126 DAJSKJ010000000
VERSION     DAJSKJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.127"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(74..1903)
                     /gene="glmS"
                     /locus_tag="ON345_002856"
     CDS             complement(74..1903)
                     /gene="glmS"
                     /locus_tag="ON345_002856"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR3072891.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2065..3435)
                     /gene="glmU"
                     /locus_tag="ON345_002857"
     CDS             complement(2065..3435)
                     /gene="glmU"
                     /locus_tag="ON345_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR3072892.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3786..4205)
                     /gene="atpC"
                     /locus_tag="ON345_002858"
     CDS             complement(3786..4205)
                     /gene="atpC"
                     /locus_tag="ON345_002858"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR3072893.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4226..5608)
                     /gene="atpD"
                     /locus_tag="ON345_002859"
     CDS             complement(4226..5608)
                     /gene="atpD"
                     /locus_tag="ON345_002859"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR3072894.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5635..6498)
                     /gene="atpG"
                     /locus_tag="ON345_002860"
     CDS             complement(5635..6498)
                     /gene="atpG"
                     /locus_tag="ON345_002860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR3072895.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6549..8090)
                     /gene="atpA"
                     /locus_tag="ON345_002861"
     CDS             complement(6549..8090)
                     /gene="atpA"
                     /locus_tag="ON345_002861"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR3072896.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8103..8636)
                     /gene="atpH"
                     /locus_tag="ON345_002862"
     CDS             complement(8103..8636)
                     /gene="atpH"
                     /locus_tag="ON345_002862"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR3072897.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8651..9121)
                     /gene="atpF"
                     /locus_tag="ON345_002863"
     CDS             complement(8651..9121)
                     /gene="atpF"
                     /locus_tag="ON345_002863"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR3072898.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9183..9422)
                     /gene="atpE"
                     /locus_tag="ON345_002864"
     CDS             complement(9183..9422)
                     /gene="atpE"
                     /locus_tag="ON345_002864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR3072899.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9469..10284)
                     /gene="atpB"
                     /locus_tag="ON345_002865"
     CDS             complement(9469..10284)
                     /gene="atpB"
                     /locus_tag="ON345_002865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR3072900.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10293..10673)
                     /gene="atpI"
                     /locus_tag="ON345_002866"
     CDS             complement(10293..10673)
                     /gene="atpI"
                     /locus_tag="ON345_002866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR3072901.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2614 a         3004 c         2730 g         2636 t
ORIGIN      
        1 atagatgaat ttattgatgt cagttataaa aaagggccac cggtcggcag ccctttttta
       61 tctatgatag tgattactca accgtaaccg attttgccag gttacgcggc tggtcaacgt
      121 cggtgccttt gatcagcgcg acatggtaag ccagcagctg cagcggaacg gtgtagaaga
      181 tcggtgcaat cacctcttcc acatgcggca tctcgatgat gtgcatgtta tcgctactta
      241 caaaacccgc atcctgatcg gcgaagacat acaactgacc gccacgcgcg cgaacttctt
      301 caatgttgga tttcagtttt tccaacagtt cgttgttcgg tgcaacgacg ataaccggca
      361 tatccgcgtc aatcagcgcc agcgggccgt gtttcagctc gcctgcagcg taggcttcag
      421 cgtgaatgta agagatctct ttcagcttca atgcgccttc cagcgcgatt gggtactgat
      481 cgccacggcc caggaacagc gcgtgatgtt tgtcagagaa atcttctgcc agagcttcaa
      541 tgcgtttgtc ctgagacagc atctgctcaa tacggctcgg cagcgcctgc agaccatgca
      601 caatgtcatg ttcaatggag gcatccagac ctttcaggcg agacagcttc gccaccagca
      661 tcaacagcac agttaactga gtggtgaatg ctttagtgga tgccacgccg atttccgtac
      721 ccgcgttggt catcagcgcc agatcggatt cgcgcaccag agaagaaccc ggaacgttac
      781 agattgccag cgaaccaagg taacccagct ctttcgacag acgcaggccc gccagggtat
      841 ccgcggtttc gccagactgt gacaaggtga tcatcaggct gttacgacgc acggcagatt
      901 tgcgatagcg gaattcagag gcgatttcga cgtcgcacgg aatacctgcc agcgattcaa
      961 accagtaacg ggaaaccata ccggagttat aagaagtacc acaggcgagg atctgaatat
     1021 gctcaacctt cgacagcagt tcgtcggcgt tcggtcccag ctcgcttaaa tcaacctgac
     1081 cgtggctgat gcgcccggta agggtgtttt tgatcgcgtt cggctgttcg tagatctctt
     1141 tctgcatgta gtgacggtaa atacctttat cgcccgcgtc atattgcaga ttggattcga
     1201 tatcctgacg ttttacttcc gcgccagttt tatcgaagat gtttaccgaa cggcgagtaa
     1261 tttccgcaat atcgccctct tcaaggaaga taaagcgacg ggtcaccggc agcagcgcca
     1321 gctggtcaga agcgataaag ttttcgccca tacccagacc aatcaccagc ggactaccag
     1381 aacgtgccgc cagcagggta tccgggtgac gggagtccat gatcactgta ccgtacgcac
     1441 cacgcagttg cgggatagca cgcagaacag cctcacgcag agtaccgccc tgtttcagct
     1501 cccagttcac cagatgggca attacttcgg tgtctgtttc agaaacgaag gtatagccac
     1561 gcgcttttaa ctcttcgcgc agcggttcat ggttttcgat gatgccgtta tgcaccacca
     1621 caatgtgttc agaaacatgc ggatgcgcat tcacttctga aggttcaccg tgggtcgccc
     1681 agcgagtatg agcaataccc gtaccgccat gcagaggatg ttcttccgct gcctgagcca
     1741 gcatctggac tttaccgagg cgacgcaggc gggtcatatg accttccgca tcaacaacgg
     1801 ccagaccggc agagtcatat ccgcggtatt ccagacgacg taaaccttca agaaggattt
     1861 ctgctacatc acgttgcgcg atcgcgccaa caattccaca catagttttt gattccgatt
     1921 tatatcgttg tcggtcaacc tgtatgcccg ttttttcggg cgccccgagc cttgtagaga
     1981 gtggggttat ttttatagtt actgcttgtg ggagggagat tatgttatct cctcatccca
     2041 gatgaccgga ttacccggcc agaattactt tttctttacc ggacgacgcc agccttcttt
     2101 ctgagtctgc ggcacacggc tgatagctaa tgcattttcg ccgacattac gcgtcacagt
     2161 tgtacccgca gcaatggtcg cgcctttgcc tactgttacc ggggccacca gctgagtgtc
     2221 ggaaccgaca aacacatcgt cgccgataat ggtcttaaat ttattcgcac catcgtagtt
     2281 gcaggtaatg gttcccgcgc cgatgttaac gttatcgcca atttccgcat cgcccaggta
     2341 agtcagatga ccagctttcg agcctttacc cagacgcgct tttttcatct caacaaagtt
     2401 accgacgtgt gcaccttcca gcaactcagc accaggacgc agacgggcaa acgggccaat
     2461 agtacaggcc gctgccagat tcgcgtcttc cacaacggta tacggactga tttcgcaatc
     2521 atcgccaatc acgctgtttt taatcacgca accggtgccg attttcacgc gatgaccgag
     2581 agtcacgttg ccctcgatga taacgttagt atcaatttca acatcgcgcc cgtgagtaag
     2641 cgtaccgcgc agatcaaaac gcgccggatc gcgcagcata acgcctgcta acagcagttt
     2701 ttcagcctgt tcggactggt aaacgcgctc cagacgggag agttgcaggc ggttattcac
     2761 accttctact tcgcttaaac gttgcggatg aacggcgacg atttcacgcc cttcctgata
     2821 cgccagcgca ataatgtcgg tgatgtagta ttcgccctga gcattattgt tggtcagctt
     2881 cgccagccag cgtttcatat ctgcgccgtt agcgatcaga atgccggtgt tgatctcctg
     2941 aatctgacgc tgctcgtcgg tggcatcttt gtgctcaaca atgccggtaa ctttgccatt
     3001 ttcacgggtg atacgtccat aaccggtcgg atcatccagt ttcaccgtca gcagaccaat
     3061 gccaccctgc ggtttagcat cacgcaggcg ctggagtgtt tcgacagaga tcagcggcac
     3121 gtcgccgtag agcattaaaa tgtcttcatc atcggcaaag aaaggtgcgg cctgctgcat
     3181 tgcatgaccc gtacccagct gctctgcctg aagcacccag ttaaggttgt cgtctttcag
     3241 cgcctgttta agcagatcgc cgccgtgacc gtacaccagg tgaacgtgcg ctgcgcctaa
     3301 ttcattcgca gcatcaatga catgctgaac catcgctttc ccggcaaggg tatgcagcac
     3361 tttcggaaga tcggaataca tgcgcgtgcc tttgcctgcg gcaaggatca ctacgctcat
     3421 agcattattc aacatacgcg tccttactgt aatttgagaa cgaatttaaa ccgcttcacc
     3481 ttgaaaaaac tacatttttt tcatcgtgaa atggacagag gataaattgt tcaataacgg
     3541 attatccccg ctgacgaaag tgccattttc gaccatcgtc acgccttttg tctgcagaaa
     3601 attaaggcag taccttcatt taagcgtcag gtggatgttt ttgctcttat ttcgatcaat
     3661 gaataaacgg gaaaacaggg gtgttttata tcagcaggat ctatgtgaac gctcttcagg
     3721 acggatcacg cgtaaaaaaa agccagcctg tttccagact ggcttttgtg cttttcaagc
     3781 cggtgttaca tcgctttttt ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc
     3841 agttccgcag acgcctgagc gtaatctacg tcgccgtgag agctgctaat gtgctcttca
     3901 gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg
     3961 tcggccagaa cggtcacgtt gccaggctgc acttcaagaa tgccgccaga cagatagata
     4021 aactcttcgt gaccgtgctg tttcacgatg cgaatcatac caggcttaat ggcggtgagc
     4081 agcggtgcgt ggccagggta gatccccagt tcaccttcgc tacccgttac ctggattttc
     4141 tcgaccagac cagagaacat ttgttgctct gcactgacga cgtccaggtg gtaagtcatt
     4201 gccatatcac cctccgatta aggcgttaaa gttttttggc tttttccaca gcttcttcga
     4261 tggaaccgac catgtagaac gcctgctccg gcaggtgatc gtattcgcct tccatgatgc
     4321 ctttaaagcc acggatggtg tctttcaggg agacgtattt acccggagaa ccggtgaata
     4381 cttctgccac gaagaacggc tgggacagga agcgctggat cttacgagca cgcgctacca
     4441 ccagtttgtc ttcttcagac agttcatcca tacccaggat ggcgatgatg tctttcagtt
     4501 cctgataacg ttgcaggatg gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa
     4561 ccaccagcgg gtccagctga cggctggtgg agtccagcgg gtcaacggcc gggtagatac
     4621 ccagagacgc gatctgacgg ctcagtacca cggttgcgtc aaggtgcgca aaggtggttg
     4681 ccggagacgg gtcagtcaag tcatccgcag gtacgtatac cgcctgtacg gaggtgatag
     4741 aaccagtttt ggtggaggtg atacgttcct gcagaacgcc catctcttcc gccagggtcg
     4801 gctgataacc taccgctgaa ggcatacggc ccagcagtgc ggatacttcc gtaccggcca
     4861 gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt
     4921 tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg gtttcccggc ggctcgttca
     4981 tctggccata caccagggat actttgtcga taacgttgga gtcggtcatt tcgtggtaga
     5041 agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct
     5101 cgatcgcgat gttacgaatg agctccatca tgtttacggt tttacctaca cccgcaccac
     5161 cgaacagacc aactttaccg cccttagcga acggacacat caggtcgata actttgatac
     5221 cggtttccag cagttcctga gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa
     5281 tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc gaccggttca cccagtacgt
     5341 tcatgatacg gcccagagtc gctttaccta ccgggacttc gatcgggtgt tcgaggtctt
     5401 ttacatccag accgcgacgc agaccgtcgg aggaacccat tgcgatggta cgcacgatac
     5461 cgccgccgag ctgctgctga acttccagca ccagacgctc attaccattt tgcacctcaa
     5521 gagcatcgta cacgcgcggt acggcatcct gagggaattc gacgtcaact acggcgccga
     5581 ttacctggac aatctttcca gtagccatct taaatcctct acgaaataac ctgtttaaac
     5641 cgcggcggcc cccgagacga tctcggtgag ttcctgagta atgctggcct gacgagcttt
     5701 gttgtatacc aactgcagct ctttaatcag gctgccgcca ttgtcggtcg cggctttcat
     5761 cgccaccata cgggcggcct gctcgctggc caggttttca accacgccct gataaacctg
     5821 agattcgaca taacgacgca gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag
     5881 gtaatcccag gatttatgtt tcagatcatc atcatctgat gccggtaacg gcagcagctg
     5941 gctgatggtc ggaacctgag acatggtgtt aataaatttg ttgctgacaa tgtaaagctt
     6001 gtccagacgg ccttcgtcgt aggcctgcaa catcactttt accggaccga tcagttcgga
     6061 cagggaaggg ttatccccca tgccggtgac ctgggcaaca acattgccgc ccacggagtt
     6121 gaagaacgac acgcctttcg agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt
     6181 ccaggtcttc atttccgcca gcagtttttt gaacaggtta atgttcaaac caccgcacaa
     6241 accacggtcg gtcgacacca ccaggtagcc cacgcgttta acgtcgcggt cttccaggta
     6301 agggtgctta tattccagat taccgtgtgc aaggtgacca atcactttgc gcatggtttc
     6361 tgcataagga cggctggccg ccatgcgatc ctgcgattta cgcattttgg aagcggcgac
     6421 catctccatc gctttagtga tcttttgcgt gttctggacg cttgcgatct tactacgtat
     6481 ctcttttgcg ccggccatga gcttctcctc aatgccttgc ggcctgccct aaggcaagcc
     6541 gccagacgtt accaggattg ggttgctttg aaggaatcga ggatgccttt cagcttgcct
     6601 tcgatttcgt cgttgtagcc accggtctgg ttgatctctt gcatcaacgg agcgtgatca
     6661 cggtcgacgt aagccagcag agcggcttcg aagctgccaa ttttcgacag ttcaacatcc
     6721 gccaggtaac cacgttctgc tgcgaacaga accagagact gctgcgcaac ggacatcggc
     6781 gcatactgtt tctgtttcag cagttcggtc actttctgac cgtggtcaag ctgcttacgt
     6841 gttgcatcgt caaggtcgga tgcaaactga gagaacgctg ccagttcacg atactgtgcc
     6901 agagcggtac ggataccacc ggacagtttt ttcatgatct tggtctgtgc tgcaccacca
     6961 acacgggata cggaaatacc cgggttaacc gcaggacgaa taccggcgtt gaacaggttg
     7021 gtttccagga agatctgacc atcggtaatg gagattacgt tggtcggaac gaacgcagaa
     7081 acgtcacccg cctgagtttc gataatcggc agtgcggtca gagaaccggt tttccctttc
     7141 acttcacctt tggtgaaggc ttcaacgtat tcggcgttaa cacgtgcagc acgctccagc
     7201 agacgagagt ggaggtagaa aacgtcgccc gggaatgctt cacgtcctgg cggacgacgg
     7261 agcagcaggg agatctgacg gtaagcaaca gcctgtttag acaggtcatc gtaaatgatc
     7321 agcgcatctt caccgcggtc acggaagtat tcgcccattg cgcaaccggc atacggtgcc
     7381 aggtattgca gtgcagcgga ttcagacgcg gttgctacca caacgatggt gttagccagt
     7441 gcgccgtgct cttccagttt acgtaccacg ttagaaatgg tggacgcttt ctggccgata
     7501 gcgacataga tacatttgat accggaatcg cgctggttga tgatggcatc gatagccagt
     7561 gcggttttac ctgtctgacg gtcaccgatg atcaattcac gctgaccacg accgattggg
     7621 atcatggagt caacggcttt ataaccggtc tgtaccggct gatctacgga ctgacgttcg
     7681 ataacgcccg gagcgattgc ttctacagca gagaagccgt cgtgatccag cggaccttta
     7741 ccgtcgattg gtgcacccag agtgttaacc acacggccca gcaggccacg gccaaccgga
     7801 acttccagga tacggccagt acacttaact ttcatgcctt cggcaaggtc agcgtacgga
     7861 cccataacaa ccgcacctac agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta
     7921 cccggcaggg agatcatttc accctgcata caatcggcca ggccgtgaat gcggataaca
     7981 ccgtcactta cagaaacaat agtaccttcg ttgtgagctt cactcacaac attgaactga
     8041 gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat tcagttgcat gctccagtcc
     8101 ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg
     8161 accatatcac ccgctcggat gataacgcct gccattacag acttatcgat tttgcaattc
     8221 agcttaactt tgcgtgacag acgtttttcc atcgcagcag aaattttcgc gagctgttgt
     8281 tcactcagtg cggcagcgga aatgacgtct acctcagcgg tagcctcact cacggcacga
     8341 aggtgaataa actgctccag aacatccggg agcgcgttaa gacgaccatt ttcagccatt
     8401 acccgaatca ggttctgacc gttttcgtcc agttgctcac cacaaactgc gataaacgac
     8461 tcggcgagcg tttctggcgc aagcgcgcca gagagaagct ctgccatttg ttcgtttttg
     8521 gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg
     8581 gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg taataaattc agacatcagc
     8641 ccctccctcc ttacagttca gcgacaagtt tatccacgat gtcgctgtta gcagcttcat
     8701 ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc caggatagca acttgcttac
     8761 gcagctcttc acgggcacgt ttacgctcgg cttcaatttc tgcctgcgcc tgggccacga
     8821 ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc cagaatctgc gagcggcgtt
     8881 tgttcgcctg ctcgatgatt acctgggctt ccgctttcgc ttttttcagc tggtcggtcg
     8941 cgctggcctt tgcaaggtca aggtccttat gtgctcgttc tgcggaagca aggccgtcag
     9001 caatttcttt ttgacgtttt tcgatggctg ccattaatgg cggccatacg tacttcatgc
     9061 agaacagaac gaacaggaca aacgcgatgg cctggccgag gattgttgcg ttaagattca
     9121 cagcacaatg cctctattta gttaacgttc tgatattgct ctttaaataa aagcaacgct
     9181 tactacgcga cagcgaacat cacgtacaga cccagaccta cagcgatcat cgggatagca
     9241 tccaccagac ccataacgat aaagaactga gtacgcagca gaggaatcag atcaggttga
     9301 cgcgctgcgc cttccaggaa tttacccccg aggatgccga taccgatcgc agcaccgatt
     9361 gccgccagac ccatcatcac agcggcagcc atgtacagca gatccatatt caggttttcc
     9421 atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc
     9481 agacgccatc gacagataga cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat
     9541 gatcaggatg tggaaaatgg cccacggcac attcaggatc cactgtgacc accacggcaa
     9601 cagaccagca atcagaatga aaatcagctc accggcatac atgttaccga acagtcgcaa
     9661 accaagtgaa actggtttgg acagcaggct taccccttca aggattaagt tgacaggaat
     9721 gaacgcccag tgattgaacg gctgcagcgt caactctttc gtgaagccgc cgatgccttt
     9781 cattttgatg ctgtagaaca gaatcaggat aaatacgccc aatgccatag acagcgttac
     9841 gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga cccagtacat gttcagcaat
     9901 gtacggcagc aggtcgatag gcagtaaatc catcaggttc atcaggaata cccagacgaa
     9961 gatcgtcagg gccagcggag caatcagctt gcttttgcca tggtacatgt ctttcacgct
    10021 accattaaca aagccgatca ccagctcaat cgcggtctga aacttacctg gcacaccgct
    10081 ggtcgccttt ttggctacgc tacggaataa aaccaggaac aacagaccca gcaccaccga
    10141 gaagaacatg gagtcaatat tgattgtcca gaaggtggct ggggggtttt gtggatccac
    10201 cagcgagaat gtacgcaggt ccagctgaag gttattcagg tggtgtccta tgtaatcctg
    10261 cggcgtcata ttttctgaag ccatgatgcc ttttaccctt tgttgttaat tacagccggt
    10321 gccagtatct gaaccaccag caccaaaacc cacgtaacga tcagcggcaa gaataccgcc
    10381 tttaaaaccg ccaacgccac caccagtaac accaacatcg ccagaacttt gaaagcttcg
    10441 ccaaatgcga atgtccaggc cacccggcct ttcgctggtg tatgcgcctg gtgacgccag
    10501 gcaaatatca taaacaaaac gttaggcaga aagactgcca ggcccccgct tattgcagag
    10561 acgccccaga aggggtcttt gaggctgaac agcaatccac ttgctatcac caccagtaac
    10621 tgaacgagca gaagcttccg agcaacgttt cgactcacga gcgacacaga catcacgttt
    10681 ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata aaactttctt taaggcttag
    10741 agtcaagcat caaaaagcgg tcaaattata cggtgcgccc ccgtgatttc aaacaataag
    10801 tagccaaaag gtgaataaat gtttaaatat ttttccagtg catacaattg cgacttttct
    10861 gctaaccctg ttcgatcatg aaaaactgta aataacgcgt aaacactggt gataaagcgt
    10921 gcttcagatc acatattgcg catgttcgcg cacagcatat ttatttactt ggcaaatgat
    10981 gcct
//
LOCUS       DAJSKJ010000127        10976 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000127 DAJSKJ010000000
VERSION     DAJSKJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.128"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(84..1367)
                     /locus_tag="ON345_002867"
     CDS             complement(84..1367)
                     /locus_tag="ON345_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR3072902.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1480..3414)
                     /gene="yeaG"
                     /locus_tag="ON345_002868"
     CDS             complement(1480..3414)
                     /gene="yeaG"
                     /locus_tag="ON345_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR3072903.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3850..4596
                     /gene="mipA"
                     /locus_tag="ON345_002869"
     CDS             3850..4596
                     /gene="mipA"
                     /locus_tag="ON345_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR3072904.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4686..5540
                     /gene="yeaE"
                     /locus_tag="ON345_002870"
     CDS             4686..5540
                     /gene="yeaE"
                     /locus_tag="ON345_002870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR3072905.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5591..6475)
                     /locus_tag="ON345_002871"
     CDS             complement(5591..6475)
                     /locus_tag="ON345_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR3072906.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGAALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6559..7554)
                     /gene="gapA"
                     /locus_tag="ON345_002872"
     CDS             complement(6559..7554)
                     /gene="gapA"
                     /locus_tag="ON345_002872"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3072907.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7896..8309
                     /gene="msrB"
                     /locus_tag="ON345_002873"
     CDS             7896..8309
                     /gene="msrB"
                     /locus_tag="ON345_002873"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR3072908.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8351..8623
                     /locus_tag="ON345_002874"
     CDS             8351..8623
                     /locus_tag="ON345_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR3072909.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            8993..10069
                     /locus_tag="ON345_002875"
     CDS             8993..10069
                     /locus_tag="ON345_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR3072910.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMERIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10096..>10976
                     /locus_tag="ON345_002876"
     CDS             10096..>10976
                     /locus_tag="ON345_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3072911.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     AVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMT"
BASE COUNT         2719 a         2637 c         2762 g         2858 t
ORIGIN      
        1 attgtaagct aatgtatgta ataaatgaga taatttacag taagctaaaa atcagccagg
       61 cgataatgac ctggctgata gttttagtct ttagctgttg cattttgttt atgaaacagt
      121 tcacggaaca ccggataaat atcatcctgg tcgcggatgt gctgcatcgc aaagttgtcg
      181 aaagtagatt gcagatgctc atattctcgc cacaatgtct gatgtgcacg acgggtaatt
      241 tcgatatagc tgtaataacg aacaacaggt aataattttt tcgccaggat ttcatggcaa
      301 agcggagagt catcggccca gttatcgccg tccgatgctt gtgcagcgta aatattccac
      361 tgtgccgggt tataacgctc tttcactacc tcatccatca gtttcagggc gctggaaaca
      421 atggtgccgc ctgtttcctg cgagtagaaa aactcatgtt catcgacttc tttcgcctgg
      481 gtatgatggc ggatgtatac gacttccacg ttcttatacg ttctgctgag gaacagatac
      541 agcagaatat aaaaacgctt agccatatct ttagtggatt gatccattga accggaaacg
      601 tccatcaggc aaaacatcac tgcctggctg gagggatcgg gccgcttctc gtagttcttg
      661 taacgtaaat cgaaggtgtc aataaaaggg acgcgttcaa ttttggcacg taattctgca
      721 atttctttgc gcagacgttc ctcttccagc agctgcgcag gttcactgtt gctgatgatg
      781 gccaaattct cttccagtgc atgaagttcc cgccgcttgc ctgccgtcat ggctgtgcgt
      841 cgcgccagtg agttctgcaa ggaacgcaca acgctgatat tggccggaac gccgttagcg
      901 gtataacccg cccgatgcgt tttatattcg gtcagctggc gttgttggtt ttgtttcaga
      961 ttcggtaagg ccaaatcttc aaagagcaga tcaagatact catctttcga aatctgaaag
     1021 acaaattcat cctgaccttc accatcctgg ctggcctggc cctgaccact gccggaacct
     1081 ccgccgccac cctgcggacg ttcaattcgg tcgttctgga cgaaatggtc attgcccgga
     1141 tgcacgcggt ggcgcagacc gccacgcccc tgatgaaaca tcggttcgct aatatcttcc
     1201 gtgggaatgg atacggattc gccgctgtcg acgtcagtca ccgaacgctt attaatggcc
     1261 tcggagatcg actgtttaat ttgcgcttta taacggcgta aaaaacgctg gcgattcacc
     1321 atgcttttgt ttttgccgtt cagacgccgg tcaataaacc aggtcatatg ccccccgtac
     1381 tacatttgcc aactgtatgc tacgcctgtg ttgccatttc tctggcccac ggttcgctgt
     1441 cgttgtaggc ccggtaacat gtgtaccggg ccagggttgt taagacgatt tacgtacgcg
     1501 caaataccat tcgcacagta aacgcacctg tttacgggtg tagccttttt ccatcatacg
     1561 gtcgacaaag tcgtcgtgtt tcttctgctc gtcggttgac gttttggcgt taaacgagat
     1621 aaccggcaac agctcctcgg tattggagaa cattttcttc tcgataaccg tgcgcagttt
     1681 ttcatagctg gtccagttcg gattgcgtcc gctgttattc gctctggcgc gcagtacgaa
     1741 gttaacaatc tcgttgcgga aatcttttgg attactgatc cccgccggtt tctcgatttt
     1801 ctccagctcg gcgttaagag actcgcggtc aaacagctgc ccggtatccg gatcgcgata
     1861 ctcctgatcc tgaatccaga aatccgcgta ggtaacataa cggtcgaaaa tgttttgccc
     1921 atattcggaa taggattcaa ggtaggccgt ctggatctct ttgccgataa actcggcata
     1981 tttcgggatc agataacctt tcaggaactc cagatagcgt tctgcctgct cttgtgggaa
     2041 ctgctcgcgc tcgatctgct gttccaggac gtagaacaga tggactgggt ttgccgccac
     2101 ttctacatga tcgaagttga acacgcggga gaggatctta aacgcaaaac gcgtcgacag
     2161 accgttcatc ccttcatcga caccggcgta gtcacgatat tcctgatacg acttggcttt
     2221 gggatcggtg tctttcagac tttcgccatc ataaacccgc atctttgaat aaatgctgga
     2281 gttttctggc tctttcaggc gcgaaagaat ggaaaaacgt gacagtgttt cgagcgtgcc
     2341 aggggcgcag ggggcgtgag tcaattcact gtgattaagc aatttctcgt agattttgat
     2401 ctcttcggaa atgcgcaagc aatacggcac cttcacgatg taaacacggt cgagaaaggc
     2461 ttcgttgttt ttgttattac ggaaagtgac ccattcggat tcgttcgagt gggcgaggat
     2521 aatcccgttg aacggcaggg cggagatacc ttccgtcccg ttgtagttac cttcctgagt
     2581 ggcggttaac agcggatgca gcacttttat cggtgcttta aacatctcaa cgaactccat
     2641 gatcccttgg ttggcgcggc acagcgcacc ggaatagccg taggcgtccg ggtcattctg
     2701 cgcgtagtgt tcgagtttac gaatatcgac tttcccaacc agcgcggaga tgtcctggtt
     2761 gttctcatca ccgggttccg ttttggcgat agcaatttgt tgcagaattg acggccagac
     2821 cttcactacc cggaacttag tgatatcgcc accaaattca tgcaggcgtt ttgccgccca
     2881 tggcgacatg atggtgccga gataacggcg aggaatgcca tactcttttt ccagaatctg
     2941 agcatcttcc tgcggattga aaagacagaa cggatgatcg ttgaccgggc tacgctcacc
     3001 gttggcgctc aatacataaa tcggtacgag ctgcattaat gatttcagtc gctcagcaag
     3061 cgatgattta cccccaccca caggccccag cagatacagg atttgtttct tctcttccag
     3121 cccctgagcc gcgtgtttca gataagagac aatctgttca atcgcgtctt ccatgccgta
     3181 aaactcttca aacgccggat aacgtgcaat gacccggtta gaaaagagtc gagaaagtct
     3241 gggttcctgg gctgtatcga ccatgacagg ctcaccgata gccatcaata gccgctcagc
     3301 cgcgttggca taagcactgc gatcttgccg acaagtggta agaaactcct gcagtgtgaa
     3361 ctcttcgtcc ttggcagctt catatcgctg gcgatagtga tcgaatatat tcatggtatg
     3421 ccgtcctttc gttttttagc acaggttaag agccgttcat atgaatagta gaggctcccg
     3481 gaagaggtaa actgaacgac gtctcttgct aaaacacagc aactctcatg ccattttatc
     3541 gacgatgaga acaacgtgcc cggtgataca ccttcaaaat taagcgtaga tggcaaatgg
     3601 aaaacttgcc tgcacggctt tcttaatttc aatggcatat caacaactca tccataaatt
     3661 ttgcataatt aatgtaaaga caaggctcgc caataacgct aaattcattt ggctgtaagc
     3721 gcggtgtcat ccgcgtcagg aaaattaaac agttacttta aaaaatgaaa acgtaaaaag
     3781 gttgggtttc gatgtattga cgggtaaact ttgtcgcccg ctaaacattt gtttaaggaa
     3841 tgattaattg tgaccaaact caaacttctg gcacttggag tgcttatcgc aacgtctgca
     3901 ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag gcgtaggtgt cgttgaacac
     3961 ccatataaag attacgatac cgatgtttac ccagtaccgg taatcaacta tgaaggcgat
     4021 aacttctggt tccgtggctt aggtggtggt tactacctgt ggaatgacgc aacggacaaa
     4081 ctttcaatta ccgcttattg gtcgccgctt tacttcaaag cgaaagacag tggcgatcac
     4141 caaatgcgtc acctggatga ccgtaagagc accatgatgg ctggtctgtc ttatgctcac
     4201 tttacccagt acggttacct gcgtaccacc ctggctggcg ataccctgga taacagcaac
     4261 ggcattgtct gggatatggc ctggttgtat cgttacacca acggcggcct gaccgtgacc
     4321 ccgggtatcg gtgtgcagtg gaacagcgaa aaccagaacg aatactatta tggcgtatcg
     4381 cgcaaagagt ccgctcgcag cggtctgcgt ggctataacc cgaacgacag ctggagccct
     4441 tacctggagc tgagcgccag ctacaacttc ctcggcgact ggagtgttta cggtaccgcg
     4501 cgctataccc gtctgtctga tgaagttact gacagcccga tggtggataa atcctggact
     4561 ggcctgattt ctaccgggat cacctacaaa ttctgataat gcatctttcc agggcatttt
     4621 gtgcataaaa acagggcgtc acgccctgtt ttgcattacg gtggtgcaat acctgggagg
     4681 aaacaatgca acaaaaaatg attcaattta gtggcgatgt ctcactgcca gccatagggc
     4741 agggaacatg gtatatgggc gaagatgcca gtcagcgcaa aacagaagtt gctgcactac
     4801 gcgcgggcat tgaactcggt ttaaccctca ttgataccgc cgaaatgtat gccgatggcg
     4861 gtgccgaaaa ggtggttggg gaagcattaa ccggtctgcg cgataacgtc tttctcgtct
     4921 ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat aaatgcatgc gaagccagtt
     4981 tacgccgtct caatactgat tatctcgatc tctacttatt acactggtct ggcagtttcg
     5041 cttttgaaga gactgttgca gcgatggaaa aattgatcgc ccagggaaaa atccgccgct
     5101 ggggcgtttc taaccttgat tatgctgata tgcaggaact ctggcagctg ccggggggaa
     5161 atcagtgtgc cactaatcag gtgctttacc atctcggttc acgagggatt gagtacgatc
     5221 tactcccctg gtgccagcaa cagcagatgc cggtgatggc ttacagtcca ttagcccagg
     5281 ccgggcggtt gcgcaatgga ctgttaaaaa acgcggtagt caacgaaatt gcacatgctc
     5341 acaatatcag cgcggcacaa gtattgttgg cgtgggtaat cagccatcag ggtgtgatgg
     5401 cgattccaaa agcggccacg attgcccatg tccaacagaa tgcggctgtg cttgaggtcg
     5461 aactttcgtc agcggaatta accatgctgg ataaagcgta tccggcacca aaaggaaaaa
     5521 ctgcgctgga tatggtgtga tgttgatacg tgccggatgc atcgcacccg gcacacaaca
     5581 ttaacgcaaa ttaacgtttc gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt
     5641 tggtcacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaat gttttgtagc
     5701 catcatccgg catgtcgccc atgctaactg aaagcgccgg tcccgggttc cagccgacaa
     5761 cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttca tcattaatca
     5821 cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag gtctgaatac
     5881 cgtcggtcag tacatcttct ttcgcgtcat ttactttatc aatgaagcga tcgcccagcc
     5941 cactgacgct taccttagcg atatcaccca cgttaaagta ggtatgcagg gcagaggtgg
     6001 tttcaaattc gccatgtgat tcaagatcga tttcacaggt tttacccacg cggaaatgcg
     6061 ctaacagcgt aaagtcgtgc ggccagaatt ttttcgtctc ttcgctttgc gtcaattcaa
     6121 aagtcagcgc tgcgccatca gcatcttcat gatgtgattt cagcgtccac ggcaggttgc
     6181 gggcaaaacc gtgcgcaggc aggccttgtt gtgccgccgg accaaaccac ggccagcaaa
     6241 ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca
     6301 gaacttcttc ttcacccgca ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag
     6361 aggcttttac ctggggatga tcgaccacaa tgaggtccag ttcatccagt ttacgacggg
     6421 agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta atcatgacgc
     6481 agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga
     6541 tcacagtgtc atctcaactt atttggagat gtgagcgatc aggtccagaa ctttgttgga
     6601 gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt tcagagcgat
     6661 accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg tagatactac
     6721 gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcag ctttaacagc
     6781 agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt caactacgga
     6841 tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca gttctggcag
     6901 tactttacct acagctttag cagcaccggt agaggacggg atgatgttct gggaagcgcc
     6961 gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag tagcggtagt
     7021 agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga taactttagc
     7081 cagcggagcc aggcagttgg tggtgcagga agcgttggaa acgatgtcct ggccagcata
     7141 tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg gaccagtcat
     7201 aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag tcaggaacag
     7261 accagttgct tcagcgacaa cgtcaacacc aacttcgtcc catttcaggt tagccggatc
     7321 acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt ctttcacttc
     7381 aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc
     7441 agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg
     7501 gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag tcatatattc
     7561 caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc
     7621 gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag cattacgcga
     7681 ctgactcgcc tcacttttcc tttgggcttg aaaccagatg gggtcggcgc cccgaatttt
     7741 aaagggcaat tacgataaaa atgtgatttt catcacgatt taggcgcagc catttcgtcg
     7801 agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag aatcagtcac
     7861 gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc taataaacct tcggcagaag
     7921 aactgaaaaa aaatttgtcc gagatgcagt tctacgtgac gcagaatcat gggacagagc
     7981 cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac tgtttgatct
     8041 gcgatgcccc gctgtttcat tcccaaacca agtatgattc cggctgtggt tggcccagtt
     8101 tctacgaacc ggtgagtgaa gaatccattc gttatatcaa agacttgtca catggaatgc
     8161 agcgcataga aattcgttgc ggtaactgtg atgcccatct ggggcatgtc ttccctgacg
     8221 ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc ctctttacgc tttaccgatg
     8281 gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat tccacaggag
     8341 cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg tataccagcg
     8401 tttgtcgacc gccgttgaac tggggaaatg gcccgatggc gttgcgttga ccgaggaaca
     8461 aaaagcgaac tgcctgcaac tggtgatgct gtggcaagct cgccataata ccgaagcaca
     8521 gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc agttaaaaga
     8581 agattttggt atctcagcaa agcctattgc catgtttaag taagtggttc atcccctttt
     8641 tgccgcggct atgaagggga tagccaacaa tgaccccacg actcgtctca attatctcgc
     8701 cccgacctgg ctaagtccca taccgatctc ttgcagtcgc gttcacgcct gaatgacttc
     8761 tcctgaactt aagtctcaga cctatttggc cggtaatccc tctcgaattt gctctttttg
     8821 cgtcccgcct tccatcaacc tgtaacaaac cgagccattt tctgcgtaat tgtgatctaa
     8881 gttgtaaaac gcagatcatt atctgtgttt tacagattta atgttgacct caaaaatcat
     8941 gtgggtaagt atgatggcca tctaagcaaa acaaaacaca gaggatgacg caatgaaagc
     9001 actggctcgg tttggcaagg cctttggcgg ctacaagatg attgatgtcc cacaacccat
     9061 ttgtggcccg gaagacgtag tgattgaaat taaagccgcg gcaatctgcg gcgcagacat
     9121 gaagcactac aatgtcgata gcggttctga tgagtttaac tctatccgcg gccatgagtt
     9181 cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt
     9241 cgtgtcggat aacagcggtc acgtttgcgg cgtttgtccg gcctgtgaac aaggtgattt
     9301 tctgtgttgt acagaaaaag taaaccttgg tctggataat aatacctggg gtggtggttt
     9361 ttccaaatat tgtctggttc ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga
     9421 aatccctgat ggtgttgatt atgaggacgc agccgtactt gaccctatct gtaatgccta
     9481 caaatccatc gcgcagcaat cgaaattcct tcctggtcag gatgtggtcg tcatcggcac
     9541 tggcccactc gggctgtttt ccgtacaaat ggagcggatt atgggggcgg taaatatcgt
     9601 cgtcgttggt ctgcaagaag atgtggcggt ccgcttcccg gttgcaaaag aactgggggc
     9661 gacggcagta gtaaatggtt ctaccgaaga tgtggtggcg cgctgccagc aaatttgtgg
     9721 caaagataat ctgggactgg tgattgaatg ctccggtgcc aatatcgcac tgaaacaagc
     9781 catcgaaatg ctccgcccga atggggaagt ggtacgcgtt ggaatgggct tcaaacctct
     9841 tgatttctcg attaatgaca ttaccgcctg gaataaaagc atcattgggc atatggccta
     9901 tgactccacc tcatggcgta acgctatccg gctattagcc agcggcgcta tcaaagtcaa
     9961 accgatgatc acgcatcgta tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt
    10021 cgataaaacc gcaatcaaag tgatcatgac ttacgacttt gatgaataac cactctcctg
    10081 aaggaacgtt atcgcatgga acagataaca aaaccgcatt gtggtgcacg gctggatcgc
    10141 ttacctgatt gccgctggca ttcatcaatg tttgccatcg tcgcgtttgg cttgcttgtc
    10201 tgctggagta atgccgttgg tggcttgatc ctcgcgcagc tgaaagcgtt gggctggaca
    10261 gataattcca ccacagccac attctcagca atcacgaccg ccggaatgtt tctcggtgcg
    10321 ctggtgggcg gcatcattgg cgacaaaacc ggtcgcagaa atgcgttcat cctctatgag
    10381 gccattcata ttgcctcgat ggctgtcggt gctttctcac cgaatatgga ttttcttatt
    10441 gcctgccgtt ttgtgatggg cgttgggctg ggagctttac tggttacact gtttgctggt
    10501 ttcaccgaat atatgcccgg tagaaatcgc ggaacgtggt ccagtcgggt ttcctttatt
    10561 ggcaactggt catatccgct ctgttcattg attgcgatgg gactcacgcc gctgattagt
    10621 gcagagtgga actggcgggt acaactgctt atccctgcaa tattgtcgct tatcgctacg
    10681 gcgctggcct ggcgctactt tcctgaatcc ccgcgctggc tggaatcgcg cggacggtat
    10741 caagaagccg agaaagtcat gcggagtata gaagaaggcg tcatacgcca gacgggtaaa
    10801 cctttgccgc ctgtggttat tgctgatgac ggtaaagcgc cacaagcggt gccgtattca
    10861 gccttactga caggagtatt actgaaacgc gtgatattag gctcttgtgt gctgattgcc
    10921 atgaacgttg tgcagtacac actaattaac tggttgccaa caatattcat gaccca
//
LOCUS       DAJSKJ010000128        10903 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000128 DAJSKJ010000000
VERSION     DAJSKJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.129"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            70..831
                     /gene="adiY"
                     /locus_tag="ON345_002877"
     CDS             70..831
                     /gene="adiY"
                     /locus_tag="ON345_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR3072912.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASSLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            968..2305
                     /gene="adiC"
                     /locus_tag="ON345_002878"
     CDS             968..2305
                     /gene="adiC"
                     /locus_tag="ON345_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR3072913.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2409..4052
                     /gene="eptA"
                     /locus_tag="ON345_002879"
     CDS             2409..4052
                     /gene="eptA"
                     /locus_tag="ON345_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR3072914.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4049..4717
                     /gene="basR"
                     /locus_tag="ON345_002880"
     CDS             4049..4717
                     /gene="basR"
                     /locus_tag="ON345_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR3072915.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4718..5817
                     /gene="pmrB"
                     /locus_tag="ON345_002881"
                     /pseudo
     CDS             4718..5817
                     /gene="pmrB"
                     /locus_tag="ON345_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
     gene            complement(5793..5882)
                     /gene="pmrR"
                     /locus_tag="ON345_002882"
     CDS             complement(5793..5882)
                     /gene="pmrR"
                     /locus_tag="ON345_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR3072916.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5994..7496)
                     /gene="proP"
                     /locus_tag="ON345_002883"
     CDS             complement(5994..7496)
                     /gene="proP"
                     /locus_tag="ON345_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR3072917.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7760..8626)
                     /locus_tag="ON345_002884"
     CDS             complement(7760..8626)
                     /locus_tag="ON345_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR3072918.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSMADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8623..10851)
                     /gene="crfC"
                     /locus_tag="ON345_002885"
     CDS             complement(8623..10851)
                     /gene="crfC"
                     /locus_tag="ON345_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR3072919.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAEFESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGWGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2735 a         2856 c         2672 g         2640 t
ORIGIN      
        1 ataaccattt cccggtatcg ctttgcttta gcgagaactg gtcttttatt cgcactcagg
       61 agtacatgta tgaggatttg cagcgaccaa ccttgtattg ttttattgac tgaaaaagat
      121 gtctggataa gggtgaatgg gaaagaacct attagcctta aagctaacca tatggcgtta
      181 ttaaattgtg aaaataatat tatcgacgtc tcctctctta acaacacttt ggttgctcat
      241 attagtcacg acatcatcaa agattacctc cggtttctga ataaagatct ctcgcaaata
      301 ccagtatggc aacgtagcgc tacgcccata ctcaccctgc catgcctgac gccagacgtc
      361 tttcgcgttg ccgcgcaaca cagcatgatg cccgcagaaa ctgagtcaga aaaggaacga
      421 acacgcgcat tattattcac tgtgctatcc cgttttctcg acagtaaaaa attcctttca
      481 ttaatgatgt atatgttacg taattgtgta agtgacagcg tttatcaaat tattgaaagc
      541 gatattcaca aagactggaa tcttagtatg gtagccagtt ctttatgtct tagcccaagt
      601 ctgttaaaga aaaagttgaa aagcgaaaac accagttata gccaaataat caccacctgc
      661 cgcatgcgtt atgccgtaaa tgaattaatg atggacggta aaaatatctc tcaggtatca
      721 cagtcctgcg gctacaacag tacgtcgtac tttatttctg tctttaaaga cttctacggt
      781 atgacgccgc tgcattatgt tagtcagcac agagaacgca ctgtcgcctg atttttaacc
      841 ttaacgaaga gctatattaa taacggcatc agcgataacc cggtcgataa taattcaact
      901 atcgaatgca ggcgtatgat atgacgtaat ttattgtcac gaagctcgcc ttcgcaggag
      961 tttaattatg tcttcggatg ctgatgctca caaagtgggc ttaatccccg tcaccctgat
     1021 ggtgtcgggg aatattatgg ggtcaggtgt ttttctgtta cctgcaaacc tggcctctac
     1081 tggcgggatt gccatttatg gatggttggt gacgattatc ggtgcgctgg ggctctcgat
     1141 ggtatacgcc aaaatgtcgt tcctcgatcc aagtcctggt ggttcttacg cgtacgcccg
     1201 ccgctgcttt ggcccgtttc tcggttatca aaccaacgtc ctctactggc tggcctgctg
     1261 gatcggcaat atcgccatgg tggtcattgg cgtaggatat ttaagttact tcttcccgat
     1321 tctgaaagat ccattggtat taaccatcac ctgcgtcgtg gtgctgtgga tcttcgtcct
     1381 gctgaacatt gtcggtccga aaatgatcac ccgtgtgcag gccgttgcca ccgtgctggc
     1441 gctgatcccc atcgtcggga ttgccgtatt tggctggttc tggttccgtg gtgaaaccta
     1501 tatggcggca tggaacgtca gcggcctggg caccttcggt gcaattcaaa gtacccttaa
     1561 cgttacgctg tggtcgttca tcggtgtgga aagtgcctcc gttgccgcag gtgtggtgaa
     1621 aaacccgaaa cgcaacgtcc ctatcgccac cattggtggg gtattgattg ccgccgtttg
     1681 ctatgtgctc tccaccaccg cgattatggg gatgatccct aatgccgcac tgcgcgtttc
     1741 tgcctcgcca ttcggtgatg ccgcacggat ggcgttgggt gacaccgccg gggccattgt
     1801 ttccttctgc gcagctgcgg gttgcttagg ttcactgggc ggctggacat tgctggcggg
     1861 tcaaacggcg aaagccgctg ccgatgacgg actgttccca ccaatttttg cccgtgtaaa
     1921 taaagcgggc acgccagtgg cggggctgat tatcgtcggt attttgatga ccatcttcca
     1981 gctcagcagc atttcaccaa acgcgaccaa agagttcggt ctggtttctt ccgtctcggt
     2041 catctttaca ctggtgccat atctttacac ctgtgcggcg ttactgctgc tcggccacgg
     2101 tcactttggt aaagcacgcc cggcatatct ggctgttact accattgcct tcctctactg
     2161 catttgggcc gtggtggggt ccggggcgaa agaggttatg tggtcatttg tcaccctgat
     2221 ggtcatcacc gccatgtatg cgctgaatta caaccggcta cataaaaacc cgtatccctt
     2281 agatgcacca ataagcaaag attaattccc tttaatccag caaacataaa agccaacctt
     2341 aagaacttaa ggttggctta attttgcttt gcgagcatat gcgcactttg ttcgatggaa
     2401 acaccgtgat gttgaagcgc ctactaaaaa gaccctcttt gaatttactc gcctggctat
     2461 tgttggccgc tttttatatc tctatctgcc tgaatattgc cttttttaaa caggtgttgc
     2521 aggcgctgcc gctggattcg ctgcataacg tactggtttt cttgtcgatg ccggtcgtcg
     2581 ctttcagcgt gattaatatt gtcctgacac taagctcttt cttatggctt aatcgaccac
     2641 tggcctgcct gtttattctg gttggcgcgg ctgcacaata tttcataatg acttacggca
     2701 tcgtcatcga ccgctcgatg attgccaata ttattgatac cactccggca gaaagttatg
     2761 cgctgatgac accgcaaatg ttattaacgc tgggattcag cggcgtgctt gctgcgctga
     2821 ttgcctgctg gataaaaatc aaacctgcca cctcgcgtct gcgcagtgtt cttttccgtg
     2881 gagccaatat tctggtttct gtactactga ttttgctggt cgccgcactg ttttataaag
     2941 actacgcctc gttgttccgc aataacaaag agctggtgaa atccttaagc ccctctaaca
     3001 gcattgttgc cagctggtca tggtactccc atcagcgact ggcaaatctg ccgctggtgc
     3061 gaattggtga agacgcgcac cgcaacccgt taatgcagaa cgaaaaacgt aaaaatttga
     3121 ccatcctgat tgtcggcgaa acctcgcggg cggagaactt ctccctcaac ggctacccgc
     3181 gtgaaactaa cccgcggctg gcgaaagata acgtggtcta tttccctaat accgcatctt
     3241 gcggcacggc aacggcagtt tcagtaccgt gcatgttctc ggatatgccg cgtgagcatt
     3301 acaaagaaga gctggcacag caccaggaag gcgtgctgga tatcattcag cgagcgggca
     3361 tcaacgtgct gtggaatgac aacgatggcg gctgtaaagg tgcctgcgac cgcgtgcctc
     3421 accagaacgt caccgcgctg aatctgcctg gtcagtgcat caacggcgaa tgctatgacg
     3481 aagtactgtt ccacgggctg gaagagtaca tcaataacct gcagggtgat ggcgtgattg
     3541 tcttacacac catcggcagc cacggtccga cctattacaa ccgctatccg ccgcagttca
     3601 ggaaatttac cccaacctgc gacaccaatg agatccagac ctgtagcaaa gagcaactgg
     3661 tgaacactta cgacaacacg ctggtttacg tcgactatat tgttgataaa gcgattaatc
     3721 tgctgaaaga acatcaggat aaatttacca ccagcctggt ttatctttct gaccacggtg
     3781 aatcgttagg tgaaaatggc atctatctgc acggtctgcc ttatgccatc gccccggata
     3841 gccaaaaaca ggtgccaatg ctgctgtggc tgtcggagga ttatcaaaaa cggtatcagg
     3901 ttgaccagaa ctgcctgcaa aaacaggcgc aaacgcaaca ctattcacaa gacaatttat
     3961 tctccacgct attgggatta actggcgttg agacgaagta ttaccaggct gcggatgata
     4021 ttctgcaaac ttgcaggaga gtgagtgaat gaaaattctg attgttgaag acgatacgct
     4081 gttattgcag ggattgattc tggcggcgca aaccgaaggc tacgcgtgcg atggcgtgac
     4141 aaccgcgcgg atggcggaac aaagccttga ggccggtcat tacagcctgg tggtactgga
     4201 tttagggtta cccgacgaag atggactgca ttttctcgcc cgtatccggc agaaaaaata
     4261 caccctgcca gtactgatcc tcaccgctcg cgatacgctg accgacaaaa tcgccgggct
     4321 ggatgttggt gccgatgact atctggtgaa gccttttgcg ctggaagagt tacacgcccg
     4381 tatccgcgcc ctgctacgac gccataataa tcagggcgaa agtgagctga ttgttggcaa
     4441 tctgacgctg aatatgggtc gccgtcaggt atggatgggc ggtgaagagt tgattctgac
     4501 gcccaaagaa tatgctctgc tgtcacggtt aatgctcaaa gcaggcagtc cggtgcatcg
     4561 ggaaattctc tacaacgaca tctataactg ggacaatgaa ccctcgacca acaccctgga
     4621 agtgcatatc cacaatctgc gcgacaaagt gggcaaagcc cgtatccgca ccgtgcgcgg
     4681 ctttggctat atgctggtcg cgaatgagga aaactaattg aatctgatgc attttctgcg
     4741 ccgaccaata tcgctgcgcc aacggctgat attgaccatc ggggccattt tgttggtgtt
     4801 tgagctgatc agcgtcttct ggctatggca tgaaagtacc gagcagattc agctgtttga
     4861 gcaggcgcta cgcgacaatc gcaacaacga tcgccacatc atgcgtgaga tccgcgaggc
     4921 ggtcgccagc ctgattgtcc ccggcgtctt tatggtcagc ctgacgctat ttatctgcta
     4981 tcaggcggta cgccgcatca cccgcccgct ggcggagctg caaaaagagc tggaagcgcg
     5041 cactgccgac aacttaacgc ccattgccat tcacagcgtc accctcgaaa tcgaagcggt
     5101 ggtttcggcg ttgaacgatc tggtcagtcg cctgaccagc acgctggata acgaaaggtt
     5161 gtttaccgct gacgtcgcgc acgaactgcg aacgccactg gcgggggtgc gtttgcatct
     5221 ggaactgctg gcgaaaacgc atcacattga tgtagcaccg ttagtggcac ggcttgatca
     5281 gatgatggag agcgtctccc agctgctgca actggcgcgt gccggacagt cattttcttc
     5341 cggtaattat caagatgtaa aactgctgga agatgtcatt ctcccctcgt atgacgaact
     5401 cagtaccatg ctcgaccagc gacagcaaac cctgctattg ccagagagcg ccgccgacat
     5461 caccgttcag ggtgatgcaa ccctgctgcg gatgttattg cgaaacctgg tagaaaacgc
     5521 ccatcgttac agcccgcaag gcagcaacat tatgattaag ctgcaagaag atggcggagc
     5581 ggtcatggca gttgaagatg aaggaccagg tattgatgag agtaaatgcg ggagttgagt
     5641 aaagcgtttg tacgtatgga cagccgttat ggcgggattg gtctggggtt aagtattgtc
     5701 agccgcatta cacagttgca tcacgggcag tttttcctgc aaaaccggca agagacttcc
     5761 ggcacgcggg cctgggtacg gctgaagaaa gatcagaacg tggcaaacca gatataaaga
     5821 aagctgctga ccaccagcac gctgaacacg gtagttaaac tttcataaac acggtttttc
     5881 attgcactct cctccgttaa cctggaggag agtatgcgcg tcagagatta agtgaacctt
     5941 aagagttcaa ttggcctgat aagacagcgt cacatcaggc catccgtttc agcttattca
     6001 tcaattcgcg gatgttgctg caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg
     6061 tggtcaatat tatcgatttt ctgctcgata ttatcgtaat gctcgacgag aatttccttc
     6121 gcttcctgta tatctgacgc cgccggtgtc gcacctttca acggacgatt tgccgtctct
     6181 ttcatggtta cgccggtgat taaaccaatc accgccacta ccatcaggta ataggcaggc
     6241 atcatcagat tctgcgagct ttcgaccagc caggccgcca gtgttggcgt cagaccggca
     6301 accagcaccg aaatattaaa tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac
     6361 atcgctggca aggtagaagc cataacgccc gtaaagcagt taaggatcac cgccagcatc
     6421 agtaacccgg caaaaatcag gccgatgacg ttactgttaa tcagaataaa cgccgggatc
     6481 gccaacacaa acagggcaac actaccaagt agcacaaacg gacgacggcc aaaacggtca
     6541 ctcagcaagc ccatcaccgg ctggacaaac agcataccga tcataatggc gataataatc
     6601 agcaccccgt ggtcttcgga gtaatgcagg ttatgcgaca aataactcgg catataggtc
     6661 agcaacatgt agtaagtcac gttggtggca attaccagac caatacatgt caacaggctg
     6721 cgccagtatt tagtggcaat ctctttaaac gagactttcg ggccatcctg caaaccttcg
     6781 cggtcgccct gttccagttt atcaacatgc tgctggaacg ccggagtttc ttccagcgca
     6841 tggcgcaggt aaagcccgat aatccctaac ggcagagcaa taaagaacgg aatacgccag
     6901 ccccagtcga ggaagttcgc ttcgccgaca atggtcgaaa ttaacaccac cacgccagca
     6961 cccagcacaa acccggcaat agaaccgaag tccagccagc tgcccataaa gccacgttta
     7021 cggtcagggg agtattccgc aacaaatatc gacgccccgg tatattcacc gccgaccgag
     7081 aaaccttgtg ccatcttaca gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg
     7141 taggacggta ttaagccaat acagaacgta ctgatcgaca taatcacaat agtgatagcg
     7201 aggatcttct ggcgaccata tttatcgccc aacataccaa agaagagccc gccaagcggt
     7261 cgaatcagaa agggaacgga gaaagtggca agtgcagcaa ccatctgcac gctggggtca
     7321 gcccccggga aaaaaacttt acctaatgcg taagcaacaa aaccataaac accaaaatcg
     7381 aaccattcca ttgcattacc cagtgatgct gcggtaatgg ctttacgcag tttaccgtca
     7441 tcaataatgg tgacatcacg aagggtaatc ggttttactt ttttcctttt cagcatagct
     7501 ttcctcgcag agttggccca tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa
     7561 aggggagcgg gaattgcagg atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc
     7621 ctccggttaa ggaatgtaca atcaaaccag ttgacgcctg aattgggcag ttaatgacct
     7681 ttttacccta ccagtgttta aatttgtgat caacttcaca catggatttc gtttaggact
     7741 cattgatgta actgattatt tacgccaaat ttacagagaa gatttcctcg accattgccc
     7801 ccaccaaacg ctgcgctgaa cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt
     7861 gggtcactga ccagcaaacg ggctgtttat ctgccatgga ccactttccc agccaactta
     7921 aaaaatcagc atgctcccct cccgaagagg caaaccactg atggtaatag tgacgcgtgg
     7981 tcggtgccgc attgatactc tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca
     8041 gcaacaacac caccgcttgc gggcttccca gacggcaggc atcgccaaat gcaccccagc
     8101 gcaactcctc cagaacgata aaacagcgcc caggcaacga ccaggcgtta tacgccccag
     8161 cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt
     8221 gcgttaacgc cttttccttc cagcgacaac gggctgaaaa ccactcatac aaggcggcaa
     8281 tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt tgcagcagcg
     8341 ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg atccagcatc gacgccagtt
     8401 tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa catgccgccg ttcgtccatg
     8461 tctgaagctg cacacgcgaa agaagttcct gcatcagtcg ttcacgaaac tgctgctgat
     8521 gggcttgtgg aaggcgggca tcatcgccct gcgccagatc cactaaaaag cggggataaa
     8581 ccgactccag cacgcgaccg gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt
     8641 gaaaagtgtt tgaatatcat cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt
     8701 catcacagtc tgctgcaatt gctgctgcat cagcctgacc accgattcat tttgttgacg
     8761 cagagccagg ctttcgcgta aacgcgtctg taattccgtc aaacatgaag cgaactcagc
     8821 gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca gaactttagc
     8881 catttgttgg caataaagat cgacttctgc gcttaatgct cgttgcaaca cactgtaatc
     8941 aaccgtttct gtcggggatt tctcatttcc ccatccccag tcgggctgat tcaaccagcg
     9001 cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc tcgtccgttg cctcctggcg
     9061 cgaaatgact tcactgaaca gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac
     9121 aggaaaatga aacccaggac gaaaccctga ctcgctcaat tccattttga cttgttgctc
     9181 aatgggccga ataacatcgg ttaacacccg gcaaagggtg gattcaaact cggcaaaacg
     9241 cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct cacagcaggt
     9301 acgaatctcg cttaacggct ccgaatcatc ctgaaacaag gcggctaact gcgcattcag
     9361 cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc agggccagct cgatttcatg
     9421 tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac tttcttcgac
     9481 ctgatggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg cacgaaaatc
     9541 caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcagcag atcgcagagc
     9601 atacaatgag gcgttagcgt aagcagcatg aagcaacgcc tgaattggct gggcgaacag
     9661 cgaatcttcc cacaactgat cggcagcatg acgaatatgt tcgaggtccg ccagatcggc
     9721 atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc gttgttgctc
     9781 tggcgctggt aacttaccgt tgttggctaa ctcatagcgc gcccgattcg ccaggtagcc
     9841 ccacatcgac gacaccggaa atatctgctg tggcgtaata cagcctttca tcagcgtccc
     9901 ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt
     9961 attgaccagc acatacagcg gcaccgattg ccccaccgcc aaaatcgcct cacggacctc
    10021 ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg ccgaggcgcg
    10081 tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat ttggcccggg
    10141 ggtatccagt aacgtcaact gaccgggata actttccagc cccgccagat ggacaaactc
    10201 cacttcaatc acgggaatat gctcaatggc agcgtaagca gaaaaaggaa aatcgacgtc
    10261 cagcgccttc gccagtcgca ctaaatcatt caaacttttc agacaatgaa aaataggctg
    10321 ggcacccaga taatattttt cgaaagcgac gccattttcg atccgctgca taagcgcacg
    10381 catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat cacgcaggcg
    10441 ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca tgtgaaaaat gcagtactgg
    10501 ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc gcagtcattg ggcgattacg
    10561 attaggcaga acctccgtac caacaatggc attaatggtg gttgatttcc ctgctttcat
    10621 agtaccgaca atcgccagca ccatttccag tcgggaaatt ttacgcaact cattattcag
    10681 catcgcgtaa cgttcggcga tattaggctg actccagggt aaagccagtt gtggcgcatc
    10741 gtctccgggt acagagagag gcattttttc cagtaactgc aactgttggc gagaaagctg
    10801 taacaggcgt tcagcctcct gacttaactc atacagggtc tgtgtgtaca tagaaaattc
    10861 ttccttaaag caaattttgt tacttttatt tagccagatt gtt
//
LOCUS       DAJSKJ010000129        10838 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000129 DAJSKJ010000000
VERSION     DAJSKJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.130"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            267..671
                     /gene="sdhC"
                     /locus_tag="ON345_002886"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             267..671
                     /gene="sdhC"
                     /locus_tag="ON345_002886"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR3072920.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPGGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            665..1012
                     /gene="sdhD"
                     /locus_tag="ON345_002887"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             665..1012
                     /gene="sdhD"
                     /locus_tag="ON345_002887"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR3072921.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1012..2778
                     /gene="sdhA"
                     /locus_tag="ON345_002888"
     CDS             1012..2778
                     /gene="sdhA"
                     /locus_tag="ON345_002888"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR3072922.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVTLGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEICEGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2794..3510
                     /gene="sdhB"
                     /locus_tag="ON345_002889"
     CDS             2794..3510
                     /gene="sdhB"
                     /locus_tag="ON345_002889"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR3072923.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3811..6612
                     /gene="sucA"
                     /locus_tag="ON345_002890"
     CDS             3811..6612
                     /gene="sucA"
                     /locus_tag="ON345_002890"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR3072924.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6627..7844
                     /gene="odhB"
                     /locus_tag="ON345_002891"
     CDS             6627..7844
                     /gene="odhB"
                     /locus_tag="ON345_002891"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR3072925.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7938..9104
                     /gene="sucC"
                     /locus_tag="ON345_002892"
     CDS             7938..9104
                     /gene="sucC"
                     /locus_tag="ON345_002892"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR3072926.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9104..9973
                     /gene="sucD"
                     /locus_tag="ON345_002893"
     CDS             9104..9973
                     /gene="sucD"
                     /locus_tag="ON345_002893"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR3072927.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIISRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10077..10799)
                     /gene="mngR"
                     /locus_tag="ON345_002894"
     CDS             complement(10077..10799)
                     /gene="mngR"
                     /locus_tag="ON345_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR3072928.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2550 a         2809 c         3001 g         2478 t
ORIGIN      
        1 cccgacaaac tatatgtagg ttaattgtaa tgattttgtg aacagcctat actgccgcca
       61 ggtctccgga acaccctgca atcccgagcc acccagcgtt gtaacgtgtc gttttcgcat
      121 ctggaagcag tgttttgcat gacgcgcagt tatagaaagg acgctgtctg acccgcaagc
      181 agaccggagg aaggaaatcc cgacgtctcc aggtaacaga aagttaacct ctgtgcccgt
      241 agtccccagg gaataataag aacagcatgt gggcgttatt catgataaga aatgtgaaaa
      301 aacaaagacc tgttaatctg gacctacaga ccatccggtt ccccatcacg gcgatagcgt
      361 ccattctcca tcgcgtttcc ggtgtgatca cctttgttgc agtgggcatc ctgctgtggc
      421 ttctgggtac cagcctctct tcccctggag gtttcgagca agcttccgcg attatgggca
      481 gcttcttcgt caaatttatc atgtggggca tccttaccgc tctggcgtat cacgtcgtcg
      541 taggtattcg ccacatgatg atggattttg gctatctgga agaaacattc gaagcgggta
      601 aacgctccgc caaaatctcc tttgttatta ctgtcgtgct ttcacttctc gcaggagtcc
      661 tcgtatggta agcaacgcct ccgcattagg acgcaatggc gtacatgatt tcatcctcgt
      721 tcgcgctacc gctatcgtcc tgacgctcta catcatttat atggtcggtt ttttcgccac
      781 cagtggcgaa ctgacatatg aagtctggat cggtttcttc gcctctgcgt tcaccaaagt
      841 gttcaccctg ctggcgctgt tttctatctt gattcatgcc tggatcggca tgtggcaggt
      901 gttgaccgac tacgttaaac cgctggcatt gcgcctgatg ctgcaattgg tgattgtcgt
      961 tgcactggtg gtttacgtga tttatggatt cgttgtggtg tggggtgtgt gatgaaattg
     1021 ccagtcagag aatttgatgc agttgtgatt ggtgccggtg gcgcaggtat gcgcgcggcg
     1081 ctgcaaattt cccagagcgg ccagacctgt gcgctgctct ctaaagtctt cccgacccgt
     1141 tcccataccg tttctgcgca aggcggcatt accgttacgc tgggtaatac ccatgaagat
     1201 aattgggaat ggcatatgta cgacaccgta aaagggtcgg actatatcgg tgaccaggac
     1261 gcgattgaat atatgtgtaa aaccgggccg gaagcgattc tggaactcga acacatgggc
     1321 ctgccgttct cgcgtcttga tgatggtcgt atctatcaac gtccgtttgg cggtcagtcg
     1381 aaaaacttcg gcggcgagca ggcggcacgc actgcggcgg cggctgaccg taccggtcac
     1441 gcactgttgc acacgcttta tcagcagaac ctgaaaaacc acaccaccat tttctccgag
     1501 tggtatgcgc tggatctggt gaaaaaccag gatggcgcgg tggtgggttg taccgcactg
     1561 tgcatcgaaa ccggtgaagt ggtttatttc aaagcccgcg ctaccgtgct ggcgactggc
     1621 ggggcagggc gtatttatca gtccaccact aacgcccaca ttaacactgg cgacggtgtc
     1681 ggcatggcta tccgcgcagg cgtaccggtg caggatatgg aaatgtggca attccacccg
     1741 accggcattg ccggtgcggg cgtactggtc accgaaggtt gccgtggtga aggcggttat
     1801 ctgctgaaca aacatggcga acgctttatg gagcgttatg cgccgaacgc caaagacctg
     1861 gcgggccgtg atgtggtggc gcgttccatc atgattgaaa tctgtgaagg tcgcggctgt
     1921 gatggtccgt gggggccaca cgcgaaactg aaactcgatc acctgggtaa agaagttctc
     1981 gaatcccgtc tgccgggtat cctggagctt tcccgtacct tcgctcacgt cgatccggtg
     2041 aaagagccga ttccggttat cccaacctgt cactacatga tgggcggtat tccgaccaaa
     2101 gtgaccggtc aggcgctgac ggtgaacgag aaaggcgaag atgtggttgt tccggggctg
     2161 tttgccgttg gtgaaatcgc ttgtgtatcg gtacacggcg ctaaccgtct gggcggcaac
     2221 tcgctgctgg acctggtggt ctttggtcgt gcggcaggtc tgcatctgca agagtctatc
     2281 gccgaacagg gcgcattgcg cgatgccagc gagtctgatg ttgaagcatc tctggatcgt
     2341 ctgaaccgct ggaacaataa tcgtaacggt gaagatccgg tggcgattcg taaagcgctg
     2401 caagaatgta tgcagcataa cttctcggtc ttccgtgaag gtgatgcgat ggcgaaaggg
     2461 cttgagcagt tgaaagttat ccgcgagcgt ctgaaaaatg cccgtctgga tgacacttcc
     2521 agcgagttca acacccagcg cgttgagtgc ctggaactgg ataacctgat ggaaacggcg
     2581 tatgcaacgg ctgtttctgc caacttccgt accgaaagcc gtggcgcaca tagccgcttc
     2641 gacttcccgg atcgtgatga tgaaaactgg ctgtgccact ccctgtatct gccagagtcg
     2701 gaatccatga cgcgccgaag cgtcaacatg gaaccgaaac tgcgcccggc attcccgccg
     2761 aagattcgta cttactaatg cggagacagg aaaatgagac tcgagttttc aatttatcgc
     2821 tataacccgg atgttgatga tgctccgcgt atgcaggatt acaccctgga agcggaagaa
     2881 ggtcgcgaca tgatgctgct ggatgcgctt atccagctga aagagaaaga tcccagcctg
     2941 tcgttccgcc gctcctgccg tgaaggtgtg tgcggttccg acggtctgaa catgaacggc
     3001 aagaatggtc tggcctgtat taccccgatt tcggcactca accagccggg caagaagatt
     3061 gtgattcgcc cgctgccagg tttaccggtg atccgcgatt tggtggtaga catgggacaa
     3121 ttctatgcgc aatatgagaa aattaagcct tacctgttga ataatggaca aaatccgcca
     3181 gctcgcgagc atttacagat gccagagcag cgcgaaaaac tcgacgggct gtatgaatgt
     3241 attctctgcg catgttgttc aacctcttgt ccgtctttct ggtggaatcc cgataagttt
     3301 atcggcccgg caggcttgtt agcggcatat cgtttcctga tcgatagccg tgataccgag
     3361 actgacagcc gcctcgacgg tttgagcgat gcattcagcg tattccgctg tcacagcatc
     3421 atgaactgcg tcagtgtatg tccgaagggg ctgaacccga cgcgcgccat cggccatatt
     3481 aagtcgatgt tgttgcaacg taatgcgtaa accgtaggcc tgataagacg cgcaagcgtc
     3541 gcatcaggca accagtgccg gatgcggcgt gaacgcctta tccggcctac aagtcattac
     3601 ccgtaggcct gataagcgca gcgcatcagg cgtaacaaag aaatgcagga aatctttaaa
     3661 aactgcccct gacactaaga cagtttttaa aggttccttc gcgagccact acatagacaa
     3721 gagctcgcaa gtgaaccccg gcacgcacat cactgtgcgt ggtagtatcc acggcgaagt
     3781 aagcataaaa aagatgctta agggatcacg atgcagaaca gcgctttgaa agcctggttg
     3841 gactcttctt acctctctgg cgcaaaccag agctggatag aacagctcta tgaagacttc
     3901 ttaaccgatc ctgactcggt tgacgctaac tggcgttcga cgttccagca gttacctggt
     3961 acgggagtca aaccggatca attccactct caaacgcgtg aatatttccg ccgcctggcg
     4021 aaagacgctt cacgttactc ttcaacgatc tccgaccctg acaccaatgt gaagcaggtt
     4081 aaagtcctgc agctcattaa cgcataccgc ttccgtggtc accagcatgc gaatctcgat
     4141 ccgctgggac tgtggcagca agataaagtg gccgatctgg atccgtcttt ccacgatctg
     4201 accgaagcag acttccagga gaccttcaac gtcggttcat ttgccagcgg caaagaaacc
     4261 atgaaactcg gcgagctgct ggaagccctc aagcaaacct actgtggccc gattggtgcc
     4321 gagtatatgc acattaccag caccgaagaa aaacgctgga tccaacagcg tatcgagtct
     4381 ggtcgcgcga ctttcaatag cgaagagaaa aaacgcttct taagcgaact gaccgccgct
     4441 gaaggccttg aacgttacct cggcgcaaaa ttccctggcg caaaacgctt ctcgctggaa
     4501 ggcggtgacg cgttaatccc gatgcttaaa gagatgatcc gccacgctgg caacagcggc
     4561 acccgcgaag tggttcttgg gatggcgcac cgtggtcgtc tgaacgtgct ggtgaacgtg
     4621 ctgggtaaaa aaccgcaaga cttgttcgac gagtttgccg gtaaacataa agaacacctc
     4681 ggcacgggtg acgtgaaata ccacatgggc ttctcgtctg acttccagac cgatggcggc
     4741 ctggtgcacc tggcgctggc gtttaacccg tctcaccttg agattgtaag cccggtagtt
     4801 atcggttctg ttcgtgcccg tctggacaga cttgatgagc cgagcagcaa caaagtgctg
     4861 ccaatcacca tccacggtga cgccgcagtg accgggcagg gcgtggttca ggaaactctg
     4921 aacatgtcga aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt tatcaacaac
     4981 caggttggtt tcaccacctc taatccgctg gatgctcgtt ctacaccgta ctgtactgat
     5041 atcggtaaga tggttcaggc accgattttc cacgttaacg cggatgatcc ggaagccgtt
     5101 gcctttgtga cccgtctggc gctcgatttc cgtaacacct ttaaacgtga tgtcttcatc
     5161 gacctggtgt gctaccgccg tcacggccac aacgaagccg acgagccgag cgcaacccag
     5221 ccgctgatgt atcagaaaat caaaaaacat ccgacaccgc gcaaaatcta cgctgacaag
     5281 ctggagcagg aaaaagtggc gacgctggaa gatgccaccg agatggttaa cctgtaccgc
     5341 gatgcgctgg atgctggcga ttgcgtagtg gcagagtggc gtccgatgaa catgcactct
     5401 ttcacctggt cgccgtacct caaccacgaa tgggacgaag agtacccgaa caaagttgag
     5461 atgaagcgcc tgcaggagct ggcgaaacgc atcagcacgg tgccggaagc agttgaaatg
     5521 cagtctcgcg ttgccaagat ttatggcgat cgccaggcga tggctgccgg tgagaaactg
     5581 ttcgactggg gcggtgcgga aaacctcgct tacgccacgc tggttgacga aggcattccg
     5641 gttcgcctgt cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca cgcggtgatc
     5701 cacaaccagt ctaacggttc cacttacacg ccgttgcaac acatccataa cgggcagggc
     5761 gcgttccgcg tctgggactc cgtactgtct gaagaagcag tgctggcgtt tgaatacggt
     5821 tatgccaccg cagaaccacg cactctgacc atctgggaag cgcagttcgg tgacttcgcc
     5881 aacggtgcgc aggtggttat cgaccagttc atctcttctg gcgaacagaa atggggccgg
     5941 atgtgtggtc tggtgatgtt gctgccgcac ggttacgaag ggcaggggcc ggagcactcc
     6001 tccgcgcgtc tggaacgtta tctgcaactt tgtgctgagc aaaacatgca ggtttgcgtt
     6061 ccgtctaccc cggcacaggt ttaccacatg ctgcgtcgtc aggcgctgcg cgggatgcgt
     6121 cgtccgctgg tagtgatgtc gccgaaatcc ctgctgcgtc atccgctggc ggtttccagc
     6181 ctcgaagaac tggcgaacgg caccttcctg ccagccatcg gtgaaatcga cgagcttgat
     6241 ccgaagggcg tgaagcgcgt agtgatgtgt tctggtaagg tttattacga cctgctggaa
     6301 cagcgtcgta agaacaatca acacgatgtc gccattgtgc gtatcgagca actctacccg
     6361 ttcccgcata aagcgatgca ggaagtgttg cagcagtttg ctcacgtcaa ggattttgtc
     6421 tggtgccagg aagagccgct caaccagggc gcatggtact gcagccagca tcatttccgt
     6481 gaagtgattc cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc cgcctctccg
     6541 gcggtagggt atatgtccgt tcaccagaaa cagcaacaag atctggttaa tgacgcgctg
     6601 aacgtcgaat aaataaagga tacacaatga gtagcgtaga tattctggtc cctgacctgc
     6661 ctgaatccgt agccgatgcc accgtcgcaa cctggcataa aaaacccggc gacgcagtcg
     6721 tacgtgatga agtgctggta gaaatcgaaa ctgacaaagt ggtactggaa gtaccggcat
     6781 cagcagacgg cattctggat gcggttctgg aagatgaagg tacaacggta acgtctcgtc
     6841 agatccttgg tcgcctgcgt gaaggcaaca gcgccggtaa agaaaccagc gccaaatctg
     6901 aagagaaagc gtccactccg gcgcaacgcc agcaggcgtc tctggaagag caaaacaacg
     6961 atgcgttaag cccggcgatc cgtcgcctgc tggctgaaca caatctcgac gccagcgcca
     7021 ttaaaggcac cggtgtgggt ggtcgtctga ctcgtgaaga tgtggaaaaa catctggcga
     7081 aagccccggc gaaagagtct gctccggcag cggctgctcc ggcggcgcaa ccggctctgg
     7141 ctgcacgtag tgaaaaacgt gtcccgatga ctcgcctgcg taagcgtgtg gcagagcgtc
     7201 tgctggaagc gaaaaactcc accgccatgc tgaccacgtt caacgaagtc aacatgaagc
     7261 cgattatgga tctgcgtaag cagtacggtg aagcgtttga aaaacgccac ggcatccgtc
     7321 tgggctttat gtccttctac gtgaaagcgg tggttgaagc cctgaaacgt tacccggaag
     7381 tgaacgcgtc tatcgacggc gatgacgtgg tttaccacaa ctatttcgac gtcagcatgg
     7441 cggtttctac gccgcgcggc ctggtgacac cggtactgcg tgatgtcgat accctcggca
     7501 tggcagacat cgagaagaaa atcaaagagc tggcagtcaa aggccgtgac ggcaagctga
     7561 ccgttgaaga tctgaccggt ggtaacttca ccatcaccaa cggtggtgtg ttcggttccc
     7621 tgatgtctac gccgatcatc aacccgccgc agagcgcaat tctgggtatg cacgctatca
     7681 aagatcgtcc gatggcggtg aatggtcagg ttgagatcct gccgatgatg tacctggcgc
     7741 tgtcctacga tcaccgtctg atcgatggtc gcgaatccgt gggcttcctg gtaacgatca
     7801 aagagttgct ggaagatccg acgcgtctgc tgctggacgt gtagtagttt aagcttcacc
     7861 tgcactgtag accgacgtgg atagggtgtc ggtctacggt ttaaaagata acgattactg
     7921 aaggatggac agaacacatg aacttacatg aatatcaggc aaaacaactt tttgcccgct
     7981 atggcttacc agcaccggtg ggttatgcct gtactactcc gcgcgaagca gaagaagccg
     8041 cttcaaaaat cggtgccggc ccgtgggtag tgaaatgtca ggttcacgct ggtggccgcg
     8101 gtaaagcggg cggtgtgaaa gttgtaaaca gcaaagaaga catccgtgct tttgcagaaa
     8161 actggctcgg caagcgtctg gtaacgtatc aaacagatgc caatggccaa ccggttaacc
     8221 agattctggt tgaagcagcg accgatatcg ctaaagagct gtatctcggt gccgttgtag
     8281 accgtagttc ccgtcgtgtg gtctttatgg cctccaccga aggcggcgtg gaaatcgaaa
     8341 aagtggcgga agaaactccg cacctgatcc acaaagttgc gcttgatccg ctgactggcc
     8401 cgatgccgta tcagggacgc gagctggcgt tcaaactggg tctggaaggt aaactggttc
     8461 agcagttcac caaaatcttc atgggcctgg cgaccatttt cctggagcgc gacctggcgt
     8521 tgatcgaaat caacccgctg gtcatcacca aacagggcga tctgatttgc ctcgacggca
     8581 aactgggcgc tgacggcaac gcactgttcc gccagcctga tctgcgcgaa atgcgtgacc
     8641 agtcgcagga agatccgcgt gaagcacagg ctgcacagtg ggaactgaac tacgttgcgc
     8701 tggacggtaa cattggttgt atggttaacg gcgcaggtct ggcgatgggt acgatggaca
     8761 tcgttaaact gcacggcggc gaaccggcta acttccttga cgttggcggc ggcgcaacca
     8821 aagaacgtgt aaccgaagcg ttcaaaatca tcctctctga cgacaaagtg aaagccgttc
     8881 tggttaacat cttcggcggt atcgttcgtt gcgacctgat cgctgacggt atcatcggcg
     8941 cggtagcaga agtgggtgtt aacgtaccgg tcgtggtacg tctggaaggt aacaacgccg
     9001 aactcggcgc gaagaaactg gctgacagcg gcctgaatat tattgcagca aaaggtctga
     9061 cggatgcagc tcagcaggtt gttgccgcag tggaggggaa ataatgtcca ttttaattga
     9121 taaaaacacc aaggttatct gccagggctt tactggtagc caggggactt tccactcaga
     9181 acaggccatt gcatacggca ctaaaatggt tggcggcgta accccaggta aaggcggtac
     9241 tacccacctc ggcctgccgg tgttcaacac cgtgcgtgaa gctgttgctg ctactggcgc
     9301 taccgcttct gttatctacg taccagcacc gttctgcaaa gactccattc tggaagctat
     9361 tgatgcaggc atcaaactga ttatcaccat cactgaaggc atcccgacgc tggatatgct
     9421 gactgtgaaa gtgaaactgg acgaagccgg cgttcgtatg atcggcccga actgcccggg
     9481 cgttatcact ccgggtgaat gcaaaatcgg tatccagccg ggtcacattc acaaaccggg
     9541 taaagtgggt atcatttccc gttccggtac attgacctat gaagcggtta aacagaccac
     9601 ggattacggt ttcggtcagt cgacctgtgt tggtatcggc ggtgacccga tccctggctc
     9661 taactttatc gacattctcg aaatgttcga aaaagatccg cagaccgaag cgatcgtgat
     9721 gatcggtgag atcggcggta gcgctgaaga agaagcggca gcgtacatca aagagcatgt
     9781 taccaagcca gttgtgggtt acatcgctgg tgtgactgcg ccgaaaggca aacgtatggg
     9841 ccacgcgggt gccatcattg ccggtgggaa agggactgcg gatgagaaat tcgctgctct
     9901 ggaagccgca ggcgtgaaaa ccgttcgcag cctggcggat atcggtgaag cactgaaaac
     9961 tgttctgaaa taaatatctg taataagaaa tagccctcgc cgcttccctc tacaggaatg
    10021 gcgaagggct gtcggtttcg acatggttgg ccatcgtatg atggcctttt ttgtgcttat
    10081 cgcgatgatt ttcgttgcgc tatcagggta aatttatagt catcggtatt aaaagcgttg
    10141 cggctatatt caaacacccg accatcaact aaatatccac gcgatacttt ttcaagaatc
    10201 ggctttgtct ggctgatatt aagcagacgg ctcatctctt cggttggcat cagaggaatg
    10261 atttcctgtt cgctacgatc gataaccatt ttcttcactt cttcaataaa gtgatatttc
    10321 gaattttcca tgacctgcca ggtgagatcc gggaacaacg caagcggcat ccaggtttct
    10381 tccagcgcca ttggcttttg cttgcgatag cgcacgcgct tcacatgcca cacacgatcc
    10441 tgcgcggtga tttgtagctg ttgctgaaga aaatcgtcag ccggaatcac ttcaaatatc
    10501 aggacttcac tgtgtgtatc gacgtgacgg tccgacagtt tttcatcaaa actggttaac
    10561 tgaaaaatat cgtaattgac ccgctcttct ttgacgtaag tcccgctgcc ctgaatgctt
    10621 tcgaggatct gttgctcgac taactggcgc aaagcctgac gcaccgtaac ccggctgacg
    10681 ccaaactctg tttgtaatgc cgattcggta ggtaacgcat cgccaggttt aagctcgcca
    10741 cgcgcaattt gttcacgaat gcgatcggca atctgccggt ataagggctt gtgtcccatt
    10801 tttagtatct cattaatacg aatttagcca ttatgccc
//
LOCUS       DAJSKJ010000130        10770 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000130 DAJSKJ010000000
VERSION     DAJSKJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.131"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            257..2962
                     /gene="malT"
                     /locus_tag="ON345_002895"
     CDS             257..2962
                     /gene="malT"
                     /locus_tag="ON345_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR3072929.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALILANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3005..4020)
                     /gene="rtcA"
                     /locus_tag="ON345_002896"
                     /pseudo
     CDS             complement(3005..4020)
                     /gene="rtcA"
                     /locus_tag="ON345_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(4024..5250)
                     /gene="rtcB"
                     /locus_tag="ON345_002897"
     CDS             complement(4024..5250)
                     /gene="rtcB"
                     /locus_tag="ON345_002897"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR3072930.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5439..7037
                     /gene="rtcR"
                     /locus_tag="ON345_002898"
                     /pseudo
     CDS             5439..7037
                     /gene="rtcR"
                     /locus_tag="ON345_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(7019..7777)
                     /locus_tag="ON345_002899"
     CDS             complement(7019..7777)
                     /locus_tag="ON345_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR3072931.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7794..8624)
                     /gene="glpG"
                     /locus_tag="ON345_002900"
     CDS             complement(7794..8624)
                     /gene="glpG"
                     /locus_tag="ON345_002900"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR3072932.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8669..8995)
                     /gene="glpE"
                     /locus_tag="ON345_002901"
     CDS             complement(8669..8995)
                     /gene="glpE"
                     /locus_tag="ON345_002901"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR3072933.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9185..10690
                     /gene="glpD"
                     /locus_tag="ON345_002902"
     CDS             9185..10690
                     /gene="glpD"
                     /locus_tag="ON345_002902"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR3072934.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT         2563 a         2967 c         2776 g         2464 t
ORIGIN      
        1 tttcatcagg aatgaagaag aaacccttgc ttaaatgaat ctgatgaaca taagggaaac
       61 cagtattcac gctggatcag cgtcgtttta ggtgagttgt taataaagat ttggaattgt
      121 gacacagtgc aaattcagac acataaaaaa acgtcatcgc ttgcattaga aaggtttctg
      181 gccgacctta taaccattaa ttacgaagcg caaaaaaaat aatatttcct cattttctac
      241 agtgaagtga ttaactatgc tgattccgtc aaaactaagt cgtccggttc gactcgacca
      301 taccgtggtt cgtgagcgcc tgctggctaa actttccggc gcgaacaact tccggctggc
      361 gctgatcacg agtcctgcgg gctacggaaa gaccaccctc atttcccagt gggcggcagg
      421 caaaaacgat atcggctggt actcgctgga tgaaggtgat aaccagcaag agcgtttcgc
      481 cagctatctc attgccgccg tgcagcaggc aaccaacggt cactgtgcga tatgtgagac
      541 gatggcgcaa aaacggcaat atgccagcct gacgtcactc ttcgcccagc ttttcattga
      601 gctggcggaa tggcatagcc cactttatct ggtcatcgat gactatcatc tgatcactaa
      661 tccagtgatc cacgagtcaa tgcgcttctt tattcgccat caaccagaaa atctcaccct
      721 ggtggtgttg tcacgcaacc ttccgcaact gggcattgcc aatctgcgtg ttcgtgacca
      781 actgctggaa attggcagtc agcaactggc atttacccat caggaagcga agcagttttt
      841 tgattgccgt ctgtcatcgc cgattgaagc cgcagaaagc agtcggattt gcgatgacgt
      901 ttccggttgg gcgacggcac tacagctaat cgccctctcc gcccggcaga atacccactc
      961 agcccataag tcggcacgcc gcctggcggg aatcaatgcc agccatcttt cggattatct
     1021 ggtcgatgag gttttggata acgtcgatct cgcaacgcgc cattttctgt tgaaaagcgc
     1081 cattttgcgc tcaatgaacg atgccctcat cacccgtgtg accggcgaag aaaacgggca
     1141 aatgcgtctc gaagagattg agcgtcaggg gctgttttta cagcggatgg atgataccgg
     1201 cgagtggttc tgctatcacc cgctgtttgg caacttcctg cgccagcgct gccagtggga
     1261 actggcggcg gagctgccgg aaatccaccg tgccgccgca gaaagctgga tggcccaggg
     1321 attccccagc gaagcgattc atcatgcgct ggcggcaggc gatgcgctga tgctgcgcga
     1381 tattctgctt aatcacgcct ggagtttgtt caaccatagc gaactgtcgc tgctggaaga
     1441 gtcgcttaag gccctgccgt gggacagttt gctggaaaat ccgcagttag tgttattgca
     1501 ggcgtggcta atgcaaagcc aacatcgcta tggcgaagtt aacaccctgc tagcccgtgc
     1561 tgaacatgaa atcaaggaca tcagagaagg caccatgcac gcagaattta acgctctgcg
     1621 cgcccaggtg gcgattaacg atggtaatcc ggatgaagcg gaacggctgg caaaactggc
     1681 actggaagag ctgccgccgg gctggttcta tagccgcatt gtggcaacct cggtgctggg
     1741 tgaagtgctg cactgcaaag gcgaattgac ccgctcactg gcgctaatgc agcaaaccga
     1801 acagatggca cgccagcacg acgtctggca ctacgcctta tggagtttaa tccagcaaag
     1861 tgaaattctg tttgcccaag ggttcctgca aaccgcgtgg gaaacacagg aaaaagcgtt
     1921 ccagctgatc aacgagcagc atctggaaca gctgccaatg catgagtttc tggtgcgcat
     1981 ccgagcacaa ctgttatggg cctgggcacg gctggatgaa gccgaagcgt cggcgcgtag
     2041 cgggattgaa gtcttgtcgt cttatcagcc acagcaacag cttcagtgcc tggcaatgtt
     2101 gattcaatgc tcgctggccc gtggtgattt agataacgcc cgtagccagc tgaaccgtct
     2161 ggaaaacctg ctggggaatg gcaaatatca cagcgattgg atctctaacg ccaacaaagt
     2221 gcgggtgatt tactggcaaa tgaccggcga taaagccgcc gctgccaact ggttgcgtca
     2281 tacggctaaa ccagagtttg cgaacaacca cttcctgcaa ggtcaatggc gcaacattgc
     2341 ccgtgcacaa atcttgctgg gcgagtttga accggcagaa attgttctcg aagaactcaa
     2401 tgaaaatgcc cggagtctgc ggttgatgag cgatctcaac cgtaacctgt tgctgcttaa
     2461 tcaactgtac tggcaggccg gacgtaaaag tgacgcccag cgcgtgttgc tggacgcatt
     2521 aatactggcg aatcgcaccg gatttatcag tcattttgtc atcgaaggcg aagcgatggc
     2581 gcaacaactg cgccagctga ttcaactcaa tacgctgccg gaactggaac agcatcgcgc
     2641 acagcgtatt ctgcgagaaa tcaatcaaca tcatcggcat aaattcgccc atttcgatga
     2701 gaatttcgtt gaacgtctgc taaatcatcc ggaagtgcca gaactgatcc gcaccagccc
     2761 attgacccaa cgtgaatggc aggtcctggg gctgatttac tccggttaca gcaatgaaca
     2821 aattgcgggc gaactggaag tggcggcaac caccatcaag acgcatatcc gcaatctgta
     2881 ccaaaaactc ggcgtcgccc accgtcaggc tgccgtgcag cacgcgcaga aactgctgaa
     2941 gatgatgggt tatggggtgt gagtttagcc ggataatgca ttggatccgg tttacatctt
     3001 tacatcattc aatactcacc cgcgttacgc catctgtttc tatcaaacca aaccgcaccg
     3061 gcaagaaacg ctccaccacc gcgatattgg tcagcagatg gcatgaagga tgggcgacct
     3121 taaattctcc cgcgcccgcc agcgccatcg gcagtaccag ttggtcggcg agatattccc
     3181 ccaccgccgc tgggcttgcc aggtagcgtt tcacctcttt cactaactgc gcggctacca
     3241 cctcggcact gacgcgcttt tcaccgacga cgaaaaagcg ttcagtgata ttttcacttt
     3301 cgacttccag cgagacggta ttacccggcc cctggtcgcg cggcaggtta tgaatattct
     3361 gttcatgcag ggaaaaactc cccgccagtg tagcgatttc acgctcagca acatggcgcg
     3421 gcacaccagc taatagaact tctccacaca tctgcacaat gttcccgcgc tcgccaagtt
     3481 gcaaggtgtt aaacgatgcc acgggcgaga cttccgttgc caccacaccg cctccggcag
     3541 gataaaaacc gtgacgtaac agcgtggttt gctgatgaat tcctattttc gccagcagcg
     3601 gctccagcac ccggcggata aaatccgcag gcggggccga cgggttatcg gtgcctccgc
     3661 tcacttcaac acgcgaaggt ccatcggcaa accacagcgc gggcagcacc gtttgcagca
     3721 ccagcgtaca gcttccggca ctaccgatag caaagcggta ctcgccgccg cgcacggtgc
     3781 caggccggaa gaccagacgc tgcgacccca gctccgcgcc ttcaccgttg ccctacaaat
     3841 ttccgcagcc gcttttaccg cggtcagatg ctggcgcaac agccccggtt tcgcccgccc
     3901 ggcacgaatg ctggtgatgg taaatggctg gccggttatc atcgacaggc tcagcgccga
     3961 gcgcaggatc tgcccgccac cttcgccctg tgcgccatcc agcgcaatca tccttttcat
     4021 catttatcct tttacgcaca ccacctgacg cagggtatag ataacttcca ccagatcgct
     4081 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
     4141 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tgatcttcca cgctgaacag
     4201 ttttttcgct ttagtacggc tcattacccg cccggcaccg tggctgcacg aacagaacga
     4261 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
     4321 tccatattga ccagcacgcg cagacaccgc gcctttacgc gtcacgtaga tctcttcacc
     4381 aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
     4441 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaat gccgttacca cgttttccat
     4501 catcgcatcg cggttaaggc tggcaaaaag ctgcgcccag gccacggctt tcaggtaatc
     4561 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
     4621 cgtttcctgc atctcttttt gtgccagatc gataaagtaa gtcccgatgg cgttaccaat
     4681 tccgcgtgaa ccggagtgca gcataatcca cacctggtcc gactcatcaa ggcagatttc
     4741 aataaagtgg ttaccggttc ccagcgttcc caggtgttta tagttattgg tattcaggaa
     4801 acggggatat ttttgcgtta accactgata accggcttca agctcagccc atttcgcatc
     4861 gacgttaaca ggcgggtttt cccatgcgcc tttatcacgt ttacaacggc cagtggtacg
     4921 cccgtgcggc acggccgttt caatcgcctg acgcagctct gccaggtttt caggcaggtc
     4981 ttccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
     5041 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
     5101 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
     5161 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
     5221 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgctgtc
     5281 gtgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt ttaattcatt
     5341 gtttttaaat aggttaaatc tttacgtccg taaacggaga ttttccacaa aaccaattca
     5401 ccgataatga aatatcgtct tttataagga tatctaagat gcgtaaaaca gtggcttttg
     5461 gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcgaaatgac
     5521 gtccgacgct ttgtttatgc cagcaagaat cgttagtcat cgatcggctg gaattgttgc
     5581 acgacacccg ctcgcgctcg ctattcgaaa cgcttaaacg cgatatcgcc agcgtttcgc
     5641 cagaaacaga agtcgtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg
     5701 tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact
     5761 atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg
     5821 aagcgcgtta cctgcccgcc cggctgatac aatcttcacc tccgcgcaaa aaagaacagc
     5881 cgcgcggcgc aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg
     5941 ccagccgctt tgccgaggaa cgccagcaaa cgcttgattt tcttaagtcc ggcattgcca
     6001 cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg atcaaatccc
     6061 gcgcgccgat tctgcttaac ggtccaaccg gcgcgggcaa gtcatttctg gcgcgacgca
     6121 tcttcgagtt aaaacaggcg cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg
     6181 ccaccctgcg cggcgatacc gccatgtcga cgctgtttgg tcatgtaaaa ggcgcgttta
     6241 ccggggcgcg ggaatctcgt gaaggtttat tacgcagcgc caacggcgga atgttgtttc
     6301 ttgatgagat tggcgaactg ggcgcagacg aacaagcaat gctgctaaaa gccattgaag
     6361 agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg
     6421 ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaatttcgc gaagatctgt
     6481 acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata
     6541 ttgaaccaaa ccttgattat gaagtggagc gccacgccac actcactggc gacagcgtgc
     6601 gttttaacac cgaagcgcgg cgcgcctggt tggcttttgc gacctctccc caggcaacat
     6661 ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta
     6721 gcggacgcat cactctggac gtggttgaag atgagataaa ccgtctgcgc tataactggc
     6781 aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgacc
     6841 tttttgacag gctgcaactg gaacacgtga tagctctctg tcgccaggca aagtcgcttt
     6901 ccgctgccgg acgcctgctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg
     6961 cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc
     7021 agcacagctc cagttgaata tgatggtccg tcagcacctg cattacgctt actggcggcg
     7081 gggcgtcggt gtagacggca tctaccatgc tgatgctgcc catattgacc atcgcgttac
     7141 ggccaaattt cgagtgatcg acaaccagca taacgtggcg cgagttctca ataatggcgc
     7201 gcttggtgcg aacttcgtgg taatcgaact ccagcagcga gccgtcgcta tcgatgccgc
     7261 ttatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc
     7321 caatgatccc gccatcgcgg ctgcgtaatt cgccaccggc gagaataatg cggaaatctt
     7381 cttttaccat caacgtgtta gcaacattga gattgttggt gacaatacgc aaattgctgt
     7441 gattaagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagtgtcg
     7501 agccgttggg gatttgctcc gccactttgc gggcaatcct ctctttttct tcggtctggg
     7561 tggctttgcg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcaccgccat
     7621 ggtgacgcag gatcaggttt tgctccgcca gctcattgag gtcgcggcga atagtctgcg
     7681 ggctgacgga gaaatgctct accagctctt cagtactgac ataaccctgc tgtttaacca
     7741 gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt
     7801 cgttttcgcg cattgagcga atcaacaaaa gccatcgcta accccacggc taacccggcg
     7861 atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag
     7921 atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttca
     7981 ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac
     8041 ggcccgctga atttttgctg cacatagccg cttaacaggg cgctaatgag agtaataaca
     8101 attagcttac cgctaccgag gcgtttttcc accgcaccgc cgagatacca ccaccagagc
     8161 aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag
     8221 aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg
     8281 ccgagaattt gcatggcgat aaacaccacc acgcaggcga tcatcaccac ccaggttacc
     8341 ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg cagaccactg
     8401 ccggtatggc ccgcctgcca gctggccgcc agataacgcg gatctgccgg gttttcgaga
     8461 aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg
     8521 ctttggttat gttgttgaat cgtaaggata acaccctgcg tcgccatgta atcaacaaac
     8581 gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat
     8641 tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact
     8701 gacgctgcca ggcttcaaaa ccgccgtcaa tgctatagac cacatcgtag ccctgttgca
     8761 gcagatactg cgccgcgcct ttgctgctat tgccgtgata acacatcacc atcaccggag
     8821 tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct
     8881 gcaccgcatg tcccattgcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt
     8941 tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaacaga tccatgcgtc
     9001 tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat
     9061 aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac ctaaagcgcg
     9121 attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg
     9181 cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc
     9241 ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg
     9301 tgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta
     9361 tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggcccc
     9421 gcatatcgcc ttcccgatgc gttttcgcct gccgcatcgt ccgcatctgc gcccggcgtg
     9481 gatgattcgg attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg
     9541 atcaaccggt ttgcgttttg gcgcaaactc agtgttaaaa ccggaaatta agcgcggatt
     9601 cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt
     9661 ggtgcgtaaa ggcggcgaag tgcttactcg tactcgtgcc acttctgctc gccgcgaaaa
     9721 cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaataca cctggcaggc
     9781 gcgcggcctg gttaatgcta ccggcccgtg ggtgaaacag ttcttcgacg acgggatgca
     9841 tctgccctca ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt
     9901 gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat
     9961 cccgtggatg gacgagtttt caatcatcgg caccaccgat gtggaataca aaggcgatcc
    10021 aaaagcggtg aagattgaag agagtgaaat caactacctg ctgaaagtgt ataacgcgca
    10081 ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct
    10141 gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca
    10201 tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg
    10261 caaactggcg gaacatgcgc tggaaaaact gacgccgtat tatcagggca ttggcccggc
    10321 gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc
    10381 cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcgcgtc attacgcacg
    10441 cacttacggc agcaacagcg agctgctgct cggcaatgcg ggggcgataa gcgacctcgg
    10501 ggaggatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg
    10561 ggtccgccgc accgacgatg ccctttggcg tcgcacaaaa caaggcatgt ggctgaatgc
    10621 ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct
    10681 ggcgtcgtaa ggatgatgcc cggttgaagt gcaaccgggc tatttggatt ccttagacta
    10741 ttcattgtaa ataattgaat aacggcccct
//
LOCUS       DAJSKJ010000131        10517 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000131 DAJSKJ010000000
VERSION     DAJSKJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.132"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(1..202)
                     /locus_tag="ON345_002903"
                     /pseudo
     CDS             complement(1..202)
                     /locus_tag="ON345_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
     gene            326..1330
                     /gene="argF"
                     /locus_tag="ON345_002904"
     CDS             326..1330
                     /gene="argF"
                     /locus_tag="ON345_002904"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3072935.1"
                     /translation="MSDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLT
                     GKNIALIFEKDSTRTRCSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGHGQEVVETLAQYAGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTED
                     VAAGVKGADFIYTDVWVSMGEAKEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLP
                     AFHDDQTTLGKQMAKEFDLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMMATLGE
                     "
     gene            1469..2227
                     /gene="xynR"
                     /locus_tag="ON345_002905"
     CDS             1469..2227
                     /gene="xynR"
                     /locus_tag="ON345_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor XynR"
                     /protein_id="HCR3072936.1"
                     /translation="MPIIQSVERALQILDLFNEQATELKITDISKLMGLSKSTLHSLL
                     KTLQLHGYIDQNPENGKYRLGMKLVERGHFVVGSIDIRQKAKGWLTELSRRTGQTTHL
                     GILDGREGVYIEKIEGKLAAIAYSRIGRRLPVHATAIGKVLIAWLGEAELNALLEGYQ
                     YTTFTPATLASREALMSALAQTREQGYALDSEENEQGVRCVAVPVWNHESRVIAALSL
                     STLTSRVDDAELANFREQLQQAGLALSRALGYPA"
     gene            complement(2232..3842)
                     /locus_tag="ON345_002906"
     CDS             complement(2232..3842)
                     /locus_tag="ON345_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3072937.1"
                     /translation="MEITNPILTGFNPDPSLCRQGEDYYIATSTFEWFPGVRIYHSRD
                     LKNWSLVSTPLDRVSMLDMKGNPDSGGIWAPCLSYADGKFWLLYTDVKIVDSPWKNGR
                     NFLVTAPSIEGPWSEPIPMGNGGFDPSLFHDDDGRKYYIYRPWGPRHHSNPHNTIVLQ
                     AFDPQTGTLSPERKTLFTGTPLCYTEGAHLYRHAGWYYLMVAEGGTSYEHAVVVLRSK
                     NIDGPYELHPDVTMMTSWHLPENPLQKSGHGSLLQTHTGEWYMAYLTSRPLRLPGVPL
                     LASGGRGYCPLGRETGIARIEWRDGWPYVEGGKHAQLTVKGPQVAEQPAAVPGNWRDD
                     FDASSLDPELQTLRIPFDDTLGSLTARPGFLRLYGNDSLNSTFTQSTVARRWQHFAFR
                     AETRMEFSPVHFQQSAGLTCYYNSKNWSYCFVDYEEGQGRTIKVIQLDHNVPSWPLHE
                     QPIPVPEHAESVWLRVDVDTLVYRYSYSFDGETWHTVPVTYEAWKLSDDYIGGRGFFT
                     GAFVGLHCEDISGDGCYADFDYFTYEPV"
     gene            complement(3854..5236)
                     /locus_tag="ON345_002907"
     CDS             complement(3854..5236)
                     /locus_tag="ON345_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3072938.1"
                     /translation="MTQLTMKDKIGYGLGDTACGFVWQATMFLLAYFYTDVFGLSAGI
                     MGTLFLVSRVLDAVTDPLMGLLVDRTRTRHGQFRPFLLWGAIPFGIVCVLTFYTPDFS
                     AQGKIIYACVTYILLTLVYTFVNVPYCAMPGVITADPKERHALQSWRFFLAAAGSLAI
                     SGIALPLVSIIGKGDEQVGYFGAMCVLGLSGVVLLYVCFFTTKERYTFEVQPGSSVAK
                     DLKLLLGNSQWRIMCAFKMMATCSNVVRGGATLYFVKYVMDHPELATQFLLYGSLATM
                     FGSLCSSRLLGRFDRVTAFKWIIVAYSLISLLIFVTPAEHIALIFALNILFLFVFNTT
                     TPLQWLMASDVVDYEESRSGRRLDGLVFSTYLFSLKIGLAIGGAVVGWILAYVNYSAS
                     SSVQPVEVLTTIKILFCVVPVVLYAGMFIMLSLYKLTDARVEAISRQLIKHRAAQGEA
                     VPDAATAASH"
     gene            complement(5463..7430)
                     /gene="yagF"
                     /locus_tag="ON345_002908"
     CDS             complement(5463..7430)
                     /gene="yagF"
                     /locus_tag="ON345_002908"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YagF"
                     /protein_id="HCR3072939.1"
                     /translation="MTIEKIFTPQDDAFYAVITHAAGPQGALPLTPQMLMESPSGNLF
                     GMTQNAGMGWDANKLTGKEVLIIGTQGGIRAGDGRPIALGYHTGHWEIGMQMQAAAKE
                     ITRNGGIPFAAFVSDPCDGRSQGTHGMFDSLPYRNDAAIVFRRLIRSLPTRRAVIGVA
                     TCDKGLPATMIALAAMHDLPTILVPGGATLPPTVGEDAGKVQTIGARFANHELSLQEA
                     AELGCRACASPGGGCQFLGTAGTSQVVAEALGLALPHSALAPSGQAVWLEIARQSARA
                     VSELDSRGITTRDILSDKAIENAMVIHAAFGGSTNLLLHIPAIAHAAGCTIPDVEHWT
                     RINRKVPRLVSVLPNGPDYHPTVRAFLAGGVPEVMLHLRDLGLLHLDAMTVTGQTVGE
                     NLEWWQASERRARFRQCLREQDGVEPDDVILPPEKAKAKGLTSTVCFPTGNIAPEGSV
                     IKATAIDPSVVGEDGVYHHTGRVRVFVSEAQAIKAIKREEIVQGDIMVVIGGGPSGTG
                     MEETYQLTSALKHISWGKTVSLITDARFSGVSTGACFGHVSPEALAGGPIGKLRDNDI
                     IEIAVDRLTLTGSVNFIGTADNPLTPEEGARELARRQTHPDLHAHDFLPDDTRLWAAL
                     QSVSGGTWKGCIYDTDKIIEVINAGKKALGI"
     gene            complement(7445..8353)
                     /gene="yagE"
                     /locus_tag="ON345_002909"
     CDS             complement(7445..8353)
                     /gene="yagE"
                     /locus_tag="ON345_002909"
                     /EC_number="4.1.2.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate aldolase"
                     /protein_id="HCR3072940.1"
                     /translation="MPQSALFTGIIPPVSTIFTADGQLDKPGTAALIDDLIKAGVDGL
                     FFLGSGGEFSQLGAEERKAIARFAIDHVDRRVPVLIGTGGTNARETIELSQHAQQAGA
                     DGIVVINPYYWKVSEANLIRYFEQVADSVTLPVMLYNFPALTGQDLTPALVKTLADSR
                     SNIIGIKDTIDSVAHLRSMIHTVKGAHPHFTVLCGYDDHLFNTLLLGGDGAISASGNF
                     APQVSVNLLKAWRDGDVAKAAGYHQTLLQIPQMYQLDTPFVNVIKEAIVLCGRPVSTH
                     VLPPASPLDEPRKAQLKTLLQQLKLC"
     gene            8649..9803
                     /locus_tag="ON345_002910"
     CDS             8649..9803
                     /locus_tag="ON345_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISEc19 family transposase"
                     /protein_id="HCR3072941.1"
                     /translation="MESLMPWDARDTMSLRTEFVLFASQDGANIRSLCRRFGISPATG
                     YKWLQRWAQEGAAGLQDRPRIPHHSPNRSSDDITALLRMAHDRHERWGARKIKRWLED
                     QGHTMPAFSTVHNLMARHGLLPGASPGIPATGRFEHDAPNRLWQMDFKGHFPFGGGRC
                     HPLTLLDDHSRFSLCLAHCTDERRETVQQQLVSVFERYGLPDRMTMDNGSPWGDTTGT
                     WTALELWLMRLGIRVGHSRPYHPQTQGKLERFHRSLKAEVLQGKWFADSGELQRAFDH
                     WRTVYNLERPHEALDMAVPGSRYQPSARQYSGNTTPPEYDEGVMVRKVDISGKLSVKG
                     VSLSAGKAFRGERVGLKEMQEDGSYEVWWYSTKVGVIDLKKKSITMGKGC"
     gene            9897..10247
                     /gene="yagB"
                     /locus_tag="ON345_002911"
     CDS             9897..10247
                     /gene="yagB"
                     /locus_tag="ON345_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system antitoxin YagB"
                     /protein_id="HCR3072942.1"
                     /translation="MNKDSTQTWGLKRDITPCFGARLVQEGHRLHFLADRAGFTGSFS
                     EVQTLQLDEAFPHFVAHLELMLLSCELNPRYAHCVTLYRNGLTGEADTLGSHGYVYIA
                     ILNRHGFNRHLRVI"
     gene            10270..>10517
                     /locus_tag="ON345_002912"
     CDS             10270..>10517
                     /locus_tag="ON345_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3072943.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDLGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDILKKA"
BASE COUNT         2138 a         3098 c         3264 g         2017 t
ORIGIN      
        1 taacagatat atcgcctcca tcaccgctga agtaatgaag taaggcagca agcctcgcca
       61 tgttctccgc cattttagaa gcataatcct taaaatcata aagaaggcca atcattctca
      121 tttctgactc gacctggttg tagaattcga tccaatgtct ttctgcttct gcagagaatc
      181 ggaggcagat acgattattt tcacacacgg acgggtttgc ctccaccttt gtaagaaaga
      241 attgtgaaat ggggttgcaa atgaataatt acacatataa ggtgaatttt aattcaataa
      301 gtggcgttcg ccatgcgagg ataaaatgtc cgatttatac aaaaaacact ttctgaaact
      361 gctcgacttt acccctgcac agttcacttc tctgctgacc cttgccgcac agctcaaagc
      421 cgataaaaaa aatggcaagg aagtacagaa gcttaccggt aaaaacatcg cgctcatctt
      481 cgaaaaagac tcgactcgta cccgttgctc tttcgaagtt gccgcatttg accagggcgc
      541 gcgcgttacc tatttagggc cgagcggcag ccagattggg cataaagagt caattaagga
      601 caccgcgcgg gttctcgggc ggatgtatga cggcattcag tatcgcggtc acggccagga
      661 agtggtcgaa acgctggcgc agtatgcggg cgtgccggtg tggaacgggc tgaccaacga
      721 gttccacccg acccagctgc tggcggacct gatgaccatg caggagcacc tgccgggcaa
      781 ggcgtttaac gagatgacgc tggtctacgc gggcgatgcg cgcaacaaca tgggcaactc
      841 gatgctggaa gcggcggcgc tgaccgggct ggatctgcgc ctgttggccc cgaaagcctg
      901 ctggccggaa gagagcctgg tggcggagtg cagcgcgctg gcggagaagc acggcgggaa
      961 aattactctg acggaagacg tggcggcagg cgttaagggc gcggacttta tctataccga
     1021 cgtgtgggtg tcgatgggcg aggccaaaga gaagtgggca gagcggattg cgctgctgcg
     1081 cgggtatcag gtgaacgcgc agatgatggc gctgaccgac aacccgaacg tgaagttcct
     1141 gcactgtctg ccggcgttcc atgacgacca gactacgctc ggcaagcaga tggcgaagga
     1201 gttcgatctg cacggcggga tggaggtgac ggacgaggtg tttgagtcgg cggcgagcat
     1261 cgtgttcgac caggcggaaa accggatgca tacgattaag gcggtgatga tggcaacgct
     1321 tggggagtga ttgggtccgc tgcgcgttgg tgccctcacc ccggccctct cccacaggga
     1381 gagggagaac accggctcca tttcattgat ttttcatccc gaaaaaggta cgttttcgcc
     1441 ttaattccag cgtggacatg ccagcattat gccgattatt cagtctgttg aacgtgcgtt
     1501 gcagatcctc gacctgttca acgagcaggc caccgagctt aagatcaccg acatcagcaa
     1561 actgatgggg ctgagcaaga gtaccctcca ctcgctgcta aaaaccctgc agcttcacgg
     1621 ctatatcgat cagaacccgg agaacggcaa gtatcgcctc ggcatgaagc tggtcgagcg
     1681 cggccatttt gtcgtgggct ccatcgatat tcggcagaag gcaaaaggct ggctgacgga
     1741 gctgtcccgg cggaccgggc agaccaccca tctggggatc ctggacgggc gtgaaggggt
     1801 ctatatcgag aagattgaag gcaagctggc cgccatcgcc tattcacgca tcggccgccg
     1861 cctgccggtg cacgccaccg ccatcggcaa ggtgttgatt gcctggctgg gcgaggccga
     1921 gctgaacgcc ctgctggagg gctatcagta cactaccttt acgcccgcca ccctcgcgtc
     1981 tcgcgaagcc ttaatgagcg ccctggcgca gacccgcgag caaggctacg ccctggacag
     2041 cgaagagaac gagcagggcg tgcgctgcgt ggcggtgccg gtgtggaacc acgagtcccg
     2101 cgtcatcgcc gccctgagcc tgtcgacgct gacctcccgc gtggacgacg cggagctggc
     2161 taatttccgc gagcagcttc agcaggccgg gctcgcgctc tcgcgcgcgc tgggctaccc
     2221 ggcctgagcc gttagaccgg ctcgtaggtg aagtagtcga agtccgcgta gcagccgtcg
     2281 ccgctgatgt cctcgcagtg caggcccaca aacgcgccgg tgaagaagcc gcgcccgccg
     2341 atgtagtcgt ccgacagctt ccacgcctca tacgtcaccg gcacggtgtg ccacgtctcg
     2401 ccatcaaacg agtagctgta gcggtagacc agcgtatcca cgtccacccg cagccagacg
     2461 ctctccgcat gttccggcac cggaatgggc tgctcgtgca gcggccacga cggcacgttg
     2521 tggtcgagct ggataacttt gatggttcta ccctgtccct cctcgtagtc cacaaagcag
     2581 tagctccagt ttttgctgtt gtagtagcag gtcagccccg cgctctgctg gaagtgcacc
     2641 ggcgagaact ccatccgcgt ttctgcccgg aaggcgaagt gctgccagcg gcgcgccacg
     2701 gtcgattggg tgaaggtcga attgagcgag tcgttgccat agagccgtaa gaagcccggg
     2761 cgcgcggtga gcgagccgag ggtgtcgtcg aacggaatgc gcagggtctg cagctccggg
     2821 tcaagcgaac tggcgtcgaa atcgtcccgc cagttgcccg gaacggctgc aggctgctcg
     2881 gctacttgcg ggcctttcac ggtcagctgc gcgtgcttgc cgccttccac gtacggccag
     2941 ccgtcgcgcc attcaatgcg ggcgatgccg gtctcgcgcc ccagcgggca gtagccgcgt
     3001 ccgccggagg ccagcagcgg cacgccgggc aggcgcagcg ggcggctggt gaggtaggcc
     3061 atgtaccatt cacccgtatg cgtctgcagc agcgagccgt ggccgctctt ctgcagcggg
     3121 ttctccggca ggtgccagct ggtcatcatc gttacgtccg ggtgcagctc gtacggcccg
     3181 tcgatatttt tggaacgcag caccacgacg gcgtgctcgt agctggtgcc gccctcggcg
     3241 accatcaggt agtaccatcc cgcgtggcga tacaggtgcg cgccttcggt gtagcagagc
     3301 ggcgtgccgg taaacagcgt tttgcgctcg ggcgagagcg tgccggtctg cgggtcaaac
     3361 gcctgtaaca cgatggtgtt gtgcgggttg ctgtggtggc gcggccccca cgggcggtag
     3421 atatagtatt tgcggccatc gtcgtcgtgg aacagggacg ggtcaaaccc gccgttgccc
     3481 atcgggattg gctcgctcca tggcccctcg atggagggcg cggtgacgag gaagttgcgg
     3541 ccgtttttcc acggcgagtc gacaatcttc acgtcggtgt agagcagcca gaatttaccg
     3601 tcggcgtagc tcaggcacgg cgcccagatg ccgccggagt ccgggttgcc cttcatgtcc
     3661 agcatcgaca cgcggtccaa cggggtgctg accagcgacc agtttttcag gtcacgggag
     3721 tggtagatgc gcacgcccgg gaaccactcg aaggtcgagg tggcgatgta gtagtcctcg
     3781 ccctggcggc acagggacgg gtccgggttg aagccggtga gtatcgggtt agtgatttcc
     3841 atattgcctc cggttaatgg gatgcggctg tcgcggcgtc gggaacggcc tcgccctgcg
     3901 ccgcgcggtg cttaatcagc tgccggctga tggcctccac gcgggcatcg gtgagcttgt
     3961 agagcgacag catgatgaac atgcccgcgt agagcaccac cggcaccacg cagaacagaa
     4021 ttttgatggt ggtgagcacc tcaaccggct gcacgctgct gctggcggaa tagttgacgt
     4081 acgccaggat ccagcccacc accgccccgc caatcgccag gccaatcttc aggctgaaca
     4141 ggtaggtgga gaacaccagc ccgtcgaggc ggcgaccgct gcggctctcc tcgtagtcca
     4201 ccacgtcaga agccatcagc cactgcagcg gcgtggtggt attaaagacg aacaggaaca
     4261 ggatgttgag ggcaaaaatg agcgcgatgt gctccgccgg ggtgacgaaa atcagcaggc
     4321 tgatcagcga gtaggcgacg atgatccact tgaaggcggt gacgcggtcg aagcggccca
     4381 gcaggcgtga ggagcaaagc gagccgaaca tggtggcgag gctgccgtaa agtaaaaact
     4441 gggtcgccaa ctccgggtga tccatcacgt atttcacgaa gtagagcgtc gccccgccgc
     4501 gcaccacgtt ggagcaggtc gccatcatct tgaacgcgca catgatgcgc cactggctgt
     4561 tgcccagcag cagcttaagg tctttcgcca ccgacgagcc cggctgcacc tcaaaggtgt
     4621 agcgctcttt ggtcgtgaag aagcagacgt agagcagcac cacgccgctc agccccagca
     4681 cgcacatggc gccgaagtag cccacctgct cgtccccttt gccgatgatg ctcaccagcg
     4741 gcagcgcgat gccgctgata gcgagcgagc ccgccgccgc caggaagaag cgccaggact
     4801 gcagggcgtg acgctctttc gggtcggcgg tgatgacgcc cggcatggcg cagtacggca
     4861 cgttaacgaa ggtgtagacc agggtcagga gaatgtaggt cacgcaggcg tagatgatct
     4921 tgccctgtgc ggagaagtcc ggcgtgtaga aggtcagcac gcagacgatg ccgaacggga
     4981 tggcccccca cagcaggaac gggcggaact ggccgtgccg cgtgcgggtg cggtctacca
     5041 gcagccccat cagcgggtcg gtgacggcgt cgagcacgcg ggagaccaaa aacagcgtgc
     5101 ccataatccc cgccgacagg ccgaagacgt cggtgtagaa ataggccagc aggaacatcg
     5161 tggcctgcca gacgaagccg caggcggtgt ctcccagccc gtagccaatt ttgtctttca
     5221 tggttaattg cgtcatcaga attacaccct tatgtagtgt gccgaattac aacatcgtgt
     5281 taatggagtg taatgagcta ttcaattaac cagcaatggt catatcttat ttccgaatta
     5341 cgttattttg cttttgtgac gccaacgata tatctcaact aattgtttaa attaataaat
     5401 ttcaaaaaca acaaagccgt ctgcgggaaa tattccgcag acggttttat ctcttaaaat
     5461 aattaaattc cgagcgcttt tttaccggcg ttaattacct cgataatttt atcggtgtca
     5521 taaatacagc ctttccaggt gccgccgctc accgactgca gtgccgccca cagccgggtg
     5581 tcgtccggca aaaagtcgtg ggcgtgcagg tccgggtgcg tctgccgccg cgccagctcg
     5641 cgcgcgccct cttccggcgt cagcgggttg tccgcggtgc cgatgaagtt cacgctgcca
     5701 gttaacgtca gacgatccac ggcaatctcg atgatgtcgt tatcgcgcag cttgccaatc
     5761 ggcccgcccg ccagcgcctc cggcgacacg tggccgaagc aggcgcccgt cgacacgccc
     5821 gagaagcgcg catcggtgat gagcgacacc gtcttgcccc acgagatatg ctttagcgcg
     5881 gaggtgagct ggtaggtctc ttccatgccg gtgccggacg gcccgccgcc gatcaccacc
     5941 atgatatcgc cctgcacaat ctcttcccgc ttgatcgcct tgatcgcctg cgcttccgag
     6001 acaaacaccc gcacccggcc ggtgtggtgg tatacgccat cttcgcccac caccgacggg
     6061 tcgatcgccg tggccttgat caccgaacct tccggcgcga tgttgcccgt cgggaagcag
     6121 accgtcgagg tcagcccttt cgcttttgcc ttctccggcg gcaggatcac gtcatccggc
     6181 tctacgccgt cctgctcgcg caggcactgg cggaagcgcg cccggcgctc ggacgcctgc
     6241 caccattcaa ggttctcgcc caccgtctgg ccggtcacgg tcatggcgtc cagatgcagc
     6301 aggccgaggt cgcgcaggtg gagcatcacc tccggcacgc cgcccgcgag gaaggcgcgc
     6361 acggtcgggt gatagtccgg gccgttgggc agcacgctca ccagacgcgg cactttacgg
     6421 ttgatgcgcg tccagtgctc aacgtccggg atcgtgcagc ccgccgcgtg ggcgatggcc
     6481 ggaatgtgca gcagtaaatt ggtggagccg ccgaacgccg cgtggatcac catcgcgttt
     6541 tcgatggctt tatcggagag gatatcccgc gtggtgatgc cgcggctatc cagctcgctg
     6601 accgcgcgcg ccgactggcg ggcgatctcc agccacaccg cctgcccgga cggcgccagc
     6661 gcggagtgcg gcagcgccag acccagcgcc tccgcgacca cctgcgaggt gcccgccgtg
     6721 ccgaggaact gacacccgcc gcccggcgag gcgcaggcgc gacagcccag ttcggcggcc
     6781 tcctgcaggg agagttcgtg gttggcgaaa cgcgcgccga tggtctgcac cttgcccgcg
     6841 tcttccccga cggtcggcgg cagcgtcgcc ccgcccggca ccagaatagt cggcaggtcg
     6901 tgcatcgcgg ccagcgcaat catggtggcg ggcagccctt tatcgcaggt cgctacgccg
     6961 atcaccgccc gccgcgtcgg cagggagcgg atcaggcggc gaaacacgat cgccgcgtcg
     7021 ttgcggtacg gcagggaatc gaacataccg tgcgtgccct gcgagcgccc gtcgcacgga
     7081 tcgctgacga aggccgcgaa cgggatcccg ccattgcggg tgatctcctt cgccgccgcc
     7141 tgcatctgca tgccgatctc ccaatgcccg gtgtggtagc ccagcgcgat tgggcgtccg
     7201 tctccggcgc ggatgccgcc ctgagtgccg ataatcagca cctctttgcc ggtgagcttg
     7261 ttggcgtccc agcccatccc ggcgttctgc gtcatgccga acaggttgcc gctgggagat
     7321 tccatcagca tctgcggggt cagcggcaga gcgccctgcg gccccgccgc gtgggtgatc
     7381 accgcataaa acgcgtcgtc ctgcggggtg aaaattttct caatggtcat cgttatcgtc
     7441 cggctcagca aagcttgagc tgttgcagca gggttttcag ctgcgccttg cgcggctcgt
     7501 ccagcggcga ggcgggcggc agcacgtgcg tggagacagg acgaccgcag agcacgatcg
     7561 cctctttaat cacgttcaca aacggcgtat ccagctgata catctgcgga atttgcagca
     7621 aggtctgatg atacccggcc gctttcgcca cgtccccgtc gcgccaggct ttcagaagat
     7681 tcaccgacac ctgcggggca aagttgccgc tcgccgatat cgccccgtcg ccgccgagca
     7741 gcagggtatt gaacagatga tcgtcgtagc cgcagagcac ggtgaagtgc ggatgggcac
     7801 ctttgacggt atggatcatg ctgcgcaggt gggcgacgga gtcaatggtg tctttgatgc
     7861 cgataatatt gctgcgcgag tcggcgaggg ttttcaccag cgccggagtc agatcctgcc
     7921 cggtcagcgc cgggaagtta tagagcatca ccggcagcgt gacgctgtcg gccacctgct
     7981 cgaaatagcg gatcaggttc gcttccgaca ctttccagta gtaggggttg atcaccacga
     8041 tgccgtccgc gcccgcctgc tgcgcgtgct ggctgagttc gatggtttcc cgggcgttgg
     8101 tgccgccggt gccgatcagc accggcacgc gacgatcgac atgatcgata gcaaagcggg
     8161 caatggcttt acgctcttcg gcgccgagct gggagaactc gccaccgctg cccaggaaga
     8221 acaggccgtc aacgcctgct ttgatcagat cgtcgatcag cgcggcggtg cccggcttat
     8281 cgagctggcc gtcggcggta aaaatggtgg agacaggggg aatgattccc gtgaacaacg
     8341 cggactgcgg catgagatct ccttgctgaa tcattttgtt ctacattata gaacagcgtt
     8401 cgtttattta acgatcatac tatgacgcag aaaaaggaga ggtaaatggg aagggaagag
     8461 ggagtgcggg aaatttaagc tggatcacat attgcccctc accccggccc tctccccaaa
     8521 ggggcgaggg ggaaaagatg gctcggtgca gtcccctctc cccctttggg gtgtacggtc
     8581 cggggacata gtgaacactt gttcggggac atggtagaca cttacaacta aggcataaga
     8641 acccgtttat ggagtcgctt atgccctggg atgcgagaga taccatgtca ttacgtactg
     8701 agtttgtttt gttcgcctcg caggacgggg cgaacatccg ttccctctgc cgtcgcttcg
     8761 gcatttcacc tgccaccggc tacaagtggc tccagcgctg ggctcaggaa ggtgccgccg
     8821 gtcttcaaga ccgcccgcgc attccgcacc attccccgaa ccgctcatct gacgacatca
     8881 cggccctgct gcgtatggcc catgaccgtc atgaacgctg gggagcccgc aagattaagc
     8941 gctggctcga ggaccagggg cacaccatgc ccgccttcag caccgtccat aacctgatgg
     9001 cccgccatgg cctgctgccg ggcgcttcac cgggcattcc cgccacgggc cggttcgaac
     9061 acgacgcgcc gaaccgcctc tggcagatgg attttaaggg ccactttcct tttggcggtg
     9121 gacgctgcca tccgctcacc ctgctggacg accactcccg tttttccctg tgcctggcgc
     9181 actgtaccga tgaacggcgc gagaccgtgc agcagcagct ggtcagcgtg tttgagcgtt
     9241 acggcctgcc ggaccggatg accatggata acggctcacc gtggggcgac accaccggca
     9301 cctggacggc gctggagctg tggctgatgc gcctgggtat ccgggtgggg cactcccggc
     9361 cttatcatcc gcagacgcag gggaagctgg agcgttttca ccgcagcctg aaggcggaag
     9421 tgctgcaggg aaaatggttc gcagacagcg gtgaactgca gcgcgccttc gaccactggc
     9481 ggacggtcta taaccttgaa cgcccgcacg aggcgctgga tatggcggta ccgggctcgc
     9541 ggtatcagcc gtcagcgcgg cagtacagcg gcaacacaac gcccccggaa tacgatgaag
     9601 gggtgatggt caggaaagtg gatatcagcg gaaagctgag cgtgaaaggg gtaagtctga
     9661 gcgcaggcaa ggcgttcagg ggagaacggg tcgggctgaa ggagatgcag gaagacggca
     9721 gctacgaggt gtggtggtac agcacgaaag tgggggtgat cgacctgaag aaaaagtcga
     9781 tcaccatggg taaaggatgt taaaaagtgt tcaccatgtc cccgaccacc tgattgtcgg
     9841 cggcaggcaa atattctcat tcgccgaaca tggtcttctt taacaggaat aaaattatga
     9901 ataaagactc tactcaaact tgggggctta aacgagatat cacaccgtgt ttcggagcac
     9961 gactggtgca ggaaggtcat cgtctgcatt ttctggctga ccgggctgga ttcaccggct
    10021 cattcagtga ggtgcagact ctacaactgg atgaagcctt cccgcatttt gtcgcacatc
    10081 tggagctgat gctgctctcc tgtgagctga atcccaggta cgcgcactgt gtcacactgt
    10141 accgtaacgg gctgactggt gaagccgata cgctgggcag tcatgggtat gtatatatcg
    10201 caattttgaa tcgccacggg tttaacagac acctccgagt catttaaaat ggcttaaaga
    10261 gaggtgccca tgagcggtaa gcgttatccc gaagagttta aaactgaagc agtcaaacag
    10321 gttgttgatc tcggttattc tgttgccagc gttgcaacac gtctcgatat caccacccac
    10381 agcctttatg cctggataaa gaagtacggt ccggattctt ccactaataa agaacagtca
    10441 gatgctcagg ccgagatccg ccgtctccag aaagagctga aacgggttac cgacgaacgg
    10501 gacatattaa aaaaagc
//
LOCUS       DAJSKJ010000132        10452 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000132 DAJSKJ010000000
VERSION     DAJSKJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.133"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            112..2490
                     /gene="ppsA"
                     /locus_tag="ON345_002913"
     CDS             112..2490
                     /gene="ppsA"
                     /locus_tag="ON345_002913"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR3072944.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYISDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2547..4193)
                     /gene="fadK"
                     /locus_tag="ON345_002914"
     CDS             complement(2547..4193)
                     /gene="fadK"
                     /locus_tag="ON345_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR3072945.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4250..4543)
                     /locus_tag="ON345_002915"
     CDS             complement(4250..4543)
                     /locus_tag="ON345_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR3072946.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDTGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4540..5829)
                     /locus_tag="ON345_002916"
     CDS             complement(4540..5829)
                     /locus_tag="ON345_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3072947.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVIARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLDDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5885..6823)
                     /locus_tag="ON345_002917"
     CDS             complement(5885..6823)
                     /locus_tag="ON345_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR3072948.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLMAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6843..7607)
                     /locus_tag="ON345_002918"
                     /pseudo
     CDS             complement(6843..7607)
                     /locus_tag="ON345_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            7923..8834
                     /locus_tag="ON345_002919"
     CDS             7923..8834
                     /locus_tag="ON345_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3072949.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSVFPQPVSLVG"
     gene            complement(8879..10030)
                     /locus_tag="ON345_002920"
     CDS             complement(8879..10030)
                     /locus_tag="ON345_002920"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR3072950.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(10044..>10452)
                     /locus_tag="ON345_002921"
     CDS             complement(10044..>10452)
                     /locus_tag="ON345_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HCR3072951.1"
                     /translation="MGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRV
                     KKFIQELPEITFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDK
                     MDFTPVISPELKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT         2510 a         2672 c         2639 g         2631 t
ORIGIN      
        1 aatatatttg cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc aaagataaat
       61 gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgatc gatgtccaac
      121 aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga tgtagacagg
      181 gttgggggca aaaatgcctc cctgggtgaa atgattacta acctttccgg aatgggtgtt
      241 tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct ggaccaaagc
      301 ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga tgttacccag
      361 cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt ccagcctgag
      421 ctggaaaatg ccatccgcga agcttatgca cagctttccg ccgatgacga aaacgcctct
      481 tttgcggtgc gctcctccgc tactgcagaa gatatgccgg acgcttcctt tgccggtcag
      541 caggaaacct tcctcaacgt tcagggtttt gacgccgttc tcgtggcagt gaaacatgta
      601 tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac
      661 cgtggtgtgg cgctctccgc cggtgttcaa cggatggtgc gctctgacct cgcatcttct
      721 ggcgtgatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt tatcacttcc
      781 gcatggggcc ttggtgaaat ggtcgtgcag ggcgcggtta acccggatga gttttacgtc
      841 cataaaccga cacttgcagc gaatcgcccg gctatcgtgc gtcgcaccat ggggtcgaaa
      901 aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa aatcgaagac
      961 gtaccgcagg aacagcgtga catcttctcg ctgaccaacg aagaagtgca ggaactggca
     1021 aaacaggccg tacaaattga gaaacactac ggtcgcccga tggatattga gtgggcgaaa
     1081 gatggccaca ccggcaaact gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc
     1141 ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc cgaaggccgt
     1201 gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtca tccatgacat cagcgaaatg
     1261 aaccgcatcg aacctggcga cgtgctggtt actgacatga ccgacccgga ctgggaaccg
     1321 atcatgaaga aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg tcacgcggcg
     1381 atcatcgctc gtgaactggg cattccggcg gtagtgggct gtggagatgc aacagaacgg
     1441 atgaaagacg gtgagaacgt cactgtttct tgtgccgaag gtgataccgg ttacgtctat
     1501 gcggagttgc tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc ggatctgccg
     1561 ttgaaagtga tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc ctgcctgccg
     1621 aacgaaggcg tgggccttgc gcgtctggaa tttatcatca accgtatgat tggcgtccac
     1681 ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag
     1741 atgatgaaag gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc
     1801 gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc tgattttaaa
     1861 tcgaacgaat atgccaacct ggtcggtggt gagcgttacg agccagatga agagaacccg
     1921 atgctcggct tccgtggcgc gggacgctat atttccgaca gcttccgcga ctgtttcgca
     1981 ctggagtgcg aagcagtgaa acgtgtgcgc aacgacatgg ggctgaccaa cgttgagatc
     2041 atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg tggttgagga actggcgcgt
     2101 caggggctga aacgtggtga gaacgggctg aaaatcatca tgatgtgtga aatcccgtcc
     2161 aacgccttgc tggccgagca gttcctcgaa tatttcgacg gcttctcaat tggctcaaac
     2221 gacatgacgc agctagcgct cggtctggat cgtgactccg gcgtggtgtc tgaattgttc
     2281 gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg cgattcgtgc cgcgaagaaa
     2341 cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg accacgaaga ctttgccgca
     2401 tggttgatgg aagaggggat cgatagcctg tctctgaatc cggacaccgt ggtgcaaacc
     2461 tggttaagcc tggctgaact gaagaaataa aataaatacc cggcggcgtt ttgtcgccgg
     2521 gtttatgtga tccccgaaga tgaaacttat tcaatctctt cacagacatc ctgagttaat
     2581 cgccgcataa tatctttcct taacaaaaac ttttgtattt tacctgaggc agtgcgcggt
     2641 agtttttcga ttaccacgat atgttcagga tatttatatt ttgcgacccg tttacggcta
     2701 aaaaaagcca ccacctcttc cagcgataat gaatgatgcg gcgctttcag cacgacataa
     2761 gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa ccacacaggc atcgtgaatt
     2821 ttaggatgct gcaataaaat atcttccact tcacggctgc taatattttc gccgccgcgg
     2881 acaataatat cttttttgcg tccggtaatt tttatatagc cagcctcatc catacggcag
     2941 agatcgccgc tgtaatacca gccttcttca tccagcgcac gggcggttaa ttcaggttca
     3001 tcaaaatatc ccataaacac attggggcca cgcgaggctt cttcaccttc gcaacctggc
     3061 ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc agcgtaacca
     3121 tcggtatgca taaagcgcga caaaggatca tcgagattca ccaccgcatg cggcgaactt
     3181 tctgtggaac cataaacact taataattta atgccgcgct gctggcattc acgcgccact
     3241 tttttgggga ttgtggtacc gccgcaaaga aagaagcgca gcgctgaaag gtctgcgggt
     3301 tgtttctcta gtaaattcaa aagatcatag acaaacggcg ttgcgccgag catacaggtg
     3361 caacgctgct gctcaagcag cgcgagacac gcatcaggag tgaaaatatc taacaacacg
     3421 ctgcgagcac caatcaaaaa tggtgctgtt acgccatgca gaaagcccgt tgcgtggccg
     3481 agtggcgcag gcatcataaa gacatcctgc caggtcagat tcagccgcgc gcaataagcc
     3541 cgctcactgg cgagaatatt gttatgcgtt agcatcacgc cctttggcag accctcggtt
     3601 ccggaggtaa acagcaccgc agctaattca tcgccgtggg tcgttatcgc cgtggtcagt
     3661 ggggtattgt cggcgataat ctgacttaat gagagggaag aggtggcggg agccagtttg
     3721 tccacgccga caatttgttg tagttgtgga agctgatttt gcagcggcag gattaaatct
     3781 accggacgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg acacttattg
     3841 agcacccata ccagttctgc ttcccgccag gaaggcaaca gcggtacgga aaccgcaccg
     3901 attttcaggc atgcaagata gataacagta aattcacacc agccaggcag ttgaaatgcg
     3961 atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca gctcgcggcg
     4021 tgatcgagcg cgctataggt gtacgatgct ccatgattat cgaccacggc aattttgtct
     4081 ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc ccatagccct
     4141 tgctgacgat acgccgcacg acgttgttcg ttaaacgtta atgtcacttt cattttggac
     4201 tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg gatgcatgat tagccgtagc
     4261 gaaaatcaat accgaaggtg cctgcgggat attgccactg ttcgagaata gtgttaccgc
     4321 acagcacccg acaggtgccg cactccagac aaccggcgga atcaaaatga atgtttcctg
     4381 tgtcatcctg cttataaagt ccggcagggc aggctttcat taatttacgg aattcattga
     4441 tatcgggatt tgccgccaga atgatatgcg ggtggccctc atcaacatgg aatttattga
     4501 cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg ttgctccctt
     4561 aatgccatct ttcagcaagt tgatcagccc aattttcttc gcgtgtccca tgatcatttt
     4621 gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc tcgttcatga tgtcggcgac
     4681 cattcgtggg tattggctaa acaggcgcgg gttttccatc agcgccggga tcttgcggaa
     4741 atgctgcata tcgcgcatga cacagctttg ttccagctca cgtttgtatt gcgccagact
     4801 gctggcggag aaatcctcgc gttctttggc ggcgatcacc gttgtggcgg cagcctgagc
     4861 cgatgcaatg gctaaatcca tgccacgaac tgtaaaaccc aaattcaggc agaagcctgc
     4921 ggcgtcacca acgatcatca cgccatcgtt aaccagctgc ggcaccattg ccagaccgcc
     4981 ttctggcacc atatgcgcgg aatattcaag cagtttgccg ccgctaatta gtgggcgaat
     5041 ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg cttttttgcg catgggcgat
     5101 atcatccaat ccacaaacca gccccaacga tacggaatcg ttgttggtat agaggaatcc
     5161 gccacccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac cttcctcgcc
     5221 cgtaatatta aagcgatcgt tgatctgttc tggcgtgagg ccaataacct ctttaacacc
     5281 aacagcgtaa tgatgcggat cggaagcggg aaccattccc agcgagcggc caagcatcga
     5341 gttaacgcca tcagccagaa tcaccacatt cgcttcgaga atatcatccc cagcctgcac
     5401 gccagttacc ttgtttcctt cacgaaccag cgcatcgacg cgaacgcctg ggataaactg
     5461 cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc agacgattac gcaatacggt
     5521 ataagatgcg tgttgcggaa catctggctg ctcgcggtga aaatcgaggg taacggcgct
     5581 ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg gcgctgatgc
     5641 tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa agacgcccgc cggtcatgtt
     5701 tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg ctcgtgctat
     5761 gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc acaatggcat caaatttgtc
     5821 atccgacatg cgttatgctc ccttcccgct gcgtttacag cgggagatgt ggttgaaagg
     5881 ggaattagcg gctcaactgg ctaatcaggg cagggacgac tttgtagata tcgcccacca
     5941 gaccgtagtc ggcatagttg aagattggcg catttttatc tttattgatg gcgacaatca
     6001 cttttgcgcc gttgccgcca accatatgtt ggatctgccc ggagatcccc agcgtcaggt
     6061 agagatcaga tttcagcaac acgccggaga caccgatata acgttcacgc tccatccagt
     6121 tctcgccttc ggcaattgga cgtgaacagc cgacttcagc attcagcacc gccgccagtt
     6181 cgtggaccat tttcaggtca tcctgcgccg caagaccacg accgacgcca accacacgtt
     6241 ttgctttgct caggtccacg ctgcttgcgg ctttcgcacg gcgttcgcga cagagaattt
     6301 catgacgcgg agcaacataa ggtaccgttt ctgtcgggca ctgatgagag gtatcactgg
     6361 tgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc cagcgagctg ttgatctttt
     6421 cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga ccatcgataa
     6481 tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag ttgcacactt aaccgtgctg
     6541 ccagagcttt accacgtttc gtcgcggcca ttagcaacat agcggggtgt ttatctttca
     6601 gcagagcagc aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg ttttgctcaa
     6661 gaacataaat acattttgga ccataaggca taactgcctg cgcctggtcg gtattttgta
     6721 caatggcata cacctgttgg ccccattgct gagcgccgcc aaacagttca gcataacgtt
     6781 caggattatc gctaaagacc cagacgctgt ttaattgact catcgtttct tctccgtagg
     6841 cttcagttca gggctttctt cagatgttcc gccagctcgg caatggcctc cggcgaatcg
     6901 ttatcgagaa tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc ggtcagttca
     6961 gcaagtggtg cgctctggct ccagccaata tcacttgcct gccactgatt taccggtttt
     7021 ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg gcacgttaat attggaggtg
     7081 acgcagagca cggctggaac agagagttca ataacttcaa cgttatcttc aagcgtgcgt
     7141 tcaatcacca gtgtattgcc ctgacgctga atagcactca ctgcattaat caccggaagt
     7201 tgcagaattt ctccgaccag caagccaacc tgctgggcat aaaggtcgcc ggaaccttca
     7261 ccaaagatca gtaaatcgaa gccgatcttt tcaactgctg ccgccagcgc ttttgcggta
     7321 tcgagaggca gtgcatgttc aagttgcgca tcctgcacca attacaggct gtgcggcccg
     7381 cgggataaca cgtctttgcg cactttcgag ttctgcaaca atgagccgcc aacggtcagc
     7441 gcggctatct catcgtcatc tgttgcaagc tggcttgcag cttcaatggc attgagatcg
     7501 aactggctga ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg agtgacaaca
     7561 atgtcctgtt cttcaggtac cagcttaaag caggttatta ttttcatgat ttctcctgtg
     7621 agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata
     7681 gcggtgcggg gttttcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa
     7741 tgcgatcgct aaaacaaaag tgcaatttgc agcaatatag ttattccatg accgtacaat
     7801 cttgcattca gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa
     7861 tacacgccgt attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg
     7921 tcatgtatca acgctgtttt gataacgcca gtgaaacgct gtttgtcgcc ggtaaaacgc
     7981 cacggctttc acgttttgca tttagcgatg atccaaaatg ggagtccgga catcacgttc
     8041 atgacaatga aaccgagttg atttacgtca agaaaggggt agcaaggttt accatcgatt
     8101 cttcgttata tgtcgcgcac gccgatgaca ttgtggtgat agaacgtggc aggctgcatg
     8161 cggtggcctc tgacgttaac gatccagcaa cgacgtgtac ctgtgcgctg tacggctttc
     8221 agtttcaggg ggctgaggaa aatcagctac tgcaaccgca ttcttgcccg gtaattgccg
     8281 cggggcaggg aaaagaagtc ataaaaacct tatttaatga gctaagtgtg attttgccgc
     8341 aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt tgcctataca ttagcaattc
     8401 tttactacga aaactttaaa aatgcttatc gttcggagca ggggtatatt aaaaaagatg
     8461 ttctgataaa agatattctt ttttatctga ataataatta tcgcgaaaaa atcactttag
     8521 aacagttatc gaaaaaattt cgtgccagcg tcagttatat ttgccatgaa tttaccaaag
     8581 agtatcgtat ttcccctatt aactatgtta ttcaacggcg tatgacggaa gcaaaatggt
     8641 cactcactaa tactgaatta tcacaggcag agatctcctg gcgcgtgggt tatgaaaatg
     8701 tcgatcactt tgccaaactg tttttgcgcc atgtcggttg ttcgcccagc gattaccgta
     8761 ggcaatttaa aaattgtttt gcggaacaag aaatcctatc tgtatttcct caaccggtaa
     8821 gtcttgttgg ataaatctgg cggcgcagtt tgttaagaag agctgcgccg cctgcagatt
     8881 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
     8941 taccgccgcc gatacgttca caacggacat cacgccagaa gcgagagacg cgcgcctcat
     9001 cggtatagcc cagaccgccc atgatttgaa tcgcatcatc aatgacttcc attgcggtac
     9061 gtgcacagta cagttttgcc agcgccgcgc tggtgcgcag tgattgatgc tgatcggctt
     9121 gccatgccac tttcagcacc atgttgcgca tgttgtcgat cttaatcgcc atcagcgcca
     9181 gtttttcctg gatcatctgg ttatgcccaa tgggtttacc aaaagcgata cgctggttgg
     9241 catagcgagc ggcatcttca aaggcacact cggcaaagcc ggtgctgcgc gcggcgttga
     9301 tcaggcgctc catctcaaag ttgtacatca cattgaggaa acccattcct tcttcgccca
     9361 ccatgtcgct ctcttcaact tccacgttgt cgagatagac ttcgcaggtg ctgagcatat
     9421 gccagccgat tttatgcagc gggttaatct taatgccggg cttactggag tcgacccacc
     9481 acagggtgaa ggcttttttg ggatctttcg gttgcggatc gcgcgccaac accagcatat
     9541 acgggtactc tttcgcaccg gtaataaagg ttttctgtcc gttgatgtaa accttgccgt
     9601 ttttacgcgt gtaagtggta gtggcactgt tgttgtctga gcctgcgcct ggctccgtca
     9661 acgccagggc ataggcggga tcacccgttt ccagggtgct ctctgccgtt ttacgtagct
     9721 gctctgcaga accgaaacgg cgcatactgt gaatacattg accgttggta atcaaaaatg
     9781 ccggagcacc gcattttgac acttccatca gcgccagcat ctgggtgacg taatccgcag
     9841 ggatgccacc gaactcttcc ggcacgccaa gcatagaaat gccgttatcc gccagcgccc
     9901 gcataaactc acgcggatat gtcccgtttt gatcgcaggt gcggaaatac tcttccgcaa
     9961 agttagtcgt aatcagttcg cgaatactgg ccagcagcag ttcttgttct tcagttaaag
    10021 aaaaatccat cgtgatactc ctgttaatga gccgcttcag gcaggacaaa acccatcgcc
    10081 gcatcgataa ataatctttc gtccatcagt ttgagttctg gcgaaatcac tggggtgaaa
    10141 tccattttgt cgagaatatc tttttgtaaa tcgacgccag gggcgatttc gattaaatgc
    10201 aggccgtctt ctttcagcgt gaatacagca cgttcggtga tgtaacgaac atccagttcc
    10261 cgttcgagcg cgatttttcc gctaaaagta atttccggta gttcctgaat aaatttcttc
    10321 acccgtcctt cctggacgat atttaatttg ccatcggcaa tttctgtttt taaactgccc
    10381 gcggttaacg tgccgcagaa aataattttc ttcgaggtgg cactgatatc aataaatcca
    10441 ccggtaccca tg
//
LOCUS       DAJSKJ010000133        10400 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000133 DAJSKJ010000000
VERSION     DAJSKJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.134"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1324
                     /gene="dcuC"
                     /locus_tag="ON345_002922"
     CDS             <1..1324
                     /gene="dcuC"
                     /locus_tag="ON345_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR3072952.1"
                     /translation="YLILKNYKPQVVLAAAGIFLMMCGVWLGFGGVLDPAKSSGYLIV
                     DIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYIIL
                     SATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILE
                     TNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDNKDKNINHEQAEQ
                     KALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVVMLLSLTVTMFVEFFR
                     KHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLG
                     GIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLA
                     RAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(1367..1864)
                     /gene="sspB"
                     /locus_tag="ON345_002923"
     CDS             complement(1367..1864)
                     /gene="sspB"
                     /locus_tag="ON345_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR3072953.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1870..2508)
                     /gene="sspA"
                     /locus_tag="ON345_002924"
     CDS             complement(1870..2508)
                     /gene="sspA"
                     /locus_tag="ON345_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR3072954.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2903..3295)
                     /gene="rpsI"
                     /locus_tag="ON345_002925"
     CDS             complement(2903..3295)
                     /gene="rpsI"
                     /locus_tag="ON345_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3072955.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3311..3739)
                     /gene="rplM"
                     /locus_tag="ON345_002926"
     CDS             complement(3311..3739)
                     /gene="rplM"
                     /locus_tag="ON345_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR3072956.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(3958..5085)
                     /gene="zapE"
                     /locus_tag="ON345_002927"
     CDS             complement(3958..5085)
                     /gene="zapE"
                     /locus_tag="ON345_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR3072957.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5279..5677
                     /locus_tag="ON345_002928"
     CDS             5279..5677
                     /locus_tag="ON345_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR3072958.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LNEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5831..7198
                     /gene="degQ"
                     /locus_tag="ON345_002929"
     CDS             5831..7198
                     /gene="degQ"
                     /locus_tag="ON345_002929"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR3072959.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7288..8355
                     /gene="degS"
                     /locus_tag="ON345_002930"
     CDS             7288..8355
                     /gene="degS"
                     /locus_tag="ON345_002930"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR3072960.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8418..9356)
                     /gene="mdh"
                     /locus_tag="ON345_002931"
     CDS             complement(8418..9356)
                     /gene="mdh"
                     /locus_tag="ON345_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR3072961.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9791..10261
                     /gene="argR"
                     /locus_tag="ON345_002932"
     CDS             9791..10261
                     /gene="argR"
                     /locus_tag="ON345_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR3072962.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2541 a         2652 c         2610 g         2597 t
ORIGIN      
        1 ctatttgatc ctgaaaaact acaaacctca ggtggtgctg gctgccgcag gtatcttcct
       61 gatgatgtgc ggtgtctggt tagggttcgg tggtgtactc gatcccgcca aaagcagcgg
      121 ctacttgatc gtcgatattt ataatgaaat cctgcgcatg ctgtccaacc gcattgccgg
      181 attggggctg tcgattatgg cggtgggcgg ttatgcccgc tacatggagc gcataggggc
      241 cagtcgcgcg atggtgagct tgttaagccg cccgttaaaa ctcattcgct cgccgtatat
      301 tattctgtcg gcaacttacg tcatcggtca aatcatggcg cagtttatta ccagcgcctc
      361 cggtctgggt atgttgctga tggtcacctt atttccgaca ctggtgagtc tgggagtaag
      421 tcgtctctct gcggtggcgg ttatcgcaac cacaatgtcc attgagtggg gaattctgga
      481 aacgaactcc atttttgcag cccaggtcgc gggaatgaaa attgccactt acttcttcca
      541 ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg gcgatctccc actttttcgt
      601 gcaacgcgct tttgacaata aagataaaaa tatcaatcac gaacaagcag agcaaaaagc
      661 tctcgataat gtcccgccgc tctattacgc cattttacct gtgatgccgt taatcttgat
      721 gctcggctcg ctgttcctcg cccacatcgg gctgatgcag tcagaactgc atctggtggt
      781 ggtgatgtta ctgagtttga cggtgacgat gtttgttgag ttcttccgca agcataactt
      841 gcgcgaaaca atggacgatg tgcaggcgtt ttttgacggc atgggtacgc agtttgccaa
      901 cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa ggcttaacga cgattggcac
      961 tgttgatgcg gttatcaggg gtgcggagca ttctggtctg ggcggtattg gcgtgatgat
     1021 tattatggcg ctagtcattg ccatttgtgc cattgtgatg ggctctggca atgcgccgtt
     1081 tatgtcattt gccagtctta ttccgaatat cgcagccgga ctacatgtac cagcggttgt
     1141 aatgattatg ccgatgcatt ttgccacgac gctagcgcgc gcggtttcgc cgattactgc
     1201 ggtggtggtc gtaacgtcag gaattgcagg cgtttcgcct tttgcggtgg tgaagcgaac
     1261 agcgatcccc atggcagtcg gtttcgtggt gaatatgatt gccacaatca cgctatttta
     1321 ttaagccatt aaaagacaaa acaggccacc agggcctgtt tttgtattac ttcacaacgc
     1381 gtaatgccgg tcgaccaccg cgtggtggct gcggaggttc atcgtcagga tgagtgtcat
     1441 catcgtgatc tggcttgtcg ccatcaataa ccgacataac ggtttcgttg tctgccgatg
     1501 cctcttcatc attcatgatg ctggtatctt catcgtaggc agcttcaggc tcaaacattg
     1561 tgcctgcgcc attttcacgg gcgtagatag ccagcacggc agccagcggc acagaaacct
     1621 gacgcggaat gccgccaaag cgcgcgttaa agcgcacctc atcattcgcc agttccagat
     1681 tgccgacagc acgcggcgca atgttgagta cgatttgccc gtcacgcgca tattccatag
     1741 gaacctgcac gccagggagc gtcacatcca ccaccaggtg cggcgtgagc tggttatcca
     1801 gcaaccactc atagaatgca cgcagcagat agggacgacg tggtgttagc tgtgacaaat
     1861 ccatacagat taactccggc ccagacgcat ttcacgttct gcttcagtta aagaagcaag
     1921 gaaagagtca cgctcaaaga cgcgggtcat atagcctttc agctctttcg cacccgggcc
     1981 gctgaactcg atgcccagtt gcggcagacg ccacagcagc ggagcaagat agcaatcgac
     2041 caggctgaac tcatcgctca ggaagtacgg cttctgaccg aagaccggcg caatcgccag
     2101 cagttcttcg cgcagttgct tacgtgcggc atctgcttca gaagctgaac cgttgatgat
     2161 ggtgttcatc agcgtgtacc agtctttttc gatgcgatgc atgtacagac ggctttcacc
     2221 gcgagctacc gggtaaacag gcatcagtgg cggatgcggg aaacgctcat ccagatattc
     2281 cataatgatg cgagattccc acagggtcag ctcacgatcc accagggtcg gaacgctctg
     2341 attcgggttg aggtcaatca gatcctgagg cggattgtcc ttttccacgt gttcgatctc
     2401 gaaacttaca cctttctcag ccagcacaat gcggacctga tggctataga tgtcagtagg
     2461 accggaaaac agcgtcatta ccgaacgttt gttggcagcg acagccatga aaacctccag
     2521 gtatagtcag aatttttact gctaccagcc accaggtggc cagtcagaag ttgtgttacc
     2581 cagtaaggaa cgactctctt tgttcgaaaa tcaaacaaaa aatgagcaat acccgacatt
     2641 tgggcagaaa attggatgat agtttaccag attttgcgac ctttgtggtg agtcgatgcc
     2701 ggaaatgggg aaaaagagat gcgctttagt ctgaaatagt tgacttagtc ccttattggc
     2761 gatgtggttt ttgttttgcc tgtctgtcag acggtagcaa aaagcaactt tccatttttt
     2821 acgctgattc agaatttagc cataaaaaaa cccgccgaag cgggtttttt cgcaaattgt
     2881 tttctgccgg agcagaagcc aattaacgtt tggagaactg cggacgacga cgtgctttac
     2941 gcagaccgac tttcttacgt tcaacctgac gagcgtcacg agtaacgaag ccagctttac
     3001 gcagttcaga acgcagggac tcgtcgtatt ccatcagagc gcgggtgata ccgtgacgga
     3061 tcgcaccagc ctgaccagag ataccaccac ctttaacggt gatgtacagg tccagtttct
     3121 caaccatgtc gaccagttcc agcggctgac gaactaccat gcgggcagtt tcacgaccga
     3181 agtactgttc cagagaacgt tggttgatta cgattttacc gttgcccggt ttgatgaaaa
     3241 cgcgagctgc ggaacttttg cggcgaccag tgccgtagta ttgattttca gccattgcct
     3301 ataatcccga ttagatgtca agaacttgcg gttgctgtgc cgcgtggttg tgctcgttac
     3361 ccgcgtaaac tttcagttta cggaacatag cacgacccag cgggcctttt ggcaacatgc
     3421 ctttaaccgc gatttcaatc acacgctcag gacggcgagc aatcatctct tcaaaggtcg
     3481 cttgtttgat accaccgatg tggccggtgt ggtgatagta cactttgtca gtacgcttgt
     3541 tgccggttac agcaactttg tcagcgttca gaacgatgat gtaatcaccg gtatctacgt
     3601 gcggagtata ttccgctttg tgcttaccgc gcaggcgacg agccagttca gtagccagac
     3661 ggcccagagt tttaccggtc gcgtcaacaa cataccagtc gcgttttacg gtttctggtt
     3721 tagctgtaaa agttttcatt aaaagcttac ccaataaata gttacacgtt ggtgaacacc
     3781 caaacgtctt caattgttga ggttcacacg acaaagtccg gcaaacctac cccttcgaat
     3841 agcctatgcc agcacacaaa aagttttggg aaaaaaactt tcttgtaacg tggggtcgca
     3901 ggattataga gaagtcgggg tcaaagatcg accccttttt gtgatttgtg gcaggtttta
     3961 acccgccaaa tgctcgcgct tcagatactc ttcgctttgc atctcttgca gacgtgacag
     4021 gcaacgctgg aactcaaact tcagccgctc accctgataa atttcataca gcggcacttc
     4081 tgcactcacc actaatttga catggcgctc gtaaaactca tccaccagcg caataaagcg
     4141 ccgcgcttcg ctctccatca accgcgtcat gactggcaca tcaaacaaca ttactgtgtg
     4201 aaacaggcgt gagagcgcaa tatagtcatg ctgactgcgg gcgtcgacgc acagcgtagt
     4261 aaaagagacc gccagcgtct ggttctcgac gcccattgtc gccaatggcc gatggttgat
     4321 ttctaacgtc ggtgaatttt ctcgtttcgc ccccgccagc gccaaccata gtttatccat
     4381 ttgcgcccgg gtttcatcgt tgagtggcga aagccacaga tgcgcctgag tgagtgtacg
     4441 cagacgataa tcaacaccag cgtccacgtt cattacatca caatgctgtt taatggcatc
     4501 gattgcaggc agaaaacgcg cacgttgcag gccatttcga taaagttcat ccggcggaat
     4561 atttgacgtc gctaccaggg taataccgcg ggcgaacagg gctttcatca gaccgccaag
     4621 tagcatggcg tcggtaatat cagaaacaaa aaattcgtca aaacagagca cgtcagtttc
     4681 ggctttaaag cgatcggcaa taatttccag cggatcggtc tgcccctgta aggcagttag
     4741 ctcttcgtgc acccgcagca taaaacggtg aaagtgcagg cgctgtttcc gctctcccgg
     4801 caggctttga tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat
     4861 atataagcca cgcactggcg tatgctttgt atcttcgcgt ttaccccaca gcttaccgac
     4921 ccgcgccatt agcccactcg tcctgggggc tggtggcgtg ctattgatga gttcctgata
     4981 aataatttcc aggcggctga cggcctcttt ttgaacgtcg tcgggttgat ggctgccttc
     5041 attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct tattgttatt
     5101 ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac
     5161 gcgatatgca gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc
     5221 gcaggcatgg agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat
     5281 gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc attattggtg ctgtggccat
     5341 gcgttttggt aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa
     5401 taaagctgaa ctgaacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga
     5461 attactggat accatggcgc acgactatcg ccagctgtat cagcacatgg cgaaaagctc
     5521 cagcagcctg ctgccggaac tgtctgctga agcaaacccg ttccgtaatc gtctggcaga
     5581 atctgaagcc agcaacgatc aggcaccggt gcagatgcct cgcgactatt ctgaaggcgc
     5641 atccggcctg ctgcgtactg gcgcgaagcg cgactaattt atttttcggg cgcagccatt
     5701 gcgccctcct cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg
     5761 tctgaaaatt caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag
     5821 agcaggaata atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt
     5881 aactctctcg gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg ccgatcaggc
     5941 ccctctcccc agtctggctc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg
     6001 ggtggaagga acagccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg
     6061 tgatgattta ccggatcaac ctgcacaacc cttcgaaggt ttaggctccg gtgtcatcat
     6121 caacgccagt aaaggctatg tgctgaccaa caaccatgtg attaatcagg cacagaaaat
     6181 cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca
     6241 gagcgatatc gccctgttgc aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc
     6301 cgactccgat aaattgcgcg tcggtgattt tgccgtggcg gtcggtaacc catttggcct
     6361 tgggcaaacc gccacctctg gcattgtttc cgcattaggc cgcagtgggt tgaatcttga
     6421 aggtctggaa aactttatcc agacagatgc ttccattaac cgcggtaact ccggcggtgc
     6481 actattaaac cttaacggtg agttaattgg catcaacact gcaatccttg cgcctggcgg
     6541 cgggagcgtc gggattggat ttgccatccc cagtaatatg gcgcgaacac tggcgcagca
     6601 gcttatcgac tttggtgaaa tcaaacgcgg tttgttaggc atcaaaggca ccgagatgag
     6661 tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt ggcgcgtttg tcagcgaagt
     6721 gttgccaggt tctggctcgg caaaagcggg cgtcaaagcg ggcgatatta ttaccagcct
     6781 caacggcaaa ccgctgaata gctttgctga gttgcgctct cgtatcgcga ctacagagcc
     6841 gggcacgaaa gtgaagcttg gcctgctgcg taacggcaaa ccactggaag tagaagtgac
     6901 gcttgatacc agcacctctt cgtcggccag cgctgaaatg atcacgccag cgctggaagg
     6961 tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa ggtattaaga tcgatgaagt
     7021 tgtcaaagga agcccagctg ctcaggctgg cttgcaaaaa gacgatgtga tcattggcgt
     7081 caaccgcgat cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg caaaaccggc
     7141 catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc tatctgctga tgcgttaatg
     7201 tcgtaaaccg ggcatcaggc ttacgtgtga tgtccggtta actcgtggta tgctgctgcc
     7261 gttccctttt ttaatgacgc ctccatcatg tttgtgaagc tcttacgttc cgttgcgatt
     7321 ggattaattg tcggcgctat tctgctggtt gccatgcctt cgctgcgcag ccttaacccg
     7381 ctttccactc cgcaatttga cagtaccgat gagacgcctg ccagctataa tctggcggtt
     7441 cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg gtttgaacac caactctcac
     7501 aaccagcttg agatccgcac cctgggatcc ggtgtaatca tggatcaacg cggttatatc
     7561 atcaccaata aacacgtcat caacgacgcc gatcagatca tcgtcgcctt acaggatggt
     7621 cgtgtatttg aagcattgct ggtgggatct gactctctaa ccgatctggc ggtacttaaa
     7681 attaatgcca ctggcggttt acctaccatt ccgatcaatg cacgtcgcgt accgcacatt
     7741 ggcgacgtag tactggcgat cggtaacccg tacaacctcg ggcagaccat tacccagggg
     7801 attattagtg ccacgggtcg aatcggtctg aacccgaccg ggcggcaaaa cttcctacaa
     7861 accgatgctt ccattaacca cggtaactct ggcggcgcgc tggtgaactc gctgggcgaa
     7921 ctgatgggca ttaacacgct gtcgtttgat aagagtaacg atggcgaaac gccggaaggt
     7981 atcggctttg cgattccttt ccagttagca accaaaatta tggataagct gatccgcgat
     8041 gggcgcgtga tccgcggcta cattggcatt ggtggacgtg agatcgcacc actgcacgcg
     8101 cagggcggcg gtatagatca actgcaaggg atcgtggtta atgaagtgtc acctgacggc
     8161 ccggcggcga atgcgggtat tcaggtcaac gatctgatta tttcggtgga taacaaaccg
     8221 gccatctctg ctctggagac gatggatcag gtggcagaaa ttcgccctgg ttcggtgatc
     8281 ccggttgtag tgatgcgtga tgataagcag ttaacgctgc aggtcaccat tcaggaatat
     8341 ccggcaacca attaagtcga gtgctcaaaa caaaaaaccg gagtctgtgc tccggttttt
     8401 tattattcgc taatcaatta cttattaacg aactcttcgc ccagggcgat atctttcttg
     8461 agcgtatcca gcataccttc cagcgcgttc tgttcaaatg cgctcagggt accgatagat
     8521 ttacgctctt ccacgccgtt tttacccagc agcagcggtt gagagaagaa acgggcgtac
     8581 tgaccgtcgc cttcaacgta ggcacattcg acaacgcctt gttcgccctg cagtgcacga
     8641 accagagaca gaccaaaacg tgcagctgcc tggcccatag acagggttgc agacccgcca
     8701 ccggctttcg cttcaaccac ctcagtaccc gcgttctgga tacgtttggt cagatcagcc
     8761 acttcctgct cggtaaaact aacgccagga acctgtgaca gcagcggcag aatggtaaca
     8821 ccagagtgac cgccaataac cggcacttca acttcgcctg gctgtttgcc tttcagttcc
     8881 gcaacaaagg tgttggaacg aatgatatcc agcgtggtaa cgccgaacag tttgtttttg
     8941 tcataaacac cggctttttt cagcacttca gcagcaatcg caactgtggt gttaaccggg
     9001 ttagtgataa taccaatgca cgctttcggg caggttttcg caacttgctg taccaggttt
     9061 ttcacgatgc cggcgttaac gttaaacagg tcggaacgat ccatacccgg tttacgcgct
     9121 acacctgcag agataagaac gacatctgcg ccttccagcg ccggagtcgc atcttcacca
     9181 gaaaaacctt tgattttcac agcagtaggg atatggctca gatcgacagc cacaccggga
     9241 gtcactggag cgatatcata cagagagagt tctgaacctg aaggcagttg ggtttttaac
     9301 agtagtgcaa gcgcctggcc aataccgcca gcagcgccga ggactgcgac tttcatccta
     9361 aactccttat tatattgata aactaagata tgttgctccg ctgccgcgac cttaatccac
     9421 aaaattgccg tttacaatga ccacatctca agaatgtgta gtcacgcaag tttagcgttt
     9481 atgcatttaa ttgccgtaat caggaaccta acttacgtaa ttaacagtcg cttatgcatt
     9541 agcgcaacat tccaacaggt ggtgacaata taccctaccg ttcagccaaa acaacatcaa
     9601 tttgataaca attaatttac ttttaagcag aatttgcatg ccgtgacgca ggcatgtttc
     9661 tcaataacga aatttgataa aatcccgctc tttcataaca ttatttcagc cttcttcagg
     9721 gctgactgtt tgcataaaaa ttcatctgta tgcacaataa tgttgtatca accaccatat
     9781 cgggtgactt atgcgaagct cggctaagca agaagaacta gttaaagcat ttaaagcatt
     9841 acttaaagaa gagaaattta gctcccaggg cgaaatcgtc gccgcgttgc aggagcaagg
     9901 ctttgacaat attaatcagt ctaaagtctc gcggatgttg accaagtttg gtgctgtacg
     9961 tacacgcaat gccaaaatgg aaatggttta ctgcctgcca gctgaactgg gtgtaccaac
    10021 tacctccagt ccattgaaga atctggtgct ggatatcgac tacaacgatg cagttgtcgt
    10081 gattcatacc agccctggcg cggcgcagtt aattgctcgc ctgctggact cactgggcaa
    10141 agcagaaggt attctgggca ccatcgctgg cgatgacacc atctttacca cccctgctaa
    10201 cggtttcacc gtcaaagacc tgtacgaagc gattttagag ctgttcgacc aggagcttta
    10261 atctctgccc cgtcgcttct ggcggcgggg aaaatgttgc ttatccctct cattcccctt
    10321 ctttctcctg cgatacattt aacgaatagt gcgttttacc gcgacacatc attcacaaaa
    10381 tgaatacata cggtaaaaaa
//
LOCUS       DAJSKJ010000134        10399 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000134 DAJSKJ010000000
VERSION     DAJSKJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.135"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            143..718
                     /gene="ssuE"
                     /locus_tag="ON345_002933"
     CDS             143..718
                     /gene="ssuE"
                     /locus_tag="ON345_002933"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR3072963.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHNPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            711..1670
                     /gene="ssuA"
                     /locus_tag="ON345_002934"
     CDS             711..1670
                     /gene="ssuA"
                     /locus_tag="ON345_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR3072964.1"
                     /translation="MRNIIKLALAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEQPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYVDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1667..2812
                     /gene="ssuD"
                     /locus_tag="ON345_002935"
     CDS             1667..2812
                     /gene="ssuD"
                     /locus_tag="ON345_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR3072965.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2824..3615
                     /gene="ssuC"
                     /locus_tag="ON345_002936"
     CDS             2824..3615
                     /gene="ssuC"
                     /locus_tag="ON345_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR3072966.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3612..4379
                     /gene="ssuB"
                     /locus_tag="ON345_002937"
     CDS             3612..4379
                     /gene="ssuB"
                     /locus_tag="ON345_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR3072967.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4477..7089)
                     /gene="pepN"
                     /locus_tag="ON345_002938"
     CDS             complement(4477..7089)
                     /gene="pepN"
                     /locus_tag="ON345_002938"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR3072968.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7355..8557
                     /gene="pncB"
                     /locus_tag="ON345_002939"
     CDS             7355..8557
                     /gene="pncB"
                     /locus_tag="ON345_002939"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR3072969.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8726..10126
                     /gene="asnS"
                     /locus_tag="ON345_002940"
     CDS             8726..10126
                     /gene="asnS"
                     /locus_tag="ON345_002940"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR3072970.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCDRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2345 a         2672 c         2797 g         2585 t
ORIGIN      
        1 gatatataaa gccctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg
       61 gcattttctt gtctctccga cagactgacc gaattgttat caatgttaac aaaaaaagaa
      121 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
      181 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaattgcc tggatgtaga
      241 ggtttatcac tggaatctgc aaaacttcgc cccggaagat ctgctttacg ctcgtttcga
      301 tagtccggca ctcaagacct tcaccgaaca gctgcaacag gctgatggac tgatagtcgc
      361 tacacctgtg tataaagccg cctattccgg cgcgctgaaa accctgctcg acctgctgcc
      421 agagcgcgct ttgcaaggca aagtggtgct accgctggcg acgggcggta cggtggctca
      481 tctgctggcg gtcgattatg cccttaagcc agttttaagt gcgatgaaag cccaggagat
      541 cctgcacggc gtgtttgccg atgactcaca agttattgat taccatcaca acccccagtt
      601 cacgccaaat ctgcaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca
      661 ccgccgcgat gttcaggttc ctgaccttct gtctctgcga ggtaatgtcc atgcgtaaca
      721 tcattaaact ggcgctggcg ggattgctta gcgtctctac gattgcggtt gctgcagaat
      781 cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg gtattggcaa
      841 aaagccacca gttactggaa aaacgctatc cgcaatcaaa aatctcctgg gtggagttcc
      901 ccgcgggtcc gcaaatgttg gaagcgttaa acgttggcag tattgatctc ggcagtaccg
      961 gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtatat gtgggcgtcg
     1021 agcaaccgaa gcccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg
     1081 tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttca cacaaccttt
     1141 tactgcgtgc actgcgtcag gccggactta agtttactga catccagccc acttatctga
     1201 cgccagctga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     1261 atccctacta ctccgctgcg ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg
     1321 atctcaatca aactggatcg ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg
     1381 cttttattca gggcgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc
     1441 gcgagcaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct
     1501 cgtatgtgga tcatcgtcct cccaccacca tcaaaccggt gagcgctgaa gttgccgcct
     1561 tacagcagca aacggcagat ctgttttatg aaaaccgtct ggtgccgaaa aaagttgata
     1621 ttcgccagcg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     1681 gttctggttt ttaccgaccc atggtgacgg gcattatctt ggaacggaag aaggttcacg
     1741 cccggttgat cacggctatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggctatac
     1801 cggtgtgctg atcccaacgg ggcgatcgtg tgaagatgcg tggctggtgg cggcgtcgat
     1861 gatcccggtg acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc
     1921 taccgttgcc gctcgccagg ccgccacgct tgaccgtctc tcaaatggtc gtgcgttgtt
     1981 taatctggtc acaggcagcg atccacaaga gttggcaggc gacggcgtgt tccttgatca
     2041 tagcgagcgc tacgaagcct cggcggaatt tacccaggtc tggcggcgct tattgctggg
     2101 ggaaaccgtc aatttcaacg gtaaacatat tcatgtgcgc ggagcaaaac tgctcttccc
     2161 gccgattcaa cagccttatc ctccgcttta ctttggcgga tcgtcagatg tcgcccagga
     2221 gctggcggca gaacaagttg atctctacct cacctggggc gaaccgccgg aacttgttaa
     2281 agagaaaatc gaacaagtgc gggcgaaagc tgccgcgtat gggcgcaaaa ttcgtttcgg
     2341 tattcgtctg catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg
     2401 gttaatctcg catcttgatg atgagactat cgccaaagca caagccgcat tcgcccggac
     2461 ggattccgta ggacaacagc gaatggcggc gctacataac ggcaagcgcg acaatctgga
     2521 gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg gaacggcgct
     2581 ggtgggcgat ggtcctacgg tcgccgcgcg aatcaacgaa tacgccgcgc ttggcatcga
     2641 cagctttgtg ctttcgggct acccgcatct ggaagaagcg tatcgggttg gcgagttgct
     2701 gttcccacat ctggatgtcg ccattccgga aattccccag ccgcagccgc tgaatccgca
     2761 aggcgaagcg gtggcgaatg attttatccc ccgtaaagtc gcgcaaagct aaggaacacg
     2821 atgatggcaa cgccagtgaa gaaatggtta ttgcgcgttg ccccctggtt tttaccggtg
     2881 ggcatcgtgg cggtgtggca actggcctcc tcggttggct ggctgtcgac gcgtattttg
     2941 ccttcaccgg aaggagtggt gacggcgttc tggacgctct ccgccagcgg cgaactgtgg
     3001 cagcatctgg cgatcagttc ctggcgggcg ctgattggct tttcaattgg cggatcgctg
     3061 ggcctgattc tgggactgat tagcggactg tcgcgctggg gagaaaggct gctggatacc
     3121 tcaattcaga tgttgcgcaa cgtgccgcat ctggcgctga tcccgctggt gattttgtgg
     3181 tttggcattg atgaatccgc gaaaatcttt ctggtggcgc tcggtacgct gttccccatt
     3241 tatatcaaca cctggcatgg catccgtaat atcgatcgcg ggctggtgga gatggcgcgt
     3301 agctatggat tatccggcat accgctgttt atccatgtga tcctgcccga tgccctgccc
     3361 tcaattatgg tcggcgtgcg ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc
     3421 gaaaccattt ctgccaattc aggcattggt tatctggcga tgaatgcgcg ggagtttttg
     3481 caaacggacg tggtggtagt cgctattatt ctttacgccc tgctcggcaa acttgctgac
     3541 gtcagcgcgc agctgttaga gcgcctctgg ttgcgctgga acccggctta tcatttgaag
     3601 gaggccacgg tatgaatact gctcgtctga accagggcac accattgttg ctcaatgcag
     3661 taagcaaaca ttacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag
     3721 gtcagtttgt ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc
     3781 tggcaggtct ggaaacgcca accgcaggcg atgtgttagc gggcaccaca ccgctggctg
     3841 agattcagga agacacgcga atgatgtttc aggatgcgcg tctgctgccg tggaaatcgg
     3901 tgattgataa cgttgggtta ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt
     3961 tggctgcagt aggactggag aatcgcgccg gggaatggcc tgccgcactt tctggcgggc
     4021 agaaacagcg tgtggcgctg gcaagagcgt tgattcatcg accgggttta ttgttgcttg
     4081 atgaaccgct cggggcgctg gacgccttaa cgcgactcga gatgcaggat ttgattgtgt
     4141 cactttggca gcagcacggc tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg
     4201 tagcaatggc tgaccgggtg ttgttaattg aagagggaaa aattggtctg gatttgacag
     4261 tggatatccc ccgtccgcgt cgattagggt cggtgcgatt agcggagctg gaagcggaag
     4321 tattgcaacg ggtaatgcgg cgtgggcatt ctgaacaact gattcggcgt catggttaat
     4381 atgccggatg tggcgcacaa aaccagcatt ttcaacggac tagtaggcct gataagacgc
     4441 gtcaagcgtc gcatcaggca ttttggctca aaagtatcaa gccagtgctt tggtgatctt
     4501 ctcgtacaga tcgccagaga gattttccag ccctttcaac tgttccagcg ccgcgcgcat
     4561 tttctcctga cgtttggcat cataacgttt caggcgaatc agcggttcaa tcagacgtga
     4621 agctacctgc gggttacggc tgttgaggtc ggtaagcatt tccaccagga actggtaacc
     4681 gctgccatct tcggcatgga acgctgccgg gttgctgccc gcaaacgcac caatcaacga
     4741 acgaatacgg ttcgggttgc tcatggtaaa tgagcgatgc tgcaacagac cacgcaccgt
     4801 ctccagcaca ttcgccgccg ggctggtggc ttgcaggata aaccatttat ccatcaccag
     4861 accgtcctga tgccacttat cgtcgtactc ctgcatcagc gcgtcacggc aaggcagctg
     4921 tgcagcaacc gccgcagaaa gcgctgccag cgcatcggtc atattgtttg cttcatggta
     4981 ttgcttgctc accagcacgt cagccagatg cgtttcacca aaagcaagga agcgcaggca
     5041 ggcattacgc agagtgcgtt ttgcaatatc ttcatgctca acacggtact cgctctggta
     5101 attcgcgttg taaatagcca gcagctcatc cgccagttca gtcgccagag tacgagtgag
     5161 tgcttcgcgt acttcggcaa tagcaatcgg gtcgatgata tcgaacagtt cagccatttc
     5221 attgacagaa ggcagcgtca ggatttctgc cgccagcgcc ggatcaatct tctcatcgag
     5281 cagcaccgcg cggaaagcgt cagccacatg taccggcaga gacagcggct gcccttgctg
     5341 atgacgggcg acgttcagct tgatgtaggt tgccagcaaa ctttgcgccg catcccaacg
     5401 ggagaaatca ttacgcgcat gacgcatcag gaaggtcagt tgctgatcgc tccacttata
     5461 ttccagtttc actggcgcag agaattcgca cagcagcgca ggcaccggct ggaagtagac
     5521 attatcaaaa acaaaggtct gttccgcctg ggtgacgttc agcacggaat tcaccggatg
     5581 accgcctttc tgcaacggga tcactttgcc ttcgttgtca tacaattcga tggcaaatgg
     5641 aatatgcagc ggctgttttt ctgcctgatc cggcgtggct ggcgtgcgct ggctgatggt
     5701 cagggtgtac tgctcggttt ccggattgta gtcgtctttg acggtcacaa tcggcgtgcc
     5761 ggactggctg taccaacggc ggaaatggga gagatcgaca ttcgacgcat cttccatcgc
     5821 ctgcacaaag tcgtcgcagg ttgccgcgct gccatcatgg cgctcgaaat atagttgcat
     5881 ccctttctgg aagttttctt cgcccagcag ggtgtggatc atgcgaatca cttccgcgcc
     5941 cttctcgtaa acggtcaggg tgtagaagtt gttcatctca atgaccatat ccgggcggat
     6001 cgggtgcgcc atcgggctgg cgtcttccgc aaactgcaat ccgcgcatgg tgcgcacatt
     6061 attgatacgg tttaccgcgc gggaaccaag gtcagagctg aattcctgat cgcggaagac
     6121 ggttaaacct tctttcaggc tgagctggaa ccagtcgcgg caggtcactc ggttaccggt
     6181 ccagttatgg aaatattcat ggccgataac gcgttcaata tcgagataat ctttgtcggt
     6241 ggcggtgtcg gtgcgggcca acacatattt ggagttaaag atattcagac ccttattctc
     6301 cattgcgccc atattgaaga aatccaccgc gacgatcata tagatgtcga ggtcatactc
     6361 caggccaaag cgttcttcat cccatttcat ggagtttttc agcgaggtca tcgcccacgg
     6421 cgcgcgatca aggttgccgc gatcgacgta cagctccagt gctacttcgc gaccagaacg
     6481 cgtggtaaag gtatcgcgca gtacatcaaa gtcgcctgcc accagcgcaa acaggtagca
     6541 cggtttcggg aacgggtcct gccactgtac ccaatggcgt ccgttttcga gttcgccttg
     6601 cgcaacgcgg ttaccattgg aaagcaggaa gggatatttg attttatcgg caataatttt
     6661 ggtggtaaaa cgcgccagca cgtccgggcg gtcgagataa tacgtaatat ggcggaaacc
     6721 ttcggcttca cactgggtgc aaagcgcatc gcctgactga taaagccctt ccagcgccgt
     6781 attcgccgcc gggcttattt cattaatgat cttaagcgta aaacgctccg gcaaattact
     6841 gatgaccagt gcgccctctt cttctttcca ggcggtccac ggctcatcat taatatgaac
     6901 agaaaccagt ttgaggtctt cgccgttgag acgaagcgga gcatctgatg caccatgacg
     6961 gacagcctgg ctgaccgcgg taacgaccgt cttttgcgcg tcgaggtcaa aggtcaagtc
     7021 aatatcagta atctggtaat ccggcgcacg ataatcgtga cggtatttgg cttgtggctg
     7081 ttgagtcata aataaccttt agcatctttt atagagtctg gtgttcagtc tattcctgtt
     7141 gcgtaaatcg cgctatgcag aatcttcatc ttttcaggta caaacgcctt tattgctaca
     7201 tttttataac atacaccgcg taatgccatc gaccagaaag gtggcatatg gtgtgatcgg
     7261 ggttcaataa attgcgaaac aaggtatact ccagcagttc ctgaagatgt ttattgtact
     7321 aaacgctcct gtacgaggac gctactgcgc acctatgaca caattcgctt ctcctgttct
     7381 gcactcgttg ctggatacag atgcttataa gttgcatatg cagcaagccg tgtttcatca
     7441 ctattacgat gtgcatgtcg cggcggagtt tcgttgccga ggtgacgatc tgctgggtat
     7501 ttatgccgat gctattcgtg aacagattca ggcgatgcag cacctgcgcc tgcaggatga
     7561 tgaatatcag tggctctctg ctctgccttt ctttaaggcc gactatctta actggttacg
     7621 cgagttccgc tttaacccgg aacaagtcac cgtatccaac gataatggca agctggatat
     7681 tcgtttaagc ggcccgtggc gtgaagtcat cctctgggaa gttcctttgc tggcggttat
     7741 cagtgaaatg gtacatcgct atcgctcacc gcaggccgac gttgcgcaag ccctcgacac
     7801 gctggaaagc aaattagtcg acttctcggc gttaaccgcc ggccttgata tgtcgcgctt
     7861 ccatcttatg gattttggca cccgtcgccg tttttctcgc gaagtacaag aaaccatcgt
     7921 taagcgtctg caacaggaat cctggttcgt gggcaccagc aactacgatc tggcgcgtcg
     7981 gctttccctc acgccgatgg gaacacaggc acacgaatgg ttccaggcac atcagcaaat
     8041 cagcccggat ctagccaaca gccagcgagc tgcacttgct gcctggctgg aagaatatcc
     8101 cgaccaactt ggcattgcat taaccgactg catcactatg gatgctttcc tgcgtgattt
     8161 cggtgtcgag ttcgccagtc gctatcaggg cctgcgtcat gactctggcg acccggttga
     8221 atggggtgaa aaagccattg cacattatga aaagcttgga attgatccac agagtaaaac
     8281 tctggttttc tctgacaatc tggatttacg caaagcggtt gagctatacc gccacttctc
     8341 ttcccgcgtg caattaagtt ttggtattgg gactcgcctg acctgcgata tcccccaggt
     8401 aaaacccctg aatattgtga ttaagttggt tgagtgtaac ggtaaaccgg tggcgaaact
     8461 ttctgacagc cctggcaaaa ctatctgcca cgataaagcg tttgttcggg cgctgcgcaa
     8521 agcgttcgac cttccgcata ttaaaaaagc cagttaatat catcagggag ctaatcggct
     8581 cccttttttt acctttaatt ccgaaatctt tcgctgcatt tgcgaattct gcttgtctga
     8641 ttgcagatgc caggtaacat aggtatcccc ccatttacgg gtggacaagt gtttattttt
     8701 tccgactatt aacagagaga atattatgag cgttgtgcct gtagccgacg tactccaggg
     8761 ccgcgtagcc gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc gccgagattc
     8821 aaaagctggc atctccttcc tcgccgttta tgacggttcc tgctttgatc ctgtacaggc
     8881 tgtcatcaat aattctctgc ccaattacaa tgaagacgtc ctgcgtctga ccaccggctg
     8941 ctcggtcatt gtgacgggta aagtcgtggc gtcgccgggc caggggcaac aatttgaaat
     9001 tcaggccagc aaggttgaag ttgctggttg ggttgaagat ccagacactt acccgatggc
     9061 ggcaaaacgc cacagcattg agtatctgcg tgaagtcgct cacctgcgtc cgcgcacaaa
     9121 cctgattggt gccgtcgcgc gcgttcgtca tacgctggcg caggcgctgc atcgcttctt
     9181 taacgagcag ggattcttct gggtttcaac gccactgatt accgcatctg ataccgaagg
     9241 tgcaggcgaa atgttccgcg tttctacgct ggatctggaa aacctgccgc gtaacgatca
     9301 gggcaaagtg gatttcgaca aagacttctt tggtaaagag tctttcctga ccgtatctgg
     9361 ccagttgaac ggcgaaacct acgcttgcgc attatccaaa atttatacct tcggcccgac
     9421 tttccgtgct gaaaactcca acaccagccg tcacctggcg gaattctgga tgctggagcc
     9481 ggaagtggcg tttgctaacc tgaacgatat cgcgggtctg gctgaagcca tgctgaaata
     9541 tgtcttcaaa gcggttctcg aagaacgcgc agacgacatg aaattcttcg ctgaacgcgt
     9601 agataaagat gccgtttcac gtctggaacg cttcattgag gccgatttcg cgcaggtgga
     9661 ttacaccgac gcagtgacca ttctcgaaaa ctgcgacagg aagtttgaaa acccggttta
     9721 ctggggcgtc gatctctctt ctgagcatga gcgttatctg gcggaagaac actttaaagc
     9781 accggtagtg gttaaaaact atccgaaaga tattaaagcg ttctatatgc gcctcaacga
     9841 agatggtaaa accgttgcgg ctatggacgt tctggctccg ggcatcggtg agatcattgg
     9901 tggctcccag cgtgaagaac gtctggacgt tctggacgag cgtatgctgg aaatgggcct
     9961 gaataaagaa gattactggt ggtatcgcga tctgcgtcgc tacggtactg ttccgcattc
    10021 aggtttcggt cttggttttg aacgcctgat tgcttacgta actggcgtgc aaaacgtgcg
    10081 tgatgtgatt ccgttcccac gtactccgcg caacgccagc ttctgatttt cttcctcaaa
    10141 gagccagcgc ctgctggctc ttttttatcc tttctgtcaa gttatctgtt tgttaagtca
    10201 agcaatcaat ttgcaacccc gccataacga tcatcctgtt acggaatatt acattgcaac
    10261 atttacgcac aaaaaataac ccgcactctt attgcggatt agttttttct tagctgatag
    10321 cacaattttc atactatttt ttggcattct ggatgtctga aagaggattt tgtgccaggt
    10381 cgataaagtt tccatcaga
//
LOCUS       DAJSKJ010000135        10356 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000135 DAJSKJ010000000
VERSION     DAJSKJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.136"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            235..1623
                     /gene="narU"
                     /locus_tag="ON345_002941"
     CDS             235..1623
                     /gene="narU"
                     /locus_tag="ON345_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR3072971.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1705..5445
                     /gene="narZ"
                     /locus_tag="ON345_002942"
     CDS             1705..5445
                     /gene="narZ"
                     /locus_tag="ON345_002942"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCR3072972.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLSTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5442..6986
                     /gene="narH"
                     /locus_tag="ON345_002943"
     CDS             5442..6986
                     /gene="narH"
                     /locus_tag="ON345_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR3072973.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6986..7681
                     /gene="narW"
                     /locus_tag="ON345_002944"
     CDS             6986..7681
                     /gene="narW"
                     /locus_tag="ON345_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR3072974.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7678..8358
                     /gene="narI"
                     /locus_tag="ON345_002945"
     CDS             7678..8358
                     /gene="narI"
                     /locus_tag="ON345_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR3072975.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8438..9331
                     /locus_tag="ON345_002946"
     CDS             8438..9331
                     /locus_tag="ON345_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCR3072976.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGNQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9427..10272)
                     /gene="nhoA"
                     /locus_tag="ON345_002947"
     CDS             complement(9427..10272)
                     /gene="nhoA"
                     /locus_tag="ON345_002947"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCR3072977.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
BASE COUNT         2408 a         2742 c         2755 g         2451 t
ORIGIN      
        1 tgaaacatga catacgttca aagattaacc ccgtttgaag gttagcttgt tagttacatt
       61 tagtaacaca tatctggcta cttaatcctc attctgcccc cgcggttacg ctttcttatc
      121 tattctttca atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc
      181 cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca
      241 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
      301 ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt
      361 ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa tctcaataaa
      421 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
      481 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccgatgg
      541 acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
      601 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
      661 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
      721 agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
      781 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
      841 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
      901 attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
      961 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     1021 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     1081 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg
     1141 cggtcggttg gtggtgctat ttccgataag ttcggcggcg tgcgggtgac gttgatcaac
     1201 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     1261 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc gggtctggga
     1321 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggta
     1381 aagatgaaag gcggtagtga tgagcaagct cagaaagaag ccgtcaccga aacggcggcg
     1441 gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcgttt
     1501 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     1561 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     1621 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     1681 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     1741 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     1801 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt
     1861 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     1921 atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc
     1981 tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg
     2041 ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg
     2101 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg
     2161 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct gattgccgcc
     2221 gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc
     2281 ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcact gcttggcggc
     2341 acctgtttaa gcttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     2401 ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta catcatcgcc
     2461 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgc
     2521 tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc
     2581 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatgggccat
     2641 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     2701 cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg tagctacgtt
     2761 cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag caacaatccg
     2821 cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa cggttcgatt
     2881 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtacggaa
     2941 accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt ggccttcccc
     3001 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     3061 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc ggtggtcagc
     3121 gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagt
     3181 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     3241 gtgccgcgcc agtatattga aaccatcgcc cgtgaatttg ccgataccgc ccataaaacg
     3301 catgggcgct cgatgattat cctcggcgca ggtgttaacc actggtatca catggacatg
     3361 aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc
     3421 ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg gttgccactg
     3481 gcctttgcgc tcgactggaa ccgaccaccg cgccaaatga acagcacctc gtttttctac
     3541 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctc
     3601 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgtatg
     3661 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     3721 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     3781 cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg
     3841 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     3901 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     3961 gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac
     4021 ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgtccaccgc cacctggtac
     4081 gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct ttctgcggcg
     4141 gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat cgccaaagca
     4201 ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt acaaccccta
     4261 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     4321 gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     4381 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggc
     4441 ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa actcaattac
     4501 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     4561 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     4621 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     4681 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg
     4741 tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt tcatgagtta
     4801 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     4861 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     4921 atgcgccaga taccgccaaa cggcttcccg gaaaaagcac ttaacttcct gacgccgcac
     4981 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     5041 ggtggaccga ttgtctggat cagcgaaacc gatgcccgag aactgaccat tgtcgataac
     5101 gactgggtgg aagtatttaa cgccaacggc gcgctgactg cccgcgcggt ggtcagccaa
     5161 cgtgtaccgc cgggcatgac catgatgtat cacgcccagg aacgcattat gaatattcct
     5221 ggttcggaag taactggcat gcgcggcggc attcataact cggttacccg cgtttgcccg
     5281 aaaccgacgc atatgattgg cggttacgcg cagctggcct ggggctttaa ctactacggc
     5341 accgtcggct cgaaccgcga cgaattcatc atgatccgca aaatgaaaaa cgttaactgg
     5401 ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaatc cgttcacaag
     5461 tcggcatggt gcttaacctc gacaaatgta tcggctgcca tacctgttcg gtgacctgta
     5521 aaaacgtctg gaccgggcgc gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca
     5581 aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa ggcggctggg
     5641 tccgcgatgt gaatggcaag atccgtccgc gtctgggcag caagatgggc gtaataacca
     5701 aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct ttcactttcg
     5761 actacgaaca tttgcatagc gcaccggaag gcaaacatat ccctactgct cgcccgcgct
     5821 cgctgattga cggtaagcgg atggacaaag tgatctgggg gccaaactgg gaagaactgc
     5881 tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt cgaggccata caaaaggaga
     5941 tgtacgggca gtttgaaaac accttcatga tgtacctgcc gcgcctgtgc gaacactgcc
     6001 tcaatcccag ttgcgtggcg acctgcccaa gcggcgctat ctacaaacgc gaagaagacg
     6061 gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcatt agcggttgtc
     6121 cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt
     6181 atccgcgaat tgagtcaggc caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc
     6241 ggtatctggg cgtgctgctt tacgacgccg accgcatcga ggaagcggcg agcaccgagc
     6301 gcgaagttga cctctatgaa cgtcagtgcg aagtgttcct cgatccacac gatccctcag
     6361 tgatcgagga agccctgaaa caaggtattc cacaaaacgt gattgaagct gcccagcgtt
     6421 cgcctgtcta caaaatggcg atggactgga aactggcgct accgctgcac cctgaatatc
     6481 gcaccctgcc gatggtctgg tacgttcctc cgctgtcgcc gattcagtcc tacgcagatg
     6541 cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctt cgtattccgg
     6601 tgcaatatct cgccaatatg ttgagtgccg gcgataccgg tccggtactg cgggcgctga
     6661 aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc gttactgata
     6721 ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagagatg tatcgttacc
     6781 tcgccattgc caactatgaa gatcgttttg tcatcccgac gagccatcgg gaaatggcgg
     6841 gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt tgccacggtt
     6901 cggacagtaa attcaacctg ttcaacagta gccgtatcga tgccatcaac atcaccgaag
     6961 tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc tgttgatgga
     7021 gtatccggac gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga tccgcagcga
     7081 cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc tggataaaca
     7141 ggccgaatgg tgcgaagtgt ttgaccgcgg gcgcaccacg tcgctgctgc tgttcgaaca
     7201 tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg cggagtatga
     7261 aaaggtcggc ctgcagctgg attgtcggga actgcccgat tatctaccgc tgtatctgga
     7321 gtatttaagc gtactgcctg acgatcaggc gaaagaagga ttgctcaatg ttgcgccgat
     7381 cctcgccctg cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga
     7441 tgctctgctg caactggcgg gaagtacact ttcaagtgac agtgtcacaa aacaagtaaa
     7501 cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag aggaacaggt
     7561 gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg
     7621 acgctttagc caggatgtcg cgccgcagta tgtcgatatc agtgcgggag gtgggaaatg
     7681 attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc gacggtgttt
     7741 ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc ctcctcaagt
     7801 caaatgctcg ataaacgcgg gatggtgata tggtcgaatc tgttccatat tggcattttg
     7861 gggattttct tcgggcacct gttcggcatg ttaacgccgc actggatgta cgcgtggttt
     7921 ttgccggtgg cagcgaaaca actaatggcg atggtgctcg gaggcatttg cggtgtgttg
     7981 acgttgattg gtggcgcagg actgctgtgg cgcaggctga caaatcagcg cgtgcgtgcc
     8041 acttccacta cgccggatat catcatcatg agcattttgc tggtccagtg tctgttgggg
     8101 ctaagcacca taccgttttc agcacagtat cctgatggta gcgaaatgat gaagctggtg
     8161 ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagagatgct taacggtgta
     8221 gcgttcgtct tccgcctgca tctggtgttg ggaatgacca tcttcctgct cttcccgttc
     8281 acccgactgg tgcatgtgtg gagcgccccg tttgagtact ttactcgtcg gtatcagatt
     8341 gtgcgctcgc ggcgataatt ttcaccctgg ccggatagcc cttcgctctc cggccacttg
     8401 caggctatga tagctctcca gttacaggag aacgttaatg aaaccgcagg tttaccacgt
     8461 cgatgctttt acctctcaac catttcgcgg caattctgcc ggtgttgttt tccccgccga
     8521 taatcttagc gaagcgcaaa tgcagcttat cgcccgcgaa ttaggtcatt cggaaacggc
     8581 ttttctgctg cacagcgacg acagcgatgt gcgcatccgc tactttacgc caacggttga
     8641 agttccgatt tgtggtcacg cgacagtagc tgcgcactat gtacgtgcga aggtgttagg
     8701 tttaggaaat tgcacggtct ggcaaacatc gctggcagga aaacatcgcg tgactatcga
     8761 aaagcaccac gatggttatc gtatttcgct ggaacaaggt acgccgggct ttgaaccgcc
     8821 gctggagggt gaagtacgcg cggcgattat caacgcattg catcttactg aagacgatat
     8881 tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat tctaaagtga tgatcccgtt
     8941 gaagccggaa gtggatatcg acgctctttc gcctgacctt aatgcgctga ccgctatcag
     9001 caaacagatt ggctgcaatg gtttcttccc gttccagatc cgcccaggca aaaacgaaac
     9061 cgatggtcgc atgttctcgc ctgcgattgg tattgtggaa gatccggtga ctggcaatgc
     9121 caatggcccg atgggtgcat ggttggtaca tcacaacgta ttgccccacg atggcaaggt
     9181 gctgcgtgtt aaaggcaatc aggggcgcgc attggggcgt gacggcgtga ttgaagtgac
     9241 ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt tctggcgcgg cggtgatttt
     9301 gtttcatgcc gaatgggcaa ttaaactctg aaaccgttgc ctgatgcgct tcgcttatca
     9361 ggtctacaac cgagctaatg tatgccggat aaggcgttta cgccgcatcc ggcaacctga
     9421 cataaattat tttcccgcct ccgggtgcgt atcaaaagcc gccatcacca gcgctaactc
     9481 atccacggta aagccatgtt tcgcatcatc cacgcccaga ccaaactgtt cttgcatcac
     9541 agcatataat gacgccacat ccggtagatt tcgctgctcc accgcgtgcc cattttcata
     9601 atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg tccggcaaat ggcggcacat
     9661 cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac
     9721 ataatcgctt tgttgctgct cgcagagatc aaaacggtac atcgactgcc agtgctgatg
     9781 atgattaaac tgcaacaccc aatcatcacc ctcctgcaac aaccgataat ctccgtgtgg
     9841 cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa
     9901 accgacatca gcaatccatt tttcctcttc cagttccacc aacagcaaac gatgggtgcg
     9961 cggcggtaat gctggcggat ttgataacac tacgcgccct aacaagctgc gaacgttaaa
    10021 ctccagctcg cgtaacaccc gctcaaacac gccattctgc tcaaaacagt aaccgccacg
    10081 acgggctatc accagtttct cttccagcga ttgatcatca agctgtattt ccctcggcag
    10141 caaaacgtcg aggttttcaa acggaatagt gcaattgtgt ttcaggtgca atgcacgaag
    10201 cgtatcaata ttgaccgcag cagctcccga ccagttaata cgggcaaaat agtgattcag
    10261 aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa cttataacct ggaatagacg
    10321 ccgcaccggc tattttgtgc gatcgcggac atgcat
//
LOCUS       DAJSKJ010000136        10319 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000136 DAJSKJ010000000
VERSION     DAJSKJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.137"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(227..2800)
                     /gene="clpB"
                     /locus_tag="ON345_002948"
     CDS             complement(227..2800)
                     /gene="clpB"
                     /locus_tag="ON345_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR3072978.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2930..3661)
                     /gene="pgeF"
                     /locus_tag="ON345_002949"
     CDS             complement(2930..3661)
                     /gene="pgeF"
                     /locus_tag="ON345_002949"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR3072979.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTDEPYDSKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDCCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3658..4638)
                     /gene="rluD"
                     /locus_tag="ON345_002950"
     CDS             complement(3658..4638)
                     /gene="rluD"
                     /locus_tag="ON345_002950"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR3072980.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4773..5510
                     /gene="bamD"
                     /locus_tag="ON345_002951"
     CDS             4773..5510
                     /gene="bamD"
                     /locus_tag="ON345_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR3072981.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     PDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5781..6122
                     /gene="raiA"
                     /locus_tag="ON345_002952"
     CDS             5781..6122
                     /gene="raiA"
                     /locus_tag="ON345_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR3072982.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6225..6350
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6226..6273
                     /gene="pheL"
                     /locus_tag="ON345_002953"
     CDS             6226..6273
                     /gene="pheL"
                     /locus_tag="ON345_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR3072983.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6373..7533
                     /gene="pheA"
                     /locus_tag="ON345_002954"
     CDS             6373..7533
                     /gene="pheA"
                     /locus_tag="ON345_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR3072984.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ON345_002955"
     CDS             complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ON345_002955"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR3072985.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ON345_002956"
     CDS             complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ON345_002956"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR3072986.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9988..>10319
                     /locus_tag="ON345_002957"
     CDS             9988..>10319
                     /locus_tag="ON345_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR3072987.1"
                     /translation="MMKKFIAPLLALLVSGCQINPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGA"
BASE COUNT         2634 a         2743 c         2422 g         2520 t
ORIGIN      
        1 tttttgcttt ctctgtcgga attctcagga gaaccccgct gacccggcgg tgtgtttgcc
       61 gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac aagcgaaaaa
      121 tataaaaact ttatcgttcg ctcacttttc aggcaaaata tcttaaatat agtcttttcc
      181 gtctaactta tagacaaaaa cgagccccga agggctcgtt ttatcattac tggacagcga
      241 caatccggtc ttcattaact tccaggcgaa tcactttacc cggaaccaat tcaccagaca
      301 gtatttgctg tgccagcggg ttttcgatct gctgctggat agcacgtttc aaaggacgtg
      361 caccatagac tggatcgtaa ccgttctcgc tcagcagttt cagcgcctcg tcagaaatgt
      421 ggatttcata accacgttct tccagacgtt tgtacagacg tttcaactga atctgcgcaa
      481 tcgaggcaat gtgctgttca cccagcggat ggaagaccac cacttcatca atacggttaa
      541 tgaattccgg acggaagtta tggcttacca caccgagcac cagctctttc atgtgcgcat
      601 aatccagttc accgaagcgt tcctgaatca aatcggaacc gaggttagag gtcataatga
      661 caaccgtatt acggaagtcg accgttctcc cctgcccgtc agtcagacga ccatcatcca
      721 gtacctgcaa cagaatgttg aagacatccg gatgcgcttt ttccacttca tccagcagga
      781 tgacggaata cggacgacga cgcacagctt cggtcaggta gccaccttct tcataaccga
      841 catatcccgg aggcgcacca accagacgag acaccgagtg tttctccata aactcggaca
      901 tatcgatacg gaccatcgcc tcgtcgctat caaacataaa gttcgccagc gccttacaaa
      961 gctcagtttt ccccacaccc gttgggccga ggaacaggaa tgaaccaatc gggcgatttg
     1021 gatccgccaa ccccgcacgg ctacggcgaa tagcgttaga taccgcatca accgcttcgt
     1081 tctgaccaat gacgcgatgg tgcagttctt gctccatacg cagcagtttt tcgcgctcgc
     1141 tttccatcat gcgagaaacc ggaatccccg tccaacgcgc cagcacttca gcaatttcgg
     1201 cgtcggtcac tttattacgc aacagacgca tagttttgcc ttcgagctgc gttgcggctt
     1261 ccagttgctt ttccagttcc gggattttgc cgtattgcag ttcagacatc cgcgccaggt
     1321 cccccacacg gcgagcctgt tcaatagcga ttttcgcctg ttccagttcc gctttaatgg
     1381 tctgcgtacc agaaagcgat gccttctctg ctttccactc ttcttctaac tcggagtact
     1441 gacgttcttt gtcgctcagt tcttcgttga gcatatccag gcgtttttta ctggcttcat
     1501 cagactcttt cattaacgcc tgttgttcca gtttgagctg gatgatacga cgatcgagtc
     1561 ggtcgagttc ttctggtttt gagtcaatct gcatacgaat gctggatgct gcttcatcga
     1621 tcaggtcgat ggctttatcc ggcagctgac ggtcagcaat gtagcgatga gacaacgtcg
     1681 ctgctgcaac aattgccggg tcagtaattt gcacatggtg gtgcaattcg taacgttctt
     1741 tcaggccacg cagaatcgca atggtatctt caacagaagg ctcggcaaca aacactttct
     1801 ggaaacgacg ttccagcgca gcatcttttt cgatgtactg gcgatattcg tcaagcgtcg
     1861 tggcacctac gcagtgcaat tcaccacgcg ccagcgccgg tttcagcatg tttccggcgt
     1921 ccattgcgcc atcggcttta cccgcgccga ccatggtatg taattcgtcg ataaacagga
     1981 tgacgttgcc ttcctgtttg gcaagatcgt taagcacgcc ttttaaacgt tcttcaaact
     2041 caccgcgata tttcgcccca gccaccagcg cgcccatatc cagcgccagt acgcggcggc
     2101 ctttcaatcc ttccggcact tcgccgttga taatacgctg cgccagacct tcaacgatgg
     2161 cagttttacc gacgccgggt tcaccaatca gtaccgggtt atttttagta cgacgttgca
     2221 gcacctgaat ggtacggcga atttcttcat cacgaccaat caccggatcg agtttgccct
     2281 gttcggctcg ttcggtaagg tcgatggtat attttttcaa agcctgacgt tggtcttcag
     2341 caccttgatc gttcacgctt tcacctccac gcatttgttc aatcgcttga gtaatgttgg
     2401 cggtggtcgc ccctgctgct ttcaggatgt cggccagcgt gccgcgagac tcaagtgccg
     2461 ccagaacgaa cagttctgac gagataaagt tatcaccacg tttttgcgcc agcttgtcgc
     2521 aaagattaag aacgcgcacc agatcctgtg atggctggac atcaccacca gtaccttcaa
     2581 cctgcggtaa acgatttaat gcctgattga tatctgtgcg caactggcca gcatttatgc
     2641 cagcggatgt taataaagga ctaaccgaac ccccttcctg attcagcagg gcgctcatta
     2701 aatgaagtgg ttcgatgaat tggttgtcgt gcccgagtgc aagtgattgg gcatcggcaa
     2761 gagcaagctg gaatttatta gtaagacgat ccagacgcat aactcctccc ataacggatc
     2821 aaagttgcta ctggagatta aatgaggtca tccctcaatt attcaaggtt attgaccaga
     2881 ttaatgtgaa aagaaaatca cgcgtaccgg atcgtcttga ttctttaggt tatatcagcc
     2941 aaatgaaact tgccatacga ccggtggtct tgtcgcgacg ataagagaag aaagtctcat
     3001 tttccgtata tgtacaacag tcgccgccga aaatttgctc aacaccaacg ctcgccagac
     3061 gctgccgggc aagctgataa atgtccgcca gatacttatc accatgctga atgaaagctg
     3121 tactcgcttc ggcgtctact gccataaacg cctcgcgaac ctccgccccc acttcaaacg
     3181 cgcgtggacc aattgccggc cctaaccaag cgagaatatt ttctggatta tcagcaaaac
     3241 aggaaactgt ctcttccagc acgcctgcgc acagtccacg ccagccagca tgagcggcgg
     3301 cgacttccgt tcccgctcga ttgcaaaaca gcacagggag gcagtcggca gtcatcactg
     3361 cgcaaaccgt gccgggcgta ttgctataag aggcatctgc tcgttttgag tcataaggtt
     3421 cgtcagtaag cttaagcaca tctttgccgt gtacctgctc aagccagacc ggtttagaag
     3481 gcaaattgcc cgcagcaaaa agtcgcttgc gattctcctc aacgtgatcc gggttatcgc
     3541 cacaatgggc accgaggttg agcgagtcat acgggggcaa gctcacgccg ccgatacgag
     3601 tagagctaca ggccgcaaca ccttttggca gcggccactg cgggacaatc agcttactca
     3661 taaccagtcc acttcatctt tatgctcttc gaaatcggcg cgcatcacct caatcagctc
     3721 caccatatct tgtggaatag gcgcatgcca ttccatttcg ataccggaga tcgggtgata
     3781 aagacgcagc atggttgcat gaagcgcctg gcggtcaaac ttacgcagcg tggagataaa
     3841 cgcttccgaa gcaccttttg gcggacgcgg acggccacca taaaccggat cgcccaccag
     3901 cggatgagtg atatgggcca tatgcacgcg gatctggtgc gtacgtccag tttccagacg
     3961 caaccgcaga cgcgtgtgca cacggaagtg ttccatgatg cgatagtgag tcaccgccgg
     4021 tttgcccatc ggatgcaccg ccatgtgagt acgtttggtc gggtggcgac tgattggctc
     4081 atccaccgta ccgccagcgg tcatatgacc aatcgccacc gcttcgtact cacgagtaat
     4141 ttcacgcctt tgcaaagatt cgactaaacg cgtctgagcc ggaacggttt ttgccacaac
     4201 catcaggcca gtggtgtctt tatccagacg atggacgatg cccgcacgcg gtacatcggc
     4261 aataggcgga taataatgaa gcaacgcatt cagtaccgtg ccatccgggt tacccgcgcc
     4321 aggatgaacc accaggtcgc gcggtttatt aatgacgatg atgtcttcat cttcatagac
     4381 gatatccagc gggatatcct gcggttcaaa acgcgcttct tcttcaatct ctgcgttgat
     4441 ggcaacctgc tcgccaccca atactttttc tttcggctta tcacaaagtt tgccgttaac
     4501 cagcacgcgc tggtcgagga tccattcttt tattcgcgaa cgtgaataat ccgggaacat
     4561 ttcggccaaa gcctgatcta agcgttgtcc gagttggttt tcggacaccg ttgcagtgag
     4621 ctgtactcgt tgtgccatat atactgcttc ttcgtttatc gttggggttt tacggctttg
     4681 ccgtttaata tagtgtgcta ttgtagctgg tcttaaccgg gagcaggaac agagaatctc
     4741 ccgtattaca ttttgaggaa agtcaaaacg tcatgacgcg catgaaatat ctggtggcag
     4801 ccgccacact aagcctgttt ttggcgggtt gctcggggtc aaaggaagaa gtacctgata
     4861 atccgccaaa tgaaatttac gcgactgcac aacaaaagct gcaggacggt aactggagac
     4921 aggcaataac gcaactggaa gcgttagata atcgctatcc gtttggtccg tattcgcagc
     4981 aggtgcagct ggatctcatc tacgcctact ataaaaacgc cgatttgccg ttagcgcagg
     5041 ctgccatcga tcgttttatt cgcctaaacc cgacccatcc gaatatcgat tatgtcatgt
     5101 acatgcgtgg cctgaccaat atggcgctgg atgacagtgc gctgcaaggg ttctttggcg
     5161 ttgaccgtag cgatcgcgat cctcaacatg cacgagctgc gtttagtgac ttttccaaac
     5221 tggtgcgcgg ctatccaaac agtcagtaca cccccgatgc caccaagcgt ctggtattcc
     5281 tgaaagatcg tctggcgaaa tatgaatact ccgtagccga gtactataca gaacgtggcg
     5341 catgggttgc cgtcgttaac cgcgtagaag gcatgttgcg cgactacccg gatacccagg
     5401 ctacgcgtga tgcgctgccg ctgatggaaa atgcataccg tcagatgcag atgaatgcgc
     5461 aagctgaaaa agtagcgaaa atcatcgccg caaacagcag caatacataa cagaaacctg
     5521 gaacacaaaa cggcagccct tgagctgccg tttttttatt ctgttagttg tgaaactgaa
     5581 gcgatttagt cactatcgat ctcatcaaat atggctcgct ttgagatatt cctcaagtaa
     5641 aaaaacacct cttcctgcga tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat
     5701 tatgagattc ccatcacaca ttttgacatc aggaacggta tgctgaattc accaagacgg
     5761 gaagacaaga ggtaaaattt atgacaatga acattaccag caaacaaatg gaaattactc
     5821 cggccatccg ccaacatgtc gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc
     5881 tgattaatcc acatatcatt ctgtccaaag agccacaagg gtttgttgct gacgccacaa
     5941 tcaatacacc taacggcgtt ctggttgcca gtggtaaaca tgaagatatg tacactgcaa
     6001 ttaacgaatt gatcaacaag ctggaacggc agctcaataa actgcagcac aaaggcgaag
     6061 cacgtcgtgc cgcaacatcg gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt
     6121 agtcctttat attgagtgta tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt
     6181 gaaaacagta cgggtactgt actaaagtca cttaaggaaa caaacatgaa acacacaccg
     6241 tttttcttcg cattcttttt taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca
     6301 gaatgcgaag acgaacaata aggcctccca aaccgggggg ccttttttat tgataacaaa
     6361 aaaggcaaca ctatgacatc ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg
     6421 ctggatgaaa aattattagc attactggca gagcggcgcg aactggccgt cgaggtggga
     6481 aaagccaaac tgctctcgca tcgcccggta cgagatattg atcgtgaacg cgatttactg
     6541 gaaagattaa ttacgctcgg taaagcgcac catctggacg cccattacat tactcgcctg
     6601 ttccagctca tcattgaaga ttccgtatta actcagcagg ctttgctcca gcaacatctc
     6661 aataaaatta atccgcactc agcacgcatc gcttttctcg gccccaaagg ttcttattcc
     6721 catcttgcgg cgcgccagta tgctgcccgt cactttgagc aattcattga aagtggctgc
     6781 gccaaatttg ccgatatttt taatcaggtg gaaaccggcc aggccgacta tgccgtcgta
     6841 ccgattgaaa ataccagctc cggtgccata aacgacgttt acgatctgct gcaacatacc
     6901 agcttgtcga ttgttggcga gatgacgctg accatcgacc actgcctgct ggtttccggc
     6961 accaccgatt tatccaccat taatacggtc tacagccatc cgcagccatt ccagcaatgc
     7021 agcaaattcc ttaatcgtta tcctcactgg aagattgaat ataccgaaag tacgtctgcg
     7081 gcaatggaaa aggttgcaca ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct
     7141 ggcggcactt tgtacggttt gcaggtactg gagcgtattg aagcgaatca gcaacaaaac
     7201 ttcacccgat ttgtggtgtt ggcacgtaaa gccattaacg tgtctgacca ggttccggcg
     7261 aaaacgacgt tgttaatggc gaccgggcaa caagccggtg cgctggttga agcgttgctg
     7321 gtgctgcgca accacaatct gattatgacc cgtctggaat cacgcccgat tcacggtaat
     7381 ccatgggaag agatgtttta tctggatatt caggccaatc ttgaatcagc ggaaatgcaa
     7441 aaagcattga aagagttagg ggaaattacc cgttcaatga aggtattggg ctgttaccca
     7501 agtgagaacg tagtgcctgt tgatccaacc tgatgaaaag gtgccggatg atgtgaatca
     7561 tccggcactg gattattact ggcggttgtc attcgcctga cgcaataaca cgcggctttc
     7621 actctgaaaa cgctgtgcgt aatcgccgaa ccagtgctcc accttgcgga aactgtcaat
     7681 aaacgcctgc ttatcgccct gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta
     7741 acgtttgatt aacgccagat tacgctctga cgacataata atgtcggcat aaagctgcgg
     7801 atcctgagca aacagtcgcc cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag
     7861 cgccagaagt tgctcaagct gaacattttc ttctgccaga tgcagcccat aagcaaaagt
     7921 agcaaagtgg cgcagagcct gaataaacgc catattctga tcgtgctcga cagcgctaat
     7981 acgatgcagc cgagcgcccc agacctgaat ttgctccaga aaccattggt atgcttccgg
     8041 cttacgtcca tcacaccaga ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa
     8101 catcgggtgt aaccccagta ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc
     8161 atttttcact gatgccagat caaccagaat acaatctttc ggtaaaggcg gtaatttgcc
     8221 gataacttgc tcagtaacgt ggattggcac actaacaatc accattccgg catcggcaac
     8281 aatatcagcc gctcgatccc agtcatgttg ctccagaatc cgcacctgat aacccgagag
     8341 tgtcagcatc ttctcgaaca ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg
     8401 acgcagtgac ggacaaagtg ttttaaatcc tttgtcgttt tcactggagt aagattcacg
     8461 catcacccga cgcaaaacat cctcaatcag atctggcggt acacccagag cttccgcctc
     8521 tgcacgacgc gaggccaaca tagatgcctc gcgctccgga acataaatag gcagtccaaa
     8581 gcggcttttc acctcgccca cttcagcaac cagttccaga cgcttcgcta ataaattcag
     8641 cagcgcttta tcgacttcat caatttgatc gcgtaatgcg gtcaattcag caaccataat
     8701 aaacctctta agccacgcga gccgtcagct gcccgttcag atcctgatga atttcacgca
     8761 gcaaggcatc ggtcatttcc cagctaatgc aggcatcggt tacggataca ccgtatttca
     8821 tttcactgcg cggttgctcg gaagactgat tgccctcgtg gatattactt tcgatcatca
     8881 gaccaataat tgagcgattg ccatctttga tttgagcaac cacggattct gccaccgcag
     8941 gctgacggcg ataatcttta ttggaattac cgtggctgca atctaccatc agagacgggc
     9001 gcagtcccgc ctgttccatc tctttttcac attgcgcaac atccgcaggg ctatagttcg
     9061 gcgctttacc accgcgcagg atcacatggc cgtccggatt cccctgagtt tgtagcaacg
     9121 caacctgccc tgcctggtta atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag
     9181 cgttaattgc tgttgccaga ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa
     9241 gcccggaggc catttcacgg tgagtttgcg attccgttgt acgagcacca attgctgacc
     9301 agctaaacag atcgcccagg tattgcgggc tattcggatc taacgcttcc gtcgccagtg
     9361 gcagtcccat attcaccagc tcaagcagca atttacgcgc gatctgcagc ccggcttcta
     9421 catcaaaaga gccatccata tggggatcgt taattaaccc tttccagccg acagtggtac
     9481 ggggtttttc aaaatagacg cgcattacca gatagaggct atcgctgacc tctgcggcaa
     9541 gggctttaaa tcgacgagca tattccagag cagtttccgg atcatgaatg gaacaaggac
     9601 cacatactac cagcagacga ggatcgcgcc cggcgataat atctgaaatg gttctacgcg
     9661 agtcagcaat ctgggcttct tgttgcaggc tcaatggaaa agcggccttc agttgttccg
     9721 gagtcattaa aacctgttcg tcggtaatat gtacgttatt cagcgcgtct ttttgcatga
     9781 tggcgatcct gtttatgctc gtttgcgata gttgatcctc agcgaggatg acgtaacgat
     9841 aacacataaa gtaaagtttt caatccatat ttcgtacatt tttatttaca caggcaattt
     9901 agtcgcgatt tcaaccctta cttctgtata gataaattta cactcccttt gaaaacaatc
     9961 cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct
    10021 ttactggtta gcggatgtca gattaatcct tatactcacg cgccaacctt gaccagcacc
    10081 gactggtatg atgttggtat ggaagatgcg atatcgggca gcgccataaa agatgacgat
    10141 gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc cgaaggacaa
    10201 aagaaaactt gccagaccga ttttacttat gcccgaggac tttccggtaa aagctttcct
    10261 gccagctgca acaatgttga aaatgccagc caactgcatg aagtatggca aaaaggggc
//
LOCUS       DAJSKJ010000137        10233 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000137 DAJSKJ010000000
VERSION     DAJSKJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.138"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(23..280)
                     /locus_tag="ON345_002958"
     CDS             complement(23..280)
                     /locus_tag="ON345_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072988.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            403..2601
                     /gene="speF"
                     /locus_tag="ON345_002959"
     CDS             403..2601
                     /gene="speF"
                     /locus_tag="ON345_002959"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR3072989.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2598..3917
                     /gene="potE"
                     /locus_tag="ON345_002960"
     CDS             2598..3917
                     /gene="potE"
                     /locus_tag="ON345_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR3072990.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVRAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3973..5613)
                     /gene="pgm"
                     /locus_tag="ON345_002961"
     CDS             complement(3973..5613)
                     /gene="pgm"
                     /locus_tag="ON345_002961"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR3072991.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5639..6184)
                     /gene="seqA"
                     /locus_tag="ON345_002962"
     CDS             complement(5639..6184)
                     /gene="seqA"
                     /locus_tag="ON345_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR3072992.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6369..7133
                     /gene="ybfF"
                     /locus_tag="ON345_002963"
     CDS             6369..7133
                     /gene="ybfF"
                     /locus_tag="ON345_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3072993.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHMIAGAGHWVHVEKPDAVLRAIRRYLND"
     gene            7273..7566
                     /gene="ybfE"
                     /locus_tag="ON345_002964"
     CDS             7273..7566
                     /gene="ybfE"
                     /locus_tag="ON345_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR3072994.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7706..8236
                     /gene="fldA"
                     /locus_tag="ON345_002965"
     CDS             7706..8236
                     /gene="fldA"
                     /locus_tag="ON345_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR3072995.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8446..8532
                     /gene="uof"
                     /locus_tag="ON345_002966"
     CDS             8446..8532
                     /gene="uof"
                     /locus_tag="ON345_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR3072996.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8525..8971
                     /gene="fur"
                     /locus_tag="ON345_002967"
     CDS             8525..8971
                     /gene="fur"
                     /locus_tag="ON345_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR3072997.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9056..9382)
                     /locus_tag="ON345_002968"
     CDS             complement(9056..9382)
                     /locus_tag="ON345_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3072998.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9432..>10233)
                     /gene="chiP"
                     /locus_tag="ON345_002969"
     CDS             complement(9432..>10233)
                     /gene="chiP"
                     /locus_tag="ON345_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR3072999.1"
                     /translation="HLEMDEFYQNDKTTKVDYLHSLGAKYDFKNNFVLEAAFGQAEGY
                     IDQYFAKASYKFDIAGSPLTTSYQFYGTRDKVDDRSVNDLYDGTAWLQALTFGYRAAD
                     VVDLRLEGTWVKADGQQGYFLQRMTPTYASSNGRLDIWWDNRSDFNANGEKAVFFGAM
                     YDLKNWNLPGFAIGASYVYAWDAKPATWQSNPDAYYDKNRTIEESAYSLDAVYTIQDG
                     RAKGTMFKLHFTEYDNHSDIPSWGGGYGNIFQDERDVKFMVIAPFTIF"
BASE COUNT         2456 a         2553 c         2569 g         2655 t
ORIGIN      
        1 aatatcttca ccttcattca catcaggaaa ggtaaattaa atggaaaata acagccgcac
       61 tatgccccat ataaggcgga caactcatat tatgaagttt gctcatcgca atagcttcga
      121 ctttcacttc ttcaatgccc gttagtctac cgactaaggg cacttcagcg tacaggtctt
      181 cctgactctc tgtattacag gcatatgcct gtattccgca gtgctcattc tctgttaacc
      241 cttgtattat cagccaccgg actggtaagg gtttctgcat tctgaaaaaa gcaatctacc
      301 tgatttattt atcagcgggg taactttgct tttttccggc acgatcgatt tctcattcga
      361 gaaattgagg acctgctatt acctaaaata aagagatgaa aaatgtcaaa attaaaaatt
      421 gcggttagtg attcttgccc ggactgtttt accacgcagc gagaatgtat ctacattaat
      481 gaaagtcgta atatcgatgt ggcggcaata gttttatcgc tcaacgatgt tacatgcgga
      541 aaactcgatg aaatcgatgc cacgggttat ggcatcccgg tatttattgc tactgaaaat
      601 caagaacgtg tacccgcaga gtatttgccc cgtatttcgg gtgtctttga gaattgcgaa
      661 tcgcgacgag aattttatgg tcgtcagtta gaaaccgctg ccagccatta tgaaactcaa
      721 ctgcgcccac ctttcttccg cgcactggtc gattatgtca atcaaggtaa cagcgcgttt
      781 gattgccctg gtcatcaggg cggcgaattt ttccgtcgcc atccggcggg gaatcagttt
      841 gtggaatact ttggtgaggc gctgttccgt tccgacttgt gcaacgcaga cgtagcaatg
      901 ggtgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg
      961 ttcaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa caaagtggtt
     1021 ttaaacgccc tgctgacacc gggtgatctg gtgctgtttg accgcaataa ccacaaatct
     1081 aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga aaccgcacgt
     1141 aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt
     1201 gagctgatcg cggaagtcgc accgcagcgg acaaaagagg ctcgtccttt ccgcctcgcc
     1261 gttattcagc taggcaccta cgacggtacg atttataacg cccgccaggt ggtggataaa
     1321 attggtcatc tgtgtgacta cattctgttt gactccgcat gggtcggcta tgagcaattt
     1381 atcccgatga tggctgactg ttcaccgctg ttgctggatc ttaatgagaa cgatccgggc
     1441 attctggtta cgcaatctgt gcataaacag caggctggtt tttctcaaac ttcacaaatc
     1501 cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
     1561 aacgccttta tgatgcatgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
     1621 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta
     1681 aatggcatta atgcccgcaa gctgatcctc gataactgtc agcatattcg tccgttcgta
     1741 cctgaactag tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
     1801 ctgcgcttct tccagtttgt accaggggaa cactggcact cttttgaagg ctatgcagag
     1861 aatcaatact ttgtcgatcc atgcaaactg ttgctgacaa ccccaggtat tgatgcacgt
     1921 aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgcgaa
     1981 aatggcgtag tgccggaaaa atgcgatctt aactccatcc tcttcctgct gactccggca
     2041 gaagatatgg ccaaacttca gcaacttgtt gccctgctgg tacgcttcga aaaactgctt
     2101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
     2161 tacgccggtt atacccttcg tcagttgtgc caggaaatgc atgatttgta tgcccgtcac
     2221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
     2281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
     2341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
     2401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
     2461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
     2521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
     2581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
     2641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
     2701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
     2761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
     2821 atgccgagta tgctttcggt aaatccggta actttatggc gaactatacc tacggcgtct
     2881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
     2941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
     3001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
     3061 ggggggtcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
     3121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
     3181 ccatcgctat gacgctgtgg gcttttctcg gtctggagtc tgcgtgtgcg aatactgatg
     3241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
     3301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
     3361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gttcacacca gaagtgggta
     3421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
     3481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
     3541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
     3601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
     3661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
     3721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttc
     3781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
     3841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
     3901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gccctttttt
     3961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
     4021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
     4081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccgat
     4141 agaagcacca ttgcccggtg ccgccgtcag gcgcgcggtg atcgggtcgc ctgccagggt
     4201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
     4261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
     4321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
     4381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
     4441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
     4501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
     4561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
     4621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
     4681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
     4741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
     4801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
     4861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
     4921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
     4981 gaacggctgc accagatcct gctctttcac atgaccggat gccatcgctt cgtcgaggga
     5041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
     5101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
     5161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
     5221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
     5281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
     5341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
     5401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
     5461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
     5521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
     5581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
     5641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
     5701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggtgtgc
     5761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
     5821 caaagtaaac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
     5881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
     5941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
     6001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
     6061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
     6121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
     6181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaggagta tatgaataaa
     6241 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
     6301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
     6361 actttgccat gaaattgaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
     6421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
     6481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
     6541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
     6601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
     6661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
     6721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
     6781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
     6841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
     6901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
     6961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
     7021 tggcacaatt tccacaggca cgggcgcata tgattgcagg cgcgggtcac tgggtccatg
     7081 ttgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
     7141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
     7201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
     7261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
     7321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
     7381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
     7441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
     7501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
     7561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
     7621 cgactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
     7681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
     7741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
     7801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttatgaca ttctgctgct
     7861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
     7921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
     7981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
     8041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
     8101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
     8161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
     8221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
     8281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
     8341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
     8401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
     8461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
     8521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
     8581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
     8641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
     8701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
     8761 atttgaactg acacagcaac atcaccacga tcacctgatc tgcctcgact gcggcaaggt
     8821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
     8881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
     8941 cgaagatgaa catgcgcacg aaggcaaata agccagcctg aatgagaaaa agccaacctg
     9001 cgggttggct tttttatgca agggaaataa gaaaagcgag agttacagct ctcacttatt
     9061 tgttattact gcgaatttcc tgccaaactt tatcacaatc ggctttcacc gcctgatcat
     9121 ttccggtttg cgttgcacgc agacactgct gataatccag tacgcggaca ctttcctggt
     9181 cggcaaaatg ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt
     9241 caattttttc tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc tctttcagac
     9301 gactgtcttc ctgtggcgcg gttgattgcg cacaagctac cagccccgat gccattatgg
     9361 cgatgagaat cagttttttc attgaaaatt cctcaaacgt gcggcaggca caaaacctgc
     9421 cgcgtcgtgc atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca
     9481 tcctggaaga tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat
     9541 tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc
     9601 gcatccaggc tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta cgcatccgga
     9661 ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg
     9721 aagcctggaa gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg
     9781 ccgttggcgt tgaagtcaga acggttatcc caccagatat ccaggcgacc gtttgaggaa
     9841 gcgtaggttg gagtcatacg ttgcaggaag tatccctgct gaccgtcagc cttaacccag
     9901 gtgccttcga ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc
     9961 agccaggcgg tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta
    10021 ccgtagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct
    10081 ttggcaaaat attgatcgat atacccttcc gcctgaccaa atgctgcttc cagtacgaag
    10141 ttatttttga agtcgtattt cgccccaagg gagtgcagat aatcaacttt ggtggtttta
    10201 tcgttctgat aaaactcatc catttccaga tgc
//
LOCUS       DAJSKJ010000138        10231 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000138 DAJSKJ010000000
VERSION     DAJSKJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.139"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            51..1445
                     /gene="galP"
                     /locus_tag="ON345_002970"
     CDS             51..1445
                     /gene="galP"
                     /locus_tag="ON345_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR3073000.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWHWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQIMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1522..2019
                     /locus_tag="ON345_002971"
     CDS             1522..2019
                     /locus_tag="ON345_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR3073001.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2114..2821
                     /gene="endA"
                     /locus_tag="ON345_002972"
     CDS             2114..2821
                     /gene="endA"
                     /locus_tag="ON345_002972"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR3073002.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2901..3632
                     /gene="rsmE"
                     /locus_tag="ON345_002973"
     CDS             2901..3632
                     /gene="rsmE"
                     /locus_tag="ON345_002973"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR3073003.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3645..4595
                     /gene="gshB"
                     /locus_tag="ON345_002974"
     CDS             3645..4595
                     /gene="gshB"
                     /locus_tag="ON345_002974"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR3073004.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4704..5267
                     /locus_tag="ON345_002975"
     CDS             4704..5267
                     /locus_tag="ON345_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR3073005.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5267..5683
                     /gene="ruvX"
                     /locus_tag="ON345_002976"
     CDS             5267..5683
                     /gene="ruvX"
                     /locus_tag="ON345_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR3073006.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5872..6852)
                     /locus_tag="ON345_002977"
     CDS             complement(5872..6852)
                     /locus_tag="ON345_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR3073007.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            6870..7574
                     /gene="yggS"
                     /locus_tag="ON345_002978"
     CDS             6870..7574
                     /gene="yggS"
                     /locus_tag="ON345_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR3073008.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7592..8158
                     /gene="yggT"
                     /locus_tag="ON345_002979"
     CDS             7592..8158
                     /gene="yggT"
                     /locus_tag="ON345_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR3073009.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8155..8445
                     /locus_tag="ON345_002980"
     CDS             8155..8445
                     /locus_tag="ON345_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR3073010.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8453..9046
                     /locus_tag="ON345_002981"
     CDS             8453..9046
                     /locus_tag="ON345_002981"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR3073011.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9039..10175
                     /gene="hemW"
                     /locus_tag="ON345_002982"
     CDS             9039..10175
                     /gene="hemW"
                     /locus_tag="ON345_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR3073012.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2387 a         2707 c         2712 g         2425 t
ORIGIN      
        1 tactcaccta tcttaattca caataaaaaa taaccatatt ggagggcatc atgcctgacg
       61 ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc cttgccgctc
      121 tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg ccgtttattg
      181 cagatgaatt ccagattact tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg
      241 gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt taaactcggg cgcaaaaaga
      301 gcctgatgat cggcgcaatt ttgtttgttg ccggttcgct gttctctgcg gctgcgccaa
      361 acgttgaagt actgattctt tcccgcgttc tactggggct ggcggtgggt gtggcctctt
      421 ataccgcgcc gctgtacctc tctgaaattg cgccggaaaa aattcgtggc agtatgatct
      481 cgatgtatca gttgatgatc actatcggga tcctcggtgc ttatctttct gataccgcct
      541 tcagctacac cggtgcatgg cactggatgc tgggtgtgat tatcatcccg gcaattttgc
      601 tgctgattgg tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc aaacgccgtt
      661 ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg
      721 aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg ctgtttaaag
      781 agaacagcaa cttccgccgc gcggtgttcc ttggcgtact gttgcagata atgcagcaat
      841 tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg gcgggttata
      901 ccaacactac cgagcaaatg tgggggaccg tgattgtcgg cctgaccaac gtacttgcca
      961 cctttatcgc aatcggcctt gttgaccgct ggggacgtaa accaacgcta acgctgggct
     1021 tcctggtgat ggctgctggc atgggcgtac tcggtacaat gatgcatatc ggtattcact
     1081 ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc ggttttgcca
     1141 tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg aaaggccgcg
     1201 attttggcat cacctgctcc actgccacca actggattgc caacatgatc gttggcgcaa
     1261 ccttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg tatgcggctc
     1321 tgaacgtact gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa cacgtttcgc
     1381 tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg
     1441 attaatctcc tcaggcttcc tcccgtcgcg gaggaagcca cctcttgcag tcatcttttc
     1501 ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat ccaacaggcc
     1561 gttatgcgtc gcctgcggga aaaactcgcc caggccaacc tgaagctagg gcgtaactac
     1621 ccggagccaa aactctctta cacccagcgc ggaacctccg ccggaacggc ctggctggaa
     1681 agctatgaaa ttcgcctcaa tcccgttttg ctgttggaaa acagtgaagc ttttattgaa
     1741 gaagtggtac cgcacgaact ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg
     1801 ccacatggca aagagtggaa gtggatgatg gaaagcgtgc tgggtgttcc cgcccgtcgt
     1861 acgcatcagt tcgaactgca atccgtgcgt cgcaacacct tcccctaccg ctgcaagtgc
     1921 caggagcatc agcttaccgt acgccgccat aatcgcgtag ttcgtggcga ggccgtctat
     1981 cgctgtgttc actgcggtga acagctggtt gcgaaataac catctgaact atcaggaact
     2041 ttcctgatct ggctgattgc ataccaaaac aactttcgct acgttgctgg ctcgttttaa
     2101 cacggagtaa gtgatgtacc gttatttgtc tattgctgcg gtggtactga gcgcagcatt
     2161 ttccggcccg gctttggccg aaggtatcaa tagtttttct caggcgaaag ccgcggcggt
     2221 aaaagtccac gctgacgcgc ccggtacgtt ttattgcgga tgtaaaatta actggcaggg
     2281 caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag gtgcgcaaaa atgaaaaccg
     2341 cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg cagttcggtc accagcgcca
     2401 gtgctggcag gacggtggac gtaaaaactg cgctaaagat ccggtctatc gcaagatgga
     2461 aagcgatatg cataacctgc aaccgtcagt cggcgaggtg aatggcgatc gcggcaactt
     2521 tatgtacagc cagtggaatg gcggcgaagg ccagtacggt caatgcgcca tgaaggtcga
     2581 tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc ggtgccattg cgcgcaccta
     2641 cttctatatg cgcgaccaat acaacctgac actctctcgc cagcaaacgc agctgttcaa
     2701 cgcatggaac aagatgtatc cggttaccga ctgggagtgc gagcgcgatg aacgcatcgc
     2761 gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct tgccaggcgc gaaagagcta
     2821 acctacacta gcgggattct ttttgttaac ccctacccca cgcgtacaac cgcgtgggga
     2881 gacgacgcgg atttttaact atgcgtatcc cccgcattta tcatcctgaa ccactgacca
     2941 gccattctca catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc gtactgcgca
     3001 tggggccggg gcaggcgttg caattgtttg acggtagcaa ccaggtcttt gacgccgaaa
     3061 ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct ggaaggcaag atcgacgatc
     3121 gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat
     3181 ttacgatcca gaaatcgatc gaactcggtg taagcctcat tacgccactt ttttctgagc
     3241 gctgcggcgt taaactggat agtgaacgtc tgaacaagaa gcttcagcag tggcagaaga
     3301 ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt gccggaaatc cgtccagcga
     3361 tggatctgga agcctggtgt gcagagcagg atgaaggact gaaactgaat cttcacccgc
     3421 gcgccagtaa cagcatcaat acgttgcctt taccggttga acgcgtccgc ctgctgattg
     3481 gcccggaagg cggtttatcg gcagatgaaa ttgccatgac tgcccgctat caatttactg
     3541 atatcctgtt gggacctcgc gttttgcgta cagagacaac tgcgctcacc gccattaccg
     3601 cgctacaagt acgatttggc gatttgggct aacggagaag aataatgatc aagctcggca
     3661 tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga ttccagtttc gctatgttgc
     3721 tggaagcaca gcgtcgtggt tacgaacttc actatatgga gatgggcgat ctgtatctga
     3781 tcaatggcga agcccgcgcc catacccgca cgctgaacgt gaagcagaac tacgaagagt
     3841 ggttttcatt cgtcggtgaa caggatctgc cgctggccga tctcgatgtg atcctgatgc
     3901 gtaaagaccc gccgtttgat accgagttta tctacgcgac ctatattctg gaacgtgccg
     3961 aagagaaagg gacgctgatc gttaacaagc cgcagagcct gcgcgactgt aacgagaaac
     4021 tgtttaccgc ctggttctct gacttaacgc cagaaacgct ggttacgcgc aataaagcgc
     4081 agctaaaagc gttctgggag aaacacagcg acatcattct taagccgctg gacggtatgg
     4141 gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa cctcggcgtg attgccgaaa
     4201 ccctgactga gcatggcact cgctactgca tggcgcaaaa ttacctgcca gccattaaag
     4261 atggcgacaa acgcgtgctg gtggtggatg gcgagccggt accgtactgc ctggcgcgta
     4321 ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc
     4381 cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg gccgacgctg aaagaaaaag
     4441 ggctgatttt tgttggtctg gatatcatcg gcgaccgtct gactgaaatt aacgtcacca
     4501 gcccaacctg tattcgtgag attgaagcag agtttccggt gtcgatcacc ggaatgttaa
     4561 tggatgccat cgaagcacgt ttacagcagc agtaacccac cttagcgaga aggatctcgt
     4621 tgagactctg agtgacagcg cccttctttc cacgcatact gggcgctgtt gcttttttga
     4681 accaggaaac agaacctctg acaatgaatt tacagcatca ctttcttatt gccatgcctg
     4741 ctctccagga tccgattttc cgtcgttccg tggtctacat ttgcgaacat aataccaatg
     4801 gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct caaaattgaa gggattctgg
     4861 aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat ccgtctggat aaaccggtta
     4921 tgctcggcgg tccgctggct gaagatcgcg ggtttatttt gcatactccg ccctccaatt
     4981 ttgcttccag cattcgcatt tcagacaaca cagtgatgac cacttcccgc gatgtgctgg
     5041 aaacgctcgg caccgataaa caaccgtctg acgtattggt ggctctgggt tatgcctcct
     5101 gggaaaaagg tcaactggaa caagaaattc tcgataacgc gtggctaacg gccccggcgg
     5161 atctgaatat tctgttcaaa acgccgattg ccgaccgctg gcgcgaggcg gcaaaactga
     5221 ttggtgtgga tattctcacc atgcctggtg tggcagggca cgcctgatga gtggaacctt
     5281 actcgccttc gacttcggca ccaaaagcat tggcgtagcg gtcggccaac gcattaccgg
     5341 cactgctcgc cctttgcctg caattaaagc acaggacggt acgccggact ggaacctcat
     5401 cgaacgttta ctgaaagagt ggcagccgga cgaaatcatc gtcggtttgc cgctgaatat
     5461 ggacggcacc gagcaaccat tgactgccag agcgcgtaaa tttgccaacc gtattcatgg
     5521 tcgtttcggt gttgaagtaa agctccatga cgaacgtctt agcaccgtgg aagcccgttc
     5581 cggtctgttt gaacagggcg gctatcgggc gctcaacaaa ggcaaagttg actctgcctc
     5641 tgcggttatt attctcgaaa gctatttcga gcagggatat taaggcgatt taaacgcctg
     5701 ctggagtgta taatatcatc gcgccttacg ccttattgcc ggatgcggcg tgaacgcctt
     5761 atccggccta catgtggcag cgattgtagg tctgataaga cgcgttagcg tcgcatcaga
     5821 catttattgc ccttataggc cctataaact tgcgcatcgg gcatggcaac gtcacaaacg
     5881 cccttccccc acccgctgct gataactctg ctgaaacgtt atcatcccca cctgctgccc
     5941 ggtttgaata acatgcggta actggtgggt tttcccttcg cgaatcaaat tccccaccgc
     6001 gggagtgtta atcaataatt caaacagcgc cacgcgtcct tcctgtttat ccacttccag
     6061 cttttgtgac aacacggccc gtaaactacc tgccagttga ttacgtacgg gatctttttc
     6121 ctgcgccgga aatgaatcca ccagtcgctc aactgcctgc gcggcaccac gcgtatgtaa
     6181 tgttgccagc accaaatgcc cggtttctgc cgccgtcagt gccagacgga ttgtctcgct
     6241 gtcacgcagc tctccgagca aaatcacatc aggatcttcc cgcaatgcgg cccgcaatcc
     6301 cgatgcgaac gtcatacagt gcaaaccaat ttcccgctgc tggatcaaac atcgctggct
     6361 ggcatagata tattcaacag gatcttccag cgtcagaata tgcgcatcgg catgttgatt
     6421 gagatagcca accatcgccg ccagcgtggt agatttgccg ctccccgtcg cccccgtcac
     6481 cagaatcagg ccattctcgc tcttgagtaa ttccggcaat accgttggtg cgccaagctg
     6541 ttcgagctgt ggacagtgcg aaggtaacaa ccgtaatgcc agagaaatac cttgccgttg
     6601 cgcgaacgca ctgccacgca accgctggtt ttccgccagc gacacggcaa aatccagctg
     6661 gccattttcc agcaatattg cccgctgatc gtcatccagc cactcccgca gtagctcttc
     6721 gacgtccggc gcatcaaacg gcgcagcttc cattctgcca cgaatgcgcc atcgtgcggg
     6781 ccaggcgctg cacaggtgta gatccgagac gttatgcttt acactaaggg ccacaatttc
     6841 ttccatattc atactaagat cctcggaaaa tgaacgatat tgcgcataac ctggcacagg
     6901 tccgggacaa aatctcagcg gctgcaacgc gttgcggccg ttctccagaa gaaattacgc
     6961 tgcttgcagt cagtaaaaca aaacctgcga gcgccatcgc agaagccatt gatgccgggc
     7021 agcgtcaatt tggtgaaaac tacgttcagg aaggggtaga taaaattcgc cactttcagg
     7081 aactgggcgt aacaggatta gaatggcatt ttattggccc gttgcagtct aataaaagcc
     7141 gcctggtggc agagcatttc gactggtgtc ataccatcga ccgtttgcgc attgctaccc
     7201 gcctcaacga tcagcgcccg gcagaacttc cccctcttaa cgtactgatt caaattaata
     7261 ttagtgatga aaacagtaag tccgggattc aactggcaga actggacgag ctggcagcgg
     7321 cggtcgctga actaccgcgt ttacgtctgc gcgggctgat ggcaatccct gcgcctgagt
     7381 cagaatatgt aaggcagttt gaagttgcac gccaaatggc tgtagcattt gccggactga
     7441 aaacgcgcta cccgcatatc gacacgctct ctctgggaat gtcggacgat atggaagccg
     7501 ccattgcggc aggtagcacg atggttcgta tcggcactgc aatttttggt gcgcgtgatt
     7561 actctaaaaa ataaggaatt aaaggaacgc catgaatacg ttgactttcc tgctttcaac
     7621 ggtcattgag ctgtatacca tggtgctgtt attacgcatc tggatgcagt gggctcattg
     7681 tgatttttac aaccccttct cacagtttgt agtgaaggta acacagccaa ttatcgggcc
     7741 actgcgccgc gttattccgg caatgggtcc cattgatagc gcctcgctgc tggttgcgta
     7801 cattctcagt tttatcaaag ccatcgtgct gtttaaagtg gtgaccttcc tgccaatcat
     7861 ctggattgcc ggtttactga ttctgctgaa aaccatcggc ctgctgattt tctgggtcct
     7921 gctggtgatg gcgattatga gctgggtaag ccagggtcgt agtccgattg aatacgtgct
     7981 gattcagctg gccgatccgc tgctgcgccc gattcgccgc ctgctaccgg caatgggtgg
     8041 gattgatttc tcgccgatga tcctcgttct gctgctgtat gtcatcaata tgggtgtcgc
     8101 agaagtatta caggcaaccg gaaacatgct gctgccgggg ctgtggatgg cgttatgagt
     8161 gccgtaacag ttaatgatga cggtctggtt ttacggctct atattcagcc gaaagccagc
     8221 cgtgattcta ttgtcggttt acatggcgac gaagttaaag tcgccattac cgcgccgccg
     8281 gttgacggcc aggccaacag ccatctggtg aagtttctcg gtaagcaatt ccgggtagcc
     8341 aaaagccagg tggtaattga aaaaggcgaa cttggccgcc acaaacaaat taaaatcatt
     8401 aatccgcaac aaatcccgcc agaaatcgcg gcgttaatta attaggtatc ctatgcaaaa
     8461 agttgtcctc gcaaccggca atgccggtaa agtgcgtgag ctggcgtcgc tgcttagcga
     8521 cttcggtctt gatatcgtgg cccaaacaga cctcggcgta gattccgctg aagaaaccgg
     8581 cctgaccttt atcgaaaacg cgattctgaa agcgcgccat gcggcaaaag tgaccggttt
     8641 accggcaatt gccgacgact ctggtctggc ggtagatgcg ctgggtggcg cgccgggtat
     8701 ttactccgcg cgttattccg gtgaagacgc gaccgatcaa aagaatctgc aaaaactgtt
     8761 ggaaacactg aaagacgtac cggacgacca acgtcaggcg cgtttccact gcgtgctggt
     8821 atatctgcgt cacgcggaag atccgactcc gctggtgtgc cacggtagct ggccgggcgt
     8881 gattactcgt gaaccagcgg gcactggtgg ctttggttat gatccaatct tcttcgtacc
     8941 ttccgaaggg aaaaccgctg ccgaactgac ccgcgaagaa aagagcgcca tttcccaccg
     9001 tggtcaggca ttgaaactgc tgttggacgc tttacgtaat ggttaaatta ccgccgctga
     9061 gtctctacat tcacatcccg tggtgcgtgc agaaatgccc gtactgcgat ttcaactctc
     9121 acgcgttgaa aggagaagtg ccgcacgacg attacgttca gcatctgctt tgcgatctgg
     9181 acaacgatgt ggcttacgct cagggccgtg aagtaaagac aatctttatt ggcggtggta
     9241 cgccgagcct gctttccggc ccggcgatgc aaacgctgct ggacggcgtg cgtgcgcgtt
     9301 tgccgctggc agcggatgca gaaattacta tggaagcgaa ccctggtacg gtagaagccg
     9361 atcgctttgt cgattatcag cgtgctggtg tgaaccgcat ctctattggt gtgcagagtt
     9421 ttagcgaaga caagctgaaa cgacttgggc gtattcatgg cccgcaagaa gcgaaacgcg
     9481 cggcaaagct ggcgagcggg ctggggctgc gtagctttaa cctcgatttg atgcatggac
     9541 taccggatca atcactggaa gaggcgcttg gcgatctacg ccaggccatt gaactgaatc
     9601 cgccgcatct ttcctggtat caactgacca tcgaacctaa tacgctattt ggttcacgcc
     9661 cgccggtact gccggacgac gacgcgctgt gggatatatt cgaacagggg catcagttat
     9721 taaccgcagc gggttatcag caatatgaaa cttccgctta cgccaaaccc agttatcagt
     9781 gccagcacaa tctcaactac tggcgctttg gcgactacat cggtattggc tgcggcgcgc
     9841 acggcaaagt gaccttcccg gatgggcgca ttctgcgtac cactaaaacg cgtcatccgc
     9901 gtggttttat gcaaggaagg tatctggaaa gccagcgtga tgtcgaagcc gcagataagc
     9961 cgtttgagtt ctttatgaat cgcttccgtc tgctggaggc cgcgccgcgc gtggagttta
    10021 ttgcgtatac cgggctttgc gaagatgtga ttcgcccaca gttagacgag gcgattgccc
    10081 agggttatct caccgaatgt gcggattact ggcagataac ggaacatggt aaattattct
    10141 taaactcact tcttgaatta ttcctcgctg agtaatcatt aatgcgtctg cgggcgcatt
    10201 aatcacttct tccccccccg ttaattaact t
//
LOCUS       DAJSKJ010000139        10110 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000139 DAJSKJ010000000
VERSION     DAJSKJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.140"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..651)
                     /locus_tag="ON345_002983"
     CDS             complement(<1..651)
                     /locus_tag="ON345_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3073013.1"
                     /translation="MSGFFLDLTKLSSGVNSDTAIPPRDIFTALPSKDAKFQYPRDVQ
                     SEVWEKWYDARKNQTNVIKMNTGSGKTSVGLIILKSCINEGEGPAVYAVPDNYLVEQV
                     ILEASQLGIPVTQDERSPKFIAGKEILVTNIFKLVNGRSVFGVGDEAPKIPIGAIIID
                     DAHACLSSIEDQFSINIQRNNPAYDRLFRIVENSLLTQASAKTLEIKSGDRNAYVRV"
     gene            complement(1017..2045)
                     /locus_tag="ON345_002984"
     CDS             complement(1017..2045)
                     /locus_tag="ON345_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3073014.1"
                     /translation="MKKSQKKQPKQATSMTASASQKMEAFTFGEPSPVLDRRDILDYV
                     ECINNGKWYEPPVNFSGLAKSLRAAVHHSSPIYVKRNILTSTYIPHPLLSRQDFSRLV
                     LDYLVFANGYLEKRMSVTGQLFKLETSPAKYTRRGVEDDVYWYVSSFTHPHQFAPGSV
                     FHLLEPDINQELYGMPEYLSALNSAWLNESATLFRRKYYQNGAHAGYIMYVTDAAQSS
                     TDVESLRSAMRDSKGLGNFKNLFFYAPNGKPDGIKIVPLSEVATKDDFFNIKKVSAAD
                     LLDAHRVPFQLMGGKPENIGSMGDIEKVARVFVRNELSPLQERFKEINEWLGMEVIRF
                     KDYSIDAD"
     gene            complement(2047..3816)
                     /locus_tag="ON345_002985"
     CDS             complement(2047..3816)
                     /locus_tag="ON345_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCR3073015.1"
                     /translation="MTISTDLSLLHDPRRQARLLFWQGYSVPQISETLQVKRPTVQSW
                     KQRDGWEETAPLNRVETTLEARLIQLYAKPDLTPHDFKIADFLSRQMERLARVNRYGQ
                     TGNEVDLNPRIANRNKGDRKKPKRNYFSEEAIEKLEEIFLDQSFEYQLNWHKAGLAHR
                     IRHILKSRQIGATFYFAREALLRALKTGQNQIFLSASKTQAYVFRKYIIAFARLVDVD
                     LSGDPIVIGNNGAELIFLGTNSNTAQSHNGDLYVDEIFWIPNFQRLRKVASGMASQSH
                     LRTTYFSTPSTLAHGAYPFWSGELFNRGRSNRDERVDIDISHQALAGGLLCPDGQWRQ
                     IVTIEDALAGGCTLFNLDQLKQENSADDFRNLFMCEFVDDKASVFPFEELQRCMVDAM
                     EEWEDFEPFADRPFNWRPVWIGYDPSHTGDSAGCAVLAPPLVAGGKFRILERHQWKGM
                     DFAAQAEAIRSLTEKYTVDYIGIDATGIGQGVYQLVRSFFPAARAIRYTPEMKTAMVL
                     KAKDTIRRGCLEYDAGATDITQSFMAIRKTMTSSGRSSTYEASRSEEASHADIAWATM
                     HALLNEPLSAGSGMHSTSILDIN"
     gene            3983..4837
                     /locus_tag="ON345_002986"
     CDS             3983..4837
                     /locus_tag="ON345_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCR3073016.1"
                     /translation="MAKKVSKWFRIGVEGDTCDGRVISGDDIQDMADTFDPRVYGCRI
                     NLEHLRGILPDSVLKRYGDVTAVKAEIISDDSALNGKKALFGKIQPLDELVSMVRAGQ
                     KVYTSMEIRPNFANSGKCYLVGLAVTDDPASLGTEYLEFCGRASQNPLAGKKAHPDDV
                     FSVASLATLEFEDVPDTMLNSLTDAVKAIFSRKQSTDDARFDDVHEAVTTVSEQVQTN
                     LTENDKRVTALETAFAQLKQDVTSKAEENAQAFSSLKSSLDNTESLSQPRREKSKGGT
                     GDELLTNC"
     gene            4896..5990
                     /locus_tag="ON345_002987"
     CDS             4896..5990
                     /locus_tag="ON345_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCR3073017.1"
                     /translation="MRKDTRFKFNAYLSRVAELNGVSTDDVAKKFTVEPSVTQTLMNT
                     LQMSSAFLTKINIVPVDELKGEKVGVGVNGTIASTTDTAGDDERKTADFTALESNKYE
                     CAQINFDFHIRYKQLDLWARFQDFQTRIRDAIIKRQSLDFIMAGFNGIERADTSNRKN
                     NPMLQDVAVGWLQKYRDEAAARVMSNITDEDGKVISDVIRVGKNGDYENLDALVMDAT
                     GNLIDEIYQDDPELVVITGRKLMADKYFPLVNKAQENSETLAADIIISQKRIGNLPAV
                     RVPYFPANALMVTRLDNLSIYFMDDAHRRAIIEEPKKDRIENYESMNIDYVVEAYAAG
                     CLIENINLGDFTPAEAPQSAAAPVEQEDGE"
     gene            5994..6764
                     /locus_tag="ON345_002988"
     CDS             5994..6764
                     /locus_tag="ON345_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR3073018.1"
                     /translation="MKSPAARHMMRVSASETAQRAATPLRNATAYEQMLVKVAADNRT
                     LKQIRSKEHKAAKKRELLPFYLPWVAGVLSGGKGAQDDIVMTVMLWRLDANDIAGALE
                     IARYAMTYGLTMPTGRRPTPYLLAEEVALAAQRLRDAKQPVELANLLDTLDLTARADM
                     PDIVRAKLHKITGYVLRNAGQQADALAHLQRAIQLEGAIGVRKDIEQLERALKPKPEP
                     APKAGAAPKVNKPRTRKATAKPAARRGRPPKAATKAAG"
     gene            6864..7370
                     /locus_tag="ON345_002989"
     CDS             6864..7370
                     /locus_tag="ON345_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR3073019.1"
                     /translation="MTTLIIEQNKEPQDVPGVVIPPPGVSEPVIKNTPFYPDVDPKRV
                     REEMRLEQTVSPVRLRRAIKTAIAETNAELGEWRELQLDAGYATLADVPTDELDGESV
                     RVFHYFNAVCSMTTATLYERYRGVDATGKGDKKADSIDSTIDEMWRDMRWSVARIQDK
                     ARCIVGQI"
     gene            7370..7573
                     /locus_tag="ON345_002990"
     CDS             7370..7573
                     /locus_tag="ON345_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR3073020.1"
                     /translation="MRAYALQGDTLDAICARYYGRTEGVVETVLEANPGLSELGVVLP
                     HGTAIELPEADSAARAETVSLWD"
     gene            7564..7785
                     /locus_tag="ON345_002991"
     CDS             7564..7785
                     /locus_tag="ON345_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HCR3073021.1"
                     /translation="MGLSMEKITTVIAYWLAVGLAYFGAMSPEKVALYVGGGCAIFTA
                     LTNYWFKRKTYRYLTSLGLDKGAIREINH"
     gene            7769..8278
                     /locus_tag="ON345_002992"
     CDS             7769..8278
                     /locus_tag="ON345_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR3073022.1"
                     /translation="MKSIIKKCSVAAVLTLAALMPDFRLLNTSPEGLALLADLEGCRL
                     TPYQCSAGVWTSGIGHTAGVVPKGDITERQAAANLVADVLNVEQRLAVCVPVEMPPHI
                     YDTLVSFAFNVGTGAACRSTLVSFIKRQQWWQACDQLTRWVFVNGVRNKGLENRRTRE
                     RAYCIRGLQ"
     gene            8275..8703
                     /locus_tag="ON345_002993"
     CDS             8275..8703
                     /locus_tag="ON345_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCR3073023.1"
                     /translation="MKIAVCFVLAALVAVVLWLRHENGNLTRSFERANKVAGEQKNVI
                     EMLKNQLSVSQRIARTNEAAQVRLGNELLTAGVMAAEREETIARLMSENETLRQWSGD
                     KLPDVVRRLHTRTGCASAAHCLQRLPESEPLPDAGERAHH"
     gene            8799..9266
                     /locus_tag="ON345_002994"
     CDS             8799..9266
                     /locus_tag="ON345_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3073024.1"
                     /translation="MKKAQSLRKALTDAVPQLKTNPEMMRIFADEGNIDARLAASLSH
                     EKIYTLNVIVCDFVGDPDLIFVPVAAWLRENQPDICTLDEGRKKGYRFQMDLNDGDNV
                     DISISLQLTERTIIKEENGALHVSYAPEPPLPEPVTPPKELYINGELVSKWDE"
     gene            9259..9705
                     /locus_tag="ON345_002995"
     CDS             9259..9705
                     /locus_tag="ON345_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR3073025.1"
                     /translation="MSDLKPFDDRLAGLIASLSPAGRRKLAGEIAKELRTAQQQRIKQ
                     QKAPDGSPYQARKRQPLRAKTGRIKRAMFQKLRTSRYMKASGRENRAVVEFTGKVQRI
                     AQIHQYGLKDRPSSYAKSVQYPKRILIGYSERDETVVMKLIMDALS"
     gene            9875..>10110
                     /locus_tag="ON345_002996"
     CDS             9875..>10110
                     /locus_tag="ON345_002996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073026.1"
                     /translation="MLDAKKLIHSSKDYFVLVGKNGSGKSRMLHDLAESIHDYGYSTI
                     VVSNTLFDKFEIHPQSDNYNYIGSKLGRNFPAQA"
BASE COUNT         2354 a         2651 c         2859 g         2246 t
ORIGIN      
        1 aactcttaca tatgcgtttc tatcgcctga ttttatttca agtgtctttg cactagcttg
       61 tgtcagtaac gagttttcaa ctattctgaa tagcctatcg taagcgggat tgtttcgctg
      121 tatgttgata gagaattggt cttcgattga tgaaagacag gcgtgagcat catctattat
      181 tatggcgcct attgggattt ttggtgcttc atctccaacg ccaaatactg aacgaccatt
      241 aaccagctta aaaatgtttg tgactaaaat ctctttgcct gcaatgaatt tgggggatct
      301 ctcgtcctgg gttactggta ttccgagttg actagcttcc aagataactt gttcaaccag
      361 atagttgtca gggaccgcat atacagctgg cccttcccct tcatttatac agctttttaa
      421 tattattaac ccgactgatg ttttaccgct tccggtattc atctttatta cattggtttg
      481 attttttctt gcatcatacc atttttccca aacttcagac tgtacgtctc gggggtattg
      541 aaattttgca tctttgcttg ggagtgctgt gaatatatca cggggtggga ttgctgtgtc
      601 actatttaca cccgaagaaa gttttgttaa atcaaggaaa aagccagaca ttttcaatct
      661 ccttattatg ctttagatta gttgttagca tcgaatgatc actatcacaa cttgcattag
      721 attgcattag attgcattca taaatgttac ttgtgtatga agttcgatga ttgatgggct
      781 gtggctttcc tcaactgcaa tattgcatca aaaccgaccc ataaagcggg caggcgaggc
      841 ggggatagca ctgcgcgcca gaggtggtga cagcatttaa tttagcgcgt ctgcacgcgt
      901 tgtgatgatg ctgctgtttc gctgattggt gcttttattc ttgtgtgttt gcggcgtatg
      961 aagagcgtct gaggctttct gagaggatgc cgcccggagg cggcatttcg gcggggttag
     1021 tcggcgtcga tgctgtaatc tttaaagcgg atgacctcca ttcccagcca ttcgtttatc
     1081 tctttaaagc gctcctgcag tggcgacagt tcgttacgca caaatacccg cgccaccttc
     1141 tcgatatcac ccattgagcc gatattctca ggcttgcccc ccatcagctg gaacggcacg
     1201 cggtgcgcgt cgagcaggtc ggcggcgctg acctttttga tattgaaaaa atcatccttt
     1261 gtggcgactt cactcaacgg cacaattttg atgccgtccg gtttgccgtt gggggcgtaa
     1321 aaaaacaggt ttttgaaatt gccgagccct ttcgaatcac gcattgcaga gcgaagcgac
     1381 tcgacgtcgg tgctgctttg cgccgcgtcg gtcacgtaca tgatgtaacc cgcgtgcgcg
     1441 ccgttctggt aatacttgcg gcgaaacagc gtcgcggact cattcagcca ggcggaatta
     1501 agcgcgctca ggtattccgg catcccgtaa agctcctgat tgatgtcagg ctcaagcaga
     1561 tggaacaccg agccgggggc gaactggtgc gggtgggtaa agctcgatac gtaccagtag
     1621 acatcatcct ccacaccccg gcgggtgtat ttggccgggg aggtttcaag cttaaagagc
     1681 tgtccggtca cgctcatgcg cttttccaga tagccattcg caaagaccag ataatcaagc
     1741 acaaggcggc tgaagtcctg acgcgacaac aacgggtgcg ggatgtaggt gcttgtaagg
     1801 atgttgcgct tcacgtaaat cggggagctg tgatgtacgg cggcgcgcag gcttttcgcc
     1861 agccccgaga agttgaccgg cggctcgtac cacttgccgt tattaatgca ctcaacgtaa
     1921 tcgagaatgt cgcggcgatc cagaacgggg gagggctcac caaaggtgaa cgcctccatt
     1981 ttctgcgatg cgctggcggt catgctggtt gcctgtttcg gctgcttttt ttggcttttt
     2041 ttcatcttag ttaatatcca gaattgatgt tgagtgcata ccgctaccgg ctgatagcgg
     2101 ttcgtttaac agggcgtgca tggttgccca ggcgatatcc gcatggctgg cttcttcact
     2161 gcggctggcc tcataggttg agctcctgcc gctgctggtc atggttttgc ggatagccat
     2221 aaatgactgc gtgatgtcgg ttgccccggc gtcatattcc agacacccgc ggcgaatggt
     2281 gtctttcgct ttcagcacca tcgcggtttt catttccggc gtgtagcgga tggcgcgcgc
     2341 cgccgggaag aatgagcgca cgagctggta aacaccctgg ccgatgccgg tcgcatcaat
     2401 gccgatatag tcgacggtgt atttttcagt aagcgatcgg attgcctcgg cctgcgcggc
     2461 aaagtccatg cctttccact ggtggcgctc aaggatgcgg aacttgccac cggcaaccag
     2521 tggcggagcc agtaccgcac agcctgcgct gtcgccggtg tgtgacgggt cgtaaccaat
     2581 ccagaccggg cgccagttga acggacgatc ggcgaacggc tcgaagtcct cccactcttc
     2641 catcgcatcg accatgcagc gctgcagctc ctcgaacggg aataccgacg ccttatcgtc
     2701 gacgaactcg cacataaaca gattgcggaa atcgtcggcg ctgttttcct gtttaagctg
     2761 gtcgaggtta aacagggtgc agcccccggc gagcgcgtcc tcaatggtga caatctgccg
     2821 ccactggccg tccgggcaaa gcaagccacc ggccagcgcc tgatggctga tatcgatgtc
     2881 gacacgttcg tcacggttac tgcgcccccg gttaaacagc tcacctgacc agaacggata
     2941 agcaccgtgc gccagcgtgg agggggtcga aaaataggtt gtgcgcaggt gagactgtga
     3001 ggccataccc gaggctactt tgcgcagccg ctggaaattg ggaatccaga aaatttcatc
     3061 gacatacagg tcgccgttgt ggctctgcgc cgtgttggag ttggtgccga ggaaaatcag
     3121 ctctgcgccg ttgttgccga tgacaatcgg gtcgcctgac aggtcaacgt caaccagacg
     3181 ggcaaaggcg atgatgtact tacggaatac gtaagcctgc gttttactgg ctgagaggaa
     3241 tatctgattc tggccggtct tcagggcgcg cagaagagcc tcgcgcgcaa agtagaacgt
     3301 tgccccaatc tgacgcgatt tcagaatgtg gcggatacgg tgcgcaagcc cggccttatg
     3361 ccagttgagc tgatactcaa atgactggtc gaggaaaatc tcttccagct tctcgatagc
     3421 ctcctcgctg aaataattac gtttcggctt tttgcgatcc cccttgttgc ggttggcgat
     3481 tctgggattt aaatccacct cgttcccggt ctgaccgtag cggttcacgc gcgcgaggcg
     3541 ctccatctgg cgcgacagga aatcagcgat cttgaaatcg tgaggcgtca ggtctggttt
     3601 cgcgtagagc tgaatcagac gcgcctctaa cgttgtctcg actcggttga gcggggcggt
     3661 ttcctcccat ccatcgcgct gcttccagct ttgtacggtt ggacgcttaa cctgaagtgt
     3721 ttcggatatt tgcggtaccg aataaccctg ccagaacaaa agacgcgcct gtcgtcgtgg
     3781 atcatgcaga agagaaaggt cagtcgagat ggtcatgatt gcctcgagtt gatgaatacg
     3841 aggcaaggct aaagaattga actgacgtta tcgctaagcc ctcgttgtgt caggggttgc
     3901 acttccgcaa ccggtggctg atgcgggttg gagtcgggaa actacacctg aaccgaataa
     3961 cccaacatca ggacactgaa caatggcaaa gaaagtttct aaatggtttc gcatcggcgt
     4021 cgagggcgac acttgcgatg gccgcgtcat cagcggcgat gatattcagg atatggccga
     4081 cacgttcgac ccgcgtgtct atggttgccg catcaacctt gagcacctgc gcggcatttt
     4141 gcccgacagc gtgcttaaac gttatgggga tgttacagcg gttaaggctg agattatcag
     4201 cgacgattcc gccctgaatg gcaaaaaagc gctgtttggc aaaatccagc cgctcgacga
     4261 gctagtcagc atggtgcgcg ccggtcagaa ggtttacacc tcgatggaga tccgcccgaa
     4321 ctttgccaat agcggcaagt gctacctcgt cgggctggcc gtgacagatg acccggcaag
     4381 cctcggtacc gagtatctgg aattctgcgg ccgcgccagc cagaacccgc tggccggtaa
     4441 aaaagcccac ccggatgacg ttttttctgt cgcttctctg gcaacgctgg aatttgaaga
     4501 cgttcccgac accatgctca acagcctgac cgacgcggtg aaagcgattt tcagccgtaa
     4561 acagtcaacc gacgatgcac gatttgacga tgtgcatgaa gcggtgacga ccgtcagcga
     4621 gcaggtgcaa accaacctca ccgaaaacga caagcgtgtc accgcgcttg agaccgcgtt
     4681 tgcacagctc aaacaggacg taaccagcaa agctgaagaa aacgcgcagg cgttcagctc
     4741 cctgaaaagc tctctcgata acaccgaaag cctgagccag ccgcgccgcg agaaatcgaa
     4801 gggcggcacc ggcgacgagc tgctgaccaa ctgctgataa cgcgccgggc gcgtggcgtc
     4861 cgggcatagc cattttttga attcaaggaa taacaatgcg taaagatacc cgctttaaat
     4921 ttaatgccta cctgagccgc gtagcggagc tgaacggcgt ttctactgac gacgtggcga
     4981 agaaattcac cgtcgagccg tcggtcacgc aaaccctgat gaacaccctg cagatgtcat
     5041 ccgcgtttct gaccaaaatc aacatcgtgc cggtcgatga gctgaagggc gagaaggtcg
     5101 gcgtgggcgt aaatggcacc atcgccagca ccacggatac tgccggtgat gacgaacgta
     5161 aaaccgccga ctttaccgcg ctggagtcca acaaatacga gtgcgcacag attaactttg
     5221 acttccatat ccgttacaag cagctcgacc tgtgggcgcg cttccaggac ttccagaccc
     5281 gtatccgtga cgcgattatc aagcgccagt cgctcgattt catcatggcc ggattcaacg
     5341 gtatcgagcg tgcggacacc tcaaaccgca aaaataaccc gatgcttcag gatgtggcgg
     5401 tgggctggct gcagaagtac cgcgatgagg ccgcagcgcg tgtgatgtca aacatcaccg
     5461 acgaagacgg caaggttatt tccgatgtga tccgcgtggg taaaaacggc gactacgaaa
     5521 accttgatgc gctggtcatg gacgccaccg gcaacctgat tgacgagatt tatcaggacg
     5581 atcccgagct tgtggttatc actggccgta agctgatggc cgacaagtat ttcccgctgg
     5641 tcaacaaggc gcaggaaaac agtgagacgc tggccgctga catcatcatc agtcagaaac
     5701 gcattggcaa cctgccagcc gtgcgcgtgc cgtacttccc ggcgaatgcc ctgatggtga
     5761 cgcgcctcga caacctgtcg atttacttca tggacgacgc gcatcgccgc gccatcatcg
     5821 aagagccgaa gaaagaccgc atcgaaaact acgagtcgat gaatatcgat tacgtggttg
     5881 aggcttacgc cgccggttgc ctgattgaaa acatcaatct cggtgatttc actcctgctg
     5941 aagcgccaca aagtgccgct gcaccggtag aacaggaaga cggagagtaa gccatgaaga
     6001 gccccgcagc gcgtcacatg atgcgggtct cggcctctga aacggcgcag cgggctgcaa
     6061 ctccgctgcg caatgcaact gcttacgagc agatgcttgt aaaagtggcc gcagacaacc
     6121 gcacgctgaa acaaatccgc tccaaagagc ataaggccgc taaaaagcgc gagctgctgc
     6181 cgttctatct gccgtgggtc gccggtgtgc tttccggtgg caagggtgcg caggatgaca
     6241 ttgtgatgac cgtcatgctg tggcgtctcg atgcaaacga catcgccggt gcgctggaga
     6301 ttgcccgtta tgccatgacc tacgggctca ccatgccgac cggtcgccgt ccgacgccgt
     6361 acctgctggc cgaagaggtt gcactcgcag cgcagcgcct gcgcgatgcg aaacagcccg
     6421 tcgaactggc gaaccttctc gatacgctcg acctcaccgc gcgtgcggat atgccggata
     6481 tcgtgcgcgc gaagctgcac aaaatcaccg gctacgtgct gcgtaatgcc gggcaacagg
     6541 ccgacgcgct ggcgcacctg cagcgcgcga tccagttaga gggcgcaatc ggcgtgcgta
     6601 aggatatcga gcaactggag cgagccctta aaccgaagcc tgaacccgca ccaaaagccg
     6661 gagcggctcc aaaagtgaat aaaccgcgca cgcgaaaggc caccgctaaa ccggcggcac
     6721 gtcgcgggcg tccaccaaaa gcggcaacga aagccgcagg ttaaacgagc gctccccgag
     6781 ccgggcggca cgccggtcaa tgcgggtatt cattgccctg actgcgaccg gcgtccaccg
     6841 cccacccatt acccgaggtt gtcatgacga cactgattat tgagcagaac aaagagccgc
     6901 aggatgtgcc gggcgtggtg attccgccac cgggcgtgag cgagccggta atcaaaaaca
     6961 caccgtttta cccggatgtt gatccgaagc gcgtgcgtga agagatgcgc cttgagcaga
     7021 ccgtttcccc tgtgcgcctg cgccgcgcga ttaaaaccgc catcgcggaa acaaacgccg
     7081 agctcggcga gtggcgcgag cttcagctcg atgccggtta cgccacgctg gcggatgtgc
     7141 cgaccgacga gctcgacggc gagagtgtgc gggtcttcca ctacttcaac gccgtgtgct
     7201 cgatgacgac cgccacgctt tatgagcggt atcgcggcgt ggatgcgacc ggcaaaggcg
     7261 acaaaaaggc cgacagtatc gacagcacta tcgatgaaat gtggcgtgat atgcgctggt
     7321 ctgtggcacg tattcaggac aaggcgcgct gcattgtggg gcaaatctga tgagggctta
     7381 tgcgctgcag ggcgacaccc tcgacgcgat ttgcgcccgg tattacggac gcactgaggg
     7441 ggtggtcgaa accgtgttag aggctaatcc cggtctgtct gaactcggtg tcgtcctgcc
     7501 acacggaacg gcaatcgaat tacccgaagc cgacagcgcg gccagagccg aaacggtgag
     7561 tttatgggat tgagtatgga aaaaatcacg acggttatcg cctactggct ggcagttggg
     7621 ctggcgtatt tcggggcgat gtcacccgaa aaggtggcgc tttatgtggg cggcgggtgc
     7681 gccattttta ccgcgctgac caattactgg tttaagcgca aaacgtaccg ctacctgacg
     7741 tcactcggcc ttgataaggg ggctattcgt gaaatcaatc attaaaaaat gcagtgtggc
     7801 cgcagtgctg acgctggcgg cgctgatgcc tgactttcgt ctgcttaaca cctcgccgga
     7861 ggggctggca ctactggccg acctcgaagg gtgtcgcctg acaccttacc agtgtagcgc
     7921 gggagtgtgg acgtcgggca tcggccacac tgcaggcgtg gtgccgaagg gggatatcac
     7981 cgagcgtcag gcggcggcga acctcgtcgc ggatgtgctg aacgtcgagc aaaggctcgc
     8041 ggtctgcgta ccggtggaaa tgcccccgca tatctatgac acccttgtca gctttgcctt
     8101 taacgtcggc accggcgcgg cctgtcgttc gacgctggtc tcgtttatca agcgtcagca
     8161 gtggtggcag gcgtgcgacc agctcacccg ctgggtattc gtcaatggcg tcaggaataa
     8221 aggacttgag aatcgtcgca cgcgtgagcg tgcctactgc atcagggggc ttcaatgaaa
     8281 atagcagtgt gttttgtgct ggccgcgctg gtcgccgtgg tgctgtggct gcgccacgaa
     8341 aacggcaacc tgacccgctc ctttgagcgc gcgaacaagg tcgccggtga acaaaaaaac
     8401 gtgatcgaga tgctgaaaaa tcagctttca gtatctcagc gaatcgccag gacaaacgaa
     8461 gccgcgcagg tgcggctcgg caacgagttg cttaccgcag gagtgatggc ggcggagcgt
     8521 gaagaaacca tagcgaggct aatgagtgaa aatgaaacgt tgcgccagtg gtctggcgat
     8581 aagcttcctg atgttgtgcg ccggttgcac acccgaacgg gctgcgcctc cgccgctcat
     8641 tgtttacaac gcctgcccga aagtgagcct ctgcccgatg ccggggagcg agcccaccac
     8701 taacggtgat ttgagtacgg atattcgcag gcttgaactg gcgctcactg cctgcgcgat
     8761 taaggtcgaa accatcaaag attgtcagga taaagtcgat gaagaaagca cagagcctgc
     8821 gaaaagcctt aactgacgct gtaccgcagt taaaaaccaa cccggaaatg atgcgtatct
     8881 ttgcggatga ggggaatatc gatgcgcgtc tcgcggcctc gctgtcacac gaaaagattt
     8941 ataccctgaa tgtgatcgtg tgtgattttg tcggcgaccc ggatttgatt ttcgttccgg
     9001 tggccgcgtg gctcagggaa aaccagccgg atatctgcac gctggatgag gggcgtaaga
     9061 aaggctatcg attccagatg gatttaaacg acggtgacaa cgttgatatc agtatcagcc
     9121 tgcagctcac cgagcgcacc atcatcaaag aggaaaacgg ggcgctgcat gtcagttatg
     9181 ccccggagcc gccattgcct gaacctgtta ccccgccgaa agagctgtat atcaacggcg
     9241 agctggtgag caagtgggat gagtgattta aaaccttttg acgatcggct cgccgggctg
     9301 attgcctccc tgtcacctgc agggcgtcgg aaactcgccg gtgaaatagc gaaagagctc
     9361 aggacggcgc aacagcaacg gattaaacag cagaaagccc cggacggctc accctatcag
     9421 gcgcgaaagc gtcagccgct cagggcaaag accgggcgaa tcaaaagggc gatgtttcag
     9481 aagctgcgca cgagccggta catgaaagcc agtggccgtg aaaaccgtgc agtagtggaa
     9541 tttaccggca aagtgcagcg catagcgcag atacatcagt acgggttaaa agatcgtcct
     9601 agctcgtacg ctaaaagtgt tcaataccct aagcgtattc taattggata tagtgaaaga
     9661 gatgaaacag tggttatgaa attaatcatg gatgcactaa gttaaatcct atgcgttggc
     9721 ggtatgtttt tattatggcc ttataaaaaa ggattttccc acaagcaata aaagatcttg
     9781 ttcaataaca tcgagttctt aagatttgag agttgccaac tcttgaggta gagatgtaga
     9841 attcaccatt ctggtgaatt gttagggatt ttatatgctt gatgcaaaaa aacttatcca
     9901 ctcgtcaaaa gattattttg ttttggtcgg aaaaaacgga agcggcaaaa gtcgtatgct
     9961 tcatgatttg gctgagtcaa ttcacgacta tggctatagc actattgtag tgtccaatac
    10021 tctctttgat aaatttgaaa ttcaccctca aagcgacaac tataattata taggtagtaa
    10081 gctagggcga aactttcctg cgcaagcgat
//
LOCUS       DAJSKJ010000140         9976 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000140 DAJSKJ010000000
VERSION     DAJSKJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.141"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..614)
                     /locus_tag="ON345_002997"
     CDS             complement(<1..614)
                     /locus_tag="ON345_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR3073027.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEI"
     gene            881..2380
                     /gene="ansP"
                     /locus_tag="ON345_002998"
     CDS             881..2380
                     /gene="ansP"
                     /locus_tag="ON345_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR3073028.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALAIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2493..3554)
                     /locus_tag="ON345_002999"
     CDS             complement(2493..3554)
                     /locus_tag="ON345_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR3073029.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3796..5898
                     /locus_tag="ON345_003000"
     CDS             3796..5898
                     /locus_tag="ON345_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR3073030.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5934..6599)
                     /gene="mcbR"
                     /locus_tag="ON345_003001"
                     /pseudo
     CDS             complement(5934..6599)
                     /gene="mcbR"
                     /locus_tag="ON345_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
     gene            complement(6797..7834)
                     /gene="curA"
                     /locus_tag="ON345_003002"
     CDS             complement(6797..7834)
                     /gene="curA"
                     /locus_tag="ON345_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR3073031.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWMLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFVIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8015..8533
                     /gene="mddA"
                     /locus_tag="ON345_003003"
     CDS             8015..8533
                     /gene="mddA"
                     /locus_tag="ON345_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR3073032.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8530..8979
                     /locus_tag="ON345_003004"
     CDS             8530..8979
                     /locus_tag="ON345_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR3073033.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVFFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIAGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8980..9213)
                     /locus_tag="ON345_003005"
     CDS             complement(8980..9213)
                     /locus_tag="ON345_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR3073034.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9299..9472)
                     /gene="ortT"
                     /locus_tag="ON345_003006"
     CDS             complement(9299..9472)
                     /gene="ortT"
                     /locus_tag="ON345_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR3073035.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9667..9762
                     /gene="yncL"
                     /locus_tag="ON345_003007"
     CDS             9667..9762
                     /gene="yncL"
                     /locus_tag="ON345_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR3073036.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2412 a         2440 c         2428 g         2696 t
ORIGIN      
        1 ataatttcat tcctttttaa tacttcttgt aatttcggta actgacatac agcatcagca
       61 atcgcactga tattcgtggc attatcctga aaccattcat gcccaggccc ccatgtgcgc
      121 atagtgcaaa gataacagtc caccagcgtt agttgctcac caaacgcata aggttcagcg
      181 gtcaattgtg aattcagcca gatataaagc gatttacgat attcaataac attcttcttt
      241 aactgttcag gtgcgtcagg agcccaacgc tcagggtaat cggcgaaagt gaatgttgga
      301 tagacattag caaccagcca gaccaatagc cgttgaaact gctgacgttc ggcgcgccca
      361 accggtggcg caaggtccgg gcgacgatcg agaaccatca atgcaatcgc cgctgtctcc
      421 gtcataatct cgtcattttc cagcgccagt gtcggaatct gacacaacgg gttcagggtt
      481 ttcaataact cgcgtgaagt tccctcgtgg tcaaaaccac tgacatcaac gaactgataa
      541 ggaatatcag ccagtgttag catcagctca ctgattgtag agccccatcc gggtacgcca
      601 tacaccttaa tcatatgccc ctcacaatct tttaaagatt aagtgtagga cagtacacag
      661 gttaaccagc aaaaggattt gtcatttttt cgtcagttgt attaatcccc ttcaggattg
      721 taaggggatg ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat
      781 acccataaag aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc
      841 aacttatatg actttttcat taaagcaatc agggagagca atgagtaaac acgacaccga
      901 cacttcagat caacacgccg cgaaacgccg ctggcttaat gcccacgaag aggggtatca
      961 caaagcgatg ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggcaccgg
     1021 cttgttttta ggtgcaggag cccgactgca aatggcgggt ccagcactgg cactggttta
     1081 tttaatttgt ggcttgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgctaca
     1141 ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaagccgc
     1201 ttatgttgct ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac
     1261 cgccgtcgct ctgtatatgc attactgggg tgcgtttggc ggcgtgccgc agtgggtctt
     1321 tgcgctcgct gcacttgcca tcgttggcac catgaatatg atcggtgtga aatggtttgc
     1381 ggagatggag ttctggtttg cgcttattaa agtgctcgcc attgtgacct ttttggtcgt
     1441 gggtacagtg ttcctcggta gtggtcagcc gctggatggc aacaccactg gctttcattt
     1501 aatcaccgat aatggcggct tcttccccca cggtttgctg cctgcgctgg tgttgattca
     1561 gggcgtagtg tttgcttttg cctccattga aatggtgggt acagctgccg gagaatgtaa
     1621 agatccgcag accatggtgc ctaaagccat taacagtgtg atttggcgta ttggcctgtt
     1681 ttacgtcggc tccgtggtgt tgctggttat gttattgccg tggagcgcgt atcaggcggg
     1741 gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg ccatatatcg gcagcattat
     1801 gaacattgtg gtgctgaccg ctgccctctc cagcctgaac tcaggtctgt actgcaccgg
     1861 acgtattctg cgctcaatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag
     1921 tcgtcagcat gtgccgtatg ccgggattct agcgacactg gttgtgtatg tcgtcggcgt
     1981 tttcctcaac tatctggtgc cgtcgcgtgt atttgagatt gtattgaact tcgcgtcgct
     2041 gggaatcatc gcttcatggg cgtttatcat cgtgtgccag atgcgcctgc gtaaagcgat
     2101 taaagaaggc aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg
     2161 gctgacatta ctgtttttac tgagtgtcct tgtgctgatg gcgttcgatt acccgaacgg
     2221 gacttatact atcgcggcgc tgccgattat cggtattctg ctggttatag gctggtttgg
     2281 tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga
     2341 aaaacaggaa attgtgttta agcctgaaac ggcgagttaa ttgcaaaatt gcccggctac
     2401 gtgccgggca aattcagatt actgacgaaa gtgccggata cgccgtgata aaaggcaggc
     2461 aagtctcacc gttttcaccc tcgccatatc tattacagcg caatacgaat cacatcgtcc
     2521 ggctgggtag cttcctgctg tttagtggat ttttgtttca cactgacata cagcgttttg
     2581 ccatcggcag acagcgccag gctgtttgga tgagtcggcg tatcgaacgt tttcaccact
     2641 ttatagcttt tcgcgtcaat cacactgact ttacctgcct gacgatgcgt tacgtaggct
     2701 tcattacgcg cggggttaaa cagcacagcc agtgattccg gtgccgcaac cttcgccaga
     2761 atattgccat tacgggtatc gaccactaac acttctgccg ctttagaatc ggtgataaat
     2821 gcacgctgcc tggcggtatc aaggctgatg ttgataaaga agtgctcttt gccgtcatcc
     2881 agcagctttt tacggctgag gattttattg tcggcggtgt cgatggtaat caattcgccg
     2941 tcagcgttag tggtgtaaag acgtttgcct ttgctatcaa gcgccagacc ggtactcatt
     3001 ttaccggtgt tctggatggc ggttttcagt ttgatattct cgccatcaac gacccaaatc
     3061 acgctctctt taccaatacc actgatgtaa acggtgttcg tggcatcatc agctaccagc
     3121 tcacgcggtt gcagcgggcg cacctcttcc gtgcgcttac gatcatccag caccagacgg
     3181 cctttcactt cgcccgtttt ggcatctatc gccgtgaccg cgctgtttac ggtgttacca
     3241 aaccacaacg tctgagtcgt gttattgatg gtggcaccaa acggcttgag atcgttatgg
     3301 atcgcctgcg tcacttccag agtgaccgga tcaagacgat aaaccacgcc acctttatcc
     3361 agtttgcggc tttgcgaagt ggcgagccac agcgcgtttt cttgctggct ataagccatt
     3421 tcgtaggcac ctttacctac cgctttacgc agcatttctt ctgcggcctg cgtactgaat
     3481 gatgaagcaa caagcaatga acctaacagt aatgaaccac gcaggcgcga tgaaaacaga
     3541 tgacgtaaat gcatgacgac tccctttgat gaaccaatat ttttgctgct tcacaatgct
     3601 tcgctaagcg taatcatcgt aacgctcttg ttataaatgg gaatggtaat cattattttc
     3661 tcaaatgtgg agcatttctt gccgttacga ctacaatttt ctcaattaat taacgtatcg
     3721 tgccgtttcg ttttccataa atttacaatt ttattaccat aacagcgttt tcccattctt
     3781 aacctggctt ttttcatgaa gattttttcc gtccgacaga ccgttttgcc cgcactgctt
     3841 gtcctttccc ccgttgtttt tgccgctgat gaacagacta tgattgtcag tgccgcaccg
     3901 caggtggttt cagaactgga taccccagca gcagtaagcg tggtggatgg cgaggagatg
     3961 cgcctggcaa caccgcgcat taacttgtcc gaatcactga ccggcgtgcc tggtttgcag
     4021 gtacaaaacc ggcagaacta tgcgcaagat ttacagctgt cgattcgcgg atttggctcc
     4081 cgctccactt acggtattcg cggtattcgc ctgtatgtgg acggtattcc cgccaccatg
     4141 cccgacgggc aagggcaaac atccaacatc gatttaagca gtgtgcaaaa tgtggaagtg
     4201 ctgcgtggcc ccttctctgc cctgtatggc aacgcgtctg gtggggtaat gaatgtcacc
     4261 acccagaccg gacaacagcc accaaccatt gaagccagta gttactacgg cagttttggc
     4321 agctggcgct atgggctgaa agcaacgggc gcaacgggag acggcacaca gcctggcgat
     4381 gtcgattaca ccgtctcaac cacgcgtttt acgacccacg gctatcgtga ccatagtggc
     4441 gcacagaaaa atttagctaa tgccaaactg ggcgtacgca ttgatgaagc cagcaaatta
     4501 agtctgattt tcaatagtgt ggatatcaaa gcagatgacc caggtgggct aaccaaagca
     4561 gaatggaagg ctaatccaca acaagcgcct cgtgcagaac agtacgacac gcgaaaaacc
     4621 atcaagcaaa ctcaggctgg gttgcgctat gagcgtagcc tgagcgcgca agatgatatg
     4681 agtgtgatga tgtatgccgg agagcgagaa acgacccagt accagtcaat acccatggca
     4741 ccacaactta acccgtcaca tgcgggcggc gtgattaccc tgcaacgcca ttaccagggg
     4801 atagacagcc gctggacaca ccgtggtgaa ctgggcgttc cggtcacgtt cactaccggc
     4861 ctgaactacg aaaacatgag tgaaaaccgc aagggctaca ataacttccg cctgaatagc
     4921 ggcatgccgg agtacgggca aaaaggtgag ttgcgtcgcg acgaacgcaa tctgatgtgg
     4981 aacatcgatc cctatttaca gacgcagtgg cagctgagcg aaaaactgtc gctggatgct
     5041 ggcgtgcgct acagctccgt gtggtttgat tccaacgacc attacgttac tccgggtaac
     5101 ggcgatgaca gcggtgatgc cagttatcat aaatggctac ctgccggttc gttaaaatat
     5161 gcaatgaccg atgcctggaa tatctatctg gcagccgggc gaggttttga aacgccgacg
     5221 attaatgagc tgtcttatcg tgctgatggg caaagcggta tgaactttgg tttaaaacca
     5281 tccaccaacg atacaattga gatcggcagt aaaacgcgta ttggtgatgg gctgcttagt
     5341 ctcgcattgt ttcagaccga cactgatgat gaaattgttg tcgatagcag tagcggtggg
     5401 cgtacgactt acaaaaatgc cggaaagacc cgtcgtcaag gcgctgaact ggcatgggat
     5461 caacgtttcg caggagattt tcgcgtaaac gcgtcctgga cctggcttga tgcgacctat
     5521 cgcagcaatg tttgcaatga acaggattgt aacggtaatc ggatgccagg gatcgcccgt
     5581 aatatgggct ttgcgtcgat aggttatgta ccggaagatg gttggtatgc aggcacggaa
     5641 gcgcgttata tgggcgatat tatggcagat gatgaaaata cggcaaaagc gccgtcttat
     5701 actctcgtcg gcttattcac cgggtataaa tacaattacc acaatttaac tgtggattta
     5761 tttggtcgtg tcgataattt attcgataaa gaatacgttg gttctgtcat tgtcaatgag
     5821 tcaaacgggc gatattacga accttcgccc ggacgaaatt atggtgtcgg catgaatatt
     5881 gcgtggagat ttgagtaatt accttgatgc ccggtatttg ccgggcattt actttaacga
     5941 ttgtattgct ggtataaaat agcaatattt tgttgcataa tttcagcaag acaatgtctg
     6001 catccctctt tgtcttttgc ttttaacgca gcgagtaaca gatgatagtt ttctatatgc
     6061 tcccgtaatt ccgctggatt aatcgcttca tagagataat gtaaaccagg ccccatcctg
     6121 acccacagtt gctcaatcat ctcacacagg atgggcatat ttgagcgatg ataaatcgct
     6181 aagcgaaata gcctgtttac attaatgatt tgttccatgt cgcccttttc ttgcgcctgc
     6241 tgtaatttct cgagcaattc ctagagttcc gccaggtctt gcggggtgag gttttcaaca
     6301 gccagagcaa ctgccattaa ttccagttcg tagcggatcc gattgatttc gtctaattga
     6361 cgtttcccca cttccggaac tgtaaatgct tgtgcaggtg cgacagaaag cgcattcacc
     6421 gaaaccagac gtaataatgc ttcacgcaca ggtgtaatac tcatacctaa ttgctccgcc
     6481 agatttttag taatcaggcg tgcgccaggt tttaatgcgc caatactaag ctgatgtttc
     6541 aggccgttct caacctgcag agtcaaactg acatgtttca tttttcccat tccaggcatt
     6601 ttaattcctt cgcgtgtttt ctgtccaacc ctgcctgcac ggcagaagga gttcacctgg
     6661 tgtattaaag tgaataatca taaaccaccc acctttgttg ttattagcag cagaacaatg
     6721 acgtggttag ttaattaaag ctgaaaataa gctttttgcc tggattttta tggcggctta
     6781 cgccgccgtt gaaactttaa tcatcacccg cgacgcggat caccactttg ccgaagtttt
     6841 tacccttcag taggccgata aacgtctgtg gcgcattctc taaaccatca gtaatatctt
     6901 cgtggtagtg gattttatcc tctttcaccc attgccccat ctccttctga aactcatgga
     6961 tgcggtgacc ataatcctga gcgataacaa acccttgcaa gcgaatacgt tttttcagca
     7021 ccgttgccat caacaggggt aaacgatccg gaccgggtgg tagctctgta gcgttatagc
     7081 tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt aagtaatggt agcactgcat
     7141 caaatacctt accgcccacg ttttcataat agatatcaat accttttggg cacgctttca
     7201 ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca aacatcgaag cccagcacct
     7261 cgatagcatg gcggcatttt tccgcgccac cggcaacccc caccaccctg caacctttaa
     7321 gtttgccgat ttgccccacc gttgctccca caggacctgt cgccgcagct accaccaacg
     7381 tttcgccctc tttaggctga ccgatatcca gcagccccat ataagcggta aagcctggca
     7441 tccccagcac acccagcgac cacgatggat tttgcggatg atcgccaagt ttcaccagat
     7501 catcaccact ggatatgtca tagtcctgcc atccactgta gcccagcatc cagtcgccag
     7561 gctgataatc aggatgattc gactccacaa cacggctcac cgtgccgccg accatcacgc
     7621 cgccaatatc aacaggcggt gaataagatg gctcatcgct catacgtcca cgcatatacg
     7681 ggtccaggga caaataaact gtgcgcagta acacctgtcc ttcacccggt gtggcgacat
     7741 catcttcttc aagacggaaa ttctccggaa ccggtgcgcc atgtggacgc gaggccagaa
     7801 cccaacgtcg attacgctgc ttttgttgcc ccataatgct ctcctgtgct tttggcatga
     7861 atccatcaat agtagcaggc tcacgggatt ggttttgttt gcagccgtca catcccctct
     7921 gcaacacgca ataatttcat cgaggaatcc gttgcttcgg gctgttttcg atagtattga
     7981 taacatcatc atcattattt tatcgaggcc agacatgtcc atccgttttg cccgcaaagc
     8041 cgattgtgct gccattgcgg aaatttataa ccacgccgtg ttgtatacgg cggctatctg
     8101 gaatgaccaa acggttgatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc
     8161 aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg acgggatatg cctcgtttgg
     8221 cgactggcgt agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc
     8281 cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc cgattgattg atgaagcgcg
     8341 ggatagcggg aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cctcgctgca
     8401 tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg ccgcaggtgg gcactaaatt
     8461 tgggcgctgg ctggatctga catttatgca gttgcaactc gacgaacgca ctgaaccgga
     8521 cgcgattgga tgaatcagtc gctcaccctt gcttttttga ttgccgccgg aattggtctg
     8581 gtagtccaga acacgctgat ggtgcgcatc acccagacct cctctaccat tctcatcgcc
     8641 atgttgctga actcactggt ggggattgtg ttctttgttt ctattttatg gtttaaacaa
     8701 ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct ggtggacact tatacccggc
     8761 ttgctgggat cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg
     8821 acaactattg cagtactggt cgccagccag ctaattgccg gtctgatgct ggatatcttc
     8881 cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa tttgtggcgc aattttactt
     8941 gtggttggcg cctggttggt agccagacgc tcattttaat cagaggatgg tgccgccttt
     9001 ggttaactgt tcatggcgcg cttccatatc ttctttatgc ttgcgaccgt gatgagcaat
     9061 agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc cgacttaact ccgcgtcatc
     9121 gctcagggcg attaataatt cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc
     9181 cacgcgcgcg atgacttcat ccagatgcga catactttct ccttaaccac ttgtgaaatt
     9241 tatcgattca tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt
     9301 aaaacagtga aaacaacagc gccaccggga aactcatcgg ccatgttgcc cccaccagaa
     9361 aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg
     9421 cgattgctgc cataaccgca taaaaaacaa gcatatgttg atagagagac atgtacgggc
     9481 gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat tttttgacgt gcatcaagtt
     9541 ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg cccttatcca tcttcagaat
     9601 gagtattatt gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg
     9661 gcaaaaatga atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt
     9721 ttgtttactc ttatctctat gagatttggc tggtttattt gatgtagtga ttttgcacgc
     9781 agcacgtact gtcagagaaa tcaactctct gacagtacgc aagatcttct catactctcc
     9841 aagtttacaa cattatcctt aatatttact tctttttgct ggcatctaca ttgaaatgag
     9901 tagaaaacat ccccctactg tcgtctatac aaacagctta ctgattcaaa cggaatcttg
     9961 ttttcacatt tttcta
//
LOCUS       DAJSKJ010000141         9925 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000141 DAJSKJ010000000
VERSION     DAJSKJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.142"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..261
                     /locus_tag="ON345_003008"
     CDS             <1..261
                     /locus_tag="ON345_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="with ArnE is responsible for the transport of
                     4-amino-4-deoxy-L-arabinose-phosphoundecaprenol from the
                     cytoplasm to the periplasm; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phospho-UDP
                     flippase"
                     /protein_id="HCR3073037.1"
                     /translation="FGLDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVL
                     VWIASMVLPGWEGTFSLKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(255..521)
                     /gene="pmrD"
                     /locus_tag="ON345_003009"
     CDS             complement(255..521)
                     /gene="pmrD"
                     /locus_tag="ON345_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR3073038.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(631..1986)
                     /gene="menE"
                     /locus_tag="ON345_003010"
     CDS             complement(631..1986)
                     /gene="menE"
                     /locus_tag="ON345_003010"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR3073039.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(1983..2945)
                     /gene="menC"
                     /locus_tag="ON345_003011"
     CDS             complement(1983..2945)
                     /gene="menC"
                     /locus_tag="ON345_003011"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR3073040.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(2945..3802)
                     /gene="menB"
                     /locus_tag="ON345_003012"
     CDS             complement(2945..3802)
                     /gene="menB"
                     /locus_tag="ON345_003012"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR3073041.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(3817..4575)
                     /gene="menH"
                     /locus_tag="ON345_003013"
     CDS             complement(3817..4575)
                     /gene="menH"
                     /locus_tag="ON345_003013"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR3073042.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLETTSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(4572..6242)
                     /gene="menD"
                     /locus_tag="ON345_003014"
     CDS             complement(4572..6242)
                     /gene="menD"
                     /locus_tag="ON345_003014"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR3073043.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGQLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(6331..7626)
                     /gene="menF"
                     /locus_tag="ON345_003015"
     CDS             complement(6331..7626)
                     /gene="menF"
                     /locus_tag="ON345_003015"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR3073044.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(7705..8010)
                     /gene="elaB"
                     /locus_tag="ON345_003016"
     CDS             complement(7705..8010)
                     /gene="elaB"
                     /locus_tag="ON345_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR3073045.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(8065..8526)
                     /locus_tag="ON345_003017"
     CDS             complement(8065..8526)
                     /locus_tag="ON345_003017"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3073046.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            8591..9508
                     /gene="rbn"
                     /locus_tag="ON345_003018"
     CDS             8591..9508
                     /gene="rbn"
                     /locus_tag="ON345_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR3073047.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            9696..>9925
                     /locus_tag="ON345_003019"
     CDS             9696..>9925
                     /locus_tag="ON345_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR3073048.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPT"
BASE COUNT         2326 a         2834 c         2462 g         2303 t
ORIGIN      
        1 tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct ttccgttttt
       61 tgttggtata aaacgctaca taaacttgcg ctaagcaaag cctatgcctt gctcagtatg
      121 agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg gactttttcg
      181 ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat tttcctgccc
      241 atgacaaaac aacgttactg agttttccct gccactttac actgccgttc ccactcatcg
      301 gggctataac tgcttgccga gaggactttc accgtatgca gcttttcacg attaatacaa
      361 taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa gtcactcttc
      421 acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataagtac atgtctgtta
      481 tcttgtttat tgcagcacga ttttttgacc agccattcca ttgcattatc ctgtttgcta
      541 agagttttca catcaattta cagattaaca gataaactct ttatcaacgg caatggaatg
      601 gcagaaatga gaagtgaaac ggagggttaa ttattgctga cgttgcaccc actcttttag
      661 cgcctgacgt gaaattttaa taccgccgtt tttcagttca ggcggcaacg ttaaccaacg
      721 caccggttgc tgaaaacggg ccagcttatc tttcacccat tcactaagat caacgctctc
      781 gtggtcatac tccatcaccg ccaccggtcg atgaccaaac tccttgtcgg caacggggac
      841 gataaacacc tgcaaaaccg caggatgtgc agcaattacg cgctcgactt cttccggctg
      901 aataccctct ccgccactga agaatagatt gtctaaacgt ccgacaatgg tcagcttgcc
      961 attatgcatc tcaccgcgat cgcgcgtagc gtaccagcct tcgtcattaa ccagtgaaac
     1021 cagttgcccg ttacgccagt aaccttctgc catactggca gcccgcagcc acacttcatt
     1081 attaacgatt ttcacttccc gccccggcag cggcgaacca acgtctgcca ggccgtcggc
     1141 ttctttcgca cacaccgtgg aggcaaactc ggtcagacca tagccgcaaa agcaacgaat
     1201 cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata gccgcgccgc caagtaacac
     1261 cgctttcagg gaaacggaac tgcggttaac cagcaaacgc cagagttgtg ttggcaccag
     1321 tgaagcgtga gtacagcctg ccagcatttg ctccaatggc tgtttatcac gcaccgtcat
     1381 ccgcgcacca gcgtataacc agcgccacat aattccctga ccggagacgt gaaacagcgg
     1441 taaagagagc aaccagtcat cgtgatcgcc aaacggaatc aacgataaca ccccttcggc
     1501 actggcaaga tgggcctgat aagtatggac agcggctttc ggcaacccgg tagaacccga
     1561 ggtcaacgtc attgagcaca gacgcgtcgg ctgccacgta gcggcatgtg cgccttcaac
     1621 ccgctgaatg tgcagcgacg ttaacgccgg aaacgtgttt tccccatccg gcaccagcgc
     1681 aaattgcagc gtcagattgg gcagcaattc ttcaagcaac ggttgcggca gctgagggtt
     1741 cacgggcaac acccgcgccc cgcattgcag taacgccagc caggcgagca gcgtttgcgg
     1801 cgtattccac gcccgcaaca tcacgccgct gccctcaaca acgccctgcg ccgcaaagcc
     1861 tgaggctaat tcatcgacgc gagcacaaag ctcgcgccag ttaagttgct cgtcattaag
     1921 acgtaaggcg atggcttctc cccgcacttg ccgccagtga cgccacggcc agtcagagaa
     1981 gatcataaca accgctccag tgcatcaact tccacgacag gcagcgtgct acccggccag
     2041 cgacgtacct gctgcgcctg catcagatcc agcgtgtcca gccctggaat ggtgtccggc
     2101 gttaaccagg cggcaatccg cgccagttgc gttaagccta agctcgattc aatggaagaa
     2161 ctgatcaccg ccgtcagccc cagcgcgtgc gccgcctgta cctgctcgcg tactttctcc
     2221 agactgcccg tgagcgtggg tttgataact accgcgcgca cgccctcttc agccacaaag
     2281 gcaaaatccg gctcgcgcaa gctttcatcc caggcaatgg caatgccggt ttcacgggca
     2341 aacgctcgcg aatcatcgcg ggttttgcac ggctcttcga gaaacgcgat gcggtggcga
     2401 taatccgggt taacatattt ggcaaactgc tggcctttca gcggtgtcca ggcgcgattt
     2461 gcgtcaagac gcaaatgcag atccggaatt gcctccagca acagattaac caccatgccg
     2521 tcgcgcacgg cttcgtacaa tcccactttg accttcgcca ctttctcgcc tggcatatca
     2581 gcaagtttga ggatcagatc gtcaggatcg ccattacaca gtggtgccgc acggtaattg
     2641 gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac agcttacgcc aaaggcaacg
     2701 gaaggcatct gcggtagctc gcaatcgcct gccagccagt tatttaccca ggcaagcagc
     2761 acgctttgcg cctcttccca ggtttcctgg ctgaagcccg gcagtgggga gatctccccc
     2821 cacccttcgc gctcgccctc acgcaggcaa acgtacagcc cgtcgcgggt ttttaaccgc
     2881 ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta
     2941 cgcattacgg attccgttta aatttgctga aatcaggctg acgtttctgg ttgaaagcgt
     3001 tgcgaccttc ctgaccttct tccgtcatgt agaacagcat ggtggcgttg cccgccagct
     3061 cctgcagccc cgcctgcccg tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg
     3121 ccatcgggct gttttgcagc atttcgcggc accaacggac ggtttctttt tccagatccg
     3181 ccagcggtac cacggtgttg acaaggccca tatccagcgc ctgttttgcg tcgtactgac
     3241 ggcacaggaa ccagatttct cgcgcttttt tctgcccgac gatacgagcc atgtaggaag
     3301 cgccccagcc gccgtcgaag gaaccgactt tcgggccagt ctgaccgaag atggcattat
     3361 ctgctgcgat agtcaggtcg cacatcatgt gcagaacgtg accaccgccg atggagtagc
     3421 cagccaccat cgcgacaacc ggtttcggac aggtacggat ctgacgctgg aagtccagca
     3481 cgttcaagtg atgtacgccg gaatcatctt tatagccgcc gtaatcacca cgcactttct
     3541 ggtcaccacc ggagcagaac gctttatcgc ctgcgccagt cagaatgatc acgccgatat
     3601 tgtcgtcata acgcgcatct gccagtgcct ggatcatctc ttttaccgtc agaggacgga
     3661 aggcattgcg cacctgcgga cgattaatgg tgatttttgc gataccgtcg gtggattttt
     3721 cataacgaat gtcctcgaaa ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca
     3781 ttgcttcatc aggataaatc atattgtgtc ctttagtcag aaacgcaaga tctgcgccag
     3841 acttgcgatt acgccagcgg gattttcccg atgcgcgtta tgtccggcgc gaggaatgac
     3901 atggcagtcg gcagccagtt ccgccgccag ggcgcggaat ttgctgtcac gttcaccaca
     3961 taaataataa aacgcaaatg tgcgggcgct aaggttagca cgtaaatcag gctggacggc
     4021 gagagaagtc gtctccagca tggcggcaag ggttgcgcca ttattgttgc tgcgcagcgc
     4081 caccagctcc cggcgttgat cgtcattgag cgaggcaaaa acaggctgtt gataccagtc
     4141 ggcaaatacc gccgttaacg gttctgtgca aaaacgctgc gcccattggc gatcggaacg
     4201 ctgacgttcc gcacgttgtt cagcattttg cagccccgga tgcccgcctt cgacaataac
     4261 cccacaaagc cccgccagcc cctggcaagc cgccatcatc gccacccgtc cgccaagcga
     4321 gtaccccacc agccagaagt caaggatgtt gtaactaacc aaggttttac gcagtaagtc
     4381 ggtgacatca tcaaatccat cgacgctaat cgccgccgaa ccaccatgac ctgggagatc
     4441 aacatacaac cgtgagtagt cggcaaacgc ctcgcccact tcttgccatt cgtggcaatc
     4501 gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct ggttttccgt gttttgcctg
     4561 cgcgtgcagg atcataaatg gcttacctgc gccagaagtt gctggagcgt ttgcgcacca
     4621 tcggtgtcgt taaccaccat ttcaatcacc gtggtggttg gcgtgcgcca ggcgtcggca
     4681 aatgccgttt caagttcctg ccagttttgc ggacgatgat atttcagctc gaacatcgcg
     4741 gcggcgtgct caaaatggac gttttgcggc atcagataga aacgctcacg ctcgctttgc
     4801 ggcgttggca acagcgagaa aatttgcccg ccgttgttgt tcaccacaat taataccagc
     4861 ggcgcagaaa cctgacgcaa taacgccagc gcgttgagat cgtaaagtgc ggagagatcg
     4921 cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa cgcctgcggc ggtcgaaagc
     4981 agtccgtcga taccgctggc ccctcggttg ctgtacaccg ggtaacctgc cggaagttgc
     5041 gaaagcgcat caatcagacg taccaccagg ctgttaccaa caaacaattg cccctgttca
     5101 ggcagatagt cgctgatgcg atgcgccagt tgcgcttcgc caaacgcatc acggcgggca
     5161 ataaccgcct gcattgcctg ttccgccagg cgcgggattt caacgcacca gggctggcgt
     5221 ttttctgccg gatgcagctc cagccagtcg gcaatattgg caattaagcg acgtccgcga
     5281 tggtgtgccg gatcaagttg cccttcaatg tcatcaacaa tccagtactc ttcgggttca
     5341 cagcttgcct gccattgcag gagccgtttc cccgtcaggc tgcttcccag ttgcaccaca
     5401 atttgcgcct gctgcagctc gctggtcgct ttggcattgc ctaaccaaag atcggcacac
     5461 ggcagcggct gcccggtttg tgacagcaca tcgccaatca gcggccagcc aagagtttgc
     5521 gcccacaggg caactttttt gccctcttcc gcactcatgc gcccggcaac caccacgccg
     5581 cgcttttgtc gccagaagaa ccagtcgcgc tgtttttcac tttccagacg aggcgcttca
     5641 cgcagccacg gtttgtcgtc ctgccaccag tcacccagac gctgttgcca gctaagcccg
     5701 gtatcgtcca tttcgccata cagcggttca gcaaacgggc agttgatatg gactcccccc
     5761 gcatgaagcg taccgagagc gtggtcgatg gtagaaacca gccaacgtgc ggggatatcc
     5821 tgggtcgggc gcggcaatga aatactgtgc gtggggtgag aggcgaacat tcccggctgg
     5881 cgaattgcct gattcgcgcc gcagtcaatt agctccggcg ggcgatcggc ggttaagaga
     5941 atcagttttt ctccggttaa cccggcttca atcagtgccg gatagagatt tgccaccgcc
     6001 gtgccggagg tcacaatcac cgccaccggc tgcttgctga ctttcgccag ccccagcgcc
     6061 agatgcccca gtccacgctc atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc
     6121 gccgctaacg ttaacggtgt agaacgcgag ccaggggcga tacagatgtg tctgacgccg
     6181 tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac
     6241 atgagtttgt ccggtatcaa tattgcggct aagtataagg agctacaaaa atagattatt
     6301 gatatgaatc ggtaatgatg cgactcatta ctattccatt tgtaataaag tacgcagccc
     6361 tgccgctttg ttgtcgattt cctgccactc ttgctcgggg tcggaaccac ggacaatgcc
     6421 cgcgccagca tataatcgca cgacattgcc gctaattttt gctgagcgca gggaaacgca
     6481 gaattcgctt tgttgtaatg agagatagcc cgcagaaccg gcgtaccatt ctcgggtgaa
     6541 cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc ggtaagccag caactgctgc
     6601 cgttggctgc aactgatgta aacagatcac atcatccgct ttgttgagtg aagtccagat
     6661 acagcggcga agatgctgca ctttacgcag acgcagtacc tgcggcggta aaacatccag
     6721 cgtctgggta tcggcctgta atcgttggca gatatcttcc accaccagca tgttctcgcg
     6781 ctggttttta tcatccgcca tcagccactc tcctaactgc tgcgcctgct tatcatcagg
     6841 atgatttgct actgttcccg ccagcgcttc agtacgcagc gctttgtcac gccgccgcca
     6901 taaccgttcc ggtgaagagc caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg
     6961 gtagcaattc agattcagtc gacgactggc agccatcatc gccgccgcgt tgaccggact
     7021 tgcgaaatgc aggtcagttg cccgagcgag caccactttg tcgagctcac cttcggcgat
     7081 ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc
     7141 tcgcgtggtg gttaaatgta acccaggcaa gggcttgata ctcaccagtg tggcgataaa
     7201 ttcttttgcc tgaatcgcat cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag
     7261 cgtggcttta ccgccacagc gtcgccattc caggcggggt aaaagtaaat tgccctgcga
     7321 cgggtcaaaa gcattcagcc cccaaatgcg taagtcggcg tgttccgggt gctggcgaag
     7381 aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc gcgcccagga cgacagcttc
     7441 ttcatcacca ttacgttgtt gccagtagaa ttgcgggtat gtttgctgac tggccagcca
     7501 gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata tcgataaccc gaatgccggg
     7561 tgtcgccgga atctcttgcg acaaatggcg cagtagattt tccagcgccg tagtaagtga
     7621 ttgcacgcgg acctctccct gttaaaagcc tcacattata cggggtacta caaaaaaaat
     7681 gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc aacagtccta gtaccagccc
     7741 aacggccgcg cccacaccaa ttccttgcca gggtttttcg tggacgtagt catcagcacg
     7801 ataaactgcc tgcttcgccc gatagtaata actgtctgaa gcctggctaa cccgtttttt
     7861 cacatcatcc agcgcttttt ctgcacgcgc tttcagctca acatatttct gatcggcggg
     7921 atcgccagag gagcggagca cctcttccag tgtttcacta agcagcgtca ggtcgtcatc
     7981 gatacgtgta tcaccaaact gattagacat tctcgttctc catttgcgta aaacctgtca
     8041 ctaactatag acaatggttg ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg
     8101 tgggatacca tcttcttcat agacctccgt cacagggata aaaccgaaac tctggtagaa
     8161 gttttgcaga tgcgcctgcg ccccgaggta aacaggctta tcaggccagt gatgcgtaca
     8221 tgtttcgagt gttttactca tcagctgctg ccccactttt tcgccacgca acgcttcact
     8281 gacaatcacc cgacctataa cgaccggctc aagatcatca tcacttttca gaatcctcgc
     8341 atatgccacc agttcatcat ttttccaacc gagaatatgg cgattatccc ccgtcaaatc
     8401 atcaccatcg atatcctgat aagggcaatt ctgctcgaca acaaacaccg cgcaacgcag
     8461 ttgtaataag gcataaagct gggaaaccga taattcagag tgatgcagat cttgccattc
     8521 aatcataatg ctctcctttt agatgtatgc cttatactag ccggaatatt ttttgaaacg
     8581 tgatgaactc atggaattaa tttttttagg tacttcggcc ggtgtgccaa cccgcacgcg
     8641 caatgtcacg gcaatattgc ttaacctgca acatccgacc cagagcggtc tttggttgtt
     8701 tgactgcggt gaaggcaccc agcatcagct actgcatacc gcctttaacc ctggaaaact
     8761 ggacaagatt tttatcagtc accttcatgg cgatcatctt tttggtttac ccggcttgct
     8821 gtgcagtcgt tctatgtcag gcattatcca acccttaacg atttatggtc cgcaaggtat
     8881 ccgtgaattt gtggaaaccg cgctgcggat tagcggctca tggaccgatt atccgctgga
     8941 aattgtcgaa attggcgctg gcgaaattct cgatgatggc ctgcgcaaag taaccgctta
     9001 tccgctggaa cacccactgg aatgttatgg ctatcgtatt gaagaacatg ataaaccggg
     9061 tgcattaaat gcccaggcat taaaagctgc tggcgtgccg cctggcccac tgtttcagga
     9121 attaaaagcg ggcaaaacaa tcacgctgga agatggaagg cagattaacg gcgcagatta
     9181 cttagctgct ccagtgccag gtaaagcgct cgctattttc ggcgataccg gcccctgcga
     9241 tgccgcactt gacctggcta aaggtgtcga tgtcatggtg cacgaagcga cgctggatat
     9301 aaccatggaa gccaaagcca atagtcgcgg ccatagctct acacgccagg ctgcgacact
     9361 agcccgtgag gctggagtcg gcaagctaat cattacccac gtcagctcgc gctatgatga
     9421 caaaggttgt caacacctgt tacgtgaatg caggtcaatt ttcccggcga ctgaactggc
     9481 gaatgatttc accgtgttta acgtttaact ctgcttatta ttaaaaacag ggtgaaactt
     9541 gccctgttat agcaacccgc gctacatata tccacccaca caggcatatt gagcgatttt
     9601 tgatatttat tcatctcctt acgcactata ttcagagcaa taagttgcct cttttcatct
     9661 tgattcagca caacctttgc aactaaaagg agcccatgat ggttacagtt gtcagcaatt
     9721 attgtcaatt atctcaaacc caactcagtc agacatttgc agaaaaattt actgtgaccg
     9781 aggaattact gcagtcttta aaaaaaacag cgttatccgg agatgaagaa agtattgagt
     9841 tactgcataa tattgcgtta ggttatgatg aatttggaaa aaaagctgaa gatattcttt
     9901 accatattgt tagaaaccca acaaa
//
LOCUS       DAJSKJ010000142         9892 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000142 DAJSKJ010000000
VERSION     DAJSKJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.143"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(218..293)
                     /locus_tag="ON345_003020"
     tRNA            complement(218..293)
                     /locus_tag="ON345_003020"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Lys,seq:ttt)
     gene            complement(296..371)
                     /locus_tag="ON345_003021"
     tRNA            complement(296..371)
                     /locus_tag="ON345_003021"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(336..338),aa:Val,seq:tac)
     gene            complement(407..482)
                     /locus_tag="ON345_003022"
     tRNA            complement(407..482)
                     /locus_tag="ON345_003022"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(447..449),aa:Lys,seq:ttt)
     gene            complement(647..1438)
                     /gene="cpoB"
                     /locus_tag="ON345_003023"
     CDS             complement(647..1438)
                     /gene="cpoB"
                     /locus_tag="ON345_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR3073049.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1448..1969)
                     /gene="pal"
                     /locus_tag="ON345_003024"
     CDS             complement(1448..1969)
                     /gene="pal"
                     /locus_tag="ON345_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR3073050.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2004..3296)
                     /gene="tolB"
                     /locus_tag="ON345_003025"
     CDS             complement(2004..3296)
                     /gene="tolB"
                     /locus_tag="ON345_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR3073051.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3429..4694)
                     /gene="tolA"
                     /locus_tag="ON345_003026"
     CDS             complement(3429..4694)
                     /gene="tolA"
                     /locus_tag="ON345_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR3073052.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4759..5187)
                     /gene="tolR"
                     /locus_tag="ON345_003027"
     CDS             complement(4759..5187)
                     /gene="tolR"
                     /locus_tag="ON345_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR3073053.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5191..5883)
                     /gene="tolQ"
                     /locus_tag="ON345_003028"
     CDS             complement(5191..5883)
                     /gene="tolQ"
                     /locus_tag="ON345_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR3073054.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5880..6284)
                     /gene="ybgC"
                     /locus_tag="ON345_003029"
     CDS             complement(5880..6284)
                     /gene="ybgC"
                     /locus_tag="ON345_003029"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR3073055.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6434..6727)
                     /gene="ybgE"
                     /locus_tag="ON345_003030"
     CDS             complement(6434..6727)
                     /gene="ybgE"
                     /locus_tag="ON345_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR3073056.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6727..6840)
                     /gene="cydX"
                     /locus_tag="ON345_003031"
     CDS             complement(6727..6840)
                     /gene="cydX"
                     /locus_tag="ON345_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR3073057.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6855..7994)
                     /gene="cydB"
                     /locus_tag="ON345_003032"
     CDS             complement(6855..7994)
                     /gene="cydB"
                     /locus_tag="ON345_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR3073058.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8010..9578)
                     /gene="cydA"
                     /locus_tag="ON345_003033"
     CDS             complement(8010..9578)
                     /gene="cydA"
                     /locus_tag="ON345_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR3073059.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2429 a         2699 c         2360 g         2404 t
ORIGIN      
        1 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
       61 aatcacccga tactgcgctg gatactacaa actacttcca ccgaacccac cgtaattagt
      121 ggtgggtcgt gcaggatgac tcgcttcgct cgccctacgg gccgttgctg acgcaacgtt
      181 atcctacacg cttaacatct gtcttcgatg ctaaaactgg tgggtcgtgc aggattcgaa
      241 cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga cccggtggtg
      301 ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga
      361 gctaatcacc caatactacg ctggatacta caactaacta tgttagtggt gggtcgtgca
      421 ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac
      481 ccactttttc gttgctttcg gtttgtttga tatcccgtgg caacggcggc atatattact
      541 gatttcagac ttgagcgcaa caaaaatttc gatgcagatc actcaactgc ttatgattcg
      601 cacgacacga ccagaaataa tgcgacttct ggtcgtgtgt tatgcattac atcgcgttca
      661 gacgtttttg tgcctgttta gcgccatcag taccagggta tttactgata acctgctggt
      721 atacggcttt cgcttttgcg gtgtcacctt tgtcctgcat gatgacgccg actttaaaca
      781 tcgcatcagc agcctttggt gacttcggat agtttttcac taccgaagca aaatagtacg
      841 ccgcatcatc ttttttaccc ttgttgtagt ttaactgacc cagccaataa ttggcgtttg
      901 gcaggtaagt tgaatcaggg taatttttga tgaaattctg aaatgccacc attgcgtcat
      961 cctggcggga tttatcctgc accagcgcaa tagctgcatt gtaatccgtg tttgcatcgc
     1021 cgcttttcac cggcgcgcca gcattcgcag taccagcatc agctgtcggc gtcgttgatg
     1081 ccgccgcacc gctttgatcg ctgctggttg attgcgccgc tgcaccaccg ctgctgaggc
     1141 tgtcgatctg caacaggatc tgcttttgcc gctccacgac ctgattcagt tgatactgat
     1201 tttcctgaat ctgaccacgc agggaatcaa tatcggattg attatcagaa agttgttgct
     1261 ggagttgggt taaaagctgg ctgtgagcat tagaaatacg ctcaagttga gtgacgcggt
     1321 cttcgaccga gcctgagccg acactactga ttggtgcctg agcaaaagcg gcccaggggg
     1381 ccgctatacc aaccagtaac gacagactca atagttgatg tctgaagtta ctgctcatgc
     1441 aattctctta gtaaaccagt accgcacgac ggtttttgga gtatgccgct tcgtcatgac
     1501 ccagtactgc aggtttttct ttaccgtaag aaacgatgga gatctggtct gcagaaacgc
     1561 ctttaccctg caggtacatc ttaacggcgt tcgcacgacg ttcacccagg gagatgttgt
     1621 attccggagt accacgttcg tccgcgtgac cttctacggt gactttgtaa gacgggttgc
     1681 tacgcaggaa gtttgcatgt gcatccagca tttgagcgaa gtcagaacgg atatcgtact
     1741 tgtccagatc gaagtaaacg atgttgttct gctgcagctg ttgcatttgc agacgagcct
     1801 gctcttcgga agacatgttg ccgttgccgc cgttcgcatc cataccagtg ccggcaccca
     1861 gcatgccttc gctgccgtca ttgctggcgt tcttgttgga agaacatgcc gcaattgcca
     1921 taacaggcag agcaatcatc agccctttca gcactttgtt cagttgcatt tcaatgattc
     1981 ctttactatt caattaatta ttatcacaga tacggcgacc aggcagggaa tttgacctgt
     2041 ccatcagttg ccggaagacg cgctttgaaa cgcccatctg tagaaaccaa attcagcacg
     2101 gatcccatcc cctgagaaga gctgtagatt accatagtgc cgttaggtgc cagacttggc
     2161 gtttcatcca ggaacgtgga cgacagaact tgtacgcctc ccgttgccag atcttgtttg
     2221 gcaatgtgct gctgcccacc attggagctg accattacca taaatttacc gtcgctgctg
     2281 acatccgcat cctggttctg cgaaccttcc caggtaatac gttgtggcgc accgccgttg
     2341 atattcactt tataaacctg cggacgaccg gcctggtcag aagtaaatgc caggttctgg
     2401 ctatccggga accaggtcgg ttcggtattg ttactgcgac catcagtcac ctggcggatc
     2461 tgaccagaag ccaaatccat tacgtacagg ttcagactac cggttttcga caaggcgaat
     2521 gccagtttgc tgccgtctgg cgagaatgca ggtgcaccgt tgtgacgcgg gaatgaagcc
     2581 acctgacgta cagcgccatt tgccagcgtc tgaataacca gcgcggaacg accgctttcg
     2641 aaggtcacat aagccagttt agaaccgtct ggtgaccacg ccggagacat cagcggctgc
     2701 ggtgaacggt gaacgacaaa ctggttgtaa ccgtcatagt cagatacgcg cagttcatac
     2761 gggaactgac cgccgttggt ctgaacaacg taggcaatac gggtacggaa cgcaccttta
     2821 atgccggtca gtttttcaaa cacttcatca ctggcggtat gaccagcata acgcagccac
     2881 tgcttgttca ctttgtacga gttctgagca agtacagtac ccggtgcgcc gccagtgtca
     2941 acaagttgat aagcaacatt gtaagaacca tccggattcg gagtgacctg accgacaact
     3001 acagcgtcaa tgcccagtgc ggaccatgca gctggttgta cttcctgcgc actacccggc
     3061 tgctgtggca gacgagcgcg atctaacgga ttaaatttac cgctgttacg caagtcagca
     3121 gcaacgatgc cgccaatatc ttcaggtgcc gcaccaggcc ccgcccactg gaaaggaaca
     3181 acaccaatag gacgaccgga atctacaccg ctgtcgatca caatgcggac ttcagcatgc
     3241 agaactgatg cccacagtat gagaaaacca aatgctactc gtaatgcctg cttcatcata
     3301 tctcccttat ctggaccgat ggcccacgat aatttagcag aatgttaaca aactaaaata
     3361 cacaaaacta ccagaacccc gtggcaaccg gtgcctgatg ttgaccgtcc gaacagtcaa
     3421 catcgcgatt acggtttgaa gtccaatggc gcgtttttga acacttcata tactgcctgg
     3481 cttggtggtt tcgggatctt cgcaagttta gctgctgcca acgcagcctg acaaagtgcg
     3541 ggatcgccac cttcaggctt gatatccagt aacataccat cgggtgccag ttttatgcgc
     3601 agcgtacagg ttttgcctgc ataggacgat gcgtcataga acttactttc gatagcagat
     3661 ttaatctgcc cggcatagtt attgatatcg gcccctgatg cgccattgtt tttagtatta
     3721 ccactcccgg caggcgaagc attgttccct ttcgcccctc cccccgtttt cggtgcattc
     3781 ttaccagagc ttagctcacc gaaaatatca tctgcctctg cggccgcttt tgctgcagca
     3841 gctttttcag ctgcagcttt tgccgctgcc gcttttttgt ctgcggcggc tttttctgcc
     3901 gctgctttct tatcagctgc tgccttttca gcagccgctt tttctgctgc ttttttgtcg
     3961 gctgcagctt tctctgctgc cgctttctta tcagctgcag ccttttcagc agccgctttc
     4021 ttctcagctt ctgctttggc tttttcagca gcttcagttg ccgctttctt tcttgcttca
     4081 gctgcagctg cttctgccgc ttccgctttc ttcttcagtg ccgcagcggc tgcctcggct
     4141 tttttctgcg cttcggctgc ggctttggcg gcttctgctt ctgctttttt ctttgcgtct
     4201 gcagccgctt tcttcgctgc ttcttccgca gctttagcat ctgcttcggc cttcgcttta
     4261 gcatctgccg ccgctttcgc tgccgcctct tcagcttgct tctgctttaa ctcggcctgt
     4321 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
     4381 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
     4441 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
     4501 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
     4561 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
     4621 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
     4681 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
     4741 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
     4801 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
     4861 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
     4921 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
     4981 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
     5041 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
     5101 caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact tgagatcgcg
     5161 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
     5221 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
     5281 tttgtttacg cgctggttga ggcggttata agccattacc gccggaattg cggcgaacag
     5341 accaattgca gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
     5401 ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag
     5461 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
     5521 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
     5581 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
     5641 ttgttccgaa ccagtcagat tatcccgttt cccctggctc tcttgataga ggcgagagag
     5701 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcgcgcgccg ctgcgttaag
     5761 aatacgggtc cgctggataa taatggccca agatgcgatt gaaaaaccaa tcaaaatcaa
     5821 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
     5881 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
     5941 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
     6001 cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
     6061 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
     6121 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
     6181 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
     6241 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
     6301 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
     6361 tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa
     6421 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
     6481 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
     6541 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
     6601 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
     6661 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaagtc gcgataatct
     6721 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
     6781 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
     6841 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
     6901 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
     6961 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
     7021 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
     7081 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
     7141 atacgtgcag tcaggatggt cagcagcggc agaaccacac ccagtgccgg aatagcccac
     7201 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
     7261 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
     7321 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
     7381 tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg cagataggtt
     7441 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
     7501 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
     7561 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
     7621 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
     7681 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
     7741 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
     7801 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
     7861 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
     7921 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
     7981 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
     8041 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac
     8101 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
     8161 aagatgagat cgcctgcggt cagtgacgag ttcgccacag ctgtcggcag cacttcaccg
     8221 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
     8281 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
     8341 cggatgacac tccagaaaga gagcgcgatg attgccagaa gcaggaagcc acacgccacc
     8401 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
     8461 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
     8521 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
     8581 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
     8641 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
     8701 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
     8761 ccgaacagag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
     8821 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
     8881 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
     8941 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
     9001 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
     9061 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
     9121 ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga accgagcgcc
     9181 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
     9241 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
     9301 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
     9361 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
     9421 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
     9481 agcaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
     9541 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
     9601 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
     9661 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
     9721 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
     9781 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatatctaa
     9841 attaccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat gc
//
LOCUS       DAJSKJ010000143         9845 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000143 DAJSKJ010000000
VERSION     DAJSKJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.144"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..641
                     /locus_tag="ON345_003034"
     CDS             <1..641
                     /locus_tag="ON345_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3073060.1"
                     /translation="FFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVGLMGP
                     LAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRK
                     GVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMT
                     VQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            693..1922
                     /gene="sorE"
                     /locus_tag="ON345_003035"
     CDS             693..1922
                     /gene="sorE"
                     /locus_tag="ON345_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR3073061.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHKGIWS
                     GEAEQYLLTHAEAISHD"
     gene            2305..2994
                     /gene="pepE"
                     /locus_tag="ON345_003036"
     CDS             2305..2994
                     /gene="pepE"
                     /locus_tag="ON345_003036"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR3073062.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(3085..4716)
                     /locus_tag="ON345_003037"
     CDS             complement(3085..4716)
                     /locus_tag="ON345_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR3073063.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDVMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESSRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDKGRDEY"
     gene            complement(4936..8619)
                     /gene="metH"
                     /locus_tag="ON345_003038"
     CDS             complement(4936..8619)
                     /gene="metH"
                     /locus_tag="ON345_003038"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR3073064.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            8819..9643
                     /gene="iclR"
                     /locus_tag="ON345_003039"
     CDS             8819..9643
                     /gene="iclR"
                     /locus_tag="ON345_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR3073065.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2247 a         2706 c         2586 g         2306 t
ORIGIN      
        1 tgttcttcaa taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg
       61 aagaggcgcg ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg
      121 gtctgatggg accattggca ggggttggcg atccactggt ttggggaacg ctgcgcccga
      181 ttaccgctgc gctcggcgca tctctggcac tttctggcaa catcctcggc ccgcttttgt
      241 ttttctttat tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct
      301 ttcgcaaagg ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg
      361 aaggcgcgtc gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacatcaa
      421 tcaacgtacc gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcaccatga
      481 ccgtgcagaa cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctgc
      541 ttatggttcg tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg
      601 gcttagggat tatcggtaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac
      661 tgccggggcc atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg
      721 gtaaacgtga tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc
      781 tcgcgacggt ggtcactgac agcctgtgcc tctcttcctg gaaagaggcc aatctgggtg
      841 aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc catcatcatc ggccacgagt
      901 tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag ccgggtcagc
      961 gttatgtgat tcaggccaac ctgcaactcc ccgaccgccc ggactgcccc ggctactcct
     1021 tcccgtgggt gggcggcgaa gccacgcatg tggttattcc caacgaggtc atggaacaag
     1081 attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcactggtt gaaccgcttt
     1141 cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt tataaccaca
     1201 cgatggggat tcgcccgcaa gggcgcatgc tgatcctcgg cggcaccgga ccaatgggac
     1261 tgttggcgat tgattatgcg ctacatggac ccgttaaccc gtcgctgctc gtcattaccg
     1321 ataccgacaa cgataaattg agttatgcgc gcaagcacta tccatcagaa ccgcaaacac
     1381 tgattcatta tctcaatgcc gccgatgcag catttgatac gctaatggcg ctgagtggcg
     1441 gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg actctcgcct
     1501 cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag gataaacatt
     1561 tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac gtgggcacgt
     1621 caggcggcaa taccgacgac atgcgcgcgg cggtcaaact gattgaagag aaaaaagtgc
     1681 aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa accacgcttg
     1741 aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga
     1801 cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt cataagggga
     1861 tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt tcccatgatt
     1921 aatcgcgcta cttaactctg catgtcgctg gctgatcgcc gcggcaactt cgcgacctga
     1981 tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa acatcagcgc
     2041 cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat gcggaataaa
     2101 gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct ggtaggcctg
     2161 gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata
     2221 cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca tgcctcccgt
     2281 gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc gacgctgccg
     2341 ggtaaagcct ggctggaaca tgcgctgccg ctgattgctg aacagttgca gggtcgccgt
     2401 tcagcggtat ttattccttt cgctggcgta acgcagacct gggatgatta cacagcgaaa
     2461 acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat
     2521 cccgttgccg ctattgaaaa tgctgagatc gtgattgtcg gcggcggtaa tactttccag
     2581 ttgctgaaac agtgccgcga acgcgggctg ctggcaccaa ttactgacgt ggtgaaacgt
     2641 ggcgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac tattcgtacc
     2701 accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg
     2761 caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag
     2821 cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa
     2881 ggtaactgga tcacagtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat
     2941 gtgtttaagg ctggtgaaga agcggttccg ctggaagctg gtcaccgttt ttaagtgaat
     3001 tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt
     3061 atcaggccta caggattcac aatattaata ctcgtcccgt cctttatctt catccggctg
     3121 ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc aggcgctgca tatctcccag
     3181 taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc
     3241 gacgtgggcg tgggaatagc ggcgattaag aatgcgaaaa cgatgtttgc tgcgacgcaa
     3301 acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagtt ttaaattact
     3361 gagcaattgc tcatagagcg catccagttc cttcaagcca tcaagcgaaa atgcccgccg
     3421 tgctgccagc gatttatcgg caatttcgct gcccatgcgc tcgacgatat cggaagcctg
     3481 ttcaaggttg agcgacattt caataatctc cgcccagcga ctcgactctt cttctgccag
     3541 ttcctctttt ggcatccgcg ccagatacag cttaatggcg gtatagagaa cgttgatatc
     3601 atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag
     3661 cccttccatc atctgttcca tgacgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc
     3721 cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt
     3781 atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa
     3841 tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc
     3901 cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat
     3961 aatcaggcta cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc
     4021 attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac
     4081 cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg cagtcagcaa
     4141 tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag
     4201 cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg
     4261 cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac
     4321 gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa
     4381 aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt
     4441 cccgacatct gcacccagca caatgaccag agccggtgcg agggctacca gatcctgagc
     4501 gacaaacgag gtcaccagca tggtggtggc attactgctc tgcaccagtg cggtaacgcc
     4561 gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa
     4621 acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag
     4681 ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta
     4741 tatgtgcctg aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttat
     4801 tgtgactttt gacattcgtt tttccggtta agattattcc agcctgattc cagtataagt
     4861 gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag
     4921 tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac
     4981 ttcggtaacg ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg
     5041 cgctaccgcg taatacttgc tgtccgggtg gctgaagtac caacccgaaa ccgatgcacc
     5101 aggccacatg gcgaaagatt ctgtgagttt catgccagtg tgtttttcca cttccagcag
     5161 ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc ggatagcccg gtgccggacg
     5221 gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata
     5281 gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt ccgccagacg
     5341 gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc
     5401 agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt
     5461 accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc
     5521 ggtctgttga cgcagatggt ggctgacgtt gatcacatga gtgcgcgttt cgtcacggta
     5581 gatttcaatg tcatcgccca cacggtttgc cgggaacagg cccaccacgc cacgcggatt
     5641 cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg
     5701 cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca
     5761 ggtcataaag aacggtgtcc agtcgatgta attacgcagc gtttcgatgc tggcttcgac
     5821 ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa
     5881 atcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg
     5941 ctgaatacgt acggtttcgt actccttgcg ggtacgagcg acaaaatcat cacgctgggt
     6001 atcggaaagc agcgccgcca ccacaccaac ggtacgcgag gcattctgca catacaccgt
     6061 cgggccgctg tagttctgct cgattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc
     6121 gccaatcaat agcggaatag tgaagccctg acgctccatc tctttcgcca cgttaaccat
     6181 ctcgtccagc gacggcgtga taagccccga aaggccaatc aaatcagcat tcacttcttt
     6241 ggcggtacgg agaatttttt ccgcaggcac catcacaccg agatcgacaa tttcgtagtt
     6301 gttacattgc agcaccacgc caacgatatt tttaccgatg tcgtggacat cgcctttcac
     6361 ggtggcgatc accatcttgc cgttggtttt accctgctct ttgctggctt caataaacgg
     6421 ttcgagatac gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa
     6481 cattttccct tcgccaaaca gatcgccgac cacgttcata ccgtccatca gcgggccttc
     6541 aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa
     6601 ctcggtaatg cctttgacca gcgagtattc cagacgttta ttcacgtccc acgagcgcca
     6661 ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag
     6721 ctccagtaaa cgctcggtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc
     6781 gcgcagttca gcgggcaggt cgtcgtaaat cgccagctgc ccggcattga cgatccccat
     6841 atccatgcca ttgcgaatag cgtagtagag gaacactgcg tgaatggctt cgcgcaccgg
     6901 atcgttgcca cggaacgaga aagaaacgtt agatacgccg ccggaaatta gcgcgtgcgg
     6961 cagttcgcgt ttgatgtctt cacacgcgcc gataaagtcc tgcgcgtagt tgttgtgctc
     7021 ttcaatgccg gtggcaaccg cgaagatgtt cgggtcgaag atgatatctt ctggcggaaa
     7081 gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg
     7141 ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc actgccgcac cgtaacgacg
     7201 caagagcttc gcgtgatgga taaaggcgtc gacgccctct ttcatcgaga tagagttaac
     7261 aatgcctttg ccctggatac acttcagacc tttttcaatg acatcccatt ttgaggagtc
     7321 gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac
     7381 catcgccgct tcggcgtcga gcatcccttc atccatgttg atatcgataa tctgcgcgcc
     7441 gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag
     7501 gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct
     7561 atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg
     7621 cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt
     7681 ggtgccacag cagccgccga cgatattgag aaaacccgct tgcgcccatt cacgtatctg
     7741 ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt
     7801 cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag
     7861 ttcatcgggc cccagcgcac agttcaggcc aaaggtcaga gcttcggcgt ggcgcaatga
     7921 gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt
     7981 gccggagatc ataatcggca gctcaacgcc cagcgcttca aactccgttt tcaccgcaaa
     8041 aaccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcgcc
     8101 accttccacc agcgctttgg tggactctcg ataagccgcc accagcccgt caaaagtgat
     8161 attacgaaat gccggatcat ttacgtccgg agaaatagac gccgtgcggt tggtcgggcc
     8221 gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact catcagcaca
     8281 agctcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta
     8341 atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc
     8401 ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttgctgagca ccagcaggtc
     8461 gttgttgcct ttgagatcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc
     8521 gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc
     8581 atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa
     8641 cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg
     8701 accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt
     8761 gcattagcta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg aggctgtcat
     8821 ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac
     8881 tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa
     8941 tggcagtgtg gcactcacag agctggcgca acaagccggg ttacccaatt ccacgaccca
     9001 ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggtcggcg aactgggaca
     9061 ttgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt
     9121 gttagcgatt gttcacccta tcctgcgcaa tttaatggaa gagtctggcg aaacggtcaa
     9181 tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgaccagg tacagtgtac
     9241 gcatctgatg cgaatgtccg cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc
     9301 aggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa
     9361 agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct
     9421 cgcccaaacg cgcaaacggg gttattcatt tgacgatgag gaacatgcgc tggggctacg
     9481 ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc
     9541 cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcaa tggtgattaa
     9601 agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag
     9661 aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgta
     9721 ttgacgccag aataacttaa tgtcatacac ttcacacact tttattaact cggcattatt
     9781 tttaaacatc aaaccacttt aattattaac aaaatcgcaa gatctcattc cgccgaaact
     9841 tttac
//
LOCUS       DAJSKJ010000144         9792 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000144 DAJSKJ010000000
VERSION     DAJSKJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.145"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            148..609
                     /gene="osmC"
                     /locus_tag="ON345_003040"
     CDS             148..609
                     /gene="osmC"
                     /locus_tag="ON345_003040"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR3073066.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKSKVLPDDASGA"
     gene            complement(634..1560)
                     /locus_tag="ON345_003041"
     CDS             complement(634..1560)
                     /locus_tag="ON345_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3073067.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(1553..2539)
                     /locus_tag="ON345_003042"
     CDS             complement(1553..2539)
                     /locus_tag="ON345_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3073068.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCIHRGQISLLGEDVLNAQEKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2536..3432)
                     /locus_tag="ON345_003043"
     CDS             complement(2536..3432)
                     /locus_tag="ON345_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR3073069.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3429..4451)
                     /locus_tag="ON345_003044"
     CDS             complement(3429..4451)
                     /locus_tag="ON345_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3073070.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFYALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4453..6003)
                     /locus_tag="ON345_003045"
     CDS             complement(4453..6003)
                     /locus_tag="ON345_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3073071.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTNKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVINDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(6017..6598)
                     /gene="ddpX"
                     /locus_tag="ON345_003046"
     CDS             complement(6017..6598)
                     /gene="ddpX"
                     /locus_tag="ON345_003046"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR3073072.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(6856..9255)
                     /gene="dosP"
                     /locus_tag="ON345_003047"
                     /gene_synonym="pdeO"
     CDS             complement(6856..9255)
                     /gene="dosP"
                     /locus_tag="ON345_003047"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR3073073.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEAQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPMVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMDEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(9280..>9792)
                     /gene="dosC"
                     /locus_tag="ON345_003048"
     CDS             complement(9280..>9792)
                     /gene="dosC"
                     /locus_tag="ON345_003048"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR3073074.1"
                     /translation="SRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
BASE COUNT         2392 a         2550 c         2486 g         2364 t
ORIGIN      
        1 atatattccc ggttatctat tgtggaaatt taatttaagt gcagaagtaa tatttctgcc
       61 ggatttttat tcggaatatc ctgcttatcc tcgtgctgtt tctcacgtag tctataattt
      121 cctttttaag cccacaggag agcaacaatg acaatccata agaaaggtca ggcacactgg
      181 gaaggcgata tcaaacgcgg gaagggaaca gtatccaccg agagtggtgt gctgaaccaa
      241 cagccgtatg gatttaacac gcgttttgaa ggcgaaaaag gaactaaccc tgaagaactg
      301 attggcgcag cgcatgccgc atgtttctca atggcgcttt cattaatgct gggggaagcg
      361 ggatttacgc caacatcgat tgataccacc gccgatgtgt cgctggataa agtggatgcc
      421 ggttttgcga ttacgaaaat cgcactgaag agtgaagttg cggtgccggg tattgatgcc
      481 tctacctttg acggcatcat ccagaaagca aaagcaggat gcccggtttc tcaggtactg
      541 aaagcggaaa ttacgcttga ttaccagttg aaatctaaag tattgccgga tgacgcgtca
      601 ggcgcgtgaa tgcctgatgc gttgttagca tctctacagc gcccgccagc accggatctc
      661 acgtccgtcc tcccttattg ttaatgattg ccggacttca catccgtggg ttgccagagg
      721 gcaacgttcg tagaaaaaac agccttgcgg caacgtgcgg tttcccggca gatccgtttt
      781 acgtaatgcc cattcttcct ccagcggttt atcaatggcg gggagggaat ccagcaataa
      841 tcgggtgtat ggatgtgccg gagcagtcaa cacctgttgc gcttccccta gttccacaat
      901 ctgcccgaga tacatcaccg ccacccgatc gctcatatga cgtatcaccg agacattgtg
      961 tgaaatcagc acataagtca gcccgtgatt ttcctgtagc gttaccagta aattgaggat
     1021 ctgcgcctgc acggagatat ccagcgccga ggttggctca tcaagcacaa tcacgtcagg
     1081 ctgcgaagag agtgctctgg caatggcgat gcgttgccgc tgcccgccgg agaacgcatg
     1141 aggcaggcgg tcgagatact ccggacgaat acccacctgc acagccagct cctctgccaa
     1201 cgctcgccgc tgttgttcac tactacgctt agctatccag agtggttctg tgatgatgcg
     1261 ccacaccggt aagcgcggat taagcgaaga gagcgggtcc tgaaacacca tctgcataat
     1321 gcgttgtgag cctgaacgga tgtactgccc gtggctcggt tgcagcatgc ccattaaaag
     1381 ctgtgcgagg gtgcttttgc cgcagcctga ctcgccgacg atccctaagg tttcaccacg
     1441 gcggatctgt aaatcaatac cattaatggc atgaacatgt tccgtagttt taccaagcca
     1501 gtttttacgg gccgggaaat tgatatggac gtcgcgtaac gttaataacg tgtcagacac
     1561 ttatgacctc ctgctgcgga taccaacagg cgcagcgctg gttgttgtca ccacacgctg
     1621 tcagcgccgg gacgttttca cactgtgcac ctgccgcata gcaacgatcg cgaaaagcgc
     1681 agccgtcagg caaatgggtg aggtttggta ccgtccctgg aatggcgggt aatggctggc
     1741 gtggtactcc atgttctggt gcgcattgca gcaaaccaat ggtatacgga tgccggggat
     1801 gatggataat gtctgccgtc acgccgcttt caattacgct tccggcatac atcacgtaaa
     1861 cgctatcgca cagttgcgac accacgacca tatcatggct gatgaacagt accgcagttc
     1921 cactggcgcg ggctttatgt ttaagcaaac gcagtacctg caactgtagc gtgacgtcca
     1981 gcgccgtagt cggttcgtcg gcaataatca attgcggctc gcaggagaat gccagcgcaa
     2041 tcattacccg ctggcgcata ccacctgaaa gctcaaacgg atagcgcgac ataacttcca
     2101 cggcatccgg gatttgcatc tcttccagca gggcaatcgc tttagctctg gcttcccgac
     2161 gacttattgg ttgatgatgg cggatcacgt ccatcatctg aagacctatt cgacgtgtcg
     2221 gattgagggc ggtcatcggt tcctgaaaga tcatcgccac tcgtgcgccg cgccactgac
     2281 gaagctgttt ttcctgcgcg ttaagaacat cttctcccag cagggaaatc tgcccacgat
     2341 gtatgcaata actgcccgtc ggtagcagac gcataatcag cattgcggtg actgatttac
     2401 ctgagccgga ttctcccacc agaccgacaa tttcaccgcg gttaatctgc aaggacacat
     2461 tgttgagcgc gtgaacatca ccgttaaaac cggggaaact caaatgcagt tgttgaatgt
     2521 ccagaacggg ttgggtcatg actgctttcc tcctgctttc gggtcaagca gatcgcgaat
     2581 accatcacca aagagattaa acccgacggc ggtaagcaaa atcgctgccc ccggaaatgc
     2641 gcaataccac cactgatcga gtacatagtt gcgaccattc gctaccatcg cgccccattc
     2701 agcactcggt tgttgagcac ccaggccaat aaatcccaac gtggcggcca ttaaaatcgc
     2761 gctaccgata tccagcgatg cctgcacgat cagcggcggc agggaattac gtaaaatatg
     2821 ccagttgatc agatgccaac gagacgcgcc aaaggttttc gccgcctgaa cataggtata
     2881 ctggcgtaca actaatgctt gcccccgcgc caggcgcaca taaaacggaa ttcgcactat
     2941 agcaatagcc agcatggcgt taaacaaact cggcccgaga gcggcagcca gtgccattgt
     3001 cagtaccaac gaaggaatcg acagcataat gtccatgatg cgcatgatga tggcgtctgc
     3061 gcgtccgcca agcacaccgg atagacatcc gagtagcgaa ccaatcatac ccgcaatggc
     3121 gaccaccact aatccggcga gaattgattg ctgactgccg accagtacgc ggctaaacag
     3181 atcgcgtccc acttcatcgg tgccaaacca gtgcgccgca gaaggcggca aaaggcgggc
     3241 ggttaaatca atggcgttgg gatcatgcgt cgccagccac ggtgaaaaaa tcatcatcag
     3301 tagcattaat acaatgatga ctgcgccggt cacggtgagc ggactgcctt tcagcatcca
     3361 taccagtttt gcaccgttaa atcgcgtttg tttttgtggg cgtacggcgg acgtttcctc
     3421 gcttagcatc attcaccacc tccacgtccg atacgcggat caatccacaa atagagcaaa
     3481 tccaccacca ggttgaccag cacataagca aatgaaacca cgacggcaaa gcccatcact
     3541 gccgggaagt cgagcgcctg tattgatgtt actacccatg cacccattcc gggccaggca
     3601 aaaacggttt cggtgagcac tgcgccatac aacaaatcgc ccagcgccag acccaatacg
     3661 gtaatcgatg ggatcaacgc attgggtagc gcataacata aaacgatata ccagccgggc
     3721 aagccgctgg cccgggcggt acgaatgtag tcttcactca attgttccag catcgctgag
     3781 cggatctggc gagcgacaat tcccaggtga acgaacgcca gcgttaatgc cggtaagatg
     3841 agatgttgca gcgcattgaa gaagacttca ccgttacctt caagcagcgc atcgagcaga
     3901 taaaagccgg taacgtgcgt tggtggatcc agccagtcat caagccttcc gccgccggga
     3961 agaatttgca gatgaccata aaacagcaca atgacgccca gcccaagcca gaacgcaggt
     4021 gtggagattc cggtaatggc cattattcgc accagatgat ccagccagcg atttcgccag
     4081 actgcagaga gtatgcccag cggaatgcca atcaggagtg ccagcagcag ggcataaaaa
     4141 gccagctcca gcgtcgccgg gaaaaatata cgcaactctt ccagcaccgg acgcccggta
     4201 cgaatggatg ttcccaggtc accatgaaac aggtcgctga cgtagcggta aaactgtaca
     4261 tacagtggct ggtccagtcc cagttgctgg cgaatatttt ccacgatagc atcgctggcg
     4321 cggtcacccg ccagtaaccg cgccggatca ccagggatca gatgcgagat aataaacgta
     4381 atcacgcaga cgcccgccac cacgagcacc agcccccagc agcgttggcg taaaatactc
     4441 cagaaggtca cgctatttac tcatggtatt gatattgaag acctgttcca gcatgggatt
     4501 gaacacaaag cctttcacct ctttgttcat cgccagttgg tagtttttct ggaacaggta
     4561 cacataagca gcgtcattaa tgacgatttt ctgtgcctgc tggtagtccc gggtacgctg
     4621 cgtctggtcg gtggtcgcga gtgcattgcg cagtaactta tcgacctcac tgttttcata
     4681 gaacgagcgg ttacccggca gacctttttt gtctgactca aaccagtaat tcataaacat
     4741 atacggatcg gcaaaatccg gactccagtt gccaatcgca atgtcgtaat cacctttacc
     4801 cactctgtcg cgcatggtgg cgttcgccag cttttccagc ttcacattga tgcccagctt
     4861 gttgagactg gattgtgtcg ccagagcaat aggctcccag ttcggatcat tatcagaata
     4921 gagaaacgtc aggctggtgg gtttgttcgt cactttatcc cattcagctt tggctttcgt
     4981 ttcgtcatgg ttgtattgca ttgccgtcgc atcgtagccc cacatgcctt ccggaatcgg
     5041 gccgcgcatc tgttttccgt taccactcag aatgccgtta accattccct gataatcggt
     5101 agaccaggaa atggcccgac gcagatccgc ctgattaagt ggcgctttgc tgttattcag
     5161 atacaggtag gtgacgcgca gtgacggata ctctgccaca ttgactttgt tttcctgctt
     5221 cagggcgttg agttgatcca ccggcagcgc atcggcaatg tcaatatcac cacgggagag
     5281 ctgcaggcga cgggaggcac tttcaccaat aatttttacc gatactcgct taaagttcgg
     5341 tttattgcca gggtaatgcg gatttggcac cagaactaat tgctgacctt tttgccagct
     5401 tttcagcata aatggtccgg aaccggcggt attttgcgcg aggaagccgc gggcatcatc
     5461 cgctgcatgt tcctttaaca ccgccggatt gataatggat gcaccgtcat tcgccagcgt
     5521 gtagaggaac ggtgcgaatg gctggctaag ggtaaacttc accgtatgtt cgtcgggagc
     5581 atcaatcttt aaatctttgg gaaatgcttc tgctggcccc tggccgattt ttagtagccg
     5641 ctcaaaagaa agttttactg cttcggcagt gacaggtgtg ccatcggcaa atttagcatt
     5701 atctttcagg gtgaacgtcc actctttttg atcgtcagac gctttccagc tacttgccag
     5761 atcgccttca acgtcggttg agcctttatc accgtccgtt ttgtactgaa ccagccgctg
     5821 ataagacggg taggtcactg tccagtcgtt attatctatt gttaccgccg ggtcgagggt
     5881 ttgtggatcg gcggccttac caatcaccag catatctttc ggtacggcgg cgtgcgcaac
     5941 cgggaaattt gtggcaagga cgagcgcgag caatgtggga cgaaacggta tcgatctctt
     6001 catgacagtt tcctgattaa ctgacgtgct gtgtgccagg ggatataaaa caagagaatt
     6061 gatcagcgag cagagggtaa ctcgctgcct ggggtaattc gaagtgccac cattcgctgg
     6121 agataccgac aaaaccgcca ccagtcatta tcgcattcag caacagccga ttgcgctgag
     6181 cggcgggcgg gacggaaggg tgataggcat gggagcgctc gtgcatttca tcgaaccctg
     6241 cgcccatatc gaggatgttc ccgtgctcat cacgaagcgt caggtcgatc gccgtgccac
     6301 ggctgtgatt agaaccgacc gtcacatcaa caacatattg cgggtctggg caggcttgcc
     6361 acaacatcgc ctgtgcttgt tgtgggcgat acgcatcgta aatcaccagt tgtaagcctg
     6421 acagctgggc gatactgata cttttcgcca gcgcggtaat cgcatccttg tgtagcagac
     6481 aacgcgcttg ctgataaata gctttacctg tgatgttatc agcgcaggcg tatttcaatt
     6541 cgatctccag atcagggaag attacggcta aatcaaccag ttcggtggta tccgacataa
     6601 acggttcctg tataagacaa aaattgctgc gctttccgct tatgcagatc tcatgccatg
     6661 ccgggataag cgccagaatg ctggcttaaa gttgtatttt ttaacttttg atcaacattt
     6721 gtgcagcgta gtgcagtttt ggtgcaagag gggaagttaa ggaaggaatc tcccggaatc
     6781 gtagctgaaa tcacagtatt taagtgacag tgtcacgtta aatgaaaacc cgcgagtgcg
     6841 ggcgagagga atttgtcaga ttttcagcgg taacacgctg ctcatccagc ctggaatttc
     6901 ttcggcgggt agggggcggg aaaagaaata tccctgaata acgcgacagt ggatcttgcg
     6961 tagcatctca aattgctctt tggtttcgac gccttccgcc acgacggtta aattgaggct
     7021 ttgcccaatg ctggtaatgg cttcaagtaa ggcaaggatg cgtttttcgg tcagacaacg
     7081 atcgacaaaa cttttgtcaa ttttgatttc cgttaccgga agactgacta agcgggataa
     7141 tccggaaaag cccgtaccaa aatcatctac cgataagccc acgcccatat cacgcaggat
     7201 ctgaatgcgc ttaaagattt cggtatcgtg ttccatcatc atgctttccg tgatttctac
     7261 cgtcagctgg tggccgtcaa taccccaggc gtgcattgca tcagacacct gattaggcag
     7321 ttgattactg cgaaagtgca gtgccgacaa gttaacggaa agcgccggga tatgaatatt
     7381 ctggctacgc cattctgcta actgacggca agcttccgcg atgacccagc gcccaatatt
     7441 ttcgatttca ccaatctctt ctgcgagagg aataaaccgt gaagggggca catgaccatg
     7501 ctggggatcg tgccagcgcg caagggcttc gatgccgtac agttcacccg tttctgcgaa
     7561 gatttgcggc tggtaaacaa gtttcagttg gttattgcta atcgcttctt tcagcgctgc
     7621 gcctaaaacc aaacgctctt ttaccatttc gtccatcgcc gggctgaaga actgccagcc
     7681 gttaccgcca ttcttgcgaa tataatccat tgcattgtga gcagtggaga gtaggtaatc
     7741 gcggttttta cccacgtcgt agctgatgcc aatactcaag gtaagcggga atggtttatc
     7801 gtcaatcatt atcggcttgc tgaccacatt ccgtagctca tcggcgatct gggtaatgtt
     7861 actgacgtcg ttttccaggc tcacgaggac aaactgcgta ccttcgatac gacagagata
     7921 ctgatccggt ttgagttttt cacgaaagcg attgaccact tccagcaatg cctgatcggc
     7981 ccacgcatag ccaaggctat caatcacatc ctgaatatgg tcaacaccga tgagatacac
     8041 catcggagag acggctttgt cgaccaggtc atcgaggtaa ttgtgcaggt tattgcgatt
     8101 tggcagaccg gtcatcggat caaattggat gagttgttca atatgctgac ggcttttttc
     8161 ctgttccagc gccagcgcgg ccatatgctg gctgatatct gccacgcgtt cgataaaggc
     8221 gctggtttct gctcctgacg aggttttaat ttgcaggatc cccgcaggcg cgccatcacg
     8281 ctgacgaatg gtcgctgacc agctttgcgc attttgagct tctgcaccgt gggaagatga
     8341 cgcccagtgt atcggcatcc cgttgcgcag tgcgaacagc gaaacatgcg attcgttgag
     8401 tacagattcg atgttacgac aaatgatttc ccccatttca tgaaatggcg ggctgctgca
     8461 catggcggcg agaatattgc cttcaagctg gcgaatctgc cgttcttcgg tgatatccga
     8521 gaaagtcatt accaggttct gcagatgcgc gagcacgtca taaaccgggc tgatagaggc
     8581 tttaatccag attttttcac cggtgcgcgt caacagcaga aattcgtcct gatcacgggc
     8641 ggttttccat agcaactgtt gtaaacgaat gcggttatcg gcagggaatt caggaatgtt
     8701 caggagtgta tcgggctgca taccgctggc ttcgctaatg cagtaaccaa acatttcggt
     8761 gaatgcgcga ttgcactgca caatatggcg ttccggatcg aggacaatca ccggtcggtc
     8821 gagatggtca acggcaataa tcaattgtcg ggtctgttct ttttgcgcca tttctacgct
     8881 ggcatcccgt accagcgcca ggtaataaac tttcccctcg gcgctcactt tcgatagcgc
     8941 aaaacgggtc cagattttac tgccgtcttt tttctccagc tgcagctccc gactcatccc
     9001 ctcgacgcgc gctttaccgc cttcacggtt atgacgaata tattcaggat gcgcaggacg
     9061 caaatcccgc ggaatcagca tatcaatgtt attgccaatg acttcttcac gtttgtaccc
     9121 ccagagcttc tctgcggcgg ggttgaaaaa catcacttca tcattttcgt taattaacac
     9181 cgcacccatc atattttgct caagggcggg gaaaaaaatg ccatcggcgg cattttccgc
     9241 atcggttagc ttcatgatta cctctgcatc ctggcgcatc taaagactgg ctttccagag
     9301 ttcaacacgg tttctacctc gtcttttggc gatatacaga gcttcatcgg ctatttgaat
     9361 gaggcgctca tagtcaggat gaccattaaa catggcggca ccgatggaaa gtgagagggc
     9421 aatatcctcg ccgtttgcgg ctttcagttt ggttttctcc acccgactgc gaatacgttc
     9481 tgcggtacgt aacgtttcgt tttccgaagc ttcagtcaaa acaatgataa attcatcgcc
     9541 cccgtagcga aaaacataat cactactgcg gacgttgtca taaaaggcct gagagacttt
     9601 acgcagaatt tcatcgccag tgttatggcc ccacgtatcg ttgatctctt tgaatttatc
     9661 aacgtcaata atcagcactg acagcggtgt accggtccgg ttggcatggg caatttcgcg
     9721 tttgaagata gtcggcagga agcggcggtt aagtaatttc gtcagtacat ccataccgac
     9781 ttcgtggcgc ga
//
LOCUS       DAJSKJ010000145         9769 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000145 DAJSKJ010000000
VERSION     DAJSKJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.146"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..1274)
                     /locus_tag="ON345_003049"
     CDS             complement(<1..1274)
                     /locus_tag="ON345_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HCR3073075.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLT"
     gene            complement(1443..2321)
                     /gene="glxR"
                     /locus_tag="ON345_003050"
     CDS             complement(1443..2321)
                     /gene="glxR"
                     /locus_tag="ON345_003050"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR3073076.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(2421..3197)
                     /gene="hyi"
                     /locus_tag="ON345_003051"
     CDS             complement(2421..3197)
                     /gene="hyi"
                     /locus_tag="ON345_003051"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR3073077.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(3210..4991)
                     /gene="gcl"
                     /locus_tag="ON345_003052"
     CDS             complement(3210..4991)
                     /gene="gcl"
                     /locus_tag="ON345_003052"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR3073078.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAKRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(5081..5896)
                     /gene="allR"
                     /locus_tag="ON345_003053"
     CDS             complement(5081..5896)
                     /gene="allR"
                     /locus_tag="ON345_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR3073079.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(5974..6456)
                     /gene="allA"
                     /locus_tag="ON345_003054"
     CDS             complement(5974..6456)
                     /gene="allA"
                     /locus_tag="ON345_003054"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR3073080.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            6686..7612
                     /gene="allS"
                     /locus_tag="ON345_003055"
     CDS             6686..7612
                     /gene="allS"
                     /locus_tag="ON345_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR3073081.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LEPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            7681..8775
                     /gene="mnmH"
                     /locus_tag="ON345_003056"
     CDS             7681..8775
                     /gene="mnmH"
                     /locus_tag="ON345_003056"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR3073082.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQAIIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            8891..9256
                     /locus_tag="ON345_003057"
                     /pseudo
     CDS             8891..9256
                     /locus_tag="ON345_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2564 a         2538 c         2294 g         2373 t
ORIGIN      
        1 gtgaggttaa aaccgttatc gtaatattta taatcgccag gtgcggtata cagttcatca
       61 agattaattt tcccacgcat caccacaaaa taatgcgcca tcatgacacc aattaccgga
      121 ccaagcattc cgccgataat atcgaggaac agataaatac tgtcctgatt ttccattaat
      181 ttccacgggc agatcagcag gctgataata ctggcaatca gtacgccgtt tttataagtc
      241 agtttcgtcg gtgcaatagc ggcaatctga taaccggcag gaataatatt accggtggcg
      301 ttagtggaga tagtcgtcat cagaataacc agtaccgcaa agaacgaggc gaacaagctg
      361 tcccaacgct gaacaatatc cagcacgttc caggtatcag cgccgtaatg aatactggct
      421 ccggcaataa tacatacccc cgcgaccgca aacagaatat aggccacaac taaacccagc
      481 gtttgcccca gcgcttgctc acgaaacgag tgggcgtttt gcgtaaagtc ggatgcgctc
      541 accgccggtg ccgcccagac cgcaactacc gcgtttatca ccaccaggaa caggaagcca
      601 ctgttttctg ctttctgaat accgctcgga atgtagtcaa agattggacc gagcccgacc
      661 agcgaaatcg cccaaatcgc cataccgccg aaaacgatat agatgcacgg gttaagaatg
      721 gcagtgaatt tatttaaaac tttgccaccg ccaaaaccta taccaacgtt gaccagccag
      781 aagagtaaga aagtaattaa gcccggtaga gaaagcccta acagcgtgaa atcaccaccg
      841 agagttaaaa atcccggcca gattttgcca atcagaatca agcaggccag tgaccccgcg
      901 taacattgca ggccaaacca cataatggca gcaataccgc ctcttaataa tccgggaaac
      961 agtgcgccac gtacgccgta agaagcacgc aggatcatgg caaacggtac gccgtattta
     1021 ctgcccgcag cgccgtttaa taccattacc gcggcaatga aaaaggcgct gaggataatt
     1081 gccagcataa tactaaaggt agacaagccg agaataaaaa agccgccgac catcacataa
     1141 ttgggaacgt tatgaaccga acccatccat aaggtaaaat agttaaatgt tttccaggtc
     1201 cgctggcttt gcgttttcgg caatagatct tcgctatagc cgcgttgctg gaatagtttt
     1261 ctctgatgtt ccataaatcc ctcttaattt cacgtatcga ataacgttgt tttaatctag
     1321 ctatgaggaa caaataaaat aaaaatatta tttttaatac tgaagcaaca tctttggctg
     1381 ctttcttttt taatattgct ggccgattca ttttctgata tcggccattt ttattgcggg
     1441 tatcaggcca gtttatggtt agccattaat tccagcgcct gcactaacgc agagtgatcc
     1501 aactggctgc caccgtttgc cgcacaggta ttaaataact cctggcaggt cgcagtgttt
     1561 ggcaggttca gcgcaagtgc tttcgcactt tgcagtgcca ggttgagatc tttctggtgc
     1621 agagcgattt tgaagcccgg attaaaggtg cgtttaatca tacgctcgcc atgaacttcc
     1681 agaatacgtg aggaagcaaa gccgcccatc agcgcctggc gcacacgtac cgggtccgca
     1741 ccggcttttg aagcaaatag cagggcttca gaaaccgctt caatattgag cgccacaata
     1801 atctgatttg ccactttgca ggtttgaccg tcgccgttac cgcccacgag ggtgatattt
     1861 ttaccgagca gttcaaacag cggtttaaca cgttcaaata ccgcttcatc accgccaacc
     1921 ataatcgaca acgtcccttc acgcgcaccg atttcaccgc cggagactgg cgcatcgaga
     1981 taatcgccgc ccagttcatt cacctgacga gcgaaacgct tagtttcaat cggggaaatg
     2041 gagctcatat caacaatggt tttgcccttc agcgaggctt tggtacaacc attttcaccg
     2101 aacagaactt cttcaacctg aggtgtgtcc ggcaccataa taaaaatgat gtccgatgct
     2161 tccgttacct ggcgagcagt ttcaacactg acggcaccca gtgacagtaa ttcatcagca
     2221 accggtccaa tggtcgtgac atgtaattga tgaccggcac gcgccagatt aatggccatc
     2281 ggtgtaccca taatgcctaa gccaataaat cccagtttca taattaacct cttttaaatt
     2341 tcgcttttcc tgaattcaga caacacgatc ccctgccaac ttagcggcct aaaaaagcat
     2401 tgtctgaata gcgttacgtt ttaacggtac ggatccatcc agcgtaaacc ggcttccgtg
     2461 gtggtttggg gtttatattc acacccaacc caaccgttgt agtcagaatt ttcgattacc
     2521 ttaaagagat aatcataatt aatttctccg gttcccggtt cgccgcgatg cggattatcg
     2581 gcaatttgca ggtgaccaat tttatcagcc cactgagtca tggtgttggt taattcacct
     2641 tccatccgct gcatgtgata aatgtcatac tgaattttca aattgcagca accaacatca
     2701 tcaatcaatt tcagcgcctg ccgagttccg gtgagatgga aaccaggaat atcaaaatgg
     2761 ttaataggtt caatcagtaa taaaatatct tctttcatca gcatattcgc ggcataacgc
     2821 aggttttcta caagcgttgc gtgaatctgt tcactgctga aaccagccgg cgttttaccg
     2881 accagacagt taattttttt attacccagc gcacgggcat aacgaatcgc tgctgctacg
     2941 ccatcccgaa actcttcttc acggccagga atacaggcaa taccgcgctc ccccgccgcc
     3001 cagtcacccg ccggtaaatt gtgcagcgta tgttcgagtt tattactcgc cagcacctgt
     3061 tttaattctt caatgtcgta gtcataagga aacataaatt caacgccgcg aaaaccacac
     3121 tgcgcagctt tctcaaaacg ggcgagaaaa tcatattctc caaataacat cgataaatta
     3181 gcagagaaac gtaacattat ttatctccct tattcatagt gcatgaagca ggtttcagtc
     3241 ggtgcgtccg ctgcgttatc ggcgatatct tcaaattcca tgacgttatc cagttcgctg
     3301 cccatcgaaa tattggtcac acgctcgaga ataacttcca cgactaccgg tacccgatat
     3361 tgcgccatta aggctttcgc ctgttcaaag gctggcgcaa tatcttccgg tttaaagacg
     3421 cgaatcgctt tacaacctaa accttccgct acttttacgt ggtcgacgcc gtaaccgttc
     3481 acttcgctgg agttgatatt ctcgaaagcg agttgcacgc agtagtccat atcaaacgcg
     3541 cgctgcgact ggcgaatcag ccccagataa gcgttgttga ccagcacatg gatgtacgga
     3601 atgttgaact gcgcgccaac agctaactct tcaatcagga actggaagtc aaagtcgcca
     3661 gaaatcgcca ccacattgcg tttcggatca gcggcacaaa cccctagcgc agccggaatc
     3721 gtccagccta acggaccagc ctgaccacag ttgatccagt ggcggtcttt aaagacatgc
     3781 agcatttgtg ccgcagcgat ttgtgacaga ccaatggtgg tgacataaca aacatcgcga
     3841 ccaaaggctt tgttcatctc ttcatacacg cgctgcggtt tcaccggcac gttgtcgaag
     3901 tgggttttgc gcagcaaagt gcgtttacgc tgctggcagt cggcgaccca ttctttacga
     3961 cacggcagac gacccgcttt ttgcatctcc tgcgccactt caaccagcag tgtcagcgcc
     4021 gctttagcat cagagacaat gccgagatcc ggacacagca cgcgaccaat ttgcgtcggc
     4081 tcaatatcga tatgaacgat tttgcgccct tcggtgtatt tctctaccga accggtatga
     4141 cggttagcaa aacggttacc gataccaaac accatgtcgg acgccagcaa cgttgcgtta
     4201 ccgtaacgat gcgcggtttg cagacccacc atcccggcca tcagttcatg atcgtccggg
     4261 atacagcccc agcccatcag cgttgggatc accggaacgc tggtcagttc agcaaactgt
     4321 tgtaacagtg cagctgcgtc agcattaatt accccgcccc cggcaacaat caccggacgt
     4381 ttggcctgga ttaacatttc tacagctttt tcgatctgca tacggctggc agcaggtttg
     4441 tagaccggca gcggttcgta catatcagga tcaaactcga tttccgcaac ctgaacgtcg
     4501 aacggtaaat ccaccagtac cggacccgga cgaccagaac gcatcaggtg aaatgcctgt
     4561 tgcagcacac gaggcaccag cgccgcttca cgtactgtaa ccgccatttt gctgaccggc
     4621 ttggcgatag cttcgatatc aacagcctgg aaatcttctt tatgcagacg ggcgcgcgga
     4681 gcctggccgg taatacacag aataggaatg gaatcagcag aagcagaata gagcgcggtg
     4741 atcatgtccg tgcccgcagg accggaagtc cccagacata cgccgatatt ccctgccgtt
     4801 gcgcgggtat aaccttccgc catgtgcgaa gcaccttcca catgacgcgc cagaatgtga
     4861 cgaataccgc cgtgcttacg catcgctgag tagaacggat tgattgcagc tcccggaacg
     4921 ccgaaggcgg tggtgatacc ttctttctcc agcacataca ttgccgcgtc aacggctctc
     4981 atttttgcca ttttattcct acctctatta attggaaaat ttttccaact ttcaaatttt
     5041 gtcaaaaaaa cccgctccgc agagcgggat gcgacagaca ttatggatgt gctttcagtc
     5101 ccaacgccgt gctgatatcg cgggcggtgt ctctgaccag ctcaccctga ctgacaaaac
     5161 gatcttctgt cagtcttgat gaaggcccgg agatggagat agcggcaaca acactaccta
     5221 catcatcgta aattgctgaa gctaagcaat tcagacctac aacatgctct tctttatcta
     5281 cggtatagcc cagttcacgc gcttgttcca ggtccttcag caaggtgggc atatccacaa
     5341 gcgtagttgg cgtaaactgc tgcaaaccgg tttgcagaat gatgctcatc aactcctctt
     5401 ccgccagcgg ataaagcagc gctttgcccg caccggaagc atgcagcggc agacgactgc
     5461 ccagtggcgc acacatcctg accatcgatt tacactctaa ctgaccaatt aatactgctt
     5521 cattgccgtt acggatcgcg acattgaccg tttcgccgga aagtaacatc aggcggcgca
     5581 taaacggccc ggcgacggag aggacatcgc ggttatggat gtacgctgca ccgacgttaa
     5641 agacacctaa tcctatatgc caccagccta actgactgtc ctgatagaca aaatccgctg
     5701 cctgtaaaac cttcagcaag cgaaaggtcg tggagagcgg caaatccaga ttgagagaaa
     5761 tatcgctaac cgacgagctt cccccacttt tttccagata ttgcagaatc gcaattcccc
     5821 gctctaacgc ctgtgcgccc ttctgtgcca caggctccgc ctgtcctggc ctgccgcgcc
     5881 gtctaacttc cgtcatacct acgttaactc cgacctacaa gtgagcataa acaatgaaca
     5941 attagtttat cttatgcaag tcggttgcag gcgtcacgca aaacagagtt cctgttcagg
     6001 aatactttca acatcacagt tgtcactgcc gccgcgatcg atggtcagaa aatcggtgac
     6061 gcgctgccag gcgaaaagtg ggtgatgcca gacgttacga tggtaattca ctccctgttc
     6121 gccgttggtg ataaacgccc gcagcgttga caggtctggt ttgtcgtcgc ctaacgccac
     6181 gaccaccaca aacacttcac ctttcatcgg gataaaggcc tgagtgccca gcggatgacg
     6241 ttcgagttcg tgaatggtca gcggcagatt cgccggttgc gcgcggttaa tgctgataag
     6301 cgtacggtct tgctcaagaa tctcaaccag cgccaaatcg tggtaacgct ccaccaggcc
     6361 attgttaata tggaaaaaat cccgttgctg agtttcgatt acgtcgccat aagcactaaa
     6421 ggcttcctga cttaacggta atacctgaag tttcatcttt ttgtccttgt aacataattc
     6481 cgttcaacca ccgccgaccg gtcagcttgg tttcaataac aggcaatcta aaaccttaag
     6541 agaacaacat caatacaaaa gttggaatat ttttccaaaa ctagatttag tccacagatt
     6601 gaacagggtt atcatgtaaa aatactgata aatcagagga taagaaaatc acgcaacttg
     6661 cggatttaaa aggggatacc tgaagatgtt cgatccagaa accttgcgga ctttcattgc
     6721 ggttgctgaa acaggaagtt tttcaaaagc ggcagaacga ttatgtaaaa ccacggcgac
     6781 gatcagttat cgcattaaac ttctggaaga gaataccgga gtagcgctgt ttttccgtac
     6841 gactcgtagc gtaacgttga cagcggctgg cgagcatcta ctttcccaag ccagagactg
     6901 gctgagctgg ctggaaagta tgcccagcga gctgcaacag gtgaatgatg gcgtggaacg
     6961 ccaggtgaat attgtcatca acaacctgct ctataacccc caggccgtcg cccagttgct
     7021 ggcgtggctg aatgaacgtt acccctttac ccagtttcac atctcccgac aaatctatat
     7081 gggcgtctgg gactcgctat tgtacgaagg tttttcgctg gctatcggcg tcacgggaac
     7141 tgaggcgctg gcaaatacct ttagtcttga acccttagga tcggtgcaat ggcgatttgt
     7201 catggcggcg gatcatccgc tggcgaacgt tgaagagccg ctaacagaag cgcagttgcg
     7261 tcgctttccg gcggtcaata ttgaagacag cgcccgcacc ttaaccaaac gcgtcgcctg
     7321 gcgattgcca gggcaaaaag agattattgt tcctgatatg gaaacgaaaa tcgccgccca
     7381 tcttgcgggc gttggcattg gttttttgcc aaaatcgctt tgccagtcaa tgatcgataa
     7441 tcaacaactg gttagccggg taatcccaac gatgcgccct ccttcgccat tgagtctggc
     7501 atggcgcaaa tttggcagcg gcaaagcggt agaagatatt gtgaccttgt ttacccagcg
     7561 caggccggaa atcagcggat ttttagaaat tttcggcaac ccacgcagtt aaaaatcaag
     7621 tttccttgcg cgttggcgcg taaaatagcg cgcaagctga tttgaggttc acttttaact
     7681 atgcaagaga gacacacgga acaggactat cgtgccctgc tgattgctga tacgcccatt
     7741 attgatgttc gcgcccctat cgagtttgag caaggcgcaa tgcccgccgc tatcaatctg
     7801 ccgttaatga ataacgatga acgcgccgcc gttggcacct gctataaaca gcaaggctca
     7861 gacgcagcgc tggcgctggg gcataaactg gtggcgggtg aaattcgtca gcagcgcatg
     7921 gacgcctggc gggcagcgtg cctgcaaaat ccgcaaggta ttctctgctg cgcccgtggc
     7981 ggtcaacgtt cacatattgt gcaacgctgg ctgcatgaag cggggattga ttatccgctg
     8041 gtggaaggcg gttataaggc actgcgccag accgcgattc aggcgatcat tgaactgtca
     8101 caaaaaccga tagtgctgat tggcggttgt accggcagcg gtaaaacgct attagtgcaa
     8161 caacagccaa acggtgttga tctggaaggg ctggcgcgcc atcgcggttc ggcgtttggt
     8221 cgcacgttac aaccacaact tagccaggcg agttttgaaa acctgctggc tgccgaaatg
     8281 ctgaaaaccg acgcccgtca ggatttgcgc ctgtgggtgc tggaagacga aagccggatg
     8341 atcggttcga atcacctgcc ggaatgcctg cgcgagcgaa tgactcaggc ggcgattgcg
     8401 gtggtagaag atccgtttga gatccgtctt gagcgcctga acgaagagta tttcttgcgt
     8461 atgcatcatg attttaccca cgcttatggc gacgaacagg gctggcagga atattgcgaa
     8521 tacctgcatc acggactttc ggcgattaag cgtcggctgg ggttacagcg ctataacgaa
     8581 ctcgctgccc agctggatac ggcgctgaca acgcaactca ccaccggcag caccgacggt
     8641 catctggcct ggctggtgcc gttacttaaa gaatattacg acccaatgta tcgctatcag
     8701 cttgagaaaa aagcggaaaa agttgtcttt cgcggtgagt gggcggaagt ggcggaatgg
     8761 gttaaggcgc ggtaatgagg gacgtatttg tattagtcgc gacttgatct gacacatggc
     8821 cttgaaaggt tgagagttac cggttttgat atgagtgtcg aatccttatc caaaacaaga
     8881 ggtaactctc atgcttcata ctgccaatcc cgtcatcaaa cacaaagccg gtttgctcaa
     8941 tctggctgaa gaactcagca acgtatcaaa agcctgtaaa atcatgggcg tttcgcgtga
     9001 tacgttttat cgttaccgcg aactggtcgc tgaaggcggt gtggatgcgc agattaatcg
     9061 tagtcgccgc gctcctaacc ttaagaaccg taccgatgag gcaactgaac aggctgttgt
     9121 tgattacgcc gtcgctttcc cgacacatgg tcagcaccgg gccagcaacg agctgcgtaa
     9181 acagggggtt tttatctccg acagtggtgt ccgttctgtc tggttactcc ataaccttga
     9241 gaatctcaaa agacgatact gaacaaattt tatcaggtga cgttccgtaa aaagttgtat
     9301 ggagttctcg atgcagtcgg cgcgactatc agtacccccg ctgaaccctg gactatcggg
     9361 ccgccgtact ggggcatgtc accctggcgt gccgctggtt ttccgctaat ggtttatttc
     9421 ctctttcttt cccggcaaga atttatttta atccggaagg attatttatt ttcagatagg
     9481 gactcacatt aaacacgtaa cattttaatt aatgcatggc tgaatatgta ttacacaatg
     9541 tcagatatcg taaaagacca gtgcaattct atgtaaactc tctgactgtt cattttattt
     9601 gttgtttcag ggtcggaact caccctcaat aaacaactga atacgagtag atattcatcg
     9661 tctgagctat atggctttac acaatagccg acatgtcttt tctcagtcat gaattttact
     9721 tttcctcact acgaagttga cgacttcgat atgggataga ctcttaatt
//
LOCUS       DAJSKJ010000146         9687 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000146 DAJSKJ010000000
VERSION     DAJSKJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.147"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            83..2344
                     /locus_tag="ON345_003058"
     CDS             83..2344
                     /locus_tag="ON345_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR3073083.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2578..3861)
                     /locus_tag="ON345_003059"
     CDS             complement(2578..3861)
                     /locus_tag="ON345_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR3073084.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4013..4489)
                     /locus_tag="ON345_003060"
     CDS             complement(4013..4489)
                     /locus_tag="ON345_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR3073085.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(4548..5837)
                     /gene="bioA"
                     /locus_tag="ON345_003061"
     CDS             complement(4548..5837)
                     /gene="bioA"
                     /locus_tag="ON345_003061"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR3073086.1"
                     /translation="MTTDDLAFDQHHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            5924..6964
                     /gene="bioB"
                     /locus_tag="ON345_003062"
     CDS             5924..6964
                     /gene="bioB"
                     /locus_tag="ON345_003062"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR3073087.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            6961..8115
                     /gene="bioF"
                     /locus_tag="ON345_003063"
     CDS             6961..8115
                     /gene="bioF"
                     /locus_tag="ON345_003063"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR3073088.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            8102..8857
                     /gene="bioC"
                     /locus_tag="ON345_003064"
     CDS             8102..8857
                     /gene="bioC"
                     /locus_tag="ON345_003064"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR3073089.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            8850..9527
                     /gene="bioD"
                     /locus_tag="ON345_003065"
     CDS             8850..9527
                     /gene="bioD"
                     /locus_tag="ON345_003065"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR3073090.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYITTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         2304 a         2528 c         2672 g         2183 t
ORIGIN      
        1 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
       61 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
      121 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagccaa
      181 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
      241 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
      301 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
      361 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
      421 ggcagcgcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
      481 tatgcgtgag atgatggcag gcggcggcaa aatgatcctc gggtcagaca gccacacccg
      541 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
      601 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
      661 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
      721 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
      781 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
      841 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gccaggatta
      901 ctgccagctt aatcctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
      961 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     1021 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     1081 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     1141 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     1201 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcagttt acccgtcatc
     1261 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     1321 cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata cgccaatcaa
     1381 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     1441 agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     1501 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     1561 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     1621 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     1681 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtga ccaccaccga
     1741 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcgga
     1801 gtttaccctg tcacgccgcg atcccggtta tgttggcaga agtaaagcga ctgctgagct
     1861 ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa
     1921 gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca gcatggtata
     1981 tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc agcgtgtgat
     2041 tggcggtctg gcgaatattg ccgaagagta cgcgactaaa cgctaccgtt ctaacgtcat
     2101 caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag tgggggatta
     2161 catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt ttaaaggtta
     2221 tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa gtctgactgc
     2281 tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa accgtcagat
     2341 gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat gcgatgcttg
     2401 cgcatcttat ccgacctacg aatcgcatcg aatctgtagg ccagataagg cattttcgca
     2461 gcatccggca cttattgtcg gatgcgatgc ttgcgcatct tatccgacct acaaatcgca
     2521 tcgaaccgta ggccggataa ggcgtttacg ccgcatccgg caaatagtta attgctctta
     2581 cttcttcgcc tctgcaacca ctttactacc cacgccgcgg ttattgtatt cccacatgcg
     2641 gttgtagtta gtgtcattca gattgcgctg tatttcgtcg ttatcatcta cgctgccggt
     2701 attacccgca aacggacgat tagagatcac cgcatcggcc cacggtttag ccgtgttaaa
     2761 accttcgttg atggcgctat cacggatcac cacctgaccg ttggtattgg catcaacatc
     2821 cagcgagcgg cccagttgcg ccacaccatc accgaaagca ttgaaacggc tgtttacggc
     2881 gaggaaaccg tagtaaatgt tggacagcgt agccggtgca aacacatacg cttcttgctg
     2941 agtacgtgag ttcaccacgc ggaattcggt gttatcgaac accactgcgc cgcgaccaga
     3001 aacgatatcc acatcccctt caatgtagct gttggtcacc agcgtacgcg gctgacgatt
     3061 cgtttccaga cggttctgca caccgctgtt ggtgacaaag aaggtgttct gacgaccgag
     3121 aatgttaacg ttgttaatct gtacctggtc accatcagta cgcagtgcca ccgccggatg
     3181 gttacctgca tctacgctat cgcccagcgt gttttcgatg gtcagatttt gcagttgcag
     3241 gccattgttt tgtgaccaga agaccgcaga gcagagaaca ccgatactgt cgctgcgttt
     3301 gctctggcag ctatcgtaca tataccacgc tggtttacct ggcatatatt tgccgcgcgg
     3361 gttgacgtcg tgacgccagt cggcagggct catgccacca tcaagggaaa gcccaatctt
     3421 cacatcaatc ggtttttcac ctgtaccgta cagagtaatt ccacccggag cggcagggac
     3481 atataccgtt ccctgatact caccaggcat cacggcaata tactggcgct tgttggtacg
     3541 cttgataatt gccgcatcta ccgccgcctg aatcgtggta tgcgttacac cttgagtgcc
     3601 cgccgggccg acaacaaagt caggttgcgc aggcagggta atcggggaag gattccacgc
     3661 tgcagcacct ggtgtcaggg atgcaaaata gtgttgagca tcgaaattct gcgcttcttt
     3721 tgccgacaga atcgggcgag aagaggtacc aggcgcggtt tgatcagaag gacgttgatc
     3781 gggcggggtt gagctacagg cggtcagcgt cacgccaaaa gccaatgcca gcgccagacg
     3841 ggaaactgaa aatgtgttca caggttgctc cgggctatga aatagaaaaa tgaatccgtt
     3901 gaagcctgct tttttatact aacttgagcg aaacgggaag gtaaaaagac aaaaagttgt
     3961 ttttaatacc tttaagtgat accagatggc attgcgccat ctggcagagt gattaactaa
     4021 acatcgcagt aatcgaggcg cttgccagag agtggaaatg aacgttaaac ccgaccatcg
     4081 cgccgctggc accttcatcg acatcaatac gttctatatc cagcgcgtga acggtaaaaa
     4141 tgtagcgatg agtttcgcct ttcggcggtg ctgcgccatc gtacccggtt ttaccaaagt
     4201 cggtacgcgt ctgcaaaacg ccgtctggca ttgctaccag accagagcca aacccttgcg
     4261 gtaatacgcg ggtatcagcg ggtaagttaa caactaccca gtgccaccag ccggagccgg
     4321 ttggcgcatc cgggtcgtag caggtgacaa caaaactttt cgttcccgca ggaacatcat
     4381 cccacgccag atgcggtgaa atattatcgc catcgtaacc catgccgtta aagacatgac
     4441 gatgcggcaa tttatcgcca tcgcgcagat cgttactgat gagtttcatg aaccctcctt
     4501 tcttgtttgc agaaagtgta gccagaaacc ctcacgcgga cttctcgtta ttggcaaaaa
     4561 aatgtttcat cctgtaccgc gcggttaacc gctgcggtca gacgctgcaa ctgttgcggg
     4621 agaataatat agggcggcat caggtaaatc agtttgccaa aaggccggat ccagacaccc
     4681 tgttcgacaa agaatttttg cagcgccgcc atattcaccg gatgagtggt ttcgaccacg
     4741 ccaatggccc ccagtacgcg cacatcggca accatttcgg catcacgggc gggggcaagt
     4801 tgctcgcgca gctgtacttc aatatccgcc acctgttgct gccagtcgcc agattcgaga
     4861 atcgccaggc tggcgtttgc tgccgcgcag gccagcggat tgcccataaa agttggccca
     4921 tgcataaagc aaccggcttc accgttactg atggtttctg caacctcgcg cgtggtgagt
     4981 gtggcggaaa gggtcattgt gccgccggtt aaggctttac cgaggcacaa aatgtccggc
     5041 gcgatttctg catgttcaca ggcaaacagt ttcccggtac gaccaaatcc agtggcgatc
     5101 tcgtcggcaa tcagcaagat accttcgcga tcgcatattt tgcggattcg ttttaaccat
     5161 tccggatggt acatgcgcat cccgcctgcg ccctggacaa tcggctcaat gatcaccgcc
     5221 gcgatttcat gacgatgcgc cgccatcagg cgggcaaagc ccaccatatc gcgctcatcc
     5281 cattcgccat ccatacggct ttgtggggcg ggagcaaaca agttttctgg cagatagcct
     5341 ttccacaggc tgtgcattga gttgtccgga tcgcacaccg acatcgcgcc aaaggtatcg
     5401 ccatgataac cattgcggaa ggtcagaaaa cgctggcgcg cttcgccttt ggcttgccag
     5461 tactgcaacg ccattttcat cgccacttcc accgctacgg aaccggagtc cgcgagaaaa
     5521 acgcactcca gcggttgcgg cgtcatcgcc accagtttgc ggcacagctc aatggctggc
     5581 gcatgggtga taccgccaaa catcacatgc gacatggcat caatttgcga cttcatcgcc
     5641 gcattaagct gcgggtgatt gtagccgtgg atcgccgccc accaggacga cattccgtca
     5701 accaggcgtc tgccgtcaga caaaatcagc tcgcaacctt cggcgctcac caccggataa
     5761 accggcagcg gggaggtcat ggatgtgtat gggtgccaga tatggtgttg gtcaaaggca
     5821 agatcgtccg ttgtcataat cgacttgtaa accaaattga aaagatttag gtttacaagt
     5881 ctacaccgaa ttaacaacaa aaaacacgtt ttggagaagc cccatggctc accgcccacg
     5941 ctggaccttg tcgcaagtca cagaattatt tgaaaaaccg ttgctggatc tgctgtttga
     6001 agcgcagcag gtccatcgcc agcatttcga tcctcgtcag gtccaggtca gcacgttgct
     6061 gtcgattaag accggagctt gtccggaaga ttgcaaatat tgcccgcaaa gctcgcgcta
     6121 taaaaccggg ctggaagccg agcggttgat ggaagttgaa caggtgctgg agtcggcgcg
     6181 caaagcgaaa gcggcgggat cgacgcgttt ttgcatgggc gcggcgtgga agaatcccaa
     6241 cgaacgcgat atgccgtacc tggagcagat ggtgcagggg gtaaaagatt tagggctgga
     6301 ggcgtgtatg acgctgggca cgttgagtga atctcaggcg cagcgcctcg cgaacgccgg
     6361 gctggattac tacaaccaca acctggacac ctcgccggag ttttacggca atatcatcac
     6421 cacccgcact tatcaggaac gcctcgatac gctggaaaaa gtgcgcgatg ccgggatcaa
     6481 agtctgttct ggcggcattg tgggcttagg cgaaacggta aaagatcgcg ccggattatt
     6541 gctgcaactg gcaaacctgc cgacgccgcc ggaaagcgtg ccaatcaaca tgctggtgaa
     6601 ggtgaaaggc acgccgcttg ccgataacga tgatgtcgat gcctttgatt ttattcgcac
     6661 cattgcggtc gcgcggatca tgatgccaac ctcttacgtg cgcctttctg ccggacgcga
     6721 gcagatgaac gaacagactc aggcgatgtg ctttatggca ggcgcaaact cgattttcta
     6781 cggttgcaaa ctgctgacca cgccgaatcc ggaagaagat aaagacctgc aactgttccg
     6841 caaactgggg ctaaatccgc agcaaactgc cgtgctggca ggggataacg aacaacagca
     6901 acgtcttgaa caggcgctga tgaccccgga caccgacgaa tattacaacg cggcagcatt
     6961 atgagctggc aggagaaaat caacgcggcg ctcgatgcgc ggcgtgctgc cgatgccctg
     7021 cgtcgccgtt atccggtggc gcaaggagcc ggacgctggc tggtggcgga tgatcgccag
     7081 tatctgaact tttccagtaa cgattattta ggtttaagcc atcatccgca aattatccgt
     7141 gcctggcagc agggggcgga gcaatttggc atcggtagcg gcggctccgg tcacgtcagc
     7201 ggttatagcg tggtgcatca ggcactggaa gaagagctgg ccgagtggct tggctattcg
     7261 cgggcactgc tgtttatctc tggttttgcc gctaatcagg cagttattgc cgcgatgatg
     7321 gcgaaagagg accgtattgc tgccgaccgg cttagccatg cctcattgct ggaagctgcc
     7381 agtttaagcc cgtcgcagct tcgccgtttt gctcataacg atgtcactca tttggcgcga
     7441 ttgcttgctt ccccctgtcc ggggcagcaa ctggtggtga cagaaggcgt gttcagcatg
     7501 gacggcgata gtgcgccact ggcggaaatc cagcaggtaa cgcaacagca caatggctgg
     7561 ttgatggtcg atgatgccca cggcacgggc gttatcgggg agcaggggcg cggcagctgc
     7621 tggctgcaaa aggtaaaacc agaattgctg gtagtgactt ttggcaaagg atttggcgtc
     7681 agtggggctg cggtgctttg ctcaagtacg gtggcggatt atctgctgca attcgcccgc
     7741 caccttatct acagcaccag tatgccgccc gctcaggcgc aggcattacg tgcgtcactg
     7801 gcggtcattc gccgtgatga gggggatgca cggcgcgaaa aactggtgtc actcattgcg
     7861 cgttttcgtg ccggagtaca ggatttgccg tttacgctgg ctgattcatg cagcgccatc
     7921 cagccattga tcgtcggtga taacagccgt gcgttacaac tggcagaaaa actgcgccag
     7981 caaggctgct gggtcacggc gattcgcccg ccaaccgtac ccgctggtac tgcgcgactg
     8041 cgcttaacgc taaccgcagc gcatgaaatg caggatatcg accgtctgct ggaggtgctg
     8101 catggcaacg gttaataaac aagccattgc agcggcattt ggtcgggcag ccgcactcta
     8161 tgagcaacac gcagatctac agcgccagag tgctgacgtc ttactggcaa tgctcccaca
     8221 gcgtaaatac acccacgtac tggacgcggg ttgtggacct ggctggatga gccgccactg
     8281 gcgggaacgt cacgcgcagg tgacggcctt agatctctcg ccgccaatgc ttgttcaggc
     8341 acgccagaag gatgccgcag accattatct ggcgggagat atcgaatccc tgccgttagc
     8401 gactgcgacg ttcgatcttg catggagcaa tctcgcagtg cagtggtgcg gtaatttatc
     8461 cacggcactc cgcgagctgt atcgggtggt gcgccccaaa ggcgtggtcg cgtttaccac
     8521 gctggtgcag ggatcgttac ccgaactgca tcaggcgtgg caggcggtgg acgagcgtcc
     8581 gcatgctaat cgctttttac cgccagatga aatcgaacag tcgctgaacg gcgtgcatta
     8641 tcaacatcat attcagccca tcacgctgtg gtttgatgat gcgctcagtg ccatgcgttc
     8701 gctgaaaggc atcggtgcca cgcatcttca tgaagggcgc gacccgcgaa tattaacgcg
     8761 ttcgcagttg cagcgattgc aactggcctg gccgcaacag caggggcgat atcctctgac
     8821 gtatcatctt tttttgggag tgattgctcg tgagtaaacg ttattttgtc accggaacgg
     8881 ataccgaagt ggggaaaact gtcgccagtt gtgcactttt acaagccgca aaggcagcag
     8941 gctaccggac ggcaggttat aaaccggtcg cctctggcag cgaaaagacc ccggaaggtt
     9001 tacgcaatag cgacgcgctg gcgttacagc gcaacagcag cctgcagctg gattacgcaa
     9061 cagtaaatcc ttacaccttc gcagaaccca cctcaccgca catcatcagc gcgcaagagg
     9121 gcaggccgat agaatcatcg gtaatgagct ccggattacg cgcgcttgaa caacaggctg
     9181 actgggtgtt agtggaaggc gctggcggct ggtttacgcc cctttctgac actttcactt
     9241 ttgcggattg ggtaacacag gaacaactgc cggtaatact ggtggttggc gtgaaactcg
     9301 gctgtattaa tcacgcgatg ttgactgcac aggcaataca acacgccgga ctgacgctgg
     9361 cgggttgggt ggcgaatgat gtcacgcctc ctggaaaacg tcacgctgaa tatataacca
     9421 cgctcacccg catgattccc gcgccgctgc tgggagagat cccctggctt gcagaaaatc
     9481 cagaaaatgc ggcaaccgga aagtacataa accttgcctt gttgtagcca tcctgtattt
     9541 ggttaaattg cgagcgagat cgcgtcttcg attgactgca atttaaccaa ttaaattcta
     9601 aaataatcac gaaaaaaatt ttacttccgc ctcatgcggc gaatgtggga attgcccagg
     9661 cggcggggga taggggctgg agacagt
//
LOCUS       DAJSKJ010000147         9686 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000147 DAJSKJ010000000
VERSION     DAJSKJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.148"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="ON345_003066"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="ON345_003066"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR3073091.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVEAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLIDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="ON345_003067"
     CDS             834..1262
                     /locus_tag="ON345_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR3073092.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="ON345_003068"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="ON345_003068"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR3073093.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="ON345_003069"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="ON345_003069"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR3073094.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="ON345_003070"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="ON345_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR3073095.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNCESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="ON345_003071"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="ON345_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR3073096.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="ON345_003072"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="ON345_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR3073097.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="ON345_003073"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="ON345_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR3073098.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="ON345_003074"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="ON345_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR3073099.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9465)
                     /locus_tag="ON345_003075"
     CDS             complement(8281..9465)
                     /locus_tag="ON345_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073100.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
BASE COUNT         2456 a         2441 c         2568 g         2221 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccagggagga
       61 taacgttgcg tcagcaacgg cccgaagggc gaacgccagt gagtcatcct cccggatgca
      121 ccatcttctc ccaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatgtt gaggcaatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagcgt cgaaccgctt
      541 cctcttattg atgtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gctcacctgg gattacgccg ttattttgac
      661 gccgtcgtcg ctgccgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgttg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagtcat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctactggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag ctaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg acgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gttgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggcg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagaaaa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgacctg tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgaagcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacgcctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa attttaaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aatggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt cttattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga acgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgcctccgcc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcag ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag aatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttc ggtgcgggtt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa tatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagcgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa cgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcc
     5521 gtggtgatag tgaccggctg accgatacgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgccccag gctgtaccgc gcggcgggag acataaccgg tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgcga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgctttcgcg
     5941 agggcgattt tttgcacctc aatattcgcc tgcaactgtt tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagaacatcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatctgatt ccctgccacg
     6181 tatgcgtcat ctgtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccggtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggtttt ccagcagctt gctttgcatg
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tgggttatat aatcgccatc actttccagg cgagcccata ggccagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgcca cgccaaccag taacccaagc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcctggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gcagcttcaa cagcgggata accttgcagc aagccgctgc cggagaatgc
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgcg gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaatgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taaccagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctggtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcagggtgta ttcgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 tacggtgaga tccagcacca ggatgccgat aatcagcgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagcagaccg aaagttgtgg tgtggtgcga
     8641 tttaccctta tcggcaaaac cgcccgccgg acgagcgccc aacgctccgg cagctcccgc
     8701 aagtccaaac agaccaatga caccatcgct gtagttaaaa ggtggagcgg caagcaaaaa
     8761 ggccattgag gtccagagaa tgctgaagtt ggcaaaggtc aggcagccca gcaatgcgcg
     8821 ggtgcgcagg atcttatcgc tgataaacat gctgaaaacg gaacctaaca actgtgggta
     8881 gttgaggtgg gtttctgatt tcatttgtgg cagaccacgc cataatgcca gcgccatcag
     8941 tgccattaac accgaggcaa cccaaaagac ggtgcgccag ccgccgagat tcgccagcaa
     9001 tccggcaacg gtccgtgcga gtaagatccc caacagcaga ccgctcataa tagtgccaac
     9061 cactttgccg cgtttgtccg gtgaagccag cgtcgctgcc agcggaacca gaatttgtgc
     9121 cacgacagag aataaaccgg ttaatgccgt accgaggatc atcatcgcca gcgactgact
     9181 gctggcagtg attaacatgc cgcctgccgc cagtaaggtc atcgagacaa tcaggcggcg
     9241 gcgttcaaac atatcaccga ggggaacaag aaacagcaga cctgctgcat agcccaattg
     9301 cgcggcggta acaataaagc ctgccgaact ggcggaaagg gaaaagttac gcgcgatggt
     9361 gtcgagcaat ggctgagcgt aatagttact ggcgaccgcc agaccggtgg cgatagacat
     9421 cagcacgatc agcgccgggc taagctcatg attaggttta gtcattattt ttgttcatca
     9481 ggtgatgatt aagctggaaa tcaataatag cggatgttga tgaatatggg atgaaagata
     9541 gattgttgaa atgtgcgatg tggtttgtag gcatgataag acgcgccagc gtcgcatcag
     9601 gcattgtgca cagaacggcg gatgcggcga aaacgcctta tccgccatac ggaatgagtg
     9661 tattttgtag gcatgataag acgcgt
//
LOCUS       DAJSKJ010000148         9639 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000148 DAJSKJ010000000
VERSION     DAJSKJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.149"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            358..1221
                     /locus_tag="ON345_003076"
     CDS             358..1221
                     /locus_tag="ON345_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR3073101.1"
                     /translation="MNNSEGLKSFQQSLSGLPQWVTERILQQINQLTDYEPVIGIMGK
                     SGAGKSSLCNALFASEVSPVSDVAACTREPLRFRLQVGERYMTVVDLPGVGESGARDT
                     EYAALYREQLPRLDLVLWLIKADDRALAVDEHFYHQVIGEAYRHKVLFVISQSDKVEP
                     TSGGGPLSKEQKQNISRKICLLHELFQPVHPVCAVSVRLQWGLRVMAERMIKCLPREA
                     TSPVVSQLQPSFRTTVVREQARSDFGETVGAVLDSISAFPLIPAPVRAVIQAVRTTVV
                     SVARAVWDFFF"
     gene            1313..2134
                     /locus_tag="ON345_003077"
     CDS             1313..2134
                     /locus_tag="ON345_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR3073102.1"
                     /translation="MTRLASRFGAANLIRRDRPLTREELFRVVPSVFSEDKHESRSER
                     YTYIPTISLLDSLQREGFQPFFACQTRVRDPGRREHTKHMLRLRREGQITGKQVPEII
                     LLNSHDGTSSYQMLPGLFRAVCQNGLVCGESFGEVRVPHKGDVVSQVIEGAYEVLGIF
                     DRVEEKRDAMQSLLLPPPAQQALAKAALTYRFGEDHQPVTESQILSPRRWQDESNDLW
                     TTYQRIQENLIKGGLSSRNAKGGRSHTRAVRGIDGDVKLNRALWVMAEALLTQLQ"
     gene            2351..3052
                     /locus_tag="ON345_003078"
     CDS             2351..3052
                     /locus_tag="ON345_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR3073103.1"
                     /translation="MTQAERRHDRLAVRLSLIISRLVAGETLSVRKLAAEFGVSVRTL
                     RRDFRERLMYLDLEYQSGYCRLRTAGSETQMVPDVLIFAHRSGMAGLFPSFDRRLVNA
                     LLMCDESPCVIAPASPAPSPSGALSFWRLIQAITGRRRVTLIAEGQRCERLAPCRLLI
                     HQQAWYLVAEHDGHIAVFSLDEIHLVQPLQETFRRNDSLCRLVEDPGFIQSLPHFRFI
                     QQSLLTFVPADSPPE"
     gene            3093..3329
                     /locus_tag="ON345_003079"
     CDS             3093..3329
                     /locus_tag="ON345_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073104.1"
                     /translation="MPVIAIIAIVVIVIILNKTGVSDSLAALTLATVAALLTGGGAAG
                     AASVALTPFVGVPVGIFVGIYVFAKVVRLISGKK"
     gene            3329..3772
                     /locus_tag="ON345_003080"
     CDS             3329..3772
                     /locus_tag="ON345_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073105.1"
                     /translation="MKRKTLPLLALVATTLFLIACDDRSDDLKAISKFKDLTPPRFSD
                     VVSHQDDVSEEWSQVDYLSGPTLQVLRTRQSPDGCEDGSYYYLVDMQEKTVQPLMNAL
                     CIADNIKLEYQEVTDPYTKEKYFEYAHDGKLMGRLLIPSNPDNQE"
     gene            3795..4262
                     /locus_tag="ON345_003081"
     CDS             3795..4262
                     /locus_tag="ON345_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073106.1"
                     /translation="MTILSLSRFMLAGVLLASFNASAIPGFWQQGYGQGNTEYSVTEA
                     SGKTFTINCTGNPDQNGFYQHSVFLTLADDKMVSSHDDDTTITVVMDHQQYIIPSSLG
                     WRNGDNAWFDFISNISEAGQFDVYVNDHKAGTFTADRKNAEKVLSTPGDCSND"
     gene            4339..4578
                     /locus_tag="ON345_003082"
     CDS             4339..4578
                     /locus_tag="ON345_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCR3073107.1"
                     /translation="MTQSVLLPPGPFTRRQAQAVTTTYSNITLEDDQGSHFRLVVRDT
                     EGRMVWRAWNFEPDAGEGLNRYIRTSGIRTDTATR"
     gene            4676..5134
                     /locus_tag="ON345_003083"
     CDS             4676..5134
                     /locus_tag="ON345_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR3073108.1"
                     /translation="MITQTQHDLAPANQPEFELTVTPVPDEQRIDFWPQYFGAIPQWL
                     LLEPHIFAWMDRFCEGYSGGIWSFYTLSNGGAFMSPEPDNDETWRLFNCLNGNDAQMS
                     AEAAGIAVCLIAYSHHACRTECDAMTAHYYRLREYAMQHPEAHAILRIID"
     gene            5150..5626
                     /locus_tag="ON345_003084"
     CDS             5150..5626
                     /locus_tag="ON345_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR3073109.1"
                     /translation="MKQLSFLPGEMTPQDRRLIQRALRALDRYLHEPGVAFTSTHAVR
                     EWLRLHMAALEREEFRVLYLDNQNQLIAHETLFTGTINRTEVHPREVVKRALHFNAAA
                     VILAHNHPSGETTPSQADKTLTQRLVQVLQLVDIRVPDHLIVGGRQIYSFAEHGLL"
     gene            5635..5856
                     /locus_tag="ON345_003085"
     CDS             5635..5856
                     /locus_tag="ON345_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR3073110.1"
                     /translation="MKIISKRRAMTIYRQHPESRIFRYCTGKYQWHGSVCHYTGRDVP
                     DIAGVLAVYAERRQDRNGPYTCLMSITLN"
     gene            5875..6192
                     /gene="yafW"
                     /locus_tag="ON345_003086"
     CDS             5875..6192
                     /gene="yafW"
                     /locus_tag="ON345_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system antitoxin YafW"
                     /protein_id="HCR3073111.1"
                     /translation="MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIE
                     CRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYHNDLTCEADTLGSCGYVYIAIYP
                     TQR"
     gene            6213..6554
                     /gene="ykfI"
                     /locus_tag="ON345_003087"
     CDS             6213..6554
                     /gene="ykfI"
                     /locus_tag="ON345_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin YkfI"
                     /protein_id="HCR3073112.1"
                     /translation="MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFC
                     NEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGFSWQEQSPYLRAADILRARQATG
                     LLRQSRNNVVR"
     gene            complement(6936..7011)
                     /locus_tag="ON345_003088"
     tRNA            complement(6936..7011)
                     /locus_tag="ON345_003088"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6976..6978),aa:Thr,seq:cgt)
     gene            complement(7126..8379)
                     /gene="proA"
                     /locus_tag="ON345_003089"
     CDS             complement(7126..8379)
                     /gene="proA"
                     /locus_tag="ON345_003089"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR3073113.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNDATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(8391..9494)
                     /gene="proB"
                     /locus_tag="ON345_003090"
     CDS             complement(8391..9494)
                     /gene="proB"
                     /locus_tag="ON345_003090"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR3073114.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         2174 a         2682 c         2515 g         2268 t
ORIGIN      
        1 tgataatcag cattaatact tatgtaacag caagaattta tgtcttaaac gtagacagtc
       61 atcgtatggg ctgatgtttc tataccgtta aattgatgcc atccaactat aaaaccttgc
      121 tgcccaatgc cctatagctc cctcaggcaa tttgccctta tccactgttc tggtctgggg
      181 atgagggata tcagaataac ctaacgactc agggactggg atcacaggca accgtggctc
      241 gtcgcttacc ttaattcaaa ttattttcag atagatatca ttcttctgaa catacccgat
      301 taacggcaga atctcacgat tctgccgttt ttgtataatt tttcagagga gtagctaatg
      361 aacaattctg aaggtttgaa gtcgtttcag caatcgcttt ccggcctacc gcagtgggta
      421 acagagcgga ttctgcaaca aattaaccag ttaactgact acgagccagt gatcggcatt
      481 atgggtaaga gcggtgccgg taaatcctca ctctgcaatg ccctgtttgc cagtgaggta
      541 tcaccggtca gcgatgtggc cgcctgcacc cgtgaacctt tacgctttcg tctgcaggtt
      601 ggcgagcgct atatgaccgt cgtggattta cccggtgtag gcgaaagtgg cgctcgcgat
      661 accgagtatg ctgcgctgta tcgcgaacaa ctcccccggc tcgacctggt tctgtggctg
      721 attaaggccg atgaccgagc gctggcggtg gatgagcact tttatcatca ggtgattggg
      781 gaggcatacc ggcataaggt gctgtttgtt atcagccagt cggataaggt cgagcccacc
      841 agcggtgggg ggccattgtc caaagagcag aagcagaata tcagccgtaa aatctgtctg
      901 ctgcatgagc tgttccagcc cgtacatcca gtgtgtgccg tgtcggtccg tcttcagtgg
      961 ggactgcggg tgatggccga gcgaatgatt aagtgcctgc cacgtgaggc caccagcccg
     1021 gtggtgtcgc aactccagcc ttcctttcgc acgacggtag tccgggaaca ggcgcgtagc
     1081 gattttggtg aaaccgtcgg tgctgtactc gatagcatca gtgcctttcc cctgataccc
     1141 gccccggtgc gggccgtcat tcaggccgtg cgcaccaccg tggtgtcagt ggcccgtgcc
     1201 gtctgggatt tcttcttctg agtatttaac ccatccctgt tatttccctg accctgtcgc
     1261 cgatgcgacg gggatttctt ttatttgttt tcccattatg aggagtctgc ttatgacccg
     1321 tctggcttcg cgctttggcg cagcaaacct tatccgtcgc gaccgcccgt taacccgtga
     1381 ggagctgttt cgcgtggtgc ccagtgtgtt cagcgaggac aaacatgaat cccgcagtga
     1441 gcgttatacc tatataccta ctatctccct gctcgacagc ctacagcgag aaggcttcca
     1501 gccattcttt gcctgtcaga cccgcgtgcg tgaccccggt cgtcgtgaac atacaaagca
     1561 tatgctgcgt ctgcggcgtg aggggcagat taccggtaaa caggtaccag aaattatcct
     1621 gctcaactcc cacgatggca ccagttcgta tcagatgttg ccgggactat ttcgtgcggt
     1681 ttgtcagaac gggctcgtct gcggtgagtc gtttggcgag gtgcgggtgc cacacaaggg
     1741 ggacgtggtg agtcaggtga ttgaaggcgc gtatgaggtg ctggggattt ttgaccgggt
     1801 ggaggagaaa cgggatgcca tgcagtcgtt gctgttgcca cccccggcac agcaggcact
     1861 ggcaaaagcc gccctcacat accgctttgg tgaagaccac cagccggtga ctgaatcgca
     1921 gatcctctcc cctcgccgct ggcaggatga gagcaatgac ctgtggacca cgtaccagcg
     1981 tattcaggag aacctgatta agggcgggct cagtagccgt aatgccaaag gaggacggtc
     2041 acatacccgt gccgttcgcg gtatcgacgg ggacgtgaaa cttaaccggg cactgtgggt
     2101 gatggcggaa gccctgctca cgcaactgca gtgaccgttt aatgttgctg ctttgttaat
     2161 atcggacacc acctgtccgc atcgcaccgt gctgacggtc ctcactcacc gagggaaggc
     2221 ctgtaacccc cgctgcctct ggcttttgca gaaatgaaaa atagtttctg tggtgtccat
     2281 accctgtccg accccctctt taaagtaatc acatcatttt cagtcagtta actttcacgg
     2341 agaacgtctc atgacacagg cagaacgccg ccatgaccgg ctggctgtca ggctgtcact
     2401 gataatcagc cgtctggtgg caggggaaac gctgagtgtg cgtaagcttg ccgctgagtt
     2461 tggtgtgtcg gtgcgcacgc tgcggcgtga ttttcgtgag cggctgatgt atctggacct
     2521 ggagtatcag tccggatatt gccgcttacg cactgcgggc agtgagacgc agatggtgcc
     2581 cgacgtgctt atctttgccc accgcagcgg gatggccggt cttttcccta gctttgaccg
     2641 tcgtctggta aacgcactgc tgatgtgcga tgagtctccc tgcgtaatcg cacccgccag
     2701 tccggctcct tcgccatcag gcgcattgtc tttctggcga ctgattcagg ccattaccgg
     2761 gcgcaggcgg gtgactctga ttgccgaggg gcagcgctgt gagcggctgg ctccctgccg
     2821 gttactcatc caccagcagg cctggtatct ggtggcagag cacgacggac atatcgccgt
     2881 attctccctg gatgagatcc atctggttca gcccctgcag gagactttcc ggcgtaacga
     2941 cagtctgtgt cgtcttgttg aagacccggg ctttattcag tccttacccc attttcgctt
     3001 tatccagcag tcactgctta cgtttgttcc ggccgacagc ccaccagaat agcgtaagcg
     3061 ttgttatcaa cccggcaaca gggaggagcc ctatgcccgt tattgccatt atcgccattg
     3121 ttgtcatcgt catcattctg aacaaaaccg gggtgtccga cagtctcgcg gccctgaccc
     3181 ttgccactgt tgccgcactg ctgacgggag gtggcgcagc cggtgctgcc agtgtcgcgc
     3241 tgacaccgtt cgtcggcgtg ccggtgggta ttttcgtggg catttatgtc tttgccaaag
     3301 tggttcgtct gatttcagga aaaaaataat gaaacgtaaa acactgcctc tgctggcgct
     3361 ggttgccacc actctgtttc tcatcgcatg cgatgacaga agtgatgacc tgaaggccat
     3421 cagtaaattt aaggacctca ccccaccgcg tttcagtgat gtggtcagcc accaggatga
     3481 tgtcagcgaa gaatggtcac aggttgacta cttatccggt cccaccttgc aggttttacg
     3541 tacccgccag tcgcccgatg gctgcgagga tggcagttac tactacctcg tggatatgca
     3601 ggaaaaaacc gtccagccac tgatgaatgc gctgtgtatt gccgataaca tcaaactgga
     3661 ataccaggag gtgacggacc cgtataccaa agaaaaatac tttgagtacg cccatgacgg
     3721 caaactgatg gggcgactgc tgataccctc aaaccctgac aatcaggaat aaaacaacga
     3781 caaaggagac aagaatgaca atactttcac taagtcggtt tatgctggcg ggtgtactgc
     3841 tcgcgagctt taatgcctct gctatcccgg ggttctggca gcagggttac ggtcagggca
     3901 atacggaata cagcgtgacc gaagccagcg gaaagacgtt taccatcaac tgcacaggga
     3961 acccggacca gaatggtttc tatcagcatt cagtctttct tacccttgcc gatgacaaga
     4021 tggtcagttc gcacgatgac gacactacta tcaccgtagt gatggatcac cagcagtaca
     4081 ttattccgtc cagcctgggc tggcgtaacg gcgataacgc ctggtttgac ttcatcagca
     4141 atatctctga ggccgggcag ttcgacgtct acgtcaatga ccacaaagca gggaccttca
     4201 ctgcggaccg gaagaacgct gagaaagttc tgtccactcc cggagactgc agcaacgact
     4261 gatagtagca tcttccccag caaatccacc ccgacagcta gcaggctgcc ggggttttct
     4321 tttatcagga gcccgaaaat gacccaatcc gtgttgctgc caccggggcc tttcacccgg
     4381 agacaagcgc aagcggtcac taccacgtac agcaatatca cactcgaaga cgaccagggc
     4441 agtcacttcc gtctggtggt tcgtgatact gaaggccgga tggtctggcg ggcatggaac
     4501 tttgagccgg atgccggtga aggtcttaac cgctatatcc gcacctcagg catccgtaca
     4561 gacacggcca cccgctgatc gcgaagcatt tacccgcatt cacctccccg aacacacttt
     4621 atatccccat acgccagcca tcgccgctgg cgtttttatt gacggagaca tacccatgat
     4681 aacacagacg cagcacgacc tcgcacccgc taaccaaccc gaatttgaac tgaccgtcac
     4741 gccggtcccc gatgaacagc gtatcgattt ctggccacag tactttggcg ctatcccgca
     4801 gtggctactc ctggagccgc atatcttcgc ctggatggac cgcttctgtg agggctacag
     4861 cggtggtatc tggtcgttct acaccctcag caatggcggc gcatttatgt cccccgagcc
     4921 tgacaacgat gagacatggc gtctgtttaa ctgcctgaac ggtaacgatg cccaaatgag
     4981 tgcagaagca gcaggtattg ctgtctgcct gattgcgtat agccaccatg cctgccgtac
     5041 agaatgtgac gcgatgaccg cacactatta ccgcctgcgg gagtatgcca tgcaacatcc
     5101 agaggctcac gccattctgc gtattatcga ctgaccgaag gagcaacaga tgaaacagct
     5161 ttccttttta cccggcgaga tgacgccaca ggaccggcgt ctcattcagc gggcgctcag
     5221 ggctctggac cgctacctgc atgagcccgg cgtagccttc acctctaccc acgccgtacg
     5281 tgaatggctg cgactgcata tggccgcgct tgagcgggaa gagttccggg tgttgtatct
     5341 ggacaaccag aatcagttga ttgcccatga aacgctcttc accggcacga ttaaccgcac
     5401 cgaggtgcat ccccgggagg tggtcaaacg tgctctgcac ttcaacgcgg cggcggtgat
     5461 actcgcgcat aaccatcctt ccggcgagac gacacctagc caggccgaca aaaccctcac
     5521 gcagcgactg gttcaggtgc ttcagctggt ggatatccgt gtccctgacc atctgattgt
     5581 cggtggcagg caaatctatt cgttcgcaga acacggtctg ctttgaggta ttacatgaaa
     5641 attatcagta aacgcagggc aatgacgata taccgccagc atcctgagtc ccgaatcttt
     5701 cgctactgca ccggcaaata ccagtggcac ggtagcgtct gtcattacac cggcagggac
     5761 gttccggata tcgccggagt cctcgcggta tacgccgaac gccgccagga ccgcaatggg
     5821 ccctatacct gcctgatgag catcaccctg aactgacaat agtggagaac tgtaatgagc
     5881 aaccctacca ggggcctgca gcgggagatt acactgcgcc tgggagcccg tctggtgcag
     5941 gaaggcaacc gactgcatta tctggctgac cgggccagca tcaccggcaa gttcagtgac
     6001 atcgaatgcc ggaagctgga tgaaacattc ccgcacttta tccgccagat ggaatcgatg
     6061 ctgaccaccg gtgaactcag cccccaccat gcccactgcg ttaccctgta ccacaacgat
     6121 ttaacctgcg aagccgacac ccttggcagt tgcggctacg tatacatcgc catttacccc
     6181 actcagcgtt aattacctac acgagagcaa acatgaaaac tttacctgca acaactcagc
     6241 gggcggtgaa gccctgcctg tcacccgtgg ctgtctggca aatgttactg acacgtctgc
     6301 tggaacagca ctatggtctg acaataaacg acacgccatt ctgcaatgag gctgtgatta
     6361 aggaacacat cgatgccggt atcaccctag ccgatgccgt gaattttctg gtagaaaaat
     6421 acgagctggt tcgtatcgac aggaagggat ttagctggca ggaacaatct ccttatctcc
     6481 gggctgcaga cattctgcga gcgcggcagg caactggctt gttgcggcaa agccgtaaca
     6541 acgtagtacg atgaacattg cgtacaacct tcccgattta catttctgaa cttcctccct
     6601 tgtttaccta ttgcgtaatg cgcctgctac tacccggcag gcgcgttatc tttttacgga
     6661 caaacaatca tgcaaccaga agttgaagta ttaaccgatc ataatgagct aatttgttcg
     6721 agctttattg aacacattgc caacacatta aatttaggag tggtgtacaa ctaaacctgc
     6781 agccaaggat gtatagtgag cgaagcccta tcaggccttt ttggtcagta gataagattg
     6841 atcttcgttg atagaattta cttacaccag ctgttacatt aagataattt tttggtggga
     6901 gaatgataag atcttacgta acaatttgat tttaatggtg ccgataatag gagtcgaacc
     6961 tacgaccttc gcattacgaa tgcgctgctc taccaactga gctatatcgg ccctgaaagg
     7021 acatgttcac gaacgtgaat cacggtggac aaggttaaaa ctaaccgggc gatgcgtcaa
     7081 tggccttgtg aatcaaatgg ctacttttgc atcacccggt tttatttacg cacgaatggt
     7141 gtaatcacca atgccgatcc acttgtaagt ggtcagtgct tccagcccca ttgggccacg
     7201 cgcgtggagt ttttgtgtgc ttaccgccac ttccgcaccc agaccaaact ggccgccgtc
     7261 ggtaaaacgc gtagaggcgt taacgtaaac agcggacgaa tccacttcgt taacaaaacg
     7321 ctgggcgttg cgcatatcgc gggtcaggat cgcatcggag tgttgtgtgc cgtgttcacg
     7381 aatatgggcg atggcatcgt caagatcgct gacgattttg acgttcaaat ctaatgacag
     7441 aaactcatcg tcatactctt cggctttaac agcaaccacc ttcgcagggc ctgcctgcaa
     7501 ctgcgccagt gcagctgcat ctgcgtgtaa tgtcacgccg ctttccgcca tttgtttgct
     7561 taatgcgggc aggaagctat cggcgatgtt tttattcacc agcaacgttt caaccgtatt
     7621 acatgtgctc ggacgctgag ttttcgcgtt gacgattact tttaatgctt cagcgatctc
     7681 tacactttca tcaacgtaaa tatggcatac gcctatacca cctgtgatca ccgggattgt
     7741 cgactgttca cggcacagtt tatgcaaacc agcgccacca cgcgggatca gcatgtcgat
     7801 gtatttatcc atacgcagca tttcactgac cagcgcacgg tcaggattat caatcgcctg
     7861 cacggcaccc gccggtaagc cgcaggattt cagggcgtcc tgaatcaccg ccaccgttgc
     7921 atcgttagtg cgacacgttt ctttgccacc gcgcaggatc actgcgttac cggttttcag
     7981 gcacagcgaa gcgacatcaa ccgtcacgtt cgggcgcgct tcataaatca cgccaataac
     8041 ccccagcggt acgcgacgac gctcaagacg caggccgctg tccagtacgc cgccatcgat
     8101 tacctgcccc accggatcgg cgaggttgca cacctgacgt acatcgtcgg caatgccttt
     8161 cagccgtgcg ggcgtcagtg ccagacggtc aagcatcgct tcgctaaggc cattggctcg
     8221 cgcgtcagca acatcctggg cgttagcgtt gaggatgatt tcgctttgtg cttccagttc
     8281 atcggcgatt ttttccagca cgcgattttt ttcgcggctg gagagttgcg ctaatttata
     8341 cgaggcttgc ttcgcggcaa tgcccatttg ttccagcatc agcctgctcc ttaacgggta
     8401 atcatgtcat cacggtgaac ggcaaccggg ccgtattcat atcccagtat tgcatcaatt
     8461 tcttgcgagt ggtgcccggc aatacggcgt aatgcatcgc tgttgtaacg actgacgccg
     8521 tgggcgatat cgcgaccttc gaggttgcaa atgcggatga cttcaccacg cgagaaattg
     8581 ccagtcacgc ttttaatgcc tttcggcaac agggagctgc cgcgttcaag aatggcggca
     8641 gttgcccctt catctaccgt gatttcaccc gccggcggcg caccgaaaat ccagcgttta
     8701 cggttttcaa gcggagtcgc ctgggcatgg aacagcgtac cgacggaaat gccttccatc
     8761 acatcaccaa taacgcccgg cttgctgccc gcggcaataa tggtgtcgat acccgcacgg
     8821 caagccacgt cagcggcctg caatttggta ctcatgccgc cagttccgag gcctgaaacg
     8881 ctgtcaccgg caatcgcgcg cagtgcgtca tcaatgccgt aaacatcttt aatcagttct
     8941 gcctgcggat tgctgcgcgg gtcagcggta tacaaacctt tttgatcggt cagcagcaac
     9001 agtttatcgg cacccgccag aatcgccgcc agcgcagaaa ggttatcgtt atcgccgact
     9061 ttaatctctg ccgtagcgac agcatcgttc tcattgatta ccggaacgat attgttatcg
     9121 agcaacgcac gcagggtgtc gcgggcgttc aggaagcgtt cacggtcttc catatcagca
     9181 cgggtcagca gcatttgccc gacgtgaatg ccataaatcg aaaacagctg ttcccacagt
     9241 tgaatcagtc gactctgccc taccgctgcc agcagttgtt tcgaggcgat agtcgctggc
     9301 agttccgggt aacccaggtg ctcacgtccg gcggcgatcg cgcccgacgt cacaataaca
     9361 atccgatgcc cggcggcatg taactgcgcg cactggcgaa caagttcaac gatatgggca
     9421 cggttcagac ggcgcgatcc gcctgttagc acactggtgc cgagttttac caccagcgtc
     9481 tggctgtcac tcatgattct ctgccattca attttaggaa aaatgatatc aaacgaacgt
     9541 tttagcagga ctgtcgtcgg ttgccaaccg tctgcgagca aagcatggcg ttttgttgcg
     9601 cgggatcagc aagcctagcg gcagttgttt acgctttta
//
LOCUS       DAJSKJ010000149         9572 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000149 DAJSKJ010000000
VERSION     DAJSKJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.150"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(44..1024)
                     /gene="ygfZ"
                     /locus_tag="ON345_003091"
     CDS             complement(44..1024)
                     /gene="ygfZ"
                     /locus_tag="ON345_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR3073115.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1267..1533
                     /gene="sdhE"
                     /locus_tag="ON345_003092"
     CDS             1267..1533
                     /gene="sdhE"
                     /locus_tag="ON345_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR3073116.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            1514..1927
                     /locus_tag="ON345_003093"
     CDS             1514..1927
                     /locus_tag="ON345_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR3073117.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR"
     gene            complement(1961..2482)
                     /gene="fldB"
                     /locus_tag="ON345_003094"
     CDS             complement(1961..2482)
                     /gene="fldB"
                     /locus_tag="ON345_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR3073118.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2594..3490
                     /gene="xerD"
                     /locus_tag="ON345_003095"
     CDS             2594..3490
                     /gene="xerD"
                     /locus_tag="ON345_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR3073119.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQTPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3514..4224
                     /gene="dsbC"
                     /locus_tag="ON345_003096"
     CDS             3514..4224
                     /gene="dsbC"
                     /locus_tag="ON345_003096"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR3073120.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            4230..5963
                     /gene="recJ"
                     /locus_tag="ON345_003097"
     CDS             4230..5963
                     /gene="recJ"
                     /locus_tag="ON345_003097"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR3073121.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLGPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6054..7152
                     /gene="prfB"
                     /locus_tag="ON345_003098"
     CDS             join(6054..6128,6130..7152)
                     /gene="prfB"
                     /locus_tag="ON345_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR3073122.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7162..8679
                     /gene="lysS"
                     /locus_tag="ON345_003099"
     CDS             7162..8679
                     /gene="lysS"
                     /locus_tag="ON345_003099"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3073123.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8722..9270)
                     /gene="idi"
                     /locus_tag="ON345_003100"
     CDS             complement(8722..9270)
                     /gene="idi"
                     /locus_tag="ON345_003100"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR3073124.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            9325..9396
                     /locus_tag="ON345_003101"
     CDS             9325..9396
                     /locus_tag="ON345_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073125.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(9393..9518)
                     /locus_tag="ON345_003102"
     CDS             complement(9393..9518)
                     /locus_tag="ON345_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073126.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
BASE COUNT         2342 a         2391 c         2587 g         2252 t
ORIGIN      
        1 cagcgtcgca tcaggcgata aatgcctgat gcgataaaga gatttactct tcgagcgaat
       61 acggcagcgg ctcgatatgc aatgtattcg catcgtcgcg tacgcggaag atgctatccg
      121 gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg
      181 cagccagcac cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac
      241 cagcttccgg cagtcggctg gcgctacctg ccagcaacca gagcgcacgt ttattggcac
      301 cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga
      361 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat
      421 caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
      481 ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg gttacgatca
      541 ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct ttgactacct
      601 gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac
      661 cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg
      721 catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccatgcaa
      781 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat
      841 gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc acctgaccct
      901 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca
      961 tcagcgtcag cggtaaacgg gcagaagccg taggctgacg gggaggaaaa ggtgtaaaag
     1021 ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg
     1081 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac ttccaaaaaa
     1141 atgaagccat tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt actgatattc
     1201 ccgtaaaaga aggcgaaaaa cacgttacaa tagccgggta ctggattttc gtgagacagg
     1261 aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg tggtatgcgc
     1321 gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt aagcgatgac
     1381 gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt taactggctg
     1441 atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga tggtccgact catccagaca
     1501 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg
     1561 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccact
     1621 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt gcgttcgcag
     1681 ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg
     1741 ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat
     1801 gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat
     1861 ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagatc
     1921 aagatgacgg caaagcatat gcgctaagcc gtcagtaaca tcaggcgtaa tgctctgcca
     1981 tttcgttgag gatttgttca caccagctct gaatacgctc gtcgctaagg tcatactggt
     2041 tagtttcgtc cagcgccagc cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc
     2101 tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga
     2161 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
     2221 caagcccata cagcgcaaca attttacctt cgaggttcag gtcgtcgagc tgatcccaga
     2281 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
     2341 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
     2401 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
     2461 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcggct
     2521 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
     2581 ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg atgctctgtg
     2641 gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat
     2701 ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca
     2761 ggcattactg gcagaaaggc tggagggcgg gtataaagcc accagctcgg cacgtttgct
     2821 gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
     2881 cagtgcgcat ctcgcttcac cgaaattgcc acagcgtttg ccaaaagatt taagcgaagc
     2941 gcaggtcgaa cgtttattac agacaccatt aattgatcag ccactggagc tacgcgataa
     3001 agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcggactgac
     3061 aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga
     3121 gcgcctggtg ccgttaggtg aagaggcggt ttactggctg gaaacgtatc tcgagcatgg
     3181 gcgaccgtgg ttgttgaacg gtgtctctat tgatgtgttg ttccccagcc agcgtgcgca
     3241 gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc tggcgggtat
     3301 cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa
     3361 tcatggtgcg gatttacgtg tggtgcagat gttgttgggt catagcgatc tatccaccac
     3421 ccaaatttat acgcatgtcg ccaccgagcg tttgcgtcaa cttcatcaac agcatcaccc
     3481 gcgggcttga ttgctgagaa aacaggacga tttatgaaga aaggttttat gttatttacg
     3541 ttgttggcgg cgttttcagg ctttgttcag gcggatgacg cggcaattca acaaacgtta
     3601 gccaaaatgg gcattaaaag cagcgatatt cagccagcac ccgtggctgg catgaagact
     3661 gtgctgacta acagcggtgt gttgtacatc accgatgacg gtaaacatat cattcagggg
     3721 ccaatgtatg acgttagcgg cacggctccg gtcaatgtca ccaataagat gctgttaaag
     3781 cagttgaatg cgcttgaaaa agagatgatc gtttataaag caccgcagga aaaacacgtc
     3841 atcaccgtgt ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca
     3901 gactataacg cgctggggat caccgtgcgt tatcttgcct tcccgcgcca ggggctggac
     3961 agcgatgcag agaaagaaat gaaagctatc tggtgtgcga aagataaaaa caaagcgttt
     4021 gatgatgtga tggcaggtaa aagcgtcgca ccagccagtt gcgacgtgga tattgccgac
     4081 cattacgcac ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat
     4141 ggcacacttg tcccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac
     4201 caaaaaatga ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg
     4261 aagtcgatga aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg
     4321 ccagccgggg agtacgcagt gcgcaagaac tggaacgcag tgttaaaggt atgctgccct
     4381 ggcagcaact gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag
     4441 gaacgcggat tattgtggtc ggtgattttg atgccgacgg cgcgaccagc acggctctaa
     4501 gcgtgctggc gatgcgctcg cttggttgca gcaatatcga ctatctggta ccaaaccgtt
     4561 tcgaagacgg ttacggctta agcccggaag tagtcgatca ggcccatgcc cgtggcgcgc
     4621 agttaattgt cacggtggat aacggtattt cctcccatgc gggcgttgaa cacgctcgct
     4681 cgttgggcat tccggttatt gttaccgatc accatttgcc gggcgaaaca ttacccgcag
     4741 cggaagcgat cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag
     4801 gcgtgggtgt ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct
     4861 ggtttgatga gcgtggcatc gcaattccta acctggcaga actgctggat ctggtcgcgc
     4921 tgggaacagt ggcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg
     4981 ggatgagtcg catccgtgcc ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg
     5041 caaaccgtga tgcacaaaaa ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc
     5101 tcaatgctgc cggacgactg gacgatatgt ccgtcggtgt ggcgctcttg ctgtgcgaca
     5161 acatcggcga agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag
     5221 agatcgaaca aggaatgcaa gttgaagccc tgaccctgtg cgagaaactg gagcgaagtc
     5281 gcgacacgct acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg
     5341 gtattctggc ttcgcgcatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag
     5401 caggtgatgg tacgctgaaa ggttcaggtc gctccattca ggggctgcat atgcgtgatg
     5461 cactggagcg attagacaca ctctaccctg gcatgatact gaagtttggc ggtcatgcga
     5521 tggcggcggg tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg
     5581 aactggttac agagtggctg ggcccttcgc tattgcaagg cgaagtggtg tcagacggtc
     5641 cgttaagccc ggccgaaatg accatggaag tggcgcagtt gctgcgcgat gctggcccgt
     5701 gggggcagat gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc
     5761 tggtgggcga acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg
     5821 atggtattgc ttttaatgtc gataccgccc tctggccgga taacggcgtg cgcgaagtgc
     5881 aactggctta caagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca
     5941 tcgacaatat ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta
     6001 caatcccgct cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg
     6061 aaattaatcc ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg
     6121 ggtatctttg actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa
     6181 cagccggatg tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc
     6241 ctcgaagccg ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt
     6301 ctgctggaac tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa
     6361 ctcgacgccc tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa
     6421 tatgacagcg ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag
     6481 gactgggcga gcatgcttga gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa
     6541 actgaaatca tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa
     6601 atctccggcg attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg
     6661 cgtaaaagcc cgtttgactc cggcggtcgt cgccacacat cgttcagctc tgcgtttgtt
     6721 tacccggaag ttgatgatga tattgatatc gaaatcaacc cggcggatct gcgcattgac
     6781 gtttatcgcg cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt
     6841 attacccaca tcccaaccgg gatcgtgacc caatgccaga acgaccgttc ccagcacaag
     6901 aacaaagacc aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag
     6961 aaaaatgccg agaaacaggc gatggaagat aacaaatccg acatcggctg gggcagccag
     7021 attcgttctt atgtccttga tgactcccgc attaaagatc tgcgtaccgg ggtagaaacc
     7081 cgcaacacgc aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa
     7141 gcagggttat gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc
     7201 gatcttaaca atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg cgagcagggg
     7261 attgccttcc cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc
     7321 gacggtaaag agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc
     7381 atgatgaccc gccgtattat gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc
     7441 cgcattcagc tgtacgttgc ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc
     7501 aaaaaatggg acctcggtga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc
     7561 ggcgaactgt ctatccactg taccgagctg cgtctgctga ccaaagcact gcgtccgctg
     7621 ccggataaat tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatctggat
     7681 ctcatctcta acgatgaatc ccgtaacacc tttaaagtgc gctcgcagat cctctctggt
     7741 attcgccagt tcatggtgaa ccgcggcttt atggaagttg aaacgccgat gatgcaggtg
     7801 atccctggcg gtgccgctgc gcgtccgttt atcacccacc ataacgcgct ggatctcgac
     7861 atgtacctgc gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag
     7921 cgtgtattcg aaatcaaccg taacttccgt aacgaaggta tctccgtacg tcataaccca
     7981 gagttcacca tgatggaact ctacatggct tacgcagatt acaaagatct gatcgagctg
     8041 accgaatcgc tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac
     8101 ggcgacgtga cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc
     8161 aagaaatatc gcccggaaac cgacatggcg gatctggata acttcgactc tgcgaaagcg
     8221 attgctgaat ctatcggtat ccacgttgag aagagctggg gtctgggtcg tatcgttacc
     8281 gagatcttcg aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat
     8341 ccggcagaag tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc
     8401 tttgagttct tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg
     8461 gaagatcagg cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa
     8521 gcgatgttct acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca
     8581 ggtctgggga ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc
     8641 gacgttattc tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac
     8701 cccggcaaat gtcggggttt tttaattgtg ctgcgcgaaa gtagacaaca attttcttgc
     8761 ttcactattg gctgcctgca tcaccatcca cggactgaac gcccacggtg tggcatcaat
     8821 accgtgtaat acatctgcta aatcacacca ttgataatcc atcacttcat catcgttgat
     8881 ctgtaacgca ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc
     8941 attcggatcg gtggcgcgat agcgaaagtc aggatagata gattcaggag gcgtaatttc
     9001 cacgccaagc tcataacggc aacggcggat caccgcgtct tcgttgcttt ctcccagttg
     9061 tgggtgccca caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg
     9121 gcgggtaact aataattgtc ctttggcatt aaacagccaa ctggagaacg cgagatgtaa
     9181 gcgggtgtct gccgtgtgtg cggcatactt ttccagcgta cccgtgggaa ctccctgtgc
     9241 attcaataaa atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat
     9301 cattatagat aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc
     9361 gttttgagcg gatgccgaca ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag
     9421 cgactttcaa tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc
     9481 aacagcagac tgaatatagc gcgcattaaa aaattcatat tattctccat gctcattttt
     9541 cttcagcttg cgcatcagaa gccacatacg ta
//
LOCUS       DAJSKJ010000150         9515 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000150 DAJSKJ010000000
VERSION     DAJSKJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.151"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..70
                     /gene="rpsV"
                     /locus_tag="ON345_003103"
                     /gene_synonym="sra"
     CDS             <1..70
                     /gene="rpsV"
                     /locus_tag="ON345_003103"
                     /gene_synonym="sra"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="protein D; stationary-phase-induced
                     ribosome-associated; in Escherichia coli this protein
                     becomes associated with the ribosome; expression increases
                     during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal subunit S22"
                     /protein_id="HCR3073127.1"
                     /translation="VTEGDKSSVVNNPTGRKRPAEK"
     gene            227..1924
                     /gene="maeA"
                     /locus_tag="ON345_003104"
     CDS             227..1924
                     /gene="maeA"
                     /locus_tag="ON345_003104"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR3073128.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCSRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQNVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2058..3068
                     /gene="adhP"
                     /locus_tag="ON345_003105"
     CDS             2058..3068
                     /gene="adhP"
                     /locus_tag="ON345_003105"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR3073129.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3214..3498
                     /locus_tag="ON345_003106"
     CDS             3214..3498
                     /locus_tag="ON345_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR3073130.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLSG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(3727..4380)
                     /gene="fdnI"
                     /locus_tag="ON345_003107"
     CDS             complement(3727..4380)
                     /gene="fdnI"
                     /locus_tag="ON345_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR3073131.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFMRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4373..5257)
                     /gene="fdnH"
                     /locus_tag="ON345_003108"
     CDS             complement(4373..5257)
                     /gene="fdnH"
                     /locus_tag="ON345_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR3073132.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELADQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5270..8317)
                     /gene="fdnG"
                     /locus_tag="ON345_003109"
     CDS             complement(5270..8317)
                     /gene="fdnG"
                     /locus_tag="ON345_003109"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7730..7732),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR3073133.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYE
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFSNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8549..9430
                     /gene="yddG"
                     /locus_tag="ON345_003110"
     CDS             8549..9430
                     /gene="yddG"
                     /locus_tag="ON345_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR3073134.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2272 a         2490 c         2438 g         2315 t
ORIGIN      
        1 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
       61 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      121 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      181 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      241 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      301 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      361 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      421 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acactaacga
      481 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      541 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      601 gtttatctct taccagaacc ggcacaatat ggacgatatt ctgcaaaacg tgccgaacca
      661 taatattaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
      721 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
      781 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacagctgct
      841 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
      901 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccggacgtgc tgttgcagtt
      961 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1021 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1081 cagccgcgcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1141 agcgggatgc ggcattgccg aaatgatcat ctcccagacc cagcgcgaag gattaagcga
     1201 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcttgctga ccgacaagat
     1261 gccgaacctg ctgcctttcc agaccaaact ggtgcagaag cgcgaaaacc tcagtgactg
     1321 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1381 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1441 taaacactgt tcgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1501 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca ctggcagccc
     1561 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1621 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     1681 gatgctgatg tcggcaagtg aaacgcttgc tcagtattcg ccgctggtcc tgaacggcga
     1741 aggtctggta ctaccggaac tgaaagatat tcagaatgtc tcccgcgcaa ttgcgtttgc
     1801 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acgtctgctg aagctttgca
     1861 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     1921 ctaagcctgc gcccggtagt gaaggctacc gggctatttc cctctccctt tttcagatct
     1981 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2041 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2101 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2161 gtgtatgtca taccgatctt catgtcaaga atggcgattt tggtgacaaa actggggtaa
     2221 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2281 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2341 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2401 gcgggatggc ggaagagtgc atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc
     2461 tggactcggc ggcggccagc agcattacct gtgcgggggt caccacctac aaagccgtta
     2521 aactgtcaaa aattcgtcca gggcagtgga ttgctatcta cggtcttggc ggtctgggta
     2581 acctcgctct gcaatacgcg aagaatgtct ttaacgcgaa agtgatcgcc attgatgtca
     2641 atgatgagca gttaaaactg gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca
     2701 ccgaagacgc cgccaaaatt gtgcaggaaa aaaccggtgg tgcacacgcg gcggtggtaa
     2761 cagcagtagc taaagctgcg tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg
     2821 ttgtggctgt cggtctgccg ccggagtcta tgagcctgga tatcccacgt cttgtgctgg
     2881 atggtattga agtggtcggt tcgctggtcg gcacgcgcca ggatttaact gaagccttcc
     2941 agtttgccgc cgaaggtaaa gtggtgccga aagtcgccct gcgtccgtta gcggacatca
     3001 acaccatctt tactgagatg gaagaaggca aaatccgtgg ccgcatggtg attgatttcc
     3061 gtcactaaga ggcctttgct gcgactgcca tgttcgggtc gcagcatcgc acactctcca
     3121 acatgaaatg gctaaatgga ttattgttgc gtaggaagga acaataatga tagtgttccc
     3181 agtgaaggaa cgctactgag agtaaagaca ctaatgaaaa tggccaatca tccccgcccg
     3241 ggggacatta ttcaggaatc actggacgaa cttaatgtca gcctgcgcga gtttgccaga
     3301 gcaatggaaa ttgcgccctc aacggcaagc cgattgctga gcggaaaagc agctttgacg
     3361 ccagaaatgg caattaaact ttccgtggtg atcggcagtt cgccgcaaat gtggctgaat
     3421 ctgcaaaacg cctggagtct ggcagaggct gaaaaaacgg tggatgtgtc gcgactacgc
     3481 cgtctggtta cgcaataaaa aaatggcgcc ttataaagcg ccatttgaaa ctgatgacaa
     3541 acgcaacatt gcctgatgcg ctacgcttat caggcctaca cgttcactgc aatctattgg
     3601 atttgaaggg tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaagcct
     3661 acgttgtcta caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa
     3721 aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata
     3781 ccagcgcgga tggtgtttct tcgcccagcg acggcttacc ttcccttcga tcatcccttt
     3841 aatcgatcct ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat
     3901 gatgccggca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg
     3961 cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat
     4021 gatcgaccag aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg
     4081 ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg
     4141 gatgttgtga tgcacaaaac gcataaacat aaacatcagc gcgacgaaaa tcgcaatgcc
     4201 gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg
     4261 cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac
     4321 ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat
     4381 gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgata tagtggaaaa
     4441 tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc
     4501 acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac agctccggct
     4561 gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc gggttgtaga
     4621 cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctggtcc gccagctcca
     4681 gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg
     4741 gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct
     4801 ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc
     4861 agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg
     4921 ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta cggatcagcc
     4981 actccagctt gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc
     5041 tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt
     5101 tccactccga acacgccacc tgacaggctt tacaaccgat acaggtggaa acgtcgataa
     5161 gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg
     5221 cggacctttt gataatgtcc tgcgtttcca tagccatctg ttcgccccct tatgccttct
     5281 cgatgtttac taagaacgct ttatattccg gcgtttgaga gtttgcatca ccgacattcg
     5341 gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggatcg
     5401 gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta
     5461 ccacagccac cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat
     5521 taatgccttt cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcta
     5581 ttgcgttgag caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg
     5641 tacccacata cgggaactgc tcttttttac ccatccgcaa cgcgtcttgt tcatacagac
     5701 gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa
     5761 tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc
     5821 ccatcccttc cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgctga
     5881 agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg
     5941 gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg cgacggttga
     6001 gcggccacgc ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc
     6061 ggttagccat ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg
     6121 cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa
     6181 tcagcacgcc attagcgtca tagagatctt ccagcgcata gccgttgttc tctttagcca
     6241 cttcgtcaga ttgcggttcg tgcggttgct tgtagttcca gctcatcttc atcagcggtt
     6301 ctacgccttt accaccttcg gcctggtaca gctcgcgcag atgatggtaa atacccgcca
     6361 gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca
     6421 accagcgacc ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaagggagac
     6481 ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc
     6541 agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc agtttgctca
     6601 ggcagctcac cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga
     6661 aataaccggt gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct
     6721 ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat
     6781 agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat
     6841 cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt
     6901 ctgacggtag cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcaggc
     6961 cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac
     7021 cgagcagtaa ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg
     7081 tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca
     7141 cttcacacac tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg
     7201 gcgtgtagcg ggaaacgtgc tctttcagca ggttccacac acagcgcgga tgagtcagtg
     7261 tttcatcgcg ttttgcatag ccgttttcat cgaactgata gttccaggac gacttttcat
     7321 actggcgttt ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac
     7381 gcaccagcag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt
     7441 cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg
     7501 cgtaaatatc cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg
     7561 catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag
     7621 cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca
     7681 tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg
     7741 cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgtgtc agcatcccgg
     7801 tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta
     7861 cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc agctttgcaa
     7921 tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat
     7981 attccgggta gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg
     8041 ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt
     8101 ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac
     8161 agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag
     8221 ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg
     8281 cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt
     8341 attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga
     8401 acatctgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa tagcggtaga
     8461 aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt
     8521 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
     8581 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
     8641 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
     8701 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
     8761 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
     8821 ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat
     8881 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
     8941 cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa
     9001 tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
     9061 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
     9121 gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt
     9181 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc
     9241 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
     9301 tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tatcgttctc
     9361 gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
     9421 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
     9481 tattaaaaaa taaatttcaa ataccagtaa ttcac
//
LOCUS       DAJSKJ010000151         9508 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000151 DAJSKJ010000000
VERSION     DAJSKJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.152"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            478..927
                     /gene="alaE"
                     /locus_tag="ON345_003111"
     CDS             478..927
                     /gene="alaE"
                     /locus_tag="ON345_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR3073135.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(964..1308)
                     /locus_tag="ON345_003112"
     CDS             complement(964..1308)
                     /locus_tag="ON345_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR3073136.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1460..1789
                     /locus_tag="ON345_003113"
     CDS             1460..1789
                     /locus_tag="ON345_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR3073137.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2037..2282
                     /gene="nrdH"
                     /locus_tag="ON345_003114"
     CDS             2037..2282
                     /gene="nrdH"
                     /locus_tag="ON345_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR3073138.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2279..2689
                     /gene="nrdI"
                     /locus_tag="ON345_003115"
     CDS             2279..2689
                     /gene="nrdI"
                     /locus_tag="ON345_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3073139.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2662..4806
                     /gene="nrdE"
                     /locus_tag="ON345_003116"
     CDS             2662..4806
                     /gene="nrdE"
                     /locus_tag="ON345_003116"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR3073140.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4816..5775
                     /gene="nrdF"
                     /locus_tag="ON345_003117"
     CDS             4816..5775
                     /gene="nrdF"
                     /locus_tag="ON345_003117"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR3073141.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6130..7332
                     /gene="proV"
                     /locus_tag="ON345_003118"
     CDS             6130..7332
                     /gene="proV"
                     /locus_tag="ON345_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR3073142.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7325..8389
                     /gene="proW"
                     /locus_tag="ON345_003119"
     CDS             7325..8389
                     /gene="proW"
                     /locus_tag="ON345_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR3073143.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8447..9439
                     /gene="proX"
                     /locus_tag="ON345_003120"
     CDS             8447..9439
                     /gene="proX"
                     /locus_tag="ON345_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR3073144.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDSWIKAHQQQFDGWVNEALAAQK"
BASE COUNT         2371 a         2373 c         2387 g         2377 t
ORIGIN      
        1 attggtgaac agagcggcgc tctctgctcg ctaattccgc aagcctgaca aaaccaaagt
       61 gaataaaaat acaatataac aaaataaaag aaaacatcca tcactggtgc tttctgctgt
      121 taatgtacat tttctggcat aaaaaatgct gtaaatgaca cggtcacgac atacaccgaa
      181 ttctctacgc ttatagcgtt acctcacccc caaactttca ttagtgaaat taaaggcaag
      241 gtcatatcta attgatttaa ttgaaaataa atcatatcgg catgtaaaaa tccacaatgt
      301 acaaaaaacg agccgttacg ggatatttta tctacaaaaa ctgactaaat aaaaattttt
      361 cactaattga atagtcatag ccagcgatat acgctatgcg aaaatgcaga tggcaatgag
      421 atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg
      481 ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac
      541 tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct
      601 ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg
      661 taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat
      721 ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta
      781 gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg
      841 atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc
      901 agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg
      961 tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact
     1021 gaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg
     1081 ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc
     1141 ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata
     1201 gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa
     1261 accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg
     1321 ttattatcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt
     1381 ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg
     1441 aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt
     1501 gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattaaagtc
     1561 ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcattgct
     1621 gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cacgcgatgc
     1681 cgttggctgc gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc
     1741 tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga
     1801 taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa
     1861 tcacaaaaat ccccctaccc cgtcacgctc atatccatgg taatttcgac cactatttgc
     1921 tatatattgt gtggttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     1981 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     2041 gcattactat ttacactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     2101 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcggcagaag
     2161 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     2221 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     2281 gagccagctc gtctacttct ccagcagctc cgaaaatacg cagcgtttta tcgaacgttt
     2341 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     2401 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     2461 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     2521 gggtaatcgc aactttggtg aggcgtatgg ccgtgccgga gatgtgattg ctcagaaatg
     2581 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     2641 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg
     2701 atggattacc acgcgctgaa tgcgatgctt aacctctacg atagcgcagg tcgcattcag
     2761 ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt
     2821 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     2881 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     2941 accagcggtt ttcgtttcca tacattcctc ggggcatgga agttttacac cagctatacg
     3001 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     3061 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg
     3121 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     3181 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     3241 gtaaattccg cactgcaact gtcgaaacgc ggcggcggcg tggcattttt gctgtcgaat
     3301 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     3361 gtgatgaaaa tgctggaaga cgcattttcc tatgccaacc aactcggcgc tcgtcagggg
     3421 gctggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     3481 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgcttggcgt ggtgatcccg
     3541 gatatcactt tccatctggc caaagagaat gcgcagatgg cgctgttttc gccctatgac
     3601 gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa
     3661 ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     3721 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacggtaaac
     3781 cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     3841 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgtacagg ccatgatatt
     3901 tcctgcaatt taggttcgtt gaatattgcc cacaccatgg attcccccaa ctttgcccgc
     3961 acggtagaga ctgccgtacg cggtttaacg gcggtgtcag atatgagtca tatccgcagc
     4021 gtgccgtcca tcgaagccgg aaatgccgcc tcacacgcca tcgggctggg gcagatgaat
     4081 ttacacggtt atctggcgcg agaaggcatc gcttatggtt cgccggaagc actggatttc
     4141 accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggca
     4201 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     4261 ttcagccaat atctgcaagg gaactggcag ccgaaaacgg cgaaagttgg cgaactgttt
     4321 gcccgtagcg gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     4381 atgcgctacg gcatttataa ccagaatctt caggcggtgc cgccaaccgg ttctatctct
     4441 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     4501 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga gaatctggcg
     4561 ctgtatcagg atgcttacga aatcggcgca gaaaagatca tcgacaccta cgcggaagcg
     4621 actcgccatg tcgatcaggg tttgtcgctg acgctgtttt tccccgatac cgccaccact
     4681 cgtgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     4741 attcgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     4801 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     4861 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     4921 gtgccgctgt cgaacgatat tcctgcctgg cagacattaa ctgtcgtaga acaacaactg
     4981 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     5041 ccttcgctga tgcccgatgc actcacgcct catgaagaag cggtattatc gaatatcagc
     5101 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgtcagacc
     5161 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct
     5221 cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     5281 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     5341 ggaaagctga ccaataccgc agatctgatc cgtctgatta tccgcgatga agcagtccac
     5401 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt
     5461 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     5521 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     5581 tacaacgcca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     5641 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     5701 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa
     5761 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat
     5821 tctcgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt
     5881 tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat
     5941 atcaactttt ctcgatttgc tctcagccct tatatcacgg gaaattccgg cgatttgctc
     6001 gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg
     6061 gtagaaaaaa gtgactattt ccattgggta atatatcgac ataaacaaat aaaggaatct
     6121 ttctattgca tggcaattaa attagaaatt aaaaatcttt ataaaatatt tggcgagcat
     6181 ccacagcgag cgttcaaata tatcgaacaa ggactttcga aagaacaaat tctggaaaaa
     6241 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     6301 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     6361 attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgat
     6421 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     6481 atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat
     6541 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     6601 gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     6661 gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca
     6721 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     6781 attgtcttta tttcccacga tctcgatgag gctatgcgta ttggcgaccg aattgccatt
     6841 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg
     6901 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtatt cagtgcgaaa
     6961 gatattgccc gccggacacc gaatggctta attcgtaaaa cccctggctt cggcccacgt
     7021 tcggcactaa aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt
     7081 aataagtttg tcggcgcagt ttccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     7141 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     7201 gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa
     7261 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     7321 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgtgca
     7381 atccgcagac gcctggggta caccggcgac tgcaccgact gacggtggtg gtgctgactg
     7441 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     7501 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt
     7561 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     7621 attgctgctg ggtatgcccg caccggtggc gattatcgtt ttcgctctca tcgcctggca
     7681 gatttccggg gtcggaatgg gtgtggcgac gctggtttcg ctgattgcca tcggcgcaat
     7741 cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaaccgccc tgctgttctg
     7801 tatcgtcatc ggtttgccgt tggggatctg gctggcgaga agtccacgag cagcgaaaat
     7861 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     7921 cgtcatgctg tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgctct
     7981 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
     8041 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
     8101 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
     8161 ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg
     8221 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
     8281 cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
     8341 gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg
     8401 cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt
     8461 actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc
     8521 gggcaaaggt attactgtaa atccagttca aagcaccatc accgaagaaa ccttccagac
     8581 gctgctggtc agccgtgcgc tggagaaatt aggctatacc gtcaacaaac ccagcgaagt
     8641 agattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa
     8701 ctggacgcca ctgcatgaca acatgtacga agctgccggt ggcgataaga aattttatcg
     8761 tgaaggggta tttgttaacg gcgcggcaca gggttacctg atcgataaga aaaccgccga
     8821 ccagtacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga
     8881 taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg
     8941 tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg
     9001 gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt
     9061 ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt
     9121 ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc
     9181 gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg
     9241 gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga
     9301 tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg
     9361 ccacgttgat agctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc
     9421 gctggcagcg cagaagtaat ttttattcag gcggataagg cgtttttgcc tcatccgccg
     9481 ctctatacac aatgcctgat gcgacgct
//
LOCUS       DAJSKJ010000152         9344 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000152 DAJSKJ010000000
VERSION     DAJSKJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.153"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            44..607
                     /gene="srlA"
                     /locus_tag="ON345_003121"
                     /gene_synonym="gutA"
     CDS             44..607
                     /gene="srlA"
                     /locus_tag="ON345_003121"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR3073145.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            604..1563
                     /gene="srlE"
                     /locus_tag="ON345_003122"
                     /gene_synonym="gutE"
     CDS             604..1563
                     /gene="srlE"
                     /locus_tag="ON345_003122"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR3073146.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1574..1945
                     /gene="srlB"
                     /locus_tag="ON345_003123"
                     /gene_synonym="gutB"
     CDS             1574..1945
                     /gene="srlB"
                     /locus_tag="ON345_003123"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3073147.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVVEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1949..2728
                     /gene="srlD"
                     /locus_tag="ON345_003124"
     CDS             1949..2728
                     /gene="srlD"
                     /locus_tag="ON345_003124"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR3073148.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2833..3192
                     /gene="gutM"
                     /locus_tag="ON345_003125"
     CDS             2833..3192
                     /gene="gutM"
                     /locus_tag="ON345_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR3073149.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3259..4032
                     /gene="srlR"
                     /locus_tag="ON345_003126"
                     /gene_synonym="gutR"
     CDS             3259..4032
                     /gene="srlR"
                     /locus_tag="ON345_003126"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR3073150.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4025..4990
                     /gene="gutQ"
                     /locus_tag="ON345_003127"
     CDS             4025..4990
                     /gene="gutQ"
                     /locus_tag="ON345_003127"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR3073151.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4987..6501)
                     /gene="norR"
                     /locus_tag="ON345_003128"
     CDS             complement(4987..6501)
                     /gene="norR"
                     /locus_tag="ON345_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR3073152.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6688..8127
                     /gene="norV"
                     /locus_tag="ON345_003129"
     CDS             6688..8127
                     /gene="norV"
                     /locus_tag="ON345_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR3073153.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8124..9257
                     /gene="norW"
                     /locus_tag="ON345_003130"
     CDS             8124..9257
                     /gene="norW"
                     /locus_tag="ON345_003130"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR3073154.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPAPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKILPM"
BASE COUNT         2218 a         2500 c         2506 g         2120 t
ORIGIN      
        1 ttgccgtttg gtaataaaac aataaatcct gaaggagaga acaatgatag aaaccattac
       61 tcatggtgca gagtggttta tcgggctgtt ccaaaagggc ggagaggtgt ttaccgggat
      121 ggtgaccggc attcttccgc tgttgattag cctgctggtt atcatgaacg cactgattaa
      181 ttttatcggt cagcatcgta ttgaacgttt tgctcaacgt tgcgccggta accctgtttc
      241 ccgttaccta ctgttaccgt gcattggcac gtttgtcttt tgcaatccga tgaccttaag
      301 cctgggtcgc tttatgccgg aaaagtacaa acccagctac tacgcggcgg cctcttatag
      361 ctgccactca atgaatggcc tcttccccca tatcaaccct ggcgaactgt ttgtttatct
      421 tggcattgcc agcggtctga caacgctgaa cctgccactt ggcccactgg cggtgagtta
      481 tctgctggtt ggtctggtca ccaatttctt ccgcggctgg gtgaccgatc tgaccaccgc
      541 catttttgag aaaaagatgg gcattcaact tgaacaaaaa gttcacctgg caggagcaac
      601 atcatgacgc atattcggat cgaaaaagga acgggtggct ggggcggccc gcttgagctg
      661 aaagccacgc cgggaaaaaa aatcgtctat atcaccgccg gtacccggcc tgcgattgtt
      721 gacaaactgg cacagcttac tggctggcag gctattgacg gatttaaaga aggtgaaccc
      781 gcggaggcgg aaattggtgt cgcggtaatc gactgtggcg gcacattacg ctgcggcatc
      841 tatccgaaac gacgtattcc caccattaat atccactcga cgggcaagtc cggtccgctg
      901 gcgcagtaca ttgtggaaga tatttatgtc tctggcgtaa aagaagaaaa catcactgta
      961 gtgggtgatg cgacaccaca accctcttcc gtgggccgtg actatgacac cagtaagaaa
     1021 atcaccgaac aaagcgatgg tttactggcg aaggtgggaa tgggcatggg gtccgccgtt
     1081 gcggtgctgt ttcaatctgg tcgtgacacc atcgacactg tattaaaaac cattttgccg
     1141 tttatggcat tcgtctcggc gctcattggc atcattatgg cttctggcct tggtgactgg
     1201 attgcccacg gtcttgctcc gctggcgagc catccactgg gtctggtcat gctggcgctc
     1261 atctgctcct tcccactgct ttcacctttc ctcggcccag gcgcagttat cgcacaggtt
     1321 atcggcgtat tgattggcgt gcagattggt ctcggcaata ttccgccgca tctggcttta
     1381 ccggcactgt ttgccatcaa cgcgcaggcg gcctgcgact tcatcccggt cggtttgtcg
     1441 ctggcggaag cccgtcagga cacggttcgc gtcggtgtcc cttctgtact ggtgagccgc
     1501 tttttaaccg gcgcaccgac tgtactgatc gcctggtttg tctccggttt tatctatcaa
     1561 tagaggctga aacatgaccg ttatttatca gaccaccatc acccgtatcg gcgcgagtgc
     1621 cactgacgcc ctcagcgacc agatgctcat cacctttcgt gaaggcgcgc cagcggacct
     1681 cgaagagtat tgcttcattc attgccacgg cgagttgaaa ggtgcactcc atcccggttt
     1741 gcaatttacg ctcgggcagc atcgctatcc ggtgaccgct gttggcagcg tggtggaaga
     1801 caaccttcgc gaactgggtc atgtcaccct gcgcttcgat ggtttaaacg aagcggaatt
     1861 tccgggcact gtccatgtgg caggccctgt acccgacgat atcgcgccgg gatcggtttt
     1921 gaagtttgaa tctgttaagg agtaaaaaat gaatcaggtt gccgttgtca tcggtggtgg
     1981 gcaaacctta ggcgcgttcc tgtgccacgg tctggctgcc gaggggtatc gcgtcgcggt
     2041 tgtcgatatt cagagcgaca aagccgcaaa tgtggcacaa gaaattaacg ccgaatatgg
     2101 tgaaagtatg gcgtacggtt ttggtgctga cgccactagc gagcaaagcg ttctggcgct
     2161 ctctcgtggg gtagatgaaa tctttggtcg cgtggatttg ctggtctata gcgccggaat
     2221 agcaaaagca gcctttatca gcgacttcca gctcggcgat tttgaccgtt cgctacaagt
     2281 gaatctggtg ggttatttcc tgtgtgcccg tgaattttcg cgtttgatga tccgcgacgg
     2341 gattcagggt cgcattattc agatcaactc gaaatccggc aaagtgggca gcaaacacaa
     2401 ctctggctac agcgcagcga aatttggtgg cgtcgggctg actcaatcac tggcgctgga
     2461 tctggcggag tacggcatta cggtgcattc actgatgctc ggtaacctgc tgaaatcgcc
     2521 gatgttccag tcactgttgc cacaatacgc gaccaagctg ggtatcaaac cggatcaagt
     2581 cgagcagtat tacatcgaca aagtaccgct caaacgcggc tgcgattatc aagatgtgct
     2641 gaatatgctg ctgttctacg ccagtcctaa ggcgtcgtac tgcaccggac agtcgatcaa
     2701 tgtcaccggc ggtcaggtga tgttctgatc aacagcggag atccattaag gatctccgtg
     2761 agactataga atgcctgatg cgctacgctc atcaggcata caggacttcc gccactacat
     2821 taaggaaaag ttatggtatc cgcactcatc accgtcgccg ttatcgcctg gtgtgcgcaa
     2881 ctggccttag gcggctggca aatttctcgt tttaaccgtg ccttcgacac actatgccag
     2941 caagggcggg ttggcgtggg ccgttccagc gggcgcttta aaccgcgggt cgtggtcgcc
     3001 atcgcgctgg acgatcagca gcgcatcgtc gacaccttgt ttatgaaagg actgaccgtc
     3061 ttcgcccgac cgcaaaaaat tcccgcaatt accggtatgc atgcgggtga tttacagccc
     3121 gatgtgatct ttccccatga tccactatca cagaatgctc tatcattggc gcttaaactg
     3181 aaacgtggat aatttcgttg tgaatgttac ttgcttgcga agttatcatt ttgaaaccta
     3241 aatcaggtaa tcacgcccat gaaacctcgt cagcgtcagg ccgccattct ggagtatctg
     3301 caaaagcagg gtaaatgctc ggttgaagaa ttggcgcaat actttgacac cacaggcaca
     3361 accattcgca aagatctggt cattctggaa catgccggaa ccgtcattcg tacttatggc
     3421 ggagtggtgt tgaataaaga ggaatccgat ccgcctatcg atcataaaac gctcatcaac
     3481 acccacaaga aagagctgat tgcagaagct gccgttagtt ttatccatga tggcgattcg
     3541 atcattcttg atgctggcag taccgttttg cagatggttc ccctgctctc gcgctttaat
     3601 aacatcacgg tgatgaccaa cagcctgcat atcgtcaatg cgctatccga actggataac
     3661 gaacaaacta tcctgatgcc aggcggaacg tttcgtaaaa aatcggcctc atttcacgga
     3721 cagctggcag agaatgcctt cgaacatttc agtttcgata aattgtttat gggcaccgac
     3781 ggcatcgatc tcaatgcggg cgtaaccacc ttcaacgagg tttataccgt cagtaaggca
     3841 atgtgcaatg ccgcgcgcga agtgattttg atggcggact catcaaagtt tggccgtaaa
     3901 agccccaacg tagtttgcag tcttgaaagc gtcgataagc tgattaccga cgcaggtatc
     3961 gatccggcgt ttcgtcaggc gctggaagag aaagggatcg atgtgatcat aaccggagag
     4021 agcaatgagt gaagcactac tgaacgcggg acgtcagacg ttaatgctgg agttgcagga
     4081 agcaagccgt ttaccggaac gtctgggcga tgattttgtt cgcgccgcca atatcatcct
     4141 gcactgcgaa ggcaaagtgg tggtttcggg aattggcaaa tcgggccaca ttggtaagaa
     4201 aatcgccgca acacttgcca gtaccggcac accggctttt tttgtccatc cggcagaagc
     4261 actgcacggc gatctgggga tgattgaaag ccgcgatgtg atgctgttta tctcttactc
     4321 cggtggcgcg aaggaactgg atctgattat tccgcgtctg gaagataaat ctatcgcgct
     4381 gctggcgatg accggcaaac cgacgtcacc gctgggcctg gcggcaaaag cggtgctgga
     4441 tatctccgta gaacgcgaag cctgcccgat gcaccttgcg ccgacctcca gcaccgtcaa
     4501 taccctgatg atgggtgacg cgctggcgat ggcggtcatg caggcgcgcg gatttaatga
     4561 agaagatttt gcccgctccc acccagccgg ggcactgggc gctcgcttgc tgaataaagt
     4621 gcatcatctg atgcgccgtg acgatgccat cccacaggtg gcgttaaccg ccagcgtgat
     4681 ggatgcgatg ctggaactca gccgcaccgg tctggggctg gtggcggtat gtgacgctca
     4741 acaacaggta caaggcgtct ttaccgacgg cgatttacgt cgctggctgg taggcggcgg
     4801 ggcactcacc acgccagtta atgaagcgat gacgacaggc ggcaccacgt tacaggcgca
     4861 aagtcgcgcc atcgacgcca aagagatcct gatgaagcgc aaaatcactg ccgcaccggt
     4921 ggtggatgaa aacggcaaac tcaccggcgc aattaacctg caggatttct atcaggccgg
     4981 gattatttaa tccttcaatc ccagacgttt cgccagccga tgcaggttgg cgacgtcggt
     5041 ttccagcatc cgcgcgcagg cagcccagtt gtgatgattt tgtgccagtg cctggcgaat
     5101 ggtttcacgc tggaacgctt ctgtcgcttc acgcaggttt tgcttaacaa cgggcaccgc
     5161 cgccgcttct ggcggcggca acgtcacctc aggaaaagca aaatgttgcg cctcaagaat
     5221 cacttcatcg ccgttgcggg tggctctcgc cagcactacc gcccgatgaa tagcatgttc
     5281 cagttcgcgc acgttccccg gaaaacgata gtgttgcagt aaatttcgcg ctccggcact
     5341 taataccacg cgggagagcc ccagccgcaa acgacactgc tcgcagaaat accccgccag
     5401 cagaatgaca tcatcgcccc gctcacgcag cggcggcacc gaaagtggaa acacgctcag
     5461 gcgatgaaac aagtcagcgc gaaatcgccc tgccagcacc tcttcgcgta agtcgcggtt
     5521 agtcgccgcc agcacgcgca catcgacccg caaactacgg tcatcgccaa cgcgctgaat
     5581 atcgccatac tgcaacaccc tcagcagctt ggcctgcaat gccaacgaca actcgccgat
     5641 ctcatccaga aacagagtgc cgttatccgc catttcaaac ttcccgctgc ggttactgat
     5701 agcgccagta aacgctcctt tcacatgccc gaacagttca ctttccgcca cactttccgg
     5761 cagtgcggca cagttgagat agaccagcgg attcaccgcc cgtggcgagg cttcatgaat
     5821 cgctttcgcc accagctcct taccggttcc cgtctcaccg ctgatcagaa cgttgagatc
     5881 ggacgccgcc acaatctcaa tctctttttt cagttgcgtc atgccagggg acaagccaat
     5941 catctgcgtc tgtttcaccg cttcaaacgg cgtggcatcg cctggcagca tattctggct
     6001 ttccagttgt tcaatcagca acgcattgct taacgctccc gccgccagcg cggcaatcag
     6061 ccgtaactct tcgtcgctga aaacatcgaa ctgatcgggc tgcatcccgt cgagcgtcag
     6121 tgcgccgatc aggttttgtc cggcaaacaa tggcagacca acgcaggcgt gaaccttcag
     6181 actctcctgc ccaggaatca aaccgtcata gggatcgggc aattcgctgt ctgcaggaaa
     6241 gcgcaccacg tccccggcgc gggcaatcgc ttccagccgt ggatgccctt ccagcgcaaa
     6301 gcgtctaccg agtacatcct ttgccagacc gtcgatggca agcggaataa actgccgcga
     6361 atcgtaacgt agcaacgcag acgcatcgca ctccagcacc tgacgtagcg tggtgatcag
     6421 gcgctgaaaa cgatcctggt gaccaatccc acgctgcaat tcgatggcga tattcgccag
     6481 cacatcaacg gaaaaactca tctttgcctc actgtcaatt tgactataga tattgtcata
     6541 tcgaccattt gattgatagt cattttgact actcattaat gggcataatt ttatttatag
     6601 agtaaaaaca atcagataaa aaactggcac gcaatctgca attagcaaga catcttttta
     6661 gaacacgctg aataaattga ggttgctatg tctattgtgg tgaaaaataa cattcattgg
     6721 gttggtcaac gtgactggga agtgcgtgat tttcacggca cggaatataa aacgctgcgc
     6781 ggcagcagct acaatagcta cctcatccgc gaagaaaaaa acgtgctgat cgacaccgtc
     6841 gaccataaat tcagccgcga atttgtgcag aacctgcgta atgaaatcga tctggcggat
     6901 atcgattaca tcgtgattaa ccatgcagaa gaggaccacg ccggggcgct gaccgaactg
     6961 atggcgcaaa ttcccgatac accgatctac tgtactgcca acgctatcga ctcgataaat
     7021 ggtcatcacc atcatccgga gtggaatttt aatgtggtga aaactggcga cacgctggat
     7081 atcggcaacg gcaaacagct catttttgtc gaaacaccaa tgctgcactg gccggacagc
     7141 atgatgactt acctgacagg cgacgcggtg ctgttcagta acgatgcttt cggtcagcac
     7201 tactgcgacg agcatctgtt caacgatgaa gtggatcaga cggagctttt cgagcagtgc
     7261 cagcgttact acgccaatat cctgacgccg ttcagccgcc tggtaacacc gaaaattacc
     7321 gagatcctgg gctttaactt accagtcgat atgatagcga cctctcacgg cgtggtatgg
     7381 cgcgataacc cgacgcaaat tgtcgagctg tacctgaagt gggccgctga ttatcaggaa
     7441 gacagaatca ccattttcta cgacaccatg tcgaataaca cccgcatgat ggctgacgct
     7501 atcgcccagg gaattgcgga aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc
     7561 cgaagcgata aaaacgaaat cctgaccaat gtcttccgct caaaaggcgt gctggtcggc
     7621 acttcgacga tgaataacgt gatgatgccg aaaatcgccg ggctggtgga ggagatgacc
     7681 ggattacgct tccgtaacaa acgcgccagt gctttcggat ctcacggctg gagcggcggt
     7741 gcggtggatc gcctttccac gcgcctgcag gatgcgggtt tcgaaatgtc gctcagcctg
     7801 aaagccaaat ggcgaccaga ccaggacgct ctggagttat gccgtgaaca cggtcgcgaa
     7861 atcgcccgtc agtgggcgct cgcgccgctg ccgcagagca cggtgaatac ggtagttaaa
     7921 gaagaaacct ctgccacgac gacggctgac ctcggcccac gaatgcagtg cagcgtctgc
     7981 cagtggattt acgatccggc aaaaggcgag ccaatgcagg acgttgcgcc aggaacgccg
     8041 tggagtgaag tcccggataa cttcctctgc ccggaatgct ccctcggcaa agacgtcttt
     8101 gacgaactgg catcggaggc aaaatgagta acggcattgt gatcatcggt tcgggcttcg
     8161 ccgcccgcca actggtgaaa aatattcgca aacaggacgc cactattcca ttaaccctga
     8221 ttgccgccga cagcatggat gagtacaaca aacctgacct cagccatgtt atcagtcagg
     8281 ggcaacgtgc cgatgacctt acccgccaga cggcgggtga atttgccgag cagtttaatc
     8341 tgcacctgtt tccacaaacc tgggtgacgg atatcgatgc cgaagcccgt gtggtgaaaa
     8401 gccagaataa tcagtggcaa tacgacaagc tagtactggc aaccggtgcc agtgcctttg
     8461 tcccgcccgc gcctgggcgt gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg
     8521 cctgtgaaac gcaactgcgg gatgcccgac gcgtgttgat tgttggcggt ggtttgattg
     8581 gtagcgaact ggcgatggat ttttgtcgtg caggcaaagc ggtcacgcta atcgacaacg
     8641 ctgccagtat tctggcgtcg ttaatgccac cggaagtaag cagccgcttg cagcatcggt
     8701 tgacggagat gggcgttcat ctgctgttga aatctcagtt acaggggctg gaaaaaacgg
     8761 attctggcat tctggcaacg ctggaccgcc agcgtagcat cgaagtggat gcggtaattg
     8821 ccgccaccgg actgcgcccg gaaaccgccc tggcacgacg cgccgggctg acgattaatc
     8881 gcggcgtttg cgtcgatagt tatctgcaaa ccagtaatga cgatatttat gcgctgggcg
     8941 attgcgcgga aatcaacggt caggtactgc cattcctgca gccgattcaa cttagcgcga
     9001 tggtgctggc aaaaaatctt ctcggcaata acacgccgct gaaactaccg gcgatgctgg
     9061 tgaaaatcaa aacgccggaa ttaccgctgc atctggcagg cgaaacccag cgtcaggatt
     9121 tacgctggca aattaatacc gaacgccagg gaatggtggc tcgcggtgtt gacgatgctg
     9181 accagcttcg cgcctttgtg gtcagtgagg atcggatgaa agaggcattt ggattgttga
     9241 aaatattgcc gatgtaggtg ggctactgtg cctaaaatgt cggatgcgac gctggcgcgt
     9301 cttatccgac ctacggggac gcatgtgtag gccggataag gcgt
//
LOCUS       DAJSKJ010000153         9284 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000153 DAJSKJ010000000
VERSION     DAJSKJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.154"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..431
                     /gene="rcsA"
                     /locus_tag="ON345_003131"
     CDS             <1..431
                     /gene="rcsA"
                     /locus_tag="ON345_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR3073155.1"
                     /translation="QHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLLISSKSIK
                     PESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISDQMNIKAK
                     TVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(475..663)
                     /gene="dsrB"
                     /locus_tag="ON345_003132"
     CDS             complement(475..663)
                     /gene="dsrB"
                     /locus_tag="ON345_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR3073156.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            826..1053
                     /gene="yodD"
                     /locus_tag="ON345_003133"
     CDS             826..1053
                     /gene="yodD"
                     /locus_tag="ON345_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR3073157.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1351..2166
                     /locus_tag="ON345_003134"
     CDS             1351..2166
                     /locus_tag="ON345_003134"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR3073158.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGGHLHDEDPAHVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2163..3857)
                     /gene="yedQ"
                     /locus_tag="ON345_003135"
     CDS             complement(2163..3857)
                     /gene="yedQ"
                     /locus_tag="ON345_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR3073159.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLISSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     NHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4028..4210)
                     /locus_tag="ON345_003136"
     CDS             complement(4028..4210)
                     /locus_tag="ON345_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR3073160.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(4289..5206)
                     /locus_tag="ON345_003137"
     CDS             complement(4289..5206)
                     /locus_tag="ON345_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR3073161.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5379..6299
                     /gene="yedA"
                     /locus_tag="ON345_003138"
     CDS             5379..6299
                     /gene="yedA"
                     /locus_tag="ON345_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR3073162.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTARPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKSVVAPVIQDASSE"
     gene            complement(6288..6758)
                     /gene="vsr"
                     /locus_tag="ON345_003139"
     CDS             complement(6288..6758)
                     /gene="vsr"
                     /locus_tag="ON345_003139"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR3073163.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6739..8157)
                     /gene="dcm"
                     /locus_tag="ON345_003140"
     CDS             complement(6739..8157)
                     /gene="dcm"
                     /locus_tag="ON345_003140"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR3073164.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8224..8919)
                     /locus_tag="ON345_003141"
     CDS             complement(8224..8919)
                     /locus_tag="ON345_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR3073165.1"
                     /translation="MDLQHWQVQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(8959..9261)
                     /locus_tag="ON345_003142"
     CDS             complement(8959..9261)
                     /locus_tag="ON345_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3073166.1"
                     /translation="MEYGSTKMEERLSRSPGGKPALWAFYTWCGYFVWAMARYIWVMS
                     RIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         2264 a         2506 c         2246 g         2268 t
ORIGIN      
        1 gtcagcacat caagcacatc attaatcaac atcccaatac gttatttatc gtttttatgg
       61 caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaattta ttgataagtt
      121 ctaaatcgat taaaccggaa tctctcgacg atatccttgg cgatattctg aaaaaagaga
      181 caacgataac ctctttttta aatatgccga cgttatcatt gagccgaacc gaatcgagta
      241 tgttgcgaat gtggatggca ggtcagggaa ccattcaaat ctctgaccaa atgaatatca
      301 aagccaagac cgtttcatcg cataaaggta atattaaacg taagatcaaa acgcataata
      361 aacaggttat ctaccatgtc gtccgactga cggataatgt gactaatggt atttttgtca
      421 acatgcgcta acacattctg actggtggtt tcccaccagt caggctgaat aagattactc
      481 tgctttctcc acaaagatac cgtcctgatg ccctgcttca ttaaagaacc agatgccgag
      541 cgggtagtct tccagcgaaa ccaggtacat tgtgccttca ctaaactcct caactgccag
      601 taccacgcca ggacgacgcg gaccgccatc cgttttgact gttacccgat cattcacctt
      661 catcgttttc ctcctgtggc tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc
      721 caggcgcgcg cgagtgctga ttttctcgac ggtctatact taagagatgc caggcggact
      781 taacgactgg cggcaacaac agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa
      841 gagtacagcg ataccgcaaa acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg
      901 atcaatgaaa ttagcgaaag cgaagttcat cgcagccaga acgattctga acacgttagc
      961 gtcgatggac gtgaatatca tacatggcgt gaattggcgg atgccttcga actggatatt
     1021 catgacttca gcgtctctga agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg
     1081 agggggtcgg gatgaaactt gcttaagcaa gaagcactta aaaaattcgt tacaccagga
     1141 aatctgatgt gttcatcacc ttatccgcaa tttttttcgc tgacaagaaa atattcgcta
     1201 tatgaatgat taacgctact tatgaataga aatgtgacgc acgtcacccc ttggtggctc
     1261 tggcgtcagc gggatcgctc ccgaaaaaat catctttgat aatttgtgct gttttgagaa
     1321 tcatcctggt aaggggagaa ctgcgcgacc atgttttcaa ttcaacaacc actactggtt
     1381 tttagcgatc ttgatggcac cctgctggac agtcatagtt atgactggca accagctgcc
     1441 ccctggctca gccgtttacg cgaagcgaat gttcccgtca ttctctgtag cagtaaaact
     1501 tcagcggaaa tgctgtactt gcaaaaaacg ttggggctac aagggttacc gctgattgcc
     1561 gaaaatggcg cagtgatcca gcttgctgaa caatggcagg atatagacgg ttttccacgc
     1621 atcatctcag gtattagcca tggcgaaatc agccaggttt taaatacgct acgcgagaaa
     1681 gaacatttta aattcacaac ttttgatgat gtcgacgatg caaccatcgc cgaatggacg
     1741 ggattaagcc gtagccaggc ggcgctgacg cagctgcatg aggcgtcggt aacgctaatc
     1801 tggcgcgaca gtgacgagcg tatggcacaa tttaccgctc gtctgaacga actgggctta
     1861 cagtttatgc agggtgcgcg cttctggcac gtcctggatg cctctgccgg aaaagatcag
     1921 gctgccaact ggattatcgc gacctatcaa caattgtcag gcaaacgccc aaccacactt
     1981 ggcctgggcg atgggccaaa cgatgcgccc ttactggagg taatggatta cgcggtgatt
     2041 gtgaaagggc taaaccgtga aggggggcat ctgcatgatg aggatccggc ccacgtctgg
     2101 cgaacgcagc gtgaaggacc ggaaggatgg cgtgaagggc tggaccattt tttctccgcc
     2161 cgttaagcgt tatcgctcgc gaatacccga ttacgcccag cctgtttagc gagataaagc
     2221 cgacggtcgg ccagtgactg gagttgttca aaatcataat caccggtttc ctcgctgcta
     2281 cttaccccca acgaggcact gatgcgtatc gtcgtactct tggcaatcaa catctctttt
     2341 tcatttaact tcaggcgaat acgttctgcg acttccgcag cctccgtcag actcgcgcct
     2401 ggcagaatca cacaaaactc ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag
     2461 gaactgctaa ttaatccggc agcatgagaa agaacacggt cgcccgcctg atgaccaaag
     2521 cggtcattaa tcgctttaaa atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg
     2581 tgcgtctgac acaatttagc gagcggacgg gctttttcga acagtgcgcc acgattatat
     2641 aaacgggtta aggtgtcgtg ccacgcctgc cactgcaacg agctttgcag aacatacatg
     2701 ttgctgacca tccggcgaat cacataccag gagatgagta acatggtggt aaagagcgcc
     2761 cacagcaggg ttaatgcaat gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttaac
     2821 gtatggacgc gcaccagcac accgtcgaaa tggttcagac gttcccagct aacatagcga
     2881 ctgtccatac gaatgccgcc ccgcgtgtca tgttccattg cctgcgccag caaggccagt
     2941 tcacgagggt caaaaatatt ccctgttgga tggtcaggat tggaagagat caaaaatctc
     3001 agcttactgt catagagctg atactcacca tcgaggtttt tatcgatggc gtttcttaaa
     3061 aattgctgca tggtacgcac gggaatactc atccccagca cgccatacca gtagttatta
     3121 ctgtctaccg gaacactgac ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc
     3181 gaagtgaacc agcgtaccgc gcggtgacga ttttctcgtt gcgaatggcc gataaaccag
     3241 ggttgggtga catagccgta ataacgcgtt ggtacattgc gcgtaaacaa ggtcggctgc
     3301 gtcgaaacgt aaaatccggc acgcgagaca tacatcgcct gttcaaccat cgacgaggtg
     3361 ttgtgcgcca gtcgcagcaa gtaaccaact tccagtgcag cggtaatttc attgtcgagg
     3421 ctttcatttt cgcgagacag gagattcccc tcgctgacta atgcatccga tacgccattg
     3481 actgacaggg tgcgtcgtcg gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt
     3541 tcgaactcgg ttacagcatc acgcagagag gtgaaatcca gtggcgctac gagagcttcg
     3601 cgcatgccat tacgcaaaaa gatcagtttg tcgacgttat actgcaaatg cttatcgagc
     3661 gcgttggcta cgttttccag atgattacgc tggctggaga tataggcatc ttccagcact
     3721 accacttcac gccaggtcag caaggtggaa aaaagcaata ccacgataaa gcagagatta
     3781 acgacatgac caggccccag gcggcgtgcg agttttttca accagctctg gttttccatt
     3841 tttgtctcgt gctgcaccct gacgactccc aacctgcttt tttatgattc tggcataacc
     3901 tggcgatagc gtaccgcaaa acagcgataa ttttataatg gtggcatgat aagacgcgac
     3961 tatcattcat tgcaggcatt ggcacaccag gccggatgtg atccggcctg gtgttaatga
     4021 taccctttca aacttcttcc gcagaacgac tcccctcccc cggaacaagc tcggtttcat
     4081 gaaaagcttc ccgcttgacc ccgtaaccgt cataccaacg acactcaacc ataccgctgg
     4141 agtatccagt gacaatcatc cgcgggccgc cctctttaac cgtaacttcc tcactaacca
     4201 taaagctcat actcgcctcc ttttttcgag tgaaacttgt tcaccttagt tgaagatggc
     4261 gaattttgca taatagcctg tgcgagtatt aatgtgcctg accgcgtatt ttcgctacgg
     4321 ctttcactcc cagcaccacg atgccgccga tgataaatcc aagaatcaga tttaaaacag
     4381 tcggtaatat catcgccacc actgcacttt gctgcccggc gaaatgttca atggcgtgat
     4441 gcagcggcgc aataccatgt accacaatcc cgccgccgac gaggaacatc gccagcgtgc
     4501 cgacaatcga taatgctttc atcaaccagg gcgcaatgat caataaccct ttacctaatg
     4561 cctgcatcag cgcgctggat ttttccgcca gccaataccc caggtcatca atcttaacga
     4621 taacccctac cagaccgtag acgcccacgg tcactaccag cgcgatgcct gaaagcacca
     4681 gcacctgatt aagcaacggc gcttcggcca caatccccag cgtgatggcg acgatttccg
     4741 cagacaatat aaaatcggta cgaatcgccc cttttatttt gtccttttca aacttcagcg
     4801 gatcctgcgc cgccagcttc tccagacgct gctggctctg cgccggatct tctttatgtt
     4861 tacgcgcctc cagcatatgc agcactttct ctactccttc aaagcagaga aacgcgccac
     4921 caatcatcaa cagaggcgta atcgcccacg ggataaacgc actgatgatc agcgccagcg
     4981 gcaccagaat cactttatta atcagcgatc ctttcgccac gccccagacc acgggaagtt
     5041 cccggttggc ccgcacgcct gaaacttgtt gcgcattgag cgataagtca tcccctaata
     5101 caccggcggt tttcttcgca gccagtttgc ccatcacgga gatatcgtcc agcagtgtgg
     5161 cgatatcatc gagcaacgtc agtaaactac tcccggccaa aagcattccc tctcattttt
     5221 attgttgaag ggtgtaagta tgaagcaaaa acggcagctc tgatacttgc ttcacaggtc
     5281 aacatttttt taacaatcaa gtgcaattat atctctcgcc agcagaatga ttttcacgtt
     5341 tactatgacc caccttttta tttccaccgt gagggattat gcgtttccgc cagttgttac
     5401 cgctttttgg cgcgctgttt gcgttgtata tcatttgggg ctcaacctat tttgtcattc
     5461 ggattggcgt ggaaagctgg cctccgttaa tgatggcagg cgtgcgattc ctggcagccg
     5521 gtattttatt gctggcattt ttgctactgc gcggacacaa actccccccg ctacgtccgc
     5581 tgcttaatgc cgcgctgatt ggcctattat tgctggctgt cggtaatggc atggtgacgg
     5641 ttgccgaaca tcaaaatgtt ccttccggca tcgccgccgt agtggttgca accgtgcccc
     5701 tctttaccct gtgcttcagc cgcctgtttg gcattaaaac gcgcaaactg gaatgggtgg
     5761 gtattgccat tgggcttgcc ggaatcatca tgctcaatag cggtggaaat ttaagcggca
     5821 atccgtgggg cgcgattctg attttaatcg gctcgattag ctgggcgttt ggctcggttt
     5881 atggctcgcg cattacctta cctgtaggga tgatggcggg tgcgattgag atgctggcgg
     5941 caggcgtggt gttaatgatc gcgtcgatga ttgcaggtga aaaactgacg gcgcgccctt
     6001 ccctttcagg cttccttgcg gtcggctatc tggcgctgtt tggttcgatt atcgccatca
     6061 acgcttatat gtatttaatc cgtaatgtca gtccggctct cgccaccagc tacgcctacg
     6121 ttaacccggt ggtcgcggtc ttgctgggta cgggactggg cggagagacg ctgtcgaaga
     6181 ttgaatggct ggcgctcggc gtaattgtct tcgcggtggt actggtcacg ttgggaaaat
     6241 atctcttccc ggcaaaatcc gtagttgcgc cagttattca ggacgcatca agcgagtaaa
     6301 tgaatcccct gcgtgtcgat ctgcgcgctg gcaccttcgc cgcagatcca ctcttccaga
     6361 cgctcggtaa gcgcttcatc cgtcagcttc tcgcgcccac gtaatgcaca ctcccagaca
     6421 atcagtacgc gccagccgag ttcctgcaaa cgactgatat cgcggcgatc gcgttcaaca
     6481 tttttaccta tcttctccag ccagaactcg gttcgagtcg caggcacttt aaacagatag
     6541 cagtgatgat gatgccagaa gcagccatgg gtaaatatca cgcagcgata ttcatcaacg
     6601 acaaaatccg ggcttccggg cagactggcg tcctgaacgc gaaacgccag gccctgcccg
     6661 gttaacagac tggcgaggcg cttctcaatc gccgtatcac gcgtggcaat cgcgcgcata
     6721 tttttgctgc gagtggcctt atcgtgaacg tcggccatgt tgtgcctctt gctgacgcaa
     6781 cgccaccgcc tgtttgattt ttggctcaag cagttttgcc accgcggcaa agaccggcac
     6841 gaccaccgag ttaccgaact ggcgataggc ctgagtgtcc gaaaccggaa tgcggaattt
     6901 cgcttctccc ggcgcttcaa aacccattaa gcgcgcgcat tcccgaggcg ttaaccgacg
     6961 tggacgatgt tgctgattca gcggatcgtc aaaatctttc tcacccgtgg ccatatccca
     7021 gccgcgatcg attaaaattt ccgcgccatc tttgtaataa cgcgcagaca gcgtacgcgt
     7081 gacgctttgc ggattgttcg gataaaccat tccataaccg aagccgttac cgcgcgcctg
     7141 atgttttttc gcatatcgat agaggtactt ccacagcacc ggcgtcagga tatatttcgc
     7201 ctcgaccatc gggtccaaca gctgcgccag cgtcactcgc tgtgcaggga aacattcgct
     7261 gatatcacgc agagtaaaat cggctttcag attaagatcg cgacgaaaac ccaccagcac
     7321 gatgcgttca cggtgctgcg gcagaaaatg tttaccatcg atgattttcg gatcgtccgg
     7381 cccgttatct tctgcatcag ccacgtcata gcccagttcg tccagcgtct gcatgatgat
     7441 gcggaacgtt ttaccctggt cgtgactttt caggtttttg acgttttcga gcacaaacat
     7501 cgccggacga cgcgcgtcga taatgcgtac cacatcaaaa aacagcgtgc cctgggtatc
     7561 gcaggcaaaa ccgtgcgccc gcccgagcga gttctttttc gatacgccag ccagcgaaaa
     7621 tggctgacaa gggaaaccgg ccagtaaaac atcgtgttca ggaatgtgtt gacgaatatg
     7681 ttccgccgcc gcctcatcac tcacgccttc tttatggctg agggtgatat cgcggatatc
     7741 ttcattaaaa tgatgcgtcg ccggatcgca ataatggttg gctttataag tgcgtaccgc
     7801 atgtttgttc cattcgctgg taaacacgca ttgtccgcca atcgattcaa aaccgcgacg
     7861 gatgccgcca attccggcaa atagatcgat aaagcgaaac gcataatgcg gatgatgtgc
     7921 cggtggtttg ggtaacaacg tttgcagatg ggcgaactct ttctcactta aacggtgcca
     7981 tgccgagtca ttcgccagcg cacgttttaa aattgccgcg ctccagtgat tctcacccac
     8041 accattaagc tgcgccacca gcgttttaac atcataaatt tgcagcagtt tctccagcat
     8101 tgcctgtgcg gcattcccgg tgctgtatga atcggttact gatatatttt cctgcataga
     8161 tttaaccggc catctgagat ggccgacagg ttaacataat tacagccatt tagggccacg
     8221 cgcttatccc gcgggtgaaa acgcatctat caccttgtga tcgacacctt cattctcccc
     8281 cgccagttcg gcactgagct tcgccataaa ctcgactaaa aaatgcgcgt tgtgctgcgc
     8341 cagctgcttg cctcttgcgg tttgcatggt ttgcggtagc ttcagcaact tagtctggaa
     8401 atgatccagc gcgtagcgtt tatcatcaag cgggcgatgc tgtgcgaacg gatcttcacc
     8461 atcaaacagc gccacgccca atgcccctga aacggcaaac acgcgcgcca ggccaatcgc
     8521 ccccagggct tccagccgat ccgcatcctg gacgatttta gcttccgttg ttaagggggc
     8581 gatttgcgcg ctgaaactgt gagcggcaat ggcatgacaa acggcctcga ttttctccgc
     8641 cggaaattgc tcgaactctt cacggagcag gcgacgcgtc tcttctgccg ccaggattga
     8701 agaacgctgc ctttgcggat gatttttcgc caggctcaca atatcgtgaa aataacaggc
     8761 ggttaaaatc accagcatat cgacatcgtc atctgccgcc agcttctgtg ccgtagccca
     8821 gacgcggcga aaatgacaca cgtcatgggc cgcgtcctgg tgctgatgat ggtttttcaa
     8881 ccagttttcg aactgtacct gccagtgttg taagtccata cgcgctccgt tgccaaagag
     8941 tcgccacctt agcagttttt attcatagcg tctgctacgt gcgggacgag gtcgggtgta
     9001 ccaggcaatg ctgcctaaca atgccccgac cagagccata aataaaaatc cgaccagtgc
     9061 ccctaaccac tttcccgcgg tagagcctaa atcgctttca aatcccgaca cgggggcatc
     9121 ggggatccgg ctcatcaccc agatgtaacg cgccattgcc cagacgaaat agccacacca
     9181 tgtatagaaa gcccacaaag ccggtttgcc gccagggctg cgagagagtc tctcttccat
     9241 ctttgtcgaa ccgtattcca ttttgagata acccggaaaa ttca
//
LOCUS       DAJSKJ010000154         9280 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000154 DAJSKJ010000000
VERSION     DAJSKJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.155"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            210..1523
                     /gene="gltP"
                     /locus_tag="ON345_003143"
     CDS             210..1523
                     /gene="gltP"
                     /locus_tag="ON345_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR3073167.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1601..2290)
                     /locus_tag="ON345_003144"
     CDS             complement(1601..2290)
                     /locus_tag="ON345_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR3073168.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2384..4531)
                     /gene="fdhF"
                     /locus_tag="ON345_003145"
     CDS             complement(2384..4531)
                     /gene="fdhF"
                     /locus_tag="ON345_003145"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4112..4114),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR3073169.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4729..6195)
                     /gene="mdtP"
                     /locus_tag="ON345_003146"
     CDS             complement(4729..6195)
                     /gene="mdtP"
                     /locus_tag="ON345_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR3073170.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6192..8243)
                     /gene="mdtO"
                     /locus_tag="ON345_003147"
     CDS             complement(6192..8243)
                     /gene="mdtO"
                     /locus_tag="ON345_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR3073171.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRLGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVELERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8243..9274)
                     /gene="mdtN"
                     /locus_tag="ON345_003148"
     CDS             complement(8243..9274)
                     /gene="mdtN"
                     /locus_tag="ON345_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR3073172.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASTMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
BASE COUNT         2046 a         2508 c         2498 g         2228 t
ORIGIN      
        1 attaatcctg ccataaactc gcgttattat gcattaatgc agcgaaaagc tctgttgtta
       61 aagggttgcg caacataccg cgcaaatgat actgatcata agcgttaaaa aaatctacaa
      121 accaacgcaa cacaattcat gccctggcag tatgtcacgt tctcgcgttt ctgaacgggg
      181 aacggcgctc cattgaggaa gttattctga tgaaaagtat aaaattcagc ctggcctggc
      241 agattctgtt tgctatggtg ctgggcattc tcctgggaag ctacctgcac taccatagcg
      301 acagccgcga ctggctggtc gtcaatttgc tctctccggc gggtgatatc ttcatccatc
      361 tgatcaaaat gattgttgtg ccgattgtga tctccacgct ggtggtgggt atcgcgggtg
      421 ttggtgatgc caaacagctc gggcgtattg gcgcgaaaac cattatttac ttcgaggtga
      481 tcaccaccgt cgccatcatt ttggggatca ctctggcgaa cgtcttccag cccggtgccg
      541 gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc actacggaag
      601 cggtacaaag cagttcccac ggcattatgg gcacgatttt gtcgctggtg ccgacgaaca
      661 ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat cttcttctcg gtgctgtttg
      721 gtctggggct ttcctccctg cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct
      781 ccatctctga aaccatgttt aaagtgactc acatggtgat gcgttatgca ccggtgggtg
      841 tgtttgcgct gattgcggtg acggtggcta actttggttt ctcttctctg tggccactgg
      901 cgaaactggt gctgctggtg catttcgcca ttctgttctt cgcgctggta gtgctgggaa
      961 ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg aaagatgagc
     1021 tgattctggc gtactccact gccagctctg aaagcgtact gccgcgaatt attgagaaga
     1081 tggaagccta cggagcaccg gcgtcgatca ccagcttcgt ggtgccgacc ggttactctt
     1141 ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc tattttcatc gctcagctgt
     1201 atggcattga cctgtccatc tggcaggaaa tcattctggt gctgacgctg atggtgacct
     1261 cgaaagggat tgctggcgtg ccgggcgtgt cgtttgtggt gctgctggca acgctgggta
     1321 gcgtaggtat tccgctggaa ggcctggcgt ttattgctgg tgttgaccgt atcctcgaca
     1381 tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg attgccaagt
     1441 gggaacacaa atttgaccgc aaaaaggcgc tggcttatga gcgtgaagtg ctgggcaaat
     1501 ttgataaaac tgcggatcaa taattgaaga ttgccggggt tatccacccg gcaatgtgtg
     1561 gatgcctgat gcgacgctgg tgcgtcttat caggcctacc ttaaccgttg gttaattttt
     1621 caaactcttc acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg
     1681 ccttttgttt gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc
     1741 agtactcgga ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct
     1801 tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg
     1861 catcgacggc agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatcgcct
     1921 ttggataatc cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac
     1981 cttctttact gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca
     2041 gactgaccgg attggtgatt ttgatctgcg ccaacagcgc gcaggcatca gcatcaccgc
     2101 tgtcggcggc tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc
     2161 tgtcggcaag aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc
     2221 tgccaggctc ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa
     2281 tttttttcat tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg
     2341 ataaaaaaac agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt
     2401 cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct
     2461 gatcggcgat cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg
     2521 ggcttaagtt ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt
     2581 aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac
     2641 gcgagtgcac ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat
     2701 tgatttgtgc gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccagtca
     2761 tcgaacggca agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt
     2821 cggtgagttt gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg
     2881 gggtatcaaa cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc
     2941 ggcaaggcca ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat
     3001 ccgggcacag atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat
     3061 aacccatacg ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg
     3121 gttcaactgc tttgaagaaa cgctggaaac cacggtcagc cgcagtaaac acgccttcat
     3181 gctcgcccca cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa
     3241 agatgtcctg aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct
     3301 ccgcgtcagt ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg
     3361 ctgcgcggtg cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc
     3421 cccaggcttt ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat
     3481 acgtatccgg cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg
     3541 ggtttacacc cgcatgcggc ttaccgaggt taccggtcag catcgcgagg ctggtcagag
     3601 aacgcacggt ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg
     3661 cgcttttcgc ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg
     3721 tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc
     3781 cttctgtacg ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc
     3841 ccatcgcatt caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa
     3901 tgcgcgcggt ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag
     3961 cgttaattac gtgattcgcc acgattgggt gggaatccgc cgggttgtac ccgaaaacga
     4021 acactaaatc ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg
     4081 attggtgcag acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat
     4141 tggtaccaat aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac
     4201 cacgcgagga gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca
     4261 ggcgctcggc aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac
     4321 gctggcgacg gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga
     4381 aatcccagcc ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg
     4441 cctccgcccg gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc
     4501 aataggggca aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc
     4561 atacgcgctc tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc
     4621 tgatattacg ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg
     4681 acgtgacgaa acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc
     4741 gacgacggga gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat
     4801 cacccggcgg ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc
     4861 ttcagtggcc tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg
     4921 ggtgaagcgc gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg
     4981 cagacgcgtg ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg
     5041 ttcaatcatc atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc
     5101 gtcaaacagc ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa
     5161 taaggtatcc aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta
     5221 gaacagcgcc cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc
     5281 ttgcagatcc gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg
     5341 gactcgtggt aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa
     5401 tgcacgcaga gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc
     5461 gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc
     5521 cactttactc tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag
     5581 atcgagcatc tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag
     5641 cgatagctct actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc
     5701 aaccgctgag cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt
     5761 ggcttccgta tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct
     5821 taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa
     5881 ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc
     5941 ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctgcgggtc
     6001 attgagttgt ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcgtc
     6061 ggccagtttg atttgttccg gtttgagctg ttgatgaggt gcagaatcct tacgtaccag
     6121 ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag
     6181 ttgacgattg atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg
     6241 gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag
     6301 gtgggcagga tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg
     6361 agtgcggttg ccagaccggc agcgtatttc tccagggcgt cggcaaactg acctgctctg
     6421 tccggctgta gtgcgttatc cagcgcctgc gccgagttcc aggtggcatc ccaggcgtga
     6481 agaatatcgc ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct
     6541 tcgctgtcca gttgacgctc cagctccacg cgttggcaca tctcttcaca ggcattaaac
     6601 gccgcatgca gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgc
     6661 cgtgggatcc gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc
     6721 gtgcgcgctt cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca
     6781 atgatgatac ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg
     6841 ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt
     6901 tcagagctcg tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat
     6961 tcgacaatat tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg
     7021 ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttggg
     7081 ttagcgacga tcacgcatgt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg
     7141 taaaaggtgt aacagatcaa acaggcgagc agcgttttta ccgcgtagcg catatagtct
     7201 ggattggtaa aagcatcggc ggccattgat ggcggtttgg ctgcgggcgt tggcggcgta
     7261 ttcgggtcca tctgacccag ttgtaacaac gtctggcaga tattctccag gttacattcc
     7321 cgtgccgcca tcgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca
     7381 gcaacggcat gctgcagctt attgatttct gaagctaatt tttgtcggaa ttcaataatt
     7441 gcctgagaat cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt
     7501 accgttgcca cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg
     7561 agacaaaaga cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt
     7621 agcggtgcga ggctgtccgt caggtggcta atggcatcat caagccgatc attaagcgcc
     7681 tgatgcattt gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc
     7741 aaggttggat agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga
     7801 tagtcgagca tggcggggaa ggtttgcccg taaatggcga caatggcgac tgcgaaaaag
     7861 accagtccca gccgatgggt gcgcatcaaa aacatacagc ccatcaggat cggcccggcg
     7921 atgatcaatc ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg
     7981 atctccagca tcgtggcgac cacaaataat atcgcgacaa atttggtgta aaacgcgttc
     8041 gactgaatac cgtaaaacag cactgccagc gataacgcca cgaaagggat ctcaaaggtc
     8101 atcgagatca gaatcaccag caggcagcct acccagagtt gcacggtctg cggcacgcgg
     8161 cctagtcgcc gctcgcttaa ctcttcatga aagaacgcca gcagcctgac caccggtaat
     8221 ggcagggagt tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga
     8281 tgcggaacat ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa
     8341 cgcggaccca gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt
     8401 catccggtag cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt
     8461 cactcatcag gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc
     8521 ggaagttggc gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatcg
     8581 tagatgcaaa ctgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc
     8641 gaacggtcgc catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac
     8701 gctgggcaac taatgcatcc acgccgctga cggcgctggc ggctgactgc gcctgcaaca
     8761 atacagcatt aagatcggct tctgcagcgc gctgcgcggt cctcgcacgg tcaacgtctt
     8821 ccgctgagac aaaaccttct ttcagtaatg gctcggtgcg gcgtaatgta tctgtggcct
     8881 gtttcgcggc ggcacgggct ttttctaccg tggcgttaac cgagtcggca ccaaactgtt
     8941 gcgcgtcaac gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt
     9001 cagctttcgc cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct
     9061 gtttgactgc ctggttgtcg gtgaccgcca gttctacaat gcgaccgctg acttccggca
     9121 ccacatcaat ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc
     9181 gccagataac gaaaacaagg gcaaccagcg ccaacgcaac cactaacaga gcagggaatt
     9241 tactgcgagg agcttttttc ggagtacttt ccatgagcgc
//
LOCUS       DAJSKJ010000155         9276 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000155 DAJSKJ010000000
VERSION     DAJSKJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.156"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(97..1287)
                     /gene="bcr"
                     /locus_tag="ON345_003149"
     CDS             complement(97..1287)
                     /gene="bcr"
                     /locus_tag="ON345_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR3073173.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1315..2010)
                     /gene="rsuA"
                     /locus_tag="ON345_003150"
     CDS             complement(1315..2010)
                     /gene="rsuA"
                     /locus_tag="ON345_003150"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR3073174.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2160..3920
                     /locus_tag="ON345_003151"
     CDS             2160..3920
                     /locus_tag="ON345_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3073175.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIIEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4045..4329
                     /gene="rplY"
                     /locus_tag="ON345_003152"
     CDS             4045..4329
                     /gene="rplY"
                     /locus_tag="ON345_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR3073176.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4468..5475)
                     /gene="yejK"
                     /locus_tag="ON345_003153"
     CDS             complement(4468..5475)
                     /gene="yejK"
                     /locus_tag="ON345_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR3073177.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQHRTSGG
                     N"
     gene            5657..5884
                     /locus_tag="ON345_003154"
     CDS             5657..5884
                     /locus_tag="ON345_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR3073178.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5904..7664
                     /gene="pbgA"
                     /locus_tag="ON345_003155"
     CDS             5904..7664
                     /gene="pbgA"
                     /locus_tag="ON345_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR3073179.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7739..7815
                     /locus_tag="ON345_003156"
     tRNA            7739..7815
                     /locus_tag="ON345_003156"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7773..7775,aa:Pro,seq:ggg)
     gene            8022..9263
                     /locus_tag="ON345_003157"
     CDS             8022..9263
                     /locus_tag="ON345_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3073180.1"
                     /translation="MARKVTPLTTTQIKAAKPAEKEYTLQDGGGLFLVIKPSGSKLWR
                     FNYYRPSNKKRTLISLGSLDEVSLADARKRRSEYRTLIAAGTDPQEYERQKREAEARR
                     QGNTFENVAALWYEMKKNQNLAHNTIKDIWRSLEKYVFPYIGNTPIDTLTARRFVEIL
                     TPIKARGNLETLKRVLQRINEVMDFAANSGMIDINTAANVRKTFPSPTKKHMPTIRPE
                     QLPQLMHDLSIASIERQTRLLIEWQLLTATRPAEASAARWEEINLEAATWTIPAGRMK
                     MRRDHVIPLCAQAMAVLEAMKPISARREHVFPSLKNPVQPMSSQTANAALRRMGYTGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYLEKRKVLMCWWGD
                     FVEAAATGTTIASGHRGLRGR"
BASE COUNT         2395 a         2447 c         2313 g         2121 t
ORIGIN      
        1 ttttacataa aataaacata tatcggggaa atgatagatt tgtggttgac atgcatcaac
       61 aaaggaagcc ttttaccttc ctcgttgtgc aatagatcac cgttttttcg gccgactggc
      121 gtacagacag aagagaatgg agctggttgc gcagaatgca attgaccaaa tcatcggcca
      181 tgcagagtta aaggtcgcaa gagaaagcaa tgcgccaaca attgccccta tgccaaaacg
      241 gaaggttcct gccagcgaag atgccgttcc cgccatatgg ggaaactcat caagaatgac
      301 cgccatcgca ttggatgaca ccatcgacac gcagcccaca aacgccgcaa cgccaaccac
      361 cagcgaccag aatcccagcc ccagcagcgc actgatgacc atccacgctg ccataataaa
      421 ttgtatccac aaccccgagc ggaacatatt taacgcgcca atgcggcgga cgaagcggct
      481 gttaaagatg gtcatcacga acagaaaaac aatgtttagc gcaaagtaat aaccaaagtt
      541 ttccggcgcg acgtggttaa tttcaatata aacaaacggt ccggcgctta agaatgagaa
      601 catcccggca aagctgaaac cactggcaag catgtagctc aggacacgtt tatggcggaa
      661 cagcgccgca aagttaccaa tagtagtgcg aatgtgaaaa ggctggcgac gctccggtgg
      721 taaggtttct ttgatcagaa agaagatcat tgccgaagcc agaatcgccg ccacggcgag
      781 gatccagaag atgtaatgcc agctcagcca caccagcacc cagccgccaa ctatcggtgc
      841 catcagcggt gcaatggtcg tcaccagcat gacaaacgac atcatccgcg agaactcttc
      901 tttcgggtaa atatcgcgca tcagggcgtt aatgaccacg ctggccgcag ccgcagccag
      961 cccgtggaag aaacgcatca caatcagctg atcgatggtg tttgccaacg cacacgccac
     1021 cgcggcggcg gcaaacacca gcgtaccgcc gagcaccacc ggcttacgcc cgaagctgtc
     1081 tgccatcggc ccgtagatta actgccccaa cgcaaagccc agaatataag tactgagggt
     1141 catctgcgta ctgcccgccg atacgccaaa ctgcgctgaa attaccggta gcgcgggcag
     1201 atacatatca atcgacagcg gcatcaacat ggccagcagg ccaaggataa aaacaatagc
     1261 aaacgacgaa tgctgtcggg tggtcacaac gggctcctga aagtcatgga aaagttagac
     1321 gacgctggca atttcttctt cagttaacgg acgatattca ccgggggcta aatcagcatc
     1381 cagcgtaatg ccgccaatac gttcacgatg cagctcaacc acgtggttac ccacggcggc
     1441 gaacatgcgt ttcacctgat gataacgccc ttcactgatg gtcagacgga cctgcgtagg
     1501 ggtaatcact tccagcaccg caggcttagt gagatctttt tcgttatgca gctgcacgcc
     1561 tttagcaaat tgctctgccg tatcgtcagc tacaggtgat tccagtgtca ccagataggt
     1621 cttctcgcaa tgatggcgcg gagaagtaat gcggtgcgac cactgaccat catcagtcat
     1681 cagcaccaga ccggtggtat cgatatccag acgccctgcg gcgtgcagtt tccacgctac
     1741 cggttcatca agaaaataga gcaccgttgg gtgatccgga tcgtccgtgg agcaaacgta
     1801 gccctgaggc ttattgagca tgaagtaacg tggaccgtgt tgctgcgcca gcgggttgcc
     1861 atcgtaagcg acatcatgtt cagggagcag tttgaacgct gcattacgaa cgatttcgcc
     1921 atcgacggtg acacgattgc cgcggatttc acgcccggca atagcacggc taacgccgag
     1981 ttgctgtgcg ataaatttat caagtcgcat gtgtgtgatt ttgcctgtaa aaaacggagg
     2041 tcgggcaatg acccgaaaat ctgaactgtt gtctgcccag tatagcggtc taataacttc
     2101 cctcaagcga aaaagaaccg tggcatacta ttagcagaat aatctaccta cgcgagacca
     2161 tgatttttac actccgccca tatcagcaag aagccgtgga tgccacgctc aaccattttc
     2221 gtcgtcataa aacccctgcc gttatcgtgc tgcccaccgg cgcaggtaaa agcctggtga
     2281 ttgccgaact ggcacggctg gcgcgtggtc gtgttctggt gctggcacac gtcaaagaac
     2341 tggtggcgca aaaccatgca aaatatcagg cgctggggct ggaagccgat atttttgccg
     2401 ctgggttaaa gcgcaaagag agccacggta aagtggtatt tggcagcgtg cagtcggtcg
     2461 ctcgtaatct tgatgccttt cagggtgaat tttcgctgtt gattgtcgat gaatgccacc
     2521 gtattggtga cgatgaagag agccagtatc agcaaatcct cactcacctg actaaagtga
     2581 atccccactt acgcctgctg gggctgactg ccacgccttt tcgattgggc aaaggctgga
     2641 tctaccagtt ccattatcac ggcatggtac gcggcgatga gaaagccctt ttccgtgact
     2701 gcatttatga gctgccgctg cgttatatga ttaaacacgg ctatctgacg ccgccagaac
     2761 gactggatat gccagtagtg caatacgatt tcagccgctt gcaggcacag agtaacgggc
     2821 tgttcagcga agctgatctc aaccgtgagc tgaaaaaaca gcaacgcatt acgccacata
     2881 tcatcagcca gatcatcgag ttcgccgaaa agcgcaaagg ggtaatgatt ttcgccgcca
     2941 ccgttgaaca cgcaaaagag attgtgggat tactgcctgc cgaagatgct gcactgatta
     3001 ctggcgacac ccccggcgct gagcgcgatg tgttaattga agattttaaa gcccagcgtt
     3061 ttcgctatct ggtcaacgtc gcagtactga ccaccggatt tgacgccccg cacgtcgatc
     3121 ttatcgccat tctgcgccct accgaatcag tgagtcttta ccaacaaatt gtcgggcgcg
     3181 gtctgcgtct cgctccgggc aagactgatt gtttaatcct tgattatgcg ggtaatcctc
     3241 acgatctcta cgcgccggaa gttggtacac cgaaaggcaa aagtgacaac gttccggtac
     3301 aggttttctg ccctgcctgc ggttttgcca acaccttttg ggggaaaacg accgccgacg
     3361 ggacattgat tgaacacttt ggtcgccgct gtcagggatg gtttgaagat gacgacggtc
     3421 atcgcgaaca gtgtgacttc cgtttccgtt ttaaaaattg cccgcaatgt aacgcagaaa
     3481 atgatattgc cgctcgccgc tgccgcgaat gtgacacggt actggttgat ccggatgata
     3541 tgttaaaagc ggcgctacga ctgaaagatg cgctggtatt acgctgtagc ggcatgtctt
     3601 tgcagcatgg gcacgacgag aaaggcgaat ggttgaaaat cacctattac gatgaagacg
     3661 gcgcggatgt gagtgagcgt ttccgtctgc aaacgcccgc ccagcgaact gccttcgagc
     3721 agctttttat ccgcccgcat acgcgcacac cgggcatccc tctgcgctgg atcaccgccg
     3781 ccgatatcct cgcccagcaa gccttactgc gacatccgga ttttgtcgtc gcccgcatga
     3841 aaggtcagta ctggcaggtg cgtgaaaaag tgttcgatta cgaaggtcgt tttcgtcggg
     3901 cgcacgaatt acgcggttaa tggtagtttt gattgatgta taaggcgatt gagtatagaa
     3961 tctcgcccgc ttttgcatac gcaaagcaga tcacttacct gttgctgggt cgcctgtagc
     4021 aggattaatt taagagagaa agaaatgttt actatcaacg cagaagtacg taaagagcag
     4081 ggtaagggtg cgagccgccg cctgcgtgcc gctaacaagt tcccggcaat catctacggt
     4141 ggcaaagaag caccgctggc tatcgagctg gatcacgaca aagtcatgaa catgcaagct
     4201 aaagctgaat tctacagcga agttctgacc atcgttgttg acggtaaaga aatcaaagtt
     4261 aaagctcagg acgtacagcg tcacccgtac aaaccgaagc tgcagcacat cgacttcgtt
     4321 cgcgcttaat tgctgaataa gttgtaaaaa accccgctcc ggcggggttt tttgtatctg
     4381 cagattatgc ctgattacgg tattgctatt ttttgcaggc cagataaggc gttcacgccg
     4441 catctggcat tcagaacaca aaaccgatta attgccgcca gacgtccggt gctgcaattg
     4501 gtcgcgcaaa ttcggcggcg tgcctttaat ggtcaaggta tcggttgctg gatcccagaa
     4561 gatgcgttca cctaacagca tcgcatcaaa gttaatagtc aagccaccgc cgctaccggc
     4621 aaacttcgtc aactgacgca gtgtgctacg gtctgccggg aagctctctt ccagttcgta
     4681 gcctttttca gccgcaaact ccgtgaagct cacttcgctt acgcctgcca gctctttcga
     4741 cagcgattcc agttcgatct cttcgcctgc ctgcagctgc tcattacagt agctataaac
     4801 ctgctggcgc acgttctgtc gctctgcttt atccagttgt gcttcggcag tgaaatcatc
     4861 aaccgcttgc aacagcccac ggttttgcgc tttcgcgttc aatccttcgc tggccccgag
     4921 gaaatccata aagaaatccg ccactttgcg ccctactcgc cctttcagga aagtgagata
     4981 gcgggtggac tctggattgg tttcccattc ggttaaatcg atacgcgcaa caatatccgc
     5041 atgattgata tcaagataat gggttgggtt gatgtcgaga ttttcgttaa cgcgcatact
     5101 gctcaggttg ctcagaactg ccaccagcag atactccacc gccagataac ggtagtggca
     5161 aaacagcacg aatccaccgt cagcaaaagg atatttcgct aattcgtcac gcaggcgccc
     5221 ggttgccgcg cggctaaatg ccagaaagtc ctcttcgccc tgacgctgta agcgcagcgt
     5281 ctgcgccagt tcactctctt cgctaaacaa gccgtaagct ttatttttgg cgctatagac
     5341 ccgatgcagt tctgccacca tctcaacgac ggtttctgtc ggttccagca atgaatcgcg
     5401 caacaccagc tcaaggttct gctcatcacg cttgataagc tggtgcaggg caatctggtt
     5461 gatatccaga ctcatgataa actctccttt aagaccgggc ggtattcaac caccgccggt
     5521 taagcgacgc aataaaagat ggcggtcatt ttacagaagg cagcgtaacg agaatgattt
     5581 aacgcataaa aaagcagaaa aaaaaccgtt gctacggtaa tatgttgccc tttcatcaac
     5641 taactgattt cgatttatgc cacaaatttc ccgctacagt gatgaacagg ttgaacaatt
     5701 gctcgcagag ctgctcaacg tactggaaaa acataaggct ccgaccgacc tttccctgat
     5761 ggtgttaggc aacatggtca ctaaccttat caacaccagc attgccccgg cccaacgcca
     5821 ggcaattgcc aactcttttg cccgcgcctt acagtcctct atcaacgaag acaaagcgca
     5881 ctaagggaaa cagataacag gttatggtaa ctcatcgtca gcgctaccgt gaaaaagtct
     5941 cccagatggt cagttggggg cactggtttg cactgttcaa tattctgctt tcgctcgtca
     6001 ttggcagccg ttacctgttt atcgccgact ggccgacaac gcttgctggt cgcatttatt
     6061 cctacgtaag cattatcggc catttcagct tcctggtgtt cgccacctac ttgctgatcc
     6121 tcttcccgct gacttttatc gtcggctccc agaggctgat gaggtttttg tccgtcattc
     6181 tggcaacggc gggaatgacg ctattactga tcgatagcga agtctttact cgtttccatc
     6241 tccatcttaa tcccatcgtc tggcaactgg ttatcaaccc agacgaaaat gagatggcgc
     6301 gcgactggca gctgatgttc atcagcgtgc cggttatttt attgcttgaa ctggtgtttg
     6361 cgacgtggag ctggcaaaag ctgcgcagcc tgacgcgtcg tcgacgcttc gcgcgcccgc
     6421 tggccgcatt cttatttatc gcctttatcg cctcgcatgt ggtgtatatc tgggccgatg
     6481 ccaacttcta tcgcccgatc accatgcagc gcgctaacct gccgctttcg tacccgatga
     6541 cggcgcgacg ttttcttgag aagcatggtc tgcttgatgc gcaggagtat caacgccgtc
     6601 ttattgagca aggtaatcca gacgccgttt ccgtacagta tccgttaagc gaactgcgct
     6661 atcgcgatat gggcaccggg cagaatgtgc tgttgattac tgtcgatggc ctgaactact
     6721 cacgcttcga gaagcagatg cctgcgctgg caggttttgc tgagcaaaat atttcgttca
     6781 cgcgccatat gagctccggc aacactacag acaacggcat ctttggcctg ttctatggca
     6841 tctcgccgag ctatatggac ggcattctgt cgacccgtac gcctgcggca ttaattactg
     6901 cgcttaatca gcaaggctat cagctggggt tattctcgtc agatggcttt accagcccgc
     6961 tgtatcgcca ggcattgttg tcagatttct cgatgccgag cgtacgcacc caatccgacg
     7021 agcagaccgc cacgcagtgg atcaactggc tgggccgcta cgcacaagaa gataaccgct
     7081 ggttctcgtg ggtctctttc aatggcacta acattgacga cagcaatcag caggcatttg
     7141 cacggaaata tagccgggcg gcaggcaatg tcgatgacca gatcaaccgc gtgctcaatg
     7201 cactgcgtga ttctggcaaa ctggacaata cggttgtgat tatcactgcc ggtcggggta
     7261 ttccgctgag cgaagacgaa gaaacctttg actggtccca cggtcatctg caggtgccat
     7321 tagtgattca ctggccaggc acgccggcgc agcgtattaa tgcgctgact gatcataccg
     7381 atctgatgac gacgctgatg caacgcctgc tacatgtcag cacacctgcc agcgaatatt
     7441 cgcaaggtca ggatttgttc aaccctcaac gccgtcatta ctgggttact gcagcggata
     7501 acgatacgct ggcaattacc accccgaaaa agacgctggt gctgaacaat aacggtaaat
     7561 accgcactta caacttacgt ggtgaaagag tgaaagatga aaaaccacag ttaagtttgt
     7621 tattgcaagt gctgacagac gagaagcgtt ttatcgctaa ctgattaatt ataaatcagt
     7681 tagcgaaata tcttacttgc aatcggtgtg gaaaacggta gtattagcag ccacgagtcg
     7741 gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc gggggtcgga ggttcaaatc
     7801 ctctcgtgcc gaccaaatca actatagcta atcatcgttt ttatgaaaaa agctcgctaa
     7861 ttcattgaat agcgggtttt tttattgttt ttagtcatcg ttattcgacg gtaacagtgg
     7921 ttttcagccg ttttttttag ttatatgttt agttatacgg gctggcgcat agctaaatcc
     7981 gtataactag aacctgtttt tccccgtgga ttcataaacg tatggcaaga aaggtaaccc
     8041 cactcaccac cacacagatc aaagcagcta aaccagcaga aaaagaatac accttacagg
     8101 atggcggcgg gctttttctc gttattaagc cgtctggttc gaaactatgg cgattcaact
     8161 actatcgacc ttcgaacaaa aaacgaacac tcattagttt gggatcgctt gatgaagtct
     8221 cccttgctga tgccagaaaa cgccgtagcg agtacaggac gttaattgcg gcaggaaccg
     8281 acccgcagga atacgaacgg caaaaacgcg aagcagaggc tcgacgacag ggcaacacgt
     8341 tcgaaaatgt ggccgcattg tggtacgaaa tgaaaaaaaa ccagaatctc gcccacaata
     8401 cgatcaagga tatctggcgc tcgctagaga aatatgtatt cccgtatatc ggaaacacac
     8461 cgatagatac tctcaccgct cgccgctttg ttgagatact cacgcccatc aaggcacgtg
     8521 gcaacctgga aacactgaaa cgcgttttac agcgcatcaa tgaagtgatg gattttgctg
     8581 ccaacagtgg aatgattgac atcaacaccg ccgcgaacgt tcgcaagaca ttcccttctc
     8641 ccaccaaaaa gcacatgcca accatccggc cggaacaact accgcagcta atgcacgatt
     8701 tatcgatcgc cagcatagaa cggcaaacca gattactgat tgagtggcag ttgttaaccg
     8761 caacccgccc agccgaagcc tctgccgcac ggtgggaaga aatcaacctt gaggcggcaa
     8821 cctggacgat accagccgga cgcatgaaga tgcgccgtga tcatgtgatc cccctttgcg
     8881 ctcaggcgat ggcagtgctt gaagccatga agccgattag cgcacgaagg gagcatgttt
     8941 tcccaagtct taaaaatcca gtgcaaccaa tgagcagcca gacagcaaac gcagcattac
     9001 ggcgaatggg ttacactggt gtgctggtat ctcacggact acgcgccata ttcagcacag
     9061 cggcgaacga agaaggattt gagccggacg taatcgaggc cgcactcgca cacgtggaca
     9121 cgaacgaagt tagacgggca tacaaccgaa gcaactacct ggaaaaacgt aaagtgttaa
     9181 tgtgctggtg gggtgatttt gtggaagcag ccgcaaccgg aacaaccatc gccagcgggc
     9241 acagaggatt acgcggaagg taatgactcc aactta
//
LOCUS       DAJSKJ010000156         9204 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000156 DAJSKJ010000000
VERSION     DAJSKJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.157"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(135..464)
                     /locus_tag="ON345_003158"
     CDS             complement(135..464)
                     /locus_tag="ON345_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR3073181.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKSGEYVQGIN
                     GKWVRKF"
     gene            complement(469..654)
                     /locus_tag="ON345_003159"
     CDS             complement(469..654)
                     /locus_tag="ON345_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR3073182.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(651..3290)
                     /locus_tag="ON345_003160"
     CDS             complement(651..3290)
                     /locus_tag="ON345_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR3073183.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHTTATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MSDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEGNIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3498..4487
                     /gene="ldhA"
                     /locus_tag="ON345_003161"
     CDS             3498..4487
                     /gene="ldhA"
                     /locus_tag="ON345_003161"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR3073184.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4598..4990
                     /gene="hslJ"
                     /locus_tag="ON345_003162"
     CDS             4598..4990
                     /gene="hslJ"
                     /locus_tag="ON345_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR3073185.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQT"
     gene            complement(5017..5283)
                     /locus_tag="ON345_003163"
     CDS             complement(5017..5283)
                     /locus_tag="ON345_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR3073186.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5557..9081
                     /gene="nifJ"
                     /locus_tag="ON345_003164"
     CDS             5557..9081
                     /gene="nifJ"
                     /locus_tag="ON345_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR3073187.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADTWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRIRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2286 a         2406 c         2261 g         2251 t
ORIGIN      
        1 aaaacagata tttgactatg catttttcag ggcgaaaggc aatttgacag gagtttttga
       61 atattcacct tgtttcagga aaatagatca atcgctttct cctgtagcgt tatcccgata
      121 ttgcggtttt gctattaaaa ttttcgcacc catttaccgt ttatcccttg tacatattcc
      181 cccgatttgg cccgagctac gagcttttgc ccggcgagtt tcgcgatatc atctacagac
      241 acattatttt gctttgccag ttgttggtaa ctcgctttac gttcggcatt aatgtcggtt
      301 actaatttct ctgtttcagc atccgttttc agggcaacca gataaccata aaatgtttcc
      361 ccgacccgcc cctgcgttct ggcttcatcc agagtcaatg ccattacatt gctgcttacc
      421 agcccaacaa gaaacgcaca aagaagtaat gttttcttca tcatcgcctc agaaaagatc
      481 gctacgagtt tcaagcagct cttcgacatc tttgtctgcc ttgatgatga tctcatgctc
      541 aattttaacg ttcatattga tagtgatcgg ttccttgggc gcagcgactt caatacgcgg
      601 cgtacaggca gccagcataa aagatgacgt caacgcagcc agtaaaattt tcattgtttt
      661 tcctcacact cttttccttg cggacagtca tttcccggca gacgtgcgtt ctgctccaac
      721 catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat attcccttca
      781 tgatggtaat taaggtttac cgtaccgctt ttaccatcaa cgcgactggt acctgtaatg
      841 ttggcctgca tggttaataa accgagatta tctaaattaa tatttgtcga tgaacggcta
      901 atttccatat agcgcaacca gttaattgct gaacccgcag tcatattgtc tttcaccacc
      961 gcatctgcgg tgtctttatc cagccgcaag gtcattggcc cgctatttgc cagccaacca
     1021 tctttcacta tccatttttc gttgtttagc cataacggca atgcaccact aaatgccccg
     1081 gacatggcga attgtttcgg attgacggcg ctaaccagtt cgctggatga gagattattc
     1141 agccgcaaca gcgccgggtc atgttgcggc atacgtaatt gtttcatcag tacgttaccg
     1201 cctaacacat cgacgctaac atcagtcaac agcaagggtt cttcttcagt ccacggataa
     1261 cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac ttcggcaatt
     1321 cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa acggaaaggc
     1381 aggacaaaat cgacaccgtt aacctgatta tctgacatcc aggcactacc gcctttcaac
     1441 acgccgtgcc ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc aacctgagca
     1501 tacagttcac catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg ctggaataca
     1561 gtcagtgatt gttttggcca ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca
     1621 tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag gaaatacgtc
     1681 ggatcgcgcc catcgacgct aaattttaag gttgatggcg gtaacacact gccgccagtg
     1741 aacagcgttt gcccggcgtc cagtgacagc gcgccgctga aggagggatg ctgtgcgtca
     1801 cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt catcgtaccg
     1861 tattgtaact ggtcgaaacc ggtggagagg tcggtcagcg taatcgtgct gtcatgccat
     1921 tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg taaaactgcc ttttccccag
     1981 tagcgccact gccagcggcc cgcgtcaggg agaaaatcat tcgccagccc atccatatgc
     2041 agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa acgtccgtca
     2101 acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc gatgtccagc
     2161 gaatcgatga ctcttccctt cgaacgtaat aacgcgcctg gctcaaaggc cagcgttggg
     2221 tcagtcagac ttccacttag ctgcgcgggt aagcgggcat ataaaatgag gtccgcctgt
     2281 ttcgcttcac cggtcagctg cagaggcagc tggctgttat ccatgcttaa ttttcctggg
     2341 ccaaaattta gcaccgcgtt gcctttaccc gcttgcccct gggtcagcac actcagtcgt
     2401 ccgctgatca gagcgttctc aagccctgcc tgccagttgt cgactttgac acccagtcgg
     2461 ccactcaaag gaaaacctgc atacggccaa ctccagcgac catcgcttac ggtcaattgt
     2521 tgacgagtaa tttgccacgg caaatcgagc aacggatcgc cgttatcccg tgccagcaca
     2581 atcaattgcc cgctattttc ctgccagtcc agctctgcat ccaccagtga cggttcctgc
     2641 ggcaagttta acgtcgcagt agtatgccca cttacaggaa gtccatccgg cacgagcggc
     2701 atagtaaatt cccccaccag tttaaccggc ggctgatttt caaacgcgac gacatccagt
     2761 tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt ttcgccctga
     2821 taacgcagtt gctggatatc agaggttaat gcgagagaga gttttccctg ccattcctgc
     2881 cagggagaaa aaatcagttt atcgatattg atccaggtgt taggcagcat ggactgccac
     2941 tgcgcgaggg tttttggagc agctggcgat tgctccgtct gcggcaattt cgccagacaa
     3001 gcagaatcaa gttctaccgt gccgacgttc aataaccagc ggctgggatg tgaaaggctg
     3061 gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc gggaatgatt
     3121 aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc gagcggtagc
     3181 cagatgcccg ccagcgtggg aacccacagc ccgagcgtca tcaacagcgt caacggcaca
     3241 agaataatca gtaataacag cgcgagaacg gctttatatt tacccagcat gggtagttaa
     3301 tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg tttaaccgtt
     3361 cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa tatcgccata
     3421 gctttcaatt aaatttgaaa ttttgtaaaa tatttttagt agcttaaatg tgattcaaca
     3481 tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt
     3541 acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga
     3601 cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg
     3661 acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc
     3721 gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag
     3781 tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga
     3841 cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg
     3901 aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa
     3961 tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc
     4021 cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt
     4081 tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt
     4141 tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg
     4201 gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa attgggtcgt
     4261 tgggtatgga cgtctatgag aacgaacgcg atctattctt tgaagataaa tccaacgacg
     4321 tgatccagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc
     4381 accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact
     4441 taagcaatct ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct tgccgctccc
     4501 ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct ctattcttaa
     4561 aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaactag ccgcgtttgt
     4621 tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg ttacgccaga
     4681 acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga
     4741 taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa
     4801 ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag ggctggcaat
     4861 gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat
     4921 gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa
     4981 acagacatga acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc
     5041 ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt
     5101 accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc
     5161 actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac
     5221 aggttcacta ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg
     5281 catctcttct tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca
     5341 gagggcggcg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat
     5401 ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga
     5461 aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc gcagcaattt
     5521 cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc
     5581 gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc
     5641 agttccacga tggcagaaca ggctgatacc tgggccggaa acggcttgaa gaacgtttgg
     5701 ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc tactgtgcat
     5761 ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg
     5821 atgatcccga cgctgtacaa actggcaggc gaactcacac cgtttgtcct gcatgtagcg
     5881 gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga cgtcatggcg
     5941 gtgcgccaga cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc gcaagacttt
     6001 gctctcattt cgcacatcgc gacgctgaaa agccgcgtgc catttattca tttctttgat
     6061 ggtttccgca cgtcccacga aatcaacaaa attgtcccgc tggccgatga cacgattctt
     6121 gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcactcaa cccggaacat
     6181 ccggtgatcc gcggtacgtc cgccaatcct gacacttatt tccagtctcg cgaagccacc
     6241 aacccatggt acgacgcggt ctatgaccat gttgaacagg cgatgaatga tttctctgcc
     6301 gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc ggaacgggtg
     6361 attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc
     6421 cgtggcgaaa aagtcggcgt gctgaaagtt cgcctgtacc gccccttctc agccaaacac
     6481 ttgctacaaa ctctaccggg atccgtacgt agcgtggcgg tactggacag aaccaaagaa
     6541 cccggtgccc aggcagaacc gctctatctg gatgtgatga ccgcactggc agaagccttt
     6601 aataatggca agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg tctttcatcc
     6661 aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg
     6721 aaagcgcgct ttacggttgg tatttacgat gatgtaacca atctgtccct gccgttgccg
     6781 gaaaacaccc agccaaactc ggcgaaactt gaagccttgt tttatggcct tggtagtgat
     6841 ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac
     6901 gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt
     6961 cgagtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc
     7021 tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc
     7081 attttcctgc tcaacacacc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaagaa
     7141 gttcaggccg tgttaaacca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc
     7201 gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat
     7261 ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa
     7321 agttacagta gcaaaggcca ggatctggtg gaacgcaatt ggcaggctct ggcgctggcg
     7381 cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg
     7441 ccagtggttt ccgatgccgc tcctgatttc gtgaaaaccg taaccgctgc gatgctcgcc
     7501 gggcttggtg acgccctccc cgtttcggcg ctgccgccag acggctcctg gccgatgggc
     7561 actacgcgct gggaaaaacg taatattgcc gaagagatcc ccatctggaa agaggaactc
     7621 tgtacccaat gtaaccactg cgttgccgct tgcccacact cagctattcg cgcaaaagtg
     7681 gtgccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg
     7741 cgtgatatgc gcgggcagaa atatgtcttg caggtggcac cggaagattg caccggttgt
     7801 aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc
     7861 aatatgatgt ctcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc
     7921 aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg
     7981 attacaccgc tgtttgaata ttcaggtgct tgctccggtt gtggcgagac gccgtatatt
     8041 aaattactga ctcagctcta tggcgaccgg atgttgatcg ctaacgccac tggctgttct
     8101 tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa cggtcgtggg
     8161 ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg
     8221 acggtcgatc aacaccgtat ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc
     8281 ccggcggaat tactgacggc gttgaaatca gacgccacgc cagaggttcg tcgtgaacag
     8341 gttgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact gctacgtgat
     8401 gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gtgatggctg ggcttacgat
     8461 atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgttaa cattctggtg
     8521 ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac accgctgggt
     8581 gcagtaacta aatttggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt
     8641 atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca gctgaaccag
     8701 acggtgaaag cgattcagga agcggaagcg tatccggggc catcgctgat cattgcttat
     8761 agcccgtgtg aagagcatgg ttacgatctg gcactcagcc acgaccagat gcgccaactc
     8821 actgccaccg gcttctggcc gctatatcgc tttgatccgc gtcgtgccga tgaaggcaaa
     8881 ctgccgctgg cactggattc acgcccgccg tcagaagcac tggaagaaac gttacttcat
     8941 gagcagcgtt tccgtcggct gaattcgcag cagccagaag tggcagaaca gttatggaaa
     9001 gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg
     9061 gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaagcc
     9121 cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc
     9181 caacagaaaa taaaatctgt gaca
//
LOCUS       DAJSKJ010000157         9002 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000157 DAJSKJ010000000
VERSION     DAJSKJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.159"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..192)
                     /locus_tag="ON345_003165"
     CDS             complement(<1..192)
                     /locus_tag="ON345_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073188.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQYG
                     EFFSLNYKLTKRIYFITFIY"
     gene            270..1364
                     /gene="ddlA"
                     /locus_tag="ON345_003166"
     CDS             270..1364
                     /gene="ddlA"
                     /locus_tag="ON345_003166"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3073189.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1388..1600)
                     /locus_tag="ON345_003167"
     CDS             complement(1388..1600)
                     /locus_tag="ON345_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR3073190.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1860..2168
                     /locus_tag="ON345_003168"
     CDS             1860..2168
                     /locus_tag="ON345_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR3073191.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2227..3321)
                     /gene="yaiW"
                     /locus_tag="ON345_003169"
     CDS             complement(2227..3321)
                     /gene="yaiW"
                     /locus_tag="ON345_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR3073192.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3334..4554)
                     /gene="sbmA"
                     /locus_tag="ON345_003170"
     CDS             complement(3334..4554)
                     /gene="sbmA"
                     /locus_tag="ON345_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR3073193.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4906..6063
                     /gene="ampH"
                     /locus_tag="ON345_003171"
     CDS             4906..6063
                     /gene="ampH"
                     /locus_tag="ON345_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR3073194.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSRNKPLVIPAS"
     gene            complement(6064..6687)
                     /gene="iprA"
                     /locus_tag="ON345_003172"
     CDS             complement(6064..6687)
                     /gene="iprA"
                     /locus_tag="ON345_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR3073195.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6775..>9002)
                     /locus_tag="ON345_003173"
     CDS             complement(6775..>9002)
                     /locus_tag="ON345_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR3073196.1"
                     /translation="YHQWDNNINVSNSTVTSGSQTPLEESDGFFGKSAEPSDYAGNGG
                     QNDVALSFTDNAGSNYSMKNNVNFDHSTLLGDVEFTSHWNNDGVFFYSTGHDSNGDGV
                     LDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAEVIAPADMYDVAPNSLTPGATIE
                     ANDWGRIIDNKVFQSGVFNVALNNSSEWNTVNSSVIDTLAVNNGSQVNVTDSSLVSDT
                     IGLTNGSSLNIGANGVVATDHLTVDSYSTVNLTESTGWNNYSNLYTNTITVTNGGVLD
                     VNVDQFDTEAFRTDKLELTSGNIADHNGNVISGVFDINSSDYVLNADLVNDRTWDTSK
                     SNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVRIDNATGAGSI
                     ADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALS
                     IPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGI
                     MVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDG
                     SLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLF
                     QSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNND
                     NDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYT
                     W"
BASE COUNT         2298 a         2265 c         2150 g         2289 t
ORIGIN      
        1 ataaataaaa gttatgaaat atattctctt cgttaactta taattaagag aaaaaaactc
       61 accgtattgc catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt
      121 gcgcacatct ttgagataac gcactttctt caccagtacg ttgcacgctt tgtcagcaga
      181 agattgcaac aatctttcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca
      241 ctttgtgtta gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg
      301 gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca
      361 ttgataaaag tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg
      421 tcagcgatgc cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc
      481 cttcggcgac cagccttgcg caggtgccag gtaaacatga gcatcagctt atcgacgcgc
      541 aaaacggtca gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg
      601 gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg
      661 atgttctggc ttcagcagcc tgtatggata aagatgttac caaacgtctg ctgcgcgatg
      721 ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc caatcgtcac aacattagtt
      781 ttgccgaagt ggagtctaaa ctggggttac cactgtttgt aaaaccggct aatcagggct
      841 cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc gtcgatctgg
      901 cgttcgaatt cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat
      961 gcgcagttct gggcaacgac aacccgcaag ccagcacctg tggcgagatc gtactcacca
     1021 gcgacttcta tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc
     1081 cggcagccat tgcgccagaa atcaacgata agatccgggc gattgccgtt caggcttatc
     1141 aaacgttggg atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag
     1201 tggtgatcaa cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc
     1261 tgtggcaagc cagcggtctc ggttacaccg atctgatcac acgtttgatt gaactggcgc
     1321 tggagcgtca cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat
     1381 aatattctta ttgggcgttt tcagtttctt catcgcgacg acgaataatc agccccgcca
     1441 gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt
     1501 caaatcccag tagccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat
     1561 agtagtgcca gtcacggcgg atttttacag gcagattcat ggctgctccg ttaacgaaaa
     1621 acaacatcct tgcatattct gcaacaaccg tctgtggtgt agacaacact ttcgcaattg
     1681 ttaataaatt taacttaaag ttgctgtttt gtgactcgca acgaagattc acgcttcggg
     1741 aatattccca acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga
     1801 cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta
     1861 tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa
     1921 cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat
     1981 tgctgaacct gttagtgcat gcgcccggcg tctatgagcg tttaatgcaa gtccaggaaa
     2041 caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt
     2101 ttttagtagg ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc
     2161 gccagtaagt tttacgttgt cagatgcggc ctgaaacgcc ttatctggca tgacattcct
     2221 gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc
     2281 cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg
     2341 ggggagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt
     2401 ctcctcaaaa gagaagctgt cacctttctc taactgacgg cgaatttcgc tgtcgctcat
     2461 ccccagtttt gccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt ctttactgtc
     2521 gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgaccgcgtt
     2581 ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa agcggtatat
     2641 cggtgcgcta tagctggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc
     2701 gcggcgactg aaaacttcct gacgaactgt accgtccatt ttccacggat accctttggt
     2761 atgctgttcg gcaaaagcaa tgctgacctg catcggccca ccggtgcgca caggattaag
     2821 cgaaccaaac agcgtctgcc ccattggcac catgctgatc agatcgtcaa aaatcgcgct
     2881 caattgcttt tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga
     2941 tttgattttt agcgcggtat gaaccagaaa agcaggaatg tgcatccgtt cagcacgacg
     3001 gtcaatctct tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt
     3061 tgactcctgc tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg gtgccagccc
     3121 ctgactttca aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc
     3181 aggcatcttc tgccgcacga cgctcgccac atctatcgct ttttcgccct ttttcaacgg
     3241 ttgcggagcc tgactactac accccgctaa aaccagaacc gccaacaatg acagcgaaga
     3301 tagatgattg acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg
     3361 aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa
     3421 ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa
     3481 aacgttggta atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa
     3541 cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatgttgaa
     3601 atacatatag tgaaaataga ggcgaaaata gtttttccgt acggcgctaa acagctcgcg
     3661 taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta
     3721 ggcggcctct actcgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc
     3781 cagcaatccg gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat
     3841 gtgcccgata atcggcagct ccggcacatg cgcggagagc gttaccagca ccggcaggaa
     3901 ggcgatcaac gtcatgatgg cgttgataaa actgacgccc atattctcca gcgttgaagc
     3961 aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct tcgatatgac gcagttgttg
     4021 ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac
     4081 aaagaagttg ttcagtacac tgatcaccac cgcgatcagc gcaatcccca gaaagacgcc
     4141 cacttcgcgg taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat
     4201 cagatcatag aacggcgcat accaggcgtt gacggcgacc ccgacttcca ccaaaaacca
     4261 ggtgacgaag ataatcagcg cagtaccgag tattgaccag tattgccaac ggtgcgggct
     4321 gtagataaac cagaacaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat
     4381 caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt
     4441 gatacgcgcc acccagtcac ccccaccggc ttgccagaag ataacggcaa tcaatgccca
     4501 aacaaaggcc gagagaaaaa atgctcccgg ctttgggaaa aaagacttaa acatatgcac
     4561 tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccactataaa
     4621 gcgtcagcca aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct
     4681 gaccataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta
     4741 acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc
     4801 cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag
     4861 tataaatacg ctcagttacc ttcattcaat ttatggacac caccgttgaa acgtagtctg
     4921 cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc
     4981 actgaaccgg agttcgcctc tgatattgtt gatcgttatg ccgatcatat tttttacggc
     5041 agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgcagt
     5101 tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt
     5161 gcttccctca ccaagctaat gaccagtgag atgctggtga aattgctcga ccaggggacc
     5221 gtgaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt gccaacctac
     5281 aatgggacgc ccattacgct ggtcaatctg gcaacccata ccagcgcgct gccccgtgaa
     5341 cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg
     5401 aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac
     5461 cttgcgttcg acctgctggc tgatgcactg gcgaatgcgt ccggcaagcc ttatacccag
     5521 ttgtttgaag agcagattac ccgtccattg gggatgaaag acaccaccta caccccctca
     5581 ccggatcagt gccgccgttt aatggttgca gagcgcggtg ccagtccatg caataacacg
     5641 ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg
     5701 cagcagtatt tgtcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg
     5761 ttgatttatc agcgcgcgca gtttacgaaa gtgattggca tggatgtccc cggcaaagcg
     5821 gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc ggggattatt
     5881 cagaagacag gaggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat
     5941 attggcgcgt ttgtggttgt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat
     6001 ggcatcaatg acctggtgac cgagctaagc aggaataaac cgctggttat ccccgcgtcc
     6061 tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat
     6121 atagccgcct ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt
     6181 acgttgatgg atataattca tcacaccaat acgtgagcgc aattcttcat tccagtcaat
     6241 cattgataat aatgttgcgc ggatttgttc gtaggaatta tgcccaatga gctgcacgtc
     6301 gcgtaattcc agaattctat tttgccaggc taaccagtaa aaagcgtctc gccagagttg
     6361 attttgttca ataagcgtaa tggtttgttt ggctggtaga tgatatcccg tacaatttcc
     6421 ttctgatatt aatttgtatg gtatatcgtt tttcattaaa ccatcagcca gccccataat
     6481 ataaggagcc tgggtaatac cgataagtac gttttcttct ctacgcagag agataacgcc
     6541 ctctaaaata acgaaagtat cttcgctatt gacatcactg gaaaatataa cttgcttttc
     6601 attattaaac tcgaagcgcg taccgtatct ggacaaacat ttatcgagct taccaaattc
     6661 ctgaagaggt ttaactacag ataacatttg cgcgtccttt gcagtaatgc ccgtcaaatc
     6721 cttgacgggc attatttaga ttaaattacc agaatttctt cggagtgaag aatattacca
     6781 ggtatattta acacccacgt tcgcggacca gtcttgatct acgtcaccac caccgaggta
     6841 gttagcatcg gtataggcgc tgaagttctt ggtgaagctg aactgagtac ccagaccaac
     6901 acgtaccgca gacccttcag taccgttatc gatggaatca ccgttcacat cgttatcgtt
     6961 gttagagtcg tcgtagacgt aagccagttt gaagtacgga gtcagagcct ggtcttcgct
     7021 gtaggtgaag gtataacctg catctacacc cagttcataa cgcatgctgt cgtaagactg
     7081 accgtcaact ttcatgtcgt tgctcagctg gtagtcatca ccagactgga acagaccaga
     7141 aacgctgccg taaggagtca cgtaaccagc atcacccagt ttgaagtcgt aaccggcttt
     7201 caaaccgaag ccccaagcgt cggagttggt gctaccgtca acgtaagtac cgttgctcat
     7261 ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag ctaccatcaa caaagacgtt
     7321 gttcgcgaag tgagcagaag agtagatgta ggcagtctgg ctgtcttgat ccacctgacc
     7381 agaacggtca ttcatgtcac ctttagcgaa gcctgcagcc gcaccgacga tccacttagc
     7441 gttgttaccg tcaattttgg tatcaacacc gaccatgatg ccgttaacat cctgatcata
     7501 gttgatggtg ccgttgtcgc cgttgaagtt accaccgaag tagcttaccc atgcgccgcc
     7561 gttatcagcc aggccatgac gagagttggt cagacgagta ccaacggtgt cttgttccag
     7621 gttccagata ttggtgttcg cagacgggat gctcagcgcc atgttagcgt agtcggtcag
     7681 ctccatctgt tgcagaacaa cggtgttacc gcgctgttca gcctgatagg tgtatgcacc
     7741 caggtcagct ttgttagcag cagagaaggt cgcgttgctg tttttgtcgt tgacgtagat
     7801 cagctcgttg ccgttgtagt cagcgataga accagcacca gttgcgttgt cgatacgaac
     7861 tttgtagtta ccggttgcag caacaacgtt atcaacagag ctgttatcca gttcagtacc
     7921 gttgtctacg tcgccgttac cattgatagt caggtgacca tcagagttca tagcaacaat
     7981 accgtaaccg tagttagact tggaagtatc ccacgtacgg tcgttcacca gatcagcgtt
     8041 cagaacgtaa tcgctgctgt tgatgtcgaa cacaccagaa attacgttgc cgttatggtc
     8101 agcaatgttg ccgctggtca gttccagttt atcagtacgg aaagcttcag tatcgaactg
     8161 atcaacattc acatccagca caccaccgtt agtcacagtg atagtattgg tatacagatt
     8221 agaataatta ttccaaccgg tagattcggt taaattaacg gtactgtagc tatcaacagt
     8281 caggtggtct gtagcaacca cgccatttgc accaatattc agtgaagaac cgttggttaa
     8341 accaatggta tctgatacca gagaggaatc agtaacattt acctgagagc cattatttac
     8401 agctaatgta tcaattactg aactattaac ggtattccat tctgaactat tgttcagtgc
     8461 tacgttaaat acgccgctct ggaatacttt gttgtcaata atacggcccc aatcattagc
     8521 ttcgatggtg gcaccagggg tcaggctgtt cggcgcaacg tcatacatat ctgccggagc
     8581 aattacttca gcactcatgt ttgctgaacc aacccactta ctaccgttat tcagagtaat
     8641 attcagttcg tcaacgttct gagcgtcatc tacccaaccg ccgtttgtat caaggacgcc
     8701 gtcgccgttg ctgtcatgac cggtggaata gaagaatacg ccatcgttat tccagtgact
     8761 tgtaaattcg acatcaccca acagagttga atggtcaaag tttacattgt ttttcatgga
     8821 ataatttgag ccagcattgt cagtgaatga cagtgctacg tcattctggc caccattacc
     8881 agcgtaatca cttggttcag cagatttacc gaagaagcca tcactttctt ccagcggagt
     8941 ctgtgatcct gaagttacag tggaattaga cacattgatg ttgttgtccc actgatgata
     9001 tt
//
LOCUS       DAJSKJ010000158         8881 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000158 DAJSKJ010000000
VERSION     DAJSKJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.160"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..606)
                     /gene="chiP"
                     /locus_tag="ON345_003174"
     CDS             complement(<1..606)
                     /gene="chiP"
                     /locus_tag="ON345_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR3073197.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPW"
     gene            complement(1183..2847)
                     /gene="glnS"
                     /locus_tag="ON345_003175"
     CDS             complement(1183..2847)
                     /gene="glnS"
                     /locus_tag="ON345_003175"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR3073198.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVNAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(3051..4997)
                     /gene="nagE"
                     /locus_tag="ON345_003176"
     CDS             complement(3051..4997)
                     /gene="nagE"
                     /locus_tag="ON345_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR3073199.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            5330..6130
                     /gene="nagB"
                     /locus_tag="ON345_003177"
     CDS             5330..6130
                     /gene="nagB"
                     /locus_tag="ON345_003177"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR3073200.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVSLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            6190..7338
                     /gene="nagA"
                     /locus_tag="ON345_003178"
     CDS             6190..7338
                     /gene="nagA"
                     /locus_tag="ON345_003178"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR3073201.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     KNADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            7347..8567
                     /gene="nagC"
                     /locus_tag="ON345_003179"
                     /gene_synonym="nagR"
     CDS             7347..8567
                     /gene="nagC"
                     /locus_tag="ON345_003179"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR3073202.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNIGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIQSCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            8615..>8881
                     /gene="nagD"
                     /locus_tag="ON345_003180"
     CDS             8615..>8881
                     /gene="nagD"
                     /locus_tag="ON345_003180"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR3073203.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYV"
BASE COUNT         2214 a         2295 c         2234 g         2138 t
ORIGIN      
        1 ccatggtgct ttgtattcat tggtccacat gtaggagaaa ctcaacgcac cagcatcgcc
       61 gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta cctggcataa agctccagtg
      121 cggcgctaac agcgtttgac cagttggctg aatgtaacct gccctcgccc aaaccggacc
      181 gtatttaaat ttggccgcag ctttatacag gcttataccg cttttgtcgc cggaccagtc
      241 ttcgtcatag gctttattac tttttgaaaa cgcgatttcg ttcgggtggg agctgtcgcc
      301 gttttccgcc atttcaatcg ccgtaaacgc agcaatatca aggccgaaca tatcagcagc
      361 ataaccggac tgaaaatcga ggttggcatt ccaggtggag tgagaaaggt tggttttgta
      421 tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc tgccagtaat atataccgcc
      481 ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc tccggcattg ccataaagcc
      541 cgacattgct gtaacaccgg cgatagccag cgccagcgta ctacgtttgc cactaaacgt
      601 acgcatgggt taatcctctt tgacgtataa attgctgcac caaaggtgaa cagcgaaaaa
      661 ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga ctacaggaac agactatttt
      721 tcgcgacgcg aattatcaat ctctttctgt aaccagaatg taagaacatg agcaaccgca
      781 catattttga tgatttttgt tacaaggctt aagtttgcgg ttgaaaggct tgcagaagaa
      841 taaaagcgtg aaattccagc aagatgaaag cggttacatt ttatttaatt atgttttgtt
      901 attttaaatt tattttatgg ataaattgcg aagcggaatt aattcgcgaa gaaaagcaga
      961 aaaaacgccg ctgaacgcgg cgttttggat tgtttgcata tttcttgccg gatgcggcgt
     1021 gaacgcctta accggcctac aaaagcacgc aaactcaata tattgcagag atcatgtagg
     1081 cctgataagc gtagtgcatc aggcaattta gcgtttgaaa ctgttgtctg ttccttgccg
     1141 gatgcgacgt aaacgcccca tccggcataa cgaaacttaa aattactcgc ctactttcgc
     1201 ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg tagaatggcg
     1261 gctatcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg caaccgcatc
     1321 tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt taatcaccga
     1381 caggaaatca tccgcagcac cagggttcgg cacgctgaac agacggtcgt acagacggat
     1441 ttcaaccggc agcgcatgtg ccgcgttcac ccagtgaata acacctttga ctttacgacc
     1501 atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg tggtgatatt
     1561 gccttcggca tctttctcca cgcgttctgc cttaatgacg taagcattac gcagacgcac
     1621 ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga aatcggcgcg
     1681 atcgatccaa atctcaccgc taaacggcac ctggcggcta cccatttccg gtttgttcgg
     1741 atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa ccagtttcac
     1801 cggatcgata accgccattg cgcgcggggc gttttcgttg agatcttcac ggatgcagga
     1861 ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa
     1921 ctcacgaata gaagcagcag tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg
     1981 cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga
     2041 catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat
     2101 ggtgatgttg tccagcaccc agtcgtacag gcgacggttg tcctggaact caagcgtaca
     2161 cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt cgtacatcgg
     2221 gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc ggtacagcac
     2281 cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca ggcaggcttt
     2341 accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaggttct cttcaacgct
     2401 gcggtcgcgg tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt attcgcggat
     2461 ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata
     2521 ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc
     2581 taaccactct acgtcgtttt tgatcgactc aacatactcg atatcttctt ttaccgggtt
     2641 agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag
     2701 gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt
     2761 gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac ggataaagtt
     2821 agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac
     2881 ggcgtatgat cttataagcg ggacaggctg acaactgtta gttttttaaa attgataatc
     2941 taacaaggat tatggggatg attgatgaaa aaaaaaagcg gcagagaaga tctccaccgc
     3001 ttgtcattgt tggatgcgac gctcaagcgt cgcatcaggc ataaagcaga ttactttttg
     3061 atttcataca gcggtgtttg acccgccaca acatggccct gagctttaat gatcaagcca
     3121 ctgaaatcgt cgatattgct gcaaaccacc gggctaatca tcgagcgggc gttagcgttc
     3181 aggtaatcca gatccatttc cagaatcggt tgccctgcgc ttacctgcgc accctcttcc
     3241 accagacgtt taaagccttt accttccagc gctacggtgt cgatacccat atggacgacg
     3301 atctccgcgc ctttttcggt ttccaggcag aacgcgtggt tggtgttgaa gattttcacg
     3361 attgtccctg cggctggtga tacgacgatt ttatctgtcg gtttcaccgc cacaccgtca
     3421 cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac gacatcaccg
     3481 gtaatcggcg ataccagctc cgcgatagat accgcgtttg gtacagcctg cggctttgct
     3541 acaggcgcgg cagttgccgg agttgcttca gctgacgcag cggctaccgg accacgggca
     3601 acgactttct tcatcgcatc gccgatggat tctgctttcg cgccaacaat cacctgaata
     3661 gtctgtttgt tcagtttcac tacaccagaa gcacccagac gtttacacat cgtatcgttg
     3721 acgcgggcag agtcagccac ggtcaggcgc agacgggtga tacaggcgtc aattgctttc
     3781 aggttgtcag tgccgccaac cgcagcaata tagttggtcg ccagttgatt gaggccttct
     3841 tcagtgttgc tgttggcttc ttcagtaacg atctcgtctt ctttatcttc acgacccggc
     3901 gttttcaggt tgaacatgcg gataaccaaa ctgaacacca cgaagtagat agcgaagaag
     3961 acaacaccca tcaccagcag catccagacg ttctggctgg cggccggcag gttatacatc
     4021 aacgcgtagt cgatagcccc cgcagagaaa gagaagccag catggatacc cagcagtgtt
     4081 gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag cggtgcaagg
     4141 aacatgaaca ggaattccag cggctcagtc acaccggtca ggaacgcagt aacagcaaca
     4201 gaaagcagca tcccgccaac catcggacga cgctctttcg gtgctgcgaa gtacatcgcc
     4261 agcgccgcac ccggcagacc gaacatcata atcgggaaga agccggacat gaacatcccc
     4321 gcggtgccgt caccggcata gaagcggttg atatcaccgt ggaaaaccgt acccgccgcg
     4381 ttggtgaatt caccaatctg gaaccaggcg atggtgttca gcacctgatg cagaccggtt
     4441 gggatcagca gacggttgat gaaaccaaag ataccggaac ccagcgcgcc cgcagaaacg
     4501 atccactcgc ctcctgcatg gatagcgtgc tgtaccggcg gccagacgta accaaaaatg
     4561 gccgccagca ccaggcagaa gaagccggtg gcaatcggca caaagcgttt gccgccgaag
     4621 aagctcagga agtccggcag tttaatatcg gaccaacggt tataggctgc gccaccaacc
     4681 agaccggtaa tgatacccgc cagtacaccc atgtttattt ctgggttgat ggtcaccatc
     4741 gctttggtta acacaaagta acctaccgca cccgccagcg ccgccgcacc tgcgctgtct
     4801 ttcgaccagc tggatgccac accgatggcg aagattaatg cgaggttatc aaaaatcgca
     4861 ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa tcgcagcaac
     4921 agtgccgcca ccggcagcac cgcgataggg agctgtaacg ccctaccgag tcgctggaaa
     4981 aaacctaaaa tattcatctt attcccccta cgagaaccct atttggctcg tttaaagccg
     5041 tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt aatcagattg ttagatcatc
     5101 tgctacagag tgtgtgaaaa tttaattcgt atcgcaaatt aaacgcgtgt cttttgtgag
     5161 ttttgtcacc aaatatcgtt atcatcactc ccttttactg gctaaaccag aaaacttatt
     5221 ttatcattca aaaaatcagg tcggattgac gcctgtctgc gcaaatccag gttacgctta
     5281 aagatgccta atccgccaat ggcttacatt ttacttattg aggtgaataa tgagactgat
     5341 ccccctgact accgctgaac aggtcggcaa atgggctgct cgccatatcg tcaatcgtat
     5401 caatgcgttc aaaccgactg ccgatcgtcc gtttgtactg ggcctgccga ctggcggcac
     5461 gccgatgacc acctataaag cgttagtcga aatgcataaa gcaggccagg tcagctttaa
     5521 gcacgttgtc accttcaaca tggacgaata tgtcagtctg ccgaaagagc atccggaaag
     5581 ctactacagc tttatgcacc gtaatttctt cgatcacgtt gatattccag cagaaaacat
     5641 caaccttctc aacggcaatg ccccggatat cgacgccgag tgccgccagt atgaagaaaa
     5701 aatccgttct tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg acggtcatat
     5761 tgcatttaac gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa ccctgactca
     5821 tgacactcgc gtcgcgaact ctcgtttttt tgataacgat gtcaatcagg tgccaaaata
     5881 tgccctgact gtcggtgttg gtacattgct ggatgccgaa gaagtgatga ttctggtgct
     5941 gggtagccag aaagcactgg cactgcaggc tgcagttgaa ggttgcgtga accacatgtg
     6001 gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg gtgtgcgatg aaccttccac
     6061 catggagctg aaagttaaga ctttaagata tttcaatgaa ttagaagcag aaaatatcaa
     6121 aggtctgtaa ttgttatccc tgccctggct ccttgctcag ggcaatattt tttaaaatcg
     6181 ggggtcagaa tgtatgcatt aacccagggc cggatcttta ccggccacga atttcttgat
     6241 gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg tctgtccggt agcggaactg
     6301 ccgccagaga tcgaacaacg ttcactgaac ggggccattc tctcccccgg ttttatcgat
     6361 gtgcagttaa acggctgcgg cggcgtacag tttaacgaca ccgctgaagc ggtcagcgtg
     6421 gaaacgctgg aaatcatgca gaaagccaat gagaaatcag gctgtactaa ctatctgccg
     6481 acgcttatca ccaccagcga tgagctgatg aaacagggcg tgcgcgttat gcgcgagtac
     6541 ctggcaagac atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg gctgaatctg
     6601 gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc gctggtcgat
     6661 ttcctgtgta agaacgccga cgtcattacc aaagtgaccc tggcaccgga aatggttcct
     6721 gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg tcactccaac
     6781 gcgacgttga aagaagcgaa agccggtttc cgcgcgggga ttacctttgc cacccatctg
     6841 tacaacgcga tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc gatcctcgac
     6901 gaagctgaca tttattgcgg tattatcgct gatggcctgc atgttgatta cgccaacatt
     6961 cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc caccgcgcca
     7021 gcaggtgcca acattgaaca gttcattttt gcgggtaaaa caatatacta ccgtaacgga
     7081 ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct taaccatgat tgaaggcgtg
     7141 cgtaatctgg tcgaacattg cggtatcgca ctggatgaag tgctgcgtat ggcgacgctc
     7201 tatccggcgc gtgcgattgg cgttgaaaaa cgtctcggca cgctcgccgc aggtaaagta
     7261 gccaacctga ccgcattcac acctgatttt aaaatcacca agaccatcgt taacggtaac
     7321 gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg acaagctcag ataggtaatg
     7381 ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg cctgattgac cagtacgggc
     7441 caatctcgcg gattcagatt gccgagcaaa gccagcttgc ccccgccagc gtaaccaaaa
     7501 ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag gcctccaccg
     7561 ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa tttccacgca atcggcgtac
     7621 ggcttggtcg tcacgacgcc accatcaccc tgtttgatct cagcagcaaa gtgctggcag
     7681 aagaacatta cccgctgccg gaacgtaccc agcaaacgct ggaacatgcc ctgttgaatg
     7741 ccattgctca gtttattgat agctaccagc gcaaactgcg cgagctgatc gcgatttcgg
     7801 tgatcctgcc agggcttgtt gacccggaca gcggcaaaat tcattacatg ccgcatattc
     7861 aggtagaaaa ctgggggctg gtagaagctc tggaagaacg ttttaaagtg acctgtttcg
     7921 ttggtcacga tatccgtagt ctggcgctgg cggagcacta cttcggtgca agtcaggatt
     7981 gcgaagactc cattctggtg cgtgtccatc gcggaaccgg ggccgggatt atctctaacg
     8041 ggcgcatttt tattggccgc aacggtaaca tcggtgaaat tggccatatt caggtcgaac
     8101 cgctgggtga acgctgccac tgcggcaact ttggctgcct ggaaactatc gccgccaacg
     8161 ctgccattga acaacgggtg ttgaatctgt taaagcaggg ctaccagagc cgcgtgccgc
     8221 tggacgactg caccatcaaa actatctgca aagccgcgaa caaaggcgat agtctggcgt
     8281 cggaagtaat tgagtatgtc ggtcgtcatc tgggtaaaac catcgccatt gctatcaact
     8341 tatttaatcc gcaaaaaatt gttattgccg gtgaaatcac cgaagccgat aaagtgctgc
     8401 tccctgctat tcaaagctgc attaataccc aggcgctgaa ggcgtttcgt actaatctgc
     8461 cggtggtacg ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg ctggtaaaac
     8521 gcgccatgct caacggtatt ttgctccagc atttgctgga aaattaatgt gcttttatag
     8581 tggcgcttat tgttgtcaat attctgggta gtccatgacc attaaaaatg taatttgcga
     8641 tatcgacggc gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg aatttttgca
     8701 cgggattatg gataaaggcc tgccgctggt gttgctgacc aactatcctt cgcagaccgg
     8761 gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat gtgccggata gcgtgtttta
     8821 tacctctgcg atggcgaccg ccgatttcct gcgtcgccag gaaggcaaga aagcatatgt
     8881 g
//
LOCUS       DAJSKJ010000159         8741 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000159 DAJSKJ010000000
VERSION     DAJSKJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.161"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            126..449
                     /locus_tag="ON345_003181"
     CDS             126..449
                     /locus_tag="ON345_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR3073204.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            620..1117
                     /gene="ftnA"
                     /locus_tag="ON345_003182"
     CDS             620..1117
                     /gene="ftnA"
                     /locus_tag="ON345_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR3073205.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(1155..1394)
                     /locus_tag="ON345_003183"
     CDS             complement(1155..1394)
                     /locus_tag="ON345_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR3073206.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1585..2796
                     /gene="tyrP"
                     /locus_tag="ON345_003184"
     CDS             1585..2796
                     /gene="tyrP"
                     /locus_tag="ON345_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR3073207.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2858..3523)
                     /gene="yecA"
                     /locus_tag="ON345_003185"
     CDS             complement(2858..3523)
                     /gene="yecA"
                     /locus_tag="ON345_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR3073208.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3719..3805)
                     /locus_tag="ON345_003186"
     tRNA            complement(3719..3805)
                     /locus_tag="ON345_003186"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3769..3771),aa:Leu,seq:taa)
     gene            complement(3818..3891)
                     /locus_tag="ON345_003187"
     tRNA            complement(3818..3891)
                     /locus_tag="ON345_003187"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3857..3859),aa:Cys,seq:gca)
     gene            complement(3946..4021)
                     /locus_tag="ON345_003188"
     tRNA            complement(3946..4021)
                     /locus_tag="ON345_003188"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3986..3988),aa:Gly,seq:gcc)
     gene            complement(4173..4721)
                     /gene="pgsA"
                     /locus_tag="ON345_003189"
     CDS             complement(4173..4721)
                     /gene="pgsA"
                     /locus_tag="ON345_003189"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR3073209.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4778..6610)
                     /gene="uvrC"
                     /locus_tag="ON345_003190"
     CDS             complement(4778..6610)
                     /gene="uvrC"
                     /locus_tag="ON345_003190"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR3073210.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6607..7263)
                     /gene="uvrY"
                     /locus_tag="ON345_003191"
     CDS             complement(6607..7263)
                     /gene="uvrY"
                     /locus_tag="ON345_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR3073211.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7559..7735
                     /locus_tag="ON345_003192"
     CDS             7559..7735
                     /locus_tag="ON345_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073212.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7722..7946
                     /locus_tag="ON345_003193"
     CDS             7722..7946
                     /locus_tag="ON345_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR3073213.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(8014..8736)
                     /gene="sdiA"
                     /locus_tag="ON345_003194"
     CDS             complement(8014..8736)
                     /gene="sdiA"
                     /locus_tag="ON345_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR3073214.1"
                     /translation="MQDKDFFSWRRTMLLRFQRMEAAEEVYHEIEFQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2162 a         2230 c         2031 g         2318 t
ORIGIN      
        1 acagcccccc tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt
       61 tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct
      121 ggaccatgag acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta
      181 ctcgccaagc ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg
      241 accaggcttt tttgcagcat gcccataccg atcgttatgt cagtcgcggg atcgccgatc
      301 gcgcatgcca gcaggaaggc tatacccacg cggtcccctt tggtcagcca gtaggcaact
      361 gtagcctttt tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc
      421 atcattccgc cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga
      481 ttaaagaaat agcggtaatg cgttttattc ccattcgtat ttttaataat tgaagtttat
      541 attttacctt ttgcaaataa taaaataaca aattatagtg acgccacagc aacaaatata
      601 acctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat
      661 gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca
      721 taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat
      781 gcagcgtctg tttgattacc tgactgatac cggcaattta ccgcgtatta ataccgttga
      841 atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga
      901 acaattaatc acccagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta
      961 cccaacattt aatttcctgc agtggtatgt ttctgagcag catgaagaag agaaactgtt
     1021 caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc tgtattttat
     1081 cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctccggcgg caggtttact
     1141 gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accgtctttc
     1201 gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc tgtcatccct
     1261 tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc
     1321 agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcatgaccg
     1381 tgaatagagt ccatcgtccc tcctcaaaaa aagcctagcg tagcgattgc cgcttatgaa
     1441 gactttgcgc cagcgcagga ctgaatgctt tttattgtac atttatattt acaccatatg
     1501 taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat atcactcata
     1561 aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt ttttatcgtg
     1621 gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc cggtgttggt
     1681 tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta
     1741 ttactgctgg aggtgtacca gcatgttccg gcagataccg gtctgggcac gctggcaaaa
     1801 cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt cttaatgtat
     1861 gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac
     1921 tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt
     1981 ggcgttgttt gtgttggaac atcgctggtc gatttattta accgttttct gttcagcgcc
     2041 aaaattattt ttctggtggt gatgctggta ttgctgctac cgcatattca caaagtgaat
     2101 cttttaaccc tgccgttgca acaggggctg gctctgtcag caatcccggt gatttttacc
     2161 tcatttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg caacgttcgt
     2221 aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata tattttctgg
     2281 caggtggcaa cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat
     2341 gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag
     2401 ctggcagtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg cgttgcgtta
     2461 ggcttatttg attatctggc tgatttattt cagcgttcaa ataccgttgg tggacggttg
     2521 caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta tccacgagga
     2581 tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct
     2641 tcgctgttga cctggcaaag cagaaagcac aatcctcagg cgggttaccg ggtgaaaggt
     2701 ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt
     2761 ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa attccttatt
     2821 gggtgccaga attaacgctg acacccaatt tgacctctta atgcaggcag cactgcttaa
     2881 atttcttacc actaccgcac gggcaaggat cgttacgccc cggtttctct tctgctttga
     2941 tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt agatcaagcg
     3001 ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt ttctctaccc
     3061 gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt tttaacgaat
     3121 cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag caccactcct
     3181 caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc tcaaactgct
     3241 ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc agattcataa
     3301 agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca ccccacacgg
     3361 caaccagcca ctgttccggt tcaatctctt gcggagaact caacaccgcc gtcaataaac
     3421 cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc agaatatcgt
     3481 ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgattt tccttgtgga
     3541 tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact ttttgagtgg
     3601 ctttgctggt gattaaaaat taaggagggt gtaacgacaa gttgcaggca caaaaaaacc
     3661 acccgaaggt ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg
     3721 gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg
     3781 tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa
     3841 ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc
     3901 aggcctttca gccagacatc cgcttgaggc cgatgtcttt taaactggag cgggaaacga
     3961 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
     4021 cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat cagtgccgcc
     4081 gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt ttcaccactt
     4141 ttgatcgttt gctgaaaatt acgccgaaac gatcactgat caagcaaatc tgcacgcgca
     4201 gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca
     4261 ataccggcgt actcaaccca aatgttcgga cgccacagca gccaggccaa cgccaccatc
     4321 tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg tttacccaac
     4381 tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat cgttgccgcc
     4441 ggtagggtca cccaccagct gtgataatgc tcggttacca gcaccatggc gatagccacg
     4501 agaactttat ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg
     4561 cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg
     4621 gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg gataaggatg
     4681 acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg taactatctg
     4741 ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac gaccagaaga
     4801 tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctcg acgctggcgt
     4861 tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt tttggcccga
     4921 cgccttcaat ggtttccagg gaactggtat ttttgacctt cgcccgtttt ttacggtgcc
     4981 cgccaatcgc gtgatcatgt gattcatcgc gaatatgctg gataacatgc agcgcgggtg
     5041 aatctggcgg caaactaaat ccctcacctt ccggctcaaa gaacagcgtt tccagtccag
     5101 ccttacgatc tgctcctttg gcaacgccaa gtagcagcgg atgattttta tcccatgaga
     5161 catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa
     5221 ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga cgcagcacct
     5281 gattcatcgc cgcataatca tcgcccggcg tgatgcctgt aatgttatag cgccgatact
     5341 ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt tgttcgccca
     5401 tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc aacacgctgg
     5461 caagcgcggt cagtcgctgg tgaacggtag attgctgcga aagtttgctg gttaaggccg
     5521 tcgccgcatt ggtgcgcgcg agtttcagat aacgcgccct atcgccgcga ggtttggttt
     5581 gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc gttttatcgc
     5641 taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg ccttgtaaat
     5701 agaactgacc tacgaaggtt tctaccacct cgctcagttc cgtaccgcca ggcactttcg
     5761 ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg tggacacaag
     5821 ccatgcccgc atcgaacgcc acaccaataa cgtcaaggtc gtcgccggta ttggaaacaa
     5881 actgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt
     5941 caaactccag attctggctg gcagtttcca tacgactaat gagttgcgta agcacctgat
     6001 catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat tcttcttcac
     6061 tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctgatattgc agacacggac
     6121 gcgagcgatt gcgataaaca ctattttcgc actggcgaat ggggaaaatc ttttgcagta
     6181 gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat tcacctttgg
     6241 catgcttcgc accacgatgc atcgccagac gcgggtgggt atcgccactc aggaagataa
     6301 aaggataaga tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt
     6361 agttgtgttc cagcaacagc gcttcggttt ctgtatgagt aaccgttaca tcaatttgct
     6421 ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg aaatagctgg
     6481 aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca ccagcatcgt
     6541 acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt gcgtcaaact
     6601 gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa ttgccaggtg
     6661 agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc ggtagctgtt
     6721 caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct ggcccttggt
     6781 gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc tttctgtttt
     6841 ttctggttcg atctggctta acgccatttg ttgagcgatg tcagaagcaa tgtaacgctg
     6901 ccctgaatag acagaacgaa tcgcactcac gacttcctgc ggagccgcgc ctttgctgag
     6961 gtagcccgca gcaccggcct gcatgacttt cgctggtaaa gggttttctg tatggacggt
     7021 aagcatgatg attttgacat cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc
     7081 gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg tccggcacca
     7141 cttaaccgcg tcttcaccgc acgatgcctc accgacgact tttataccct ttatatcttc
     7201 cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat
     7261 caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt cgtatattag
     7321 cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt tctctcgttt
     7381 ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt accggatcgt
     7441 ttcacagtaa gccctacgat gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa
     7501 atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa aaacattcat
     7561 gataaaaata tttattggtc attatattaa cgtattttat agcactgccg atatcacgct
     7621 caaaaaacaa ccactgctat ttttagcaaa gcttatggta tactccgccg ccttaacatt
     7681 tttcaccgca aattttcatt gcaacatgac gaggaaaata aatgagtacg cctgattttt
     7741 ctactgccga gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga
     7801 cgctgatgct acaggcgatg ggacaagctg acgcgggccg cgtgatgtta aagatggaaa
     7861 aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt attttccaaa acggttaagc
     7921 aaattaaaca agcctaccgt cagtaatata aaaccggctg atagcgtgcc tttcagccgg
     7981 tttttgcatc tggcacgcag gacagaaaag agatcaaatt aagccagtag cggccgcgta
     8041 acaggcaacc tgggtcttat ttggtgcatt gatttttttc tgcatgtttt tctgatggaa
     8101 attgaccgta ttctcagaga ttgacaaaat catcgctatc tctgctgatg tcttcccttc
     8161 cgccgtccat ttcaaaattt ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac
     8221 tatttcatca tttaaacgca tcagagccat cagactttcg cgcaccagta actgcatttt
     8281 taattgcagt tcatcactaa gaatgggtat ttcgcgcgcg ctgctacggg aaaaggacaa
     8341 aaagcccagc gcccggtttg gcagcattaa atactgagtg acaccgcggc gtaaaccatg
     8401 tgcgcgcgcg gcttcccata acggctgtgc ttcgctgaat aagtcatcat tccacattaa
     8461 atggccctga ctaaagtttt cagggttcag caccggatca attgcgagaa agttttttgc
     8521 ctgataataa ctaacccacg cctcagggta attggtgtag aaagccactt taggtcgcgt
     8581 gaatggtacc gggtggcgaa cacataacga atagtaatcg tactccagct gctgagcctg
     8641 aaactctatt tcatggtaga cctcttctgc ggcctccatc ctctgaaaac gcaacagcat
     8701 cgtgcgacgc cagctgaaaa aatccttatc ctgcatagta a
//
LOCUS       DAJSKJ010000160         8622 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000160 DAJSKJ010000000
VERSION     DAJSKJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.162"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(248..511)
                     /gene="rpsT"
                     /locus_tag="ON345_003195"
     CDS             complement(248..511)
                     /gene="rpsT"
                     /locus_tag="ON345_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR3073215.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            614..832
                     /locus_tag="ON345_003196"
     CDS             614..832
                     /locus_tag="ON345_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR3073216.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            840..1781
                     /gene="ribF"
                     /locus_tag="ON345_003197"
     CDS             840..1781
                     /gene="ribF"
                     /locus_tag="ON345_003197"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR3073217.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1824..4640
                     /gene="ileS"
                     /locus_tag="ON345_003198"
     CDS             1824..4640
                     /gene="ileS"
                     /locus_tag="ON345_003198"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR3073218.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4640..5134
                     /gene="lspA"
                     /locus_tag="ON345_003199"
     CDS             4640..5134
                     /gene="lspA"
                     /locus_tag="ON345_003199"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR3073219.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5242..5691
                     /gene="fkpB"
                     /locus_tag="ON345_003200"
     CDS             5242..5691
                     /gene="fkpB"
                     /locus_tag="ON345_003200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3073220.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5693..6643
                     /gene="ispH"
                     /locus_tag="ON345_003201"
                     /gene_synonym="lytB"
     CDS             5693..6643
                     /gene="ispH"
                     /locus_tag="ON345_003201"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR3073221.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQVVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6709..7623
                     /gene="rihC"
                     /locus_tag="ON345_003202"
     CDS             6709..7623
                     /gene="rihC"
                     /locus_tag="ON345_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR3073222.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAP"
     gene            7790..8611
                     /gene="dapB"
                     /locus_tag="ON345_003203"
     CDS             7790..8611
                     /gene="dapB"
                     /locus_tag="ON345_003203"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR3073223.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATMRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1964 a         2159 c         2453 g         2046 t
ORIGIN      
        1 aatatccaca ttctttgtag gggagtctgg agattgagta gatattcttg ttcataatgt
       61 atcagcagat aattctacga tttttaagcc acgaagagtt cagagagtgt aacggcatgt
      121 ctgcaagaac cagcatcttt tgactatctg gcaaccggca gtgtgttttc tcacgcatca
      181 caaaagcagc aggcataaaa aaacccgctt gcgcgggctt tttcacaaag cttcagcaaa
      241 ttggcgatta agccagtttg ttgatctgtg cagtcaggtt agccttatga cgtgcagctt
      301 tgtttttgtg gatcagacct ttagcagcct gacggtccac gatcggttgc atttcgttaa
      361 atgctttctg tgcagcagct ttgtcgccag cttcgatagc tgcgtatact ttcttgatga
      421 aagtacgcat catagagcga cggcttgcgt tgtgcttacg agccttttca gactgaatgg
      481 cgcgcttctt agctgatttg atattagcca aggtccaact cccaaatgtg ttctatatgg
      541 acaattcaaa ggccgaggaa tatgcccttt tagccttctt ttgtcaatgg atttgtgcaa
      601 ataagcgccg ttaatgtgcc ggcactcgtt acgtagtgat ggcgcaggat tctaccagct
      661 tgcggggtgt gaatacagct tttccgcgat aaaaattgca gcaggcggtc actttcttcc
      721 cgtgatttgc gccatggcaa tgaaaagcca cttctttctg atttcggtac tcaatcgccg
      781 gttaaccttg accgctgtac aaggtatact cggacgattt tcactgtttt gagccagaca
      841 tgaagctgat acgcggcata cataatctca gccaggcccc gcaagaaggg tgtgtgctga
      901 ctattggtaa tttcgacggc gtgcatcgcg gtcatcgcgc gctgttacag ggcttgcagg
      961 aagaagggcg caagcgcaac ttaccggtga tggtgatgct ttttgaacct caaccactgg
     1021 aactgtttgc taccgataaa gccccggcaa gactgacccg gctgcgggaa aaactgcgtt
     1081 accttgcaga gtgtggcgtt gattacgtgc tgtgcgtgcg tttcgacagg cgtttcgcgg
     1141 cgttaaccgc gcaaaatttc atcagcgatc ttctggtgaa gcatttgcgc gtaaaatttc
     1201 ttgccgtagg ggatgatttc cgctttggcg ctggtcgtga aggcgatttc ttgttattac
     1261 agaaagctgg catggaatac ggcttcgata tcactagtac gcaaactttt tgcgaaggtg
     1321 gcgtgcgtat tagcagcacc gctgtgcgtc aggcgctggc ggatgacaat ctggctctgg
     1381 cagaaagttt actggggcac ccgtttgcca tctccgggcg tgtagtccac ggtgatgaat
     1441 tagggcgcac tataggtttc ccgacggcga atgtaccgct acgccgtcag gtttccccgg
     1501 tgaaaggggt ttatgcggta gaagtgctgg gcctcggtga aaagccgtta cccggcgtgg
     1561 caaacatcgg aacacgccca acggttgccg gtattcgcca gcagctggaa gtgcatttgt
     1621 tagatgttgc aatggacctt tacggtcgcc atatacaagt agtgctgcgt aaaaaaatac
     1681 gcaatgagca gcgatttgcg tcgctggacg aactgaaagc gcagattgcg cgtgatgaat
     1741 taaccgcccg cgaatttttt gggctaacaa aaccggctta agcctgttat gtaatcaaac
     1801 cgaaacacgg aaccgagaat ctgatgagtg actataaatc aaccctgaat ttgccggaaa
     1861 cagggttccc gatgcgtggc gatctcgcca agcgcgaacc gggaatgctg gcgcgttgga
     1921 ctgatgatga tctgtacggc atcatccgtg cggctaaaaa aggcaaaaaa accttcattc
     1981 tgcatgatgg ccctccttat gcgaatggca gcattcatat tggtcactcg gttaacaaga
     2041 ttctgaaaga cattatcgtg aagtccaaag ggctttccgg ttatgactcg ccgtatgtgc
     2101 ctggctggga ctgccacggt ctgccgatcg agctgaaagt agagcaagaa tacggtaagc
     2161 cgggtgagaa attcaccgcc gccgagttcc gcgccaagtg ccgcgaatac gcggcgaccc
     2221 aggttgacgg tcaacgcaaa gactttatcc gtctgggcgt gctgggcgac tggtcgcacc
     2281 cgtacctgac catggacttc aaaactgaag ccaacatcat ccgcgcgctg ggcaaaatca
     2341 tcggcaacgg tcacctgcac aaaggcgcga agccagttca ctggtgcgtt gactgccgtt
     2401 ctgcgctggc ggaagcggaa gttgagtatt acgacaaaac ttctccgtcc atcgacgttg
     2461 cttttcaggc ggtcgatcag gatgcactga aagcaaaatt tgccgtaagc aacgttaacg
     2521 gcccaatctc gctggtgatc tggaccacta cgccgtggac tctgcctgcg aaccgcgcaa
     2581 tctctattgc acctgatttc gactatgcgc tggtgcagat cgacggtcag gccgtgattc
     2641 tggcgaaaga tctggttgaa agcgtaatgc agcgtatcgg cgtgaccgat tacaccattc
     2701 tcggcacggt aaaaggtgcg gagcttgagt tgctgcgctt tacccatccg tttatgggct
     2761 tcgacgttcc ggcaatcctc ggcgatcacg ttaccctgga tgccggtacc ggtgccgttc
     2821 acaccgcgcc tggccacggc ccggacgact atgtgatcgg tcagaaatac ggcctggaaa
     2881 ccgctaaccc ggttggcccg gacggcactt atctgccggg cacttatccg acgctggatg
     2941 gcgtgaacgt cttcaaagcg aacgacatcg tcgttgcgct gctgcaggaa aaaggcgcgc
     3001 tgctgcacgt tgagaaaatg cagcacagct atccgtgctg ctggcgtcac aaaacgccga
     3061 tcatcttccg cgcgacgccg cagtggttcg tcagcatgga tcagaaaggt ctgcgtgcgc
     3121 agtcactgaa agagatcaaa ggcgtgcagt ggatcccgga ctggggccag gcgcgtatcg
     3181 agtcgatggt tgctaaccgt cctgactggt gtatctcccg tcagcgcacc tggggcgtac
     3241 cgatgtcact gttcgtgcac aaagacacgg aagagctgca tccgcgtact ctcgaactaa
     3301 tggaagaagt ggcaaaacgc gttgaagttg acggcatcca ggcgtggtgg gatcttgatg
     3361 cgaaagagat cctcggcgat gaagctgatc agtacgtgaa agtgccggac acattggatg
     3421 tatggtttga ctccggatct acccactctt ctgttgttga cgtgcgtccg gaatttgccg
     3481 gtcacgcagc ggacatgtat ctggaaggtt ctgaccagca ccgtggttgg ttcatgtctt
     3541 ccctaatgat ctccaccgcg atgaaaggca aagcgccgta tcgtcaggta ctgacccacg
     3601 gctttaccgt ggatggtcag ggccgcaaga tgtctaaatc catcggcaat accgtttcgc
     3661 cgcaggatgt gatgaacaaa ctgggcgcgg atattctgcg tctgtgggtg gcatcaaccg
     3721 actacaccgg tgaaatggcc gtttctgacg agatcctgaa acgtgctgcc gatagctatc
     3781 gtcgtatccg taacaccgcg cgcttcctgc tggcaaacct gaacggtttt gatccagcaa
     3841 aagatatggt gaaaccggaa gagatggtgg tactggatcg ctgggccgta ggttgtgcga
     3901 aagcggcaca ggaagacatc ctcaaggcgt acgaagcata cgatttccac gaagtggtac
     3961 agcgtctgat gcgcttctgc tccgttgaga tgggttcctt ctacctcgac atcatcaaag
     4021 accgtcagta caccgccaaa gcggacagtg tggcgcgtcg tagctgccag actgcgctgt
     4081 atcacatcgc agaagcgctg gtgcgctgga tggcaccaat cctctccttc accgctgatg
     4141 aagtgtgggg ctacctgccg ggcgaacgtg aaaaatacgt cttcaccggt gagtggtacg
     4201 aaggcctgtt tggcctggca gacagtgaag cgatgaacga tgcgttctgg gacgagctgt
     4261 tgaaagtgcg tggcgaagtg aacaaagtca ttgagcaagc gcgtgccgac aagaaagtgg
     4321 gtggctcgct ggaagcggca gtaaccttgt atgcagaacc ggaactgtcg gcgaaactga
     4381 ccgcgctggg cgatgaatta cgatttgtcc tgttgacctc cggcgctacc gttgcagact
     4441 ataacgacgc acctgctgat gctcagcaga gcgaagtact caaagggctg aaagtcgcgt
     4501 tgagtaaagc cgaaggtgag aagtgcccac gctgctggca ctacacccag gatgtcggca
     4561 aggtggcgga acacgcagaa atctgcggcc gctgtgtcag caacgtcgcc ggtgacggtg
     4621 aaaaacgtaa gtttgcctga tgagtcaatc gatctgttca acagggctac gctggctgtg
     4681 gctggtggta gtcgtgctga ttatcgatct gggcagcaaa tacctgatcc tccagaactt
     4741 tgctctgggg gatacggtcc cgctgttccc gtcgcttaat ctgcattatg cgcgtaacta
     4801 tggcgcggcg tttagtttcc ttgccgatag cggcggctgg cagcgttggt tctttgccgg
     4861 tattgcgatt ggtattagcg tgatcctggc agtgatgatg tatcgctcga aggccacgca
     4921 gaagctaaac aatatcgctt acgcgctgat tattggcggc gcgctgggca acctgttcga
     4981 ccgcctgtgg cacggcttcg ttgtcgatat gatcgacttc tacgtcggcg actggcactt
     5041 cgccaccttc aaccttgccg atactgccat ctgtgtcggt gcggcactga ttgtgctgga
     5101 aggtttttta ccttctaaag cgaaaaaaca ataataaacc ctgccggatg cgatgctgac
     5161 gcatcttatc cggcctacag attgctgcga aatcgtaggc cgcatccggc aaaaatcctc
     5221 aaaatataag agcaaacctg catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg
     5281 cacttcacgc taaaactcga cgatggcacc accgccgagt ctacccgcaa caacggtaaa
     5341 ccggcgctgt tccgcctggg cgatgcttct ctttctgaag ggctggagca acacctgttg
     5401 gggctgaaag tgggcgataa aaccaccttc tcgctggagc ccgatgcggc gtttggcgtg
     5461 ccgtcaccgg acctgattca gtacttctcc cgccgtgaat ttatggatgc aggcgagcca
     5521 gaaattggcg caatcatgct ttttaccgca atggatggca gtgagatgcc tggcgtgatc
     5581 cgcgaaatta acggcgactc cattaccgtt gatttcaacc atccgctggc cgggcagacc
     5641 gttcattttg atattgaagt gctggaaatc gatccggcac tggaggcgta acatgcagat
     5701 cctgttggcc aacccgcgtg gtttttgtgc cggggtagac cgcgctatca gcattgttga
     5761 aaacgcgctg gccatttacg gcgcaccgat ttatgtccgt cacgaagtgg tgcataaccg
     5821 ctacgtggtc gatagcttgc gcgagcgtgg ggctatcttt attgagcaga ttagcgaagt
     5881 gccggacggc gcgatcctga ttttctccgc acacggtgtt tctcaggtgg tacgtaacga
     5941 agcgaaaagc cgtgatttga cggtattcga cgccacctgc ccgctggtga ccaaagtgca
     6001 tatggaagtc gcccgcgcca gtcgccgtgg tgaagagtcg attctcatcg gccacgccgg
     6061 gcatccggaa gtggaaggta cgatgggcca gtacagtaac ccggaagggg gaatgtatct
     6121 ggtcgaatcg ccggacgatg tgtggaaact gacggtcaaa aacgaagaga agctctcctt
     6181 tatgacccag accacgctgt cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa
     6241 acgcttcccg aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca cgactaaccg
     6301 tcaggaagcg gtacgcgccc tggcagaaca ggcggaagtt gtgttggtgg tcggttcgaa
     6361 aaactcctcc aactccaacc gtctggcgga gctggcccag cgtatgggca aacgcgcgtt
     6421 tttgattgat gatgcgaaag atatccagga agagtgggtg aaagaggtta aatgcgtcgg
     6481 cgtgactgcg ggcgcatcgg ctccggatat tctggtgcag aatgtggtgg cacgtttgca
     6541 gcagctgggc ggtggtgaag ccattccgct ggaaggccgt gaagaaaaca ttgttttcga
     6601 agtgccgaaa gagctgcgtg tcgatattcg tgaagtcgat taagtcatta gcagcctaag
     6661 ttatgcgaaa atgccggtct tgttaccggc attttttatg gagaaaacat gcgtttacct
     6721 atcttcctcg atactgaccc cggcattgac gatgccgtcg ccattgccgc cgcgattttt
     6781 gcccccgaac tcgacctgca actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa
     6841 accacccgca acgccctgca actgctgcat ttctggaatg cggagattcc gctcgcccag
     6901 ggagccgctg tgccgctggt acgcgcaccg cgtgatgcag catctgtgca cggcgaatcg
     6961 ggaatggctg gctacgactt tgttgagcac aaccgaaagc caatcgggat accagcgttt
     7021 ctggcgattc gtgatgccct gatgcgtgca ccagagcctg ttaccctggt ggccattggc
     7081 ccgttaacca atattgcgct gttactttca caatgcccgg aatgtaaacc gtatattcgt
     7141 cgtctggtga tcatgggcgg ttctgctggt cgcggcaact gtacaccaaa tgccgagttt
     7201 aatatcgctg ccgatccaga agcggctgcc tgtgtcttcc gtagcggaat cgaaatcgtc
     7261 atgtgcggtc tggatgtcac caatcaggca atattaaccc ctgactatct cgctactttg
     7321 ccggagttga atcgcaccgg aaaaatgctt catgcactgt ttagccacta ccgtagcggc
     7381 agtatgcaaa gcggtctgcg aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg
     7441 gacttattca ccctcaagcc gtgttttgtc gcggtagaaa ctcaaggcga atttacctca
     7501 ggcacgacgg tggttgatat cgacggttgc ctgggcaaac cagccaatgt aaaggtggca
     7561 ttggatctgg atgtgaaagg cttccagcag tgggtggctg aggtgctggc tctggcgccg
     7621 taacctgtca catgttattg gcatgcagtc attcatcgac tcatgccttt cactgatatc
     7681 cctccctgtt tatcattaat ttctaattat cagcgttttt ggctggcggc gtagcgatgc
     7741 gctggttact ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc
     7801 aaacatccgc gttgccatcg cgggagccgg ggggcgtatg ggccgccagt tgattcaggc
     7861 ggcgctggca ttagaaggcg tgcagctggg cgcggcgctg gagcgcgaag gatcttcttt
     7921 actgggcagc gacgccggtg agctggcggg tgcaggaaaa acaggcgtta ctgtgcaaag
     7981 cagccttgat gcgataaaag atgattttga tgtgtttatc gattttaccc gtccggaagg
     8041 tacgctgaac catctcgctt tttgtcgcca gcatggcaaa gggatggtga tcggcactac
     8101 ggggtttgac gaagccggta aacaggcgat tcgtgacgcc gccgccgata ttgcgattgt
     8161 ctttgccgcc aattttagcg ttggcgttaa cgtcatgctt aagctgctgg agaaagcagc
     8221 caaagtgatg ggtgactaca ccgatatcga aattattgaa gcacatcata gacataaagt
     8281 tgatgcgccg tcaggcaccg cactggcaat gggagaggcg atcgcccacg cccttgataa
     8341 agatctgaaa gattgcgcgg tctacagtcg tgaaggccac accggtgaac gtgtgcctgg
     8401 caccattggt tttgccacca tgcgtgcagg tgacatcgtt ggtgaacata ccgcgatgtt
     8461 tgccgatatt ggcgagcgtc tggagatcac ccataaggcg tccagccgta tgacatttgc
     8521 taacggcgcg gtaagatcgg ctttgtggtt gagtggtaag gaaaatggtc tttttgatat
     8581 gcgagatgta cttgatctca ataatttgta accacaaaat at
//
LOCUS       DAJSKJ010000161         8609 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000161 DAJSKJ010000000
VERSION     DAJSKJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.163"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..779
                     /gene="yneK"
                     /locus_tag="ON345_003204"
                     /pseudo
     CDS             1..779
                     /gene="yneK"
                     /locus_tag="ON345_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            929..2119
                     /gene="ydeA"
                     /locus_tag="ON345_003205"
     CDS             929..2119
                     /gene="ydeA"
                     /locus_tag="ON345_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR3073224.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMILGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLTLLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPATLE
                     EQTQ"
     gene            complement(2144..2809)
                     /gene="marC"
                     /locus_tag="ON345_003206"
     CDS             complement(2144..2809)
                     /gene="marC"
                     /locus_tag="ON345_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR3073225.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3021..3455
                     /gene="marR"
                     /locus_tag="ON345_003207"
     CDS             3021..3455
                     /gene="marR"
                     /locus_tag="ON345_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR3073226.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3475..3858
                     /gene="marA"
                     /locus_tag="ON345_003208"
     CDS             3475..3858
                     /gene="marA"
                     /locus_tag="ON345_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR3073227.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3890..4108
                     /gene="marB"
                     /locus_tag="ON345_003209"
     CDS             3890..4108
                     /gene="marB"
                     /locus_tag="ON345_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR3073228.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4165..5604)
                     /locus_tag="ON345_003210"
     CDS             complement(4165..5604)
                     /locus_tag="ON345_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3073229.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5629..7302)
                     /locus_tag="ON345_003211"
                     /pseudo
     CDS             complement(5629..7302)
                     /locus_tag="ON345_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
     gene            complement(7358..7669)
                     /locus_tag="ON345_003212"
     CDS             complement(7358..7669)
                     /locus_tag="ON345_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3073230.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(7697..>8609)
                     /locus_tag="ON345_003213"
     CDS             complement(7697..>8609)
                     /locus_tag="ON345_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3073231.1"
                     /translation="AFLTTKYLGAQGLFVAVIVALITSEIFCRLARNPKITITMPAAV
                     PPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDLIYTLIQTPLKHMGTNIFAV
                     IILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHGTTWGAPYPITWTSI
                     NDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNINEPIMFGLPIVLNP
                     IMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAFLGTGGNWLALLVGF
                     LCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT         2324 a         2071 c         1856 g         2358 t
ORIGIN      
        1 atttatttag ataattttcg ccatgatgga gaaattaata tttttgcgct cccaacccaa
       61 agcctgtcac tatggggcgc gtatttcaga ccggcgtcga gcgtgtgctc tttctgtttc
      121 tgaatgattt tattgaacaa tttccaatga tcaaccttgg cgttcccatc aaaagagcgc
      181 atacaccaca tattgacccc ctgcctccgg atcaccatac cgctgcagat tacttacgcc
      241 agtttgattt gcttgtcctg aattttatct ctcgcggtaa ttttgtcata ctcccccgat
      301 tatggagtaa ctctgaggtt cacagatggt tcgtcaataa agatcctaac ttgatcaccg
      361 ccattctcga cataacagac agcgaattaa aagaggattt attgcaaagc ctgatggatt
      421 cattaggttc taacaaacat gtactacccg aagtctgcat ctgcttttta tcccttttag
      481 cagaacaaga atctcctcat tttcaggact tgtttttatt tttcgccaat atgttattgc
      541 actatcacca atttatgaat cccaatgaaa gtgatttgaa tgacgtgttg atgccagcat
      601 cattaagtga tgataaaatt atcaaacata tggcgcgcag gaccctcaaa ctttttgtaa
      661 aaaatgaaac gccgccaaaa gttactcacg aagacctggt gaaaaacagg cctcgctccc
      721 ctgtcagacc acctataccc gcaaccgcca aaacgccaga cctccctgaa cgtcattaaa
      781 ccgtgatgtt accgactctc tgacgcggaa cagaatcagc gtcagagaaa cggaaaacgc
      841 gatccagatc acaaatgcat tgtattcaca tcattaaccg ttttaagatc atttcatcac
      901 tttttcgcaa ctcacccgat aatctgttat gacaacaaac actgtttccc gcaaagtggc
      961 gtggctacgg gtcgttacgc tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt
     1021 ccctgttggc ctgctctctg acattgcgca aagttttcac atgcaaaccg ctcaggtcgg
     1081 cattatgttg accatttatg catgggtagt ggcgctaatg tcattgcctt ttatgttaat
     1141 gaccagccag gttgaacggc gcaaattact gatctgcctg tttgtggtgt ttattgccag
     1201 ccatgtactg tcgtttttat cgtggagctt taccgttctg gtgatcagtc gcattggtgt
     1261 ggcttttgcg catgcaattt tctggtcgat tacggcgtct ctggcgatcc gtatggctcc
     1321 ggccgggaag cgagcacagg cattgagttt aattgccacc ggtacagcac tggcgatgat
     1381 cttaggttta cctctcgggc gcattgtggg ccagtatttc ggttggcgaa tgaccttctt
     1441 cgcgattggt attggggcgc ttatcaccct tttgtgcctg attaagttac ttcccttact
     1501 gcccagtgag cattccggtt cattgaaaag cctcccgcta ttgttccgcc gcccggcatt
     1561 gatgagcatt tatttgttaa ctgtggtggt tgtcaccgcc cattacacgg catacagcta
     1621 tatcgagcct tttgtgcaaa acattgcggg attcagcgcc aactttgcca cggcattact
     1681 gttattactc ggtggtgcgg gcattattgg cagcgtaatt ttcggtaaac tgggtaatca
     1741 gtatgcgtct gcgttggtaa gtacggcgat tgcgctgttg ctggtgtgcc tgacattgct
     1801 gttacctgcg gcgaacagtg aaatacacct cggggtgctg agtattttct gggggatcgc
     1861 gatgatgatc atcgggcttg gtatgcaggt taaagtgctg gcgctggcac cagatgctac
     1921 cgacgtcgcg atggcgctat tctccggcat atttaatatt ggaatcgggg cgggtgcgtt
     1981 ggtaggtaat caggtgagtt tgcactggtc aatgtcgatg attggttatg tgggcgcggt
     2041 gcctgctttt gccgcgttaa tatggtcaat cattatattt cgccgctggc cagcgacact
     2101 cgaagaacag acgcaatagt tgaaaggccc attcgggcct tttttaatgg tacgttttaa
     2161 tgatttccag gatgccgtta ataataaact gcacacccat acataccagc aggaatccca
     2221 tcagacggga gatcgcttca atgccaccct tgcccaccag ccgcataatt gcgccggagc
     2281 tgcgtaggct tccccacaaa ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca
     2341 tcagtaccca atcagcgaag gttgaactct gacgcactgt ggacgccgag ctaataatca
     2401 tcgctatggt tcccggaccg gcggtacttg gcattgccag tggtacaaaa gcaatattgg
     2461 cactgggttc atcttccagc tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt
     2521 tctgttgcgg aaagagcatc cgaaaaccga taaacgcgac gattaagccg cctgcaattc
     2581 gcagaccggg aatcgaaatg ccaaatgtat ccatcaccag ttgcccggcg taatacgcca
     2641 ccatcatgat ggcaaatacg tacaccgagg ccatcaacga ctgacgatta cgttcggcac
     2701 tgttcatgtt gcctgccagg ccaagaaata gcgcgacagt tgttaatggg ttagctaacg
     2761 gcagcaacac caccagcccc aggccaattg ctttaaacaa atctaacatt ggtggttgtt
     2821 atcctgtgta tctgggttat cagcgaaaag tataaggggt aaacaaggat aaagtgtcac
     2881 tctttagcta accttgcatc gcattgacca aaacttgaac cgatttagca aaacgtggca
     2941 tcggtcaatt catttatttg acttatactt gcctgggcaa tattatcccc tgaaactaat
     3001 tacttgccag ggcaactaat gtgaaaagta ccagcgatct gttcaatgaa attattccat
     3061 tgggtcgctt aatccatatg gttaatcaga agaaagatcg tctgcttaac gagtatctgt
     3121 ctccgctgga tattaccgcg gcacagttta aggtgctctg ctctatccgc tgcgcggcgt
     3181 gtattactcc ggttgaactg aaaaaagtgt tgtctgtcga ccttggagca ctgacccgta
     3241 tgctggatcg cctggtctgt aaaggctggg ttgaaaggtt gccgaacccg aatgataagc
     3301 gcggcgtact ggtaaaactt accaccagcg gcgcggcaat atgcgaacaa tgccatcaat
     3361 tagttggcca ggacctgcat caagaattaa caaaaaacct gacggcggac gaagtggcaa
     3421 cacttgagca tttgcttaag aaagtcctgc cgtaaacaaa aaagaggtat gacgatgtcc
     3481 agacgcaata ctgacgctat taccattcat agcattttgg actggatcga ggacaacctg
     3541 gaatcgccac tgtcactgga gaaagtgtca gagcgttcag gttactctaa atggcacctg
     3601 caacggatgt ttaaaaaaga aaccggtcat tcattaggcc aatacatccg cagccgtaag
     3661 atgacggaaa tcgcgcaaaa gctgaaggaa agtaacgagc cgatactcta tctggcagaa
     3721 cgatatggct tcgagtcgca acaaactctg acccgaacct tcaaaaatta ctttgatgtt
     3781 ccgccgcata aataccggat gaccaatatg cagggcgaat cgcgcttttt acatccatta
     3841 aatcattaca acagctagtt gataacgtga caacgtcact gaggcaatca tgaaaccact
     3901 ttcatccgca atagcagctg cgcttattct cttttccgcg cagggcgttg cggaacaaac
     3961 cacgcagcca gttgttactt cttgtgccaa tgtcgtggtt gttcccccat cgcaggaaca
     4021 accaccgttt gatttaaatc acatgggtac tggcagtgat aagtcggatg cgctcggcgt
     4081 gccctattat aatcaacacg ctatgtagtt attgtcaggc tctgatcaca gagcctgaca
     4141 tctccgtaat atcttcctga agtctcaaat attttcgcca ttgttggcga tgagatcccg
     4201 ataccagtaa aacgacgctt tacgtatccg ttccagcgtt cccttccctt cgttgtcttt
     4261 atcgacataa atcattccgt agcgcttttt catttcccct gttccggcag aaaccaggtc
     4321 aatgcagccc cacggggtgt agccaattaa gtcaacacca tcttcgacta cggctttttt
     4381 catttcccga atatgggaag caaagtaatc aatgcgatag tgatcgttca ccgtgccgtc
     4441 agcttgtctc tggtcaaccg caccaaatcc attttcgaca ataaacagcg gcaactggaa
     4501 atgatcccag aaccagttga gtgaataacg tagccctgcc ggatcaattt gccatcccca
     4561 ttcggatgta tcgatataag ggttgctgac caaatctcgt ggttcaacat aatccagttg
     4621 cgggttatcg ggcgaaaatt gtgtcgtaaa agacatgtag tagctaaagc cgataaagtc
     4681 gacacaacca ctggcaagaa tcgcgttatc ttctggtgtg atatcgaggt tgaatccttt
     4741 cctggcaaag taattcagca tatgttgcgg ataatatcca cgagcatgaa catcagtaaa
     4801 ccagtaacga cgatgcattg ctttcgtggc catcatcata tcgttgggtg cacacgtcag
     4861 aggatagatg ggacacatgg cgatcataca gccaatgtta aattcaggat tgatcgattt
     4921 tccagtctgt accgccaggg cactggcaac taactcgtaa tgtaccgcct ggtacattat
     4981 ttgttcgcgc tcattgatat cttcctctgg cgaatacaag ataccggaat tagtaaatgg
     5041 acacaggctt tcgctgaaat tcacctgatt attgatttcg ttaaacgtca tccagtactt
     5101 tactttttct ttatagcgcg tgaagaccgt tgatgcgaag cggataaaaa agtcgatcag
     5161 tttacggttt cgccagccac catattttgt caccagatga taaggcatct caaaatgtga
     5221 aagcgtcacc acaggttcca ttccctgctt aaggcattca tcgaacagat catcataaaa
     5281 ttgtaaaccc tcttcattcg gctcctgttc gtcaccttgc ggaaagattc gtgtccaggc
     5341 aatggaagtt cgaaagcatt tgaatcccat ctcggcaaat aactgaatat ctgttttata
     5401 gcgatgataa aaatcaattg cttcatgatt gggataatta agcccgtcga taacgccttc
     5461 tgtcacttca cgcggcaccc cgtgagcgcc agcagtcatc acatcagcga tgctgatgcc
     5521 ttttcctcct tcattccagc caccttccaa ctgatgcgcg gctaccgcgc cgccccataa
     5581 aaaacctttt ttaaatcctg acatacacaa ctccttaact aaaatagatt accaccagac
     5641 ttccgcctgg atgccggcca taaactcgtc tttgctgttc tcattaaact taaaattcga
     5701 taattcgtta tccagaatat tgatatacgt tccatagaag cgaatttctg gtcgtgaacc
     5761 taaaatgctt tcacccactt tcaatgcatg ccagagtgtc gttttataag ccgattcatt
     5821 tagtgttaca ccctgctgag ttttgttctg ttgcttaaac cagcctaatt caagccccgt
     5881 ctgattccat gtattccaga tccaggccgg tcttattaca gtgcgaatac tgtcaaaatc
     5941 actatgagca ccactttcat aactataaac atcttcgccg tgagaataga caagcgcgtt
     6001 agccataata atattgtcag taagatacat ctcgccctga gagattaaac gctaggcgat
     6061 cccattggta tgatcaccat agtaatagcg accatgcgcc atcgtattac tggcatctga
     6121 gaaactggca aaactgctgg cataggaatt attggcaacc tgtaacgtaa attcattaaa
     6181 cgtatcgcgt tgcaggtttt gccgtaaaac agaagtcagc atccagctat ctttcatttc
     6241 aaaataactg ttgtcatttt cgttatcttg ttgttgatcc gttttattag gtgcggaata
     6301 tttagccatt aatgataatg tcgcatcatc ccataacggg atattgcgat aacgaacatc
     6361 cacagaattg gtattcatct ggctggtacg cgaaaaatca cgagagtaaa catcgacatc
     6421 atcacggctt aaggacatat caaacagccc tacaccaagt gcccagtttt caatccccac
     6481 acccgcggcg acatccgtgg ttaaggtttt ccagtccagc atctggatct catattgcgg
     6541 gagtttatgt ttgccgaccc agaagtctgc ctctggtgcg aagggtaaaa aacctcgcgt
     6601 tgtcaggtaa atatcgctga actgcatgat attttcattg gcactgtcac caaaccaggc
     6661 atcgttatac tgctcaccca cgttgccatc ataggtaacg accgcatttg ccgttttacc
     6721 gtcctgatta taaacacgct gatttaaggt caggtcaaac caaccgctca tctcattacc
     6781 aaaacgtccc agagaacccg ctgcataagt ttgtggtgag ccgtgattac cagtacccca
     6841 gcctgagcgg aagtacccct gatagctgaa cccaatatca tcctttatat atttactgat
     6901 atctttcagg gtcacgcttt cgatagtggt tttgtcgcct ttactttcaa taacggcaac
     6961 ttgctgcgat ccatttatag tatgcgctgc ggcagacgag ttctgttcac cgttttcatt
     7021 tttgatgtta tcagcgacgg gagatgtcgc tgatatttca gcaagcgagg ctgatttata
     7081 tttgttttcc gtgatgcttt tttgtaaggt cgaaagtcgg gatttatata ctcccagttc
     7141 attctgtgtt gctttcagct cttgtttatt ttgcgacaat tcattttcaa gcaattcaag
     7201 gcgctgctct atggttaatt ttgttgccat cgcgttcaat ggaaagaatg ctgtaataat
     7261 agaaaaagac aaaaggctga cgttaagcgt ctttatattc atgacttatc cttaagagta
     7321 taataatttc ctccagctat cgcactggag gaaaaaatca ggcatggagt tcttttctta
     7381 atgaaataat ttccctggct aaatcctggc ataatattgc attcatcaaa tgatcctgtg
     7441 catgaatcag aattaaatta acttttattt tgccacagcc ttcatcctga ctaattaatg
     7501 cggtctgaat cttatgtgcc tgaagacagg cagattcaga actggccatt agctgagttg
     7561 cgccctgcca gtctttttgc ccggcacaac gtatggcttc catcgcgtca gaacgtgcct
     7621 gccccgcgtt gaccagtaac tccataacaa gttcttcatc tgcaaacatc acaatctcct
     7681 tcctgggtaa atggaattat ccgttagttg tcatgttatt gactttgttg gcggcaataa
     7741 caaaaggtaa atagatcatt gtcgccacac ctaaacataa aaaaccaacc agtaaagcca
     7801 gccagttccc cccggttccg aggaaagcaa ttaaaggtcc gggcgtagtc cagggcacgg
     7861 cataagcaac cggtggaata atttccatcg aaacaaagaa gtaaccaatg gcacagttaa
     7921 caatgggaac cataataaac ggcaccatca tgatggggtt aagtacaata ggaaggccaa
     7981 acattatcgg ttcattgata ttgaaaatac cggggataaa tgacattttt gccagatcac
     8041 ggtattccgc acgcttagaa gcgataaaaa tagccaacaa taaccccaac gtcatacctg
     8101 aaccgccgca gttggcgaat gcatcattaa tagaagtcca ggtaatcgga tatggcgcgc
     8161 cccaggtagt gccatgttga gcggcccagg agagattctc cagattagcc tcagaaaaaa
     8221 cggtttctcg aattgccgat gtggtattag gtccgtggat ccccagcacc cacaggaaat
     8281 tacccacagc ccccaggata attaccgcaa agatattcgt tcccatatgt ttgagcggcg
     8341 tctggattaa tgtgtaaata agatcgttta gtcctgccgg ggatatcagt gtcaggcaat
     8401 aattaagtgc ggaaaagaac accatgacaa aaaatattgg caataaaact ttaaatgaac
     8461 gcgctaccgc aggcggtaca gctgccggca tcgttatggt gatttttggg tttcgagcta
     8521 agcgacaaaa tatttcactg gtgatcaatg caacgataac agcaacaaac aacccctgcg
     8581 cgccgagata tttggtcgtc aggaatgct
//
LOCUS       DAJSKJ010000162         8568 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000162 DAJSKJ010000000
VERSION     DAJSKJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.164"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            135..536
                     /locus_tag="ON345_003214"
                     /pseudo
     CDS             135..536
                     /locus_tag="ON345_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(520..1158)
                     /gene="ytfB"
                     /locus_tag="ON345_003215"
     CDS             complement(520..1158)
                     /gene="ytfB"
                     /locus_tag="ON345_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR3073232.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1377..1997
                     /gene="fklB"
                     /locus_tag="ON345_003216"
     CDS             1377..1997
                     /gene="fklB"
                     /locus_tag="ON345_003216"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3073233.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2306..3718
                     /gene="cycA"
                     /locus_tag="ON345_003217"
                     /gene_synonym="dagA"
     CDS             2306..3718
                     /gene="cycA"
                     /locus_tag="ON345_003217"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR3073234.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3763..4425)
                     /gene="ytfE"
                     /locus_tag="ON345_003218"
     CDS             complement(3763..4425)
                     /gene="ytfE"
                     /locus_tag="ON345_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR3073235.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4533..5498)
                     /locus_tag="ON345_003219"
     CDS             complement(4533..5498)
                     /locus_tag="ON345_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR3073236.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLVCVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(5606..6466)
                     /gene="qorB"
                     /locus_tag="ON345_003220"
     CDS             complement(5606..6466)
                     /gene="qorB"
                     /locus_tag="ON345_003220"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR3073237.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6555..6935
                     /locus_tag="ON345_003221"
     CDS             6555..6935
                     /locus_tag="ON345_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3073238.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(7051..>8568)
                     /gene="cpdB"
                     /locus_tag="ON345_003222"
     CDS             complement(7051..>8568)
                     /gene="cpdB"
                     /locus_tag="ON345_003222"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR3073239.1"
                     /translation="IDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMG
                     WDKANLSGKVTVNDITETVRKYVPEMREKGADVVVVLAHSGLSADPYKVMAENSVYYL
                     SEIPGVNAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQL
                     SNDSGKWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSA
                     DNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDP
                     ASYVEVEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIDPNSTKPQ
                     SLINWDGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINTNAERIKNLTFNGKPIDPN
                     AMFLVATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNN
                     WRLAPIAADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
BASE COUNT         1949 a         2176 c         2228 g         2215 t
ORIGIN      
        1 gctcattatg gatataacga tgcacccggg gagaaaatga cttccacgcc ttacaccgta
       61 cattcttgtt gtgcggtatg taattattct atgctgtcgg tatcggtgta ttcgttaatc
      121 agcaggttaa ccagccacaa aaagccattg agaaaattat ttattttaca tgggattatt
      181 atattgctaa tccctggttt ttaaaaattg tgcatagtga aaatcaaagt aaaggtgtgc
      241 attatgcaaa atcacagcgt ctcttagaaa ttaaccatgc gcacttgcag ttaatggaat
      301 ccttattgga tgaagggaag aaacacaata tttttaagcc agatatcgac ccattgcagg
      361 tgaatattaa tattgctgcg cttggcggat attatttgat caaccagcat acgcttggcc
      421 tggtttatca catcagtatg atttcacccc aggcactgga agccagacgt aaggtcatca
      481 aagagacaat ccttagctgg cttttggttg acccttcatc taccgcgcac gaataaaact
      541 gccatccggc tggcgggtga acaggacctg ttgattattc cccgtatcaa tggttaagcc
      601 cgtcaccacg ccgctggcgt tctggcggat tttcaccatc tgcccgtttt gcaaattact
      661 cagcggctta cccgcgcctt ctacctgcgc cattgcatag acatcggtag cgggcagtcc
      721 gtggtcgcgg aacagctgcg ccatcgtttt accgggttcg acacggtaag agcgccattg
      781 attatcaatt ccgctatctg gctggaatgg ttgtgtttgg gtagtttgcg gctgctcttc
      841 tggctggcct tcctgtatag gttctggagc gaccggagcc acctgatcag gatcattttg
      901 tggtgtgact aattgcgccc gcagctgttc ttctgtcggc ggttgtgatt gcgactgaat
      961 atctaactgc gcttcacgcg tcaccaccgg cgcgttgggc gtatcatcag atggaagcag
     1021 aaatccgacc accaatacaa tggcggcaat aatgatcccc cgacgatgca tcggcggcag
     1081 cgggtccatg aagcgaaaat tgtccggcgc gtgccagact ttctccaggg ttggttttag
     1141 ttcaaagcgc ccgggcatgg tttcctcctg ctccgcgtct tgttcctcaa tcatagccta
     1201 tgaataagct aacgctatga tgtccgtggt aaacccgcct ttattatatt catacgggat
     1261 attgctattg tttctttttc cccgggattt gtcatcattc ctgcgacaaa gttttaccca
     1321 aagaagtgtg gctgatatgc tgcccgctac ttttataccc taagaaagga aatacgatga
     1381 ccaccccaac ttttgacacc atcgaagcgc aagcaagcta cggcattggt ttgcaggtag
     1441 ggcaacaact gagtgaatct ggcctggaag ggctgctgcc agaagcactg gttgcaggta
     1501 ttgccgatgc gctggaaggc aaacatccgg ctgttccggt tgatgtggtg catcgcgcgc
     1561 tgcgtgaaat ccacgaacgc gccgatgccg ttcgtcgtca gcgtttccag gcgatggctg
     1621 cagaaggtgt gaaatacctg gaagaaaacg ccaaaaaaga aggtgtgaat agcaccgaat
     1681 ctggcctgca attccgcgtg atcaaccagg gtgaaggcgc aattccggca cgtaccgacc
     1741 gcgttcgtgt tcattacacc ggtaaactga tcgacggcac cgtgtttgac agctctgttg
     1801 ctcgtggtga acccgctgaa ttcccggtta atggcgtgat ccctggctgg attgaagcac
     1861 tgactctgat gccggtaggt tctaaatggg aactgactat cccgcaggaa ctggcatatg
     1921 gcgagcgcgg cgcaggcgca tccatccctc cgttcagcac cctggtgttt gaagtcgaac
     1981 tgctggaaat cctctaagca gcgcattctg ttcccctcga accagagggg agcaggcatt
     2041 cagcaataaa cccttcagtt tgccaaacgg cgctattttg tgttgcaaag accgcgtaag
     2101 cgtgtatttt tgtgagctgt ttcgcgttat caccgtgata tgacactcac tttaaacata
     2161 aaattaacat tagatctaaa tcttagtatt catcccgcgt attgttacct aatatcgatg
     2221 agtcccgata cagattcgtc gtatcataga ctgactaaag gccgtagagc ctgaacaaca
     2281 cagacaggta caggaagaaa aaaacatggt agatcaggta aaagtcgttg ccgatgatca
     2341 ggctccggct gaacagtcgc tacggcgcaa tctcacaaac cgacatattc agcttattgc
     2401 cattggcggt gccattggta cggggttgtt tatggggtct ggcaaaacga ttagccttgc
     2461 cgggccgtcg atcattttcg tttatatgat catcggtttt atgctctttt tcgtgatgcg
     2521 ggcaatgggg gaattgctgc tttcgaatct ggaatacaaa tcttttagtg acttcgcttc
     2581 cgatttactc gggccgtggg caggatattt caccggctgg acttactggt tctgctgggt
     2641 tgtaaccggt atggcagacg tggtggcgat cacggcttat gctcagttct ggttccccga
     2701 tctctccgac tgggtcgcct cgctggcggt gatagtgctg ctgctgacgc tcaatctcgc
     2761 caccgtgaaa atgttcggtg agatggagtt ctggtttgcg atgatcaaaa tcgtcgccat
     2821 tgtgtcgctg attgtcgtcg gcctggtcat ggtggcgatg cactttcagt caccgactgg
     2881 tgtggaagcg tcattcgcgc atttgtggaa tgacggcggc tggttcccga aaggtttaag
     2941 tggcttcttt gctggattcc agatagcggt tttcgctttc gtggggattg agctggtagg
     3001 tacaacagct gcggaaacca aagatccaga gaaatcactg ccacgcgcga ttaactccat
     3061 tccgatccgt atcattatgt tctacgtctt cgcgctgatt gtgattatgt ccgtgacgcc
     3121 gtggagttcg gtagtcccgg agaaaagccc gtttgttgaa ctgtttgtgt tggtaggttt
     3181 gcctgcggct gccagcgtga tcaactttgt ggtgctgacc tctgcggcgt cttccgctaa
     3241 cagcggcgtc ttctctacca gccgtatgct gtttggtctg gcgcaggaag gtgttgcgcc
     3301 gaaagcgttc gctaaacttt ctaagcgcgc agtacccgcg aaagggctga cgttctcgtg
     3361 tatctgtctg ctcggcggcg tggtgatgtt gtatgtgaat cctagtgtga ttggcgcgtt
     3421 cacgatgatt acaaccgttt ccgcgattct gtttatgttc gtctggacaa ttatcctttg
     3481 ctcgtacctg gtgtaccgca aacagcgtcc tcatctgcat gagaagtcga tctacaagat
     3541 gccgctcggc aagctgatgt gctgggtatg tatggcgttc tttgtgttcg tggtcgtgtt
     3601 gctgacactg gaagatgaca ctcgccaggc gctgctggtt accccgctgt ggtttatcgc
     3661 gctggggttg ggctggctgt ttattggtaa gaagcgggct gctgaactgc ggaaataacc
     3721 gcattatcat gctggatggc gcaatgccat ccagctttta gatcactcac ccgccagcgc
     3781 gcgtgggaac agtacattgt tttccaggct gatgtgatcc atcaggtcat caatcagttc
     3841 attaatgccg ttatacatcg ctttccaggt ggtgcaggct tccggcggcg gtgtgacgtt
     3901 attggtagtg tgtttgatca cttccagcag ttcacccgct tcatcgtgct cgctttccat
     3961 tacgctgatt ggccccattg cctggctgcc catgccttgt ttgatcatcg ggaagaggat
     4021 ctgctcttct ttcatcatgt ggctggaaag ctcttcatgc agcatagtca ggtattttgt
     4081 cagccctttt ggcacgctcg gtttgtcggc gtgaacgcgc tcgactttag tcgcttgcag
     4141 gatcagctcc ggcagttgct cgcggtgacg atcgtggtag cgcacgatga tatggtcaat
     4201 gatttctgcc agcggggcgc tacgccagtc tttctcaatc ggttgttcag cgagctttgc
     4261 cagttcagct tcaatgacct caacatccag ttctttacgt gccgccgcgc gcgccagcgt
     4321 ttgcttaccg ccgcagcagt aatccatatc atatttacga aacagagctg aagcgcgagg
     4381 aatagagagc gccagttcac ctaaaggttg gtcgcgataa gccatagctg atacctcatt
     4441 cttaataaga taagatgtat tttaaatgca tctttaaggc aaaagctata acccttacgt
     4501 agcagaggct tatttaactc acagccagta tgtcaacttt ctgagacaga ttcagttaaa
     4561 ggcttttccg gttttgctct gactgcaatg accacgccaa ccactaacag cgcgataccg
     4621 ctcagcgtca tcagcggcgg catttgctgg cgtatcaaaa aggtgtacag caaacctgcc
     4681 agtgtttcga aaacgatcag cggcccgaga atcactgtcg gtaatcgctg gctggcgacg
     4741 ttccagcaga gtgcgccaac ccatgagcaa agcacggcta tcgcaaccat cagactaata
     4801 aacaccagcg gacgggggcc aaaaggtagg gagaagtccg gcgtttgcgt attcagccag
     4861 taacaggcga cgagatagcc gatgagagaa accggcagtg tgaccagcgc ctgcgccgtc
     4921 gcccacatca tcggatgttt gtcgggattc tcccgcagcc agcgggcgtt gcgcagggca
     4981 taccatgccc agcagaccac ggaaactaac gctagcacga tgcctgaggt ataacgtgcc
     5041 cagtcaaaat cggggagtcc gtggtttaac tcagcaatat tcacacacac caggccgatg
     5101 ccaatacaaa ccagtgccgg ggcgagtttt ccccacgcga gtttgccgtc gcgctggcta
     5161 taaagcagat tggcaaaaac gggaatcacc accggcaggg tgccgataat catcgtggaa
     5221 acaggcgcac cagtacgttg aatggcactg gcaaggcaga agtaatagat gaggttgccc
     5281 atcatagtga gcatcaaggc ggtaagccag tcgcgacgcg ccaactgacg cagacgcacg
     5341 cgtcccagcc aggcaatggg cagcgcaatt aaccctaacg ccagataacg ccccatcgac
     5401 tgcaacatcg ccgggtattc cggtacgatc aacggcccga caaaaataag cccccacatc
     5461 aaccctgcta acagggcgta cagcacgccg ctaatcatta ctggcatcca ttggtctgtc
     5521 agaagaaagt tcaggaagca gacatgattg ctcccggcat cccgtcatta ttagagagga
     5581 tgcgggagga tgccacttta attaactagt tattaacatt aaaaagatgg cttacgcttt
     5641 cggctaacgt tgtcgttggg cggccaatca atttgctaag cgttttgctg ttatcaaaca
     5701 gaccaccttt cgatgcgcca acgtcagaat ccgccagcat atccgccagt ccgtcgggca
     5761 gtccgacgct tttcagtgcc gcggcgaaat cggcttcgct cagattttga taggtaacct
     5821 gtttgccgct ctgtttggtc agctctgccg ctaactgtgt caacgtccag gcactatcgc
     5881 ccgccagttc gtaaaccttg ccttcgtgac cggcttcgct aatcacgcgt gccgcagctg
     5941 ccgcataatc tgcccgcgtt gctgaggcga ttttgccatc gcccgccgca ccgataaata
     6001 cgccgtgttc cagtgctgcc ggggcgctgg cgaggtagtt ttcgctgtac cagccgttgc
     6061 gcagcagggt gtaaacgatg ccagaatcag ccaacatttt ctccgtctca atgtgctcat
     6121 cggcgaggcc gagcggggag gtatctgcat gtagcaggct ggtataagcg ataaatttca
     6181 cgccagccgc ctttgcggca ttaataacat tacgatgctg cggggcacgt tgacccactt
     6241 cgctggaaga gatcagcagt agtttttcca ctccctgaag tgcagatgtc agtgcggctt
     6301 catcgccgta gtcagcctga cgcacggtaa tgccttgtgc tgccagagcc tgggcttttg
     6361 ccggattacg aacgatagcc actatttggc tggcaggaac cgttttcatc aaggattcaa
     6421 taatatagtg accaagttgg ccagtggcac cagtaatagc gatcatggga agtctccatc
     6481 gttttgtctt gtgtttaaat acgctaacac ctaaacttac ttttagtaag tacgtacaaa
     6541 aaggtaagta tgaaatgagt caggttagcc tgtcgcaaca actgaaagag ggtaatctct
     6601 ttgcggaaca gtgcccgtcg cgcgaggtgt tgaaacacgt caccagccgt tggggggtgt
     6661 tgattctggt ggcgctacgc gaaggtactc atcgctttag cgacctgcgg cgcaaaatcg
     6721 gcggggtgag cgaaaagatg cttgcgcagt cgttacaggc gttagaacag gatggttttc
     6781 ttaaccgtat cgcgtatccg gtggtgccac cgcatgttga atacagcctc acgccgctgg
     6841 gcgagcaggt gagcgaaaag gttgctgcac tggcggactg gattgagttg aatttgcccg
     6901 aggtgttggc ggtgcgggat gaacgtgcgg cataactgcc ggatgcgcgg cctacggtgt
     6961 atgacttgta gggcggataa ggcgccagca tcgcatccgc caataaatgc cggatgcgat
     7021 tgatgattta taggccgaaa aagaagtgtt ttacttactc aaatccacct gataaatcgc
     7081 aaacccgata tcatcggtcg cgactttatt catcggatac tgccctttct ctttaataaa
     7141 cgctgcggct ttatctgacg gggaagtttc gaaacggata tccagtttct tatcggcagc
     7201 tatcggtgct aaacgccagt tgttatctgc cgccgggtga atttcccccg cacgtttcga
     7261 ctcatcagca atccacgctg ccagcaccga gcggttctca tccggtgaag caaaagcgat
     7321 atggctgtcg cccgtaccgg caaatttgcc gccgtaagcg cgatagttat tggtggcaac
     7381 gaggaacatg gcgttcggat caatcggctt gccattaaag gtcaggttct taatcctttc
     7441 cgcattggta ttaatcatct ggcactcgcc gtcataacgg gcaggctggg taacatcaat
     7501 ctgataattc acaccatcaa tcacatcaaa gttataggtg cggaaaccat cccagttgat
     7561 gagtgattgc ggtttcgtgc tgttaggatc aatctggtta aactgtccgg cggagcactc
     7621 cagccactct ttcacctctt taccgctggc tttcaccaca atcagcgtat tggggtagag
     7681 ataaagatcg gcggcattac ggaaggtcaa ctggcctttt tccacctcca catagcttgc
     7741 cgggtcattt ttgcgaccac caactttaaa cggtgcggca gctgaaagca ccggcagttt
     7801 tgccagatcc ggatcgccct gaatgtaatg ttcgacatac gctttttgcg cgttgttcac
     7861 tacttgcacg gtcggatcgt cctgcaccag cgccagatag ctatacatat tgtcggcaga
     7921 tttaccgatt ggcttgctga cgaactggcg tgtggcatcg tgatcggctt tgagtgtttc
     7981 taccagcttg ctgtcttccg ccgcgaggga ttttttattg gcgatgtcgt aaatcggccg
     8041 agcttccgct ttcgcctgcg tcacctgcca tttaccgctg tcattactga gttgtaagtc
     8101 gaccacccca agatgatcgc cccacatgcc cggcattacc gccggaacac cattcagcgt
     8161 gcctttggtg atatcagccc cttcgatatc agcaaaatct ttacccggga aaacggcgtg
     8221 agcatgacca aacataatgg cgttaacgcc cggaatttca ctgaggtaat aaactgagtt
     8281 ttccgccatc actttatacg gatcggcaga cagcccggaa tgtgccagaa cgacaacgac
     8341 atcggcacct ttctcgcgca tttcaggcac gtatttgcgc acggtttcgg taatatcatt
     8401 caccgtcact ttcccggata aattagcttt atcccagccc atgatttgcg gcggcacgac
     8461 gccaatatag ccaatcttca gcgtctgttt ttttccgtct ttatcgacca cttcggtatc
     8521 tttaattaaa tacggtgtaa acattggctg tttggttctg gcgtcaat
//
LOCUS       DAJSKJ010000163         8546 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000163 DAJSKJ010000000
VERSION     DAJSKJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.165"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(135..2126)
                     /gene="tkt"
                     /locus_tag="ON345_003223"
     CDS             complement(135..2126)
                     /gene="tkt"
                     /locus_tag="ON345_003223"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3073240.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2404..3162
                     /gene="loiP"
                     /locus_tag="ON345_003224"
                     /gene_synonym="yggG"
     CDS             2404..3162
                     /gene="loiP"
                     /locus_tag="ON345_003224"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR3073241.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3368..4288)
                     /gene="speB"
                     /locus_tag="ON345_003225"
     CDS             complement(3368..4288)
                     /gene="speB"
                     /locus_tag="ON345_003225"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR3073242.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4425..6401)
                     /gene="speA"
                     /locus_tag="ON345_003226"
     CDS             complement(4425..6401)
                     /gene="speA"
                     /locus_tag="ON345_003226"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR3073243.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6410..6541)
                     /gene="yqgB"
                     /locus_tag="ON345_003227"
     CDS             complement(6410..6541)
                     /gene="yqgB"
                     /locus_tag="ON345_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR3073244.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            6677..6892
                     /locus_tag="ON345_003228"
     CDS             6677..6892
                     /locus_tag="ON345_003228"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073245.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            7196..8350
                     /gene="metK"
                     /locus_tag="ON345_003229"
     CDS             7196..8350
                     /gene="metK"
                     /locus_tag="ON345_003229"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR3073246.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2079 a         2303 c         2203 g         1961 t
ORIGIN      
        1 tattacataa ttgatattta ttgcatataa atatttgtga tctacaacac gctttatcta
       61 ttgcttgtcc gcaaacggac atatcaaggt aataaaaaag gtcgccgaag cgaccttttt
      121 acccgaaatg ctaattacag cagttctttt gctttcgcaa caacgttatc aacagtgaag
      181 ccgaactctt caaacagcag ctctgccgga gcagattcac cgaaggtggt cataccgacg
      241 atagcaccgt tcaggccaac atacttgtac cagtagtcag caatacccgc ttctacagca
      301 acgcgtgcag taaccgcttt cggcagtacg gattcacggt aagcagcatc ctgcttgtca
      361 aatgcgtcgg tagacggcat ggacaccacg cgcgctttca cgccttcggc agtcagtttt
      421 tcgtaggcag caacagccag ttcaacttct gaaccggtag cgatgaaaat cagttccggc
      481 tgaccggcgc agtctttcag cacataacca ccgcgcgcga tgtttgccag ttgctcttca
      541 gttcgttcct gctgcgccag gttctgacgg gagaggatca gtgcggtcgg gccgtcctga
      601 cgctcaacac cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgta
      661 gacatgttcg gggttacgcg cagagaagcg acctgctcaa ccggctggtg agtcgggccg
      721 tcttcgccca gaccgatgga gtcgtgggtg taaaccatca cctgacgctg tttcatcagc
      781 gcagccatac gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac
      841 ggcaggaaac caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga
      901 acaccgtagt ggatgtagtt acccgcagca tcttcgttga ttgctttaga accagaccac
      961 agggtcaagt tagacggtgc caggtcagca gaaccgccga ggaattccgg caacagcgga
     1021 ccgaaagctt cgatagcatt ctgagacgct ttacggctgg cgattttcgc cgggttagcc
     1081 tgcagtttag caatgaactc ttttgctttc gcgtcgaagt cagacggcat ttcgcctttc
     1141 atacggcggg taaattcagc ggcttcctgc ggataagctt tcgcgtaagc agcgaatttc
     1201 tcgttccatg cggattcttt cgcctggcct gcttctttcg catcccactg agcatagatt
     1261 tcagacggga tttcgaacgg cgcgtatttc cagcccagct gttcgcgggt cagggcaatt
     1321 tcagcgtcgc ccagcggcgc accgtgggag tcgtgggtac cggctttgtt cggggaaccg
     1381 aaaccgatga tggttttgca cataagcagg gatggtttgt cagtcactgc gcgcgcttct
     1441 tctactgcgc gtttgatgga tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag
     1501 ccgtaagctt cgaaacgcat tgcggtgtcg tcggtgaacc agccttcaac gtgaccatcg
     1561 atagaaatac cgttgtcatc gtagaatgca atcagtttac ccagcttcag cgtacccgcc
     1621 agagagcaaa cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag
     1681 gtgtagtggt cgacaatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct
     1741 gcaatcgcca taccgactgc gttggcaata ccctgaccca gcggaccggt ggtggtttcc
     1801 acaccagcgg tataacctac ttccgggtgg cccggagttt tagagtgcag ctgacggaag
     1861 tttttcagtt cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc
     1921 atggagccgt ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc
     1981 gggttgtgtt tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg
     2041 gcacccgggt gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca
     2101 ttggcaagct ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc
     2161 ttaacgactt gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg
     2221 caagccgcgc gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg
     2281 ctcgcatgtt gcgcaatcta ctcgcccgtc cgctgcgctt ttccttatac tgagactgag
     2341 cgtcgattca cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa
     2401 aacatgaaaa ttcgcgcctt attggtagca atgagcgtgg caacggtact gactggttgc
     2461 cagaatatgg actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt
     2521 ttgagtgatg cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag
     2581 gcgacgattg cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg
     2641 ctaggcaaca atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg
     2701 aacgcctttg caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg
     2761 acggataacg aagtcgaagc ggtgatcggt cacgagatgg ggcacgtggc gttaggccat
     2821 gtgaagaaag ggatgcaggt ggcgcttggt acaaatgccg tgcgagtagc tgcggcctct
     2881 gcgggcggga ttgtcggcag tttatctcaa tcacaacttg gtgatctggg cgagaaatta
     2941 gtcaattcgc aattctccca gcgccaggaa gcagaagccg atgattattc ttacgatctt
     3001 ctgcgccaac gcggcatcag cccggcaggt cttgccacca gctttgaaaa actggcgaaa
     3061 ctggaagaag gccgccaaag ctcaatgttt gacgaccatc cggcatccgc cgaacgcgcc
     3121 cagcatattc gcgatcgcat gagcgcggat gggattaagt aaagcctggt ggtgtcgaac
     3181 gtaggtcaga taaggcgttc acgccgcatc tgacatgaat ggcacatttg ttaccttgtg
     3241 cgcattgccg gatgcgatgc tggcgcatct tatccggcct acgggtgccg aacgtaggtc
     3301 ggataaggcg ttcacgccgc atccgacatt aatggcacgt tttacccgtg cgcatcgcat
     3361 ctggtgctta ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg
     3421 ccgcagccag agcggtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca
     3481 tcccaacaat gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca
     3541 ggccgccaat cactggcgta ccggttcccg gcgcgaaagc aggatccagg cagtcgatat
     3601 caaaggtcag gtaaaccggc atatcaccca caatctgttt cacttgggcg ataacgtcat
     3661 ccacgctgcg atcgttcacc tggcaggcgt ccagcacggt aaaaccgttg tctttatcaa
     3721 actcggtacg aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg
     3781 cggtatagaa catggtgccg tggtcaaatt cacaaccgtt cgcgtaggta tcggtgtggg
     3841 cgtcaaagtg taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca
     3901 gcgtaacaaa gtggtcacca ccgaaagaga gcatacgctt accggcagcc agcagcttct
     3961 cggcgtgtgc ctgcagtttt tcgctcatct cacgggcatc gccaaaggca tataccagat
     4021 cgccgcagtc cacgacgttc aggcgctcac gcatgtcgaa attccacggg aagcgattgt
     4081 gttcccaggc aagattcgtc gaaacctgac ggatcgctgc cggaccgtgg cgtccccccg
     4141 cacgaccaga agtggccata tcgaacggca caccagtaat cacccagtca gcatcgctgt
     4201 catacggctg gaagttcatc ggcaggcgta aaaaaccaaa ggcgttggaa accagtgagt
     4261 tatcgtattg atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa
     4321 aaacccttcc gcgtcgttag gcccgacgag gaagggttgg atttgtcaca ataaattgtg
     4381 gcggattatc accgctaaat taagcggatt caagtaatac ggacttactc atcttccaga
     4441 taagtataac cgtacaaacc tgcctcgaac tcttcaagga actgttgttg cagttcagca
     4501 tcaagatcgg ttttcttcac ttgatcgcgg aactgagtta acagcgtttt cggatcgagc
     4561 tgcacatatt gcagcatatc cgcaacggta tcgccttcgt cagacagttc cacttctaca
     4621 ctaccgtcag ggaagacgaa cacgtcaacc gcttcggtat caccgaacag gttgtgcatg
     4681 ttgccgagga tctcctgata tgcgccgacc ataaagaaac cgagcatcgg cggattctct
     4741 ggatcgtact ccggcattgg cattgtcgtg gcaataccat caccatcaat atagtggtcg
     4801 atagcaccgt cagagtcaca ggtaatatcc agcagcacag cgcgacgttc cggcacttga
     4861 tccagccctt ccagcggcag aaccgggaac aactggtcga tcccccatgc gtccggcatt
     4921 gactggaaca gcgagaaatt gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg
     4981 attatcggac gatgcgcacg gttttgcgga tccagttgct tctgcacttc atggcacatg
     5041 ctcagataga gttgttctgc ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag
     5101 ccgatatgaa tgtcgtgcag atccatctga ctgtcgtgta accattcacg cagagaacgg
     5161 cgagttcccg gttcgtgcat ctcctgccag gtttcccaca tgctttgcag cgcgcgcggc
     5221 gcatcttctg caggcgcggt cggcaccgtg tattcgttac gttccacgcc gatgatatta
     5281 gacaccagca cggtgtgatg cgcagtcacc gcacgacccg attcggtgat taccgtcgga
     5341 tgcggcagac cgttttcttc acacgcatca ccaatcgccc agatgatgtt gttggcatat
     5401 tcattgaggc cgtagtttac ggagcagtca gactgcgaac gggtgccttc atagtccacg
     5461 cccagaccgc cgccgacgtc gaagcactga atattgacgc ccagcttgtg cagttccaca
     5521 tagaaacgcg ccgattcacg aacgcctgtg gcgatatcgc gaatattcgc catctgcgaa
     5581 ccgaggtgga agtgcagtag ttgcaggctg tcgagacgcc cggcttcacg cagggtttca
     5641 accagttgca gtacctgagt cgcagccagg ccgaacttcg atttttcccc gccggaggac
     5701 tgccatttac ccgagccctg cgaagccaga cgtgcacgca cgcccagacg aggaacgaca
     5761 ttcagacgtt ctgcttcatc cagcacaatg gcgatttctg acatcttctc aatgaccaga
     5821 tagaccttgt gccccatctt ctcgccaatt aatgccaggc ggatatattc gcggtcttta
     5881 taaccgttgc agacgatgac gctacgggtc atgccagcat gtgccagtac tgccatcaac
     5941 tcggctttgg aaccggcttc cagacccagc ggttcgcccg aatgaatcag ggactcaatc
     6001 acgcggcggt gctggttaac tttgatcgga taaacaagga agtaatcgcc gttatagccg
     6061 taggattccc tcgcacgttt gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc
     6121 tgtgggaaac agaacagtgc aggcagacgc tgaccctgtg cttcacgagt tttcactaac
     6181 tgcgcgagat cgacgcgagc ttccgggacg tccggatccg ggcacacgct gatgtggccc
     6241 agctcgttaa cgtcatagta gttattgccc caccaggcaa tattgtaagt acgcagcatc
     6301 ttgctggctt cctgggagct cattgcaacc tcctgcatgg agcgtagtac accgtgttcg
     6361 cccgctgacg aaggcaaacc catagacatg tcgtcagaca tagcgaacct caaattattt
     6421 tattaagtgt aaaacagtta acgactatcg cagcctggaa ctgcgataac aacccataag
     6481 cacatggatt ttccagcagt gaatgctgac gctccaactg cgcaaccggt ttctttttca
     6541 taacattatt aagcacataa ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa
     6601 ctcttgtttt tacaagagcg cccttgttca gtcctcagta actgtaacca gctcttgaat
     6661 cctgagaagc gccgagatgg gtataacatc ggcaggtatg caaagcagag atgcagagtg
     6721 cggggaacga atcttcacca gaacggtgag acaggttaag cagcagacaa cggttcatta
     6781 tttcgtatca cctccacggc cgcctgttaa gacgaaccca caagccaaaa ctctgatttc
     6841 aacccggctg gaagtggcaa cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg
     6901 ccgcgtttta taccccacaa tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc
     6961 attgtcagga aaaatttcca gccacgtttt taacagaatg agacacgatt caaaaaaaag
     7021 tggaaatcgg gtgaagaatt gacctaaaat agccatccag atgttaatcc atccataccg
     7081 attaacactc agactgccag tgtttttaac ctgcagagtc gtggtaggat ccgctaccac
     7141 agaaaatcca cacaacagtt tgagctaacc aaattctctt taggtgatat taaatatggc
     7201 aaaacacctt tttacgtccg agtccgtctc tgaagggcat cctgacaaaa ttgctgacca
     7261 aatctctgat gccgttttag acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg
     7321 cgaaacctac gtaaaaaccg gcatggtttt agttggcggc gaaatcacca ccagcgcctg
     7381 ggtagacatc gaagagatca cccgtaacac cgttcgcgaa attggctatg tgcattccga
     7441 catgggcttt gacgctaact cctgtgcggt tctgagcgct atcggcaaac agtctcctga
     7501 catcaaccag ggcgttgacc gtgccgatcc gctggaacag ggcgcgggtg accagggtct
     7561 gatgtttggc tacgcaacta atgaaaccga cgtgctgatg ccagcaccta tcacctatgc
     7621 acaccgtctg gtacagcgtc aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg
     7681 cccggacgcg aaaagccagg tgactttcca gtatgacgac ggcaaaatcg ttggtatcga
     7741 tgctgtcgtg ctttccactc agcactctga agagatcgac cagaaatcgc tgcaagaagc
     7801 ggtaatggaa gagatcatca agccgattct gcccgctgaa tggctgactt ctgccaccaa
     7861 attcttcatc aacccgaccg gtcgttttgt tatcggtggc ccgatgggtg actgcggtct
     7921 gactggtcgt aaaattatcg ttgataccta cggcggcatg gcacgtcacg gtggcggagc
     7981 attctctggt aaagatccat caaaagtgga ccgttccgca gcctacgcag cacgttatgt
     8041 cgcgaaaaac atcgttgctg ctggcctggc cgatcgttgt gaaattcagg tttcctacgc
     8101 aatcggcgtg gctgaaccga cctccatcat ggtagaaact ttcggtactg agaaagtgcc
     8161 ttctgaacaa ctgactctgc tggtacgtga gttcttcgac ctgcgcccat acggtctgat
     8221 tcagatgctg gatctgctgc acccaatcta caaagaaacc gcagcatacg gtcactttgg
     8281 tcgtgaacat ttcccgtggg aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg
     8341 tctgaagtaa tctttcttca cctgctttca aaggccagcc tcgcgctggc ctttttcttt
     8401 tacctcaccg cggtggatag gcgttcacgc cgcatccggc aaaaagctgc ccgcacaata
     8461 acatcattct tcctgaacac gttttatcgc aaattatcat cactactgaa accgattaca
     8521 ccaacaacac cagccaaata tttgta
//
LOCUS       DAJSKJ010000164         8481 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000164 DAJSKJ010000000
VERSION     DAJSKJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.166"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            22..1437
                     /gene="tnaA"
                     /locus_tag="ON345_003230"
     CDS             22..1437
                     /gene="tnaA"
                     /locus_tag="ON345_003230"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR3073247.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1528..2775
                     /gene="tnaB"
                     /locus_tag="ON345_003231"
     CDS             1528..2775
                     /gene="tnaB"
                     /locus_tag="ON345_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR3073248.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2907..4082
                     /gene="mdtL"
                     /locus_tag="ON345_003232"
     CDS             2907..4082
                     /gene="mdtL"
                     /locus_tag="ON345_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR3073249.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4057..5016
                     /gene="yidZ"
                     /locus_tag="ON345_003233"
     CDS             4057..5016
                     /gene="yidZ"
                     /locus_tag="ON345_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR3073250.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTSLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5173..5922
                     /locus_tag="ON345_003234"
     CDS             5173..5922
                     /locus_tag="ON345_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR3073251.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5944..6510
                     /gene="yieF"
                     /locus_tag="ON345_003235"
                     /gene_synonym="chrR"
     CDS             5944..6510
                     /gene="yieF"
                     /locus_tag="ON345_003235"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR3073252.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6564..7901)
                     /gene="adeP"
                     /locus_tag="ON345_003236"
     CDS             complement(6564..7901)
                     /gene="adeP"
                     /locus_tag="ON345_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR3073253.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLCDLSPCVIIVALLFILKIVFIDAH"
     gene            8066..>8481
                     /gene="yieH"
                     /locus_tag="ON345_003237"
     CDS             8066..>8481
                     /gene="yieH"
                     /locus_tag="ON345_003237"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR3073254.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDI"
BASE COUNT         2083 a         2130 c         2138 g         2130 t
ORIGIN      
        1 aaataaatga aggattatgt aatggaaaac tttaaacatc tccctgaacc gttccgcatt
       61 cgtgttattg agccagtaaa acgtactact cgcgcttatc gtgaagaagc aattattaaa
      121 tccggtatga acccgttcct gctggatagc gaagatgtgt ttatcgattt actgaccgac
      181 agcggcaccg gggcggtaac ccaaagtatg caggcagcga tgatgcgcgg cgacgaagcc
      241 tacagcggca gccgcagcta ctatgcgtta gccgagtcag tgaaaaatat ctttggttat
      301 caatatacta ttccgactca ccagggccgt ggcgcagagc aaatctatat tccggtactg
      361 attaaaaaac gcgagcagga aaaaggcctg gatcgcagca aaatggtggc attctctaac
      421 tatttctttg ataccacgca gggccatagc cagattaacg gctgtaccgt gcgtaacgtc
      481 tatatcaaag aagccttcga tacgggcgtg cgttacgact ttaaaggcaa ctttgacctc
      541 gaaggattag aacgcggtat tgaagaagtt ggcccgaata acgtgccgta tatcgttgca
      601 accatcacca gtaactctgc aggtggtcag ccggtttcac tggcaaactt aaaagcgatg
      661 tacagcatcg cgaagaaata cgatattccg gtggtaatgg actccgcacg ctttgctgaa
      721 aacgcctatt tcatcaagca gcgtgaagca gaatacaaag actggaccat cgagcagatc
      781 acccgcgaaa cctacaaata tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg
      841 gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct tctttgatgt gtacaccgag
      901 tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat atggcggcct ggaaggcggc
      961 gcgatggagc gtctggcggt aggtctgtat gacggcatga atctcgactg gctggcttat
     1021 cgtatcgcgc aggtacagta tctggtcgat ggtctggaag agattggcgt tgtctgccag
     1081 caggcgggcg gtcacgcggc attcgttgat gccggtaaac tgctgccgca tatcccggca
     1141 gaccagttcc cggcacaggc gctggcgtgc gagctgtata aagtcgccgg tatccgtgcg
     1201 gtagaaattg gctctttcct gttaggccgc gatccgaaaa ccggtaaaca actgccatgc
     1261 ccggctgaac tgctgcgttt aaccattccg cgcgcaacat atactcaaac acatatggac
     1321 ttcattattg aagcctttaa acatgtgaaa gagaacgcgg cgaatattaa aggattaacc
     1381 tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa aactgaaaga agtttaatta
     1441 atactacaga gtggctataa ggatgttagc cactctctta ccctacatcc tcaataacaa
     1501 aaatagcctt cctctaaagg tggcatcatg actgatcaag ctgaaaaaaa gcactctgca
     1561 ttttggggtg ttatggttat agcaggtaca gtaattggtg gaggtatgtt tgctttacct
     1621 gttgatcttg ccggtgcctg gtttttctgg ggtgccttta tccttatcat tgcctggttt
     1681 tcaatgcttc attccgggtt attgttatta gaagcaaatt taaattatcc tgtcggctcc
     1741 agttttaaca ccatcaccaa agatttaatc ggtaacacct ggaacattat cagcggtatt
     1801 accgttgcct tcgttctcta tatcctcact tatgcctata tctctgctaa tggtgcgatc
     1861 attagtgaaa cgatatcaat gaatttgggc tatcacgcta atccacgtat tgtcgggatc
     1921 tgcacagcca ttttcgttgc cagcgtattg tggataagtt cgttagccgc cagtcgcatt
     1981 acctcattgt tcctcgggct gaagattatc tcctttgtga tcgtgtttgg ttctttcttc
     2041 ttccaggtcg attactccat cctgcgcgat gccaccagca ccactgcggg aacgtcttac
     2101 ttcccgtata tctttatggc tttgccggtg tgtctggcgt catttggttt ccacggcaat
     2161 attcccagcc tgattatttg ctatggaaaa cgcaaagata agttaatcaa aagcgtggta
     2221 tttggttcgc tgctggcgct ggtgatttat ctcttctggc tctattgcac gatggggaat
     2281 attccgcgcg aaagctttaa ggcgataatc tcctcaggcg gcaacgttga ttcgctggtg
     2341 aaatcgttcc tcggcaccaa acagcacggc attatcgagt tttgcctgct ggtgttctct
     2401 aacttagctg ttgccagttc gttctttggt gtcacgctgg ggttgttcga ttatctggcg
     2461 gacctgttta agattgataa ctcccacggc gggcgtttca aaaccgtgct gttaaccttc
     2521 ctgccacctg cgttgttgta tctgatcttc ccgaacggct ttatttacgg gatcggcggt
     2581 gccgggctgt gcgccaccat ctgggcggtc attattcccg cagtgcttgc aatcaaagct
     2641 cgcaagaagt ttcccaatca gatgttcacg gtctggggcg gcaatcttat tccggcgatt
     2701 gtcattctct ttggtataac cgtgattttg tgctggttcg gcaacgtctt taacgtgtta
     2761 cctaaatttg gctaaatcct tcaagaagcc agccattcgc tggcttcttg cctctcagga
     2821 aatcacttat gtccaaatgg caactcgcct gatcctcctt caccacgtat gctttgcgtc
     2881 accttactat caggacgctt tagcccatgt cccgcttttt gatttgtagt tttgccctgg
     2941 ttttacttta tcccgccggg attgatatgt acctcgttgg tttaccgcgc atcgccgccg
     3001 atctcaatgc cagcgaagcg cagttgcata ttgcgttctc cgtatatctg gcggggatgg
     3061 cagctgcgat gttatttgcc ggtaaagtgg ccgatcgttc agggagaaag ccggtcgcca
     3121 tacccggcgc ggcgctattt attatcgcct cggtgttctg ttcactggct gaaaccagca
     3181 cgttatttct tgcaggccga tttctacagg ggttgggcgc aggctgttgt tacgtagtgg
     3241 cgttcgccat tttgcgcgac acgctggatg atcgacgtcg ggctaaagtg ctgtcattac
     3301 tcaacggtat tacctgcatc attccggtgt tagcgccagt gctcggacat ctgattatgc
     3361 ttaaattccc gtggcagagt ctgttctggg cgatggcaat gatgggcatc gcggtactga
     3421 tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc ccccgctgct tcggacaaac
     3481 cacgagaaaa tagcgagtcg ctgcttaatc ggtttttcct cagccgtgtt gttatcacca
     3541 ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc gccggtattg ctgatggaaa
     3601 tcatgggttt tgagcgcgga gaatacgcca ccattatggc gttgaccgct ggcgtcagca
     3661 tgaccgtttc attctccacg ccatttgcgc tgggaatttt taagccacgt acgttgatga
     3721 tcacctcgca ggtgttattc cttgcggcgg ggatcaccct tgccgtttca ccttcccatg
     3781 cggtttctct gtttggtatc acgctgattt gcgccggttt ctcggtaggt tttggcgtag
     3841 cgatgagtca ggcgttagga ccattttcat tacgcgcggg cgtagccagc tcgaccttag
     3901 gtattgcgca ggtttgcggt tcgtcactgt ggatttggct ggcagcggtg gttggtatcg
     3961 gcgcatggaa tatgctgatc gggattctga ttgcctgtag catagtgagc ctgttgctga
     4021 ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga agaaatccat caccacgctt
     4081 gatctcaatc tgctgctctg tctgcaactg ctgatgcagg agcgcagcgt gaccaaagcg
     4141 gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt cgctggcaaa gttaagagcg
     4201 tggtttgacg acccgctctt tgtgaactca ccgctggggc tgtcgcccac atcgctaatg
     4261 gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga gcaatctgct gctggataaa
     4321 ccgcaccacc agacaccgcg cggcctgaag tttgagttgg cggcggaatc gccgctgatg
     4381 atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac gttacccgca ggcgaccatc
     4441 aaactacgta actgggatta cgattcctta gatgccatta ctcgtggtga agtggatatt
     4501 ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc tgttaagctc gctaccgtta
     4561 gccattgatt atgaagtgct gtttagtgat gtaccctgcg tctggttacg caaagatcat
     4621 ccggcactgc atgaaacgtg gaatctggac acctttttac gttatccgca tatcagcatt
     4681 tgctgggaac agagcgacac ctgggcgctg gacaatgtgt tacaggagct ggggcgcgaa
     4741 cgcacgattg ctatgagcct gccggaattc gagcagtcac tgtttatggc agcgcaaccc
     4801 gacaatctgc tactggcgac cgcgccgcgc tactgtcagt actacaatca actccatcaa
     4861 ctgccgttgg ttgctcttcc tctcccgttt gacgaaagcc agcaaaaaaa gctggaagtt
     4921 ccttttaccc tgctgtggca taaacggaac agccataatc cgaagatcgt ctggttacgg
     4981 gaaaccatta aaaaccttta cgcgtcgatg gcataaccga atcgtatgaa acgcgaccca
     5041 atttcacaat aaaatgtaaa aaagttgtaa taagcttgtc tgaatcgaac ttttagccgc
     5101 tttagtctgt ccatcattcc agtaaatgat tactcttgta ttcataatgg accattaagc
     5161 atggagcgaa aaatggcgac tcactttgcc cgagggattt taacggaagg acatctgatt
     5221 tctgttcgtc tcccctccca gtgtcatcaa gaagcccgaa acattccccc tcatcgtcaa
     5281 agccgttttc tggcgtccag aggtttactc gcagaactga tgttcatgct gtatggcatt
     5341 ggcgaattgc cggaaatcgt caccctgccg aaaggtaaac cggttttcag tgataaaaat
     5401 ttgccttcgt tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa
     5461 ggtgaatgtg gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac
     5521 gcgcccgata accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat
     5581 cctaacgaag cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt
     5641 gatgttttga atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa
     5701 tgtgctcatg taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct agtgtggtcc
     5761 gtggcggtca cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc
     5821 tggaaaagcc tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt
     5881 tttgcccagc tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc
     5941 attatgtctg aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt
     6001 aatggcatgg ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg
     6061 ttaccttcca ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt
     6121 ccagcaacgg ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc
     6181 acgccggaat ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc
     6241 cgcctgccgg atcaaccgct ggcgggtaaa ccggtgttga ttcagaccag ctcaatgggc
     6301 gtgattggcg gcgcgcgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca
     6361 atggtgatga acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa
     6421 accggagaag tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt
     6481 ggtgagttta ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc
     6541 gggtttttaa tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac
     6601 agcagcgcaa cgatgattac gcacgggcta aggtcacaca gacgcccggt accaatcttc
     6661 atcacgcagt aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc
     6721 atcatcacgg cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga
     6781 gcaagacttg aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagcct
     6841 ggcaccattc ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc
     6901 actgccgtca gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc
     6961 gtaacggaag aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc
     7021 gcctgcttca tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg
     7081 ccaatcagcg taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct
     7141 gccagcccga ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc
     7201 ggcggcgcag aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc
     7261 gtcaccacga tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg
     7321 atgaagaagc cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcacc
     7381 agcgtttccg agttagcgac aatcacacct gcgtttttca gccccatcat gccaatgaac
     7441 agaccgatac cgctggtaat acccacacgc agactcaccg gaatgttggc tatcatccag
     7501 tagcgaacgc ggaaaatcgt cagtaacagc agacctatcg cgccccagaa gattgcgccc
     7561 atcccgacct gccacggcaa gcccatcgcc tgtacaacga caaaagcgaa gaacgcattc
     7621 aggcccatag cgggtgccag tgcaactggc aggttagcaa acagtcccat cataatactg
     7681 ccgaatgcag cgatcagaca ggtagtgacg aagacggcgc tggtatccat gccagcaacg
     7741 ccaagaattt gcgggttaac aaaaacgatg taaaccatcg tcaggaaggt ggtaaaaccg
     7801 gcgatcactt cggtccgtgc cgtcgtgcca tgttcgcgca gtttaaacac gcgttccagc
     7861 atcccctgac cagaagtctg ggtggtatgt tgatgactca ttatctattt ccgaacaagg
     7921 agggaaaatc cgtcgctatc gtataccaaa atgcgacaat aggcgcgttt gtgagagact
     7981 tttttattgg atttacttat acggcaacga ttgcgttgcg caaatcggct ctacgaaaac
     8041 gttaaactga ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt tcgactgcga
     8101 cggtacgctg gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga
     8161 atttggtatt acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta
     8221 cgaaattatc gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga
     8281 acacgtttac cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg
     8341 ggctggagcg ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa
     8401 taacaaaatg cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact
     8461 gttcagcggc tacgatattc a
//
LOCUS       DAJSKJ010000165         8461 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000165 DAJSKJ010000000
VERSION     DAJSKJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.167"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(34..693)
                     /locus_tag="ON345_003238"
     CDS             complement(34..693)
                     /locus_tag="ON345_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR3073255.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(857..1168)
                     /locus_tag="ON345_003239"
     CDS             complement(857..1168)
                     /locus_tag="ON345_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR3073256.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            1213..2646
                     /gene="bglA"
                     /locus_tag="ON345_003240"
     CDS             1213..2646
                     /gene="bglA"
                     /locus_tag="ON345_003240"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR3073257.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGAWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            complement(2703..3446)
                     /locus_tag="ON345_003241"
     CDS             complement(2703..3446)
                     /locus_tag="ON345_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3073258.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(3712..6585)
                     /gene="gcvP"
                     /locus_tag="ON345_003242"
     CDS             complement(3712..6585)
                     /gene="gcvP"
                     /locus_tag="ON345_003242"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR3073259.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQY
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(6704..7093)
                     /gene="gcvH"
                     /locus_tag="ON345_003243"
     CDS             complement(6704..7093)
                     /gene="gcvH"
                     /locus_tag="ON345_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR3073260.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(7117..8211)
                     /gene="gcvT"
                     /locus_tag="ON345_003244"
     CDS             complement(7117..8211)
                     /gene="gcvT"
                     /locus_tag="ON345_003244"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR3073261.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNSQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT         2011 a         2303 c         2157 g         1990 t
ORIGIN      
        1 gcgcgtctta tcaggcctac aaaggcatac ccattacgcc tgcccaatat acaaatagat
       61 cgccagaaag tggcacacac taccgccgag cacgaagccg tgccagatgg catggttgta
      121 tggaatgcgt ttgcagacgt agaaaatcac cccgagcgaa taaaccacgc cgcctaccgc
      181 cagtaaggta acgctgcccg ccgcgagctt aactgccatt tcataaatta ccaccagcga
      241 cagccagccc atcgccagat aggtcaccag agataaaatt ttgaatcggt gcgcgatggt
      301 cagtttaaac agaataccca gcaatgccag gctccagata actatcatca acccgcgcgc
      361 taacggagaa tccagcccca ccagcaaaaa cggcgtgtag gttccggcaa tcaacaggta
      421 aatagcgcaa tggtcaaatt tcttcagcca catttttgcc cgttgatgag gaatggcgtg
      481 atagagcgtc gaagcgagga acagcaggat catactgccg ccatagaggc tgtagctggt
      541 tatcgctgtg gcgctggcat tgagatccac cgcctgaacc agtagcaaca ccagcccaac
      601 gataccaaac accaacccaa tgccgtgact gacgctgttg gcaatttcct ctgccagcga
      661 atatccctgc ttaatgaggg gcttctgaac cataacttac tccggagaaa cgtacacgca
      721 catgtatacc tctccagcgt aactgagaat agttccagtg aacacctgtt agctaaaata
      781 aattctgatt tgagactaat ctcctaaaaa tcatgaaatt aaatgagaaa tttcaactaa
      841 caggcgtgag ttcaatttaa agacatttaa attcaatcac ataaaactgt gtctgaccgg
      901 gatagatgtc ggcaatgacc tttttcagtt cagtcagggt catattttcc tgctctgcat
      961 gtttttcagt cagcgtatcc agccttacgg ttgaggttgc ggtgacttca atcgtgcaaa
     1021 aataaccgtc atcttcaaaa cgcccgacac gaagcacatc acccgttttg aagtgcgatt
     1081 cagactcgtc gcggatggtg atggttttac gcccagccag aatgtcatcc tggaaacgtt
     1141 gaaaaaaagt gatgtcgttt ggctgcatgg tactatttcc tgtaagaatt gactcatctg
     1201 gagcctatga tagtgaaaaa actcacctta ccgaaagatt tcttatgggg tggcgccgtt
     1261 gccgctcatc aggtcgaagg cgcctggaac aaaggcggca aagggccgag catttgtgac
     1321 gttctgaccg gtggcgcaca cggcgtgccg cgcgaaatca ccaaagaagt cttgccagga
     1381 aaatactatc caaaccatga agccgttgat ttttatggtc actacaagga agacatcaag
     1441 ctatttgccg aaatgggctt caaatgtttt cgtacatcca tcgcctggac gcgcattttt
     1501 ccaaaaggcg atgaagctca gccaaacgaa gaagggctga agttctacga ctctctgttc
     1561 gatgaactac tgaaatacaa catcgaaccg gtgatcaccc tctcccactt tgaaatgccg
     1621 ctgcatctgg tgcagcaata cggtagctgg accaaccgta aagtggttga tttctttgta
     1681 cgtttcgcgg aagtggtatt tgaacgctat aagcacaaag tcaaatactg gatgaccttc
     1741 aacgaaatta acaaccagcg taactggcgt gcaccgctgt tcggttactg ctgctccggc
     1801 gtggtgtata ccgagcatga aaacccggaa gagacgatgt atcaggtgct gcatcaccag
     1861 tttgtcgcca gcgcactggc ggtgaaagcc gcgcgtcgca ttaacccgga gatgaaagtc
     1921 ggctgtatgc tggcgatggt gccgctctat ccctactcct gtaacccgga cgatgtgatg
     1981 tttgcccagg agtcgatgcg cgaacgctac gtctttaccg atgtgcagtt gcgtggctat
     2041 tacccgtcct atgtgttgaa cgagtgggag cgtcgcggat ttaacatcaa aatggaagat
     2101 ggcgatctgg atgtgctgcg cgaaggcacc tgcgattatc ttggtttcag ctattacatg
     2161 accaacgcag tgaaggccga aggcggcacc ggcgatgcga tctctggttt cgaaggcagc
     2221 gtaccgaacc cgtatgttaa agcgtctgac tggggctggc agattgatcc agtaggtctg
     2281 cgctatgccc tttgcgaact gtatgagcgt tatcagaggc cgctgtttat tgtcgaaaac
     2341 ggttttggcg cttacgacaa agtggaagaa gatggcagca tcaacgacga ctaccgcatt
     2401 gactacctgc gcgcccatat cgaagagatg aaaaaagcag tgacttacga tggtgtggat
     2461 ctgatgggct acacaccgtg gggctgcatc gactgcgtat cgttcaccac cgggcagtac
     2521 agcaaacgct acggctttat ctatgtgaat aaacatgacg acggtactgg cgatatgtcg
     2581 cgttcacgta agaagagctt taactggtac aaagaggtga ttgccagcaa cggcgagaag
     2641 ctttaagtcg atgaagtacc ggatgcaata cttgttgcat ccggtcagac aacttccctg
     2701 ttttatttcc cgcccgccaa atcgataaaa cttcccgtga cgtaagaggc tttatcactt
     2761 agtagccaga caatggcctg cgcgacctct tctgcctgtc caccacgctg catggggatg
     2821 ttcgacttaa cgcgatcgac gcgtccaggc tcgccgccgc tggcgtgcat ttcggtataa
     2881 ataaaccctg gccgcacgca gttaacgcgg atcccctgcg cggcgacttc cagcgatagt
     2941 ccggtggtta acgtatcaat cgcccctttc gatgccgcgt aatcaacata ttcccctggc
     3001 gaacccaacc gtgaggccac cgaagagaca ttgacgatag cgccgccact gccaccattt
     3061 ttaagcgcca tgcgttttac cgcctcgcgg cagcagagaa aatatcccgt cacgttggtg
     3121 gaaagtactc ggttgattcg ctctgcggta aggttttcaa cggtgcactg ggtaaacaag
     3181 atcccggcgt tattgaccag cgctgctagc ggttcatcgt gctgatcgat tgctgtaaac
     3241 attgcgatga cctgattttc gtcgctgata tccgcctgga gcacgaatgc cttgccaccg
     3301 gcttgcgtga ttaagttcac cacttcctgc gccgcatgga ggttttgttg ataattaacc
     3361 gccaccgtat actcttcttt cgccaacagt aatgcagttg cccgcccgat gccgcgactg
     3421 ccaccggtca caagtgctat agctatttgt ttttccgaga aaaattaatt cgttgacgta
     3481 tctttgagat tgctaacaaa ctggctcaat cttcaatgtt ggcacgcttt tcccctcgcc
     3541 ctttcaggga gagggccggg gtgagggtaa atattcgcgc cagtgccggc ctgttcccct
     3601 caccctaacc ctctccccaa aggggcgagg ggactgtctg agcacttttg tactttgtca
     3661 tctgactaaa aaggcgccga agcgccttta gaaaatagtc gaatcagtga attactggta
     3721 ttcgctaatc ggtacgcagg agcagaacag gttacggtcg ccgtaaacat catccagacg
     3781 tttcaccgtc ggccagtatt tgtctgccac gcctgccggg aataccgcaa cttcacggct
     3841 gtacggatgc gcccactcgg cgaccagttc gctctgaatg tgcggcgcgt tcaccagcgg
     3901 gttatcttcc agcggccaga caccggcttt cacctggtca atttctgcgc ggatagccag
     3961 catcgcgtcg ataaagcgat ccagttccac tttgctttca gattcagtcg gttcaaccat
     4021 cagcgtaccc gccaccggga acgacatcgt cggcgcgtgg aaaccgtagt cgatcaggcg
     4081 cttggcaata tccagctcgc tgatgccggt ttcttctttc agcgggcgaa tatcgagaat
     4141 acattcgtgc gccacgcgac cgtcgcgacc agtatacagc accgggaagg catactgcag
     4201 gcggctggca atatagttgg cgttgaggat tgccacctgg cttgcttttt tcagcccttc
     4261 tgcgcccatc atgcggatgt acatccagct gattggcagg atagaggcgc taccgaacgg
     4321 tgccgcagaa actgcgccct gacgggttaa catgccttcg atttgcacca cgctatgacc
     4381 cggtacaaac ggtgccagat gcgctttcac accgatcggt cccatacccg gaccaccacc
     4441 gccgtgcgga atgcagaaag ttttatgcag gttgaggtgc gaaacgtccg cgccaataaa
     4501 gcccggcgag gtgatgccaa cctgggcgtt catgttcgcg ccatcaaggt aaacctgacc
     4561 gccgaactga tgcacgactt cacacacttc acggatcgtt tcttcataca cgccgtgggt
     4621 agaaggataa gtcaccatga tacaggagag gttatcgccc gcctgttccg ctttcgcgcg
     4681 cagatcagtc agatcgatgt tgccgttttt atcacacgcc acaaccacca cctgcattcc
     4741 tgccatatgt gcagaagcgg ggttagttcc gtgcgcagaa gccgggatca ggcagatatc
     4801 gcgatgccct tcgttgcggc tttcatgata atgacgaatc gccagcaggc ccgcgtattc
     4861 gccctgtgcg ccggagttcg gctgcataca aacggcgtcg taaccggtca gtttcaccag
     4921 ccagtcagcc agctgcgcaa tcatctgctg ataaccttca gcctgttccg gcggacaaaa
     4981 cgggtgcagt tcggcaaatt ccggccaggt gattgggatc atctcggcgg cggcgttcag
     5041 tttcatggtg caggaaccca gcgggatcat cgcctgattc agcgccagat ctttacgctc
     5101 cagcgagtgc atatagcgca tcatttcggt ttcgctgtgg tagcgattaa acaccggatg
     5161 ggtgaggatt tcgtcgtcgc gcagcatcgc aggctggata gagcggctgt cgtgagccac
     5221 gtctttgtcc agcgtgtcga tgtccaggcc gtggttatcg cccagcagca cgttgaaaag
     5281 ctgcattacg ttttcacgcg tggttgtttc atcaagggtg atcccaaccg cgttcagaat
     5341 atcgctacgc aggttgattt cagccgcttc ggcacgcgtc agtacgcccg ctttgtcggc
     5401 cacttccaca cacaaggtgt cgaaatagtg cgcatggcgc agtttcagac ctttttgttg
     5461 caggcccgcc gccaggatat cggtcagacg gtgaatgcgg ttagcgatac gtttcaggcc
     5521 aatcgggccg tgataaacgg catacaggct ggcgatgttt gccagcagca cctgggaagt
     5581 acaaatgttg gagttcgctt tctcacggcg aatatgttgc tcgcgagtct gcatcgccat
     5641 gcgcagcgcg gtattgccag ctgcatcttt cgatacaccg ataatacggc ccggcattga
     5701 gcgtttgtat tcatctttcg ccgcaaagaa ggccgcgtgt gggccaccgt agcccatcgg
     5761 cacgccgaag cgttgcgccg aaccaaagac aatatctgcg ccctgtttac ccggcgcagt
     5821 taacagcacc agcgccataa tatcggcggc gacactgacc acaattttgc gtgatttcag
     5881 ttcgctaata agcgcggtgt agtcgtggat ttcaccggtt gtgccaacct gctgtaacag
     5941 cacgccgaag acgtcctgat ggtcgagcac tttttgcgcg tcatcgacaa tcacttcaaa
     6001 accaaaggtt tcggcacgag tacggaccac atccagcgtt tgcggatgca catcggaagc
     6061 cacgaagaag cggttggcat ttttcagttt gctgacgcgt ttcgccatcg ccattgcttc
     6121 ggcggcagcg gtggcctcgt ccagaagaga agcagaggcc atatccagtc cagtcaaatc
     6181 cagcgttacc tgctggaagt tgagcagtgc ttcaaggcgg ccctgggaga cttcaggttg
     6241 atacggagtg tacgcggtat accagcccgg attttccagc atgttacgca ggataaccgg
     6301 cggtagctgc acggcggtgt aacccatgcc gatgtaagac gtgaagcgtt tattgcgact
     6361 ggcaatagcc ttgagttctg ccagtgcggc gtattcggtc gccggtgcgc caacctgcgg
     6421 tggtgtcgca agctgaatat ctttcggcac aatctggccg gtcagcgcgt ttaacgattg
     6481 tgcaccaacg gcattcagca tttcttgctg ttgcgcggcg tccggtccga tatggcgttc
     6541 aataaaagcg ccgctgtttt caagctggct taacgtctgt gtcatgagcg atggttcctg
     6601 aaacgtgcag tgaattgtga acctctctcc ttacgaagag agtgagggtg aggcataaat
     6661 tttcctcacc ctgatcctct cccgcagaag aggaataaag ccgttactcg tcttctaaca
     6721 atgcttcgta tgcggtcgca tccagcagtg attccagttc gctttcatcg ctggctttga
     6781 ttttaaagat ccagccacct gcatacggtt cgctgttcac cagttccggg gaatcgctca
     6841 gtgcgtcgtt taccgccacg atttcaccgc ttactggcgc ataaatgtct gacgccgctt
     6901 ttactgattc ggcaaccgcg cagtcatcgc ccgcgctaac cgttgcgccc acttccggca
     6961 ggtcaacaaa caccatatcg cctaacagct cctgggcatg ttcggttata ccaacggtgt
     7021 aagtgccgtc ggcttcttta cgcagccatt cgtgttcttt gctgtatttc agttctgctg
     7081 gtacgttgct catcaatcaa tctccaaaaa agtaaatcac gcgacggctt taccgttacg
     7141 cacaaaaaca ggtttcgtca ctttaaccgg catttcacgg ttgcgaattt gcacaatcgc
     7201 cgtttcgcca ataccttccg gcacgcgtgc cagcgcaatg ctgtaaccca gcgtcgggga
     7261 gaaagtcccg ctggtgataa tgccttcatg ctggttgccc tgcgcatcgg taaaacgtac
     7321 cggcagttca ttacgcagca cgcctttttc ggtcatcacc agaccaacca gtttttctgt
     7381 gccatgctca cgctgcactt ccagggcttc acgaccgatg aagtcacgat ctgccggttc
     7441 ccaggcgata gtccagccca tgttggcggc taacggagag atggtttcgt ccatctcctg
     7501 accataaaga ttcatgcccg cttccagacg cagcgtgtca cgcgcgccca gaccgcatgg
     7561 cttaacgccc gcttccacca gcgcacgcca gaaatcggcc gctttttcat tgggcagcgc
     7621 aatttcatag cccgcttcac cggtataacc agtggtggca ataaacagat cgcccgcctg
     7681 cacgccaaag aacggtttca tcccttccac cgcctgacgc tgagcatcat taaacagtgt
     7741 ggcagctttt gcctgcgaat tcggcccttg tacggcgatc atggaaaggt catcacgaac
     7801 ggtaatttcg atgccgaaag gttcagcgtg ttgggtaatc caggagaggt ctttttcgcg
     7861 ggtggcggag ttaacaacga ggcggaagaa atcttcagta aagtagtaga cgatgaggtc
     7921 atctatcaca ccgccagagg cattcaacat ccccgagtaa agggctttgc cgcttttggt
     7981 gagcttcgcc acatcgttcg ccagcagata acgcagaaac tcccgggtgc ggctgccgcg
     8041 gagatcgacg atggtcatat gtgacacatc aaacattccg gcatcggtac gtaccgcatg
     8101 atgttcgtcg atttgcgaac cgtaatgcag cggcatcatc cagccgtgga aatccaccat
     8161 gcgagcgccg caaagcgtgt gttgttcgta caaaggagtc tgttgtgcca tcttgtcctc
     8221 attgaataag cggggctgac aacttttcat ggtgaaatta tcaccacgaa acccagcatc
     8281 ggagccactc ccggtcccca acgcaatcgt tctcttttgc ctgaacttac caccgaaaca
     8341 gactgttaac cataaggtaa aattgatcat cacattagct tatggttaaa aaacgccaaa
     8401 atcgcgacag aataaaaaac caaaaaacac accagtttct atataaagat aatgccatta
     8461 g
//
LOCUS       DAJSKJ010000166         8422 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000166 DAJSKJ010000000
VERSION     DAJSKJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.168"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(114..320)
                     /locus_tag="ON345_003245"
     CDS             complement(114..320)
                     /locus_tag="ON345_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR3073262.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            633..722
                     /gene="kdpF"
                     /locus_tag="ON345_003246"
     CDS             633..722
                     /gene="kdpF"
                     /locus_tag="ON345_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR3073263.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            722..2395
                     /gene="kdpA"
                     /locus_tag="ON345_003247"
     CDS             722..2395
                     /gene="kdpA"
                     /locus_tag="ON345_003247"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR3073264.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFFSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2418..4466
                     /gene="kdpB"
                     /locus_tag="ON345_003248"
     CDS             2418..4466
                     /gene="kdpB"
                     /locus_tag="ON345_003248"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR3073265.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASAFIPTQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     ICKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4475..5047
                     /gene="kdpC"
                     /locus_tag="ON345_003249"
     CDS             4475..5047
                     /gene="kdpC"
                     /locus_tag="ON345_003249"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR3073266.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVLGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5040..7724
                     /gene="kdpD"
                     /locus_tag="ON345_003250"
     CDS             5040..7724
                     /gene="kdpD"
                     /locus_tag="ON345_003250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR3073267.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPSLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7721..8398
                     /gene="kdpE"
                     /locus_tag="ON345_003251"
     CDS             7721..8398
                     /gene="kdpE"
                     /locus_tag="ON345_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR3073268.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAMPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1759 a         2198 c         2442 g         2023 t
ORIGIN      
        1 atatatgttt cgattggtat aacgatgtct aaatattcct ctgaacatct tcctgataca
       61 aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt caaaactcag caatcaataa
      121 aaatcaccag ttgccttttc ggcctgatcc atccacaccg gtttatcgct ggttttcgac
      181 cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag catgaaaggc
      241 agcgccagaa cgcccgctgc aaccgcataa gtacggcgta aaaagataag ccatgcagga
      301 tattctctgt agagttccat acttttctcc cccttttgta tctacccggt gaatggcacc
      361 ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa ttttactact
      421 catccgacca cttatttttg cttattgatg gtttatttac attcatcctg taattaagtt
      481 acacaaaagt taaattaata ctaaacatta gttaaatcat ggcttttgcc atttttatac
      541 tttttttaca ccccgcccgc agatttttgc gaaatctttg cagccagaat tctacccttc
      601 cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata accggcgtat
      661 tgctggtgtt tttattactg ggttatctgg tttatgccct gatcaatgcg gaggcgttct
      721 gatggctgcg caagggttct tactgatcgc cacgttttta ctggtgttaa tggtgctggc
      781 gcgtccttta ggcagcgggc tggcgcggct gattaatgac attcctcttc ccggtacaac
      841 gggcgttgag cgcgtacttt ttcgcgcact tggcgtctct gaccgtgaga tgaactggaa
      901 gcaatatctc tgtgccattc tcggcctgaa catgctgggg ctggcggtgc tgttttttat
      961 gttgctcggt cagcactatc tgccgcttaa tccacagcag ttgccggggc tgtcgtggga
     1021 tctggcgctg aataccgccg tcagctttgt cacgaatacc aactggcaat cttacagcgg
     1081 tgaaaccacg ttgagctatt tcagccagat ggctggctta acggtgcaaa actttttttc
     1141 tgccgccagc gggattgcgg tgatttttgc cctcatccgt gcgtttaccc gccagagcat
     1201 gagcacactc gggaatgcct gggtcgatct gctacgcatc acgttatggg tgctcgtccc
     1261 tgtggcgttg ttgatcgcac tgttttttat tcaacaaggt gcgctgcaaa actttcagcc
     1321 ttatcaggct gtgaataccg ttgaaggagc gcaacagctg ttacccatgg ggcctgtagc
     1381 ttctcaggaa gcgatcaaga tgctcggtac taacggcggt ggcttcttta atgccaactc
     1441 gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc tggcgatctt
     1501 cttgatccca acggcgctgt gctttgcctt tggtgaagtg acgggcgatc gccgccaggg
     1561 gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg tggtgatgtg
     1621 ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc gcagacagca gcatcaatat
     1681 ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac
     1741 ggcggcttcc tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc tcggtggcat
     1801 ggtgccgatg tggctgatgc aaattggtga agtggtgttc ggcggtgtcg gttctggtct
     1861 ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga tgattggtcg
     1921 tacaccggaa tatctgggta aaaaaatcga cgtacgcgag atgaaactga ctgcactggc
     1981 gattctggtt accccgacgc tggtgctgat gggcgcggcg ctggcaatga tgaccgacgc
     2041 cggacgtagc gccatgctca accctggccc gcatggtttt agcgaagtgc tgtacgccgt
     2101 gtcatccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca actccccgtt
     2161 ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc ttcggggtga ttatcccggt
     2221 gatggcaatt gccggttcgc tggtgagtaa aaagagccaa cccgccagct ccggcacgct
     2281 gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc tggttggcgc
     2341 actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct cctgatgata
     2401 ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca acacttgtcg
     2461 ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg
     2521 tgatgtttat cgtctggatc ggcagtctgc tgaccacctg tattagcatc gctatggcaa
     2581 gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca
     2641 ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa gcgcaggcca
     2701 acagtctgaa aggggtgaaa aaaactgcct ttgcccgcaa gctgcgtgag ccgaaatatg
     2761 gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa aggtgacatc gtgctggtag
     2821 aagctggcga tattatcccc tgcgatggtg aagtcattga aggcggcgca tcggtcgatg
     2881 aaagcgccat caccggggaa tcggcaccgg tgatccgtga atccggcggc gattttgcct
     2941 ccgtcaccgg cggtacgcgt attctttctg actggctggt gattgagtgt agcgttaacc
     3001 ccggagagac atttctggat cggatgatcg cgatggtgga aggcgcgcag cgacgtaaaa
     3061 cgccgaacga gattgccctg accatcctgc tgattgccct gactatcgtc tttttactgg
     3121 caaccgccac gctgtggccg ttttccgcgt ggggcggtaa tgcagtcagc gtaacggtac
     3181 tggtggcgct gctggtctgt ctgatcccaa ccactattgg cggcctgttg tcagcgatcg
     3241 gcgtcgccgg gatgagtcgg atgctgggcg caaatgtgat tgccaccagc ggacgtgcag
     3301 ttgaagcggc aggtgacgtt gacgttctgc ttctggataa aaccggcacc atcacactcg
     3361 gtaaccgtca ggcgtcggcg tttatcccca cgcagggcgt ggatgaaaaa acgctggctg
     3421 acgccgcaca actggcttcg ctggctgatg aaacgccgga aggccgcagt attgtgatcc
     3481 tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg
     3541 taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc atgatctgta
     3601 aaggttctgt cgatgccatt cgccgccatg ttgaggctaa cggcggtcac ttccctgccg
     3661 atgttgatca aaaagtcgat caggttgcgc gtcagggagc cacgccgctg gtggtggtgg
     3721 aaggttctcg tgtgctgggc gttattgcgc taaaagatat cgtcaaaggc ggtattaaag
     3781 agcgcttcgc ccagctgcgc aaaatgggca ttaaaacggt gatgattacc ggcgataacc
     3841 gtctgactgc cgccgccatt gctgcggaag cgggcgtcga tgattttctc gccgaagcga
     3901 caccggaggc caagctggca ttgattcgtc agtatcaggc ggaaggccgt ttggtggcga
     3961 tgaccggcga cggcaccaat gatgctccgg cgctggcgca ggcagatgtc gcggtagcga
     4021 tgaactccgg tactcaggcg gcgaaagaag cgggcaatat ggtcgatctc gactctaacc
     4081 cgaccaagtt gattgaggtg gtgcatattg gcaaacagat gctgatgacc cgtggctcgc
     4141 tgaccacctt cagtattgcc aacgatgtgg cgaaatactt cgccattatt ccggcggcgt
     4201 tcgcggcaac gtatccgcag ttaaatgcgc tgaacatcat gcgcctgcat tcgcccgact
     4261 ccgccattct cagtgcggtc attttcaacg ccttgattat cgtctttttg attcctctgg
     4321 cattaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc cgtaacttat
     4381 ggatttacgg tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc attgatttac
     4441 tgctgaccgt ttgcggtctg gtgtgaggtt taccatgagt ggattacgtc cggcattatc
     4501 gacatttatc tttctgttat tgattactgg cggtgtttac ccgctgctga ccaccgcgct
     4561 ggggcaatgg tggtttccct ggcaggccaa cggttcgttg atccgtgaag gtgatacagt
     4621 gctgggttcg gcattaatcg ggcagaattt tactggcaac ggctattttc atggtcgccc
     4681 gtcggcaacg gcagaaatgc cctataatcc acaggcttct ggcgggagca atctggcggt
     4741 cagtaaccct gagctggata aactaatagc cgcacgcgtt gctgcattac gggccgctaa
     4801 cccggatgcc agcgcgagcg ttccggttga actggtgacg gcatcggcaa gcgggctgga
     4861 caataatatc accccgcaag cggcggcctg gcaaatccca cgcgtggcga aagcgcgtaa
     4921 tctcagcgtt gaacagctca cgcaactgat cgcaaaatac agccaacaac cgctggtgaa
     4981 atatatcggc cagccggtcg tcaacattgt cgaactcaat ctggcactgg ataagcttga
     5041 tgaataacga acccttacgt cccgaccccg atcgtctgct ggaacaaact gccgcgccgc
     5101 atcgggggaa gctgaaagtt ttcttcggtg cctgtgcagg cgtcgggaag acctgggcga
     5161 tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt ggcgtggtag
     5221 aaacccacgg acggaaagat accgccgcca tgctggaagg gctggctgtt ctgccgccaa
     5281 aacgcctggc gtaccgtggg cggcatatca gcgagtttga tctcgatgcc gccctcgccc
     5341 gccgtccggc gctgatctta atggacgaac tggcgcacag taatgcgcca ggttctcgtc
     5401 atcctaaacg ctggcaggat atcgaagaac tgctggaagc tggcattgat gttttcacaa
     5461 ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt cagcggcgtc accggaattc
     5521 aggtacggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgta gtgctggtgg
     5581 acttgccccc ggacgatctg cgccagcggc tgaaagaagg caaagtctat attgccgggc
     5641 aggcggagcg cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc ctgcgtgaac
     5701 tggcactgcg ccgtactgcc gatcgcgtag atgagcaaat gcgcgcctgg cgggggcatc
     5761 ctggtgaaga gaaagtgtgg cacacgcgcg acgcgatcct tttatgcatc gggcataaca
     5821 ccggcagtga gaaactggtc cgcgcggcgg cgcggttggc ctcgcgcctg ggtagcgtct
     5881 ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt accggaaaaa aaacgtcggg
     5941 caattctcag cgccttacgt ctggcgcagg aactgggcgc ggagacggca acactttctg
     6001 atccagcaga agagaaagcg gtagtgcgtt atgcccgtga acataatctc ggcaagatta
     6061 ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga aacgtttgct gaccgactgg
     6121 cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct tgatgaacca cccgcccgca
     6181 cgattaacaa cgcgccggat agccgctctt ttaaagacaa gtggcgggta cagattcagg
     6241 gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt aattgccatg cagtggctga
     6301 tggcgtttga tgccgccaac ctggtgatgc tgtatctgct tggcgtggtg gtagtggcgc
     6361 tattttatgg acgctggcct tcggtggttg ctaccgtcat taatgtggtg agtttcgatc
     6421 tcttttttat cgccccacgc ggcacgctcg ccgtctctga tgtgcaatat ctgctgacct
     6481 tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgctggc gtgcgttatc
     6541 aggcgcgggt agcccgttac cgcgagcaac gcacacggca cttatatgaa atgtcgaaag
     6601 ctctggcggt gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa tttattgcct
     6661 ccacgtttca tgcccgcagt caggtgttgt tgcccgatga caacggtaaa ttgcagccgt
     6721 taacacatcc gcaaggaatg acgccatggg acgatgccat cgcgcagtgg agttttgata
     6781 aaggcctgcc tgcgggtgcg ggcaccgaca cgttacccgg cgtgccgtac cagattttgc
     6841 cgctaaaaag cggcgagaaa acctacggac tggtggtggt ggaaccgggg aatctgcgcc
     6901 agttgatgat cccggagcag cagcgcctgc tggagacatt tacgctgtta gtcgccaatg
     6961 cccttgagcg gctgacactg accgccagcg aagaacaggc gcggatggca agcgaacgtg
     7021 aacagatccg caacgccctg ctggcggcgc tttcgcatga tttacgcacg ccgcttacgg
     7081 tgctgtttgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga tcgccccacg
     7141 cccgccaggc cagcgagatt cgtcagcatg tgctgaacac tacccgactg gtgaataatc
     7201 tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag tggttaacgc
     7261 tggaagaagt agtcggcagc gcgctgcaaa tgctggaacc gagtttatcg tcgcccatca
     7321 atctttctct gccagaaccg ctgaccttaa tccacgttga cgggccactc tttgaacggg
     7381 tgctgattaa tctgctggag aacgcggtga aatatgcggg tgcgcaggcc gaaattggta
     7441 tcgatgccca cgttgagggc gaaaatctac aactggatgt ctgggataac ggccccggtc
     7501 ttccgccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat aaagagtcgg
     7561 cagtaccggg ggtagggctt ggactggcaa tttgtcgggc gatagtggat gtacacgggg
     7621 gcactattac cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc
     7681 agcaaaccgc tcctgaactt gaagactttc atgaggatat gtgacaaacg ttctgattgt
     7741 tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg acgggatgcg
     7801 cgtctatgag gccgaaacgc tgcaacgcgg cttgctggaa gcggctaccc gtaagccaga
     7861 tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta tccgcgacct
     7921 gcgccagtgg agcgcgatgc cggtgattgt gctttccgca cgcagcgaag agagcgacaa
     7981 aatcgccgcg ctggatgccg gagcggatga ttatctgagt aagccgtttg gcattggcga
     8041 attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc gccaccgccg cgcccgatcc
     8101 gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc cgcgtgattc accggggtga
     8161 ggaagaggtg catctcaccc cgattgaatt ccgcctactg gcggtgctgc tcaacaatgc
     8221 cggaaaagta ctcacccagc gccagctcct taaccaggtg tgggggccaa acgcggtcga
     8281 acacagtcac tatttgcgta tttatatggg acatctgcga caaaaactgg aacaggatcc
     8341 cgcccgccca cgccatttca ttactgaaac cggtattggc tatcggttta tgctttgaat
     8401 attaatttta atacagcctg cc
//
LOCUS       DAJSKJ010000167         8337 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000167 DAJSKJ010000000
VERSION     DAJSKJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.169"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(116..192)
                     /locus_tag="ON345_003252"
     tRNA            complement(116..192)
                     /locus_tag="ON345_003252"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Asp,seq:gtc)
     gene            complement(325..1056)
                     /gene="dnaQ"
                     /locus_tag="ON345_003253"
     CDS             complement(325..1056)
                     /gene="dnaQ"
                     /locus_tag="ON345_003253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR3073269.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1121..1588
                     /gene="rnhA"
                     /locus_tag="ON345_003254"
     CDS             1121..1588
                     /gene="rnhA"
                     /locus_tag="ON345_003254"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR3073270.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1585..2307)
                     /locus_tag="ON345_003255"
     CDS             complement(1585..2307)
                     /locus_tag="ON345_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3073271.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2341..3096
                     /gene="gloB"
                     /locus_tag="ON345_003256"
     CDS             2341..3096
                     /gene="gloB"
                     /locus_tag="ON345_003256"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR3073272.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3168..4526
                     /gene="mltD"
                     /locus_tag="ON345_003257"
     CDS             3168..4526
                     /gene="mltD"
                     /locus_tag="ON345_003257"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR3073273.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4574..5344)
                     /locus_tag="ON345_003258"
     CDS             complement(4574..5344)
                     /locus_tag="ON345_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3073274.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALVD
                     AIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5422..6222)
                     /locus_tag="ON345_003259"
     CDS             complement(5422..6222)
                     /locus_tag="ON345_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3073275.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6463..7377
                     /locus_tag="ON345_003260"
     CDS             6463..7377
                     /locus_tag="ON345_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3073276.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7374..8177)
                     /gene="dkgB"
                     /locus_tag="ON345_003261"
     CDS             complement(7374..8177)
                     /gene="dkgB"
                     /locus_tag="ON345_003261"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR3073277.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2230 a         2001 c         2026 g         2080 t
ORIGIN      
        1 acacatttac agcataacaa aaccatagac cgaagaaata gcgggattta aatgcaaaaa
       61 aaaccgcaaa attaaaaatc ttgcggttct ctgaactcat tttcatgagt gaatttggcg
      121 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
      181 aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc
      241 cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagccgct
      301 atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc ccttcttctc
      361 caccagatca agacgggctt catgagctgc aagctcttca tctgtcgcaa aaacaacgcg
      421 taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgctgtgt
      481 ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc
      541 cgcaaggatc tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc
      601 gtagcgggca cataacgcat cgaggctgtt gcgcttacca ggaaacattt tcctcgccac
      661 cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa
      721 cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc
      781 gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa
      841 aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt
      901 gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat
      961 ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt
     1021 atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttat
     1081 cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat
     1141 tttcaccgat ggttcgtgtc tgggcaatcc aggacctggg ggttacggcg ctattttacg
     1201 ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat
     1261 ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt
     1321 gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa
     1381 acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct
     1441 tgatgctgcc ctagggcaac atcaaatcaa atgggaatgg gttaaaggcc atgccggaca
     1501 cccggaaaac gaacgctgtg atgaactggc tcgtgctgcg gcaatgaatc ccacactgga
     1561 agatacgggc taccaagtcg aagtttaagc ttgtggttta cgacattgcc gggtggctcc
     1621 aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcgggttta gcgttaaagg
     1681 aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgcgcatt
     1741 caatagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgcagcac
     1801 ttcaaaattc aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctgtt
     1861 atagggcgag gtttttcgca ataccggcac aagtttgcgt aatcccatta aactaatggg
     1921 attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg tttcacgcaa
     1981 taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga
     2041 tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc
     2101 agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt
     2161 gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc
     2221 cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac
     2281 agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactaa gaggtaatct
     2341 atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa
     2401 gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc
     2461 gccaataact ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc
     2521 ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca
     2581 caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat
     2641 gaatttagtg taattgccac accgggtcac actttaggac atatctgtta cttcagtaaa
     2701 ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtaggtt gtttgaaggg
     2761 acagcatcac aaatgtatca atcacttaat aagttaagtg cgttacctga cgatacattg
     2821 gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg
     2881 cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc aaaaaatcaa
     2941 ataacactac ccgtaattct gaaaaatgag cggcaaatta atgttttttt aagaacggaa
     3001 gatattgatt taattaatgt aattaatgaa gaaacattat tgcaacaacc tgaagagcgt
     3061 tttgcatggt taaggtcaaa gaaagatagg ttctgataaa actttcttgt catcggctcc
     3121 gttcgccgtt atgatcggtc gtcttttaag caactattga cacacacatg aaggcaaaag
     3181 cgatattact cgcctctgtc ctgctcgtgg gttgccagag taccggcaac gttcaacagc
     3241 acgcacagag cctttctgca gctggtcaag gggaagcagc aaagtttaca agtcaggcac
     3301 gatggatgga cgatgggacg tctatcgcgc cagatggtga cttgtgggct ttcattggcg
     3361 acgagctaaa gatgggaatt ccggaaaatg accggattcg cgaacagaaa cagaaatatt
     3421 tacgcaataa gagctatctc cacgatgtaa ctttacgggc agagccgtat atgtactgga
     3481 tagcagggca agttaaaaaa cgtaacatgc ctatggaact ggtactacta cccatagtgg
     3541 agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc cgcaggcatc tggcagatca
     3601 ttccgagcac ggggcgcaat tatggtttga aacagacccg caattatgac gcgcgtcgcg
     3661 atgttgttgc ttcaacaact gccgcgctga atatgatgca gcgtctgaac aaaatgtttg
     3721 acggcgactg gcttctgacc gtagcggctt ataacagcgg cgaaggtcgg gtcatgaagg
     3781 caattaaaac gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta ccgttgccgc
     3841 aggaaacgaa gcagtacgtg cctaaaatgc tggcattgag tgatattctc aaaaacagca
     3901 agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg tgctctggcg cgtgtgcacc
     3961 tgagtagccc ggttgaaatg gcgaaggttg cagatatggc ggggatttcc gtcagcaagc
     4021 tgaagacatt caacgctggc gtgaaaggct ccacgctggg cgcaagtggg ccgcagtacg
     4081 tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc tctcgcttca ggcgaaattg
     4141 ctgctgtaca gtcgacgctg gttgccgaca atacgccgct taacagccgt gtttacaccg
     4201 tacgctctgg cgacacgctt tcaagcatcg cttcacgtct cggcgtaagc accaaagatt
     4261 tgcagcagtg gaacaaactg cgcggatcta agctgaagcc aggccaaagt ttgacgattg
     4321 gtgcaggcag tagcgcacag cggttggcaa acaacagcga tagcattacg tatcgtgtgc
     4381 gcaaaggcga ttcgctttca agcattgcta aacgccacgg cgtgaacatc aaagatgtga
     4441 tgcgctggaa cagcgacact gcgaatctgc aaccaggcga caagctgacg ttgtttgtga
     4501 aaaacaacaa catgccagat tcctgacaaa ccagataata aaaaggcacc gattcccccg
     4561 gtgccttttt tatttatgcc gctttatgtg cttctaccat gatgatatca ctggtgaaag
     4621 agccatcagt ctgcaaggaa aaatacgttc tcacctctgt cgatgcgctc tgttggtaaa
     4681 tgcgaatagc gtctactaac gcttctggcg tacgcattct cacgacccat gaagaaaatt
     4741 ccagcggtaa cttatctgta attaaattat caactatcag attggcttca ttgattaacg
     4801 tcaaccactc accgctggcg tagtttcgta cgtgagaggt atcgcgtaat gcttctaccg
     4861 tctgtaacca gatgtcgcgc actgggtggc ccggagacat tacgtccatc acaatcagcc
     4921 taccgccagg tttcaatatc cggttcactt cacgcagtgc tgcaccaaca tcatgccagt
     4981 gatgggcaga ataacggctg ataacaatat caaatgcgtt atcggcaaat ggcagacttt
     5041 cggcataacc ctggcgggta gtgatatttt tcagttgccg gacttcggca gcttgtgcca
     5101 cgacatccag catgtgggca gataagtcat acgccaccac cgtgctcacg ttttgcgcag
     5161 cgacaaagct ggcatgccct gctccgcagc ccatatcaag tacacttgca ccaggataat
     5221 cagccagacg caccgccagg cgctgtaaat ctcgtccgga tgcatgtacg gtactggtca
     5281 gatattcgca ggcttgagag ctaaactgct tttctacgtg gtcatggtgg gattgtgttg
     5341 tcattgcgct gtccttttgt tgtttgagta taaaaggaca gcgccaaacg ttggtctgcc
     5401 ctgtggcaga cctgacatac cttatttatc aggcttgccg ggactgaatt caacgagtag
     5461 cggattgtga tcggaagcgc gcgtaaccaa tacagaagct tcgctgacgt tcagaccacg
     5521 gtagaaaaca aaatcgagcg ggcggccaaa cgcccgacgg cgctgatcat cggtaaaacg
     5581 cacctggcgc agcgacattt cccgcgcaaa gcgatataac gcgttcatcc ttctacggct
     5641 ccaggcattg aaatctcccg ccataatgac cgggccgctg tggtgagcta tctgatcacc
     5701 aataggaagt aactgcttac tatagacatc cacgcccaga ctgaagttga cggcgtgtat
     5761 attaaccacc atcaacaggc gggtgtcagg taatggatag accgtcacca gtgccgactt
     5821 cgccagacgc aaaatgggtt ctcgttcacg taacgggcag caatacactg gatgcgccgc
     5881 cgaaagggtc attacaccgg aaggatgttg tggcagcacg aaagcgggca cctgatcggc
     5941 ggcaagatag ttagcggtcg caaactgtac taactctggc gttgtctgcg cttcctgcaa
     6001 taacaccaga tgtgcatctt tgccgtagtt ctttaatacc gacaaccatt cagcgcgttg
     6061 ctgtttgtat atgttccaca ccaggatccg aatacgctct tcggtactta acggttctcc
     6121 aggtggtaat gcctggccga tgctcgcaaa agaccccggc ggtaagatcc tttccgcagg
     6181 ttgtccggca acatagcgca tggcataggt gttttttcgc acttttgact ttgaaacctc
     6241 tattacgtca acccttccgc actcgggagg gttctactgt tatagggatt ttagctcact
     6301 ctttcaacga gcaattgtat attgttatgt aagcaagtgc ttacacaggt cagaattttg
     6361 ctcattattg agaacattaa atcggatgac atatcacgca tcctgatggt ttaatgacaa
     6421 ataaattcaa aatatctctt ctattttgca acaggagcaa aaatgaaagc cacgtcggaa
     6481 gaactcgcca tttttgtttc ggtcgtagaa agcggcagct ttagccgggc agcggaacaa
     6541 ttagggcaag caaactcagc ggtaagccgg gcagtgaaaa agctggagat gaaacttggc
     6601 gttagcctgc ttaatcggac cacgcgacaa cttagcctga cggaagaagg ccagcgttat
     6661 tttcgtcgcg tacagtcaat tttgcaggag atggcagcgg cagaatcaga aattatggag
     6721 acgcgtaata caccgcgtgg actgttgcgc atcgatgccg caactccagt ggtgctgcac
     6781 tttctgatgc cgttgattaa gcctttccgt gaacgctatc cggaagtcac tttatcgcta
     6841 gtctcctccg aaacgattat taatttgatc gaaagaaaag tggatgtcgc gatacgcgct
     6901 ggtacgttaa cggattccag cttacgtgcc aggccgttat ttaacagtta tcgaaaaatt
     6961 atcgcctccc ccgattatat ttcccgctac gggaagccag aaacgatcga cgatttaaag
     7021 caacatattt gcctgggatt cactgaaccc gcttccctca atacctggcc gatagcccgt
     7081 agcgatggac aattacatga ggtgaagtac ggtttgtcat ccaatagtgg ggaaacactg
     7141 aaacagcttt gcctgagtgg caacgggatt gcgtgtttgt ccgactacat gatcgacaaa
     7201 gaaatcgctc gcggagaatt ggtggagtta atggcagata aagtgttgcc agtggaaatg
     7261 ccattcagtg ccgtctatta cagcgaccgt gcggtaagta cgcgcatccg ggcttttatc
     7321 gatttcctta gcgagcatgt aaaaacagct cccggaggag ctgtcagaga ggcttaatcc
     7381 cattcaggag ccagaccttc cgggctaacc aggcggtcgt tgcaatccag tgcggcgatc
     7441 gcttttttat cttcggcatc aagctgtaaa ttttgtgcct taagattact ttccaggttt
     7501 ttacgtttag tagaagaagg aattactgag taaccttccc ccatagccca cgccagaatc
     7561 acttgtgccg gagtcgcatt gtgtttagct gcgatacgag caataacctc atctttcagg
     7621 gccttaccat acgccagcgt catataggaa gtaatatgga tgccgtgctg tttagcccag
     7681 gcaaccactt tacggttttg cagataagga gagagttcaa tctggttagt agcgatgttt
     7741 tcagcaccaa cagcagcaat cgctttttcc atcaacggga tcgtgaagtt ggaaataccg
     7801 atctcacgcg tcagcccttg ttttttggct tccagcagcg cctgcataaa ctcttcaaca
     7861 gagacttcat cgtttggtga cggccagtgg attagcgtca gatcaacata atcggtacgc
     7921 aatttttgca ggctctcttt cagacttggg atcaatttgt ctttgctgag attttcaatc
     7981 cagattttag tggtgatgta gagttcatga cgtggcacgc cactttctgc aatcgcctga
     8041 cctactgcgg cttcgttatc atagatttgt gcggtatcaa ttgcgcgata accaagttca
     8101 agcgccgtta tcacagatga aataacaacg tcgtctttca gacggaaagt acctaaacca
     8161 aatgcaggga tagccataat atgcctcttt taatgctatt ttcgttaact gaggtgatta
     8221 tgggcttcag actgttgagg aaaaagagcg taaaatgcag aggatttttg cgattctggc
     8281 aataatagat atacagacga aaaaaaacct cgagcgttaa ctcgaggctt cttaata
//
LOCUS       DAJSKJ010000168         8152 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000168 DAJSKJ010000000
VERSION     DAJSKJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.170"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..575)
                     /locus_tag="ON345_003262"
     CDS             complement(<1..575)
                     /locus_tag="ON345_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3073278.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITL"
     gene            complement(777..1481)
                     /locus_tag="ON345_003263"
     CDS             complement(777..1481)
                     /locus_tag="ON345_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3073279.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1618..2070)
                     /gene="moaE"
                     /locus_tag="ON345_003264"
     CDS             complement(1618..2070)
                     /gene="moaE"
                     /locus_tag="ON345_003264"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR3073280.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(2072..2317)
                     /gene="moaD"
                     /locus_tag="ON345_003265"
     CDS             complement(2072..2317)
                     /gene="moaD"
                     /locus_tag="ON345_003265"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR3073281.1"
                     /translation="MIKVLFFAQVRELVGTNATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2310..2795)
                     /gene="moaC"
                     /locus_tag="ON345_003266"
     CDS             complement(2310..2795)
                     /gene="moaC"
                     /locus_tag="ON345_003266"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR3073282.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2798..3310)
                     /gene="moaB"
                     /locus_tag="ON345_003267"
     CDS             complement(2798..3310)
                     /gene="moaB"
                     /locus_tag="ON345_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR3073283.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3332..4321)
                     /gene="moaA"
                     /locus_tag="ON345_003268"
     CDS             complement(3332..4321)
                     /gene="moaA"
                     /locus_tag="ON345_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR3073284.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4309..4453)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4718..5626
                     /gene="yvcK"
                     /locus_tag="ON345_003269"
     CDS             4718..5626
                     /gene="yvcK"
                     /locus_tag="ON345_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR3073285.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5818..7839)
                     /gene="uvrB"
                     /locus_tag="ON345_003270"
     CDS             complement(5818..7839)
                     /gene="uvrB"
                     /locus_tag="ON345_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR3073286.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         2017 a         2147 c         2027 g         1961 t
ORIGIN      
        1 tagagtgtta ttgccccagg aacgatacag cgaagagaac ctgaaatttc agaggtgtcg
       61 gcaacacgga tcaaatcttc cagtgttgtt gcttcacagg cagccgtaat gccccagata
      121 aagacagtca aaatactcgc taaccacacg gaaaaactgc tggtaaggat caggttaaca
      181 acaaaagcaa tactcaaacc acagaaaata tatgaatata actgtctgac tggtgggata
      241 accctattac tacaagagca aatcagcgcc attgcggcaa acatccctcc cgtaattccc
      301 attaccgtcg ctatgctcga aaaggtataa aggctggcga agagaaagcc aacaagaagg
      361 attattagca gatagaccag acatgcgcct gatacattca gtcttttcag ggcgttgttt
      421 aaccagagac cacctattat cagcgcagcc aaagcgaaga caaacgcacc tggagacttt
      481 tgtgacgtaa cccaatgcaa atgctgaaat atccaggcac agcctgccgt agcccatact
      541 ccagctgctg ttaaccacca aatgagaagc agcattttgc tgagcgaagc acttttttgc
      601 tgtaattgag tagcagtgga ttgtctgttc aaatgcgtta tctgtccctg aaattacgta
      661 gtggacatag tctggcattt gccgcgattc agttctcatg taaatgagcg gaatagtctt
      721 tataaaatcc gaaaaaaatc ccggctccag gccgggatca gcaggaagaa gagaatttaa
      781 cggcggttgc cgaagatccg caacaacatc aggaacaggt tgatgaagtc cagatacaag
      841 gttaacgcgc caagaatgga atatttgcgc aggttcgacg tgtcgcgggt atcaatctgc
      901 tcacccatat ttttcagttt ctgcgtgtca tacgccgtca atccgacaaa gacaatcacg
      961 ccgatgtagg taactgccca catcaatgct tcgcttttca accagaagtt gaccagcgat
     1021 gccagcacaa tgccgattaa cgccataaac agcatattgc caaagccact taaatcgcgc
     1081 ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc cggcagtaac gacgaaagta
     1141 ctggcgatag aagcagcggt atagacaatg aatatactgg aaagcgtaag acccgtcagc
     1201 gccgaataaa gcataaagag catcgtcgtt acacctgcgc tcagcttttg aatcatcgct
     1261 gataacacaa taaccaatgc taattgcgcg atgatcagac cgattaaaaa gacacggtta
     1321 gtgaacaaca gctccatcac ggccgcggaa ttagccgcat accaggcaac aaatgcggtc
     1381 agcaacaagc caacggtcat ccagccatag acttgcgcca tataagtttg caagccagcc
     1441 cggggttgta cgattgaatc agaacgtggg aatctgtcca tgacgatctc ctgaagatat
     1501 aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgt
     1561 atgtactgag cggtgaaatt gccggacgca gcggtgcctt atccggctaa caaaaaacta
     1621 ccagcgtttt gccgcctgct gatcgctctc ccgagcttca acccagcggt cgccttccgg
     1681 cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa taaactgccc
     1741 ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg acaaaaacga tttcatcgcc
     1801 cggccataat tccccgatgc ggtgaatcac agtgacgcgc cccagcggcc agcggttacg
     1861 cgcttcatca acaatttctg ccagtgcttt ttcagtcatc cccggatagt gttcgagggt
     1921 taatgctttg acgctgtcgc ccaggttatg gttgcgcacc ttaccagtaa aggtgactac
     1981 cgcaccgtct tcgtcacgct ccgccagcca cgggtactct tctcctacgc tgaacggctg
     2041 cggaccaaca acaattttgg tttctgccat cttaacctcc ggttaccggc gggaagaaag
     2101 ctacttcgtc gccgtcagtc agcggatggt caaaactcac cagcgtctgg ttgacggcag
     2161 ccagtaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca gccatgtgct
     2221 ggcgtaacgc ttcaacagtt gggaaatccg cagccacttc ggttgcattt gttcccacca
     2281 actcgcgcac ctgggcgaaa aaaagaactt taatcatccg cttccacctt aaagtcaccc
     2341 gacttgccgc cgctcttcgc cagcaaacgt accggaccaa tcaccatatc tttttgcacc
     2401 gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa tgcttccatt
     2461 tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgcacccg attgtgctcc
     2521 ggctcggcct gtaaattgac ttcaactttg ctgagcatca gcggatgaca gagcgggatc
     2581 agatcccagg tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc aaatacgtcg
     2641 cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat ggtgacaaag
     2701 gcttccgccc gcgcttcacg cacggtttcc gctttggcgg agacatccac catgtgcgct
     2761 tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa tgtggatgga
     2821 aattacacgg acgcgtacgg gcatccagct gcggcgcgat gatattttcc catgcggtac
     2881 ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttggcg acgcccgcta
     2941 ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacatc cggaacactt
     3001 caccaaaacc ttcaacttca cggtcgaaca acggcagcaa tgcttcggga gcctgatcac
     3061 cttccgtcag gccagtacca cccgtaatca ataccacttg tacatcgtcg ctggcgatcc
     3121 acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct ttatcgacaa
     3181 cgtgatggcc cgcttcttgc gccgaatcgc gcagatagtg accggaggta tcgtcttctt
     3241 caccgcgacg attagaaacc gtaagaatag caatacgggt cgggataaat tcagtgctta
     3301 cctgactcat ctgatctctc cttttgacgt tttagccgcc aatgtacgat aagttttgcg
     3361 taataccggt gttgttttga tgcaggaaat gggtctgttt cttctcccgc agcgccgctg
     3421 aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa
     3481 cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg cgcaggcggt
     3541 tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct ccggcgtaat
     3601 ctggatggca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg tggatccagc
     3661 cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta cggaagagct
     3721 cgctgccctc gcccgtttcc atcagttcga tgaaacgcag ctggataggg cgatgctgga
     3781 tccagttcag aaaggtgtcg agctggtgat gattaacatc acgcatcagc acggtattga
     3841 ctttgacctt ctcaaaaccg gcctcaaatg cagcatcaat ccccgccatg acctggttga
     3901 atttatcctg cccggtaata gcgtgaaact ggcgggcgtc cagactgtcg acactgacgt
     3961 taatgccagt aagtcccgca tcgcgccagt tcgccacatc gcgttccaga cggtaaccat
     4021 tggtggtgac cgcaatctgg cggatagcgt cgttttcccg cacagcggcg atgatatcgg
     4081 taaagtcgcg gcgtaaagac ggctcaccgc ctgtcaggcg cactttttcg gtgcccagac
     4141 tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt aagaaagcct ttattggtga
     4201 cgccgctcgg tttgtagcca tccggcaggc agtaggtgca acgaaagtta cacacatcgg
     4261 taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca
     4321 tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg gtggcaatct
     4381 ttacgagcat tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag aggctagagt
     4441 gtttagtggt tatgccgata ctagcgtgta aatgagattt ttaccatcca cactttcgct
     4501 atgtaatcat gtatatagcg tcatgatcgt gctatttcgc tacagaataa gggaaaatgg
     4561 cggttttact ttgatatcca tcatcaggga ggcatagcgt gcatcatcct ttagaggtag
     4621 aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcccc gcaatttgcg
     4681 ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta cgctggctga
     4741 tcttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc tctcatcact
     4801 ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc accgataatg gtggctcgac
     4861 ggggcgtatt cgccgttcag aaggcggcat tgcctggggc gatatgcgca actgcctcaa
     4921 ccagctgata acggaaccga gcgtcgcctc cgcgatgttt gaataccgtt ttggtggcaa
     4981 tggcgaactt tccggtcata atctcggaaa cttgatgtta aaggcgctgg atcaccttag
     5041 cgtgcggcct ctggaagcca tcaatttaat tcgtaatctg ctgaaagtgg atacgcattt
     5101 gattccaatg tcagagcatc ctgttgatct gatggcgatt gacgatcagg ggcatgaagt
     5161 ttacggcgag gtcaatatcg accagttaac tacgccgatt caagagttat tgttaacgcc
     5221 taatgtaccc gcaacgcgtg aggcggttca cgctatcaat gaagcggatc tcatcattat
     5281 tgggcctggc agtttttata ccagcctgat gccaattctg ctgctgaagg aaatcgccca
     5341 ggcattacgc cgcacgccag cgccgatggt ttatatcggc aatctggggc gtgagttgag
     5401 tttacctgcg gctaatttga agctggaaag caagctggca attatggagc agtatgttgg
     5461 taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg cggtgaaaga
     5521 gcggattgtg atccaggagg tactggaggc cagcgatatt ccgtatcgtc atgaccgcca
     5581 gttgttacat aacgcgctgg aaaaggcgtt acaggcttta ggttaacgga aatccgaagg
     5641 aaaattccgg cttcctattg aagacaaagt gcgcgttgtt tatgccggat gcggcgtgaa
     5701 cgccttatcc ggcctacaaa ccgcgcaaat tcaatatatt gcggagaaaa tgtaggcctg
     5761 ataagcgtag cgcatcaggc tgttttccgt ttgtcatcag tcttcttcgc tatcctgtta
     5821 cgatgccgcg ataaacagct cacgcagctg atgcaactgg tcacgaattt gcgccgcttc
     5881 ttcgaactcc agattctgcg cgtgttgcat catcaaccct tccagctcat ggattttctg
     5941 ctgcaacgct ttaggcgaca tatccatcgg cacattatcc ggctcaacaa tcgggcgcga
     6001 ttttcctctg cccttcgctt tggttttggc aatgttctgc cccagcgcca ggatatcgac
     6061 cactttcttg ttcaagcctt gcggcgtaat gccgtgttcc tcgttgtact tctgctgttt
     6121 ctcacggcga cgttcggttt cgccaatcgc tttcgccatt gatggggtga tcttatcgcc
     6181 gtagagaatc gctttaccgt taacgttacg tgccgcacga ccaatggtct ggatcaacga
     6241 acgttcggaa cgcaggaagc cttctttgtc agcgtcgagg atcgccacca gcgacacttc
     6301 cggcatatcc agaccttcgc gcagtaagtt gatccctacc agcacgtcga actcacccag
     6361 acgcaagtcg cggataatct ccatacgttc gacggtgtcg atatctgagt gaagataacg
     6421 cacgcgctcg ccatgttctt cgagatattc ggtaagatct tccgccatcc gcttggtcag
     6481 tgtggtgacc agtacgcgtt cgttaattgc cgctcgctga cgaatctccg aaagaagatc
     6541 atcaacctgt gtcgccaccg gccgcacttc gataatcggg tcaagcaatc cggttggacg
     6601 caccacctga tccaccacat cgccgccgga tttttccagc tcgtaattac ccggcgtcgc
     6661 cgaaacatag atggtttgcg gcgctaatgc ttcgaactct tcaaacttaa gcggacggtt
     6721 atccagcgct gatggcaggc ggaagccgta ctccaccagt gtctctttac gcgcccggtc
     6781 accgcgatac atgccgccaa tttgtggaat ggtgacgtga gattcatcga cgaccagcag
     6841 cccatcggca ggcaggtaat caaacagcgt cggcggtggc tcacccggtc cacgaccgga
     6901 gaggaagcgc gagtagtttt caatccccga acagtagccc agctcgttca tcatctccag
     6961 atcaaactgg gtacgctggg tcagccgctg ctcttccagc agtttgttgt tttccaacag
     7021 cactttgcgt ctggcggcca gttcttcttt gatctcctcc atcgcctgta cgatgcgctc
     7081 gcgcggtgtg acgtagtgcg ttttcgggta gatggtaaaa cgtggaatag tggaaacaat
     7141 ctgcccggtc agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa acagttccac
     7201 gcgaagtgca atgtcatccg attctgccgg gaagatatca atcacctcgc cacgaacgcg
     7261 gaaagtacca cgctggaatg cttgatcatt acgagcgtat tgcagctccg ccagtcggcg
     7321 cagaatcgcg cgctgatcga taatcatacc gaccgtgaga tggagcatca tcttgagata
     7381 taaatcagga tcgcccagac cataaatcgc ggaaacagag gccactacaa ccacatcacg
     7441 ccgctccagc atcgctttgg tggcggataa acgcatctgc tcaatatgtt cgttaaccga
     7501 ggcatctttc tcaatgaaag tgtcggaact cggtacatag gcttccggct gatagtagtc
     7561 gtagtaggag acgaaatatt ccaccgcgtt ttccgggaag aactctttca tttcgccata
     7621 cagctgggcc gccagcgttt tgttgggcgc aagtaccatg gtagggcgct gaaggtcagc
     7681 aatgacattg gcaatggtga atgttttccc ggagccagtc acgccaagta acgtctggtg
     7741 cgccaggcca tcttccagcc cctcttcgag acgtcgaatc gcctctggct gatcgccaga
     7801 aggtttaaaa gcggaattca atttgaacgg tttactcatg agtcgctacc tgaaggagtt
     7861 gggcgggcag gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa ttatactgga
     7921 taaaaaaaca gttcatcacc ataatatttc tgttacaacg taaactccgc tgtcaatcat
     7981 gagcaagatt tactctgtgg cgagataaaa ctccggcctt accgggttat ccccaaagca
     8041 acggcttttt taacatttgt caagatgagt gatgacagtt ttatggcaag agatgcctgt
     8101 tcagtgactg ccattgcaat tatctaacca attaaaaaat aaaagatatt tc
//
LOCUS       DAJSKJ010000169         8107 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000169 DAJSKJ010000000
VERSION     DAJSKJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.171"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(170..2131)
                     /gene="topB"
                     /locus_tag="ON345_003271"
     CDS             complement(170..2131)
                     /gene="topB"
                     /locus_tag="ON345_003271"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3073287.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPMVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERKTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2136..3179)
                     /gene="selD"
                     /locus_tag="ON345_003272"
     CDS             complement(2136..3179)
                     /gene="selD"
                     /locus_tag="ON345_003272"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR3073288.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3296..3847)
                     /locus_tag="ON345_003273"
     CDS             complement(3296..3847)
                     /locus_tag="ON345_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR3073289.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREVFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4008..5864
                     /gene="sppA"
                     /locus_tag="ON345_003274"
     CDS             4008..5864
                     /gene="sppA"
                     /locus_tag="ON345_003274"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3073290.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6031..7047
                     /gene="ansA"
                     /locus_tag="ON345_003275"
     CDS             6031..7047
                     /gene="ansA"
                     /locus_tag="ON345_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR3073291.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7058..7699
                     /gene="pncA"
                     /locus_tag="ON345_003276"
     CDS             7058..7699
                     /gene="pncA"
                     /locus_tag="ON345_003276"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR3073292.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7792..>8107)
                     /locus_tag="ON345_003277"
     CDS             complement(7792..>8107)
                     /locus_tag="ON345_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073293.1"
                     /translation="EWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
BASE COUNT         1901 a         2195 c         2055 g         1956 t
ORIGIN      
        1 cttctcgctc ccaggatggt tttgtaataa aacttttttc atcacattcc ctgttattac
       61 atactttaga ggcgataatt ttatccatgc aaaaaaaata tccaacgaat tttcttgatc
      121 cgctggatat ttcagaatat gactcgaata gcacgaaaga ttcgctcgct tacgctatcg
      181 ccccgcttcc gacttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag
      241 cctttttctt atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca
      301 ccggcgtacg tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa
      361 agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg
      421 tcatgtccgg tcgcgtcgcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg
      481 tggagtggat atagcgccct tttttggtca gaaaaccacg cttgaacagc agttcaataa
      541 tcccggcacg ggttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat
      601 ctttgtcctg cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt gcatcggtaa
      661 aatggcgcgg cggctgggtt ttccgctcta ccacttcacc tttttcacac agcaactcat
      721 cgcctttcgc caccacaggc aatggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc
      781 ctaacagcgt gcgccagcct gcttcagcaa gaaaacgggc tttagcgaca aatttgcctt
      841 tggcaatgtc cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca
      901 ggtattgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag
      961 aactccgtgc ggtcgggatg atggcatggt gcgcatcgac ctttttgtca tcccaacagc
     1021 ggttgcgtat atctggatct accattggct gcggcaacag atctggtgca tgaacgctga
     1081 tggcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg
     1141 aacgcggata agtgattagc ttgtgcgttt cgtacagctt ctggcagata tcaagcacgt
     1201 tctgcgcgct cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaaag
     1261 gcagcggcgc ggattctgat tcccgtttat cgttatagct ggtgacaatt gctggttgac
     1321 cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat
     1381 cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag
     1441 gtgtcacgat atgtgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt
     1501 catcgcggcg caccaccagc ccgagcacgg gtgtctgcac gcgtcccacg gaaagtacgc
     1561 cctgataacc ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca
     1621 gccagtcggc acgcgctcgc gccagcgcag aaacgcacaa tggcacaaac tcactgttgg
     1681 accgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac
     1741 gctgcacctg ctggcgcttt tccggtgcca gttgcagata gtccagcact tcatccacca
     1801 gcagttgccc ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcagga
     1861 agcgtttgat gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt
     1921 ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg
     1981 gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact
     2041 cgataaagcc atcgccttta cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca
     2101 gactcggttt ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga
     2161 ccgccgcgcg ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct
     2221 gtagctttaa cctcattttc tgcttccggc atgaccgcca gcagcaaacc gccagaagtt
     2281 tgcggatcgc acagcaaatc gcgcacttca cgcggcattt cacccatcag atgaccgtag
     2341 ctggcaaagt tacgttcggt gccgccaggc actgcgccca acttaatgta ctcttcaacc
     2401 cccggcaatt ttgggatcgc gtcatagtca acgcgcgcct gcacacctgc gccctgacac
     2461 atttcgctca aatggcccag cagaccaaaa cccgtaacgt cggtcatcgc ttttacgcct
     2521 tcgatgttgg caaaggacgc gcctgcgatg ttcatccggc acatcacttc cgtcgctagt
     2581 ccctgatgtt ctggtttcag cagtgatttt ttctcagccg tggtaagaac gccgatcccc
     2641 agcggtttcg tcaggaacag tttgcatccg gcttgtgcgg tactgttttt cttcacccgc
     2701 tcggtcggta cgatccccgt taccgccaga ccaaaaatcg gctccggcgc atcgatggag
     2761 tgaccgccag ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggttact
     2821 tcgcgggcaa tttccgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata
     2881 atcggtttgc cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca
     2941 aaatcgaaag gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg
     3001 ccattgccca gatcgtacac cgccgcatcg tcgcgggtct cattacccac aagcaaattc
     3061 ggatcaacaa acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt
     3121 ttacagccgc aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg
     3181 gacatctcct gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga
     3241 gagtgtctga attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga
     3301 aataagttac aaacggcgtc gggtccggga cgttaatcga cgtagatgct ttcaactgcg
     3361 gcgtgccgag gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatacttcac
     3421 gcactaccgg actttcagtt aatgcgccac tgcgccagat gccgccaaat ccctgggcaa
     3481 cggctgccat ttgcatcgcc atgaccgcgc atccggcaga catttcctgt tcccatcgcg
     3541 ggactttatg attttcttcg catttcgcca ccaccgtgat gatgagcggt gcgcggaacg
     3601 gcgcattacg ggctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt
     3661 ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa tgccacggtt
     3721 gcatggactt atggtccggc gcacgcatac ccgcacgcag gatgttttgc agttgttcac
     3781 ccgttggcgc gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg
     3841 catccatttg attaactcct gtcgtgatat ttattcacaa aattaacacg agagtggatt
     3901 ttgttacagc acagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc
     3961 acatctattg cgcctgtgac aggtgtgacc ttaagttggg agaatacatg cgaacccttt
     4021 ggcgatttat tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa
     4081 tggtacttaa cctgttcttt attttcctcg tactggttgg tgtggggatc tggatgcagg
     4141 tcagtggtgg tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg gacatttctg
     4201 gtgtgatcgt cgataaaccc gacagttctc agcggtttag taaattaagc cgccagctgc
     4261 ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc
     4321 gccaggcgaa ggacgaccgc aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag
     4381 gcggcgacca gccgtctatg cagtacatcg gcaaagccct gaaagagttt cgtgacagcg
     4441 ggaaaccggt ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt
     4501 tcgccaataa aatttggctg tcaccgcaag gcgtggtgga tttgcacggt tttgctacca
     4561 acggtctgta ctacaaatcg ttgctggata agctgaaagt ttccacccat gtgttccgcg
     4621 tgggtacgta taaatctgcc gttgaaccgt ttattcgtga tgacatgtca ccggcagccc
     4681 gcgaagctga cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg
     4741 ctaaccggca gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt
     4801 taaccaaaac cggtggcgat accgcgaaat atgcactgga aaacaagctg gtcgatgcac
     4861 tggcatcgag tgccgaaatc gaaaaagctc tgaccaaaga gttcggctgg agtaagactg
     4921 ataaaaatta tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg
     4981 gtgacagcat cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg
     5041 ggaatgttgg cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag
     5101 tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga
     5161 ttcgcgctga actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg
     5221 gcatggcggc atctggtggt tactggattt ccacgccagc taattacatt gtggctaacc
     5281 ccagcaccct gaccggttct atcggtatct tcggcgtgat caccaccgta gaaaatagtc
     5341 tggattcgat tggtgttcat actgatggtg tctcaacttc accgctggcg gatgtttcta
     5401 tcaccagggc actgccgccg gaagcgcagc agatgatgca attaagcatt gagaatggct
     5461 ataaacgctt tatcacgctg gttgctgatg cgcgtcattc gacgccggag caaattgata
     5521 aaatcgccca gggccacgtc tggaccggtc aggatgcaaa agctaacggg ctggtcgata
     5581 gtctcgggga tttcgatgat gcggttgcca aagcagcaga gctcgcaaaa gtgaaacagt
     5641 ggcatctgga atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt
     5701 ctggttctgt ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgcttg
     5761 cctcggtagc ctctaccgtt aaaagtgaaa gcgacaagct ggccgcgttt aacgacccac
     5821 aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg tactgagtgg
     5881 ccgacagatc gtcggccaca ttatttttta cctcgacgaa tcctcttccc gctgtctcgc
     5941 cccatatttc cttatccgca tagtatcagg tgcgctcctc ctgcctcacg tatatacttt
     6001 tgctctttcg atatcattca tatcaatatc atgcaaaaga aatcaattta cgtcgcctac
     6061 acgggcggga ccatcgggat gcagcgttcc gagcagggtt atattccggt gtcaggtcat
     6121 ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc agatttcacc
     6181 attcatgaat atacgccgtt gatggattct tctgatatga cgccggaaga ctggcagcat
     6241 attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat tctacacggt
     6301 accgacacga tggcgtatac cgcctctgcg ctgtcgttca tgctcgagaa tctcggtaaa
     6361 ccggtcattg tgacagggtc acaaatcccg ctggctgagt tacgctctga cggacaaatt
     6421 aatctgctga atgcgttgta cgttgcggcg aattatccga tcaacgaagt aacgctcttt
     6481 ttcaataacc gattgtatcg cggcaaccgt actaccaaag cccatgccga tggttttgat
     6541 gcgtttgcct ctccaaacct tcctccgtta ctggaagctg gtatccatat tcgtcgtttg
     6601 aatacgccac ccgccccgca cggtgaaggg gaattaatcg ttcatccaat cacgccacaa
     6661 ccaattggcg tagtgacgat ttatccaggg atttctgctg acgtcgtgcg caattttctg
     6721 cgccaaccgg tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc gccacaaaac
     6781 aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt ggtcaacctg
     6841 acacaatgta tgtccggtaa agtgaatatg ggtggttatg ccaccggtaa cgccctcgcc
     6901 catgccggcg ttattggcgg tgcagatatg actgtagaag ccacgctaac caaactgcat
     6961 tacctgctga gccaggaact ggatactgaa accattcgca aggccatgag ccaaaacctg
     7021 cgcggcgaac tgacgccgga tgattaagga gactgcaatg ccccatcgcg ccctgttact
     7081 ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtaccgg aaggtgacag
     7141 tacggtggag gttgctaacc gccttattga ctggtgccag tcgcgcggag aagcggttat
     7201 cgccagccag gactggcatc cggcgaatca cggcagtttt gccagtcagc acggtgtaga
     7261 gccttatacg ccaggccaac tcgacggttt gccacaaacc ttctggccag atcactgtgt
     7321 gcagaacagt gaaggcgcac aattacatcc gttactgaac caaaaagcga tcgcagcggt
     7381 gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg ataacggccg
     7441 tcggcagaaa acctctctcg atgactggtt acgcgatcat gaaatcgatg aattgatcgt
     7501 tatgggcctg gctactgact attgcgtgaa gtttaccgtg ctggacgcgt tacagttagg
     7561 ttataaggtg aacgtgatta ccgatggttg tcgtggcgtg aatatccagc cccaggacag
     7621 tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg ctatatacgc tggcagactg
     7681 ggaagagacg caggggtaat tttacgctgg cctacaattc tgtactggca ttgtaggcca
     7741 aataaaacac gtcagtggca catctggcaa ttgatgccat caacgaaaga tttaattcac
     7801 ctcagaaatc tcttccaacg acaccttccg cgtttcgatg ccaaaaatgg agagaaccag
     7861 cgcacaaagc aataacatga caccaagtac cataaacacc gtgatcgacc cataatgtgt
     7921 taataatgcc gcaacgccat agggcgtgaa aactgcgacg atccgtccga cggcattaac
     7981 gaaacccgaa ccgcgcaggc gtaaatgcgt tggccaaagc tccgggatat aaaccgccga
     8041 cgcgaagcaa acatacatgt ataaaaagaa gatcatcacc aggccataga ttaaaatcgc
     8101 ccactct
//
LOCUS       DAJSKJ010000170         8046 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000170 DAJSKJ010000000
VERSION     DAJSKJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.172"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            361..858
                     /gene="ubiC"
                     /locus_tag="ON345_003278"
     CDS             361..858
                     /gene="ubiC"
                     /locus_tag="ON345_003278"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR3073294.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     MVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            871..1743
                     /gene="ubiA"
                     /locus_tag="ON345_003279"
     CDS             871..1743
                     /gene="ubiA"
                     /locus_tag="ON345_003279"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR3073295.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1898..4321)
                     /gene="plsB"
                     /locus_tag="ON345_003280"
     CDS             complement(1898..4321)
                     /gene="plsB"
                     /locus_tag="ON345_003280"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR3073296.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEE"
     gene            4492..4860
                     /gene="dgkA"
                     /locus_tag="ON345_003281"
     CDS             4492..4860
                     /gene="dgkA"
                     /locus_tag="ON345_003281"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3073297.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            4970..5578
                     /gene="lexA"
                     /locus_tag="ON345_003282"
     CDS             4970..5578
                     /gene="lexA"
                     /locus_tag="ON345_003282"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR3073298.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5651..6976
                     /gene="dinF"
                     /locus_tag="ON345_003283"
     CDS             5651..6976
                     /gene="dinF"
                     /locus_tag="ON345_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR3073299.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAM"
     gene            7092..7301
                     /locus_tag="ON345_003284"
     CDS             7092..7301
                     /locus_tag="ON345_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3073300.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7343..7858)
                     /gene="zur"
                     /locus_tag="ON345_003285"
     CDS             complement(7343..7858)
                     /gene="zur"
                     /locus_tag="ON345_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR3073301.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1815 a         1968 c         2207 g         2056 t
ORIGIN      
        1 aaatccagac ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa
       61 tctcatggta aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata
      121 aagaatatag gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat
      181 gcccctacgg ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcggtt
      241 taccacttca tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca
      301 tctcccgtaa acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc
      361 atgtcacacc ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc
      421 ctggatccgc aactgctcga ctggctgttg ctggaggatt ccatgacaaa acgttttgaa
      481 cagcagggaa aaatggtaag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa
      541 atccccgaag aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg
      601 ttatgtgccg atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta
      661 agcgggccgg agctggcgtt acaaaaattg ggtaaaacgc cgttaggacg ctatctgttc
      721 acatcatcga cattaacccg ggactttatt gagataggcc gtgatgccgg gctgtggggg
      781 cgacgttccc gcctgcgatt aagcggtaaa ccgctgttgt taacagaact gtttttaccg
      841 gcgtcaccgt tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg
      901 ctggcgtttc atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg
      961 ccaacattat gggcgttgtg ggtggcgaca ccgggcgttc ctcagctctg gatcctggcg
     1021 gtatttgtcg cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct
     1081 gaccgaaagt ttgatggtca tgttaagcgc acggcgaacc gaccacttcc cagcggcgcg
     1141 gtaacagaga aagaggcgcg cgcgctgttt gtagtgctgg tactgatttc gtttttactg
     1201 gtactgacgc tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg
     1261 gtgtacccgt ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt
     1321 ggctggtcga ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg
     1381 ttaatgttcc tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcgatggtt
     1441 gaccgcgatg atgatgtgaa gattggcatt aaatccacgg caatcctgtt cggccaatac
     1501 gataaattga ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt
     1561 gagttaaatg gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt
     1621 gtttatcaac aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg
     1681 aataataact atgttggtct ggtactattt ttagggctgg caatgagtta ctggcatttc
     1741 tgatgatgta aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgcggc
     1801 gcttctcagg cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg
     1861 ataaggcgtt ttcgccgcat ccggcaattc tctctgatta ctcttcgccc tgcgtcgcac
     1921 tctcaatcgt caaacgcacg tctgatgtaa tcaactccgc cagcaattga taaaccttca
     1981 tcgtttctgc cggttcggca tcgccgctat cgctgatata cccttcatca cgcagcgtca
     2041 gcaccagaga actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca
     2101 cggagagacg ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg
     2161 ggttggcact caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg
     2221 cggccagcag ctgtagggtg cgagaatgcg ccgggttgat atgcaactca tcatcttgca
     2281 gggtaatcag cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact
     2341 cgtcgcgatc ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt
     2401 gctccatcaa tacgtcgcgg gagatgtggc gatgctgggt gacgattgcc gccatcagcg
     2461 aaggcagcac caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct
     2521 cgcgcggcag aatgatgatg tcgccgattg tgtctttctc gacttcaaac ttgttcattt
     2581 gcagcgcgtg atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt
     2641 aaggtacgtt gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg
     2701 tgagtgagcg ctgacgtgac gccagaagcg cagtacagca caggttcatg gcgtttgccg
     2761 cgcctgcgtt gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta
     2821 accatgccgg acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct
     2881 ggttaaggta ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat
     2941 tacgcagctt gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc
     3001 cgcgcagttc tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg
     3061 gaatcagcgt aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg
     3121 tgccggtttt cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact
     3181 cgacggaata accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa
     3241 gtttattgcc tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc
     3301 cggccggcca gaagttcagg ttgatcccag cggcgatatg cggcggcacc agcccctggt
     3361 gatacagcac gtaagaaagc agcaggtagt ccatgtggct gcggtggcaa ggcacatata
     3421 ccagctcatg accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc
     3481 cctgataaag tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt
     3541 aagagaaatt tgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat
     3601 gggagatttt tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga
     3661 gcagcttatt aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt
     3721 gacgggcaaa gtgcatacgc gccacgcgcg ccagtttctg agcgatagtt ttatctgtgc
     3781 cgtgttcatc cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc
     3841 gaccgagcca caatacagcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt
     3901 tcacttcgcc tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct
     3961 gcacatccag atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag
     4021 actcttcttt cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc
     4081 gcggcagtag cgtgccgtcg atttccagcg gctctaacgg gtcaggcaaa tcatgtgcca
     4141 gacactgggc gcgcaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa
     4201 ttggacgaga ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt
     4261 ttaccaggat gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca
     4321 taaacgatgt aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca
     4381 tgggaaattc tgtggtatcc gctcatgttt cgcacggcgt tacgcaaacc cgaatcatcg
     4441 gatttaacgg tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat
     4501 aataccactg gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc
     4561 gctgcatgga tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg
     4621 gtcatcgcct gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg
     4681 atgctggtga tgattgtgga aatcctcaat agcgccatcg aagcagtggt tgaccgaatt
     4741 ggctctgaat accatgagct ttccggacgc gcaaaagata tgggatccgc cgcggtgctg
     4801 attgccatta tcgtcgccgt gattacctgg tgcatcctgt tatggtcgca ttttggataa
     4861 cccttccaga tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct
     4921 tttgctgtat atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt
     4981 aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat
     5041 gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga
     5101 agaacatctg aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg
     5161 cgggattcgt ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc
     5221 cggtgaaccg cttctggcgc aacagcatat tgaaggtcat tatcaggtcg atccttcctt
     5281 gttcaagccg aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg
     5341 cattatggat ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt
     5401 cgttgtcgca cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggcaataa
     5461 agtcgaactg ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca
     5521 gagcttcacc attgaagggc tggcggttgg agttattcgc aacggcgact ggctgtaaca
     5581 tatctctgag accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag
     5641 gcttgtctgc atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc
     5701 catgattttc tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg
     5761 tcatcttgat agtccagttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt
     5821 tctctttatg ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta
     5881 tggtgccaaa aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt
     5941 gggggcaggg gcgttaattg cgctgctgcg tacaccgatt atcgatctgg cactgcatat
     6001 tgttggcggt agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt
     6061 aagcgcaccg gcgtcgctgg cgaatctggt attacttggt tggttactcg gcgtgcaata
     6121 tgcccgtgcg ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt
     6181 ctggctggtg atggggctgc atatgaacgt gcagggcgcg gcgctggcga cggttattgc
     6241 ggaatatgca acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg
     6301 aatttccggc gaaatgctga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct
     6361 taaccgcgat atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt
     6421 acttggtgcg cgactgggga gtgacattat cgctgttaac gcggttctga tgacgctact
     6481 cacctttacc gcctatgcgc tggatggttt tgcctacgcg gttgaagcgc attccggtca
     6541 ggcgtacggt gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca
     6601 gtcggggatc gtagcgttac tgttttcggt agtttatttg ctggcagggg aacacatcat
     6661 tgcgttgctg acgtcgttaa cccagattca gcagctggcc gaccgctatc ttatctggca
     6721 ggtgattttg ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc
     6781 aacgcgcgca gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac
     6841 gctccttacg ctgccgtggc tgggtaatca tgctttgtgg ctggcgttaa ccgtctttct
     6901 ggcgttgcgc gggctttctc tggcggctat ctggcggcgt cactggcgca acggtacctg
     6961 gtttgccgca atgtgacggt taaaaattct gaataaataa tcctaagcca aattgctgac
     7021 tacacttaat ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg
     7081 aggtcttgat tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag
     7141 tgaaagagca atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg
     7201 atcaactggt cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag
     7261 aggtcgtgga ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac
     7321 gtgtacaagg atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat
     7381 gctggcactg ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat
     7441 gtgcttcaat cacattatgc cgcagggcaa aacccatttt tgccgccagc gtatgcatga
     7501 tgtcttccac gccttcagca cactcttctt tcaccgcgcc gcagcgatcg caaataaaca
     7561 tggctgacgt atgggtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt
     7621 ccaccttatg cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg
     7681 gcttggcttg cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc
     7741 catcctgcag actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac
     7801 gctgcgcgca gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg
     7861 ggcacctcaa agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg
     7921 agaaccctta ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttgtgttgc
     7981 cttgtcgtac cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc
     8041 aagtta
//
LOCUS       DAJSKJ010000171         7875 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000171 DAJSKJ010000000
VERSION     DAJSKJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.173"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..3179
                     /gene="yfaL"
                     /locus_tag="ON345_003286"
     CDS             <1..3179
                     /gene="yfaL"
                     /locus_tag="ON345_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR3073302.1"
                     /translation="DVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTA
                     FTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAG
                     GFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDSHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPDP
                     DPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGNGQTLNLR
                     VIGGRYHYTAAGQLAQQEDTSTVQLSGDLFSGRWGDDGEWMLGAVGGYSDNQGESRSN
                     MTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEQDDGTDHYHS
                     SGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLG
                     LHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTGNISQRV
                     SLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3307..4029)
                     /locus_tag="ON345_003287"
     CDS             complement(3307..4029)
                     /locus_tag="ON345_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR3073303.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4176..6803
                     /gene="gyrA"
                     /locus_tag="ON345_003288"
     CDS             4176..6803
                     /gene="gyrA"
                     /locus_tag="ON345_003288"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR3073304.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6952..>7875
                     /locus_tag="ON345_003289"
     CDS             6952..>7875
                     /locus_tag="ON345_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCR3073305.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFD"
BASE COUNT         1924 a         1916 c         2229 g         1806 t
ORIGIN      
        1 atgatgtttt taacaataac caggcatata catcaacaag ttacagtgat ggcgatggcg
       61 gggcaatcga tgttaccgat aataatagcg acagcaagca tccttcaggt tatacgataa
      121 taaataacac cgcctttaca aataacacag ccgaaggtta tggcggggcg atatatacca
      181 atagcgcgac ggctccctat cttattgata tttctgttga tgacagctac agccagaacg
      241 gcggcgtgtt agtcgatgag aacaatagcg cagcaggcta tggagatggt ccttccactg
      301 cggcgggtgg ctttatgtat ctcggcttaa gtgaagttac ctttgatatt gctgacggaa
      361 aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt gctggtaccg
      421 ggttaatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac aatgacttta
      481 ctggtgagat gcagattgaa aacggtgaag ttaccctggg tcgcagcaac tccctgatga
      541 atgtcggaga tagtcattgc caggacgatc cgcaagactg ctacggtctg acgataggga
      601 gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa caaacctttg
      661 tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt ggcaatgtta
      721 ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc accattgcgc
      781 aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc gatatagtcg
      841 ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc gctctccagg
      901 acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat ttctccacct
      961 ggcagagcgg tacatcatac aacgatggcc ttgaagtcag tggcagcagc ggaacggtta
     1021 tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc ggcggcgacg
     1081 ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt ctggcaaaca
     1141 ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg agcgacaact
     1201 cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag caacaagaaa
     1261 gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag catgggcgtg
     1321 atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac acgcagtggg
     1381 gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca tttactaaaa
     1441 cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca gtacgtgtcg
     1501 aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg tcactgtggg
     1561 tcggtgatgg ggcgacgttc gttactggcg cggatcagga tattcagtct atcgatgcta
     1621 cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc gggcaggata
     1681 cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg aatgctaccg
     1741 atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc ctgacctatc
     1801 ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt agcctgcaaa
     1861 atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc ggtacgctac
     1921 tgttcgacag cgaattaaac ggcgatgact cggtaagcga tcaattggtg atgaacggta
     1981 atactgctgg caacacaact gtggtggtta actccattac agggattggt gagccgacat
     2041 cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa aacaatgcac
     2101 agttcagtct ggctggcagt ggctacgtca acatgggagc gtatgactac acgctagtgg
     2161 aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg ccacctgatc
     2221 cagacccgga cccaacaccc gatcctgacc caacacccga tccaatcccc gcttaccagc
     2281 cggtgctgaa tgccaaagtt ggcggttatc tcaataatct gcgggcggca aatcaggcgt
     2341 ttatgatgga gcgacgcgat catgcaggtg gcaatggtca gacgctgaat ttacgtgtta
     2401 tcggcggacg ttatcattac acggcggcag ggcaactggc tcagcaggaa gacacttcta
     2461 cggtgcagct tagcggtgac ctgtttagcg ggcgctgggg cgatgatggc gagtggatgc
     2521 ttggggcggt tggtggctac agtgataatc agggcgagag ccgctcgaat atgaccggaa
     2581 ctcgcgccga taaccagaac cacggttatg ccgttgggct gacctcaagc tggtatcagc
     2641 acggtaatca gaagcagggg gcctggctgg atagctggct gcaatacgcg tggtttaaca
     2701 atgatgtttc cgaacaagac gatggcacag atcattacca ctcgtcgggg attatcgcct
     2761 cgctggaagc cgggtatcag tggttaccgg ggcgtggtgt ggtgattgaa ccgcaggcgc
     2821 aggtgattta tcagggcgtg cagcaggatg attttaccgc cgctaaccat gcgcgcgtgt
     2881 cacaatcgca gggggatgat attcagacgc ggctgggttt acacagcgaa tggcgtaccg
     2941 cggttggtgt cacaccaaca ttagatctga attattatca cgatccccat gcgacggaaa
     3001 ttgaagaaga tggcagcact atcagtgacg atgcggtgaa gcgacggggt gaaataaaag
     3061 tgggagtcac gggcaatatc agtcagcgag tgtccctgcg cggcagcgtg gcgtggcaga
     3121 aagggagtga tgattttgcc cagacggcag ggtttttgtc gatgacggtg aaatggtaat
     3181 cactgtctag tcggcacggt tgccggatgc ggcgtaaacg ccttatccgg cctacgggga
     3241 gcatttgtag gcctgataag acgcggcaag cgtcgcatca ggcattggct cggccaaaca
     3301 acaacctcac ttattctgcg tgtgcagcat atagttcaca tccacgccgg ggccgagttt
     3361 aaaagtatta gtgagcgggt tgtaatgtag cccggttata tgtcgctctt taaggcttgt
     3421 ctgatctacc cagccaagca gttctgccgg tttaataaac ttcttcacat catgcgtgcc
     3481 tttgggcacc atgcgcaaaa tatattccgc gccaaccacc gccatcagcc atgacttgcc
     3541 gttgcggtta agcgtcgaga aaaagacatc gccgcctggt ttcaccagtt gcgcacaggc
     3601 tctgaccact gactgcggat cggggacgtg ctccagcatc tccatgcagg tcaccacatc
     3661 atactgcccg gcatgttttg ccgcgtgctc ttccacggtt tcctgcacat aatccacctg
     3721 aatgccgctt tccagcgcgt gcagttttgc cacctgcaat ggctcaaagc ccatatccag
     3781 accggtcacc gtcgcgcctt cgcgcgccat actctcggcc agaatgccgc cgccacaacc
     3841 gacatcgagc acctttttgc caaataaacc gccagcacgc tcggcaatat agcccagacg
     3901 cagcgggtta atgcggtgca acggtttgaa ctcaccttca agatcccacc agcgggaggc
     3961 gacggcttca aatttagcga tctcttcgtg gtctacgtta tggttttccg gcgatttttc
     4021 ggcattcatt ggcacttcta ctccgtaatt ggcaagacaa acgagtatat caggcattgg
     4081 atgtgaataa agcgtatagg tttacctcaa actgcgcggc tgtgttataa tttgcgacct
     4141 ttgaatccgg gatacagtag agggatagcg gttagatgag cgaccttgcg agagaaatta
     4201 caccggtcaa cattgaggaa gagctgaaga gctcctatct ggattatgcg atgtcggtca
     4261 ttgttggccg tgcgctgcca gatgtccgag atggcctgaa gccggtacac cgtcgcgtac
     4321 tttacgccat gaacgtacta ggcaatgact ggaacaaagc ctataaaaaa tctgcccgtg
     4381 tcgttggtga cgtaatcggt aaataccatc cccatggtga ctcggcggtt tatgacacga
     4441 tcgtccgtat ggcgcagcca ttctcgctgc gttacatgct ggtagacggt cagggtaact
     4501 tcggttccat cgacggcgac tctgcggcgg caatgcgtta tacggaaatc cgtctggcga
     4561 aaattgccca tgaactgatg gccgatctcg aaaaagagac ggtcgatttc gttgataact
     4621 atgacggcac ggaaaaaatt cccgacgtca tgccaaccaa aattcctaac ctgctggtga
     4681 acggttcttc cggtatcgcc gtaggtatgg caaccaacat cccgccgcac aacctgacgg
     4741 aagtcatcaa cggttgtctg gcgtatatcg atgatgaaga catcagcatt gaagggctga
     4801 tggaacacat cccggggccg gacttcccga cggcggcaat cattaacggt cgtcgcggta
     4861 ttgaagaagc ttaccgtacc ggtcgcggca aggtgtatat ccgcgctcgc gcagaagtgg
     4921 aagttgacgc caaaaccggt cgtgaaacca ttatcgtcca cgaaattccg tatcaggtaa
     4981 acaaagcgcg cctgatcgag aagattgcgg aactggtaaa agaaaaacgc gtggaaggca
     5041 tcagcgcgct gcgtgacgag tctgacaaag acggtatgcg catcgtgatt gaagtgaaac
     5101 gcgatgcggt cggtgaagtt gtgctcaaca acctctactc ccagacccag ttgcaggttt
     5161 ctttcggtat caacatggtg gcattgcacc atggtcagcc gaagatcatg aacctgaaag
     5221 acatcatcgc ggcgtttgtt cgtcaccgcc gtgaagtggt gacccgtcgt actattttcg
     5281 aactgcgtaa agctcgcgat cgtgctcata tccttgaagc attagccgtg gcgctggcga
     5341 acatcgaccc gatcatcgaa ctgatccgtc atgcgccgac gcctgcagaa gcgaaaactg
     5401 cgctggttgc taatccgtgg cagctgggca acgttgccgc gatgctcgaa cgtgctggcg
     5461 acgatgctgc gcgtccggaa tggctggagc cagagttcgg cgtgcgtgat ggtctgtact
     5521 acctgaccga acagcaagct caggcgattc tggatctgcg tttgcagaaa ctgaccggcc
     5581 ttgagcacga aaaactgctc gacgaataca aagagctgct ggatcagatc gcggaactgt
     5641 tgcgtattct tggtagcgcc gatcgtttga tggaagtgat ccgtgaagag ctggagctgg
     5701 ttcgtgaaca gttcggtgac aaacgtcgta ctgaaatcac cgccaacagc gcagacatca
     5761 acctggaaga tctgatcact caggaagatg tggtagtgac gctctctcac cagggctacg
     5821 ttaagtatca gccgctttct gaatacgaag cgcagcgtcg tggcgggaaa ggtaaatctg
     5881 ccgcacgtat taaagaagaa gactttatcg accgactgct ggtggcgaac actcacgacc
     5941 atattctgtg cttctccagc cgtggtcgcg tctattcgat gaaagtttat cagttgccgg
     6001 aagccactcg tggcgcgcgc ggtcgtccga tcgtcaacct gctgccgctg gagcaggacg
     6061 aacgtatcac tgcgatcctg ccagtgaccg agtttgaaga aggcgtgaaa gtcttcatgg
     6121 cgaccgctaa cggtaccgtg aagaaaactg tcctcaccga gttcaaccgt ctgcgtaccg
     6181 ccggtaaagt ggcgatcaaa ctggttgacg gcgatgagct gatcggcgtt gacctgacca
     6241 gcggcgaaga cgaagtaatg ctgttctccg ctgaaggtaa agtggtgcgc tttaaagagt
     6301 cttctgtccg tgcgatgggc tgcaacacca ccggtgttcg cggtattcgc ttaggtgaag
     6361 gcgataaagt cgtctctctg atcgtgcctc gtggcgatgg cgcaatcctc accgcaacgc
     6421 aaaacggtta cggtaaacgt accgcagtgg cggaataccc aaccaagtcg cgtgcgacga
     6481 aaggggttat ctccattaag gttactgaac gtaatggttt agtggttggc gcggtacagg
     6541 tagatgactg cgaccagatc atgatgatca ccgatgccgg tacgctggta cgtactcgcg
     6601 tttcggaaat cagcatcgta ggccgtaaca cccagggcgt gatcctcatc cgtactgcgg
     6661 aagatgaaaa cgtagtgggt ctgcaacgtg ttgctgaacc ggttgacgag gaagatctgg
     6721 ataccatcga tggcagtgcc gcggaagggg acgatgaaat cgctccggaa gtggacgttg
     6781 acgacgagcc agaagaagaa taattttact tcttcatgcc aaaagggagc tatctccctt
     6841 gtttgaattg aaaagtccag gctgcaaagt ctgggctttt gtcgtattag ggcacggtaa
     6901 agtttggctg tgcccgtaaa aaatggctgg ctatacacaa ggaatgtggc aatgagtggt
     6961 gaaaaaaagg cgaaaggctg gcggttctat ggtcttgtag gttttggcgc aatagcactg
     7021 ctttccgctg gcgtctgggc gttgcaatat gctggcagtg ggccagaaaa aacgttgtcg
     7081 ccgctggtgg tgcacaacaa tctgcaaatc gatctcaatg agccggacct ctttctcgac
     7141 agcgactctc tgagccagct tcccaaagat ctcctcacca ttccgtttct ccacgatgtt
     7201 ctgagcgaag atttcgtttt ctattatcag aatcatgccg atcgtctggg cattgaaggc
     7261 agcattcgtc gtattgtcta tgaacacgat ctcacgctga aagataagct cttttcgtca
     7321 ctcttagatc agcccgcgca ggcagcgctg tggcacgata aacaaggcca tctttcacat
     7381 tatatggtgc tgatccagcg cagtggttta agcaaactgc tggagccatt gttgtttgcc
     7441 gctaccagcg acagccagtt aagcaaaacg gaaatcagta gcatcaagat aaatagtgaa
     7501 actgttcctg tttatcagtt gcgctataac ggcaataacg ctctgatgtt cgcgacatat
     7561 caggacaaga tgctagtgtt ttccagcacg gatatgctgt ttaaagatga tcagcaggat
     7621 accgaagcca cggcgatcgc aggtgatttg ttgagcggca aaaaacgctg gcaagcaagc
     7681 tttggcctgg aagagcgtgc tgctgaaaaa acgccagtac gccagcgcat cgtggtcagc
     7741 gccaggttgc tgggttttgg ctaccagcgg ttaatgcctt cttttgctgg cgtacgcttc
     7801 gaaatgggta acgacggctg gcacagtttt gtggcgttaa atgatgaatc cgccagcgta
     7861 gataccagtt tcgat
//
LOCUS       DAJSKJ010000172         7752 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000172 DAJSKJ010000000
VERSION     DAJSKJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.174"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(54..130)
                     /locus_tag="ON345_003290"
     tRNA            complement(54..130)
                     /locus_tag="ON345_003290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Arg,seq:acg)
     gene            complement(328..404)
                     /locus_tag="ON345_003291"
     tRNA            complement(328..404)
                     /locus_tag="ON345_003291"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Arg,seq:acg)
     gene            complement(408..500)
                     /locus_tag="ON345_003292"
     tRNA            complement(408..500)
                     /locus_tag="ON345_003292"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(464..466),aa:Ser,seq:gct)
     gene            complement(816..1001)
                     /gene="csrA"
                     /locus_tag="ON345_003293"
     CDS             complement(816..1001)
                     /gene="csrA"
                     /locus_tag="ON345_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR3073306.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1236..3866)
                     /gene="alaS"
                     /locus_tag="ON345_003294"
     CDS             complement(1236..3866)
                     /gene="alaS"
                     /locus_tag="ON345_003294"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR3073307.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3994..4494)
                     /gene="recX"
                     /locus_tag="ON345_003295"
     CDS             complement(3994..4494)
                     /gene="recX"
                     /locus_tag="ON345_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR3073308.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4737..5798)
                     /gene="recA"
                     /locus_tag="ON345_003296"
     CDS             complement(4737..5798)
                     /gene="recA"
                     /locus_tag="ON345_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR3073309.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5878..6375)
                     /gene="pncC"
                     /locus_tag="ON345_003297"
     CDS             complement(5878..6375)
                     /gene="pncC"
                     /locus_tag="ON345_003297"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR3073310.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6520..7605)
                     /gene="mltB"
                     /locus_tag="ON345_003298"
     CDS             complement(6520..7605)
                     /gene="mltB"
                     /locus_tag="ON345_003298"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR3073311.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1803 a         2178 c         1866 g         1905 t
ORIGIN      
        1 ggttaattcc gctacctctt cgataccttc attgctgaag atttcggaga atatggtgca
       61 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
      121 ctacggatgc atcgggaaac ttactttact gcagattttt tgataccgct actaaagccg
      181 tatcaagtaa gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc
      241 cgttgctaaa gcaacgttat cctccctggt gcctgcggtt ataccgccac ctcttccata
      301 ccttcatcgc tgaaagtacg tagaatatgg tgcatccggg aggattcgaa cctccgaccg
      361 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
      421 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
      481 agcctctcgg ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc
      541 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
      601 ctacactttt atcgtttgca cacttcacgt tcaattagtc tgtaaaaacg tcaaaaagag
      661 tgttttatca acagaaaaat ggaggtctga cagatagtag taatgcaaaa aaatggagac
      721 ttaagttgaa tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg
      781 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
      841 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
      901 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
      961 atcatgaggg tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
     1021 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
     1081 caatgacaaa cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt
     1141 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
     1201 gtgaagcgag tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag
     1261 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
     1321 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
     1381 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
     1441 tcggctaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
     1501 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
     1561 ttaacatcaa ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta
     1621 agctgttgta gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc
     1681 gccagattat tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct
     1741 gcatgaacgg tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca
     1801 gcagtacccg attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga
     1861 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca
     1921 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
     1981 ttggtttcga tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga
     2041 atctcttccg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta
     2101 accagtgaac ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc
     2161 aggtgcgttg cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc
     2221 tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
     2281 tggccgtatt tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct
     2341 ttatcaccaa cctgaccgcc ggattccgca tagaatggtg tttgatccag cacgaccaca
     2401 gcttcctggc ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact
     2461 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc
     2521 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc
     2581 attgctgctt caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
     2641 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc
     2701 gtatcaccag aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta
     2761 cgggcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
     2821 aggtcttcac ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag
     2881 gtttctttcg cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt
     2941 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
     3001 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact
     3061 ttcgctaccg cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
     3121 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc
     3181 ggttccatcg tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca
     3241 atgtagcggt cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg
     3301 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
     3361 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
     3421 atcccgactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
     3481 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
     3541 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
     3601 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
     3661 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
     3721 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
     3781 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
     3841 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
     3901 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
     3961 tttcttgact gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc
     4021 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
     4081 tgaaaagaca gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcgccagtgc
     4141 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
     4201 ctgattcagt tcctgacgaa tacgtgcagg tccataacct ttgcggctac ggctggcgat
     4261 aaagcgcgca acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
     4321 gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg
     4381 tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
     4441 cgcacgatcc aacaggcgag catatgccgg gcgacgggac gttgattctg tcatggcata
     4501 tccttacaac ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca
     4561 gatttaatag tcaatgagtg tgcagaatgt tcccgcaggc ccaattcgtt agaggatcga
     4621 ggtgagagta atttttcacg ccagtgctgg cctgttcccc tcaccctaac cctctcccca
     4681 aaggggcgag gggaccgtcc gtgcacacat tatctattat catcaataac gaacgattaa
     4741 aaatcttcgt tagtttctgc tacgccttcg ctgtcatcta cagagaaatc cggcgttgag
     4801 ttcgggttgc tcagcagcaa ctcacgtact ttcttctcga tctctttcgc ggtttccggg
     4861 ttatctttca gccaggcagt tgcattcgct ttaccctgac cgatcttctc acctttgtag
     4921 ctgtaccacg cgcctgcttt ctcgatcagc ttctctttta cgcccaggtc aaccagttcg
     4981 ccgtagaagt tgataccttc gccgtagagg atctgaaatt cagcctgttt aaacggtgca
     5041 gcgattttgt tcttcaccac tttcacgcgg gtttcgctac ccaccacgtt ttcgccctct
     5101 ttcaccgcgc cgatacgacg gatgtcgaga cgaacagagg cgtagaattt cagcgcgtta
     5161 ccaccggtag tggtttccgg gttaccgaac atcacaccaa ttttcatacg gatctggttg
     5221 atgaagatca gcagcgtgtt ggactgcttc aggttacccg ccagcttacg catcgcctgg
     5281 ctcatcatac gtgccgcaag gcccatgtga gagtcgccga tttcgccttc gatttccgct
     5341 ttcggcgtca gtgccgccac ggagtcaacg acgataacgt ctactgcgcc agaacgcgcc
     5401 agggcgtcac agatttccag tgcctgctcg ccggtgtccg gctgggagca cagcaggtta
     5461 tcgatatcga cgcccagttt acgtgcgtag attgggtcca gcgcgtgttc agcatcgata
     5521 aacgcacagg ttttaccttc acgctgcgct gcggcgatca cctgcagcgt cagcgtggtt
     5581 ttaccggaag attccggtcc gtagatttcg acgatacggc ccatcggcag accacctgcc
     5641 ccaagcgcga tatccagtga aagcgaaccg gtagagatgg tttccacatc catggaacgg
     5701 tcttcaccca ggcgcatgat ggagccttta ccaaattgtt tctcaatctg gcccagtgct
     5761 gccgccaacg ctttctgttt gttttcgtcg atagccattt ttactcctgt catgccgggt
     5821 aataccggat agtcaatatg ttctgttgaa gcaattatac tgtatgctca tacagtatca
     5881 agtgttttgt agaaattgtt gccacaaggt ctgcaatgca tacgcagtag cctgacgacg
     5941 caccgcatca cggtcgccgc tgaagcattc ccgccgggta atgccttcac cgcgggcagt
     6001 ggcaaaagca aaccagacgg tgccgacagg cttctcttca ctgccgccat ccggcccggc
     6061 gataccacta atagacacgg cataatcagc acgagccgct ttcagtgcgc ctatcaccat
     6121 ttccaccacg acgggttcac tcaccgcgcc atgctgcgcc agcgtctctt cgcgtacgcc
     6181 gatcatctgc gctttggctt cgttactgta ggtgacaaat ccgcgttcaa accaggcgga
     6241 gctaccggca atatcggtaa tcactttcgc tacccaacca ccggtacaag actcggcagt
     6301 tgttacggtt gcgccacggg ctttcagcgc ctgcccaacc tgttcactta actgcatcag
     6361 ttcactgtca gtcatcacaa cctctgtcag tcaaaaattt atgccggaca agatagcact
     6421 ttcgtcagga agatggtgtt ggcgttgcaa ttttacgagg aggattcaga aaaaagctga
     6481 ttagccagag ggaagctcac gcccccctct tgcaaatagt tactgcacgc gcgccagcgc
     6541 cacggcttgt cctaactgcc agaccgccat tgcgtaatgg gtgctgtggt tataacgggt
     6601 aatggtgtag aagttcggca gaccgtacca gtactggtaa ccggtaccga catccagacg
     6661 cagcaggctg gcttgttgat ggttgcccag cggctgctgt ggcgttaaac ctgccgtggc
     6721 aagctgcgaa atgctgtacc tggttttgaa gccatttggc aagcctggag cctgaccgtt
     6781 tgccattacc gcgacctgat cgcctttcac ccagccgtgc gctttgaaat agttcgccac
     6841 gctaccgatc gcatcaaccg gatcccacag gttgatatgc ccgtcgccgc tgaaatctac
     6901 cgcatattgt ttgtaagacg acggcataaa ctgtccgtag cccatcgccc cggcaaagga
     6961 acctttcaga ttgagcggat cgtcctgctc gtcgcgcgcc atcaacagga aggtttccag
     7021 ctcgccagag aaatactccg cgcggcgtgg gtagttaaat gacagcgttg ccagcgcatc
     7081 gaggatgcga gttttcccca tcacgcgccc ccagcgggtt tcaacgccga taatcccgac
     7141 gataatttcc ggcggtacgc catacacctg ccacgcgcga ttcaacgcat cttcatactg
     7201 attccagaaa accacaccgt tctgcacgtt gtccggcgta ataaattttt tgcgataacg
     7261 gagccatgcg ccgttcggac ctgatggcgg tttcaccgat gtggttggtg cctggttatc
     7321 catcagccgc agtaccgaat ccagacgctt cgcctgggag agaatttcct gcaactgctg
     7381 acgatcgaaa ccgtgtttgt tcaccatttt gtcgatgaac tgctgggcat tcgggttatt
     7441 agcgaaatcg ccgcccatct gcatcacatt gtgctgcggc tcaagcagga agccgccaga
     7501 cggcgttccg gtggtcgttt cagtctcagt aggttttggc ttgctgctac aggcggcaag
     7561 caacacaaaa aggggaagca atgttacata acgacgcttg aacatgaggg gtccatttaa
     7621 cagattcaac caggggcaag tatggtaaag catcacgccc cacacaagga agcggtagtc
     7681 actgcccgat acggacttta cataactcaa ctcattcccc tcgctatcct tttactcaaa
     7741 ctttcaaatt aa
//
LOCUS       DAJSKJ010000173         7567 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000173 DAJSKJ010000000
VERSION     DAJSKJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.175"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            114..1511
                     /gene="ulaA"
                     /locus_tag="ON345_003299"
     CDS             114..1511
                     /gene="ulaA"
                     /locus_tag="ON345_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3073312.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTVILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQFA"
     gene            1527..1832
                     /gene="ulaB"
                     /locus_tag="ON345_003300"
     CDS             1527..1832
                     /gene="ulaB"
                     /locus_tag="ON345_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR3073313.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1842..2306
                     /gene="ulaC"
                     /locus_tag="ON345_003301"
     CDS             1842..2306
                     /gene="ulaC"
                     /locus_tag="ON345_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR3073314.1"
                     /translation="MKLRDSLAVNKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2320..2970
                     /gene="ulaD"
                     /locus_tag="ON345_003302"
     CDS             2320..2970
                     /gene="ulaD"
                     /locus_tag="ON345_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR3073315.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2980..3834
                     /gene="ulaE"
                     /locus_tag="ON345_003303"
                     /pseudo
     CDS             2980..3834
                     /gene="ulaE"
                     /locus_tag="ON345_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            3834..4520
                     /gene="ulaF"
                     /locus_tag="ON345_003304"
     CDS             3834..4520
                     /gene="ulaF"
                     /locus_tag="ON345_003304"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR3073316.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4650..4925)
                     /locus_tag="ON345_003305"
     CDS             complement(4650..4925)
                     /locus_tag="ON345_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR3073317.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQDRNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5252..5647
                     /gene="rpsF"
                     /locus_tag="ON345_003306"
     CDS             5252..5647
                     /gene="rpsF"
                     /locus_tag="ON345_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR3073318.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5654..5968
                     /gene="priB"
                     /locus_tag="ON345_003307"
     CDS             5654..5968
                     /gene="priB"
                     /locus_tag="ON345_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR3073319.1"
                     /translation="MTNRLVLSGTVCRAPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5973..6200
                     /gene="rpsR"
                     /locus_tag="ON345_003308"
     CDS             5973..6200
                     /gene="rpsR"
                     /locus_tag="ON345_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR3073320.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6242..6691
                     /gene="rplI"
                     /locus_tag="ON345_003309"
     CDS             6242..6691
                     /gene="rplI"
                     /locus_tag="ON345_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR3073321.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6762..7556)
                     /locus_tag="ON345_003310"
     CDS             complement(6762..7556)
                     /locus_tag="ON345_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR3073322.1"
                     /translation="MTLPITIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLAMACNAYDGITMKGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGSERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1797 a         1845 c         2073 g         1852 t
ORIGIN      
        1 gcgtcacatt taatcataaa taatcttgtt gtgattactt ttgaaaatta gagtgagtgc
       61 acaacattcc gggtgtgtgg aatacccggt tacctcttct tcaggagagc gttatggaga
      121 tcctctacaa catctttacc gtgtttttta accaggtcat gaccaatgcc ccgttgttgc
      181 tgggtattgt gacctgtctg ggctacatcc tactgcgcaa aagtgtcagc gttattatta
      241 aaggcacgat taaaaccata attggtttca tgttgttgca ggcagggtcc ggcatcctca
      301 ccagcacctt caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa
      361 tttccgatac ctacgcttca atgatggcga ccatcgaccg catgggcgat gcctatagct
      421 gggtgggtta tgccgtattg ttagcgctgg cgctgaacat ctgttacgtg ctgttgcgtc
      481 gcattaccgg cattcgcaca atcatgttga ccggccacat catgttccag caggccgggt
      541 tgattgccgt tacgctgttt atcttcggct actccatgtg gaccaccatt atctgtaccg
      601 tgattctggt ttcgctctac tggggcatca cttccaacat gatgtacaag ccgactcagg
      661 aagtgacgga tggctgtggt ttctccatcg gtcaccagca gcagtttgca tcatggattg
      721 cctataaagt cgcgccgttc ctcggcaaaa aagaggagag cgttgaagac ctcaaattgc
      781 cgggctggct gaacattttc cacgacaaca tcgtctccac ggcgattgtg atgaccatct
      841 tctttggcgc cattctgctc tccttcggta tcgacaccgt gcaggcgatg gcgggcaaag
      901 tgcactggac ggtctacatc ctgcaaaccg gtttctcctt cgcggtggcg atcttcatca
      961 tcactcaggg cgtgcgcatg tttgtggcgg aactctctga agcatttaac ggtatctccc
     1021 agcgcctgat cccaggtgcg gttctggcga ttgactgtgc ggctatctat agcttcgcgc
     1081 cgaacgccgt ggtctggggc tttatgtggg gcaccatcgg tcagctgatt gcagttggca
     1141 tcctggttgc ctgcggctct tccattctga ttattccagg ctttatcccg atgttcttct
     1201 ccaacgccac catcggcgtg ttcgctaacc acttcggcgg ctggcgtgca gcgctgaaga
     1261 tttgtctggt gatgggaatg atcgaaatct ttggttgcgt ctgggcggtg aaactcaccg
     1321 gtatgagtgc ctggatgggc atggcggact ggtcgattct ggcaccgccg atgatgcaag
     1381 gcttcttctc catcggtatc gcctttatgg ccgtcatcat tgtaattgca ctggcttata
     1441 tgttcttcgc tggccgcgcg ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac
     1501 agtttgctta ataaggagtt ttgattatga ccgtacgtat tctggctgtg tgtggcaacg
     1561 gacaaggcag ttccatgatc atgaagatga aagtggacca gtttttaacc caatcaaaca
     1621 ttgaccacac ggtaaacagc tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg
     1681 atatcatcat cgcttctacg cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg
     1741 tggttggcgt gcgcaacatg ctctctcctg ctgactttgg cccgaaactg ctggaagtga
     1801 tcaaagagca tttcccgcag gacgtgaagt aaggacgcgc catgaaatta cgtgattcgc
     1861 tggctgtaaa taaatccatc cgcctgcagg ctgaagctga aacctggcag gacgcggtga
     1921 aaatcggcgt cgatctgctg gtggcggcgg acgttgtcga gccgcgctat taccaggcga
     1981 ttctggatgg cgtggaacag tttggccctt atttcgttat cgctccgggg ctggcgatgc
     2041 cgcacgggcg gccggaagag ggtgttaaga aaaccggttt ctctctggta acgctgaaaa
     2101 agccgctgga atttaaccac gacgacaacg atccggtgga tatcctcatc accatggcgg
     2161 cggtcgatgc caatactcac caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag
     2221 atgaagagaa ttttgaccgt ttacgcgcct gccgtaccga gcaggaagta ctggatctca
     2281 ttgaccgcac caacgcggca gcttaagaag gaattgaaca tgtcattacc gatgttgcaa
     2341 gtcgcgctgg acaaccagac tatggatagc gcctacgaaa ccactcgcct gattgccgaa
     2401 gaagtcgaca ttatcgaagt gggcaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt
     2461 cgtgacctga aagcgctcta cccgcacaaa atcgtactgg cagacgccaa aattgccgat
     2521 gcaggcaaaa tcctttcgcg tatgtgcttc gaagccaacg ctgactgggt gacggtaatt
     2581 tgctgtgcgg atatcaacac cgccaaaggc gcgctggacg tggcaaaaga gtttaacggc
     2641 gacgtgcaga tcgaactgac cggttactgg acctgggaac aggcgcaaca gtggcgcgat
     2701 gcaggcattc agcaggtggt ttatcaccgc agccgtgacg cgcaggccgc aggcgtggcg
     2761 tggggcgaag cggacatcac cgcgatcaaa cgtctttccg atatgggctt caaagtcacc
     2821 gtcaccggag gcctggcgct ggaagatctg ccgctgttca agggtattcc gattcacgtc
     2881 tttatcgcgg gccgtagtat ccgtgatgcc gcttctccgg tggaagccgc acgtcagttc
     2941 aaacgttcca tcgctgaact gtggggctaa ggagcggata tgttgtccaa acaaatcccg
     3001 cttggcatct atgaaaaagc gctccccgcc ggggagtgct ggctggaacg cctgcaactg
     3061 gcaaaaacgt tgggcttcga ttttgtcgag atgtcggtag atgaaacgga cgagcgcctg
     3121 tcgcgcctcg actggagccg cgagcagcgt ctggcgctgg tcaatgcgat tgttgaaacc
     3181 ggcgtgcgcg tgccgtccat gtgcctttct gctcatcgtc gtttcccgct gggcagtgaa
     3241 gatgacgcgg tgcgggcgca ggggctggag attatgcgta aagctatcca gttcgcccag
     3301 gatgtcggta ttcgcgtgat ccagctggcg ggctatgacg tttactatca ggaagccaat
     3361 aacgaaacgc gtcgtcgttt ccgtgacggc ctgaaagaga gcgttgagat ggcaagccgc
     3421 gcgcaggtga cgctggcgat ggagatcatg gattatccgt tgatgaactc catcagcaag
     3481 gcgctgggct acgcgcacta tctcaacaat ccgtggttcc agctctaccc ggatatcggc
     3541 aacctgtcgg cgtgggataa cgacgtgcag atggagttgc aggccggaat cgggcatatc
     3601 gtcgcggtgc atgtgaaaga caccaaaccg ggcgtcttca aaaacgtgcc gtttggcgaa
     3661 ggtgtagtgg atttcgaacg ttgtttcgaa acgctcaaat agagtggcta ttgcgggccg
     3721 tacctgattg agatgtggag cgaaacggcg gaagacccgg cggcagaagt ggcgaaagcg
     3781 cgtgattggg tgaaagcgcg catggcgaaa gcgggcatgg tggaggcggc ataatgcaaa
     3841 agctaaaaca gcaggtgttt gaagccaaca tggatctgcc gcgctatggg ctggtgacct
     3901 ttacctgggg caacgtcagc gctatcgacc gcgaacgcgg gctggtggtg atcaagccca
     3961 gcggcgttgc ctacgaaacc atgaaagcgg ccgatatggt ggtggttgat atgagcggca
     4021 aggtggtgga aggggagtat cgcccatctt ccgacactgc gacgcatctc gaactctacc
     4081 gtcgttaccc gtcgcttggc ggcattgtcc atacccactc cactcatgcc accgcatggg
     4141 cgcaggcggg gctggcgatc ccggcgttag gcaccacgca cgctgactac ttctttggcg
     4201 acattccgtg tacgcgcggg ttaagcgaag aagaggtgca gggcgagtat gaactgaaca
     4261 ccggcaaagt gattatcgaa acgctgggca acgccgagcc gctgcatacg ccgggaattg
     4321 tggtgtatca gcacggaccg ttcgcctggg ggaaagatgc ccacgatgcg gtgcataacg
     4381 cggtggtgat ggaagaagtg gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac
     4441 tcaatcacat cgacagcttc ctgatgaata aacacttcat gcgtaagcac ggtcccaacg
     4501 cctattacgg gcagaagtaa aacacgcgct gcggaaattt ccttattcgg gagataactg
     4561 gtctgattcc gcagccgttt ttcaaaaaaa tgcccccgga gaagggggca aagcaaacta
     4621 tggcaatgtt tcgttggtta tacctggtgc tagcgataaa tatccgcgct ggcgtgcata
     4681 ttgccgttac tccccggttc tcgcatcaga attacgtggt agtacgttgc gccttgcgca
     4741 tctgtttctt catttaatgc ctgacgtgct tcactttcag tggcgaagtt atgattgata
     4801 taaatcacgc ctaagctttg cacatcgtcc atatttctgt cctggtggtt attaatttca
     4861 atggctgccc atgtatttgc acttagcaaa agcacagcca gaagggctaa aacacgactg
     4921 aacatagata cctcctcgac ggctgacttt gtgtgctctc ctctgtgatg atcttctgat
     4981 ttaattttaa tcaatgataa agaagttgat ggtgaccatt tctgatgcag ttgttcaaaa
     5041 aaacaccatg atgaagtgtg atgaacttca aatcagcgtg ttagtccact ttggtgaaag
     5101 atgagattta tttgtcggct ggccttgagt ttagcggggt atgtcagtac tataacgcgt
     5161 catttttcag ccgaccttta acacgttcct tgcctccccg ggattcggct gacccagaca
     5221 ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat tacgaaatcg tttttatggt
     5281 ccatcctgac cagagcgaac aggttccggg catgatcgag cgctacactg ctgccatcac
     5341 tggtgcagaa ggcaagatcc accgtctgga agactggggc cgccgtcagc tggcttaccc
     5401 gatcaacaaa ctgcacaaag cacactacgt tctgatgaac gttgaagctc cgcaggaagt
     5461 gatcgatgag ctggaaacta ccttccgctt caacgatgcc gttatccgca gcatggttat
     5521 gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt aaagcgaaag acgagcgccg
     5581 tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat gctgaagctg gggattctga
     5641 agagtaattt ctgatgacca accgtctggt gttgtccggc accgtgtgca gggctcccct
     5701 tcgaaaggtc agtccatcag gaattcctca ctgccagttc gtgcttgagc atcgttctgt
     5761 gcaggaggaa gccggctttc accggcaggc gtggtgtcaa atgcccgtta ttgttagcgg
     5821 acacgaaaac caggccatta ctcacagtat aacggtcggc agtcgcataa ccgttcaggg
     5881 gttcatttca tgccacaagg caaagaacgg actgagcaaa atggttttgc atgccgagca
     5941 gattgaattg atagattctg gagactagcc atatggcacg ttatttccgt cgtcgcaagt
     6001 tctgccgttt caccgcggaa ggcgttcaag agatcgacta taaagatatc gctacgctga
     6061 aaaactacat caccgaaagc ggtaagattg tcccaagccg tatcaccggt acccgtgcaa
     6121 aataccagcg tcagctggct cgcgctatca aacgcgctcg ctacctgtcc ctgctgccgt
     6181 acactgatcg ccatcagtaa tcggtcacgg tccattaata cgactttgag aggataaggt
     6241 aatgcaagtt attctgcttg ataaagtagc aaacctgggt agcctgggtg atcaggtaaa
     6301 cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag ggtaaagctg ttccagctac
     6361 caagaaaaac attgaattct tcgaagcacg tcgcgctgaa ctggaagcta aactggctga
     6421 agttctggca gctgctaatg ctcgcgctga gaaaatcaat gcactggaaa ctgttaccat
     6481 cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc atcggtactc gcgacatcgc
     6541 tgacgctgta actgcagctg gcgttgaagt ggctaagagc gaagttcgtc tgccgaacgg
     6601 cgttctgcgt accactggcg aacacgaagt gagcttccag gttcacagcg aagtattcgc
     6661 gaaagtgatc gtaaacgtag tagctgaata attcgttatt caacgagacg taaaaacgcc
     6721 gaccattggt cggcgttttg ctttctattt ttcgtcaggt attagtttcg caagtagatc
     6781 cattcaccat tctcttgtgt ccataagtca ggatggtttg ctggcgttag atacatacga
     6841 gcgcaactga tgtctcgctc ctgcggcact cgctcggaac ccaaacccga gtagattgcg
     6901 gctgaacttg ataaggaacg cagaccaatt ttcaaatcat ggggtaaatc agaatacgcg
     6961 tttagcaatg cagcaatgat gaatccgagg gcattagaaa cgcctgtaac agtgcgaagt
     7021 tggtcgatat aaagatcgat tgttggtagt tcagatgacc agcaattagg catttgggca
     7081 ggaagggtta caacgtatcc tatcgtttgg ccggtatagt ctggagtacc ataaccaggt
     7141 cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt cgaaggattc gacatcttgc
     7201 aataaatatt tcagttctgc ggttaaccgc gcacccgcgt aagctttatt gtatttctgg
     7261 gcgtgaatat tatctaatgt agcttcatgg ttccatccaa aagaattatc acccttcatt
     7321 gttattccat cgtaagcatt acacgccatc gctagtttat tgaatgcttg ccagtctgag
     7381 agatctagtt tgtggatctc aacagtacgt ctgccatcgc aattatgatc tcttccacga
     7441 tttgacagta atgtagcgcg tgcgtattga tgaaatttta gttttgctgg tttgtcggtg
     7501 gacgaaaaac gaggtaaata tgcggggaat gaataaatag ttattggtaa tgtcatttta
     7561 ttgttgc
//
LOCUS       DAJSKJ010000174         7390 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000174 DAJSKJ010000000
VERSION     DAJSKJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.176"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            181..549
                     /gene="sufA"
                     /locus_tag="ON345_003311"
     CDS             181..549
                     /gene="sufA"
                     /locus_tag="ON345_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR3073323.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            558..2045
                     /gene="sufB"
                     /locus_tag="ON345_003312"
     CDS             558..2045
                     /gene="sufB"
                     /locus_tag="ON345_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR3073324.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            2055..2801
                     /gene="sufC"
                     /locus_tag="ON345_003313"
     CDS             2055..2801
                     /gene="sufC"
                     /locus_tag="ON345_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR3073325.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            2776..4047
                     /gene="sufD"
                     /locus_tag="ON345_003314"
     CDS             2776..4047
                     /gene="sufD"
                     /locus_tag="ON345_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR3073326.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAKLTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            4044..5264
                     /gene="sufS"
                     /locus_tag="ON345_003315"
     CDS             4044..5264
                     /gene="sufS"
                     /locus_tag="ON345_003315"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR3073327.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTDNPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            5277..5693
                     /gene="sufE"
                     /locus_tag="ON345_003316"
     CDS             5277..5693
                     /gene="sufE"
                     /locus_tag="ON345_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR3073328.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQSLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            5842..6846
                     /gene="ldtE"
                     /locus_tag="ON345_003317"
     CDS             5842..6846
                     /gene="ldtE"
                     /locus_tag="ON345_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR3073329.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(6910..7146)
                     /gene="lpp"
                     /locus_tag="ON345_003318"
     CDS             complement(6910..7146)
                     /gene="lpp"
                     /locus_tag="ON345_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR3073330.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         1867 a         1778 c         2016 g         1729 t
ORIGIN      
        1 ctaacaatga gatacctaat tcttagcgtg cctgttaacc cacacatcag ggtctatgct
       61 tattaaaagc aggcaaaaag catgttttac ggtaaagccc ctgcgtttgc tgggttgaac
      121 tgataatcat tatcactaac atgctgttat acgctgaaag cgatgaagtg aggtaaatcg
      181 atggacatgc attcaggaac ctttaaccca caagatttcg cctggcaagg cttaacgctg
      241 acacccgcag cggcgataca catccgtgag ctggtggcaa agcagccggg tatggtcggc
      301 gtgcgcttag gcgtgaagca aacgggctgc gcgggctttg gctatgtgct cgacagtgtt
      361 agcgagccgg acaaagacga tctgctgttt gaacacgacg gcgcgaagct gtttgtcccg
      421 ctgcaagcga tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga aggacttaat
      481 cagatattca aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg cgaaagcttt
      541 ggggtatagg cggtactatg tctcgtaata ctgaagcaac tgacgatgtc aaaacctgga
      601 ccggcggccc gctgaattat aaagaaggat tcttcaccca gttagccacc gatgagctgg
      661 caaaggggat aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat gagccggagt
      721 ggatgctgga gtttcgtcta aacgcctatc gcgcatggct ggagatggaa gaaccgcact
      781 ggttgaaagc gcactacgac aagctgaatt atcaggatta cagctactac tcagcaccat
      841 cgtgcggtaa ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag caaactggcg
      901 cgaacgcctt tttaagtaaa gaggtggagg cggcgtttga gcagttgggc gttcccgtgc
      961 gggaaggcaa agaggtggcg gtggatgcca ttttcgactc tgtttcggtt gccactactt
     1021 atcgcgaaaa actggcggag cagggaatta ttttctgttc ctttggtgag gcgatccacg
     1081 atcacccgga actggtgcgt aaatatctcg gcaccgtggt gccggggaat gacaacttct
     1141 ttgccgcgct taatgcggcg gtagcctctg acggtacgtt tatttatgtg cctaaaggcg
     1201 tgcgctgccc gatggaactt tccacctatt ttcgcattaa cgcggaaaaa accgggcagt
     1261 ttgagcgcac cattctggtg gccgatgaag acagctacgt cagctacatt gaaggctgtt
     1321 ccgctccggt gcgtgacagc tatcagttac acgcggcagt ggtggaagtc atcatccata
     1381 aaaacgccga ggtgaaatat tccacggtac aaaactggtt ccctggcgat aacaacaccg
     1441 gcggtattct caacttcgtc accaagcgtg ctttgtgcga aggcgaaaac agcaaaatgt
     1501 catggacgca atcagaaacc gggtccgcga ttacgtggaa atatcccagc tgcattttgc
     1561 gcggcgataa ctccattggt gagttttact cagtggcgct gaccagcggt catcagcaag
     1621 cggataccgg caccaagatg atccacatcg gtaaaaacac caaatcgacc attatctcga
     1681 aagggatctc tgccggacat agtcagaaca gttatcgcgg cttagtgaaa atcatgccga
     1741 cggcaaccaa tgcgcgcaat ttcactcagt gcgactcaat gctgattggc gctaattgtg
     1801 gggcgcatac cttcccgtat gtcgaatgtc gcaataacag cgcacaactg gagcacgagg
     1861 caacgacatc acgtattggt gaagatcaac tgttttactg tctgcaacgc gggatcagcg
     1921 aagaagacgc catctcgatg attgttaacg gtttctgcaa agacgtgttc tcagaactgc
     1981 cgctggaatt tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa cacagcgtcg
     2041 gataaggaat aaacatgtta agtattaaag atttacacgt cagcgtggaa gataaagcta
     2101 tcctgcgtgg attaagcctc gccgttcgtc ccggcgaagt tcacgccatt atggggccaa
     2161 acggttcggg caaaagtacc ttatcggcaa cgcttgccgg gcgcgaagat tatgaagtga
     2221 cgggcggcac ggttgagttc aaaggcaaag atttgcttgc actgtcgccg gaagatcgtg
     2281 caggcgaagg catctttatg gccttccagt atccggtgga gattccaggc gtcagtaacc
     2341 agtttttcct gcaaacggca cttaatgcag tgcgcagcta tcgcggccag gaaacgctcg
     2401 accgctttga ttttcaggat ttgatggaag agaaaatcgc tctcctgaag atgccggaag
     2461 atttattaac ccgttcggta aacgttggtt tttccggcgg cgagaaaaag cgcaacgata
     2521 ttttgcaaat ggcggtgctg gaaccggagt tatgcattct tgatgagtcg gactccgggc
     2581 tggatattga tgcattaaaa gtggttgccg atggcgtgaa ctcgctgcgt gacggcaagc
     2641 gctcattcat cattgttacg cactaccaac gcattctcga ctacatcaag cctgattacg
     2701 tccatgtgct atatcaggga cgaattgtga aatccggcga tttcacgttg gtcaaacaac
     2761 tggaggagca gggttatggc tggcttaccg aacagcagta acgcgctgca acagtggcat
     2821 cacttgtttg aagctgaagg gacaaaacgc tccccgcaag cacagcagca tttacaacaa
     2881 ttgctgcgta ccggactgcc gacacgtaaa catgaaaact ggaaatatac gccgctggaa
     2941 gggctgacca atagccagtt tgtcagcatt gcgggggaga tatccccaca gcagcgtgat
     3001 gccttagcgt taacgttaga cgccgtgcgg ctggtgtttg tcgatggacg ttacgtgccc
     3061 gcgctgagcg atgcaattga aggcagcgga tatgaagtga gcattaacga cgaccgtcag
     3121 gggttacccg acgctattca ggcggaagtg tttctgcatt tgacggaaag cctggcacaa
     3181 agcgtgacgc atatcgccgt gaagcgcggt caacggccgg caaagccatt gctgttaatg
     3241 tatatcaccc agggcgtggc aggtgaagag gtgaacactg cccattaccg gcatcatctg
     3301 gatctggcgg aaggtgccga agcaacggtg atcgaacatt ttgtcagcct taatgatgct
     3361 cgtcatttta ccggggcacg gttcactatc aatgtcgcag cgaacgccca cttgcagcat
     3421 atcaagctgg cgtttgaaaa cccgctcagt caccactttg ctcataacga tttgttgctg
     3481 gctgaggatg ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt gttacgacac
     3541 aacaccagta cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag cctggcgatg
     3601 ccggtgaaaa acgaggtgtg tgatacccgt acctggctgg aacacaataa aggtttttgt
     3661 aacagccgac agttgcacaa aactatcgtc agcgacaaag gccgcgcggt atttaacggt
     3721 ttgatcaacg tcgcgcagca cgccatcaaa acggatggtc agatgaccaa caataatctg
     3781 ctgatgggca aactggcgga agtggatacg aaaccgcagc tggaaatcta tgcagatgat
     3841 gtgaaatgca gccacggcgc gacggtgggg cgtattgatg atgaacagat gttctatctg
     3901 cgctcgcgcg ggatcaatca gcaggatgcc cagcagatga tcatttacgc ctttgctgcc
     3961 aaactgacgg aagcactgcg tgatgagggg cttaaacagc aggtgctggc ccgaatcggt
     4021 caacggctgc caggaggtgc aagatgactt tttccgtcga caaagtgcgg gccgactttc
     4081 cggtgctttc gcgtgaggta aacggtttgc cgctggctta tctcgacagc gccgccagtg
     4141 cacagaaacc gagccaggtg attgacgccg aggccgagtt ttatcgtcat ggctacgcgg
     4201 cggtgcatcg cggtattcat accttaagcg cccaggcgac cgagaaaatg gagaacgtgc
     4261 gcaagcgggc atcgctgttt attaatgccc gttcggcgga agagctggtg ttcgtccgcg
     4321 gcacgacgga agggatcaat ctggtcgcca atagctgggg caacagcaat gtgcgggcgg
     4381 gcgataacat catcatcagt cagatggagc accacgctaa cattgtcccc tggcagatgc
     4441 tttgcgcacg cgttggcgca gagctgcgtg tgatccctct caaccccgac ggtacgctgc
     4501 aactggagac gctgcctacg ctgtttgatg agaaaactcg cctgctggca attactcatg
     4561 tctccaacgt gcttggcaca gataatccac tggcggaaat gatcacgctt gcgcaccagc
     4621 atggcgcaaa agtgctggtg gatggcgctc aggcggtgat gcatcatccg gtggatgttc
     4681 aggcgctgga ttgcgacttt tacgtgttct ccgggcataa actgtatggc cccaccggaa
     4741 ttggcattct ttatgtcaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg
     4801 gttctatgat cgccaccgtc agcctgagtg aaggcactac ctggaccaaa gcaccatggc
     4861 ggtttgaagc cggtacaccc aataccgggg gcatcattgg tcttggcgcg gcgctggagt
     4921 atgtttcggc gctggggctt aatagcatag ccgagtatga acagaatctg atgcattatg
     4981 cgctatcaca gctggaatct gtaccggatc tcactctcta tggcccacaa aacaggcttg
     5041 gcgttattgc ttttaatctc ggtaaacacc acgcctatga tgttggcagt tttctcgata
     5101 attacggcat tgctgtgcgt accggacatc actgcgctat gccattaatg gcctattaca
     5161 acgtccctgc gatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa gaagtggatc
     5221 gtctggtgac cggcctgcaa cgtattcacc gtctgctggg ataacaggga ggcactatgg
     5281 ctttattgcc ggataaagaa aaattgctgc gtaatttttt acgctgtgcc aactgggaag
     5341 agaaatatct ctacattatt gagctgggcc agagtctgcc agaattacgc gccgaagacc
     5401 gaagcccaca aaatagcatt cagggctgcc agagtcaggt gtggattgtc atgcgccaga
     5461 atgcgcaggg aattattgaa ttgcagggcg acagcgatgc ggcgattgtg aaagggctta
     5521 ttgcggtcgt ctttattctc tacgatcaga tgacgccgca ggatattgtc aatttcgatg
     5581 tgcgtccgtg gtttgaaaaa atggcgctca cccaacatct caccccatct cgttcacaag
     5641 gtctggaagc gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag
     5701 ggacagcttt catccggttg gctcacattg ccggatggtc aatgtttaca ctgacctgca
     5761 caggagtgtt ttggtttcag gtgaacataa gcgccatccg acgtttcagc gtgagtctcc
     5821 ggcaaataca ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc
     5881 gcttttagcg ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggaagc
     5941 cgactggttg ggcaaaatca aacgtatacg gtgcaagaag gggataaaaa ccttcaggct
     6001 attgcccgac gttttgatac tgcggcaatg ttgatccttg aagccaataa cactatcgcc
     6061 ccggtgccaa aacctggtac gacgataact attccttcac aactgttatt acctgatgca
     6121 ccgcgtcagg ggattatcgt taaccttgca gagctgcgcc tttattatta tccgccggga
     6181 gaaaatattg tgcaggttta tccaataggt attggattgc aggggctgga aacgccggtg
     6241 atggaaacgc gtgttgggca gaaaatccct aacccaacct ggacgcctac ggcaggcatt
     6301 cgtcagcgtt cgctggagcg tggcattaaa ttaccgccag tcgttcctgc cggaccaaat
     6361 aacccgctag gacgttacgc actgcgcctc gcgcatggta atggcgaata cctcattcat
     6421 ggcaccagtg cgccggacag cgttggtttg cgcgtcagtt cagggtgtat tcgcatgaat
     6481 gcaccggata ttaaagcctt gttctccagc gtgcggacgg gaacgccggt gaaagtgatc
     6541 aacgaaccgg tgaaatattc cgtggaacct aacgggatgc gttatgttga agtacatcga
     6601 ccactatcag cagaagaaca gcaaaacgtt cagacaatgc catacacact gccagcaggc
     6661 tttacgcaat ttaaagacaa taaggctgta gatcagaagt tagtcgataa agcgttgtat
     6721 cgtcgggcag ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc cagcaatgcg
     6781 ccttcagtag agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt acgcgcgacg
     6841 cagtagcggt aaacggcaga caaaaaaaat ggcgcacaat gtgcgccatt tttcacttca
     6901 caggtactat tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc
     6961 tgcgtcatct ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg
     7021 gtcaacttta gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct
     7081 ggagcaacct gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc
     7141 tttcattatt aataccctct agattgagtt aatctccatg tagcgttaca agtattacac
     7201 aaagtttttt atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc
     7261 tcaaagaagc atcgaacgct gggttttaga taaaaaaatt gagagaaaac aggatgcttc
     7321 catcggattc atcttagata aagtgagatt atatagtgaa atccgatttg attttttatt
     7381 gcttctggtt
//
LOCUS       DAJSKJ010000175         7324 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000175 DAJSKJ010000000
VERSION     DAJSKJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.177"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            184..1248
                     /gene="ulaG"
                     /locus_tag="ON345_003319"
     CDS             184..1248
                     /gene="ulaG"
                     /locus_tag="ON345_003319"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR3073331.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1356..2111
                     /gene="ulaR"
                     /locus_tag="ON345_003320"
     CDS             1356..2111
                     /gene="ulaR"
                     /locus_tag="ON345_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR3073332.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGQNANPEILQQLEAQGVSILRV"
     gene            complement(2108..2857)
                     /gene="yjfP"
                     /locus_tag="ON345_003321"
     CDS             complement(2108..2857)
                     /gene="yjfP"
                     /locus_tag="ON345_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3073333.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDALDHGSRFRGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLADRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTLLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3039..3368
                     /gene="bsmA"
                     /locus_tag="ON345_003322"
     CDS             3039..3368
                     /gene="bsmA"
                     /locus_tag="ON345_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR3073334.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3517..3792
                     /gene="yjfN"
                     /locus_tag="ON345_003323"
     CDS             3517..3792
                     /gene="yjfN"
                     /locus_tag="ON345_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR3073335.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3909..5534)
                     /locus_tag="ON345_003324"
     CDS             complement(3909..5534)
                     /locus_tag="ON345_003324"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR3073336.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGWRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5618..6781)
                     /locus_tag="ON345_003325"
     CDS             complement(5618..6781)
                     /locus_tag="ON345_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR3073337.1"
                     /translation="MLRHNVPVRWDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6784..7275)
                     /locus_tag="ON345_003326"
                     /pseudo
     CDS             complement(6784..7275)
                     /locus_tag="ON345_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
BASE COUNT         1923 a         1952 c         1787 g         1662 t
ORIGIN      
        1 atttaaattc atttagttaa ttgaattaca cattccatta aatctttcca cactcaaaat
       61 caacaacaac tttcctggca aaaagtcaaa aaatggtcat tgccttcctg aaaaaataga
      121 gtcagattaa tcaaaacaaa tcacatgttg attcgaattg aactatcatg aaggtaaatg
      181 gcgatgagta aagtgaaaag tatcacccga gaatcctgga tcctgagcac attcccggag
      241 tggggtagct ggctgaatga agaaattgaa caagagcagg tcgcccctgg cacatttgcg
      301 atgtggtggc taggctgcac cgggatctgg ctgaaatcag aaggtggcac caacgtttgc
      361 gttgatttct ggtgcggcac tggcaaacaa agtcacggta acccgttaat gaaacagggt
      421 caccagatgc agcgcatggc tggcgtgaaa aaactgcagc caaacctgcg caccaccccg
      481 tttgttcttg atccgtttgc gattcgccag atcgacgcgg tactggcgac tcacgatcac
      541 aacgatcata tcgacgttaa cgtcgctgct gccgtgatgc agaactgtgc tgatgacgta
      601 ccgttcattg gaccgaaaac ctgcgttgat ttgtggattg gctggggcgt accgaaagag
      661 cgttgcatcg tggtcaaacc gggcgatgtt gtaaaagtga aagacattga aattcatgcg
      721 cttgacgctt tcgaccgtac tgcactgatc accctacctg ccgatcaaaa agcggctggc
      781 gtactgccag atggcatgga cgatcgcgca gtaaactacc tgttcaaaac ccctggcggt
      841 tccctgtatc acagtggcga ttcccactac tctaactatt atgcgaagca cggtaacgaa
      901 catcagatcg atgtggcgtt aggatcgtac ggtgagaacc cgcgcggtat taccgacaaa
      961 atgaccagcg ccgatatgct gcgtatgggt gaagccctga atgcgaaagt ggttatcccg
     1021 ttccaccacg acatctggtc aaacttccag gccgatccgc aagagatccg cgtgctgtgg
     1081 gagatgaaaa aagatcgtct gaagtatggc ttcaagccgt ttatctggca ggtaggcggc
     1141 aaatttacct ggccgttgga taaagacaac ttcgagtacc actatccgcg tggtttcgat
     1201 gattgcttca ctattgaacc ggatctgccg ttcaagtcat tcctgtaatc tgtcgtttat
     1261 accggatagc gtggagtagt tcgcgctatc cggcgtagta tttcctggct atttcaaata
     1321 tcatctaaaa aaatcaaatt ttatcggaat agctcatgac tgaagcacaa agacatcaaa
     1381 tcctcctgga aatgctcgca caattgggct ttgtgaccgt tgagaaagtc gttgagcgtc
     1441 tgggaatttc gcctgccact gcgcgacgcg atatcaataa acttgacgaa agcggcaaac
     1501 tgaaaaaagt gcgcaatggc gcagaagcta ttacccagca gcgcccacgc tggacgccga
     1561 tgaatctgca tcaggcgcag aatcacgatg aaaaagtacg tattgctaaa gcggcctcgc
     1621 agctggttaa tccgggcgaa agcgtagtca tcaactgcgg ctccaccgcg tttctgcttg
     1681 ggcgggaaat gtgtggcaag ccagtgcaaa tcatcactaa ttatctaccg ctggcaaatt
     1741 acctgatcga tcaggagcat gacagcgtga tcattatggg cggacagtac aacaaaagtc
     1801 agtccatcac tttaagcccg caaggcagcg aaaacagtct ctatgccggg cactggatgt
     1861 ttaccagcgg caaagggctg acagcagaag ggttgtataa aaccgatatg ctgacggcaa
     1921 tggcagagca gaagatgcta agcgtggtag ggaaactggt ggtactggtt gatagcagta
     1981 agatcggcga acgcgcggga atgcttttta gccgtgccga tcaaatcgat atgctgatca
     2041 ccggccaaaa tgctaacccg gaaatcctgc aacaactgga agcgcaaggg gtcagcattc
     2101 tgcgtgttta aagatgctgg cggaaaaatg tcacggcagc atctaacgcc tcaggcgtaa
     2161 tgcggtgacg cacgcctggc tgccatgaac aggttagcag tttatcccgt cccgtctcgc
     2221 ttaaggcctg ctgcaaacgt agtgattcgt cggcaggcac gacgtcatcg tcaaggccat
     2281 gccacagcag tagaggtctg tcagcaagtt gttccaggtg gtttgtcgct tcccactctg
     2341 ccagcggcgc gacaatgtta ttgaattcat tctgctgtgc tgccgtttca ggtatcagcg
     2401 gtggaaacag tgaacgggcg agcaatgtaa aatagcccga tcccatcatg ctggcggtac
     2461 atttcaccgt ggggtggcga gcggtaattc ccagtgccgt catcgcgccc atcgacgcgc
     2521 caccgactgc cagacggtca gcaagcagcc agttttcttc ggctattgcc gcacgtaacg
     2581 tagtgaattc ctgcatactt tgtagcaaaa tttgccaaaa ttgatttaac cgccgcgctg
     2641 cgtcgcccct aaaacggcta ccgtgatcga gcgcatccgg catgatcact cgcaaaccag
     2701 cctgcgccag cgcaacggca aaatagctat acaccagact ggacgaagta aagccgtgat
     2761 aaaaaatcac gcacggtaat ggggtatctt tttgcccgac aggataagca tgaagaacgg
     2821 gaatatctgc cagctcgcgt gattctattt caatcatctg tctctccttt ttgtctggta
     2881 tgacatgaag aaatccttag cacaagtttc tgctggcggt attagttcat aatgttgaga
     2941 tgtgggttac gctttcgtca cgtattcatt cgaaaatcgc gtaccaggta acaattcggg
     3001 aacattcccc caaagttaaa ttaataaggc actacactat ggttagcagg aaacgtaata
     3061 gcgttattta ccggtttgcc agtttattat tggtgttgat gttaagtgcc tgtagcgcac
     3121 tgcaaggtac gccacagcca gcaccgccag taaccgacca tccgcaagag attcgccgcg
     3181 accagacgca agggttacag cgaataggta gcgtaagcac gatggttcgg ggttctccgg
     3241 atgacgcatt agcagaaatc agagcgaaag ctgtcgctgc aaaagctgat tattacgttg
     3301 tcgtcatggt ggacgaaacc atcgtgacag gacagtggta ttcacaagcc attttgtacc
     3361 gtaaataaat cagacaactt acatagtcag ctttacattg ctttgcgtcc ctatgcgttt
     3421 acctgtgcac aataaattac cggctggaag cctgggcgtt tattaccgcg atggaatgcc
     3481 ctgcaacatg tggggaaacg aaaaatggag ctgacgatga aacaattact tgcctcaccc
     3541 tcgctgcaat tagtgactta tcctgcgagc gccacggcac agtctgcaga attcgccagt
     3601 gctgactgcg tgacgggcct gaatgaaata ggtcagatct cagttagcaa tatttcaggg
     3661 gatccgcagg acgtggaacg tatcgtggcg ttaaaggccg atgaacaagg tgcgtcatgg
     3721 taccgcatta ttaccatgta cgaagaccag cagcctgata actggcgcgt acaggcgata
     3781 ctgtatgcat gagtgtcatc tacagcaaat gaacaatgcc ggatgcggcg cgagcgcctt
     3841 gtccggccta caattttcac atatttcatt gagttaattg aaaccagcgt tgcatcagtc
     3901 gtatacgctt acacacacac tccccccgtc gcccgcagca ataaatcatt ctggatctgc
     3961 tctgacaacc gtacgccgcc gcgcgtatct aacatcacct gacaccacgc ctgcgccatt
     4021 ggcggagagg catatttcaa catttgcgca ccgcagccca gcaggaatag ctgatgagta
     4081 atctctcgcc ccagttcttc agcaggctta cgcagctgct gctgtaaacg acgaaccgcg
     4141 cgatcaaaat agcgatcctg ccctttcact tccacaaatg cttccgacaa taagtcgtat
     4201 acgcccgctt gcttattgag aacgcgcagc acatccaggc acataatatt gccggaacct
     4261 tcccaaatac tgtttaccgg catctcccgg taaagccgcg gcaactcgct ctcctcgcaa
     4321 taaccaatgc cgcccagcac ctccatcgct tcggccacaa acggcatacc tcgtttgcag
     4381 atcacaaatt tcgccgcagg cgtaaataaa cgcgcccaca gggcttcttt ggcatcggca
     4441 cgccggtccc acgctcgcgc aagacgaaac agcaacgccg tttgcccttc aagctgaagt
     4501 gccatgcgac ttaagacatg acgcataagg ggctgttgga tcaatggatt accaaaaaca
     4561 tggcgttgat gtgcatgata aatcgctagc gaaaatgcac ggcgcatcat ggcatggcta
     4621 cccagggcgc aatcaaaacg cgtcatccca cccattttca ggatcagacg aattccttcc
     4681 ccttcctgcc ccaacaacca gccaatggca tcctgaaact ccacttcgca gctggcgtta
     4741 gagcgattac ccagcttatc tttcagccgc tcgaggcgaa tcgcgttgcg ttgcccgtca
     4801 ggcaaaaagc gtggcacaaa aaagcaggac aaaccgcccg tagtctgcgc cagcaccaga
     4861 tgcgcatcgc tttgcggcac cgagaaaaac catttatgcc ccaccagccg ataagagcca
     4921 tcttccagac gctctgcgcg ggtggtgttg ctcatgacat cggaaccgcc ctgcttttcc
     4981 gtcattccca tgccaatcaa caaaccgcgt ttttgcccac ctggcaataa gtgagaatcg
     5041 tagcgatcgc tcaacagcgg cgtggtccag tcctgaaacg gcgtgggtaa catctgtaac
     5101 aacaatggcg tggcggcaaa ggtcatcgtt atcggacata acgaccctgc ctcaacctgt
     5161 gcgtgcaaca taaaacgcgc cgcgcgcgcc acaaatgcgc cggagcgagc gtcttcttcc
     5221 caggccagat tatgtacccg attggtacat agcgcctgca tcagcaggtg ccaggcaggg
     5281 tgaaaacgca catcgtccag acgccacccc tgggcatcgt agcgcaataa ttcaggcgga
     5341 ttcacattcg ccagccgccc cagttcaagg gattcagccg ttcctaactg ctgaccaata
     5401 ctggctagaa aatcgctatc ccagccagca ccttcacgcg ttaccgcttc gcagagcgcg
     5461 ccatcagaca ggtataagtt gctgttattt aatggtatag gttgattaaa aacggtgtga
     5521 gtttgccagt gcactgtgtc tccctccatc aatggcagtc accattagta tggtcactgc
     5581 catggattca tgacagaaag agattgctaa aacattctta tccagcaatg taatgcggaa
     5641 cgaaacgtga ggtgtctttg gtgatcagcg tgttatcttc acggatcccc attccgcacg
     5701 cttcatcatc gacaatccag ctaccgatga gtgtgtagct atcgccaaat cgcggcagag
     5761 gttgaaacgc ctggtagatc atcggttcat cggcgtaatc accatcagcg tggtcaacga
     5821 cgttattctg accgtcgaaa atggtgacgt ttccgccttc gcgtgagtag atcggtttac
     5881 gcacatagct ttcgccagcg gcaatctgcg gtttttcgcc ctcaaaccag gatgcaagaa
     5941 ggttaggatg accaggaaag aagcgccaaa gcagcggcat taaccctttg ttgctcaaga
     6001 tacttttcca taacggttcc acccattgct cgcggcgctt gcgcagcagc ggaccgttat
     6061 cgtcacgcat catccactcc agcggataca gcttaaatgc acgctggatg acattatcat
     6121 caagatcggt cagtacaccg ccgacgccca aaccgagatc ttcaatgtag ataaaccgcg
     6181 actcctgccc tgcctgctgg gcgcagtcct gcaagtacag cacggtactc ctgtcttcat
     6241 ccgtgtcctg acagcagcaa aaataaaacg gttcccgact gtaaagctca ctaaagcgcg
     6301 aaatcaggcg ttcctgaatg gcattgtact gatcggcatc acgcggaata atgccgctgc
     6361 gccgggcatc ttccagccac agccactgga aataagccga ttcatacaac gaggttggcg
     6421 tatcggcgtt gtactccagc agcttcaccg gcgcattgcc acaccaggca aaatccatcc
     6481 ggccatagag cgaaggatcg cgtgcgcgcc agctttcggc gatcacatcc cagtacaacg
     6541 gcgggatcgc cagttgcgtc aggatctctt cgtctttaac cgcgcgatcc accacctcaa
     6601 ggcacatctg atgcagttcc gcagttggtt tttcgatctg ctcttcaatc tgacgcaggg
     6661 taaagcggta agcccggctc tcatcccaat agatttcatt gtcgatgata tgaaagtcga
     6721 aaccgttgtc agcggcgatc tggtccagat cccaccgtac aggaacgttg tgtctcagca
     6781 tgattagcct ccccaatgcc cacgggcgct ggaagaacga ccatagccgc cgcgagaaac
     6841 ggttgacgct tttttggtgg taaagccgcc cgaagaatag gactcttttt tgccagaacc
     6901 ggagcgccag gagtaatcac cggaagtgtt gcgccagacg gggcgcgaag caaaagagga
     6961 accgccgctg ttatacacaa actgctcgtc gcgatcttta cgaataaccc ggcttaacaa
     7021 aaatccagaa acaaccggga tccaactctg ttcaacgttg tcgtagtaac aattttcata
     7081 cttagactga caattctgct gagtcatatt cttcggaaca tcggcataaa acgccgcttt
     7141 ggcgttgttc cagccatgag cacagatatc tgaattatta ccgtcatcaa tacaatcctg
     7201 cacagtcgag taaaacgttc cgtcgccgtc gttatcgaca tcgccactat cgccacaacc
     7261 tttcaatacg aaaaaaggta atgactccaa cttattgata gtgttttatg ttcagataat
     7321 gccc
//
LOCUS       DAJSKJ010000176         7301 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000176 DAJSKJ010000000
VERSION     DAJSKJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.178"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(23..1447)
                     /gene="ascB"
                     /locus_tag="ON345_003327"
     CDS             complement(23..1447)
                     /gene="ascB"
                     /locus_tag="ON345_003327"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR3073338.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(1456..2913)
                     /gene="ascF"
                     /locus_tag="ON345_003328"
     CDS             complement(1456..2913)
                     /gene="ascF"
                     /locus_tag="ON345_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR3073339.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            3173..4183
                     /gene="ascG"
                     /locus_tag="ON345_003329"
     CDS             3173..4183
                     /gene="ascG"
                     /locus_tag="ON345_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR3073340.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            4332..4859
                     /gene="hydN"
                     /locus_tag="ON345_003330"
     CDS             4332..4859
                     /gene="hydN"
                     /locus_tag="ON345_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR3073341.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            5012..7264
                     /gene="hypF"
                     /locus_tag="ON345_003331"
     CDS             5012..7264
                     /gene="hypF"
                     /locus_tag="ON345_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR3073342.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLYQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAAFMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
BASE COUNT         1665 a         1916 c         2037 g         1683 t
ORIGIN      
        1 acgccgcatc cggcactgtt gcttactcta aatcttcacc attactggca atcacttttt
       61 tataccacca gaacgatttc ttacgtgtgc gcgtcagcgt gccgttgcct gcgtcgtcac
      121 ggtctacgta gacaaaaccg tagcgtttgc tcatttcacc cgtagaggcg gaaactaaat
      181 caatacagcc ccatgtggtg tagcccatca gcggaatgcc atctgcaatc gcttcgccca
      241 ttgcgcggat atgttcgcgt aaatagctga tgcgatagtc atcgttaatc tcgccattgg
      301 cagcaagttc atcttttgcg ccgaggccgt tttccaccag aaacagcggc ttctgataac
      361 ggtcgtacat catgttcatg gtgatacgca gaccgagtgg atcaataccc cagccccagt
      421 cgctcacttg cagatacggg ttacgcagcg atttcactac gttcgccgca ctgctattgt
      481 tggcgttcat ctccgccgag gcgcagcgcg aggcgtaata gctgaaagag acaaaatcga
      541 cggtgttttt caggatttca tcatcgcccg gtgctttgtc gatggttacc cctttttcgc
      601 ggaatacgcg ggcagagtaa gccggatacg tgccccgcgc ctgcacatcg ataaaaaaca
      661 ggttttcccg gtctttctcc agcgccgccc agacatcttc cggcttgcaa ctgtaagggt
      721 agaagttacc gcccgccagc atacagccca cctgattttg cgggttaacc tcatgggcga
      781 ttttggtggc tagcgcactg gcaaccagct ggtgatgcgc ggcctgatat ttcacctgat
      841 cctgattttc accttcttca aacaccagac ccgcgccgga gaacgggcta tgcaacataa
      901 tgttgatttc attgaaggta agccagtatt tcaccagacc atcaaatgct tcaaagcagg
      961 tccgggcgta gcggctgaaa aactccacca gcttgcggtt acgccaggag ccatattcgg
     1021 tgaccagatg catcggcaca tcgaagtggc acaacgtgac cagcggttcg ataccgtact
     1081 ttttacactc ttcaaagaca gaacgataaa aagcaatgcc ctgctgattg ggcgtgattt
     1141 catcgccctg cggaaagaga cggctccagg caattgaggt acggaaaacc ttgaatccca
     1201 tctctgccat cagggcgata tcttctttat aacgatgata aaaatccgtc gcctcatggc
     1261 tgggataaaa ctcgtcatcg cgcaactgaa aacgtttttc cagccccagt ttcaccgcca
     1321 ttcgatgctc gccgtgtggg atcatatcga cagtggtcag ccctttgcca ccttcacgga
     1381 acgcaccttc agactggttg gcggcaagcg cgccgcccca taaaaaacct tctggaaata
     1441 ctgacatttt catcctcaat taagacttac ttctttggcg acggtcggtt gtacgctctg
     1501 atgctttcgc gcctcggcag tcgcttcctc aacaggaata tcctcaaagc cgagcaacag
     1561 tgtgaggata aacgacagca cgaccgccag cgccatgacc gcgaacaccc agacgatgct
     1621 cattggattc gccggatcga agaactgcac gctggtaaat agccccggcg ctgccattga
     1681 gtggctggca agccccgcca taccggcaac cgcgccgcaa ataaagccgc tgataagact
     1741 ggcgataagc ggacgtttca ggcggatcgc cacgccgtat aacgccggtt cggaaatccc
     1801 cgccataatg gctgatgccg ccgcagccag cgccgtctgg cgcagttccg ggtttttcgt
     1861 tttccacgcc accgccagtg atgaaccgcc cagcgacagg ttagcgccga tctctgacgg
     1921 catgaccatc ccttctttgc cggtttcggc aatggtctga atgatggttg gcgtaaagac
     1981 gcggtgcatc ccggtcatta ccagcagagg ccacagcgcg cccataatgg cgactgaaag
     2041 ccagcccaga taaccatgaa tggtgtaaac cagcgccgaa atggcgctac cgatccagat
     2101 accaatcggg ccaatcagca ggattgccag cggtgcggca atcaacacaa tcagcattgg
     2161 cttgaggaag tttttggtca ccgccggtgt aatgcgatcc acccagcgtt cgatatatga
     2221 caggcaccag gtcatcacca gcgccgggat cactgtgtag gtgtatttca ccgcggtgac
     2281 cggaatcagg gcaaattcga catgttcacc ctgggccgct ttcgccatca gttcaataaa
     2341 actcggatgt accagcacac ccgcgatggc aatcgccagc gacatattgg ttttaaattt
     2401 gatggcggca gatgccgcga ccatcagcgg caggaagaag aaagcaccgt caccaatcac
     2461 attcagaatg gttaaggtcg gcgatccttt tgtcagcacg ccgctcatct cgaggatcat
     2521 cgccagcagt ttgaccatcg atccgccgat aatcgccggg atcagcggtg acatggtgcc
     2581 gatcagcgca tcgaggatcc ccgcaccaat gcgacgcagc gtgagtttgg gtttacccac
     2641 gggctgtgcg ggctgcatat ctcccggcag caggctgacg acttcctgaa aggcttgtga
     2701 aacggtattg ccgataatca cctgacactg gttgtcacta cgtaccacgc cgagcacgcc
     2761 ggggatggtt tttaacgtcg ggctgtcgat aagctggtca tctttgataa caaagcgcaa
     2821 acgcgtcata cagtgcgtga ccgccgagat gttatcaacg ccgcccagtg ccgctatcac
     2881 cgagcgtgcc agcgccgcat aatttttggc catcggattt tatcctgtta tcagtagggt
     2941 agatgtagtt gttatcagtc gacgtaacgg aaaaatgccg atgctgaaac ggaattagga
     3001 aaccggtttc acaaaatcat gaatagttgt cactaaataa gcaagagagt taattgttca
     3061 ttttttatat ttgtgaaacc ggtcacctga atatttttcc cgccgattgc gcaaggacaa
     3121 tcggcaaacg gtgcagtaca ctgcgttgat tgcgtgtaaa gggatgaaaa tgatgacgac
     3181 gatgctggaa gtggcgaagc gcgccggggt ttcaaaagcg accgtttccc gcgtgctttc
     3241 aggtaatggc tacgtcagcc aggagactaa agatcgcgtg tttcaggcgg tagaagagag
     3301 cggttaccgc ccaaacttgc tggcgcgcaa tctgtcggcg aagagtactc agacgctggg
     3361 gctggtagtg accaacacgc tttaccacgg catttatttt agtgaattac tgtttcatgc
     3421 cgcgcgaatg gcggaagaga aagggcggca gttgctattg gcagatggta aacacagcgc
     3481 cgaagaagag cgccaggcga ttcagtatct gctggatttg cgctgcgacg ccattatgat
     3541 ttacccgcgt tttttaagcg tggatgagat cgatgacatc attgacgcgc acagtcagcc
     3601 gataatggtg cttaatcgcc gcctgcgcaa aaacagcagc catagcgtct ggtgcgatca
     3661 taaacagacc agctttaatg ccgtggcaga gttgataaac gccggacatc aggagattgc
     3721 tttccttacc ggctcgatgg attcccccac cagcattgaa cgtcttgctg ggtataaaga
     3781 cgcgctggcg cagcatggta ttgcgctcaa tgaaaaactt atcgctaacg gtaaatggac
     3841 gcccgccagc ggggccgaag gggtagaaac gttgctcgaa cgtggggcta aatttagcgc
     3901 gttagttgcc agtaacgacg atatggcgat aggtgcgatg aaagcgttgc acgagcgcgg
     3961 cgtagcggtg ccagagcagg tgtcagttat cggattcgat gatatcgcaa ttgcccccta
     4021 tatcgttccg gcgctctcca gcgtaaaaat tccggtgact gagatgattc aggaaattat
     4081 tggacggctg atttttatgc tcgatggcgg ggatttctca ccgccgaaaa ccttcagcgg
     4141 aaaactgatc caccgcgact cactcattgc tctttcgcga taaacattgt catcacatgt
     4201 catcgtcact agtgtcgatg acatcttttt tcctgcatta cagaaattaa tttccttaaa
     4261 taacagtaaa ttaaaaactg gcatgatttg tgaatgtatc ggcgcattaa ctgtcattgc
     4321 tggagaattt gatgaaccgt ttcatcattg ctgacgcgag taaatgtatt ggttgccgta
     4381 cctgtgaagt ggcgtgcgtg gtttctcatc aggaaaatca ggactgtgca tcgctgaccc
     4441 cggaaacatt tttaccgcgt atccatgtca ttaaaggtgt gaacatttcc acggcgacag
     4501 tttgccgtca gtgtgaagat gcaccgtgcg ctaacgtctg cccgaatggt gctatcagcc
     4561 gtgataaagg gtttgttcat gtgatgcagg aacgttgcat tggttgcaaa acctgcgttg
     4621 tggcttgccc gtatggcgcg atggaagtgg tggtacgtcc ggtgattcgc aacagcggcg
     4681 cggggctgaa tgtacgggct gacaaagccg aagccaataa atgtgacctg tgcaaccatc
     4741 gtgaagacgg cccggcgtgt atggcggctt gcccgactca tgcgctgatt tgtgtcgatc
     4801 gtaataaact tgaacaactg agtgcagaaa aacgccgccg tacggcgctg atgttctaaa
     4861 aatcttacag aactgttctg gcatcaaagg aattcccgga aatattcgta gaatacggag
     4921 cgggcggtat cgccgacctg tgactgccgg atgcgacgct gtcgtgtccg gctgggcggg
     4981 ttacgcaatt catgatggcg ggaataactc aatggcaaaa aacacatctt gcggtgtcca
     5041 actgcgtatt cgtggcaaag tgcagggcgt cggttttcgt ccgtttgtct ggcagctggc
     5101 acagcaatta aatcttcacg gcgatgtctg taatgacggc gatggcgtag aagtccggct
     5161 gcgggaagac ccggaaacgt ttcttgttca attgtatcag cactgcccgc cgctggcgcg
     5221 tattgatagc gtcgagcgtg agccgtttat ctggtcacaa ctgcccaccg agttcactat
     5281 acgccagagc acaggcggca ccatgaatac gcaaattgtt cccgatgccg ctacttgccc
     5341 tgcttgcctt gccgaaatga ataccccagg cgaacggcgt tatcgttatc cgtttatcaa
     5401 ctgtacccac tgcggcccgc gtttcaccat tattcgcgcc atgccttacg accgcccgtt
     5461 taccgtgatg gcggcgtttc cgctatgtcc ggcctgtgac aaagagtacc gtgacccgct
     5521 cgatcgtcgc ttccacgccc agccggtggc ctgcccggag tgtggcccgc atcttgaatg
     5581 ggtaagtcat ggtgaacatg cggaacaaga ggcggcatta caggcagcta tcgcacagtt
     5641 aaaaatgggc aaaattgtcg ccatcaaagg gattggcgga tttcatcttg cctgcgatgc
     5701 acgtaacagt aacgcggtgg cgacactgcg ggagcgcaaa caccgcccgg cgaaaccgct
     5761 ggcagtcatg ttgccagtag cagaaagttt accagacgct gcgcgccagt tgcttaccac
     5821 gcccgctgcg ccgattgtgc tggtggataa aaaatacgta cctgagcttt gtgatgatat
     5881 cgcccctggc cttaacgaag tcggagtgat gttgcctgcg aacccgctcc agcatttgct
     5941 gttacaggaa ctgcaatgcc cgctggtgat gacctccggc aacctgagcg gtaaaccacc
     6001 ggctatcagc aacgaacagg cgctggcgga tttgcagggc attgccgacg gattcttgat
     6061 acataaccgc gacatcgtgc agcggatgga tgattcggtg gtgcgcgaaa gcggcgaaat
     6121 gctgcgccgt tcgcgggggt atgtgccgga tgcgctggct ttgcctccgg gctttaaaaa
     6181 tgttccgcct gtgctgtgtc tcggcgcgga tctgaaaaac accttctgcc tggtgcgcgg
     6241 tgaacaagcg gtgttgagtc agcatctggg cgatttaagt gacgatggca tccagatgca
     6301 gtggcgcgaa gcattacgcc tgatgcaaaa catctacgat ttcactccgc aatacgttgt
     6361 gcatgacgcg catccgggct atgtctctag ccagtgggcg cgcgaaatga atctgccgac
     6421 gcaaacggtg ctgcatcatc atgcccacgc agcggcgtgt ctggcagagc atcagtggcc
     6481 gctggatggc ggtgatgtca ttgctttgac gctcgacggt atcggtatgg gggagaacgg
     6541 cgctttgtgg ggcggcgagt gcctgcgggt gaactatcgc gaatgccagc acctgggcgg
     6601 cttgcctgcg gtggcgcttc cgggtggcga tttggcagcg aagcagccgt ggcgaaacct
     6661 gctggcgcag tgcctgcgct ttgtgccgga gtggcagaat taccctgaaa cagcaagtgt
     6721 gcaacagcaa aactggagcg tgctggcgcg ggccattgag cgtggaatta acgcgccgct
     6781 ggcgtcatcg tgtgggcggt tgttcgatgc agtggcggcg gcactgggct gtgcgccagc
     6841 cacgttaagt tatgaaggtg aagcggcttg tgctctggag gcgctcgcag cctcatgcca
     6901 cggagtgacg catccggtga cgatgccgct ggtggacaat caactggatc tcgccgcttt
     6961 ctggcagcag tggctgaact ggcaggcacc ggttaatcaa cgcgcgtggg cgtttcatga
     7021 tgcgctggcg cagggttttg ccgcgttcat gcgtgagcag gccacgatgc gtggtatcac
     7081 tacgctggta tttagcggcg gggttattca taaccgtttg ctgcgtgcac gtctggcgca
     7141 ttatctcgct gatttcacat tgctctttcc acagagttta ccggcgggtg atggcggttt
     7201 gtctctgggg cagggggtta ttgctgcggc gcgttggtta gcgggtgaag tccagaacgg
     7261 ataaaacgtg ttgcatttgg acaccattgc cggatgcggc g
//
LOCUS       DAJSKJ010000177         7294 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000177 DAJSKJ010000000
VERSION     DAJSKJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.179"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            375..1412
                     /gene="dusA"
                     /locus_tag="ON345_003332"
     CDS             375..1412
                     /gene="dusA"
                     /locus_tag="ON345_003332"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR3073343.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1546..1788
                     /gene="pspG"
                     /locus_tag="ON345_003333"
     CDS             1546..1788
                     /gene="pspG"
                     /locus_tag="ON345_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR3073344.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1954..2937)
                     /locus_tag="ON345_003334"
     CDS             complement(1954..2937)
                     /locus_tag="ON345_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR3073345.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3020..4435
                     /gene="dnaB"
                     /locus_tag="ON345_003335"
     CDS             3020..4435
                     /gene="dnaB"
                     /locus_tag="ON345_003335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR3073346.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4488..5567
                     /gene="alr"
                     /locus_tag="ON345_003336"
     CDS             4488..5567
                     /gene="alr"
                     /locus_tag="ON345_003336"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR3073347.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRVTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5820..7013
                     /gene="tyrB"
                     /locus_tag="ON345_003337"
     CDS             5820..7013
                     /gene="tyrB"
                     /locus_tag="ON345_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR3073348.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1731 a         1777 c         1922 g         1864 t
ORIGIN      
        1 tcggagaaat aatatgtgaa gttcttcgat agtatggaag gcattatata aaaggaccca
       61 atatttattg ggttcttttt tctctatcaa tgctattagc agggagatat atcaccagag
      121 tttaatgtgt gattttttat ttatcgtcga acctggattg tttatcattg gccttaacaa
      181 agttaacggc taataagatt atttccatca cttcgtgaga gcttcatgcc ttgagaggat
      241 ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt tatggtatag taccccgcta
      301 ttgagcctcc tgaatagtga tgctgaataa cataacccca tgatatatcg ataaaataat
      361 ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa aatcaaccaa accagcgcaa
      421 tgcctgaaaa aactgacgtt cactggagtg gtcggtttag cgttgcacca atgctcgact
      481 ggacggacag acattgccgc tatttcttgc gtctgctttc ccgcaatacg ttgctgtata
      541 ccgaaatggt gaccacaggg gcgattattc acggtaaagg tgattacctg gcgtacagtg
      601 aagaagaaca tccggtagcg ttgcaactcg gcggtagcga tccggcggcg ctggcacagt
      661 gtgcgaagct ggcagaagcg cgtggatatg atgagatcaa cctgaatgtc ggctgcccgt
      721 ctgaccgggt gcagaacggc atgtttggtg cgtgtctgat gggtaatgcg cagctggttg
      781 ccgactgcgt gaaagcgatg cgcgatgtgg tgtcgattcc agtgacggtg aaaacgcgta
      841 ttggcatcga tgaccaggac agctatgaat ttctctgcga tttcatcaac accgtttccg
      901 gcaaaggcga gtgtgagatg tttatcatcc acgcacgtaa agcctggctt tcggggttaa
      961 gcccgaaaga aaaccgtgaa atcccgccgc tcgattatcc gcgtgtgtat caactgaagc
     1021 gtgactttcc gcatctgaca atgtcgatta acggtggtat caagtcgctg gaagaggcca
     1081 aagcacacct gcaacatatg gatggcgtga tggtcgggcg cgaggcgtat cagaatccgg
     1141 gtattctggc ggcggtagac cgggagatct ttggttcctc ggataccgat gccgatccgg
     1201 tggcggtagt gcgcgccatg tatccgtaca ttgagcgtga actcagccag gggacgtatc
     1261 tcggccatat tacccggcat atgttgggct tgttccaggg tattcctggc gcgcggcagt
     1321 ggcggcgtta tttaagtgaa aatgcccata aagcgggtgc agacattaat gtgctggaac
     1381 acgcgctcaa actggtggcg gataagcgtt aacttttcac caaaaagtag tcaaattcac
     1441 cacgccctgc gcaccgtcgc ggggcgtttt gctgttaaat caatagatta tttttggcat
     1501 gattcttgta atgccagcaa gagatttcat atttgggaga gcatcatgct ggaactactt
     1561 tttgtgattg gcttttttgt catgctgatg gtcaccggcg tttcgttgct gggcattatc
     1621 gccgcgctgg ttgtggcgac ggccattatg ttcctcggcg gtatgctggc attgatgatt
     1681 aagttgctgc cgtggttact actggcgatt gcggtggtgt gggttattaa ggcgattaaa
     1741 gcaccaaaag tgccgaaata tcagcgttat gaccgctggc gttactaagg gattgtgcgg
     1801 atgatcacaa cctaaggttt tatccttaga acaaaatagg aattgataat caaatctgtc
     1861 actattgcgc ctctaacaga ttcatcgtgc tgtaccctac atacagccga actataaaaa
     1921 gaaagggctt cccaggtgga agccctattt cttttatgga atcaacaggc tggaaccttg
     1981 cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca tccttcagcg gatatttctg
     2041 ctgctcggcg acatcgacct taatcacacc gctggcaatc aaagagaaca gttcattact
     2101 ggcctcggtt aattcctccc gcgtggtgat atagccttgc agggaagggc gtgtcacata
     2161 caacgagcct ttttgattga gaatgcctaa gttcacaccg gtaaccgcac ctgatgagtt
     2221 gccaaaactg accattaagc cgcggcgttg caggcaatcc agcgaccgtt cccaggtgtc
     2281 tctgcccacg gaatcgtaca ccacgcgcac tttcttaccg ccggtgatct cttttaaccg
     2341 ctcgaccaga tcctcttcac gatagttaat aacctgccac gcgcccgctt ttagcgcgct
     2401 ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc gcgcccaggg cttttgccca
     2461 ctggcaggca attaagccaa cgccgccagc cgctgcgtgg aacaggaact gttcatcggg
     2521 tttaatttca taggttttgc gcagcagata ataaaccgtt aagcctttca ggaaggatgc
     2581 cgcagcttgc tcaaaagaaa ttgccgcagg cagaatcgcc gctttatccg cattaatgtt
     2641 atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag actacacgat cgcctgcctt
     2701 aatatgcttt acaccactgc cgactttact cacgatgcct gccgcttcgg tacctaatcc
     2761 gctgggtagt gatggcggcg ggtaaaggcc gctgcggata tacgtgtcga taaaattgat
     2821 accgatggct ttattttcga cctggatttc attctctgcc ggatcggcag gagtgaactc
     2881 tacggcttga agtacttccg ggccaccgtg cttgtgaaat tcaattcgtg ttgccatgtg
     2941 tcctccagaa cgtatcgtca gggtctgctt catatggtaa agtttcgacc cattctttat
     3001 ctcggtaact ccattcacta tggcaggaaa taaacccttc aacaaacagc aggctgaacc
     3061 ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct ccgcactcga tcgaagcgga
     3121 gcagtcggtg ttgggcggtt taatgctgga taacgaacgc tgggacgatg tagccgagcg
     3181 tgtggtggca gacgattttt acacccgccc acaccgccat atctttactg aaatggcgcg
     3241 tttgcaggaa agcggtagtc ctatcgatct gattaccctt gcggaatcgc tggaacgcca
     3301 ggggcaactc gatagcgtcg gtggttttgc ttatctggca gagctgtcaa aaaatacgcc
     3361 aagtgcggct aacatcagtg cttatgctga catcgtgcgt gaacgtgccg ttgtccgtga
     3421 gatgatctcg gttgcgaatg agattgctga agccggtttt gatccgcagg ggcgcaccag
     3481 cgaagatctg ctggacctgg ctgaatcccg cgtctttaaa attgctgaaa gtcgtgcaaa
     3541 caaagacgaa gggccgaaga acatcgccga tgtgctcgac gcaaccgtgg cgcgtattga
     3601 gcagttgttt cagcagccac acgatggcgt taccggggta aacaccggtt atgacgatct
     3661 caacaaaaaa accgctggct tgcagccgtc ggatttgatc atcgtcgccg cgcgtccgtc
     3721 gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac gcggcgatgt tgcaagataa
     3781 accagtactt atcttctcgc tggagatgcc atcagaacag attatgatgc gttctctggc
     3841 gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg cagctcgatg atgaagactg
     3901 ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa cgcaatatct atatcgatga
     3961 ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca cgccgtattg cccgtgaaca
     4021 cggaggcatc gggcttatca tgatcgacta cctgcaactg atgcgcgtac cggcgctttc
     4081 cgataaccgt acgctggaaa ttgcagaaat ctcccgctcg ctgaaagcac tggcgaaaga
     4141 attgaacgtg ccggtggtgg cgctgtccca gttgaaccgt tctctggaac aacgtgccga
     4201 caaacgcccg gtcaactccg acctgcgtga atctggttct atcgagcagg atgcggactt
     4261 gatcatgttt atctatcgtg atgaggtgta tcacgaaaac agtgatttaa aaggcatcgc
     4321 ggaaattatt atcggtaaac aacgtaacgg cccaatcggg acggtacgcc tgacctttaa
     4381 cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag tacgacgacg aataataatt
     4441 attttatgaa ttaggtaatt aaagcaaaca cttatcaagg aacacaaatg caagcggcaa
     4501 ctgttgtgat taaccgccgc gctctgcgac acaacctgca acgtcttcgt gaactggcac
     4561 ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta tggtcacggt cttcttgaga
     4621 ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc ccgtctcgaa gaagctctgc
     4681 gactgcgtgc ggggggaatc accaaacctg tactgttact cgaaggcttt tttgatgcca
     4741 gagatctgcc gacgatttct gcgcaacatt ttcataccgc cgtgcataac gaagaacagc
     4801 tggctgcgct ggaagaggct agcctggacg agccggttac cgtctggatg aaactcgata
     4861 ccggtatgca ccgtctgggc gtaaggccgg aacaggctga ggcgttttat catcgcgtga
     4921 cccagtgcaa aaacgttcgt cagccggtga atatcgtcag ccattttgcg cgcgcggatg
     4981 aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt taataccttt tgcgaaggca
     5041 aacctggtca acgttccatt gccgcatcgg gtggcattct gctgtggcca cagtcgcatt
     5101 ttgactgggt gcgcccgggc atcattcttt atggcgtctc gccgctggaa gatcgctcca
     5161 ccggtgccga ttttggctgt cagccagtga tgtcactaac ctccagcctg attgccgtgc
     5221 gtgagcataa agccggagag cctgttggtt atggtggaac ctgggtaagc gaacgtgata
     5281 cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta tccgcgcgcc gcgccgtccg
     5341 gtacgccagt gctggtgaac ggtcgcgaag taccgattgt cgggcgcgtg gcgatggata
     5401 tgatctgcgt agacttaggt ccacaggcgc aggacaaagc cggggatccg gtcattttat
     5461 ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac gaaagtaagc gcttacgaac
     5521 ttattacgcg cctgacttca agggtcgcga tgaaatacgt ggattaatcg ttctgtaata
     5581 tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc ggccaataaa atcctaaaaa
     5641 ttcaataagt tgatgttctt tcatgctctt ataaaggtcg tgcctctggc ggatgtacgt
     5701 ttatcattag tctcactctg ttgctaattg ccgttcgctc ctgaacatcc actcgatctt
     5761 cgtcttcttc cggtttattg tgtcttaacc acctgcccgt aaacctggag aaccatcgcg
     5821 tgtttcaaaa agttgacgcc tacgctggcg acccgattct tacgcttatg gagcgtttta
     5881 aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg tctgtactac aacgaagacg
     5941 gaattattcc acaactgaaa gccgtggcgg aggcggaagc gcgcctgaat gcgcagcctc
     6001 atggcgcttc gctttattta ccgatggaag ggcttaacag ctatcgccat gccattgcgc
     6061 cgctgctgtt tggtgcggac catccggtac tgaaacaaca gcgcgtagca accattcaaa
     6121 cccttggcgg ctccggggca ttgaaagtgg gcgcggattt cctgaaacgc tacttcccgg
     6181 aatcaggcgt ctgggtcagc gatcctacct gggaaaacca cgtagcaata ttcgccgggg
     6241 ctggattcga agtgagtact tacccctggt atgacgaagc gactaacggc gtgcgcttta
     6301 atgacctgtt ggcgacgctg aaaacattac ctgcccgcag tattgtgttg ctgcatccat
     6361 gttgccacaa cccaacgggt gccgatctca ctaatgatca gtgggatgcg gtgattgaaa
     6421 ttctcaaagc ccgcgagctt attccattcc tcgatattgc ctatcaagga tttggtgccg
     6481 gtatggaaga ggatgcctac gctattcgcg ccattgccag cgctggatta cccgctctgg
     6541 tgagcaattc gttctcgaaa attttctccc tttacggcga gcgcgtcggc ggactttctg
     6601 ttatgtgtga agatgccgaa gccgctggcc gcgtactggg gcaattgaaa gcaacagttc
     6661 gccgcaacta ctccagcccg ccgaattttg gtgcgcaggt ggtggctgca gtgctgaatg
     6721 acgaggcatt gaaagccagc tggctggcgg aagtagaaga gatgcgtact cgcattctgg
     6781 caatgcgtca ggaactggtg aaggtattaa gcacagagat gccagaacgc aatttcgatt
     6841 atctgcttaa tcagcgcggc atgttcagtt ataccggttt aagtgccgct caggttgacc
     6901 gactacgtga agaatttggt gtctatctca tcgccagcgg tcgcatgtgt gtcgccgggt
     6961 taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc tgcggtgatg taatgcagga
     7021 aagcaggctg gagttaccca gcctgcagtg aaattaaact gtcgtcgctt tcactatttc
     7081 tttgtagatg atttttttga tgccgttgtt ctacgtgaga gataataaaa cgttgtcagt
     7141 tcttttattg ttaagtttat cccaattatc tggaattcct ttccctattt tttcgtgtgg
     7201 agtaataatt tctgcacatc taaccattat taaatttaat aataagtatc gggtttgtta
     7261 cgatagaata tttctatatc ctcaagaata tgct
//
LOCUS       DAJSKJ010000178         7286 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000178 DAJSKJ010000000
VERSION     DAJSKJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.180"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            224..1558
                     /gene="cadB"
                     /locus_tag="ON345_003338"
     CDS             224..1558
                     /gene="cadB"
                     /locus_tag="ON345_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR3073349.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     LLITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1638..3785
                     /gene="cadA"
                     /locus_tag="ON345_003339"
     CDS             1638..3785
                     /gene="cadA"
                     /locus_tag="ON345_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR3073350.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3844..5301
                     /gene="dtpC"
                     /locus_tag="ON345_003340"
     CDS             3844..5301
                     /gene="dtpC"
                     /locus_tag="ON345_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCR3073351.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            5538..7055
                     /gene="lysS"
                     /locus_tag="ON345_003341"
     CDS             5538..7055
                     /gene="lysS"
                     /locus_tag="ON345_003341"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3073352.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7174..>7286)
                     /gene="ghoT"
                     /locus_tag="ON345_003342"
     CDS             complement(7174..>7286)
                     /gene="ghoT"
                     /locus_tag="ON345_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCR3073353.1"
                     /translation="IWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT         1797 a         1698 c         1773 g         2018 t
ORIGIN      
        1 gtaaacattg aatgtttatc ttttcatgat atcaacttgc gatcctgatg tgttaataaa
       61 aaacctcaag ttctcactta cagaaacttt tgtgttattt cacctaatct ttaggattaa
      121 tccttttttc gtgagtattc ttagcgccag tttggtctgg tcaggaaata gttatacatc
      181 atgacccgga ctccaaattc aaaaatgata ttaggagaag agcatgagtt ctgccaagaa
      241 gatcgggcta tttgcctgta ccggtgttgt tgccggtaat atgatgggga gcggtattgc
      301 attattacct gcgaacctag caagtatcgg tggtattgct atctggggtt ggattatctc
      361 tattattggt gcaatgtcgc tggcgtatgt atatgcccga ctggcaacaa aaaacccgca
      421 acaaggtggc ccaattgctt atgccggaga aatttcccct gcatttggtt ttcagacagg
      481 tgttctttat taccatgcta actggattgg taacctggcg attggtatta ccgcggtatc
      541 ttatctttcc accttcttcc cagtattaaa tgatcctgtt ccggcgggta tcgcctgtat
      601 tgctatcgtc tgggtattta cctttgtaaa tatgctcggc ggtacttggg taagccgttt
      661 aaccactatt ggtctggtgc tggttcttat tcctgtggtg atgactgcta ttgttggctg
      721 gcattggttt gatgcggcaa cttatgcagc taactggaat actgcggata ccactgatgg
      781 tcatgcgatc attaaaagta ttctgctctg cctgtgggcc ttcgtgggtg ttgaatccgc
      841 agcagtaagt actggtatgg ttaaaaaccc gaaacgtacc gttccgctgg caaccatgct
      901 gggtactggt ttagcaggta ttgtttacat cgctgcgact caggtgcttt ccggtatgta
      961 tccatcttct gtaatggcag cttccggtgc tccgtttgca atcagtgctt caaccatcct
     1021 cggtaactgg gctgcgccgc tggtttctgc attcaccgcc tttgcgtgcc tgacttctct
     1081 gggctcctgg atgatgttgg taggccaggc aggtgtacgt gccgctaacg acggtaactt
     1141 cccgaaagtt tatggtgaag tcgacagcaa cggtattccg aaaaaaggtc tgctgctggc
     1201 tgcagtgaaa atgactgccc tgatgctcct catcactctg atgaactctg ccggtggtaa
     1261 agcatctgac ctgttcggtg aactgaccgg tatcgcagta ctgctgacta tgctgccgta
     1321 cttctactct tgcgttgacc tgattcgttt tgaaggcgtt aacatccgca actttgtcag
     1381 cctgatctgc tctgtactgg gttgcgtgtt ctgcttcatc gcgctgatgg gcgcaagctc
     1441 cttcgagctg gcaggtacct tcatcgtcag cctgattatc ctgatgttct acgctcgcaa
     1501 aatgcacgag cgccagagcc actcaatgga taaccacacc gcgtctaacg cacattaatt
     1561 aaaagtattt tccgaggctc ctcctttcat tttgtcccat gtgttgggag gggccttttt
     1621 tacctggaga tatgactatg aacgttattg caatattgaa tcacatgggg gtttatttta
     1681 aagaagaacc catccgtgaa cttcatcgcg cgcttgaacg tctgaacttc cagattgttt
     1741 acccgaacga ccgtgacgac ttattaaaac tgatcgaaaa caatgcgcgt ctgtgcggcg
     1801 ttatttttga ctgggataaa tataatctcg agctgtgcga agaaattagc aaaatgaacg
     1861 agaacctgcc gttgtacgcg ttcgctaata cgtattccac tctcgatgta agcctgaatg
     1921 acctgcgttt acagattagc ttctttgaat atgcgctggg tgctgctgaa gatattgcta
     1981 ataagatcaa gcagaccact gacgaatata tcaacactat tctgcctccg ctgactaaag
     2041 cactgtttaa atatgttcgt gaaggtaaat atactttctg tactcctggt cacatgggcg
     2101 gtactgcatt ccagaaaagc ccggtaggta gcctgttcta tgatttcttt ggtccgaata
     2161 ccatgaaatc tgatatttcc atttcagtat ctgaactggg ttctctgctg gatcacagtg
     2221 gtccacacaa agaagcagaa cagtatatcg ctcgcgtctt taacgcagac cgcagctaca
     2281 tggtgaccaa cggtacttcc actgcgaaca aaattgttgg catgtactct gctccggcag
     2341 gcagcaccat tctgattgac cgtaactgcc acaaatcgct gacccacctg atgatgatga
     2401 gcgatgttac gccaatctat ttccgcccga cccgtaacgc ttacggtatt cttggtggta
     2461 tcccacagag tgaattccag cacgctacca ttgctaagcg cgtgaaagaa acaccaaacg
     2521 caacctggcc ggtacatgct gtaattacca actctaccta tgatggtctg ctgtacaaca
     2581 ccgacttcat caagaaaaca ctggatgtga aatccatcca ctttgactcc gcgtgggtgc
     2641 cttacaccaa cttctcaccg atttacgaag gtaaatgcgg tatgagcggt ggccgtgtag
     2701 aagggaaagt gatttacgaa acccagtcca ctcacaaact gctggcggcg ttctctcagg
     2761 cttccatgat ccacgttaaa ggtgacgtaa acgaagaaac ctttaacgaa gcctacatga
     2821 tgcacaccac cacttctcct cactacggta tcgtggcgtc cactgaaacc gctgcggcga
     2881 tgatgaaagg caatgcaggt aaacgtctga tcaacggttc cattgaacgt gcgatcaaat
     2941 tccgtaaaga gatcaaacgt ctgagaacgg aatctgatgg ctggttcttt gatgtatggc
     3001 agccggatca tatcgatacg actgaatgct ggccgctgcg ttctgacagc acctggcacg
     3061 gcttcaaaaa catcgataac gagcacatgt atcttgaccc gatcaaagtc accctgctga
     3121 ctccggggat ggaaaaagac ggcaccatga gcgactttgg tattccggcc agcatcgtgg
     3181 cgaaatacct cgacgaacat ggcatcgttg ttgagaaaac cggtccgtat aacctgctgt
     3241 tcctgttcag catcggtatc gataagacca aagcactgag cctgctgcgt gctctgactg
     3301 acttcaaacg tgcgttcgac ctgaacctgc gtgtgaaaaa catgctgccg tctctgtatc
     3361 gtgaagatcc tgaattctat gaaaacatgc gtattcagga actggctcag aatatccaca
     3421 aactgattgt tcaccacaat ctgccggatc tgatgtatcg cgcatttgaa gtgctgccga
     3481 ctatggtaat gactccgtat gctgcgttcc agaaagagct gcacggtatg accgaagaag
     3541 tttacctcga cgaaatggta ggtcgtatta acgccaatat gatccttccg tatccgccgg
     3601 gagttcctct ggtaatgccg ggtgaaatga tcaccgaaga aagccgtccg gttctggagt
     3661 tcctgcagat gctgtgtgaa atcggcgctc actatccggg ctttgaaacc gatattcacg
     3721 gtgcataccg tcaggctgat ggccgctata ccgttaaggt attgaaagaa gaaagcaaaa
     3781 aataattagc tcgtacaagg gaagtggctt gccacttccc ttttttgctc ataaggagaa
     3841 cacatgaaaa caccctcaca gccgcgcgcg atatactata tcgtggcgat ccaaatctgg
     3901 gagtacttca gtttttacgg catgcgtgcc ttactcattc tctatctcac ccatcagctt
     3961 ggttttgatg ataaccatgc catcagcctg ttcagcgcat atgcttctct ggtttacgtt
     4021 acccctattc tcggcggctg gcttgccgac cgcctgctcg gcaaccgcac tgcagtgatt
     4081 gccggcgcgc tgttaatgac ccttggccat gtggtgctgg gcattgatac aaattcaacc
     4141 tttagcctgt atctggcgct ggcaatcatt atttgtggct acggtttatt caaatcaaac
     4201 atcagctgtt tgcttggcga gctctacgac gagaacgatc atcgacgtga tggcggtttt
     4261 tcgctgctgt atgctgcggg caatatcggt tctatcgccg ccccaatcgc ctgcggcctg
     4321 gctgctcagt ggtatggatg gcatgttggc tttgcccttg cgggtggcgg catgtttatc
     4381 ggtttgttga ttttcttaag cgggcatcgt catttccagt ccacgcgtag tatggataaa
     4441 aaagcgctca ctagcgtgaa atttgcctta ccagtatgga gctggttagt ggtgatgctc
     4501 tgtttagccc cagtattttt taccctgctg ctggagaacg actggtcggg atatttgctg
     4561 gcgatcgttt gcctcattgc cgcacaaatc attgcccgca tgatgatcaa attccccgaa
     4621 catcgccgtg ctctttggca aattgtattg ttgatgtttg tcgggacatt gttctgggta
     4681 ctggcacaac agggcggcag taccatcagc ttgtttatcg atcgctttgt gaatcgtcag
     4741 gcattcaata ttgaagtacc tacagcacta ttccagtcgg tgaatgcaat tgcggtgatg
     4801 ctcgctgggg ttgtactggc ctggctggcg tcgccagaaa gccgcggcaa ctcaacattg
     4861 cgcgtctggc tgaagtttgc ctttggctta ctgctgatgg cttgtggctt tatgttgttg
     4921 gcatttgatg cccgacatgc agcggctgac ggtcaagcgt caatgggcgt gatgatatcc
     4981 gggttggcgc taatgggctt tgccgaactc tttattgacc cggtggcgat tgcgcaaatc
     5041 acgcgtctga aaatgtctgg cgtattaacc gggatttata tgctggcaac aggcgcggtc
     5101 gccaactggc tggcaggcgt cgtggcacag cagacgacag agtcgcaaat tagcggtatg
     5161 gcaattgcag cttaccagcg attcttttct cagatgggag agtggacgtt ggcttgtgtc
     5221 gcgatcatcg tcgtattggc ctttgctacc cgttttctgt ttagcacgcc gacgaatatg
     5281 atacaggaga gcaacgatta actgtcgtaa aaacaattgg ttatgaacaa attaactttc
     5341 agtttatcac actagtaaat ctagattaat aagtcattta aaccattttg atggttattt
     5401 attagtgata tcaacttgag gtaagcgtta gtttcgataa gataaactga gttattaata
     5461 gtcgaggcag ataatacagt gtaccgatct gtctctttat ctacgctaaa ttgaaagctg
     5521 gatttagagg aaccaaaatg tctgaacaag aaacacgggg agccaatgag gctattgatt
     5581 ttaacgatga actgagaaat cgccgcgaaa aactggcggc actacgtcag caaggtgtgg
     5641 cgtttcccaa tgattttcgc cgcgaccata cctctgacca gttgcacgaa gagtttgatg
     5701 cgaagaataa ccaggaactg gaatccttaa acattgaagt ctcggttgct ggccgaatga
     5761 tgacccgtcg tatcatgggg aaagcctcct ttgtaacgtt gcaggatgtc ggtggccgta
     5821 ttcaactgta cgttgcaaga gatagcctgc cagaaggtgt ttataacgat cagtttaaaa
     5881 aatgggatct gggtgacatt atcggtgccc gcggtacgct gtttaagacg caaaccggtg
     5941 agctttccat tcactgtact gagttgcgcc tgctgacaaa agcactacgt cctttaccag
     6001 ataaattcca tggtctgcag gatcaggaag tccgttatcg tcaacgttat ctggacctca
     6061 tcgctaacga taaatcccgt caaacgttcg ttgtccgttc aaaaattctg gccgctatcc
     6121 gtcagttcat ggtcgcgcgc ggctttatgg aagtagaaac cccgatgatg caggtaattc
     6181 caggtggggc atctgctcgc ccgtttatta cccatcataa tgctctggat ttagatatgt
     6241 atctgcgtat cgcgccggag ctgtatctga aacgtctggt tgtaggcggt tttgaacggg
     6301 tattcgaaat caaccgtaac ttccgtaatg aaggtatttc tgttcgccat aatcctgagt
     6361 tcacaatgat ggaactctac atggcgtatg cggattacca cgatttgatt gaactgacag
     6421 agtccctgtt ccgcaccctg gcacaagagg ttctgggtac cactaaagtc acttatggcg
     6481 agcatgtgtt tgatttcggc aaaccgtttg aaaaactcac catgcgcgaa gcaatcaaaa
     6541 aatatcgtcc agaaaccgat atggccgacc tggataattt tgatgctgct aaagcattag
     6601 ctgaatctat cggaattacg gtagagaaaa gctgggggct gggacgtatt gtcacggaga
     6661 tctttgatga agtggcagaa gcacatctga ttcagccaac ctttattacg gaatatccgg
     6721 cagaagtgtc accgctggcg cgccgtaatg atgttaaccc ggaaatcacc gaccgttttg
     6781 aattcttcat cggtggtcgt gaaatcggta atggttttag cgaattaaac gacgcagaag
     6841 atcaggctga acgtttccag gaacaggtta acgctaaagc tgcaggtgac gacgaagcca
     6901 tgttctatga cgaagattac gtgactgcgc tggaatatgg tctgccgcca accgctggtc
     6961 tgggtattgg tatcgaccga atgattatgc tgtttacaaa cagtcatact attcgcgacg
     7021 ttattctctt cccggcgatg cgcccacaga aataaatttc actttaatga acgaagcagt
     7081 caggcgactg cttcgttcgt cgttccgcaa atccagtttt gaggagagac gcttcgtttc
     7141 acgataacca gcgggtccac tcaactacag ctcctaaaag agagaaaaaa gtaatgccac
     7201 aggcagactc attggccagg ttattccgac caggaatgca ctaagtaaac gaatatgtgt
     7261 tttctcatgt gagataaacc agataa
//
LOCUS       DAJSKJ010000179         7151 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000179 DAJSKJ010000000
VERSION     DAJSKJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.181"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            47..325
                     /gene="yafQ"
                     /locus_tag="ON345_003343"
     CDS             47..325
                     /gene="yafQ"
                     /locus_tag="ON345_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCR3073354.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            481..1221
                     /locus_tag="ON345_003344"
     CDS             481..1221
                     /locus_tag="ON345_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR3073355.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1192..1959)
                     /locus_tag="ON345_003345"
     CDS             complement(1192..1959)
                     /locus_tag="ON345_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR3073356.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     SCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKLLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2165..2743)
                     /gene="lpcA"
                     /locus_tag="ON345_003346"
     CDS             complement(2165..2743)
                     /gene="lpcA"
                     /locus_tag="ON345_003346"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR3073357.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2983..5427
                     /gene="fadE"
                     /locus_tag="ON345_003347"
     CDS             2983..5427
                     /gene="fadE"
                     /locus_tag="ON345_003347"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR3073358.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5470..5916)
                     /gene="ivy"
                     /locus_tag="ON345_003348"
     CDS             complement(5470..5916)
                     /gene="ivy"
                     /locus_tag="ON345_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR3073359.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6097..6864
                     /gene="yafV"
                     /locus_tag="ON345_003349"
     CDS             6097..6864
                     /gene="yafV"
                     /locus_tag="ON345_003349"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR3073360.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
BASE COUNT         1693 a         1794 c         1914 g         1750 t
ORIGIN      
        1 aggccaaaga cgccgatgat ttatttgata aattaggaat ttaaatatga ttcaaaggga
       61 tattgaatac tcgggacaat tttcaaagga tgtaaaactt gcacaaaagc gtcataagga
      121 tatgaataaa ttgaaatatc ttatgacgct tcttatcaat aatactttac cgcttccagc
      181 tgtttataaa gaccacccgc tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga
      241 accggactgg atcctgattt acaaacttac cgataaactt ttacgatttg agagaactgg
      301 aactcacgcg gcgctctttg ggtaaagccc gcttccgatg aaatttgtaa accctgcgcc
      361 gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc agactattcc
      421 gatttgggat ccattttgcc gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt
      481 atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt
      541 ttgctgggca gcagtagctc gaccacgcca gtgagcaaag agtataagca gcagttgatg
      601 ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa
      661 atgggcgagc aatatcagct gctcgacagt tataaaatct gtaaatattc cggcggatta
      721 gggccaaaac agcgtcaggg tgatttcaaa agcccggaag ggttttatag cgtccagcgt
      781 aatcagttaa aaccagacag ccgttactac aaagcgatta atattggttt ccccaatgcc
      841 tatgaccgtg cgcatggtta tgaagggaaa tatctgatga tccacggcga ttgtgtttcc
      901 atcggctgct acgcaatgac caatcagggt attgatgaga tcttccagtt cgttactggt
      961 gcgctggtgt ttggtcagcc gagcgtgcaa gtgagtattt acccgttccg catgaccgac
     1021 gccaatatga agcgccataa gtattccaac tttaaggact tctgggagca actgaaaccg
     1081 ggctacgact actttgagca aacccgtaag ccaccgaccg tttctgtggt caatggccgt
     1141 tacgtggtca gcaagccgtt aagccacgaa gttgtgcagc cacaactggc atcaaactac
     1201 acgctccccg aggcaaaata agcgccattc gcctggcata atcttttgcc aggtttcatt
     1261 gcccgtcagc ggctgtgttg caatcaccgt gaccacatca ttcggtgtgg tctgcgagct
     1321 gaagtcgatt tccacatcct gatccagcaa cgttgccacg ccaaacggcg cgcggcgggt
     1381 gatccagtgt aaattagtcg agcaatacgc cattacatag cgcccgtcag aaagcagcat
     1441 attgaaaacg cccttctgcc gcagttcatc cgccagtgag gcgatatatt taaacactgc
     1501 cgccatgttg cccggtgtac gcgggtaacg ctgcgttaat ttatgcagca gccagcagaa
     1561 ggctttttcg ctgtcggttt cgcctaccgg gcggaagttg ccggtttcca gtaatttgta
     1621 gcccgtcagt tgtccgttat gggcataagt ccagttacgc ccccataact cgcgggtaaa
     1681 tgggtgagta ttttccagcg ccacctcgcc ccgattagcc tggcgaatat gagccaccac
     1741 cgaacaggat tttatcgggt agtcctggac aagtttggcg atgggggaat taaagctggg
     1801 ttgtggatct ttaaatgtgc gacagctttt accttcgtaa aaggtaatgc cccagccatc
     1861 tttatgtggc ccggttccac caccacgctg tacaagtccg gtgaaactaa agcagatatc
     1921 ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg
     1981 gagcgcatgg cccccggcga agattgattt aagaattttc aggtcggatg cggcgtaaac
     2041 gaattattcg gcctacggtt cggatacaga tttgtaggca tgataagacg cgtcagcatc
     2101 gcatcaggca tcagcgcaca agtgccggat gcggcgtaaa cgccttatcc ggcctacgcc
     2161 agacttactt aaccatctct ttttcaatta actggatcag gatatggatc actttaatgt
     2221 gaatctcctg aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg atatccgccg
     2281 tgccagccat tttgccgccg tctttaccgg tcagggtgat cactttcatc cccttctcac
     2341 gcgccgctgc gatcgctttg atcacgtttg cagagttacc ggaggtggag atccccagca
     2401 gtacatcgcc ttcgcgacct accgcttcaa cgtagcggga gaaaatatca ttgaaaccga
     2461 aatcattacc gacgcaggaa atatgactaa cgtcagaaat agcaatcgcc gggtagcccg
     2521 gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg
     2581 aaccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctgtct gctaacagga
     2641 ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta gccagcgttt
     2701 ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata tccttcagca
     2761 taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa gtacgggcag
     2821 gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca atgtgagctt
     2881 tgttgtaatt atattgtaaa cacattgcta aatgttttta catccactac aaccatatca
     2941 tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat tttgagtatt
     3001 ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt atttatcagc
     3061 agtctgattt tgctcgcctg gacagccgcc ctcggcgttg ctggtctgtg gtcggcgtgg
     3121 gtactggtgc ctctggccat tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg
     3181 atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact
     3241 gagaaagaag cgattgatgc gggcaccacc tggtgggagg gcgacttgtt ccagggcaag
     3301 ccggactgga aaaagctgca taactatccg cagccgcgcc tgaccgccga agagcaagcg
     3361 tttctcgacg gcccggtaga agaagcctgc cggatggcga atgatttcca gatcacccat
     3421 gagctggcgg atctgccgcc ggagttgtgg gcgtacctta aagagcatcg tttcttcgcg
     3481 atgatcatca aaaaagagta cggcgggctg gagttctcgg cttatgccca gtctcgcgtg
     3541 ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt gccaaactca
     3601 ttaggcccgg gcgaactgtt gcaacattac ggcactgacg agcagaaaga tcactatctg
     3661 ccgcgtctgg cgcgtggtca ggagatcccc tgctttgcac tgaccagccc ggaagcgggt
     3721 tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggcgaatg gcagggccag
     3781 caggtgctgg ggatgcgtct gacctggaac aaacgctaca ttacgctggc accgattgcg
     3841 actgtgcttg ggctggcgtt taaactctcc gacccggaaa agctgctggg tggcgcagaa
     3901 gatttaggta ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt
     3961 cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa agatgtcttc
     4021 gtaccgatcg attacatcat cggtgggccg aaaatggccg ggcaaggctg gcggatgctg
     4081 gtggagtgcc tctcggtagg ccgcggcatc accctgcctt ccaactcaac cggcggcgtg
     4141 aaatcggtag cgctggcaac gggcgcgtat gctcacattc gccgtcagtt caaaatctct
     4201 atcggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg
     4261 atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa acctgccgtg
     4321 ctgtcggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat tattgatgcg
     4381 atggatatca ctggtggcaa aggcattatg ctcgggcaga gcaacttcct ggcgcgtgct
     4441 taccagggcg caccgattgc catcaccgtt gaaggggcca acattctgac ccgcagcatg
     4501 atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagca
     4561 gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc
     4621 ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg
     4681 ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc
     4741 gccctgcttt ctgatgtctc gatggctgtg ctgggcggca gcctgaaacg tcgtgagcgc
     4801 atctcggcac gtctgggtga tattttaagc cagctttacc tcgcctctgc cgttctgaag
     4861 cgttatgacg acgaaggccg taatgaagcc gacctgccgc tggtgcactg gggcgtacaa
     4921 gatgcgctgt atcaggctga acaggcgatg gatgatttac tgcaaaactt cccgaaccgc
     4981 gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcacct
     5041 tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc cacccgttcc
     5101 cgcattggtc gcggtcagta cctgacgccg agcgagcata atccggttgg cttgctggaa
     5161 gaggcgctgg tggatgtgat tgccgccgac ccaattcatc agcggatctg taaagagctg
     5221 ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg
     5281 ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt
     5341 attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag
     5401 aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt
     5461 atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc
     5521 cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca gccaggtgag
     5581 tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt
     5641 ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat
     5701 cacctgatac gtctcatctc ccaacgttac ggtttgtgcg ggagtataag taccgccttt
     5761 catcacccag gcaggcagct tatgcccttg taccatctga ttaaatgcag ctttggtggt
     5821 ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat
     5881 gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc
     5941 catgttatta accttctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc
     6001 aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttaa
     6061 agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt
     6121 ttgcagcaac cactggtgtg gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa
     6181 ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt
     6241 gccatggaag cggcagcttc gtcgctagca caagatgacg tagtgaactg gatgacagct
     6301 aaggcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac ggagtctggt
     6361 tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt
     6421 catctgttcc gcatggcaga tgagcatcta cattataaag cgggcaatgc gcgagtgatt
     6481 gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg ttttcctgtg
     6541 tggtcgcgca atctcaacga ttatgacctc gccctgtacg tcgccaactg gcctgctccg
     6601 cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg
     6661 gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg
     6721 gtgattaatc cacaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt
     6781 gatgccgagc tgtcgatggc ggtgttaagg gaatatcgcg ataagtttcc ggcatggcgg
     6841 gatgcggatg agtttaggtt gtagtgagtg ggggctgtat ttagggcggg gaattcagaa
     6901 agggatgttt agtgtgctga gcgagagtta aataataaaa aactgggaag atttaataat
     6961 ccccccgcag ggcaagagca tgaattaaat ttgtgttaag tttttcattt tgtcagttcg
     7021 aaacccaagc tctgagcagg atgcaagcac ttcttctata taacatgtgt ttaacgccag
     7081 ggcaagatta cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg
     7141 ctgcttttcg c
//
LOCUS       DAJSKJ010000180         7063 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000180 DAJSKJ010000000
VERSION     DAJSKJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.182"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            564..1553
                     /gene="araF"
                     /locus_tag="ON345_003350"
     CDS             564..1553
                     /gene="araF"
                     /locus_tag="ON345_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR3073361.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1623..3137
                     /gene="araG"
                     /locus_tag="ON345_003351"
     CDS             1623..3137
                     /gene="araG"
                     /locus_tag="ON345_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR3073362.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3152..4138
                     /gene="araH"
                     /locus_tag="ON345_003352"
     CDS             3152..4138
                     /gene="araH"
                     /locus_tag="ON345_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR3073363.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFVACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4305..5105
                     /gene="otsB"
                     /locus_tag="ON345_003353"
     CDS             4305..5105
                     /gene="otsB"
                     /locus_tag="ON345_003353"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR3073364.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLASASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5080..6504
                     /gene="otsA"
                     /locus_tag="ON345_003354"
     CDS             5080..6504
                     /gene="otsA"
                     /locus_tag="ON345_003354"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR3073365.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTHSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6511..6939)
                     /gene="uspC"
                     /locus_tag="ON345_003355"
     CDS             complement(6511..6939)
                     /gene="uspC"
                     /locus_tag="ON345_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR3073366.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1786 a         1640 c         1833 g         1804 t
ORIGIN      
        1 atgatagtgc tttttgtatc ttgttgatat taatcaatga atttgaattc atgatgaagt
       61 gatgatttta taaaacgttt tttcattttt gcgagatggc tctcattata cgtgttctgt
      121 taattaatga gcacagtgat aattaatggc gaaatgaatg acgtgcattt tccacatctt
      181 tgagttgcgg ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact
      241 ggttaaaaag aagacatccc gcatgggtac caaagacaac aaggatttcc aggctaatct
      301 tatggattaa tctgctgtgc attcgacaat ttgtctgaca aattggcttt cccttatgtc
      361 ttttcccgct aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct
      421 atggagaatt aatttctcgc taaaactatg tcaacacggt cacttatctt ttagataaaa
      481 ggtaatgctt tgttttccga ttaatttaac gaaagtcatt cgtttttgcc ctacacaaaa
      541 cgacactaaa gctggagaga accatgcaca aatttactaa agccctggca gccattggtc
      601 tggcagccgt tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga
      661 agcaaccgga agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg
      721 atttagggtt tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga
      781 tcgacagcct ggctgccagt ggcgcaaaag gtttcgttat ttgtactccg gaccccaaac
      841 tcggctctgc catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gccgtggatg
      901 accagtttgt taacgccaaa ggtaagccaa tggataccgt tccgctggtg atgatggcgg
      961 cgactaaaat tggcgaacgt cagggtcagg aactgtataa agagatgcag aaacgtggct
     1021 gggatgtcaa agaaagcgcg gtgatggcga ttaccgccaa cgaactggat acggcacgtc
     1081 gccgtacaac gggttctatg gatgcgctga aagcggccgg attcccggaa aaacaaattt
     1141 atcaggtacc taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc
     1201 tggttcaaca tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtgc
     1261 tgggcggcgt acgcgcgacg gaaggtcagg gctttaaagc tgccgatatc atcggcattg
     1321 gcattaacgg tgtggatgcg gtgagcgaac tgtctaaagc acaggcaacc ggcttctacg
     1381 gttccctgct gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact
     1441 gggtagcaaa agacgttgaa ccgccaaaat ttaccgaagt taccgacgtg gtgctgatca
     1501 cgcgtgacaa ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg
     1561 gaaaaattcc cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg
     1621 ttatgcaaca gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg
     1681 ttaaggcgct gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg
     1741 gtgaaaatgg cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa
     1801 ccacgggttc tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac
     1861 ttaacgcggg tgtggcgatt atttaccagg aactacatct cgtgccggaa atgaccgtcg
     1921 cggaaaacat ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc
     1981 tgaattatga ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc
     2041 cgctgaaata tctctccatt ggtcagtggc agatggttga aatcgccaag gcgctggcgc
     2101 gtaacgctaa aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg
     2161 acaatctttt ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt
     2221 ctcaccgtat ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac
     2281 gttatgtcaa aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga
     2341 tggtcgggcg cgacattggc gatatctacg gctggcaacc gcgtagttat ggcgaggagc
     2401 gcctgcgtct tgatgctgtg aaagcaccag gcgtgcgtac gccaataagt ctggcggttc
     2461 gcagtggtga aattgttggg ctgtttggtc tggtaggggc ggggcgtagc gaattaatga
     2521 aaggcatgtt tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga
     2581 tcgatattcg taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagatc
     2641 gcaaagcgga aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca
     2701 gacgtaaaca tgtgctcggc ggttgtgtaa tcaacaacgg ttgggaagaa aacaatgccg
     2761 atcaccacat tcgttcgctc aacatcaaaa cgccgggcgc ggagcaactg atcatgaatc
     2821 tctcaggcgg aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg
     2881 tcattttgct ggatgaacct acgcgcggca ttgatgttgg cgctaagcac gaaatatata
     2941 acgtaattta tgcgctggcg gcgcagggcg tggcggtgct gtttgcctcc agcgacttac
     3001 ctgaagtcct cggcgttgcc gaccggattg tggtgatgcg ggaaggtgaa atcgccggtg
     3061 aattgttaca cgagcaggca gatgagcgtc aggcattgag ccttgcgatg cctaaagtca
     3121 gccaggctgt tgcctgagta aggagagtat gatgtcttct gtttctacat cggggtctgg
     3181 cgcacctaag tcgtcattca gcttcgggcg tatctgggat cagtacggca tgctggtggt
     3241 gtttgcggtg ctttttgtcg cctgtgccat ttttgtcccg aactttgcca ccttcattaa
     3301 tatgaaaggg ttgggcctgg ctatttccat gtcggggatg gtggcttgtg gcatgttgtt
     3361 ctgccttgct tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt
     3421 caccacggcg gtggttatta acctgactga aagcctgtgg attggcgtgg cagcggggtt
     3481 gttgctgggc gttctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa
     3541 tgctctgatc acgacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc
     3601 agacggtaaa gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg
     3661 gttcggtctg cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgct
     3721 gctgaataaa accacctttg gtcgtaatac cctggcgatt ggcgggaacg aagaggctgc
     3781 gcgtctggcg ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct
     3841 ggtatcagcg atagccggaa ttattctggc ttcacgtatg accagtgggc agccaatgac
     3901 gtcgattggt tatgagctga ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa
     3961 aggcggcatc ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga
     4021 aaacgccatg aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat
     4081 cctgctggca gcggtgatct tcgaccgtta caagcaaaaa gcgaaacgca ctgtctgatg
     4141 cttttttcca caacaattta acgttttttc ccaccacagc cagccgccac aacggttggc
     4201 tgttcttcgt tgcaaatggc gacccccgtc acactgtcta tacttacatg tctgtaaagc
     4261 gcgttctgcg caacacaata agaaaagaga aggaggagaa ccgggtgaca gaaccgttaa
     4321 ccgaaacccc tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg
     4381 cggaaatcaa accgcatccc gatcaggtcg tcgtacctga caatattctg caaggacttc
     4441 agctactggc aagcgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg
     4501 agctggacgc actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc
     4561 gccgtgacat caatggtaaa acgcatatcg ttcatctgcc ggatgcgatt gcacgtgata
     4621 ttaacgtgca actgcatacg gtcatcgccc agtatcccgg tgcggagctg gagacgaaag
     4681 ggatggcttt tgcactgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat
     4741 tagcgcaacg tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg
     4801 tcgagatcaa accgagaggt accagtaaag gtgaggcgat tgctgccttt atgcaggaag
     4861 ctccctttat cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct
     4921 tcgcagtcgt taaccggctg ggcggaatgt cagtaaaaat tggcacaggc gcaactcagg
     4981 catcatggcg actggcgggc gtaccggatg tctggagctg gcttgaaatg ataaccaccg
     5041 cattacaaca aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta
     5101 tctaaccgga ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc
     5161 atactggggg cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg
     5221 aatgaggatc agccgctaaa aaaggtgaaa aagggtaaca ttacgtgggc ctcttttaac
     5281 ctcagcgaac aggaccttga cgaatactac aaccaattct ccaatgccgt tctctggcct
     5341 gcttttcatt atcgcctcga tctggtgcaa tttcagcgtc ctgcctggga cggctatcta
     5401 cgcgtaaatg cgttgctggc agataaatta ctgccgctgt tgcaagacga tgacattatc
     5461 tggatccacg attatcacct gttgccattt gcgcatgaat tacgcaaacg gggagtgaat
     5521 aatcgcattg gtttctttct gcatattcct ttcccgacac cggaaatctt caacgcgctg
     5581 ccgacatatg acaccttgct tgaacagctt tgtgattatg atttgctggg tttccagaca
     5641 gaaaacgatc gtctggcgtt cctggattgc ctttctaacc tgacccgcgt cacgacacat
     5701 agcgcaaaaa gccatacagc ctggggcaaa gcgtttcgaa cagaagttta cccgattggc
     5761 attgagccaa aagaaatagc caaacaggct gccgggccac tgccgccaaa actggcgcaa
     5821 cttaaagcgg aactgaaaaa cgtacaaaat atcttttctg tcgaacggct ggattattcc
     5881 aaaggtttgc cagagcgttt tctcgcctat gaagcgttgc tggaaaaata tccgcagcat
     5941 catggtaaaa ttcgttatac ccagattgca ccaacgtcgc gtggtgatgt gcaagcctat
     6001 caggatattc gtcatcagct cgaaaatgaa gctggacgaa ttaatggtaa atacgggcaa
     6061 ttaggctgga cgcctcttta ttatttgaat cagcattttg accgtaaatt actgatgaaa
     6121 atattccgct actctgacgt gggcttagtg acgccactgc gtgacgggat gaacctggtg
     6181 gcaaaagagt atgttgccgc tcaggaccca gccaatccgg gcgttcttgt tctttcgcaa
     6241 tttgcgggag cggcaaacga gttaacgtcc gcgttaattg ttaaccccta cgatcgtgac
     6301 gaagttgcag ctgcgctgga tcgtgcattg actatgtcgc tggcggaacg tatttcccgt
     6361 catgcagaaa tgctggacgt tatcgtgaaa aacgatatta accactggca ggagtgcttc
     6421 atcagcgacc taaagcagat agttccgcga agcgcggaaa gccagcagcg cgataaagtt
     6481 gctacctttc ctaagcttgc gtaggagcgg ttaatctccc gtaagtggaa ctaacagcac
     6541 atcgacttca cttgaggcaa tcactctttt cgccgagcag gatgctcgcg aaaagaaact
     6601 gtgattgtga ttaccgcaaa tcaccaaatc gaaatgatat ttgcgacaaa cttccagaat
     6661 atgttcgctt aactcaccgt aggcaataaa cgtcttgtca acgggatacc ccgcatcctg
     6721 aattaactta tcaagaaagc tttgcgtttc ttcctgcatc acgctgcgta aatcttcaag
     6781 catcggcgca gctaattgat tgtacatttc cgggtcggaa gcgagagtaa ttaaactgat
     6841 gtgtcccttt actggcctgg cgatagatac cgcttttgcc agcagttgct gactttccgg
     6901 tgtaaccgca acagcgacaa gaatattgct atagctcata acctgttcct tattctgtga
     6961 acttcaggtg acattaaagc tttactccta atggagaaac gacgcaatcc caactcagtc
     7021 aaacaggtaa atattagtct gattattagt aaagacaatt aat
//
LOCUS       DAJSKJ010000181         7061 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000181 DAJSKJ010000000
VERSION     DAJSKJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.183"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1554
                     /locus_tag="ON345_003356"
     CDS             <1..1554
                     /locus_tag="ON345_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073367.1"
                     /translation="KQVAYGFREASRQAYNLDVSAKNAGMRVDDFTRLSGAMRILGAD
                     SESANASIEGIFKAFNEAASGKNEGVMAAMAQIGAQIQKNSDGSVNTLKTLESIAKIF
                     PTLRPEQQKSAADALGLTPELLALMRDGERMKKLLAKSDEFGLTVDPALNQQLSEVNG
                     TMNELSASWDGLWQRSKNKALKTILSDGSVKDGLEGVTDLFTNGDFTGLSHALGFINS
                     NDAEKLRRIQNDKELYNSLPRSERGQVDAGFMTDAVRKRYDANYRATDSAIQLQNDLS
                     AISQPQSNVARGNVPYGETRNNAIGFRNNNPGNLRVAANATGKNGGFSTFANDADGRT
                     AMARQLMLYGDRGNNTLDGIIHTYAPQSENNTRAYIDSVSKATGYGAKEQVNLHDPET
                     LKTIMAAMIKHENGAQPYTEEQLLNAIQTAITDDRWSGKRNPDVLAQQRYDIISGSRG
                     VDQEPTILRTQQGKSDEVALSENLARSFKEAMSDQSLKLEITMVNDKGERKTYNAENN
                     GRITTAMNY"
     gene            1898..3301
                     /locus_tag="ON345_003357"
     CDS             1898..3301
                     /locus_tag="ON345_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR3073368.1"
                     /translation="MPSIIQDAITSLLGGDTSDDWQGQLRPSSFRGVPFAIVAEEGSH
                     GRRQAVHEYPYRDTAWIEDIGRATRRFVIRGFLIQNSQVYGGGDAITQRQSLIEACEQ
                     KGSGTLVHPTLGELTVSIPENGLRISGSMENGRVFEFTLMAIESGLKVFAVTGSTVAG
                     ATVKTNYLKLVSTAVLSTIARVKSEIRGVTQAINTIRGTVTFWTNMVDSTISQVTNLS
                     NVLNSTFGNTRYGRYSKGSVGGSSSAVAGKSSVADVDDERALADKVTAQSVMDRKNVT
                     DRSSQLSSSNTPDEFVQGVADVVNAILNSAGSVNDRITALEKLANSISTEYQQSDSSK
                     AISATMNTLIVVLCTGAMTSAAADSRPASTDEAEELTQRVSVQLDTALVLAGDHADDD
                     MYNALLAVRSAFLSTMSERASGLSELLQVTTAQPLPALTLANRLYQDATRADELVQEA
                     RVPHPAFMPTTMKVLRQ"
     gene            3298..4383
                     /locus_tag="ON345_003358"
     CDS             3298..4383
                     /locus_tag="ON345_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3073369.1"
                     /translation="MNADSDLDVVSLTVDGKIIEGWDSVRVTRGIERFPSDFDLGLMD
                     YFPGNEDRQLVEEGMSCEVRIGDDLTLTGYVDDWEPALSRSRHEVRATGRSKCQDLVD
                     CSAEWPNNVINASNALEIASRLASYYGITVTTDVDELVKVPQFTLNWGESPQEVIDRV
                     ARWSALLYYDQPDGNLLLTRVGTRRAASGIAEGVNVEQAYYRKSMADRFSDYVGVSMS
                     VSPIAGYSPDTAYDAVTLATARDPEAARMRYRKHISIVESTLMATQQAQSAIDWEMNR
                     RYGRSKQLSVTIDSWRDKDGKLWEPNTLIPVDLPTLRLPKTELLLAEVTYMRDDYGTH
                     ARMTLMPPEAFTVQPYAFYQQLAGFNT"
     gene            4380..4964
                     /locus_tag="ON345_003359"
     CDS             4380..4964
                     /locus_tag="ON345_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005174424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR3073370.1"
                     /translation="MNQNLKKAAVRIAGMLGIGRITSQKDSGEIQNVQYQTPLEVASS
                     PRMADFGFSSGLPAGSDVVIAFIGGDRSSPVVIASNHQGYRRTGLKSGETAIYNQWAM
                     EVLLTENGVFIDAKGKDVEVNNATNVTINASDGILAKAPFLKCTGDIIDNCESNTRTL
                     KELREAHNDHDHVVKGIQKGDDEATSEKTEEQVQ"
     gene            4961..5398
                     /locus_tag="ON345_003360"
     CDS             4961..5398
                     /locus_tag="ON345_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR3073371.1"
                     /translation="MSDFASFWNVDEMIADWQEGAGMLSTDHDLQTVILISLFTDRLA
                     RSDDDYGDSDRRGWWGDTGVDQQLGSRLWLLRREKLTTNVAIKAETYALEALKWLKDD
                     GVVSDVIPVAQIVMPNRLNLIIRYLSPGKDWQESRFYWIWEKL"
     gene            5400..6542
                     /locus_tag="ON345_003361"
     CDS             5400..6542
                     /locus_tag="ON345_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR3073372.1"
                     /translation="MPFKRKTLSELREENRNFLQAELENVGSLLRFANLKVVADMDAG
                     MAHLHYGYLDYMALQSNPFTATDESLAGWMALKRVYRKPASAAKSKDVKAVGAASRII
                     PAGTILNRGDGYQYAVDAEIKIQDSGEGHGGITAVLPDVTDDVTGGGANGNADAGTVL
                     TLDVNIAGVESQLTLIEAATGGADIEDEEAFRSRGLLSWQEPPQGGSDTDYKKWALEV
                     SGVTRAWVKRRLNGAGTVGVYIMCDGNLNDGFPVGTDGISQLEDWGAVKASGDQLSVA
                     DHIYPLQTDTAIVFVCSPIRKSIDFEIAGIKDANSTVVSNIESALTTLFFDEANPDGT
                     GKIDLSDINKSISNVDGTKGYILNSPSSNITFSVGEIPLLGEVDFV"
     gene            6539..>7061
                     /locus_tag="ON345_003362"
     CDS             6539..>7061
                     /locus_tag="ON345_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR3073373.1"
                     /translation="MSLYSQNDYASALGALLPRGRAWPRAPDTVQAAVLRALGSSYQR
                     SDNDADNLIKGAFPPTATSMLSEWESTLGLPDDCAIGEIGGISDRQRSVVSKLISNGG
                     LNRDYYIRVAAALGYTITITQFRPAMSGMSACGDALNGDEWPFTWRINAPETTIKYTL
                     AGSSYCGDPLASWG"
BASE COUNT         1869 a         1550 c         1929 g         1713 t
ORIGIN      
        1 aaacaggtcg cttatggatt tcgggaggca tcccgtcagg cctataatct tgatgtctcg
       61 gcaaaaaatg cgggaatgcg cgttgacgat tttacccgac tttccggggc aatgcgtatt
      121 cttggggcag acagcgagag cgctaatgca tcaatagaag gtattttcaa agcattcaat
      181 gaggctgcca gtggtaaaaa cgagggggtt atggcagcga tggcgcaaat tggtgctcaa
      241 atccaaaaaa acagcgatgg ttcagtaaat acccttaaaa cactggagtc tatcgcaaaa
      301 atttttccaa ccttgcgacc tgaacagcag aagtccgccg ctgatgcact tgggctgacg
      361 cccgaattgc tggcgctaat gcgtgacggt gagcgcatga aaaagctgct ggcgaaatcg
      421 gatgaatttg gtctgactgt ggatccggca ctaaatcagc aattgagtga agtgaacggc
      481 actatgaatg agctcagcgc atcctgggat ggtctgtggc aacgttcaaa aaacaaggca
      541 cttaagacca ttctttcgga tggttcagtc aaagacggcc ttgaaggtgt taccgatctg
      601 ttcactaatg gtgattttac tgggctgtct catgctctcg gttttatcaa cagcaatgat
      661 gctgagaaac tacggcgcat tcagaacgat aaggaacttt ataacagctt accccgcagt
      721 gaacgtgggc aggttgacgc gggtttcatg actgatgctg taagaaagcg gtacgatgcg
      781 aattaccgcg cgaccgattc tgcgattcaa ttgcagaatg acttatccgc tatcagccag
      841 ccacaatcca acgttgcacg cggcaatgtt ccttacgggg aaacaaggaa taacgcaatt
      901 ggcttcagaa ataataatcc cggtaatttg agggttgcag caaacgcaac gggtaaaaat
      961 ggcggatttt ctacctttgc gaatgatgcc gacggaagaa ctgcaatggc gagacagctg
     1021 atgttgtatg gtgacagggg gaataatact ctggatggga ttattcatac ctatgctccg
     1081 caatcagaga ataatactcg tgcatatatt gactccgtct caaaagccac tggatatgga
     1141 gccaaagagc aagtaaatct gcacgatccg gaaacattaa aaacgataat ggcagctatg
     1201 attaaacatg agaatggcgc acaaccttat actgaggaac aactgctaaa cgccatccag
     1261 accgccatta ctgatgatcg gtggtcaggg aagagaaatc cggatgtgct ggcccagcag
     1321 cggtatgaca ttatctctgg atcacgcggc gtggatcaag aacccacgat actcagaaca
     1381 caacaaggga aaagtgatga agttgccctt agtgaaaatc tggcgcggtc gtttaaggag
     1441 gccatgtccg atcagtctct caagcttgaa atcacgatgg tcaatgataa gggggagcgt
     1501 aaaacctata atgcggaaaa taatggcaga ataacaacgg ccatgaatta ctgatcactg
     1561 tcgtcatttc gttaaggaag aagttatgaa tgaaaaagtt tttggagcaa aagccattta
     1621 gactccaaag gtttttgcgc ttgtttacct ggtaattggc atttacctcg ttttttctgt
     1681 tgtctcaatg aatttcacgg cgattacgat atcggtggta agtgcattgc ttttacgtgt
     1741 gctttatgag ttcctaatga actcattcaa ggcgactgag catctttaca ggatcgccga
     1801 atctcttgac cgtaatggat ccagcgataa atagataagt catctcagtg catatgtaaa
     1861 ccgccgacat ggcggttttt ttatttccgg aggcgtgatg ccgtcaatta tccaggacgc
     1921 aataacttct cttttggggg gagataccag cgatgactgg caggggcagt tacggcccag
     1981 ctcattcaga ggtgtgccat ttgcaattgt tgctgaggaa gggagccacg gccgacgcca
     2041 ggcggtacat gaatatccct accgtgatac agcctggata gaggatatcg ggcgagcaac
     2101 acggcgattt gttattcgcg gtttcttgat ccagaacagc caggtttacg gcggcggcga
     2161 tgctatcacg cagcgccagt cactgattga agcctgtgaa caaaaaggta gcggtacgct
     2221 tgtccatccg acactgggcg aattaacggt ttccatccct gagaatggtt tgcgtatttc
     2281 cggttcgatg gagaacgggc gagtatttga atttaccctg atggcaattg aatcagggct
     2341 taaagtgttt gctgtcacgg gcagtaccgt tgcaggcgcc acggtgaaaa ccaactatct
     2401 gaaactggtc agcactgctg tgctgagcac gattgccaga gttaagagtg aaatccgcgg
     2461 tgtcacacag gctataaaca ccatcagagg cacggtcacg ttctggacta acatggttga
     2521 cagcaccatc agtcaggtca cgaatctcag caatgtcctg aactccacgt tcgggaatac
     2581 ccggtacgga cgttacagta aaggctctgt gggcggtagt tcctctgctg ttgctggcaa
     2641 atcgtcagtt gctgatgtgg atgatgagag agcactggct gacaaggtaa cagcccagtc
     2701 ggtaatggac cggaaaaatg ttaccgacag gtcgagccag cttagcagct ccaacacacc
     2761 tgatgagttt gtccagggcg tcgccgacgt ggtaaacgca attcttaaca gcgccggaag
     2821 cgttaatgac cgaatcacag cgctggaaaa actggctaat tcaatcagca cggagtacca
     2881 gcagtccgac agcagcaaag cgatttcggc gaccatgaac acgctgattg ttgtgctatg
     2941 tactggtgcc atgaccagtg ccgctgcgga ctccagacca gccagtacag acgaggcaga
     3001 agagttaact caacgagttt ctgtgcaact tgatacggcg ctggttctgg ctggagacca
     3061 cgcggacgat gatatgtata acgcgcttct cgccgtcaga tcggcattcc tttctacgat
     3121 gagtgagcgt gcttctggtc tgagcgagct tctgcaggtt actaccgctc agccgcttcc
     3181 ggcgctgacg ctggcaaacc gattatacca ggatgccacc cgtgcagatg aactggtaca
     3241 ggaagcgcgc gtaccgcatc cggcgtttat gccgacaacc atgaaggtac tgaggcaatg
     3301 aatgcagaca gcgatctgga tgttgtttct ttgacggtcg acggcaaaat catcgagggg
     3361 tgggattctg tccgggtaac gcggggtatt gagcgttttc cctctgattt cgatcttggg
     3421 ctaatggatt acttccctgg caacgaagat cgtcaactcg ttgaagaggg aatgtcttgt
     3481 gaagttcgta tcggagatga tctgacactg acgggatatg ttgatgactg ggaacccgca
     3541 ctatcgcgct cccgccatga ggtccgcgcc acgggcagga gcaaatgtca ggacctggtg
     3601 gattgctcag ccgagtggcc taacaacgtc atcaatgcca gtaatgcgct tgaaattgct
     3661 tctcgcctgg catcctacta cggcatcacc gtaaccacgg atgttgatga acttgtgaag
     3721 gtaccccagt tcactctgaa ctggggtgag tctccgcaag aagtcatcga tcgggtggcc
     3781 agatggtctg ctctgcttta ctacgatcag cccgatggaa acctgttact gacccgggtg
     3841 ggaacacgtc gtgcggcaag tgggatagcc gaaggggtaa atgtcgagca ggcatactac
     3901 cgcaaatcga tggctgacag gttttcagat tatgtcggtg tatcaatgag cgtttctcca
     3961 attgcagggt attcgcctga tacggcctat gacgctgtga ctctggcaac ggcgagagat
     4021 ccggaggccg cccgcatgcg gtaccgaaaa catatatcga ttgtggaaag taccctgatg
     4081 gctactcaac aggcacaaag tgcgatcgac tgggaaatga accggcggta cggacgttca
     4141 aaacagctct cggtaaccat cgattcctgg cgggataaag acgggaaact gtgggaacca
     4201 aacacattga tccccgttga tcttcccacc ttacggttgc cgaagactga attgctactg
     4261 gcagaagtca cctatatgcg cgatgactac ggcacccatg cacgcatgac gctgatgccg
     4321 cctgaagcat tcacggtgca gccgtatgcc ttctatcagc aattagcggg gtttaacaca
     4381 tgaaccagaa cttgaaaaaa gccgctgtgc ggattgctgg gatgctgggg attgggcgta
     4441 tcacatctca gaaagacagc ggggaaattc aaaacgttca ataccagacc ccactggagg
     4501 ttgccagttc gcctcggatg gctgatttcg gtttttcctc cgggcttcca gcaggttcag
     4561 atgttgttat tgcctttatt ggtggtgatc gctctagccc agttgtgatt gcatcaaacc
     4621 atcagggtta tagacgaacg gggctgaaat caggggaaac cgccatttat aatcagtggg
     4681 cgatggaagt gttgcttact gaaaacggcg tttttattga tgcgaaaggg aaagacgttg
     4741 aagtaaataa cgcaaccaac gtcaccataa acgccagcga tggaattctg gccaaggccc
     4801 cgtttttaaa gtgtaccgga gacattattg ataactgtga gagtaacact cgaacgctta
     4861 aagaactccg cgaagctcac aatgatcatg accatgtagt taaaggtatt cagaagggcg
     4921 acgacgaagc caccagtgag aaaacagagg agcaggttca atgagtgatt tcgcttcctt
     4981 ctggaacgtg gatgaaatga ttgccgactg gcaggaaggc gcggggatgt tatctaccga
     5041 tcatgacctc cagacggtga ttttgattag cctttttacc gatcgcctgg cacgttctga
     5101 tgatgattat ggggatagcg accgtcgtgg gtggtggggc gacacaggtg tagatcagca
     5161 gcttggttca cggctttggt tattgcgcag ggaaaaactg acgaccaatg tggcaataaa
     5221 agctgaaaca tacgcgcttg aagctcttaa atggctgaaa gatgatgggg ttgtaagtga
     5281 cgttatccca gtagcccaaa tcgttatgcc aaatcgcttg aaccttatta tccggtattt
     5341 atcgccgggg aaagactggc aggagagcag gttctactgg atatgggaga aactttaaaa
     5401 tgccgtttaa gcgaaagact ctgagtgaat tacgagaaga aaaccgtaat tttcttcagg
     5461 cggaactaga gaacgtaggc tctcttcttc gttttgccaa ccttaaggtt gttgctgata
     5521 tggatgcggg gatggcacac ctgcactatg gctaccttga ttatatggcg cttcagtcca
     5581 atcctttcac ggcaacggat gaaagtctgg ctggatggat ggccttaaaa cgcgtctata
     5641 ggaaacccgc cagcgcagct aaatcaaaag atgtgaaagc ggtcggggcc gcaagccgta
     5701 ttattccagc cggaacgatt ttgaacaggg gggacggata tcagtatgcg gttgatgcgg
     5761 agatcaaaat tcaagatagc ggggaagggc atggaggaat aaccgctgtt ttaccggatg
     5821 tcactgatga tgttactgga ggcggcgcga acggcaatgc cgatgcagga acggttctca
     5881 cgcttgatgt aaacattgcg ggagttgagt ctcaattaac gctaattgaa gcggctacag
     5941 gtggcgctga tatcgaagat gaagaggctt tccggagtcg tggcctgctt tcatggcaag
     6001 agccaccaca ggggggaagt gataccgatt ataaaaaatg ggctttggag gtctctggag
     6061 tcacacgagc ctgggtaaag cgcaggctta atggtgcagg cacggttggt gtttacatta
     6121 tgtgtgacgg taatcttaat gacggcttcc ctgttggaac tgatgggata tcacagcttg
     6181 aggactgggg ggcagtgaag gcatccggtg atcagctatc tgttgctgat catatttatc
     6241 cgctccagac cgatactgca atagtttttg tctgttcgcc tattaggaag agtattgatt
     6301 ttgaaatagc aggcataaag gatgctaaca gcacagttgt cagtaatatt gaaagcgctt
     6361 taacgactct attttttgat gaggctaatc cggatgggac cggtaaaatt gacctgtccg
     6421 atatcaataa aagcatcagt aatgtagacg gaacgaaggg atatattctt aatagtccgt
     6481 catcaaacat cacttttagt gttggtgaaa ttccgctgct tggtgaggtg gattttgtat
     6541 gagtctttat tcacaaaatg attatgcttc cgcacttggt gcgttactac cacggggccg
     6601 ggcatggcct cgtgcacctg acacggtgca ggcagcagta ttacgggctt taggaagctc
     6661 ataccagcgt tctgacaacg acgcggataa tcttattaaa ggagcttttc cgccgacagc
     6721 gacgagcatg ctttcagaat gggaaagtac gctgggattg cctgacgact gtgcaattgg
     6781 tgaaatcggt ggtatcagcg ataggcagcg ttctgttgta tctaagctga taagcaatgg
     6841 cggcttgaat agagactatt acattagggt tgctgcagcc ctcggttata ccattacgat
     6901 cacccagttt cgaccggcta tgagtggaat gtcagcatgt ggtgatgcgc tgaatggtga
     6961 tgaatggcct tttacctgga ggattaatgc accagagaca acaatcaaat acaccttggc
     7021 aggttcatcg tattgcggtg atccattagc ttcttggggt a
//
LOCUS       DAJSKJ010000182         7044 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000182 DAJSKJ010000000
VERSION     DAJSKJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.184"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            178..789
                     /gene="ruvA"
                     /locus_tag="ON345_003363"
     CDS             178..789
                     /gene="ruvA"
                     /locus_tag="ON345_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR3073374.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            798..1808
                     /gene="ruvB"
                     /locus_tag="ON345_003364"
     CDS             798..1808
                     /gene="ruvB"
                     /locus_tag="ON345_003364"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR3073375.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(1958..2743)
                     /gene="znuB"
                     /locus_tag="ON345_003365"
     CDS             complement(1958..2743)
                     /gene="znuB"
                     /locus_tag="ON345_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR3073376.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2740..3495)
                     /gene="znuC"
                     /locus_tag="ON345_003366"
     CDS             complement(2740..3495)
                     /gene="znuC"
                     /locus_tag="ON345_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR3073377.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPDTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3574..4506
                     /gene="znuA"
                     /locus_tag="ON345_003367"
     CDS             3574..4506
                     /gene="znuA"
                     /locus_tag="ON345_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR3073378.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTEKQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            4522..5844
                     /gene="mepM"
                     /locus_tag="ON345_003368"
     CDS             4522..5844
                     /gene="mepM"
                     /locus_tag="ON345_003368"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR3073379.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRHE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5964..6935
                     /gene="lpxM"
                     /locus_tag="ON345_003369"
                     /gene_synonym="msbB"
     CDS             5964..6935
                     /gene="lpxM"
                     /locus_tag="ON345_003369"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR3073380.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         1833 a         1741 c         1832 g         1638 t
ORIGIN      
        1 actgaatatg taatatttaa atatttgctt ccaatataac ctgtagaaaa aattctactg
       61 cgtcattttt cagaacatcg ggacgcctcg caagttttct tcatccttcg ctggatatct
      121 atccagcatt tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg
      181 ataggcagac tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg
      241 ggcggcgtag gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg
      301 ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtat
      361 ggttttaaca ataaacaaga gcgcacgttg ttcaaagagt tgatcaaaac caacggcgtc
      421 ggcccgaagt tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc
      481 gttgagcgtg aagaagtggg ggcgctggtg aaactgccgg gtattggcaa aaaaaccgcc
      541 gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg
      601 ccagccgccg acctggtact cacatcacct gccagcccgg cgaccgacga tgctgaacag
      661 gaagcggttg ccgcgctggt ggcgctgggc tataaaccac aagaagcaag ccgcatggtg
      721 agcaaaatcg ctcgccctga cgccagcagt gaaacattaa ttcgcgaagc cctacgtgcc
      781 gcgttatgag gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc
      841 cggaagatgt ggcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc
      901 cgcaggttcg ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc
      961 tcgatcatct gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg
     1021 tcgccaatga aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg
     1081 gcgatctggc agcgatgctc actaaccttg aaccgcatga cgtgttgttt attgatgaga
     1141 tccaccgtct ttcgccagtg gtggaagagg tgttgtatcc ggcaatggaa gactaccaac
     1201 tggatatcat gattggtgaa ggtccggcgg cacgttccat taaaattgat ttgccgccgt
     1261 ttaccctgat tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgatcgtt
     1321 ttggtattgt gcagcgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc
     1381 gcagcgcacg ctttatgggg cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc
     1441 gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg tcgagtgcgt gacttcgccg
     1501 aagtgaagca cgatggcacc atctcggctg atatcgctgc tcaggcgctg gatatgctga
     1561 atgtcgatgc cgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata
     1621 agttctttgg tgggccggta ggtctggata acctggcggc tgccattggc gaagaacgtg
     1681 aaaccattga ggatgtgctg gaaccttatt tgattcagca aggctttttg cagcgtacac
     1741 cgcgcgggcg tatggcgaca gtgcgggcgt ggaaccactt cggcatcacg ccgccagaaa
     1801 tgccgtaagt cggatcggtc gctcgcggtg catccgacac tggcagtgtt gcctgatgcg
     1861 ccgctattcg cgtcttatca ggcatatacc agagatagcg aaccataggt cggataaggc
     1921 gctcgcgccg catccgacaa atgtgttcag cgatagatta gctggcctgc tttttcatca
     1981 tactgataat aaataacagt gccgcacata gcaccaccga cggacccgcc ggcgtatcgt
     2041 aaaatgcgga aaaggttaaa ccgccagtta ctgccaccat tcccaccaaa acagcgacac
     2101 cggccatctg ttccggcgtg cgggcaaagc gacgcgctgt agctgcagga ataatcagca
     2161 gcgaagtaat aatcaacgcg ccgacaaatt tcatcgctac accaatcgtc aatgccgtca
     2221 ccagcatcaa caacaatttc acgcgctgta atttcacacc atcaacaaac gccagatccg
     2281 ggctaatcgt catcgacagc aaattgcgcc attgccagaa caaaatcgcc accacgatga
     2341 ccacgccaat tgcaatagag atgagatctt ctggcgtcac tgccagcaaa tcaccgaaca
     2401 ggtaagccat caaatcaaca cgaatattag acatcagact aacgaccacc aggcccagcg
     2461 acagggcact gtgcgccata atccctaata acgtgtcgat cgccagctgt ggacgcttct
     2521 ccagccatac cagaccgccc gccagcagca gcgtaacggc aattaccgca tagaatggat
     2581 tcacgtccag caacaaacca aacgcgacgc caagtaatga ggcatgagcc agcgtatcac
     2641 cgaaataaga catacgacgc cagaccacaa acgaacccag cggacccgcg gcacaggcga
     2701 gcatgatccc ggctaaccaa ccgggaaata ataattcaat catgagcgat catttccccg
     2761 acgcaaaaca attcgtccct gtaaatcgtg acgatgatta tgatgatggc gatagatacc
     2821 cagttgttca gcaccacgag gaccaaacat agaaataaac tccggatgca gggaaacaac
     2881 ttccggtgta ccggaacaac aaatgtggtg attcaggcaa agcacttcat cggtttttgc
     2941 cattaccaga tgcagatcgt gagagaccat taaaacgcca caatccagtt cgcgacgcaa
     3001 ctggtcaata aggtcatata acgccacctg accattcaca tccacgccct gagtgggttc
     3061 atccagcact aataattgcg gtcgatttaa caatgctcgc gctaacaata cacgctgtgt
     3121 ttcgccaccc gagagctttt gcatcggtgc gttaatcaga tgcccggcct ggacacgttt
     3181 cagtgcaggc aaaatatctt ctttatgtgt gtcagggcgt aagcgtaaaa aacggtttac
     3241 ggtcagtggc aacgtggtgt cgagatacag cttctgcggt acatagccga tgcgcagttt
     3301 tccgttgcgc ttgataaccc cttcatcggg tgttaccagc ccgagcacta cccgtaccag
     3361 tgtcgactta cctgcgccat ttggcccaag taaagtcaaa atttttccag gtttaagttc
     3421 cagcgacaca tcagagagga cgcggcgttg gccaaaagaa accgagacat tttccaggga
     3481 aaccagactt gtcatgttaa ttttagtctt gcagtagtca tgaaatgtta taatatcaca
     3541 cttctcatat tcattacgat gattggtcgc attatgttac ataaaaaaac gcttcttttc
     3601 gcagcattat ccgccgctct ctggggcggt gcaacacagg ccgcagatgc tgccgttgtc
     3661 gcttcgctta aacccgttgg gttcatcgct tctgccattg ctgatggggt aacagaaacg
     3721 gaggttttac tacctgacgg cgcttcagaa catgattatt cactgcgtcc atcagatgta
     3781 aaacgcttac agaacgcgga cttagtcgtt tgggttggtc cggagatgga agcgttcatg
     3841 caaaaaccgg taagtaaatt acctgaagcg aaacaggtaa cgattgcgca gctagagaat
     3901 gtgaaaccgc tgctgatgaa aagtattcac ggcgatgatg atgatcacga ccacgcggaa
     3961 aaaagtgacg aagatcacca tcacggcgat ttcaacatgc atctttggct ttccccagag
     4021 atagcgcggg ctacagcggt tgcaatccat ggaaaattag tggaacttat gccgcaaagt
     4081 cgagccaaac ttgacgccaa cctgaaggat tttgaggcac aattagcctc aaccgaaaag
     4141 caggttggta acgagctcgc gccgctcaag gggaaaggtt atttcgtttt tcacgatgct
     4201 tacggctatt tcgaaaaaca gttcggactg acaccgcttg gtcattttac cgttaaccct
     4261 gagattcaac ctggcgcgca gcgtttacat gaaataagaa cacagttggt tgagcaaaaa
     4321 gcaacctgcg tttttgctga gccacagttc aggccagcgg tcgttgagag cgtcgcacga
     4381 gggacatccg ttcgtatggg aacgttggat cccctgggga cgaatatcaa actgggtaaa
     4441 acaagctatt cagaattcct gaatcaatta gccaaccagt atgcgagctg cctgaaagga
     4501 gattaatgag gaagtgatta cgtgcaacag atagcccgct ctgtcgccct ggcgtttaat
     4561 aatttaccgc gaccacaccg cgttatgttg gggtcgctca ccgttcttac tctggccgtc
     4621 gctgtctggc ggccctatgt ttatcaccgc gatgccacgc caattgtcaa aaccattgag
     4681 ctggaacaga acgaaattcg ttcgctctta cctgaagcca gtgagccgat tgatcaagct
     4741 gcacaagaag atgaagccat tccccaggat gaactggatg acaaaatcgc cggtgaagcg
     4801 ggcgtgcatg aatatgttgt ttccactggc gatacgctaa gcagcattct caatcagtat
     4861 ggtattgata tgggtgatat cacccaattg gctgctgccg acaaagaatt gcgtaacctg
     4921 aaaatcggtc aacaactctc ctggacatta accgcggacg gcgaactgca gcgcctcacc
     4981 tgggaagtgt ctcgtcatga aacccgaacc tatgaccgta ctgccgctaa cggttttaaa
     5041 atgaccagcg aaatgcagca aggagagtgg gtcaacaatc tgctgaaagg taccgtcggg
     5101 ggaagctttg ttgccagcgc cagaaacgcc ggtttaacca gcgccgaagt gagcgcagtg
     5161 attaaagcca tgcagtggca aatggatttc cgcaaactga aaaaaggcga tgaatttgcg
     5221 gtgttaatgt cacgagaaat gcttgatggt aaacgtgagc aaagccaact gctgggcgta
     5281 cgtttgcgtt cagaaggtaa agattattac gcaatccgcg ctgaagatgg caaattctac
     5341 gatcgtaacg gtactggtct ggcgaaagga ttcttgcgat tcccgacggc gaaacagttc
     5401 cgtatctcgt ctaactttaa cccacgtcgt actaatccgg tgaccggccg cgttgcgcca
     5461 cacagaggtg ttgatttcgc aatgccacaa ggtacgccag tgctttcagt gggtgacggt
     5521 gaagtggtgg ttgccaaacg cagtggcgca gcaggttatt atgtggctat tcgtcatggt
     5581 cgcagctaca ccacgcgtta tatgcacttg cgcaagattc tggtgaaacc gggacagaag
     5641 gtgaaacgtg gcgaccgtat cgcgctttcc ggtaataccg gacgttcaac cgggccgcat
     5701 ctgcactatg aagtatggat aaaccagcag gccgtaaacc cgctgacggc aaaactgccg
     5761 cgtaccgaag ggctgaccgg ctccgatcgt cgcgaattcc tggcgcaggc caaagagatt
     5821 gtgccgcagc tacggtttga ttaattaaca tccattcgca gccggtacgc agtcagtacc
     5881 ggcttttttt atttggtgcg gggcaagttg cgccgctaca ctatcaccag attgattttt
     5941 gccttatccg aaactggaaa agcatggaaa cgaaaaaaaa taatagcgaa tacattcctg
     6001 agtttgataa atcctttcgc cacccgcgct actggggagc atggctgggc gtagcagcga
     6061 tggcgggtat tgctttaacg ccgccaaagt ttcgcgaccc cattctggca cggctgggac
     6121 gttttgccgg acgcctggga aaaagctcac gccgtcgtgc gttaatcaat ttgtcgctct
     6181 gctttccaga acgtagtgaa gctgaacgcg aagcgattgt tgatgagatg tttgccaccg
     6241 cgccgcaagc gatggcaatg atggctgagt tggcaatacg cgggccggag aaaattcagc
     6301 cgcgcgttga ctggcaaggg ctggagatta tcgaagagat gcgacgtaat aacgagaaag
     6361 tgatctttct ggtgccgcac ggttgggccg tcgatatccc tgccatgctg atggcctcgc
     6421 aagggcagaa aatggcagcg atgttccata atcagggcaa cccggttttt gattatgtct
     6481 ggaacacggt gcgtcgtcgt tttggcggtc gtctgcatgc gagaaatgat ggtattaaac
     6541 cattcatcca gtcggtacgt caggggtact ggggatatta tttacccgat caggatcatg
     6601 gcccagagca cagcgaattt gttgatttct ttgccaccta taaagcgacg ttgcccgcga
     6661 ttggtcgttt gatgaaagtg tgccgtgcgc gcgttgtacc gctgtttccg atttatgatg
     6721 gcaagacgca tcgcctgacg attcaggtgc gcccaccgat ggatgatctg ttagaggcgg
     6781 atgaccatac gattgcgcgg cggatgaatg aagaagtcga gatttttgtt ggtccgcgac
     6841 cagaacaata cacctggata ttaaaattgc tgaaaactcg caaaccgggc gaaatccaac
     6901 cgtataagcg caaagatctt tatcccatca aataaaaaaa gcctctcgcg aggagaggct
     6961 tccgccgtgt gataagttca agtttgcttc agaagattcg aaatctgttg aactatcatt
     7021 gaactgtagg ccggatgagg cgtt
//
LOCUS       DAJSKJ010000183         6947 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000183 DAJSKJ010000000
VERSION     DAJSKJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.185"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(45..1181)
                     /locus_tag="ON345_003370"
     CDS             complement(45..1181)
                     /locus_tag="ON345_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR3073381.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKWQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1184..1546)
                     /locus_tag="ON345_003371"
     CDS             complement(1184..1546)
                     /locus_tag="ON345_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR3073382.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKTTDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2083..3996
                     /gene="mtlA"
                     /locus_tag="ON345_003372"
     CDS             2083..3996
                     /gene="mtlA"
                     /locus_tag="ON345_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR3073383.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4226..5374
                     /gene="mtlD"
                     /locus_tag="ON345_003373"
     CDS             4226..5374
                     /gene="mtlD"
                     /locus_tag="ON345_003373"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR3073384.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5374..5961
                     /gene="mtlR"
                     /locus_tag="ON345_003374"
     CDS             5374..5961
                     /gene="mtlR"
                     /locus_tag="ON345_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR3073385.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5973..6182)
                     /locus_tag="ON345_003375"
     CDS             complement(5973..6182)
                     /locus_tag="ON345_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073386.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6467..6829
                     /locus_tag="ON345_003376"
     CDS             6467..6829
                     /locus_tag="ON345_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR3073387.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1727 a         1767 c         1791 g         1662 t
ORIGIN      
        1 tagtccttac aaggagcaaa gttccataat ctcccccctc cccctcaatg atccaaataa
       61 agataatgca tccagctggt cattcttagc agcactttcc gcatcaccga aacatggctg
      121 aaatggtcgg agtaaaccgc cacctgggcg tagatgccgt cgggtaaggc atcgatatca
      181 tcgttagggt ccagttcaat ggttcccagc acaccatccg tgccgggcac gaccgttaat
      241 gattgcaata ccccctgcgc ctgataagaa ccgcctggca cgacaggtaa aatactagtc
      301 agtttgccgt gaaacacctg cccaggtagc gcgttaaaca ccacttccgc atcatcgccg
      361 ggtttcagac gtaacagcga gttttgccga aattgggcga caatttgcca tttttgctcg
      421 gggatgaaga ccatcaccgg acgcagcggc aaggcagctg cgtatgtacc tgggcggatc
      481 agtacctgag taacgtagcc attgctcggc gcgcgaatga cagtctgctc aaggttatat
      541 tttgcttcag taagttgcgc tcttaagctc acaatctgcg attgctcgcc gttaaccata
      601 ctgtcgagct ggctctggat ctgcgcctgc tccgccaccg agcctttcac cagcgcatcc
      661 tgcgcgagga aattttgccg cgcatcgtcg atgtcacgtt ccgagaacgg attcaccgcc
      721 gcctggctgc ctttcaagta acgttgataa tttttaaaca gacggtcgcg ctccgctgaa
      781 acctgggtgg tgttggcctg cgcttcagtg agctgcgcac gcagcgtctt tatattatgc
      841 gtcgccgtca tcaggtcagc ctgaagtctg tcaactcgcg cctggtaacg aaccgggtcg
      901 agcttaaaaa gcacctcgcc cttttgaata agctgattat tcttgtcagt gacttcagta
      961 acaattcccg tcacctgtgg cgtgataggg atcgctatca ctgccttttg cgcggtaaaa
     1021 gtgtaagggt ggttgtagtt catcaacaaa atcaaaccac tcaccagaaa cacgcctccc
     1081 agcgccgccg tcgccagcgt ccactgattt accggaatgc ggaagatttt aaagaccgcc
     1141 cacgccagcg ccacgtaagt taaaacaatc aatagatcca taattagatc tccgggaacg
     1201 tggaatagtc agtctttttc tcggcggcga gttgatgctc cagccgggaa attcggtcag
     1261 aaagtgaggc gatttccggg tcagtcgttt tctcctgcga cgcaacatgt gactgcatac
     1321 cccagccacg ctccggttga tagagcgtcg cccagatcca cagaaacggc cagataacat
     1381 gcagagtaaa caggctcacc cagcccgccg tatgaatagc gtcggcatgg gggtgattgc
     1441 gcttttttgc aatcagataa gggatgtcat gtatcgcgat gattccataa aaaatcacca
     1501 gaaagacgaa gatcagcact cccaacgcga aatagtttag aaacatatct gcctcggatt
     1561 cacgtttatc agtgttgttt ttgggctggc agccagaagg gagtcaggct gatattttga
     1621 caataatccg ggttcgcgat tctcgccata acaccaaaga ataattttta gaggtgatga
     1681 gttgcttagt tacataacga ttgtatgacg aaggcataac atgctgtaga tcacatcagg
     1741 tgaacgccgt aagaaaatat cttgtgattc agatcacaaa gattcaacaa accatcaaaa
     1801 caaaaatgtg acactactca catttaaatg ccatttttag cgaaaatcgc cgccttgttg
     1861 cttttttaca caagcgtttt gtgatgaacg tcacgtcaat tacctctcta ccccctatat
     1921 ttatgtgatt gatatcacac aaaaggccgt cgactggaca gttaaccgat tcagtgccag
     1981 atttcgcagt atctacaagg tccggctacc tctgccgcca cattaacaaa aaacctcggg
     2041 cttccagcct gcgcgacagc aaacataaga aggggtgttt ttatgtcatc cgatattaag
     2101 atcaaagtgc aaagctttgg tcgtttcctc agcaacatgg tgatgccaaa tatcggcgcg
     2161 tttatcgcgt ggggtatcat caccgcgtta tttattccaa cagggtggtt accgaacgag
     2221 acgctggcga agctggtcgg gccgatgatc acttatctcc tgccgctgct gatcggttat
     2281 accggtggta agctggtagg cggcgaacgt ggcggcgtag tcggtgccat caccaccatg
     2341 ggcgttatcg tcggcgcaga catgccgatg ttcctcggtt ctatgattgc aggtccgctg
     2401 ggcggctggt gcattaagca cttcgaccgc tgggtagacg gtaagatcaa atccggtttt
     2461 gagatgctgg tgaataactt ctccgcaggc atcatcggga tgatcctcgc tattctggca
     2521 ttcctcggca ttggcccgat tgttgaagcc ctgtccaaaa tgctggctgc gggcgttaac
     2581 ttcatggttg tccatgacat gctgccgctg gcgtctatct ttgttgaacc ggcgaaaatc
     2641 ctgttcctca acaacgccat taaccacggt atcttctcgc cgctgggtat tcagcagtcc
     2701 catgaactgg gtaaatcaat cttcttcctg attgaagcta acccaggtcc aggtatgggc
     2761 gtgctgctgg cgtacatgtt ctttggtcgt ggtagcgcta aacagtctgc gggcggtgcg
     2821 gcaatcatcc acttcctggg gggtatccac gaaatctact tcccgtatgt gctgatgaat
     2881 ccgcgtctga tcctcgcagt catcctcggc ggtatgactg gcgtgttcac gctgactatc
     2941 ctgggcggtg gtctggtttc tccggcatct ccgggttcta tccttgctgt actggcgatg
     3001 acaccaaaag gtgcttattt cgctaacatc gcgggtgtgt gtgcggcgat ggctgtctcc
     3061 ttcgttgtct ctgctatttt gctgaaaacc agcaaagtga aagaagaaga tgatattgaa
     3121 gcagcaactc gtcgtatgca ggacatgaaa gctgagtcta aaggcacatc tccgctgtct
     3181 gcgggcgatg tgactaacga cctgagccac gtacgtaaaa tcatcgttgc ctgtgacgcc
     3241 ggtatgggtt caagtgcgat gggcgcaggc gtgctgcgta agaaaattca ggatgcaggt
     3301 ctgtcacaga tttcggtcac taacagcgcg atcaacaacc tgccgccaga tgtggacctc
     3361 gtcatcactc accgtgacct gaccgaacgc gctatgcgcc aggttccgca ggcgcagcat
     3421 atttcgctga ccaacttcct cgacagcggc ctgtacacca gcctaaccga acgtctcgtt
     3481 gctgcccagc gccatactga aaacgaagtg aaagtgaaag acagtctgaa agacagcttt
     3541 gacgattcca gtgctaacct gttcaaactg ggcgcggaga acatcttcct cggtcgcaaa
     3601 gcagcaacca aagaagaagc gattcgtttt gctggcgagc agctggtgaa aggcggttac
     3661 gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa aactgacccc gacttatctg
     3721 ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga aagatcgcgt actgaaaacg
     3781 ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg gtgaagaaga agatgacatt
     3841 gcccgtctgg tgattggtat tgcagcccgt aataacgagc acattcaggt tatcaccagc
     3901 ctgaccaatg cactggatga tgagtccgtc atcgagcgtc tggcacacac caccagcgtg
     3961 gatgaagtgc tggaactgtt ggcaggtcgt aagtaatcca atcccaccct ctccacatgg
     4021 agaaggtggg gttaattgcc tgatgcgcta cgcttatcag gcctacagga tgcatcacga
     4081 tttgttgaat ttgcacgttc ttgtaggccg gataaggcgc ttacgccgca tccggcgctg
     4141 cccctctcct cacggagagg gtttgggtga gggaaaagcc tcaccccagc cctctcgggt
     4201 aaaaacattg atgaaggtta atactatgaa agcattacat tttggcgcag gtaatatcgg
     4261 tcgtggcttt atcggtaaac tgctggcaga cgcgggtatc caactgacgt ttgccgatgt
     4321 caatcaggtg gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc atgtggttgg
     4381 tgaaaccgag caggtagata ccgtttccgg cgtcaatgct gtcagcagca ttggtgatga
     4441 tgtcgttgat ctgattgctc aggtcgattt agtcactacc gccgttggcc cggttgtgct
     4501 ggaacgtatt gctccggcta tcgccaaagg gctggtgaaa cgcaaagaac aaggtaatga
     4561 atccccgctg aacatcatcg cctgtgaaaa catggtacgc ggtaccacgc agctgaaagg
     4621 ccatgtgatg aacgccctgc cggaagacgc caaagcgtgg gtagaagaac acgttggctt
     4681 tgtcgattcc gccgttgacc gcatcgtacc gccttcggct tcggcaacta acgatccgct
     4741 ggaagtgacg gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc
     4801 actgccgaac atcccaggca tggagttaac cgacaacctg atggcatttg tcgaacgtaa
     4861 actcttcacc ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac tggctggtca
     4921 tcagaccatt cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat
     4981 ggaagaaagt ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca aacatgcggc
     5041 gtacatccag aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg atgtagagcg
     5101 cgtaggccgt cagccgctgc gtaaactgag tgctggcgac cgtctgatca agccactgct
     5161 cggtacgctg gaatacagcc ttccgcacaa gaatctgatt caggggattg ctggtgcaat
     5221 gcacttccgc agtgaagatg atccgcaggc tcaggaactg gcagcactga tcgctgacaa
     5281 aggtccgcag gcggcgctgg cacagatttc cggtcttgat gccaacagcg aggttgtatc
     5341 cgaggcggta accgcttata aagcaatgca ataatggtgg accaggcgca ggacaccctg
     5401 cgcccgaata acagattgtc agatatgcag gcaacaatgg aacaaaccca ggcctttgaa
     5461 aaccgtgtgc ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc
     5521 gtcgagcttt tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg caaagacgat
     5581 tacgcggtga agtatgctgt agaaccgtta ctcgacggcg acggtccgct gggcgatctt
     5641 tctgtgcgtt taaaactcat ttacgggttg ggcgtcatta accgccagga atacgaagat
     5701 gcggagctgc tgatggcatt gcgtgaagaa ctaaatcacg acggcaacga gtacgccttt
     5761 accgacgacg agatccttgg accctttggt gaactgcact gcgtggcggc gttaccaccg
     5821 ccgccacagt ttgaaccagc agactccagt ttgtatgcaa tgcaaattca gcgctatcaa
     5881 caggctgtgc gatcaacaat ggtcctttca ctgactgagc tgatttccag aatcagctta
     5941 aaaaaagcct ttcaaaagta agcaacgtct gcttactgcc cctctacctg cttcggccga
     6001 taaagccgac gataatactc cagacgttgt agatataacg gcacgctttc atcgggtatt
     6061 ccagacggaa tcgcgttacg gggaggaagt tttttcagat actcccggaa cgcctggctt
     6121 gatgccatga aatctacggc tttatcgata agaagcggaa cgttttcacc taatttgccc
     6181 atgatgatat ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg
     6241 ttcccggtaa agatgacatt aaggaaaacg tgctgaatcc tcaataatca tccgctgctg
     6301 tttattatct tttttctata gatttcttaa ttaatcaacg aattgcgtag tttttgagca
     6361 tgttaatgat tacgttgcag aactattaca atgtcatcag ttgtgaacaa agcacctggt
     6421 cgcgcatact aggggctata aatttatctt tatcagaagc catcacatga aagaagtcga
     6481 aaaaaacgaa atcaaacgtc tcagcgatcg cctggacgcc atccgccacc agcaggccga
     6541 tctgtcgctg gttgaagccg cagacaaata tgccgagctg gaaaaagaga aagccacgct
     6601 ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa aaactgagta aagaagcaca
     6661 gaagctgatg aagatgccgt ttcagcgcgc aattaccaaa aaagagcagg ctgatatggg
     6721 caagctgaag aaaagtgttc gcggactggt ggttgtgcac ccaatgaccg cgctgggccg
     6781 tgaaatgggc ctgcaggaga tgactggatt ctctaagact gcgttttaag cgcctcagac
     6841 gactggttcg atcatatcaa cccgcatctg gcttgcctta gctggtcaat gcggggactc
     6901 tcctgtctac tcgcctaatt accaaacaat tcactccctt tacgtcg
//
LOCUS       DAJSKJ010000184         6827 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000184 DAJSKJ010000000
VERSION     DAJSKJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.186"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..3751
                     /locus_tag="ON345_003377"
                     /pseudo
     CDS             1..3751
                     /locus_tag="ON345_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            3973..4239
                     /gene="hipB"
                     /locus_tag="ON345_003378"
     CDS             3973..4239
                     /gene="hipB"
                     /locus_tag="ON345_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCR3073388.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW"
     gene            4239..5561
                     /gene="hipA"
                     /locus_tag="ON345_003379"
     CDS             4239..5561
                     /gene="hipA"
                     /locus_tag="ON345_003379"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCR3073389.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMTFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK"
     gene            complement(5843..6790)
                     /locus_tag="ON345_003380"
     CDS             complement(5843..6790)
                     /locus_tag="ON345_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073390.1"
                     /translation="MPRYKKGIRNNCFHQNYTHDVLFPGATFRTRHNGECAILGRSDD
                     KSRRGYYVVEFKDSGIIKEAYGSHIKTGSVSDEAFPSSEEERRKLLMTPKYYGVGYIG
                     NGCHSTIENTRTHQRTRAFMLWHNMLARCYMTTKGKQYFKGYKGVTVCERWHNFQNFC
                     NDLPKLHGYNKWKDNPGEYELDKDYSHRRIYSADTVAFISTEENAREAGLRRVAMKIP
                     SGHYHEINKIRDEILMEAEDELKNNQIHYEVVLDGNMKVILSETPYGTVLFWPLTKKI
                     QRNCYMIDGDVQVYVHYLRWLILQWENRNPDINCVATTC"
BASE COUNT         1853 a         1500 c         1818 g         1656 t
ORIGIN      
        1 tcggtggcaa tgccaccgat gtcggaaccc tgaatgtgca ggatcaagac tctgtcatca
       61 cggtacgcag actctataat ggatatttcg gtaatggcgc agtcaatatt tccaataatg
      121 gactaattaa taacaaagaa tattcattgg tgggcgttga agacggttcc cacggtgtcg
      181 tcaacgtgac cgataaaggg cattggagtt tcctcggaac aggcgaagct ttccgctata
      241 tctatatcgg tgatgctggt gacggtgaac ttaatgtctc gcgttaaggt aaagtagatt
      301 cgggaattat cactgcgggg atgaaagaaa caggcacagg caacattact gttaaggata
      361 agaactccgt tatcactaat ctcggaacta atcttggtta tgacggccac ggcgaaatga
      421 atatcagtaa tgaggggctt gttgtcagca acggaggaag ctcactcggt tatggagaaa
      481 acggcgtcgg gaatgtcagc atcaccacgg gggggatgtg ggaggtcaat aagaatgtct
      541 ataccaccat tggtgttgcg ggcgtcggaa acctcaatat tagcgatggc ggtaagtttg
      601 tatcgcaaaa tattactttt ttgggcgata aaatgcgctc aaaggcgatg gtttgatgcg
      661 agtgcagctg tcatcctccg acaagatgtt tggctttacc catgcaacag ggactgaatt
      721 cgccggtgtt gcccaactga aagacagtac cttcactctg gaacgcgaca acaccgctgc
      781 gcttactcac gcgatgttgc agtctgacag tgaaaatacc acatcggtaa acgtgggaga
      841 gcaatccatt ggtggactgg ccatgaatgg cggtacgctc attttcgata cggatattcc
      901 tgctgcgacg cttgcagagg gatatatcag cgtcgatacg ctggttgtcg gcgcgggtga
      961 ctacacctgg aaaggccgta actatcaggt aaacgggacg ggcgacgtgc ttatcgacgt
     1021 gcctaaaccg tggaatgatc ccatagcgaa taaccctctg acgacgctca atttgctgga
     1081 acacgatgat agccatgtcg gcgttcaact ggtgaaggcg caaacggtta ttgggtcggg
     1141 tggctcatta acgttacgtg atttacaggg cgacgaggtg gaagcggaca aaacgttaca
     1201 cattgcgcaa aacggaacgg tggtcgccga gggtgattat ggattccgcc tcacgaccgc
     1261 accaggtgat ggtttgtacg ttaactatgg gctgaaagcg ctgaacatcc atggagggca
     1321 aaagctgacg ttagccgaac atggcggagc ctatggcgca acggccgata tgtcggcaaa
     1381 aatcggtggt gaaggggatc tggcaatcaa tacggtgcga caggtttctc tttccaacgg
     1441 tcagaacgac tatcagggga caacctacgt tcagatgggg acattacgta ccgatgcgga
     1501 tggcgcgctg ggcaacaccc gggaactgaa catcagcaac gcggccatcg tcgatcttaa
     1561 tggatcgacg cagacggtag agccattcac cgggcagatg ggttcgactg ttttgttcaa
     1621 agagggggcg ctgacggtaa ataaaggtgg gatcagtcag ggtgaactga caggtggcgg
     1681 aaacctgaat gttacagggg gaacgctggc tatcgagggg cttaatgcac gctacaatgc
     1741 gttaaccagc attagcccaa atgcggaagt cagcctcgat aatacgcagg ggttaggcag
     1801 aggaaatatt gccaataacg gtctgttaac gctaaaaaac gtgactggcg aactgcgtaa
     1861 tagcataagc gggaagggta tcgtgagcgc aaccgccagg acagatgtag agctggatgg
     1921 cgataatagc cgctttgtgg ggcaattcaa cattgataca ggcagcgcgc tcagcgtcaa
     1981 cgagcagaaa aacctgggtg atgcttccgt tatcaataat ggcctgctca ccatctccac
     2041 tgagcgtagc tgggcgatga cgcacagtat cagcggtagc ggtgatgtga caaaactggg
     2101 taccgggatc ctgactctta acaacgattc cgcggcgtat cagggtacga cggatatcgt
     2161 ggggggggaa attgctttcg gttccgactc tgccattaat atggcaagtc aacacattaa
     2221 tatccataac agcggtgtga tgtcgggaaa tgtcaccact gcaggtgatg tgaacgttat
     2281 gccagggggg acactgcgtg tcgctaaaac cacaatcggc ggcaaccagg agaatggcgg
     2341 cacggttcaa atgaacagtg aaggaggaaa accggggaat gtactgaccg ttaacggcaa
     2401 ctataccgga aacaatggcc tgataacgtt caacgcgacg ctgggcggcg ataattcgcc
     2461 caccgataag atgaacgtga aaagcgatac ccaagggaac actcgcgttc gggttgataa
     2521 cattggcggc gtcggtgcgc aaacggtcaa cggtattgaa ctcattgagg ttggcggtaa
     2581 ttctgcaggt aacttcgcgc tgaccaccgg aactgtcgaa gctggggcat acgtctacac
     2641 gctggctaaa gggaagggga atgacgagaa aaactggtat ctgaccagta aatgggacgg
     2701 cgttacgcca gcggatacac ccgatcccat caataatccc cctgttgtgg atccggaagg
     2761 cccatcagtt tatcgcccgg aggccggaag ctatatcagc aacattgccg cagccaactc
     2821 gctgtttagc catcgcttac acgaccgtct gggtgagccg cagtatacag attcactgca
     2881 ttctcagggg tcggcaagca gtatgtggat gcgtcatgtc ggagggcacg aacgttcaag
     2941 ggccggtgac ggtcagctaa atactcaggc taaccgctat gtattgcagc taggcggcga
     3001 tttggcgcag tggagtagca acgcgcagga tcgctggcat ctcggcgtca tggcaggcta
     3061 tgccaatcag cacagtaata ctcagagtaa tcgtgtgggt tataaatcgg atgggcgcat
     3121 cagtgggtac agcgctggga tttacgcgac ctggtatcag aacgatgcga ataagaccgg
     3181 cgcttatgtt gacagctggg cgctgtataa ctggtttgat aacagcgtca gttccgataa
     3241 ccgttctgct gacgactatg attctcgcgg tgtgacggcc tctgttgagg gtgggtatac
     3301 ctttgaagcg ggaacattta gcggcagcga agggacgctg aatacctggt acgtccagcc
     3361 acaggcgcaa atcacctgga tgggtgtgaa agattccgac catacccgga aagacggaac
     3421 gcgcattgaa acggaaggcg acggaaatgt gcaaacgcga cttggggtga aaacctacct
     3481 gaacagccat caccagcgtg acgatggtaa acagcgtgag ttccagcctt acattgaagc
     3541 gaactggatc aacaatagca aagtctacgc cgtgaagatg aatggtcaaa ccgtaggccg
     3601 tgaaggtgcg cgtaatctcg gtgaagtacg taccggggtt gaggcgaaag taaataacaa
     3661 ccttagcctg tgggggaatg tcggtgtgca actaggtgat aaaggctata gcgatactca
     3721 gggcatgctg ggagtgaaat atagctggta aaccgtataa gccgcatgtc gagatggcat
     3781 gcggcttaat attgccgact tcaaacggcg catcaatgcc ttatttaaat cctccttttt
     3841 atccgcgatc gcggatatcg cagcgtttat cccgtagagc ggataagatg tgtttccaga
     3901 ttgacttatc ctcactaaag gataaaactt ataatatccc ttaagcggat aaacttgctg
     3961 tggacgtatg acatgatgag ctttcagaag atctatagcc caacgcaatt ggcgaatgca
     4021 atgaaactgg ttcgccagca aaatggctgg acgcagagcg agctggcgaa aaaaattggt
     4081 attaagcagg cgacgatttc caatttcgaa aacaaccctg acaataccac gctcacgaca
     4141 ttttttaaga ttttacagtc gcttgaactc tcaatgacgc tatgcgacgc gaaaaatgcc
     4201 tcgccagaat caacagaaca gcaaaatctg gagtggtaat gcctaaactt gtcacttgga
     4261 tgaacaacca gcgggtaggc gagttaacga agttagccaa tggcgcgcac acctttaagt
     4321 atgcaccgga gtggttagcc aaccgttatg ccagaccgtt atcactttcg ctgccattgc
     4381 aaagggggaa tatcacctct gatgccgtat ttaacttctt cgataacctg ctacccgata
     4441 gcccgattgt acgtgaccgg atcgttaaac gttatcatgc taaatccaga caaccgtttg
     4501 atttattgtc agaaataggg cgagatagcg ttggtgccgt aacgttatta cccgaaaatg
     4561 aaaccataac gcgcccgata atggcatggg aacagctcac tgaagccagg cttgaagaag
     4621 tattaacggc ttataaagca gatatccctc taggcatgat tagagaagaa aatgactttc
     4681 gcatctcggt tgctggagcg caggagaaga cagcgctgct cagaataggc aatgactggt
     4741 gcattccgaa aggaattacg ccgacgacgc acatcattaa attaccgatt ggcgaaatca
     4801 ggcagcccaa tgcgacgctc gatctcagcc aaagcgttga taatgagtat tactgtctgc
     4861 tgctggcgaa agaacttggg ttgaatgtgc cggacgcaga aatcattaaa gcgggaaatg
     4921 tgcgcgcgtt agcggtcgaa cgttttgaca ggcgttggaa tgctgagcga acggttttac
     4981 ttcgcttgcc acaggaggat atgtgtcaga cattcggttt accttcatcg gtgaaatatg
     5041 aatcagatgg aggaccaggc atcgcgcgga tcatgacctt tttgatgggg tccagcgagg
     5101 cgctgaaaga tcgctatgat tttatgaagt ttcaggtgtt ccagtggttg attggcgcaa
     5161 cggatggcca tgcaaaaaac ttctccgtat ttattcaggc tggcggcagt tatcgactca
     5221 cgccatttta cgacatcatt tcagcatttc cggtattggg tggcgcagga atacacatca
     5281 gcgatctcaa actggcaatg gggcttaacg catccaaagg caaaaaaacg gcaatcgata
     5341 aaatttatcc gcgacatttt ctggcgacag caaaggtgct gaaatttccg gaagtgcaga
     5401 tgcatgaaat cctaagtgac tttgccagaa tgattccggc agcactggat aacgtgaaga
     5461 cttcattacc gacagatttt ccggagaacg tggtgacggc agttgaaacc aatgtgttga
     5521 ggctgcaccg tcggttaagc cgagaatacg gtattaagta agatatgggg tttggccgtt
     5581 gttaatgagc aatcatgacc gccaacgact ggattctccc ttggtttatg ttggtgcgca
     5641 taatgtgttg aacatgttaa atgactgcac gaagtgctgg catttagcct tctgcaatga
     5701 accgtcagac gttgtcactt gttttctggc atagaatatc actgcaacag cggaattaca
     5761 gatacatcct gaatgttcgt tttttcttat taatagatag cattgtgaaa tgccggttat
     5821 tttatgacta tttatagcag catcaacagg ttgtagcaac acagtttatg tcgggatttc
     5881 tgttttccca ctgaagtatg agccagcgca ggtaatgaac ataaacctgt acatcaccat
     5941 caatcatgta gcaattacgc tgaatctttt ttgtgagagg ccagaacaac actgtaccat
     6001 aaggagtttc agacaggatt actttcatat tcccgtcaag aacaacttca taatgaattt
     6061 gattgttttt taattcatct tcagcctcca ttaatatctc atctcgaatt ttatttattt
     6121 catgataatg cccggaagga atcttcatgg caacgcgtcg taatccagct tccctggcat
     6181 tttcttctgt gctgataaat gccaccgtgt cagcagaata aatccggcga tgtgagtaat
     6241 ccttatcgag ctcatactct cccggattat ccttccattt gttgtagcca tgcaattttg
     6301 gaaggtcatt gcagaagttc tgaaagttat gccatcgctc gcatacagtg acacctttgt
     6361 agcctttaaa atactgcttc cctttggttg tcatgtagca ccttgccagc atgttatgcc
     6421 agagcatgaa tgctctcgtt ctctggtgtg ttcgggtgtt ttcaatagtt gaatgacatc
     6481 cattaccaat atatccgacg ccgtaatatt ttggtgtcat cagtaacttt cgtcgttctt
     6541 cctccgatga tggaaaagct tcgtctgata cagaaccagt tttaatatgc gagccatagg
     6601 cttccttgat gatgccactg tctttgaatt ccacgacata atagcctcgc cttgatttat
     6661 catcagagcg gccaagaatg gcgcattctc cattatgtcg ggttctgaat gttgcgccgg
     6721 ggaacaatac gtcatgagta taattttgat gaaagcaatt attacggata ccttttttgt
     6781 agcggggcat aatatttctc ctgcgtgatt taactgaaag ggaaggg
//
LOCUS       DAJSKJ010000185         6815 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000185 DAJSKJ010000000
VERSION     DAJSKJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.187"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1946
                     /gene="acnA"
                     /locus_tag="ON345_003381"
     CDS             <1..1946
                     /gene="acnA"
                     /locus_tag="ON345_003381"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR3073391.1"
                     /translation="LIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVEFY
                     GDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYAKA
                     QGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELEVNAT
                     HKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVILGLKRQ
                     PWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLPDPIETAIK
                     KGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHDRK
                     GDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYGWQED
                     STYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRYLQ
                     GRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSIYD
                     AAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIGMG
                     ILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRID
                     TATELTYYQNDGILHYVIRNMLK"
     gene            complement(2010..2600)
                     /gene="ribA"
                     /locus_tag="ON345_003382"
     CDS             complement(2010..2600)
                     /gene="ribA"
                     /locus_tag="ON345_003382"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR3073392.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2770..3534
                     /gene="pgpB"
                     /locus_tag="ON345_003383"
     CDS             2770..3534
                     /gene="pgpB"
                     /locus_tag="ON345_003383"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR3073393.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3683..3991
                     /gene="lapA"
                     /locus_tag="ON345_003384"
     CDS             3683..3991
                     /gene="lapA"
                     /locus_tag="ON345_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR3073394.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARTERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            3998..5167
                     /gene="lapB"
                     /locus_tag="ON345_003385"
     CDS             3998..5167
                     /gene="lapB"
                     /locus_tag="ON345_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR3073395.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5360..6097
                     /gene="pyrF"
                     /locus_tag="ON345_003386"
     CDS             5360..6097
                     /gene="pyrF"
                     /locus_tag="ON345_003386"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR3073396.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6097..6423
                     /gene="yciH"
                     /locus_tag="ON345_003387"
     CDS             6097..6423
                     /gene="yciH"
                     /locus_tag="ON345_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR3073397.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6549..6767)
                     /gene="osmB"
                     /locus_tag="ON345_003388"
     CDS             complement(6549..6767)
                     /gene="osmB"
                     /locus_tag="ON345_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR3073398.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         1669 a         1609 c         1821 g         1716 t
ORIGIN      
        1 tgcttatccc ggacgtagtg ggctttaaac ttaccggtaa attacgtgaa ggtattaccg
       61 ccacagacct ggttctcact gttacccaaa tgctgcgcaa acatggcgtg gtggggaaat
      121 tcgtcgaatt ttatggtgat ggtctggatt cactaccgct ggcggatcgc gccaccattg
      181 ccaatatgtc gccagaatat ggtgccacct gtggcttctt cccaatcgat gctgtaaccc
      241 tcgattacat gcgtttaagc gggcgcagcg aagatcaggt tgagttggtc gaaaaatatg
      301 ccaaagcgca gggcatgtgg cgtaacccgg gcgatgaacc gatttttacc agtacgttag
      361 aactggatat gaatgacgtt gaagcgagcc tggcagggcc aaaacgccca caggatcgcg
      421 ttgcactgcc cgatgtacca aaagcatttg ccgccagtaa cgaactggaa gtgaatgcca
      481 cgcataaaga tcgccagccg gtcgattatg ttatgaacgg acatcagtat cagttacctg
      541 atggcgctgt ggtcattgct gcgataacct cgtgcaccaa cacctctaac ccaagtgtgc
      601 tgatggctgc aggcttgctg gcgaaaaaag ccgtaattct tggcctcaag cggcaaccgt
      661 gggtcaaagc gtcgctggca ccaggctcga aagttgtttc tgactatctg gcaaaagcga
      721 aactgacacc gtttctcgac gaactggggt ttaaccttgt gggatacggt tgtactacct
      781 gtattggtaa ctctgggccg ctgcccgatc ctatcgaaac ggcaatcaaa aaaggcgatt
      841 taaccgtcgg tgcagtgctg tccggcaacc gtaactttga aggccgtatc catccgctgg
      901 ttaaaaccaa ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg gcgggaaata
      961 tgaatatcaa cctggcttct gagcctattg gtcatgatcg taaaggcgat ccggtttatc
     1021 tgaaagatat ctggccatcg gcacaagaaa ttgcccgtgc ggtagaacaa gtctccacag
     1081 aaatgttccg caaagagtac gcagaagttt ttgaaggcac agctgagtgg aaggaaatta
     1141 acgtcacacg atccgatacc tacggttggc aggaggactc aacctatatt cgcttatcgc
     1201 ctttctttga tgaaatgcag gcaacaccag caccagtgga agatattcac ggtgcgcgga
     1261 tcctcgcaat gctgggggat tcagtcacca ctgaccatat ctctccggcg ggcagtatta
     1321 agcccgacag cccagcgggt cgatatctac aaggtcgggg tgttgagcga aaagacttta
     1381 actcctacgg ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc ttcgccaata
     1441 ttcgcatccg taatgaaatg gtgcctggcg ttgaaggggg gatgacgcgg catttacctg
     1501 acagcgacgt agtctctatt tatgatgctg cgatgcgcta taagcaggaa caaacgccgc
     1561 tggcggtgat tgccgggaaa gagtatggtt caggctcaag ccgtgactgg gcggcaaaag
     1621 gtcctcgtct gcttggaatt cgtgtggtga ttgccgaatc gtttgaacga attcaccgtt
     1681 cgaatttaat tggcatgggc atcctgccgc tggaatttcc gcaaggcgta acgcgtaaaa
     1741 cgttaggact aaccggggaa gagaagattg atattggcga tctgcaaaat ctacaacccg
     1801 gtgcgacggt tccggtgacg cttacgcgtg cggatggtag ccaggaggtc gtaccctgcc
     1861 gttgtcgtat cgacaccgcg acggagttga cctactacca gaacgacggc attttgcatt
     1921 atgtcattcg taatatgctg aagtaacaag tatttgcttg ccggttattt tgcttccggc
     1981 aagcaaatta attccacaat gcgagagggt tatttgttca gcaaatgacc cattttctcg
     2041 gctttggtat cgagataatg ttcgttattg gggttacgac ctacaatcaa tggtacgcgt
     2101 tcaacaatat taatccctgc ttcggtcaga atttcgactt ttttcgggtt attggttaac
     2161 aagcggactt cattgacgcc aaggagtttg aacatatcag cgcaaagagt gaagtcgcgc
     2221 tcatcagcgg cgaagcctaa ctggtggtta gcctctacgg tatcgtaacc ttgatcctgc
     2281 agtgcgtaag cgcggatttt attcagcaga ccaatgttac gaccttcctg acggtgatac
     2341 agcaaaatac cacggccttc ctcggcaatt tgcgtcaatg ccgcttcgag ctggaagcca
     2401 caatcgcagc gcaagctgaa cagggcgtca ccggtcagac attcggaatg gacgcgcgca
     2461 agtaccgggg tatgcccgga aatatcgcca tagactagcg cgacatgatc gtgtccggtt
     2521 gccagttctt caaatcccac catcaggaaa tcgccccatg gggttggcag tttggcttct
     2581 gccacacgtt taagctgcat gaaattctcc agataatgct ggttctgtat tggcttattt
     2641 tgccataacg agaaggggtt cacctaatca cgacgcgtcg atcgttcacg gaatggcaca
     2701 aatctgtcaa tttttcctgg aactggcgtt ttcagttatg attgtgggac ttatcaaaaa
     2761 ggagaggcca tgcgttcgat tgccagacgt accgcagtgg gagctgcact attgcttgtc
     2821 atgccagtag ccgtatggat ttctggctgg cgttggcaac ctggagaaca aagttggcta
     2881 ctaaaagcgg ctttttgggt tactgaaact gtcacccagc cctggggcgt cattacacat
     2941 ttgattttat tcggctggtt tctctggtgt ctgcgttttc gcattaaggc tgcctttgta
     3001 ttatttgcca ttctggcggc cgcaatcctt gtgggacaag gcgttaaatc ctggatcaaa
     3061 gacaaagtcc aggaaccacg accttttgtt atctggctgg aaaaaacaca tcatattccg
     3121 gttgatgagt tctacacttt aaagcgagca gaacgcggaa atctagtgaa agaacagttg
     3181 gctgaagaga aaaatatccc acaatatttg cgttcacact ggcagaaaga gacggggttt
     3241 gcctttcctt ccggtcacac gatgtttgct gccagttggg cactgctggc cgttggtttg
     3301 ctgtggccgc gtcggcgaac gttaaccatt gctatcttgc tggtctgggc aacgggagtc
     3361 atgggaagcc gcctgctgct cgggatgcat tggccacgcg atctggtagt agctacgttg
     3421 atttcgtggg cgctggtggc ggtggcaacg tggcttgcgc aacgaatttg tgggccatta
     3481 acaccacctg cggaagaaaa tcgcgaaata gcgcaacgag aacaagaaag ttaacgctgg
     3541 ttgattttcc gaatttagcc cttaaatcat caacaatgcg cgtggatgcc atttcgcaga
     3601 cggcgcgaaa atggtacttt aaagggctat tgcggtaagt tgaccataat ttattcgctc
     3661 taaccacata acgggaagta atgtgaaata tttactcatt ttcttactgg tgttagcgat
     3721 cttcgtgatt tcggtcacgt tgggtgcgca gaacgatcaa caggtgacgt ttaattatct
     3781 gttagcgcaa ggggagtacc gtatttccac attgctggcg gtattgtttg ctgcggggtt
     3841 tgctatcggt tggttgattt gtggcctgtt ctggctgcga gttcgtgttt ccctggcgcg
     3901 cactgaacgt aaaataaagc gactggaaaa ccagctttca cccgcgactg acgtggctgt
     3961 agtgccgcac tcgtcagcgg cgaaggaata actttctatg ctggagttgt tgtttctgct
     4021 tttgcctgta gccgctgcct atggctggta tatgggccgc agaagtgcgc aacaaaacaa
     4081 gcaagatgaa gccaaccgct tgtcgcgtga ttacgtagcg ggggttaact tcctgcttag
     4141 taatcaacag gataaagcgg tagacctgtt tctcgatatg cttaaagagg atacaggcac
     4201 cgttgaagcc caccttacgc tcggaaacct gttccgttcg cgtggcgaag ttgatcgcgc
     4261 tattcgcatc catcagaccc taatggaaag cgcctcgctg acctatgaac agcgtctgtt
     4321 ggcgattcaa caactagggc gtgattacat ggccgccggg ttatacgacc gcgcggaaga
     4381 catgttcaat cagctgaccg atgaaactga cttccgcatt ggcgctctgc aacagttgct
     4441 acaaatctac caggctacca gtgagtggca gaaagcaatt gatgttgccg aacgcctggt
     4501 gaagctgggt aaagataaac agcgcgtcga aattgcccat ttctactgtg agttagccct
     4561 gcagcatatg gccagcgacg atctcgatcg tgccatgacc ttgctaaaaa aaggggcggc
     4621 ggcagataaa aacagcgccc gcgtatccat catgatggga cgcgtgttta tggcgaaagg
     4681 agaatacgcc aaagccgtcg aaagtttgca acgcgtgata tcccaggaca gagaactggt
     4741 cagcgaaacg ctggaaatgt tgcaaacctg ctatcagcag ttgggtaaaa ctgccgaatg
     4801 ggcagaattc ctgcaacgcg cggtggaaga gaacaccggt gccgatgctg aattgatgct
     4861 tgctgatatc atcgaagcgc gcgacggtag tgaggccgca caggtctata ttacgcgcca
     4921 gcttcagcgt catccgacca tgcgtgtgtt ccataagtta atggattacc acttaaatga
     4981 agcggaagaa gggcgtgcca aagagagcct gatggtgctg cgtgacatgg ttggcgaaaa
     5041 ggtacgtagt aagcctcgtt atcgctgcca gaaatgtggt tttaccgcat acaccctcta
     5101 ctggcattgt ccgtcttgtc gggcctggtc aaccattaaa ccgattcgcg gtcttgatgg
     5161 cctgtaattt ttaaaaaaat ccgactttag ttacaacata ctagttattg atgttccatt
     5221 gtgtcccggc aacgacggag cagaaaaagc ctgccagggg aaaaatcgca actgttaatt
     5281 ttttatttcc acgggtagaa tgctcgccgt ttacctgttt cgcgccactt ccggtgccca
     5341 tcatcaagaa ggtctggtca tgacgttaac tgcttcatct tcttcccgcg ctgttacgaa
     5401 ttctcctgtg gttgttgccc ttgattatca taatcgtgat gacgcgctgt cctttgtcga
     5461 caagatcgac ccacgcgatt gtcgtctgaa ggtcggcaaa gagatgttta cattgtttgg
     5521 gccacagttt gtgcgcgaac ttcaacagcg tggttttgat atctttcttg acctgaaatt
     5581 ccacgatatt cctaacactg cagcgcacgc tgtcgctgct gcagctgact taggcgtgtg
     5641 gatggtgaat gttcatgcct ctggtggggc gcgtatgatg accgcagcgc gtgaggcact
     5701 ggttccgttt ggcaaagatg caccgctttt gattgctgtg acagtgttga ccagcatgga
     5761 agccagcgac ctggtcgatc ttggcatgac actgtcacct gcagattatg cagaacgtct
     5821 ggcggcactg acgcaaaaat gtggccttga tggtgtggtg tgttctgctc aggaagctgt
     5881 gcgctttaaa caggtattcg gtcaggagtt caaactggtt acgccgggca ttcgtccgca
     5941 ggggagtgaa gctggtgacc agcgccgcat tatgacgcca gaacaggcgt tgtcggctgg
     6001 tgttgattat atggtgattg gtcgcccggt aacgcaatcg gtagatccag cgcagacgct
     6061 gaaagcgatc aacgcctctt tacagcggag tgcatgatga gtgattccaa cagccgtctg
     6121 gtctactcaa cggaaaccgg acgtattgat gagcccaaag cggcccctgt acgccctaaa
     6181 ggcgacggtg tggtgcgtat tcagcgtcag accagtggac gtaaaggtaa gggcgtttgc
     6241 ctgattaccg gtgtcgatct cgatgatgcc gaactgacaa aacttgcagc ggaactgaag
     6301 aaaaaatgcg gctgcggcgg agcagttaaa gatggagtta ttgaaatcca gggcgataag
     6361 cgtgatttat taaaatcact acttgaagcg aaagggatga aggtaaaact cgcaggcggt
     6421 taacataaaa agccacggat atatccgtgg ctttcgaata ttttactgtg cgtattattc
     6481 agttatttcg tactgaacag aatcaagcgt attataattg cgagtcgata atcgacattt
     6541 atttacgatt atttaccgac ctggtgacca ataacaccac cgacagctgc accacctaat
     6601 gtacccaacg tactgccatc ggtcagtact gcaccgccta atgcccctgc acccgcgccg
     6661 attgcggtgt tgcggtcccg tttagaccag ttagaacagg cactcagaga cattgccaaa
     6721 gtaattgcca gaacagccgc ggtcattttt ttgctcgtta caaacataat actctctcct
     6781 gaatttatga ttcacggaag taagctctct ataac
//
LOCUS       DAJSKJ010000186         6777 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000186 DAJSKJ010000000
VERSION     DAJSKJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.190"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ON345_003389"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ON345_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR3073399.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="ON345_003390"
     CDS             2089..2238
                     /locus_tag="ON345_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073400.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMTQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="ON345_003391"
     CDS             complement(2983..3423)
                     /locus_tag="ON345_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR3073401.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="ON345_003392"
     CDS             3641..4030
                     /locus_tag="ON345_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073402.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="ON345_003393"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="ON345_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4668..5141)
                     /locus_tag="ON345_003394"
     CDS             complement(4668..5141)
                     /locus_tag="ON345_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR3073403.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            complement(5162..5998)
                     /locus_tag="ON345_003395"
     CDS             complement(5162..5998)
                     /locus_tag="ON345_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR3073404.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5998..>6777)
                     /locus_tag="ON345_003396"
                     /note="aph(3'')-Ib"
     CDS             complement(5998..>6777)
                     /locus_tag="ON345_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR3073405.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1586 a         1741 c         1762 g         1688 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatgacgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatcgcctg gcacagcgga tcgcaaacct ggcgcggctt
     4621 ttggcacaaa aggcgtgaca ggtttgcgaa tccgttgctg ccacttgtta acccttttgc
     4681 cagatttggt aactataatt tatgttagag gcgaagtctt gggtaaaaac tggcctaaaa
     4741 ttgctgggga tttcaggaaa gtaaacatca ccttccggct cgatgtctat tgtagatata
     4801 tgtagtgtat ctacttgatc gatcaggctt ttgtatatct ccccaccacc tgaaacaatg
     4861 acatgatccg ttattttctt taggttggtt aaagcatctt taattgatgg aaagatcaat
     4921 acgttctcat tgtcagatgt aaaacttgaa cgtgttacga ccgcatactt tcggttgggt
     4981 aatgctccca ttgattcaaa agtcttgcgt ccaaccaaca gccattggtt ataggtaata
     5041 gctttaaaca ggagctgttc acctttggca ctccatggaa tatcagggcc attcccgata
     5101 actccattct tcgatatagc taccattagt gatagtttca caattcttcc tcagaggtta
     5161 actagtatga cgtctgtcgc acctgcttga tcgcggccgc gatagctaga tcgcgttgct
     5221 cctcttctcc atccgcgttc caagctgcgg aaaggcaccc ataagcgtac gcctggtcga
     5281 gcaggcgacg cggatcgacg tccagcgcac gagagaatgc gtccgccatc tgtgcaatgc
     5341 gtctaggatc gagacaaagg tcgtctctgt cagccggatc gtagaacata ttggcggcgc
     5401 caaagcccac ttcaccgacc agaccgacgg gatctatcac cagccagccg cgactggaga
     5461 acatgatgtt ttcatgatgc agatcgccat gtagcccacg cagttccgag gcattgctca
     5521 tcatttgatc ggctataatc gccgcgtgga cgtagtcagt ttgacaacct gcgttttgat
     5581 catcgcgcgc ccgctgaaac aaagctgcaa agcgatcccg gatcgggaga agggcagaag
     5641 gcaggggttc ctcagatgcg gcatacagct tcgccattag ttccgctgca atttcggtcg
     5701 cctggtagtc gccgtgctcg gcaacgatgt gagagagcat tcgctccccg gcatattcga
     5761 gcaacatcag attgttctca cgaccgagca accggactgc tcccctccca ttgcgccata
     5821 ccagatagtc ggccccgcgc agttcatcag caatgtcttc tataggtttc aatcccttga
     5881 cgattgcagg agtcccgtct ggcaatgaaa ctttccaaac gaggctggaa aaggtgtccg
     5941 caatgagaac aggttgcgaa acgtgccaat gagcaggaaa aacaggcggc atgaacatca
     6001 accccaagtc agagggtcca atcgcagata gaaggcaagg cgttcgcggt cgggggcttc
     6061 gatccccaat acattgaata ggacagcgaa ggcgcgctct gcttcatctg gcgctgccca
     6121 gttctcttcg gcgttagcaa tcatgagtgc caaatcggca tagcgatctg ctgttccgag
     6181 ccgcccaagg tcgatcagac ccgtgcattg aagagtttta gggtccacca tgaagttcgg
     6241 catgcaggga tcaccatggc aaacaaccat atcggtgcgc tcttggtcga gccgcaccgg
     6301 tagctctcgt tcgacacgag ccaaaagatc gagctgcggc gtactcttgt cctcgtccgg
     6361 taagaagtcg ggattgacgg cattgcggga caccacatca acggcgcgtc cgaacattcg
     6421 cgacagcctg cgctcaaacg gacattgatc aaccgatagg ctgtgaacag cgccaagttg
     6481 ctgccccatt gacggccacg ctttgagcaa atccgctcca gacagatcag ccgccggtac
     6541 tcccggaatt gccgttatca ccaagcatgc accctcctgt tcctcctgcc agttgatgac
     6601 ctcggggcaa gccacacctc gacctttgag ccaaatgagg cggtcacgct ctccagcgag
     6661 ctcaccgcgg cgggaagcag gtgcgatttt cgcgaaggca tgcccgtcac cacgtcgaaa
     6721 aacaaaatca ccagattctc cgcctctgac aggcaaccag tcagaatgcg attcacc
//
LOCUS       DAJSKJ010000187         6705 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000187 DAJSKJ010000000
VERSION     DAJSKJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.191"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(93..578)
                     /gene="sixA"
                     /locus_tag="ON345_003397"
     CDS             complement(93..578)
                     /gene="sixA"
                     /locus_tag="ON345_003397"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR3073406.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(781..2925)
                     /gene="fadJ"
                     /locus_tag="ON345_003398"
     CDS             complement(781..2925)
                     /gene="fadJ"
                     /locus_tag="ON345_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR3073407.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2925..4235)
                     /gene="fadI"
                     /locus_tag="ON345_003399"
     CDS             complement(2925..4235)
                     /gene="fadI"
                     /locus_tag="ON345_003399"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR3073408.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4415..4699)
                     /locus_tag="ON345_003400"
     CDS             complement(4415..4699)
                     /locus_tag="ON345_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR3073409.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5071..6411
                     /gene="fadL"
                     /locus_tag="ON345_003401"
     CDS             5071..6411
                     /gene="fadL"
                     /locus_tag="ON345_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR3073410.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1652 a         1822 c         1601 g         1630 t
ORIGIN      
        1 gtcttcgata gtagtgaggt tgacagatat ccgctgcctg atgagcaatt atgcccaatg
       61 aatctacctc attgggcata atttggaaca catcagatag cttttgccat cttcagatta
      121 cacgggctca tctgccagtt gaatgtaccg tttccgcttt catcaagcgt tacgctggca
      181 atggccgagg tggtaaacat aggcggcgtt tcgcccggac ataactcggc aaccagatac
      241 ccgactaacg gtaagtgaga gatcaccagt accgaagcaa caccttcatt ggtcaacgcc
      301 tgcaaatagg cactgaccag accaacatcg ccgcagggcg ttaactccgg cagaacttct
      361 gcactggaag gcaggttcag acaatcccct acttcttcca gtgtttgctc ggctcgcagg
      421 aacgggctca ccagaacgcg ttcgatttcc actttttgac ctttcagcca gttcgccatc
      481 aggcgagatt cgtcacaacc attagtggtc agaggacgaa cggaatcact ggcggcatcg
      541 agggctgcgt cgccgtgacg catgataaaa acttgcatat tgcaccgctt ttgttaacca
      601 gattcgcccg ttttctttca tctgaaacca gagaaagaaa acggtggccg gcattgtgcc
      661 ttatccattc accgaatgaa acgctgtttt ttacctcaat ggcgtaagta tagtcaatat
      721 gcgtttacat tttgaccaac actccgccat tcagcgcgga ttcatatagc tttgaccttc
      781 ttattgcagg tcagttgcag ttgttttcca aaaactttcc ccacgcgcgc ccatctcgac
      841 caaacgctcg caaggggtaa aacgggaacc atactgcgtg gcaagtcgtt gcattattgc
      901 aaccacttcg cccgcgccga gagaatcgat atagcggaac ggtccaccga gaaatggcgg
      961 aaaaccaatg ccaaataccg cgccaatatc cccgtcacgc acgctacgga taacctgctc
     1021 atcaacacaa cgtactgctt cattcagcat caacatcaca caccgttcag caacctgcgg
     1081 tgcggagagt cgcccctgcc cttgtgcgcc aatgagcggg taaatggcag gatcgacctg
     1141 ttttttgctt ttacgccctt tctgaccata aagatagaaa ccccgaccat tttttctgcc
     1201 tttgcgatcg tcgttcaaaa ttgaagaaac aacatttgca ggcgcgctaa aacgttctcc
     1261 ataagcggct tccagtacag gaataatttt agtcccggtg tcgattccta cctcatccaa
     1321 aagttggatt gggcctaccg gaaaaccaaa tttcactagc gcagcatcaa tgtgctctac
     1381 ccgttcacct tcggtcagca tgcggatagc ttcattaatg taaggcgcta agatgcgatt
     1441 gacgtaaaac ccggctttgt cacgcacgac aatgggcgtt ttgccctgtt ttttcgccag
     1501 ttttaccgta gtggcgatgg tttgcgctga tgtacccgca tgaggaataa tctccaccag
     1561 cggcattttt tccaccggac tgaagaaatg caggccgata acttgctcag gtcgcgtggc
     1621 gtgagcggcg atatcaccaa tcggcaaaga tgacgtattc gaagcaaaga tggtatgagc
     1681 ggcgcaattt tgctccactt ccgccaccat ctgttgtttt aattcgagat tttcaaacac
     1741 cgcttcaata atcagatcgc gatgggcaaa gccgcgatag tccgtcgttc cggagattaa
     1801 tgccagctgt ttgtcacgtt cgctggcttt gagatgacga cggcgaactt tgccctccag
     1861 ctgatcccaa ctgtacttca gcgcatgatt tatgccctgc gggttgatat ctttaattct
     1921 gaccggaagc cccgctttac aagcagtgac ataagcaata ccgccgccca tcaagccacc
     1981 acctaaaatt cccacgctgt ttaatggcgc aggcggtgca tcactaccgg gatctttctt
     2041 cacttccgta ctggcaaaaa agatactacg cagcgcctgc gattgtggcg tcatcgccag
     2101 ttcgccaaac gcccgagctt cggcgtcata accgctgctg gtgccctgcg ctaatcccgt
     2161 ttcaacaacc tccaggatgc gttctgtcgc cggataattg ccttgagttt tgtgttctgt
     2221 tttcttgccg accattttga acagcagcgc acgacctaac ggccccgcca gaatacgctc
     2281 gcgtacaggt agagggcggg aagatgggcg atccttcttc gccagctcaa cagcgacttc
     2341 cagcaaaatg gagtgcggaa caacgtcatc caccagcccc agctttaatg cctgtttcgc
     2401 ccgaagttgt tttccggtga gaatcatctc taatgctgta ctgacgccta tcagacgcgg
     2461 taaacgctgg gtgccgcctg aaccgggtaa caatccaagt tgtacttcag gcaaaccgag
     2521 caccgtttta ggatcgtcag tacaaacgcg accgtggcac gccagcgcca gctccagccc
     2581 accgcccagg caagcgccat gaatagccgc gataaccgga atgggcaaag catgaatctc
     2641 cgccatcaac tgttggccct gccgcgccag tgcttccgct tcttgcgccg ttttgcagtt
     2701 gccaatcatg ttgatgtctg ctccggcgat aaaattgtcc ggtttcgcgg agataaacac
     2761 cacgccgcgc agctctttgt tttcacggag ttgcttaata atggcgcgca cctgcgaggc
     2821 aaattccgcc ttcagggtat tcattttctc atccggtacg tcgatggtga tgatagcaat
     2881 gttgtccaga cgaacattaa gagtaaacgc tgatgccatt tccattattc cgcctccaga
     2941 accattgccg cgccaagccc accggcagca caggcggtaa ctaaaccaaa tccaccgccg
     3001 cgacggcgaa gttcatgcaa tgtctgggta atcatccgcg cgccggtcgc cgcgaaggga
     3061 tgtccgtaag caatcgaacc gccaagcacg ttaaatttgc tatcgtccac ttcgccagtg
     3121 gcatgtgcac gccccagtac ttcacgagca aaacgttcac tacccagcaa ctgaatattc
     3181 gccagtgtct gagctgcaaa ggcttcgtgc atatcgatca atgtcagttc gctcatcgtc
     3241 aaaccggcac gttccagcgc cagcggtgtt gaccaggctg gaccgagcaa catgtcctgc
     3301 cagacatcaa tcgcagtaaa tgcgtagctg cgcagatacc ccagcggcac cagccctaat
     3361 tctttcgccc gggattcagt catcaggatc accgctgccg caccatcggt cagcggcgta
     3421 ctgtttgccg ccgttaccgt tccgtgtttg cgatcaaacg ccgggcgcag ctttgcgtaa
     3481 tcggcaagcg aggaattacc gcgaatattg ttgtcttcga caagcggttg tttataagga
     3541 gggataaagg cagtcatcac ctcttctttg agttttccgt ctgaccatgc ctgagcggca
     3601 cgctgatgcg aacggtgcgc taatgcatct tgctgttctc gggtgatgcc gtaggttttc
     3661 gccatttgtt cggcggtgtc gcccatccgc aagccagtag aatattctgc taccgcaggc
     3721 ggtacgggca ttaagtcgcg caaacgcagg cgagagaaga gtttcaggcg ctggctcatg
     3781 gttcgagctt tgttgacatc aaccagcacg cgcgccagtt ttttactgac gccaattggc
     3841 aatacggaag aggaatctgc cccaccggca atccccgctc ggatagttcc cgccatcagg
     3901 ctttctgcga cgtttgcaac cgcctggaaa ctggtagcgc aagcgcggct gacgctgtaa
     3961 gcatcggtat gcacattcat tccggtaccg agaacaattt cacgcgcaat gttgggggct
     4021 tcaggcattt gtacgacctg accaaagacc agctgttcaa tcacttcagc ggggatctcg
     4081 ctgcgtgcca gcagttcgcc taccaccatc ttccctaaat caaccgcggg aatgccatga
     4141 aaagccgtcg cctggcgggc aaaaggcgta cgtaaaccgc taacaatggc gatacgatcg
     4201 ccctggcggg taaccagcgg taaaacctga cccataacac tcccctgtaa aaaaataaat
     4261 aaagtggtct gacctgatca cagtcttaac cattttttta catttagcca agcggagaaa
     4321 aaggaaagtg ggagctatga cacagtgaag acaggagaaa gaaaagaaaa cgcccctgcc
     4381 agcaactgac aggggcgaac gtacccgaga ggaattaacg cagacccagc tggaagatca
     4441 gcatttcagc ttcgcaggca aaagtgaaat cgatatccag gcgcacaccg tcagactctt
     4501 cagtgaaagt cggggagatt ttgcacggtt cggattccac gctacgggct ttttcagtca
     4561 gcgccgccag cgtttgttct gcttctgcgc ggtttgcaaa cacgcggctg taagacgcgg
     4621 tgcagtcgga gttgtccata atggtgccaa catccataca gcagcaaacc ggggtttcat
     4681 cagcactaca tttactcatc gttgatttcc tctgtatgtg cacccaaggt gccagataaa
     4741 cgttgtggat attttacgct tccggaaagt gctgctccag ttgttaattc tgcaaaatcg
     4801 gataagtgac cgaaatcaca cttaaaaatg atctaaaaca aaattcaccc gaatccgcga
     4861 gtgcgcaacc tccaaatttt gccagctgga tcgcgtttct tagatcatat ttgaaaaaag
     4921 atggaaacat acttgcaaca ttccagctgg tccgacctat actctcgcca ctggtctgat
     4981 ttctaagatg tacctcagac cctacacttc gcgctcctgt tacagcacgt aacatagttt
     5041 gtataaaaat aaatcattga ggttatggtc atgagccaga aaaccctgtt tacaaagtct
     5101 gctctcgcag tcgcagtggc acttatctcc acccaggcct ggtcggcagg ctttcagtta
     5161 aacgaatttt cttcctctgg cctgggccgg gcttattcag gggaaggcgc aattgccgat
     5221 gatgcaggta acgttagccg taaccccgca ttgattacca tgtttgaccg cccgacattt
     5281 tctgcgggtg cggtttatat tgacccggat gtgaatatca gcggaacgtc tccgtctggt
     5341 cgtagcctga aagccgataa catcgcgcct acggcatggg ttccgaacat gcactttgtt
     5401 gcaccgatta acgaccaatt tggttggggc gcttctatta cctctaacta tggtctggca
     5461 acagagttta atgatactta tgcaggcggc tctgtcgggg gtacaactga ccttgaaacc
     5521 atgaacctga acttaagcgg tgcgtatcgc ttaaataatg catggagctt tggtcttggt
     5581 ttcaacgccg tctacgctcg cgcgaaaatt gaacgtttcg caggcgatct ggggcagttg
     5641 gttgctggtc agattatgca atctcctgcc gggcaaactc ctcaagggca agcattggca
     5701 gctaccgcca acggtatcga cagtaatacc aaaatcgctc atctgaacgg taaccagtgg
     5761 ggctttggct ggaacgccgg aatcctgtat gaactggata aaaataaccg ctatgcactg
     5821 acctaccgtt ctgaagtgaa aattgacttc aaaggtaact acagcagcga tcttaatcgt
     5881 gcgtttaata actacggttt gccaattcct accgcgacag gtggcgcaac gcaatcgggt
     5941 tatctgacgc tgaacctgcc tgaaatgtgg gaagtgtcag gttataaccg tgttgatcca
     6001 cagtgggcga ttcactatag cctggcttac accagctgga gtcagttcca gcagctgaaa
     6061 gcgacctcaa ccagtggcga cacgctgttc cagaaacatg aaggctttaa agatgcttac
     6121 cgcatcgcgt tgggtaccac ttattactac gatgataact ggaccttccg taccggtatc
     6181 gcctttgatg acagcccagt tccggctcag aatcgttcta tctccattcc ggaccaggac
     6241 cgtttctggc tgagtgcagg tacgacttac gcatttaata aagatgcttc agtcgacgtt
     6301 ggtgtttctt atatgcacgg tcagagcgtg aaaattaacg aaggcccata ccagttcgag
     6361 tctgaaggta aagcctggct gttcggtact aactttaact acgcgttctg ataacgcgtt
     6421 cgcctggata aagtcacctg catagcaggt gactttaact cccccacttc accgaaagta
     6481 gtgtcctcat tgcttacctc ctgagttttg caaacagcct gttggcagct tgccctttca
     6541 aatcaataag cggtatccac agacaaacct gaagggaaag gcatattttc aggcgttctg
     6601 ctcgtccttc tcaaagagtt tacttttctg catttccagg atactccccc ccctggctat
     6661 tgtgcgctca tacactcaaa ttaaagatag gttctaaata aatga
//
LOCUS       DAJSKJ010000188         6612 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000188 DAJSKJ010000000
VERSION     DAJSKJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.192"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(257..1948)
                     /gene="fruA"
                     /locus_tag="ON345_003402"
     CDS             complement(257..1948)
                     /gene="fruA"
                     /locus_tag="ON345_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR3073411.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(1965..2903)
                     /gene="fruK"
                     /locus_tag="ON345_003403"
     CDS             complement(1965..2903)
                     /gene="fruK"
                     /locus_tag="ON345_003403"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3073412.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPELKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2903..4033)
                     /gene="fruB"
                     /locus_tag="ON345_003404"
     CDS             complement(2903..4033)
                     /gene="fruB"
                     /locus_tag="ON345_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR3073413.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4401..5582
                     /gene="setB"
                     /locus_tag="ON345_003405"
     CDS             4401..5582
                     /gene="setB"
                     /locus_tag="ON345_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR3073414.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     SLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(5579..5833)
                     /locus_tag="ON345_003406"
     CDS             complement(5579..5833)
                     /locus_tag="ON345_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR3073415.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            5988..6560
                     /gene="yeiP"
                     /locus_tag="ON345_003407"
     CDS             5988..6560
                     /gene="yeiP"
                     /locus_tag="ON345_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR3073416.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
BASE COUNT         1521 a         1840 c         1640 g         1611 t
ORIGIN      
        1 ctcacaaaat gcgattaaat gcctgaatta caaaagcgag gaatttgtga cgggatgcac
       61 agaacgggaa caataaaaaa ggcagaaaat aatctgccct tgaggaaata accttagtgt
      121 ttgatgaacg tttcaggttt ctactgaaag ataaaaccat ttaaattcat aaaattacat
      181 tcacaaaccg gtcattctct ttgcgctcat caaatgttac aggacaggaa atttctgccc
      241 tgtaacacac cttttattac gctgctttcg ctactgcgtc cacttccgga cgtttcagga
      301 aggcataggc caaacccgcc accagcgtac cggcaataat tgctaccagg taacccaata
      361 ccggcgtaat cgcgccaggg atcagcagaa caaacagacc accgtgcggt gccatcagtt
      421 tcgcaccaat cgccattgag attgcgccag tcagcgcccc acccacgata cagcacggca
      481 gcacacgcat cggatcacga gcagcaaacg gaattgcacc ttccgaaatg aagcacagtc
      541 ccaataccag agcggctttg ccaccttcct gctgcgcttt gtcgaatttg cgacgcgcca
      601 ccattgttgc cagacccatt gccagcggtg gcaccatacc tgccgccata atcgccgcca
      661 tcgggccata ggtttgagta ctcagcagac ccacaccgaa tgcgtacgct gctttgttta
      721 ccggaccgcc catgtcagta cacatcatgc caccgaggat cgcccccagc agaaccgcat
      781 tcgcagtccc catggtctgc agccagtgag tcagcccttc gagaatgcca gcaaccggtt
      841 taccgatcag gtagatcatc gccagaccga ccaccagact ggaaattagc ggaatgatca
      901 ggatcggttt cagcgcctcc atactctgtg gcagtttcaa ttgcgtactg attaacttcg
      961 caatgtaacc agccaggaag cccgcaataa taccaccaat gaagccagaa ccggtgctga
     1021 ccgccagcat accgccaatc agacccggcg tgaggcccgg acgatcggca atggaaaagg
     1081 caatataacc tgccagtacc ggcaccatca gcgcaaaggc tgaaccacca ccaatctgca
     1141 tcagcgccgc tgccaacgta cccggctctt taaacgcttc gataccaaaa gcaaaagaaa
     1201 gcgcgataca cagaccacca gcaacgacca tcggcagcat gtaagagacg cccgttagca
     1261 agtgacgata agcgcctgca ctctctttct taccttcagt ggtcgccgtt tgagctttgc
     1321 ccgccggttc atacggcgtt gcttcagcaa ccgctttatc cagttcctgc gcggttttct
     1381 tcagcgccag accggtagag gtacgataca tcggtttacc agcaaatttc gccagatcca
     1441 cttcgatatc tgccgccaca atcaccagat ccgctgcggc tacttcctcg ggagtgattg
     1501 cattacccgc gccaacagaa ccacgggttt caactttcac ccaccagcca cgttttttcg
     1561 cttcggtttc aatggcttca gccgccataa aggtgtgtgc tacgccagtc gggcaagcag
     1621 tcaccgcaac tacgcgtttc ggaccgctgg cggcaaccgg tgctgtcgca gtgaccggcg
     1681 cagtgtaagg tttcgcatgg cctttggctt cactcaggaa cagctcaggg tgcgcaactg
     1741 cccgggaaat atcacccagc cagacatttt taccgttcag cgcgctgtca ttcgggatgg
     1801 aatcaccgag aacaatcgcc atttctgcgt cgttcggatt gtcgatgatt tccagttttg
     1861 cttttcgcgc cgccgcgccc agcagggtct tcgccatata ggcgcgtgcc tgaccgagat
     1921 tagcgtcaat aatcagcagc gttttcatta tgcctctcct gctgtcagtt aaaaggttgt
     1981 aagtcgacgc gcgccatcat tgcggccaac tgcggacgat cggtaatacc cacattgctt
     2041 tgacttaccg ccagggctgc aacagctgtc gccagacgca gtgtgtgttc actggattca
     2101 cgcatcagca agccataaat caggccacca accatagaat cccctgcgcc aacggtgctt
     2161 acgacatcga ctgacggtgg tttggcgatc cattcgccgg aggcattaac ccaaagcgcg
     2221 ccttcggcac ccagtgaaat aacaacatgc gcaatacctt gttcacgtag cgcatgtgca
     2281 gcttcaatca catctttcag ttcaggcagt ttacggcctg cccagatttc caactcgcga
     2341 cggttaggtt tcaccagcca cggtgccgct ttcaaacctg ctactaacgc ttcacggcta
     2401 ctatcaaaga taatgcaagg acactgacta cgcaggcgag tcatccagtc ggtgaacgct
     2461 tccgggctga cgcctgacgg taagcttccg ctgacacaga ccatatcgaa ctgaccgagc
     2521 cagctcagag aatcagtcac aaagcgttcc cagtcggcgg gggtgacttc aaaacccgag
     2581 aagttgaagt cggtcacttc gccgtctttt tccgtcagct taacgttaat tcgggtgcgc
     2641 ccctgtacaa cctggaaacg gttggcaatg cccagctcgc tgaacagttg ctgaaaacca
     2701 tcctgattgt ctttacccag gaagccgcca acggtgacat caattcccag gtcttttaat
     2761 actttggcca cgttgatgcc tttacccgcc gcatgcagac cggtggtttt caccaggttc
     2821 acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt cataagccgg attaagggtg
     2881 atagtagcaa cacgtctgct cattatgcgc cctccccaag accagcagcg atagcgtcgc
     2941 cgattgcttt cagcgcctgt tcagcatctg caccctgggc ggtaaagcgt aggcgatgac
     3001 ctttcttaac gccaagtgcc acaactttca tcagactacg tccgtttgcc ggtttgccgg
     3061 taccatcaag gtttgtcacg gtaatatcac tgttaaattg tttaatggta ttgaccagca
     3121 tggtacctgg acgagcatgc aggccgtgtt cattgcgcac cacaaactcc gcgcttaaca
     3181 cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag caacgttgcc gcatccgctt
     3241 tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc gagacgctta agaaccgcga
     3301 tgggctgatc gtcattcatc gccacactca ccagcatggc tgccgtttcg ccgtccacat
     3361 caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg cagattgcct tcggcgctat
     3421 cgctcagcca gataccctgt ccgagattca gcggttgttc attgatggct ttggtgacga
     3481 aagtggcgtc aactgccccc gcctctttca gacgcgcagc gttcagcgcc tgaagagtca
     3541 gcagatcgct ggcgacgata tccagcgtca gcatttcgtt gtcgagcttc agctgctcac
     3601 tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc tgttgttgct gacttcagtt
     3661 gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg gcgtagcagg cccagatgtt
     3721 catccgagct ggcagcaata ccgattgcca cgtacgctac ctgaccgtca ccccaggtga
     3781 cgccttccgg gaactgaaat acctgaacgc cggttttcag cacctgatcg cgggtgtcgg
     3841 tagtgccgtg tggaatagca ataccattgc cgaggaacgt tgaggtttgc tgttcgcgcg
     3901 ccagcatgcc attgacgtag ccttctgcta cattaccggc ctgcaccagc gccgcagcga
     3961 cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc cggatggatg tcctgtacgg
     4021 ataactggaa catagttctc ctctcttgct gaattgaaac gattcagcct ctatgagaaa
     4081 aaaagcgcca acctggctta gggttaaaga caagatcgcg ctgaaacgtt tcaagaaagc
     4141 ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt tatgagcaaa agtttgatgt
     4201 gctgcacaat tttttgccat ttttcctaat tgctgatggg aaaatccgtt atcagcaatt
     4261 tcatttcagc agcgtcagca gccaatcagc aacagtgaaa tggcattttg attattcgtg
     4321 gcgcttttga tttcgctgtc tgtttatttt ctgacaagca gcgtaaactc cgcgtcttcc
     4381 tcttccagtg atcgaccagc atgcataact cccccgcagt ctccagcgcg aaatcgtttg
     4441 acctgacctc gacggcgttt ttaatcgttg cctttctcac cggtattgcg ggcgctctgc
     4501 aaaccccgac actcagtatt tttcttaccg atgaagtaca tgcccgtccg gcgatggtgg
     4561 gattcttctt taccggcagc gctgtcattg ggattctggt aagtcagttt ctcgccgggc
     4621 gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt ttgctgcctg ttaggcgtgc
     4681 tggcctgcac cctttttgcc tggaatcgca actactttgt tttgctattc gttggcgtct
     4741 ttcttagcag ctttggctcg accgctaacc cgcaaatgtt tgcccttgcc cgtgaacatg
     4801 ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt tttacgcgct caggtttcac
     4861 tggcatgggt cattggccca tcgctggctt atgcgttagc gatgggtttc agctttacgg
     4921 taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg cgtgatggtg tggctgtttt
     4981 taccgtcgat gcaaaaagag cttccgctgg cgaccggcac ggttgaagcg ccgcgccgta
     5041 accgtcgcga tacgctactg ctgtttgtca tttgtacatt gatgtggggc tcgaacagcc
     5101 tgtacatcat caacatgccg ctatttatta tcaacgaact gcatcttccc gagaaactgg
     5161 ccggtgtgat gatggggacc gccgccgggc tggaaatccc gacgatgttg attgccggat
     5221 atttcgccaa acgtctgggt aagcgtttct taatgcgcgt tgctgccgtg ggtggcgtct
     5281 gtttttacgc aggaatgctg atggcgcatt cacctgtcat tctgttgggc ttgcagctgc
     5341 taaatgctat ttttattggc attctgggcg gcatcgggat gctctatttt caggatctga
     5401 tgcccggtca ggcgggttca gccaccacgc tctataccaa cacttcgcgc gtgggctgga
     5461 tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg gaattatcac gctgtgttct
     5521 ggtttgcgat ggtgatgatt atcgccactc tgttttgctt actgcggatt aaagatgttt
     5581 aaggcgcggt cagcatttcc agatcgataa gccaggtcat ggcctgctga cgcgagttac
     5641 cgcacatttc agcgctggct tgtaagcctg cacagacttt cggtcgcagt ggcgaggtga
     5701 aaattttaca gcgttgctgt tcatcaagct gaatgcaggg cgtattggcg ggcttgccat
     5761 ctggcatacc gggaataggg ctggaaattg aaggggcagt gcaacacgcc ccgcaacccg
     5821 gacggcattc catacgttgt tctcttaagg ttaaccaccg gattgatgag cgcgcacagt
     5881 accacctttt ttgcaccagc aaaagtgcga ataccacttg cccgaaaggc ccgtcgcgag
     5941 tactttgtcg cgatattttt gacattttcg actacaggaa tttttcgatg ccaagagcga
     6001 acgaaattaa aaaaggtatg gtactgaatt acaacggcaa actgctgctg gttaaggata
     6061 ttgatattca gtcgcccact gcccgcggtg ccgctacgct gtacaaaatg cgtttttctg
     6121 atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg tgatgatatc gttgacaccg
     6181 tgacgctgac ccgccgttac gttgacttct cctatgtcga tggcaacgaa tatgtcttta
     6241 tggataaaga agactatacc ccgtatacct tcaccaaaga tcagattgaa gaagagttgc
     6301 tgtttatgcc agagggcggc atgccggaca tgcaggtgct gacctgggac ggtcaactgc
     6361 tggcgcttga gctgccgcag accgttgatc tggaaatcgt tgaaaccgca ccaggcatca
     6421 aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt gagcactggt ctggtgattc
     6481 aggtaccaga atacttaagc ccgggcgaaa aaattcgtat ccatatcgaa gaacgccgtt
     6541 atatggggcg tgctgactaa cttcagccgc atgcagaaaa gggatagctc aggctgtccc
     6601 ttttttaatt ta
//
LOCUS       DAJSKJ010000189         6548 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000189 DAJSKJ010000000
VERSION     DAJSKJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.193"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..467
                     /locus_tag="ON345_003408"
     CDS             <1..467
                     /locus_tag="ON345_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073417.1"
                     /translation="HIYGGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDK
                     GFSCMDSLFATTNKLGEVANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNINFTDYLF
                     GSFTSNSSLILYKPEFERKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(523..1065)
                     /locus_tag="ON345_003409"
     CDS             complement(523..1065)
                     /locus_tag="ON345_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3073418.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1049..1360)
                     /locus_tag="ON345_003410"
     CDS             complement(1049..1360)
                     /locus_tag="ON345_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR3073419.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1545..2435)
                     /gene="cyoE"
                     /locus_tag="ON345_003411"
     CDS             complement(1545..2435)
                     /gene="cyoE"
                     /locus_tag="ON345_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR3073420.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2447..2776)
                     /locus_tag="ON345_003412"
     CDS             complement(2447..2776)
                     /locus_tag="ON345_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR3073421.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2776..3390)
                     /locus_tag="ON345_003413"
     CDS             complement(2776..3390)
                     /locus_tag="ON345_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR3073422.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3380..5371)
                     /gene="cyoB"
                     /locus_tag="ON345_003414"
     CDS             complement(3380..5371)
                     /gene="cyoB"
                     /locus_tag="ON345_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR3073423.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5393..6340)
                     /gene="cyoA"
                     /locus_tag="ON345_003415"
     CDS             complement(5393..6340)
                     /gene="cyoA"
                     /locus_tag="ON345_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR3073424.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHVESAH"
BASE COUNT         1772 a         1676 c         1613 g         1487 t
ORIGIN      
        1 tccacattta tggtggcccg gaagactttc tggctacttg tcgaaaagac atgattgaca
       61 gaaaaacggt tgacgatacg ttacccgtga ttacggttac ccgttctggg atgcgagata
      121 attttttaga taaaggtttt tcctgcatgg atagcctctt cgccaccacc aataaattag
      181 gcgaagtggc caatcttcgc gtgacattca gcattcgtcg cccatccggt aaggagatta
      241 accaaacggt tggcttcgcg cctttcggtt taaaccggct gaatattaac tttactgatt
      301 acctatttgg ttcatttacc agtaattcgt ctcttattct ttataaaccg gaatttgagc
      361 gaaaatcttg tgcaactgta aggaccacaa tcgttgccgc aacggcaacc attaacggca
      421 aggatgtgga gttgctgaaa gcgggggcaa ttgaacaaaa gtggtaacgc tgctcagccc
      481 ccggctgagg caaaacttgc cctggcgggt ggcgataata atctaagccg cgagaatatg
      541 ctgaatcgcc aaaaacatag gtacagcacc aaaagcattt ggtgtcgctg aaagctgaac
      601 aaagccgaga cgagcataaa aattaatggc ggcagggtca gcatcaagat aaaccccttt
      661 aattggtaat gcctggtgaa ttatttttac atgttcaaaa aaatcacata gtaggtcctg
      721 accgaagccc cgcttttgat acttccgtgc tacccccaac atgactaatc tgacaacacc
      781 aatttctgaa ggttgtgaac cctgaaggac gccagaaacg cgttgttttt ccagcgaata
      841 tgccgtaaaa gtacagatgc cgatcagttc accactttgt ctgtcaataa gtgctttagc
      901 cgcacagtcg ctattacgca cacttttctt tagcgatgcg cgtacaaatt tatcgataac
      961 agggttaccg caatcaaact gtttttgccc aggataagta atatcagact gataattaca
     1021 ggagaggact atgggcagag taatctcttc atgcttgtcg accatcgctg tttttccttt
     1081 tcattaacgc cttgagggcg agcgtcggtt gagcaggttc agcgatgagt tggttcatca
     1141 gttcccagct ttgattcgaa agctcacggc gacgttggtt atcaagcaca ctttcggcgc
     1201 gttccatagc agcgctcaaa ataaaagcgc ttaaatctac cccaacggct tgcgcagctt
     1261 cacgaagttt gtttttggcg tcgggactag ttttgagttc gacccgcgca tttttcagcg
     1321 ttttatcgac atcgggtaga gtggctgttt ttccaggcat tatgacctct ttgattaact
     1381 gaatgaaatc gtagggagtc gatctcatac ataatacggc tatcaaccgt atgattaatt
     1441 tatgttcatc tgctcactct gtcaagacat acggttatta gccgtatctc tgatctcaga
     1501 caataaaaaa ggtgccgtag caccttttta atagagaggt tttgttacca cacagcagcc
     1561 agcagcgtat gcgagtccgg taccataaaa tcaacggaca tcatcaccga gagggcagtg
     1621 atggcgatga tagagaagcc gaacagcttg cgcgcccaga ttctgtcatc agcaacttta
     1681 taaccgcgca gagccatacc taaccaccag acgctaaccg ccgcggcgac caccagatat
     1741 ttatatccag cgtaaccgcc aagagagagc atcagcgtgg caacggcaaa ggcgatgata
     1801 tacagcgtga tgtgattctt cgccaccgaa atgcctttta ccactggcaa taccggaatg
     1861 tttgccgcct ggtaatcctt aaagcggaaa atggcgatgg cataggagtg aggcatctgc
     1921 cacaggctga agatagccag caggatcgct gcgccgctat cgaactcgcc ggttactgca
     1981 cagtagccaa tcaccggcgg cgcagcgccg gagagcgaac caatcaatgt gccgtagact
     2041 gagtggcgtt tcatgtacag gctatagacg ccgacataaa ccacaaagcc catcaccccc
     2101 agccagcagg ccagcggatt cgcgccaaac cacagcagca taaagccagc aatacccagc
     2161 aaggtggcgt acaccagcga gacagcagga gagatcaggc ctttaaccag cacccgattc
     2221 ttcgtccttt ccatctttct gtcgatatcc ctgtcgatgt agttgttaaa cacacaaccc
     2281 gacgccacaa ccagtgacac cccaaccagc gtgtagataa acaggggata atcaatgctg
     2341 ccctttgagg ccagcaggaa tcccccaatc accgagatca ggttgccaaa gatgatgcct
     2401 ggtttcgtta cttgcaggta ttgcttaaac atcataaccg ccgctcttag tgcatcatca
     2461 tgttgtagtt gaggttccac ataatccaga tggagcctac aaccaggata gcgatgatta
     2521 gcacggtgaa gacaaacgcc gtcatgttcc agccttcatc tgatttggta ttcatgtgca
     2581 ggaagcacac cagatgcacc agaacctgta ccactgccat tgccaggatt gttcccagaa
     2641 ttacggccgg agaggcagcc cctgtcatca ccatccagaa cggaatcacc gtcagaatga
     2701 tcgacaggat aaagcctgtc atgtaggttt ttacgctgcc atgggacgcg ccgctgtgat
     2761 cggtagaatg actcattaca tcgcccccat cagataaaca acagtgaaca cacagatcca
     2821 aaccacatcc aggaagtgcc agaacaggct caggcacatg atgcgggtac ggttagtgct
     2881 ggtcaggccg cgacgagcga tttgtaccat cagcaccgcc atccagataa gaccggaagt
     2941 gacgtgcaga ccgtgcgtgc cgaccagcgc aaagaacgct gacaggaagc cgctgcgatc
     3001 cggacccatg ccgttaacaa tcaggtgatg gaattcgtag atttccatcc cgataaatcc
     3061 ggcaccaaac aaccaggtca acgccagcca ggagataacc tggcttttgt tgtttttgta
     3121 catggcgata gccgccatgc cgtaggtgat ggagctgaac aacagcaaga aagtttcaac
     3181 cagaacgaac ggcagttcga aaatgtcctt acctgtcggg ccgcctgcgg tgccgttcac
     3241 cagaacggca taggtagcaa acaagattga gaacagaatg cagtcgctca tcaggtagat
     3301 ccaaaatccg aagattttgg ttccgcctgc atcgtggtgc ccgtgttcgt gcgcgtgggc
     3361 agtcgcgtgc gtcaaagtat cagttgccat ttttcagccc tgccttagta atctcatcga
     3421 aatgctggtt ttccagtttt tcgatttctg ccaccggcac gtagtaatcc acgtcctcgt
     3481 cgaagctttt cacgatccag gtgatgatca tgcctgcgaa gccaacaatc gccagccacc
     3541 agatatgcca gatcatggcg aaaccgaaga tggtggagaa agctgcaatg acgatacctg
     3601 caccgctgtt tttcggcata tgaatttctt catagtggtc aggcttttta tacgcttcgc
     3661 ctttctcttt catttcccag aatgcatcac gctcgtgaac gtgcggcaca acggcaaagt
     3721 tatagaacgg aggcggggaa gaggttgccc actccagcgt acggccaccc cacgggtcgc
     3781 cagtcaggtc acggttctgg tcgcggtcgc gaatagaaac gtacatctga ataacgaggc
     3841 agagaatacc cagcgcaatc agtactgcac cgctagctgc aatcatcagc atggtgtgga
     3901 actgcgggtc aatctgctgg ctcaaacgac gggtcatgcc catgaagccc agcgcataca
     3961 gcggcataaa ggcaacgaag aagccgatga tccagaacca gaacgcgcgt ttaccccagg
     4021 tttcattcag tttgaaaccg aaggctttag gccaccagta ggtcatccct gcgaagcagc
     4081 cgaagaccac gccgccgatg atcacgttat ggaagtgcgc aatcaggaac aggctgttat
     4141 gcagaacgaa gtccgcgccc ggtactgcca gcagcacgcc agtcatcccg cccaccgaga
     4201 aagtgacgat aaaaccgatg gtccacagca tcgcagaatg gaacacgatg cggccctgat
     4261 acatggtgaa cagccagttg aagatcttca ccccggtcgg gatggcgata atcattgtgg
     4321 tgataccaaa gaaggcgttt acgttcgcgc ccgcacccat cgtaaagaag tggtgcagcc
     4381 aaacgatgaa cgacagcacg gtgatacaga cggttgccca taccagcgag gtataaccaa
     4441 acagacgttt acgcgagaag gttgccgcaa tttcggagaa cacaccgaaa acaggcagga
     4501 tcaggatgta aacttccggg tggccccagg cccaaatcag gttgatgtac atcatcatgt
     4561 tgccacccat atcgttggta aagaaatggg tgcccagata gcgatccagg gtcaacaacg
     4621 cgacggtaac cgtcagaatt gggaaggaag caataatcag tacgttcgcg cacagtgatg
     4681 cccaggtaaa tactggcatc ttgaacatgg tcatgcccgg tgcgcgcatc ttcagaatgg
     4741 taacgaagaa gttgataccg gtaagcgtcg tacctatacc ggatagctgg agactccata
     4801 tccagtaatc aacaccgact cccggactgt actctattcc cgatagcggt ggataggcca
     4861 gccagccggt ctgcgcaaat tcgcccacgc cgagagaaac gttaaccaga atcacaccaa
     4921 caacagtaaa ccagaagctt aagttgttga ggaacgggaa cgcaacgtca cgcgcgccga
     4981 tctgcagcgg aaccaccagg ttcatcagac cgataacgaa aggcatcgcc acgaagaaga
     5041 tcataatcac gccgtgcgcg gtgaagatct gatcgtagtg gtgaggtggc aggaagcccg
     5101 cttcacccgc cgaggcaaga gcctgctggc tacgcatcat aatggcgtca gcaaaaccac
     5161 gcagcaacat cacaatcgcc acgatgatat acatgatacc gaggcgttta tggtcgacgg
     5221 aggtcagcca ctctttccac aggtaggtcc acttaccgaa gtaagtgatc aggccaacga
     5281 gcgccagacc tcccaaaata atgccagcga tcgtaaccat gacgataggt tcatggaacg
     5341 ggactgcatc aagtgataat tttccgaaca tctttattct tcctcaaccc ctttaatggg
     5401 cggattccac gtggctcatg tccatgcctt ccataccttc gtgtgcactg tgctcacctt
     5461 ctggctgggt catgtccatg ctcttaccgt gagccataaa cttgttaatc acatcggcaa
     5521 acaagtctgg tttcacgttg gagaaatatt ccacctggtt gtattcgcta ggcgcggcca
     5581 gtttttcgaa cgcagccatg tcagacatgg tgttcggcga ctgtttcgct tttgcgaccc
     5641 attggtcgaa ttcggcgcga tccggtgttg caatagcttt gaacttcatg cctgagaagc
     5701 ccggcccgct gtagctggcg gagataccgt cataagtacc gggttcgttg gcgatcagat
     5761 gcaggcgagt ctgcataccg gccatggcat aaatctggct acccagacgc ggaatgaaga
     5821 aggagttcat cacggagttg gaggtcactt tgaagtacac cggagtgttc gccgggaaag
     5881 cgatttcatt cacggtagca atgccctgtt ccgggtagat gaagaaccat ttccagtcca
     5941 tggaaaccac ttcgatggta atgggcttct cgtcgtgtgc cagcggcttg ctaggctcaa
     6001 gagcgtgagt ggttttccag gtcagtaccg caaggaagat gatgattaag ataggtaccg
     6061 tccagaccac agcttccact ttattggagt gtgaccagtt cgggctgtac ttagcatctt
     6121 tattgctcgc acgatacttc caggcgaaac caacagccat caagattgcg ggaataacga
     6181 caatcaacat caggccaaat gccgtcagta tcagtgaacg ttgctccaga ccaatctgtc
     6241 ctttgggatc taacagcgca gaattacagc cactgagcaa tacagtgcct gcaaataatg
     6301 acaaccatcc caaactttta ttgtatttcc tgagtctcat ttaacgacct caattccacg
     6361 ggatctggtg gcgtttaaag tgtgtgggca ttttacggga aggttacatt actgtaaaca
     6421 tgattaaatt cctgttactt ggatttggct gtcaggtcac atatgttgca aaacatatca
     6481 aattgatttt aagaagctgt tgcaaaaggg cgttataacg aaaggggagt gcagaaggag
     6541 tttggacg
//
LOCUS       DAJSKJ010000190         6455 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000190 DAJSKJ010000000
VERSION     DAJSKJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.194"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..348
                     /locus_tag="ON345_003416"
     CDS             <1..348
                     /locus_tag="ON345_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR3073425.1"
                     /translation="KNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQK
                     NTLLVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSE
                     PNRFYRFTPQSSH"
     gene            416..1594
                     /locus_tag="ON345_003417"
     CDS             416..1594
                     /locus_tag="ON345_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073426.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLSNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(1607..2161)
                     /gene="kptA"
                     /locus_tag="ON345_003418"
     CDS             complement(1607..2161)
                     /gene="kptA"
                     /locus_tag="ON345_003418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR3073427.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            2411..3094
                     /locus_tag="ON345_003419"
     CDS             2411..3094
                     /locus_tag="ON345_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073428.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFYGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            3091..3552
                     /locus_tag="ON345_003420"
     CDS             3091..3552
                     /locus_tag="ON345_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR3073429.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            3565..4737
                     /gene="iadA"
                     /locus_tag="ON345_003421"
     CDS             3565..4737
                     /gene="iadA"
                     /locus_tag="ON345_003421"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3073430.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            4802..5713
                     /gene="hypT"
                     /locus_tag="ON345_003422"
                     /gene_synonym="qseD"
     CDS             4802..5713
                     /gene="hypT"
                     /locus_tag="ON345_003422"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR3073431.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(5706..6098)
                     /gene="iraD"
                     /locus_tag="ON345_003423"
     CDS             complement(5706..6098)
                     /gene="iraD"
                     /locus_tag="ON345_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR3073432.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQLHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(6095..6178)
                     /gene="idlP"
                     /locus_tag="ON345_003424"
     CDS             complement(6095..6178)
                     /gene="idlP"
                     /locus_tag="ON345_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR3073433.1"
                     /translation="MENEHQYSGARHSGQAAYVAKRQECAK"
BASE COUNT         1474 a         1669 c         1628 g         1684 t
ORIGIN      
        1 aaaaacccga ttgaggtcta caaagttaac gggctattaa gtagcaatga attgcacatc
       61 agcaaagaca aggcactaca acggcaattt actttagatg atgtgtccgg cgcagaattt
      121 aaccaacaaa aaaatacgct tctggtgctg tctcatgaat ctcgcgcact ccaggaagta
      181 acgctggttg gcgaggttat cggtgaaatg tcactaacca aaggcagtcg aggtttgtcg
      241 cataacatca aacaggccga aggggttgcg atggatgcct ctggtaatat ttatatcgtt
      301 agcgaaccaa atcgttttta tcgctttacc cctcaatcat cgcattaaca ggctggcatg
      361 cttcaccttt ttgttattaa cttgttaaca acaaaaatga caaacaaggt taaacatgcc
      421 ttcgtccacg catcccgtag aacgcttttc tttcagcacc gcgcttttcg ggatgctggt
      481 tctgacctta ggtatgggtt taggccgctt cctctatacg cctatgttgc ccgtcatgat
      541 ggcggaagga tcgttttcat ttagccagct ctcgtggatt gccagcggca actatgcggg
      601 gtatctggct ggcagtctgc tattttcttt tggcgcattt caccagccat cgcgcctgcg
      661 cccatttctg ttggcttctg ccctggcgag cgggttgttg atcctcgcaa tggcatggtt
      721 gccgccattt attctggtgt tattgattcg cgtcctggcg ggtgtcgcca gcgccggtat
      781 gctgattttt ggttcgacgc tgattatgca gcacacgcgc catccttttg tgctggcagc
      841 tttgttttct ggcgttggca ttggcatcgc actgggcaat gaatatgttc tggcaggcct
      901 gcattttgac ctctcctcgc aaacgttatg gcaaggcgcc ggggcgcttt ctggcatgat
      961 gctgattgca cttacgcttt taatgccctc gaaaaaacac gccatcacac caatgccatt
     1021 ggcaaaaacg gagcaacaga taatgagctg gtggttactg gctattctgt acggcctggc
     1081 gggatttggt tatatcatcg tcgcgactta cctgccgctc atggcgaaag atgcaggctc
     1141 accattgtta accgcccatc tctggacgtt agtcggctta tcaattgtgc ctggttgctt
     1201 tggctggcta tgggcagcaa aacgttgggg ggctttgccc tgcctgacgg cgaatttgct
     1261 ggtgcaggct atctgtgtgc tgcttactct cgccagcgac tcgcctctct tgcttatcat
     1321 cagcagtctt ggttttggcg gtacctttat gggcacgaca tctctggtga tgactatcgc
     1381 ccgccaactg agcgtaccgg gaaatctcaa tcttttaggc tttgtgacgc tcatttacgg
     1441 cattgggcaa atcctcggcc cggcgctgac cagtatgctc agcaatggaa catcggcgct
     1501 tgccagcgcc actctctgcg gcgcggcggc gttgtttatt gctgcgctta tcagcacagt
     1561 gcaattattt aagctacaag tggtcacttc atgagggaag tttcccttac cactcaagaa
     1621 actcaaccgc aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggaggc
     1681 ctcgtttcgc catttcctgt gctttgacgg ttaaaataac cggcgatccg tgtcttgctc
     1741 ccactttgcg tgccgttgct tcatcagcag agagatgtac gtagtgacgc tcacctgcaa
     1801 tcagcccctg tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact
     1861 gtggcggtgt tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg
     1921 cgcggatgca cctaccatca ctggaataac taaaacgctt tttatcgctg gtggcaacca
     1981 cagtatccag caatgcacgg gttaaccgct tgcccgcttt ttgggcacaa agtatgagct
     2041 tatcgatatc cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc
     2101 ggaggacaaa acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca
     2161 tctcgttttc gtccctagat tttctgtttt tatttactga tgtcattgtc actgggttta
     2221 tgaatttact gcaactcaat ccagcgcaga accttttccc catagcgtaa tttttccgct
     2281 atgcaaaaaa cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata
     2341 aggtttttca gctccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat
     2401 ggggattgtt atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt
     2461 gggcattaaa gggtatctgg ctttttttct caccataatc tttttttacg gtgttttttc
     2521 aggaacagac agctggtggc gcgtttttga tttttccgtg ctgaacggat cgtttgggca
     2581 gttacctggt gcgaatggag ccaccacctc atttcgcggg gcgggtggcg cgggggctaa
     2641 agatggcttt ctctttgcgc tggagctggc accgtcagtc attctctctc tggggattat
     2701 ttcaattacc gatggtcttg gtggattacg cgccgcgcag cagttaatga cgcctgtgct
     2761 aaaaccgttg ttgggcattc cagggatctg ctcgctggcg ctgatcgcta acctgcaaaa
     2821 cactgatgcc gccgccggta tgaccaaaga gctggcgcag gaaggtgaaa ttaccgaacg
     2881 cgataaagtg attttcgccg catatcaaac cagcggtagc gccatcatca ccaactactt
     2941 ctcttccggc gtcgcggtgt tcgcctttct gggcacgtcc gtaatcgtac ctttagccgt
     3001 catcctggtg tttaaatttg tcggagccaa cattttgcgc gtctggctta acttcgaaga
     3061 acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg
     3121 gatatgttta tcgatggtgc gcgccgtggc tttactatcg ccacgaccaa cctgctgcca
     3181 aacgtggtga tggcgtttgt catcattcag gcgctgaaaa ttaccggcct gctcgactgg
     3241 gtggggcata tctgtgaacc cgtaatggcg ctgtggggat tacccggtga agcggcaacc
     3301 gtccttttag ccgcattaat gagtatgggt ggcgcagtcg gtgttgccgc cagtctggca
     3361 accgctggcg cattaaccgg tcacgatgtc accgttttac tgcccgcgat gtatttaatg
     3421 ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat
     3481 tacccgcata tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag
     3541 cttattgttt aacaaggagt taccatgatt gattataccg cagccggttt taccctgctg
     3601 cagggagcgc atttgtatgc gccggaagat cggggaattt gcgatgtcct cgtcgctaac
     3661 ggcaaaatta tcgccgttgc cagtaatatc ccttctgaca ttgtgccgga ctgcacggtt
     3721 gtcgatctca gcgggcagat cctctgccca ggttttattg atcaacacgt ccatttgatt
     3781 ggcggtggcg gcgaagcagg tcccacgacg cgcacgccgg aagtggcgtt aagtcgtctg
     3841 acggaagcag gcgtcacgtc agtggttggt ctgctgggca ccgactctat ctctcgccac
     3901 ccggaatccc tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg
     3961 ctgaccggcg cttatcatgt cccttcccgc actattacgg gttccgtgga aaaagacgtg
     4021 gcgattatcg atcgcgtgat tggcgtgaaa tgcgccatct ctgatcaccg ttctgccgca
     4081 ccggacgttt atcacctggc taatatggcg gcggaatccc gcgttggcgg tttgctcggc
     4141 ggtaaacctg gcgtcaccgt gttccacatg ggcgacagca aaaaggcgtt acagcctgtc
     4201 tatgacctgc tggaaaactg cgatgtgccg attagcaagt tgctgccgac ccacgttaac
     4261 cgcaacgttc cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg caccatcgat
     4321 atcaccagca gcattgacga accagtcgcc cctgccgaag gtattgcccg cgccgttcag
     4381 gcgggtattc cgctggcacg cgtcaccctc agctccgacg gcaacggtag ccagccgttc
     4441 ttcgatgacg aagggaattt aacccatatc ggtgttgccg gttttgaaac gttgctggaa
     4501 accgtgcagg tgctggtcaa agattatgat ttcagtatca gcgatgccct gcgcccgctc
     4561 accagtagcg tagccggttt ccttaacctg agcgggaaag gcgaaattct gccaggcaat
     4621 gacgcagact tactggtcat gacgccagaa ttacgcattg agcaggtata cgctcgcggc
     4681 aagctgatgg tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac ggcataaacg
     4741 gttgtcagcc gcccgaatgg acgtagaatg cccgcaaggg cggctgacag agtaaaacgt
     4801 aatggatgac tgtggtgcgg ttttgcataa tattgaaact aaatggcttt atgattttct
     4861 gaccctggaa aaatgccgca atttttccca ggcagcagtc agtcgcaacg tctcgcaacc
     4921 ggcattcagc cgccgcatcc gtgcgctgga acaggcgatt ggcgttgaat tgtttaaccg
     4981 ccaggtgacg ccgctgcaac tctcggaaca aggtaaaata ttccattcgc agatccgcca
     5041 tctgttgcaa cagttagaga gcaaccttgc agaactccgt ggtggcagcg attacgcgca
     5101 acgcaaaatt aaaatagccg ctgcacactc tctttccctc gggctgttac cgtccattat
     5161 cagccagatg ccgccgctct ttacctgggc aattgaagct attgatgtcg atgaagcggt
     5221 cgataaactg cgtgaagggc aaagtgactg tattttttcc tttcacgacg aagatttgct
     5281 ggaagcgccg tttgaccaca ttcgcttatt tgaatctcaa ttgttccccg tctgcgccag
     5341 tgacgaacac ggagaagcac tgtttaacct cgcgcagcca cactttccgt tactgaatta
     5401 cagccgcaac tcctacatgg ggcgattgat taatcgcacc ctgacgcgcc acagcgagtt
     5461 aagtttcagc accttttttg tctcttcgat gagcgagcta ttaaagcagg ttgccctcga
     5521 cggctgtggg attgcctggc tgccggaata cgctatccaa caagaaattc gcagcgggca
     5581 gctcgttgtg cttaatcggg acgaactggt gatcccgatt caggcctacg cataccggat
     5641 gaacacccgt atgaatcccg ttgccgaacg cttctggcgt gaactgcgcg aactggagat
     5701 tgtgcttagc tgacattctc cagcgtcgca ctacgccaac cgccattatc ccagaccaga
     5761 tcaaacgtta gcggttcatc gtcgtggtac agcattatct caaggctgat cgccagcgtg
     5821 tggcagtacg cttctttcaa attgacctgc aacgccgcaa tccgtggctc atgaaataac
     5881 agtgcagaac gcagctgacg gcaataccag ctctggagat gtttgtcgct ctggttatgc
     5941 agttgccact cgggcatgcc tgaaacaagt aatgacggta atgccgaatg aggagaatta
     6001 aaaagtgtga ggagatcgct gcataccgat ttacgcagca atgacgtttt attgcctgaa
     6061 atttcaggta aaacagcctg aagtgattgg cgcatcattt tgcgcactcc tgacgtttag
     6121 caacatatgc ggcttgccct gaatgccggg caccactgta ttgatgctca ttttccatag
     6181 ccattccctt ttgttcatta catccatgtc agtctgagac aggcctgaat cctgtcagca
     6241 aagtataaaa ccttacttgc ctatgaatat ctactgactt ttcaggaaaa aagtcatgat
     6301 ttgataaaaa tcatcaaact caaaaaaaaa ccgctagtat attctgaata ttccgaatag
     6361 ttacgcagat gaaaatactt gtcaccgccg ctacttaatt ccggtgggaa attatttatt
     6421 tccgcataat cagccatgta ttatttgaaa caatt
//
LOCUS       DAJSKJ010000191         6330 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000191 DAJSKJ010000000
VERSION     DAJSKJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.195"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1317
                     /gene="nrfA"
                     /locus_tag="ON345_003425"
     CDS             <1..1317
                     /gene="nrfA"
                     /locus_tag="ON345_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR3073434.1"
                     /translation="KNETFAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPF
                     SRDYNKPRGHAFAVTDVRETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGY
                     FHGKWARGGPEIVNNLGCADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAG
                     RFDQQSMVCGQCHVEYYFDGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTP
                     MLKAQHPEYETWTAGIHGKNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCAN
                     CHTQDKAALQKVVAERKQSINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDI
                     RHAQWRWDLAIASHGIHMHAPEEGLRMLGTAMDKAADARTKLARMLATKGITHEIQIP
                     DISTKEKAQQAIGLNMEQIKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            1362..1928
                     /gene="nrfB"
                     /locus_tag="ON345_003426"
     CDS             1362..1928
                     /gene="nrfB"
                     /locus_tag="ON345_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR3073435.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            1925..2596
                     /gene="nrfC"
                     /locus_tag="ON345_003427"
     CDS             1925..2596
                     /gene="nrfC"
                     /locus_tag="ON345_003427"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR3073436.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            2593..3549
                     /gene="nrfD"
                     /locus_tag="ON345_003428"
     CDS             2593..3549
                     /gene="nrfD"
                     /locus_tag="ON345_003428"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR3073437.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            3569..5287
                     /locus_tag="ON345_003429"
     CDS             3569..5287
                     /locus_tag="ON345_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR3073438.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWVGHEGSLLLW
                     VLLLSAWSTLFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            5280..5663
                     /gene="nrfF"
                     /locus_tag="ON345_003430"
     CDS             5280..5663
                     /gene="nrfF"
                     /locus_tag="ON345_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR3073439.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            5660..6256
                     /gene="nrfG"
                     /locus_tag="ON345_003431"
     CDS             5660..6256
                     /gene="nrfG"
                     /locus_tag="ON345_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR3073440.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCIGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
BASE COUNT         1341 a         1634 c         1847 g         1508 t
ORIGIN      
        1 aagaatgaaa cctttgcccc gcagcatccc gatcaatatc tctcctggaa agccacctcg
       61 gaacagtcag agcgtgttga cgccctggcg gaagatccac ggctggtgat cctgtgggcg
      121 gggtatccct tctcgcgcga ttacaacaag ccgcgtggac atgcctttgc tgtgaccgat
      181 gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg aagatggtcc gctaccgatg
      241 gcatgctgga gttgtaaaag cccggatgtg gcgcgtctga tccagaaaga cggcgaagat
      301 ggctacttcc acggaaaatg ggcgcgcggc ggcccggaaa tcgtcaacaa cttaggttgt
      361 gccgactgcc ataacaccgc ctcaccagag ttcgccaaag gcaaaccgga gttaaccctt
      421 tcccgtccgt atgcggctcg cgcgatggaa gccattggta aaccttttga gaaagccgga
      481 cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg tggagtatta cttcgacggc
      541 aaaaacaaag cggttaaatt cccgtgggat gacggcatga aagtcgaaaa tatggagcag
      601 tattacgaca aaattgcctt ctctgactgg actaactccc tgtcgaaaac gccaatgctg
      661 aaagcgcagc acccggaata tgaaacctgg acagcgggca ttcacggtaa aaacaacgtg
      721 acctgtatcg actgccatat gccaaaagtg cagaacgccg aaggcaaact ctataccgac
      781 cataaaattg gtaatccgtt tgataacttc gcccagactt gtgcgaactg ccatacccag
      841 gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc agtcgattaa cgacctgaaa
      901 atcaaggttg aagatcaact ggttcacgct cacttcgaag cgaaagcagc gctggatgca
      961 ggcgcgacgg aagctgaaat gaagccaatt caggacgata tccgtcatgc ccagtggcgc
     1021 tgggatctgg cgatcgcttc ccacggcatt catatgcacg caccggaaga aggtttacgg
     1081 atgctcggta cggcgatgga taaagcggcg gatgcacgca ccaaactggc gcgcatgctg
     1141 gcgaccaaag gcatcaccca tgaaatccag atcccggata tctcaaccaa agagaaagcc
     1201 cagcaggcca ttggcctgaa catggaacaa atcaaggccg agaagcagga cttcatcaaa
     1261 acggtgatcc cgcagtggga agaacaggca cgtaaaaacg gtctgttaag ccaataaccc
     1321 cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa tatgagcgta ttacgttcgt
     1381 tattaactgc cggggtgctg gcgtcgggcc tgttgtggag cctgaacgga attaccgcta
     1441 cccctgcggc gcaggcatct gatgatcgct acgaagttac ccagcagcgt aacccggatg
     1501 ccgcctgtct ggactgtcat aaaccagata ccgaaggtat gcatggaaaa catgcttccg
     1561 ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa ctgtcacggc cagccatcac
     1621 cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa cgagccgatg tacaaggttg
     1681 gagagcagaa cagcgtctgt atgtcctgtc atctgccgga acagttgcaa aaagcgttct
     1741 ggccgcacga tgtccacgta accaaagtgg cgtgcgccag ctgccattcc ctgcatccac
     1801 agcaagatac gatgcagacg ttaagtgaca aaggacggat taagatttgc gtcgattgcc
     1861 acagcgatca gcgcaccaat ccgaacttta acccggcgtc tgttccgttg cttaaggagc
     1921 agccatgacc tggtctcgtc gccagtttct caccggcgtc ggcgtgctgg cagccgtcag
     1981 cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc aatggggtac gttacggcat
     2041 ggtgcatgac gagtcgttat gcatcggctg taccgcctgt atggatgctt gtcgggaagt
     2101 gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt cgtagcgagc cgcagggcga
     2161 atttcctgat gtgaaatatc gcttcttccg taagtcttgc cagcactgcg atcatgcgcc
     2221 gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat gccgctagcg ggattgtcga
     2281 tgttaatcct gatctctgcg tcggttgtca gtactgcatc gccgcctgtc cgtaccgcgt
     2341 gcgctttatc catccggtca cgaagacggc ggacaaatgc gatttctgcc gtaaaaccaa
     2401 tttgcaggcc ggtaagctgc ccgcgtgcgt tgaggcttgc ccgacgaagg cgctgacgtt
     2461 tggcaatctg gacgatccta acagtgagat ttcgcaactg ctgcgccaga agcctactta
     2521 ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac cgcgtaccgt ttaaatacgg
     2581 ggaggtgagc caatgacgca gacttccgca tttcattttg aatcgctggt gtgggactgg
     2641 ccgattgcca tctacctgtt tttgattggt atttctgccg gtctggtgac gctggccgtg
     2701 ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca gtacgttgct gcgcaccacg
     2761 ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga tcctcgtctt ccacctgaca
     2821 agaccgtgga ccttctggaa gctgatgttc cactacagtt ttacctcggt gatgtcgatg
     2881 ggggtgatgc tgtttcagct ttacatggtg gtgctggtgc tgtggctggc gaaaatcttt
     2941 gaacatgatt tgcttgccct gcaacaacgc tggttgccga agctggggat cgtgcaaaag
     3001 gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa cgctgatgct ggtgttggcg
     3061 gtgctgctgg gggcttatac cggctttctg ctgtcggcgc tgaaatcgta tccgttcctc
     3121 aataacccga tcctgccggt gctgttcctc ttctccggca tctcgtccgg tgcggcggtg
     3181 gcgctgatcg ccatggcgat acgccaacgc agtaacccgc attccacgga agcgcacttt
     3241 gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct tcctgctggt ggcgtttttt
     3301 gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc tggtggcggc attaggtggc
     3361 ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc tggggctgat tgtgccaatg
     3421 ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg ccgtgctggc ggcgtgtggg
     3481 gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc tctacgccgg acagttgacg
     3541 gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc cttcctgaag tcggttttct
     3601 ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg ttgacggcct ttgcgggagt
     3661 gcggttgcgc tggcctgcca tgatgcgact cacttgcatc ggcattctgg cgcagttcgc
     3721 gctcctgctg ctcgcctttg gcgtactgac gtattgtttt ctcatcagcg atttctcggt
     3781 catttatgtc gcgcaacata gctatagcct gctgtcgtgg gaactcaagc tggcagcggt
     3841 gtgggtcggc catgaaggtt cgctgctgct ttgggtgctg ctgctttccg cctggagcac
     3901 gctgtttgcc tggcattatc ggcagcaaac cgatccgcta tttccgctga cgctagccgt
     3961 tttatctctc atgctcgccg cactgctact gtttgtagtg ctgtggtccg atcccttcgt
     4021 gcggatattt ccaccagcaa tcgaaggccg cgatctcaat ccgatgctgc aacatcccgg
     4081 tcttatcttt catccaccgc tgctttacct tggctatggc ggtttgatgg tggcggcgag
     4141 cgtggcgctg gcgagtttac tgcgcggcga gtttgatggt gcctgcgcca gaatttgttg
     4201 gcgctgggca ctacctggct ggagcgcatt aacggcgggg atcatcctcg gttcctggtg
     4261 ggcctattgc gaactgggct ggggcggctg gtggttctgg gatccggtgg aaaacgcctc
     4321 tttattaccc tggctttctg ccactgcgct gctgcacagt ttgtccctga cacgccagcg
     4381 ggagattttc cgccactggt cgctgttgct ggcgatagta actctgatgc tgtcgctact
     4441 gggtacctta attgtccgtt ctggcattct ggtttcggtt catgcgttcg cactggataa
     4501 cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt agccttgcgt ctctggctct
     4561 gtatggctgg cgagcgcggg acggtggtgc ggtggtgcgt ttttcggggt tatcgcggga
     4621 aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg ctactgatcg tgctggtggg
     4681 aacgctttat ccgatgattt acggtctgct gggctgggga cgcctctccg ttggcgcgcc
     4741 gtattttaac cgcgcgacgt taccgtttgg cctgttgatg ctggtggtga ttgtgctggc
     4801 gacgtttgtc tctggcaaac gcgtgcagct tccggcgctg gtagctcatg cgggcgtgct
     4861 gttatttgcc gctgggatcg tggtttccag cgtcagccgt caggagatca gcctgaattt
     4921 acagccgggt cagcaggtga cgctggcagg atacaccttc cgttttgagc gcctcgatct
     4981 gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg gcactgtttg accatcagca
     5041 acgcattggt gaactgatgc cggagcggcg tttttacgaa gcacgtcgtc agcaaatgat
     5101 ggaaccgtca attcgctgga acggcatcca tgactggtat gcggtcatgg gtgaaaaaac
     5161 cggagcggat cgttacgctt ttcgcttgta tgtacaaagc ggtgtgcgct ggatctgggg
     5221 gggaggattg ttgatgattg cgggcgcatt gttaagcgga tggcggggga ggaagcgcga
     5281 tgaataaagg acttctcacg ctactgctgt tatttacctg ttttgcgcgc gctcaggtcg
     5341 tagacacctg gcaattcgcc aatccgcaac aacagcaaca ggcgttaaat attgccagcc
     5401 agttacgttg tccgcagtgc cagaatcaaa acttactgga atccaacgcg ccggtggcag
     5461 tcagtatgcg ccatcaggtt tacagcatgg tggcggaggg gaagaacgaa gtcgaaatca
     5521 ttggctggat gaccgaacgc tacggagatt ttgttcgcta taacccaccg ttaacgggtc
     5581 agacgctggt gttgtgggcg ctgccagtgg tgttgttact gctgatggca ctgatcctct
     5641 ggcgagtgag ggcgaagcga tgaaagagcc caaaataccg gtgaaaatgc tgacaacact
     5701 cacgattttg atggtatttc tctgtatcgg cagttatctg ttaagtccaa aatggcaggc
     5761 ggtacatgcg gaatatcagc gtcagcgcga tccgctacat cagtttgcca gccagcaaac
     5821 cccggaagcg cagcttcagg cattgcagga taaaatccgc gctaatccac aaaacagcga
     5881 acaatgggcg ttactgggcg agtactatct gtggcaaaac gattacagca attcgctgct
     5941 ggcgtaccgt caggcgttgc aactacgtgg tgagaacgct gaactgtatg cggcgctggc
     6001 gacggtgctt tattaccagg ccagccagca tatgaccgcc cagactcgcg caatgatcga
     6061 caaagccctc gcgctggact ctaacgaaat caccgctctg atgctgctgg cttccgatgc
     6121 gtttatgcag gcgaattacg cgcaagccat cgaactatgg caaaaagtga tggatctcaa
     6181 ctcaccccgg atcaaccgga cacagctggt cgagtcgatt aatatggcga aattgttgca
     6241 gcggagatca gattaatcat ctggtgatga agattatgtt attgagttgt ataaaaaata
     6301 atcgaatgaa catatgccaa aaataatcac
//
LOCUS       DAJSKJ010000192         6229 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000192 DAJSKJ010000000
VERSION     DAJSKJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.196"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            45..236
                     /locus_tag="ON345_003432"
     CDS             45..236
                     /locus_tag="ON345_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR3073441.1"
                     /translation="MRAILLRPEVKALLRIKSDSTLTKMVNKGGFPKGFRVGLRRIGW
                     YEDEVLAWLKERERESREA"
     gene            463..906
                     /locus_tag="ON345_003433"
                     /pseudo
     CDS             463..906
                     /locus_tag="ON345_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            882..1070
                     /locus_tag="ON345_003434"
     CDS             882..1070
                     /locus_tag="ON345_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR3073442.1"
                     /translation="MATIPALAHTQNAFIWRFIIVNPSDYRVVHVVASTEREARNYCP
                     SGCVAVFAARIRQGVSHA"
     gene            1063..1248
                     /locus_tag="ON345_003435"
     CDS             1063..1248
                     /locus_tag="ON345_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073443.1"
                     /translation="MLKRFQITARAVNHQGHVIDIRQNVNATSVNAAIAILKEELIGC
                     GFTQVMIRAVVEVSTVH"
     gene            1288..1587
                     /locus_tag="ON345_003436"
     CDS             1288..1587
                     /locus_tag="ON345_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR3073444.1"
                     /translation="MKVKNSGFTASGPVRPEIHTGDIFFDKYGWMVKVISVEERRITC
                     RREGYNYDCVTPVYQFRRDFSLMKAAPRSKPTSREKERGNIQKMKSMINTFRGKK"
     gene            1584..3753
                     /locus_tag="ON345_003437"
                     /pseudo
     CDS             1584..3753
                     /locus_tag="ON345_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
     gene            3990..4415
                     /locus_tag="ON345_003438"
     CDS             3990..4415
                     /locus_tag="ON345_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3073445.1"
                     /translation="MTAQISAYGRLVADPQLKTTSKGTPMALVSMAVPIPCNQADDGQ
                     ATMWLSVLAFGRQADALAKHHKGELVSVAGNMQISQWTDQNGEMRQGWQVIADSVISA
                     RTVRPGGRKGQQGQATDALNRAKQHTGQQDDPYEDEIPF"
     gene            4451..4984
                     /locus_tag="ON345_003439"
     CDS             4451..4984
                     /locus_tag="ON345_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HCR3073446.1"
                     /translation="MTTLTISRKPKGIYGIPKKKTQTTQQDKTTSAHKVIPGNQNAPQ
                     SRQQKPTGATPWRHMTKRQGKNRRRANRLIELWPELFSLESPKPLKVGIFEDLIQDIA
                     VRAQAFGSGALRAALASYAHSPRYYRALMAGGVRYDLNSQPCGEVTQQEQQNAEARLM
                     ALKEKRKRKSNKEQAGV"
     gene            4987..5265
                     /locus_tag="ON345_003440"
     CDS             4987..5265
                     /locus_tag="ON345_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR3073447.1"
                     /translation="MIHDSKAEDLESKGLYRRAAARWAEVMQQVNTDKEREQAVKRRA
                     ECIHKAARSPVILDNFGGLREAANRTQADMGIEIAKSVWRNYQTDKVL"
BASE COUNT         1782 a         1441 c         1693 g         1313 t
ORIGIN      
        1 tttcattcaa aaagcgatat tttgctgtcc agaggaaaaa taagatgagg gctattcttt
       61 tacgaccaga ggtaaaggct ttgctacgca ttaaatctga ttcgacccta acgaagatgg
      121 ttaacaaagg cgggttcccc aaaggattcc gtgtcgggct gcgtcgcatt ggctggtatg
      181 aagatgaggt actcgcctgg ttgaaagaac gtgagaggga aagtcgtgaa gcgtaagtgt
      241 gattaacgct cgcctgatta ctatcaagta ataagacaat taagagagaa cattatgaat
      301 actggcaaaa atgcccaagc aggggcaggt tttcgttcaa ccataaaaaa tatggctgat
      361 gttattattc agttcaaacc ttacaaaaga atcaattgaa ctaatcgcac ttccgtttta
      421 aacggaaacg tacaatcgca tagcttaaac gacaaaagaa atatgctttt tacaaataaa
      481 ataaaggcga ccgcagaggg ccgccagagg atatgtatta aacttgaacg catcgccaac
      541 aatgccatat ttacagctgg tgggcaatgt gatttgtgtg gtgggcacac cttcaacaca
      601 tccgaccaca tgggccggaa aacgggatat actcgccgaa tcccttataa aacggggatc
      661 gggattacca ccccgctgaa gcaaaaggcg acacatgacg cgccagcgtc ttttttgttg
      721 tctgtacaca cgcacacctt atcaatggtg ggctgtatgg ggctgacttc ggtcaggctg
      781 gtctcctttt gcgccagttg tggtaaccct gtacagtcca ccgccagcga gcttaccacc
      841 tcctgcggtg ggtatatccc aacgcaaaag gaggctgcca aatggctact atccctgccc
      901 tcgcacatac tcagaacgcc tttatctggc gctttatcat cgttaatccg agtgattacc
      961 gcgtcgttca cgtcgtggcg tcaaccgaac gagaagcgcg taactattgc ccgtccggtt
     1021 gtgttgccgt attcgctgcc cgtattcgtc agggggtaag tcatgcttaa gcgattccag
     1081 ataaccgcac gcgctgtaaa tcatcaaggc cacgttattg atattcggca aaacgtgaac
     1141 gccaccagcg ttaatgcagc tatagcaata ttgaaagaag agttaatcgg ctgtgggttc
     1201 acgcaggtga tgatccgtgc cgtagttgaa gtgtcgactg ttcattaatc tcattaatac
     1261 agctttaaca aaaacgtaat tcgacaaatg aaagtaaaaa attctggctt tactgccagc
     1321 ggccccgttc ggcctgaaat ccacaccggc gatattttct tcgataaata cggctggatg
     1381 gtaaaggtga taagcgtgga ggaacggcgc atcacctgcc gtcgtgaagg atataactac
     1441 gactgtgtaa cgcctgtata tcagttccgg cgtgatttct ccctgatgaa ggccgcgccc
     1501 cgcagtaaac ctactagcag ggaaaaagag cggggaaata tccagaaaat gaaaagcatg
     1561 attaacacat tcaggggcaa gaaatgaaac gtgcaccgaa tttaaagcat cagccagccg
     1621 acaaaatgac ggaggttatc atttttgcgg gtagtgatgc ctgggcacat gcgaagcagt
     1681 ggcaggagat ggacgggcga cttgctggcg ataacgtgcc tcctgtctgg cttggagatc
     1741 agcaactggc agaactggaa aatctgaaca tcattgacga cgggcgctat tgtgtccggc
     1801 tgtacaaggc agggcatatc aagccatcaa acatcaatac cattgcgcaa aagctggcgg
     1861 cggcaggcat acgggatgcg aattattacc ccgacgggat gcacagccag aagcgggaga
     1921 actggcgcga atatctggaa cgtgagcgcc aaaacatcga tgacgggctg gctatccggc
     1981 ttccgacaaa agaaaacgcc accggaagtc atggcgatga tgaattaaag ccccgcgttg
     2041 aaagccgcgc agatggtgtt ttctgggtaa cacccaaagt ggacaagcag acaggcgaga
     2101 taatcagacc tgaaacgtgg ttatgttccc cgcttgaact actgggaacg gggacgatcg
     2161 gcagggagca tcaccgcgtg atgcgctgga aaaaaacggc aaatcatgaa gtcatcacga
     2221 tggcggttcc gtgcggtggc attggcgacc gtgacggctg gcggttgttg aaagaccacg
     2281 gccttaacgt gacaaccaac ggcaaataca gagcaatact ggctgactgg atgcagttaa
     2341 gcggaaacca tgaggaatgg cagttaagca caacaacggg gtggcatttt ggcgcgtaca
     2401 tcatgccgga cggttcagtc attggtgagt ctgaaaaacc gatcctgttt accggaaaaa
     2461 cggctgccgt taatggttat tccgtagcag gaacgacgga gggctggcac gatacagtgg
     2521 cgcggttggc tggtggcaat ccgtccatga tgctgggggt ggcggtatcg ttatcagcgc
     2581 cattaatcgg gctggtgggc gctgacggtt tcggtgtgca tcttttcgaa caatcatcgg
     2641 caggtaagac caccacgcag aacatcgcat ccagtttgtg gggagagccg gacgcacaac
     2701 ggctgacctg gtacggaacg gcgttaggta tcgccaacga ggccgaagca cacaatgacg
     2761 ggctgttacc cctggatgaa ataggccaag ccggaaacgc acgggaggtg tccacgtcgg
     2821 cttatacgct gtttaacggt tcgggaaaat tacagggggc gaaggacggc ggcaacaggg
     2881 agataaaaca ctggcgaaca gtggcaatca gtaccggaga aatggacgtt gaaacattcc
     2941 tcaaaagtga ggggataaaa gtcaaagcgg ggcagcttgt ccggttgctt aacgtgccga
     3001 tggagaagtc cacgcagttt cacgaataca gcacaggaaa ggcgcacgca gtttcacgaa
     3061 tacagcacag gaaaggcgca cgcagacgcg ttaaaggatg cctggacagc aaatcacggg
     3121 gcggcaggtc gtgagtggat taaatggcta gcagcccacc agcagaaggc taaggacacg
     3181 gtaagggcat gccgtgaacg gtggcgcaac ctgataccgg agagctacgg cgaacaggtt
     3241 caccgtgtag gggaacgatt cgccatactg gaggccgcgc ttgtgctgtc aggcaatgtt
     3301 acgggctggg atgttcaggc gtgccgtgat gccattcagc ataactttaa tgcctgggtg
     3361 aaggagttcg gcacgggtaa caaggaacac aggcagatta ttgaacaggc agaggcgttt
     3421 cttgctgcct acggcattag caggtttgcg cctgtcaatt atgaccctgc aagcctgccg
     3481 atttcagaac tgtacggcta cagggaaagc gacgggcgtt acggtgagtc tgttttgttt
     3541 tacgtcctgc cggagccatt taaatcgcat gtggcgaagg gatttaacaa ggatgccgta
     3601 gcaagagtcc ttcacgaagc tggaatgctg aaaaagccag ccagcgggga aggatggcaa
     3661 atcagaacac cacgccttaa acacatgaag ggggcacgac taagggtgta cggcctttta
     3721 cttgcacagg atcatgatga tgaaggaggt taatctctcg cgtgtaggtt taaaaacact
     3781 tgtcccacct gtcccaacct gtgggaaaca cacataacac actgaattat attgggtaac
     3841 atcagaaaaa gtgggacaag tgtgggacaa gttaaggtga ttttgggaca agttaggagc
     3901 attttgggac aagtcagcag gcgttatttt ttgcctggct ggtggacatt aaaaatccaa
     3961 ccaacattta aaaccggaga tcagacaata tgacggcaca aatatcagct tacgggcggc
     4021 tggtagctga cccgcagtta aagaccacca gcaagggaac accaatggcg ctggtgagta
     4081 tggctgttcc cattccgtgc aatcaggcag atgacggaca ggcgacgatg tggttatccg
     4141 tcctggcatt tggcagacag gccgacgcac tggcaaaaca ccacaaaggc gaactggtga
     4201 gcgtggcggg taacatgcag ataagccagt ggacagacca gaacggggaa atgcggcaag
     4261 gctggcaggt catcgcagac agcgtaatca gtgcgcgaac cgtgcgacca ggaggcagga
     4321 aaggacagca agggcaggct actgacgcac tgaacagggc aaagcagcat acgggccagc
     4381 aagatgaccc gtatgaggac gaaataccat tttaactgcc agtggattca ctaacagagg
     4441 ttaacaaata atgactacac tgactattag cagaaaaccg aaaggtattt acggcatacc
     4501 aaagaaaaag acacagacga cacagcagga taaaaccaca tcagcgcata aagtgatacc
     4561 cggcaatcag aacgcaccgc agagccgcca acagaagccc acaggggcga caccatggcg
     4621 gcatatgacc aaacgccagg gcaaaaaccg caggcgcgct aaccgtctta ttgaactatg
     4681 gcctgaatta ttcagcctgg aatcaccgaa gccgcttaag gtagggatat ttgaagacct
     4741 gatacaggat attgccgtca gggcgcaggc ttttgggtca ggggcattac gtgcagcgct
     4801 agcatcttat gctcattctc cgcgttatta ccgtgcctta atggctggtg gcgtgcgtta
     4861 cgatctgaat agccagccat gcggcgaggt gacacaacag gaacagcaga acgcggaagc
     4921 acgactgatg gcactgaaag agaagcgcaa acgtaagtcg aacaaagagc aagctggcgt
     4981 atgagtatga ttcacgacag taaagcggaa gatctggagt ctaaaggcct gtaccggaga
     5041 gcggcggccc ggtgggctga ggtcatgcaa caggtgaaca ccgataagga gcgcgaacag
     5101 gcagtaaaac gtcgcgcgga atgtatccac aaggcagcac gttcgcccgt catactggat
     5161 aatttcgggg gcttgagaga ggcagcaaac cgcacacagg cggatatggg gatagaaatc
     5221 gcaaaatctg tctggcggaa ctaccagaca gacaaggttt tatagtggca gttctgcatt
     5281 attttttgtg ttattgagtt gtagagcgtt tcaggagttt tttgaatcac atgaacgttt
     5341 cgctggtgga tattatcgac aagttgcagc caggccgacg cgtgccagct cgccgcagtg
     5401 cctggcatct tccgtacatc gtctttctcg aacggcgcgg aaacgatttg attgcgttga
     5461 atcccgtcac agaggatagc gttacgctat ctcctgagag tctactagcc acagactggg
     5521 agtactgcta aacacaaacg catcgagggt gctggcgtgt tgccagcccc accccctccg
     5581 aaatgatccc ccaaaaagtg aaaaatgatc ccccattaac gaacagaaaa caaacgaacc
     5641 aagagcaata aataagcata gtttgcaatt cttgacaaaa ccatccccta tttataaccc
     5701 taccaaattt aaaataacac atacaaaaca ataagttaca aaatcaccaa cggatcattg
     5761 cggaaaaaac ggttagtgcc tttttcgaaa agggatcatt ttgttgctta aactcatatg
     5821 cggattacat atcaatacac tagtgatagc cgttcttgaa tgccacagaa acgcgctgta
     5881 atcgattctg gcgcaattag ctgtgctagg taatgattta cctgtctggg gatgttgcgc
     5941 acgtctggat tgcgttacca ccgaagattg tgcgcgtatg gtggctgtta cagtaccctc
     6001 ccgacaacgc aaaattgcgg cagcacagtc ggtttaccgc gctaacacgg cttgattcac
     6061 ttgctcgact tgtcccactt ccccaccaac ttgtcccaaa acagcctaga cttgtcccac
     6121 cttacctaat tcatttctaa caaaatcaat ctattaagtg ctagtgggac aggtgggaca
     6181 ggtgggacag gtagttttgt atatatatat acgaaaattg cattcagga
//
LOCUS       DAJSKJ010000193         6178 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000193 DAJSKJ010000000
VERSION     DAJSKJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.197"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            32..961
                     /gene="pfkB"
                     /locus_tag="ON345_003441"
                     /pseudo
     CDS             32..961
                     /gene="pfkB"
                     /locus_tag="ON345_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
     gene            1062..1352
                     /gene="ghoS"
                     /locus_tag="ON345_003442"
     CDS             1062..1352
                     /gene="ghoS"
                     /locus_tag="ON345_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR3073448.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1458..2318
                     /locus_tag="ON345_003443"
     CDS             1458..2318
                     /locus_tag="ON345_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR3073449.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRHWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSSNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2359..2895)
                     /locus_tag="ON345_003444"
     CDS             complement(2359..2895)
                     /locus_tag="ON345_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR3073450.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3042..3710
                     /gene="hxpB"
                     /locus_tag="ON345_003445"
     CDS             3042..3710
                     /gene="hxpB"
                     /locus_tag="ON345_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR3073451.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVAPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3873..4463
                     /locus_tag="ON345_003446"
     CDS             3873..4463
                     /locus_tag="ON345_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3073452.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4596..5987
                     /gene="tcyP"
                     /locus_tag="ON345_003447"
     CDS             4596..5987
                     /gene="tcyP"
                     /locus_tag="ON345_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR3073453.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(5991..>6178)
                     /locus_tag="ON345_003448"
     CDS             complement(5991..>6178)
                     /locus_tag="ON345_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073454.1"
                     /translation="RLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAME
                     HLKSKATAPIQNGPLPA"
BASE COUNT         1509 a         1524 c         1559 g         1586 t
ORIGIN      
        1 ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt tgacacttgc
       61 gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa aactgcgctg
      121 taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg ccattgccca
      181 tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg aacacctggt
      241 ttcactgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag actggacccg
      301 gcagaattta catgtacatg tggaagcaag cggtgagcag tatcgttttg ttatgccagg
      361 cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg aaattgaatc
      421 cggggccatc ctggtcataa gctgaagcct gccgccaggt gtgaagctgg aaaaattaac
      481 ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc atcatcgaca gttctggcga
      541 agcgttaagt gcagcactgg cgattggtaa catcgagttg gttaagccta accaaaaaga
      601 actcagtgcg ctggtgaatc gcgaactcac ccagccagac gatgtccgca aagccgcgca
      661 ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc cacaaggagc
      721 gctgggtgtt gatagtgaaa actgcattca ggtggtgcca ccaccggtga aaagccagag
      781 taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg cagaaaatgc
      841 ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca cactcaatca
      901 gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc tttcccgcta
      961 acaaaaacat tcccccagca ttgggggaat catcaccaac ctgtcggcaa cgcgtttctc
     1021 cgactatgct caaaagtcat gtgataacaa aggggtgaac tatggccagt ggcgatcttg
     1081 tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt aacgagttga
     1141 ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag ggaaatatcc
     1201 atgagctggg aactaacact tttggactta tcagtaccca aagtgaagaa gaaattagag
     1261 aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatt accatcacga
     1321 cctgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa tcagtctgtg
     1381 gtgtgcgtta gctcgtgttt ttacaccgta ttcttgcgct aaccttatga tctggcagac
     1441 aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag cagttaggtg
     1501 aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc gcatggcatt
     1561 tgcgctatgc aggacgtgac tttttcgtca aatgtgatga aagggaactg cttcccggtt
     1621 ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc accgtgccta
     1681 aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat tatctcccac
     1741 ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg cgtttacatc
     1801 aatggagtga ccaaccacaa tttggcctcg attttgataa cgcgctctcc acaactccac
     1861 agcccaacac ctggcaacgt cactggtcaa cgttttttgc tgaacaacgg attggctggc
     1921 agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc atcgtcgagc
     1981 atattcagca gcgtctggcc tcgcatcaac cgcagccttc tctgttgcac ggcgatttat
     2041 ggtccagcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct gcctgctact
     2101 ggggtgaccg agagtgcgac ctggcgatgt taccgctgca tactgaacag ccgccacaaa
     2161 tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa cgtcaaccgg
     2221 tttaccaact ctacacgctg ttaaatcgtg caagattatt tggcggtcag catttggtta
     2281 ttgctcagca gtcattggat agattattag cagcatgata tgggttgagg ataatggccg
     2341 ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat aacctgcagc
     2401 aatacaaata accggcagga tatacagtga gaaaaactgc aggaaaatag tatggtgcgg
     2461 aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt tggctttttc
     2521 cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac tcatccgggc
     2581 accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc agaacataac
     2641 gttcacgcca ccattaaaat taggggtcgg ggagttatac cagaacagat tcaaaaaagg
     2701 cgtgttcgcc tgcatcatat cgatcatgac atgagtaaag tcgagcatga ccgcattaat
     2761 gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca atgtagagat
     2821 caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa tgcgtccagc
     2881 ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta gtttacctgt
     2941 agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat cataagcacg
     3001 gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc ccgcgtcaga
     3061 ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct ttatgggatc
     3121 gagccgaact ggatgtgatg gcaagcctgg gggtggatat ctcccgtcgt aacgagctgc
     3181 cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc cggcaaccgt
     3241 ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc atttcactgg
     3301 ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc tgttgcgtta tgcaaagaac
     3361 aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa aaagtgttga
     3421 ccatgtttga cttacgcgac agtttcgatg ccctcgcctc ggctgaaaaa ctgccttaca
     3481 gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt gccccactga
     3541 cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc ttctaaagca gcccgcatgc
     3601 gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta ttagcaaacg
     3661 tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa tgaccagggg
     3721 cagtgatcac gctgcccctg gttctttatc tgaatttgca ttcaactgac ggattaatcg
     3781 tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa aaatcctata
     3841 ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac cttctctttt
     3901 ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg gcacagggtg
     3961 actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac atcgatcacc
     4021 caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa accgatcgcc cgcgcttttg
     4081 ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca accttttacc
     4141 ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct acaaggaatg gtgctgggtt
     4201 atttgagcca catacttgcc gatatgctga cacccgccgg tgttcccctg ctctggccat
     4261 gccgctggcg tttccgcttg cctatcctgg ttccccaaaa gggcaaccaa ctggaacgtt
     4321 ttatctgcat ggcattattt gtctggtcgg tatggatgcc ccattcatta cccgagaaca
     4381 gcgctgttcg ttggtcatcg caaatgatca ataccttgca gatccagttt catcggctta
     4441 ttaagcatca ggttgaatac taaaaagaca aaaatcacct ttctggaata agcaattcca
     4501 tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta tgactgctac
     4561 gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta attgcgaaca
     4621 tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccataaa cagtggagtc
     4681 tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt gccctgcata
     4741 ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt aacatcgttg
     4801 gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc gcctctattc
     4861 tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt tttctgacca
     4921 tcggtacgct tttgtttacc acgctgattg cggcgctggt cggtgtgctg gtcaccaacc
     4981 tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca cgtctgaacg
     5041 ccattgaaag taactatgtt ggtaaagtct ctgatctgag cgttccgcag ctggtcttgt
     5101 cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg tcaattatca
     5161 gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg aaggatgatg
     5221 cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct acaaagctgg gtgatgaaac
     5281 tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg accaaagtgg
     5341 ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc gcgtcctacc
     5401 tcggtctgct gattatgttt gcagtgcatg gcattctgct gggcattaat ggcgtgagtc
     5461 cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc agccgttcca
     5521 gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc gttcctgaat
     5581 ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc tgcgccggtt
     5641 tgtatccggc aatgctggcg gtgatggttg cgcctacggt tggcattaac ccgctggacc
     5701 cgatgtggat tgcgacgctg gtcggtattg ttaccgttag ttccgcaggc gttgccggtg
     5761 tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc tgcgatgggc ctgccagtaa
     5821 ccctggtggc gctgttaatc tccgttgaac cgcttatcga catgggccgt acggcgctaa
     5881 acgttagtgg ctcgatgaca gctggcacgc tgaccagcca gtggctgaag caaaccgata
     5941 aagccattct ggatagcgaa gacgacgccg aactggcaca ccgttaattc ttatgctggc
     6001 aacggtccgt tttgtatagg ggccgttgct ttacttttta aatgttccat cgcggcgatg
     6061 catactgact catatttaaa cagatcgtca gcgaaaaaaa tgtccaactt atcaatacat
     6121 tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa cctcataccc gagacgtt
//
LOCUS       DAJSKJ010000194         6149 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000194 DAJSKJ010000000
VERSION     DAJSKJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.198"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..366
                     /locus_tag="ON345_003449"
     CDS             <1..366
                     /locus_tag="ON345_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR3073455.1"
                     /translation="TWQGFVYVAIIDVFAGYIVGWRVSSSMETIFVLDALEQALWTRR
                     PSGTIHHSYKGSQYVLLAYTQRLKEAGLLASTGSTGDSYDNTMAESINGLYKAEVIHS
                     KSWKNGSQSRMARSAFRGS"
     gene            855..1871
                     /locus_tag="ON345_003450"
     CDS             855..1871
                     /locus_tag="ON345_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003837718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3073456.1"
                     /translation="MKRTVRPTISDVAKAAKTGKTSISRYLNGEKHLLSEALLARIEN
                     AIADLDYRPSLMARSLKRGRTRLIGLIIADITNPYSVDVLSGIEAACRDKGFTPLVCN
                     TNNEVNQEQHYLDLLRSYQVEGIVVNAVGMREEGLNRLQQSALPMVLIDRKIPDFACD
                     VVGLDNTQAATTATEHLIEQGFEAILFLSEPLGTVNTRRERLNAFHATLTRYPGVVAQ
                     NAEVPLAENTLLENTLRQFHQQHRGMRKAVMSANGALTLQVARALKRIGLNWGSDIGL
                     LGFDELEWAELAGVGITTLKQPTWQIGYAAVEQVIRRIEGMAEPVREHVFSGELIIRG
                     STAR"
     gene            1971..2720
                     /locus_tag="ON345_003451"
     CDS             1971..2720
                     /locus_tag="ON345_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3073457.1"
                     /translation="MQRKIIVVTAAYGYEHVQAAGGQKALLPVIAKSGADGVEIRREL
                     FTYTDLLALVELGLAIEHAGLQACYSAPEPLFTENGYLNPQLPLLLQEAKDLKSLWLK
                     VSLGHFCRPDQLDTLRDWLASSGMALVVENDQTPCGRLQPMLHFNEICQQIGLPVKLT
                     FDMGNWLWVGDSPEEAAHQLAHSVGYIHVKAALPHKESFRAVSPENTDNRWLKLLTML
                     PADVPRGIEFPLEGADLAAVTRRYVDLLRKE"
     gene            2725..3687
                     /locus_tag="ON345_003452"
     CDS             2725..3687
                     /locus_tag="ON345_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3073458.1"
                     /translation="MKHSLDVITIGEAMAMFVATQTGDLAEVEKFITRVAGAELNVAT
                     GLARLGLKVAWVSRIGNDSFGRMVLNTLQKEGIDARGVSCDNDHATGFQLKSKVENGT
                     DPIVEYFRKGSAASYLSLDDYNERWFASARHLHLSGVAAALSASSYTLLDHAARTMKA
                     LGKTISFDPNLRPVLWNSEKEMIEKLNHLAFQADWVLPGLKEGTVLTGQQTPEGIADF
                     YLQHGVKAVVLKMGADGAWYKTVDGEKGSVAPVKVENVVDTVGAGDGFACGVISALLE
                     GRTLHQAVTRGNKIGALAIQVQGDSEGLPTREQLGESLPDKNES"
     gene            3758..5038
                     /locus_tag="ON345_003453"
     CDS             3758..5038
                     /locus_tag="ON345_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073459.1"
                     /translation="MNSSTNATKRWWYIMPIVFITYSLAYLDRANFSFASAAGINEDL
                     GITKGISSLLGALFFLGYFFFQIPGAVYAERRSVRKLIFVCLILWGGCASLTGMVSNI
                     PALAAIRFILGVVEAAVMPAMLIYISNWFTKSERSRANTFLILGNPVTVLWMSVVSGY
                     LIQAFGWREMFIIEGVPAIIWAFCWWVLVKDKPAQAGWLSESEKAALQTQLEQEQQGI
                     KAVRNYSEAFRSRNVILLCMQYFTWSIGVYGFVLWLPSIIRSGGENLGMVEVGWLSSV
                     PYLAATVAMIIVSWASDKLQNRKLFVWPSLLIAAIAFIGSWAVGANNFWLSYTLLVIA
                     GAAMYAPYGPFFAIIPEMLPRNVAGGAMALINSMGALGSFLGSWFVGYLNGVTGNPSA
                     SFIFMGVALFVSVWLTFIVKPATHQPLPVGVRHA"
     gene            5060..6049
                     /gene="ghrB"
                     /locus_tag="ON345_003454"
     CDS             5060..6049
                     /gene="ghrB"
                     /locus_tag="ON345_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR3073460.1"
                     /translation="MKPSIILYKKLPDDLLQRLEKHFTVTQVSNLSPQTVEQHAEAFA
                     NAEGLLGSSEKVDEALLEKMPKLRATSTISVGYDNFDVDALNAREILLMHTPTVLTET
                     VADTLMALILATSRRVVEVAERVKAGEWTASIGPDWFGSDVHHKTLGIVGMGRIGMAL
                     AQRAHLGFNMPILYNARRHHKEAEERFNARYCNLDTLLQESDFVCLILPLTEETHHLF
                     GAEQFARMKSSAIFINAGRGPVVDENALIAALQKGKIHAAGLDVFEREPLPVDSPLLS
                     MANVVALPHIGSATHETRYGMAACAVDNLINALQGKAIINCVNSYIISSRISK"
BASE COUNT         1544 a         1417 c         1602 g         1586 t
ORIGIN      
        1 acatggcagg gctttgttta tgtggccatt attgatgtat tcgccggata catcgtggga
       61 tggcgggtct catcatccat ggaaacgata ttcgtgctgg atgcactgga gcaggcgtta
      121 tggacccgta gaccgtctgg caccatccat cacagctata aaggctctca gtatgtattg
      181 ctggcctaca cgcagcggct taaggaagcc ggattactgg catcaacagg gagcacaggc
      241 gactcgtatg acaacacgat ggcggagagc atcaatggtc tttacaaggc ggaggtaata
      301 cacagtaaga gctggaaaaa cggctcccaa agtcgaatgg caaggagcgc attccgggga
      361 tcctaacagt tgccgatcga attgctcaga gagcgataaa acatttcatg gaagaaaatt
      421 ttgactcaat ttcccatacc gatttttatg gttaccgact aggtaaatca gctcatgacg
      481 ctatgaaact atgtgccatc tggtgctggc attatagctg tattctggag gtgggtatca
      541 gtgctttttc gaccatgtca gatatgagct ggtgctcaaa gtacagggac accgcggagt
      601 gcctgaattg ggcatcctgt atcgcctatg cttgattgat gcgacaatgc tgagttgcga
      661 aaatggagag cgaataactc gaacgcgata tactcgtcag ggtgggggat tagtatccta
      721 cttgctaact tgtacattga ttatttatgt gatttgtttg tgaaaataga atatcgggga
      781 tgccgtttga gaggcataca gatgatattg tctgcccatt gttcacgaat gaataaaaat
      841 atttcaggaa aaatatgaaa cgaaccgtgc gcccaaccat tagtgatgtt gcaaaagccg
      901 caaaaaccgg caaaaccagc atctctcgtt atctcaacgg agaaaaacat ctgctttccg
      961 aagcattgct ggcacggata gaaaacgcaa ttgccgatct tgattaccgc cccagcctga
     1021 tggcccgaag cctgaaacgt ggccgtaccc ggctgattgg cctgattatc gccgatatta
     1081 ctaatcccta ctctgttgat gtattaagtg gcattgaggc ggcttgtcgc gataaaggat
     1141 tcactcctct ggtgtgtaat accaataacg aggtgaatca ggaacaacac taccttgatt
     1201 tactacgcag ctaccaggtg gaaggtattg tggtcaatgc cgtaggaatg cgtgaagaag
     1261 ggctaaatcg tctgcaacag tcagcactgc caatggtgct tattgatcgc aagatccccg
     1321 attttgcctg tgatgttgtt gggctggaca acacgcaagc cgcaactacc gctaccgaac
     1381 acttaattga gcaaggattt gaagccatac tctttctcag cgaaccgctg ggcacggtaa
     1441 acacgcgacg tgaaaggcta aatgcttttc acgccactct gacgcgttat ccgggtgttg
     1501 tggcgcagaa cgcggaagtt ccactggctg agaatacgct tctggagaac acgcttcgcc
     1561 agttccacca gcaacatcgg ggaatgcgca aagccgtgat gtcagcaaac ggcgcactca
     1621 cgctacaggt cgcccgtgca ctcaaacgta ttggcctgaa ttggggcagt gatattggcc
     1681 tgctcggttt tgatgagctg gaatgggcag agctggctgg cgtggggatt actacgctca
     1741 aacaaccgac ctggcaaatt ggttatgcgg ctgtggaaca ggtgatacgc cgtattgaag
     1801 gtatggcaga acctgttcgt gagcacgttt tctccggcga gttgattatt cgcggatcaa
     1861 cagcacgtta atttccttcg tgatagcgat cataatttca aaactctgta cttttctttg
     1921 gaaccgattc caattagcgt tatagcaatc acgctagtca gggagttccc atgcagagaa
     1981 aaattatcgt ggtcaccgcc gcttacggtt atgagcacgt tcaggcagca ggtggacaga
     2041 aagcacttct gccggtgatt gcaaaatctg gtgccgatgg cgttgaaatt cgtcgtgagc
     2101 tgtttactta tacggattta ctggcactgg tggaactggg cctcgccatt gaacatgccg
     2161 ggcttcaggc ctgttattcc gctcccgaac cgttatttac agaaaatggt tatcttaatc
     2221 cgcaactgcc tctgttgctg caggaagcga aggatctgaa atctctgtgg ctgaaagtct
     2281 ctttaggaca tttttgtcgc cctgatcaac tcgatactct acgtgactgg cttgccagca
     2341 gtggtatggc attagtggtt gaaaacgatc aaaccccctg cggacgctta cagcctatgc
     2401 tgcatttcaa tgaaatttgt cagcaaattg ggctgccagt caaactgacc tttgatatgg
     2461 gtaactggtt atgggtaggt gattcaccgg aagaggcggc gcatcagtta gcccattccg
     2521 tgggctatat ccatgtaaaa gcggctcttc ctcacaaaga aagttttcgt gccgtctccc
     2581 cggaaaacac cgacaatcgc tggctgaaat tactcacgat gcttcctgcc gatgtgccac
     2641 gtggtatcga atttcccctg gaaggggctg atttggcggc agtaacacgc cgttatgtcg
     2701 atttattacg taaggagtga agagatgaaa cactctctgg atgtcatcac tatcggtgaa
     2761 gcaatggcga tgtttgtggc tacgcaaaca ggcgatttag ctgaagtaga aaaatttatc
     2821 acgcgcgtgg caggtgcaga gctaaatgta gcaactggcc tggcacgtct gggcttaaag
     2881 gtcgcctggg taagccgcat cggaaatgac agcttcggtc gcatggttct caatacgttg
     2941 caaaaggagg ggatagatgc ccgtggcgtc agttgcgata acgaccacgc aaccggcttt
     3001 cagcttaaat ctaaggtgga aaatggaacc gatcccattg tggaatattt ccgtaaaggc
     3061 tcagccgcca gttacctctc actggacgac tataacgaga gatggtttgc cagcgcacgt
     3121 catttacacc tgagcggcgt tgctgctgcc ctgtctgcaa gctcttatac cttactggat
     3181 cacgctgcgc gcacgatgaa agcactgggt aagactattt cgttcgatcc caatttgcgt
     3241 ccggtactgt ggaatagtga aaaggagatg attgaaaaac taaaccacct cgcttttcag
     3301 gctgactggg tacttccggg gttgaaagaa ggcactgtct taaccggtca acagacaccg
     3361 gaaggtatag ccgatttcta tcttcagcat ggcgtcaaag cggttgtgct gaaaatgggg
     3421 gctgatggcg cctggtataa aacagtcgat ggtgagaaag gcagcgtcgc cccggttaaa
     3481 gtcgaaaacg tggtcgatac cgtcggggcc ggagatggct tcgcatgcgg tgttatcagt
     3541 gcactattag aaggtcgcac gctacatcag gcagtcactc gggggaataa aattggcgca
     3601 ctggcaattc aggtacaggg cgacagtgaa gggcttccga cacgtgaaca actgggagag
     3661 tcactccccg ataagaatga gtcataaaac aatctgatac gtactcctct gtccctactt
     3721 acagaggcgc tctaatctga agcatagagg caagcctatg aacagttcaa caaacgcaac
     3781 aaaacgttgg tggtacatca tgcctatcgt gtttatcacg tatagcctgg cgtaccttga
     3841 tcgtgcgaac tttagcttcg catccgccgc gggcatcaac gaggatcttg gcatcaccaa
     3901 aggaatttca tcgctgctgg gggccctttt cttcctcgga tacttctttt tccagatccc
     3961 aggtgcggta tatgcagaac ggcgtagtgt gcgcaaactg atttttgtct gtctgatctt
     4021 atggggtggt tgtgcctcat tgacaggtat ggtcagtaat attccggcac tggcagcaat
     4081 tcgctttatt ctcggggtgg tagaagctgc tgtcatgcct gccatgctga tttatatcag
     4141 caactggttt accaaatctg aacgctcacg tgccaatacc tttctgatcc ttggtaaccc
     4201 ggtgacggtg ctgtggatgt cggttgtctc cggctatttg attcaggcgt tcggctggcg
     4261 cgaaatgttt atcatcgaag gtgttcccgc cattatctgg gccttttgtt ggtgggtgct
     4321 ggtaaaagat aaacctgcgc aggcaggctg gctttcggaa tcagagaaag cagcgttgca
     4381 gacacagtta gagcaggaac aacaaggcat taaagctgtt cgcaactata gcgaagcctt
     4441 ccgctctcgt aacgttatct tactgtgtat gcaatatttt acctggagca tcggggtata
     4501 tggatttgtg ctctggttgc catcgattat ccgcagcggt ggcgaaaacc tgggtatggt
     4561 tgaagtcggc tggctgtctt cagttcccta tctggcagca acagtggcaa tgattatcgt
     4621 ctcctgggca tcggacaaat tacaaaatcg taagctcttt gtctggccgt cactgctgat
     4681 tgccgcgatt gcctttatcg gctcctgggc cgtgggtgct aacaatttct ggctttctta
     4741 taccttactg gtgatcgcag gcgctgcaat gtatgccccg tatggtcctt tctttgctat
     4801 tattccagaa atgctgccac gtaatgttgc cggtggcgca atggcattaa tcaacagcat
     4861 gggtgcgctg ggctcgttcc tcggctcatg gtttgttggc tatctcaatg gggtaaccgg
     4921 taatccatca gcatcattca ttttcatggg ggtagcgctt ttcgtttcgg tatggctaac
     4981 atttattgta aaaccagcaa cccaccaacc gctacccgtt ggcgtgcgcc acgcgtagac
     5041 attaatcacg gagattcgca tgaagccgtc cattattctt tataaaaagt tacctgacga
     5101 tttactgcaa cgccttgaga agcactttac cgtcacgcag gtaagcaacc taagcccaca
     5161 aacggtagag caacacgcag aggcgtttgc aaatgcggaa ggtttgttgg gttcaagcga
     5221 aaaagtggat gaggcattac tggaaaaaat gccaaagcta cgcgcaacgt caacgatctc
     5281 ggtaggctat gacaattttg atgtcgatgc gcttaatgcg cgagaaatct tgctgatgca
     5341 tacgcctacc gtattgacgg aaaccgtcgc ggatacgttg atggcactca ttctcgccac
     5401 ttcccgtcgg gtggtggaag tcgcagaacg cgtaaaagca ggtgaatgga cagccagcat
     5461 tgggcctgat tggtttggta gcgatgtgca tcacaagact ttgggcattg ttggtatggg
     5521 gcgtattggc atggcgctgg cgcagcgagc gcatctgggc ttcaatatgc cgatcctcta
     5581 taacgcccgt cgccaccata aagaagcgga agaacgcttt aacgcccgct attgcaatct
     5641 ggatactctg ctacaagaat cggattttgt ctgcctgatc ctaccgctaa cggaagaaac
     5701 tcatcacctg tttggtgctg aacaattcgc caggatgaaa tcctccgcca ttttcattaa
     5761 tgccggacgt ggtccggtgg tagacgaaaa tgcactgatt gcagcattgc agaaagggaa
     5821 aattcacgcc gctgggctgg atgtcttcga acgagagcca ctgccagttg attcgccgtt
     5881 gctctcaatg gcaaacgtcg tcgcattacc gcatattggt tctgccaccc atgaaacgcg
     5941 ttatggcatg gcggcatgtg ccgtagataa tttgataaat gctctacagg gtaaagcgat
     6001 aattaactgt gtaaattctt atattatttc ttcgagaata tcaaaatgat aaaagactta
     6061 attaaattaa cgcgttctta ttacttaagg atatgataca ggtgtatccg aaaaatgaac
     6121 caccccgggt ttcctagaga gtgttttat
//
LOCUS       DAJSKJ010000195         6114 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000195 DAJSKJ010000000
VERSION     DAJSKJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.199"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..654)
                     /locus_tag="ON345_003455"
     CDS             complement(<1..654)
                     /locus_tag="ON345_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073461.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     "
     gene            complement(644..1507)
                     /locus_tag="ON345_003456"
     CDS             complement(644..1507)
                     /locus_tag="ON345_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR3073462.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(1547..2152)
                     /locus_tag="ON345_003457"
     CDS             complement(1547..2152)
                     /locus_tag="ON345_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCR3073463.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            2410..2907
                     /locus_tag="ON345_003458"
     CDS             2410..2907
                     /locus_tag="ON345_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR3073464.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            2999..3931
                     /locus_tag="ON345_003459"
     CDS             2999..3931
                     /locus_tag="ON345_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3073465.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(4065..5942)
                     /gene="ehaA"
                     /locus_tag="ON345_003460"
                     /pseudo
     CDS             complement(4065..5942)
                     /gene="ehaA"
                     /locus_tag="ON345_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
BASE COUNT         1528 a         1542 c         1361 g         1683 t
ORIGIN      
        1 ttgtgaaaca ccgccaccaa attcatgaat gcggacgctc agctcctggc tgccggtgcc
       61 ggatgcgcca acaataccga tattgccgcg acgcacggcg ttaccaaaac agagcgccgc
      121 gccgttaata atcgccgtgc cgcagtccgg ccccatcatc agcagccctt tttcgtgggc
      181 caactgcttg agcgccagtt catcttcaac tgagacgtta tcggaaaaca gcatcacgtt
      241 gagatcgttt tgcagcgcct gacgcgcttc gcgagcggca aacagaccgt tgaccgaaat
      301 caccgccagg ttactttccg ggatatgctt tttggcgctg gcaatagtgg cgtaacgcgc
      361 ctcgtgcgag ccgctttgcg ctttggtgtt gaacagttct tcaatctcca ccagcaactg
      421 ctcgttgtcc gcacccgatt taccattgat gacaatcatc aggtcgccgt ttttcgcctg
      481 ttccagctcc ggcgtcagca gtcctaaatt tttcagtaca cctttgttca tttcggtcgc
      541 catcgccaca aatgcctgct cgacgccgtc gagtttattt gcacgcgtgg agatagacat
      601 cagcgagaca gaatcaaaat aggtattcgg tttaatgact attttaactg acataattcc
      661 ctccgtaggt ttggctcagc gcacaaccat cggccatgcc atacagcatg tcgcagccgg
      721 aactggagcc aatatgttta attttttcta ttgcctgagt tgcgttccca gggatgtcat
      781 aaataatgtt gtgaataaaa cgatgaatat tttcccggca acgttgttgt aatgcggctt
      841 ccagcgttat agcacttaat aatgtggtgt tatttctgcc gcgctgcata ccgagataaa
      901 attcctcttt gtatttttcc gacggatgcc cgggaataaa taaaataagc gccagacctg
      961 ttaaataatc gtcggctgat ggcgtaaggc caatccctaa cccaatcata ctggcgaccg
     1021 ctgcggcgat attttgtttt tcgtttactg cctgaataac tgcccgtcgt ttaatatgta
     1081 gttctttatt taacgcctga taaaacggat tatcgccgcg ataattaaac agggtttcat
     1141 tttcccccag gtgctgatga ataatgtcgt gccactgtag ccaggcgata aggtgaaaat
     1201 tcacagcggt catttgcaag gttggggaca gccaacgccg acaacgactc atctctatat
     1261 gaagatgttg accaaccgta ataccttgat cgtcaaactg aaccttatca ccatgccgga
     1321 acagatcatc aaagtgagtg agtgccaacc gacaactgtt tggcgcattg tcgtactctt
     1381 cacaaagcag cgtcagcaac tgctggcgag ccgggagtgc gatatttacc gctctggcaa
     1441 agacctgttc aacctgtcct tgcccccgct gcgccagaaa atccgcatcc gcagttagcg
     1501 cccacacaca ccgtctttct ggtgtgttga tagcaagcct cccggtttat gcacctgcgg
     1561 caatcagtaa ctgcgcaatc tcttcaaagc cccgttcccg cgccagttcc agtggcgttt
     1621 tgccatattt atcggtcaga tgcgggctgg caccgtgttc cagcaataac tgcacaatcg
     1681 cctgctgttt aataccacca tcattaagca caatcgcttc cagtagcggc gtccagccta
     1741 catggttggt ctggttaacg ttaatctccg tatgcgccaa aagctctttt acaatactta
     1801 aatggccttt ttcacaggca ggcgtcaggc cgacaccgcc aaaacgggtt acgcaattaa
     1861 gatcgggttt agccggtaaa ataattcgta gtagcgttaa atcatcgttc agacagctaa
     1921 ttaaaaaagg atttaaacag gtatgatctt gcttattaat atccgctccg gcatcaatta
     1981 atgcctgaac gcaagcatat tgctgatata aacttgccag cgtaattgcc gttttcccct
     2041 gacgatcgca ggtattaata tcgacaccca gcgcaaggca ggtttttact ttatcgatat
     2101 caccctgttg tgcagccaat aaatagtcgg caggtagatt ttttatactc attcacattc
     2161 ccctggtttt tgtggctgtt ttcgatgaat tgagaatagc aacgataatc tgataaaaga
     2221 acccgcttaa atttaattcg ccgtatctcg cctttttgtt gaataatatt caacaatctt
     2281 ttcgcagtgt gcggtagtgc aatatttgtt tctttgaccc gatacccttt cactcaatca
     2341 ggttttattt ctctatagtc tgcacatgta agagtagctt tcgtccatgt ctaaccagag
     2401 gggaacaaga tgaacggact cacggcaacg ggtgtcaccg tcggtatttg tgcagggctt
     2461 tggcagttag tgtcttcaca tgtcgggctc tctcaaggat gggaattatt aggaaccatt
     2521 ggcttcgtcg ccttttgcag tttttatgcc gcaggcggcg gtaaatcggg gtttatcaga
     2581 agtctggcgg ttaattattc cgggatggtg tgggcgtttt tcgccgcgct gactgctggt
     2641 tggctggcct ctgtaagtgg actgtccgcg ttctgggcga gcgtaattac taccgtaccc
     2701 ttctcggcgg tagtggtctg gcaagggcgt ttctggttac tgtcctttat tcccggtggc
     2761 tttctcggca tgacgctgtt ttttgccagc gggatgaact ggacggtgac gttactgggt
     2821 tttctggcgg gtaactgcgt ggggataatt tcagaatatg gtggacaaaa attaagcgaa
     2881 gcgacgacta aacgcgacgg gtattaaaag ttattcaaaa atgattatct atctttaacc
     2941 gatacctcta cagtcatagc tggagaaatt catagtagat aatggcgaga atggtttaat
     3001 gaactcaatt tttaccgaag agaatctgtt ggcatttact accgctgcgc gttttggcag
     3061 cttcagtaaa gccgcagaag agttgggttt aaccacttcc gccattagct acaccatcaa
     3121 gcgtatggag acggggctgg atgtggtgct gttcactcgc agtacccgca gcattgagtt
     3181 aacggagtcc gggcgttatt ttttccgcaa agctaccgac ctgctgaatg atttttatgc
     3241 catcaaacgc agtatcgata ctatttcgca gggcatagaa gcgcgtgtgc gcatttgtat
     3301 taatcagctg ctttatacgc ccaaacacac cgcacgcttg ttacaggtgc tgaaaaagca
     3361 gtttcctacc tgccagatta ccgtcaccac cgaagtgtat aacggcgtct gggatgcgat
     3421 tattaataat caggccaata tcgccatcgg cgcacccgat acactactgg acggcggcgg
     3481 tattgattac actgaaattg gcgcgattcg ctgggctttt gctatcgccc cggatcaccc
     3541 gttagccttt gtgccggaac ccatcgccga aagccaactg cgtctctacc ctaatattat
     3601 ggtggaggac accgcgcata cgattaataa aaaagtgggc tggttgctgc acgggcagga
     3661 gtcaattctg gtgccagatt tcaacaccaa atgtcagtgc cagattttag gtgaaggaat
     3721 tggttttttg ccggattaca tggtccgtga ggcgatgacg caatccctgc tggttacccg
     3781 acaaatccat aatccgcgcc aggattcgcg catgttactg gcgactcagc attctgctac
     3841 gggtcaggtg acgcagtgga ttaaaaaaca atttgcgcca aatggcatat taacggggat
     3901 ctatcaggat ttactgcatc gggagaatta atcgctgcca acctcccgat gcgctgtgaa
     3961 tagcggttta catcaaccac cagctttcat tacccattca gagataaatt tattacccgg
     4021 tactggcgga gagtacaaat agccctgcaa aaagtgaacg cctttacccc actcgtcaga
     4081 cgatagtcgt aagtgccgac ggccgctttt tgccctttct gctgaatatc acgttgggca
     4141 ctgctggaga ggatttgccc atttttatcc tgcaattgca gattgcccgc atcgcccaga
     4201 acttccgcac cttgcgcatc aaccaactta atggtgctgt taccatcatc gacttcagtg
     4261 agcgataacg cagagtcaat atcacgagat actgagctta caacgtcact gtcagaaacc
     4321 tgaatcacac cctcgccgcg caaatccagc gttttactaa cgttgaccgt cccttctgtc
     4381 atctgtgcac ctgctgtcag ggcaccaaaa tcaacggttc cgccggagaa tcccaaagtg
     4441 ctaatggagt gagcgccctt acccactgtt accaggcttc cctgacccgc agtgatcccg
     4501 cctctctgca gaaactcagc attcttttcc aggttaaatg tcgtgttctg gaacgccagt
     4561 gaaccctgga accagtctgt ataagcaaac tcatcgaagg agaattcgtg attccccatg
     4621 tttatttcaa caatacctct accttgaaga tcgttatcaa atacccaatc tttattggca
     4681 ttgatttgca gtttgccatc aacatccagg tccgcacggc cgatgttctt gtcttcagaa
     4741 ataaccgcga cagcaccaga agcgacatca atatatccgc tgaatgcata gttattgccg
     4801 gtcagcgcca ctgtcccact gtcgatactt attgtaccgt tgccggaaat cttattggca
     4861 aaggtcatgc tatcgtcagc cttcaggtgt aatttcccgc ggttgttgat ttctgcggta
     4921 cctatatttt gggtgatccc taaagcgcca ttttgctcaa ttttgacttt accgctaaag
     4981 gcggagttat ccccggttaa gtaagtcgta ccattgagcg cattgatata gcccaggccc
     5041 tcgctgtcac tgacaatatc cgcggaaata gtcgcgtttt tatccgtgtg gtttaaggtg
     5101 attttcttct cggatgtctt tgcccacaca aactcaattt tttcggcatc aataatccct
     5161 gcagccttcg ctgcgcttcc ttcctgtgca cctaacgcta actcagagtt ggtgcttaaa
     5221 ctaatagtcg gcgcagagat tttaccacct tccgaaacag caagtgttgt atcggagttt
     5281 tgatcataat aacccaggct aattttctca ctggcattaa cttcaccgcc gttattaact
     5341 gacaggtact gattaccacc gtaactgccg taaacgccca taaagaaggt tttagaattt
     5401 acctggctgc cttcaccact gacatttccc tgcaaatagg tcagacggct ggtgccctta
     5461 acatggattt tgccgccgtt ggagacatta aaatagccgc gatcgtgtct catgcctgag
     5521 tttgactcat acaaatcatc agcatataac tctctgccag cattggagac attcaggaac
     5581 cccgtacctt tcagcaaaaa gttaagtggg ccattgatag ttacctttcc tccggtaata
     5641 tccaacccac caccagaagc gagagtcaga cgcggacggg aagtatctgt cgcattgatt
     5701 accagattgg cattgtcagt caccgtaata cggtaatcga tgctctggtt ctctttgcca
     5761 aaatcaatag ttttatccta gtttacgatg acatcatcgg cgaataccag agatgaatat
     5821 aaacttatcg cgctaattaa aacagcccgg gacttttttc cttttactct gctccccagc
     5881 tccgagcaga cgctccaaca gttacgggac ctgttccact ttagccgata tattttattc
     5941 atcacaatct ccagcaatac atccaaataa aaccaagatt gcataaacag caacgcatta
     6001 aatgccattc tggttactgt tcatcgcata taaaaattca aaaattttgc atagcattgg
     6061 caaccacata tctgagtatg tagttcagag ggtaataaaa tagcaagtta ctac
//
LOCUS       DAJSKJ010000196         6082 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000196 DAJSKJ010000000
VERSION     DAJSKJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.200"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(7..465)
                     /locus_tag="ON345_003461"
     CDS             complement(7..465)
                     /locus_tag="ON345_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR3073466.1"
                     /translation="MTITDLVLILFIAALLAFAIFDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(520..1371)
                     /gene="manZ"
                     /locus_tag="ON345_003462"
     CDS             complement(520..1371)
                     /gene="manZ"
                     /locus_tag="ON345_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR3073467.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1384..2184)
                     /gene="manY"
                     /locus_tag="ON345_003463"
     CDS             complement(1384..2184)
                     /gene="manY"
                     /locus_tag="ON345_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR3073468.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2247..3218)
                     /gene="manX"
                     /locus_tag="ON345_003464"
     CDS             complement(2247..3218)
                     /gene="manX"
                     /locus_tag="ON345_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR3073469.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFNNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3681..5237
                     /gene="yoaE"
                     /locus_tag="ON345_003465"
     CDS             3681..5237
                     /gene="yoaE"
                     /locus_tag="ON345_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR3073470.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(5241..>6082)
                     /locus_tag="ON345_003466"
     CDS             complement(5241..>6082)
                     /locus_tag="ON345_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR3073471.1"
                     /translation="LVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQC
                     IGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHV
                     GINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVK
                     LAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIEL
                     VAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIM
                     PLR"
BASE COUNT         1506 a         1536 c         1537 g         1503 t
ORIGIN      
        1 cataacttat tgagttgaaa cgagaagctt ataaattttt tccagatcgt cgatatttcg
       61 aacgcggatt aacagccgac gctgttctaa ttgcatcacc aacacgccat cttccgacaa
      121 gttcatcgct ttgattcggc tatattcaat ccagacattg gcgaagaaaa aacctttttg
      181 tttaaagatg atcttcggaa cgcggatcca gaatatataa aaacccatca gagccagtgc
      241 acttaataac catgtcgtta ttagcgcgcc atgattcgtg acgttgttat aaataagaat
      301 gacaatcaat ccgacgaaga taacgctatc gatgcgacca cgccggagca aaggaattgc
      361 caacagggtg gggccgttgc ggcggggcat gatgaactga tcgaagatcg cgaaggccag
      421 gagtgcggcg atgaaaagaa tcagtaccag gtccgtgatt gtcattaatc ctccagataa
      481 aaaaacgggg ccaaaaggcc ccggtagtgt acaacagtct tacagtccca gcaggccgca
      541 agcgtaacca gcgataccga tgacgaagaa gccaacgatg atccacagcg ggttaacttt
      601 tttgcgcagc agccacatac aagcaaaggt cagcagcagt ggtaccaggc ctggcattaa
      661 ctggtccaga atagtctgga cagtggtaac gtgttctttg cccgtctggt cagtaatgcg
      721 agagacaacc agcgggatgt tgacatgtgt ccacttgtta accaatgccc ccatgacaaa
      781 caggccgagg atagacgccc cttccgtcag tttttgcagg aagccaccac ccatatcttt
      841 aacgatatcg atacctttgg agtaaccata cgctacgccg tagtaacggg ttgccagacg
      901 caccaggtta aacaggatga agaacagcag cggacctaac aggctgccgc tcatcgcgat
      961 accggcaccc agtgctgcaa ataccggacg tacggttccc cagaagatcg ggtcgcctac
     1021 accagccagc ggtcccatca aaccgacttt gataccgttg atagcaccgt catcgatctc
     1081 tgcgccatta gcacgctgtt cttccagcgc cagggttacg ccgagaatcg gcgcagccac
     1141 gaacggctgg gtattaaaga actccaggtg acggcgaata gcttgtttac gagcttcgtt
     1201 gttctcaggg tagaggcgac gaattgccgg taccatagag aagcagaaac ccagtgcctg
     1261 catacgttcg aagttccatg aaccctggaa gaggttagaa cgcaggaata cgccacgaat
     1321 atcactttga gtgagttttt tctcggtggt agtttgagtt gtatcaacca tttcgctcac
     1381 ctgttagtcc agttcgttat cgagatcgtt gttaccagct gcctgagcag gcgcaccggc
     1441 tacgcggttg tatttcgggc taagttggat gtagagcact gccataacag taccgatcac
     1501 acccagagca accaggttaa agttggtgaa tgctgcggtt acgaagccga ggtagaagaa
     1561 cggcatcagg tagccagcac gcatcatgtt gataaccatc gcataaccaa ccaccacgat
     1621 catgccaccg gcgatattca gaccattggt caccacttcc ggaatcgcat tcagcatgtt
     1681 ctgtacttcg ctggtaccaa cagacagcgc aacgatgacg gccggaatag ccacacgcat
     1741 tgcttgcagg aacagagaag aaacgtggat ccaggaaatc gctgtcaggt tgccgttatc
     1801 agcagcctta tccgcagcgt gctggaaagc aacggtaata gtacgaacga tgatggtcag
     1861 tacctggccc gcagcggcca gagggattgc cagtgcgata cctgcaccaa tgctctgatg
     1921 acctgcgata accagaatgg tagaaatgat agaagccaga gcggcgtcag gcgcaactgc
     1981 agcaccgatg ttcatccagc ccagcgcgat catttccagc gtaccaccga taataatacc
     2041 ggttttcata tccccaagaa cgatacccac cagggtacac gcgattagcg gacggtgaaa
     2101 ctgaaattca tcgaggattg atcccatacc tgcgatacag gctacgataa ataccagcac
     2161 aatttgaaga gtggtaatct ccattgtact tctcctgttt acgtaagact aaagtgtgaa
     2221 aactgagtga taatcaacac aatacgttac ttatcgattt tgctgatcag atccatcatt
     2281 ttcagtttcg gatcggtgga aaccttacgg acttccagct caataccgcg cgcattcagt
     2341 ttcttgaacg cctcgatatc tttttcatca accgaaaccg cgttattcac ctgggtttta
     2401 ccctgacgga atgccatacc accgacgtta acagaggtga ttttcacgcc gccttcaacg
     2461 agacgctcta catctgttgg gttgttaaat aacagcatta cgcgttcgcc agcatatttc
     2521 gggttgttgt agacgcgaat cattttggca acatcaacta cgtgtgctgt tacgcccgga
     2581 ggtgcaacct gggtgagcag tgtcttacga acggtatccg cagccacttc atcactaaca
     2641 acaataatac gggagacatt ggtttctttg gtccagcggg tggcgacctg accgtgaatc
     2701 agacggtcgt cgatacgcgc aaggccaata accatgtagt cgtttggccc cattggtttt
     2761 gccggagttg gagccgcttt tggtgctgcg gcagcgggtg ctggcgcggc tttttcaacc
     2821 ggtttggctt tcagtgcttt cacgccttca cggcctgttt ctactgccag cgcaaccagt
     2881 tcatcaaagc ttgggtcatc atcacgagcc attaacgttt ccacgagcat tggaatgtta
     2941 acgcctgcaa tgacttcata atgctctttg tcgacgacaa tgcggctggc agcattgaac
     3001 gggctgcctc cccatgtatc aacgagaaac agcacgcctt tagtggtgtc gagttttgcc
     3061 aactgagcgt tgtacttttc aatcagcgtt tcggcatttt cacctggaac gaaatcgatc
     3121 cagccgacgt tttcctgctc gcctaacagc atttctgccg ttttaagcaa ctgctctgca
     3181 gcccaaccat gtgtgcctat aacaatagca atggtcactt gctacctcct ttattatcgt
     3241 taacacctca acgtgccaga tgtatttttg aatcgtcgtc cacaatcgaa tcgattcaga
     3301 taagggctac aaccaaaaac ccctgcattt cgcgatttat tttagatatc gaaaaaatta
     3361 ttttatgtga tgaagatccg taatttaact ttcgattagc agaaatttcg aaaggtaaaa
     3421 tatccttgtc acattcgttt gcaaaggaag gtaaatcttt gccaaatcag aggcgtctct
     3481 gatatgttta actcccgttt aacaaccatg gagtataggg cagtagcccg cagtatggat
     3541 cgtcaccgac gtcatttcag catcaggcct tttaacgcct acctttctgg cactctatgc
     3601 cgcacctttc gtttgcattt tgtcgttacg cctgcattat ttctggcgtc gaatagctat
     3661 tccttaagca ggagcttgtc atggaattct taatggaccc ctcaatttgg gcggggctac
     3721 tcacgcttgt tgttctcgaa attgtgctgg gtatcgataa cctggtcttc atcgccattc
     3781 ttgctgacaa actgccgcca aaacaacgcg ataaagcgcg tttgctgggg ttatcactgg
     3841 cgctgattat gcgtctgggg ctgctgtcgc tgatttcatg gatggtcacg ctgaccaaac
     3901 cgctatttac cgtcatggat ttctccttct ccggacgcga cctgattatg ttgttcgggg
     3961 ggatattctt gctgttcaaa gcaacaaccg aactgcatga acggctggaa aaccgcgatc
     4021 atgattccgg ccacggtaaa ggctacgcca gtttctgggt ggtcgtcaca cagattgtca
     4081 tccttgacgc cgtcttctcg ttggatgcgg taattactgc agtagggatg gttaaccatc
     4141 tgccggtgat gatggcggcg gtagtgattg cgatggcggt tatgttgctg gcatccaaac
     4201 cgctgacgcg attcgttaac cagcacccca cggtggtggt gctctgtctg agcttcctgt
     4261 taatgattgg tctgagtctg gtggcagaag gtttcggttt ccacattccg aaaggttacc
     4321 tgtatgccgc gattggcttc tcgatcatca tcgaagtgtt taaccagatt gcgcgtcgca
     4381 actttattcg ccaccagtcg actttgccgc tgcgagcgcg tactgccgat gccatcctgc
     4441 gtttgatggg cgggaaacgt caggccaatg ttcagcacga tgccgataac ccgatgccga
     4501 tgccgatccc ggaaggtgca tttgccgaag aagaacgtta catgattaac ggcgtactga
     4561 cgctggcgtc gcgttctctg cgcgggatca tgacgccgcg cggtgaaata agctgggttg
     4621 acgctaatct cggggtcgat gaaatccgcg agcaactgct ctcttcaccg cacagtctgt
     4681 tcccggtatg tcgcggtgaa ctggatgaaa tcatcggtat tgtacgtgct aaagaactgc
     4741 tggtggcgct ggaagagggc gttgatgtgg cggcgattgc ttcggcgtct ccggcgatta
     4801 tcgtcccgga aaccctcgat ccgatcaacc tgttgggcgt gctgcgtcgt gctcgcggga
     4861 gctttgttat cgtgaccaac gagtttggtg tggtacaagg tctggtcacg ccgctggatg
     4921 tgctggaagc cattgcgggt gaattcccgg acgctgacga aacgccggaa atcattactg
     4981 atggtgacgg ctggctggta aaaggcggta cagatttgca tgccttgcag caggcgcttg
     5041 atgttgagca ccttgccgat gacgatgata tcgcgacggt cgcgggcctc gtgatctcgg
     5101 caaatggtca cattccccgt gtgggcgatg tgattgatgt agggccactg catatcacca
     5161 tcattgaagc caatgattat cgtgttgatc tggttcgcat tgttaaagag caaccggcgc
     5221 acgatgaaga tgagtaagca ttaacgtaac ggcataatgg gcgtgatatg tccattatgc
     5281 cgggcgggcg gcggttggct gcccgccagc catttgggaa aatcacgtag cggcatcggc
     5341 tgtgcgtaca aatacccttg caaaatatgc acgccatgac ggcgcaaata cttcgcctgt
     5401 tcttgcgttt ccacgccctc cgccaccagt tcaatattca gtctttgccc cagcgcgatg
     5461 atgatatcgg tcaccgtcga gttaaccgcg tcagaaccta tagctgcggt aaatgactta
     5521 tcaattttca gcacgtcagg acgtaatgtt tcaagccagg aaaatgaact gttgccggtg
     5581 ccgaagtcat caatcgccag tttgacgttt ttacgatgta gctcgcgggc aatccgataa
     5641 tcaacatcca gtaaggcatc gcgttcggtg atttcgagga tcagttgctg aatcgggtga
     5701 gcgctaaacc agtactgatt gagatctttt atcagcacac cacggcgaaa atggctgggt
     5761 gcgacgttaa tgccaacatg aaactgacta ctcatcggga aaacatggcg ctgacgaatg
     5821 gtttctgcca tcacatagcg ggtcagtggc acaattaaat gatgttcttc cgcgatagga
     5881 ataaacacat ccggtgaaat ccagccctga cgcggattgt tccagcgcag caaaatctct
     5941 acaccaatac attgctggct gcgcgcatta agcaaaggct gacagaacaa ttcgaactca
     6001 tgttgcgcca ggcccagatt gatttcgcgg gaaaagctca tccggtaagc ggtcgccagc
     6061 caggcgatgt agcccaccag ta
//
LOCUS       DAJSKJ010000197         6071 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000197 DAJSKJ010000000
VERSION     DAJSKJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.201"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(105..1775)
                     /gene="betA"
                     /locus_tag="ON345_003467"
     CDS             complement(105..1775)
                     /gene="betA"
                     /locus_tag="ON345_003467"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCR3073472.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDIGDNDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLMHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1789..3261)
                     /gene="betB"
                     /locus_tag="ON345_003468"
     CDS             complement(1789..3261)
                     /gene="betB"
                     /locus_tag="ON345_003468"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCR3073473.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3275..3862)
                     /gene="betI"
                     /locus_tag="ON345_003469"
     CDS             complement(3275..3862)
                     /gene="betI"
                     /locus_tag="ON345_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HCR3073474.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3991..6024
                     /gene="betT"
                     /locus_tag="ON345_003470"
     CDS             3991..6024
                     /gene="betT"
                     /locus_tag="ON345_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR3073475.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1215 a         1693 c         1698 g         1465 t
ORIGIN      
        1 atgaagcatg agagttacct cagtgtttat ataaggattc ggtccccctc tctggaacgg
       61 taactctcaa tctgatcggt tcctgcgtta gttcacatca cgactcattt tttcgctctc
      121 accggcatcc catttgccac aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca
      181 cgaatcatat ccgctatttt ctcgccaatc ataattgtcg tggcgttcaa attcccggtg
      241 ataatctgcg gcataatcga cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg
      301 ccttcgccgt caaccacgga catctcgtcg taacccattt tgcaggtacc gcacggatgg
      361 aaggcggttt cggcgtggtt acgcacgaac tcatcgagct gttcatccgt ctgacattcc
      421 gtgccggggc tgatttcgcg gccacgatac tgatccagcg cgggttgatg catgatctcg
      481 cgggtgatgc gaattgcgtc gcggaactcc tgccagtcct gctcgtgcga catgtagtta
      541 aacagaatcg ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg cccacggctt
      601 ggcgagcgca ttgagccgac gtggcactgg aaaccgtgct ctttcactgc attcgagccg
      661 ttatagttaa tcgctactgg caggaaatgg tactgaatat tcggccacgc aaattcctca
      721 cggctgcgaa taaatccacc tgcttcaaag tggttgctgg caccaacgcc agtgccgcca
      781 aacagccact ccgcaccgat tttcggctgg ttccaccact gcagggcagg gtagagggaa
      841 accggttctt tgcactcata ttgcagatac atctccagat gatcctgaag attttcgccg
      901 acgccgggta attcatgcat cagcggaata tcaaactccg ccagcagttc agcgttgccg
      961 acgccggagc gttgcaggat ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct
     1021 ttgttggccg ttgcgcgggt tgggatggtg ctgtcgcctt ccagccactc gacgccaacc
     1081 gcgcgtttgc cgtcaaaaat gatgtgatcg gtcatggcgt gagtacgaat ggtcaggtta
     1141 gggcgcgatt tggcctgatc gagataaccg cgcgcggtac tggcgcgacg gccctgcggc
     1201 gtgacggtgc gatccatcgg gccaaaacct tcctgctgat agccgttgag atcgtccgtg
     1261 cgcgggtagc ctgcctgcac gcccgcttca atcatcgctt caaacagcgg attgacgccg
     1321 ggtttggagg tggtgacgct caccgggcca tcaccgccgt gatagtcgtt atcaccaata
     1381 tcgcgcgtct cggccttgcg atagtagggc aggcaatcga gatagctcca gttctccagg
     1441 ccgggttctt tcgcccagtt gtcgagatcc agcgcattgc cacggatgta gcacatgccg
     1501 ttgatcagcg acgatccacc caggccttta ccgcgtccgc actccatgcg gcggttattc
     1561 ataaacggtt caggctctgt ttcataggcc cagttgtagc gtttgccctg tagtgggaac
     1621 gccagcgctg cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca
     1681 agcagcagca cggaggtatt cggatcttca gtcagacggg tagcgagaac gttgccggct
     1741 gagccggcac caataatgat gtagtcaaat tgcaaataaa cctcctggtt agaatatgga
     1801 ctggaattta gccatctcaa cctggatgga cttcacctgg gtgtaactct ggagcgtcat
     1861 cacgccgttc tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca tctctgccgg
     1921 ggattcgccc caggtgttga tccagcaaat acccgcttcc agctgatgaa tgacgcgatg
     1981 cgcgcggttc aggtccgccg tcacgatacc cgccgccagg ccgtagtcgg tatcattggc
     2041 gcggcgaatc acttcgtcct cagattcata agtgagaagc gacatcaccg gaccgaagat
     2101 ctcttcgcgc acgatggtca tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca
     2161 tgcgccgtta tcgaagccat cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc
     2221 ttcctctttg cctttggcga tatagcgcag cacgttatcg cgatgcggga agctgaccag
     2281 cgggccgaag ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc
     2341 cagaattttc tgctcaaatg cggctttgca tttcgccgga acgaagacgc gggtgccatt
     2401 ggtacacacc tgaccggagc tgaagaagtt tgccatcatg gcgatatcgg cggcgagatc
     2461 gagatccgca tcatcgaaaa cgatcagcgg tgatttaccg cccagttcca tggtcacttc
     2521 tttcagggaa gaggccgccg agttagccat cacttttttg ccgctggcga caccgccggt
     2581 aaatgacact ttggcaatgc ccggatgctc ggtcaaatat tgcccggtct ccgcgcccac
     2641 ccccggcaac acgttaaata cgccgtccgg caggcccgct tcgctgtaaa tttcagccag
     2701 ctttaacgcg gtaagcgggg tgacttcgct cggtttgaaa atcattgcgt tgcctgccgc
     2761 cagcgccggg gcggatttcc acagggcaat ctgaatcggg tagttccatg cgccaatccc
     2821 cgccaccaca cccagcggtt cgcggcgggt ataaacaaat gacgtctcac gcaacgggat
     2881 ctggctgcct tccagcgccg ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt
     2941 aacgatatcg acggttgagg tttccgaata tgcttttccg gtatcgaggg tttccagttt
     3001 tgcgagttcg tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg
     3061 ctccatagcg gtcatcgccg cccagatttt ttgcccctgt tgggcactct tcacggcgcg
     3121 atcgacatcc tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt tggccgggtt
     3181 aatggtctcg aaggtgcgac cgctggtggc ggaggtataa ccaccatgta tataaagctg
     3241 ctgttctgcc attcgggaca tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa
     3301 taaagtgacg ggtcagcgag tgtgcgagag gtttatccag cggtttgccg cttagtgccg
     3361 cgcgcagcca taatccgtca atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt
     3421 cgcgcggcaa ttcgcggcga aactcgctca ccagattcga cagcaagcgg cggctactga
     3481 cctgctgtaa acgatagagc atcggctgat gcatactgct ggcccagaac gccagccagg
     3541 ctttcatcgc cgcactgctc acctgcgttt catcgaagtt tccgccaaca atcgcctgta
     3601 atcgctgctc tgcactgccc tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca
     3661 gctgactggt gatatcgcgc atggttgctt ccagcagacc atttttatcc ctgaaatagt
     3721 ggctgatgat ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt
     3781 gcatgcccac ttcatttatt gcttccagcg tggcgtcgat cagttgtctg cgccggatcg
     3841 actgcatccc caatttgggc attttcgcca ctccattcat cagcgttgtt tatctattaa
     3901 agcggttatt gattggacgt tcaatataaa atgtgtctta attgttacga atttgatttt
     3961 aaatggtaac aataacagtg gggatactgg atgacagacc tttcacacag cagggaaaag
     4021 gacaaaatca atccggtggt gttttacacc tccgccggac tgattttgtt gttttccctg
     4081 acaacgatcc tgtttcgcga cttctcggcc ctgtggattg gccgcacgct ggactgggtt
     4141 tctaaaacct tcggttggta ctatctactg gcggcaacgc tctatattgt ctttgtggtc
     4201 tgtatcgctt gttcgcgttt tggttcggtg aagctcgggc cagaacaatc caaaccggaa
     4261 ttcagcctgc tgagttgggc ggcgatgctg tttgctgccg ggatcggtat cgatctgatg
     4321 ttcttttccg tagccgaacc ggtaacgcag tatatgcagc cgccggaagg cgcgggacag
     4381 acgattgagg ccgcgcgtca ggcgatggtc tggacgctgt ttcactacgg cttaaccggc
     4441 tggtcgatgt atgcgctgat gggcatggcg ctcggatact ttagctatcg ttataatttg
     4501 ccgctcacca tccgctcggc gctgtacccg atcttcggta aacggattaa cggacctata
     4561 ggtcactcag tggatattgc agcggtgatc ggcactatct tcggtattgc cactacgctc
     4621 ggtatcggtg tggtgcagct taactatggc ttgagcgtac tgtttgatat tcccgattcg
     4681 atggcggcaa aagcggcact gatcgccttg tcggtgataa tcgccacgat ctctgtcacc
     4741 tccggtgtcg ataagggcat tcgcgtgtta tcggagctta atgtcgcgct ggcgctggga
     4801 ttgatcctgt tcgtattgtt tatgggcgac acttcgttcc tgcttaatgc gctggtgctg
     4861 aatgttggcg actatgtgaa tcgctttatg ggcatgacgc tcaacagttt tgccttcgac
     4921 cgtccagttg agtggatgaa taactggacg ctcttcttct gggcatggtg ggtggcatgg
     4981 tcgccgtttg tcggcttgtt ccttgcgcgt atctcgcgtg ggcgtaccat tcgccagttc
     5041 gtgctgggca cgttgattat tccgtttacc ttcacgctgt tatggctctc ggtgttcggc
     5101 aatagcgcgc tgtatgaaat tatccacggc ggcgcggcat ttgccgagga agcgatggtc
     5161 catcctgagc gcggcttcta cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc
     5221 tccgtcgcca ccattactgg cctgctgttt tatgtgacct cggcggactc cggggcgctg
     5281 gtgctgggga atttcacctc gcagcttaaa gatatcaaca gcgacgcccc cggctggctg
     5341 cgcgtcttct ggtcggtggc gattggcctg ctgacgctcg gcatgctgat gaccaacggg
     5401 atatccgcgc tgcaaaacac cacggtgatt atggggctgc cgttcagctt tgtgatcttc
     5461 ttcgtgatgg cggggttgta taaatctctg aaggtagaag attaccgccg tgaaagtgcg
     5521 aaccgcgata ccgcgccgcg accgctgggg cttcaggatc gcctgagctg gaaaaaacgt
     5581 ctctcgcgcc tgatgaatta tccgggcacg cgttacacta aacagatgat ggagacggtc
     5641 tgttacccgg caatggaaga agtggcgcag gagctgcggt tgcgcggcgc gtacgtggag
     5701 ctaaaaagcc tgccgccgga agaggggcaa cagttggggc atctggattt gttggtgcat
     5761 atgggcgaag agcaaaactt tgtctatcag atttggccgc agcaatattc ggtgccgggc
     5821 tttacctacc gcgcacgtag cggtaaatcg acctactacc ggctggaaac cttcctgtta
     5881 gaaggcagcc agggcaacga cctgatggac tacagcaaag agcaggtgat caccgatatt
     5941 cttgaccagt acgagcggca ccttaacttt attcatctcc atcgtgaagc gccgggccat
     6001 agcgtgatgt tcccggacgc gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg
     6061 gcgcgggaat c
//
LOCUS       DAJSKJ010000198         6009 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000198 DAJSKJ010000000
VERSION     DAJSKJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.202"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(81..452)
                     /locus_tag="ON345_003471"
                     /pseudo
     CDS             complement(81..452)
                     /locus_tag="ON345_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            800..1672
                     /gene="rluF"
                     /locus_tag="ON345_003472"
     CDS             800..1672
                     /gene="rluF"
                     /locus_tag="ON345_003472"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR3073476.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGKRDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRYTSPGRKKKGR"
     gene            complement(1805..2077)
                     /locus_tag="ON345_003473"
     CDS             complement(1805..2077)
                     /locus_tag="ON345_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR3073477.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(2330..3679)
                     /gene="lysC"
                     /locus_tag="ON345_003474"
     CDS             complement(2330..3679)
                     /gene="lysC"
                     /locus_tag="ON345_003474"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR3073478.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4204..5853
                     /gene="pgi"
                     /locus_tag="ON345_003475"
     CDS             4204..5853
                     /gene="pgi"
                     /locus_tag="ON345_003475"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR3073479.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1520 a         1545 c         1511 g         1433 t
ORIGIN      
        1 ccagaaagcg tgcgtattgc ctgaaaatcc agccagctac agggtcgttc gcacgaaatc
       61 ttatttattc aacaaagccc cagtgcgctc accgcgcgac cccaggaaaa accgacaata
      121 tcgccaggct ccagcaagcg atccagcagt tgcgcgccgt gtaaccccat catcgccagt
      181 tgagtctctt catcctcatc atttcccgac accaccacaa cgtctttcag gccaaacttt
      241 tgcttcagtt gctgctccag ccagagattt tcgttgtagt catagttgat ggcgatggtg
      301 acaattccct gatcgcggcc tcgtttaagc aagcggctga tggtggtgcg gtaaataccc
      361 agttcgcgcg cgatttgcgc ctgcgtcata tcctgttcgt aatagagttg ggcaatcttc
      421 acaatcaaac ggatatcgtc actgttttcc attgccgcct cctggttctg cacatttgtg
      481 caaatacgct ttctcaccgc ttgatctgat ataaccccat catgagggcc tttcaaagcg
      541 atttttcact ctttgcatgg ggagaaatag cgctttctct tgcacatttg tttctctgcg
      601 tagtagtcat cagcacaggt gtgcagcgat tcgtaatatg ttgattatcc ttattttgcc
      661 aggcaaattt gcatcgtact tccggagaca cttttgtgat aaaaggcgtg gcagatcaca
      721 tttaatttcg acgttgccgc ataaccctgt ttttccccgc taagaggtac ggtatattcc
      781 gcgttttaca ggagaattta tgctgcccga ctcatcagtc cgtttaaata aatacatcag
      841 cgaaagcgga atttgctcac gccgcgaagc ggatcgctat atcgagcaag gcaatgtgtt
      901 ccttaatggc aagcgagcca ccattggcga tcaggtgaaa cccggcgacg ttgtgaaagt
      961 aaacggtcag ttgattgagc ctcgggaagc cgaagatttg gtacttatcg ccctgaacaa
     1021 gcccgtcggt attgtcagca ccaccgaaga tggcaagcgc gataacattg tcgatttcgt
     1081 taaccacagc aaacgcgtgt tcccgattgg ccgcctggat aaagactccc aaggactgat
     1141 tttcctcacc aatcacggcg atctggtgaa taagatcctc cgtgcgggta acgatcatga
     1201 gaaagagtat ctggtgacgg tcgataaacc gattaccgat gaatttattc gtggtatggg
     1261 ggcgggggtg ccgatcctcg ggacggtgac gaaaaagtgc aaagtcaaaa aagaagcgcc
     1321 gtttgtcttc cgcattaccc tggtgcaggg gctgaaccgt cagatccggc gcatgtgcga
     1381 gcatttcggc tatgaagtga aaaagctgga acgtacgcgc attatgaacg tcagcttaag
     1441 cggcattccg ctgggggagt ggcgcgattt aaccgacgat gagttaatcg acctctttaa
     1501 gctcatcgaa aattcctctt ccgaggcaaa acctaaagcg aaggccaaac cgaaaacagc
     1561 gggcatcaaa cgtccagtcg ttaagatgga aaaaacggcg gaaaaaggcg gtcgcccggc
     1621 gtccaacggt aagcgttata cctcgccggg gcgtaaaaag aagggacgct gattaacgcc
     1681 tcagactgat gaaaacgtcc aaaaagagct cggacagtcc cctcgcccct tcggggagag
     1741 ggttagggtg aggggaagat tgtgtccaca cagacgagtg aaaatatcag gcgttgattg
     1801 ttcattagcg cctgccgcgc gtcgacgtat ttgctgacca gctaaatgac gcttcaggat
     1861 ccggtttata agcctgcttt ttcttcaatt ggcgggcttt tttcgcttca gcctcacgct
     1921 cgaccattaa tttatcgatg tactctttct taatgctgtt ggtttcggag ttggtcagta
     1981 cgcgaccatg cgcaatccgc gcccgatcca gcagtgtttt aagttcacgc tgctcgcgtt
     2041 cagtcatctc tttttgggta atacggggga gtgccatgat ggtgccctca gtgagccaga
     2101 gagttcagtg tacgggaata cgtttgcgag cagaactgat aatattcatt ttgccttctc
     2161 ttaactattt atctctttcg ataattaatt agagatgcag ataaaaaaat taaaggcaat
     2221 tattctccga taaagacgtt ggttaacaga gtacaggctc gtttattgat gagcatagtg
     2281 acaagaaaat caatacggcc cgaaatatag cttccaggcc atacagtatt tactcaaaca
     2341 aattactatg cagtttttgc accacctgct cggcatcttc gccgggcacc aggaagcaca
     2401 ggttatggct ggatgcgccg taacaaatca tgcgaatgtt gaacggttcc agtacgccga
     2461 atacctcttt gccaacgccg caggcttttg acaggtcatt gccaatcaac gcgaccagcg
     2521 ccagaccttc ttccacctcc acccggcaca gtgcggaaag ctccatcagc agagattgcg
     2581 tcagcaacgt atcgccagtg gaggttgaac cggtggtatc aagggttaat gccacgctca
     2641 cttctgacgt ggtgattaag tctaccgaaa tattatgccg cgcgaggatg ccgaaaactt
     2701 ccgcgaggaa gccgcgagaa tgcagcatat tcaggctgtg caaagtgagc agagtctgat
     2761 tgcgacgaag cgccagcgcg cggaacagcg gcggattttc agttttattg cacaccagcg
     2821 taccacctgc gcgtgggtct ttgctggagc cgacaaagac cgggatatcg ctgcgtactg
     2881 cgggtagcaa cgttgccgga tgcagtactt ttgcaccaaa agttgccatc tctgccgctt
     2941 cggcaaacgc gatttcatca atgcgttttg ctgcggaaac tacgcgtgga tcggtggtgt
     3001 agatgcccgg gacgtcggtc cagatatcaa cacgagatgc gtgtaaagcc tccgccagca
     3061 aggctgccgt ataatcgctg cctccacggc caagcgtcgt tgtacgacct ttattttcgc
     3121 taccgataaa tccctgggtg atcactaagc cgtcattgag gcgtgggagc agctgcagcg
     3181 cggccagttc cgccagcgcg gctacatctg gctctgcacg accaaatcgg tcgttggtac
     3241 gcatcacttt acgtacatca aaccactgtg cctgaacatc gcgttcgcgc aggatttcga
     3301 caaacagcag ggtcgacatc agctcgccgt ggctgaccag ctcatctgtc agcgccggag
     3361 acgttgccag cgccgccgct tctgccagaa cagtaatgtt ctccagcaga cgttcaatct
     3421 cttcacggat aacgttcggg taacgcagac gttccagaat ggcaaactgg atgttgcgaa
     3481 tagcgtcgag tttttcgaat cgctcgccag gttccagccc ttccgctaaa gcgaccagca
     3541 gattagtgat accagcagaa gccgagagga ctactaaacg cacgttggca tcagaaagca
     3601 caatatcagc gctgcggttc atggcgtcaa aatcagctac gctggtaccg ccaaatttgg
     3661 agacaacaat ttcagacata actacctcgt gtcaggggat ccattttcag ccttggcaca
     3721 agggaagagc ggaagacggg tgggcgcaga gcgatacttc gctactattt tcacccagaa
     3781 gtgctccacc acttgcgaaa cgcccgactg cgaacgcttc tggtgacaac ccaggggatt
     3841 cagcccctgt agccgatgat gaacgtggcc agccgttcaa tcacctcggc gatgcacccc
     3901 ctcaggtgtt atcacaggac tggctcctcc aacaccgtta cttgggcaac gcgcctcttc
     3961 tggcctgcgc tagcgcaggt agtacattta taaataaagg gtgagcgggg cggttgtcaa
     4021 cgacggggtc atgcggattt ttcatccact catggcggtc agtagttcag ctaataaatg
     4081 cttcactgcg ctaagggttt acactcaaca ttacgctaac ggcactaaaa ccatcacatt
     4141 tttctgtgac tggcgctaca atcttccaaa gtcacaattc tcaaaatcag aagagtattg
     4201 ctaatgaaaa acatcaatcc aacgcagacc gctgcctggc aggcactaca gaaacacttc
     4261 gatgaaatga aagacgttac gatcgccgat ctttttgcta aagacggcga tcgtttttct
     4321 aagttctccg caacattcga cgatcagatg ctggtggatt actccaaaaa ccgcatcact
     4381 gaagagacgc tggcgaaatt acaggatctg gcgaaagagt gcgatctggc gggcgcgatt
     4441 aagtcgatgt tctctggcga gaagatcaac cgcactgaaa accgcgccgt gctgcacgta
     4501 gcgctgcgta accgtagcaa taccccgatt ctggttgatg gcaaagacgt aatgccggaa
     4561 gtcaacgcgg tgctggagaa gatgaaaacc ttctcggaag cgattatctc cggtgagtgg
     4621 aaggggtata ccggcaaagc aatcactgac gtagtgaaca tcgggatcgg cggttctgac
     4681 ctcggcccgt acatggtgac cgaagcactg cgtccgtaca aaaaccacct gaacatgcac
     4741 tttgtttcta acgtcgatgg gactcacatc gcggaagtgc tgaaaaaagt aaacccggaa
     4801 accacgctgt tcctggtagc atctaaaacc ttcaccactc aggaaaccat gaccaacgcc
     4861 catagcgcgc gtgactggtt cctgaaagcg gcaggtgatg aaaaacacgt tgcgaaacac
     4921 tttgcggcgc tctcaaccaa tgccaaagcc gttggcgagt ttggtattga tactgccaac
     4981 atgttcgagt tctgggactg ggtcggcggc cgttactctt tgtggtcagc gattggcctg
     5041 tcgattgttc tctccatcgg ctttgataac ttcgttgaac tgctttctgg cgcacacgcg
     5101 atggacaagc atttctccac cacgcctgcc gagaaaaacc tgcctgtact gctggcgctg
     5161 attggcatct ggtacaacaa tttctttggt gcggaaactg aggcgattct gccgtatgac
     5221 cagtatatgc accgtttcgc ggcgtacttc cagcagggca atatggagtc caacggtaag
     5281 tatgttgacc gtaacggtaa cgttgtggat taccagactg gcccgattat ctggggtgaa
     5341 ccaggcacta acggtcagca cgcgttctac cagctgatcc accagggaac caaaatggta
     5401 ccgtgcgatt tcatcgctcc ggctatcacc cataacccgc tctctgatca tcaccagaaa
     5461 ctgctgtcta acttcttcgc ccagaccgaa gcgctggcgt ttggtaaatc ccgcgaagtg
     5521 gttgagcagg aatatcgcga tcagggtaaa gatccggcaa cgcttgacta cgtggtgccg
     5581 ttcaaagtgt tcgaaggtaa ccgcccgacc aactccatcc tgctgcgcga aatcactccg
     5641 ttcagcctgg gggcgttgat tgcgctgtat gagcacaaaa tctttactca gggcgtgatc
     5701 ctgaacatct tcaccttcga ccagtggggc gtggagctgg gtaaacaact ggcaaaccgt
     5761 attctgccag agctgaaaga tgataaagaa atcagcagcc acgatagctc gaccaatggt
     5821 ctgattaacc gctataaagc gtggcgcggt taatttaaaa accttaataa caatgccgac
     5881 gttatgtcgg cattgtttta tcagataaat ccccttgtct gtgatttaac ggaaatcata
     5941 ccgtgaggtt aatcctaaaa tagattttta atcgtgattg atttcggaaa atactcagat
     6001 taattactt
//
LOCUS       DAJSKJ010000199         5989 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000199 DAJSKJ010000000
VERSION     DAJSKJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.203"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            135..1241
                     /gene="ompC"
                     /locus_tag="ON345_003476"
     CDS             135..1241
                     /gene="ompC"
                     /locus_tag="ON345_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR3073480.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVRGEGMTNNGRSALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1353..2408
                     /gene="apbE"
                     /locus_tag="ON345_003477"
     CDS             1353..2408
                     /gene="apbE"
                     /locus_tag="ON345_003477"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR3073481.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2482..3546
                     /gene="ada"
                     /locus_tag="ON345_003478"
     CDS             2482..3546
                     /gene="ada"
                     /locus_tag="ON345_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR3073482.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEELAAGFHPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLLESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGND
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3546..4196
                     /gene="alkB"
                     /locus_tag="ON345_003479"
     CDS             3546..4196
                     /gene="alkB"
                     /locus_tag="ON345_003479"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR3073483.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4272..5915
                     /gene="yojI"
                     /locus_tag="ON345_003480"
     CDS             4272..5915
                     /gene="yojI"
                     /locus_tag="ON345_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR3073484.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKYALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1417 a         1516 c         1628 g         1428 t
ORIGIN      
        1 gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga
       61 ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac
      121 agagggttaa tgacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag
      181 caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg
      241 gtaaagtaga cggcctgcac tatttctctg acaacaagtc agaagacggc gaccagacct
      301 atgtacgtct tggtttcaaa ggcgaaactc aggttactga ccagctgacc ggttacggcc
      361 agtgggaata tcagatccag ggcaatacct ctgaagacaa caaagaaaac tcctggaccc
      421 gtgtggcatt cgcaggtctg aaattccagg atgtgggttc tttcgactac ggtcgtaact
      481 acggcgttgt ttacgacgta acttcctgga ccgacgtact gccagaattc ggtggcgaca
      541 cctacggttc tgacaacttc atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca
      601 ctgacttctt cggtctggtt gacggcctga actttgctgt tcagtaccag ggtaaaaacg
      661 gtagcgtaag aggcgaaggc atgaccaaca acggtcgtag tgctctgcgt cagaacggcg
      721 acggcgttgg cggatctatc acttatgatt acgaaggctt cggtatcggt ggtgcgatct
      781 ccagctccaa acgtactgat gatcagaaca gcccgctgta catcggtaac ggcgaccgtg
      841 ctgaaaccta caccggtggt ctgaaatacg acgctaacaa catctacctg gctgctcagt
      901 acacccagac ctataacgca actcgcgtag gttccctggg ttgggcgaac aaagcacaga
      961 actttgaagc tgttgctcag taccagttcg acttcggtct gcgtccgtct gtagcatacc
     1021 tgcagtctaa aggtaaaaac ctgggtacca tcgctggtcg taactacgac gacgaagata
     1081 tcctgaaata tgttgatgtt ggtgctacct actacttcaa caaaaacatg tccacctacg
     1141 ttgactacaa aatcaacctg ctggacgaca accagttcac tcgtgacgct ggcatcaaca
     1201 ctgataacat cgtagctctg ggtctggttt accagttcta atctcgattg atatcgaaca
     1261 aagggcctgc gggccctttt ttcattgttt tcagcgtaca aactcatttt tttggtgtac
     1321 tcttgcggcc gttcgcatga ggataatcac gtatggaaat aagctttacc cgcgtggcac
     1381 tgctggctgc cgcgctcttc tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc
     1441 acgctactga agttaccgtt cttgaaggca aaaccatggg taccttctgg cgtgccagca
     1501 tcccgggcat tgatgccaaa cgcagtgccg aacttaaaga aaagattcag acccagctgg
     1561 acgccgacga tcagctgctt tcgacctata aaaaagattc cgcgctgatg cgctttaacg
     1621 actcgcaaag tttgtcgccg tggccggtaa gtgaagcgat ggccgaaatc gtcactacct
     1681 cgctgcgcat tggcgcgaag accgatggtg cgatggatat taccgtcggg ccgctggtaa
     1741 atctgtgggg ctttggcccg gaacaacagc cggttcaaat tccgagccag gaacagatcg
     1801 acgcgatgaa agccaaaact ggtttacagc accttacggt cattaatcag tcgcatcagc
     1861 aatatctgca aaaagacctg ccggatttat atgtcgatct ctctaccgtc ggcgaaggtt
     1921 atgcggcgga tcacttggca cgcttgatgg agcaggaagg gatttcccgc tatctggtgt
     1981 cggtgggcgg cgcgctgaac agccgtggta tgaacggtga aggcctgccg tggcgggtag
     2041 cgattcaaaa accaaccgat aaagaaaacg cagttcaggc tgtggtggat atcaacggtc
     2101 atgggattag cacctctggc agctaccgta actattacga actggacggc aaacgtcttt
     2161 cccatgttat cgatccgcaa accgggcgtc ccatcgaaca caatctggta tctgtgacgg
     2221 tgattgctcc gacggcgctg gaagccgatg cctgggatac tggcttgatg gtactcgggc
     2281 cggagaaagc caaagaggtt gttcgccggg aagggctggc ggtttatatg atcaccaaag
     2341 aaggcgatag ctttaaaacc tggatgtcac cacagtttaa aagcttcctt atcagcgaaa
     2401 aaaattaaag cgcaagattg ttggtttttg cgtgatggtg accgggcaga ctaaaggcta
     2461 tccttaacca gggagctgat tatgaaaaac gccacatgct taactgacga tcaacgctgg
     2521 caatctgtct tagcccgcga cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc
     2581 acgggtatct tttgccgtcc gtcttgccgc gccagacatg ccttgcggga aaatgtctcc
     2641 ttctacgcaa atgccagcga ggaactcgcc gccggatttc acccctgcaa acgttgtcag
     2701 ccagacaaag ccaatccccg gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg
     2761 ctggaacagg aaacgcctgt aacgctggaa gccttagccg accaggtggc gatgagtcca
     2821 tttcatctac atcggttgtt taaagcgact accggaatga cgcctaaagc ctggcaacag
     2881 gcctggcgcg ctcgccgttt gctcgaatcg ctggcgaaag gggagagcgt gacgacgtct
     2941 attcttaacg ccggattccc cgacagcagc agttactacc gcaaagccga tgaaacgctg
     3001 ggcatgacgg ctaaacaatt ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg
     3061 gctgattgtg agctgggtcg ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata
     3121 ttgctgggca atgatgacgc gacattaatc agcgagttgc agcagatgtt tcccgctgcc
     3181 gacaacgcgc ctgccgatct gacgtttcag caacatgtgc gtgaagtgat cgccagcctc
     3241 aatcaacgcg atacgccgct gacgttaccg ctggacattc gcggcactgc ttttcagcaa
     3301 caagtctggc aggcactgcg cacgatacct tgcggtgaaa ccgtcagtta tcagcaactg
     3361 gctaacgcca tcggcaaacc gaaagcggta cgggccgttg ccagtgcctg tgccgccaac
     3421 aagctggcta tcgtaatacc ctgtcatcga gtggtccgtg gtgatggcac actttccggt
     3481 taccgctggg gcgtgtcgcg taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag
     3541 aggtaatgtt ggatctgttt gccgatgctg aaccgtggca agagccactg gcggctggtg
     3601 cggtaatttt acggcgtttt gcttttaacg ctgcggagca actgatccgc gatattaatg
     3661 acgttgccag ccagtcgccg tttcgccaga tggtcacccc cgggggatat accatgtcgg
     3721 tggcgatgac caactgtggg catctgggct ggacgaccca tcggcaaggt tatctctatt
     3781 cgcccattga tccgcaaaca aataaaccgt ggcccgccat gccacaaagc tttcatgatt
     3841 tatgtcaacg tgcggctacg gcggcgggct atccagattt ccagccagat gcttgtctta
     3901 tcaaccgcta cgctcctggc gcgaaactgt cgctgcatca ggataaagac gaaccggatc
     3961 tgcgcgcgcc aattgtttct gtttctctgg gcttacccgc gatttttcaa tttggtggcc
     4021 tgaaacgtaa tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg
     4081 gcggtgaatc gcggctgttt tatcacggta ttcaaccgtt gaaagcgggg tttcatccac
     4141 tcaccaccga ctgccgctac aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat
     4201 aagaattatt attgctgtgc gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc
     4261 gggttttctg catggaactt cttgtacttg tctggcggca gtatcgctgg ccatttatca
     4321 gtgtgatggc gctaagcctc gccagtgcgg cattaggcat tggcttaatt gcttttatca
     4381 atcagcgcct tatcgaaacg gcggatacca gtctgctggt gttgccggag tttctgggat
     4441 tattgctgct gttgatggca gtcactctcg gatcgcaact ggcgctcacc actttggggc
     4501 atcactttgt ttaccgactg cgtagtgaat ttatcaagcg gattctggat acccacgtcg
     4561 agcgcattga acaactcggt agcgcctcgt tgctagcggg attaaccagc gatgtgcgca
     4621 atatcaccat tgcttttgtg cgtctgccgg aactggtgca ggggatcatt ctcactatcg
     4681 gttccgcggc gtatctgtgg atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga
     4741 tggcgatcac catctggggc ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga
     4801 ccctgcgtga aaccgaagac aagctgtaca cggattttca aaccgtactc gaagggcgca
     4861 aagagctgac tctgaaccgg gaacgcgccg agtatgtgtt taacaacctc tacatccccg
     4921 atgcgcaaga gtatcgccat catattattc gcgcagacac cttccatctt agcgccgtga
     4981 actggtcaaa catcatgatg ctgggcgcta tcggcctggt gttctggatg gcgaacagtc
     5041 tcggttgggc tgataccaac gttgctgcga cctattcgtt gacgctgtta ttcctgcgta
     5101 cgccgctgct ttcggcggtt ggcgcattgc cgacgctgct gacggcgcag gtggcgttta
     5161 acaagctgaa caaatacgcg ctcgcgcctt tcaaagctga gtttccgcgc ccgcaggctt
     5221 ttcccaactg gcaaacgctg gagctgcgta acgtgacgtt tagctatcag gataacgcgt
     5281 tttccgttgg gccgattaac ctcaccatta aacgtggcga gctgctgttt ctgattggtg
     5341 gcaacggtag tggaaaatcg acgctggcga tgttgctgac gggcttgtac cagccacaaa
     5401 gcggcgaaat cttgctggat ggcaaacctg tcagtggcga acaaccggaa gattatcgca
     5461 aactgttttc ggcagtgttt accgatgtct ggctgtttga tcaactgctg gggccggagg
     5521 gtaaacccgc taacccgcaa ctggttgaga agtggctggc gcagctgaaa atggctcata
     5581 agcttgagtt aagcaacggg cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac
     5641 gcgtggcgct gttgctggcg ctggcagaag aacgcgatat tatcctgctg gatgaatggg
     5701 cggcggatca ggatccacac ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc
     5761 aggagatggg taaaactatt ttcgctatca gtcatgatga tcattacttt atccacgccg
     5821 accgcctgct ggaaatgcgc aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg
     5881 ccgcttcgcg tgatgccgtt gcccggacgg cataatatta cgacgctgac ctggcggact
     5941 gctgccgtca ggtcaatatg ctaaaaatcc cttcatttct aataaccct
//
LOCUS       DAJSKJ010000200         5914 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000200 DAJSKJ010000000
VERSION     DAJSKJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.204"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..758)
                     /gene="melB"
                     /locus_tag="ON345_003481"
     CDS             complement(<1..758)
                     /gene="melB"
                     /locus_tag="ON345_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR3073485.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITG"
     gene            complement(861..2216)
                     /gene="melA"
                     /locus_tag="ON345_003482"
     CDS             complement(861..2216)
                     /gene="melA"
                     /locus_tag="ON345_003482"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3073486.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAKELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLATYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2499..3407
                     /gene="melR"
                     /locus_tag="ON345_003483"
     CDS             2499..3407
                     /gene="melR"
                     /locus_tag="ON345_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR3073487.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3606..5873
                     /gene="adiA"
                     /locus_tag="ON345_003484"
     CDS             3606..5873
                     /gene="adiA"
                     /locus_tag="ON345_003484"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR3073488.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1552 a         1569 c         1421 g         1372 t
ORIGIN      
        1 aagccggtaa taatgttgct ggctacatta taagcaagag ccatacccaa gaggcatgaa
       61 agctgatcgt ttttataaat tagcgcaacg atggctttaa gtgtcagatg gcttccttca
      121 gcagacggtt gattgtctga cgaaaagact tcatgcacat tgcgcagagt gatgatggtt
      181 gaaacaataa aaaaggcgat cagtaccaga gtgaacatct gaaagccaaa tccccgatcg
      241 ccaccgccga cataattaac aaatggtagc gtcacacctg ccgtaacaaa gcctgccaga
      301 ctggcaaaaa aacgcggata aggaaccagt tgttcgcgct cacgtttatc gagcgtgatg
      361 gttggaacca gcgaccagaa gggaatatcc ataatggtgt aagtcatgcc ccagaggatg
      421 taggtcacgc aaacaaagac aatctgagta gtaccttcaa acagatgcgc actaaagagg
      481 agaaataaga ttacagagtt tgccaacgta ccgatcagga tccagggttt gaacttaccc
      541 catcgcgatc gcgtagcatt tacaatccat cccataatcg gatcgttaat agcatcccag
      601 atcctcgcca ccagaaacaa agtaccgacc aaacccacag acagcccgac gacatcggtg
      661 tagtaataca tgaggtacat atacacaatg ccgatcgcaa aatccttccc gaacgctcca
      721 aatccataac tgagttttgt agtcattgaa atgctcatag ggtatcgggt cgctgttacc
      781 aacgcccgcc ttctgttatt ggtatcgtca gaggtgtgcg ccggaataaa tcccccggcg
      841 cagtagcgtt tagtcgcgtt ttaacggtgc aaccagcctg gcagccagtc gccgtgggcg
      901 gcaatcaggt cgtcaacaag agcatatatt tcgtcaatgc ccagcacggc ggcagtatgc
      961 gggtccatca tcgcggcgtg gtaaacacgg tcgcgatttt ccgtaagaat agcttcggtc
     1021 agcagcgtct gtacgttgat gttggtttgc atcagggcgg ccagatgcga aggtagcgta
     1081 ccgactttgg ttggctgaat gccattagca tcaaccagac aggctacttc cacgcaacat
     1141 ccttgtggca ggttatcaat caaaccatcg ttacggacgt tgccgtaaat cacactcggc
     1201 tcgccagtcc agatagcgtt catgattgtg ctggcatatt cccgtgacgg tttaatatca
     1261 atccgggagg cgtttttata ctcctccagc tctttatgcc agttcgccag ctgctcgacg
     1321 cagcgtttcg ggtactcatc cagcggtact ttataacgct caatcaaatc ctcacgacct
     1381 ggcttaataa accacggcgt atattcggca aaatgttctg acgattccgt gacgaagtag
     1441 cccagctttt tgaacatttc gtagcgcaca atattctggc agcgagtatt gccgtgaata
     1501 ttcggcttcg gtgcctgccc tgcttcataa gtcgccagca gttccgggta gagattcacg
     1561 taactgccgt cggcggtttt gcgctccagc tccaggtaaa acgccatatg gttgatacct
     1621 gcgcaacggt aacgcagcgt agctgggtcg atattgagat cacgcgccag ctctttcgcc
     1681 gttccctgca ccgaatggca cagcccgacc tgtttgatat gcggatagcg ggcatacatc
     1741 gcccaggtat tcatcgccat tgggttaacg tagttgagca atgtggcatc ggggcagact
     1801 tccgtcatgt cctcgcaaat ttgccacaga tgcggaatgg tacgtagcgc gcgcataata
     1861 ccgcccggcc ccaacgtatc ggcaatggtt tgttccagac catgccgctt acagacctcg
     1921 aaatcagtca ccgtgcaagg ttcataaccg ccaatctgaa atgccaccac gacaaaatcg
     1981 gcatcctcta aggcttcttt ctgttgggtg tggcaggtga ttttgccgct ggcccctgct
     2041 gaatccatca gcttacgcac cacaatatgc gactcttcca ggcgggtggg atcaatgtcc
     2101 atcagggcaa tatgcgccgt tttcagcgcc tcgcgatgga acacatcacc aagaatattt
     2161 ttaacgaaaa tcgtcgaacc agcgccgata aatgtaattt tgggtgcagt catcatgcag
     2221 atctcctggc ttgcttgaat aacttcatca tggcaggcga atctgcagaa aacctccggc
     2281 ttccaggttg agcattccca taaactcaga tcctgcgtga agcagcagta aatctgagtt
     2341 tatgggaata agttacctga aaagcagagt tttcggcctc atgatgctcg gacgctcccc
     2401 tcgcctctct gggaagaggg tgaaaaccgt gctcccactc gcagtcatcc tccctcactc
     2461 ctgccataat tctgatattc caggaaagag agccatccat gaatacagat acgtttatgt
     2521 gcagcagcga cgaaaaacag acccgcagcc cgctgtcgct gtattcggaa taccagcgga
     2581 tggagattga gtttcgcgca ccgcatatca tgcccaccag ccactggcat ggtcaggtcg
     2641 aagtgaatgt gcctttcgat ggcgatgtgg aatacctgat caacaatgaa aaagtgaata
     2701 tcaatcaggg tcatatcacg ctgttctggg cctgtacacc gcaccaacta acagataccg
     2761 gaacctgtca gagcatggcg atttttaatc tgccgatgca tctgtttctc tcctggccgc
     2821 tggataaaga cctgattaac cacgtcactc acggcatggt gatcaaatca ctggccacac
     2881 agcaacttag cccgtttgaa gtgcgccgct ggcagcagga attaaacagt ccgaacgagc
     2941 aaattcgcca gctcgccatt gatgaaattg gcctgatgct caagcgattt agcctctctg
     3001 gctgggaacc gattctggtc aataaaacct cgcgcacaca caaaaacagc gtctcgcgcc
     3061 atgcgcaatt ttatgttagc cagatgctgg gctttattgc cgaaaactat gatcaggcgc
     3121 tgaccatcaa cgatgtggct gagcacgtca aacttaacgc caactatgca atggggatat
     3181 tccagcgggt catgcaattg acgatgaaac agtacattac cgcgatgcgc atcaaccacg
     3241 ttcgcgcgtt actgagcgat accgataaaa gtattctcga tattgccctg acggcaggct
     3301 ttcgttcaag tagtcgtttt tacagcacgt tcggcaaata tgtcggcatg tcgccgcaac
     3361 aataccgcaa acttagccaa cagcgccgcc agacgtttcc cggctaaaga cgatatcagt
     3421 atcagccaaa aaaatagttt agccgtcgat aataagcagt gaaaataatt taaaataatc
     3481 acataacgta ttgtttataa aactaaatat ttcaaaaatg tttgtttttc acgcgcttta
     3541 cagcccgaaa aggccggaag atacttgccc gcaacgaaga ttccttcata accgggtaag
     3601 caatgatgaa agtattaatt gttgaaagcg agtttctcca tcaagacacc tgggtcggta
     3661 acgccgttga gcgtctggca gatgctttaa gccagcaaaa tgttaccgtg attaaatcca
     3721 cctcctttga tgatggtttt gccattctct cttcaaacga agccattgac tgcctgatgt
     3781 tcagctatca aatggaacat ccggacgaac atcaaaacgt cagacaattg atcggtaagc
     3841 ttcatgagcg ccaacaaaac gtgccggtct tcctgttggg cgatcgggaa aaagccctcg
     3901 ccgcaatgga tcgcgacctg ctggagcttg tcgatgaatt cgcctggatt ctggaagata
     3961 ccgccgactt tatcgccgga cgcgccgttg ccgcgatgac ccgctaccgc cagcagctgt
     4021 tgccgccact gttcagcgcg ctgatgaaat atagtgacat ccatgaatat tcctgggcag
     4081 cgccaggcca ccagggcggc gttggtttta ccaaaacacc cgccggacgt ttctaccatg
     4141 actactatgg tgaaaatctg ttccgcaccg acatgggcat cgaacgaact tccctcggtt
     4201 ctttgcttga ccatactggc gcatttggcg aaagcgaaaa atatgccgca cgcgtatttg
     4261 gtgccgatcg ctcctggtcg gtagtcgtcg gtacttccgg ctctaaccgc accatcatgc
     4321 aggcttgcat gaccgataac gatgtcgtgg tcgttgaccg taattgccat aaatccatcg
     4381 aacaaggctt gatgctgaca ggcgcgaaac cagtctacat ggtgccaagc cgcaaccgct
     4441 acggcattat cgggccaatc tatccgcagg aaatgcaacc tgaaaccttg cagaagaaaa
     4501 tcagtgaaag cccgctgact aaagacaaag ctgggcaaaa accgtcttac tgcgtggtga
     4561 ccaactgcac ctatgacggc gtgtgttata acgctaaaga agcgcaggat ctgctggaaa
     4621 aaacctccga tcgtctgcac tttgacgaag cctggtacgg ctatgcacgt ttcaacccga
     4681 tctatgccga tcactatgcc atgcgcggcg aacctggcga tcacaacggt cctaccgttt
     4741 tcgccaccca ctccacccac aaactgctga atgcgctgtc acaggcttct tatattcatg
     4801 tacgtgaagg tcgtggggcg attaacttct cccgcttcaa ccaggcctac atgatgcatg
     4861 ccaccacctc cccgctgtat gccatctgcg catccaacga cgtggcggtg tcgatgatgg
     4921 acggcaacag cggcctgtca ctgacacaag aagtgattga cgaagcggtt gattttcgtc
     4981 aggcgatggc gcggctgtat aaagagttca ccgctgacgg tagctggttc ttcaaaccgt
     5041 ggaacaaaga agtcgtcacc gacccacaaa ccggcaaaac ctatgacttt gctgacgcac
     5101 caaccaaact gctgaccacc gttcaggact gctgggtaat gcatccgggc gaaagctggc
     5161 acggcttcaa agatattccg gataactgga gtatgctcga cccgattaaa gtcagcatcc
     5221 ttgctccggg aatgggtgaa gatggtgaac tggaagaaac cggtgttccg gcggcgctgg
     5281 tcactgcctg gcttggtcgc cacggcattg tgcctacccg caccactgac ttccaaatta
     5341 tgttcctgtt ctctatgggc gtaacccgtg ggaaatgggg aactctggtt aacacccttt
     5401 gctccttcaa acgccactat gacgccaaca caccgctggc gcaggtgatg ccggaacttg
     5461 ttgaacaata tcctgacact tacgcgaaca tggggattca cgatctgggt gacaccatgt
     5521 ttgcctggct gaaagaaaac aaccctggcg cacggttgaa cgaagcctat tccggcctgc
     5581 cggtggcgga aatcaccccg cgtgaagcgt acaacgcgat tgtcgacaac aatgtcgaac
     5641 tggtatccat tgaaaatctg ccaggacgca tcgcggcaaa ctcagttatc ccatatccgc
     5701 caggaatccc gatgctgctg tctggtgaaa acttcggcga taaaaacagt ccgcaagtaa
     5761 gttatttacg ctcgctgcaa tcctgggacc accatttccc tggatttgaa cacgaaactg
     5821 aagggactga aattattgac ggtatttacc acgttatgtg tgtgaaagcg taaccactat
     5881 tccgctgaag gcgtaattgt ttaaataaca ttac
//
LOCUS       DAJSKJ010000201         5806 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000201 DAJSKJ010000000
VERSION     DAJSKJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.205"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            120..2795
                     /gene="adhE"
                     /locus_tag="ON345_003485"
                     /gene_synonym="adhC"
     CDS             120..2795
                     /gene="adhE"
                     /locus_tag="ON345_003485"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR3073489.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            2919..3815
                     /locus_tag="ON345_003486"
                     /pseudo
     CDS             2919..3815
                     /locus_tag="ON345_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(4097..4714)
                     /gene="tdk"
                     /locus_tag="ON345_003487"
     CDS             complement(4097..4714)
                     /gene="tdk"
                     /locus_tag="ON345_003487"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR3073490.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5319..5732
                     /gene="hns"
                     /locus_tag="ON345_003488"
     CDS             5319..5732
                     /gene="hns"
                     /locus_tag="ON345_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR3073491.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT         1553 a         1452 c         1357 g         1444 t
ORIGIN      
        1 tgtgagcgcg agtaagcttt tgattttcat aggttaagca aatcatcacc gcactgacta
       61 tactctcgta ttcgagcaga tgatttacta aaaaagttta acattatcag gagagcatta
      121 tggctgttac taatgtcgct gaacttaacg cactcgtaga gcgtgtaaaa aaagcccagc
      181 gtgaatatgc cagtttcact caagagcaag tagacaaaat cttccgcgcc gccgctctgg
      241 ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt tgccgaatcc ggcatgggta
      301 tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga atatatctac aacgcctata
      361 aagatgaaaa aacctgtggt gttctgtctg aagacgacac ttttggtacc atcactatcg
      421 ctgaaccaat cggtattatt tgcggtatcg ttccgaccac taacccgact tcaactgcta
      481 tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat catcttctcc ccgcacccgc
      541 gtgcaaaaga tgccaccaac aaagcggctg atatcgttct gcaggctgct atcgctgccg
      601 gtgctccgaa agatctgatc ggctggatcg atcaaccttc tgttgaactg tctaacgcac
      661 tgatgcacca cccagacatc aacctgatcc tcgcgactgg tggtccgggc atggttaaag
      721 ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc gggcaacact ccagttgtta
      781 tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt actgatgtcc aaaaccttcg
      841 acaacggcgt aatctgtgct tctgaacagt ctgttgttgt tgttgactct gtttatgacg
      901 ctgtacgtga acgttttgca acccacggcg gctatctgtt gcagggtaaa gagctgaaag
      961 ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc ggctatcgtt ggtcagccag
     1021 cctataaaat tgctgaactg gcaggcttct ctgtaccaga aaacaccaag attctgatcg
     1081 gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca tgaaaaactg tccccgactc
     1141 tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga aaaagcagag aaactggttg
     1201 ctatgggcgg tatcggtcat acttcttgcc tgtacactga ccaggataac caaccggctc
     1261 gcgtttctta cttcggtcag aaaatgaaaa cggctcgtat cctgattaac accccagcgt
     1321 ctcagggtgg tatcggtgac ctgtataact tcaaactcgc accttccctg actctgggtt
     1381 gtggttcctg gggtggtaac tccatctctg aaaacgttgg tccgaaacac ctgatcaaca
     1441 agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca caaacttccg aaatctatct
     1501 acttccgccg tggctccctg ccaatcgcgc tggatgaagt gattactgat ggccacaaac
     1561 gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg ttatgctgat cagatcactt
     1621 ccgtactgaa agcagcaggc gttgaaactg aagtcttctt cgaagtagaa gcggacccga
     1681 ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc cttcaaacca gacgtgatta
     1741 tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat catgtgggtt atgtacgaac
     1801 atccggaaac tcacttcgaa gagctggcgc tgcgctttat ggatatccgt aaacgtatct
     1861 acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc tgtcaccacc acttctggta
     1921 caggttctga agtcactccg tttgcggttg taactgacga cgctactggt cagaaatatc
     1981 cgctggcaga ctatgcgctg actccggata tggcgattgt cgacgccaac ctggttatgg
     2041 acatgccgaa gtccctgtgt gctttcggtg gtctggacgc agtaactcac gccatggaag
     2101 cttatgtttc tgtactggca tctgagttct ctgatggtca ggctctgcag gcactgaaac
     2161 tgctgaaaga atatctgcca gcgtcctacc acgaagggtc taaaaatccg gtagcgcgtg
     2221 aacgtgttca cagtgcagcg actatcgcgg gtatcgcgtt tgcgaacgcc ttcctgggtg
     2281 tatgtcactc aatggcgcac aaactgggtt cccagttcca tattccgcac ggtctggcaa
     2341 acgccctgct gatttgtaac gttattcgct acaacgcgaa cgacaacccg accaagcaga
     2401 ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg ttatgctgaa attgctgacc
     2461 acctgggtct gagcgcaccg ggcgaccgta ctgctgctaa gatcgagaaa ctgctggcat
     2521 ggctggaaac gctgaaagct gaactgggta ttccgaaatc tatccgtgaa gctggcgttc
     2581 aggaagcaga cttcctagcg aacgtggata aactgtctga agatgcattc gatgaccagt
     2641 gcaccggcgc taacccgcgt tacccgctga tctccgagct gaaacagatt ctgctggata
     2701 cctactacgg tcgtgattat gtagaaggtg aaactgcagc gaagaaagaa gctgctccgg
     2761 ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt agcgctgtct ggcaacataa
     2821 acggcccctt ctgggcaatg ccgatcagtt aaggattagt tgaccgatcc ttaaactgag
     2881 gcactataac ggcttccaca acagggagcc gttttcttat gccacttctc aatgatctgc
     2941 tcgatttcag tgaccatccg cttatgcctc cgccctctgc acaactattt gcagaacacc
     3001 ttcccaccga gtggatacaa cactgcctga cgctttctgc tcatgcgacc gttcgccgcc
     3061 gtcgtttacc gggggacatg gttatctgga tggtggtgca atgagccaat taccgatgtt
     3121 gttcgccgtc tgaacctgag cgcggatggc gaagcgggga tgaacctgct ggcccgcagc
     3181 gctgtcaccc aggcgcgtca gcgcgtgggg gccgccccag tggaatggct cttccgccag
     3241 accgcacaga caggggcgcg gaacgttacc tgaaggatga ctggcacggc ctgcaacttt
     3301 ttgccattga tggcgcacag ttcaggacac ctgataaacc cgagctgcgt gaatattatg
     3361 ggtctgccaa cacatccact aagcggcaga acgcctaccc ggttatgcgt ctggtggcct
     3421 taatgaacct gggaagtcac attctgctga atgccgtgac cgcaccttac cggcagagcg
     3481 aaactgtgct ggcccactcc atgctcgcca ccatcccgga taactctatt acgttgtttg
     3541 ataaactttt ttacagcgaa gacctgctgc tgacgctgaa ccagaagggt tgtaaccgcc
     3601 actggctact gcctgcgtgg aagaatatcg catcagaaat gatagagctg ggaaacacgg
     3661 cgtcaccagg gaccatcccg aaacgactgg agcatcttcg gggtgctctg gaagtggtgt
     3721 tcataacaaa acgcccccga ccatcaaggc cgaggtcggt taagatatca aaaacccgtt
     3781 atcctgtgaa acatagtgcc gctcctctta agtgaacggc attgcccttc tggggccgta
     3841 ttttttgttt acccaaagca acttttccat aaaccgacag cattagcctt catcatattt
     3901 gcgacgatgt ataacgccta aacacaggga tattgtactt tacaggtcac aagtcaacgt
     3961 cggtgcttaa gagccctgtg aggcgtatag cggcgttaaa aaactgccga gaagggtata
     4021 tagcccggaa gaagtgcgta aaacgaactg acaggataaa agtgccctgc tcaccctgtc
     4081 agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg aatagccgtt aatgagccga
     4141 cttgtaacgc ctctttatag tgtttacggc atacagaaac gtatcgttca ttgccaccaa
     4201 ttaccacctg ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc agcaccatgc
     4261 ttgctttacg gccacaaaaa cagatggttt ttaattcaac cagtttgtcg gaccatgcca
     4321 gtaagtattg gctgccaata aataattcac ctcgaaaatc ggtacgtaaa ccataacaaa
     4381 gtacgggtat atcgagttga tcgacaacct ccgataattc atatacttgt tgtctggtta
     4441 aaaactggca ttcatcaacc agtacgcaat gaattgcctg ctgttcatgt tccgcacgaa
     4501 tctcatcaaa taatgatgaa ttttggttaa ataattttgc aggcgatgac aaacctatac
     4561 gcgaactgac tttcccggca ccaaagcgat catcaatttc tgccgtatat acgacagtgc
     4621 gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac ttacccgcat
     4681 tcattgcgga atagtagaaa tatagctgtg ccatcagcca caggccctca atgatatgaa
     4741 ataaagttgg ctggagttta tcataattcg tcagtttttc agctcaacag tatgcacaga
     4801 agtgggtata tgcgttctcc cttacgaagc cttgcataat ccttctgagc tatcattaca
     4861 actgcctcgc ttgttataag cgggtaaatg actgctggta actattcaca atctttaacc
     4921 tgttgcgcaa gtaatagccc tctgttgacc tccaggagat agtgcaatac taagtccatg
     4981 ctcttattgc gactgttcta cttttcatca ttcgcttaat agggaattct cgtaaacaca
     5041 actaatacag aagactgaaa ggtcgtcagc ctacgataat ctccccataa aatgtgacat
     5101 gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg gtgtaaatag ggctatatgc
     5161 cgcgtctttt ctggctaatt ttatgaaaag atatttattg gcggcacaaa ataaagaaca
     5221 attttgaatt ccttacattc ctggctattg cacaactgaa tttaaggctc tattattacc
     5281 tcaacaaacc accccaatat aagtttgaga ttactacaat gagcgaagca cttaaaattc
     5341 tgaacaacat ccgtactctt cgtgcgcagg caagagaatg tacacttgaa acgctggaag
     5401 aaatgctgga aaaattagaa gttgttgtta acgaacgtcg cgaagaagaa agcgcggctg
     5461 ctgctgaagt tgaagagcgc actcgtaagc tgcagcaata tcgcgaaatg ctgatcgctg
     5521 acggtattga cccgaacgaa ctgctgaata gccttgctgc cgttaaatct ggcaccaaag
     5581 ctaaacgtgc tcagcgtccg gcaaaatata gctacgttga cgaaaacggc gaaactaaaa
     5641 cctggactgg ccaaggccgt actccagctg taatcaaaaa agcaatggat gagcaaggta
     5701 aatccctcga cgatttcctg atcaagcaat aatcttttgc agattgcact tgcttaaaat
     5761 cctgccagcg gcgggatttt ttattgtccg gtttaagaca atttaa
//
LOCUS       DAJSKJ010000202         5788 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000202 DAJSKJ010000000
VERSION     DAJSKJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.206"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..819
                     /locus_tag="ON345_003489"
     CDS             <1..819
                     /locus_tag="ON345_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR3073492.1"
                     /translation="LSNALQSQIITDNHFLHHPKVDSELTRKHERARMDTENIYLPPL
                     ARGNNHNYDGKSVVEIRKLDISKESWPFNYVTETCRESDGITTTGRMLYRNLKITSAL
                     DDIYGGICKKAHAATELAEGLRLNLFMKSPSYPVENYTVHEITLGPGCNVPGYAGTTI
                     GYISTLPASQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGNDPII
                     GIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(960..1733)
                     /gene="uxuR"
                     /locus_tag="ON345_003490"
     CDS             complement(960..1733)
                     /gene="uxuR"
                     /locus_tag="ON345_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR3073493.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1948..3408)
                     /locus_tag="ON345_003491"
     CDS             complement(1948..3408)
                     /locus_tag="ON345_003491"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR3073494.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3489..4673)
                     /gene="uxuA"
                     /locus_tag="ON345_003492"
     CDS             complement(3489..4673)
                     /gene="uxuA"
                     /locus_tag="ON345_003492"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3073495.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5125..5217
                     /locus_tag="ON345_003493"
                     /pseudo
     CDS             5125..5217
                     /locus_tag="ON345_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5460..>5788)
                     /gene="fimH"
                     /locus_tag="ON345_003494"
     CDS             complement(5460..>5788)
                     /gene="fimH"
                     /locus_tag="ON345_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR3073496.1"
                     /translation="LPDYPGSVPIPLTVYCAKSQNLGYYFSGTTVDAGNSIFTNTASF
                     SPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTANYARTGGQVTAGNVQSIIG
                     VTFVYQ"
BASE COUNT         1400 a         1509 c         1459 g         1420 t
ORIGIN      
        1 ttaagcaatg cccttcagag tcaaattatt acagataatc acttccttca tcatccaaaa
       61 gtcgacagtg agttaacacg taaacatgaa cgtgcacgca tggatacgga aaatatctat
      121 cttccgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      181 agaaaacttg atattagtaa agagtcctgg ccatttaatt atgtgacaga aacatgcaga
      241 gaatccgatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      301 gctttagacg atatatatgg tggtatatgt aaaaaggcgc atgctgccac agagttagca
      361 gaaggattac ggctaaattt atttatgaag agcccttcct atcctgttga gaactataca
      421 gttcatgaaa taacattagg ccctggttgc aatgttccag gttacgccgg aaccacgata
      481 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaagg
      541 attgatatct atattgatca gataatgact gtctcagggg tggcaaattc ttccggattc
      601 gcccttgccg cattacttaa tgctaatatt gagttgggta atgacccaat aattggtata
      661 gaagcctatc ccggtaccgc ggagatccat gcaaaaatgg gatataaagt tatcccgggt
      721 gatgaaaatg caccactcaa acgtatgact ctgcaaccgt catctcttcc agaactgttt
      781 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
      841 aacattcagg ttatacagat agctaaacaa taccctgtaa tttacaaagt aatactcatt
      901 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
      961 caggcgtcga ctttatccag cggccatgaa tcaaacagat agccatcaaa ataaatatcg
     1021 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1081 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1141 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1201 ttgttttccc gccactgcca ggactgacgg aacagctcca ccagcatgct gttatgcgtt
     1261 gcttcggcaa tagcgagatg gaactgcatg tcaccgcttt cgctgctgcc cggcgcactg
     1321 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1381 gtagcctgca aagcggcaaa ctcggcgatg ttgctctcca ataactgccg cgcctgtaac
     1441 agctcaaaag gaccggcatc gttgcagacg ttggcatccg gactgtctgt gttctggctg
     1501 cctgagttat caagaacata gataccggca ccccggcgta cttccaccag ccctttgatc
     1561 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     1621 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     1681 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tggcagattt catggtgttg
     1741 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     1801 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     1861 tggcacgtat aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     1921 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     1981 ttcgcacagc tgctgatatg ccgccgtcac tgcgccaaca aagtcggcat tctgcggcag
     2041 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2101 ctgcgcgttg accttctgga actccgccag catcgggtcg accacgtcaa tggcattacc
     2161 ctgctcatcc acgccctggg tgtaacgcat ccagccagcc acgcccagcg ccaggtgacg
     2221 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agcagacgct gcggtaactt
     2281 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2341 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2401 cgcttgttcc tgcatcatca aggagaaggc cgctttgcga taatccgggt tagtcatggt
     2461 gtcggcaatg gtttcatagc cgccgaggta accgaggtat gccaggaaag agtggctgcc
     2521 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc
     2581 cactttatcc cagtccgggc gaccgttaac gaagttatct tcaatcaccc actgacggaa
     2641 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgcaa
     2701 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     2761 ttcttcaatc catgccgcca gctgcgggtc acgcgcctga gccagtccca gtaccgcgac
     2821 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa acgctttcag
     2881 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     2941 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3001 cgcatcagcg cagtagcctt tttccgtgac tgtcagagag acaatcgccg tttgcggacg
     3061 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc gggtgcagcg cttctttcat
     3121 cgaaccgata attttcagct cggtgctctc tgcgcctttt tccgccacgg tgtacagcag
     3181 ttgctgtttt ttcaggtttt cgatcagcac gcggtcgttg cctggcatca ggttaacttc
     3241 gcagataccc cagtcgctgt cggtgctttc cagcagatga tgggtgtaca gcgcctggtg
     3301 cgcacggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3361 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3421 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3481 ggtggatatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3541 gccaggcctt tcagacgacc aattgcggag taacctgggt tggttttctt cttcaggtcg
     3601 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     3661 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     3721 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     3781 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     3841 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctatt tacggtatca
     3901 accatccact gcatatcttc aatggtagaa acaatgcgcg gcaggccgag gattgggcgc
     3961 ggcggatcgt ccgggtgaac agccatacgc acgccaactt cttcggcaac cggaataatc
     4021 gccttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4081 tccaggtgct tacggaactg gtcgagggtg tacccttctt ccgcgcccgg cagaccggca
     4141 atgatgttac gggtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4201 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcac ctgggcgttt caggatatgc
     4261 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4321 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4381 cgaatgccgc actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4441 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4501 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4561 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     4621 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     4681 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accctggagt tgtttaggta
     4741 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     4801 ctaccttttg gtaagacatg tcaacccaaa atctgaaatt ggttaaccac atcacaagaa
     4861 tttcactctt atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     4921 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     4981 aacaacggtt caaccaggta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5041 tattcggcat tggtctgatg ctggtactga caaagaccgc aggttcagac acattcgctg
     5101 cattagcaat gcctcttaga taattttgac ctgtcagtaa aagacacgtt gaaaacctgg
     5161 ggtctgctgg agctggtcaa ctccgtggtt gggctgatta ttagcaaggt agcgtaaaag
     5221 aacaccagag cctgccaatg gcaggctctg actgatgaca aagtctaaaa atgtgcccgg
     5281 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agcaaagtgc
     5341 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     5401 gcaggttttt agcttcaggt aatattgcgt aactgcatta gcaatgccct gtgatttctt
     5461 tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt cacctgccct
     5521 ccggtacgtg cgtaatttgc cgttaatccc agacttaccg ccgaagtccc tactgctcct
     5581 aacgataccg tgttattcgc tggaataatc gtaccgttgc gcgtcaactg tacgccgacg
     5641 ccctgcgctg gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc atctacggtt
     5701 gtgccggaga agtaataccc cagattttgg cttttcgcac aataaacggt aagaggaatt
     5761 ggcactgaac cagggtagtc cggcagag
//
LOCUS       DAJSKJ010000203         5727 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000203 DAJSKJ010000000
VERSION     DAJSKJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.207"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(81..833)
                     /gene="yecC"
                     /locus_tag="ON345_003495"
     CDS             complement(81..833)
                     /gene="yecC"
                     /locus_tag="ON345_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR3073497.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPETGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(830..1498)
                     /gene="tcyL"
                     /locus_tag="ON345_003496"
     CDS             complement(830..1498)
                     /gene="tcyL"
                     /locus_tag="ON345_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR3073498.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1513..2499)
                     /gene="dcyD"
                     /locus_tag="ON345_003497"
     CDS             complement(1513..2499)
                     /gene="dcyD"
                     /locus_tag="ON345_003497"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR3073499.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2604..3404)
                     /gene="tcyJ"
                     /locus_tag="ON345_003498"
     CDS             complement(2604..3404)
                     /gene="tcyJ"
                     /locus_tag="ON345_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3073500.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3492..4043)
                     /gene="fliZ"
                     /locus_tag="ON345_003499"
     CDS             complement(3492..4043)
                     /gene="fliZ"
                     /locus_tag="ON345_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR3073501.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4089..4808)
                     /gene="fliA"
                     /locus_tag="ON345_003500"
     CDS             complement(4089..4808)
                     /gene="fliA"
                     /locus_tag="ON345_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR3073502.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGMAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4973..>5727)
                     /locus_tag="ON345_003501"
     CDS             complement(4973..>5727)
                     /locus_tag="ON345_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR3073503.1"
                     /translation="DTKVGGTGIDATGLAKAAVSLAKDASIKYQGITFTNKGTGAFDG
                     SGNGTLTANIDGKDVTFTIDATGKDATLKTSDPVYKNSAGQFTTTKVENKAATASDLD
                     LNNAKKVGSSLVVNGADYEVSADGKTVTGLDKTMYLSKSEGGSPILVKEDAAKSLQST
                     TNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDADYATEV
                     SNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
BASE COUNT         1355 a         1522 c         1379 g         1471 t
ORIGIN      
        1 ctgatttgtt aagcagtttt atcaggcttg aaatggcgtc cagccccgac aggtgaatcg
       61 tcggggctga ttttttctta ttattgcagc agaaacttct cgaggaactg gcgggtgcga
      121 ggctgctcgg gatcggcaaa taacgctttt gcggccccct gctcgactat ccgcccctgg
      181 tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc
      241 accatcgtgc gcttttcctg cgccagctga cggatggtgt tcaggacttc gcccaccagc
      301 tctggatcca gcgccgacgt tggctcgtca aacaaaatca cctcaggacg cattgccagc
      361 gcacgtgcaa tcgcaacacg ttgctgttga ccgccagaca aacgacgtgg atagctggtt
      421 tctttaccgg ccagcccaac tttcgccaac agctcgcgag cgcgcgccgt ggcctcttct
      481 ttcggttcgc ctttgacgat caccggccct tcaatgatgt tttccagcac cgtacgatgc
      541 ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc
      601 gatttttgct gacttaatga acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt
      661 cccgtttcgg gttgttccag cagatttatg ctgcgtagca acgtggtttt gccggaacca
      721 ctcggaccga taattgccac cacttcgcca ggctttacct caaggtcgat accgtgcagc
      781 accgtctgac cgtgaaattt tttcaccagg ttcttaactt caatggcact cattttggtt
      841 ctctctcctg gcgattaagt tgattctcaa aatggttctg caacgtcgat aatactgtcg
      901 ccatgatcca gtagatgagc gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg
      961 aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg gccagcgacg
     1021 tatcctttac caggctgata aagctgttcg acagcggcgg caacgccacc cgcgccgcct
     1081 gtggcaaaat cgcgcgacgc atagtctgcc atggcgtcat accaatactg gcagctgctt
     1141 cccactgacc tttatcaata gaagaaatgg cggcgcgcag tgtttcggcg gcataggcgg
     1201 cagtgttcag cgacagacca atcattgctg acggaatggg atctaattcg ataccaaact
     1261 gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag cggtgtacca cgaaaaatag
     1321 agatataaaa acgcgccagc cagcgcacgg gccagattgg cgacaggcgc atcagcgcga
     1381 gaataaaccc gagcagtaag ccaaaaaaca tgccgccgat actgagttgc agcgtatacc
     1441 cagccccttt cagcaggaac ggcaaggaat caataaccag ttgtatactt tcttgcatga
     1501 gcgttcccgg agctaaacgt ggggatgata ggcgaacagc gcaggcgcac cgccggtatg
     1561 aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt caatcagccc
     1621 cgccatcgct tttccggtat acacaggatc aagcagaatg ccttccagcc gcgccagcaa
     1681 tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc
     1741 ccagagtata atttccgctg atgcggtcag ctccagttct ttcgcaatcg cctgttgtag
     1801 gttaaccact ttcggcaact gatcggcaac ggaacgcgac acggtcacgc caatcagttc
     1861 gctttcaggc atcaggtgtt ccagcccaac agccagtccg gcgtgagttc cggcactgcc
     1921 cgatgcgact accaccgacg aaatattaac cgccccttca cactgttgcg cgatttccag
     1981 cgcactctcc acataaccta gcgcgccaag agcattagaa ccgccaaccg gaatgacata
     2041 cgggcgaaag ccttgtgctt cgactcgcgt cgccagctct tccagttggg cattgggatc
     2101 ggtcagtgct tcgcacattt caatctgggt attgaacaga tccagcaaca agcgattgcc
     2161 gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg ccacacagtg
     2221 cagaccgagt ttcgccgcaa ctgcggcagt ctgccgcaca tggttagact ggatcgcccc
     2281 ggcagtaatc agcgtatcgg caccttcgcg cagagcatct gctgcgagaa attccagctt
     2341 acgtaattta ttgccgccca ttgccatggg ggtgacgtca tcccgtttga tgaaaatttc
     2401 ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggtgttggcg cgccgataaa
     2461 ctccaaccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtgtgttg
     2521 ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tggtgaaagc
     2581 gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa
     2641 agggcttgca gagtgccatc tttttgcatt tccgcaattg catcgttcac tgctttcagc
     2701 aggtcttcat ttcctttacg cagcgccacg ccagactcct gacgggagaa tgcttcaccg
     2761 gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag acggtcaacg
     2821 aggatcgcat cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag
     2881 gtacgcacat cgacgccctg aacattctgc cgcagccact cttcatagtt ggtgcccaga
     2941 ccgacaccca cttttttgcc tttcagatca gcagccgttt taatggtgcc ttcgttacct
     3001 tttttcacca gcgcctgaat accagaaatg gtgtacgggg tagagaaatc gtattttttc
     3061 ttgcgctcat cagaaatggt gacctgatta atcaccacat caatgcgttt agagtccagc
     3121 gacgccagca taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt
     3181 gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa
     3241 ctgaacggcg gataagtgcc ttccagccct accagcagcg tgccgcgctc tttaacttta
     3301 ttaagcagac cttcatctgc aaaactttta acgctcatgc ccgcaaccag cgctacggcc
     3361 atcacactca tcaatgcctg acgtcccaga tgtgctaatt tcatgtttac cccgaatgtt
     3421 gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat
     3481 cttattctta tttaatatat atcagaagca ggcaggctgg aggatttctg cacaagtccg
     3541 gtacacgttt gacgctgata gtgttgatac ttccgtaatg cgatgcggta attgttggtg
     3601 ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg cagcggatcg
     3661 agggaaatat tttgctcgtg tagatgattc cccaaacggc gcaaacgaac gacatattca
     3721 cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa attgcttaaa gccgataata
     3781 tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc aaaatcggca caatgccgcc
     3841 catgattttt gttccgtttg ccagccattt tcatcaagca gcgcgtccag gcgggaaatc
     3901 tcgattttat tcactatttt gccgtcgcga actaaggtaa tgcgatcgag taatttacgg
     3961 caatgcgcgc aatgagtctg gctgtgttta aagtctttaa gatagcggct taatggccgt
     4021 cttttcaggt gctgcaccat cattaagaac tcctggtagt caaaattaaa gtgcggcatt
     4081 tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatggcctg gctgtgtaac
     4141 tgactgaccc gcgattcccc gacctccagc accgcaccaa tctctttgag attcagctct
     4201 tcctgataat agagggttaa taccagtttt tcgcgctccg gcaacgtttc gatggcttcc
     4261 atcacccgct ggcgcagatt actgtccagc agttgttgta gcgggttttc tcgctgatga
     4321 tcatcagtaa ccagttcgat gctatcgccg tgctcttcgc gccactcatc gtaggagaag
     4381 agctggctgt tattggtgtc gagcaacatt tggcgataat cggcaatatc gatccctaaa
     4441 cgttccgcaa cctcagtttc cgtggcgttg cggccaagtt cctgctccag ttgccctatt
     4501 gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga
     4561 agttcatcca gcatagcgcc acgtatacgc tgcactgcgt aagttgtaaa tgccattcct
     4621 tgtagggcgt catagcgttc gacggcatta agtaacccaa tgccgcccgc ctgtagcaga
     4681 tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc
     4741 agcgggacat aacgctgcca cagcgagtgt ttatccatta caccttcagc ggtatagagt
     4801 gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc cgtttctgca
     4861 gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt taccggtaaa
     4921 ttgcggacag aaaaaacccc gccgttggcg gggtttgagc gataagtgta aattaacgca
     4981 gcagagacag tacgttctgc gtggtctggt tagcctgcgc cagaacagag gtacccgctt
     5041 gttgcaggat ctgcgcacga gacatgttag acacttcggt cgcgtagtca gcatcttcga
     5101 tacggctacg ggcagaagac aggttgttta cggtgttgcc aaggttggtg atagcagagt
     5161 cgaaacggtt ttgtactgca ccgaggtcag aacgcagatt gtcaacttta gccaatgcct
     5221 tgtcgatggt ttcgagcggg ttggtagtag attgcaacga ttttgctgca tcttctttta
     5281 ccagaatcgg gctaccacct tctgatttgc tcagatacat agttttgtca agcccagtta
     5341 ctgtcttacc atcagcgcta acttcataat cagcgccatt tacaactaaa gaactaccca
     5401 cttttttagc gttatttaag tccagatccg atgctgtagc ggctttgttt tcaaccttag
     5461 ttgtagtgaa ctgacctgca ctatttttgt aaacaggatc agacgttttt aatgttgcgt
     5521 ccttccctgt cgcatcaata gtaaaggtta catctttgcc atcaatatta gcggttagag
     5581 tgccgttacc actgccatca aatgcaccag tgcctttgtt ggtgaaagta ataccttggt
     5641 atttaattga ggcatcttta gctaaagaaa ctgcggcttt tgcaagccca gtcgcatcaa
     5701 tacctgtacc accgactttt gtatcac
//
LOCUS       DAJSKJ010000204         5686 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000204 DAJSKJ010000000
VERSION     DAJSKJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.208"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..984)
                     /locus_tag="ON345_003502"
     CDS             complement(<1..984)
                     /locus_tag="ON345_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3073504.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQ"
     gene            complement(1069..1389)
                     /gene="chbB"
                     /locus_tag="ON345_003503"
     CDS             complement(1069..1389)
                     /gene="chbB"
                     /locus_tag="ON345_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR3073505.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(1688..2026)
                     /gene="osmE"
                     /locus_tag="ON345_003504"
     CDS             complement(1688..2026)
                     /gene="osmE"
                     /locus_tag="ON345_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR3073506.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            2228..3055
                     /gene="nadE"
                     /locus_tag="ON345_003505"
     CDS             2228..3055
                     /gene="nadE"
                     /locus_tag="ON345_003505"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR3073507.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            3285..4172
                     /gene="cho"
                     /locus_tag="ON345_003506"
     CDS             3285..4172
                     /gene="cho"
                     /locus_tag="ON345_003506"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR3073508.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(4132..4707)
                     /gene="ves"
                     /locus_tag="ON345_003507"
     CDS             complement(4132..4707)
                     /gene="ves"
                     /locus_tag="ON345_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR3073509.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFATFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(4910..5395)
                     /gene="spy"
                     /locus_tag="ON345_003508"
     CDS             complement(4910..5395)
                     /gene="spy"
                     /locus_tag="ON345_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR3073510.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKSKMPATA
                     E"
BASE COUNT         1498 a         1374 c         1348 g         1466 t
ORIGIN      
        1 ctgacgataa tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc
       61 accactgccc ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt
      121 cttaccggct gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc
      181 cagcgcccac ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat
      241 accgaagaac cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa
      301 cgatgccagt ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt
      361 attcaacgcc caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc
      421 cgagaaggaa cgcgatactg aagcaggtac gctgtcgggc agtttaatga cccaattgcg
      481 gcggacaata aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat
      541 attcgcccca cctaaccagt ttgcgccaac cgcataggcc tcaccgacac tatatggggt
      601 gacggtcata aatgctgcaa cggataacaa ccccgccgcc agcgcatcga ctttacgctc
      661 ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattattc ctaatgttcc
      721 gttatatacg ttgccgccaa tacctttcag accattaagt gtttcaatgg ttgaggcgtc
      781 gaggcgaata cctaaggaat aaaaaaacgc cccctcccca aagcttagaa aaacgttgtt
      841 aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt
      901 gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc
      961 aagcgatgca ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatattg
     1021 cggtattgat ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc
     1081 ggctttttta atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata
     1141 aagcagcgag tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa
     1201 catataagca atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc
     1261 cagtgtttcc ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg
     1321 catttttgat accagtaaag aggtagacat gcccgcagaa caaaacagat aaatgtgttt
     1381 cttttccata aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt
     1441 aacctgcttc catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa
     1501 ggagtatacg gtatagacct gagcaatagt atttctttga ccttataaat tgtttctcct
     1561 tgacctgtgg ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga
     1621 actcaggcca aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac
     1681 gaattgatta cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg
     1741 agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac
     1801 gttgacccag gatgtaggtc tggcaagtac cgcgagcatg gatcatgctc acttcagacg
     1861 aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca
     1921 ccacaggctg tacaaactgg tctttggtac gatcataagc cgtacaaccc gccagcatgg
     1981 ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg
     2041 tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc ccgtgaacga
     2101 aacggctccg ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc
     2161 gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag
     2221 gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa
     2281 tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc aaacttatcc
     2341 gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa
     2401 gctgtgccag atggcgatta atgagctgcg ccaggaaact ggcaacgaat cactgcaatt
     2461 tattgccgta cgcctgccct atggtgttca ggctgacgaa caagattgcc aggatgccat
     2521 tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggctag
     2581 cgagcaggca ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa
     2641 agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt
     2701 gggcaccgat catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg
     2761 tacagacatt aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt tactggcggc
     2821 attaggttgc ccggaacacc tttataagaa agcgccaacg gccgatctgg aagatgatcg
     2881 cccttctctg ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct
     2941 ggaagggaaa aacctacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac
     3001 cgaacataaa cgccgtccac caattaacgt tttcgatgat ttctggaaaa agtaataatt
     3061 tgcacctatt ggattgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc
     3121 cttattcgac ctacaaaact atgcaaattc aatacattgc aggagtcgaa taggcctgac
     3181 aggcgtagca cgtcagacgg tgtaaccttt gtcatcaacc cgcctctttt ttaatcgcct
     3241 cccgcctgtt acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa
     3301 cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacgtt
     3361 cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca
     3421 ccatgccgct ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttgc
     3481 gcaccccgga tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgcaccg
     3541 caggcgaaat cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt
     3601 ttaataaacg gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg
     3661 tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt
     3721 ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa caaaaacttt
     3781 gttatggcct gttgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc
     3841 taaaacgttg cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct
     3901 tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt gcggtggcac
     3961 tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg
     4021 gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc
     4081 acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac
     4141 ttgatccggc gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg
     4201 ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag
     4261 caatttatcg cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc
     4321 aaaggtagca aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgtgt
     4381 catgatgttg aaatccatcg acatctgccc tgccgtcagt ttcgctttta ccacctggtc
     4441 cgctgcaaag gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc
     4501 aaggagcatc tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga
     4561 aaactcccca ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc
     4621 cggtgggaaa gtgcaaattt cgcgcgtttc accggcagca tttcgccaca gattcaccga
     4681 cattttacgc atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc
     4741 gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg
     4801 ctttttttac aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac
     4861 tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag
     4921 ttgcaggcat tttacttttt gccgctggac gttctgtcag acgcttctca aaattagcat
     4981 taaattgctt tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg gtttccatgt
     5041 gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct tcagcttttg
     5101 ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcggcgttct tccagcggcg
     5161 gacgtttcat ctggtcacgc tggcctttca tgatttcgcg gatctgctgt ttctgcgcgt
     5221 cggtcaggtt caggtctttg aacatcatgt cctgatgcgg accgaacttg cctttgtggt
     5281 gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt gtctgcggca tgggccaggt
     5341 tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta cgcatattct
     5401 atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt gttgacgaga
     5461 ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa
     5521 aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag tgatgagaga
     5581 atattacaat acgatgattt tgcagatatt atgaagaact ataccggatg actggtgata
     5641 aataaagcaa ataaccagga ttaatctgta ttaatttata agaaag
//
LOCUS       DAJSKJ010000205         5523 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000205 DAJSKJ010000000
VERSION     DAJSKJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.209"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(2..1114)
                     /gene="ydiK"
                     /locus_tag="ON345_003509"
     CDS             complement(2..1114)
                     /gene="ydiK"
                     /locus_tag="ON345_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR3073511.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1503..4559
                     /locus_tag="ON345_003510"
     CDS             1503..4559
                     /locus_tag="ON345_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3073512.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4556..4966
                     /gene="menI"
                     /locus_tag="ON345_003511"
     CDS             4556..4966
                     /gene="menI"
                     /locus_tag="ON345_003511"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR3073513.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5066..5254
                     /locus_tag="ON345_003512"
     CDS             5066..5254
                     /locus_tag="ON345_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR3073514.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
BASE COUNT         1345 a         1421 c         1472 g         1285 t
ORIGIN      
        1 attacttatt cggtttctcg atttcgccca gttcttcgag aatttcttcc ggttggtccg
       61 tcggcggcgg gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa
      121 ccggaccaat aaacagaccg atcatcccga aagcaatcaa accaccaata acaccagaga
      181 ggatcaggat cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat
      241 ccagcgtgcc aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccag
      301 tccagtagag ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc
      361 aggagaggat cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc
      421 cgataccgcc aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc
      481 ggatagcctg tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg
      541 caaaatggcg aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga
      601 gaagcatcag cgcacaatgc accataaagc gcccgatatg cgccgcttgc ccaacgaacc
      661 aggtggtggt ggtgccaata taagggcgga ctttcgccat gatcgccgtc ccccccatat
      721 ccagcaagtt gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc
      781 acgccagatc gggtaacgtc atgtcaccgc tggaaatggc tttaattagc gggccgctgc
      841 cgtcgacgat actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca
      901 gcgtcatcac cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca
      961 ataccggcca ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt
     1021 gaacaatcca cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt
     1081 gtgcgacatc cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt
     1141 caaatcgtcg acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag
     1201 cggtttttaa caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg
     1261 tacaacaact caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg
     1321 cggctacgtt cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg
     1381 ccgccttcct gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt
     1441 gcagccgcct gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata
     1501 taatgattcc acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc
     1561 aagagctgga gcaacaaggt tttaccggcg atacggcgac aagttatgcc gatcgtctga
     1621 caatgtcgac cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt
     1681 caaccgcaga tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga
     1741 tctttacccc ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta
     1801 ttgttgatat gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg
     1861 tgcgcgttga ggccggggtg ataaaagatc aactcaatca gtacctgaaa ccgttcggct
     1921 acttttttgc gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata
     1981 ccgatgcatc cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg
     2041 tacgcgcggt gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgaattgg
     2101 cggaaacgct gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac
     2161 gttgccgtca gcaacgccag ttaattatcg acaacttccc caaacttaac cgctttctta
     2221 ccggttacga tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca
     2281 ttctgacggg ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc
     2341 gcttgcctaa agtgcgccgt ctggtgaacg tcaaatatga ctcttttgac tctgcgctgc
     2401 gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag
     2461 tgctgaatct ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg
     2521 tgcctgacca agagatgctc gggctgaaca ttgtggaatt tgctggtgat gatgaggcgc
     2581 tgattgatga gcgggtaaat gcactctgtg cgcggttgga tgagctgatc gccagtcatc
     2641 aagcaggtgt catcggctgg caggtgtgcc gtgagctggc gggggttgaa cgtatctatg
     2701 caatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc
     2761 cgtttgctga ggatacctgc gtaccgccgg aacacctggc ggattatatt gctgaatttc
     2821 gcgcgctgct cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg
     2881 tcttgcacgt ccgtccagcg ctggatatgt gcgatcctca acaagagatt ttgatgaagc
     2941 aaatctctga tgacgtggtg gcgctgactg cgaaatacgg tggtttattg tggggcgagc
     3001 acggcaaagg ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag
     3061 aactgcgcaa agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt
     3121 gcccgccaga aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat
     3181 tcgatcggca gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg
     3241 gcaacggttt atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca
     3301 cccagaaccg gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgtt
     3361 tgttggcgga tcgcggcgtt gatccactca aactggaaca agaactgcct gaatccggcg
     3421 tcagtttgcg gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat
     3481 atgacttctc acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcgtgtt
     3541 cgacccagtg ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct
     3601 atcacacccg ttatttacgc ccgctgcgcg accacctcgt cgctacggtc gagagctacg
     3661 cgccgctgat ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc
     3721 gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc gctgctgtcg gtcccctcgc
     3781 tacaacaaca aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaatctc
     3841 tcaatgcaga gcagaaagcg cgcacagtgt tggtggtgca ggaccccttt accagctatt
     3901 acgatgcgca agtggtggcg gattttgtcc gtctggtcga aaaattaggt ttccagcctg
     3961 tgttactgcc attttcgcca aatggcaaag cccagcatat taaaggtttt cttaatcgtt
     4021 ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg tatggcgaag ctgggtatgc
     4081 caatggtggg cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc
     4141 tgggcgagga gcgtggcgag tttaacgtct tactggcgaa tgaatggctg gcaagcgcac
     4201 ttgagtcaca gccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta
     4261 ccgaagttac cgccttgccg ggtgcgccag cacaatgggc cgcgatattt gcccgttttg
     4321 gcgcgaaact ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg
     4381 aagcgaaaaa ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc
     4441 agcgactgcc gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac
     4501 gggttgaagg cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat
     4561 atggaaacgg aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg
     4621 gttcctggat attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt
     4681 agactcgcgg acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc
     4741 cgaaagtatc ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg
     4801 tctggaaatc aatgctaacc acgtccgctc ggcacgagaa gggcgggtgc gcggcgtatg
     4861 caaaccgttg catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa
     4921 agggcgtttg tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa
     4981 aagtgtgacg catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca
     5041 ggtcttgtct aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat
     5101 tgaattttta cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa
     5161 acaactagaa cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga
     5221 aatctacttt gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc
     5281 aaatcgacct ggtttaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac
     5341 cgcctgatat tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct
     5401 ttatgagatt attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag
     5461 cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt
     5521 ctg
//
LOCUS       DAJSKJ010000206         5490 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000206 DAJSKJ010000000
VERSION     DAJSKJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.210"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..642
                     /locus_tag="ON345_003513"
     CDS             <1..642
                     /locus_tag="ON345_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3073515.1"
                     /translation="NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTI
                     SSDTPVDYSQPMADVYIRGDITAPQSCEINNLQPVYFDFKEIPAADFSSVVGSAVTTH
                     KITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKV
                     NGGELPTDMGKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            890..1930
                     /gene="pstS"
                     /locus_tag="ON345_003514"
     CDS             890..1930
                     /gene="pstS"
                     /locus_tag="ON345_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR3073516.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLQFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2017..2976
                     /gene="pstC"
                     /locus_tag="ON345_003515"
     CDS             2017..2976
                     /gene="pstC"
                     /locus_tag="ON345_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR3073517.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2976..3866
                     /gene="pstA"
                     /locus_tag="ON345_003516"
     CDS             2976..3866
                     /gene="pstA"
                     /locus_tag="ON345_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR3073518.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3957..4730
                     /gene="pstB"
                     /locus_tag="ON345_003517"
     CDS             3957..4730
                     /gene="pstB"
                     /locus_tag="ON345_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR3073519.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4745..5470
                     /gene="phoU"
                     /locus_tag="ON345_003518"
     CDS             4745..5470
                     /gene="phoU"
                     /locus_tag="ON345_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR3073520.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1336 a         1333 c         1464 g         1357 t
ORIGIN      
        1 aattgcaacc gcattgaacc tttaagtata tctagtgggg ctaaagcgat agtatatttt
       61 tatatcaaga agacatttgc aggaaaattg attattcctg aaacaaagat agtgacattg
      121 tatggaacaa ttagcagtga caccccggtg gattactcac agccgatggc tgatgtttat
      181 attcggggcg atattacagc tccgcaaagt tgcgaaataa ataatttaca gccagtttat
      241 tttgatttta aagaaatacc agctgcagat ttttcatctg ttgttggaag tgcggtaaca
      301 acacataaaa ttactaaaac ggtcactatt gagtgtgaaa acctggggat actgaatacc
      361 gatgatatca gtacgtcttt ttatgctacc gaacccaata cggacaattc aatggtcgta
      421 acttcaaact ctaacgtggg gataaaaatt tacgacaaaa ataataagga aatcaaagtg
      481 aacggtggtg agttgccaac agacatgggt aaatcaacag tgtatggtga aaaatcaggt
      541 agtgtaactt tttcagctgc tcctgcgagc cttactggag ctcgcccagc cccggggcaa
      601 tttactgcaa cagcgacgat aactgttgag attgtgcgct aataggtctg acaacgattg
      661 ttcatcacat cgttgtcatc tcacccattt ctttatcact tttaaatgca tcatgacaaa
      721 aagatgacat gtgggatttt catcttcaca tttaaaatca gtaagttatt tggtaattcc
      781 tttctgtcat aaaaatgtca tattccttac atataactgt cacctgtttg tcctattttg
      841 cttctcgtag ccaacaaaca atgctttatg aatcctccca ggagacatta tgaaagttat
      901 gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg atgagcgctt tctctgtgtt
      961 tgcagaagca agcctgacag gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg
     1021 ggctgacact taccagaaag aaaccggtaa taaagttaac taccagggta tcggttcttc
     1081 cggtggcgta aaacagatta tcgctaatac cgttgatttt ggtgcctctg acgcgccgct
     1141 gtctgacgaa aaactggctc aggaaggtct gttccagttc ccgaccgtga ttggcggcgt
     1201 ggtgctggcg gttaacattc cagggctgaa gtctggcgaa ctggtgctgg atggtaaaac
     1261 cctcggcgac atctacctgg gcaaaatcaa gaagtgggat gatgaagcca tcgccaaact
     1321 gaatccgggt ctgaaactgc cttcacaaaa cattgctgta gtacgccgcg cagatggctc
     1381 cgggacttcc ttcgtcttca ccagctacct ggcgaaagtg aacgaagagt ggaaaaacaa
     1441 cgttggtact ggctctaccg taaaatggcc gatcggtctg ggcggtaaag gtaacgacgg
     1501 tatcgccgcg ttcgttcagc gtctgccggg tgcaattggt tatgttgaat atgcttacgc
     1561 gaagcagaac aacctggcgt acaccaaact gatctccgct gatggtaaac cggttagtcc
     1621 gaccgaagaa aacttcgcta atgcagcaaa aggtgcagac tggagcaaaa ccttcgctca
     1681 ggatctgacc aaccagaaag gcgaagatgc atggcctatt acctctacca cgttcattct
     1741 gatccacaaa gatcagaaga aaccagaaca aggcacagaa gtgctgcaat tcttcgactg
     1801 ggcgtacaaa accggggcta aacaggcgaa cgacctggat tacgccagcc tgccggatag
     1861 tgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt aaagacagta gcggtaagcc
     1921 gctgtactaa taaaactcca ggccgggtac ggtgttttac gccgcatccg gcattacaaa
     1981 atgactttgt aaacgcgttt aactgaagag taacttatgg ctgcaaccaa gcctgctttt
     2041 aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg
     2101 attgtgctat tgatgttggg tggcattatt gtctctctga tcatctcctc ctggccgagc
     2161 attcagaaat ttggtctggc tttcctatgg accaaagagt gggatgcacc gaacgatatc
     2221 tacggggcgc tggtgccgat ctacggtacg ttggtgactt cgtttatcgc gctgctgatc
     2281 gccgtcccgg tgagtttcgg tatcgccctg ttcctgactg agcttgcgcc tggctggctg
     2341 aaacgcccgc tgggtatcgc cattgagctg ctggcagcca ttccaagtat cgtttacggc
     2401 atgtggggcc tgtttatctt tgcgccgctg ttcgccgttt actttcagga gccggtcggc
     2461 aatatcatgt cgaatatccc gattgttggc gcgctgttct ctggccccgc atttggtatc
     2521 ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta ttccgtacat tgcggcggta
     2581 atgcgtgatg tgttcgaaca aaccccggtg atgatgaaag agtcggccta cggtattggc
     2641 tgcaccacct gggaagttat ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc
     2701 ggcggcatca tgctggggct gggccgcgcg ctcggtgaaa ccatggcggt gacctttatc
     2761 atcggtaaca cctaccagct cgacagcgcc tcgctgtata tgccgggcaa cagtatcacc
     2821 tctgcgctgg cgaacgaatt tgcggaagcg gaatccggtc tgcacgttgc cgcactgatg
     2881 gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg
     2941 attatgcgcc tggctaagaa tgagggggca cgctaatggc tatggttgaa atgcaaacca
     3001 ctgcggcgct ggctgaatct cgccgcaaaa tgcaggcgcg tcgccgcctc aaaaaccgta
     3061 ttgcgctgac gctctcgatg gcgacgatgg ccttcggcct gttctggctg atctggattt
     3121 taatgtccac catcactcgc ggtatcgacg gtatgtcgct ggcgctgttc actgaaatga
     3181 cgccgccgcc caatacggaa ggtggtggtc tggcgaacgc cctggcgggt agcggactgt
     3241 taattttgtg ggccacggta ttcggtacgc cgctgggcat tatggcgggg atttatctgg
     3301 cggaatatgg tcgtaaatcc tggctggcag aagtgattcg cttcattaac gacattctgc
     3361 tctccgcgcc gtcgattgtg gttggtctgt ttgtttacac catcgtggtg gcgcagatgg
     3421 agcacttctc cggctgggcg ggcgtgattg ccctggcgct gttgcaggtg ccgattgtta
     3481 tccgcaccac cgagaacatg ctgaaactgg tgccggacag tctgcgtgaa gcggcttatg
     3541 cgctgggtac accgaagtgg aagatgatct ccgcgattac gctgaaagcg tcggtgtccg
     3601 ggatcatgac cggtatcttg ctggcgattg cccgtattgc tggtgaaacc gcgccgctgc
     3661 tgtttaccgc gctctccaac cagttctgga gcacggacat gatgcagccg atcgccaacc
     3721 tgccggtgac gatctttaag tttgcgatga gtccgtttgc ggaatggcag caactggcct
     3781 gggccggggt attgatcatt accctgtgcg tactgctgct gaacattctg gcgcgcgttg
     3841 tttttgcgaa gaataaacac ggttaacact ttgccggatg cggcgtgaac gcctgatccg
     3901 gcctacggta agcctgatta gcgaagtgca tcaggcacga tgaggaaaag attgcaatga
     3961 gtatggttga aactgccccg agtaaaattc aggttcgtaa tttgaacttc tactacggca
     4021 aattccatgc cctgaaaaac atcaacctgg atatcgctaa aaaccaggta acggcgttta
     4081 tcgggccgtc cggctgcggt aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac
     4141 tgtacccgga gcagcgtgcg gaaggtgaaa ttctgcttga tggcgacaac atcctgacca
     4201 actctcagga tatcgcactg ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc
     4261 cgtttccgat gtccatctac gacaacatcg cttttggcgt tcgtctgttt gagaagctct
     4321 cccgtgccga catggacgag cgcgtgcagt gggcattgac caaagccgca ttgtggaacg
     4381 aaaccaaaga taaattgcac cagagcggtt actctctctc tggtggtcag caacagcgtc
     4441 tgtgtattgc gcgtggtatc gccattcgcc cggaagtgct gctgctcgac gaaccgtgtt
     4501 cggcgctcga ccctatctct accgggcgta ttgaagagct gatcaccgaa ctgaagcagg
     4561 attacaccgt ggtgatcgtc acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca
     4621 cggcgtttat gtacctgggc gaattgattg agttcagcaa cacggacgat ctgttcacca
     4681 agccagcgaa gaaacaaaca gaagactaca tcaccggtcg ttacggttga ttcaggagtg
     4741 cgttatggac agtctcaatc ttaataaaca tatttccggc cagttcaacg ccgaactgga
     4801 aagtatccgc acgcaggtga tgaccatggg cggcatggtg gagcagcagc tttctgatgc
     4861 aatcaccgcg atgcataacc aggacagcga tctggcgaag cgcgtgatcg aaggcgacaa
     4921 gaacgtcaac atgatggaag tggcgatcga tgaagcctgc gtgcgcatta tcgccaaacg
     4981 tcagccgacg gcgagcgacc tgcgactggt tatggtgatc agtaaaacca ttgccgagct
     5041 ggagcgtatt ggcgacgtgg cggacaaaat ctgccgtact gcgctggaga aattctccca
     5101 gcagcatcag ccgttgctgg taagtctgga gtcgctgggc cgtcatacca tccagatgct
     5161 gcacgacgtg ctggacgcgt tcgcgcggat ggacattgac gaagcggtac gtatttatcg
     5221 tgaagataaa aaagtcgatc aggaatacga aggtattgtt cgtcaactga tgacctacat
     5281 gatggaagat tcgcgtacca ttccgagcgt acttactgcg ctgttctgcg cgcgttctat
     5341 cgaacgtatt ggcgaccgct gccagaatat ttgtgagttt atcttctact acgtgaaggg
     5401 gcaggatttc cgtcacgtcg gtggcgatga gctggataaa ctgctggcgg ggaaagatag
     5461 cgacaaataa ttcaccagac aaatcccaat
//
LOCUS       DAJSKJ010000207         5448 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000207 DAJSKJ010000000
VERSION     DAJSKJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.211"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..884
                     /gene="adeQ"
                     /locus_tag="ON345_003519"
                     /gene_synonym="yicO"
     CDS             <1..884
                     /gene="adeQ"
                     /locus_tag="ON345_003519"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR3073521.1"
                     /translation="GIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFI
                     ITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLE
                     LAGIIFSFMLINLFDSSGTLIGVTDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGT
                     SSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVL
                     MTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNL
                     CVVVVAALFALKIILVD"
     gene            937..1389
                     /locus_tag="ON345_003520"
     CDS             937..1389
                     /locus_tag="ON345_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR3073522.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1600..2790
                     /gene="nepI"
                     /locus_tag="ON345_003521"
     CDS             1600..2790
                     /gene="nepI"
                     /locus_tag="ON345_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR3073523.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2831..3124)
                     /locus_tag="ON345_003522"
     CDS             complement(2831..3124)
                     /locus_tag="ON345_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073524.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLPKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3346..4164
                     /gene="nlpA"
                     /locus_tag="ON345_003523"
     CDS             3346..4164
                     /gene="nlpA"
                     /locus_tag="ON345_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR3073525.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAMGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4168..5091)
                     /locus_tag="ON345_003524"
     CDS             complement(4168..5091)
                     /locus_tag="ON345_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR3073526.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLSVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5201..>5448)
                     /locus_tag="ON345_003525"
     CDS             complement(5201..>5448)
                     /locus_tag="ON345_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073527.1"
                     /translation="ILNAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIT
                     GSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1311 a         1236 c         1398 g         1503 t
ORIGIN      
        1 gcggaattgg attatttatc gccttaatgg gattaaaaaa tactggcgtt attgtcgcca
       61 ataaagacac gctggtgatg attggcgatt taagttctca cggcgtgttg ttaggtattt
      121 tagggttttt tattataacc gtgttgtcat cacgtcattt tcatgccgcg gtgctggttt
      181 ctattgtggt gacgtcttgc tgtggattat ttttcggtga tgttcatttt agcggcgtct
      241 attccattcc gcctgatatt agcggcgtca ttggtgaagt agatttgagc ggcgcgttaa
      301 cacttgaact cgccggtatc attttctcct ttatgctgat caacctattt gattcatcag
      361 gaacattaat tggtgtaact gataaagcgg gcttaataga tagtaacggt aaattcccca
      421 atatgaataa ggcgctgtat gttgatagcg tcagttcggt ggcgggggcg tttatcggca
      481 cctcgtctgt tactgcctat attgaaagta cttctggtgt ggcagtcggt ggtcgcacgg
      541 ggctgactgc ggttgtggtt ggcgttatgt tcctgttagt tatgttcttc tcaccgctgg
      601 tggcgatggt tcctccttac gcaaccgccg gagcgttaat ctttgttggc gtgctgatga
      661 cttcgagcct ggcgcgcgtt aactgggatg attttaccga atcggtgcct gcgtttatta
      721 ccacggtgat gatgcccttt actttctcga tcaccgaagg gattgcactc ggctttatgt
      781 cgtactgcat catgaaagta tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg
      841 tggtcgcagc tctgtttgca ctgaagatta ttctggtgga ttagtaactg gcgagggttt
      901 ccagatatca tgagttctga ttacgcagga gaactcatga tctggataat gctcgccacg
      961 ctggcggtag tgtttgtggt tggttttcgg gtgctgacat ccggggccag aaaagcgatt
     1021 cgccgtctca gcgatcggct gaacatcgat gtcgtacccg tggagtcgat ggtcgatcaa
     1081 atgggaaagt cagctggtga cgaattttta cgttatttgc atcgtccgga tgagtcgcac
     1141 ctgcaaaacg ccgcgcaggt gttgctcatc tggcaaattg tcattgtcga tggtagcgaa
     1201 cagaacctgc tgcaatggca tcggatttta caaaaagctc gcctcgccgc gccgattacc
     1261 gacgctcagg tcaggctggc gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat
     1321 attaatgctt ttcagatgcg ctataacgcg ttctttcagc ctgccgaggg cgttcactgg
     1381 ttgcattgaa accgtttcgt cttatcagcc tacagaccgc gcctgacctg tcgggtggat
     1441 aagacgttca cgccgcatcc ggtaatcttt gccctgttcc gtttcgatct taactccagc
     1501 tcaagcaaca ccgtaaaagt tgcaaaaacg ttaaatgcgt cacacatttc aatgttaaag
     1561 tggtcggctt ttcccctgaa acatgccacg ggtaacacca tgagtgaatt tattgccgaa
     1621 aaccgcggcg cggatgccat cacccgaccg aactggtcag ccgttttctc ggtggcgttt
     1681 tgtgtcgcct gtctgattat cgttgagttt ttgcccgtca gtttgttgac gccaatggcc
     1741 caggatttag gcatttcgga aggggttgcc gggcaatcgg tgaccgtgac cgcctttgtg
     1801 gcaatgtttg ccagtttgtt tattacccag acaattcagg ctactgaccg ccgctacgtt
     1861 gttattttgt ttgccgtttt gctgacgctc tcctgcttgc tggtttcctt tgctaactca
     1921 ttcagtttgc ttttaatcgg tcgtgcctgt ctggggctgg cgctgggcgg gttctgggcg
     1981 atgtcggcgt cgctgaccat gcgtctggtg ccgccgcgta cggtgccgaa ggcgctgtcg
     2041 gtgatcttcg gcgcggtttc tattgcgctg gtgattgccg cgccgttggg cagtttttta
     2101 ggcgagctta tcggttggcg caatgtcttt aatgcggcgg cggtgatggg cgtgctgtgt
     2161 attttctgga ttatcaaatc attgccttca ctgccaggcg aaccctcgca tcagaaacaa
     2221 aatactttcc gcttattaca acgtccgggt gtgatggcag ggatgatcgc catcttcatg
     2281 tctttcgccg ggcagtttgc tttcttcacg tatattcgcc cggtgtatat gaacctagcg
     2341 ggattcggcg tggatggctt aacgctggtg ctgttgagtt ttggtatcgc cagctttatt
     2401 ggtacgtcgc tttcgtcgtt cattcttaaa cgttcggtaa aactggcctt agcaggcgcg
     2461 ccgttaatac tggctgtgag tgcgttggta ctgacgttgt ggggaagcga taaaatcgtt
     2521 gctaccggcg tggcgattat ctgggggcta acttttgcat tggttcccgt cggctggtca
     2581 acgtggatca cccgctcgct ggccgatcag gcagaaaaag ccgggtctat tcaggtggcg
     2641 gttattcagc ttgctaatac ctgtggcgcg gcaatcggcg gttatgcgct ggataatatt
     2701 ggtctgactt cgccgttgat gttgtccggc acattgatgt tgctgactgc attgttggtt
     2761 actgcaaagg tgaaaatgaa gaaatcctga tatcaggtaa agaagcgttg gtcagtagtt
     2821 gttgctgttc ttacatatcc gggcattcta ccttacccaa cgcttcccaa taattgctct
     2881 gattattacg ttccatcgcc acgatggcct cgcgtatatg ttgtttattg ttttcatctg
     2941 acaacattaa cttttcccac gcttcatctg ataatgacgc ctgtggcgtg caaattttag
     3001 gtaaatcttg taataccgtg gttttctctt ttgcggactg cagtgagcta aaaggcgact
     3061 cggcgtatac gatgcctggc atagcgaaaa aagtgaaaat aagcagtagt gtcgttggtt
     3121 tcatggattc aaccttttac aaatgtgtat cgaatgaagc ataagtgtag tttgctttca
     3181 tcgctctcat catgctgtac gaaacgcgct tgcgtggtca gtaagaagtg ccagacttta
     3241 tattccactt ttattccttt ttattcttat cgatagcgtt tcgtttttta aaccgcagcg
     3301 accttaccgc tatagtcagg taatcattaa taaaaggata aaaaaatgaa actgacaaca
     3361 catcatctac ggacaggggc cgcattattg ctggccggaa ttctgctggc aggttgcgac
     3421 cagagtagca gcgatgcaaa acacattaaa gttggcgtta taaatggcgc agaacaagat
     3481 gtcgcggaag tcgctaaaaa ggtggcaaaa gagaaatatg gcctcgatgt tgaactggta
     3541 ggatttagcg gttcgttgct accgaacgat gcgacaaatc atggcgaact tgatgcgaat
     3601 gtcttccagc atcgaccctt tcttgaacaa gataatcagg cgcatggtta taaactggta
     3661 gcgatgggaa atacgtttgt cttccctatg gcgggttatt ccaaaaaaat cacaaccgtg
     3721 gcgcaaataa aagaaggcgc gacagtggcg atccccaacg acccgaccaa ccttggtcgc
     3781 gcacttttac tgttgcaaaa agagaagcta attactctga aagagggaaa agggttatta
     3841 cctaccgcgc tggatataac cgataatcct cgtcatttgc agattatgga actcgagggg
     3901 gcgcagcttc cgcgcgtact ggatgatccc aaagttgatg tagcgattat cagcaccact
     3961 tacattcagc agacagggct ttctccggtg cacgacagcg tatttattga agataagaat
     4021 tcgccgtatg tgaatatttt ggtggcacgg gaagataata agaatgcaga aaacgtgaag
     4081 gaatttctgc aatcttatca atcacccgaa gtcgctaaag cggcagaaac catttttaac
     4141 ggtggcgcgg tgcctggctg gtaattctca ctcatgccgc gccggacgat ttatttttct
     4201 ggcgcggcga cgggaatcca ttgaaatcaa aattaccgat gacagaatca gcagcgttcc
     4261 cagccagtcc ggtaatgtga acgtgatccc cagcaacagc aaagagagta gcgcgctact
     4321 taatggttct gcacagctca aaatgctggc ttttggaccg ccaattaatt gtgctccttt
     4381 caggtacaga ctaaatgtca gggacgtacc aatgacgatc aaataaaaaa acgccaaaat
     4441 caaactgccg ttaacgacaa agtttgtccc ttgtctggca taaaaaggca acagaatcag
     4501 accgccgatc agcatactcc agccgacgac agacaacgtg ccatagcggg caattagcgt
     4561 tgagggatag gtggtataga atgcagcagc aaatgccgag gcaatgcccc agaacaacgc
     4621 ggcaggagag atcgataatg acgtcggatt accgtgtgtc accaataaaa aagtcccgac
     4681 cagcgatgtc aaaatagcgc agaaaaccag aatgcccggg cgcgatttac gcaccagtga
     4741 gaaccaggcg acgataatcg tcggtgagag gaattgcagc accgttgccg tggctgcgtt
     4801 cgatttttcg atggttagca aaaaagtgag ctgtacagtt agcgcgccaa ccacggaaaa
     4861 aatcagcagg ctaatggcat ctttatgatt gttaataata gaaaagattt tatcgccatg
     4921 aacaaatgac agcgtcagta gaatcaaacc ggcgaatatc aaacgcgtca tagtcaaaaa
     4981 ctgcgacgac atctggcttt gctccatgat gtattgcgcg cagacccctg aacttcccca
     5041 caacacggcg gcaatcagaa cgttcagcat cccctttctg gtggaaccca ttttccctct
     5101 gcatactgtt ttgtcgttta tgtatgcagg catgatagca aaaatggcga ggatggctct
     5161 gtttgcgcca cgccgatttt tcttagccca gactggtgag ctaaatatct ttaataaaca
     5221 gcaggcaaat catcgcaata cccaacatcc ctatcgccag ccagaacaac gcatggtagc
     5281 tccagatttc aaccataatt ccgtcaacag agccggtgat aatccagccg acgcgtgaag
     5341 tatttgcata taacgttgtc gcagagccta ttttttcagg catcaagtcc tgaaaataaa
     5401 gcatgccgat accacagaga ataccaagga agatggcatt aagaattt
//
LOCUS       DAJSKJ010000208         5401 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000208 DAJSKJ010000000
VERSION     DAJSKJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.212"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(117..1400)
                     /gene="aroA"
                     /locus_tag="ON345_003526"
     CDS             complement(117..1400)
                     /gene="aroA"
                     /locus_tag="ON345_003526"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3073528.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1471..2559)
                     /gene="serC"
                     /locus_tag="ON345_003527"
     CDS             complement(1471..2559)
                     /gene="serC"
                     /locus_tag="ON345_003527"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR3073529.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFCDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2758..3450)
                     /locus_tag="ON345_003528"
     CDS             complement(2758..3450)
                     /locus_tag="ON345_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR3073530.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3580..5340
                     /locus_tag="ON345_003529"
     CDS             3580..5340
                     /locus_tag="ON345_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCR3073531.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1391 a         1397 c         1277 g         1336 t
ORIGIN      
        1 attggcaagc gacgctacca ttaatcaaaa tgactcacaa ggtccgaaaa aaaacgccga
       61 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
      121 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      181 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      241 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      301 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      361 gcaaacaggc gatcggtttc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      421 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      481 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      541 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      601 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      661 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      721 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      781 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      841 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      901 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      961 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
     1021 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1081 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1141 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1201 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1261 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1321 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1381 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1441 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1501 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1561 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1621 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1681 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1741 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1801 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1861 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1921 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1981 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     2041 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2101 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2161 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2221 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2281 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2341 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcaaa aatccttctc
     2401 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2461 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2521 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2581 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2641 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2701 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2761 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2821 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2881 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2941 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     3001 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3061 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3121 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3181 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3241 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3301 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3361 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3421 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3481 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3541 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3601 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3661 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3721 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3781 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3841 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3901 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3961 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     4021 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4081 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4141 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4201 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4261 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4321 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4381 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4441 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4501 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4561 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4621 ttctccggca ccacggaaga agagttcgcc acgctgatgg ctatcttcaa aaaggaagat
     4681 aaagaagttt atattgccga ttacgagcat ctgggtgtct acgcttgccg cattatcgtg
     4741 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4801 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4861 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4921 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4981 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     5041 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5101 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5161 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5221 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5281 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5341 aatcaaatag cctacgcaat gtaggcttag tgattagtct gagttatatt acggggcgtt
     5401 t
//
LOCUS       DAJSKJ010000209         5278 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000209 DAJSKJ010000000
VERSION     DAJSKJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.213"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            10..372
                     /locus_tag="ON345_003530"
                     /pseudo
     CDS             10..372
                     /locus_tag="ON345_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(341..2077)
                     /gene="aceK"
                     /locus_tag="ON345_003531"
     CDS             complement(341..2077)
                     /gene="aceK"
                     /locus_tag="ON345_003531"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR3073532.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFTSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITSSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2261..3565)
                     /gene="aceA"
                     /locus_tag="ON345_003532"
     CDS             complement(2261..3565)
                     /gene="aceA"
                     /locus_tag="ON345_003532"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR3073533.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3595..5196)
                     /gene="aceB"
                     /locus_tag="ON345_003533"
     CDS             complement(3595..5196)
                     /gene="aceB"
                     /locus_tag="ON345_003533"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR3073534.1"
                     /translation="MTEQATKTDELAFTRPYGEQEKQILTAEAVEFLIELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPTDLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLDEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1238 a         1415 c         1341 g         1284 t
ORIGIN      
        1 attcgataaa aaaatatgaa gcccctcctc ctggcaaata attctaatga atatcccggt
       61 ttgttttcag cgatacagca taaacaacaa aacgttgtag agacggttta tcttgcttta
      121 tctgaccatg cacgcctgtt tggatttacc gctgaagata ttatggattt ttggcaacac
      181 aaagcgccac aaaaatactc tgcctttgag ttggcttttg aatttggtca ccgggttatt
      241 ggtgaattaa tccttaatac attaaataag atggctgaaa gctttggctt tacggataac
      301 cctcgataca ttgcggagaa aaattatatg gaagctttac tcaaaaaagc atctccccat
      361 accgtacgct aaatctttgc ctgcgccgat acgcataaac atcttccaca tgcccttcac
      421 gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa caggtcggcg tgcatctctt
      481 caaacagcgg accaatacgc gggtcggcgc atagccagtg gcgaaactct tccgggaaaa
      541 catcgcccgg cgagacgctg taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg
      601 gcggcgggat gtcgcggaaa ttcacttccg tcatgtagca aatttcatcg taatcataaa
      661 aaaccacacg cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg ccaggaaaaa
      721 tgttagcagc ggcaagctgg cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact
      781 gctgaccttc cacttgttcc agccagatat tgagcggcac catccgccgc tcaatataaa
      841 gatggcgaat aacaatttgt tcgccgagat cggtgatttt ttccgctgct tcctggagca
      901 gtaattccat taatgccggg gaaatatgcc gcttctccag cacaaagttt tcaaactcct
      961 gggtgtccgc cattcggccc acgcgatcgt gctctttcac cagttgatag caggcacgaa
     1021 cgtgagcggc agacatctct ttctgcggcg cgaacttgtc tttgatgact ttaaatacac
     1081 ggtcaaagcc cggcagcgtg aacaccaaca tcaccatgcc acgaataccc ggcgcttcaa
     1141 tgaactgctc attacagccc tgtagataaa cgagatattc gcggtagctt tcggttttgg
     1201 cgtgcttctg gcagccgata gccatataca attcagcggt ggttttaccc ggcagaattt
     1261 cccgtagcca ctcgaccaac gctgcgggca gcggcgcgta aaccataaaa taagaacgcg
     1321 cgaagccaaa gacgatgctc gcttcggcgg tagtcgtcag gcaggtatca ataaataact
     1381 cgccatcgtc cgtctggtgg atcggcagca aaaagggcaa tgtgccggaa gacgtgatca
     1441 gtttgcccac cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat
     1501 gactttccgc gaggttgtct gtccccagcg tttccgtcag atggcgaatg atgtaatgaa
     1561 tgtcacggct tttattctgc caacgcagac gcagcggcag gtcgctgata acgcgcatca
     1621 gcagagattc ccagccgtga tcggggtgaa agtctttcgc cagcgggcgc ggaatagtac
     1681 gaaaacggcg ctcaggctgg gaggtaaaga taaaaagccg ctcgggagta agcgagcggt
     1741 ggtcaaataa ccgacagtac acggagttga aaaaactctc cgcaatctcg aagcgcgggt
     1801 aatccggcaa cagccgggtg taatgctctt tgacacgcag taaaaatgcc gcgtccgtgc
     1861 tttggccgtt ggtaatgcag cgcagttgct ccaccaccag acccacgtga tgatcataaa
     1921 gatggatacg gtttttcatc gcctgctgga cggcgtgcca gtcggcttgt tcgaaacgct
     1981 gctgcgcccc ggaagtcact tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa
     2041 tggtttgagc aatcagtaat tccaggccac gcggcattta gcgccctcat caggagcaga
     2101 gaattgccgg atgcggcttg aacgccttat ccagcctaca gtcggcaacg attgtaggct
     2161 ggataagacg cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat gcggcgtaaa
     2221 tgccttatcc ggcctacagt cagcaacgtt tgtaggttgc ttagaactgc gattcttcag
     2281 tggagccggt cagcgcggtg actgaagacg tgccgccctg aataatagtc gtcactttat
     2341 cgaagtaacc tgtacccact tcctgctggt gagatacgaa ggtatagcca tctttcgcgg
     2401 cggcaaattc cggctgctgc actttctcaa cgtagtgctt cataccctcg ccctgggcat
     2461 aggcgtttgc caggtcaaac atgttgaacc acatgctgtg gatacctgcc agggtgatga
     2521 actggaactt gtagcccata tccgacagct gctgctggaa gctggcaata gttttgtcgt
     2581 cgaggttttt ctgccagttg aacgacggcg agcagttata agccagcagt ttgcccggat
     2641 atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc cagatccggc gtggaggttt
     2701 cacaccagac caggtcagca tatggcgcat acgccaggcc acggctgatc gcttgctcaa
     2761 tgcccgcatg agtacggaag aagccttcac tggtacgctc gccagtaata aattcactgt
     2821 catacgggtc gcaatcggag gtaatcagat ccgccgcatc ggcatcggta cgagcaacca
     2881 gcagggttgg aacgcccgtc acgtcagctg ccagacgcgc cgcgaccagt ttctgaatag
     2941 cttcctgagt tggcactaaa actttgccgc ccatgtgacc gcatttcttc actgacgcca
     3001 gctgatcttc gaagtgaact gccgctgcac cggcttcaat catcgctttc atcagttcaa
     3061 aggcattcag gacaccgcca aaaccggctt ccgcatcggc aacgatcggc aggaagtaat
     3121 cgacatagcg cggatcgccc ggctcaatgc ccgcggacca ttggatctga tccgcacggc
     3181 ggaaagtatt gttgatccgc tccaccacag ctggcaccga gtttgccgga tagagcgact
     3241 gatccggata catgctggcc gccaggttag cgtccgccgc tacctgccat cccgacagat
     3301 agactgcttc aatacccgct ttcgcctgtt gcagcgcctg accgccagtc agtgcaccga
     3361 ggctgttgat gtagcctttt ttcgactcgc cgtgcagcag acgccacatt ttcgccgcgc
     3421 ccagttgcgc cagggtgcat tcaggattga ctgaaccgcg taatttcacc acatcttccg
     3481 cgctgtatgg gcgagtaatg ccttcccaac gcggttgagt ccactctttc tgtaattctt
     3541 caatttgttg tgtacgggtt ttcatgtgca gatgctccat agttatgtgg tggtttacgc
     3601 taacaggcgg tagcctggca gggtcaggaa atctattaac tcatcggaag tggtgatctg
     3661 ttccatcaag cgtgcggcat cgtcaaaacg cccctgggag aaacgttctt cgcccaattc
     3721 gctagcaatg actttcatct cttcgtccag catctggcgg aacaaggctt tggtcaccgg
     3781 tttgccattg ctcaacgttt tttgatgatg gatccactgc cagatcgagg tacgggaaat
     3841 ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc acgcagccgt tgccggagat
     3901 ccacgcttcg atgtactgca cagccacgcg aatgttggcg cgcatacctt cttcggtgcg
     3961 ttcaccatca caaggtgcca gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcaa
     4021 tacttcaagc tgatttttac gggagccgag aatgtcgttg aataccgcca ttgccgtatc
     4081 cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg ttattggctt ccagcgattt
     4141 atccgctttt actttgttga gcacctggtt attgtgctct tcatctttgc tcggaataaa
     4201 cgccgccatg ccgcccatcg caaaagcacc gcgtttatgg caggttttaa tcaacaggcg
     4261 tgagtaagca ttcaggaagg gtttatccat cgtcactgcc tgtctgtctg gcaggacgcg
     4321 atcgggatag tttttcaacg ttttgatata gctgaagatg taatcccaac gaccgcagtt
     4381 cagaccaaca atatggtcac gcagcgcgtg aaggatttca tccatctgga acacggcggg
     4441 cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc
     4501 tgcatagctg aagacttcgc tccaccaggc cgcttcctgc caggactggg ttttcggcag
     4561 atagaaatag ggaccgctgc cctttgccaa cagtgcctga tagttgtgga agaaatagag
     4621 cgcaaaatca aacaggctac cggggattgc ctcaccacgc caggtgacat gtttttccgg
     4681 caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga ttgggcttga gctgataaat
     4741 tttgcctgct tcattggtat agctgatggt gccgttaact gcatcacgca ggttaatttg
     4801 cccgtcgatc actttgttcc agtccggggc cagtgaatct tcgaaatcgg ccataaagac
     4861 tttcacattg gcgttgagcg cgttgatcac catcttgcgc tctaccgggc cagttatctc
     4921 tactcgacga tcttctaagt ccgtaggaat cccgcgaatt ttccagtcag catcacgaat
     4981 ggaagctgtt tccgaaataa aatcaggcaa cgttccgtta tcaatatctt gctgctgctg
     5041 aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa tgcgtcacca gctcaatcag
     5101 aaactctacc gcttcggcag taagaatttg cttctcctgc tcgccatacg gccttgtgaa
     5161 agccagttca tcggtttttg ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc
     5221 gaaaacttcc cctctgtgaa cgaaggatcg ttgtgcgctt ttcgttcagc acaactaa
//
LOCUS       DAJSKJ010000210         5185 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000210 DAJSKJ010000000
VERSION     DAJSKJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.214"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..908
                     /gene="alsB"
                     /locus_tag="ON345_003534"
     CDS             <1..908
                     /gene="alsB"
                     /locus_tag="ON345_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HCR3073535.1"
                     /translation="TLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKKGIEDEAKTLG
                     VSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVARAWKKGIYLVN
                     LDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVAIIEGKAGNAS
                     GEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIKAIYCANDTMA
                     MGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIGATGLKLMVDA
                     EKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            1035..2567
                     /gene="alsA"
                     /locus_tag="ON345_003535"
     CDS             1035..2567
                     /gene="alsA"
                     /locus_tag="ON345_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HCR3073536.1"
                     /translation="MATTYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSINQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            2546..3526
                     /gene="alsC"
                     /locus_tag="ON345_003536"
     CDS             2546..3526
                     /gene="alsC"
                     /locus_tag="ON345_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HCR3073537.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            3537..4232
                     /gene="alsE"
                     /locus_tag="ON345_003537"
     CDS             3537..4232
                     /gene="alsE"
                     /locus_tag="ON345_003537"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HCR3073538.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            4216..5145
                     /gene="alsK"
                     /locus_tag="ON345_003538"
     CDS             4216..5145
                     /gene="alsK"
                     /locus_tag="ON345_003538"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HCR3073539.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
BASE COUNT         1321 a         1243 c         1353 g         1268 t
ORIGIN      
        1 gcacactcgt gggcttaatg ttgtcaacca gcgcttttgc tgccgccgaa tatgctgtcg
       61 tattgaaaac cctctccaac ccattttggg tagatatgaa aaaaggcatt gaagatgaag
      121 caaaaacgct gggcgtcagc gttgatattt ttgcctctcc ttcagaaggc gattttcaat
      181 ctcaattgca gttatttgaa gatctcagta ataaaaatta caaaggtatc gccttcgctc
      241 cattatcctc agtgaatctg gtcatgcctg tcgcccgcgc atggaaaaaa ggcatttatc
      301 tggttaatct cgatgaaaaa atcgacatgg ataatctgaa aaaagctggc ggcaatgtgg
      361 aaggttttgt caccaccgat aacgttgctg tcggggcgaa aggcgcgtcg ttcattattg
      421 acaaattggg tgctgaaggt ggtgaagtcg caatcattga gggtaaagcc ggtaacgcct
      481 ccggtgaagc gcgtcgtaat ggtgccaccg aagccttcaa aaaagcaagc cagatcaagc
      541 ttgtcgccag ccagcctgcc gactgggacc gcattaaagc actggatgtc gccactaacg
      601 tgttgcaacg taatccgaat attaaagcga tctattgcgc gaatgacacg atggcaatgg
      661 gtgttgctca ggcagtcgca aacgccggaa aaacgggcaa aatattggtc gtcggtacag
      721 atggaattcc ggaagcccgc aaaatggtgg aagccggaca aatgaccgcg acggttgccc
      781 agaacccggc agatattggc gcaacgggtc tgaagctgat ggttgacgct gagaaatccg
      841 gcaaggttat tccgctggat aaagcaccgg aatttaaact ggtcgattca atcctggtca
      901 ctcaataaaa ctgaatgatg ccggatgatg atgctaatga agtgtcttat ccggccaaca
      961 aaataacctg aacgtagacc tgataaacgt agccatcagg caataatacg atatttcgtg
     1021 cagagaggtt atttatggcc acgacatata tatcgatggc ggggatcggc aagtcctttg
     1081 gtccggttca cgcattaaag tcggttaatt taacggttta tcctggtgaa atacatgcat
     1141 tactaggaga aaatggcgcg ggtaaatcca cgctaatgaa ggttttatcc ggaatacatg
     1201 agccgaccaa aggcaccatt actattaata acattaacta taacaagctg gatcataaat
     1261 tagcggcaca actcggtatc gggattattt atcaggaact cagcgttatt gatgaattaa
     1321 ccgtactgga aaatttatat attggtcgtc atctgacgaa aaaaatctgt ggcgtcaata
     1381 ttatcgactg gcgagaaatg cgtgtccgcg ccgccatgat gttattacgc gtgggcttga
     1441 aagttgattt agatgagaaa gtggcgaatt tatctatcag ccacaagcag atgctggaaa
     1501 ttgccaaaac gctgatgctc gatgccaaag tcatcatcat ggatgaaccc acctcctcac
     1561 tcaccaataa agaggtggac tatctgtttc tgatcatgaa tcagttgcgt aaagagggta
     1621 cggccatcgt ctatatctcg cataagttgg cggaaattcg ccgtatttgc gaccgctata
     1681 cggtgatgaa agacggcagc agcgtttgca gcggcatggt gagcgatgtg tcaaatgacg
     1741 atatcgtccg tctgatggta ggccgcgaat tgcaaaaccg ttttaacgcg atgaaggaga
     1801 atgtcagcaa ccttgcgcac gaaacggttt ttgaggtgcg caacgtcacc agccgtgaca
     1861 gaaaaaaggt ccgggatatc tcatttagcg tctgccgggg agaaatatta ggctttgccg
     1921 ggctggtcgg ttccggacgt actgaactga tgaattgcct gtttggcgtt gataaacgcg
     1981 ctggcggaga aatccgtctt aatggcaaag atatctctcc acgttccccc ctggatgccg
     2041 tgaaaaaagg gatggcttac atcacagaaa gccgccggga taacggtttt ttccccaact
     2101 tttccatcgc tcagaacatg gcgatcagcc gcagtctgaa agacggcggc tataaaggcg
     2161 cgatgggctt gtttcatgaa gttgacgagc aacgtaccgc tgaaaatcaa cgcgaactgc
     2221 tggcgctgaa atgtcattcg ataaaccaga atatcaccga actctccggg ggaaatcagc
     2281 agaaagtcct gatctccaaa tggctgtgct gttgcccgga agtgattatt ttcgatgaac
     2341 ctacccgcgg catcgacgtt ggcgcgaaag ccgaaattta caaagtgatg cgccaactgg
     2401 cggacgacgg aaaagtcatc ctgatggtgt catctgaact acctgaaatt atcaccgtct
     2461 gcgaccgcat cgccgtgttc tgcgaaggac gactgacgca aatcctgacg aatcgcgatg
     2521 acatgagcga agaggagatt atggcatggg ctttaccaca agagtaaaaa gcgaagcgag
     2581 cgagaagaaa ccgttcaact ttgcgttgtt ctgggataaa tacggcacct tttttatcct
     2641 ggcgatcatc gtcgccatct ttggttcgct gtcaccagaa tattttctga ccaccaataa
     2701 tattacccag atttttgttc aaagctccgt gacggtattg atcggcatgg gcgagttttt
     2761 cgctatcctg gtcgctggta tcgacctctc ggttggcgca attctggcgc tttccggtat
     2821 ggtgaccgcc aaactgatgt tggcaggtgt tgacccattt ctcgcagcga tgattggcgg
     2881 tgtactggtt ggcggagcac tgggggcgat caacggctgc ctggtcaact ggacggggct
     2941 acacccgttc atcatcaccc ttggcaccaa cgcgattttc cgtgggatca cgctggtgat
     3001 ctccgatgcc aactcggtat acggcttctc atttgacttc gtgaacttct ttgccgccag
     3061 cgtaattggg atacctgtcc ccgttatctt ctcgctaatt gtcgcgctca tcctttggtt
     3121 tctgacaacg cgtatgcggc tcgggcgcaa catctacgca ctgggcggca acaaaaattc
     3181 ggcgttctat tccgggattg acgtgaaatt ccacatcctg gtggtgttta tcatctccgg
     3241 tgtttgtgca ggtctggcag gcgtcgtctc aactgcacga ctcggtgccg cagaaccgct
     3301 tgccggtatg ggttttgaaa cctatgccat tgccagcgcc atcattggcg gcaccagttt
     3361 cttcggcggc aaggggcgca ttttctctgt ggtgattggc gggttgatca tcggcaccat
     3421 caacaacggt ctgaatattt tgcaggtaca aacctattac caactggtgg tgatgggcgg
     3481 attaattatc gcggctgtcg cccttgaccg tcttatcagt aagtaaggaa ttgaacatga
     3541 aaatctcccc ctcgttaatg tgtatggatc tgctgaaatt taaagaacag atcgaattta
     3601 tcgacagcca tgccgattac tttcacatcg atatcatgga tggtcacttt gtccccaatc
     3661 tgacactctc accgttcttc gtaagtcagg ttaaaaaact ggcaactaaa ccgctcgact
     3721 gtcatctgat ggtgacgcgg ccgcaggatt acattgctca actggcgcgt gcgggagctg
     3781 atttcatcac tttgcatccg gaaactatca acggtcaggc gttccgcctg attgatgaaa
     3841 tccgctgtca tgacatgaaa gtggggctga tccttaaccc ggagacacca gttgaggcca
     3901 tgaaatacta tatccataag gccgataaaa ttacggtcat gactgtcgat cccggctttg
     3961 ccggacaacc gttcattcct gaaatgctgg ataaacttgc cgaactgaag gcatggcgtg
     4021 aacgagaagg tctggagtac gaaattgagg tggacggttc ctgcaaccag gcaacttacg
     4081 aaaaactgat ggcggcaggg gcggatgtct ttatcgtcgg cacttccggc ctgtttaatc
     4141 atgcggaaaa tatcgacgaa gcatggagaa ttatgaccac gcagattctg gctgcaaaaa
     4201 gcgaggtaca gcctcatgca aaaacagcat aacgtcgtag cgggcgtgga tatgggggca
     4261 acgcatatcc gcttttgtct gcggacagca gaaggtgaaa cgctacactg cgaaaaaaag
     4321 cggaccgcag aagtcattgc tcccggcctg gtgtcgggta tcggcgaaat gattgacgag
     4381 caactcaggc gctttaacgc tcgctgtcat ggtctggtga tgggatttcc ggcgctggtc
     4441 agtaaagata aacgcaccat aatttctacg cctaacctgc cgttaacagc ggcggattta
     4501 tatgatctcg ccgataagct cgaaaatacg ctgaattgtc cggttgagtt ttcccgcgac
     4561 gttaacctgc aactctcctg ggacgtagta gaaaaccgcc ttacgcaaca actggttctg
     4621 gcggcctatc tcggtacggg gatggggttc gcagtgtgga tgaacggtgc gccgtggacg
     4681 ggtgcacacg gtgtggcagg cgaactgggt catatccccc tgggagatat gacccaacac
     4741 tgcgcgtgtg gcaatcctgg gtgcctggaa accaattgct ctggaatggc gctaagacgc
     4801 tggtacgaac aacagccccg aaattaccca ttgagcgacc ttttcgtcca tgcggaaaac
     4861 gcccctttcg tccagagtct gcttgaaaac gcggcacggg ccattgccac cagcattaat
     4921 ctgttcgatc ccgatgcggt gattctgggc ggtggcgtga tggatatgcc cgccttccca
     4981 cgcgagactc tcattgccat gacccaaaag tacctgcgcc gtccactgcc gcatcaggtc
     5041 gtgcgcttta ttgccgcctc atcttctgac tttaatggcg ctcagggtgc agcaatattg
     5101 gcgcatcaac attttttgcc acagtcctgt gctaaagccc catgagtaaa gcgttggcac
     5161 agtagcgcaa tgtcccgctg tgcca
//
LOCUS       DAJSKJ010000211         5137 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000211 DAJSKJ010000000
VERSION     DAJSKJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.215"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            246..1190
                     /gene="leuO"
                     /locus_tag="ON345_003539"
     CDS             246..1190
                     /gene="leuO"
                     /locus_tag="ON345_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR3073540.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1344..1388)
                     /locus_tag="ON345_003540"
     CDS             complement(1344..1388)
                     /locus_tag="ON345_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073541.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1507..3231
                     /gene="ilvI"
                     /locus_tag="ON345_003541"
     CDS             1507..3231
                     /gene="ilvI"
                     /locus_tag="ON345_003541"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR3073542.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3234..3725
                     /gene="ilvN"
                     /locus_tag="ON345_003542"
     CDS             3234..3725
                     /gene="ilvN"
                     /locus_tag="ON345_003542"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR3073543.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3737..3823
                     /locus_tag="ON345_003543"
     CDS             3737..3823
                     /locus_tag="ON345_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR3073544.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3905..4909
                     /gene="cra"
                     /locus_tag="ON345_003544"
     CDS             3905..4909
                     /gene="cra"
                     /locus_tag="ON345_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR3073545.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWRDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1263 a         1160 c         1338 g         1376 t
ORIGIN      
        1 gtattcgcgg tagttatgat tagattgttt tcgcaacaaa aacattatgg attattatgc
       61 tgtggtaaat gactcattcc acggcaatgg attctgtttt tatcagaacc cgtatcttta
      121 tgttttccga attttactca ttttgctttt tcttatttta tatgcatgat aaatcatatt
      181 cttcaggatt atttctctgc attccaataa gggaaaggga gttaagtgtg acagtggagt
      241 taagtatgcc agaggtacaa acagatcatt cagagacggc ggagttaagc aagccacagc
      301 tacgcatggt cgatctcaac ttattaaccg ttttcgatgc cgtgatgcag gagcaaaaca
      361 ttacccgtgc cgctcatgtt ctgggtatgt cgcaacctgc ggtcagtaac gctgttgcac
      421 gcctgaaggt gatgtttaat gacgagcttt ttgttcgtta tggccgtggt attcaaccga
      481 ctgctcgcgc atttcaactt tttggctcag ttcgtcaggc attgcaacta gtacaaaatg
      541 aattgcctgg ttcaggtttt gaacccgcga gcagtgaacg tgtatttcat ctttgtgttt
      601 gcagcccgtt agacagcatt ctgacctcgc agatttataa tcacattgag cagattgcgc
      661 caaatataca tgttatgttc aagtcttcat taaatcagaa cactgaacat cagctgcgtt
      721 atcaggaaac ggagtttgtg attagttatg aagacttcca tcgtcctgaa tttaccagcg
      781 tgccattatt taaagatgaa atggtgctgg tagccagcaa aaatcatcca acaattaagg
      841 gcccgttact gaaacatgat gtttataacg aacaacatgc ggcggtttcg ctcgatcgtt
      901 tcgcgtcatt tagtcaacct tggtatgaca cggtagataa gcaagccagt atcgcgtatc
      961 agggcatggc aatgatgagc gtacttagcg tggtgtcgca aacgcatttg gtcgctattg
     1021 cgccgcgttg gctggctgaa gagttcgctg aatccttaga attacaggta ttaccgctgc
     1081 cgttaaaaca aaatagcaga acctgttatc tctcctggca tgaagctgcc gggcgcgata
     1141 aaggccatca gtggatggaa gagcaattag tctcaatttg caaacgctaa ctgaatgcag
     1201 aataggtcag acatgaatgt ctggtttatt ctgtattttt tattgaatgt agaattttat
     1261 tctgaatgtg tgggctctct attttaggat taattaaaaa atagagaaat tgctgtaagt
     1321 tgcgggattc agccgattta ttatcaattt aatccttcgt aatggaggat tttatcgttt
     1381 cttttcacct ttcctcctgt ttattcttat taccctgcgt ttatgtctct ggctgccaat
     1441 tgcttaagca agatcggacg gttaatgtgt tttacacatt ttttccgtca aacagtgagg
     1501 caggccatgg agatgttgtc tggagccgag atggtcgtcc gatcgcttat cgatcagggc
     1561 gttaaacaag tattcggtta tcccggaggc gcagtccttg atatttacga tgcattgcat
     1621 accgtgggtg gtatcgatca tgttttagtt cgtcatgagc aggcggcagt gcatatggcc
     1681 gatggtctgg cgcgcgcgac cggggaagtc ggcgtcgtgc tggtaacgtc gggtccaggg
     1741 gcgaccaatg cgattaccgg catcgccacc gcttatatgg attccattcc attagttgtc
     1801 ctttccgggc aggtagcgac ctcgttgata ggttacgatg cctttcagga gtgcgacatg
     1861 gtggggattt cgcgaccggt ggttaaacac agttttctgg ttaagcaaac ggaagacatt
     1921 ccgcaggtgc tgaaaaaggc tttctggctg gcggcaagtg gtcgcccagg accagtggtc
     1981 gttgatttac cgaaagatat tcttaatccg gcgaacaaat taccctatgt ctggcctgag
     2041 tcggtcagta tgcgttctta caatcccact actaccggac ataaagggca aattaagcgc
     2101 gctctgcaaa cgctggtggc ggcaaaaaaa ccggttgtct acgtaggcgg tggggcaatc
     2161 acggcgggct gccatcaaca gttgaaagaa acggtggagg cgttgaatct gcccgttgtt
     2221 tcctcattga tggggctggg ggcgtttccg gcaacgcatc gtcaggcact gggcatgctg
     2281 ggaatgcacg gtacctacga agccaatatg acgatgcata acgcggatgt gattttcgcc
     2341 gtcggggtac gatttgatga ccgaacgacg aacaatctgg caaagtactg cccaaatgcc
     2401 actgttctgc atatcgatat tgatcctact tccatttcta aaaccgtgac tgcggatatc
     2461 ccgattgtgg gggatgctcg ccaggtcctc gaacaaatgc ttgaactctt gtcgcaagaa
     2521 tccgcccatc aaccactgga tgagatccgc gactggtggc agcaaattga acagtggcgc
     2581 gctcgtcagt gcctgaaata tgacactcac agtgaaaaga ttaaaccgca ggcggtgatc
     2641 gagactcttt ggcggttgac gaagggagac gcttacgtga cgtccgatgt cgggcagcac
     2701 cagatgtttg ctgcacttta ttatccattc gacaaaccgc gtcgctggat caattccggt
     2761 ggcctcggca cgatgggttt tggtttacct gcggcactgg gcgtcaaaat ggcgttgcca
     2821 gaagaaaccg tggtttgcgt cactggcgac ggcagtattc agatgaacat tcaggaactg
     2881 tctaccgcgt tgcaatacga gttgcccgta ctggtggtga atctcaataa ccgctatctg
     2941 gggatggtga aacagtggca ggacatgatc tattccggcc gtcattcaca atcttatatg
     3001 caatcactac ccgatttcgt ccgtctggcg gaagcctatg gtcacgtcgg gatccagatc
     3061 tctcatccgc atgagctgga aagcaaactt agcgaggcgc tggaacaggt gcgcaataat
     3121 cgcctggtgt ttgttgatgt taccgtcgat ggcagtgagc acgtctaccc gatgcagatt
     3181 cgcgggggcg gaatggatga aatgtggtta agcaaaacgg agagaacctg attatgcgcc
     3241 ggatattatc agtcttactc gaaaatgaat caggcgcgtt atcccgcgtg attggccttt
     3301 tttcccagcg tggctacaac attgaaagcc tgaccgttgc gccaaccgac gatccgacat
     3361 tatcgcgtat gaccatccag accgtgggcg atgaaaaagt gcttgagcag atcgaaaagc
     3421 aattacacaa gctggtcgat gtcttgcgcg tgagcgagtt ggggcagggc gcgcatgttg
     3481 agcgggaaat catgctggtg aaaattcagg ccagcggtta cgggcgtgac gaagtgaaac
     3541 gtaatacgga aatattccgt gggcaaatta tcgatgtcac accctcgctt tataccgttc
     3601 aattagcagg cactagcgat aagcttgatg catttttagc atcgattcgc gaggtggcga
     3661 aaattgtgga agttgctcgt tctggtgtgg tcggactttc gcgcggcgat aaaataatgc
     3721 gttgagaatg atctgaatgc gcaatttgca gcccaacatg tcacgttggg ctttttttgc
     3781 gaaatcagtg ggaacctgga ataaaagcag ttgccgcagt taattttatg cgcttagatg
     3841 ttaatgaatt taacccatac cagtacaatg gctatggttt ttacatttta cgcaaggggc
     3901 aattgtgaaa ctggatgaaa tcgctcggct ggcgggagtg tcgcggacca ctgcaagcta
     3961 tgttattaac ggcaaagcga agcaataccg tgtgagcgac aaaaccgttg aaaaagtcat
     4021 ggctgtggtg cgtgagcaca attaccaccc gaacgccgtg gcagctgggc ttcgtgctgg
     4081 acgcacacgt tctattggtc ttgtgatccc cgatctggag aacaccagct atacccgcat
     4141 cgctaactat cttgaacgcc aggcgcggca acggggttat caactgctga ttgcctgctc
     4201 agaagatcag ccagacaacg aaatgcgctg tattgagcac cttttacagc gtcaggtcga
     4261 tgccattatt gtttcgacat cgttgcctcc tgagcatcct ttttatcaac gctgggctaa
     4321 cgacccgttc ccgattgtcg cgctggaccg cgccctcgat cgtgaacact tcaccagcgt
     4381 ggttggtgcc gatcaggatg atgccgaaat gctggcggaa gagttacgta agtttcctgc
     4441 cgagacggtg ctttatcttg gcgcgttgcc ggagctttct gtcagcttcc tgcgtgaaca
     4501 aggtttccgt actgcctgga gagatgatcc gcgcgaagtg catttccttt atgccaacag
     4561 ctatgagcgg gaggcggctg cccagttatt cgaaaaatgg ctggaaacgc atccgatgcc
     4621 gcaggcgctg ttcacaacgt cgtttgcgtt gttgcaaggg gtgatggatg tcacgctacg
     4681 tcgcgacggc aaactacctt ctgacctggc aattgccacc tttggcgata acgaactgct
     4741 cgacttctta cagtgcccgg tgctggcagt ggctcaacgt caccgagatg tcgcagagcg
     4801 tgtgctggag attgtcctgg caagcctgga cgaaccgcgt aagccaaaac ctggtttaac
     4861 gcgcattaag cgtaatctct atcgccgcgg cgtgctcagc cgtagctaag ccgcaaacaa
     4921 aaatacgcgc caggtgaatt tccctctggc gcgtagagta cgggaatgga catcaatatc
     4981 cttaaagtaa ataagactat tcctgactat tattgataag ttcttttaaa cccgcccgtt
     5041 aattaactca ccagctgaaa ttcacaataa ttaagtgata tcgacagcgc gtttttgcat
     5101 tattttgtta catgcagaga tgaattgccg atttaac
//
LOCUS       DAJSKJ010000212         5070 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000212 DAJSKJ010000000
VERSION     DAJSKJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.216"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            78..1994
                     /gene="mngA"
                     /locus_tag="ON345_003545"
     CDS             78..1994
                     /gene="mngA"
                     /locus_tag="ON345_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR3073546.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGSQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLGDKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            2012..4645
                     /gene="mngB"
                     /locus_tag="ON345_003546"
     CDS             2012..4645
                     /gene="mngB"
                     /locus_tag="ON345_003546"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR3073547.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSECSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1247 a         1168 c         1377 g         1278 t
ORIGIN      
        1 gtgaaattaa tcacataatg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
       61 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgtgtctga
      121 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
      181 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
      241 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
      301 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gtggatggcc
      361 cggaagcggt tgatttagtg gtgttgctgg cgattccccc caatgaagcg ggtactacgc
      421 acatgcaact gctgacagcg ctgaccacgc gcctggctga tgatgagatc agggcgcgta
      481 tacagtcggc gacgacgcct gatgaattgc tctcggcgct ggatgacaag ggaggctcgc
      541 aaccttctgc ctctttttcc aacgcgccaa ctatcgtttg cgtaacggcc tgtccggcgg
      601 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
      661 taaatgttta cgttgaaaaa caaggtgcta acggcattga agggcgttta acggcggatc
      721 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
      781 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
      841 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
      901 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
      961 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggttgca gtattactgt
     1021 cgcaaatctt cgggctacaa gatttgttta atgaagaaaa ctcctggctg tggatgtacc
     1081 gcaaactggg cggcgggatg ctcgggattt tgatggtgcc ggtgctcgcg gcctataccg
     1141 cctattcact gggtgataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
     1201 acatgatcgg ttccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
     1261 tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggcttc ctgacttttt
     1321 atctctaccc ggtgctcggt acattgggag cgggcagtct gatgctgttt gtggtggggg
     1381 aacctgtcgc ctggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
     1441 acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc ctttgacctt ggagggccag
     1501 tgaataaagc cgcttatgca ttctgcctgg gcgcaatggc gaacggcgtt tacggcccgt
     1561 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
     1621 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
     1681 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
     1741 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
     1801 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
     1861 gcggtgttat ggcggcaatt ggctggtttg gcgcggcatt ggtgggggct gcaatctcga
     1921 ctgcaattct cctgatgtgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
     1981 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagca gtatctcgcg ttcacatcac
     2041 cccgcatatg cactgggatc gagagtggta tttcaccacc gaagagtcac gtattctgct
     2101 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
     2161 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
     2221 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
     2281 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
     2341 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt cctttggcat
     2401 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
     2461 cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
     2521 tagcgaagtg acggcgcagg tgctgccgct gggctacgcc atcggtaagt acttacctgc
     2581 cgacgaaaac ggattacgta aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
     2641 tgtaaccaaa gagattttgc tgccgaatgg gcatgaccag atgccattgc agcaaaatat
     2701 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
     2761 ctttgaagag gtatttgaga agatcgaagc gcagcgtgaa agtctggcca ccctgaaagg
     2821 tgaatttatc gatggcaaat atatgcgcgt acatcgcacc atcggttcca cgcgcatgga
     2881 tatcaaaatt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
     2941 aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
     3001 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
     3061 tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
     3121 ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac tcgtgctgtt
     3181 taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcggc tgcgcgccag
     3241 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
     3301 tgagatcgat cctggcctaa tcgatcggca aattgttcat tatggcaatt atgatccctt
     3361 tatggagttt gatattcaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
     3421 tattgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggattct
     3481 ggaaaatgct ttctggcaga ttgcgctcaa tgaggatggt tctctgcaac tggtagataa
     3541 agacagcggt gtgcgctatg accgggtatt gcaaattgaa gaaagctctg atgatggtga
     3601 tgaatatgac tattcacccg caaaagaaga gtgggtaatt accgcagcga acgcgaaacc
     3661 gcaatgcgat attattcatg aagcctggca gagcagggct gttatccgct atgacatggc
     3721 agtgccgctc aatttgtcag aatgcagcgc ccggcaatcc actggcagag taggggtggt
     3781 gttggttgtc actcttagtc ataacagcag gcgtattgat gtggatatca accttgataa
     3841 ccaggctgac gatcatcgcc ttcgtgtcct ggtccctaca ccttttaaca ccgacagtgt
     3901 tctggcagat acgcagtttg gttcgctaac gcgccccgtg aacgacagtg caatgaacaa
     3961 ctggcagcaa gaaggctgga aagaagcgcc ggttccggta tggaatatgc tcaactatgt
     4021 tgccttacag gaagggcgta acggcatggc tgtctttagc gaagggttac gtgaatttga
     4081 agtcatcggt gaagagaaga aaacctttgc cattacgttg ctgcgtggcg tgggcttact
     4141 ggggaaagaa gatctgcttt taaggcctgg gcggccttcg ggaattaaaa tgccagtccc
     4201 ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt ttattgagtt ataccggtac
     4261 gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg ctgactccag tacagtgtta
     4321 caacaaaatc ccatgggatg cgatgaagct caacaaagcc ggattcaacg tgccggaaag
     4381 ttatagtttg ttgaaaatgc ccccagtggg atgcctgata agcgcactta agaaagctga
     4441 agaccgacaa gaagtgattt tacggctgtt taatccggct gaatcagcaa cctgtgatgc
     4501 gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa acgatgatgg atgaacacat
     4561 taccaccgag gaaaatcaag gttcaaatct atcggggcct tttttacccg gccagtcacg
     4621 gacgttcagt taccggcttg cctgaatagc aatcaaaccg aagccacata tgcgcggcca
     4681 gattgttgac aaagggcgct ttgttcatgc cggatacggc atgaacgctt tattcggtct
     4741 acaaaagcag gcaaattcaa tatattgcag agatgatgta gacactggca agcgtagcgc
     4801 atcaggtaat tttgcgttta tcttcactct caagccacgt atatgtggct ttatttttaa
     4861 caaaataata accctgggtg agttaattat aatataatta taagttaatt aaatgttaat
     4921 attggcgggg tggatttatg cctttattag taatcctgaa actctgcgtc gtattagcca
     4981 gtgaccaaaa aaagaattaa ggtcaaccgt gctgtttttg cttcgtctct ttttatcttt
     5041 aattgccaac cgaaactaat ttcagcctta
//
LOCUS       DAJSKJ010000213         5045 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000213 DAJSKJ010000000
VERSION     DAJSKJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.217"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(594..670)
                     /locus_tag="ON345_003547"
     tRNA            complement(594..670)
                     /locus_tag="ON345_003547"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Arg,seq:tct)
     gene            complement(684..764)
                     /locus_tag="ON345_003548"
     tRNA            complement(684..764)
                     /locus_tag="ON345_003548"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(772..847)
                     /locus_tag="ON345_003549"
     tRNA            complement(772..847)
                     /locus_tag="ON345_003549"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(812..814),aa:Ile,seq:cat)
     gene            complement(888..1946)
                     /locus_tag="ON345_003550"
     CDS             complement(888..1946)
                     /locus_tag="ON345_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR3073548.1"
                     /translation="MLNTVKISSYELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGCEAKDRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEWPHHQLVSTYSELNRQYVSLQEAYKTLRRYFSVSAAVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLRQIISASSRPGDLIADFFMGSGSTVKAALSLGRRAIGVELET
                     GRFEQTVREVQDFISQNG"
     gene            complement(2097..2294)
                     /locus_tag="ON345_003551"
     CDS             complement(2097..2294)
                     /locus_tag="ON345_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCR3073549.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(2507..3196)
                     /locus_tag="ON345_003552"
     CDS             complement(2507..3196)
                     /locus_tag="ON345_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR3073550.1"
                     /translation="MNNQYLQFVREQLMIATADLTGSTKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPAEFSTSSWRRAVLSLEEHHK
                     AWLLWCYNGNICWEHQIAITQWVWNEFKTRYGTRKIAGKTQERVKKLIWLAAQDVRGW
                     VIGREVYQRLELARLCGIKPDNWSHNYANYWREMCDIFKNLDTESLICTVKMRSQQKA
                     TFSRRDIAKVN"
     gene            complement(3193..3552)
                     /locus_tag="ON345_003553"
     CDS             complement(3193..3552)
                     /locus_tag="ON345_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR3073551.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLNLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVRGR
                     PVSGGRLEIKITEVGSA"
     gene            complement(3565..4614)
                     /locus_tag="ON345_003554"
     CDS             complement(3565..4614)
                     /locus_tag="ON345_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR3073552.1"
                     /translation="MRVLLRPVLVPELGLVVLTPGRESLPVFHCGRVLVEPEPKNMRA
                     LPSGAVPTVRQPLVEDKSLLPFFSDERVNRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLREQTSDSLEQLAQQNLAAWMIDVIRHAMNGTQERE
                     LSLAELCWWAVCHQVADAIPESVLRRSLGLPVEKIRSVYSESDIVPGEQTATSILKQR
                     TKNIALPPHVHQQQNPPQKKAVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADEPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            complement(4616..4888)
                     /locus_tag="ON345_003555"
     CDS             complement(4616..4888)
                     /locus_tag="ON345_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073553.1"
                     /translation="MAQHIKSHNSEAAPTTKQGRRFRVPQYGWFHYLFCSTDEADMLQ
                     QAYWRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
BASE COUNT         1212 a         1412 c         1179 g         1242 t
ORIGIN      
        1 cgctttagct catctgcgct cctggctgaa accacatcgt aatatttaaa tgccatgaat
       61 cctcccggcc gggatagtat gataaaaact gactgaagtc tgaaaacaca aaagacaact
      121 gccagtaaaa aagaaaaaag ccgcgcagtt gcgcagcctc atcaccatcg ggtattattc
      181 acacggtgaa taatcgaaac tcactttagt ttgtttttac tccttgagcc cgccccctac
      241 gcgggctttt tttgcatgta aaaagccccc tccgaagagg gggctggtga tatgcctaat
      301 ctctgtatac tgcatggtgc cgggtgcctc ccggtagatt cagcacggta actgaacctg
      361 cgaattgtga ccattggcat tgagaatatg ctttaccgtt cgccccgccg ctgaggggga
      421 ttcaccatgc gacacgattt ttaacaaatt caccaccagc cagacaatca taaacttgct
      481 gaattgtgag gtatttaaaa atttccccga ttaactgata ccctgctaac cgtctggtgt
      541 tttctttttc agcaacttaa aaggcccgcc gaagcgagcc aggaaaataa gtgtggcgcg
      601 ttgtactgga gtcgaaccag tgaccaattg cttagaaggc aactgctctg tccggctgag
      661 ctaacaaagc agggtacaga taatggaccg ccatcgagga ctcgaacccc gcaccaacaa
      721 ccctgttatc gtgtcgtctg ctcttcctga tgagctaatg gcggtatgtg atggtggccc
      781 ttgctggatt tgaaccagcg acctggcgat tatgagtcac tcgctctcac cactgagcta
      841 aagggccggg cgcagaataa taacggtgca tcactgattc tgcaatatca tccgttctga
      901 ctgataaaat cctgaacttc cctgactgtc tgctcaaaac gtccggtctc cagctcaaca
      961 ccaatcgccc gacgtcccag tgacagcgcc gcttttaccg ttgaacctga ccccatgaaa
     1021 aaatctgcaa tcaggtcacc cggacgactg ctcgcactga ttatctgccg cagcatttct
     1081 gccggttttt cgcacggatg tttcccggga tagtactgta cgggtttgtg cgtccacaca
     1141 tccgtataag gaaccgcagc cgatacggaa aaataacgcc gcagggtttt atacgcttcc
     1201 tgcaggctga catattgtcg gttcagttca ctgtatgtac tgaccagctg gtggtgtggc
     1261 cattccagtt caccgcgctg atgcttctct tctgccaccc gggcaaacag ggcctgtaat
     1321 ttcagataat cgctttcgtt cggcagctgc cactgactgg cactgaacca gtgcgacacc
     1381 atgtttttct ttcctgtggc atctgcaatc tgttttgccg ttatccccag ggcagcgcgc
     1441 gcatcacgaa agtaagcaat cagcggggcc atcacatgct gtttcagtgc cctgtccttc
     1501 gcctcacagc catcatcttt cgggcgatac ggcccctgat aatgttccgc gaacagaatg
     1561 cgctctgtgg ccggaaaata cgcccgcagg ctttccttgt tacacccgtt ccagcgtccg
     1621 gacggctttg cccagatgat atggttcagt acgttaaacc gctcacgcat cattatttcg
     1681 atgtcagatg ccaggcgatg gccacagaac aggtaaagac ttccggctgg cttcagcacc
     1741 cgccagaact gcgcaagaca ctggtccagc cacttcaggt aatcttcatc acccgcccac
     1801 tggttatccc agccctcggg tttcactttg aagtacggcg ggtccgtgac tatcaggtct
     1861 acagaatttt cgggtaacga ccgcataaat tccaggcagt cggcgttgat taactcataa
     1921 ctggatattt ttacagtatt aagcatggat cattaagcct gtctctgata ggctcattct
     1981 gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa cccaacgcat gagcagatgg
     2041 ctggtgggtg ccccttacaa ccaccagccg cccatttacc acaaataaaa aagccttcag
     2101 gactgaaggc gtctgtaaca accgaactga tagtctgcca gacccgccat aaccagctgg
     2161 gtcagtatta actgacaacg ttcgcgtgaa aggtaagtat tctgcgcaat ttccccgaca
     2221 gtcgccggtt cggtgacgct caattcatta aacaccactc tggcagtttc ggtcatatcc
     2281 tgctgtttta gcatgtcttt ttcccttttc cggttaacgt gacacaccaa taactcttgt
     2341 cgaaaaagcc agcaagctga aagacaggta ttcaccgcca ccagcgcgtt taacgtaccg
     2401 caccgctttt cgggcataaa aaaaccgcct ggcggcgggt ttttctttac tttgccatcg
     2461 catacaaaat cggcaaaata tcagatttac acgaaacata cgcgatttaa ttgacttttg
     2521 caatatctcg tcgtgaaaag gttgcttttt gttgcgatct cattttcaca gtgcaaatta
     2581 aggattctgt atcgagattc ttaaaaatgt cgcacatctc acgccagtag ttcgcataat
     2641 tatggctcca gttatcaggc ttaattccac acagtctggc aagctccagt cgctggtaga
     2701 cctcacgccc aataacccat cccctgacat cctgtgccgc cagccagatc aacttcttca
     2761 cgcgctcctg cgtttttcct gcaatttttc tggtgccgta ccgggtttta aattcattcc
     2821 acacccactg cgttatcgcg atctgatgct cccagcaaat atttccgttg taacaccaca
     2881 acaaccaggc tttatgatgt tcttcaagag acagaacggc ccgccgccat gatgatgtcg
     2941 aaaactcagc cggactgacc agggcaattg atgagccttt cgccagcgat tgttttcccg
     3001 ggatcggtgg attatcccgc gttatcattt ttccggttac ttcatcgcgg taccggattt
     3061 ttttgcgtct gtaacgccct gtatcgaaca gcgcattctc ctgccaggct tcgagctggc
     3121 cttttgtcga accagtgaga tccgcagtgg caatcatgag ttgctcgcga acaaactgta
     3181 aatactggtt attcatgcac tccccacctc tgtaattttt atctccagtc gtccaccaga
     3241 tactggccga ccacgtacaa tattgatttc atcaaactgc tcgtcatcaa tgagcactcc
     3301 cgcatgcgtc agcgcatcca gcggcgcttt cagaatattg tccaggtcac gacggcgctt
     3361 atccggtggc tctgcaatca cctttatcgc cagccttccg gacaggttta atttcagccg
     3421 ctgctggcga acaataagcg ccacagcccg gcgataacgc tttccctcct ccgagataaa
     3481 atatgtactg ccacgacgac gccagtaggt gttcaccgtc ggcgggtaag gtaaaaccaa
     3541 atctatgagc atcagtcacc tcttttaccc gagcacgcca gttgcaaagg cgtgatcaag
     3601 aaaacgaaaa attaactcaa cctgggagcc gtactttttc tcaaacgcca gcgggtcagc
     3661 atgaagttcg ttgtggtgct cccggcacaa cggtagtgtg aaaatatcgt gggattttgt
     3721 ccccataccg ccctgcccat ggccaatcag gtgatgcggc tcatcagctg gctttccaca
     3781 gcatgcacac ggctgcgtct taacccagcg cgtgtacttt tcattaaccc agcgacgacg
     3841 tttaggccgt ttcatgaaag attccggaga ctccggatca acggcgatac tgaccaccgc
     3901 ctttttctgt ggtggatttt gttgctggtg gacgtgaggt ggcagcgcaa tattttttgt
     3961 gcgctgcttc agtatgctgg tggctgtctg ttctcccggt acgatgtcgc tctcactgta
     4021 tacggagcgg attttttcca ccggtaatcc cagcgaacga cgcaatacag actccggaat
     4081 tgcatcagcc acctgatgac aaaccgccca ccagcataac tctgccagcg acagttcccg
     4141 ctcctgcgtg ccattcattg catgacggat gacgtcaatc atccaggcag ccagattctg
     4201 ctgagcaagt tgctccagtg aatcagatgt ctgctcccgc agctggttgt cgcagtgcca
     4261 gcacaacacc atcgcgccgg taccgtaacg gtgaatgacg gtttcgctat gatgataatc
     4321 accgtgtggc cactggcagg atttcacgtg acgtaataac cagtcagaca gtgcaccagc
     4381 gccacccgca gcacggttta cccgctcatc actgaaaaat ggcagtaatg atttgtcttc
     4441 caccagcggc tggcgaacag tgggaaccgc tccggatggc agcgcccgca tgtttttcgg
     4501 ttccggctcc accagcaccc tgccgcaatg aaaaaccggc agtgattcac gaccgggcgt
     4561 aaggaccacc agcccgagtt ccggaaccag aacaggtcga agtaataccc gcacattacc
     4621 tccagacgcg ctgacggtag caggcatgtg tccgtggcag atgtgcacga acaggaagat
     4681 atacagaaac ggtccaggtc agacggtcag cgttcaggct ccgctccaca cggacaccgc
     4741 gacgccaata cgcctgttga agcatatcgg cctcatcggt cgaacagaac agataatgaa
     4801 accagccata ctgaggcaca cggaaacgcc gcccctgctt agtagtcggg gcggcttcgg
     4861 aattgtggga ttttatatgt tgcgccatcg gcttctccgg tgacgcagca ggtgccagtt
     4921 gttcaggctg acggcgcgaa ttgtaaggca atacgccgga atgtacaaac agaaaacccg
     4981 tcagtaagac ggggataacc agaaccggtt actttaataa tttcagcgtt tttaggtaat
     5041 gactc
//
LOCUS       DAJSKJ010000214         5015 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000214 DAJSKJ010000000
VERSION     DAJSKJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.218"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            103..693
                     /locus_tag="ON345_003556"
     CDS             103..693
                     /locus_tag="ON345_003556"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCR3073554.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLINMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(775..1515)
                     /gene="pflA"
                     /locus_tag="ON345_003557"
     CDS             complement(775..1515)
                     /gene="pflA"
                     /locus_tag="ON345_003557"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR3073555.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1707..3989)
                     /gene="pflB"
                     /locus_tag="ON345_003558"
     CDS             complement(1707..3989)
                     /gene="pflB"
                     /locus_tag="ON345_003558"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3073556.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4044..4901)
                     /gene="focA"
                     /locus_tag="ON345_003559"
     CDS             complement(4044..4901)
                     /gene="focA"
                     /locus_tag="ON345_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR3073557.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1282 a         1189 c         1250 g         1294 t
ORIGIN      
        1 cattagtgaa aatccttcat taactcatcc tgaatcatca cgtttacagg attatgccgc
       61 ctgcgtaaag tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc tgaacgtatt
      121 tatttggtat gggcccatcc tcgtcatgat tcattgaccg cacatattgc tgatgcgatc
      181 catcagcggg caatggagcg gaaaatacag gtgacggaac tcgatttata tcggcgtaat
      241 ttcaacccag tgatgacgcc ggaagatgaa ccagactgga agaatatgga taaacgttat
      301 tctccagagg ttcatcagct ttattcagag ctgcttgaac atgacacgtt agtggtggtt
      361 tttcctctct ggtggtacag cttcccggca atgctaaaag gatatattga cagagtatgg
      421 aataatgggc tggcttatgg agatgggcac aaattaccat tcaataaagt tcgttgggtg
      481 gcgctggttg gaggagacaa agaatcattt gtccagatgg gctgggaaaa aaatataagc
      541 gattatttga taaatatgtg cagttatctt ggtattgaag atgccgatgt cactttcttg
      601 tgtaatacag tggtattcga tgggcaagaa cttcatgcga gctattatca gtcgttatta
      661 tctcaggtac gggatatggt agatgcacta taaaatgtgt taaaaacgct gtagcagaat
      721 gaagcgcgga ataaaaaagc ggcaactcaa taaagttgcc gctttacggg gaaattagaa
      781 cattacctta tgaccgtact gctcaagaat gcctttcacg cgttccatgg tctctttctt
      841 cggtggttta acaccgtcga gtttgtactc ttcacccatt gccacccatt tgtgtttgcc
      901 cagctcgtgg taggggagaa gctcgatttt ctcaacgttg cccatatcac gggtaaattc
      961 accgaggcga tgcgctgaat cgtcatcgtc agaccagcct gggacaacaa cgtagcggat
     1021 ccacaccttc acatttttgt tcgccagata tttggcgaac tccagcgtgc ggtgattgga
     1081 aactccaacc agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag
     1141 gtcggttact tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt
     1201 gtccagacag gtatgaatgc cttctttttt gcaggcgcgg aaccagtcac gaacaaactc
     1261 agcttgtagg attgcctcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa
     1321 gtggcgatag gtcaccacct ctttcattaa atcttcaacg gtaacttctt taccgccatg
     1381 cgtatcccag gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa
     1441 ggtgataaag cgaatacctg ggccgtctac ggttccacag gattcaaagg agtgaatgcg
     1501 accaataact gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc
     1561 gcagctcgaa ggctacgtcg agtctgtttt ggcagtcacc ttaaagtata gatagctgac
     1621 aaaaaaggct ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt
     1681 tactgtacga tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg
     1741 tcctgctgct gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg
     1801 gtcagctgcg gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc
     1861 acgttaacgt tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc
     1921 atcagaccag ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg
     1981 atagagaagg tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa
     2041 gtcagagacg ctacagcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg
     2101 aacggcgcgc cagcacgacg accgtctggg gtgttaccag ttttcttacc atacacaacg
     2161 ttagaagtga tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc
     2221 tgaattttct tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatca
     2281 ttgttaccaa actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg
     2341 tcacgaatcg gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac
     2401 agaccagcga taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga
     2461 gaggcttcgt agctgtactt gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac
     2521 tgtttagcca gccagtccat gaagtgatcc atgcgctcca tcacttcatc atagttcagg
     2581 acatcgcctt tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg
     2641 ccgccgttga ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc
     2701 atttgtttac caacgatcat cgggcttacg cagcaagcaa tagcgtagtc atcgttgttg
     2761 aagtccggac gcatcagatc atcgttctca tactgcagag aagaggtgtc gatggacact
     2821 ttagcggcga atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc
     2881 ggttccggag acggacccat ggtgtacagg gtgttcagga aacggaagct gtttttggta
     2941 accagggtac gaccgtcgag gcccatacca ccgatagatt cggttgccca gatcgggtcg
     3001 ccagagaaca gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg
     3061 accaggtggt caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca
     3121 cgttcgatgt acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc
     3181 tgagacttaa cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg
     3241 gtagccggac cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga
     3301 gcgcggtgct gttcagcgat ttcttcgcgc agacggatag tctgttccag gtttacgccg
     3361 ttttccagat cagcctgcag agaagtgaac tgtgccagtt tgtctttcat caggtagtcg
     3421 ataccgtaca gcgcaacgcg acggtagtca ccgatgatac ggccacggcc atatgcatct
     3481 ggcagaccgg tcagaacacc agatttacgg caacgtagga tgtccggagt gtaaacgtcg
     3541 aacacgccct ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcggatcc
     3601 agttcgcggt tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata
     3661 agagcacgtt tcagcggagc ttcagtctgc agaccaacga ttttctcaag ctgcttgttg
     3721 atgtagccag cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa gtcaactggc
     3781 gcgtgagtgc ggttttccag tttaacgcct tccattactt tgtcccacag ggtggtggtc
     3841 gcttcagtag cgccagccag gaaggactcg tcaccctcgt acggagtgta gtttttctga
     3901 atgaagtcac ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct
     3961 gtggctaact tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta
     4021 tttactgcgt acttcgacaa ccattaatgg tggtcgtttt cacgcaggta aatgacccag
     4081 tatgtcaacc caaccaacaa accaccaccg ataatgttgc cgatcgtaac cggaatcagg
     4141 ttatcagtga tgaaattcat cactgtcagg tgagaaaaat tttccggtgc agaaccgact
     4201 gcggtccaaa attccgggga tgcgaagtcg cggattacaa tacccatcgg gatcataaac
     4261 atgtttgcga tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata
     4321 atgaacgctt tgtccatcag gctgcggcca gaataactca tccatactgc cagacatacc
     4381 atcaggtttg ccaggatacc aagacagacg gcctcaataa aagtatggtg cactttgtgg
     4441 tcggcggttt gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa
     4501 agccacatta aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc
     4561 cagtttttcg ccaactgacc ccaggtgatg cgcccactcg ccttagcaac aacaatcaac
     4621 acggtggaag taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag
     4681 caaatgccgc caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct
     4741 gtgatataga agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa
     4801 gtcttaagcg gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt
     4861 gcagcaggaa gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta
     4921 agtcggcgac gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc
     4981 gcctggctta taggcccgta actcgcatgg ttttt
//
LOCUS       DAJSKJ010000215         5002 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000215 DAJSKJ010000000
VERSION     DAJSKJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.219"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..260
                     /locus_tag="ON345_003560"
     CDS             <1..260
                     /locus_tag="ON345_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR3073558.1"
                     /translation="MDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGW
                     NPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(380..559)
                     /locus_tag="ON345_003561"
     CDS             complement(380..559)
                     /locus_tag="ON345_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR3073559.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            633..1994
                     /gene="pabB"
                     /locus_tag="ON345_003562"
     CDS             633..1994
                     /gene="pabB"
                     /locus_tag="ON345_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR3073560.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAGQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            1998..2576
                     /locus_tag="ON345_003563"
     CDS             1998..2576
                     /locus_tag="ON345_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR3073561.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2760..4124
                     /gene="sdaA"
                     /locus_tag="ON345_003564"
     CDS             2760..4124
                     /gene="sdaA"
                     /locus_tag="ON345_003564"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR3073562.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYTGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4255..>5002
                     /locus_tag="ON345_003565"
     CDS             4255..>5002
                     /locus_tag="ON345_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR3073563.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAV"
BASE COUNT         1199 a         1275 c         1277 g         1251 t
ORIGIN      
        1 ccatggatgt atcagcgcca acaccaagtt cgatgaatga aagcacggcg aagggaatct
       61 ttaaatattt aaaagaactg ggagtacccg cgagtgccgc tgatattacg gcgcgagctg
      121 accaggaagg ctggaaccct gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg
      181 agactggtga acgctctgtg attaaaaatc ctgaatactt ttcaacatat atgcaggaag
      241 aactaaaagc actggtctga gttaaattta tatcagcata aatgggtcag gacgctttta
      301 atcacatata aaaagcgtcc tttttctcac aatcaaataa tcatcggctt ctataacgaa
      361 atcgatacgg attagtgtgt tactcgtctt catcctcaaa acgcgccacg attcgctcgc
      421 cggaatggtt ggcacgcagc tcttctgcca ccagcgcgat tgcctggccg ctgctcatcc
      481 cttgtgccat cagctcctgg atccgctcga cggctttttg ctgctgttca tgggtgagtg
      541 aaggtaaacc tgcaaacatt gttaactcct gctaaattgt tggcgctaat tatttcatgc
      601 tacccggcac atagccagta gagtcaggac tgatgaagac gttatctccc gctgtgatta
      661 ctttaccctg gcgtcaggac gccgctgaat tttatttctc ccgcttaagc cacctgccgt
      721 gggcgatgct tttacactcc ggctatgccg atcatccgta tagccgcttt gatattgtgg
      781 tcgccgagcc gatttgcact ttaaccactt tcggtaaaga aaccgttgtt agtgaaagcg
      841 aaaaacgcac aacgaccact gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg
      901 cagacattcg cccaacgcat aacgaagatt tgccatttca gggcggcgca ctggggttgt
      961 ttggttacga tctgggccgc cgttttgagt cactgccaga aattgcggga caagatatcg
     1021 ttctgccgga tatggcagtg ggtatctacg attgggcgct cattgtcgac caccaacgtc
     1081 atacagtttc tttgctgagt cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc
     1141 agcaattctc gccgcaggaa gatttcacgc tcacttccga ctggcaatcc aatatgaccc
     1201 gcgagcagta cggcgaaaaa tttcgccagg tacaggaata tctgcacagc ggtgattgct
     1261 atcaggtaaa tctcgcccag cgttttcatg cgacctattc tggcgatgaa tggcaggcat
     1321 tccttcagct taatcaggcc aaccgcgcgc catttagcgc ttttttacgt cttgaacagg
     1381 gtgcaatttt aagcctttcg ccagagcggt ttattctttg tgataatagt gaaatccaga
     1441 cccgccctat taaaggcacg ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag
     1501 cagaaaaact ggcgaactca gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa
     1561 tgcgtaatga tatcggtcgt gttgccgtag caggttcggt aaaagtacca gagctgttcg
     1621 tggtggaacc cttccctgcc gtgcatcatc tggtcagcac cataacggcg caactaccag
     1681 aacagttaca cgccagcgat ctgctgcgcg cagcttttcc tggtggctca ataaccgggg
     1741 ctccgaaagt acgggctatg gaaattatcg acgaactgga accgcagaga cgcaatgcct
     1801 ggtgcggtag cattggctat ttgagctttt gcggcaacat ggataccagc atcactatcc
     1861 gcacgctgac tgccattaac ggacaaatat actgctctgc gggcggtgga attgtcgccg
     1921 atagccagga agaagcggaa tatcaggaaa cttttgataa agttaataag atattacgcc
     1981 aactggagaa gtaagacgtg gaataccgta gcctgacgct tgatgatttt ttatcgcgct
     2041 ttcaactttt gcgcccgcaa attaaccggg aaaccctaaa tcatcgtcag gctgccgtgt
     2101 taatccccat cgtccgtcga ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc
     2161 tgcgtaaaca cgctggacaa gtggcattcc ctggaggtgc agtcgatgac acggacgcat
     2221 cagttatcgc cgccgcgctg cgcgaagctg aagaagaggt cgctataccg ccttccgccg
     2281 ttgaagttat cggcgtgctg ccgcccgtcg atagcgtaac tggctatcag gtaaccccag
     2341 tggtcggcat tatcccgccc gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg
     2401 tgtttgaaat gccgctcgcc caggcattac atctgggtcg ttatcaccct ttagatatct
     2461 accgccgtgg cgattcacat cgggtatggc tgtcctggta cgaacagtat tttgtatggg
     2521 gaatgaccgc aggcataatt cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat
     2581 acttatcttt acatctacaa aacactactt gagacaatca tcgcaatatt agttaaatcg
     2641 cggtttttga ttagtttaat tcatgtgaat agttaagcca gtcgccgcgt tccctcttac
     2701 actatgcgct gttattagtt cgttactgga agtccagtca ccttgtcagg agtattatcg
     2761 tgattagtct attcgacatg tttaaggtgg ggattggtcc ctcatcttcc cataccgtag
     2821 ggcctatgaa ggcgggtaaa cagttcgtcg atgatctggt cgaaaaaggc ttactggata
     2881 gcgttactcg cgttgccgtg gacgtttatg gttcactgtc gctgacgggt aaaggccacc
     2941 acaccgatat cgccattatt atgggtcttg caggtaacga acctgccacc gtggatatcg
     3001 acagtattcc cggttttatt cgcgacgtag aagagcgcga acgtctgctg ctggcacagg
     3061 gacggcatga agtggatttc ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc
     3121 cgctgcatga aaacggtatg caaatccacg cctataccgg cgatgaagtc gtctacagca
     3181 aaacttatta ttccatcggc ggcggtttta tcgtcgatga agaacacttt ggtcaggatg
     3241 ctgccaacga agtgagcgtg ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact
     3301 gtaatgaaac cggctattcg ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca
     3361 gcaagaaaga gatcgacgag tatttcgcgc atgtctggca aaccatgcag gcatgtatcg
     3421 atcgcgggat gaacaccgaa ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt
     3481 ctgccctgcg ccggatgtta gtttccagcg ataaactgtc taacgatccg atgaatgtca
     3541 ttgactgggt aaacatgttt gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg
     3601 tggtaactgc gccaaccaac ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg
     3661 accactttat tgaatcggtc agcccggaca tctatacccg ttactttatg gcagcgggcg
     3721 cgattggtgc attgtataaa atgaacgcct ctatttccgg tgcggaagtt ggttgccagg
     3781 gcgaagtggg tgttgcctgt tcaatggctg ctgcgggtct tgcagaactg ctgggcggta
     3841 gcccggaaca ggtttgcgtg gcggcggaaa ttggcatgga acacaacctt ggtttaacct
     3901 gcgacccggt tgcagggcag gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg
     3961 tgaaggcgat taacgccgca cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc
     4021 tggataaggt catcgaaacg atgtacgaaa ccggtaagga catgaacgcc aaataccgcg
     4081 aaacctcacg cggtggtctg gcaatcaaag tccagtgtga ctaatacttc ttactcgccc
     4141 atctgcaacg gatgggcgaa tttatacccg ctttctcgtc tgctgtaata ttccccacta
     4201 cacttccact gttgcgtcag gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa
     4261 aaagcacaac ggatcattaa aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc
     4321 gcactcgtta cgctcgcttc gaccctgagc gtgcgattta tttcacagcg taacttaaat
     4381 caacaacggg tagtacaatt cgccaatcac gctgtagagg aattagataa agtactgctt
     4441 cccctacagg caggtagcga agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc
     4501 catttgccat tacgtaaaca ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg
     4561 caagacggca cactttattg ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac
     4621 attctggctg aacttcctgc accgcaacca cttttacgcc tgacgatcga ccgtgccctg
     4681 attaaaggca gtccggtttt gattcaatgg acgcctgcag cgggcagtag caatgctggg
     4741 gtcatggaga tgattaacat cgacttactg acggcaatgc tccttgagcc acaactgccg
     4801 caaatcagta gcgccagcct gacggtggac aaacggcatt tgctctatgg taatgggctg
     4861 gtagattccc ttccgcaacc tgaagacaat gaaaactatc aggtttcttc gcaacgcttt
     4921 ccttttacca ttaacgttaa tggtccgggg gctacggcgc tggcatggca ctatcttcca
     4981 acacaattac cgctggcggt gc
//
LOCUS       DAJSKJ010000216         4991 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000216 DAJSKJ010000000
VERSION     DAJSKJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.220"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(61..996)
                     /gene="ldtA"
                     /locus_tag="ON345_003566"
     CDS             complement(61..996)
                     /gene="ldtA"
                     /locus_tag="ON345_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR3073564.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(1058..2137)
                     /gene="cobT"
                     /locus_tag="ON345_003567"
     CDS             complement(1058..2137)
                     /gene="cobT"
                     /locus_tag="ON345_003567"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR3073565.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEKGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(2149..2892)
                     /gene="cobS"
                     /locus_tag="ON345_003568"
     CDS             complement(2149..2892)
                     /gene="cobS"
                     /locus_tag="ON345_003568"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR3073566.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVLSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(2889..3434)
                     /gene="cobU"
                     /locus_tag="ON345_003569"
     CDS             complement(2889..3434)
                     /gene="cobU"
                     /locus_tag="ON345_003569"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR3073567.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         1354 a         1340 c         1053 g         1244 t
ORIGIN      
        1 agccaaattc aaaaaagcct gctttcgagc aggctttttg ctttctaatt accaacgctc
       61 ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc gacgttgcaa
      121 cgcagcattt gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg
      181 ggtcaccggc aatggcactt ttcggtcaga ctcatattct gcacgattgc gcgacagcgg
      241 ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc
      301 aataatttgt acacgagtgc ctacaggaac attatcaaac agatatttga tatcgtcatt
      361 acgcagacga atacagccct gacttacacg gagcccaata ccaaaattgg cattggtacc
      421 gtggatggca tacaacctgc caatataaat cgcgtacagc cccatggggt tatcgggccc
      481 cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg
      541 cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg
      601 ggtttctcgc ccggcctggc cgataccaat aggaaagact tccactgtat tactgtctgg
      661 cggatagtaa taaagacgca tttcagcgac gttaaccaca atccctttac gaacagtggc
      721 gggcaaaatc agttgctgcg gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc
      781 agcgcccgga ttcgcttcca gcatattact taacccttgc ccgtattgtg cggcaaaagt
      841 ctccagcggc tgagtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg
      901 gctaccctct ggaggtaatg gataagttac cgccaggcta ttttggctgg caaaaagcag
      961 agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaatac
     1021 tatccgataa gtatagcttt tatcagactt ttcgttttta gctgttcaaa tcagaagtcg
     1081 tattccccgg tagaacaata ttactggcag caagttcgcc catgttgttg tatatcgcac
     1141 aagcagcttc gatgatggac atcgccagag ctgcgccact cccctcacct aaacgcatat
     1201 ccatattgag ataaggctcc agccccaaat gcgagagcgc tatacgcgcg cctttttctg
     1261 ccgacaagtg agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga
     1321 gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcac
     1381 ctaacatcac tccagctatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa
     1441 caccatcctg aggatttggt tgattcaacg taatcgcccg acgcacaaca tcaattttat
     1501 tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc
     1561 cagtgattgt gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta
     1621 caccaaacag cgtgacaccg ttttttgcca gctcccgcgt ataacagatg acgtccaaaa
     1681 gcaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc
     1741 gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa
     1801 ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccacggg
     1861 tcatattttc agcctgtatg gctgtcactt cttttggaga aatagcgacc cctttctccc
     1921 agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat
     1981 tcaaccccgg cattcctgcc agttgtatgg caagcgcctc cagctttcct aggctaccaa
     2041 caggtttgag taacccgtca acatgccgtt gtgcacgcga catagcggca gaattgatgg
     2101 ctgggatcgt attcagtaaa tcggcaagta tttgcatgtc acgtctcttt atagcagtgc
     2161 cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc ccgtttgccc
     2221 gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat
     2281 agcagccaca ccatgcatac ccggcaataa tacggcagca aaaatagcgg ctaagccgag
     2341 tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac acattgccaa gcccttcttc
     2401 ccgcgcataa cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc
     2461 tgccgccagc gatgcaagga tcggttcgcc acgcagagcc aactcgctta ataccagaat
     2521 ctttgccagt acaacaaaaa tcaatgccag accgccatgg gtgcctaaac ggctatcacg
     2581 catgatttcc aacatgcgat cgcggctacg tgcagacaat acgccatcac aggtatcggc
     2641 gaggccgtcc aggtggaatc ccccggtcat cagcgccagc acaagtacgc taaacagtgc
     2701 cgccagtggt acgccacacc atgcctgcag caccatgaag accagcccgc taatcgcgcc
     2761 aagcaataat ccaattaaag gaaaagtaat aataccgcga gaataatgct cgaaatccag
     2821 tccctgggac cagcgacgcg gtacaggcag acgcgtaata aatgagagca tcgcccaaaa
     2881 taatttactc atttgatttt tactccaata cccgaaacca ccagccatac ttcatttgcc
     2941 gcagcggcca actgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga
     3001 ctctccggca caatccccat tcccacttcg ttagtcacta atacaacctt tgcggggcaa
     3061 cgttggcagg cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc catcgcctga
     3121 taatcccatt catcagggtc tttatcgccg ccataatcaa acaacagatt agtcaccatt
     3181 gtggtaacgc attccagcaa cacaacctca ttcgggttaa tgtctgcatg aattaattca
     3241 tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg ggcggccttg ccgatgatgt
     3301 tctatccgtg cagccatctc atcatcaagg atttgcgagg tagcgatata cagaacctgt
     3361 gaagagtccc caataagcgc ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc
     3421 accaaaatca tcataccgac tcctgattca aatgtatgat ggtattcatt gaacagaaca
     3481 atggatcccc caccgttgct gaccgcaaaa catttacgcg attaatactg cgcgtaatat
     3541 aattaaaaat ccagcattct caatccatta cgaaataaga tggcattacg gaatagccag
     3601 actttctcgt gttaacgtcc gacaaaatgt gtcattgaca gcggtttctg tggattgaca
     3661 catttcattc agataatgaa ttaatgctac tgccggacca accagcaaac gggcattgtg
     3721 ttctgaaatc caggctattg attcaacgtc agcgacagat ctgcgctgta aaagaacttg
     3781 taaaacaacg caatagaatt atccggaatg gtggcgacta tgcactagaa caccacaatt
     3841 tcgctctctc ggtaaggcac tgtcacagca ttcaacagaa tgtgacttgc cagatttatt
     3901 agcgccacca gatgtttaac cgggtaacca cgacccagtc ggggatatat tccatcttct
     3961 cgctgacatc atcaagaata aaaaggttta tcacactcag agcaacgttc ttcagcaggc
     4021 gggataattc ttcaacattt aaaagatgtg ttattaaagg catacaaact ggacgtattg
     4081 tattttcttt tggtggttga cctaaggtag cagtttatcc tgatgcgctg agatttctgt
     4141 aatatctacg tcaacattct cctcgaatag aaaatgcagc cccggcaagt gattcacatt
     4201 ttttaacaaa acgttgttag cgtgccattt tcagaagctc tccaagaaag cgaattcttt
     4261 tctcaaattc tgcattctca gacacagctt tctactccat ggcaatggcc caaatacgaa
     4321 gttgctcagg atcattagtg gtgctgagaa gttaaataac catgcggagt accactcgtc
     4381 atcacgtatt tatatatcat gaagctattg ttgtgttttg tacatcagta gatgtattct
     4441 gatatactcc ttttgctaga cataaccttt cacctgcttg caaagcttct gtgttctgac
     4501 attgccaaat tgttgcaatt ctgtatccag ccttctttca gtcatagctt cgggccgcga
     4561 taagactcac tgatctgacc ctgattcctc ttgcagactt tatagaccaa ttaaaatgca
     4621 gtttctgcag gtcaacgtct gaccatcatt gtcatcactc tggccattag agtaaccttc
     4681 tgcattcatc cttttgtaaa aagtttatag actggccccc tgaatctcca gacaaccaat
     4741 atcacttaaa taagtgatag tcctgataca agtttttaga gtagtcatta gtgaacagat
     4801 aattgttaac ttagaaccag taaaatgcaa acgacgcacc acgctgggaa aatcgctgct
     4861 attcagcaga gttttgtacc gagaattgtg gccagccact tattgctctc gcgtaagcag
     4921 gtatccccat cgtttaccag tccagcacat cttgtggtca ggcagaagaa gagcaaactg
     4981 ttactggact c
//
LOCUS       DAJSKJ010000217         4987 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000217 DAJSKJ010000000
VERSION     DAJSKJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.221"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(22..480)
                     /locus_tag="ON345_003570"
     CDS             complement(22..480)
                     /locus_tag="ON345_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR3073568.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            600..1409
                     /gene="proC"
                     /locus_tag="ON345_003571"
     CDS             600..1409
                     /gene="proC"
                     /locus_tag="ON345_003571"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR3073569.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1426..2541)
                     /gene="adrA"
                     /locus_tag="ON345_003572"
                     /gene_synonym="dgcC"
     CDS             complement(1426..2541)
                     /gene="adrA"
                     /locus_tag="ON345_003572"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR3073570.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWRWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2643..2963)
                     /gene="psiF"
                     /locus_tag="ON345_003573"
     CDS             complement(2643..2963)
                     /gene="psiF"
                     /locus_tag="ON345_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR3073571.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3082..4497)
                     /gene="phoA"
                     /locus_tag="ON345_003574"
     CDS             complement(3082..4497)
                     /gene="phoA"
                     /locus_tag="ON345_003574"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR3073572.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4598..4858)
                     /gene="iraP"
                     /locus_tag="ON345_003575"
     CDS             complement(4598..4858)
                     /gene="iraP"
                     /locus_tag="ON345_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR3073573.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1168 a         1348 c         1224 g         1247 t
ORIGIN      
        1 agtgtaaata ataaattaca tttagccacg actacgttgc acttccagcc accacttctc
       61 cagctccgcg gcaaaggcct ggcggtcacg ttgtgaaagg ctatctggtc cgccggtctg
      121 gatcccactg gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct cacgaatggt
      181 cgctggcgtg taacgttcgc cgcgcggatt aagcgccgct gcgcctttct cgatggcttc
      241 agcagccaaa ggtatatctg cggtgatcac caaatcgccc gcttcacact gccggacaat
      301 ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa atcgcgatgg
      361 cggcacgcgt aaactctggt ttgctaccag taccagcggc atctgcatac gttccgccgc
      421 gcgatacaaa atctctttaa ttacattggg acacgcgtcg gcatccaccc aaattgtcat
      481 aaagtcatcc tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc cggcatagtt
      541 ctgtttatgc ttctgccagc gattatcaaa acaatgaatt tcacggcagg agtgaggcaa
      601 tggaaaagaa aatcggtttt attggctgcg gcaatatggg aaaagccatt ctcggcggtc
      661 tgattgccag cggtcaggtg cttccagggc aaatctgggt atacaccccc tccccggata
      721 aagtcgccgc cctgcatgac cagttcggca tcaacgccgc agaatcggcg caagaagtgg
      781 cgcaaatcgc cgacatcatt tttgctgccg ttaaacctgg catcatgatt aaagtgctta
      841 gcgaaatcac ctccagcctg aataaagact ctctggtcgt ttctattgct gcaggtgtca
      901 cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa aattatccgc gccatgccga
      961 acactcccgc gctggttaat gccgggatga cctccgtaac gccaaacgcg ctggtaaccc
     1021 cagaagatac cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg gaagtaattg
     1081 ctgagccgat gatccacccg gtggtcggtg tgagcggttc ttcgccagcc tacgtattta
     1141 tgtttatcga agcgatggcc gacgccgccg tgctgggcgg gatgccacgc gcccaggcgt
     1201 ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa acgggagaac
     1261 atccgggggc actgaaagat atggtctgct caccgggagg caccaccatt gaagcggtac
     1321 gcgtactgga agagaaaggc ttccgtgccg cagtgatcga agcgatgacg aagtgtatgg
     1381 aaaaatcaga aaaactcagc aaatcctgat gactttcgcc ggacgtcagg ccgccacttc
     1441 ggtgcggtta cgtccggctt tctttgcttt gtaaagcgcc aaatctgccg atttcaacca
     1501 ctcacgatag tgactcattt gtgggttcag cggcgcaacc cccacactaa tccgtaaagt
     1561 tacctgtggc gtattcggca agcgtaacgt gtttagccct tcacgcaccc gtaacatggc
     1621 ggtaatggcg ctctcagctg gcgtaccgga catgattact gcaaactcat cgccgccaaa
     1681 ccgaccaatc acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa gcgccacaat
     1741 cgcttcatcg cccacatcat gaccccaggt atcgttgatg ctcttgaaat ggtcgatatc
     1801 gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt cattgcgtaa
     1861 catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcggg tactcatgac
     1921 ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga cccagccaaa
     1981 cagcagagga taaacgacaa ttatgggaag ggagagccac cattccagcg gcgcactatt
     2041 gaacgacacg gtaatgcccg taagctcgag ggtgacaagg caggaaacca ccatcaacac
     2101 cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac acataatcat
     2161 caacatagcg gtggaaggca gcacgtttac gcccattacg cctacccaca ttcccgctaa
     2221 tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg ccctgctcgc
     2281 tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacaaca gccacctcca
     2341 gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc cagccaggcc
     2401 aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct gatgttcgtt
     2461 ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa agttttcatc
     2521 attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga cggggctaaa
     2581 ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag cactatcagg
     2641 tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc acctttcaga
     2701 gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg cgtcaaactt
     2761 ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt cttacgagca
     2821 tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg ctgttgttgt
     2881 ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc aaaaagcaag
     2941 gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc atgagcgttt
     3001 gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc cggcagcgaa
     3061 aattcactgc cgggcgcggt tttatttcag ccccagagcg gctttcatgg tgtagaagag
     3121 atcggtctgg tcggtcagtc caacaacatt ggcggcatgc gggccatacg ccgcaatacg
     3181 caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac tcatcaccat
     3241 cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg tatccggcgc
     3301 aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt taccctcctt
     3361 tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct cgccaatttg
     3421 cccacaagga ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa cttgcaggaa
     3481 aaatcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct gtgccagggt
     3541 tggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct tatcgatatt
     3601 gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt cagcaaacag
     3661 gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg cagcatcgtt
     3721 caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct gccattcacc
     3781 agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt cggcacgagc
     3841 gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt tacccggaca
     3901 tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg ccaccagcgc
     3961 agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca gacctgcggc
     4021 ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc ccagcgcgcc
     4081 gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt cggtgacgta
     4141 gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg taagcggtaa
     4201 ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg cggcagtaat
     4261 ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag gtttatcgct
     4321 aagagaatca cgcagagcgg cggtctgatc acccgttaag cggcgagcac cgccgggtgc
     4381 agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg tccgggcttt
     4441 tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt gtttcacttt
     4501 attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa gtctcggccg
     4561 tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga tgcttcaata
     4621 acttctttac gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg gcatcgggct
     4681 ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca ttttgcgcca
     4741 tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca cacagttctt
     4801 tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga tttttcatgt
     4861 ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac ttcatagtgt
     4921 ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt tgtttgatta
     4981 ccagcga
//
LOCUS       DAJSKJ010000218         4896 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000218 DAJSKJ010000000
VERSION     DAJSKJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.222"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            209..2359
                     /gene="btsT"
                     /locus_tag="ON345_003576"
     CDS             209..2359
                     /gene="btsT"
                     /locus_tag="ON345_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR3073574.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2409..2612
                     /locus_tag="ON345_003577"
     CDS             2409..2612
                     /locus_tag="ON345_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR3073575.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2623..3579
                     /gene="yjiA"
                     /locus_tag="ON345_003578"
     CDS             2623..3579
                     /gene="yjiA"
                     /locus_tag="ON345_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR3073576.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLSLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(3625..4539)
                     /gene="mrr"
                     /locus_tag="ON345_003579"
     CDS             complement(3625..4539)
                     /gene="mrr"
                     /locus_tag="ON345_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HCR3073577.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            4727..>4896
                     /locus_tag="ON345_003580"
     CDS             4727..>4896
                     /locus_tag="ON345_003580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073578.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIP"
BASE COUNT         1118 a         1343 c         1266 g         1169 t
ORIGIN      
        1 tatcgtcact taaacgacgc ctttgccgct caaccgcaaa actgaccgct tacatcccta
       61 aaataaccac tcagttattt accttacttt acgcgcgcgt aactctggca acatcactgc
      121 aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc ttcggccaac
      181 tattaatcaa tacatgccag gttttactat ggatactaaa aagatattca agcacatacc
      241 ctgggtgatt ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg
      301 ggagcacgtc agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta
      361 tcgctactac agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac
      421 gcctgcggtt attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt
      481 tggtcaccac ttcgccgcta tcgccggtgc tggtccgctg gttggtccgg ttctcgccgc
      541 gcagatgggc tacctgcctg gcacgctgtg gctgctggcg ggggtcgtgc tggccggtgc
      601 ggtacaggac tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga
      661 gatgatcaaa gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt
      721 aatcatgatc atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag
      781 tccgtggggt gtcttcaccg tttgctcaac cgtaccaatt gcgctgttta tgggtatcta
      841 catgcgcttt atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct
      901 ggttgcctct atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact
      961 gacctttaaa gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcgct
     1021 gctgccggtg tggctgatcc tcgcaccacg cgactatctg gcaaccttcc tgaaaatcgg
     1081 cgttatcgtc ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc
     1141 gatgacccag tacattgacg gtactggccc gctgtggaaa ggcgctctgt tcccgttcct
     1201 gttcatcacc atcgcctgtg gtgcggtatc tggcttccac gcgctgatct cttccggtac
     1261 gacgccgaaa ctgctggcta acgaaaccga cgcgcgtttc attggctacg gcgcaatgct
     1321 gatggagtcc ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct
     1381 ttacttcgcg atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga
     1441 aatgggtggc gagaacgcgc cgatcatcat ggcgcagttg aaagacgtta ccgcacacgc
     1501 ggcggcgacc gtcagctcct ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc
     1561 gaaagacatt ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccga cgctggcggt
     1621 aggtatcgct cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca
     1681 cttcggtatt ctgttcgaag ccctgttcat cctgaccgcg ctggatgccg gtacccgttc
     1741 tggccgcttt atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga
     1801 ctctctggtt gctggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct
     1861 gtatcagggc gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat
     1921 ctccaaccag atgctggcag ccgtggcgct ggtactgggt accgttgtgc tgattaagat
     1981 gaagcgcacc caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac
     2041 cacctgggcg ctgggcctga aactgttcag caccaacccg cagatggaag gcttcttcta
     2101 catggcaagc cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat
     2161 tgccaacatg aaccacatcg ttgtgaacaa ctacaccaac gcaggtctga gtattctgtt
     2221 cctgattgtg gtgtacagca tcatcttcta cggtttcaaa acctggctga aggtgcgtaa
     2281 cagcgacaaa cgtactgaca aagaaacacc gtacgttcca atcccggaag gcggcgtgaa
     2341 gatctcttcg caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag
     2401 agtgaactat gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga
     2461 tgttgattgg cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg
     2521 acaagccgta catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg
     2581 gcgatggtaa aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt
     2641 accctactca ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac
     2701 gaacaacacg gctacaagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat
     2761 gatcaattga ttggtgatcg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc
     2821 tgttcgcgct ccaacgagct ggaagacgca ctactcgacc tgctggacaa tctcgacaag
     2881 ggcaatattc agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg
     2941 attattcaga cctttttctc ccatgagatt ttatgccagc gctacctgct ggacggcgtg
     3001 attgcgctgg tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag
     3061 tcccaggtgg gctacgctga ccgtattctg ctgactaaaa ccgacgtcgc aggcgaagca
     3121 gagaaactgc gtgaacgcct ggcgcgcatc aacgcccgtg caccggtcta caccgttacc
     3181 cacggcgaca tcgacctgag cctgctgttc aacaccaacg gttttatgct ggaagagaac
     3241 gtcgtcagca ccaaaccgcg tttccacttt atcgcggata aacaaaacga tatttcgtcg
     3301 attgtggtgg aactggatta cccggtagat atcagcgaag tttcccgcgt gatggaaaac
     3361 ctgctgctgg agtcggcgga taaactactg cgttacaaag ggatgctatg gattgacggc
     3421 gaacctaatc gcctgctgtt ccagggcgtc caacgcctct acagcgccga ctgggacagg
     3481 ccgtggggcg atgaaaaacc gcatagcacg atggtgttta tcgggattca gctgccggag
     3541 gatgagattc gggcggcgtt tgcggggtta aggaagtaag gggttatggg ccggataagg
     3601 cgcagccgca tccggcctga tatttcactc aaaatagtcc atatccagtt tcggcacctt
     3661 caacaaacgt gaagaaaccc ctacttcgtt ttcgatcatt aagtgcacca ggcgttcccc
     3721 atcaaccaac accataccct cgacggattg ggcaaagtca cgcgcctgag aagtaaatcc
     3781 agaagtggta ataaacaccc cacgtttcgc tttttgccca gccagtgcgc cgtaaaatgc
     3841 ctgtaattct ggcctgccta cagtattctg ccaacgtttt gcctgaacat aaactttctc
     3901 caggccaagt ttatcaagcg atatcacacc atcgatgcca ccatctccag taccgccaac
     3961 acgctgcaaa tcatcacggt ggccgccata ccccaggcga tgcaaaacat ccagaacaat
     4021 gacttcaaag cgcgaaggag aaacctgcaa taagttttcc agaacctcat cagccaccgc
     4081 atcacgaagc tcttttagcg cctgatctaa ccgatcgtcc gggctgctct ttgcaagttc
     4141 ttcatgatcg ggagagtcgg ctttcggatc taaatcgacg gcatccggcc gtgacttaag
     4201 tttgacattc acaaaatcga aggccagatg gttcgtctcc tgctccgtca ttggctgggg
     4261 atgagacgca acccagtcaa aacccgcagg agtcaggcac catttgccac gcgacaaact
     4321 ttgcgacaac ccggcacgtt ttaaacggtc atgcgcccag cctgcacgat ttttataaac
     4381 aagttgtccg ctggtaatga ctttcgctcg ctggctgtca tccagtccta atgcatccgc
     4441 ggcagcctca tgaacatcac gcgcggctgc accttccggt tttgtcgcca gataacgcag
     4501 aacaggttca ataaatttgt cataggttgg aaccgtcata gtacatcctt gcagaatcag
     4561 gtagatgttt ttcggctact ataacactac caaaataggc gaacacgtta gaaatgagtc
     4621 agttgctgtg accgtggtca ttgcccggaa aggtacagaa agctaagatg cgatgttatg
     4681 ggccttaaat atttggacag gcccacacag caatggatta ataacaatga tgaataaatc
     4741 caattttgaa ttcctgaagg gcgtcaacga cttcacttat gccatcgcct gtgcggcgga
     4801 aaataactac ccggatgatc ccaacacgac gctgattaaa atgcgtatgt ttggcgaagc
     4861 cacagcgaaa catcttggtc tgttactcaa catccc
//
LOCUS       DAJSKJ010000219         4892 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000219 DAJSKJ010000000
VERSION     DAJSKJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.223"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..329)
                     /locus_tag="ON345_003581"
     CDS             complement(<1..329)
                     /locus_tag="ON345_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HCR3073579.1"
                     /translation="MKKVLIAALFSCVSFGVFAQQGGFLGSEAERSTTVVQAKEQKDE
                     AWVILEGHIVKKLGDDRYEFRDSSGTIVTDIDESVWAGQNVSPEDKVIIEGELDKDLN
                     SVELDVKT"
     gene            complement(447..602)
                     /locus_tag="ON345_003582"
     CDS             complement(447..602)
                     /locus_tag="ON345_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR3073580.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(848..952)
                     /locus_tag="ON345_003583"
     CDS             complement(848..952)
                     /locus_tag="ON345_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073581.1"
                     /translation="MSDDLSLAMEGALAVISVVGVYCLVVFLMDRMGN"
     gene            complement(1068..1373)
                     /locus_tag="ON345_003584"
     CDS             complement(1068..1373)
                     /locus_tag="ON345_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073582.1"
                     /translation="MNSITKERIELFIKNPLDNGLTRGEQMELARIALALLEAEPVGK
                     LHEYKPVEYQRSVYELTMLVKQLARHLKKAKLDCKLPDKAMDYLKRNGLISVEDVLR"
     gene            complement(1375..1566)
                     /locus_tag="ON345_003585"
     CDS             complement(1375..1566)
                     /locus_tag="ON345_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073583.1"
                     /translation="MNGQISIVRLGACDDREIRMIIRLAMEKTITALITPENLALALT
                     GKSDLPIELKLRNVEIKVK"
     gene            complement(1624..2046)
                     /locus_tag="ON345_003586"
                     /pseudo
     CDS             complement(1624..2046)
                     /locus_tag="ON345_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
     gene            complement(2062..2364)
                     /locus_tag="ON345_003587"
                     /pseudo
     CDS             complement(2062..2364)
                     /locus_tag="ON345_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            2359..2703
                     /locus_tag="ON345_003588"
     CDS             2359..2703
                     /locus_tag="ON345_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR3073584.1"
                     /translation="MTDEIPLDDALLQLREFIDENSGEFFIQVWGNGANFDNTILRRS
                     YERQGIPCLWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSA
                     IWQKLIPSQADF"
     gene            2768..3016
                     /locus_tag="ON345_003589"
     CDS             2768..3016
                     /locus_tag="ON345_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3073585.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            2994..4124
                     /locus_tag="ON345_003590"
     CDS             2994..4124
                     /locus_tag="ON345_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR3073586.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAVTVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPVFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGAAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTQILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(4170..4808)
                     /gene="ompW"
                     /locus_tag="ON345_003591"
     CDS             complement(4170..4808)
                     /gene="ompW"
                     /locus_tag="ON345_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR3073587.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
BASE COUNT         1307 a         1241 c         1060 g         1284 t
ORIGIN      
        1 gtttttacat ccagttcaac actgttcagg tctttatcaa gttcaccctc gattattacc
       61 ttatcttccg gagacacatt ctgcccggcc catacacttt catcgatatc cgtgacaatg
      121 gttccgctac tgtcgcgaaa ctcataacga tcatcaccca attttttaac gatgtgccct
      181 tcaaggataa cccatgcctc gtccttctgt tcttttgcct gcactactgt tgttgaacgc
      241 tctgcttcag accccagaaa acctccctgt tgtgcaaaaa cgccaaaaga cacgcaggaa
      301 aaaagggctg cgattaatac ttttttcatt atcgtcctct tcaagaaatt aacatgaaca
      361 cttgcattgc agcatataaa atgcgggtgc gttgaggatg cctgacacat cagaggtggc
      421 gagggatttc tccctcgcct ggtctcttac ttctcagatt cgtagtctac gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggctcg taagaggcta acctagattt accgttcata
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgt gtaatgttaa ctggggcttt
      721 tctctgtctg ccttacggtg gcatgcccga ggcagacagc ctcaagcacc cgcagcaatt
      781 ctacttaact ctcgctttac cgcaaaccgt ttttacccga tatgggaatc cccatatcgc
      841 aatgaaatca gtttcccatc cgatccatca aaaacacaac caggcagtaa acgcccacaa
      901 cagaaataac ggccagcgcg ccttccattg ccagtgaaag atcatccgac atattccctc
      961 ctttggtgtg aatcccggcg aacgttttta cccccaccga caaataacat atactaaaaa
     1021 agcgatagcc atagcaacgc ctgtaattgc aaaggcttca ggccagttca tcgtaaaaca
     1081 tcctccacac ttatcagtcc atttcgcttc agatagtcca tcgccttatc cggtaatttg
     1141 cagtccaact tcgctttctt cagatgcctg gctaactgct ttaccagcat ggttaactcg
     1201 tagaccgaac gttgatactc cactggtttg tattcatgca atttaccaac tggctctgct
     1261 tccagcaatg ccagtgcaat ccgtgccagt tccatttgtt cgccacgggt aagtccgtta
     1321 tcaagcggat ttttaatgaa taattcgata cgttctttgg taatagaatt catgctattt
     1381 caccttaatc tcaacatttc gcagctttaa ctctattggt aggtctgact ttcctgttaa
     1441 tgctaatgcg agattttcag gagtaatgag agcagttatt gttttctcca tcgccagacg
     1501 aataatcatt cgtatttcgc gatcgtcaca tgctcctagt cgaacaattg atatttgtcc
     1561 gttcatctca ctctcctttg atgcgaatgc cagcggtaat tgaagcctga tagctaattt
     1621 cactcacagt accgcctcct gaaaattacc ctgatagaaa gccagtacac gccgcatagc
     1681 ttcgctcttc cggcactcgc gacagattat gttctgtcgt ctgttgtaac gacgtatttc
     1741 tccgtctggt aattaccaga taagctcagg atcaaccaca accggtttct tcacctttgc
     1801 ccttgatagt tttttgcggg cgttttgcca gtctttacgc gcctgctcag acgggaataa
     1861 tccgtagcct gaattgtaaa catcaccact ggcaaccaat tctctggcga gagtacttat
     1921 gtaatacctt gatgcaccgg ttttagcctc cagagcccgt aacgtctcgc gaccgctcag
     1981 acgtacaagt tcaacaacct gccctttaat tttttcccgc tcttctggtg taaatacttt
     2041 ttccataggt gcctccggca atcacttttc cgacacaata cgactggagg aatcgaaaat
     2101 ctgtcgaaca atatcccggt gcgtgttcag ctcccgcagc gcagcacaga tgcgttccca
     2161 cttctggaca tcacttttcg cccggcgcag ctcgcggtta gccacatgca gcgatggtag
     2221 aatcaggtca tctgctttca tttcgctgat cgatggctgt aacttcacaa tgtcttccac
     2281 gatttctgtt ttcatttcct cctgtgccgt cgtttcctgg actggtaacg caacacctgc
     2341 tggctgagga aaggctttat gaccgatgaa atcccgttag atgatgcact gttacaactg
     2401 cgggaattta tcgacgaaaa ctccggtgaa ttttttattc aggtctgggg aaatggagcc
     2461 aacttcgaca acacgatttt gcgccgttca tacgaacggc aggggatccc ctgcctgtgg
     2521 cgttactaca acgatcgcga tgtacgcaca atcgttgagc tggggaaagc catagacttc
     2581 gatgccagaa cggctattcc attcgaaggt gagcgccata atgcacttga tgacgcccgt
     2641 tatcaggcaa aatacgtttc agccatctgg caaaaactga tcccgagtca ggctgatttt
     2701 taatgttcaa ccccggtcgt cgtctgcaag ctatagtggc ggcggccatg attagcgaac
     2761 aacgctcatg gcaagactta ttctgctcac tgagtgggca aaagaggaat tcagtgaacc
     2821 ggtccctact ccgggcacgt taagtaaata cgctaaagcc ggaatgatat ttcctctccc
     2881 caaaaaagtt ggaagacact ggcgagtgga tcctcgagct cgctttgtcg gaatggtaaa
     2941 caagccggaa gtgatcgcca cagatcaccc tgctttgaag aggatactgg aagatggcgc
     3001 gcccacgaaa atataaaact gaagttccgg gattatctcc gtattttgac aaaagaaata
     3061 acaaagttta ctggcgttac aggcatccca taacaggaaa aaatcacggg ctcggcagta
     3121 ttgaccagaa acaggcagaa gctattgcag cagaagcgaa cagccgtctt gccagacaac
     3181 aaatggaaca aatgctcact ctgcaggaga aaattattcg tgataccggt ggttcatcaa
     3241 ccgtttctgt ttttctgaat agttaccgaa aaattcaaca ggaaagatat gaaaacggag
     3301 agatcaaact caacacactg aaacagaaag catcccctct cagggtattt gatgaacgtt
     3361 ttggtaccag accattagat gccgtaaccg taaaagatgt ggtatcggtg ctggaagatt
     3421 acaaggcaaa aggacataac agaatgggac aaattttcag gaaagtactg atcgatgttt
     3481 tccgggaggc ccagcaaacg ggcgatgtcc cgccggtttt caatcctgca gaatcggcaa
     3541 aaaaaccaca ggtacggata tcaaggcaac ggctgacctt tgatgagtgg atgatgattt
     3601 ataatgcagc ggaaaaggat ggttactttt tacaacgcgg tatgctgctg gcactgatga
     3661 caggccaacg cctttcagat atttgcaaaa tgcaattttc ggatatccgg gatggttatc
     3721 ttcatgtcga acagcaaaaa acaggaaccc ggattgccat ccctctggct ctgcgttgcg
     3781 ataaattaaa tctcaccctg gatgatgtag tgtcatcctg tcgcgattgc gttcttagtc
     3841 cgtggctatt gcaccaccat catgctaaag gggcagctaa gcgtggcggg atggttaagc
     3901 cagcaacgtt aaccgttgca tttaaaaaag cccgagattc ggtagattac aactggcgtg
     3961 ctaatggcac tcccccctct ttccatgagc agagatcatt atcagagcga ttgttcaggg
     4021 aacagggaat tgatacccaa attttgctgg gtcattcgaa tcaaaaaatg accgatattt
     4081 acaacgatgt acgcggaaag gagtggaaaa aactggtcat ttgatgacca gttttgcaga
     4141 ggggttttgc agagattttg cagagaaaat taaaagcggt acccggcaga gaacataaac
     4201 acccacggat ccagtcgtac cgagtctttc acggtagtaa caccagattt atacttagcc
     4261 gtggtatcga tatccatgta ccacactgac atgttaacca accagtcacg gttaatcaga
     4321 taatcaaccc ccacctgccc ggcagctccc caggaatctt tcagactgag atcggaaagc
     4381 cccgcctctt tgccatgatc gttaaatcca ttatcaaaga aggtggtgta gttaatacct
     4441 gccccaacat aaggacggaa tttgctgctg gcatcaccaa aataccactg cgccatcagt
     4501 gttggcggca gatgatgaac ggtcgcaata tcgccggtcg cccgggtgcc gattttatgg
     4561 cggaacggcg tcgctgccag taattccaca ccaatgttgt cggtcgccat ataagtaaac
     4621 gtcaggccca gttgcgtgtt attggtcacg ctgaatccac ccagacttcc taacgtacca
     4681 ccagcacctt ctgttggacg tacggttgca gaacctgcac gcataaaaaa ttcgcctgct
     4741 tcatgcgcaa aggcactgcc agagagaaga gttgttactg ccaaagccgc cactgttaac
     4801 tttttcatat ccgctccgtc gttatggtta taaaaacgtg acgaatatac ctacattgga
     4861 gtaacaaatg atcctacata gatcacatta ca
//
LOCUS       DAJSKJ010000220         4794 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000220 DAJSKJ010000000
VERSION     DAJSKJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.224"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1324
                     /locus_tag="ON345_003592"
     CDS             <1..1324
                     /locus_tag="ON345_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR3073588.1"
                     /translation="LSDNYNAITLGNSWNTPLGAISFDATRSSSKLNNDTRHEGTSYQ
                     VAYNKYLLQTATHFSVAAWRYASQDYRTFSDHLYENDKHNHQSDYDDFYDIGRKNSLS
                     ANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNSWQRISYTFSASQSYDEND
                     KEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLN
                     YGIYVNQQQQNNDTSLGTNLSWKTPFAIIDGSYSHSKNAWQSGGSISSGLVVWPGGIN
                     ITNQLSDTFAILDAPGLEGANINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSES
                     ETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDFQENNIG
                     VVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTIIFQHVIDENKTYICQ"
     gene            1337..2296
                     /locus_tag="ON345_003593"
     CDS             1337..2296
                     /locus_tag="ON345_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR3073589.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGGRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFSLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTYGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2334..3233)
                     /gene="nhaR"
                     /locus_tag="ON345_003594"
     CDS             complement(2334..3233)
                     /gene="nhaR"
                     /locus_tag="ON345_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR3073590.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3299..4465)
                     /gene="nhaA"
                     /locus_tag="ON345_003595"
     CDS             complement(3299..4465)
                     /gene="nhaA"
                     /locus_tag="ON345_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR3073591.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1439 a         1086 c         1010 g         1259 t
ORIGIN      
        1 tttatccgat aactataatg ctataactct gggaaatagt tggaatacac cgctgggtgc
       61 catctctttt gacgctaccc gatcgagtag taagttaaat aatgatacca gacacgaagg
      121 tacgagctat caggtcgcct ataataaata tctattgcaa acggccactc actttagcgt
      181 ggccgcctgg cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa
      241 tgataaacac aatcatcaga gtgattatga tgatttttat gatattggca gaaaaaatag
      301 cctttctgcc aatattatgc aacctttatc caataatctg ggaaatgtat cattaagtgc
      361 actttggcgg aattattggg ggcgaagtgg aaatgctaaa gattaccaat tcagttattc
      421 caatagctgg cagcgcatca gttatacttt ctctgccagc caatcctatg atgaaaacga
      481 taaagaagag gagcgtttta atctgttcat ctccattcct ttctactggg gggatgatat
      541 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
      601 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      661 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      721 gaaaactccc ttcgccataa tagatggcag ctatagccat tctaaaaacg cctggcaaag
      781 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      841 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcga atattaatgg
      901 acaaaaatac aaccgaacaa atagcaaagg ccaggttgtt tacgacctga ttatacctca
      961 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaaccg aattgcaggg
     1021 caatcgtcaa attattgcgc cttaccgtgg agcagtttct tatgtgcagt ttacaactga
     1081 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
     1141 ctatgacgta ctggatttcc aggaaaacaa tattggagtc gttggccagg gtagtcgcct
     1201 ttttattcgc gtagatgaaa ttcccacagg cataaaagtt gctctcaatg atgaacagaa
     1261 cttattctgt actattattt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
     1321 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
     1381 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
     1441 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
     1501 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1561 gaacgcttta aggtttaaag tcggaggtcg tcattatatt attaatttcc cctttgaatc
     1621 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1681 tgcgataaat ttcactattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1741 agatattttt ccaggaactc ctttttcttt gttgttcgaa attgaagtga gttcatgtac
     1801 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1861 ggtaaattta atgacctatg gttttgaaga tcaatccatc gacactggac gatttgtact
     1921 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1981 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     2041 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     2101 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     2161 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     2221 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     2281 ggttatgtac aactaatcta tctcctccac tttaactttg ggggagctgc cgattaacgc
     2341 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     2401 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     2461 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     2521 attgcattgt gcattgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2581 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2641 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacgg tttttctggt
     2701 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2761 tcctgctgcg tagagtctat cggacagtcg gaaatgatca tatccagttt atgctggctt
     2821 aattgctcca gtagcatttc gtgggtggat tcgaagcagc gaagatgaat cggctcgcct
     2881 tctaccactg cagcattcag cacgctactg accaggcgtt tggaaagtgc atcagccacg
     2941 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     3001 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     3061 agaccacgtc ccttgcgctt aaataatttg ccttgcaggc gctcttccag agcccggatc
     3121 tgtccggtaa tggtttgtgg tgtcaaataa agcgcctctg ctgcgccaac cacggaacct
     3181 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     3241 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     3301 acactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     3361 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     3421 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     3481 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3541 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3601 gcagaatgga ggtcaagcca tccagcgtga caccttgcag tgaaacgcca gcattagcaa
     3661 atgcaaacag cggcaaaatc agatacgcca cccacgggtg taacacatgc tccagtcgct
     3721 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3781 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacactacg ccaaccagaa
     3841 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3901 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3961 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     4021 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     4081 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     4141 ccggcacaat catcccacca atagcggcga taactggaaa tgcggcctgg cgtaagctgg
     4201 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     4261 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     4321 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     4381 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     4441 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     4501 actattttac cccgcacgaa tcatcgttaa aatcagatta tagtgtagga ataattcgtt
     4561 ttttacgagc ctttgtagga tttgcgccat cagcggtgag atgagttttt gacggctgta
     4621 agcgctgacg agtgacccta agcccagata atcatagctt aaggtgagga tttctgaggg
     4681 attaattagg aaaagtaata attaatttct tgtcagtcgg cgttatttgt taggacaata
     4741 tttcaaaaat aatccagctt gtcgtcaata attgcgataa agagataaat taaa
//
LOCUS       DAJSKJ010000221         4751 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000221 DAJSKJ010000000
VERSION     DAJSKJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.225"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(49..425)
                     /gene="rnpB"
                     /locus_tag="ON345_003596"
     ncRNA           complement(49..425)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ON345_003596"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(458..1603)
                     /gene="garK"
                     /locus_tag="ON345_003597"
     CDS             complement(458..1603)
                     /gene="garK"
                     /locus_tag="ON345_003597"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR3073592.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1700..2590)
                     /gene="garR"
                     /locus_tag="ON345_003598"
     CDS             complement(1700..2590)
                     /gene="garR"
                     /locus_tag="ON345_003598"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR3073593.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2620..3390)
                     /gene="garL"
                     /locus_tag="ON345_003599"
     CDS             complement(2620..3390)
                     /gene="garL"
                     /locus_tag="ON345_003599"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR3073594.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     IDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3406..4740)
                     /gene="garP"
                     /locus_tag="ON345_003600"
     CDS             complement(3406..4740)
                     /gene="garP"
                     /locus_tag="ON345_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR3073595.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1123 a         1385 c         1152 g         1091 t
ORIGIN      
        1 cgctcctcca aaagcaaaaa cccgccgaag cgggttttta cgtaaatcag gtgaaactga
       61 ccgataagcc gggttctgtc gtggacagtc attcatctag gccagcaatc gctcactggc
      121 tcaagcagcc tacccgggtt cagtacgggc cgtaccttat gaacccctat ttggccttgc
      181 tccgggtgga gtttaccgtg ccacggactg ttaccagccg cgcggtgcgc tcttaccgca
      241 ccctttcacc cttacctgat cccgcttgcg cgggccatcg gcggtttgct ctctgttgca
      301 ctagtcgtgg gtttcccccc caggcgttac ctggcaccct gccctatgga gcccggactt
      361 tcctcccctc cgcccgtctc ccccgaagag gacgacgacg aagcggcgac tgtctggtca
      421 gcttcggcgc gcagtataga gggtttgcgc gcccttgtca ccccgcgttg cgcattccaa
      481 tcgccagtgt cgcggcgata ttacgtgaag cacggcagat attgtcataa gccccgcgga
      541 atgcttcgtc caacgtacct atgctggtca atacgctgaa gaccgcatcg atgccatgct
      601 gatgtacaac gccaacatca tcggtcaggc tacccgcaat gccaatcacc ggtttatggt
      661 acttcttcgc cacgtttgca acgccaatcg gtaccttgcc gtgaatactc tggctgtcaa
      721 tacgcccttc accggtgatc accagcgtac aatcgtgaat atgttcctcc agattcagcg
      781 ccgtagtgac gatttcaata ccacttttca gttccgcacc aagaaacgcc atcagcgccg
      841 cacccatacc gcccgcagct cctgcgccgg ggacatcttt cacatcaaca tgcagcgctt
      901 ttttaatgac atcggcatag tgagagaggt tattgtccag ctcaacaatc atcgcttcac
      961 tggctccctt ttgtgggcca aagatgcgcg atgcgccgtt atcgcccacc agcggattgg
     1021 tgacatcaca agcgacgcga atgacgcaat cttttaagcg cggatcgagg ccggaaatat
     1081 caatatcatt cagagtatta agactaccgc cgccaaaacc aatttcattg ccgttggcgt
     1141 cgcataattt cgcccccagc gcctgtacca tgcctgcgcc gccatcattt gtagcgctac
     1201 cgccaatgcc gataataatg tttgtcgcgc cgctctccag cgcctgcagg attaactcgc
     1261 ctgtgccgcg tgaagtggtc acgagtggat cgcgtttttc cgcaggtacc agctccagcc
     1321 cactggccgc cgccatttca ataaacgcgg ttttgccatc gccggagatc ccccaactgg
     1381 cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg acgttcagaa ccctgggtgg
     1441 ctgcaatcat cgcttccacc gttccttcgc caccgtcggc aaccggaata gaaacgtact
     1501 gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc ctgcgcaacc tcgctggcag
     1561 ataaactttc tttataagag tctggggcga ttacgatttt catacctatg cctgttacca
     1621 catgacgccg gagggcgttt ctcttattcg gcctggattc caggcccgga ttgcaatacg
     1681 ccatccgggc acgacgtcat taacgagtaa cttcgacttt cgccagtttt tcgtagtagc
     1741 acgccagggc gctatgatcc gccgttccta aaccatctgc tcgcagtgcc tgcatcatct
     1801 ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc gtgggaagta tccagcgcat
     1861 ttgccagatc cttaatatgc agatcaatac ggaagcccgg cttgaagttg cggtccatca
     1921 ccatcggcgc tttggcatcc agcacggtac tgcccgccag tccaccgcga attgcctgat
     1981 acaccaggtc cgggttaacg cccgctttgg ttgccagcgt tagcgcttct gacatcgcgg
     2041 caatattcag cgccacaatg acctgatttg ccagtttggt gacgttacct gcaccgattt
     2101 ccccggtatg caccacggaa cccgccatcg ctttcatcaa atcatagtat ttgtcgaaaa
     2161 tagccttgtc gccgcccacc atcactgaca gcgtaccgtc gatggctttc ggttcaccgc
     2221 cgctcaccgg agcatccagc atatcaatgc ctttcgcttt cagcgcttcg ctgatttcac
     2281 ggcttgccag cggtgcgata gaactcatat cgatcaatac cgtacctggc ttcgcgcctt
     2341 caatgatgcc attctcaccc agcgccacct ctttcacatg aggggagttt ggcagcatgg
     2401 ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt agacgctgtt tctgcacctg
     2461 cagcaatcac gtcagcaata gcttctgggt tacggtcagc aaccaccagc gagtaacctg
     2521 ctttcagaag gtttttactc attggtttac ccataatccc caggccaata aaaccaactt
     2581 tcatagtcat atcaatcatc tctcttgttg cggtggtggt tattttttaa aggtatcagc
     2641 cagtttctga gtggcagagc ggaagacgcc gagatcgctg ccgacagcca caaacgtcgc
     2701 gccccattcc agataacgac gcgcatcggc ttcgatcggt gcgaggatac cgctgggttt
     2761 gccgtgcgca ctggcacggt taaaaatgtg ctgaattgct ttttgtacat ccgggtgcga
     2821 ggcattgccg agatggccta atgccgcagc cagatcgctg gggccgacga agatgccgtc
     2881 tacgccttcg gtagcggcaa tggcatcgat gttatctacg ccctgctgac tttctatctg
     2941 gaccagaata gtaatgttct tgttcgactg agcgaaataa tccgccacgg tgccaaacat
     3001 attggcgcgg tgagaaacgg agacgccgcg aatgccttcc ggtgggtaac gggttgatgc
     3061 caccgcctgc tctgcttcct cttttgtttc tacaaaagga atcaggaagt tatagaaacc
     3121 gatatccaga agacgcttaa taattaccgg ctcgttggtc ggcactcgca ctactggcgc
     3181 gctggcgctg cctttcaggg ccattaactg cggaataaac gtggaaatat cgtttggcgc
     3241 atgttcgcca tccagcacca gccagtcaaa cccagccaaa ccaagaactt cagtgctaat
     3301 cgggttagag agtgctgacc agcaaccaat ttgtacctgt ttcgcagcca gtgcggcttt
     3361 gaatttattc gggaaaacat cgttattcat cgcttatacc tttgcttatt tctgcaattc
     3421 catacgttta atgtcgccaa ctacgaagag gtagcagacc atcgccatca gcgctgaaca
     3481 tcccacgaaa accagtgctg cattgaagga gtgcagttca cttaccaggt agccaatcac
     3541 cagtggagtg acaatggagg caacattgcc aaagacgtta aagacgccgc cgcagaggcc
     3601 aacaatctct ttcggcgcgg tgtcagaaat caccggccag cccagagcac caaatccttt
     3661 gccaaaaaaa gccagcgcca tcagcatgac caccagcgtg gtgttgttgg tgtagttaca
     3721 taagatgatg gtggaagcca gcaacattcc cagcacaatc ggtagcttac gtgccagggt
     3781 cagggataaa ccgcgtttga tcagataatc cgagaagaca cctcccagca cgccgcccgc
     3841 aaaaccacac agtgccggaa tcgaggcgac cagacccact ttcagaatcg acatgccttt
     3901 ttcctgcacc agataaatcg ggaaccaggt gaggaagaac caggtgatgg tgttgataaa
     3961 atattgtccg aaaaatacgc ccaacatcat gcggttagag agcaattgct tgatgtaatg
     4021 cagtttggga ccgcttgctg ccgcgctgcc cggctttttg tggtccatat cgaccaccgc
     4081 gccattttca gagataaact tcagctcttc cgcagacata cgtgggtgat ctgtcgggtt
     4141 atgaatcaac ttgatccaca gcgccgtcag cacaaaacca atcaccccca taacggtgaa
     4201 gacgtgctcc cagccccagg cgaaagtcag ccagccaagc agcggcgaaa agagcgccag
     4261 cgagaaatat tgcgccgagt taaagatggc ggaggcagta ccacgttctt tcgtcgggaa
     4321 ccaggcggcg acaatacggg cgttcgccgg gaatgatggc gcttccgaga accccagcat
     4381 aaagcgcata aagaacatgg agatccctgc ccaggccagc gggaacatat caacaaagcc
     4441 ttgcaggaag gtgaacagcg accagaaaaa gaggctgtag gtgtaaactt ttttcgagcc
     4501 aaacttatca agcagccagc cgccgggaat ttgcatcagc aggtaggccc agccaaaagc
     4561 ggagaagatg taacccatcg aaaccgcact taactgcaac tcttttgcca cttcggtacc
     4621 agcaatagac agcgttgcac gatcggcgta gttaacggcg gtaacaataa aaataatcag
     4681 taatattaaa tagcgggtat gcacgccttt cttttttacg tcaacggtgt ccagaatcat
     4741 tttatttacc t
//
LOCUS       DAJSKJ010000222         4741 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000222 DAJSKJ010000000
VERSION     DAJSKJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.226"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            513..2471
                     /gene="acs"
                     /locus_tag="ON345_003601"
     CDS             513..2471
                     /gene="acs"
                     /locus_tag="ON345_003601"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR3073596.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            2671..2985
                     /locus_tag="ON345_003602"
     CDS             2671..2985
                     /locus_tag="ON345_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR3073597.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            2982..4631
                     /gene="actP"
                     /locus_tag="ON345_003603"
     CDS             2982..4631
                     /gene="actP"
                     /locus_tag="ON345_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR3073598.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         1057 a         1244 c         1289 g         1151 t
ORIGIN      
        1 cgcttccaca gttacaggtt ttgcgggagc tgccgtttgt tcagcgtgaa cagaagtgaa
       61 aaagaaaaaa ggaatcaata agctgaagat acggcgtgcg tttattttta tccttgtcat
      121 aggggcttca tccgaattgc gccattgttg caatggcggt ttttattgtt tttcacgaca
      181 gtaaccgcac ctacactgtc atgacattgc tcgcccctat gtgtaacaaa taaccacact
      241 gtaaatgttg tctttaatca attgtaagtg catgtaaaat accactttag agttagtcag
      301 tatcttcctc tttttcaaca gcatgcataa ctgcatgttc ctcaaagaat taatcaactt
      361 ttgttggtga ccttcaaaaa ttaccctgcc gtttatttgc acaattctac ttttgcgtga
      421 tctgtcgccc aaatactaaa caaaactgcc aataccccta tatttaacgc ttatgccaca
      481 tattattaac atcccacaag gagaacaaaa gcatgagcca aattcacaaa cacaccattc
      541 ctgccaacat cgcagaccgt tgcctgataa accctcagca gtacgaggcg atgtatcaac
      601 aatctattaa cgtacctgat accttctggg gcgaacaggg aaaaattctc gactggatca
      661 aaccgtacca gaaggtgaaa aatacctcct ttgcccccgg taatgtgtcc attaaatggt
      721 acgaggacgg cacgctgaat ctggcggcaa actgccttga ccgccatctg caagaaaacg
      781 gcgatcgcac cgccatcatc tgggaaggcg acgacgccag ccagagcaaa catatcagct
      841 ataaagagct gcaccgcgac gtctgccgct tcgccaatac cctgctcgag ctgggcatta
      901 aaaaaggtga tgtggtggcg atttatatgc cgatggtgcc ggaagccgcg gttgcgatgc
      961 tggcctgcgc ccgcattggc gcggtgcatt cggtgatttt cggcggcttc tcgccggaag
     1021 ccgttgccgg gcgcattatt gattccaact cacgactggt gatcacttcc gacgaaggtg
     1081 tgcgtgccgg gcgcagtatt ccgctgaaga aaaacgttga tgacgcgctg aaaaacccga
     1141 acgtcaccag cgtagagcat gtggtggtac tgaagcgtac tggcgggaaa attgactggc
     1201 aggaagggcg cgacctgtgg tggcacgacc tggttgagca agcgagcgat cagcaccagg
     1261 cggaagagat gaacgccgaa gatccgctgt ttattctcta cacctccggt tctaccggta
     1321 agccaaaagg tgtgctgcat actaccggcg gttatctggt gtacgcggcg ctgaccttta
     1381 aatatgtctt tgattatcat ccgggtgata tctactggtg caccgccgat gtgggctggg
     1441 tgaccggaca cagttacttg ctgtacggcc cgctggcctg cggtgcgacc acgctgatgt
     1501 ttgaaggcgt acccaactgg ccgacgcctg cccgtatggc gcaggtggtg gacaagcatc
     1561 aggtcaatat tctctatacc gcacccacgg cgatccgcgc gctgatggcg gaaggcgata
     1621 aagcgattga aggcaccgac cgttcgtcgc tacgcattct cggttccgtg ggcgagccaa
     1681 ttaacccgga agcgtgggag tggtactgga aaaaaatcgg caacgagaaa tgtccggtgg
     1741 tcgatacctg gtggcagacc gaaactggcg gtttcatgat cacgccgctg cctggcgcta
     1801 ccgagctgaa agccggttcg gcaacacgtc cgttcttcgg cgtgcaaccg gcgctggtcg
     1861 ataacgaagg taacccgctg gaaggcgcta ccgaaggcag cctggtgatc accgactcct
     1921 ggccgggtca ggcgcgtacg ctgtttggcg atcacgaacg ttttgaacag acctacttct
     1981 ccaccttcaa aaatatgtat ttcagcggcg acggcgcgcg tcgcgatgaa gatggctatt
     2041 actggatcac cgggcgtgtg gacgacgtgc tgaatgtctc cggtcaccgt ctggggacgg
     2101 cagagattga gtcggcgctg gtggcgcatc cgaagattgc cgaagccgcc gtagtaggta
     2161 ttccgcacaa tattaaaggt caggcgatct acgcctacgt cacgcttaat cacggggagg
     2221 aaccgtcacc agaactgtac gcagaagtcc gcaactgggt gcgtaaagag attggcccgc
     2281 tggcgacgcc agacgtgctg cactggaccg actccctgcc taaaacccgc tccggcaaaa
     2341 ttatgcgccg tattctgcgc aaaattgcgg cgggcgatac cagcaacctg ggcgatacct
     2401 cgacgcttgc cgatcctggc gtagtcgaga agctgcttga agagaagcag gctatcgcga
     2461 tgccatcgta acccacaatt gccggatgcg agtcggtaac ggtttgtagg cctgataaga
     2521 cgcgacagcg tcgcatcagg cattgattgc cggatgcggc gtaaacgcct tatccggcct
     2581 acattcggca agggttaccc gagcgttaac cttctcccat aagggagcgg gaattaaaac
     2641 aatccctaca ttacctctgg agaatctgtg atgaatggca ctatttatca gcggatagaa
     2701 gacaatgcgc atttcaggga gttagtcgaa aaacggcaac ggtttgccac catcctgtcg
     2761 attattatgc tggcagttta tatcggcttt attttactga tcgccttcgc gcccggctgg
     2821 ctgggcacgc cgctgaatcc gaacaccagc gtcacacgcg gtattccaat tggtgttgga
     2881 gtgattgtga tctcctttgt tctcaccggt atctacatct ggcgggcgaa cggcgaattc
     2941 gaccgtctta ataatgaagt cctgcatgag gtacaagcat catgaaaaga gttctgacgg
     3001 cgcttgccgc cacactccct ttcgcagcta acgccgcgga tgctattagc ggggccgtag
     3061 agcgccagcc aacgaactgg caggcgatta ttatgttcct gattttcgtc gtgtttacgc
     3121 tcggcattac ctactgggca tcaaaacgcg tacgttctcg tagcgactac tacaccgcag
     3181 gcggcaatat cactggcttc cagaacgggc tggcgattgc cggggactat atgtccgccg
     3241 cctcattttt ggggatctcc gcgctggtgt ttacctccgg ctatgacggc ttaatttact
     3301 cgctgggctt cctggtgggc tggccgatca ttttgttcct gattgccgaa cgtctgcgta
     3361 acctgggacg ctacaccttt gccgatgtag cctcttaccg cctgaaacaa gggccgattc
     3421 gtattctttc ggcctgtggt tctctggtgg tggtggcgct ttaccttatc gcccagatgg
     3481 taggcgcagg taaactgatc gagctgctgt ttggccttaa ctatcacatt gcggtggtgc
     3541 tggtcggcgt gctgatgatg atgtacgtcc tgttcggcgg catgctggcg accacctggg
     3601 tgcaaattat caaagccgtg ctgttgctgt tcggtgccag ctttatggcc tttatggtga
     3661 tgaaacacgt cggctttagc ttcaacaatc tgttcagtga agcgatggcg gtacacccga
     3721 aaggtgtcga catcatgaag ccgggcgggc tggtgaaaga tccgatctcc gcgctctctc
     3781 tgggtctggg actgatgttt ggtacggcgg gcttgccgca cattctgatg cgcttcttta
     3841 cagtcagcga tgcccgcgaa gcacgtaaga gcgtgttcta cgccaccggg tttatgggct
     3901 acttctatat tctgaccttt attatcggct tcggcgcgat catgctggtt ggtgcgaatc
     3961 cggaatataa agacgcggcg ggccatctga ttggtggtaa caacatggcg gccgttcacc
     4021 tggcgaatgc agtgggcggc aacctgttcc tcggttttat ttcagcggtt gcttttgcca
     4081 ccattcttgc ggtggttgct ggtctgacgc tggcgggcgc atcagcggtt tcgcatgacc
     4141 tgtacgctaa cgtcttcaaa aaaggcgcga ccgaacgtga agaactgcgg gtatcgaaaa
     4201 tcaccgtgct gattttaggt gtgattgcga ttatcctcgg cgtgctgttt gagaatcaga
     4261 acatcgcctt tatggtgggg ctggcgtttg ccatcgcggc gagctgtaac ttcccgatca
     4321 ttctgctttc tatgtactgg tcgaaactga ccacgcgtgg cgcgatgatg ggtggctggc
     4381 tggggctgat taccgcagta gtactgatga tcctcggccc gacgatttgg gtacagatcc
     4441 ttggtcacga aaaagccatc ttcccgtatg aatacccggc gctgttctct atcaccgtgg
     4501 cattcctcgg catctggttc ttctcggcaa ccgataactc agcggaaggg gcgcgcgagc
     4561 gtgaactgtt ccgcgcgcag tttatccgct cccagaccgg ctttggcgtt gagcaaggcc
     4621 gcgcacatta atctctcccc ttccccggtc gcctgaccgg ggaatactct tcctctccag
     4681 catgcatcac cttttcccaa aatattaaac aaataaactc attaaaaaat gagcgatttt
     4741 t
//
LOCUS       DAJSKJ010000223         4694 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000223 DAJSKJ010000000
VERSION     DAJSKJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.227"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            29..730
                     /locus_tag="ON345_003604"
     CDS             29..730
                     /locus_tag="ON345_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR3073599.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPTNENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            755..3355
                     /locus_tag="ON345_003605"
     CDS             755..3355
                     /locus_tag="ON345_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR3073600.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3346..4416
                     /locus_tag="ON345_003606"
     CDS             3346..4416
                     /locus_tag="ON345_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3073601.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4428..>4694
                     /locus_tag="ON345_003607"
     CDS             4428..>4694
                     /locus_tag="ON345_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR3073602.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGA"
BASE COUNT         1277 a         1030 c         1183 g         1204 t
ORIGIN      
        1 tattcatatt ttttaagagt gactatttat gaaaacttgc ataacaaagg gaattgtgac
       61 cgtaagttta acggcaatat tactcagctg ctcatccgca tgggcggcag gtaaaggcgg
      121 gattggactt gcagcaacac gtcttgttta ttcagaaggt gaagagcaaa tttcactggg
      181 tgtacgtaac accagtccgg atgttcctta tcttattcag tcatgggtga tgaccccaga
      241 taataaaaaa tcagcagatt ttattattac accaccgcta tttgtgctga atccgactaa
      301 tgagaatctg ttacgcatta tgtacattgg tgcgccgttg gcgaaagaca gagaaaccct
      361 tttcttcact agcgtacggg cagtcccttc aacaacgaag cgggaagagg gaaataccct
      421 gaagattgcc acacaaagcg tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc
      481 cttaggcgag gctccggcga aactgcgttg cacttcgtca gctgacatgg ttacggtcag
      541 taacccaaca ccttatttca ttaccctgac agacctgaaa ataggtggaa aagtagttaa
      601 aaatcaaatg atttccccct ttgataaata ccaattttct ctgccaaagg gggccaaaaa
      661 tagcagcgta acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc aactcaactg
      721 taaatcgtaa gccgtcttca gttaagagag cgagatgtat agaactcatc gacaacacag
      781 cctgttaagc tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc
      841 agcgttcaat gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc
      901 aatggtggcc gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg
      961 tgtcgatatt gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat
     1021 aacgccagag aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt
     1081 taatactgat gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc
     1141 ggagattatt ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc
     1201 agtaccgcaa atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga
     1261 tgaagggatc aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag
     1321 cagcgcagac agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt
     1381 aggcccatgg agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga
     1441 atggaagaat ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac
     1501 cgtaggtgat gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca
     1561 gctggcgtca gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg
     1621 tggtatcgcc aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca
     1681 aacttatgta tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg
     1741 tgatttgtta gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt
     1801 ttccagcgtg ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa
     1861 atacagaacc aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg
     1921 gggcggaccg tggggaacga catggtatgg tggtggacaa tatgctgaat attaccgtgc
     1981 cgccatgttt ggtctgggat ttaaccttgg cgatttcgga gcaatttcgt tcgatgcgac
     2041 ccaggcgaag agtacgctgg cagaccaaag cgaacataaa ggtcagtcat atcgttttct
     2101 gtatgccaaa acgctcaacc aattgggcac taattttcaa ttgatgggct atcgctattc
     2161 gacgtcgggt ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt
     2221 taatgacggt gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac
     2281 caaacgtggc aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta
     2341 tttaagtggt agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt
     2401 tggctacaac acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc
     2461 ccgtggtcaa cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct
     2521 gttgctcccc agaagtaatg atagctatac caggaaaaaa aattacgcct ggatgacctc
     2581 taacaccagt atcgataacg aagggcacat tacacaaaac ctgggtttaa cggagacact
     2641 actcgatgac ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac
     2701 ggctaatggt agcgccagca tggactacaa aggggcgttt gcagatgccc gagtgggcta
     2761 caactacagc gataacggca gtcaacaaca actgaactac gctctttcag gcagtttagt
     2821 tgcccattca cagggcatta ccctggggca atcgctgggg gaaactaacg tgctgattgc
     2881 agcaccaggc gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg
     2941 cggatatacc gttgtcccct atgccacttc ttatcgggaa aatcgaatcg cacttgatgc
     3001 ggcatcgtta aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa
     3061 aggggcgttg gttctggcgg agttcaatgc ccatgcggga gcaagggtat taatgaaaac
     3121 atcaaagcag ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcgtacagac
     3181 taatagcggc attattgatg atgatggctc gctctatatg tccggtttgc ccgctcaggg
     3241 agcgataact gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca gttaccagct
     3301 taccgaacaa caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt
     3361 ggagaaaaat gcgtgttatt actacgacta ttttttgccg ccgtcttaat gctatggtgc
     3421 gctcaaactg ctgcttatag cgggcagtgt cataccactc aggggaatcc gtatattggc
     3481 gtcaattttg gcgttaaaac cctggaggaa gaagaaaata cggctggagt agttaaagac
     3541 aaattttatc agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac
     3601 aatgtcagaa gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac
     3661 aactggtaca aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt
     3721 tctcctactg cggttccttt cgaaaacgtg ggcacaggag cagatacacg atggcatatt
     3781 tgcgatcccg gcggtcaacg tttaggtggc cagggggcta gcggtaatag cggtagcttt
     3841 tccctgaaaa tattgcagcc gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg
     3901 cgattatttg aatgctacaa catacccgca ggtgattcct gcacgactac aggtacaccg
     3961 gttttagtgt attacctgtc tggtactatc aattcacttg gctcatgttc cgtcaatgcc
     4021 ggagaaacaa tcgaggtcga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg
     4081 cataagcctc ttggggccag aacggcagaa cttgcaattc cagtcaggtg taacacggga
     4141 aacgcggggt tagttaacgt caacctgagt ctgacggcaa ccacagaccc cagctatccc
     4201 caggcgatta agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac
     4261 attatttccc ctgctggtgg aacattaccg ctctctattc ctgatgatgc agacagtatc
     4321 gcgcgaatga atgtctatcc agtcagcacg acaggtgtac caccagaaac cgggcgattt
     4381 gaagccacgg caacggtgag aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa
     4441 cgatatttca gtgcgttatc ttgttcttta gcattcttaa catccatgtc gggatggctg
     4501 ggcctgaaca agttagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg
     4561 ccaccaaaag tgcattacaa aatattcata ttggtgattt taatatcagt gattttcagg
     4621 ctgcgaatac cgtaagcact gctgctgatt tgaatatcga tatcaccggt tgtgccgctg
     4681 gtattactgg cgcg
//
LOCUS       DAJSKJ010000224         4674 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000224 DAJSKJ010000000
VERSION     DAJSKJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.228"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            171..1511
                     /gene="dcuB"
                     /locus_tag="ON345_003608"
     CDS             171..1511
                     /gene="dcuB"
                     /locus_tag="ON345_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR3073603.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISIPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1589..3235
                     /gene="fumB"
                     /locus_tag="ON345_003609"
     CDS             1589..3235
                     /gene="fumB"
                     /locus_tag="ON345_003609"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR3073604.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEALKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3358..3987
                     /locus_tag="ON345_003610"
     CDS             3358..3987
                     /locus_tag="ON345_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR3073605.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGILAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4126..>4674)
                     /locus_tag="ON345_003611"
     CDS             complement(4126..>4674)
                     /locus_tag="ON345_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073606.1"
                     /translation="SLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLN
                     VGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGM
                     IGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKY
                     RKVPPEPVHADIPVGAVSDVKA"
BASE COUNT         1128 a         1248 c         1191 g         1107 t
ORIGIN      
        1 ataaatttgc cgctgcttta atcagccaat attcactgtg aggcatttgc taaagccggt
       61 aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac agaacgtgac
      121 tgtgatctat tcagcaaaaa tttaaataag attatcgcga gggttcacac atgttattta
      181 ctatccaact tatcataata ctgatatgtc tgttttatgg tgccagaaag ggtggtatcg
      241 cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc caccttcagc
      301 caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg gcatcggcga
      361 ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag ctgctgcgcc
      421 gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg accattcttt
      481 gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc gccattaaga
      541 acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag atggggatta
      601 tcgccagtcc ggtatcggtt gcggtcgtgt ctctggttgc gatgctgggt aatgtcacct
      661 ttgatggccg ccatcttgag ttcctcgatc tgctggcaat caccattcca tcgacgttaa
      721 tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg gataaagacg
      781 aagagttcca gaaattcatc tccataccgg aaaaccgtga gtatgtttac ggtgataccg
      841 cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg attttcctcg
      901 gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca tccttcggcg
      961 gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc ggggcgctga
     1021 ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc ttccgttccg
     1081 gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc atgttcggtg
     1141 cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat ccgtgggcct
     1201 atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg gcgctggcgg
     1261 cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg gcttcagcac
     1321 cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca gcgattcagt
     1381 ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagc tttattctgc
     1441 cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc gcgatgtacg
     1501 ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca ttttcgaata
     1561 acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct accaggcacc
     1621 tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt acgttagcgt
     1681 tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga ccctgctggc
     1741 gcagcaagcc tttcacgacg cttcttttat gctccgcccg gcacaccaga aacaggttgc
     1801 ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcgc tgcaattctt
     1861 aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata ccggcaccgc
     1921 gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggc ggcggtgatg aagaagcgct
     1981 gtcgaaaggc gtctataaca cctatatcga agataacctg cgctattcac agaatgcggc
     2041 gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa tcgacctgta
     2101 cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg gctctgccaa
     2161 caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac tgaaaaactt
     2221 cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc atatcgcgtt
     2281 tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaagttag caagcgctca
     2341 ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc gcgatgtcca
     2401 gctggaacag gaactgctgg aagaggccct gaaactcggt cttggcgcgc agtttggcgg
     2461 taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg catcctgccc
     2521 ggtcggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa tcaaccgcga
     2581 aggtatctgg atcgaaaaac tggaacacaa cccaggccag tacattccac aagaactgcg
     2641 ccaggccggt gaaggcgaag cggtgaaagt tgaccttaac cgcccgatga aagagatcct
     2701 cgcccagctt tcgcaatacc cggtatccac tcgtttgtcg ctcaccggca ccattatcgt
     2761 gggccgagat attgcacacg ccaagctgaa agagctgatt gacgccggta aagaacttcc
     2821 gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc ctgccggtta
     2881 tccatcaggt tcacttggcc caaccaccgc aggccgtatg gactcctacg tggatctgct
     2941 gcaatcccac ggcggcagca tgatcatgct ggcgaaaggt aaccgcagtc agcaggttac
     3001 cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc cggcggcggt
     3061 actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc tgggtatgga
     3121 agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg atgacaaagg
     3181 taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta agtaacctct
     3241 tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct cttttgcgat
     3301 tgtaatttca cgcttgctgg tgaatagtca gtattttccc tgatttgcga actcaccatg
     3361 acccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac attaatcctg
     3421 atttataccg gcattttggc ccatgacaaa ctcacctggc tgatggaagt gacaccggtg
     3481 attattgtcg tgccgctact gcttgccacc gccagacgtt atccgttaac gccgctgctc
     3541 tatacgctca ttttccttca cgccatcatc ctgatggtcg gcggacaata cacctacgcg
     3601 aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa tccgtatgac
     3661 aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga aattctcgtt
     3721 cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgttcc tggtctgctg cgtggcgctg
     3781 gcgataagcg cgatgtatga attaatcgag tggtgggcag cgctggcaat gggtcaggga
     3841 gcggatgatt ttctcggcac ccagggcgac cagtgggaca cgcaatccga tatgttctgc
     3901 gcgctgcttg gcgcattaac gacggtgata ttcctcgctc gctttcattg ccgccagcta
     3961 cggcgctttg gcttgataac cggataacaa cttgcgcttt gttgatgccg gatgcggcgt
     4021 gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa gacgtggcct
     4081 gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg ctttcacatc
     4141 actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt tgcgatattt
     4201 atccagcaga tggatctgga tcctgcgcag cgtgtcacca ttgaggcgat agaaacggaa
     4261 gtagagaatc agcgttacca tgaaaaacag agttggtaga gcaatcataa taaactgcat
     4321 acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca tccctaacac
     4381 aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag tctgcacgga
     4441 gtaagcgata ctttcacagc gtacgtgcag tttatattca ccgtaatcaa cggtatctgc
     4501 caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca aaatacccgc
     4561 aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga gaacaccaca
     4621 gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata atga
//
LOCUS       DAJSKJ010000225         4647 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000225 DAJSKJ010000000
VERSION     DAJSKJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.229"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(43..570)
                     /gene="gntK"
                     /locus_tag="ON345_003612"
     CDS             complement(43..570)
                     /gene="gntK"
                     /locus_tag="ON345_003612"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR3073607.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(709..1704)
                     /gene="gntR"
                     /locus_tag="ON345_003613"
     CDS             complement(709..1704)
                     /gene="gntR"
                     /locus_tag="ON345_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR3073608.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(1928..2623)
                     /gene="yhhW"
                     /locus_tag="ON345_003614"
     CDS             complement(1928..2623)
                     /gene="yhhW"
                     /locus_tag="ON345_003614"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR3073609.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSAVRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(2746..3783)
                     /locus_tag="ON345_003615"
     CDS             complement(2746..3783)
                     /locus_tag="ON345_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3073610.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFYYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            4115..4603
                     /gene="yhhY"
                     /locus_tag="ON345_003616"
     CDS             4115..4603
                     /gene="yhhY"
                     /locus_tag="ON345_003616"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3073611.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         1159 a         1204 c         1155 g         1129 t
ORIGIN      
        1 agaccctact gctgttaaaa caagcgttaa tgtagtcact acttatttgc cttttttaat
       61 aacctcaatg gtgcttgcca caacaccttc cagcggttga tcgatatcca ccaccagtac
      121 atcggtttcg tccgcacccg gctcctgcag cgtttcaaac tgcgtcacca acatttgggt
      181 tttaaagaaa tggcctttgc gcgctttcaa gcgactttca atcacatcaa aatcgccttt
      241 caggtagatg aaagagagat tcggattacc ttcacgcagc aagtcgcgat agtgcttttt
      301 cagtgcagaa cagacgatca gcgacacttt attggtgcgc tgcatagcaa acgcggcgtc
      361 gttcagcgcc tgcagccacg gtttgcgatc gtcatcattc agcggttcgc cagacgccat
      421 tttttcgata ttgcgccgtg gatggaggaa atcgccatca agaaacgcgg catgaagttg
      481 atgcgccact tcactggcga ccgcagattt gccgctgccc gatacgccca tcaagacgta
      541 aatgtggtga tcatggttag tcgtgctcaa agtggtgccc ccacaataca agaattaaaa
      601 atgttacggg taactgttat cggtaacatt gtccagccgg acaatatcag aagcaatatc
      661 catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat ttgtaggctt aaatagatcc
      721 gcccggtgac aaggtgaaac ctaaatctaa cattttcggt gtcacagatt cgccacgaat
      781 acgcgccagc aggcgttcag cgccaatact gcccatccgc tcacgcggcg tcagcacgct
      841 cgctaaccgc ggctccatca cttggccaat gtcatgaccg tggaaaccgg caatcgccat
      901 atcgtcagga accttcaatc ccagacgctg acattcaaac gccgcgccga ccgccaggtc
      961 atcgttggta cagaacacgc catccagctg cggatattcc cgccgcgcct ggcgaatcag
     1021 ttcaataccg gaagagtaag aagaagattg ctcaaccatc acgctatacg gcaccagacc
     1081 tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg atagtacgtt cgtcgagacg
     1141 cgcgccgaga taggcaatgt ggcaatgccc gcgagcaata atggcggtgg tcatctggcg
     1201 tgccgcttca aagttatcaa aaccgacggc gatatcgagg cacggcgact ggctgtccat
     1261 gagttccacc accgggatcc ccgccacttc aatcatcttt aaggtgcgcg gcgtgtgggt
     1321 acgttcggtg aggatcagtc catcgatatt ccatgagagc atggattcga ggcgttcttg
     1381 ctccatttcc ggtttataac cgtagtgcgc cagcatggtt tgataaccgt gcgcgtcggt
     1441 gacgctttcg attccgcgta atacttccgc gaaaacctgg ttggtgagag aaggtaacag
     1501 gacgccaatc gcccggctgg tagcgttaga gaggatatcg ggcgcacgat tgggaatata
     1561 gcccagttca tcaagagcgg cggcaatctt gccgcgtaga gcgacggaaa cctgctccgg
     1621 gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct acacggtcag ccacatcctg
     1681 aagtacgggt cttttctttt tcatcgtcct gaaggtacaa aagagataga ttctcttagt
     1741 ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg cttgtttaca attattagat
     1801 atacactaaa tcaattgcat cgcattgtgc taacgacgcg gcaaaacccc ggaagcgtgc
     1861 atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa ggcaggcttc aaaaatgccg
     1921 tggagcttta aaccggcggc agatcgaaca gtaacacttc gctgtcgcta tccgcatgga
     1981 tggaaattgc ctgctcatcc cagattgcca gaccatcgct ggtcgaggct ttcacgccgt
     2041 taatggtgac attgcctttc accacctgga tccagacgcg gcgttcagcg gcaatctgat
     2101 gcaccgactg ctcatctttc agcaacgccc aacgatacag ttccatatcc tgatgcactt
     2161 tcagcgaacc atctcgcgca tccggcgaga gcaccagctg tttgccctgc acggcatcga
     2221 agcgacgctg ttcataacgc ggcgtaatgc cattttcttc aggcatgatc cagatctgat
     2281 acagatgcag acgctcggtg ctgtttgggt tgtactctga gtgacgaata cccgtacccg
     2341 cactcataat ctggaactca cccgccggaa cctgctcttt attgcccatg ctgtcctgat
     2401 gctcaacagt accttccagc acgtaggtca aaatttccat atctttatgc gggtgagtgc
     2461 cgaagccctg ccctgcttca atcacgtcgt cgttaatcac gcgcaccgcg gagaagccca
     2521 taaagttcgg atcgtaatag ttggcaaaag agaaagtatg ccaggagtcc agccagccat
     2581 gatttgcatg accacgttca tttgctttgc gtaagtagat catttgtatt cgccccctga
     2641 atgatttcga tgggcttagt gtggacccga tctgcctggg atgatagagg gtgaaaattg
     2701 acccctctgt tcaaaaaaat tgaactattc gaggggccat caaacttact tagcgagagt
     2761 tactgtggag ggagaggctt gctcaaatcc gcgttcaagg atttccagat tggtaagaac
     2821 ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg gtttgataca acgcatcata
     2881 aacgcgcccg taatcgccca tctccggctt catctcttct ctgaccgtca cgccctcgtc
     2941 attgacatac tccagcacac cgaccgaatc atccgctgcg aatcccggtt cgcccggcat
     3001 aatattagcc ttcaggctgg tttcctgctg gtcgataccg tatttaataa acgaaccttt
     3061 cttaccgtga acgataaatt tcggataatc gattttcacc agatggctgg ttttgacgat
     3121 ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg tcgtcaggat tggctttatt
     3181 acgcaggctg cggatgtcat aagcgacgtg atccgggcga ccgaacagag aaataatctg
     3241 gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc tgcggcagtc caggtttggt
     3301 ttctgccacc gggcggtaat agtaaaaatg gctttccact tcgacaatct ctcccagctt
     3361 gccgctttca atcgcttttt tcgccgtcag gaagcaggaa tcaaagcgac gattctgata
     3421 tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac agctctttcg cctgcgcaag
     3481 tgtcggagtg aacggttttt cgaccagcac atttttcccg gcttccagcg cgcgtttcgc
     3541 gtactcgaaa tggctgtccg cgtgggtgca gacaacaacc agcttaacat cgggatcgtt
     3601 tagtacttcg tcgagatcgc tggtgaaatg gatatgggaa taaatgggag cctgttcttc
     3661 cggcttcgca tggcgacgaa aaatatgcgc gacatgccag ctatccttgc ggttaagtac
     3721 atacggcaga tggtaacggg tggtgctttt gccgaagcca ataaaggcgc agttgatgac
     3781 catgatttcg tcctttttaa ggtggttatt tacaccttag cgcaaagcgg acgtggttcc
     3841 tactggagtg cgcataacga acacaagcac tcccgtggat aaattgagaa cgaaagatca
     3901 aaaaaaagcc agcacccggc tggctaagta atactggaag caatgtgagc aatgtcgtgc
     3961 tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa taataatcat tctcattcgc
     4021 acttgtccaa cactttttgc aaaaaaatgc atttgactcg catttgaaag tcaatgatgt
     4081 tgaaagggac atttacccca aagaggacaa aggaatgagt gagatagtaa tacgccacgc
     4141 agaaacacgg gattacgagg ccatcaggca gattcacgcc cagccggagg tgtattgcaa
     4201 cacactacag gtgcctcatc cttccgatca tatgtggcag gagcgactcg ccgatcgtcc
     4261 cggcatcaag caactcgtcg cctgtattga tggagacgtc gtgggccatc tcaccattga
     4321 cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg tcgactctcg
     4381 ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa tgtgcgacaa
     4441 ctggttgcgg gtagatcgca ttgaactaac cgtgtttgtc gataacgccc cggcaattaa
     4501 ggtctataaa aaatacggct ttgaaattga agggactggt aagaagtacg cattgcgtaa
     4561 tggtgaatat gtcgatgcat attatatggc gcgggtgaag taagatagtg ccctttttct
     4621 gagatggata aagggtgtca ttcaaaa
//
LOCUS       DAJSKJ010000226         4641 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000226 DAJSKJ010000000
VERSION     DAJSKJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.230"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            73..576
                     /gene="yeaK"
                     /locus_tag="ON345_003617"
     CDS             73..576
                     /gene="yeaK"
                     /locus_tag="ON345_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR3073612.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGALPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(577..681)
                     /locus_tag="ON345_003618"
     CDS             complement(577..681)
                     /locus_tag="ON345_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073613.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            851..1297
                     /locus_tag="ON345_003619"
     CDS             851..1297
                     /locus_tag="ON345_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR3073614.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1254..2075)
                     /gene="nimR"
                     /locus_tag="ON345_003620"
     CDS             complement(1254..2075)
                     /gene="nimR"
                     /locus_tag="ON345_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR3073615.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2172..3353
                     /gene="nimT"
                     /locus_tag="ON345_003621"
     CDS             2172..3353
                     /gene="nimT"
                     /locus_tag="ON345_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR3073616.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLYAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAINWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDAKGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            3408..3755
                     /locus_tag="ON345_003622"
     CDS             3408..3755
                     /locus_tag="ON345_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR3073617.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3777..4031)
                     /gene="yoaF"
                     /locus_tag="ON345_003623"
     CDS             complement(3777..4031)
                     /gene="yoaF"
                     /locus_tag="ON345_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR3073618.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4214..>4641
                     /gene="dgcP"
                     /locus_tag="ON345_003624"
     CDS             4214..>4641
                     /gene="dgcP"
                     /locus_tag="ON345_003624"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR3073619.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQV"
BASE COUNT         1077 a         1180 c         1150 g         1234 t
ORIGIN      
        1 aataagcaga acaaaaacag ccgttcatga taaccttctg tggttgtttg cttgtaatct
       61 caggagcgtg aaatgactga aatggctaaa ggaagcgtga cgcatcagcg gttaatcgct
      121 ttattatcac aagaaggtgc taacttccgc gtcgttaccc atgaagcggt aggtaaatgt
      181 gaagcggtgt cagaaattcg tggcaccgct ttaggccagg gagctaaggc actggtatgt
      241 aaagtcaaag gcaatggcgt aaatcaacat gttctggcga tcctcgctgc cgatcaacag
      301 gccgatctga gccaacttgc cagccatata ggcggattac gcgcttcgtt agccagccct
      361 gctgaagtcg atgaactcac aggctgtgtc ttcggcgcgc tccccccttt cagcttccat
      421 ccaaaactca aactggttgc cgaccctcta ctcttcgaac gatttgatga aatcgccttc
      481 aatgcaggga tgctggataa atccgttatt ttgaaaacca cggattattt gcgcattgcg
      541 caaccagaac tgctcaattt ccgccgcact gcgtaactag cctgtccgtt caataagcag
      601 aacggacaaa accagcacaa cagcaatggc aaaaaacgat gacgtgataa tcagtgtctc
      661 gacgaacatt tgatcgttca tagcatgccc ccgggagata gctgttttca cgttattgtt
      721 agcgtgcaca aatggcagtt tgatgacagt tcgcctgatt ttttatgcaa aaaacgtaaa
      781 gattttctac ttccttcctg cagcaagcgt aaagtaagca ggcttattat tttttggcaa
      841 ggaaaccacg atgtttgatg tcactttgct gatcctgctc gggttagctg cgctgggctt
      901 tatcagtcat aacaccactg tcgccgtttc aattctggtg ttgatcattg tccgcgtcac
      961 accgttaagc acctttttcc cgtggattga aaaacagggg ctaagtatcg ggataatcat
     1021 tctgacgatt ggcgttatgg cccccatcgc cagcgggacg ctaccgccct caaccttgat
     1081 tcactcgttt ctgaactgga aatcgctggt ggcaattgca gtaggcgtta ttgtctcctg
     1141 gctgggtggg cgcggcgtga ccttaatggg cagccagccg caactggtcg ccgggctgct
     1201 ggtaggcact gttttaggcg tggcgctgtt tcgcggcgta ccggtcggcc cgcttattgc
     1261 cgcgggtctg gtttcgctga ttgtggggaa acagtagtta atcccgcaat atatcggcct
     1321 ggcgtttgcc ccaggccttt cttaaacata gtaataaagg ccgttgttga atcatacccc
     1381 agcgtatgag caactttctg tacagtatcg cctttcacta atccctgaag tgccataatc
     1441 agctgtaact gctgacgcca ctggcgaaaa ctcaaccccg tctcttttac tatcagtcgc
     1501 gcaagattgc gttcactcat cgcaaaaaag ccagcccatt gccctaatga cccccactcg
     1561 acgggccctt tcgccatcat ctccaccatc gtgcggattt taggatgaga agaaacgggc
     1621 aggtgcagct gttgttgtgg ctgctgaggg agttcatcaa acaggacctg aattaatctg
     1681 cgggtcatcg gctcagcacg ctgagtcgtc gtcctgtttg ccagggttaa aatcaattcc
     1741 cggcataagg gcgatatttt caatgtgcaa catgttgtcg gcattgtcac tgcgctgggt
     1801 tcgataaaca gaaaacagag ttcagcgtta gctgtcactt gattgctatg ttccaccccg
     1861 cccggtatcc aaaccgcata ttgcggcggc accatccaca aagcattttc caccgtacag
     1921 gtaattgcac catgtaatgc tagtatcagc tgtcctttgc ggtgctgatg tacaggactg
     1981 gttaactcat ccgttccggc gtgaatacaa aatgcgaccg cggcttcatg gtgtcggtcg
     2041 ggttcatagc cattgagatt caacctgtgc atcattttgt ccgaatttag cgataatttg
     2101 tcattttagc ttgattcaac ataacaataa aaacggtaag gtacagcctc gtttgtaaca
     2161 atgagaagca tatgacctgt tcaacttcat taagcggcaa aaacaggatt gtccttatcg
     2221 ctggcattct gatgattgcc acaacattac gcgtcacctt taccggcgca gcaccgttac
     2281 tggatacgat tcgttccgct tactcgctga cgacagcgca aaccggctta ttgaccaccc
     2341 tgccattatt ggcctttgcg ctaatctcac ctttggccgc cccggtagcg cgacgttttg
     2401 gtatggaacg tagcctgtat gccgcgttac ttttgatctg tgctggtatc gcaattcgct
     2461 ctctcccttc gccttactta ttatttggtg gtacagcggt cattggcggt gggattgcat
     2521 taggcaatgt cttactgcca ggattaatta aacgcgattt ccctcattcc gtcgccagac
     2581 ttaccggcgc atattccctg acaatgggag ctgcggcggc acttggatcg gctatggtag
     2641 tgccgctggc tttgaacggt tttggctggc aaggcgcgtt gctcatgctg atgtgttttc
     2701 ctctgctggc tcttttttta tggctgccac agtggcgaag ccaacaacat gcaaatttga
     2761 gtacctcgcg cgccttacat actcggggta tctggcgttc gccgcttgcc tggcaggtca
     2821 cattgtttct tgggatcaac tcactggtct attacgtgat tattggctgg cttccggcga
     2881 tcctcatcag tcacggctat agcgaagcac aggcgggttc actgcatggt ttgctgcaac
     2941 tagccacagc agctcccggt ttgctgatcc ctcttttctt acatcatgtg aaagaccagc
     3001 gtggtattgc agcgttcgtt gccttgatgt gcgcagtggg cgctgttggg ctctgcttta
     3061 tgccagcgca cgcgatcaac tggactctgc ttttcggttt tggttccggc gcaacaatga
     3121 tactggggtt gacgttcatt ggtcttcggg caagttctgc gcatcaggcg gcggcactct
     3181 cggggatggc acaatccgtc gggtatttgc tggcagcctg tgggccgccg ctgatgggta
     3241 aaatacacga tgctaagggt gactggtccg taccactgat gggtgttgca atactttcac
     3301 tactgatggc gattttcgga ctttgcgccg ggagagacaa agaaattcgc taatatccag
     3361 tgctatagtg acgtgacaaa tcatgcgtga aagggagaac aaacacgatg aatattcagt
     3421 gcaaacgcgt ttatgatccg gctgaacaga gcgatggtta tcgcgtactg gtcgaccgcc
     3481 tctggccgcg cggtatcaaa aaaaccgatt tagcccttga tgagtgggat aaagaaatca
     3541 cgccgtcaac ggaactgcgc aaagcctttc acggcgaagt cgtcgatttt gcaacctttc
     3601 gcgagcaata tcgcgcagaa ctggcgcaac acgagcaaga aggaaagcgg ctggcggaca
     3661 tcgccaaaaa acagccgctg accctgctct acgcagcaaa aaacaccacg cagaaccatg
     3721 cgctggtgct ggccgactgg ctacgtagct tgtgatttta gtacagcatc cggcggttat
     3781 ttttcaccag ccggatggtc ccgtcgccac aatgcccatt catcaatcgt ttcaccgccc
     3841 ggtaatttgc aattgttgct gaccccttgc gctgtcgaca ctggaatgag cgtcccgccc
     3901 ttctgctggc aatagaccga cgccggattt gccataccaa tctgcggcgg tttaggtgct
     3961 tctggctgag aagaggttga acaaccagcc aggaccagca agcaaggcaa agcaaaactg
     4021 ataattttca tttattgatc tcactcattt atctacgctt agagtatcgc ggtatcgttt
     4081 tgttttgcag cactattttt attacattca ctcaaaacat attacgtctt gtttcatctt
     4141 tgttggtgat gttttatcat gcctgcaaag attaaataat cagcatttac ccgccgtatc
     4201 ctggagttgt ttcgtgtcag atcagattat cgcccgcgtc tcgcaatccc ttgccaaaga
     4261 acagtcactg gaaagtctgg tccgacagct tctggagatg ctggaaatgg tcactgatat
     4321 ggaatcaacc tacctgacca aagtggatgt cgaagcgcgc ctgcagcata ttatgtttgc
     4381 ccgtaacagc cagaaaatgc acatcccgga gaattttacc gtctcgtggg attactcgtt
     4441 atgcaaacgc gccattgatg aaaactgctt tttcagcgat gaagtccctg accgttgggg
     4501 cgactgtatt gcggcacgca atcttggcat caccacattt ctaagcacgc caattcactt
     4561 accggatgga tcattctatg gcacgctttg cgccgccagc agtgagaagc gccagtggag
     4621 tgaacgcgcg gaacaggttt t
//
LOCUS       DAJSKJ010000227         4583 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000227 DAJSKJ010000000
VERSION     DAJSKJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.231"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            226..1050
                     /locus_tag="ON345_003625"
                     /pseudo
     CDS             226..1050
                     /locus_tag="ON345_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            1172..4288
                     /locus_tag="ON345_003626"
     CDS             1172..4288
                     /locus_tag="ON345_003626"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HCR3073620.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEPTGDSYQSESDLERELIQDL
                     LNQGYEFTSVKSPTAMLANVRDQLQRLNGVVFNESEWRRFAEQYLDSPSDSSLDKTRK
                     IHIDYICDFTFDDGRLENIYLIDKKNLLRNKVQVIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSDNSLFKYLQLFVISNGTDTRYFASTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLFNYSVFDSSQTLLVMRPYQ
                     IAATERILWKINSSFKAKNWSKPESGGFIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSDNTAGLRRNLDKDDNKIIVTTIQKLNNLMKA
                     EADLPVYQQQVVFIFDECHRSQFGEAQKNLKKKFRRFYQFGFTGTPIFPENALGAETT
                     TSVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLGAAENQQAFL
                     HPLRIQEITQYILNNFRQKTHRTFPGAKGFNAMLAVSSVEAAKAYYTTFKMLQEEAEK
                     KSGSYKRLRVATIFSFAANEEQSAIGDITDESFDTSAMNSSAKEFLDAAIDDYNACFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERPTIDAITLFGDKNTKNVVLEKSYEEYMQGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIDSEKEKKAFVKLFGEYLRAENILQNYDEF
                     ATLKALQKVDISDPEAVETFKAEHYVDDEKFAELQTIRLPAERKVQDYRSAYNDIRDW
                     QRREKAANDRDTSTTDWDDVVFEIDLLKSQEINLDYILGLIFDHNRQKKGKEALTEEV
                     RRLIRSSLGNRAKEGLIVDFIQQTNLDDMPDKASIIDAFFTYAQREQQREAEALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKIGAFIEK
                     FKGVGGHL"
BASE COUNT         1387 a         1049 c         1028 g         1119 t
ORIGIN      
        1 tccgaaatag ctgaagaagc aggcatctca tgcgcagtca catcacaatt tgatgagcct
       61 gtatacctaa acagtttctc ttttggcgtt agatttaaca ataaaattaa attaaccccc
      121 gagttcttaa aatacctttt cagaacaaac aagatgcgta aagaaataag caaaacggcg
      181 agtggcgtca cacgctttaa catttcaaag gctcgattta aaaagattct aatccccatt
      241 ctctgtccta acaaccctga aaagtccctt gcgatccagg ctgaaatcgt ccgggttctg
      301 gataccttta ctgcccttac cgctgagctt accgctgagc ttaccgctga gcttaacatg
      361 cgcaaaaaac agtacaacta ctatcgcgac cagttgctga gttttgatga gggtgaagtt
      421 gagtggaaga ctttggggga aattggtgag tttattcgtg gaaaacgttt tactaaagca
      481 gattatgtag aagatggtgg tatcagtgta atccattatg gtgagatata tacacgatat
      541 ggtgtttaca caactcattc gttatcacag gttagagctg atatggcagc gtcattacgc
      601 tatgccaaac atggagatgt cgtgataact gacgtggggg agactgtaga agacgttggt
      661 aaagcagtgg catggcttgg ggatgacgat atcgctattc atgatcactg ttatgctttt
      721 cgacattctt tgaatccaaa atttatttcc tattacatgc aaaccgattc gtttatttct
      781 gaaaaagcaa agtatgtagc cagaacaaaa gttaatacac tactcattaa tggtttttca
      841 aaaattatga ttccggttcc atatcctaaa gatcacgaaa aatcacttaa ggaacaagct
      901 cgcattgtcg aaatactcga taaattcgac accctgacca actccatcac tgaaggtctc
      961 ccgcgtgaaa tcgagttgcg ccagaaacaa tacgaatact accgtgattt acttttcagt
     1021 ttcccgaaac ctgaaactgt cagtaattaa ttgaccattg ctaccgatcg ggccacctta
     1081 acacccggtc agtatataga ctatttttta cgcgccggaa gtcaccattc ccccccttcc
     1141 ggcccttgcc agacggcaca aaggatgcgc tatgactcat cagacacaca ccattgctga
     1201 atccaataac tttattgtcc ttgataagta catcaaagct gagccaacag gcgacagcta
     1261 ccagagcgaa tcggaccttg agcgggagct gattcaggac cttcttaatc agggttatga
     1321 gtttacctcc gttaagtcac cgactgcgat gctggcgaat gtgcgggacc agcttcagcg
     1381 tcttaatggt gtggtgttta acgaaagtga atggcggcgc ttcgcagagc aatatctgga
     1441 cagccccagt gacagcagcc tggacaaaac ccgtaaaatc catatcgact atatctgtga
     1501 ttttactttt gatgatggtc gtctggaaaa catctatctg atagataaaa agaacctgct
     1561 gcgcaataag gtgcaggtca ttcagcagtt tgagcagaca ggttcacacg ctaaccgtta
     1621 tgacgtcacc attctggtca acggcctgcc actggtgcaa atcgaactca aaaagcgcgg
     1681 cgtggcgatt cgtgaagcct ttaaccagat acaccgttac agcaaggaaa gctttaacag
     1741 cgataactcc ctgtttaaat acctgcaact cttcgtaatt tccaacggga ctgatacccg
     1801 gtactttgcc agtaccacta agcgggataa aaacagcttt gatttcacca tgaactgggc
     1861 gaaatcggat aacaccctga taaaggacct gaaggacttt accgccactt tcttccagaa
     1921 gcataccctg ctgaatgttc tgtttaatta cagcgtgttt gacagcagcc agacgctgct
     1981 cgtcatgcgc ccctatcaga ttgctgccac tgagcgaatc ctctggaaaa ttaacagctc
     2041 atttaaagcc aagaactggt caaaacccga aagcggtgga tttatctggc ataccacagg
     2101 atcaggaaaa accctgacca gctttaaggc cgcacgactg gctactgagc tggactttat
     2161 cgataaagtc ttcttcgtgg tcgacaggaa agacctcgat taccagacca tgaaggagta
     2221 tcagcgcttc tctccggaca gtgtcaatgg ttcggataat acagcaggcc tcagaagaaa
     2281 cctggataaa gacgataaca aaatcatcgt cacaaccatt cagaaactca ataacctgat
     2341 gaaagccgaa gctgacctgc ccgtctatca gcagcaggtg gtgtttatct ttgatgagtg
     2401 tcaccgcagc cagtttggtg aggcccagaa aaacctcaaa aagaaattca gacgcttcta
     2461 tcagtttggt tttactggta cgcctatttt cccggaaaac gcactgggag cagaaaccac
     2521 aaccagcgtg ttcgggcgtg agttacattc ttacgtcatt actgatgcga ttcgtgatga
     2581 aaaagtgctg aagtttaagg tggactataa cgatgtccgc ccacagttta agtctcttga
     2641 aacggaaact gacgagaaaa aactcggcgc ggctgaaaac cagcaggcat ttttgcaccc
     2701 cctgcgtatt caggaaataa cgcaatacat tctcaataac ttccgtcaga aaacgcaccg
     2761 aaccttcccg ggagctaagg gctttaacgc catgttagca gttagcagcg tggaagcggc
     2821 aaaagcttat tacacgacgt ttaaaatgct acaggaagaa gcggagaaga aatctggcag
     2881 ctataaacgc cttcgggttg caaccatctt ctcctttgct gcaaatgaag agcaaagcgc
     2941 cattggtgat attactgacg aaagctttga taccagcgcg atgaacagta gtgcgaagga
     3001 atttctggat gcagccattg atgactataa cgcctgcttt aaaaccaatt tcagcactga
     3061 cagtaacggc ttccagaact actaccgcga tttagctcaa cgagtgaaaa atcaggatat
     3121 cgaccttctc atcgtggtgg gaatgttctt aaccggcttt gatgctccga cactcaatac
     3181 tctgttcgtt gacaaaaacc tgcgttatca cggtctgatg caggcattct cacgcaccaa
     3241 ccgtatctat gatgcaacca aaaccttcgg caatatcgtg actttcaggg acctggagcg
     3301 tccgaccatt gatgccatca cactttttgg cgacaaaaac accaagaatg tggtgctgga
     3361 aaaaagctac gaagagtaca tgcagggctt taccgatgcg gcaaccggtg aggctaaacg
     3421 aggttttatg gctgttgttt cggagctgga gcagcgcttc cctgaccctg ccagtatcga
     3481 cagtgaaaaa gagaaaaaag ccttcgtcaa actgtttggt gaatatctgc gggcagaaaa
     3541 catcctgcaa aactatgatg aatttgccac gctgaaagcc ctgcagaagg ttgatattag
     3601 tgacccggaa gcggtagaaa cctttaaagc agagcactat gtggatgatg aaaaatttgc
     3661 tgaattacaa accattcgcc tccctgccga acgtaaagtt caggactatc gctcagcata
     3721 caatgatatc cgggactggc agcgacgcga aaaagcagca aacgacaggg atacatcaac
     3781 cacagactgg gatgacgtgg tgtttgaaat tgacctgctt aagtcgcagg agattaacct
     3841 ggactacatc cttggtctga ttttcgacca caatcgtcag aaaaaaggta aagaagccct
     3901 gactgaagag gtcagacgct taatccgctc aagtctgggc aaccgtgcca aagaaggtct
     3961 gattgttgac tttattcagc aaactaacct tgatgatatg ccggacaagg ccagtatcat
     4021 tgatgcattc tttacctatg ctcagcgtga acagcaacgg gaagcagagg cgttgattaa
     4081 ggaagaaaac ctcaatgaag aggctgcaag gcgctatatc cgcacatcac taaaacggga
     4141 gtatgccacc gaaaatggga cggagctaaa tgagacgcta ccaaagctca gtccgctgaa
     4201 tcctcagtac aaaacgaaaa agcagaccgt attccagaaa attggggcat ttatcgaaaa
     4261 atttaaaggg gttggaggac acctctagat gtcagataaa agaacaatgc gctaccgtaa
     4321 tttataggta agattgtatc ttttcacctg catcagacct gttatgatcg atgaggcgct
     4381 ggttaatgtt aatgctaact ggatgagttt taaacgctga ttcagacaat tagcgtccag
     4441 aacttacaga aaccagtgct tgattttgga tggtaaagtg ctgaacggac gtatcacagg
     4501 agaatgctta tgagcgtaac tataaaagac gatcgactcc gtaccatcgc tacctttgac
     4561 ggtaaaaccc tgaaagacga ccg
//
LOCUS       DAJSKJ010000228         4577 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000228 DAJSKJ010000000
VERSION     DAJSKJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.232"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..191
                     /locus_tag="ON345_003627"
     CDS             <1..191
                     /locus_tag="ON345_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3073621.1"
                     /translation="VTLLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTN
                     KVKSGALEATALINVKYD"
     gene            complement(273..611)
                     /gene="rcnB"
                     /locus_tag="ON345_003628"
     CDS             complement(273..611)
                     /gene="rcnB"
                     /locus_tag="ON345_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR3073622.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(830..1654)
                     /gene="rcnA"
                     /locus_tag="ON345_003629"
     CDS             complement(830..1654)
                     /gene="rcnA"
                     /locus_tag="ON345_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR3073623.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1775..2047
                     /gene="rcnR"
                     /locus_tag="ON345_003630"
     CDS             1775..2047
                     /gene="rcnR"
                     /locus_tag="ON345_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR3073624.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      2139..2235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2270..3058
                     /gene="thiM"
                     /locus_tag="ON345_003631"
     CDS             2270..3058
                     /gene="thiM"
                     /locus_tag="ON345_003631"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR3073625.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSICAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            3055..3855
                     /gene="thiD"
                     /locus_tag="ON345_003632"
     CDS             3055..3855
                     /gene="thiD"
                     /locus_tag="ON345_003632"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR3073626.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            3920..4510
                     /locus_tag="ON345_003633"
                     /pseudo
     CDS             3920..4510
                     /locus_tag="ON345_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
BASE COUNT         1184 a         1120 c         1153 g         1120 t
ORIGIN      
        1 cagtcacact tttaaatatc actgataaca gcaaaggata cacaaattta aaagaaattg
       61 cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc agttactacg
      121 tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta atcaacgtga
      181 aatacgacta ataacaagca agacgagcaa gtggctaata ataaaaaata acaaggtgtc
      241 tgcatttccc cctcatgatg agggggattt ttttagcgat gataaaaaat ctcaccgtcg
      301 taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag
      361 gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg cttaatgttg
      421 tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg cttaaagtca
      481 gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga acccgcggtg
      541 gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa catcttattt
      601 ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata tctttcctgc
      661 catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg taaaaagtgt
      721 aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga aatagctgat
      781 ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt tatcgcatta
      841 tgcccatgaa gccatgtacg cccatataca caccaactaa gccaatcaac agactggaaa
      901 aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg acctgctgaa
      961 cgctgattgc tgcgccaacg cctacggtga ccagcgttaa cgccaggcca atgctgaaac
     1021 tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcaaatc aacagcacgg
     1081 taattgctgc cggacagggg ataagaccac ctgttaagcc aaataacaaa atttgccagt
     1141 tggtgacctc tctaccatca aagcgtcgtt taatatcatt ggcatgggct cgtgcatggg
     1201 catcctgata ctcgccatgt tcgtgatggt gatgatgtcc atggtcgtgg tgatcttcgt
     1261 gatcgtgatg atgatgctca taatcatgcc cgtgcatatt ctccagccag ttgcgttcgc
     1321 cgcgccaggt acgccagaac atccagaacg cggtgctaat aatgatcact gcggaaatca
     1381 gctggagcca cggttctgct gattgagcag taaagcgctt actgatcacc atcccgccaa
     1441 aggcaatcaa ccagaccacc gcggtatgcg aaatggttgc tgccagtccg agcatcaccg
     1501 cctgtttaat ggtgcctttg atggcgatga taaacgccgc catcatcgtt tttgagtgcc
     1561 ctggttccag gccatgaagc gcaccaagta agatggcgct ggggatgaag aaccaggcgt
     1621 ttccttgctg aagaagagtt gtaaattcgg tcatgataat aattcttagt attaattcgg
     1681 caatctgatt ctactccccc ccagtacctg atactacccc ccagtagatt aatagtgcta
     1741 tgatttttca tattcttgtt aaccaggtgt tgccatgtct catacaatcc gtgataaaca
     1801 gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc gtggcgctca agaaaatgct
     1861 cgacgagccg cacgaatgcg ctgcagtttt acaacagatt gctgctatcc gtggcgcggt
     1921 aaacggtctg atgcgggaag tgattaaagg tcatctgacg gaacacatcg ttcaccaggg
     1981 ggatgagcta aaacgtgaag aagatctgga tgtcgttctg aaggtgctgg attcatatat
     2041 caaataattt attaacgcga ttgtaaaact gccgtttttc ctcgtttaca acgcgtgcgc
     2101 tggacattac catcctcctc tgcgatttat catcgcaacc aaacgactcg gggtgccctt
     2161 ctgcgtgaag actgagaaat acccgtatta cctgatctgg ataatgccag cgtagggaag
     2221 tcacggacca ccaggtcatt gcttcttcac gttatggcag gagcaaacta tgcaagtcga
     2281 tctgctgagt tcagcgcaat ctgcgcacgc gttacacctt tttcaccaac attcccctct
     2341 tgtgcactgc atgaccaacg atgtggtgca aacctttact gccaatacct tgctggcgct
     2401 cggtgcatcg ccagcgatgg ttatcgaaac cgaagaggcc agtcagtttg cggctattgc
     2461 tagtgcgttg ctgattaacg ttggcacgct gacgcagcta cgcgcacagt cgatctgtgc
     2521 cgccgttgag caagcaaaaa gctctcaaac accctggacg cttgatccag tagcggttgg
     2581 tgcactcgat tatcgccgcc gtttttgtct ggaacttctg tctcataagc caaccgccat
     2641 acgtggtaat gcttcggaaa tcatggcatt agctggcatt gctaatggtg gacggggagt
     2701 ggataccact gacgccgcag ctaacgcgat acccgctgca caaacactgg cacgggaaac
     2761 tggcgcaatc gtcgtggtca ctggcgagat ggattatgtt accgatggac atcgtatcat
     2821 tggtattcac ggtggcgatc cgttaatgac caaagtggta ggaactggct gtgcattatc
     2881 ggcggttgtc gctgcctgct gtgcgttacc aggcgatacg ctggaaaatg tcgcatctgc
     2941 ctgtcactgg atgaaacaag ccggagaacg cgcagtcgcc agaagcgagg ggccaggcag
     3001 ttttgttcca catttccttg atgcgctctg gcaattgacg caggaggtgc aggcatgaaa
     3061 cgaattaacg ctctgacgat tgccggtact gatccgagtg gtggtgcggg gattcaggcc
     3121 gatcttaaaa ccttctcggc acttggcgct tatggttgct cagttattac tgcactggtg
     3181 gcgcaaaata cccgtggcgt acagtcggtg tatcgcattg agcctgattt tgtcgccgcc
     3241 cagctcgatt cggtgttcag cgatgtgcga atcgacacca ctaaaatcgg tatgttggcg
     3301 gaaaccgata ttgttgaagc ggtggcagaa cggttgcaac gttatcagat ccaaaacgtg
     3361 gtactcgaca ccgttatgct ggcaaaaagc ggcgacccgc tgctttcacc ttcggcggtt
     3421 gctacgctgc gcagtcgatt attgccacag gtttcattaa taacgccaaa cttgcccgaa
     3481 gctgccgcct tgctcgacgc gccacacgcg cgcaccgaac aggaaatgct ggaacaaggg
     3541 cgatcgctgt tggcgatggg ctgtggcgca gtgctaatga aaggtggtca tctggatgat
     3601 gagcaaagcc cggactggct gtttacccgc gagggtgaac aacggtttac cgcaccgcgc
     3661 attatgacca aaaacaccca cggcactggt tgtacgctct ctgcggcgtt ggctgcacta
     3721 cgcccgcgcc atacaaactg ggctgacacc gtacaggagg caaaaagctg gctttcatca
     3781 gcattagccc aggccgacac gctggaagtt ggtcacggta ttggtccggt tcaccacttc
     3841 cacgcctggt ggtgagcacc gtactatagc tgctttggtt attgtcagcc tttggcactg
     3901 acattcaggg atgaaaccaa tgcagctaag aattaccagt cgaaaaaaac tcaccgctct
     3961 attatgcgct cttgggctaa tttccatcgt tgcaatttat ccgcgtcaga cggtgaattt
     4021 tttctattcg acagcagttc agattacaga ctacatccac ttctacggtt atcgcccggt
     4081 taaatctttc gctattcgta ttcctgccag ttacaccatt cacggaatag atgtttcgcg
     4141 ctggcaggag cggatcgact ggcagcgtgt ggcaaaaatg cgcgataacg gtattcgctt
     4201 acaatttgcc tttattaagg cgacggaagg cgaaaagctg gtggacccct atttttcgcg
     4261 taactggcaa ctaagccgcg aaaatggcct gctgcgcggg gcatatcatt atttctcccc
     4321 gtcggtagca gcaccggttc aggtgcgatt atttctgcaa acggtagatt tctcacaagg
     4381 cgatttccct gcggtactgg acgtagaaga gcgaggaaaa ttatcggcaa aagaattacg
     4441 caagagggta agtcagtggc taaaaatggt cgaaaaaagt acggggaaaa agccgattat
     4501 ttactcagga gggctttgtt gaataaataa gatttcgtgc gaacgaccct gtagctggct
     4561 ggattttcag gcaatac
//
LOCUS       DAJSKJ010000229         4566 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000229 DAJSKJ010000000
VERSION     DAJSKJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.233"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(1..332)
                     /locus_tag="ON345_003634"
                     /pseudo
     CDS             complement(1..332)
                     /locus_tag="ON345_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            743..2143
                     /locus_tag="ON345_003635"
     CDS             743..2143
                     /locus_tag="ON345_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR3073627.1"
                     /translation="MAISDAGMFTVKEINRLIILQDVIDRNLRPGKAAEMLGITPRHC
                     SRLLKRYRQSGPLGMNNQSRGRTGNRLLPRSLTDQALGIIRERYRDFGPTLAREKLEE
                     VHGLVLGKETIRRLMIKAGLWIPRKQRPPKIHQPRYRRPCIGELIQIDGCDHHWFENR
                     GRPCTALVYVDDATSRLMHLLFVKSESTFTYFEATRGYIEKYGKPMILYSDKASVFRV
                     NNKHATTGPGETQFARAMRGLNITPLCAETSQAKGRVERAHLTLQDRLVKELRLKGIC
                     TIEAANAFAEEYMADYNRRFAKEPLHDFNAHRPLALDDDLNAEFTWREPRRVSKSLTV
                     QYDKMLYLIEDTEYSRRAIGKYIDVWHYPDGHKELRLHGVLLPYSTYDRLSEVDPVAI
                     VDNKRLGHVLDVARQVQRKRDNNRSQSLPCSGDEPSRRRHAPSINKSQRSLNEDDLLE
                     AMIKLQGSSEEIFGKR"
     gene            complement(2287..3165)
                     /locus_tag="ON345_003636"
                     /pseudo
     CDS             complement(2287..3165)
                     /locus_tag="ON345_003636"
                     /inference="COORDINATES: protein motif:HMM:NF017206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
     gene            3213..4040
                     /locus_tag="ON345_003637"
     CDS             3213..4040
                     /locus_tag="ON345_003637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073628.1"
                     /translation="MGDFVYSAMPDAGHGLSWDWQSNSDIVWADGVTQTASGMSLRTG
                     FARINVMGVKSTVLEKRKKELGWEVSLGTSMPAKFGPEYISMAPSKCGGYLNENCTFN
                     PASSLTKRGIEFHKICSTIAGGPNTTDVYIVRVAKKESMLMAWVNSAGSSASSSWLEL
                     RMYSPESAALYCDETVKKEYSFATQDGRSKKELVHDTKIITDALSKAISGIDGANDAV
                     CHARIFADGYGGISVFDASQKDNDIVFCESIINRLSGIRLPSPSKNASTIINLDVKN"
BASE COUNT         1264 a         1100 c         1073 g         1129 t
ORIGIN      
        1 gtggccccat caagtattaa tacctcaccc gtacggtcca aaatttgaat accgacattt
       61 tgggcagagc ctgccgctga actctgcagc gctaacgtat cgtcattact ggtgacagta
      121 gtacccagga atgcaacagc tgcattactg gaaacattgg tatcacaatc attcagcttg
      181 atattgaagc caacggcgga gctgaggtca cccgctttag ccaggcgaga agaacgaacc
      241 tgacccagct caaccgtttg gttcattgat tctgaatcaa ttgcacaagc ggcatttacc
      301 acttcacctt taaaatgaat aacaccgcca ttatcggtcg cctgacttca ttgagacgta
      361 gccatttctg ataccggagt ggactcattc ccgcttgatc cgtccttaaa taagtaacag
      421 caccctctgg tgttttcgta tgcagtgtct atgcgacaga cactgcgctc ttagcatggg
      481 gcagggaata accaggctat ttctccatgc acaggccaat tatgaaagag gagaggctta
      541 agttcttgaa ccctgaacga ataacgacta ccagaaaagc acttcgcatc actcagacat
      601 gatttaacat aatgtacatt atgcgcacca acattgtaat ggctcaatga taatgtctaa
      661 gtatagctca ttaggaatgt ccaggttata gtttcaatcc tatgcctaga ctcgactcgc
      721 ttatcctgat gaggagttct ctatggctat atctgacgcc gggatgttca ccgtgaaaga
      781 aatcaaccgc ctcataatcc ttcaggatgt catcgaccgc aacctgcggc ctggcaaagc
      841 agcggaaatg ctcggcatca cgccgcgtca ttgcagccgg ttgctcaaac gttaccgcca
      901 gtctgggccg cttggcatga ataaccagag tcgtggccgt accggaaacc gcctgttacc
      961 gcgttcactt accgatcagg cattgggcat catccgggaa cgctatcgcg attttggacc
     1021 gacattagca cgggaaaagc tcgaggaagt tcacggtctc gttctcggta aggaaaccat
     1081 cagacgcctc atgataaaag ccggactttg gatcccccgt aaacaacgcc cgccgaaaat
     1141 ccatcagccc cgatatcgtc gcccctgtat tggtgaactg atacagattg atggctgcga
     1201 ccaccattgg tttgaaaacc ggggtcggcc ttgtactgct ctggtctatg ttgacgatgc
     1261 caccagtcga ctgatgcatc tgctgtttgt taaatctgaa tccacattta cctattttga
     1321 agccacccgt ggctatatcg aaaaatacgg taagcctatg atcctctaca gcgataaagc
     1381 cagtgtcttc agggttaata ataagcatgc caccaccggg cccggtgaaa ctcagtttgc
     1441 cagggccatg cgcggcctca atatcacccc gctgtgcgca gaaaccagtc aggcaaaagg
     1501 tcgtgtggaa cgtgctcacc tgacacttca ggaccggctg gttaaggagc tcaggcttaa
     1561 ggggatctgt acgatagaag ccgccaatgc atttgcagaa gaatacatgg cggattataa
     1621 ccggcgcttt gccaaagaac ccttgcatga ttttaacgcg caccggccgc tggcattaga
     1681 tgatgacctt aatgcggaat ttacctggcg agagccccgt cgggtatcaa aaagtctgac
     1741 cgtacaatac gataaaatgc tctatctgat tgaggacaca gaatacagtc gaagagccat
     1801 cggcaagtat attgatgtct ggcattatcc tgacgggcat aaggaactgc gactacatgg
     1861 tgtgctactg ccctactcca cctatgaccg tctttctgaa gttgatcccg tcgcaattgt
     1921 ggataacaaa agacttggcc atgtgctgga tgttgcccgt caggtccaga gaaaacggga
     1981 caataatcgc tcacaatcat taccttgttc tggtgatgag ccttcccgca gacggcacgc
     2041 ccccagtatc aacaaatcgc agcgttctct gaatgaggat gatctgctcg aagcaatgat
     2101 aaagcttcag ggtagctctg aggaaatttt tggtaaaaga taaacccgag ggacggcgtg
     2161 acggctctta agggccgcta tgagcgaaaa gcggaagtta ctcctgaact ccaccgacat
     2221 ctcaaagcct gtttatttgg actctgagat gccggtcaga atagtaggga tacagtgcga
     2281 tattaatcag atggataatt cgccaattac gaaattatct ttattctgct caatgaactg
     2341 gtcaagggtg gtcagaggca cgccccagct gcgcacagca ttaagatcaa ctttataccc
     2401 ctcgacattg ttccatacct ggcttctgac atgcagcgga tgatccccac gtgcaagcat
     2461 ctcatcctca gatgtggtga cagctgacac aggcttcccg gtagcctctg cgatcttcgc
     2521 tgcaagctcc tctatagtga cggattgtgc ggcgaggtcg atttcatgac cattaaagcg
     2581 tgcagggtca ttaaaggcct cagcggcaaa cgttccgata tcctttgctg cgatccagtc
     2641 cagaggggta tcaagttcaa tagctgtatc gaaaacgccg cgttcggaaa gagtcggaaa
     2701 catgaaatca accataggag gaatgatgtc ctccataatc aaagcaggtt tcagaatcac
     2761 ccagttggga aaaccttgct tcctgaccat ctcatttaca gccgctttgt tgttccagta
     2821 cagcggctcc cagcgcccct ctttccatcc gacaaagctc tcttgatcgc ctgcacgagc
     2881 gacagaggta tgaacgatta cacgtacatt tgcccggtga gcagcctcga ttaatgcctt
     2941 accggtcaca atctcagtct ccgggtcagc gggctgagaa cccatttgca cagagaaaat
     3001 gccgtccacg cctgccagtg ctgcatcgag gctggaaggg tcgttaaagt cgcccttaaa
     3061 gacctccacg ccctgcgctt tcaaagactg tgcagcagct gaattcggat ctcgcgtcag
     3121 tgcacgcacg gagcgcccag cctgcaacag cgcacgcacg acagccccac cttatttccc
     3181 ccgaatctct ttcgggaaag cccagttctg agttgggtga ttttgtttat tcagcaatgc
     3241 cggatgctgg tcacggcttg tcgtgggact ggcagtcaaa ctcagatatt gtttgggctg
     3301 acggtgttac gcaaacagct tccggaatgt cactccgcac tggatttgct cgcatcaatg
     3361 ttatgggcgt taaatcaaca gtgctggaga aaaggaaaaa agaattaggc tgggaggttt
     3421 cccttggaac atcaatgcca gccaagttcg gccctgaata tatatcaatg gcaccatcaa
     3481 aatgtggtgg atatctgaat gagaattgta cattcaatcc tgcctcatca ctcacgaaga
     3541 gaggcattga gtttcataaa atctgctcaa ccatagcggg tggtccgaat actacggatg
     3601 tgtacatcgt cagggtagcc aaaaaagaaa gcatgttaat ggcatgggtg aatagcgccg
     3661 gttcgagtgc aagctcaagc tggctggaac tgagaatgta ctcacctgaa agtgcagcat
     3721 tatattgtga tgaaactgta aagaaagaat actcattcgc cactcaggat ggacgaagca
     3781 agaaggaatt agtgcatgac actaaaataa ttacggatgc attatcaaaa gccatttccg
     3841 gaatcgatgg tgctaatgat gcggtgtgtc atgccaggat attcgcagac ggttatggtg
     3901 gcatatctgt atttgatgca agtcagaaag ataatgacat tgtattctgc gaatccatca
     3961 ttaacaggtt gagtggcatt cggctcccct caccttcgaa aaatgcatca acaataatta
     4021 atcttgacgt gaaaaactaa taaggaaagc aagaaaggaa atgaattctt catttttaac
     4081 aaaatacctg tcaaagttga cgctcaccat taacataatt ctgaccgtaa tgacatggtt
     4141 ctatctaatc ggaactgaca acccgatatg gtattcgata attcagagtt atgttttcat
     4201 cagtttcgat acggcgaaaa tcatcatcac tgttatctgt attgtctgga tagtcagaag
     4261 cattacggac tatgaactga aaacggtgtt atttactcag gctgatattc aaaaggaaat
     4321 tcttaaagag ttgcgagcat taagaaacag caaggaaatg catatgcgga tacagcggga
     4381 gataacttca ggatagactt tgacaaagta gattactgat agctatctga tgagtcctca
     4441 cttctgtggg gattcttatt tttctgttct tattattgtc agctcaagcc gcatggctct
     4501 cctgcggagg gcttaatatt gggataatat tgaaacatca aagacagatg cagataagta
     4561 cagaaa
//
LOCUS       DAJSKJ010000230         4554 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000230 DAJSKJ010000000
VERSION     DAJSKJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.234"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(150..593)
                     /locus_tag="ON345_003638"
     CDS             complement(150..593)
                     /locus_tag="ON345_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073629.1"
                     /translation="MAKIYEFPQGAERSKLKKEIIRERKKRLRETNGNPVIRHAKWFW
                     FYLRLATAGALHLVSVISLAVLGAFSKAIFWIGGMLCVVTWFHLERQFWTPQNFTIPV
                     IVTLWGLSLFATPLMELLNKKMPWYRLLVPDAKHTSTEATNDDQP"
     gene            complement(602..1615)
                     /locus_tag="ON345_003639"
     CDS             complement(602..1615)
                     /locus_tag="ON345_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3073630.1"
                     /translation="MRLLLSLDMKILRSSYLKVRGFLIKGCIVVLNYNLCGVMICLHR
                     KGYMRLIVKTVTGLTKVRHRNEVGVTLASLSLSAKRVLFLALCQIDTKEMLDDDILEV
                     DADFFSKATSLDKYASYAALKEGAKVLSSTTLVLNRDDLKNLADELGILSSKNKIPDR
                     LDLNLTEFCAYYDHLATVRIKFTNTAKRYFSKLIGSENRYTTQVLKSVVLLNSVNSTN
                     LYQVIRKYYSQNSSKKSFDISVDDLKEEMGLYTIEEGEKKYKYPKYSFFVRDVINKSI
                     NEIIEKTEINQLSFSVVGKKGRMAHMLRFEFSINEKSSSFSEDDMAFLEEFDKVVPPK
                     KNK"
     gene            2226..2378
                     /locus_tag="ON345_003640"
                     /pseudo
     CDS             2226..2378
                     /locus_tag="ON345_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            2968..3483
                     /locus_tag="ON345_003641"
     CDS             2968..3483
                     /locus_tag="ON345_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HCR3073631.1"
                     /translation="MNIQEALNVFGLSGDLTEKDIKAAYKKAALKYHPDRNPLGAELM
                     KAVNAAFDFLMANIDKINQFQSTDENARYNYGEDLEKVLNTLSGLTGIVYEVIGNWVW
                     ISGETKEHKDILKEMGCKWASKKKQWFYRPEEHKSRWNRKEHSIEEIREMYGTAGKRK
                     ASGWTRVEASA"
     gene            3542..3778
                     /locus_tag="ON345_003642"
     CDS             3542..3778
                     /locus_tag="ON345_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073632.1"
                     /translation="MKNKTITEAELINIFESYGAYICPDEIEVTAKECNENGSVLHRG
                     LNAEGWAHLFAKEEAYQQECEAQEAASDDGHFDE"
     gene            3838..4389
                     /locus_tag="ON345_003643"
     CDS             3838..4389
                     /locus_tag="ON345_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073633.1"
                     /translation="MKEQKNFFERYKPVFEIVCRILGNGWRVNLLDDCQYRIKLTSPD
                     FKNYSIHIRMEKGRLVIIGSVDSRSWRSPYHTCTVSPERNPVEIAADIEKKILSDALD
                     NVDMAREYEQQLQQKREKKLILKGMLSRLVHLESWHGTLTGFKVENGLDGNVSERGDG
                     YEMVIRGLSVDQLIKVAGFIKQL"
     gene            4398..>4554
                     /locus_tag="ON345_003644"
     CDS             4398..>4554
                     /locus_tag="ON345_003644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073634.1"
                     /translation="MNEVLSDRKNKGNVTYRIVVSEDATRLFIELEKLMKVRSKEADK
                     KTTKKIVF"
BASE COUNT         1395 a          971 c          956 g         1232 t
ORIGIN      
        1 aaacgttgcg gaatggcgtc agagacgtca ttttgcggcg tttgccctat cctgcatcgc
       61 agtgaaaaga actcgcctcg tgcgctatga ttaaccgatg ataaaaggct gaaagaagca
      121 ctgcaagccg actggataac attgttgctt cacggctgat catcattggt tgcctcagtg
      181 ctagtgtgct ttgcatcagg aaccagaaga cgataccacg gcattttttt gttaagcagc
      241 tccatcaggg gagtggcaaa caaactcaat ccccacagcg ttacgattac tggaatcgtg
      301 aaattttgcg gcgtccagaa ctggcgctca aggtggaacc atgtaacaac acagagcatt
      361 ccaccaatcc agaaaatagc tttactgaaa gcccccagga ctgccagaga aataacagag
      421 actaaatgca gtgcgccagc cgtagcaaga cgtagataaa accagaacca tttagcgtgc
      481 cggataaccg ggttaccgtt cgtttctcgc aatcgttttt tacgttcccg gatgatttct
      541 tttttcagct tacttcgttc ggctccctgc ggaaactcat atattttagc catgttttgc
      601 ctcacttatt cttttttggt ggaacgactt tgtcaaactc ctctaaaaat gccatatcat
      661 cttccgaaaa ggatgaggat ttttcattta tactaaactc aaatctaagc atgtgagcca
      721 ttctcccttt tttcccaaca acagagaaag ataactgatt tatttccgtt ttctcaatta
      781 tctcattaat gcttttattg ataacatcac gaacaaaaaa tgagtattta ggatacttat
      841 atttcttctc cccttcctca atagtataaa gtcccatttc ctcttttaaa tcatccacgg
      901 atatatcaaa agattttttt gaactattct gactgtaata tttcctaatg acttgataaa
      961 ggtttgttga gtttacactg ttcaacaaca caactgattt caatacctga gtggtatatc
     1021 tattttcaga accaatcaac ttagaaaaat atcgcttagc tgtatttgtg aattttatcc
     1081 gtacagttgc aagatgatcg taataggcac agaattctgt taagtttaaa tccaagcgat
     1141 caggtatttt attcttagat gatagtatcc caagttcatc agccaaattt tttaaatcat
     1201 ctctgtttaa aactaacgta gttgaagaaa gaactttagc tccctctttc agagctgcat
     1261 aagaggcata tttatctaaa gaagtagctt ttgaaaaaaa gtcagcatca acctccaaaa
     1321 tatcatcatc taacatttcc tttgtatcaa tctggcaaag agccagaaaa agcactcttt
     1381 ttgctgaaag ggaaagggat gcaagagtta ccccaacttc atttctatgt ctaactttcg
     1441 ttaatcccgt tactgtcttc acgataagcc tcatataacc tttcctatgt aggcaaatca
     1501 taaccccaca aaggttgtaa ttcaagacaa caatacaacc tttaattaaa aaacctctca
     1561 cctttagata tgaagatctg aggatcttca tatctaaaga tagcaacaac cgcatgtttc
     1621 acttgatgtt cagtcaaact gtggaaaccg ttaaaaatct tctcacctta cgtgaaaaat
     1681 cctctcatct ttagtgaaaa atctaactac cttaacttaa aaatcttctc accttacgtg
     1741 aaaaatcctc tcaccttacg tgaaaaatcc tctcaccttt agcaattttt ttccataatt
     1801 ttcaggtaat tgaagcgagc taagttttta agtttttaag atcaagaaga aaatataagg
     1861 cttttaatgc acagcgtctg tggataactc agcataaatc cagaggatag atccgtctgg
     1921 cctccctgag cactgcacca ccaaaaattg agcaaatatt aaccttagcg cgtaagtgct
     1981 ctattgaaac cccgtggaaa ttccgcttcc tcgctcactc gcgggcttcg ccctgtcgtt
     2041 cggctgcggc gagcggtgta atctctctaa aagatccaga cgggatcctt ttccgatcca
     2101 attcttaaat gctctcaaaa gccctcagag gcgttttaac cgctttaacc cggtcatggt
     2161 tcaacggagc gggtaaaatg cgcttagaac gtcatacagc tcgtttttaa cctgatccct
     2221 aatcaggggg tataacagag gcggccaaaa aatccggcct tgctcgtcag agtatctacc
     2281 gcgcattatc cccgaacgga aacccgacca tcacaacact tgcacagctt gcagcagtag
     2341 ccgggttaca gttcactctc agtaagccca accactaacc tttcagacaa gcccctcata
     2401 ccggggggat tatttcttat gccagcaaaa tttcacgacc ggcgaggatg gccaagtgtt
     2461 ttttgctggc aattttcgtg cagcagctgg cgcccggatt cattttttgc tgtcaggcat
     2521 caatcggttt cagcaacccg ccccgtaatt cgctgctaac gcatctcata acgcgcagat
     2581 acagcgcccc gcgccagtgt ttcacactga cccggcgtac cgttcgcaag ctcacgcgct
     2641 aactgcgaca ggctaaacat cgtctgatag tcagaaattg gcctggcagg ggtgaggggg
     2701 gttatcgctc tcattcgggc ttgttggttc gccagtcctg gcgaacgtct ccggcgcgat
     2761 gttacgacag cgcattatgt aactacagtt cgcgcctctt tcggaaatcc cttcatgcgc
     2821 ggtaaagcaa tatacatctt tatgtaatct ttacaatgta aagcattaaa aatgattcca
     2881 taaccatcat ttgatggtat aatgaatttg ttggttggga atggttccct gccagattcc
     2941 agaagcccac acgggcggga gcgaaaaatg aatattcagg aagcattaaa cgtttttggt
     3001 ttatccggtg atctgactga aaaggacatc aaggcggcat acaaaaaagc ggctttaaaa
     3061 tatcatccag accgcaatcc attgggtgcc gagctgatga aagcagtaaa tgctgcgttt
     3121 gattttctca tggctaacat tgataaaatc aatcagtttc aaagcactga tgaaaacgca
     3181 cgttacaact acggtgaaga tctggaaaaa gttctgaata cgctttccgg ccttacaggg
     3241 attgtctatg aagttattgg taattgggtc tggattagcg gagaaactaa agaacacaaa
     3301 gacattttaa aggaaatggg ctgtaagtgg gcatctaaga aaaaacaatg gttttaccgt
     3361 cctgaagaac acaaaagccg ctggaaccgt aaagaacaca gcattgaaga aattcgtgaa
     3421 atgtatggta cagcgggtaa acgtaaggcg tcaggctgga cacgtgtaga agcaagcgca
     3481 taaccggaat gggggcgaaa gccccctaga acatggcaaa gattgattcg gagacacgtt
     3541 gatgaaaaac aagaccataa cggaagcgga gctgataaac atttttgaaa gttacggagc
     3601 ctacatttgc ccggatgaga tcgaagttac agccaaagaa tgcaatgaaa atggttcagt
     3661 cttgcaccgt ggcttaaacg cagaaggatg ggcgcatctt tttgcgaagg aagaagcata
     3721 tcagcaggaa tgcgaagccc aggaagctgc aagtgatgat ggacactttg atgaatagtc
     3781 ttaaaactgc cgtaattcga tttacggctt ttttattgag ttcaaaaggc tgaatcgatg
     3841 aaagaacaga aaaatttctt tgaacggtat aaaccggtgt ttgaaatcgt ttgtcgaatc
     3901 ctggggaacg gctggcgtgt aaatctgctc gatgattgcc agtacaggat taagctaacc
     3961 tcacctgatt ttaaaaatta ctctatacat attcgaatgg agaaaggaag gttagttatc
     4021 attggaagcg tagatagtcg cagctggaga agtccttacc atacttgcac agtatcgcca
     4081 gagcgtaacc ctgtagaaat cgccgccgat attgaaaaga aaatcctgtc ggatgcttta
     4141 gataatgtag atatggcgcg agagtatgaa caacagttac agcaaaaacg cgagaaaaaa
     4201 ctgatcctga aaggaatgct atctcgttta gtgcatcttg aaagctggca tgggacattg
     4261 accgggttta aagttgagaa tgggttagat ggtaacgtat cagaacgggg cgacggatat
     4321 gaaatggtaa ttcgtggcct gagtgtagat cagctcataa aggtagcggg gtttattaaa
     4381 cagctatgag gcaaaaaatg aatgaagtcc tgagcgacag gaaaaacaaa ggtaatgtaa
     4441 catacaggat tgttgtgtct gaagatgcaa cacgtttgtt tattgaactt gaaaaactga
     4501 tgaaggtacg ctctaaagaa gcggataaaa aaacaactaa aaagattgtt ttcc
//
LOCUS       DAJSKJ010000231         4451 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000231 DAJSKJ010000000
VERSION     DAJSKJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.235"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..212)
                     /locus_tag="ON345_003645"
     CDS             complement(<1..212)
                     /locus_tag="ON345_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR3073635.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIG"
     gene            complement(215..934)
                     /gene="sanA"
                     /locus_tag="ON345_003646"
     CDS             complement(215..934)
                     /gene="sanA"
                     /locus_tag="ON345_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR3073636.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFSALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1084..1968)
                     /gene="cdd"
                     /locus_tag="ON345_003647"
     CDS             complement(1084..1968)
                     /gene="cdd"
                     /locus_tag="ON345_003647"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3073637.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSSLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2098..2793)
                     /locus_tag="ON345_003648"
     CDS             complement(2098..2793)
                     /locus_tag="ON345_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR3073638.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2790..3188)
                     /locus_tag="ON345_003649"
     CDS             complement(2790..3188)
                     /locus_tag="ON345_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR3073639.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3427..4374
                     /gene="dusC"
                     /locus_tag="ON345_003650"
     CDS             3427..4374
                     /gene="dusC"
                     /locus_tag="ON345_003650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR3073640.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         1177 a         1131 c         1152 g          991 t
ORIGIN      
        1 atgccgatag ataaaattcc gaagccgata tatgccacca tataagcgaa aaagagtccg
       61 ggattttttg ctcgccagac atacaccggt tccggtgcat ttttaacccg tttatcaatc
      121 gcgcctgcgc gccagccttt ccatgcttcg cggaaacaaa atatcgggat aaggaaaaaa
      181 acggcaacga caaaaaactg ctgtacatcc ataactactt tccttgtttc ttttgtaatt
      241 caagtaactg ttcgggtgtg acggcgggat acccctgcgc atcttccggc acctggtgca
      301 tagccggaat agggaccagc ggccctaaaa aacgcggttc acgtttaaaa atataaaggt
      361 cagccagcgc actgaaacgg gcagcaaatt cgcgaatacg tactgacagc atatctttcg
      421 gtgacggtac ggcataacac tgagcctgaa tccccatatg cagcgcaata aataatgccc
      481 gctcacagtg gaaacgttgg gtgataataa tgaaatcatt agtatcgaac actttgcgtg
      541 tacgcacgat ggaatccagc gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt
      601 cgacaccagc tgcgattaaa tctttgcgca tggtcatcgg ctcattataa ctttgcaatg
      661 cgttatcgcc gctcagtaat agataattta ccttaccgct gttataggca ttaatcgctc
      721 cttgaatgcg gtagcgataa tactgattaa ttacgccagt acgataatat tttgctgttc
      781 cgagcaccac accgacctgg cggtagggga gatcctgcaa ttcgtcgtag atataaggcg
      841 cggttttcca gctcatccag cgatcgaggc cgagcacagt caacagcagc aagccgatca
      901 ggactaacag gctgaggaac acgcgcttta acatgcagga gactcgatct aaggaaaatt
      961 tccatcaggc tactttaccc gctggttttg cgcaagaaac ggcaaactgt ttgccgggaa
     1021 tttcgacacc aggagagggc tggcaagccg gacaaggcgt tcacgccgca tccggcacca
     1081 ggctcaagcg agaagcactc ggtcgatact gtgacagccg agagctttca gcgttgcgga
     1141 ggtggcatcc cactgaatca acggcgcatc ggctttttct gccagaaccg cgcgctggat
     1201 atccgggtaa tcataaccct tgagattcaa cagaattaac gctccctgca acggtggcag
     1261 agtcgggttg aatgcggcgt tttcagcgta gctgccactg aaaatacgac cgtctttaca
     1321 ttccagcgcg acaccgcttg gcgacttact gtaaggcatg tgcgaacggt ttgccgctgc
     1381 aatcgccgcc tgagaaagcg catcaccagt cagcgcatag ccgtgatcct gctcgtccat
     1441 cagcagcgtt ttaatctcca gatctttcgg cccaaaggca tccggcagat agtcacgcag
     1501 cgcgtgtgcc tcgcggcccg gcagatgaat acgcagatcc agactgctgt tcagttcatt
     1561 cataaactga cggcagtgac cacaaggcgt gtagttaacg gtgatggctg caagcgcttt
     1621 ttcaccactc aaccaggcgt ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat
     1681 tgtcgcacca ataaattcca tattggcacc gaaataccag gttccgctca caccgcgcgc
     1741 aattgcgcca acattaaaat tcgacaatgg cgtacgcgca caggccgccg ccagcggaag
     1801 tagtgcgaat gccagcgcgt cttcgtccag ccccgttgcg ctcttcagcg atgagacttg
     1861 ctccccggtc aacaaagcgg ggaagtactt gtctgccaga ataggttcca gtgcagattg
     1921 caagttatcc gcaagttggg caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca
     1981 taatcatgta atgggataac agtttacgaa cccgttatgg ctttatatgt gatctgaatc
     2041 tcattaatta tgcaacgcgt gaaacttaca tcaaaaatgc gcgacgcatc gcaaatttta
     2101 gcccattact gccagaataa tcgggaaaag gaacggtgcg atcagcgaag taattatccc
     2161 gcataacacc agcgctagcg aactaaatgc accttcctga taatccagct cggcgcagcg
     2221 cgccgtaccg agggcgtgcg aggcagtccc cattgccaga ccgcgcgcag ctttggtacg
     2281 aatacgcatc gcattaagca atgtatggcc aaataccgcg ccgaggatgc cgacgaaaat
     2341 cacgcaaact gcgctgattg ccggaatacc gccaatactg ccgccaaccg ccattgcaat
     2401 aggcgtggtg actgatttcg gcaggattga cgcggcgatt tccggtgaag cgcccatcaa
     2461 caatgccacg gaagtccccg tcaccatcgc aaccacgctg ccgataaaac agatggtgat
     2521 gatcgatttc cagcgcgcgc ggatctggtg tagctgctca tataaaggat aggccagcgc
     2581 gacgaccgcc ggttgcagca gatcgtttag cacttcgcta cctttaaagt agctgtcgta
     2641 agagatgccg gtcagcatta aaaaagggat gatcaccacc attgctacca gcaacgggtt
     2701 aagcaaggga aacttatacc gtgccgccag tttgcgggcg gcaaaaaaga caatcaatgt
     2761 cagtggtaat gaccaccaga tattcgccat cattcttctg atcctttctg acctacgact
     2821 ttacgttcac cgtgcaccag ttgcgaactc cagctcacca ccagaaaaac caccagcgta
     2881 ctgacagcac aggaaaccac taccgggcca aactgtgcgc ggagcaaatc aaaatattgc
     2941 atgacgccta cgccaatcgg cacaaacaat agcgccatat agcgaatcag tacgtagcac
     3001 cccggattga cccattttgc cggaagaatt tgcaaggcca gcaggacaaa caggatcagc
     3061 atcccgatga tgctgcccgg aatggttacc ggtagcaggg aagcaatgaa aatgcctgca
     3121 tacaggcagg cataaatcag gacgaaagcg cgtaaatatt gccagataat gttcagtgtc
     3181 ttactcataa tcaacacccc agatgagatg gattcatcat acaattaatt cgtaaaacgt
     3241 gctaccgatc acattaagaa atcagcgctt attttttcgc tccccggcat tacgcaatgc
     3301 gcaacgaact caggcataca tcaagcatag agagggattg cagtttgcag caatccctct
     3361 ttgtagacct ttaatccgct gaccataggc gctaacatag cgcctcatta ttttgcgggt
     3421 gatgatatgc gtgtgttact ggcaccgatg gagggagtgc ttgactctct ggtgcgtgaa
     3481 ttgctgaccg aagttaacga ctacgatctg tgcatcaccg agtttgtccg cgtggtggat
     3541 caactgctgc cggtaaaagt ctttcatcgc atttgccctg agctacaaaa cgccagccgg
     3601 acaccatctg gtacgctggt gcgcgtgcag ttgttaggtc agttcccaca atggctggca
     3661 gagaacgccg cccgtgcggt ggagttaggt tcctggggcg tggatctcaa ttgcggctgc
     3721 ccgtcgaaaa cggttaacgg tagcggcggc ggggcgacgt tactcaaaga tcctgaactc
     3781 atctaccagg gggcaaaagc gatgcgtgaa gctgtaccgg cgcatttgcc tgtcagcgtg
     3841 aaagtgcgtc tgggctggga cagcggtgag aagaaatttg aaatcgccga tgcggttcaa
     3901 caggctggcg ctacggagct ggtggtgcat gggcggacga aagagcaggg ttaccgcgcg
     3961 gagcatattg actggcaggc gattggcgag attcgccagc ggctgaatat tccggtgatt
     4021 gccaacggtg aaatctggga ctggcagagc gcgcaacaat gcatggcgat cagcggctgc
     4081 gacgcagtga tgattggtcg cggggcgctc aatattccca acctgagccg ggtggtaaaa
     4141 tataacgaac cgcgaatgcc gtggccggag gtggttgctt tgctgcaaaa atatacccgt
     4201 ctggaaaagc agggcgatac cgggttatat cacgttgcgc ggattaaaca gtggttgagt
     4261 tatttgcgta aagaatacga tgaagcaacg gaattatttc agcatgttcg ggtgttgaat
     4321 aattcccctg atattgcaag ggctattcag gcaattgata tcgagaaact ctaaaaaacc
     4381 atagtgatgt tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
     4441 ggcgtcaaaa t
//
LOCUS       DAJSKJ010000232         4414 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000232 DAJSKJ010000000
VERSION     DAJSKJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.236"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1733
                     /locus_tag="ON345_003651"
     CDS             <1..1733
                     /locus_tag="ON345_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR3073641.1"
                     /translation="NITLSKCVVSASKIEISPHCIPIQMIPSLSNARRKIFMTATLVD
                     ESILASHFGITDESLSNPITPKTIGDIGDRMILMPQVINPSLSDVDIKAMCKEISSKH
                     NVVVIVPSDYRAGFWQDVADRVLDKTTIYRGVQELRSQHVGLVVLVNRYDGIDLPNTA
                     CRLLVIDGLPDVRRLVDKVTQSLLLGSEKTKDEIIQKIEQGMGRGVRSSDDFCGVVLL
                     GKALNGAVFLGSSLDKFSPATKAQIQLSQQLVSILPDTTIDSIKGALDYCLLRKPEWA
                     SMSKGVLAGLTLEKKQIDQHIINKRLAYDLASRNMMQQAASTLINDSNTADKIYRGYL
                     KEHVAEYVNLYDKSEAQVVLQSASGDNYRVLKPLIGVTYNRLNGAALEQAKECSTYLR
                     GNFKAANQVVVYTNSVLENLIFSEGTSNPFEDAIDKVANLIGFRSQRPENDTGKGPDN
                     LWAMGENSYLVIECKNGASSERISKHDCNQLNGSGAWFRSMYDQTATFSPVMIHHSNM
                     PEYAATLNDGSRIMTIKDLDNFKASILNFITAICTSDKLHDEIFIRERLISCKLRASD
                     IVDTYTRRFR"
     gene            complement(1926..4199)
                     /locus_tag="ON345_003652"
     CDS             complement(1926..4199)
                     /locus_tag="ON345_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR3073642.1"
                     /translation="MAVETRGRIAPSPPVLLPKDTDDNFVGAYPWNKPGKAIGRDRPL
                     TRAELRQVQGVLNRISRLPFFLQTLFTSRYNFVRRTKSPLGGLYFLKNTFERKLLPRL
                     ERVNELCGMNQSASIGFLSERDEYARLPDMNDKELKKFAARIAAQLWNKYEELSDAWA
                     EAHGGKETLFTDEAQAHLYGEVAGVARAFNLTPMYWKKYRKGQMTIRQAFSAISRLIK
                     DEWWVNQLKAQRMRWREALLIAAGEVNKDRSPYASKMAIREVHSRRLANLEYLKSCEL
                     ENKVTGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAASVGDVGMFITLTTPSKY
                     HPTRQVGKGDSKTVQLNHGWNQTAFTPKDGQRYLCRIWSLIRTAFKDNDLEVYGMRVV
                     EPHHDGTPHWHMMLFCKPGQRKDINEIMRRYALKEDGHEKGAAKQRFESRHLNQGGAA
                     GYIAKYIAKNIDGYALDGQLDHDTGKPLKDTAAAVTAWASTWRIPQFKPIGLPTMGAY
                     RELRKLPRGVSIASEFDDRVEAVRAAADEGEFDLYIIAQGGANMKRDAQAVRVARKVT
                     DEVNEYEEDIEKIVGIYAPHLGAHRVHVTRTAEWRIVPKVLAVEPLNLKSGSAAPRSP
                     VNNCGKLTGGDVPVMTPTPSEHAAAVLNLVDGGVIAWDDPEVVTALRGALKHDAPRLN
                     RQQRSGEPLKPHQIAPSGRMTKAERAQIPRIRFELVQQGITPQRWELQALARGATVTF
                     DGQKFSYPVADEWPGFS"
     gene            complement(4189..>4414)
                     /locus_tag="ON345_003653"
     CDS             complement(4189..>4414)
                     /locus_tag="ON345_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HCR3073643.1"
                     /translation="FQFILQEKKVAKSGKNAGKEWLDTVGYYPTINKLISGLVVHNIF
                     TGDARQFSDLEEQVEQLGQKCLEAFTANGR"
BASE COUNT         1182 a         1049 c         1034 g         1149 t
ORIGIN      
        1 aaaatataac attatcaaaa tgtgtcgtta gtgcatcaaa aattgaaata tcacctcact
       61 gcatacctat tcaaatgata ccgagcctat caaatgcgag aagaaaaata ttcatgacag
      121 ctacgctggt cgatgagtca atccttgcta gtcacttcgg tataacagat gaatcactat
      181 caaatcccat aacaccaaaa actattggcg atattggcga tagaatgatt ctcatgcctc
      241 aagttattaa ccctagttta tccgatgtag acatcaaagc tatgtgtaag gagatatcgt
      301 ccaaacataa tgttgttgta attgtaccct ctgactacag agctgggttt tggcaagatg
      361 ttgcagatag agtattagat aaaactacaa tttaccgcgg tgtacaggaa ctgagatcgc
      421 aacatgttgg tttagttgtc ctcgttaata gatatgacgg tatcgattta cccaatactg
      481 catgccgctt acttgtaata gatgggcttc ctgacgttcg cagactcgta gataaagtca
      541 cgcaaagtct gttattagga agtgaaaaaa ccaaggatga aatcattcag aaaattgagc
      601 agggaatggg ccgtggggta cgatcaagtg acgacttctg tggtgtagtt ttactaggaa
      661 aggcattaaa tggtgccgtt ttccttggtt cttcgttgga taaattttct ccagcaacaa
      721 aagcacaaat tcaactctct cagcagttgg tatcaatatt gcccgatact acaattgatt
      781 caattaaagg tgctctcgat tattgcttat tacgaaagcc tgaatgggca tccatgagca
      841 aaggtgtatt agcaggtttg acgttagaaa aaaaacagat tgaccagcac ataataaaca
      901 aaagattagc atacgaccta gcgtcaagaa atatgatgca gcaagcagcg tctacgttaa
      961 taaatgacag caacacagca gataaaattt acagaggtta tttaaaggaa catgtagcag
     1021 aatatgtaaa cctctacgac aagtcggaag ctcaagtagt attacagtct gcatcaggtg
     1081 acaattaccg cgttttaaaa cctctaattg gcgtaaccta taatagactc aatggtgcgg
     1141 cattagaaca agccaaagaa tgcagcacct atctcagggg taactttaaa gcagcaaatc
     1201 aggtcgttgt gtatactaat tcagtattag agaatcttat tttctcagag ggtacatcta
     1261 acccatttga agatgctatt gataaagtgg caaatttgat cggattccgt agtcaacgac
     1321 cagaaaacga tacaggaaaa ggaccagaca acttatgggc aatgggtgaa aacagttacc
     1381 tagtaattga gtgcaaaaat ggcgcatcct cagaaagaat aagtaagcat gactgcaatc
     1441 agttaaatgg ctcaggtgca tggttcagaa gcatgtatga tcaaacagcc acttttagtc
     1501 ccgtgatgat acatcactct aatatgcctg aatatgctgc aactttaaat gatggctctc
     1561 gtataatgac aatcaaagac cttgataact ttaaagcatc tattcttaac tttatcacag
     1621 caatttgtac aagtgacaaa ttgcacgacg agatatttat aagggaacga ctaatttcat
     1681 gtaaattacg cgcatcagat atagtcgata cctacacaag aagatttcga taaacaatat
     1741 gtaaagccag cactcataag tgctggcttt aatttaaaat caatactttg aatgccatta
     1801 ttcattttcg tcactgctag gttttgctcc gcttttaaaa agcccgctaa caacaaaccc
     1861 attagaccaa ctatgcttac agtcgtcgtc cccatcaatg cgacttgcaa cgatcccctt
     1921 tcagattagg aaaatccggg ccactcatca gcaaccggat aagagaattt ttgcccgtca
     1981 aatgtcacgg ttgcgccacg cgccagcgcc tgaagctccc agcgctgcgg cgtgatgccc
     2041 tgctgcacca gctcgaagcg gatgcgcgga atttgcgccc gttctgcctt ggtcattcgc
     2101 cctgatggcg ctatctggtg cggttttagt ggctcgccgc ttctttgctg gcgatttagg
     2161 cgcggtgcgt cgtgtttcaa tgcgcctctg agcgccgtca cgacttcggg gtcatcccac
     2221 gcgataaccc cgccatcaac gagatttagc accgctgcgg catgctcaga cggtgtagga
     2281 gtcataaccg gaacgtcacc gccggtgagc tttccacagt tattgacagg actccgaggc
     2341 gcggcagagc cgctttttaa gttcaaaggc tcaacggcca aaacctttgg aacgatgcgc
     2401 cattcggctg tgcgggttac gtggacgcgg tgagcgccta gatgaggggc ataaatacca
     2461 acaatcttct caatatcttc ctcgtactcg ttgacttcat ccgtcacttt acgagcgacc
     2521 cttacggcct gagcatcgcg tttcatgttt gccccgccct gagcgatgat gtagaggtca
     2581 aattccccct catctgcagc agcccggaca gcctcgacac ggtcgtcaaa ctcgctggcg
     2641 atactcaccc ctcgaggcag tttgcgcagt tcgcggtaag cgcccattgt cgggaggcca
     2701 atcggtttaa actgagggat gcgccatgta gacgcccatg ctgttacggc tgcagccgta
     2761 tctttcagag gtttgccggt gtcgtggtcg agctggccgt caagcgcgta gccgtcgatg
     2821 tttttggcaa tgtatttggc gatgtaaccc gccgcgccgc cctgattaag gtggcgtgac
     2881 tcaaagcgct gtttagccgc gcccttttcg tgtccatcct ctttgagggc atatcgacgc
     2941 atgatttcgt tgatgtcttt acgctgaccg ggtttgcaaa acaacatcat gtgccagtga
     3001 ggcgttccgt cgtggtgcgg ctcgacaaca cgcatcccat acacttctaa atcgttatct
     3061 ttgaacgcag tacggatgag gctccagata cggcagagat agcgctggcc gtcttttggt
     3121 gtaaatgcgg tttggttcca gccgtgattg agctgcacag ttttgctgtc acctttgccg
     3181 acctgtcggg tcgggtgata tttcgacggt gtggtcagcg tgataaacat tcccacatca
     3241 ccaacactgg ccgcgtagcg ttcaatcccg gcgatagtat tcatcagctc catacgacga
     3301 atttcaggat ttgaaatact ccccatgact ttactgatga ggtcaatacg ttcgccggtg
     3361 actttgtttt ccagttcgca ggatttcagg tattcgaggt tagccaggcg gcgcgaatga
     3421 acctcgcgga tcgccatttt actggcgtag ggagaacggt ctttgttgac ctcaccggca
     3481 gcgataagca gcgcctcacg ccagcgcata cgttgcgcct tgagttggtt tacccaccac
     3541 tcatctttga tcagacggga aatagcggaa aatgcctggc ggatcgtcat ctgaccctta
     3601 cggtattttt tccagtacat cggggtgaga ttaaaagcgc gcgcaacacc ggccacctca
     3661 ccgtataagt gcgcctgcgc ctcatcagtg aaaagcgtct ctttgccgcc gtgagcctca
     3721 gcccatgcgt cactgagttc ttcgtatttg ttccagagct gcgcggcaat tctggccgcg
     3781 aatttcttca gctctttgtc attcatatca ggcaggcgcg catattcgtc acgctctgac
     3841 agaaacccaa ttgaggctga ttggttcatc ccgcacagct cgttaacgcg ctcaagacgc
     3901 ggaagcagct tccgctcaaa cgtgtttttg aggaaataaa gcccacctaa cgggctcttt
     3961 gtgcgacgaa caaagttata acgcgaggta aacagtgttt gcaggaaaaa cggcaggcgg
     4021 gagatccggt ttaaaacacc ttgcacctga cggagttcgg cacgtgtaag gggtctgtcg
     4081 cggccgatgg ccttgccggg tttgttccac ggataagcac cgacgaaatt atcatcggtg
     4141 tcttttggta gtagtacggg tggcgagggg gcaatgcgcc cccgagtctc aacggccatt
     4201 agcggtgaat gcttcaagac atttttgacc taactgctcg acctgctctt ctaagtctga
     4261 aaattgacga gcatcacctg taaaaatatt gtgtactacc agacccgaaa tgagcttatt
     4321 aatagtggga tagtaaccaa cagtatcgag ccattcttta ccggcgtttt tcccggactt
     4381 agcgactttc ttttcctgca aaataaactg aaat
//
LOCUS       DAJSKJ010000233         4328 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000233 DAJSKJ010000000
VERSION     DAJSKJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.237"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(64..822)
                     /locus_tag="ON345_003654"
     CDS             complement(64..822)
                     /locus_tag="ON345_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3073644.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIECVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="ON345_003655"
     CDS             complement(830..1933)
                     /locus_tag="ON345_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3073645.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMSIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /locus_tag="ON345_003656"
     CDS             complement(1943..3124)
                     /locus_tag="ON345_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3073646.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSIYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFADTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4217)
                     /locus_tag="ON345_003657"
     CDS             complement(3192..4217)
                     /locus_tag="ON345_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3073647.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKGGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1189 a         1140 c         1018 g          981 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgcagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgca ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacga
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggtagggcct gtaatccgcc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcgac atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agagcaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag accgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gattgacaca aggccaatca
     1621 gtaatgccag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgtcagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagatg tataaatcga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggaaagct
     2761 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag
     2821 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     2881 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt actgagattc gttacagtgt
     3001 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3061 aggcacgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac
     3181 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc gaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccaa cttcagatcc
     3361 ttgccgtaat cgcctccttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3541 ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct taatttgatg
     3601 cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat attgtttgcc
     3721 ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccagt aaatgccatt cccatcccgg cgtcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacttcc cctgactgta aagccgtgaa gcgttctttt
     3961 gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4021 cgacaaacat caacatcaat acctgaaaac ttaccgtctg catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataaac aaaagtagtg ccagcttttg
     4321 cgcccgat
//
LOCUS       DAJSKJ010000234         4317 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000234 DAJSKJ010000000
VERSION     DAJSKJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.238"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            358..906
                     /locus_tag="ON345_003658"
     CDS             358..906
                     /locus_tag="ON345_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3073648.1"
                     /translation="MIYGYARVSTNHQDTELQLTALKSAGCEKIFEEHASGRKSNRPV
                     LKRLIATMQPGDELVVWKLDRIGRNVLHALLMFQQLQEKGINFRSITDGVDLKTASGR
                     YNFRNILSAAQYESDLNSERTLAGLAVARAKGRVGGRRPKFTDEQWREMGERMATGES
                     RQSVSKTYGVGLSTLYKKFPAS"
     gene            complement(1025..1321)
                     /locus_tag="ON345_003659"
     CDS             complement(1025..1321)
                     /locus_tag="ON345_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR3073649.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1545..2264
                     /gene="tonB"
                     /locus_tag="ON345_003660"
     CDS             1545..2264
                     /gene="tonB"
                     /locus_tag="ON345_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR3073650.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(2304..2702)
                     /gene="yciA"
                     /locus_tag="ON345_003661"
     CDS             complement(2304..2702)
                     /gene="yciA"
                     /locus_tag="ON345_003661"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR3073651.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(2807..3346)
                     /locus_tag="ON345_003662"
     CDS             complement(2807..3346)
                     /locus_tag="ON345_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR3073652.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3376..4119)
                     /locus_tag="ON345_003663"
     CDS             complement(3376..4119)
                     /locus_tag="ON345_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR3073653.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASASILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4147..4236)
                     /locus_tag="ON345_003664"
                     /pseudo
     CDS             complement(4147..4236)
                     /locus_tag="ON345_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         1245 a         1032 c         1050 g          990 t
ORIGIN      
        1 cgcaaccaaa agtcgaagtc gaacaaattg tacacgccaa gatggtaact agggggaagg
       61 atatcgcttt cagtgtatat tgcaacaaaa atttggagcg taacaatatt gaggttgatt
      121 gatgtggatt taaataaaat catttatgta ccacttaggt tcagagaatc tcccgaatgt
      181 caaacactcc gccttagaag gacgcgcgtg ggggggggcg cgggctgata cagaagcgaa
      241 gatccactca gcattatttc ctgcatgggt tcatttcata tccaaccttt tttaaaaccc
      301 tcaagcatct ggcgatctct cgccgtttct cctgttttta caacaggaga agcactcatg
      361 atttacggtt atgcccgagt atcaacaaac caccaggaca ctgaattgca actaacggcg
      421 ctcaagtcag cgggttgtga gaaaattttt gaagagcatg ccagcgggag gaaatcgaat
      481 cggccggttc taaaacggct gatcgccact atgcagccgg gggatgaact ggtggtctgg
      541 aagctggaca ggataggccg caacgttctg catgcgctgt tgatgttcca gcagttacag
      601 gaaaagggta tcaacttccg cagtattacc gatggcgtgg atctcaaaac agccagcggc
      661 cgctataact ttcgtaacat cctttccgca gcacaatatg aatctgatct taatagcgaa
      721 cgtaccttag cagggctggc cgtagccagg gcaaaagggc gagttggtgg tcgcaggcct
      781 aagttcacgg atgagcaatg gcgggaaatg ggggagcgga tggcaaccgg tgaatcacga
      841 caaagcgtat caaaaacgta tggagtaggg ctctcaactc tgtataaaaa gtttccagct
      901 agctgataac gggaaagaaa cagagaaggg cacaaatatt gtgtacttta atgtgccctt
      961 taatttattg attggtggtt gaattgtccg taactttttg atttaagtgc aaatttctaa
     1021 taaattagaa cactttctta aatggtttca ctgaaacgtg ttcatagaca cctgccgcta
     1081 cgtacgggtc agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa
     1141 cggttgagcc agtaaatccc gcagcccctg gatcgttact gtctaccgct ggcattggac
     1201 cagctgtcaa caaacgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac
     1261 gaacggaaag gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca
     1321 cgggcacaac tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct
     1381 gcaacggaaa gatgatgtct ttgttaagac catgcataaa gtaaggtcaa ttacgccaaa
     1441 aatgacattt tcactgatcc tgatcgtcat gccttattga atatgattgc tatttgcatt
     1501 taaaatcgag acctggtttt tctactgaaa tgattatgac ttcaatgacc cttgatttac
     1561 ctcgccgctt cccctggccg acgttacttt cggtctgcat tcatggtgct gttgtggcgg
     1621 gtctgctcta tacctcggta catcaggtta ttgaactacc tgcgcctgcg cagccaattt
     1681 ctgtcacgat ggttgcgcct gctgatctcg aaccgccaca agccgttcag ccgccaccgg
     1741 agccggtggt agagccagaa ccggaacctg agccgatccc cgaaccgcca aaagaagcac
     1801 cggtggtcat tgaaaagccg aagccgaaac ctaagccaaa accgaagccg gtgaaaaagg
     1861 tacaggagca gccaaaacgc gatgtcaaac ccgtagagtc gcgtccggca tcaccgtttg
     1921 aaaatacagc acccgcgcgc cccacatcaa gtacggcaac ggctgcaacc agcaagccgg
     1981 ttaccagtgt ggcttcagga ccacgcgcat taagccgtaa tcagccgcag tatccggcac
     2041 gagcacaggc attgcgcatt gaagggcagg ttaaagttaa atttgacgtc acgccggatg
     2101 gtcgcgtgga taacgtacaa atcctctcag ccaagcctgc gaacatgttt gagcgtgagg
     2161 tgaaaaatgc gatgcgcaga tggcgttatg agccgggtaa gccaggcagt gggattgtgg
     2221 tgaatatcct gtttaaaatt aacggcacca ccgaaattca gtaagcagaa agtcaaaagc
     2281 ctccgaccgg aggcttttga ctattactca acaggtaagg cgcgaggttt tccttcagga
     2341 tcaaccgcga catacttaaa taatgcttct gtcgctttat agcgttgccc gattggttca
     2401 gacgctactt ttttcaccca cacttcaata ttaatgctga ccgatgtcgt ccctttctgg
     2461 acacagcgtg catagcagca caccacatcg ccgaccgcaa ccggccgtaa gaaagtcatt
     2521 ccttcaaccc gcacagtcac tacgcgaccg tgggcaattt ctttcgccag aatagcgccg
     2581 ccaatatcca tttgtgacat taaccaacca ccaaagatgt caccattggc attggtatcg
     2641 gcgggcatgg ctaaagtacg taaaacaaga tcgccctgag ggacgttatg tgttgtagac
     2701 atggtaaaac cgacttaaaa ggaaaatcac aggcgcgatg ctactatgat tttcctgagg
     2761 agaacagagg agatacagcg ccagccccga aggactggca gtctggttag gatttatctt
     2821 cctgcggcat gtggcggtag atatagatac cgcttaacaa tgtgaagatt aaggtaaggg
     2881 cggtcaggcc aaagacttta aagttgaccc aaatattttg cggcagccag aatgcgatgt
     2941 agatgtttgc cagaccgcaa aggataaaga aaacagccca ggccagattt agcttcgacc
     3001 ataccggttg cggcagcgtg agttctttac ccagcatccg ctgaattagc ggctttttca
     3061 tcacccattg gctgactaac agggctcccg caaacagagc ataaatgact gtaaccttcc
     3121 atttaataaa ctcatcattg tggaagaaca gcgtcaagcc accgaagacg accaccagaa
     3181 caaaagtgat cagggccatc ttctcaacct tacgaaagcg aacccagcta tagataagca
     3241 caatcgccgt ggcgacgatc agcgccgcag tagccgcata gatgtcataa atcttgtaaa
     3301 acgcgaaaaa gacaaccagc ggtaaaaaat caagaaactg cttcatttta cgattccgta
     3361 atcaaatgct taaggttatt ggcgaatcaa catatacagg cggaacagat agatgagcaa
     3421 tatggcagaa atcaggttgc tcaaggtgtt cgccagtacg gcaccaattt ccggggttaa
     3481 tgcggcaaaa gaagaggcaa aaagcagcaa cagtgttttt gccagcaacc agctcagtac
     3541 tgcgggtgcc accagacgca tattcgccca agtcagccgc atactgctac gcatcgaggc
     3601 aaaaatgccc attttgtcct gtaccagcat caccggtgcc agggccagta aaatggccat
     3661 gataattccc ggaacgacca ccagcatgat gccaatctgt actaaaaggg tagtcagaaa
     3721 aatcagaata aataacttgg gcaatatcga cgcactggca ccaatagctc gcagcgcact
     3781 gactctctga cccgcagaca ccagctggat aattaatatt acgcctccgg cgagaatggc
     3841 gttaccgatt aatcctgaaa aagtggacgc cgctgaagcc tgcagcaaaa tttgttgctg
     3901 ttccggtgac atattctgaa ccaggtcgaa caacccacta ctgccgctaa cgggcacgcc
     3961 gtcattgagc tgcgcaagct gtgcatcact gggtgagaaa acatgcccta acaccactgt
     4021 gataaacgca catagcaacg ataccaacag aatggtcata aattgattac ggaagaaatt
     4081 tccggtgtca cggtatacgg actgcgccgt gatagacatg cactctcctt gagttttatg
     4141 caggtgttaa ttagcgggca attgtaccct ggttaagcct cgggtggcag catcaggtct
     4201 tgtatggaaa agcatatctt tgtaaagcgg aggtaattgc tggccttcag gcgtgggcaa
     4261 accgcggcaa gtggcgatcg agttaatatt tgcgtaaaga aaatatttaa aaattga
//
LOCUS       DAJSKJ010000235         4288 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000235 DAJSKJ010000000
VERSION     DAJSKJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.239"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            32..562
                     /locus_tag="ON345_003665"
     CDS             32..562
                     /locus_tag="ON345_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR3073654.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1132..1221)
                     /locus_tag="ON345_003666"
     tRNA            complement(1132..1221)
                     /locus_tag="ON345_003666"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1185..1187),aa:Ser,seq:cga)
     gene            1315..2112
                     /gene="mtfA"
                     /locus_tag="ON345_003667"
     CDS             1315..2112
                     /gene="mtfA"
                     /locus_tag="ON345_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR3073655.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2213..2288
                     /locus_tag="ON345_003668"
     tRNA            2213..2288
                     /locus_tag="ON345_003668"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2246..2248,aa:Asn,seq:gtt)
     gene            2602..>4288
                     /locus_tag="ON345_003669"
     CDS             2602..>4288
                     /locus_tag="ON345_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HCR3073656.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAA"
BASE COUNT         1159 a         1016 c          979 g         1134 t
ORIGIN      
        1 cttgactgta aaaattacag gagctacaaa aatgaaccga ttctcaaaaa ctcaaattta
       61 tttacattgg ataacgctgc ttttcgttgc aataacctat gccgcgatgg aactccgtgg
      121 ctggtttcct aaaggtagta gtacttatct gctgatgcga gaaacacatt acaatgcggg
      181 tatattcgtt tgggtgttaa tgttttcacg cctgattata aaacaccgtt atagtgatcc
      241 ttctattgtg ccaccgccac ctgcctggca aatgaaagcg gcttcgctaa tgcacatcat
      301 gctttatata accttccttg cacttcctct gctggggatt gctttgatgg cttacagtgg
      361 aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt gttaccccaa acagcgaaat
      421 taaagcactg ataaaaaata ttcacgaaac ctgggcaaat ataggttact ttttaatcgc
      481 agctcacgct ggcgcagcac tctttcatca ctacattcag aaagataata ctctgttacg
      541 aatgatgcct cgccgcaaat aagtcgatcc cctgacaagg aaatgtgaac catggacatc
      601 aacgcgtcat aaatgtttac gcaagtgcac aatgcaccct ttccactatc aacaaggagg
      661 agcagaaact gtgtaaatcg tacaatggta agacttatca aaaatacagg ttaattttgg
      721 tatttaaacc ttttttactt gttagtatga tacaaaggct ttcaaaaaag ctgggaaatt
      781 caacaactct tggaaattat ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct
      841 tttttgactc aattattgaa cccttctcta cgcaacttca gttcccacca ccaactttgc
      901 ggcagtttca taggatgaat gtctaaaaga ataatggtga gcgataagaa aacgactgaa
      961 taactgcaca ttttcgctcg aaagcttcct gtcagatcca tagcgaatca agtgctgaat
     1021 gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt ctacgattgt
     1081 tctgtttagg aaaagcaagg cgggaagtcg ggagataagt cattgataaa gtggcagaga
     1141 gagggggatt tgaacccccg gtagagttgc ccctactccg gttttcgaga ccggtccgtt
     1201 cagccgctcc ggcatctctc cgttcagatg gttgccatga tgccaggaaa tttggcattt
     1261 taacagtccc tgtccgtgca attttgttca agtgacgagt ttgcgagcaa aacgatgatt
     1321 aagtggccct ggaaagtaca agaatcagca catcaaactg cccttccctg gcaggaagca
     1381 ctatcgatcc cccttttaac gtgtctgacg gaacaggaac aaagcaaatt agtcactctt
     1441 gccgaacgtt ttttacagca aaagcggctt gttcctttac agggctttga actggattca
     1501 ttaagaagct gccggatagc acttctattt tgcctacccg ttctggagtt aggactggaa
     1561 tggctggatg gttttcatga agtcttaatt tatcctgcgc catttgtggt cgatgatgaa
     1621 tgggaagacg atatcggtct ggtgcataac caacgtattg ttcagtcagg tcagagctgg
     1681 cagcaagggc ctatcgtttt gaactggttg gatatacaag attcttttga tgcttctggt
     1741 tttaacctga ttattcatga agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc
     1801 agcggagttc cctttattcc gttgcgtgag gttgctggct gggaacacga tcttcatgct
     1861 gcaatgaaca acattcagga agaaatcgaa ttggttggcg agaatgcggc gagcattgat
     1921 gcttatgcag ccagcgatcc tgctgaatgt tttgccgtac tttctgaata tttctttagc
     1981 gccccagaac tttttgctcc tcgtttccct tcattgtggc aacgtttctg ccaattttat
     2041 caacaagatc ctttgcagag actgcatcac gctaatgata cagactcgtt ttcggcgacg
     2101 aatgttcatt aattaacaac tttgcagatt aattaaccaa ttgaaatgac ttatgaaatt
     2161 tagtgttgac agacaaggta ccgctaagta atattcgccc cgttcacacg attcctctgt
     2221 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     2281 aggagccaaa ttctaaaaat tcgctttttt agcgcaatgt cactgacctt agttgaacat
     2341 tgttttttaa cggatagcgg gtttttaaca tcttaagcgc cctcgacctt tatggttgag
     2401 ggcgttttgc tatgaacgcc atcaccattt tcccctcgat tataaaactt gggttattca
     2461 gtagtctccc ctcttgcaac tcacacccaa aactgcctaa cgaaaagtta ttaattttca
     2521 atcatattgc tatcagtatt tacatttttt cgctgtgcta gaaagggcgc atttatgtta
     2581 gctcgttcag ggaaggtaag catggctacg aagaagagaa gtggagaaga aataaatgac
     2641 cgacaaatat tatgcgggat gggaattaaa ctacgccgct taactgcggg tatctgtctg
     2701 ataactcaac ttgcgttccc tatggctgcg gcagcacaag gtgtggtaaa cgccgcaacc
     2761 caacaaccag ttcctgcaca aattgccatt gcaaatgcca atacggtgcc ctacaccctt
     2821 ggagcgttgg aatcggccca aagcgttgcc gaacgtttcg gtatttcggt ggctgagtta
     2881 cgcaaactca accagtttcg tacgtttgct cgaggttttg ataatgtccg ccagggtgat
     2941 gaactggatg tcccggcaca agttagtgaa aaaaaattaa ccccgccgcc gggtaatagc
     3001 agtgacaacc tcgagcaaca gatagccagt acttcacagc aaatcgggtc tctgctcgcc
     3061 gaagatatga acagcgagca agcggcaaat atggcgcgtg gatgggcctc ttctcaggct
     3121 tcaggcgcaa tgacagactg gttaagccgc ttcggtaccg caagaatcac gctgggcgtg
     3181 gatgaagatt ttagcctgaa gaactcccag ttcgattttc tccatccgtg gtatgaaacg
     3241 cctgataatc tctttttcag tcagcatact ctccatcgta ctgacgagcg tacgcagatt
     3301 aacaacggct taggttggcg tcatttcacc cccacatgga tgtcgggcat caacttcttt
     3361 ttcgaccacg atcttagccg ttaccactcc cgcgccggca ttggcgcgga gtactggcgc
     3421 gactatctaa aattaagcag taacggctat ttgcgactga ccaactggcg cagcgcacct
     3481 gaactggaca acgattatga agcacgcccg gccaatggct gggatgtacg cgcagaaagc
     3541 tggctacccg cctggccgca ccttggcggt aaactggtct atgaacagta ttatggcgat
     3601 gaagtggccc tgttcgataa agacgatcgg caaagtaatc ctcatgccat aaccgctgga
     3661 cttaactata cccccttccc gctgatgacc ttcagcgcgg agcaacgcca gggtaaacag
     3721 ggcgaaaatg acacccgttt tgccgtcgat tttacctggc aacctggcag cgcaatgcag
     3781 aaacagcttg acccgaatga agtcgctgca cggcgtagcc ttgcaggcag ccgttatgat
     3841 ctggtggatc gcaacaacaa tatcgttctg gaatatcgca aaaaagaact ggttcgcctg
     3901 accctgacag accccgtgac agggaagtca ggagaagtga aatcactggt ttcgtcgcta
     3961 caaaccaaat atgccctgaa aggctataac gtcgaagcca ccgcactgga agctgccggt
     4021 ggcaaagtgg tcacaacggg taaagatatt ctggttaccc tgccggctta ccggttcacc
     4081 agtacgccag aaaccgataa cacctggccg attgaagtca ccgccgaaga tgtcaaaggc
     4141 aatttgtcga atcgtgaaca gagcatggtg gtcgttcagg cacctacgct aagccagaaa
     4201 gattcctcgg tatcgttaag tacccaaaca ttgaacgcgg attcccattc aaccgccaca
     4261 ctgactttta ttgcgcatga tgcagcag
//
LOCUS       DAJSKJ010000236         4283 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000236 DAJSKJ010000000
VERSION     DAJSKJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.240"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..1487)
                     /locus_tag="ON345_003670"
     CDS             complement(<1..1487)
                     /locus_tag="ON345_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR3073657.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPRVIGHEF
                     FGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNVCTTLAVLGVHADGGFSE
                     YAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTENDTVL"
     gene            complement(1693..2019)
                     /locus_tag="ON345_003671"
     CDS             complement(1693..2019)
                     /locus_tag="ON345_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR3073658.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPTGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2154..2495
                     /locus_tag="ON345_003672"
     CDS             2154..2495
                     /locus_tag="ON345_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR3073659.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2530..3090
                     /gene="speG"
                     /locus_tag="ON345_003673"
     CDS             2530..3090
                     /gene="speG"
                     /locus_tag="ON345_003673"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR3073660.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3093..3803)
                     /locus_tag="ON345_003674"
     CDS             complement(3093..3803)
                     /locus_tag="ON345_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR3073661.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKSSSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVSNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3911..4216
                     /locus_tag="ON345_003675"
     CDS             3911..4216
                     /locus_tag="ON345_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR3073662.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1173 a         1099 c          953 g         1058 t
ORIGIN      
        1 agaacggtat cattttcagt cggttgacca tgaccggtaa cgttagccgc aatggtaaaa
       61 ggttcgatca tcaccgcata ttgatcggcc actgcttcag gaattttcca cgcatttttt
      121 gccggaacca cggcatattc actgaaacca ccgtcagcgt gcacacctaa tacagcaagt
      181 gtcgtacaaa cgttcggctt acctatagag cacggatagc aatgcccaca gctgaccacc
      241 ggatcgacag caacgcgttc accgactctg gcgctttcca caccttcacc caccgcatca
      301 atgacgccaa agaattcatg accaatgacg cgcggatatt tcgccgccag cttttcatcg
      361 aattcgatgc caagacccgg tttgtctccc ggatgcatat agccgttatc gaaagtccag
      421 ttgtgcggga agacttcgag catttgttcg gaataaccca tgtactcctg gacaccgaaa
      481 ttggggaccc acagatcaaa gtgcagcgcc gcagccatgc agactggtga caaatcggaa
      541 ggaccgtgtg agccagtacg tacctgatac agcgaagcaa aatcggcaat ccggcgcata
      601 ccggtaattc cgcctgcatg ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca
      661 atcagttgtt tgcagtccca gatgctgttg aagacttcac ccactgcgat gggtgtgacg
      721 gtatgttggc gaatgagacg gaagcattcc tggttttccg caggcgtcgg gtcttccatc
      781 cagaacatgc gataatcttc aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag
      841 cgatggtgca tgtcatgcag caaatgttca ttaaaaccaa acttgttacg taccgcgtca
      901 aacaatttcg gcatgaaatc gaggtatttc tccgtcgacc acagctgctc ttccggccac
      961 tgtcctttgg ttgcgggttc ataagccaga cctttacctt tcgacatgcc gtaggtggtt
     1021 ttcataccag ggattccgca ctgcacgcgg atggctttga atcccagctc ctgatgacgg
     1081 gcataatcat ccagagcttc atcaatactg tgaccggtgg tatggcaata aaccatcacc
     1141 ccttcacgag acgcgccgcc gagtaactgg taaagcggca tgttggcagc tttggcttta
     1201 atatcccaca gcgccatatc aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc
     1261 cagtaagcac ctttatagaa aaactgccag atatcttcga tacggtgcgc atcgcgacca
     1321 ataagctgcg gacaaaggtg atcctgcaaa taagaggcca cggaaagctc acgtccattg
     1381 agggtggcat ccccaaggcc cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa
     1441 ttacgccccg gacaggtaac aaaaacttca gcctttacga tcttcatgtt cgattccttg
     1501 catcgcttgt cgtgatgcat gaaatctacg caactgagct actaccatac aagtataaag
     1561 atcgaaaaaa gccggagtga tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac
     1621 aaaagatgaa atagaatacc ttttgtcagc tgacacttcc tcgtatctta ttgataaaat
     1681 ggatttatgt tcctacgtgc gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc
     1741 aatcaacgca cccgtccagt cataaagact tagtttcacg ccatccacaa cgcgcagcca
     1801 catcaacgcc gtgcagacat aaacgccacc ataagccgcg taaacacgcc cactcgccgc
     1861 tggatgcaac gttaacaacc agacaaacag cgccagtgaa atccccgtcg gaagcaacag
     1921 ccagatactg gcgtttcgtt ttaaccacaa ccagggcaga aagcatccaa taatttcaca
     1981 cagcgcagta gcaaaaaata gtaacgttgt tttaatcatc tttgtcactt attgacatca
     2041 tgtatagtta tagggcgaca taatatcatc aatataaaca ccctcctggt acgttttgcg
     2101 ttcgcagtgg atggtgtaga atcaccttta atcattcata cagggaatga attatgaaaa
     2161 tcactctcag caaacgaatc ggcctgctcg cttttctgct gccttgcgca ctggcactga
     2221 gcacaactgt tcatgccgaa actaacaaac tggtgattga gtctggcgac agtgcacaaa
     2281 gccgccagca cgccgctatg gaaaaagagc aatggaatga cacgcgcaat ctgcgtcaga
     2341 aagtgaataa acgcactgaa aaagagtggg ataaagccga cgccgctttt gataaccgcg
     2401 ataaatgtga gcaaagcgcc aacatcaatg cctactggga gccaaatact ttgcgctgcc
     2461 tggaccgtcg aactggccgc gttattaccc cctaacctgt tattgattta aggaatgtaa
     2521 ggacacgtta tgccaagcgc ccacagtgtt aagctacgcc cgctggagcg tgaagattta
     2581 cgctatgtac atcaactcga caataacgcc agtgtgatgc gttactggtt tgaggaaccc
     2641 tacgaagcct ttgttgaact ctctgatctg tatgataagc atattcacga tcagagcgaa
     2701 cgacgctttg tggtggaatg tgacggcgaa aaagccggtc tggtggagct ggtggaaatt
     2761 aaccatgtac atcgccgggc agaatttcag ataattatct ccccggagta tcaggggaaa
     2821 ggtctggcaa cccgtgccgc caaattagca atggactatg gcttcaccgt tctcaatctc
     2881 tataagctgt atctgatcgt cgataaagag aatgaaaaag cgattcacat ttaccgcaag
     2941 cttggcttta cggttgaagg tgaattgatg catgagtttt ttattaatgg tcaatatcgt
     3001 aatgccattc gcatgtgtat attccagcat cagtatctgg cagagcacaa aacaccgggt
     3061 cagactctcc tgaagccgac cgcacaatag cattaataat aatcgatcgt atttttgatg
     3121 gtgtaaaccc gttcgacggc gggttttact ccttcatcaa caatgatcag ctgacagtcc
     3181 accggattgg cgtgactgtc atattcacag ctctgtttta cattactaac tcgttgatta
     3241 ttcagtaaag taaccgctgt gtaatctaat tttttgatcg ggtccgttga tggcgtggcg
     3301 ctgacagata atgttttgtc gttacttttc gtggttttac ccagtggata accctgatca
     3361 tcatagcgat attccatctg catttgtttg ctgctggatt taatcacgaa gccattatca
     3421 tcggtttccc aactcacccc ggcagaaggt aattctgcta gctggcattt tccctgtaaa
     3481 cgtactctct tctccagcgt ctcggcatca cggtaataat tggcgtccag taccagagcg
     3541 accacggtat tattttccag atccagtaat tcgagtgaat caaaacagcc ttcttccgac
     3601 aaagtcccag aaacacgttt cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa
     3661 tcttttaccg gaccacgcag cggatcaaaa tcgaattcat tagagaaact ggccatctca
     3721 ggggtaaatg aaagtgttac ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag
     3781 catggtagta atttgtattt cacaacagtc accagagagt agagatgatt ctcaatcata
     3841 gtagcaaata cagtacttta cacgttaaat gctatgctta aagaagttat cttcgcgtaa
     3901 ggagcttatg atgaaactct caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc
     3961 cgcggtactc gccgcgccgg gatcctgtga gcgcatacag agcgatatat cacagcgcat
     4021 tatcaataat ggcgtacccg aaagcagctt cacattaagt attgtaccca atgaccaggt
     4081 tgatcagcct gattcccagg tcgtcgggca ttgtgctaat gatacgcata aaattctcta
     4141 cacccgcacc accagcggta acgtctctgc tcccgcgcag tctacccagg atggcgcgcc
     4201 tgccgaaccg cagtaataca ttatcgactg aacgccggat acgacacatc cggcgatttg
     4261 aacatacaac ataatcccac ctt
//
LOCUS       DAJSKJ010000237         4229 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000237 DAJSKJ010000000
VERSION     DAJSKJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.241"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ON345_003676"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ON345_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3073663.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ON345_003677"
     tRNA            complement(324..399)
                     /locus_tag="ON345_003677"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ON345_003678"
     tRNA            complement(406..480)
                     /locus_tag="ON345_003678"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ON345_003679"
     tRNA            complement(597..681)
                     /locus_tag="ON345_003679"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ON345_003680"
     tRNA            complement(690..765)
                     /locus_tag="ON345_003680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ON345_003681"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ON345_003681"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3073664.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ON345_003682"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ON345_003682"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR3073665.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ON345_003683"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ON345_003683"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR3073666.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1096 a         1046 c          947 g         1140 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cacctgttta ctcacatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 aaatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcttgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgataca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgggggatg gcttgtagat atgacgacag gaagagtttg
     4201 tagaaacgca aaaaggccat ccgtcagga
//
LOCUS       DAJSKJ010000238         4142 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000238 DAJSKJ010000000
VERSION     DAJSKJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.242"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1460
                     /gene="mltF"
                     /locus_tag="ON345_003684"
     CDS             <1..1460
                     /gene="mltF"
                     /locus_tag="ON345_003684"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR3073667.1"
                     /translation="NRIAAIQARGELRVSTIHTPLTYNEINGKPFGLDYELAKQFADY
                     LGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYK
                     VGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVDDKKGSAELMEDVI
                     EGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNTLSAALLDFFNE
                     MNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAA
                     IAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKV
                     PESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQKPYYS
                     KLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTELGK
                     EKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN"
     gene            complement(1457..1993)
                     /gene="tadA"
                     /locus_tag="ON345_003685"
     CDS             complement(1457..1993)
                     /gene="tadA"
                     /locus_tag="ON345_003685"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR3073668.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(2018..2653)
                     /gene="yfhb"
                     /locus_tag="ON345_003686"
     CDS             complement(2018..2653)
                     /gene="yfhb"
                     /locus_tag="ON345_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR3073669.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     QLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2862..3710
                     /locus_tag="ON345_003687"
     CDS             2862..3710
                     /locus_tag="ON345_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3073670.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LMKKLV"
     gene            3766..4026
                     /gene="yfhL"
                     /locus_tag="ON345_003688"
     CDS             3766..4026
                     /gene="yfhL"
                     /locus_tag="ON345_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR3073671.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT         1095 a         1045 c         1025 g          977 t
ORIGIN      
        1 acaaccgtat cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc
       61 ccctgactta taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac
      121 agtttgccga ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc
      181 tgtttgacga ccttgataat ggtaacgccg acctgctggc ggcaggactg gtctataaca
      241 gtgagcgggt aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg
      301 tttataaagt gggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca
      361 ccgttgcacc gggtcatgtg gtggttaacg atctccagac cctgaaagaa acaaaattcc
      421 cggaattaag ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca
      481 tcgaaggaaa actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg
      541 ttcacccgga gctcgccgta gcgctcgata tcaccgatga acaaccggtg acttggttta
      601 gcccgttaga tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga
      661 atgaagacgg tacgctggca cgcattgaag agaaatacct ggggcatggc gatgattttg
      721 attacgtcga tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc
      781 ccctgtttga gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc
      841 aggaatcgca ctgggatgca caggccactt caccgacggg tgtgcgcggc atgatgatgt
      901 taaccaaaaa taccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca
      961 tcagcggtgg cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg
     1021 agaacgagcg gatctggttt gccctcgctg cgtacaatat gggctatgcg catatgctgg
     1081 atgcccgcgc cctgacggca aaaaccaaag ggaatcctga cagttgggct gacgtaaaac
     1141 agcgtctgcc tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc
     1201 gtggacatga agcctacgct tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg
     1261 gttatctgca agagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt
     1321 atccggcggt atcgcctacg gagttgggca aagagaaatt tccttttctc tcgtttcttt
     1381 cccagtcgtc atcaaactat ttgacccatt ctccctctct gctgttttcc aggaaaggga
     1441 gtgaagagaa acaaaattaa tccgtcgagg gttgtgcttt tttctgcgct ttaatttcct
     1501 ggcggcgcat gcgaaagaag tcactgagca acgccgcgca ctcatccgcc agtattcctt
     1561 ccgtaatttc cactcggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg
     1621 cagcgccagt tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca
     1681 tcgctccggc acacattaca catggttcaa gcgtgacata caacgtggcg tcgatcagac
     1741 gataattttg catcaccaga ccaccctgcc gcagcgccat gatttctgca tgtgcggtgg
     1801 gatcatggcg accaatcggg cggttccagc cttcgccgat tacccgattg ttatgcacta
     1861 ataccgcgcc gaccggcact tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt
     1921 gacgcatcca gtattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa
     1981 aagcgcggcg cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac
     2041 cgcgcggggt tactcgccaa cgatgctgac agaaataaag cagcggattg tcctgattac
     2101 tgtcgctata gccactgtac agccgcagcg gagtgccgat tttgcgctcc agttgtgcga
     2161 ccttttcatg tcccagacaa cgcatcgtca atacccaacc accatagcca cgctgaattt
     2221 ggctggcgat aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca
     2281 gcggctgcgg agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg
     2341 ttaattgctc ctgaaccagc ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat
     2401 cggcctgcaa cgtctgtaaa cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca
     2461 gactcatcgg ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct ataatcggta
     2521 acaacggcag gacaagtaac gcattcagcg gttggcgacg tagtaaatag cgcagaaaac
     2581 tgccgaacat atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct
     2641 cgtgagttgc caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac
     2701 atagcctaac agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg
     2761 gaatttttaa ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc
     2821 atattgcctg ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta
     2881 tccgtcagcg ttaccagggg cttgcccaga gtgataaaaa actggcggat tatctgctgc
     2941 tacaacctga tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca
     3001 gtcagtccag cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta
     3061 agttggcgtt gagtgaagcg ctggcaagcc agccggaatc gccctccgtg cccattcata
     3121 accaaatccg cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata
     3181 ctgccgctat gtacgcaacg ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa
     3241 caatgttgcg ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg
     3301 cgcaaaactt tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata
     3361 tgcatgcgct actcgcaaca gtacaggcgt cgtcccctga cgatctgtta ttagccattt
     3421 cctacaccgg tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg
     3481 gaaaagtgct ggcgattacc ggctttactc cgaatgccct gcaacagcgt gcttctcatt
     3541 gcctgtatac cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg
     3601 ctcagggaat gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg
     3661 caccagaacg tattcgtcat agtgaagcgc tgatgaaaaa actggtctga gtaaagaatg
     3721 cgcgtataat gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gttgttaatc
     3781 actaaaaaat gcatcaattg tgatatgtgt gaacccgaat gcccgaatga ggcgatttca
     3841 atgggagatc atatctacga gattaacagc gataagtgta ccgaatgcgt agggcactac
     3901 gagacaccaa cctgccagaa ggtgtgcccg atccccaata ctattgtgaa agatccggcg
     3961 catgtcgaga cagaagaaca gttgtgggat aaatttgtgc tgatgcacca cgcggataaa
     4021 atttaatatt ctactctgga agtagagtat taattatatt actgggaagc cttaacgcca
     4081 ttatatttat ttaattgatg acattagcat aatcattcac taagttaatt tatatagtat
     4141 ct
//
LOCUS       DAJSKJ010000239         4072 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000239 DAJSKJ010000000
VERSION     DAJSKJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.243"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            163..771
                     /locus_tag="ON345_003689"
     CDS             163..771
                     /locus_tag="ON345_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR3073672.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            869..2260
                     /gene="selA"
                     /locus_tag="ON345_003690"
     CDS             869..2260
                     /gene="selA"
                     /locus_tag="ON345_003690"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR3073673.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAGFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2257..>4072
                     /gene="selB"
                     /locus_tag="ON345_003691"
     CDS             2257..>4072
                     /gene="selB"
                     /locus_tag="ON345_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR3073674.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLL"
BASE COUNT          911 a         1034 c         1210 g          917 t
ORIGIN      
        1 agccttaagt gagatatcca tcagatagaa acaattagga aaatatttat atcaccatga
       61 taaatatgtg atttatttgg aagtaacaaa ccatcaacat tttctgccct ttttcccatc
      121 accaggccat actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc
      181 tacaccagcc cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc
      241 gaatttatta atgaactgcc ctataacgcg gacaacggcg tggcgcaatt taacccgtta
      301 ggaaaagtgc cggtgctggt gaccgaagag ggcgaatgtt ggtttgattc gccgatcatc
      361 gctgaatata ttgaattaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag
      421 tcgttgcggg tgcgcaaaat tgaggcactg gcggatggca ttatggatgc cgggctggta
      481 tcggtgcgtg aacaggcgcg cccagcggcg cagcagtctg aagatgaatt gttacgccag
      541 cgggagaaaa tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc
      601 aaaaccgata cggtcaatct ggcgactatc gccattgcct gtgccgtcgg atatctcaat
      661 ttccgccgcg ttgcgccagg ttggtgtgtc gaccgcccac acttagtcaa actggtcgaa
      721 aacctgttta gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata
      781 tgtcctcctg acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt
      841 gagagggcct tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca
      901 acttccggct attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta
      961 tggtcacacc cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat
     1021 tcgtggcagc cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg
     1081 gttgacgaaa gaagcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct
     1141 gcataccaac cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc
     1201 tatgcgttcg ccagtgaccc tcgagtacga tctggacgac gccggacgcg gacatcgcga
     1261 tcgggcgctg gcgcagctgc tgtgccgtat tacgggggcg gaagatgcct gtatcgtcaa
     1321 taacaatgcg gcggcggtgt tattgatgtt ggcggccact gccagcggaa aagaggtggt
     1381 ggtatctcgc ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg
     1441 tcaggcaggc tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta
     1501 tcgtcaggcg gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag
     1561 cattcagggg ttcaccaaag cgatagatga agcggaactg gtggcgctcg gcaaagagct
     1621 ggatgttccc gtagtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg
     1681 tttgccgaaa gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagttt
     1741 ctccggcgac aagttgttag gcgggccgca ggcaggaatt attgttggta aaaaagagat
     1801 gatcgcccgc ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct
     1861 cgcggcgctg gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt
     1921 accgaccctg cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt
     1981 acaggctccc cttgccgcac attacggcgc ggggtttgcg gtacaggtta tgccatgtct
     2041 ttcgcagatt ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt
     2101 tacaccccat gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt
     2161 gccagtgccg gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct
     2221 tgaagatgag caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac
     2281 gttgaccacg gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg
     2341 ccggaagaaa aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg
     2401 gatggtcgcg tgcctggttt tatcgacgta cccggtcatg aaaagtttct ttccaacatg
     2461 ctggcgggcg ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggcgtg
     2521 atggcgcaga cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca
     2581 gtggcgctga ccaaagccga tcgcgtggac gaagcgcgtg ttgatgaggt tgaacgccag
     2641 gtaaaggagg tgctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca
     2701 accgaaggtc ggggaattga tgccctgcgc gagcatctgc ttcagttgcc ggaacgcgag
     2761 cacgccagcc aacatagttt ccgcctcgct attgaccgcg catttaccgt aaaaggtgcc
     2821 gggctggtcg tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactctgg
     2881 ctgactggtg taaataaacc gatgcgtgta cgtgcgctgc atgcgcaaaa ccagccaaca
     2941 gaaaccgcca atgccgggca gcgtatcgcg cttaacatcg cgggtgatgc ggaaaaagag
     3001 cagattaacc gtggcgactg gctgcttgcc gatgtgccgc cagagccgtt cacacgggtg
     3061 attgtcgagc ttcaaaccca tacaccgctg acccagtggc agccgctgca tattcaccac
     3121 gccgccagcc acgtcacggg acgcgtttca ctgctggaag ataaccttgc cgagctggtg
     3181 ttcgacacgc cgctgtggct ggcggataac gatcgcttag tgctgcgcga tatctcggcc
     3241 cgcaacacgc tggcgggggc gcgtgtggtg atgcttaacc cgccgcgtcg cggtaaacgt
     3301 aagccggaat atctgcaatg gctggcgtct cttgcacggg cgcagagcga tgccgatgcg
     3361 ttatctgttc atctggaacg cggcgcggtt aaccttgcgg atttcgcctg ggcgcgccag
     3421 ctcaacggcg aagggatgcg cgaattgctg caacagcctg gctatattca ggctggttat
     3481 agcttgttga atgcgccggt tgccgctcgc tggcaacgga aaattctcga cacattagcg
     3541 acttatcatg agcaacatcg cgatgaacct gggcctgggc gcgaacgtct gcgacgtatg
     3601 gcgttgccaa tggaagatga agcgctagta ctgttgctga ttgaaaagat gcgcgaaagc
     3661 ggcgacatcc tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc
     3721 gaagagcagc aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg
     3781 gtgcgtgatc tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc
     3841 caggcggcgc agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat
     3901 cggattgtcg aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc
     3961 gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa
     4021 tattttgacc gcattggctt tacgcgtcgt cgtggaaatg atcatttatt gc
//
LOCUS       DAJSKJ010000240         4058 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000240 DAJSKJ010000000
VERSION     DAJSKJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.244"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            116..3856
                     /gene="rhsB"
                     /locus_tag="ON345_003692"
                     /pseudo
     CDS             116..3856
                     /gene="rhsB"
                     /locus_tag="ON345_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          939 a         1190 c         1241 g          688 t
ORIGIN      
        1 taaatatttt cctaattgtt cttatctgac agatatctca cttaaggctt tcttataaat
       61 ctgtagggtt tcgcctgtca gcagacaaat aacccgataa aacaaggatg agcagatgag
      121 cggaaaaccg gcggcgcgtc agggcgacat gacgcagtat ggcggtagca ttgtccaggg
      181 ttcagccggg gtacgcatcg gtgcccccac cggcgtggcc tgttcggtgt gccccggcgg
      241 ggtgacgtcc ggccatccgg tcaatccgct gctcggtgca aaggtccttc ccggtgaaac
      301 cgacatcgcc ctgcccggcc cgctgccgtt cattctctcc cgcacctaca gcagttaccg
      361 gacaaaaacg cccgcgccgg tggggagcct cggccccggc tggaaaatgc ctgcggatat
      421 ccgcttacag ctgcgcgata acacactgat actcagtgat aacggcggca gaagcctgta
      481 ttttgagcac ctgtttcccg gtgaggacgg ttacagccgc agcgagtcac tgtggctggt
      541 gcgcggcggc gtcctgagac tggatgaagg tcaccggctg gccgcactct ggcaggcgct
      601 gccggaagaa ctccgcttaa gtccgcatcg ttatctggcg acaaacagtc cgcaggggcc
      661 gtggtggctg ctcggctggt gtgagcgggt gccggaagcg gatgaggtgc tgcctgcgcc
      721 gctgccgccg taccgggtac tgaccgggct ggtggaccgc ttcgggcgca cacagacgtt
      781 ccaccgcgaa gccgccggtg aattcagcgg cgaaatcacc ggcgtgacgg atggtgccgg
      841 gcgtcacttc caactggtac tgaccacgca ggcgcagcgg gcagaagaag cccggcagca
      901 ggccatttcc ggcgggacgg aaccgtccgc ttttcctgat accctgccgg gttacaccga
      961 atatggccgg gacaacggca tccgtctgtc tgccgtgtgg ctgacgcacg acccggaata
     1021 cccggagaat ttacctgccg cgccgctggt acgctatggc tggacgccgc gcggcgaact
     1081 ggcggtggtg tatgaccgta gtggcaaaca ggtgcgcagc tttacttacg atgataaata
     1141 ccggggccgg atggtggcgc accgtcacac gggccggccg gaaatccgtt accgttacga
     1201 cagcgacggg cgggtgacag aacagcttaa cccggcaggc ttaagctaca cgtatcagta
     1261 tgagaaagac cacatcacca tcaccgacag cctggaccgc cgtgaagtgc tgcacacgca
     1321 gggcgaagcc gggctgaagc gggtggtgaa aaaggaacac gcggacggca gcgtcacgca
     1381 gagtcagttt gacgcggtgg gcaggcttaa ggcacagaca gatacagcag gccggacaac
     1441 agaatacagc ccggatgtgg tgacgggcct catcacgcgc atcaccacgc cggatggcag
     1501 ggcatcggcg ttttactata accaccacaa ccagttaacg tcagccaccg ggcctgacgg
     1561 gctggaattg cgccgggaat atgatgaatc cggccgtctg attcaggaaa ctgcccctga
     1621 cggcgatatc acccgctacc gttatgataa tccacacagt gacttaccct gcgcaacgga
     1681 agatgccacc ggcagccgga aaaccatgac gtggagccgt tacggccagt tgctgagctt
     1741 caccgactgt tccggttatg taacccgtta tgaccatgac cgttttggtc aggtgacggc
     1801 ggtgcaccgc gaggaaggcc tgagccagta ccgcgcatac gacagccgtg gacagttaat
     1861 tgccgtgaaa gacacgcagg gccatgaaac gcggtatgaa tacaacgccg ccggtgacct
     1921 gaccaccgtc attgccccgg acggcagcag aaacgggaca cagtacgatg cgtggggaaa
     1981 ggccatctgt accacgcagg gcgggctgac gcgcagtatg gaatacgatg ctgccggacg
     2041 ggtcatccgc ctgaccagtg aaaacggcag ccacaccacc ttccgttacg atgtactcga
     2101 ccggctgata caggaaaccg gctttgacgg tcgcacacag cgttatcacc acgacctgac
     2161 cggtaaactt acccgcagcg aggatgaggg gctggtcacc cactggcact atgacgaagc
     2221 agaccgcctc acgcaccgca ccgtgaatgg cgaaaccgca gagcagtggc agtatgacga
     2281 acgtggctgg ctgacagaca tcagccatat cagcgaaggg caccgggtgg cggtgtatta
     2341 cggatctgat gagaaaggcc gcctgaccgg tgagcgccag acggtgcatc acccggagac
     2401 ggaagcactg ctctggcagc atgagaccag acatgcgtac aacgcgcagg gactggcgaa
     2461 ccgctgtata ccggacagcc tgcccgccgt ggaatggctg gcctacggca gcggttacct
     2521 ggcaggcatg aagctcggcg acacaccgct ggtggatttc acccgcgacc gcctgcaccg
     2581 ggaaacgctg cgcagcttcg gtcgttatga actcaccacc gcttacaccc ctgccgggca
     2641 gttacagcgt cagcacctga acagcctgca gtatgaccgc gattacacct ggaacgacaa
     2701 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
     2761 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
     2821 cacagacccg gcaggtaacc gcctgcccga cccggagctg cacccggaca gcgccctcag
     2881 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccgtca
     2941 cggcaggctg acagagaaaa ccgacctcat cccggaaggg gttatccgca cggatgatga
     3001 gcgcacccac cggtaccatt acgacagtca gcaccggctg gtgcactaca cgcggacaca
     3061 atatgcagag ccgctggtcg aaagtcgcta tctttacgac ccgctgggcc gcagggtggc
     3121 aaaacgggta tggcggcgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc
     3181 gcaagtgacc tggtacggct gggacggcga ccgcctgacc acgatacaga acgacagaac
     3241 ccgcatccag acgatttatc agccggggag cttcacgcca ctcatcaggg ttgaaaccgc
     3301 caccggtgag caggcgaaaa cgcagcgccg cagcctggcg gatacccttc agcagtccgg
     3361 cggcgaagac ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct
     3421 ggaaagtgaa atcctggctg accgggtgag tgaggaaagc cgccgctggc tggcatcgtg
     3481 cggcctgacg gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa
     3541 aatccacctg taccactgcg accatcgcgg cctgccgctg gcccttatca gcaaggaagg
     3601 gacaacagaa tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc
     3661 gcatcagctg cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct
     3721 gtattacaac cgccaccgct attatgaccc gctgcagggg cgatatatca ctcaggatcc
     3781 gattgggctg aaagggggat ggaattttta tcagtatccg ttgaatccga tctcaaatat
     3841 agatccatta ggattagaaa cactaaaatg cattaagcca ctgcattcaa tgggcggaac
     3901 tggtgaaaga agcggtccag atatatgggg gaatccgttc tatcatcaat atctttgtgt
     3961 cccagatggt aaaggggact atacttgtgg tggccaagac caacggggag aatcaaaagg
     4021 agatggtcta tgggggccag gtaaagcaag taatgata
//
LOCUS       DAJSKJ010000241         3883 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000241 DAJSKJ010000000
VERSION     DAJSKJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.245"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..983
                     /locus_tag="ON345_003693"
     CDS             <1..983
                     /locus_tag="ON345_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3073675.1"
                     /translation="NLLSVKNYGEFEFWLALCKVIAILAFIFLGAVAISGFYPYAEVS
                     GISRLWESGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRATNSVI
                     WRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSC
                     LNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAK
                     VFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITF
                     VLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            1004..1666
                     /gene="csiR"
                     /locus_tag="ON345_003694"
     CDS             1004..1666
                     /gene="csiR"
                     /locus_tag="ON345_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR3073676.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKVHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(1667..2116)
                     /gene="kbp"
                     /locus_tag="ON345_003695"
     CDS             complement(1667..2116)
                     /gene="kbp"
                     /locus_tag="ON345_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR3073677.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(2200..2358)
                     /locus_tag="ON345_003696"
     CDS             complement(2200..2358)
                     /locus_tag="ON345_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR3073678.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2541..2840
                     /locus_tag="ON345_003697"
     CDS             2541..2840
                     /locus_tag="ON345_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3073679.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2850..3374
                     /gene="ygaP"
                     /locus_tag="ON345_003698"
     CDS             2850..3374
                     /gene="ygaP"
                     /locus_tag="ON345_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR3073680.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(3421..3825)
                     /gene="stpA"
                     /locus_tag="ON345_003699"
     CDS             complement(3421..3825)
                     /gene="stpA"
                     /locus_tag="ON345_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR3073681.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          930 a          930 c          972 g         1051 t
ORIGIN      
        1 gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgcaaag
       61 tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt ttttaccctt
      121 atgccgaagt gagcgggatc tcaagattgt gggaaagcgg cggctttatg cccaacggct
      181 tcggcgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg
      241 tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc gccaccaact
      301 cggttatctg gcgtatttct atcttctatt tatgttctat ttttgtcgtc gtggcattaa
      361 ttccgtggaa tatgcccgga ctaaaagccg tcggttctta tcgctcggta ctggaattgc
      421 tcaatattcc ccatgcgaaa ttgatcatgg actgcgtgat attactttcc gtaaccagtt
      481 gcctgaactc ggctctgtat accgcttcca ggatgctcta ctccttaagc cgtcgcggag
      541 atgcgcctgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac
      601 tctccaccgg cgcggcattc ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtat
      661 ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg
      721 tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcttgcgca
      781 tgtggcttta tccgtggctc acctggctgg tcatcggctt tattaccttt gtgttggtag
      841 tgatgctatt ccgtccggcg caacagttag aagtgatctc caccggctta ttagcgatag
      901 ggattatctg taccgtgccg attatggcgc gctggaaaaa gctggtattg tggcaaaaaa
      961 cacccgttca taatacgcgc tgataatgat caggagtcac accatgacca ttacgtctct
     1021 ggatggctat cgctggctga agaacgatat tattcgcggt aattttcaac cggatgaaaa
     1081 attacgaatg agtttgctga catcgcgtta tgcacttggc gtcggaccgt tgcgggaagc
     1141 actttcgcaa ctggtggcag aacggctggt cacggtggtg aatcaaaaag ggtatcgggt
     1201 ggcgtcaatg tcagagcagg agctgctcga tattttcgac gcccgcgcca atatggaagc
     1261 gatgttggtg agtctggcga ttgcccgcgg tggcgatgag tgggaggcag acgttctcgc
     1321 aaaagtgcat ctgctcagta aactggaagc ctgtgacgcc agcgagaaaa tgcttgatga
     1381 gtgggatctg cgtcatcagg cgtttcatac ggcaattgtg gcgggctgtg gttctcacta
     1441 tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgctatcgat ttatctggct
     1501 gcggcgaaca gtgctttcgg tggaaatgct ggaggataaa cacgatcagc accagaccct
     1561 gactgcggcg gtactggcgc gagataccgc gcgcgccagt gagttaatgc gccagcattt
     1621 actgacgcca attcccatta tccagcaggc gatggctggc aattaactac tcttcaggaa
     1681 tacgcaacac ttgccccggg taaattttat ccgggctttt tagcatcggt ttattcgctt
     1741 cgaagatttt attgtacaga ttggcgttac cgtagacctg tttggaaatg gcactcagag
     1801 tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgct gttttcacct
     1861 gatcatcgac actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg
     1921 cctcctgact caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatattca
     1981 ctttatcggc atccggtata ccggttttgt tcagatgctc ctgcaccttc ttcgcctgat
     2041 cgtctttatc gtgctgacct gtaaccgcgt cccagagttt ttctccggca tctttcacaa
     2101 aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga
     2161 ggaacgcggc gcgttaacgc cgcggggatg agcacaggtt taatcgcggg tttgcaccca
     2221 gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc aacagaatat taataatgaa
     2281 cgcccaaccg aaccctttac cgagcagcac gccgagaggc ggcagaatga tggtgatgac
     2341 gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga
     2401 ttttatctct taagcgtagt aattttagcg gagcctgcca gtttcagctt acgcttttac
     2461 tctcatttac gctgctgatg ctggtatatg ttgcattttt agtttagagt ttacttaatt
     2521 tagaaaatac ttaaataact atgactgaac tcgcgcaatt acaggccagt gccgaacagg
     2581 cagcggcctt attgaaagct atgagccacc ctaaacggtt gctgattctg tgcatgctta
     2641 gcggttcccc cggcaccagc gcgggagagc tgacgcgcat taccggactg agtgcctctg
     2701 cgacatcaca gcatcttgct cgtatgcggg acgaagggct tatcgacagc caacgggatg
     2761 cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc gccaccctga
     2821 aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg ccgcatgatg
     2881 cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgatgct gatgaatatc
     2941 ttcgtgaaca tattcctgaa gcagatctgg ctccattatc cgtgctggaa cagtcaggtc
     3001 ttccgcctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt aaacgcacca
     3061 gtaataacgc cgataaatta gcagcgattg ccgcccccgc agaaatattt ttactcgaag
     3121 atgggattga tggctggaaa agagctggat tgccagtagc ggtaaataaa tctcaaccct
     3181 tgccgttaat gcgccaggtg cagatcgctg cgggtggttt aatattaatc ggcgttatac
     3241 tgggttatac cgtaaatagc ggtttcttct tattaagtgg ctttgttggt gccgggttac
     3301 tgtttgcagg aatcagcggt ttttgtggaa tggcaaggtt gttagataag atgccgtgga
     3361 accaacgagc ttgagaagcg acgccggacg cgccctagca gcgacatccg gcctcagtaa
     3421 ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcaa ttggcttcgg
     3481 tgtacggccc tgaccggtcc aggttttagt ttcaccgtta acatcggtga atttatattt
     3541 cgccggacgc ggctggcgtt ttttaccagc gcgtggtgca gcagcagagc tattacctaa
     3601 taactcttcc gggttaattc cgtcagcctt catcagctcc agccaggtgc taattttttc
     3661 ctgacgctca gccagttcac gctgctgctg ttcttcttct tcacgtcttt ctttagtgac
     3721 aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc gagccatcgc
     3781 acggagagtg cgaatgttat ttaaactttg taacattacg gacataataa agaaaacctt
     3841 ttaacgccaa aacaaaaaag tatttcgtcg cgtagtgtaa gcg
//
LOCUS       DAJSKJ010000242         3795 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000242 DAJSKJ010000000
VERSION     DAJSKJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.246"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(123..869)
                     /gene="cutC"
                     /locus_tag="ON345_003700"
     CDS             complement(123..869)
                     /gene="cutC"
                     /locus_tag="ON345_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR3073682.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(883..1449)
                     /locus_tag="ON345_003701"
     CDS             complement(883..1449)
                     /locus_tag="ON345_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3073683.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1665..3398
                     /gene="argS"
                     /locus_tag="ON345_003702"
     CDS             1665..3398
                     /gene="argS"
                     /locus_tag="ON345_003702"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR3073684.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALAS
                     DRLDVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3575..>3795
                     /locus_tag="ON345_003703"
     CDS             3575..>3795
                     /locus_tag="ON345_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR3073685.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYR"
BASE COUNT          982 a          953 c          952 g          908 t
ORIGIN      
        1 aacagatcac ggttatcgtt cgttttttat actgctcagg gataaaaaaa ggcccctgtt
       61 gaaattgcag gggcctggta cgagcaagca tcatattggg cgacatgatg caacggtaaa
      121 aatcatttgg cctgatggcg ttcaatgatt cctttcattt cagcaaccgc cgccccgtct
      181 acgatataac gcgaatactc gtccgcgtgt tcatctgatg acatggacaa tccttgatta
      241 cgataacgca tcggtgaggc ttgccacgct cccgcggagc tatggacttc cagcactccg
      301 gcatcgagga agtggtgcag gttttctgca cggactcctg ctccggccat aatgattgga
      361 gcatcacgat gggcaataag ttccataatt tttgataaac cttgcagtgc gtctgatttt
      421 tgccctgatg tcagtacccg ggcaatgccc aattccgcaa gattattgag tgtatataaa
      481 gggttagcgc acatatcgaa ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc
      541 attatttttt ccattcgtgg catatcgaca ttcccgtcaa catcgagaac gcccgtcacc
      601 agtccaggaa aacctaattc gcggaccgtg cgcacatcct caagaatggc ggcaaactca
      661 ccgtcgctgt agcaaaaatc accaccgcgt gggcgaatta tcggatgcac agggatcgtc
      721 acccgctggc gcacggattt cagtaccccc aacgacggcg ttaagccccc ctcttttggg
      781 gctgcgcata attcaactct gtctgcgccg ttttgctgcg ccgttagtgc acattccatg
      841 ctgtaacagc aaatttccag taatgccatt tttgctcctt aattacgctg actgctcgct
      901 ggcaacgatc tcttcaatgg accacggatg aaacttaata gttgttttgc catcggtgac
      961 cgccagcgtc ggattcggta atcgctcatg ctcacctttt ggtgaactag ttttaactga
     1021 gatccccggt aggctgagtc cttcatcaga aagcaatgcc agcgcacggg cattcagcgt
     1081 ttctggatca cccggcagaa cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt
     1141 ttccccgggc cacggtagct ccacaataga aaactgccag tgcgcaacct gtaccggttc
     1201 atgcaattta aacagacaaa tcggtctgcc attgatcata ttttctgaca aaagctcgcc
     1261 acactgttca aacccgcgac gccagcgctc agcggtgacg ttttgatggc aacgcaaaga
     1321 aatgtgatcg gcagtcagcg gagtgatatt caaccccaga cggcgggaaa gttcatctaa
     1381 tgcgtgtgta aatcgcggta aatccgatgc aatatcctgc agctcgtcga tagattgcca
     1441 gttcgccata atcactcttc gtctttcagt aaaagcgtta atttaccctg ttgccctgtg
     1501 ccaaccaacc gctgatttca cgccgcttct gatgcaatag tgaaaacggc aatacgccac
     1561 gcgcacgttg ctgacgaaaa cagccatttg cagtatactc cctccctaat ttctttaact
     1621 ggtgcgggca atttttgctc gcttcatcaa tgtaaggtat tccggtgaat attcaggctc
     1681 ttctctcaga aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg gattgcgaac
     1741 cgcaggttcg tcagtcagca aaagttcagt tcggcgacta tcaggctaac ggcatgatgg
     1801 cagttgctaa aaaactgggt atggcaccgc gacaattagc agagcaggtg ctgactcatc
     1861 tggatcttaa cggtatcgcc agcaaagttg agatcgccgg tccaggcttt atcaacattt
     1921 tccttgatcc ggcattcctg gctgatcatg ttcagcaggc gctggcgtcc gatcgtctcg
     1981 atgttgctac gccagaaaaa cagaccattg tggttgacta ctctgcgcca aacgtggcga
     2041 aagagatgca cgtcggtcac ctgcgctcta ccattattgg tgacgcagca gtgcgtactc
     2101 tggagttcct cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg ggcactcagt
     2161 tcggtatgct gattgcatgg ctggaaaagc agcagcagga aaacgccggt gaaatggagc
     2221 tggctgacct tgaaggtttc taccgcgatg cgaaaaagca ttacgacgaa gatgaagagt
     2281 ttgccgagcg tgcacgtaac tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg
     2341 agatgtggcg caaactggtc gacataacca tgacgcagaa ccagatcacc tacgatcgtc
     2401 tcaacgtgac gctgacccgt gatgacgtga tgggcgaaag cctctacaac ccaatgctgc
     2461 caggaattgt ggcggatctc aaagccaaag gtctggcagt agaaagcgaa ggggcgaccg
     2521 tcgtattcct tgatgagttt aaaaacaagg aaggcgaacc gatgggcgtg atcattcaga
     2581 agaaagatgg cggctatctc tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg
     2641 aaacactgca tgccgatcgc gtgctgtatt acatcgactc ccgtcagcat caacacctga
     2701 tgcaggcatg ggcgatcgtc cgtaaagcag gctatgtacc ggaatccgta ccgctggaac
     2761 accacatgtt cggcatgatg ctgggtaaag acggcaaacc gttcaaaacc cgcgcgggtg
     2821 gtacagtgaa actggccgat ctgctggatg aagccctgga acgtgcacgc cgtctggtgg
     2881 cagaaaagaa cccggatatg ccagctgacg agctggaaaa actggctaac gcggttggta
     2941 ttggtgcggt gaaatatgcg gatctctcca aaaaccgcac cacggactac atcttcgact
     3001 gggacaacat gctggcgttt gaaggtaata ccgcgccata catgcagtat gcttacacgc
     3061 gtgtattgtc cgtgttccgt aaggcagaaa ttaacgaaga gcaactggct gcagctccgg
     3121 taatcatccg tgaagatcgt gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa
     3181 ccctcaccgt ggttgcccgt gaaggcacgc cgcatgtgat gtgtgcttac ctgtacgatc
     3241 tggctggtct gttctctggc ttctacgagc actgcccgat cctcagcgca gaaaacgaag
     3301 aagtgcgtaa cagccgtctg aaactggcac aactgacagc gaagacgcta aagctgggtc
     3361 tggatacgct gggtattgag acagtagagc gtatgtaatc gatttttcgt gagagtaaag
     3421 cctgatcagg gttacctgat caggcttttt tattgccatc taaatgtatt ctgaaaatgg
     3481 acatgccatt gttttctgac tgttggataa gaggccagaa gcgtaatatc cggccccagg
     3541 gaaacgataa cggttgaatt taaggaatac cgcagtgttt aaatttcttg tattaacatt
     3601 aggcattatc tcttgccagg cttacgcaga agatacggtt atagtaaacg accatgacat
     3661 ttcagccatc aaagattgtt ggcaaaaaaa ttcagatgat gatactgaca ttaacgtgat
     3721 caaatcatgc ctgcgacaag aatacaatct cgtcgatgcg caattaaata aagcctatgg
     3781 tgaagcttat cgtta
//
LOCUS       DAJSKJ010000243         3709 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000243 DAJSKJ010000000
VERSION     DAJSKJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.247"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..290
                     /locus_tag="ON345_003704"
     CDS             <1..290
                     /locus_tag="ON345_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR3073686.1"
                     /translation="ELGATLSYNMRLGNGMEIEPCLKAAVRKEFVDDNRVKVNSDGNF
                     VNDLSGRRGIYQAGIKASFSSTLSGHFGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(662..928)
                     /gene="minE"
                     /locus_tag="ON345_003705"
     CDS             complement(662..928)
                     /gene="minE"
                     /locus_tag="ON345_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR3073687.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(932..1744)
                     /gene="minD"
                     /locus_tag="ON345_003706"
     CDS             complement(932..1744)
                     /gene="minD"
                     /locus_tag="ON345_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR3073688.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1768..2463)
                     /gene="minC"
                     /locus_tag="ON345_003707"
     CDS             complement(1768..2463)
                     /gene="minC"
                     /locus_tag="ON345_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR3073689.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            2983..3351
                     /locus_tag="ON345_003708"
     CDS             2983..3351
                     /locus_tag="ON345_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073690.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            complement(3471..>3709)
                     /locus_tag="ON345_003709"
     CDS             complement(3471..>3709)
                     /locus_tag="ON345_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR3073691.1"
                     /translation="AKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQY
                     SLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
BASE COUNT          935 a          835 c          886 g         1053 t
ORIGIN      
        1 gtgaactggg tgcaacgctg agttacaaca tgcgtctggg gaacggtatg gaaattgaac
       61 cctgcctgaa ggcggctgtg cgcaaagaat ttgtcgatga taatcgggtg aaagtgaata
      121 gtgacggtaa ttttgtcaat gatttgtcgg gcagacgtgg aatataccag gcaggtatta
      181 aagcctcatt cagcagtacg ttaagcgggc atttcggggt ggggtatagc catggtgccg
      241 gtgtggagtc cccgtggaac gcggtggctg gtgtgaactg gtcgttctga ccatcaacga
      301 ttaaactgcg cttcggcgca gttttcgttt acaggatgtt gaaagagaaa attctggggc
      361 aaaaaaagcc cgccagttac ggcgggaaac ctcatcctat gggggaacaa tgaataatga
      421 aattgcgggg ttatcatctc ccagtatatc catactaaca acatggttat ttactcaacc
      481 aggcataaac attttgttct gtgcgtggga acagccttaa ggtgtaaagg gggaggtgga
      541 aatagcaatg aggagtatca gcaagaatac tcgccgcttt accacaacgt ggatgagagg
      601 gatgaaaaac tcaaggcaga aatgactctg ccttgaagat aaatgcgttt ttacagcggg
      661 cttatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc
      721 catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa
      781 tcacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca tcgctgcgac
      841 ggcgttcagc aacaataatc tgcagccgtt cttttgcaat gttggctgtg tttttcttcc
      901 gcgagagaaa gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc
      961 ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg
     1021 tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt
     1081 tagaggcgcg caatactgat tgatcctctg ggatcacgcc gacgagtttg atgcgcagga
     1141 tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc
     1201 gcgttaacag caggtgctct ttaataggct cttcgccatt ttctgcgcgg cgtgatttcg
     1261 acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg
     1321 taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca atccctgccg
     1381 gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga actttggcga
     1441 ccccttcacg ggtgagggca tctttatcgc gtgtttgcga tgccggaaga atatagagat
     1501 tttcagtacg cttatcttta attaacgcct gatttagcgt tgcatcgccc tgaatgacgt
     1561 tgacgaaatc gtaaacgacc cggcgttcac aacccataat caggtcgaga ttacgcaggc
     1621 cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg
     1681 ccgcgctgga ggttgtctta ccaacacccc ctttgcccga agtaacaaca ataatgcgtg
     1741 ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc
     1801 gactaactgc agtcgcgccg ctttgccata aaattctgct gggatttgat cactcagcca
     1861 gtattcacct gcgatggaca ccagttccgc catcaggttc gtacaaaata tttgcgtttc
     1921 ccggtcacca cttgcccctg caagcgcacg accgcgcatc atgccataga catgaatgtt
     1981 cccatcggca atcaattcgg ccccagcgct aacgtggctt gtaacaatca gatcacattg
     2041 tggagcataa atacgctgac cggaacgcac cggggtatct attaaacgcg tttttgtgac
     2101 cggcgttgta ttttgcgctg gagcctgtgg tgcgggagct ggacgcggcg ctttttcctt
     2161 tccttccgtc aggataggca gccccatctt ttcaatttcg gctttaagtt gcgcatcttt
     2221 gcagccactt acgccaataa cccgcaaacc ggttgccgaa accgccttat gcatcgctga
     2281 ccagtttacc gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa
     2341 tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc
     2401 atgcagatga accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga
     2461 catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga
     2521 ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga
     2581 gtaaaagtct attctgtgat aaatggcgct gattcatagc ttaaaaaata cccttgtcaa
     2641 tcaacccatt gccgccgtac ttttgattct tcttatttac gcttcctttt tccgcatcct
     2701 aatcgttaca gcatctccag acctgtttaa atatccagta ttgaggcaag tgtatttttt
     2761 caccacgcca ggctttctta actttccatt ttttgcagca tcattaaaga acagataccc
     2821 gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag acgtctgata
     2881 cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgatga
     2941 aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt
     3001 ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc tggacatgat
     3061 gctggcggac ttcaatctcc ggcgtgcggt gttgtttgcg acccgtatat ttgtgtgaac
     3121 tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac
     3181 ttacaatcat tacaaggcta cgatcccagc gaatttacat tcgctaacgg tgtattttgc
     3241 gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtggatgg taagcggagt
     3301 ggaaaaatca atcaaatcac cacaaagatg ttatttatgt gtcgtgagtg atgccttgtt
     3361 tacattatgt tctattcggc attgtctgct attcgtcatt aacctcttga gcgacagaga
     3421 ggagtggggc gttaacgttt atgacgggga gagtccccgt cggttgacat ttacttaatt
     3481 tgaatatcga cgttatattt tttagttttg tttttacggg cgtcgtttcg ggtttgcagc
     3541 actcgcagct catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct
     3601 ggggaatatc tggataaatc aacagaatca tcgattcctg gcccgaaaag gtaagtatca
     3661 gcgccttcat tagagatact cacatgtact ttctgacctt ttttggcat
//
LOCUS       DAJSKJ010000244         3707 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000244 DAJSKJ010000000
VERSION     DAJSKJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.248"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            32..997
                     /gene="lipA"
                     /locus_tag="ON345_003710"
     CDS             32..997
                     /gene="lipA"
                     /locus_tag="ON345_003710"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR3073692.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1098..1301)
                     /gene="tatE"
                     /locus_tag="ON345_003711"
     CDS             complement(1098..1301)
                     /gene="tatE"
                     /locus_tag="ON345_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR3073693.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(1430..2218)
                     /locus_tag="ON345_003712"
     CDS             complement(1430..2218)
                     /locus_tag="ON345_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR3073694.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASDMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2311..2694
                     /gene="crcB"
                     /locus_tag="ON345_003713"
     CDS             2311..2694
                     /gene="crcB"
                     /locus_tag="ON345_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR3073695.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(2749..2958)
                     /gene="cspE"
                     /locus_tag="ON345_003714"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(2749..2958)
                     /gene="cspE"
                     /locus_tag="ON345_003714"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR3073696.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3133..3693)
                     /gene="pagP"
                     /locus_tag="ON345_003715"
     CDS             complement(3133..3693)
                     /gene="pagP"
                     /locus_tag="ON345_003715"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR3073697.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          960 a          950 c          866 g          931 t
ORIGIN      
        1 cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa cccattgtga tggaacgcgg
       61 tgttaaatac cgcgatgccg ataagatggc ccttatcccg gttaaaaacg tggcaacaga
      121 gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc aagcttccag cggactctac
      181 acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga
      241 agcctcctgc cctaacctgg cggaatgctt caaccacggc acagcaacgt ttatgatcct
      301 cggcgctatt tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc
      361 tcctgatgcc aatgaaccag tgaaactggc gcagaccatt gccgatatgg cgctgcgtta
      421 tgtggttatc acctccgttg accgtgatga cctgcgcgat ggcggtgccc agcactttgc
      481 ggattgcatt actgccattc gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc
      541 ggatttccgt ggtcgtatgg atcgtgctct ggatattctg actgcaacgc caccagatgt
      601 gttcaaccat aacctggaaa acgtaccgcg tatttaccgt caggtacggc ctggtgcaga
      661 ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac
      721 caagtctggt ctgatggtgg gactgggtga aaccaatgaa gaaattattg aggtaatgcg
      781 cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg caatatttgc agccaagccg
      841 ccatcacctg ccggttcaac gttacgttag cccggatgag ttcgacgaaa tgaaagccga
      901 agcgctggcg atgggcttta cccatgctgc atgcggtccg tttgtccgct cttcttacca
      961 cgccgatttg caggcgaaag ggatggaagt taagtaatcc tgtaataatt acttacatct
     1021 gtctgataaa aaacccgctt tttgaagcgg gttttttgta tcgaacaaga tattgaggga
     1081 gcgtcctgct cgccacgtca ctctttatga gagagctttt cagcctgaag atcaacgtct
     1141 gcgccttttt tcgccgcagc atcatcatca ttcatcgcct tcttgaaccc tttaatggcc
     1201 gctccaaggt ctccgcccag cgtacgtaac ttcttagtcc caaacagcag aacgaccagc
     1261 gccgcaacta ccagcagttt ggtaatacta atctcaccca tagatacctt cttgacatat
     1321 aaccggcaac agaacgcgcc ttaaaaacca gagaatatta acggtcattc gacgcgcgca
     1381 cacaatagca atctgtaaca aggtgaatca agcgccgagt aaaaaaacat cataataatt
     1441 gcggcggcgc aaagcgacgg ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac
     1501 gttcgggcgt cacttccgcc ataatgagtg caggcatatc tgacgctgcc gcaatggtga
     1561 cgccaaaggg atctataatc cggctttgcc cgatattttt gttcccgcac tcccccgccg
     1621 ccaccatata acaggtggta tccagcgcac gagcggcaag caacgttgac cagtgatgct
     1681 ctttgagcgg cccgcgaacc caggcggcag gaagtaccag gatttcagct ccctgtaatg
     1741 cctgtgccag cgccagctct ggaaagcgta agtcataaca ggtcatcaga ccgaccttca
     1801 tcccttccac ctccagtaac ggagcgattt cattaccagc atcaacacgg cgtgattcct
     1861 gaatggcaaa tgcatcatag agatgcagtt tggcataacg ggcgacgatg tttcctgcct
     1921 gaagtgccac cagcatattc catgcgcgcc ccggcgttga aggaacatga atcgtcagaa
     1981 ttgtcgtcat catgttacgt ttactttctc gccgtaaacg tccgaggaat tcgccttcca
     2041 gcagctgtgc tgatttaacc gatagatctg catcatgatc atcgcgcgcc agcaatgctt
     2101 ccggcaggac aaacagcgat acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa
     2161 tctcagcgtt cttttcccac acagatgtaa cagcaaactg tcctgcggca accagcattg
     2221 ccacctcctg tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt acacttatca
     2281 ctcatacaaa tcaaatagca ggattttgca gtgttacaac ttcttttagc agtttttatt
     2341 ggcggtggta cgggaagcgt ggcgagatgg ctgttaagta tgcgatttaa cccactgcat
     2401 caggcgattc cgttggggac gctgacagca aacctgattg gggcattcat cataggaatg
     2461 ggattcgcat ggttcagcag gatgacgaac attgatccag tgtggaaagt attaatcacc
     2521 accggatttt gtggcggtct aacaaccttc tcaacatttt cggcagaagt ggtgtttttg
     2581 ttacaagagg gtcgctttgg ctgggcatta ctgaacgttt tcgtcaacct tctggggtct
     2641 tttgccatga cagcactggc tttctggctg ttttcggcct caaccgcaca ctaaagacca
     2701 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
     2761 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
     2821 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
     2881 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
     2941 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
     3001 ggttaccagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
     3061 aaagtcgcgt tatttttgat gttacttatt ttagctattg attttaaaga agttactaaa
     3121 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
     3181 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
     3241 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
     3301 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
     3361 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
     3421 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
     3481 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
     3541 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
     3601 catccactca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
     3661 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaa
//
LOCUS       DAJSKJ010000245         3693 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000245 DAJSKJ010000000
VERSION     DAJSKJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.249"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..837
                     /gene="yibB"
                     /locus_tag="ON345_003716"
     CDS             <1..837
                     /gene="yibB"
                     /locus_tag="ON345_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR3073698.1"
                     /translation="ITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMI
                     IFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKN
                     PEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFD
                     ESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKIT
                     LNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLS
                     RK"
     gene            1112..2308
                     /gene="kbl"
                     /locus_tag="ON345_003717"
     CDS             1112..2308
                     /gene="kbl"
                     /locus_tag="ON345_003717"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR3073699.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKGERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITCAVEAFTRIGK
                     QLGVIA"
     gene            2318..3343
                     /gene="tdh"
                     /locus_tag="ON345_003718"
     CDS             2318..3343
                     /gene="tdh"
                     /locus_tag="ON345_003718"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR3073700.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            3582..>3693
                     /locus_tag="ON345_003719"
     CDS             3582..>3693
                     /locus_tag="ON345_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3073701.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALE"
BASE COUNT          941 a          788 c          953 g         1011 t
ORIGIN      
        1 atcactgcat attttgacat tggtagaggg gattggactg ctaataaagg gttccgtgaa
       61 aaactagctc gttcagttga tgtttatttt agttactttg agcgtctggc cgcacttgaa
      121 aatgagatga ttattttcac ttctcccgat ttgaaaccaa gggttgaagc gattcgcaac
      181 ggaaaaccaa caacggttat cgttatcgat ataaaaaaga aatttagata tatcagaagt
      241 cgtatcgaaa aaattcaaaa agatgagtcg tttacaaata gacttgagcc tcgacaatta
      301 aaaaacccag agtactggtc accagagtat gttttggtat gtaaccttaa ggcctatttt
      361 gtaaataaag ctatcaacat gggcttagtg aaaacgcctc ttgttgcgtg gatagatttt
      421 ggctattgcc gtaagccaaa tgtaactcga ggattgaaaa tttgggattt cccatttgat
      481 gaaagtaaga tgcatctttt cactataaaa aaagggctta ctgtcacatc tcaacaacag
      541 gtttttgatt tcatgattgg caatcatgta tatattattg ggggcgcaat tgttggttca
      601 cagcataaat ggaaagaatt ctataaatta gttctggaaa gtcaaaaaat aacactaaat
      661 aataacattg tggatgatga ccagggcatt tttgtcatgt gctattacaa acgccccgat
      721 ctcttcaacc taaactatct ggggagagga aaatggttcg atttgtttcg ctgcttcagg
      781 agtaacactt taggggcaaa aatgcaggcg ctgagaattt tcctttcaag aaaataggtt
      841 tccctccatt agtgggtagc gttttgatac caactgagat aggaaaaatg atgctttggc
      901 atcatttttt ctttttatat caatgcagta aaatcaatca tcattatgcc cagccaaaac
      961 tgtacagttt ttctactatc tgtaggtgtt gagagacatc aagttacacg ttatttatcc
     1021 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac gtataagttt gggtaatatg
     1081 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
     1141 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa ggagagcgca ttattacgtc
     1201 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
     1261 caactatctc gggctggcga atcatccgga tctgattgcg gcagcaaagg cgggaatgga
     1321 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
     1381 agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc
     1441 ctgctttgat gctaacggtg gactgtttga aacgctgctg ggtgcggaag acgccattat
     1501 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgca aagctaaacg
     1561 ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga
     1621 agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcaatgg acggcgtgat
     1681 tgccaacctg aaaggcgttt gcgatctggc agataaatac gatgccctgg tgatggtgga
     1741 tgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
     1801 tgtgatgggc cgggtcgata ttatcaccgg tacgcttggt aaagcgctgg gcggggcttc
     1861 tggtggttat accgcggcgc gcaaagaggt ggttgagtgg ctgcgccagc gttctcgtcc
     1921 gtacctgttc tccaactcgc tggcaccggc cattgttgcc gcgtccatca aagtactgga
     1981 gatggtcgaa gcgggcagcg aactgcgtga ccgtctgtgg gcgaacgcgc gccagttccg
     2041 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcatgcca ttattccggt
     2101 catgcttggc gatgcagtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
     2161 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
     2221 gatgtctgcg gcgcataccc ctgagcaaat tacgtgtgca gtagaagcat tcacgcgtat
     2281 tggtaaacaa ctgggcgtta tcgcctgagg atgtgagatg aaagcgttat ccaaactgaa
     2341 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
     2401 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
     2461 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgta ggccatgaat atgtcggtga
     2521 agtggtaggt attggtcagg aagtgaaagg cttcaaaatc ggcgatcgcg tttctggcga
     2581 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
     2641 caacaccatt ggtgtcggtg ttaaccgtcc gggctgcttc gccgaatatc tggtgattcc
     2701 ggcgttcaac gctttcaaaa tccccgacaa tatctccgac gacctggctt ccatttttga
     2761 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggtgggcg aagatgtgct
     2821 ggtttctggt gcaggtccga taggtattat ggctgcggcg gtggcgaaac acgttggtgc
     2881 acgcaatgtg gtgatcaccg atgttaacga ataccgcctt gagctggcgc gcaaaatggg
     2941 tatcacccgt gcggttaacg tcgcgaaaga aaaccttaat gatgtgatgg ctgaactggg
     3001 catgaccgaa ggctttgatg tcggtctgga aatgtccggc gcgccgccag cgtttcgtac
     3061 catgctcgac accatgaacc acggcggtcg tattgcgatg ctgggtattc caccgtctga
     3121 catgtctatc gactggacca aagtgatctt taaaggcttg ttcattaaag gcatttacgg
     3181 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtctg gcctcgatct
     3241 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
     3301 gtgttcgggc cagtccggga aagttattct gagctgggat taacacgaaa aagggctggt
     3361 attccagccc ttttgcctga ggataatctg ttaaatatgt aaaatcctgt cagtgtaata
     3421 aagagttcgt aattgcgctg atcttttata tagctgttct cattatctct ctaccctgaa
     3481 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
     3541 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
     3601 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
     3661 ctttaattac gcaaacctgg actgctctgg aaa
//
LOCUS       DAJSKJ010000246         3681 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000246 DAJSKJ010000000
VERSION     DAJSKJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.250"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            169..897
                     /gene="gadW"
                     /locus_tag="ON345_003720"
     CDS             169..897
                     /gene="gadW"
                     /locus_tag="ON345_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR3073702.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1260..>3681)
                     /gene="mdtF"
                     /locus_tag="ON345_003721"
     CDS             complement(1260..>3681)
                     /gene="mdtF"
                     /locus_tag="ON345_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCR3073703.1"
                     /translation="ASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDY
                     STVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFI
                     EISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTIN
                     TLTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVL
                     SAVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKP
                     NALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEE
                     DQGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNG
                     LAFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDME
                     LLDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGV
                     ALSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMA
                     PLSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGY
                     SWTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLA
                     TDLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLR
                     PILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEH
                     LFARFKKA"
BASE COUNT          937 a          917 c          906 g          921 t
ORIGIN      
        1 attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt tgtggataaa
       61 ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctggca acatttcatt
      121 agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat gactcatgtc
      181 tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca taaaatatcg
      241 ctgcataacg agagtatcgt gctgctggag aaaaatttgg cagacgattt tgcgttttgt
      301 tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta cttacaaaat
      361 attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctatt aattaaccag
      421 tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc cggggtaaat
      481 tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc tcataagaaa
      541 gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt tgaacgcctt
      601 attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag aatgtatacc
      661 agcgagagtc tcatcaaaaa aaagttgcag gatgaaaata cctgtttcag taaaatatta
      721 ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat tcctctgcat
      781 actattgcag aaaaatgtgg ctatagcagt acgtcgtact ttataaacac atttcgacaa
      841 tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt ttcctgacat
      901 attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac tcttccctga
      961 tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg tataggtatt
     1021 ttcaggagga aaagactcgg catgtttggg attattaagc tgacaattca tacaattacg
     1081 ggaatgtggg tcagtattgt gctgttcaaa ttgatgacga atggctggag tggtttctac
     1141 tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt gagtggtgaa
     1201 tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc atttacacgt
     1261 tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac cggaacgaag
     1321 taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc taccgcgttt
     1381 tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag aataaaggcc
     1441 agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc
     1501 ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag gatggcgttc
     1561 ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc attacttaag
     1621 ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac caacatcacc
     1681 gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac cacgaccagt
     1741 gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata cgacagtccg
     1801 gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat aaatttcata
     1861 gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga cgggatgccg
     1921 ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta ggcagaaagc
     1981 ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc cggcaacata
     2041 cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt gaggaagtcg
     2101 ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga cagcgccaca
     2161 cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt atcttccagg
     2221 ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga taacagctcg
     2281 tttcgtgcct gggttagttt ttcgtgcccc aggttaccgt tgtccagcag ttccatatca
     2341 aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa gacgacggct
     2401 ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac cgagttttcc
     2461 tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt gttttgcccc
     2521 tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc tttctctttt
     2581 gtaagataat aatccgtcac ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg
     2641 gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa gaaagaggtc
     2701 ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac caccatgtag
     2761 cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt tgatttttca
     2821 aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc cggcgcggct
     2881 ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac tgaaagcagc
     2941 atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc gccgctcata
     3001 aaggccatcg gcataaacac tgcggaaaga acaacagcaa taccgaccag cgcacgttgg
     3061 atctgcccca tcgatttatg cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca
     3121 cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat cgccagcacc
     3181 atcccgaaca tagttaacgt gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc
     3241 ccgagaataa ccaccggtac ggcaatcgtc gggatgattg tggcacggaa attctgcaaa
     3301 aacagataca tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc
     3361 tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg
     3421 gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt atccagggcg
     3481 tttgctccgg tagccagttt gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc
     3541 acggtggaat aatcttccgc tccaagttcg acgcgggcga catcacgcag cagcacttgc
     3601 gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt ttgcagacgc
     3661 gtctgcacaa tgatcgaggc g
//
LOCUS       DAJSKJ010000247         3652 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000247 DAJSKJ010000000
VERSION     DAJSKJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.251"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..301)
                     /locus_tag="ON345_003722"
     CDS             complement(<1..301)
                     /locus_tag="ON345_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HCR3073704.1"
                     /translation="MFLASLLRRIAFSYYDYEAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDL"
     gene            616..1932
                     /gene="shiA"
                     /locus_tag="ON345_003723"
     CDS             616..1932
                     /gene="shiA"
                     /locus_tag="ON345_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCR3073705.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            2034..3488
                     /gene="amn"
                     /locus_tag="ON345_003724"
     CDS             2034..3488
                     /gene="amn"
                     /locus_tag="ON345_003724"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR3073706.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYIRSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          879 a          864 c          854 g         1055 t
ORIGIN      
        1 ctaaatcttt tatttctttt aatgttgcgt gatcctgcat agtcattgtg actaatgtta
       61 atttagtctg ttcaagttta agcgcattaa agacttctaa attaattgtc gacgttacaa
      121 ttaaaagatg cttaatttta tgcaattcaa gcgcccgaat aacaggaaag atggccatag
      181 catcgccaat ctgatcggga atatggatga caacaaagtc tgttttttca atattgaaat
      241 tataagcttc ataatcgtag taactaaatg caatacgtct caacaatgat gctaaaaaca
      301 tacctaacct cgcctcccta ctggttataa tgcaatgcag tctatcagac tcatcagggt
      361 gccattttgt gcatatgcgg acttttatgt ttcatatctc taacctgtgg gtcctctgct
      421 taatccttaa acaacaccag caactcctgc gctttcatct tccatcgaat ttttcatgtt
      481 gccgctaatc agccataaaa tcatttgcag atgcgctctg tcgaggtagt ctcataaggt
      541 tcgtttatag atcgacggca atgtgagtta ccttttccat actaattata aaaagacagt
      601 acaaacagga tcattatgga ctccacgctc atctccactc gtcccgatga agggacgctt
      661 tcgttaagtc gcgcccgacg agctgcgtta ggcagcttcg ctggtgccgt cgtcgactgg
      721 tatgattttt tactctatgg catcaccgcc gcactggtgt ttaatcgcga gtttttcccg
      781 caagtaagcc cggcgatggg aacgctcgcc gcatttgcca cctttggcgt cggattcctt
      841 ttccgtccgc tcggcggtgt cattttcggt cactttggcg accgactggg acgtaagcgc
      901 atgttaatgc tgaccgtctg gatgatgggt atcgcgacag ccttgattgg tattcttcct
      961 tcattctcga ccattgggtg gtgggcacct attttactgg tgacgctgcg tgccattcag
     1021 ggatttgccg tcggcggcga atggggaggc gcggcgttgc tttccgttga aagtgcacca
     1081 aaaaataaaa aagcctttta cagtagcggt gtacaagttg gctacggtgt aggtttactg
     1141 ctttcaaccg gactggtttc attgatcagt atgatgacga ctgacgaaca gtttttaagc
     1201 tggggctggc gtattccttt cctgtttagc atcgtactgg tactgggagc attgtgggtg
     1261 cgcaatggca tggaggagtc cgcggaattc gaacaacagc aatataatca agcggccgcg
     1321 aaaaaacgca tcccggttat cgaagctctg ttacgacatc ccggtgcttt cctgaagatt
     1381 attgcgctac gactgtgcga gttgctgaca atgtacatcg ttaccgcctt tgcacttaat
     1441 tattcaactc agaatatggg gttaccgcgc gaacttttcc ttaatattgg tttgctggta
     1501 ggtggattaa gctgcctgac aattccctgt tttgcctggc ttgccgatcg ttttggtcgc
     1561 cgcagggttt atatcacagg cgcgttgatc ggaacgttga gcgcatttcc tttctttatg
     1621 gcgcttgaag cacaatctat tttctggata gttttcttct ccataatgct ggcaaacatt
     1681 gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta ccgaaatgtt tggtgccagt
     1741 tatcgctata gtggtgctgg agtcggttat caggttgcca gtgtggttgg cggtggattt
     1801 acacctttta ttgccgctgc gctcatcact tactttgccg ggaactggca tagcgtcgcc
     1861 atttatttgc tagctggatg tctgatttcc gcaatgaccg ctttgttgat gaaagacaat
     1921 cagcgcgctt gatagtctgg cgaagatcat ccgatcttcg ccttgcactt ttgtttcaca
     1981 ttcctgtgac atactatcgg atgtgcggta attgtatgga acaggagaca cacatgaata
     2041 ataagggctc cggtctgacc ccagctcagg cactggataa actcgacgcg ctgtatgagc
     2101 aatctgtagt cgcattacgc aacgccattg gcaaatatat tagaagtggc gaattacctg
     2161 atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc actgaccgta acctgggacg
     2221 gtagcacaac caatcccccc aaaacgcgcg catttggtcg ttttactcac gcaggcagct
     2281 acaccaccac gattactcgc cctactctct ttcgttcgta tcttaatgaa caacttacgt
     2341 tgctgtatca ggattatggt gcgcatatct cggtgcaacc ctcgcagcat gaaatccctt
     2401 atccttatgt catcgatggc tctgaattga cacttgatcg ctcaatgagc gctgggttaa
     2461 ctcgctattt cccgacaaca gaactggcgc aaattggcga tgaaactgca gacggcattt
     2521 atcatccaac ggaattctcc ccgttatcgc attttgatgc tcgccgggtt gatttttccc
     2581 tcgcacggtt gcgccattat accggtacgc cagtagaaca ttttcaaccg ttcgtcttgt
     2641 ttaccaacta cacacgttat gtggatgaat tcgttcgttg gggatgcagc cagatcctcg
     2701 atcctgatag tccctacatt gccctttctt gtgctggcgg gaactggatc actgccgaaa
     2761 ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa aaaacaccag atgccagcat
     2821 ggcatttaat taccgctgat ggtcagggta ttactctggt gaatattggc gtgggaccgt
     2881 ccaatgctaa aaccatctgc gatcatctgg cagtgctacg cccggatgtc tggttgatga
     2941 ttggtcactg tggcggatta cgtgaaagcc aggccattgg agattatgtc cttgcacacg
     3001 cttatttacg cgatgaccac gttcttgatg cggttctgcc gcccgatatt cctattccga
     3061 gcattgctga agtgcaacgt gcgctttatg acgccaccaa gctggtgagc ggcagacccg
     3121 gtgaggaagt caaacagcgg ctacgtacag gtactgtggt aaccacagat gacaggaact
     3181 gggaattacg ttactcagct tctgcacttc gttttaactt aagccgggcc gtggcaattg
     3241 atatggaaag tgcaaccatt gccgcgcaag gatatcgttt ccgcgtgcca tacgggacac
     3301 tactgtgtgt ttcagataaa ccgttgcatg gcgagattaa acttcccggt caggctaacc
     3361 gtttttatga aggcgctatt tccgaacacc tacaaattgg cattcgggcg atcgatttgc
     3421 tgcgcgcaga aggcgaccga ctgcattcac gtaaattacg aacctttaat gagccgccgt
     3481 tccgataata agttctgaac cttcttatca aaaatagatg tgaaggatta agtaagacat
     3541 ctggcaaata gcctgcaata aagcaggctg tttgcttcct taggcggata cgagttttat
     3601 tatcgtctta atgattttca catattaaaa gcaagtatgc tttcaaaaca ca
//
LOCUS       DAJSKJ010000248         3529 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000248 DAJSKJ010000000
VERSION     DAJSKJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.252"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            75..791
                     /gene="nanC"
                     /locus_tag="ON345_003725"
     CDS             75..791
                     /gene="nanC"
                     /locus_tag="ON345_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR3073707.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            811..1917
                     /gene="nanM"
                     /locus_tag="ON345_003726"
     CDS             811..1917
                     /gene="nanM"
                     /locus_tag="ON345_003726"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR3073708.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGSRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1982..2962
                     /locus_tag="ON345_003727"
     CDS             1982..2962
                     /locus_tag="ON345_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR3073709.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(3075..>3529)
                     /locus_tag="ON345_003728"
     CDS             complement(3075..>3529)
                     /locus_tag="ON345_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3073710.1"
                     /translation="TGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPVGVMFHSDQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLKNEWV
                     PATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKKL"
BASE COUNT          987 a          788 c          835 g          919 t
ORIGIN      
        1 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
       61 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
      121 cgtcaccatt aatttctcaa gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      181 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      241 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
      301 atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc
      361 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
      421 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      481 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      541 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
      601 aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc
      661 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgtc
      721 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
      781 ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc
      841 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
      901 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg
      961 gcatggtaca agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc
     1021 cctggcggat caagagatca agcaacctct gcatttattg atggcaatct gtatgtgttt
     1081 ggcggcattg gcaaaaacag cgagggcttg actcaggtat ttaatgacgt acacaaatac
     1141 aaccccaaaa ccaatagttg ggttaaattg atgtcgcacg cgccgatggg catggcgggc
     1201 catgtgactt ttgtacacaa cggcaaggct tatgttactg gtggtgttaa ccagaatatc
     1261 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
     1321 atcaacgctc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     1381 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
     1441 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     1501 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     1561 aataagcttg ctcccgtctc atcaccagat ggcgtagctg gcggttttgc ggggataagc
     1621 aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag
     1681 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     1741 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     1801 tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg
     1861 gtgacggatt cagttttgat ctctgtgaag gataataaag tcacagtaca aaactaacgc
     1921 ttcagggccc cggtaaaggg gccctgctat caatttatca tttcaattac gcgaaaatta
     1981 tatggacgca ataatatcgc ccgattatta ctatgttctt accgttgctg gtcagtctaa
     2041 tgctatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcatcccag
     2101 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa
     2161 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     2221 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     2281 acggaaatta ctgcccttca ttcctgataa tgcagggatt ctcatcgttc catgttgccg
     2341 tggcggatcg gcttttacag cgggcagcga agggacatat tcagaacggc acggagccag
     2401 ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag tcagcagaac
     2461 gcgagccgca ctggtaaaaa acccgcagaa caaattcctc ggcgtatgct ggatgcaagg
     2521 cgaatttgac ttaatgacca gtgactacgc gtcacatcct caacacttta atcatatggt
     2581 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     2641 accatggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga
     2701 agttatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     2761 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gatccggacg atttaagcac
     2821 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     2881 tcatttcagc gcggcagccc gtcgggggat tatttctgac aggtttgtag aagcaatttt
     2941 gcagttttgg cgcgaaaggt gagcgttcat taatttgatt tattcgcatc catcagcgct
     3001 tttaactcct ccggcgtaaa cggtggtttg cgccggtgca gcattgaagt ggtcaacaaa
     3061 aactggccac cgagttagag ttttttccag tatcgatttt ccgattcgtt tggtggtaac
     3121 ccaccattat attcgtgcgg tcttagtgcg ctgtaatatc caacgatata gtccgttatt
     3181 gcgtgagctg catcgctgaa gcttacatag cccgtcgccg gcacccattc gttcttcaga
     3241 ctcctgaaga agcgctccat tgggctgtta tcccagcagt ttccacgccg actcatactc
     3301 tgcctgatcc ggtatctcca cagtaactgc cggaactgcc tgctcgtata atgactgcct
     3361 tgatcgctgt ggaacatcac cccgacgggc ttaccacggg tttcccatgc catttccagt
     3421 gctttcatgg taagcctgct gtccggcgag aacgacatgg cccagcccac tggttttctt
     3481 gcgaacaggt cgagaacaac ggcgaggtac gcccagcgct tacctgtcc
//
LOCUS       DAJSKJ010000249         3452 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000249 DAJSKJ010000000
VERSION     DAJSKJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.253"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(4..219)
                     /locus_tag="ON345_003729"
     CDS             complement(4..219)
                     /locus_tag="ON345_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HCR3073711.1"
                     /translation="MKKRILIARGSTKALLARASTEAQLDTATRFASALDSLIAHICK
                     SEMNRTEIIELLGQESEKLHNSILNQQ"
     gene            complement(285..785)
                     /locus_tag="ON345_003730"
     CDS             complement(285..785)
                     /locus_tag="ON345_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HCR3073712.1"
                     /translation="MTVMTLALVQKQPEALRVVIGKHLAEPRWQDTCDFYNQLMERDR
                     LTICFHAQLKQRHAMTRFEEMNDFERERLACAIDELRGAFSKRRQVGASESAYIGFLT
                     VAQRRTLFLHAGLTEFEFNQPYWRIDNKDCYWREKLFRALRELFSLFEYAPTVLTAVK
                     PEQYLH"
     gene            complement(952..1227)
                     /locus_tag="ON345_003731"
     CDS             complement(952..1227)
                     /locus_tag="ON345_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HCR3073713.1"
                     /translation="MNNQLVSRTDAVPYQEFARLIGKTPAAVKGMIEKGKLPVVEMTD
                     PQSTSGRAGEYWVYLPAWNKGMKMAYDSRPKEIRDGWLMWLGLGEPA"
     gene            1352..1651
                     /locus_tag="ON345_003732"
     CDS             1352..1651
                     /locus_tag="ON345_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3073714.1"
                     /translation="MSIQIHEKIKLIRESERLNRKEFSELTGIVYGSFCSYEAGDKKP
                     GIEQIMKILQHPRFTKYTLWFMTDQITPEAGQIAPALAHFGQQTTTSPHSDQKTG"
     gene            1748..2758
                     /locus_tag="ON345_003733"
     CDS             1748..2758
                     /locus_tag="ON345_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3073715.1"
                     /translation="MSVKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAMAFEKHTQY
                     NHHSKEWLSKPTDKRQLSELKELWWNLKGKHEEHGQSYLRKIERFETMTGNPCAFQIT
                     KSLITQYCAQRRGEGIKPTTINRDLITLGGMFTTLIESELYNGEHPFRGFKKLKEQTA
                     ETGYLTLEEIDALLAALSGDNRKIAVLCLSTGARWGEAARLKAENVIHNRVSFVKTKT
                     NTPRTVPISDDVAAYIVGKTRGFLFSDASYAEFRLILKAVKPDLPAGQATHALRHSFA
                     THFMINGGNIITLQRILGHTKIAQTMVYAHFAPQYLQDAISLNPLKGANGGQSVHNVS
                     TP"
     gene            complement(2866..3054)
                     /locus_tag="ON345_003734"
     CDS             complement(2866..3054)
                     /locus_tag="ON345_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3073716.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(3065..3277)
                     /gene="cspI"
                     /locus_tag="ON345_003735"
     CDS             complement(3065..3277)
                     /gene="cspI"
                     /locus_tag="ON345_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HCR3073717.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT         1022 a          771 c          759 g          900 t
ORIGIN      
        1 gttttattgt tggtttaaaa ttgagttgtg aagcttttct gactcctgcc ccaacagctc
       61 aatgatttcc gtccgattca tttcagattt gcaaatatgc gctatcaagg aatcaagcgc
      121 tgatgcgaaa cgagtcgccg tgtctagttg cgcctcagtt gaagcgcgcg ccagcagagc
      181 cttagtgcta ccgcgtgcga ttaaaattct ctttttcatt tgccctacgc cagataaaaa
      241 gatgccccac gtagttaaac gcgttaaaag aaactggtat ttaattaatg caaatactgc
      301 tcaggcttaa ccgctgttaa tactgtcggg gcatattcaa acaaactaaa taattcacgt
      361 aacgcacgga ataatttttc acgccagtag caatctttgt tgtcaatacg ccagtaaggc
      421 tgattaaatt caaactcagt taatccagca tgtagaaata aagttcggcg ctgagcaacg
      481 gttaaaaacc caatatatgc tgactcactg gctccgacct gacgacgttt agaaaatgcg
      541 cccctgagtt catcaatcgc acaagccaaa cgctcgcgtt cgaagtcgtt catttcctcg
      601 aaacgtgtca tcgcgtgacg ttgttttaac tgcgcatgaa aacagatagt aaggcgatca
      661 cgttccatca gttgattgta gaagtcgcat gtatcctgcc agcgaggctc cgcaagatgt
      721 ttaccaataa caactcgcag agcttcaggt tgtttttgaa ctaacgcaag agtcataacc
      781 gtcataaagc caaccctcgg gatttaataa tgcgcttaaa gtcggcaaag atgcccggct
      841 tacgcgtgcg gatgatgatg cccttacgac cgcgaccatg tgtgatatga aagtcaatcg
      901 ggatagggct ttcagtgcgg agtaactgag cgatgcagcg atgttctttc attaagcagg
      961 ctctcccaac cctaaccaca taagccaacc gtcgcggatt tctttaggac gactgtcata
     1021 ggccattttc atgcccttat tccatgcggg aaggtaaacc cagtattccc ctgctcggcc
     1081 gctggttgat tggggatcgg tcatctcaac cacaggaagc ttacccttct caatcattcc
     1141 cttaactgct gcaggtgttt tgccaattag acgggcaaac tcctgatatg gaaccgcatc
     1201 tgttctgctt acaagctggt tgttcatctg ctacgattct cctttagtgt gtttaattgc
     1261 tctaaatagg gattaattgc tctaaaaggt gattcaccta tcggctatat aaatctacga
     1321 taggtgataa tgttcaacta taggtgattt tatgtcaata cagatccatg aaaaaatcaa
     1381 gctgataaga gagtcagaaa ggttaaacag gaaagaattc agtgagttaa ctggtattgt
     1441 ttacggttct ttttgcagtt atgaagctgg cgataaaaag ccggggattg aacaaatcat
     1501 gaaaatcctt caacacccgc gatttacgaa gtacacgctg tggtttatga ctgatcaaat
     1561 aacacccgaa gctgggcaga ttgcaccggc tctcgcgcac tttgggcagc agacaacaac
     1621 gtcaccccac tcagaccaga aaactggcta accatttacg gcgcttattt gtgcagtaaa
     1681 tgcacagtga gtttttgtta tgtaaatcag gaaattgaag tacgtagtaa catcatcggg
     1741 aggctttatg tctgttaaaa agctcgatga tggtcgatat gaagtggaca ttagaccgac
     1801 cgggcgtaac ggaaaacgca tccgtcggaa gttcgacaag aaaagcgagg cgatggcttt
     1861 tgaaaagcac actcaatata accatcactc aaaggaatgg ctttcaaaac caacggacaa
     1921 acgccaactg tcagaactga aagagttatg gtggaacttg aaagggaaac acgaggaaca
     1981 tggtcaatcg tatctcagga aaattgagcg tttcgaaacg atgaccggca atccatgtgc
     2041 tttccagata accaaaagcc tgataacgca atattgtgct cagcgccggg gtgaaggcat
     2101 caagccaacc actatcaacc gtgacctgat cactttgggt gggatgttca ctaccttgat
     2161 tgagtctgag ttgtataacg gtgagcatcc gttcagaggg ttcaaaaagc taaaagagca
     2221 gaccgccgaa acgggctatc tcactcttga ggaaattgat gccttactgg cagcgctctc
     2281 aggtgataat cgtaaaatcg ccgttttgtg cctgagcaca ggagcaagat ggggagaagc
     2341 tgcgcgactg aaagcggaga acgtgattca taaccgggtg tctttcgtta agacgaaaac
     2401 caacacaccg cgtacggttc cgatctctga tgatgttgca gcttacatag tcggtaaaac
     2461 gagagggttt ctgttcagtg atgccagtta tgctgagttc aggctaatcc tcaaagcggt
     2521 taaacccgac ttaccagccg ggcaagcgac acatgcgtta cggcactctt tcgccacgca
     2581 ctttatgatt aacgggggca acatcatcac actgcagagg atcttaggtc atacgaaaat
     2641 tgctcagaca atggtctatg cgcatttcgc tcctcagtac ctgcaggacg cgatttcgct
     2701 taacccgttg aagggtgcaa atggtggtca gagtgtccac aatgtgtcca caccctagcc
     2761 gctttttatg gcttttggct gcttgtagta aaacgtgaag ccttgtctgg cgcggctttc
     2821 cagctacgcc aggcataaaa aaggctcccg aaggagcctt cattttcact tttttaaatc
     2881 caacgacaga cggctggcat ttaagtattg tgaaatatta tcaaatgtaa tcatcattga
     2941 tttacaaaag atacattttg ccccgaaagg attcatgtca gaaacatcaa aagatgatgt
     3001 tctatactgg gaaccatgac aacacgggca tctaaagtga atatggtttg taatattgtc
     3061 tacctcaaag cgccactaca tgaacagcgg caggaccttt aggtccgttc tcaataccaa
     3121 attcaacttc ctgattctca gttaatgttt tgaaatcgtt gctctgaatt gctgagaaat
     3181 ggacaaacac atctttgctg ccatctttcg gcgtgatgaa accaaaacct ttttcagggt
     3241 taaaccattt cactaaacca gtcattttgt tagacataat tattaccttt tgaagaaatt
     3301 agcccttggg cagaatggtc cgaaaaaaaa tatcagagag aaaaaccaac aaggaaatct
     3361 caagaggtac aaataataaa attataacaa tgactgcttc agataaattt gtaacaaacc
     3421 agaacaccat taacgcatga ttaaccaccc at
//
LOCUS       DAJSKJ010000250         3375 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000250 DAJSKJ010000000
VERSION     DAJSKJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.254"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(451..1701)
                     /locus_tag="ON345_003736"
                     /pseudo
     CDS             complement(451..1701)
                     /locus_tag="ON345_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(1706..2200)
                     /locus_tag="ON345_003737"
     CDS             complement(1706..2200)
                     /locus_tag="ON345_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HCR3073718.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILESDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            complement(2763..2969)
                     /locus_tag="ON345_003738"
     CDS             complement(2763..2969)
                     /locus_tag="ON345_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073719.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            3270..>3375
                     /locus_tag="ON345_003739"
     CDS             3270..>3375
                     /locus_tag="ON345_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCR3073720.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLY"
BASE COUNT          750 a          894 c          807 g          924 t
ORIGIN      
        1 ccgacctttt gaagacagat taactgatga tactgctgtt ccacctgacg gtaacgcccc
       61 gatctctgat gccgttggct tatttctgga gttatagtcc cttcgccagc caggtgaata
      121 atctgttccg tgattaatat aggtaaactg ggcgttagtt gttccaccac cagtggaggt
      181 ggtcggtgtg gtaatgcgga tcgtcatcgc tgactttatc cccattactt caatgacagc
      241 tccggcgaga tgaatattac cgcagccagt atcagtaatg attttattat tgccataaga
      301 ccaggaaccc ttgcacatcc agtatggatg gttaaatgct ccctgagaat ccagccactc
      361 gataaactgt gcagtcgtcc agtttcctat tgttgtgctt actgacccac cgaaggcacg
      421 gcaggcacca atatttttcg taaaggtgtc tttgccaggg atatccgcac cgttctgatc
      481 tttctgcaga cgtttctcag cattgtcatt ggctgctttt actgcctttg gcgttgccgc
      541 cagcgtttca gacgtgctgt tggtcgcgct gcttagctgg attatccctt tctgtgctgt
      601 cgttgcatcc tgtgcggtgt atttcccgtt agccaggtca tacgcggcct taacggcttt
      661 tggcgttgcc gccagtgact cggaagtgct gttggtcgca ctgctgagct gtactatccc
      721 ctttttcgtc gtgctcgcat cctcaagcgc cacggcggat gcaatatcct ctgcccgttt
      781 tgccgctgtc tcagcgcgcg ttgctgcaga ttccgccgta cttttgctct gagctgctgc
      841 cgtcgcactg ccagctgcct ctgtcgcctt cgtggatgcc gtcgtggcgc tacccttcgc
      901 tgctgacgcc tgtctggtcg cctcatcttt tgaagcagac gcagatgatg ccgatgacga
      961 cgccgaactg gctgacgatg cggcagccgt ttttgaggat tctgcgctgg tttccgacgc
     1021 tttcgcgttc gtttcggatg tcttcgctgc ggaagcagac ctcgctgctg cgctggcctg
     1081 ttcagtggct tcgccagcct tcgttgtggc tgttgaagca gacgatgcgg cgctttctgc
     1141 cgattttccg gcggcggtgg cactggctga ggcctgcccg gcacttgttg acgctgcact
     1201 ggcagacgac gcagccgctg tttttgagcc tgccgcagcc gaggcgctct gtcccgctgc
     1261 cgtttcagaa gacctggcgt tcgtctctga cgtttttgcc gccttcgcgg aatttcctgc
     1321 cgctgttgcc gaggaagctg cgctactggc gctcgaggat gcgttcgttt ctgatgattt
     1381 tgccgcctct tttgaacccg ccgcatcccg ggctgaggtg gcagcttctg acgctttcgt
     1441 agtcgcggtg gatgcagaag tggctgctga ttgttgtgac gctgcagcat tcgtttctga
     1501 cgttttcgcg gcactggcac tggtagctgc cgcgcttttt gaggactctg cagcggcagc
     1561 actttttgat gcttcagtgg cctttgttga tgccgttcct gcgctggaag tcgctgactg
     1621 agccgacgac gcggcctgtc cggctgacgt gctggctgcg cgtgctgagc ctgcagcatc
     1681 agtcgcatgg gttcctgatt cagcattact cctcctgctg taattcctcc tcatcatccg
     1741 cacctgcttc agtcacccgc agggctatct gatcgcgcag atcatcaata atggactgaa
     1801 cacggctcac agcggcaggc tgcagaccgc agtcactttc aagaatatcc ggtaatgtct
     1861 ccagcacctg cacgaccgct tttgcccaga tggcaaactc ccgtctgaca tcactggccg
     1921 gaatgagttg tgctgtttcc tgttcgaact taagacgctc acgttcagac tgataccagg
     1981 ctttgcgctc atgcgcgtcc atttcgcctt ctgcaaccgg cggtggtaat gccagaaatg
     2041 ccgacacaat atcaaccacc cgataaagct tgaggttgct ttcatgcccc cctgcaacgg
     2101 gtagattttg cagccttgcc gcagcagtct ggcgatgtac acctgacaat gccgccagtt
     2161 gactgatatt cagcgtcaga ttttttaact ctcgatccat acccgctcca gaatgtttta
     2221 aacatgcatc ttgcgaacaa ctttaggcaa acggtgttag tgatgaacaa aaaacaatca
     2281 ggatcgacac cataaaaata aaaccacttt aatatcaatc aattacagta gtggtgatga
     2341 cgaataaaat ttcaaaaact agcctttttc cgcgacgctc ccgccccgtg gcaggggccc
     2401 ccaccgggag gacccgacag cctgacagcc tgacagccgt gatgagcatc tgatacagcg
     2461 ctttacataa tggcatagga ataattcaga aggacatcac agcatgccca cacaaattag
     2521 tgtgagtgtc ctgtttcttc ctacgcacag gactgacgag catgagggga aaatatgcga
     2581 accataaatg cctcatcttc agcaatgcaa ccggcatccg aacaggacga tattgtaaat
     2641 tcacctaatt acgaggacat tgcagtagtt gaattgcagc tctgtattag cctgacagtg
     2701 acagaatgcg atattgactc tgtcacaggt gaaatagttt gaatgattag cagttatggt
     2761 gctcagtcaa ccaccaggga ataatccttc gaattcttat cgtgcttcac caacgctgcc
     2821 tcaattgccc tgaatgcttc cagagacacc tgatgttcta tacatgcaat tacaacatcc
     2881 gggtaactca tagaaatggt gctattaagc atatttttta cacgaatcag atccaacgag
     2941 agttcatcag cagattgttc tttattcatt ttgtcgctcc atgcgtttgc tcgtcatcta
     3001 gcggttaaaa tattacttca aatcttactg cataaaagag gaaaagtatg aaagtttgag
     3061 tacatcgacc ttacatacat ctgtcggttg catatccctc ctggatgcca gcaaggctca
     3121 attttgttac gcaatcaaca ctattcatta aaaacaggct taatatttga cataaatcat
     3181 caacaaaaca caaagaggtc agaccagatt gaaacaataa acacgataat gctaactacg
     3241 cgccatcgta tcacatggaa ggtttaccaa tggctcaggc tgccattttt aaagaaatat
     3301 tcgatcaagt gcgtaaagat ttaaactgtg aattatttta ttctgagcta aaacgtcaca
     3361 atgtctcact ttata
//
LOCUS       DAJSKJ010000251         3374 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000251 DAJSKJ010000000
VERSION     DAJSKJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.255"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            211..1143
                     /gene="rfaD"
                     /locus_tag="ON345_003740"
     CDS             211..1143
                     /gene="rfaD"
                     /locus_tag="ON345_003740"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR3073721.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1153..2199
                     /gene="rfaF"
                     /locus_tag="ON345_003741"
     CDS             1153..2199
                     /gene="rfaF"
                     /locus_tag="ON345_003741"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR3073722.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2203..3195
                     /gene="rfaC"
                     /locus_tag="ON345_003742"
     CDS             2203..3195
                     /gene="rfaC"
                     /locus_tag="ON345_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR3073723.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLISEIR"
     gene            3192..>3374
                     /locus_tag="ON345_003743"
     CDS             3192..>3374
                     /locus_tag="ON345_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR3073724.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHY"
BASE COUNT          862 a          821 c          878 g          813 t
ORIGIN      
        1 tatcatacat cggaatattg atactaaagc actattcaca tgcaaaacca acatccgcca
       61 tgaaggacta gctaaaaccc aaactagttt gttgcaatta gcatccttgc acctctatgt
      121 aaagggctga agggattcgg atgtgatggt atgattacag acattcgtgt ctgagattgt
      181 ctctgactcc ataattcgaa ggttacagtt atgatcatcg ttaccggcgg cgcgggcttt
      241 atcggcagca acatcgttaa agccctgaat gataaaggca tcaccgatat tctggtggtg
      301 gacaacctga aagacggcac caagtttgtg aacctggtgg atctgaatat cgcagactat
      361 atggataagg aagacttcct gatccagatt atggctggcg aagagttcgg cgatgtcgaa
      421 gcgattttcc acgaaggcgc gtgctcttcc accaccgagt gggacggcaa gtatatgatg
      481 gataacaact atcaatactc caaggagctg ctgcactact gcctggagcg tgaaatcccg
      541 ttcctgtacg cttcttccgc agccacctac ggcggacgca cctccgactt tattgaatcc
      601 cgcgagtacg aaaaaccgtt gaacgtctac ggttactcaa aattcctgtt tgatgaatat
      661 gttcgccaaa tcctgccgga agcgaactcg cagattgttg gcttccgcta tttcaacgtt
      721 tatggaccgc gtgaaggcca taaaggcagc atggcgagcg tcgctttcca tctcaacacc
      781 cagcttaata acggtgaatc accgaagctg ttcgaaggta gcgagaactt taaacgcgac
      841 ttcgtctatg ttggcgatgt ggcagatgta aacctgtggt tcctggaaaa tggcgtttcc
      901 ggcatcttca acctcggcac cggtcgtgcg gaatccttcc aggctgtagc tgatgctacg
      961 ctggcttatc acaagaaagg ccagatcgaa tacattccgt tcccggataa gctgaaaggc
     1021 cgctaccagg cgttcactca ggcagatctg acaaatctgc gcgcggcggg ttacgacaaa
     1081 ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg cctggctgaa tcgcgacgca
     1141 taagagctct gcatgaaaat actggtgatc ggcccgtctt gggttggcga catgatgatg
     1201 tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc aggcgataat cgatgtgatg
     1261 gcaccggcat ggtgccgtcc attattatcg cggatgccgg aagttaacga agctatccct
     1321 atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc gcaaactggg tcatagcctg
     1381 cgtgaaaagc gctacgaccg cgcctacgtc ttacctaact ccttcaaatc cgcattagtg
     1441 cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg gcgagatgcg ctacggttta
     1501 ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc taatggtgga acgctatata
     1561 gcgctggcct atgacaaagg cattatgcgc acagcacaag atctgccgca gccattgtta
     1621 tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata cctgtaatca attttcgctt
     1681 tcatcagaac gtccgatgat tggtttttgc ccgggtgcgg agtttggtcc ggcaaaacgc
     1741 tggccacact accactatgc ggagctggca aagcagctga ttgatgaagg ttatcaggtg
     1801 gttctgtttg gctcggcgaa agatcatgaa gcaggcaatg agattcttgc cgctttgaat
     1861 accgagcagc aggcatggtg tcggaacctg gcgggggaaa cacagcttga tcaagcggtt
     1921 atcctgattg cagcctgtaa agccattgtc actaacgatt ctggcctgat gcacgttgcg
     1981 gcggcgctca atcgtccgct ggttgccctg tatggtccga gtagcccgga cttcacaccg
     2041 ccgctatccc ataaagcgcg cgtgatccgc ctgattaccg gctatcacaa agtgcgtaaa
     2101 ggtgacgctg cggagggtta tcaccagagc ttgatcgaca ttactcccca gcgcgtactg
     2161 gaagaactca acgcgctatt gttacaagag gaagcctgac ggatgcgggt tttgatcgtt
     2221 aaaacatcgt cgatgggcga tgttctccat acgttgcccg cactcactga tgcccagcag
     2281 gcaatcccag aaattaagtt tgactgggtg gtggaagaag ggttcgcaca gattccttcc
     2341 tggcacgctg ccgttgagcg agttattcct gtggcaatac gtcgctggcg taaagcctgg
     2401 ttctcggccc ctataaaagc tgaacgcaaa gcgtttcgtg aagcgctaca agcagagaac
     2461 tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg cggcgctggt gacgcgtctg
     2521 gcgcatggcg taaagcatgg catggactgg caaaccgctc gcgaaccttt agccagcctg
     2581 ttttacaatc gtaagcatca tattgcaaaa cagcagcacg ccgtagaacg cacccgcgaa
     2641 ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc agggcgatta tgctatcgca
     2701 cagcattttc tgacgaacct gcctacagat gctggcgaat atgccgtatt tcttcatgcg
     2761 acgacccgtg atgataaaca ctggccggaa gaacactggc gagaattgat tggtttactg
     2821 gctgattcag gaatacggat taaacttccg tggggcgcgc cgcatgagga agaacgggcg
     2881 aaacgactgg cggaaggatt tgcttatgtt gaagtattgc cgaagatgag tctggaaggc
     2941 gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg tggatacggg gttaagccat
     3001 ttaacggcgg cactggatag acccaatatc acggtttatg gaccaaccga tccgggatta
     3061 attggtgggt atgggaagaa tcaagtagag tgtcgttcta caagtatgtc tcttgcagat
     3121 ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa taataaccaa taagttgata
     3181 tcggagataa gatgacctca acattatttt tctctctcga gaaaaaaaac tggatagcgt
     3241 actggaacag agctctcgta ttcttattca ttaccaccta ttttttgggt gggattacaa
     3301 ggtataaaca tcttattgtt attcttatga caataacgac aatcgtctat ctctgcaaac
     3361 ggccaaaaca ctat
//
LOCUS       DAJSKJ010000252         3366 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000252 DAJSKJ010000000
VERSION     DAJSKJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.256"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            6..248
                     /locus_tag="ON345_003744"
     CDS             6..248
                     /locus_tag="ON345_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073725.1"
                     /translation="MSFKPTPEEVKQARIKAGFTQQEAAERFGFTLSAWQAKETSGKT
                     SRGLAAGTYELLLLLADEHPDYQLVKREKNQDKVTK"
     gene            342..680
                     /locus_tag="ON345_003745"
     CDS             342..680
                     /locus_tag="ON345_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073726.1"
                     /translation="MNTYVITFQALNTKKEKITCSAFVHSETLFLAVHSFEDKNRGRG
                     YVITSVSELLSEELTAKNSLKKNINFWFNECGLSKSEVINEVINWKNFAYTLKELEEA
                     KNEAIRELRN"
     gene            755..919
                     /locus_tag="ON345_003746"
     CDS             755..919
                     /locus_tag="ON345_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073727.1"
                     /translation="MSERMLSAIQTVEKGGRPVFPLMPFSAFPEYMALLRKALEKKET
                     KALIEKQEVL"
     gene            916..1173
                     /locus_tag="ON345_003747"
     CDS             916..1173
                     /locus_tag="ON345_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073728.1"
                     /translation="MKKQEFLDFISAEQRRGAVRFSLGFNSKGEIVLHWTNEAGLRVW
                     SILSGNRGKSPSRANRERMSNLRRWLHDARQGMEGDTPEAE"
     gene            1445..1519
                     /locus_tag="ON345_003748"
                     /pseudo
     CDS             1445..1519
                     /locus_tag="ON345_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
     gene            1514..2059
                     /locus_tag="ON345_003749"
     CDS             1514..2059
                     /locus_tag="ON345_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HCR3073729.1"
                     /translation="MKKVQFRIEESQHDDLLDCLKTLYPDEPGLTVAKGMKLLANALL
                     KSKVKNEDVNVSKDNDDFIKTTMYLTGKQRTLIEKAAHRHGWNLSRECRYRIQTTLEN
                     ELDFFDQELLVMNRCRNAIDKIGRNFHYIIVNDNARVLDKDGFYQDAERLSSEIMSLK
                     SEFENYIMLCKGRTVSNKVEV"
     gene            2062..3222
                     /locus_tag="ON345_003750"
     CDS             2062..3222
                     /locus_tag="ON345_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3073730.1"
                     /translation="MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERIN
                     RKGASKEVVVKISGGAITRQGVRNSIDYMSRESELPVMSESGQVWKGDEIQEAKEHMI
                     DRANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPEDLLESVRKTMNKKYPNHRFVL
                     GYHNDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYDVKATHKQQ
                     HGLNQSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYW
                     GADFGELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGV
                     KGIDRISSSSKELVLNSAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG"
BASE COUNT         1084 a          617 c          812 g          853 t
ORIGIN      
        1 aaatcatgag ttttaagcca acacctgaag aggtgaaaca agcacgtatt aaagctggct
       61 ttacgcagca ggaagccgct gaacggtttg gttttacgtt atcagcctgg caagccaaag
      121 aaacatccgg taaaacgtct cgtggtttag ccgctggtac gtatgaatta cttttgctcc
      181 tggctgatga acatccagat taccagctcg ttaagagaga aaaaaatcag gataaagtga
      241 ctaaataaat tgcattacca tcatatgatg gtataattta tttgtaggtc gggaatagtt
      301 ccttaccatg atccagtagc ccaaacgggc gggagactaa aatgaacacg tatgttatca
      361 ctttccaggc tttaaacact aagaaagaaa aaatcacctg ctctgctttt gtccattcag
      421 aaactctctt ccttgccgtt cattctttcg aagataaaaa tcgcggtcgt ggttatgtta
      481 tcacgtctgt tagtgaattg ttgtccgagg aactgacggc gaaaaattcc ctcaagaaaa
      541 atattaactt ttggtttaat gagtgtggtt taagtaaatc tgaggttata aacgaagtaa
      601 ttaactggaa gaatttcgca tataccctta aagaactgga ggaagccaaa aatgaagcga
      661 taagagaact gcggaattaa tcaattaggg gtggtatcag gttgtggagg caacctgata
      721 ccgtgacact caattccctc gcgaggaata taaaatgtcg gaacgcatgt tatcagcaat
      781 acagactgtt gaaaagggtg ggcgtccggt ttttcccttg atgccattct ctgcttttcc
      841 tgagtacatg gcattactca gaaaagccct ggaaaagaaa gaaacaaaag cactgataga
      901 aaaacaggag gtgctatgaa aaaacaggag tttttagatt ttatcagtgc agaacagagg
      961 cggggagctg tccggttttc gctgggattt aacagcaaag gggaaattgt attgcactgg
     1021 actaacgagg ccgggttaag agtctggagt atactaagcg gtaacagggg aaaaagtccc
     1081 agccgggcaa accgggaaag aatgagtaac ctccgccgct ggctccatga tgcccggcag
     1141 ggcatggaag gcgacacacc agaggcagaa taaaattccc gttcctgaat cgccgaagcc
     1201 ctcccgttta cgggaggtgc tttttttgta tattccgtaa aacagcgctc gcgccccctg
     1261 ccaggccatt cgcgtagcga atttatttcc gcccaagtta gccgcgaatc cgttgtctcc
     1321 ggtcgcagac gcaccagatc acagccacga tagcctttca ttttttgcat ttcgatggac
     1381 gctgaagcac aatttatgtg tggaggtagt tcactgcgat gcaggatagg gcaaacgccg
     1441 caaaatgacg tctctgacgc cattccgcaa cgtttgcccg gtgacctaca gtcggttctt
     1501 gttggagagt tttatgaaaa aagttcaatt caggattgaa gaaagccaac atgatgatct
     1561 acttgattgt cttaaaacac tttatccaga tgaaccgggt ttaaccgtgg caaaaggaat
     1621 gaagctgctt gccaatgctt tactgaaaag caaggttaaa aatgaagatg ttaatgtgtc
     1681 aaaagacaat gatgatttta tcaaaaccac aatgtatttg acagggaaac aaagaacttt
     1741 aatagagaaa gcggctcatc gtcatggctg gaatttatcc agagaatgtc gttatcgaat
     1801 ccagacgaca cttgaaaatg aacttgattt tttcgatcag gaacttctgg tgatgaatcg
     1861 ttgccgtaat gcaatagata aaatagggcg aaactttcat tatattatcg ttaatgataa
     1921 tgccagagta cttgataaag atgggttcta tcaggacgct gagcggttga gttcagagat
     1981 aatgagtctc aaaagtgagt ttgaaaacta cattatgtta tgcaaagggc gaactgtttc
     2041 taacaaggtt gaggtgtaat catgggcgtt tatgttgaca aggaattcag ggttaagcgt
     2101 aagtcctctg aagctggccg caagtctgct ttcgcccata aagttaaaaa tggtggtaaa
     2161 aactatcaac gcaacgttca ggaacgtatc aaccgcaagg gtgccagtaa ggaggttgtt
     2221 gtcaaaatct caggaggtgc gattacccgg cagggagttc gaaacagtat tgactatatg
     2281 agccgggagt cagagctgcc agtgatgagt gaaagcggtc aggtatggaa aggtgatgag
     2341 attcaggagg ctaaagagca catgatagat cgtgctaatg accctcaaaa tgttttcgat
     2401 gataaaggta aggaaaataa aaaagtaacc caaaatattg tgttttcacc gcctgtatca
     2461 gcaaaggtaa aacctgaaga tctgcttgag tctgtcagga aaacgatgaa caagaagtac
     2521 ccaaatcatc gttttgtgct tggctatcat aacgacaaaa aagaacatcc acacgtgcat
     2581 gtcgtttttc gtattcgtga taatgacggt aaacgagctg atatcaggaa aaaagattta
     2641 cgggaaattc gtacaggttt ttgtgaagag ctgaagatga agggctatga cgttaaagcc
     2701 actcataagc agcagcatgg acttaaccag tctattaaag atgcgcacaa aacagcgcca
     2761 aaacgacaga agggcgttta tgaggttgtt gatgttggct acgaccatta tcagaacgat
     2821 aaaaccaaac caaagcagca tttcataaag cttaagacac tgaacaaggg cgtggagaaa
     2881 acatattggg gggctgattt tggtgagtta accactcgcg agaacgttaa gaaaggtgat
     2941 cttgttaagc tgaagaagtt aggacaaaag gaggtgaaaa tccctgcact tgataagaac
     3001 ggtgtacagc atggctggaa gactgcacac aggaatgagt ggcagctaga gaacctgggg
     3061 gttaagggca tagacagaat ctcttcatcc agtaaagagc tggtgctgaa cagcgcggag
     3121 atgattaaga aacagcaact ccagatgaga aatttctcgc aaataaaaca gtcgatgatt
     3181 caaagcgaac agaaagtgaa aatcggaatt cgtttaggat aattcatact ctccggtaac
     3241 ttattgatta agatcgattt atttatcttg ttagcagagt ttttgtaaaa agtaactagc
     3301 ggtaaaaaat ttagttaact tgactataaa ataagcgcat ggtgatcaat cgatctgctt
     3361 ttttgt
//
LOCUS       DAJSKJ010000253         3344 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000253 DAJSKJ010000000
VERSION     DAJSKJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.257"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            505..804
                     /locus_tag="ON345_003751"
     CDS             505..804
                     /locus_tag="ON345_003751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073731.1"
                     /translation="MEKRDNMLRVRFSDAEFEALKQLAEDAGCTMSELVRDHLGRVSV
                     RNKDVDRERIAMLNRINANLNMIARWVNTHKSAASSVEVVAHLMDIERNIWELSR"
     gene            801..1553
                     /locus_tag="ON345_003752"
                     /pseudo
     CDS             801..1553
                     /locus_tag="ON345_003752"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1537..2043
                     /locus_tag="ON345_003753"
     CDS             1537..2043
                     /locus_tag="ON345_003753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR3073732.1"
                     /translation="MSVSLTLQERLKQQHLAESETIQSLTKQQLSNMRSELNGIFQQE
                     LNTMLEDMRRQLHDTTEDMRQSRQSMLLTIVKNRVIFPALSAVTILAVIFLSGWGLST
                     FQESQIMANWQQINSQKATLVALESKTAGLNIVQDKNGTFVVLPKGAKVDNSGWTVGK
                     QQALRIVR"
     gene            2047..2280
                     /locus_tag="ON345_003754"
     CDS             2047..2280
                     /locus_tag="ON345_003754"
                     /inference="COORDINATES: protein motif:HMM:NF016769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR3073733.1"
                     /translation="MTELETHLLNALKRMEQQFSEQLKASEKAQTALQSMFERTAEDN
                     ATLLKQVSFLDKRVTVLSEQLKQFGNLYKQNRR"
     gene            complement(2416..2823)
                     /locus_tag="ON345_003755"
     CDS             complement(2416..2823)
                     /locus_tag="ON345_003755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073734.1"
                     /translation="MFSGIAEIFYISKVETIVDTELRLLPNLFKLQVPDLAHVIIINQ
                     YRAMPDVFAKHKGKRLMPEAVALHSTLTFMKNYSSENSLVIQGEDCLGALKIVCLRLA
                     KRAASLPTTQMEAAFMQEEILPVFLSFLQKDEL"
     gene            complement(2851..3282)
                     /locus_tag="ON345_003756"
     CDS             complement(2851..3282)
                     /locus_tag="ON345_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3073735.1"
                     /translation="MRRVTQVDLETGEDLGGFVAVVRPKQKSSFQRHFTMNQAALITI
                     ANELNHDQMRVLMALLAELDYENYIQVAQIDIAEALRMQKTNVSRAIKNLIEFGIILE
                     GPKIGRSKTYRLNPQFGWKGTVSNHKKALSNGLSVIQGGKY"
BASE COUNT          864 a          801 c          907 g          772 t
ORIGIN      
        1 aaaaaaaagc taggactgat gcggcttgcg ggagagtccc ttctgtatct tgttacttgc
       61 ctccagtgtt cacagcgtat gccatgccga gcgaacacat ttttatgccc gttcaggtga
      121 tattccgctt cctcgttccg gctgttcgtt cctcgctgcg ctgcggtacg cctcagccgg
      181 aactgcggcg agcggtacta gctcattcaa aaggcgcagg cattgcctga cttcgtcaag
      241 ggcgagcgga gcgagtgcat ttcacccttg acgacgctac ggacaaaaca cgcttttcag
      301 ggggggatgg tttcacgcag tgaggccatc cacctgacag acgccgttgc cctgggcgag
      361 ggagcagagc gaccgctgca agggaggggt caaggggagg cgaagccgcc cattgcaagg
      421 cctggtgtgc tacattgagc gtagcgaaat gtagcaacat cgggtagcct tgtttaaaga
      481 gcaatatgcg acgacggaga tgagatggaa aagcgcgata acatgctgcg ggtcaggttc
      541 tctgacgcgg aatttgaggc gctgaaacag ctggccgaag atgcaggttg cactatgtcc
      601 gagctggttc gcgatcatct cggcagggtc agtgtgcgaa acaaagacgt cgatcgggag
      661 cggattgcca tgctgaaccg catcaatgcc aatctgaata tgattgcccg ctgggttaat
      721 acgcataaat cggcagcttc ctcggttgag gtcgttgctc acctgatgga tatcgagcgc
      781 aatatttggg aactgtcgcg atgattgtgg gtttcagcag gcacggcact ggcggcggcg
      841 gtggccccgt cgactatgtc accgataaaa cgcggcccgg tcgggaagat tcaccaccgg
      901 aagtacttag gggatccccg gaggctaccc gcaacctcat cgactcgctc gatttccggc
      961 acaaatacac ttctggtgtg ctgtctttcg ccccagagga gaaggtcacg ccggaaatgg
     1021 agcaggagtt catggatcgc tttgagcagg tcgcgttcgc cgggcttgaa gccgatcagt
     1081 acaacattct ctgggtcagg catacccatg ccggacatca cgagctgcat tttgtgacgc
     1141 cccgcgttga gcttggcaca gggaaaagcc tgaacatccg cccgcccgga gatgccgcaa
     1201 aacaggcttt cgatgatctg cgcagtgaaa tcaatgctag gtatggcctg gctgacccgg
     1261 atgacccacg gcgcgcccgt gagattgctc agcctgatca tgagctgaaa atcgccgcag
     1321 aagccctcag aaggggcgag aagccgcagg aagacgtcag gctactcata gacgcggttt
     1381 tgtctcagag ggccgcacag gggcttatac gcagtcgtag cgacgttatc gagcaggttt
     1441 cagaccttgg aatgcaggta acgcgcgaag gtaaaaatta catcaccgtg tcagatccgc
     1501 agagcggcca gcggtggcgg atgaaagggg gactttatga gcgtgagttt gacgctgcag
     1561 gagcgattga agcaacagca tctcgcagag agcgagacta ttcaaagcct gacgaagcag
     1621 cagctgagca atatgcgaag cgagttgaac ggcatatttc agcaagagct gaataccatg
     1681 ctggaagata tgcgccggca gctgcacgac acgacagagg atatgcgcca gagccggcag
     1741 agcatgttgc tgaccatcgt gaaaaacagg gtgatattcc cggccctgag cgcggttacg
     1801 atcttggccg ttatctttct gagcggctgg ggcctgagta cgtttcagga aagccaaatc
     1861 atggccaact ggcagcagat caacagccag aaagccacgt tagtggcgct ggagagcaaa
     1921 acagcgggcc tgaacatcgt tcaggacaag aacgggacgt tcgttgtgct gcccaaaggg
     1981 gcgaaagtag acaacagcgg ctggacagtc gggaagcagc aagctctgcg gatcgtgaga
     2041 taaaccatga cggaactgga aacgcactta ttgaacgcct tgaagcgtat ggagcagcag
     2101 ttcagcgagc agctgaaagc ttccgagaag gcgcagaccg ccttgcagag catgttcgag
     2161 cgtactgcgg aagacaacgc cacgctgttg aagcaagtca gcttcttaga caagcgtgtg
     2221 actgtattgt cggagcagct gaagcagttc gggaacctct acaagcagaa caggcgttaa
     2281 gacggcagaa gaacgagaac gctcaagagc tgacaaagtc ggcaccaaaa atcgcccctg
     2341 tggcattcaa gaagagatca ggccctgatt atgggcctag catgtaatgc ctggatgaat
     2401 tgctctctgt gccttttaca attcatcttt ttgtaaaaaa ctaagaaata caggcaaaat
     2461 ttcttcttgc atgaaagctg cttccatttg agttgtcggt aaggaagccg ctcttttagc
     2521 gagtctcaaa caaactattt ttaacgcacc caaacaatct tctccttgaa taacaaggct
     2581 attttcagaa gaataatttt tcatgaaagt tagggttgaa tgcaatgcca ctgcttctgg
     2641 cataagccgt ttccctttgt gttttgcaaa aacatcgggc atggcacggt actgatttat
     2701 tattatgacg tgcgctaaat caggcacttg tagtttaaaa agattaggta aaaggcgaag
     2761 ctcagtatca acaatggttt ccactttaga aatataaaaa atttcagcaa tgccactgaa
     2821 catatcgccc tccggttaca gtcaaaacta tcagtattta ccaccttgaa taacgcttaa
     2881 accgtttgat agagcctttt tatgattact taccgtgcct ttccaaccaa actgcggatt
     2941 taaacggtag gttttgcttc gtccaatttt tggaccttca aggataatgc caaattcaat
     3001 caggttctta attgcgcggc ttacattagt tttctgcata cgcagagctt cagcaatgtc
     3061 aatctgtgca acctgaatat agttctcgta atccagttcg gcgagtagag ccattaacac
     3121 acgcatttga tcatggttta gctcgtttgc gatagtgatc agtgctgctt gattcatagt
     3181 gaaatgcctc tggaatgatg atttttgctt gggtcgtacc actgctacaa aaccacctaa
     3241 atcttctccg gtttcgagat ctacctgtgt gactctccgc atctgattct tcctgatagt
     3301 tatcttgaga gataactaag ttatcttgag agataactat tgtc
//
LOCUS       DAJSKJ010000254         3327 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000254 DAJSKJ010000000
VERSION     DAJSKJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.258"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ON345_003757"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ON345_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR3073736.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="ON345_003758"
     CDS             1828..3147
                     /locus_tag="ON345_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR3073737.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRKNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          830 a          822 c          802 g          873 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg acaatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttatctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaattg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taagtcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact agttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcggctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgcc gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatacggag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caaaaatact
     2641 cagcagttaa ttacaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaaccc tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccac agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagcc ataaatcccc taataaaaat
     3301 gtatgaatat gaaaaaacat acgatat
//
LOCUS       DAJSKJ010000255         3242 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000255 DAJSKJ010000000
VERSION     DAJSKJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.259"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            49..1200
                     /gene="yiaY"
                     /locus_tag="ON345_003759"
     CDS             49..1200
                     /gene="yiaY"
                     /locus_tag="ON345_003759"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR3073738.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1365..2903
                     /gene="aldB"
                     /locus_tag="ON345_003760"
     CDS             1365..2903
                     /gene="aldB"
                     /locus_tag="ON345_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR3073739.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT          785 a          814 c          884 g          759 t
ORIGIN      
        1 aatcctcagt aagctgccca ccctttttta cactttcagg agtgtgttat ggcatcttca
       61 actttcttta ttccttctgt gaatgtcatc ggcgctgatt cattgactga tgcaatgaat
      121 atgatggcag attatggatt tacccgtacc ttaattgtca ctgacagtat gttaacgaaa
      181 ttaggtatgg cgggcgatgt gcaaaaagca ctggaagaac gcaatatttt tagcgttatt
      241 tatgatggca cccaacctaa ccccaccacg gaaaacgtcg ccgcaggttt gaaattactt
      301 aaagagaata attgcgatag cgtgatctcc ttaggcggtg gttctccaca cgactgcgca
      361 aaaggtattg cgctggtggc agccaatggc ggcgatattc gcgattacga aggcgttgac
      421 cgctctgcaa aaccgcagct gccgatgatc gccatcaata ccacggcggg tacggcctct
      481 gaaatgaccc gtttctgcat catcactgac gaagcgcgtc atatcaaaat ggcgattgtt
      541 gataaacatg tcactccgct gctttctgtc aatgactcct ctctgatgat tggtatgccg
      601 aagtcactga ccgccgcaac gggtatggat gccttaacgc acgctatcga agcgtatgtt
      661 tctattgccg ccacgccgat cactgacgct tgtgcactga aagccgtgac catgattgcc
      721 gaaaacctgc cgttagccgt tgaagatggc agtaatgcga aagcgcgtga agcaatggct
      781 tatgcccagt tcctcgccgg tatggcgttc aataatgctt ctctgggtta tgttcatgcg
      841 atggcgcacc agctgggcgg tttctacaac ctgccacacg gtgtatgtaa cgccgttctg
      901 ctgccgcatg ttcaggtatt caacagcaaa gtcgccgctg cacgtctgcg tgactgtgcc
      961 gctgcaatgg gcgtgaacgt gacaggtaaa aacgacgcgg aaggtgctga agcctgcatt
     1021 aacgccatcc gtgaactggc gaagaaagtg gatatcccgg caggcctacg cgacctgaac
     1081 gtgaaagaag aagatttcgc ggttctggcg actaatgccc tgaaagatgc ctgtggtttt
     1141 actaacccga tccaggcaac tcacgaagaa attgtggcga tttatcgcgc agcgatgtaa
     1201 tcatcatttc cacaacggct ggcaaattgt cagccgcttt ttcaactatc tctgtaaccc
     1261 ttgcccgtaa attcgtgata gccgtcgtaa agctgttacc gactggcgaa gatttcgcca
     1321 gtcacgtcta cccttgttat acctcacacc gcaaggagac gatcatgacc aataatcccc
     1381 cttcagcaca gattaagccc ggcgagtatg gtttccccct caagttaaaa gcccgctatg
     1441 acaactttat tggcggcgaa tgggtagccc ctgccgacgg cgagtattac cagaatctga
     1501 cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc gggcaaacga gacatcgatc
     1561 tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc gcacacctcg gtgcaggatc
     1621 gcgcggcgat tctgtttaag attgccgatc gaatggaaca aaaccttgag ctgttagcga
     1681 cggctgaaac ctgggataac ggcaaaccca ttcgcgaaac cagtgctgcc gacgtgccgc
     1741 tggcgattga ccattttcgc tatttcgcct cgtgtattcg ggcgcaggaa ggtgggatca
     1801 gtgaagttga tagcgaaacc gtggcctatc atttccatga accgttaggc gtggtggggc
     1861 agattatccc gtggaacttc ccgctgctga tggcgagctg gaaaatggct cccgcgctgg
     1921 cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac cccgctttct gtactgctgc
     1981 taatggaaat cgtcggtgat ttactgccgc cgggcgtggt gaacgtggtc aacggcgcag
     2041 gtggggaaat tggcgaatat ctggcgacct cgaaacgcat cgccaaagtg gcgtttaccg
     2101 gctcaacgga agtgggccaa caaattatgc aatacgccac gcaaaacatt attccggtga
     2161 cgctggagct gggcggcaaa tcgccaaata tcttctttgc tgatgtgatg gatgaagaag
     2221 atgccttttt cgataaagcg ctggaaggct ttgcactgtt tgcctttaac cagggtgaag
     2281 tttgcacctg tccgagtcgt gccttagtgc aggagtcaat ctacgaacgc tttatggaac
     2341 gcgccatccg ccgtgtcgaa agcattcgca gcggtaaccc actcgacagc gtgacgcaaa
     2401 tgggcgcgca ggtttctcac gggcaactgg aaaccatcct caactacatt gatatcggta
     2461 aaaaagaggg cgcagacgtg ctcaccggcg ggcggcgcaa gctgctggaa ggtgaactga
     2521 aagacggcta ctacctcgaa ccgacgattc tgtttggtca gaacaatatg cgggtgttcc
     2581 aggaggagat ttttggcccg gtgctggcgg tgaccacctt caaaacgatg gaagaagcgc
     2641 tggagctggc gaacgatacg caatatggcc tgggcgcggg cgtctggagc cgcaacggta
     2701 atctggccta taagatgggg cgcggcatac aggctgggcg cgtgtggacc aactgttatc
     2761 acgcttaccc ggcacatgct gcgtttggtg gctacaaaca atcaggtatc ggtcgcgaaa
     2821 cccacaagat gatgctggag cattaccagc aaaccaagtg cctgctggtg agctactcgg
     2881 ataaaccgtt ggggctgttc tgatataaga agctggtcgc attgggtatt cattgcctga
     2941 tgcgacgctt acgcgtctta tcatgcctac gggaacctga ccgtaggccg gataaggcgt
     3001 acacgccgca tccggcataa acaaagcgca cgttgttaca atttacgttt taaacctacg
     3061 tgcgcaataa cccagactac gccaacagaa gtgcgtttct ggggcgtgtt ttgtcaggag
     3121 tttaatctcg cgtttcgatg gtgagcgggc gaaccaggcc gtaaatcacc attggtgatc
     3181 cgaggccaat cagcaataaa cggtagataa ttgaaggttt aatacgctta atctgtactg
     3241 tg
//
LOCUS       DAJSKJ010000256         3222 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000256 DAJSKJ010000000
VERSION     DAJSKJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.260"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..176
                     /locus_tag="ON345_003761"
     CDS             <1..176
                     /locus_tag="ON345_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3073740.1"
                     /translation="GDNFASGFIVALLEGKNLRECARFANATAAISVLSVGATTGVKN
                     RKLVEQLLEEYEG"
     gene            186..1166
                     /gene="ydjG"
                     /locus_tag="ON345_003762"
     CDS             186..1166
                     /gene="ydjG"
                     /locus_tag="ON345_003762"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR3073741.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALIINLSDADATLMREMAEALER"
     gene            1303..2061
                     /locus_tag="ON345_003763"
     CDS             1303..2061
                     /locus_tag="ON345_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3073742.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            2178..>3222
                     /locus_tag="ON345_003764"
     CDS             2178..>3222
                     /locus_tag="ON345_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073743.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTT"
BASE COUNT          874 a          642 c          799 g          907 t
ORIGIN      
        1 cgggcgataa ctttgcttca ggttttattg tggcactgtt agaaggcaaa aatctgcgtg
       61 aatgcgcacg ctttgccaat gcaacggcgg ctatctcggt tctaagcgtc ggtgccacca
      121 ccggcgtaaa aaacagaaag ctggtcgaac aattgctgga agaatacgaa ggataatgaa
      181 ggcaaatgaa aaagatacct ttgggcacaa cggatattac gctttcgcga atggggttgg
      241 ggacatgggc cattggcggc ggtcctgcat ggaatggcga tctcgatcgg caaatatgta
      301 ttgatacgat tcttgaagcc catcgttgcg gcattaatct gattgatact gcgccaggat
      361 ataactttgg caatagtgaa gttatcgtcg gtcaggcgtt aaaaaaactg ccccgtgaac
      421 aggttgtagt agaaaccaaa tgcggcattg tctgggaacg aaaaggaagt ttattcaaca
      481 aagttggcga tcggcagttg tataaaaacc tttccccgga atctatccgc gaagaggtag
      541 aagcgagctt gcaacgtctg ggtattgatt acatcgatat ctacatgacg cactggcagt
      601 cggtgccgcc attttttacg ccgatcgctg aaactgtcgc agtgcttaat gagttaaagg
      661 ctgaagggaa aattcgcgct ataggcgctg ctaacgtcga tgctgaccat atccgcgagt
      721 atctgcaata tggtgaactg gatattattc aggcgaaata cagtatcctc gaccgggcaa
      781 tggaaaacga actgctgccg ctatgtcgtg ataatggcat tgtggttcag gtttattccc
      841 cgctagagca gggattgttg accggcacca tcactcgtga ttacgttccg ggcggcgctc
      901 gggcaaataa agtctggttc cagcgtgaaa acatgctgaa agtgattgat atgcttgaac
      961 agtggcagcc actttgtgct cgttatcagt gcacaattcc cactctggca ctggcgtgga
     1021 tattaaaaca gagtgattta atctccattc ttagtggggc tactgcaccg gaacaggtac
     1081 gcgaaaatgt cgcggcactg attatcaact tatcggatgc agacgcaaca ttgatgaggg
     1141 aaatggcaga ggccctggag cgttaaatac tcaacacgat tcacctctgt ttctgtgaaa
     1201 actgtgatga aaatctgtga ttcaaacagg ttgttttgaa agtaaacatc ggttatgcga
     1261 taatcgcgct aatgtgatgt aaaaagatac aggggtgtaa tcgtggcggc aaaagacagg
     1321 attcaggcaa ttaagcaaat ggttgccaac gataaaaaag tgacagtctc aaatttgagt
     1381 gggatttttc aggtaaccga agaaaccatt cgccgcgatc ttgagaagct ggaagatgaa
     1441 ggctttttga ccagaaccta tggtggtgct gttttaaata cagcgatgtt gacggagaat
     1501 atccattttt ataagcgcgc ttcatcgttt tatgaagaga agcagcttat tgcacgcaag
     1561 gcactaccct ttatcgacaa taaaaccacc atggcagccg attccagtag taccgtaatg
     1621 gaattgctca aattgttaca ggaccgtagt gacctgacgt tgctaaccaa ctcggcggaa
     1681 gcgattcatg ttctggctca gtcagaaatt aaagtcgttt caacgggtgg ggaactaaac
     1741 aaaaatacac tttcactgca aggaagaatt actaaagaga tcatcagccg ctatcatgtc
     1801 gatatcatgg taatgagttg taaaggtctt gatattaaca gcggcgcgct ggactctaat
     1861 gaagcggaag ctgaaatcaa aaagacaatg atccgtcagg cgacagaagt tgcgttattg
     1921 gttgatcact ctaagtttga tcgcaaagct tttgtccagt tagctgattt tagtcatatt
     1981 aattacataa taactgataa atcaccgggt gcagagtgga tagcattttg caaagacaat
     2041 aatatccagc tggtgtggta atttaagcac acgttgtcaa tattatttgt tcctgcgcct
     2101 tgaaataaaa tgatgataaa gatgcggcaa gtgaatttgc cgcaattgct acctttttag
     2161 caaatccgag gcacctaatg gaacaatatg atcaaattgg cgcaagactg gaccgcttgc
     2221 ctttggcccg gtttcattat cgtatatttg gtattataag ctttagtctg ttattaacag
     2281 ggtttttgag ttactccggt aatgtcgtct tagcaaagct ggtaagcaat ggatggtcaa
     2341 ataatttcct caatgccgcc tttacctcgg cattaatgtt tggttatttc atcggctcac
     2401 ttactggtgg gtttattggt gactactttg ggcggcgcag ggcgtttcgc ataaatcttc
     2461 tcatcgtcgg tattgctgca acaggggccg cttttgtccc tgatatgtac tggctcatct
     2521 tctttcgctt cctgatggga acaggaatgg gggcgctgat tatggttggc tatgcctcat
     2581 ttacggagtt tatccccgcg acggtgcgtg gaaaatggtc cgcgcggctc tcatttgttg
     2641 gtaactggtc gcccatgctg tctgcggcga taggcgtggt ggttatcgct ttttttagtt
     2701 ggcgaataat gtttctgctg ggtggtattg gcatactgtt agcctggttt ctctcaggta
     2761 aatactttat cgagtcgcca cgatggctgg cagggaaagg gcaaatcgcc ggtgcagaaa
     2821 gccaacttcg tgaagtagag cagcaaattg aaagagagaa gagtattcgt ttacccccgc
     2881 ttacttcgta tcagagcaac agcaaggtta aagtaatcaa gggtactttc tggctcctgt
     2941 ttaaaggtga aatgttacga cgtacattag tcgcgattac tgttttaatt gcaatgaaca
     3001 tctcgcttta taccatcacc gtatggatac cgaccatatt tgttaactcc ggcattgatg
     3061 tcgataaatc aatattaatg accgctgtta ttatgattgg cgctccggta ggaatattta
     3121 ttgcggcatt aattattgat cattttcctc gtcggttatt tggctccacc ttacttatta
     3181 ttattgccgt gttaggctat atctattcaa ttcagactac ag
//
LOCUS       DAJSKJ010000257         3213 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000257 DAJSKJ010000000
VERSION     DAJSKJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.261"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ON345_003765"
     CDS             complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ON345_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR3073744.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSE"
     gene            complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ON345_003766"
     CDS             complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ON345_003766"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR3073745.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1699..1968)
                     /locus_tag="ON345_003767"
     CDS             complement(1699..1968)
                     /locus_tag="ON345_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR3073746.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2038..2622
                     /gene="mobA"
                     /locus_tag="ON345_003768"
     CDS             2038..2622
                     /gene="mobA"
                     /locus_tag="ON345_003768"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR3073747.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2604..3131
                     /gene="mobB"
                     /locus_tag="ON345_003769"
     CDS             2604..3131
                     /gene="mobB"
                     /locus_tag="ON345_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR3073748.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          834 a          786 c          784 g          809 t
ORIGIN      
        1 tctcggacag atatttcact gtatcagcat actgctgaac aaaaacatcc atattgctgg
       61 tatccatacc ctgcggattc agctgatatt taccgttaac aaacatcgcc ggaacgccac
      121 gcaattgcac gtcagctgca gctttttcct gctgagcgac cagagatttg accacgaagc
      181 tgttccacgc cgcgtcgtac tcttcacctt taatacctgc gttgataaat acatcgcgga
      241 tatcagatgc tgaacgaatg gtctgggttt tctgtacgcc ttcaaacagc ggaacagtga
      301 ctttgtcttc cacgcccagc gccatcgcca cagcccatgc ctgagtcaga tctttgccca
      361 ggtcaccacc catgaagttg acgtggtatt tagtcatctt cacgccttcc ggcagttttt
      421 tcttcacatt atcagaaata tgcagaactt cttcaaactg atagcagtgc ggacagaaga
      481 aagagaaaaa ctccagcact tgcggcgcgc cagctaccgg tttttccagg gtagtgtact
      541 gtttaccatc ttcatactgc gccgccgatg cgctaaacgc taaaactaaa ccagccagcg
      601 ccagccaaat ctttttcatg atctactctc tccgattaat acataggtgt taattgtaaa
      661 gggggttctt gtagaacttt tgcctgttct ataaaagtcg ccgtttgtcg cagccagtaa
      721 tcttccccgg ttaaccacgg gaaatttttc gggaacgcgg gatcagccca acgccgcatt
      781 agccaggcaa gataataaac caaacgcatg gcgcgtaaag gttcaatcag tccgatttca
      841 gcggtgtcga actcgctaaa ttcttcataa gcttcaataa tagtttccag ttgcatccgc
      901 tgctcggctt tatcgccatt gagcaacatc cacaaatcct gaatagctgg accattacgt
      961 gcatcatcca gatcaacaaa cattggacca tcgcgccaga gaatattccc ggcgtggcag
     1021 tctccatgta gccgcagaac ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc
     1081 agctcatctg tcgctttcag gaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc
     1141 tcaaacagct tgcgtggctc aatgagatat tcattcagac cgatggtcgg gcgatggata
     1201 aaaagctgtt tgtgccccgt ctggtgcata cgccctaaat aacgcccaac cgcttccgtc
     1261 tgatcgatat tatcagcttc gaactggcga ccaccgacgc ttggaaaaac agcgaaataa
     1321 aatccctgat gattcaataa agtctgaccg ttaaaggcca caggtgctgc gaccggaact
     1381 tcatcattta ccagctgcaa cgcaaattga tgttcttcga ggatttgatc ggctgtccaa
     1441 cgttcagggc gataaaattt gacgacaaaa cgtcgacgct cttcgtcctg aaattgatag
     1501 acacggtttt catagctgtt aagcggggta agaccggaat ccacccggat cccttgctca
     1561 aacagagcgt ccatgatggt atccgggtgt agtgtctgga aagtaaaagc gctgttgttc
     1621 atcccatcat ccggaaaaca ttacgaatga tgaaggatat catttcacga tgattttggt
     1681 ggcgccgctt acaagctttt actctttaat tacgccgcga gcgcgtaaca gcgccgtttt
     1741 gaagtcttct tcataatctt tctgcaaacc cgggatcacc gcatctttcg cggaatcacg
     1801 cattttcagg tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc
     1861 tgactctttc gccagtttct gcaaaaattg caacaggtta aggtctggct ctttttgcca
     1921 ggctggctgg aggagttcaa taacttcatt cagacgttta catttcatag tgatgctcct
     1981 tactcttgag acagacacgt tagcagggtc aatcccacaa taaaagaggc gatatcggtg
     2041 aatctgatga cgacgataac aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc
     2101 ggcgtagata aaggattgct ggaattaaat ggcaaaccgc tgtggcaaca tgtcgctgac
     2161 gcgcttatgg cgcagctctc tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat
     2221 caggcaagcg gtctgaaagt gattgaagat tcactggcgg attacccagg ccctctggca
     2281 ggaatgcttt cagtaatgca gcaggaagca ggcgagtggt ttttgttttg tccgtgcgat
     2341 actccttata ttcccgagga tttagcagcc cggcttaatc atcagcgcaa agatgcgcct
     2401 gtcgtgtggg tccatgacgg tgaacgcgat cacccgacta ttgctctggt aaaccgcgct
     2461 attgagcctt tattactgga atatctgcaa gcaggagaac gccgggtaat ggcatttatg
     2521 cgtctggctg gcggtcatgc ggttgatttc agcgatcata aagatgcatt tgttaacgtg
     2581 aatacgccag aggagcttgc ccgatggcag gaaaaacgat gataccgtta ctcgcctttg
     2641 ccgcgtggag tggtaccgga aaaactacgc ttttgaaaaa actgatcccg gcattatgcg
     2701 ccagagggat ccgtccaggg ctgattaagc atacgcacca tgatatggat gttgataagc
     2761 caggcaaaga tagctatgag ctgcgcaagg ctggcgcggc acaaaccatc gttgccagcc
     2821 agcaacgatg ggccttgatg acggaaacac cagacgaaga agagctggat ctacagtttc
     2881 tcgcaagtcg aatggatacc tcaaagctgg atttgattct ggtcgaaggg tttaagcatg
     2941 aagagatcgc aaagattgtg ctgtttcgcg atggagccgg acatcgacct gaagaattag
     3001 tgatagacag gcatgttatt gctgttgcca gtgatgtgcc gcttaatctt gatgtcgcat
     3061 tactggatat taatgatgtt gaggggctgg ctgattttgt ggtggagtgg atgcaaaagc
     3121 agaacggata agcaagagta ggctggataa gacgcgccag cgtcgcatcc ggcatttttc
     3181 tgcaccagcg caaaaaggcc cagtcttccg acc
//
LOCUS       DAJSKJ010000258         3196 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000258 DAJSKJ010000000
VERSION     DAJSKJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.262"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..73)
                     /locus_tag="ON345_003770"
     CDS             complement(<1..73)
                     /locus_tag="ON345_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3073749.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIV"
     gene            complement(85..315)
                     /locus_tag="ON345_003771"
     CDS             complement(85..315)
                     /locus_tag="ON345_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR3073750.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            496..2127
                     /gene="dcuS"
                     /locus_tag="ON345_003772"
     CDS             496..2127
                     /gene="dcuS"
                     /locus_tag="ON345_003772"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR3073751.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2124..2843
                     /gene="dcuR"
                     /locus_tag="ON345_003773"
     CDS             2124..2843
                     /gene="dcuR"
                     /locus_tag="ON345_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR3073752.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEMIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDTHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT          885 a          789 c          730 g          792 t
ORIGIN      
        1 agacaatttc agcgatttgc ttgccttgtt cgtcattaat gtaaaattta ttgtggcctt
       61 cgcgtatttc cattttaccc tcgcttattt atgacggtat ttcagcgcac cactcgggca
      121 cgtatcaatc actttcacca cagtggcaac gtcgacttca tccggcatga tccacggctt
      181 tcgtttgaga ttaaataact tgccgttgcc acgtacgcaa ttgccagaat gctgacatat
      241 cgcagtgttg aaatagacat cgatcttttc gccggtataa cagcgataac cctcgtccag
      301 tagcgcctga tccatgactt tccctctctc atcagtatga ttatcgctat aagcatagcc
      361 ctggaatctt catacagcac tggccgtttc cgttgccaga gtctcttccc ctatatagcc
      421 aatcattctt actccctttg aattacccgc ctcatcagag ataatgctta agaaatttgt
      481 cacacaagga agctgatgag acattcattg ccctaccgca tgttacgcaa acgtccgatg
      541 aaattgagta ccacagtgat cttaatggtc agtgcggtac tgttctcggt gctattggtg
      601 gtgcatctga tttacttctc gcaaatcagt gatatgacgc gagacgggct agccaacaag
      661 gcactggcag tggcgcgtac cctcgccgac tcgccggaaa tccgtcaggg cttgcagaaa
      721 aaaccgcagg agagcggcat ccaggccatc gcggaagccg tgcgcaaacg caacgatctg
      781 ctgtttattg tcgttaccga tatgcaaagt cttcgctact cgcatcctga agcccagcgt
      841 attggtcagc catttaaagg tgatgacatc cttaaagcgc tgaatggcaa agaaaatgtc
      901 gctatcaatc gcggttttct ggcgcaggct ttacgcgtat ttacccccat ctacgatgaa
      961 aatcataaac aaattggcgt ggtggcgatc ggccttgagt taagccgcgt gacccaacaa
     1021 atcaatgaca gtcgctggag cattatctgg tcggtattat ttggcatgct ggtcggactg
     1081 attggcacct gcattctggt taaggtactg aaaaaaatcc ttttcggcct ggaaccctac
     1141 gaaatctcca cgctgtttga gcaacgccag gccatgttgc agtctatcaa agaaggcgtc
     1201 gttgccgtgg acgatcgcgg cgaggtcacg ctgatcaacg atgccgcaca agaattgctg
     1261 aattaccgta agtcgcagga cgatgagaaa ctgtcgacgc tcagccactc atggtcacag
     1321 gtggtagatg tctcggaagt gttacgcgac ggtacgccgc gccgcgacga agagattacg
     1381 attaaagacc ggctattact gatcaacacc gttccggtgc gcagtaatgg cgttatcatc
     1441 ggtgccattt caaccttcag ggacaaaacc gaagtacgta aactgatgca gcgactcgac
     1501 ggtctggtca actatgccga cgcacttcgt gaacgatccc acgaatttat gaataaattg
     1561 cacgtgattc tcggattatt gcatctgaag agttataagc agttggaaga ttacattctc
     1621 aaaacagcca ataactatca ggaagagatt ggctctctgc tgggtaagat caaatctccg
     1681 gttatcgctg gttttttaat cagcaagatt aaccgcgcga ccgatttagg ccatacgctg
     1741 attttaaaca gtgaaagcca gctgccagac agcggcagtg aggaccaggt cgcgacgctg
     1801 attaccacat tgggaaatct gatagaaaac gcgctggaag cattagggcc ggaacccggt
     1861 ggcgaaatta gcgtaacatt gcactaccgt cacggctggc tgcactgcga agttaacgat
     1921 gatggaccgg ggatcgcacc cgacaaaatc gatcacattt ttgacaaagg tgtctcgaca
     1981 aaaggaagcg agcgaggcgt cggtttagca cttgtcaaac aacaggtaga aaatctcggc
     2041 ggcagcatcg ccgtggaatc ggaacccggg attttcacac aattttttgt ccagataccc
     2101 tgggacgggg agaggtcgaa cagatgatca atgtattaat tatcgatgac gacgcaatgg
     2161 tcgcggagct gaatcgccga tacgtagcac aaatcccagg ctttcaatgc tgtggaacag
     2221 cctcgacgct ggagaaagcc aaagagatga tcttcaatag cgatacgcct atcgacctga
     2281 tattgctcga tatctatatg caaaaagaga acgggctcga tttactgcct gtcctgcata
     2341 acgcgcgttg caaaagtgat gtgattgtca tctcctccgc agccgatgcg gcaaccatta
     2401 aagattcgct gcattacggt gtcgtggatt acctgatcaa acccttccag gcttcccgct
     2461 ttgaagaggc gctcaccggc tggcggcaaa agaaaatggc gctggaaaaa catcagtatt
     2521 atgaccaggc cgagcttgat cagctaatcc acggcagcag ctccaacgaa caggatcctc
     2581 gtcgcttgcc aaaaggctta acgccgcaga cgttacgcac actgtgccag tggattgaca
     2641 cgcatcagga ctatgagttt tcaaccgacg agctggctaa cgaggttaac atctcgcgtg
     2701 tttcctgccg aaaatacctc atctggctgg tcaactgcca catcttgttc accagtatcc
     2761 attatggcgt cacggggcgt ccggtttacc gttaccgtat tcaggcagaa cactactcac
     2821 tgctgaaaca atattgccaa taacggatcg ctggttatct gtaagtaata attattaccg
     2881 taatgcaaaa tacccctggc acaagttaca tccttcatca gtaaaacttg tgccagatca
     2941 aatataatta tccctccatc atcgctaaaa attaatatct cttcaggtga acggtgtttt
     3001 taatttcaaa acgctaacaa aagttaatta actattatgt cacccgcatt atgtgtattt
     3061 ttacccacaa atgggtagat cagattaatc tttaaaccta atgatatctg ccctgagaac
     3121 aaaaaataga ccgataaata tcaataagat aacagcaaac aaaacattaa catctgcgca
     3181 gtacaaacta taaacc
//
LOCUS       DAJSKJ010000259         3178 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000259 DAJSKJ010000000
VERSION     DAJSKJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.263"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..887)
                     /gene="intS"
                     /locus_tag="ON345_003774"
     CDS             complement(<1..887)
                     /gene="intS"
                     /locus_tag="ON345_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR3073753.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATGLAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGR"
     gene            complement(1049..1123)
                     /locus_tag="ON345_003775"
     tRNA            complement(1049..1123)
                     /locus_tag="ON345_003775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Arg,seq:cct)
     gene            complement(1199..2131)
                     /locus_tag="ON345_003776"
     CDS             complement(1199..2131)
                     /locus_tag="ON345_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR3073754.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNNPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2425..>3178
                     /gene="mlaA"
                     /locus_tag="ON345_003777"
     CDS             2425..>3178
                     /gene="mlaA"
                     /locus_tag="ON345_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR3073755.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          739 a          775 c          777 g          887 t
ORIGIN      
        1 cgcccggcaa aaacaaactc tgatactggt ttggtgattg agcttagtgt agtgagaagt
       61 tcaaccacct ggtctgacat aggaaccaca tgaattttgc gtcccttcat cacactggcg
      121 tcgatggtga taatcctgtt ttcaaaatcg acgttcttcc attgcatgga acgaagctct
      181 ttcgttctta gggctgtgta gcgtaaaacc ttggtggcaa tgagcgatac gatacttcct
      241 gaaaatgttg ccagtgcttt gttgaatgcc gggatctggt ctgcaggaag aaacgggaag
      301 ttcttcttgc ggtatccctt catggcgtca gcaaggtcag gtgccgggtt atatttagcc
      361 cttccggtga caatagcgta acggaaaacc tctccgcatc ttctgcgggc tttgttggct
      421 cgctccattg caccgcgatc ttcaaatctg cggattactt ccagcagttg catcggctca
      481 atatcctgaa tctcaagacc gccgatgatg ggtaaaatgt cgtcatcaaa cattttggca
      541 agtccagttg catagcctac tgaccagact tgcttcttgt gctcgtacca ttccttgtaa
      601 atggcactaa aggaattgtt gttagacgaa gcctttttcg cttttaccgg atcgatgcca
      661 accgagatgt ctttcctcgc ggtccatgct ttatctcttg cctcctgcaa agtcattagc
      721 ggatattttc ctacggtcag gattttctcc ttaccgtcaa tcttgtagcg aagctgccat
      781 acctttttcc ctgacacagg gacataaagg tacaggccat taccatcgag taggcggtat
      841 ggtttttctt tcggctttgc tgcttcaatc tgcttaacgg tgagcatggg taaaaatccg
      901 gtgggtaaaa ttattttatc cactttttac ccgtcatgga gtgcggctgt caacgatctg
      961 acgcgaacca ttacgaactg tgaatctgcg gaaggcttga tattcaggga attttgcgga
     1021 ctggtacgga tgggagcgaa ctgataaatg gtgtcccctg caggaatcga acctgcaatt
     1081 agcccttagg aggggctcgt tatatccatt taactaagag gacaatgcgg catgagtata
     1141 cccgctaatg gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt
     1201 atgctgggtt tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt
     1261 ctgctttacg cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga
     1321 taaaggtgcc gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat
     1381 cgctccagtg cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga
     1441 tatgggtggt gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg
     1501 ccgcacctgc tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg
     1561 cgttggcaaa catctcgctg gggttgttct tcatcacatc catgccgatt ttgacaaatg
     1621 catcgcgagt ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag
     1681 tcccgagaat attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc
     1741 tcattgtcgg tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt
     1801 gctggcgggc cattatgacg ataataaaac caaaggtata accgagattc tccagtaaga
     1861 agctgcccgg cacaccttcc agttcgacat gaaatatccc ttttgccagt aacgaagcgc
     1921 ccatcgacag acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttccagct
     1981 ctttttcacc atcctggcgg atatgctcat gaattgccat cgcccgggag gggagtcggt
     2041 cttcatctat ttctattttt ttgccgcgct ctttttcttc gctctcaact tcaatttcgt
     2101 cgctgtgttg atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag
     2161 ctaaagcgta gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa
     2221 ggctgcgttt gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc
     2281 aggctatgat caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat
     2341 cgcccacacc taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag
     2401 ccataaaaaa acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact
     2461 acgcttctgg tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta
     2521 gaagggttca accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt
     2581 cgaccggtcg ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc
     2641 aactttactg gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac
     2701 ccttatcagg ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc
     2761 ggctttattg atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc
     2821 ttcggtagta cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc
     2881 tacggtagct tcacgctgcg tgatgacggt ggtgatatgg cggatggtct ttacccggtt
     2941 ctttcctggc tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc
     3001 cgcgctcagc tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg
     3061 gtgcgcgaag cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg
     3121 caggaaaatc cgaacgcaca agcgattcag gatgatttaa aagatattga ttctgaat
//
LOCUS       DAJSKJ010000260         3175 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000260 DAJSKJ010000000
VERSION     DAJSKJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.264"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(48..809)
                     /gene="kduD"
                     /locus_tag="ON345_003778"
     CDS             complement(48..809)
                     /gene="kduD"
                     /locus_tag="ON345_003778"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR3073756.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(839..1675)
                     /gene="kduI"
                     /locus_tag="ON345_003779"
     CDS             complement(839..1675)
                     /gene="kduI"
                     /locus_tag="ON345_003779"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR3073757.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1962..3143)
                     /locus_tag="ON345_003780"
     CDS             complement(1962..3143)
                     /locus_tag="ON345_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR3073758.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          825 a          877 c          723 g          750 t
ORIGIN      
        1 taaaaaaccc tgccatgcgg cagggtcata aaagtaagaa gaatgaatta acgcgccagc
       61 caaccgccat ccacggcaat ggtataacca ttcacataat ctgaagcgct ggaggcaagg
      121 aacactaccg gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcggtcg
      181 aggatttccg cgctacgttg ttcatctgcc cgtagttgtt gagtattgtt ggtcgccatg
      241 taacccgggg ctatcgcatt aacattaatg ttgtgtttag cccattcgtt cgccatcaat
      301 cgcgtcacac ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc
      361 tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag
      421 tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg
      481 tcccagtctt tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc
      541 accaggatat caatatgacc aaactccgct accgcgcgat ccagcagtgc tggaatgcca
      601 tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc cgtgacctgc
      661 ttgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc
      721 agccccaacg ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta
      781 ccttcgagag aaaatgcact taaaatcata acaatacctt attcgtttat gcccacaact
      841 agcgcaaatc tttaacggcc acatggtcca tatcatcaaa gacctggttt tcaccgacca
      901 tgccccagat aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg
      961 agatcaccgc ctgctcgtta tgcattacaa tatgacgcgt ttcttgcggc tgccccatca
     1021 tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct
     1081 cgtgagtgtg acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca
     1141 tactcaattg gcaggtttcc agcacatccg ggacaaaata tttgttaatt gtgcgacggt
     1201 tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg
     1261 tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa ctttgccgga gtggcggtat
     1321 caatactggc aaagacaact tcttttgcac ctttacccac atacagggcg tcgcggtgac
     1381 cgatttcata gcattggcca tcgacagtaa tcgtaccggc accgccaata ttgataacac
     1441 ctaactcgcg acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa
     1501 cggaaaccgt tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt
     1561 aaaccatggt gtactcatcg gcgacaaata cctcttcaac caaaaattca ttgcgcagcc
     1621 cttgggtatc cagcgttttt gcgtgcgcac tgtggatgct ctgtcttacg tccacatcaa
     1681 cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgctttc
     1741 tctataaagg gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt
     1801 catttttaag ttaaggatta taaaaagtgc agtgtcgatc acgaatatgt catgagaatg
     1861 gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat
     1921 taatgacggc agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg
     1981 gtcaatgcca cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta
     2041 cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg
     2101 tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc
     2161 ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca
     2221 gccaactgcc agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc
     2281 atcaatgccg gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa
     2341 ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg
     2401 gatgatgcat tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct
     2461 aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga
     2521 ccgttacttt gggtcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc
     2581 gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaattgacg gctgatgcca
     2641 tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccat
     2701 aacccatcat gcacaagact gtcaaccagc tggctattgc caagctgtgc accggtgcgg
     2761 ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata
     2821 tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag tccggaaccg
     2881 cagacgtcat taatcgtgat tgcagaaacg ctattaggca gaccaccttt aatagccgat
     2941 tgccttgccg gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc
     3001 accgcatatg caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt
     3061 tccacggcgg aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc
     3121 ccgacaatca caacgtcttt catcacaacc tcgcaccaat taacagtgca atagt
//
LOCUS       DAJSKJ010000261         3100 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000261 DAJSKJ010000000
VERSION     DAJSKJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.265"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..939)
                     /locus_tag="ON345_003781"
     CDS             complement(<1..939)
                     /locus_tag="ON345_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HCR3073759.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVY"
     gene            1002..1205
                     /locus_tag="ON345_003782"
                     /pseudo
     CDS             1002..1205
                     /locus_tag="ON345_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            1163..1840
                     /gene="bglJ"
                     /locus_tag="ON345_003783"
     CDS             1163..1840
                     /gene="bglJ"
                     /locus_tag="ON345_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR3073760.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRLSFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1878..2666)
                     /gene="fhuF"
                     /locus_tag="ON345_003784"
     CDS             complement(1878..2666)
                     /gene="fhuF"
                     /locus_tag="ON345_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR3073761.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLVVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2807..3043
                     /locus_tag="ON345_003785"
     CDS             2807..3043
                     /locus_tag="ON345_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR3073762.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          772 a          698 c          770 g          860 t
ORIGIN      
        1 gtaaacttta acgccggtgc tgacagtaaa ttctagatgt ctggccagtt ccattcctct
       61 gtcccatgtc agtgattttc tgagttctga cggtaaactc aggaatttgt cggtaagagc
      121 ctgatttact gagacagaat ctttgcccct gagtctaagg atgatcgtat aacgtgattt
      181 tcggtctaca agtgtggcta tatgagagtt ttttgtacct gagactaaat cgccctccca
      241 atgccccaga gagcgtctgt tatcgatatt tcgggaacgt tcgtgaattg gtgttccgtt
      301 cactatgtta atcgtacctc tttcgccttt gcgggtatga cgcctgccat ggcgaaggct
      361 atgcgaccgt cgcagatgct gtatattcag gtggtgtagc gcttcacggc tacgaaagta
      421 cagcgtttta taaattgtct caggtgatat tcgcagcgtt ttttgacgtg gttttgttcg
      481 ccttaaccat cctgatattt gctctggaga ccatttcatc tccagctttt ccagaacaag
      541 ctttcgcaat ggtaaatttt gatccagtaa gcacggtttt ggccttttcg ccattctgtt
      601 ggctcggtta ttagcatcaa cagctttgta atagcgtctg ccccgattac gctgaacttc
      661 acgtgagatc gtcgaaggac tgcgattcag cgcagtagct atcgcacgaa tgctcatttt
      721 ggctgacaaa ccagctcgta tctcctcgcg ctcagacagt gtcaggtgag ctacagcccg
      781 cttacgctca tggggtttta tgccgccagt atcccttaac atagtgaaga tcgttccggg
      841 ttttgaaccc aggatattcg ctatttcact gaagcctgtt ccgttcttcc atagttcaaa
      901 aacagaggct ttttcctctg ctgtaaatgt tcgtctcatt caaaaaacct ccgcaacccc
      961 atgttttcac ataactgttg cgttgaccaa ttgaatctac agcttcgttg cttaagaaaa
     1021 gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg tcgactggca attcacagca
     1081 acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc aagagaactt aacttgcctt
     1141 ctgtttatgg agatgccgca gaatggaaca cagccgaatt aagaagagaa atgtcgcact
     1201 catagaaaaa tgcgtcatga gcagtatcgg tattgagagt ttattcagaa agtttgcggg
     1261 taacccttat aagctccata cctataccag tcaggagtca tttcaggatg ccatgtcgcg
     1321 gctctcgttt gcggcggtca ttttttcttt ttctgccatg agaagtgagc gcagagaggg
     1381 attatcttgc ctgactgaac tggcgattaa gtttccgcgt acccggcgtt tagttattgc
     1441 ggatgatgat attgaagctc ggctgattgg ttcattgtcg ccatcaccgc tggacggtgt
     1501 attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa ctctttttgt cattaaatgg
     1561 tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac attaaccaaa gccggacgtt
     1621 aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt ggctactcaa tgacacaaat
     1681 tgccgagcag cttaaacgca atatcaaaac gatccgtgca cataaattta atgtgatgtc
     1741 gaaactgggc gtcagttctg atgcagggtt gttggaggcc gcagatattc tgttatgtat
     1801 gcggcattgc gaaacaagta atgtgttgca tccctattaa tccgcatgat gccgggttta
     1861 cttccccggc agtgctttca tttcagcgta caatcgccac attgctgcac atccggtaag
     1921 cgataacgct ggcagcaagt gcggcgcacc agcaggccat cgcgcagtac cacggtacgc
     1981 cagagtggat tatcttcacc gttcgtgagc gttttctcaa aaaagagggc atggcgcagc
     2041 gattcaaccg tcgcctcgcc gagcagttgc ttcatctcag taagatacca gttgatcaaa
     2101 taaccggtat tactccagat aagtttgccg ttgatctctc cggtcgcttc tagtgcctgc
     2161 acaaccggaa ccagcgcctg gctgattaac ctttccattc gctgctgcgg cgaatgtggt
     2221 gttgcgtttt tatcttcaca cacatcgacc cagaaacagg cggcacggcc ggtttcgtga
     2281 aactcagcat ggaaatgttc cggcgacaca tctaatgcct tttcctgcgt cagtagcgcc
     2341 agcattaatg gtggcaccat caggccgata taccattgtg cccatagtga gatcagcggt
     2401 ttgttctcgc ggatcatcgt tggttggttg cgatagatat gatcggaata gaccacgagt
     2461 agagaactta gcgcattcgg tgatgaccat tgcgccagcg tcatggcgtt aagcggagcg
     2521 ggttcatcca ggcggataaa ctccagcaaa tgttcacgat gttcggcgat cgtcgcacgt
     2581 acggcttgtg caagcccagc atcctgtggc tggagatgcg ttcgccagat aacatcttca
     2641 tagagcggtg cggaacgata ggccataatc gggatagtaa tctaaatgat aatgattgct
     2701 aatcctagcg ataggtttac ccgatagcaa gggatttctc tggcttgcaa atgataaaaa
     2761 ttatcatatg atattggtta tcattatcaa tgaaagagat gaaaccatgt tgcaacgtac
     2821 gctgggcagt ggctggggag tgttgctgcc aggattgctg attgcagggc tgatgtatgc
     2881 ggatttatca ccggatcagt ggcggattgt cattctgatg ggattagtat tgacgtcggt
     2941 gatgctgtat cacaaacagt tacggcatta cgttttgcta ccatcgtgcc tggcgcttat
     3001 tgctgccatc atgctgatga taatgaattt gaatcaggga tgaaaaatca aggaagaaac
     3061 aagaaaggaa gtaaagataa ttggtgcgag gggggggact
//
LOCUS       DAJSKJ010000262         3081 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000262 DAJSKJ010000000
VERSION     DAJSKJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.266"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..163)
                     /locus_tag="ON345_003786"
     CDS             complement(<1..163)
                     /locus_tag="ON345_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HCR3073763.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTF"
     gene            405..698
                     /locus_tag="ON345_003787"
     CDS             405..698
                     /locus_tag="ON345_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR3073764.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            770..1429
                     /locus_tag="ON345_003788"
     CDS             770..1429
                     /locus_tag="ON345_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR3073765.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1521..1967
                     /locus_tag="ON345_003789"
     CDS             1521..1967
                     /locus_tag="ON345_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR3073766.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2174..3076)
                     /gene="hlyE"
                     /locus_tag="ON345_003790"
     CDS             complement(2174..3076)
                     /gene="hlyE"
                     /locus_tag="ON345_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131199601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR3073767.1"
                     /translation="MVADKTVEVVKNAIETADGALDLYNKHLDQVIPWQTFDETIKEL
                     SRFKQEYSQAASVLVGDIKTLLMDSQDNYFEATQTVYEWCGVATQLLAAYILLFDEYN
                     EKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKS
                     SYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSITAGVVEGKLIPELKNKLKSVQNF
                     FTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAK
                     KMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT          799 a          662 c          734 g          886 t
ORIGIN      
        1 agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc ggagtactga gcgctgctat
       61 ggccttttct gaactcaaca ttgacattct tacccgcagc aaagctcgtg gaggttagca
      121 gtgcaaacag caatacagcc ttactgatgc tcttgatttt cattggtagt ctcctgttca
      181 gaatgagtac gatgaactgt tataatataa caatccctta cgggagaatt ccagtggctt
      241 ttctgcgttc ggtaaaatcc cggcgcggca ttttttgccg gggggattac ttataaaggg
      301 gcatggactc aacaaaccgc agaaatctac agctggctgg cttgtggcgc gggtttcatt
      361 gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt tgtgtgattt
      421 atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac gatttttcgc
      481 gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg attctgccgt
      541 tggatgggcg gaagaaactg gtcaatgctg atattgaaaa ggttaaactg gcattaaccg
      601 agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag caacatcttt
      661 ccgtcatggg gcagaaaaca gacgacacta acaaataacc gatatccggc ggtggcatta
      721 tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta tgtatcaaca
      781 tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagtct gtgttggcag
      841 taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc cagtgctgtt
      901 tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc catccgattt
      961 cggttcagtt catcatgaag tcgaactggc ggtgttgatt ggcgcgacgc tgcgtcaggc
     1021 aacggaagag catgtccgca aagccattgc cggttacggc gtggcgcttg atctgacatt
     1081 gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg ctaaagcgtt
     1141 tgataactct tgtccgcttt ccgggtttat ccccgcggcg gaatttaccg gcgatccaca
     1201 aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta cgactgcgga
     1261 catgatccat aaaatcgttc cgttgatcgc ttatatgagc aagtttttta ccctcaaggc
     1321 cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc ccgttgcaaa gcggtgatga
     1381 gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat actttttgcc
     1441 gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag gtgcgtttta
     1501 acgtaatggc ggaacacctg atgagcgatg tacctttctg gcaaagtaaa accctggacg
     1561 aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata
     1621 aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt cgccagctca
     1681 atattaaaac ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa cccgactgca
     1741 ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct gccaatgacc tgcgcttatc
     1801 ggttgctggc ggaaggtaaa gatttacctg cgtggcatcc gctacttact ggttcgaaag
     1861 cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga
     1921 ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa atttaataga
     1981 tgctgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag ccctgttttt
     2041 gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacagg taactctcaa
     2101 tctttaaaaa acgtctgtgt ttttaaatgt gataaacctt ataccttgag tacatggaga
     2161 gaataatcgc ttatcagact tcaggtacct caaagagtgt ctttttaccg tgtcttttct
     2221 gatactcatt acaggtgtta atcatttttt tggccgcttc ttttagcaaa gaaagcatta
     2281 aatcatcata atcaacatag aatctggttg tttcagtttc cgtttttatc tctccgatgg
     2341 cggctatttc ggtggttaat ttcaatttgg cggcatcgat atctttattc gcttgtttaa
     2401 ccgtgttaga cagggtggta aagaaattct gcacagattt taacttgttc ttcaattctg
     2461 gaatcagttt tccttcaact acgcccgcag taatagaata ggaaatgatt aatccaaatg
     2521 gaccggcgac gacaccggct gcggcaccgg catacgcttc cttcctgatt ttatctacct
     2581 gtgactggaa atagctgctt ttttctgaaa aatcattggt taactggcta tctaacgcca
     2641 gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac cagcagggat ttttgcgctt
     2701 cattcagctt cgtgatgccg tcatccagta ccttaatgag aatgtctttc tgggcggatg
     2761 ctttcttctc attgtactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa
     2821 caccacacca ttcatacact gtttgggttg cttcaaaata attatcctgg ctatccataa
     2881 gtaaggtttt aatatcgccg actaaaacgg aggctgcctg tgaatactcc tgtttaaagc
     2941 gacttaactc ttttatggtt tcatcaaagg tctgccaggg gatgacctga tcgagatgtt
     3001 tattataaag atctaatgct ccatctgcgg tttcgattgc gtttttaact acttctaccg
     3061 ttttatctgc aacgatttca g
//
LOCUS       DAJSKJ010000263         3040 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000263 DAJSKJ010000000
VERSION     DAJSKJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.267"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(41..1342)
                     /locus_tag="ON345_003791"
     CDS             complement(41..1342)
                     /locus_tag="ON345_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR3073768.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(1342..1845)
                     /locus_tag="ON345_003792"
     CDS             complement(1342..1845)
                     /locus_tag="ON345_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR3073769.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(1890..2876)
                     /locus_tag="ON345_003793"
     CDS             complement(1890..2876)
                     /locus_tag="ON345_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR3073770.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGDARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          934 a          688 c          637 g          781 t
ORIGIN      
        1 tttgccggat aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct
       61 gtggaatgaa caaggatatt tgcggaatgt aagtgatcat catcaacgct actagcaatg
      121 cagcataaaa tggcagcaat ggtttgatta aatgttgaat cttcacaccc gagatagaac
      181 aaccaacaaa tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttggcca
      241 ccatcataat accgaaatgt actgggtcca ttcccagctc ttgtgcaatc ggcaagaaga
      301 tcggtgtaaa gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga
      361 cgatattaat cagcagcaat atgatcagtg ggttatcaga aatccccatc aatgcgtcgc
      421 tgatcatata aggaatatca gcattagtca ttgcccagga catgcccacg gaaaaaccga
      481 tcaacagtaa tacaatcgaa gtggtaacaa ccgactccag aatcagtttg ggtaaatggc
      541 gccattttac ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg
      601 ctgacgcttc agttgcggta aaaatgccgc ccagaatgcc gcccatcaca atgaacacca
      661 gaagcaaact tggtaaggca tccagaaacg ctttacaggc ctgagcaaag gttgctcttt
      721 ctgaaagcgg gtagccacgg cgtttagcaa taatgccgca gactatcatt accgcaagac
      781 ccattaagat ccctggcaaa tatcccgcca taaatagcga tgctacagaa acgccgccag
      841 ctgtcaggga aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag
      901 atgagacgtt tacagcggta gaaaatgccg gatcataacc ttgtttggtc tgaatgggat
      961 tcagtgtgcc accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca
     1021 tcatattcgc caggacgttc acatgaccaa gtgaaccagg aacccgacca accattacct
     1081 gggcgaggtt aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt ccggcaaaga
     1141 tgaaaaaggg gattgccaat agggcaaagt tatccagtcc attagccagt cgctgcgaaa
     1201 cagtgattgc cgcgatatcc cagggaaaca tcaaaagcat tgaagctaca gtggcaatac
     1261 cgattgagaa cgaaatgggc acaccaataa agaccaacac aaaaaaacag ccaaacagtg
     1321 taatcgcgat ataactatcc attaactggc ctcctgcgca tgttgtttca gttgttgaat
     1381 gctttgatta ataaaacaca aggcataaaa catcatcacc aatccactta atggcagaat
     1441 gatatatacg tatcccatcg gtatttgcat cgcggcagat acctgtgatg ttgccagcgt
     1501 tttatcaatt aatttcaggc caccagtcac aatgaccgaa ccggcaaaac ctaaaataag
     1561 aacgttaatg actatactaa gaagtaattg cttgcgctta ttcagggcga gagcaaataa
     1621 atcgatagag agatgtctct gcgcgccgac ggtatatgca gcacctaata accctaccca
     1681 gatcatcaaa aatcgtgcca gctcatcggt taatgtactt ggctggttaa gtacgtagcg
     1741 gctaaatact tgccagacta cgcatacaac cagagcaagc atgacggcga cagaaaacgc
     1801 ggcaatgacg cggtctaccg caagtttcag gcggttaaat atcattgtaa gcatcctgga
     1861 aaatcagggc cagagactgg cccggaatat tattgagcag cgttgtcgaa cttctctaac
     1921 aaagcagctt gttttggatc ttttttgaag tcgtcaaaca gaggctgaac tgcggcacgg
     1981 aatggtgctt tatccacttt tacaatttca ccccccatag ctttcgcctg agcacgtgtg
     2041 tcagcgtcaa ttttttccca caatttttgt tgataagctt cggatttctt cgctgctgtt
     2101 tcgaggattt gttgctgttc tggggttaat ttattccatg ttttggtgga gataaccaaa
     2161 aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt tggcaatttc aatatgtcga
     2221 gtctgcaccc aggaaggcac gttattttct gcgccatcta ctacaccttg ctgcatcgca
     2281 gtataaactt cgccaaatga aattggagtg ggtgaaccac ccatcagttc aatcattttg
     2341 atggtggtcg gacttggctg aacacgaatt ttcagccctt taagatcttc tggcttagta
     2401 atcggttttt tcgcatagaa actgcgtgta cccgctacat aagctgataa agcaaaaaag
     2461 cctttgtctt ttgttgagtc cataatttct ttaccaactt caccaaaaac aactttgttg
     2521 aagtgagcct gatctttaaa taagaacggc aaattataga tagcataaat attatcaaaa
     2581 gactccagat cactggcgga acctttggtc atatccagag cgccattttg caacaactcc
     2641 atagtttctc gcgcatcacc catctggctg ctgggataaa tacggatcac catattacct
     2701 tttgacagct gttttacctc tttcgccagc tcttcaaaac tctgatgtac aacatgactt
     2761 ctttccaggt tgtgtgctaa ctttaacgtc actttttctg cggcgtttac gccagtgcta
     2821 accagagcca gcaaggatgc gcagactaat gagcgggtaa atgacctcat tttcataaaa
     2881 aaactccctg ttgaaggatg gaaattggtt gatatctaat tgtgcttatc agtgctttgt
     2941 gcgacaaatt ggttaacctg tctgacaact tgtctatgtt tatcacaact tgaaataaag
     3001 tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
//
LOCUS       DAJSKJ010000264         2906 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000264 DAJSKJ010000000
VERSION     DAJSKJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.268"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(666..1328)
                     /locus_tag="ON345_003794"
     CDS             complement(666..1328)
                     /locus_tag="ON345_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3073771.1"
                     /translation="MTQTVPKGFVRAYLRASTTEQDATRALDTINTFAIERGLSICNY
                     YIENESGSKLERPELFRLLKDCQQNDILLVEDVDRLSRLVGEDWNTLKKMIRQKDIRV
                     VAVNVPTTWLASGHNDFDSRMFSAINDMLLDMLAAVARRDYEQRRERQQQGIARAKRE
                     GKYKGRQVNQSRYDAINRLIASGSSWSQIQKVLGCSRATISKAVKQQTVNKMNHEPEG
                     RE"
     gene            1709..2371
                     /locus_tag="ON345_003795"
     CDS             1709..2371
                     /locus_tag="ON345_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HCR3073772.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     IL"
     gene            2471..2755
                     /locus_tag="ON345_003796"
     CDS             2471..2755
                     /locus_tag="ON345_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR3073773.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVAGKPKLMNFR
                     LPAYFEEILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
BASE COUNT          833 a          708 c          651 g          714 t
ORIGIN      
        1 caatgtgcgt aatgaccccc ctgacctcca acccgtcctc atcgtcatca ggtatagccc
       61 tgaagttatc cagcttatcg agttcctgga gacaccgctt ggggtgtagt cgcaatcgca
      121 tgatcctgaa ttcttgttcc aggacacaga gcaccaaaga cccgtcaacg ggcgtgcccc
      181 caaaatctac tatgagcagc gcaccaggct ttatcccttc ccggaaagag taggtatcac
      241 ttcgaaaaaa gaatactccc ggcgctcctg tattacagtg ctgatcgagt gacagcctgt
      301 tttcctggta gtcggttgct ggcgatggaa aacccatttt taacggcctc cgttggggtt
      361 aaaaagcatg aatgtccggt tctctccttc ctgagtggaa atgtctttaa aaacggtcac
      421 gtaggtttca atccactgat ttgcttggcg acaactccag tgaaaattaa tcagcgcaag
      481 ctggctgaca aaggcttctg tcgtaactgt ctgacgaccg ttcggctcac gcttaatgct
      541 cgcacgccag gctgagtcaa tttcatagtg tctaggcatg aaaaacctcc gatcaatata
      601 gctgtatgca tatacagtat aaagaatcag ttttattctt caacacactg tttattatgt
      661 tgtaactact ccctaccttc aggttcatgg ttcattttgt taacggtctg ttgcttcaca
      721 gctttgctga tagtcgcccg gctacaaccc agcacttttt gaatctggct ccaggagctg
      781 ccgctcgcaa tcagcctgtt tatggcgtca tagcgagact gattaacctg gcggccttta
      841 tacttccctt cccttttcgc cctggcgatc ccctgctgct gccgttcccg gcgctgttca
      901 tagtctcgcc tggcgacggc ggccagcata tccagcaaca tatcgtttat ggccgaaaac
      961 attcggctgt caaaatcatt gtgacccgat gcgagccagg ttgtcggaac gttgacggcc
     1021 acaacccgga tatctttctg ccggatcatt ttcttcagtg tgttccagtc ctcccctacc
     1081 aggcgtgaaa gcctgtccac atcctctacc agcaagatat cgttttgctg acaatctttc
     1141 aggagccgga acaactcagg gcgttcaagc ttagaacctg attcattttc aatgtagtag
     1201 ttacagatgc tcaggccgcg ctcgatggcg aaggtattta tcgtgtccag ggcgcgtgtt
     1261 gcatcctgtt cagtcgtgga tgcgcgtaag taagctctga caaagccttt cggtactgtt
     1321 tgagtcatta tagggaacca gttcattttg agttaaccag acacacccat acagtaccca
     1381 tttcagttgg ttcagggaag gcacactcaa aaagaaccag caaacagacc ccagccctat
     1441 agtgacaacg tcacactaaa aatttacaac ataaagatga tgtaaagaca caccaagatt
     1501 tacaccataa agcgcacata aagacacagc aacatttacc atgtaaagac accataaaga
     1561 taaatatatc tttatttgtg ctttacatgg taaagccatt gcattataaa gacactgtaa
     1621 agacaaaagt atctttacga aaaacacacc atgtaaagac attcatatct ttacatgagc
     1681 tttacaacat aaagagaggg attctgaaat gggtaaaatc ttgcttgtcg tatcagataa
     1741 aggtggggta ggcaaaagca cctacgtggc taacacaggc tcaatgctgg tcaacaaagg
     1801 taagtcggta attatcctga agacagataa aaaccacgat ctgttgagct ggaacgaaaa
     1861 gcggacagat aacggcttac tcactatccc ggttcacgca gcttacggaa acgtcagcaa
     1921 cgaaattaag cgccttagta agctgtgtga agttctcatt gtcgattgcc ccggccacga
     1981 cagccaggag ttccgtagcg ccctgacagt atcagatatc gtggtgacac tggttaagcc
     2041 gtcctctgat ttcgaaagcg aaacactgac cagtgttaca gagaaaatcc gcaccgctca
     2101 gaaagctaac gcagctctgg aaccgtgggt gctgttcacc agaatcaact cagcgaaacc
     2161 ccgccacaga aaagcggcca ttgatctgga taaattactg cgttcggaca acatctggat
     2221 tcaacctctg aaaacccgga tatccgagct ggatgtgtat gaaagcgcct gtaatgaagg
     2281 ggcaggggta catgacgtta gccgggcatc gagcctttca acagcgaaag cacagattga
     2341 acttgtggca caggaaattg gcattttata gaaatatcaa caatattcat cgaaatcttt
     2401 acattgtaaa tacaccataa agatactatg atatttacaa ttaatgcata ccgtacaagg
     2461 ggaaattttc atggctttaa aactaaaccg cccgaacatc gatgaatctc agcaaccagc
     2521 aacaaacgca gaaaccgcgc gttttatctc cggcgctacc agagcgcctg tagcgggtaa
     2581 accaaagttg atgaacttca gactgccagc gtactttgag gagatcctgg aagcggaggc
     2641 gcagagaaca ggccagagca agacaaccgt tctaaaggca gcgctggccg cattcgataa
     2701 cctggacgag aacctgaaaa accactggct gcttgaatct gcaaaattag ggtagcgtta
     2761 tggctaagaa aaagagatat gttgtgatgg ttagagataa accaaataca gcggcaatca
     2821 gcgattatta gcctggttag tatggttcgc taacaggcat aataaggccg ttgcctatga
     2881 ctgcggagaa tggattgtcg aaccat
//
LOCUS       DAJSKJ010000265         2828 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000265 DAJSKJ010000000
VERSION     DAJSKJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.269"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ON345_003797"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ON345_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR3073774.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /locus_tag="ON345_003798"
     CDS             complement(738..1277)
                     /locus_tag="ON345_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR3073775.1"
                     /translation="MNKSMLAGIGIGAAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ON345_003799"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ON345_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR3073776.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNITLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          677 a          777 c          692 g          682 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcgg caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 ggattcggag aagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaaat tcgacagcga taccagtgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aggtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgtg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgagtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt tcgtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttatgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcagagtatt tcaggaacag
     2341 attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgacg cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca tgaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcgtcgc gtagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccaact ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttac gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttat
//
LOCUS       DAJSKJ010000266         2808 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000266 DAJSKJ010000000
VERSION     DAJSKJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.270"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            6..1046
                     /locus_tag="ON345_003800"
     CDS             6..1046
                     /locus_tag="ON345_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR3073777.1"
                     /translation="MTLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGFSAL
                     TLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGISGI
                     IIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEASKV
                     TVAVATVVIFGTVAIFLYPAIYPLMAQWFSPETFGIYIGSTVHEVAQVVAAGHAISPD
                     AENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAIFNS
                     FHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLIVGG
                     GAINYVIQSVIA"
     gene            1120..1977
                     /gene="nfo"
                     /locus_tag="ON345_003801"
     CDS             1120..1977
                     /gene="nfo"
                     /locus_tag="ON345_003801"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR3073778.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTLGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            1981..2808
                     /locus_tag="ON345_003802"
                     /pseudo
     CDS             1981..2808
                     /locus_tag="ON345_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT          668 a          700 c          722 g          718 t
ORIGIN      
        1 cgaatatcac cttacagaaa caacatcgta cactgtggca ttttattccg gggttagccc
       61 tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc cattcccgcg gttgcgggtg
      121 ccgggtttag tgccctcacc ctcgcaatct tgttggggat ggttttaggc aacaccatct
      181 atccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa tatttattac
      241 gtctgggtat cattctttat ggcttccgtc tgacgttctc gcaaattgcc gatgtcggta
      301 tcagtgggat catcattgac gtgttgacgc tgtccagtac cttcctgctg gcttgcttcc
      361 tggggcagaa agtgtttggt ctggataagc acaccagctg gttgatcggt gccggtagca
      421 gtatctgtgg tgctgccgcg gtactggcga ctgagccggt agtgaaagcg gaagccagta
      481 aagtaaccgt ggctgttgca accgttgtta tcttcgggac cgtcgcgatt ttcctctacc
      541 cggcgatata tccgctgatg gctcaatggt ttagtccgga aactttcggt atctacattg
      601 gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg tcatgctatc agcccggatg
      661 cggaaaacgc agcagttatt tccaaaatgc tgcgcgtgat gatgctggct ccgttcctca
      721 tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag aaaagcaaaa
      781 tcactattcc gtggtttgcc atcttgttca tcgtcgttgc catctttaac tcgttccacc
      841 tgttaccgca gagcgtggtg aacatgctgg taacgctgga taccttcctg ctggcaatgg
      901 cgatggcggc gctgggtctg accactcacg tcagcgcgct gaaaaaagct ggggcgaaac
      961 ctctgctgat ggcactggta ctgtttgcct ggctgattgt tggtggtggt gcgattaact
     1021 atgtgattca aagcgtcatc gcataaacca ctacatcttg ctcctgttaa cccgctatca
     1081 ttaccgtttt cctccagcgg gtttaacagg agtcctcgca tgaaatacat tggagcgcac
     1141 gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat cgacgcaacc
     1201 gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct cacgacgcaa
     1261 accatcgatg aattcaaagc cgcctgtgaa aaatatcact acacatcggc gcaaattctt
     1321 ccccacgaca gttatctgat taacctcgga catccggtca ctgaagctct ggaaaaatcg
     1381 cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac tgggactttc tttgctcaac
     1441 ttccaccctg gcagccatct gatgcagatt tcagaagagg attgccttgc gcgtattgcc
     1501 gaatccatca acattgcgct ggataaaact caaggtgtaa cagcggtgat tgaaaacacc
     1561 gccggtcagg gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat tatcgacggc
     1621 gtggaagata aatcccgcgt cggcgtctgc attgatacct gccatgcttt cgctgccggg
     1681 tatgatttgc gtactccagc cgaatgcgaa aaaacgttcg ctgattttgc ccgtattgtc
     1741 ggctttaagt atctgcgcgg gatgcacctt aacgatgcga aaagtacctt gggcagccgc
     1801 gttgaccgcc atcatagcct cggtgaaggc aatatcggtc atgatgcgtt ccgctggatc
     1861 atgcaagacg accgtttcga cggcattccg ctgatccttg aaaccatcaa cccggatatc
     1921 tgggcagaag agatcgcctg gctgaaagcg caacaaactg aaaaagcggt ggcctgatcg
     1981 atgaacgaca gggaaaagca gattcttaaa attttacggc gtaacccgct gattcaacag
     2041 aatgaaatcg ccgatattct gcaaatcagc cgctcacgtg ttgccgctca tattatggat
     2101 ttgatgcgca agggtttgat taaaggcaaa ggatatatcc ttaccgagca ggattactgt
     2161 gtggttgtgg gtgcaatcaa tatggatatt cgcggaatgg cggatatccg ttacccgcaa
     2221 gcagcctctc atccaggttc tgttcactgt tccgcaggcg gcgtcggacg taatatcgcg
     2281 cataacctgg ctctgcttgg tcgggatgtg catttaatat cagctattgg caacgatttt
     2341 tacggcgaga cattactgga agaaacgcgc cgcgcgggcg tgaacgtttc aaactgcatt
     2401 cgtctgcatg gacacagcac tgcaacttat ctcgctatcg ctaataagca ggaagaaact
     2461 atcctggcaa tcaatgacac acatattctg caacagctaa cgcctcaact gctcaatact
     2521 tcacgggact tgattcgcca tgctggtgtg gtgctggctg attgtaatct cacacctgaa
     2581 gccctggagt gggtctttac cattgctgat gaaattccga tgtttgtcga taccgtttcc
     2641 gaatttaaag caaacaaagt aaaaaactgg tacagccgga tccacactct gaaaccaaca
     2701 caaaatgaac tggaaatttt gtggggcaac cgattaaaga cgacaacgat cgcatcagag
     2761 ctgtaaattc gctgcatcaa caaggtgtta aacgcatttt tgtttatc
//
LOCUS       DAJSKJ010000267         2763 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000267 DAJSKJ010000000
VERSION     DAJSKJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.271"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..368)
                     /locus_tag="ON345_003803"
     CDS             complement(<1..368)
                     /locus_tag="ON345_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3073779.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAE"
     gene            555..1760
                     /gene="yedE"
                     /locus_tag="ON345_003804"
     CDS             555..1760
                     /gene="yedE"
                     /locus_tag="ON345_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR3073780.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1757..1990
                     /gene="yedF"
                     /locus_tag="ON345_003805"
     CDS             1757..1990
                     /gene="yedF"
                     /locus_tag="ON345_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR3073781.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            2097..>2763
                     /locus_tag="ON345_003806"
     CDS             2097..>2763
                     /locus_tag="ON345_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR3073782.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLNVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
BASE COUNT          593 a          720 c          735 g          715 t
ORIGIN      
        1 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
       61 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac
      121 atcaatgtca acttgcccgg cacggcaatc acccgctgcc actgacggca atccagggta
      181 tcgccctctt tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc
      241 ggccctttgg tctgccagta gcccgccaac cagtccggcg cgggggtttt cacgacattg
      301 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
      361 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt
      421 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
      481 ccccgaaaat aaatttgcat gatgtatgat tcgcgattct tgtttcaaac cttctgagtt
      541 cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg
      601 cacccatccc tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg
      661 gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt
      721 ttggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat
      781 taacccgcat cgacgggatg atgatcctcg gcatgtttgg cggctgcttt gccgcagcgc
      841 tgtgggccaa caatgtcaaa ctgcgcatgc cgcgcagccg tatccgcatt atgcaggcca
      901 ttattggcgg cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg
      961 cgttctttac cggcattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg
     1021 ccattggttc atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga
     1081 aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc
     1141 gttttcgtct cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga
     1201 tgaaccaacc caaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg
     1261 aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc
     1321 atatggcgaa agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg
     1381 tacagttagg cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta attggtggtt
     1441 tactgtttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg
     1501 cggtagaagg ccaggtgcac tactggtggg tcggtctggg caatgtgatc ggctcgacga
     1561 ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca
     1621 acctgctgaa aacctttggc ccgatgggtg gcctgctggt gacatatttg ctgttgttta
     1681 ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga
     1741 ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga
     1801 accctgccct tatccggcag tcgcaactct tgaggcgatg ccgcagttaa aaaaagggga
     1861 aatcctggaa gtggtgagcg actgtccaca gtcgatcaat aatattccac tggatgcgcg
     1921 taatcacggc tatacggtgc tggatattca gcaagacggg ccgaccattc gttatttaat
     1981 tcagaagtaa tccctcattc ccgctgtggc ccacagcggg aacatcgttg cctctgacac
     2041 atagatttta cccgccccct ctcctattct tttcagtatg atctcaggag gtttctatgt
     2101 gtggacgctt tgcccaatcc caaacgcgtg aagattacct tgcgcttctc gcggaagata
     2161 ttgaacgcga tattccctac gatcccgaac ccattggcag atacaacgtc gcgccgggaa
     2221 ccaaagttct tctgctcagt gaacgtgatg aacaccttca tctggatccg gttttctggg
     2281 gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta gaaactgcgg
     2341 ccaccagtcg tatgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg
     2401 gctggtttga gtggaaaaaa gaaggcgaca aaaaacagcc ttattttatc tatcgcgctg
     2461 atggacaacc tgtttttatg gccgcgatag gtagcacacc gtttgagcgc ggtgacgaag
     2521 ccgaaggatt tttaaatgtc actgcagcag cagaccaggg tctggtagat attcatgacc
     2581 gccgcccgct ggttctgtcg ccagaagctg cacaggaatg gatgcggcaa gagattggcg
     2641 gtaaagaagc atcagaaatt gctaccaacg gctgtgtgcc agcaaaccag ttcacctggc
     2701 acccggtatc gcgcgcggta ggtaatgtca aaaaccaggg cgcggagtta attcaacctg
     2761 ttt
//
LOCUS       DAJSKJ010000268         2759 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000268 DAJSKJ010000000
VERSION     DAJSKJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.272"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1115
                     /locus_tag="ON345_003807"
     CDS             <1..1115
                     /locus_tag="ON345_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR3073783.1"
                     /translation="LILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIF
                     RQLSFIYLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSL
                     YVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCN
                     LLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNR
                     RYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRP
                     DDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTV
                     FSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1296..>2759
                     /locus_tag="ON345_003808"
     CDS             1296..>2759
                     /locus_tag="ON345_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR3073784.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQGIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTNCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYNHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKCL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVN"
BASE COUNT          732 a          607 c          599 g          821 t
ORIGIN      
        1 cactcatttt aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa
       61 ttatccataa accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta
      121 ttttctatat tttccgccaa ctcagtttta tttatttaac ctcactggca ctcttttgtt
      181 atggaaaaga caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc
      241 tgattccttt tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact
      301 attctttgta tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact
      361 atacaaaaat attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac
      421 gattagccag cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca
      481 acttacttct actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga
      541 ttgaagtttc cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc
      601 tgcaactctc cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct
      661 actttttcaa cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca
      721 tgctggttga tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg
      781 ataaagtgct ggtttcaatt gtcgatatta tccagcaaag catccgccct gatgatattt
      841 tagcgcgact ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg
      901 caaaaatcat tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca
      961 gatatgatgt tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca
     1021 cgcgtaatat ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg
     1081 aggggggcaa caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct
     1141 ttttgcgtgg cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg
     1201 gcgcagagat gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt
     1261 ttctcgtttc actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc
     1321 ggcattttat cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg
     1381 tcgccagcga cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct
     1441 cctttcttta tgataagtat cagaatcagg gtattgccgc gcatgtgatg cgcgctctcg
     1501 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
     1561 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
     1621 cactacaaac cgcatccact aactgcgaca caattgtgga agctgcagca ctattacccg
     1681 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
     1741 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
     1801 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
     1861 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
     1921 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
     1981 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
     2041 tttacaatca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
     2101 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
     2161 acgtccatga cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
     2221 tcacgtcttc gtggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
     2281 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
     2341 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
     2401 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
     2461 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttatta gctcaggcga
     2521 ttaaacaatc gattcgtaaa agtgattatg ccatccgact cggtggcgat gaattctgca
     2581 tcattcttgt cgattcgacg ccgcaaattg cagcacaact gcctgaacgt atcgaaaaat
     2641 gtctgcaaca tatcgcgccg cagaaagaaa tcggcttctc ttccggtatt tacgcgatga
     2701 aagaaaacga tacgttacat gatgcgtata aagcttccga tgagcgttta tatgtcaat
//
LOCUS       DAJSKJ010000269         2711 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000269 DAJSKJ010000000
VERSION     DAJSKJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.273"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            332..>2711
                     /gene="pgaA"
                     /locus_tag="ON345_003809"
     CDS             332..>2711
                     /gene="pgaA"
                     /locus_tag="ON345_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HCR3073785.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDR"
BASE COUNT          751 a          625 c          634 g          701 t
ORIGIN      
        1 gccgtagcgc agcctttcat caggactttc gaaaaatccg aaatcatgca tcggaattta
       61 ctgatttaat tattttaatc ctaatttatt ttgaaaaagg cattgggatt tatgccgtat
      121 tcctgaagat cctcatcatt ggaatggatt ttcgggcgag aaaaggattt tatatggaca
      181 ctctgctcat catttcttct tctcatcatc aacaattcac gtctctcttc cgcgtttaat
      241 aacggattat gaggtgccaa aatatctttc ttttcagtta cctgtaatta gatacagaga
      301 gagattttgg caatacatgg agtaatacag gatgtattca agtagcagaa aaaggtgccc
      361 gaaaaccaaa tgggctttga aacttcttac tgccgcattt ttagccgcga gtcccgcggc
      421 gaagagtgct gttaataacg cctatgatgc attgattatt gaagctcgca agggtaatac
      481 tcagccagcg ttgtcatggt ttgcactaaa atcagcactc agcaataacc aaattgctga
      541 ctggttacag attgccttat gggccgggca agataaacag gttattaccg tttacaaccg
      601 ctaccgtcat cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg cttatcgtaa
      661 cctgcaacaa tggcaaaact cgcttacact gtggcaaaag gcgctctctc tggagccgca
      721 aaataaggat tatcaacggg gacaaatttt aaccctggca gatgctggtc actatgatac
      781 tgcgctggtt aaacttaagc agcttaactc tggagcaccg gacaaagcca atttactcgc
      841 agaagcctat atctataaac tggcggggcg tcatcaggat gaattacggg cgatgacaga
      901 gtcattacct gaaaatgcat ctacgcaaca atatcccaca gaatacgtgc aggcattacg
      961 taataatcaa cttgctgccg cgattgacga tgccaattta acgccagata ttcgcgctga
     1021 tattcatgcc gaactggtca gactgtcgtt tatgcctacg cgcagtgaaa gtgaacgtta
     1081 tgccattgcc gatcgcgccc tcgcccaata cgctgcatta gaaattctgt ggcacgataa
     1141 cccagaccgc actgcccagt accagcgtat tcaggttgat catcttggcg cgttattaac
     1201 tcgcgatcgt tataaagacg ttatttctca ctatcagcga ttaaaaaaga cggggcaaat
     1261 tattccgccc tgggggcaat attgggttgc atcggcttat ctcaaagatc atcagccgaa
     1321 aaaagcacag tcaataatga ccgagctctt ttatcacaag gagaccattg ccccggattt
     1381 atccgatgaa gaacttgcgg atctctttta cagccacctg gagagtgaaa attatccggg
     1441 cgcgctaact gtcacccaac ataccattaa tacttcgccg cctttccttc ggttaatggg
     1501 cacgcctacg agcatcccga atgatacctg gttacagggg cattcgtttc tctcaaccgt
     1561 agcaaaatat agtaatgatc ttcctcaggc tgaaatgaca gccagagagc ttgcttataa
     1621 cgcaccagga aatcagggac tgcgcattga ttacgcgagt gtgttacaag cccgcggttg
     1681 gcctcgtgca gcagaaaatg aattaaaaaa agcagaagtg atcgagccac gtaatattaa
     1741 tctggaggtt gaacaagcct ggacagcatt aacgttacaa gaatggcagc aggcagctgt
     1801 cttaacgcac gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac gattaaaacg
     1861 tgcggttgat gtacataatc ttgcagagct tcgtatcgct ggctcaacag gaattgatgc
     1921 cgaaggcccg gatagtggta aacatgatgt cgacttaacc accatcgttt attcaccacc
     1981 gctgaaggat aactggcgcg gttttgctgg attcggttat gccgatggac aatttagcga
     2041 aggaaaaggg attgttcgcg actggcttgc gggtgttgag tggcggtcac gtaatatctg
     2101 gctcgaggca gagtacgctg aacgcgtttt caatcatgag cataaacccg gcgcgcgcct
     2161 gtctggctgg tatgatttta atgataactg gcgtattggt tcgcaactgg aacgcctctc
     2221 tcaccgcgtt ccattacggg caatgaaaaa tggtgttaca ggcaacagtg ctcaggctta
     2281 tgttcgctgg tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt tcactgattt
     2341 ttccgacagt aaccagcgtc atgaagtctc acttgagggt caggaacgca tctggtcttc
     2401 accatatttg attgtcgatt tcctacccag tctgtattac gaacaaaata cagaacacga
     2461 taccccatac tacaacccta taaaaacgtt cgatattgtt ccggcatttg aggcaagcca
     2521 tttgttatgg cgaagctatg aaaatagctg ggagcaaata ttcagcgcag gtgttggtgc
     2581 ctcctggcaa aaacattatg gcacggatgt cgtcacccaa ctcggctacg ggcaacgcat
     2641 tagttggaat gacgtgattg atgctggcgc aacgctacgc tgggaaaaac gaccttatga
     2701 cggtgacaga g
//
LOCUS       DAJSKJ010000270         2680 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000270 DAJSKJ010000000
VERSION     DAJSKJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.274"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(89..1627)
                     /gene="cadC"
                     /locus_tag="ON345_003810"
     CDS             complement(89..1627)
                     /gene="cadC"
                     /locus_tag="ON345_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HCR3073786.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          789 a          538 c          558 g          795 t
ORIGIN      
        1 aaataaattg ccgcaacata ttataccaac gggaacatac aaaaactcaa caacaaatat
       61 tccagagcat aaatcaaccc ggagttactt attctgaagc gagaaatttg tcgagataag
      121 gtacaacata aggaacagaa gtctggaata taccattttc aatccagtaa agggtgtttg
      181 cccctgggcg taaattaaag gcggtgagat atgcatcagc tgcttcccgg ttcatcccct
      241 tcatttcata aaccttgcca agcaacacat aatttagcca ggacatttca agatcaatgc
      301 cagtatttat cgcctggtaa gactcatctg ttttaccttt taccagagca ctgaccgctt
      361 ttatttgata tataatggat aggttattca attccggtag tgtaacaatg ttgtctattt
      421 ctgtgttcag tgctgctaat tgtttttcat ctaaagggtg ttgagaatgg cgcacgatat
      481 caactaatgc tttttctgct ctcgcgtagg taaattctgg ggatgattga acaatctcac
      541 ctaataattc actggcacgg ttcaatgatt tatcatcgcc atgcagtaaa taatcatgtg
      601 cctgataaaa attagttaat aacgcaccac gatgaggcaa aatttgctgg agcgtctcct
      661 gcattcgttg tggccacggt tggtttaatg cttttgataa actctccagt aaatcatttt
      721 gaatcgccag ctgattaccg ttagtgatga cataacgttt atccagcatg gttgaaccat
      781 ctgcattgtc taccaatttt atcgacataa agcattgttg agcacggtat tggcgctgat
      841 taacaaaggc aatagataat gttttaccgg aactgctcgg ttcatcaatg ttgtagttga
      901 ttttgtcatg caccataaag gtggagaagg tgttaagtga tgtcgccacc aaatcaccca
      961 cgcctatcgc gtaagagagc tgatacgggg aactccagct gttacaactt ttatttacca
     1021 tattaatgtc aatatcgcgt ggattgagca aaatacgcga tttgctcata ggaagacgtg
     1081 tatcaagagt tgaaaacgct accagtgcta cacagatacc taacgacaac aggaaaaaaa
     1141 accataccca aaaggtagtg aatcgtttgc ttttaactgg ggattgttca ggtggcgttg
     1201 cggtgttttg aatgttaaga ctgtgggagg gagaatctgt ggcaggaacc gcctctggta
     1261 tagggggagg cgaagatagc attatttcct ctccctcttc ttcgctgtac cagataaccg
     1321 gcaccattaa tttatagccg cgctttggta cagtagcgat atagacagga ctatcttcat
     1381 cattatcttt taatgactta cgtagttctg agatactctg cgtcacaaca tgattggtga
     1441 caatacttct cttccagaca ttatcgataa gttcatccct gctaagtact tcgccactgt
     1501 gttgagcaaa gaaaaccaga agatcgatta atctcggctc aagggtaagt tgacgcccat
     1561 tgcggctaat ttggtttatg gacggagtaa caagccattc gccaacgcga actacaggtt
     1621 gttgcataat agaaactcat tcgaaaaggg aatgatgcaa tgataattgc cacaacctat
     1681 ttttaccatc tatagatggg tttatttaca tattattggt gaatgtaaga cgttattttt
     1741 accagccata aacttcttga ttacatagta ttacgaaagg attttactga gaaccagaag
     1801 taatattcct taccatcaaa attcatcatc tttgccaaag aaaaatgttc agaaaataat
     1861 ccatggaaaa ttgtccggag cgcttactat tttaatggat tgttagtctt tgcatgagca
     1921 agcggactga tacatttctc tttgttctca ttcagaaaat ctcatcagtc ggcgttcaga
     1981 ccggtgttgt gctttatgga tgcgttacgc tcctgatgac gtcatttgac gttcaacaac
     2041 atcacggggc cgcacgacat ttcacgtcag ttagtgctat cgctcaggaa caaattttcc
     2101 cgaattggga tatgcccgca aattgctggt gatgtgggga gaatctggtt gagttcggga
     2161 gaattgattt ggaggcagaa cgcttaaatc gtggcgtcct gaaacgaaaa acggacctcc
     2221 gtggaggtcc gtttatatga atttggtgcc cggactcgga atcgaaccaa ggacacgggg
     2281 attttcaatc cctttaaaag agcccaaacg atccatcagc actaacagtt ttttactggt
     2341 ttaccttgag aaaaatgttt aagtttggtt cttcatgact taagtattgc ttaaatacgg
     2401 cgtttcgaag ctaccagaat cctaccatta tgagcctata cccatagaag agaaattatc
     2461 tcccgttctc gcgattcaat gactaaaaat cagacggtgc aggcttttag atactgatat
     2521 tatatctttt gggtaaagta ttaatctctg ttgagatagg ctcttagccc taaaatagcc
     2581 gagcagcaaa cgccaaaaca acaatactga cattaccatt aactgtcgcg atggttctga
     2641 gtcgatcgtc tttaagtgtt ttaccatcaa atgttgcgat
//
LOCUS       DAJSKJ010000271         2647 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000271 DAJSKJ010000000
VERSION     DAJSKJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.275"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..153)
                     /locus_tag="ON345_003811"
     CDS             complement(<1..153)
                     /locus_tag="ON345_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCR3073787.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYT"
     gene            397..654
                     /locus_tag="ON345_003812"
     CDS             397..654
                     /locus_tag="ON345_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR3073788.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(704..1654)
                     /gene="uspE"
                     /locus_tag="ON345_003813"
     CDS             complement(704..1654)
                     /gene="uspE"
                     /locus_tag="ON345_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR3073789.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1806..2558)
                     /gene="fnr"
                     /locus_tag="ON345_003814"
     CDS             complement(1806..2558)
                     /gene="fnr"
                     /locus_tag="ON345_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR3073790.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          684 a          615 c          632 g          716 t
ORIGIN      
        1 ggtgtagatt aaaaattgca ccgcatggaa taaaaaacct tttcttttac gatttccgcg
       61 gcgaaaccaa aagctcatca gaatcaatgc tgcgcagcta ccgaaaataa tgaccagatt
      121 aagcgcatta tttgtaaaca gttcagcgat cattgtttta tcaggctcct ccagataatt
      181 gtcgtcatgc cggaaacccc tggcggggct attttaccgc gacaattcat tcagatcatc
      241 aatagtcagg gaaggaagta gcaacattag ctaactaatc aatgcgttga ttgtaaatcc
      301 agctaagagg tgaggttttc agagcagaca acggtgaaat gtcatggtat tgttacgttt
      361 aggtaacaag aaatttgtct gcacaaggat tacatcatga ttatggcgaa actgaagtca
      421 gcgaaaggga agaaatttct ctttggtttg ttggcggttt tcattattgc ggcgtcggtt
      481 gtgactcgcg cgactatcgg cggcgttata gaacagtaca atattccgct gtctgagtgg
      541 acgacatcaa tgtatgtgat tcagtcatcg atgatttttg tttatagcct ggtctttact
      601 gtgttgctgg caatcccgtt gggaatttat ttccttggcg gcgaagagca gtaagtaaaa
      661 aataggcccg ataactcggg ccttgtcagt tattgaagag tcgttaatcg tcttcttcgt
      721 catccagttc aacgggtgtc tgatactggt caggtttaat gaccaacaga tcgcagcgaa
      781 gatgatcaat cacctgttcc gccgtgttgc cgaggaatgc tgctgaaata ccggtgcgtc
      841 ctaccgtgcc cagaaccaca atccccgcct gtaagtgttc cgccaaatca ggaatcacct
      901 cttctggcag acctttttct acgtgcgtca tgttttcatt aatgccgaat ttctgccgca
      961 gagctttcat tgccagcaaa tgttgcccac gaatggcatc gttataaacg ctcggatcaa
     1021 attccggcag ttcaatcgcg atattaattg gcgttaccgg ataagcgcca accagatgaa
     1081 cttcggtatg gttgacttgt tctgccagtt cgatcgtctc tttgaccagt ttttcattga
     1141 gcgcattatg atacggctct tcactggcga gattcaccgc caccagcgcc ttgcctcctt
     1201 ccggccacgg ctggtctttc accatccaca ccgggcttgg gcatttgcgt aacagatgcc
     1261 agtccgttgg cgtaaaaatc accgcttcca gacggtcatg ttggtgcgcc atttttagca
     1321 ccaaatcgtg cccgccgctg atcacttcct gaatgatggc ttcgaaagga cggttatgcc
     1381 agaccacttt aatttcaatg ggaacgccag cattgagata atattttgcc tgctcgtgga
     1441 tccaggctgt acgctggctg atgacgccct gacgcatagc ggtacgttcg tccggggaga
     1501 gcagggtggt catttcgtat gagaagtcat agatcggcaa aaaggcttta attttgccac
     1561 caatccgttg atgtaaataa acagctcgcc gcaatgctgg ttggtcgtcc tggttaggat
     1621 cgataacaac gagcatgttc tgatacatag ccatacaggg tctccttaca acaactgtca
     1681 acgcagtttg taattaaaag attaacccat atctggtgaa tgaaacagtg atgaaccttc
     1741 tgccagatca ataaatcaga aaaatttaat gatatgacag aaggatagtg agttatgcgg
     1801 aaaaatcagg caacattacg cgtatgacca gcaagctggg ccagcgcatc gttattttcg
     1861 atggtgatgt atttaccttt gactgccagc ataccgcttt tctggaagcg acctagcaga
     1921 cggctgatgg tttctaccgt caggcccaga tagttaccga tatcgccacg agtcatcgtc
     1981 aggcggaatt cacgagggga gaagccgcgt tgggcaaaac gacgggacag gttgtagatg
     2041 aatgcagcca gacgttcctc ggcatttttc ttcgacaaca gcaggatcat gtcctgatcg
     2101 cctttgattt caccgctcat cagacgcatc atctgctgac gcagattcgg cattttaccg
     2161 gacaaatcgt ccagcgtttc gaacgggatt tcacatacca tcgaggtttc cagcgcctga
     2221 gcgaagctcg ggtgatggcc gctgccgatg gcgtcaaatc ccaccaggtc gccagctaaa
     2281 tggaaaccag tgatttgctc gtcgccttgc tcagtgatgg tataactttt aatcgtaccg
     2341 gagcggatgg cataaagcga tttaagttca tcaccagcct taaacagcgt ctggcctttc
     2401 tgaataggct tcttccgctc aatgatatta tcaagctgat caagctcatg ttcgttgagt
     2461 gtgaacggga tgcaaagctg gctgatgctg caatcctggc aatggatagc acaaccgcca
     2521 gactgaatgc gccgtataat tcgcttttcc gggatcatag gtctgctcaa gccgtaattg
     2581 atatttgtca attttaacat ctttttaggg agcaagtaag tctaagcaaa ccttaacagc
     2641 agagaat
//
LOCUS       DAJSKJ010000272         2616 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000272 DAJSKJ010000000
VERSION     DAJSKJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.276"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            455..598
                     /locus_tag="ON345_003815"
     CDS             455..598
                     /locus_tag="ON345_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR3073791.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            611..820
                     /gene="cspE"
                     /locus_tag="ON345_003816"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             611..820
                     /gene="cspE"
                     /locus_tag="ON345_003816"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR3073792.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            986..1795
                     /gene="rlmA"
                     /locus_tag="ON345_003817"
     CDS             986..1795
                     /gene="rlmA"
                     /locus_tag="ON345_003817"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR3073793.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1792..2364)
                     /gene="mntP"
                     /locus_tag="ON345_003818"
     CDS             complement(1792..2364)
                     /gene="mntP"
                     /locus_tag="ON345_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR3073794.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEDEEPR
                     RRHGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFI
                     GSIIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          689 a          618 c          622 g          687 t
ORIGIN      
        1 ttatttatta gcgaaacgtt tctctcttga tttttttgct cattcatcaa tttttcttat
       61 tttaaatttc caataccttt gggattgact tctctttggg gtaattgata gccgttaact
      121 gactgtttta tgagaaaaag tgatataact ttttattcat tgcatagcaa aaaatgtgat
      181 attgcacgca ctatgtaata acttctccca ctggcctgga acaactgaac ttattgaact
      241 atgttagaaa atacgccagt ttaagtatct gcctgaactg gcaaggttaa gcacaatgat
      301 atatcggcgc gtattccgtt gcataagtgt gcaaaaaaag tggaagacgt atcgagattt
      361 gtgcgtctga tcgagacatg tttaaaaatg gcttgccata attaacgttg tatgtgataa
      421 cagatttcgg gttaaacgag gtacagttct gtttatgtgt ggcattttca gtaaagaagt
      481 cctgagtaaa cacgttgacg ttgaataccg cttctctgcc gagccttata ttggtgcctc
      541 atgcagtaat gtgtcagttt tatctatgtt atgcctgcgg gcgaagaaaa caatctaagg
      601 aatttttcaa atggcaaaga ttaaaggtca ggttaagtgg ttcaacgagt ctaaaggttt
      661 tggcttcatt actccggctg atggcagcaa agatgtgttc gtacacttct ccgctatcca
      721 gggtaatggc ttcaaaactc tggctgaagg tcagaacgtt gagttcgaaa ttcaggacgg
      781 ccagaaaggt ccggcagctg ttaacgtaac agctatctga tcgaatccac tgatctgaag
      841 tgtgaatacg cttcaatctc gctataaagc ctcgtcgaat gcgaggcttt ttactatgct
      901 ttatcttcgc tcctggcgtt cggatatttg cccgccgcgt gattcgcgtt acacttgcgg
      961 cctttagtat cctgccggag ttgtcatgtc gttttcctgt ccactttgcc atcagcctct
     1021 ttcgcgtgaa aaaaacagct atatctgtcc ccagcgacat cagtttgata tggcgaaaga
     1081 agggtatgtc aatctgctgc ccgttcagca taaacggtct cgtgatccgg gcgacagcgc
     1141 ggaaatgatg caagcacgcc gcgcattctt agatgccgga cattatcagc cgctgcgtga
     1201 tgcaattgtc gcccaactgc gggaacggct tgatgaaaag gccacggcgg tgctggatat
     1261 tggctgtggt gaagggtatt acacacacgc atttgccgat gcgttgcccg aaatcaccac
     1321 gtttggcctg gatgtctcga aggtagcgat aaaagcggcg gcgaaacgct atccgcaggt
     1381 cactctttgt gtcgcttcca gccaccgttt gccgttttcc gataccagta tggacgccat
     1441 aatacgtatt tacgcgccgt gtaaagcaga agaattagca cgagtagtga agcccggcgg
     1501 ctgggtcatt actgccacgc cgggaccgcg acatttgatg gagctgaagg ggctgattta
     1561 caatgaagta catcttcatg cacctcatgc agaacaactg gaaggtttta cattacagca
     1621 gagtgatgag ttgtgttatc cgatgcgtct tcgcggtgat gaagccgtcg cattattgca
     1681 gatgacgccg tttgcctggc gtgcgaagcc agaagtctgg caaacactgg cagcaaaaga
     1741 agtgttcgac tgccagacgg actttaatat tcacctctgg cagcgttctt attaaccgtg
     1801 gaagtgcgtc cagaggatct ggacgccgat gccgatcagc accagcccgc cgagaatttc
     1861 cgcttttttc ccaataattg agccgataaa gcgaccaacc atcatcccta atgtcgacat
     1921 aatcaaggtt gcacaaccaa tggccaatgc ggtcgcgata atgttgacct gcaggaaagc
     1981 aagaccaaca cccacagcca tggcatccag gctggtggca atcgcggtgg ttaccagtag
     2041 ccagaaaccg tgccgacggc gcggctcttc atcttcatct tcatcatctg cgccacgaaa
     2101 accctcaata atcattcgcc cgccaaggaa tatcagcagc acaaacgcaa tccagtgatt
     2161 ccattcaagg acaaaccggc tggctaacat gcccattccc cagccgatca gcggcgtcag
     2221 ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt tcggtttatg
     2281 gagggtggca cctttaccga ttgatgcagc aaatgcatcc atcgacatac caaacgcaag
     2341 aagaacagta gcagtgatat tcatgacaac gtcctgaccg gggtatccat aaaacacatc
     2401 atcgccccca gcaaacatgc atacatgaac aacaagccgc gatgggtttt cgcgttcatg
     2461 caggcatagc gattgatgtg catatggtct cgcctgactg aaagtattca gtccgtacgt
     2521 gccacgtttt gcaacgagta tgttgacacg tacatttccg gaatctggaa atcggctact
     2581 ccccaaatga gggcgcaacc ttaacatatt ttgaga
//
LOCUS       DAJSKJ010000273         2594 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000273 DAJSKJ010000000
VERSION     DAJSKJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.277"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            117..1730
                     /gene="mppA"
                     /locus_tag="ON345_003819"
     CDS             117..1730
                     /gene="mppA"
                     /locus_tag="ON345_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR3073795.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1781..>2594)
                     /gene="ynaI"
                     /locus_tag="ON345_003820"
     CDS             complement(1781..>2594)
                     /gene="ynaI"
                     /locus_tag="ON345_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR3073796.1"
                     /translation="TPGVIDFICTSMIAVILTIKLFLLINQFEKQQIKKGRDITSARI
                     MSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYF
                     DRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMT
                     NRRITTTIGLRYEDAEKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVY
                     CFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          742 a          591 c          626 g          635 t
ORIGIN      
        1 gatacataaa acattttatg cttcctgtgt cacttttttg atggaagttg tttgcatttc
       61 tttaaggcga aacaaataat tacgcatcaa ttttaatgtc ggttagaggg aaacttatga
      121 agcactctgt ttcagtcacg tgttgtgcgc tgttggtcag cagcatttct ctttcgtatg
      181 ctgcagaagt tccgagcggc acagtactgg cagagaagca ggagctggtg cgccacatta
      241 aagatgagcc tgcgtcgctg gatcccgcta aagccgtggg cctgccagag attcaggtca
      301 ttcgcgatct gtttgaagga ctggtgaatc agaacgagaa aggggagatt gtccccggcg
      361 ttgcgactca gtggaaaagt aatgacaacc gtatctggac ttttaccctg cgcgataacg
      421 caaaatgggc ggatggcaca ccggtaacgg cgcaagattt tgtctacagc tggcaacgtc
      481 tggtggaccc gaaaacgcta tcgccatttg catggtttgc cgcgctggcg ggaatcaaca
      541 acgcacaggc gattattgat ggtaaagcta cgccagacca gcttggcgtc accgcagttg
      601 atgcccatac tttgaaaatt cagcttgata aaccgttgcc gtggtttgtg aatttaaccg
      661 ctaactttgc cttcttcccg gtgcaaaaag ccaacgtaga aagcggtaaa gagtggacga
      721 aacccggaaa tctgatcggc aatggcgctt atgttcttaa agatcgcgta gtcaatgaaa
      781 aactggtcgt ggtaccgaat acccattatt gggataacgc caaaacggta ctgcaaaaag
      841 tgaccttcct gccaattaat caggaatccg cagccactaa gcgttacctc gcgggggata
      901 ttgatatcac cgaatccttc ccgaaaaata tgtatcagaa gctgttgaag gatattccgg
      961 ggcaggttta tacgccgcca cagctcggga cctattatta tgcgtttaac acgcaaaaag
     1021 ggccgacggc agatcagcgc gttcgcctgg cattaagtat gacgatagat cgccgcctga
     1081 tgaccgaaaa agtgttggga acgggcgaaa agccagcgtg gcattttaca ccagatgtta
     1141 ccgccggatt tacgccggaa ccttcgccgt ttgaacaaat gagtcaggaa gaactgaatg
     1201 cgcaggcaaa aactttgttg agcgcagctg gttatggtcc gcaaaaaccg ctgaagctga
     1261 cgcttttgta taacacttca gaaaaccatc aaaaaattgc gattgctgta gcatcgatgt
     1321 ggaaaaagaa ccttggcgta gatgttaaat tgcaaaatca ggaatggaaa acctatatcg
     1381 atagccgtaa caccggcaat tttgatgtta tccgcgcctc atgggtgggg gattataatg
     1441 aaccctccac tttcctgaca ttattaacgt caacgcattc aggaaatatt tcacgcttta
     1501 acaatccggc atacgacaaa gttctggccc aggcatcgac ggaaaatacc gttaaagcgc
     1561 gtaatgccga ttacaacgcg gcagaaaaaa tcctcatgga gcaagcaccg attgcaccaa
     1621 tttatcaata taccaatgga cgattaatca agccgtggct gaaaggttat cccattaata
     1681 acccggaaga tgtggcgtac agccggacta tgtatattgt gaagcattga tgtgatggga
     1741 actggcgtta cccttgtgga taacgccagt gatgtctgat ttagcgcccc tgttccggcg
     1801 gtgtaatgtt atccatatac agcgtctggc tggggaaggc aaagtccgcg ccgtgtgact
     1861 gcacaatatc gataatcttc aaataaacgt cttgctgtgc agcaagccat tcagcccata
     1921 ccgtggtttt ggtaaagcaa taaaccataa tattcaatga cgagtcggca aactggttga
     1981 aataaaccag taaggtttgt cgctggtcga tggccgggtg atttttcagc atctcacgta
     2041 cggcttcgac aataacgccc actttttccg catcctcata acgtaaacca atggtcgtgg
     2101 taatgcggcg gttggtcatt cgtcctgggt tttctacgct gatcgacgaa aacagcgagt
     2161 tcggtacgta caatggacga ttatcaaagg tcgtaatttt ggtaattcgc cagccaattt
     2221 ccgctactgt accttcgata tttctgtccg gtgaacggat ccagtcgcca atactgaaag
     2281 gacggtcgaa atagagcatg atcccggaaa agaaattact cagaatatct ttaccggcca
     2341 taccgacagc cagaccacca ataccaccaa aggtcagcaa gccagaaagg ctcatgccga
     2401 aatgttcgcc ataaagcaga acaagcacca caataatggt gattttgata atgcgcgaca
     2461 taatccgcgc actggtgata tcgcgacctt ttttaatctg ctgtttttca aactgattaa
     2521 tcagcaaaaa tagcttaatc gtcagaataa ccgcaatcat ggacgtacag ataaaatcga
     2581 taacgccagg ggtg
//
LOCUS       DAJSKJ010000274         2593 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000274 DAJSKJ010000000
VERSION     DAJSKJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.278"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..474
                     /gene="ydiB"
                     /locus_tag="ON345_003821"
     CDS             <1..474
                     /gene="ydiB"
                     /locus_tag="ON345_003821"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR3073797.1"
                     /translation="AGGASTAIGAQGAIEGLKEIKLFNRRDEFFDKALAFAQRVNENT
                     DCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVNDISLLHPGLLVTECV
                     YNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFPLEYVKQVMGFGA"
     gene            505..1263
                     /gene="aroD"
                     /locus_tag="ON345_003822"
     CDS             505..1263
                     /gene="aroD"
                     /locus_tag="ON345_003822"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR3073798.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            1407..>2593
                     /locus_tag="ON345_003823"
     CDS             1407..>2593
                     /locus_tag="ON345_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HCR3073799.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGK"
BASE COUNT          680 a          600 c          667 g          646 t
ORIGIN      
        1 gccggaggtg cctcaacggc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
       61 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
      121 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
      181 gaagccctgg cttccgccga cattttaacc aatggcacaa aagtgggtat gaaacccctt
      241 gagaatgaat cattagttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
      301 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cgcaacaagc tggttgcaaa
      361 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
      421 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
      481 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
      541 acgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
      601 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
      661 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
      721 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
      781 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
      841 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
      901 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
      961 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     1021 ctgatgccgc aaagtaccag cgatgtgctg acgttgcttg ccgcgaccct ggagatgcag
     1081 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     1141 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     1201 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     1261 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     1321 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     1381 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     1441 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     1501 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     1561 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     1621 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     1681 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     1741 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     1801 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     1861 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     1921 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcgcgct accacctgcg
     1981 acagtgaagg ctacgccact tttgaagatg aggtgatgta tctcgacgca ttggttattg
     2041 cccaggcggt gcacaataac ggcggtattg tgatgatgca ggtgcagaaa atggttaaga
     2101 aagccacgct gcatcctaaa tctgtccgta ttccgggtta tctggtggat attgtggtgg
     2161 tcgatccgga tcaaagccaa ctgtatggcg gtgcgccggt taaccgcttt atttctggtg
     2221 acttcaccct tgatgacagc accaaactta gcctgcccct aaaccaacgt aaattagttg
     2281 cgaggcgtgc attattcgaa atgcgtaaag gcgcggtggg gaatgtcggc gtcggtattg
     2341 ctgacggtat tggcctggtc gcccgggaag aaggttgtgc tgatgacttt attctgacgg
     2401 tagaaacagg tccgattggc ggaattactt cacaggggat cgcctttggc gcgaacgtga
     2461 atacccgtgc cattctggat atgacgtccc agtttgattt ttatcacggt ggtggtctgg
     2521 atgtttgtta tttgagtttt gctgaagtcg accagcacgg taacgtcggc gtgcataaat
     2581 tcaatggcaa aat
//
LOCUS       DAJSKJ010000275         2542 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000275 DAJSKJ010000000
VERSION     DAJSKJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.279"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            76..1008
                     /locus_tag="ON345_003824"
     CDS             76..1008
                     /locus_tag="ON345_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR3073800.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1049..2479)
                     /locus_tag="ON345_003825"
     CDS             complement(1049..2479)
                     /locus_tag="ON345_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR3073801.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          664 a          565 c          526 g          787 t
ORIGIN      
        1 ctccttaagg atcatccgtt atttgggtcg ttttctgcta aaatctgcgc cttgcgcttc
       61 tttagcgcac tgtctatggc taaccttttg aataaattca ttatgacgag aatactcgct
      121 gcgataaccc ttttgctgag tatcgtattg accattttgg tcactatttt ttgttccgtc
      181 ccgatcatca ttgccgggat tgtaaaacta ttgctacctg tgccagtcat ttggcgaaag
      241 gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg aaggtctggc ggtattactg
      301 catctgaacc cacacctgca atgggaagtt cacgggctgg aagggttaag taagaagaac
      361 tggtatctgc ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact gtgcgtgctg
      421 tttcgtaagc acattccgat gaataaatat ttcctcaagc aacagctggc ctgggtgcca
      481 tttcttggcc tggcgtgctg ggcgctggat atgccgttta tgaagcgtta ttcccgcgct
      541 tatttattac gtcatccgga gcgacgtggt aaagatgttg aaaccactcg gcgttcttgt
      601 gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg ttgaaggctc ccgtttcacg
      661 caagaaaaac atcagcaaac ccactccact tttcaaaact tgttgccacc aaaggcggca
      721 ggcattgcga tggcgctaaa tgtactgggt aaacaattcg ataaactgct taatgttacg
      781 ctgtgttatc cggacaataa ccgtcagccg ttcttcgata tgttaagcgg taaactgacg
      841 cgaattgtcg ttcatgtgga tttacagcct attgccgatg agttacatgg cgactacatc
      901 aatgacaaaa gctttaagcg ccattttcag caatggctga actcactgtg gcaggaaaaa
      961 gaccgacttc tgacgtcgtt gatgtcatca cagcggcagg acaagtaaat tttgctcgcc
     1021 tctgaatgag cagaggcgag tgagtatttt aatgaacaaa acgtccggca cgagacataa
     1081 attcttcttc gctcatctcc tgtccgttaa aaacaacttt acccggtgta taacgcagtt
     1141 gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa catctgacct aaggtcatca
     1201 tcccgctgat ttgtttatca gcccgctttt gcgctttttc cgcatccatt ccttcagata
     1261 aattaagctg ttttgcggtt tctgtggcga cattaagcgg taacttcaca tcaaaattga
     1321 gcgatttaat atctttgttt gtggatgatg aagatttggc tgggtcggca atactgatat
     1381 ccagattggc atttagttcg ccgagtgcgt tcttccattt taccggttgt ttaatggtcg
     1441 gctcactttt ttgtaataac ggtaaatact ctttgaacaa tgcagcattc acttcttgca
     1501 gagcaacttc atcgtttgct aactcagggt gtgcagcaag ctgcttctgc attgcaatgt
     1561 tatactggat gataaattgg cgcaccgctg agggatcaat agattcagca atcaatgacc
     1621 actcaccact accgagttgc tgattccctt ttgttaactt atcaagttcg taggtcagat
     1681 tgacattgat ataatctttg tctttaacgc gatccagcat ggtttttgca acgaaagcat
     1741 cgatttgggc aacgtcttcc ccgtgattga taatgttaat ctgattaaat ttcgactcgc
     1801 tttcaccaac cggaaatttc ttttcttcta atcgcgccag agaatcaatg gtaaaacttt
     1861 ttgccgttac tttgttcatt ggtgataatt gcagaacaat atcttcgact ttgccttcaa
     1921 tatccaggtt agacaatgaa ctaccatcac cattaaaagt aaactggccc tcaccaaaag
     1981 aaaactttcc atactctgca ggggcaacat ctaatgtcgt agagaatgag ccaccaaacg
     2041 cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc ggcgataaat agcggttgtg
     2101 tcagttcatt attcactaag ttcacttttg ccgctgccag tgctgggatg acattaccat
     2161 gcattaacat tgtgatgggc aacggaccgt gctcaacatc cacatcaaat acaactttct
     2221 ggcctggctt gatattgaga tcgggtgcac cgttatcgaa tgtcatttgc atttgaaaac
     2281 gagaattaaa aaaacctttg tcaaaatttt tataacttaa cgtcatatcg taggcatgtt
     2341 cacctttctt tttagcatcg ttaaaatctt taataaactt ttcgacacca ggctgaattt
     2401 gcgtacctgt gtaccatgtg ccaccacccc agattacggc taacgcaaca ataacacctg
     2461 tagctgactt acgtatcata taaatattcc ctgataaaac agcaaaactt taaatatccg
     2521 cttatgctga aataatgtcc ag
//
LOCUS       DAJSKJ010000276         2457 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000276 DAJSKJ010000000
VERSION     DAJSKJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.280"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ON345_003826"
     CDS             complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ON345_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR3073802.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT          563 a          709 c          609 g          576 t
ORIGIN      
        1 aaaccgctta ccctgaatat tcagggtaag cgtgagagtt aaaaaagaat tacagcggtt
       61 gggtttgcgc ttcaaccacg gccagcgcca ccatgttgac gatacgacgc accgatgcga
      121 tcggcgttaa cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac
      181 cttccgagct ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca
      241 tcaccagaat attggcagaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg
      301 cttccaccag cgctgcatcg ccgtgcattt caccatcaat catcagttct ggcgcacgtt
      361 ccctgaccag ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa
      421 agttggagtg cgacaacaaa gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg
      481 ccgccatcaa ggtgatctcc gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat
      541 cggcaataaa ggtgttacca ctcggcagca gcagcgcgtt cattgcacct gcggtgtgaa
      601 cgccatcgcg ataaccaaag acatttttca ccacgctaaa atgttcatga taatcgccca
      661 ccgtaccgca gatcattgca tcggcttccc cacgctgaac catgatcgcg ccgatcactg
      721 tcgggttact gatcagcgcc cgctgcgcct gttcctgagt gacaccgcga cgcttcatga
      781 tctggaagta ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgatct
      841 caaaatcaac gcccgctttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt
      901 tcggacgacc gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtggcat
      961 gcagaacgcg cgcctcttcc ccttctggca gaacaacgcg cttcggcgct ttgcgagcct
     1021 gggagaaaat cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga
     1081 tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc cgactccatc gcggctttag
     1141 cgaccgcagg agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt
     1201 ccggaccaaa gctcagatcc tgatcgccat acgctgaagc caccacttcg ctctgttcgg
     1261 cgtgggccag ttccgcaatc gcgcgtaccg ccgccagttt catctcttca ttgatagccg
     1321 ttgcgccaac atccagcgcg ccacggaaga taaacgggaa gcaaaggacg ttgttgacct
     1381 ggtttggata gtcagaacga ccggtgcaaa tgatggcatc cggacgcact tctttcgcca
     1441 gcggcggcag aatttccggt tccgggttcg ccagcgccag gatcattggc gcacgagcca
     1501 ttttcttcac catttcctgg gtcagcactt tcgggccgga acatccgagg aaaatatccg
     1561 cgccttcaat cacatcatcg agggtacgtt tgccgtcatc caccaccgca tatgcggctt
     1621 tggtttccgc catgtttggc tcacggccct gatagataac gccttttgaa tcgcaaacca
     1681 cgatgttatg tttttgcaga cccagcgcta ccagcaggtt catacaggcg attgctgcgg
     1741 cacccgcgcc ggaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaagc
     1801 cgttgaggat ggcggcagtg ctgatgattg ccgtgccgtg ctgatcgtcg tggaataccg
     1861 gaatattcat ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt
     1921 cttcgaggtt gattccgccg aaggtcggtt cgagcgcagc gacaacttca ataaatttgt
     1981 ccgggtcgag ttcatcaact tcaatgtcaa atacatcaat cccggcgaat ttcttaaaca
     2041 gaacgccctt gccttccatc accggtttgc cagccagcgc gccaatgttg cctaacccca
     2101 gcaccgccgt accgttagag atcaccgcca ccaggttacc gcgcgcggta tatttgtagg
     2161 cttttaacgg gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca
     2221 gcgccagatc gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa
     2281 ctggaaattc atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc
     2341 tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc
     2401 acattattac cctgccggag tctacagact ttgagcaagt ccaaactctc accatta
//
LOCUS       DAJSKJ010000277         2437 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000277 DAJSKJ010000000
VERSION     DAJSKJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.281"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            85..495
                     /gene="fliS"
                     /locus_tag="ON345_003827"
     CDS             85..495
                     /gene="fliS"
                     /locus_tag="ON345_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR3073803.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            495..860
                     /gene="fliT"
                     /locus_tag="ON345_003828"
     CDS             495..860
                     /gene="fliT"
                     /locus_tag="ON345_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR3073804.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWEELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            938..2425
                     /gene="amyA"
                     /locus_tag="ON345_003829"
     CDS             938..2425
                     /gene="amyA"
                     /locus_tag="ON345_003829"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR3073805.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
BASE COUNT          642 a          561 c          653 g          581 t
ORIGIN      
        1 catgatgagc aaactgaata acaccagtac ctatctcagc caacaattta ctgcaatgag
       61 taattcctga tcacggaggt cagcatgtat accgcgagcg gctcaaaagc ctatgcacaa
      121 attggcgtcg aaagcgccgt aatgagcgcc agccagcagc agctggtcac catgctattt
      181 gatggagtgc tgagcgcact ggttagagcg cgcctgttta tgcaggacaa caatcagcaa
      241 ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact gcgggtgagt
      301 cttgatgaag agagcaaaga cgaattaacc caaaacttga ttgctcttta tagctatatg
      361 gtcaggcgct tgctgcaagc caatttacgc aacgatgtct ccgcagtcga agaagtggaa
      421 gcattaatgc gtaatattgc cgatgcctgg aaagagtcgt tactctcccc ttctttgatt
      481 caggacccag tctgatgaac catgcaccgc atttgtattt cgcctggcaa caactcgtcg
      541 aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca atgggaagaa ctcatcgcca
      601 gcgaaatggc gtatgtgaat gcggtgcagg agattgcaca tctgactgaa gagattgacc
      661 cgtccaccac gatgcaggag cagctccgcc cgatgctgcg cctgattctc gacaacgaaa
      721 gcaaggtaaa gcagttatta cagattcgga tggatgaact ggcgaaactg gtcggtcagt
      781 catcggtgca aaaatcggtg ttaagtgcct atggcgatca gggcggcttt gtgctggctc
      841 cgcaggataa cctcttttga atctgaacga gtcgatggct cgtgaataat ccgattacgg
      901 ctacgcttct aatgttcccc ttgaatggag tcgaagaatg cgtaatccca cgctgttaca
      961 atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg ccgagcgcgc
     1021 cgacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc
     1081 atcgggcggg tattcggtcg gctacgactc ctatgattta tttgatttag gcgagtttga
     1141 tcagaaaggc agcatcccta ctaaatatgg cgataaagca caactgctgg ccgccattga
     1201 cgctctgaaa cgtaatgaca ttgcggtgct gttggacgtg gtagtcaacc acaaaatggg
     1261 cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgatgacc gtacgcaaat
     1321 tgacgaagaa atcatagagt gcgaaggctg gacgcgttac accttccccg cccgtgccgg
     1381 gcaatactcg cagtttatct gggatttcaa atgttttagc ggtatcgacc atatcgaaaa
     1441 ccctgatgaa gatggcattt ttaaaattgt taacgactac accggtgaag gctggaacga
     1501 tcaggttgat gatgaactcg gtaatttcga ttatctgatg ggtgagaata tcgatttccg
     1561 caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg aacaaacaca
     1621 atgcgacggt tttcgtcttg atgcggtcaa acatattccg gcgtggtttt ataaagagtg
     1681 gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt attgtggcgg agtactggtc
     1741 gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt
     1801 cgatgcgccg ctgcagatga aattccatga agcatcgcgc atggggcgcg actacgacat
     1861 gacgcagatt ttcacgggta cattagtgga agccgatcct ttccacgccg tgacgctcgt
     1921 tgccaatcac gacacccaac cgttgcaagc cctcgaagcg ccggtcgaac cgtggtttaa
     1981 accgctggcg tatgccttaa ttttgttgcg ggaaaatggc gttccttcgg tattctatcc
     2041 ggacctctac ggtgcgcatt acgaagatgt cggtggtgac gggcaaacct atccgataga
     2101 tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg
     2161 tgtacagacg ttatttttcg accatccgaa ctgcattgcc tttagtcgca gtggcactga
     2221 cgaatatccc ggctgcgtgg tggtcatgtc gaacggggat gatggcgaaa aaaccattca
     2281 tctgggagag aattacggca ataaaacctg gcgtgatttt ctggggaatc ggcaagagag
     2341 cgtagtgacc gacgaaaacg gcgaagcgac cttcttttgc aacggcggca gcgtcagcgt
     2401 gtgggttatc gaagaggtga tttaaattca tccccgg
//
LOCUS       DAJSKJ010000278         2380 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000278 DAJSKJ010000000
VERSION     DAJSKJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.282"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            41..529
                     /gene="eco"
                     /locus_tag="ON345_003830"
     CDS             41..529
                     /gene="eco"
                     /locus_tag="ON345_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR3073806.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(678..2324)
                     /gene="mqo"
                     /locus_tag="ON345_003831"
     CDS             complement(678..2324)
                     /gene="mqo"
                     /locus_tag="ON345_003831"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR3073807.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWHLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          554 a          657 c          600 g          569 t
ORIGIN      
        1 catcggtgaa aacctataaa tgaagaagga aagcaaaaaa atgaagacca ttctacctgc
       61 agtattgttt gccgctttcg ctaccacttc cgcctgggcg gcagaaagcg tccagccact
      121 ggaaaaaatc gcgccttatc cacaagctga aaaagggatg aagcgtcagg tgattcagtt
      181 aaccccgcaa gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga
      241 agtcgattgc aatttgcatc gtctcggcgg gaagctggaa aacaaaacgc tggaaggctg
      301 gggctatgat tattatgtct ttgataaagt tagctcgccg gtttccacga tgatggcctg
      361 cccggatggc aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct
      421 gcgttacaac agcaagctgc cgatcgtggt gtatacgcca gacaatgtag atgtgaagta
      481 ccgcgtctgg aaggcggaag agaaaattga caacgcggta gttcgctaaa atgctgtgaa
      541 gtgcggcacc ccgtaggccg aataaggcgg tcacgccgca tccgacatct aacgcccgag
      601 ccggtgcctg atgcgacgct ggcgcgtctt atcagcatac gccacatccg gcataccatg
      661 ccggatgtgg cacatcatta caacgcaata tccgccactt ctttttgaac gggttgcggt
      721 ttcaactgcg gttttggcgt actatctgct gcctgcggct tgtcgtattt cagccccagc
      781 acttcgctgg tgtactgcaa ctcgcgttct gttgccgcta catcaccgtt cagcttgcgt
      841 ccataagacg gaacgatcgc tttcaacgta gcctgccatt gcgggctgga aacgcgatcg
      901 ccaaatactt tttccagcag atccaacata atcggcgcgg cggttgacgc ccctggcgat
      961 gcccccagca gcgcggcaat ggtcccttgc tggtcactga ccacttcagt acccagacgc
     1021 agtacgccac ctttctcggc atcacgcttg ataatctgca cgcgctgccc cgcttgccac
     1081 aaatgccagt cctctttttt tgcctgcgga tagtactctt tcaacgcttc aaaacgatcc
     1141 tcttcactca acatcacctg actcaccaga tatttcacca gatcgaaatt atccagcccg
     1201 acgtgcatca tcggcatcac gttagaggtg gtggtggaac tcatcagatc ccacaatgaa
     1261 ccgtttttga ggaatttggt tgagaaggtg gcaaatggcc caaacagcac tacgcgttta
     1321 ccgtccagaa cgcgggtatc gatatgcgga accgacatcg gtggtgcgcc aacggatgct
     1381 ttaccgtaaa ccttcgccag atggtgatta accacgtccg ggttttccga aacaaggaac
     1441 tgtccgccca ccgggaaacc ggcgtagtct ttcgcttccg gaatccccga ttcctgtaat
     1501 agcttcagcg ccgcaccgcc cgcgccgata aagacaaatt tggcgcggat gttctgtgca
     1561 gtgccgtttt tcagatcggc aacggtaacg gtccaggtgt tatcgtcatt acgctttagg
     1621 gcgcggactt cgctgctgag ttgcagcgag aagttagatt tcttctgtaa ggaagcaatt
     1681 aactggcggg tgatctcgcc atagttcaca tcagtgccaa tttctgtgcg tgttgccgct
     1741 actttctgtt gtggatcgcg cccttccatc actaacggtg cccactcttt gatctgcgcg
     1801 tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg
     1861 gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg aacggtattg
     1921 ataaatgaac gtggagtacg cagcacgccg cgctcaacct ggtgcgccca gaactggcgg
     1981 gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct gccatcggcg
     2041 ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc gttattccag
     2101 ccgttcgaac tctcctgcgc gacaccctcc aggcgctcca ccatggtcat cgaccattca
     2161 ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc gccaatcaac
     2221 agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga aacggcatta
     2281 agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt aatgccttac
     2341 ttttagtcgc tttattgcag gtgagatttg cgcggcatca
//
LOCUS       DAJSKJ010000279         2332 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000279 DAJSKJ010000000
VERSION     DAJSKJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.283"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..773
                     /gene="nirC"
                     /locus_tag="ON345_003832"
     CDS             <1..773
                     /gene="nirC"
                     /locus_tag="ON345_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR3073808.1"
                     /translation="AARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSV
                     RPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMWAILPQTWLGNLV
                     GSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMAL
                     RTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVT
                     LGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            792..2165
                     /gene="cobA"
                     /locus_tag="ON345_003833"
     CDS             792..2165
                     /gene="cobA"
                     /locus_tag="ON345_003833"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR3073809.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          512 a          592 c          651 g          577 t
ORIGIN      
        1 acgctgcgcg cattgcacgc ctgtcggcaa ataacccgct cggcttttgg gtcagctccg
       61 ccatggcggg cgcgtatgtg ggtcttggga tcatcttgat tttcacgctc ggtaatttgc
      121 tcgacccgtc cgtacgtccg ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg
      181 tgattatcgc cggttctgaa ctgttcaccg gacacaccat gttcctcacc tttggggtaa
      241 aagcgggcag catcagccac gggcaaatgt gggcaatcct gccgcaaacc tggctgggta
      301 acctggtcgg ttccgtcttc gttgccatgc tctatagctg gggcggcggt agcctgctgc
      361 cggtagatac cagcatcgtt cactccgtcg cgctggctaa aaccactgca ccggcaatgg
      421 tactgttctt caaaggtgca ttgtgtaact ggctggtttg cctggcaatc tggatggcgc
      481 tgcgcactga aggggcggcg aaatttatcg ctatctggtg gtgtctgctg gcatttatcg
      541 cgtccggcta cgagcactct atcgctaaca tgacgctgtt cgcgctctcc tggttcggca
      601 accacagtga agcctacacg ctggcgggta ttggtcataa cctgctgtgg gtgacgctgg
      661 gtaatacttt atcaggtgcc gtattcatgg gattgggtta ttggtatgct acgccgaaag
      721 cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac ggctgccggt taattactaa
      781 ggggttttta cgtggatcat ttgcctatat tttgccaatt acgcgatcgc gactgtctga
      841 ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt gctgttagac gcaggcgctc
      901 gcttaacggt gaatgcatta gcgtttattc cacagttcac cgcatgggca gatgcaggca
      961 tgttaaccct cgtcgaaggg ccatttgatg aaagccttct cgacacctgc tggctggcga
     1021 ttgcagcgac ggatgatgac gcgcttaacc agcgcgtcag cgaagccgct gaagctcgtc
     1081 gcatcttctg taacgtagtc gatgcgccaa aagccgccag ctttattatg ccgtcgatta
     1141 ttgaccgctc accgctcatg gtcgcggtct cctctggcgg cacctctccg gttctggcac
     1201 gcctgttgcg cgaaaaactc gaatcactgc tgccgttgca tctgggccag gtagcgaaat
     1261 acgccgggca attacgcggg cgagtgaaac aacagttcgc cacgatgggt gagcgtcgcc
     1321 gtttctggga gaaattgttc gttaacgacc gcctggcgca gtcgctggca aacaacgatc
     1381 agaaagccat tactgaaacc accgaacagt taatcaacga accgcttgac catcgcggtg
     1441 aagtggtgct ggttggtgca ggtccgggcg atgccgggct gctgacgctg aaaggactgc
     1501 aacaaattca gcaggcagat gtggtggtct acgaccgtct ggtttctgac gatattatga
     1561 atctggtacg ccgagatgct gatcgcgttt tcgtcggcaa acgcgcggga taccactgtg
     1621 taccgcagga agagattaac cagatcctgc tgcgggaagc gcaaaaaggc aaacgcgtgg
     1681 tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg tggcgaagag ctggaaacac
     1741 tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat taccgcagct tctggttgct
     1801 ctgcctattc gggtattcca ctcacgcatc gcgattatgc ccagagcgta cgcttaatta
     1861 ccggacactt aaaaaccggt ggcgagctgg actgggaaaa cctggcggca gaaaaacaga
     1921 cgctggtgtt ctatatgggg ttgaatcagg ccgcgactat tcagcaaaag ctgattgaac
     1981 acggtatgcc tggcgaaatg ccggtggcaa ttgtcgaaaa cggaacggca gtcacgcagc
     2041 gcgtgattga cggtacgctc acgcaactcg gtgaacttgc tcagcaaatg aacagtccat
     2101 cgctaattat tattggtcgg gttgttggcc tgcgcgataa actgaactgg ttctccaacc
     2161 attaatttaa cccgggccag agaattctgg ccttcttaat gactcttttt atatattcag
     2221 caaataaaac ataacccctt aataaatatt atctgctaat gggttatatt caccgttaca
     2281 gcgacttaca aaattaagtc attcgctcac tgacgccaat taaatattcc tg
//
LOCUS       DAJSKJ010000280         2310 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000280 DAJSKJ010000000
VERSION     DAJSKJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.284"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(33..983)
                     /gene="cbl"
                     /locus_tag="ON345_003834"
     CDS             complement(33..983)
                     /gene="cbl"
                     /locus_tag="ON345_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR3073810.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFVRKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1085..2002)
                     /gene="nac"
                     /locus_tag="ON345_003835"
     CDS             complement(1085..2002)
                     /gene="nac"
                     /locus_tag="ON345_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR3073811.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT          624 a          586 c          500 g          600 t
ORIGIN      
        1 ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca
       61 ctgttttcca tcacctggcg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc
      121 agaaagcgcc agacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta
      181 ttagcatcga aaagatgccg cgtatccagg cggattaaat tctcttcctc ttgttcgccg
      241 ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca
      301 tcagaatcct gtgcacttaa tacaatatct gccagcaaac ctttgcggac aaatgcgtca
      361 tcaatacgtg agcgccccgt aatcccctgt cggtaagtaa ttaacggcca cttcgctatt
      421 gattccagcg tcaacggtgt aatttgcgtc aagggatgat cgagtggaac aagcaaacta
      481 tggtgccaac gaaaccacgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg
      541 gcaataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg tggcgtaccc
      601 tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc
      661 aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg
      721 gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg ttctgcaatg
      781 accagtaatg ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat
      841 atttcgatgc caagttcatc ctccagttcc cgaatatgac ggctaacgcc tgactgtgag
      901 gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt
      961 atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatatcc cgttattgtt
     1021 aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata
     1081 acgcttagct caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca
     1141 ttaacaactc tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta
     1201 aggacaaaga gagactcatt gaaggagtgg taatgcgtga catccaccca tttactgcgc
     1261 cacataacga acgcgcagcc gattcaggta atactgcaac gcccatgccg ctggcaatcg
     1321 ctgcggtaag cgtggcaata gactcaattt caccaatgac ttttgccgtg agccgccgta
     1381 gggaaaaagc ctcatcaaca cgaagtctaa cagcgctgta atcactgggg agaaaaaggt
     1441 tcatttgcgc aatagcatta acatcaacgt tttgccccgg gcaatcttga gttcctacca
     1501 ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg gaatgctcgt
     1561 aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact gcaccactat
     1621 tttcatgaag atagataacg acctccggga attcagcgcg aaccgcctgt aataagggca
     1681 tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg
     1741 actgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgtg
     1801 catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca
     1861 aaagttgttg atttaactca ccttccagtg tggcaacctg ctggctgagc gctggttgtg
     1921 cgatatgcaa tacttcagca gcctgagtca ggctaccaat atctacaatt tttacgaagt
     1981 atttcaggcg tctgaagttc atgttgcctc cggtttttaa gagtcggccc aagtgccgcc
     2041 attacttaca accagaatgc aagatgcttg ccagttctat tttggcgttg atgcgcaagc
     2101 taaccaactg tcaaataaga gattatgata gattcgtcat tggctctttt tatctgcagt
     2161 cgcattcacc tgccctataa aaggagggta tgcaccacga tggttcatta cccaataaga
     2221 ttgaaagctc accactttgt tgaaattggc agcaaacaaa caaaaaaatg catttcaccc
     2281 tttgacatca ccatgcactg ccattaatat
//
LOCUS       DAJSKJ010000281         2292 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000281 DAJSKJ010000000
VERSION     DAJSKJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.285"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            91..594
                     /locus_tag="ON345_003836"
     CDS             91..594
                     /locus_tag="ON345_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3073812.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHINYALGCTGHVDCE
                     VRMLREL"
     gene            complement(640..1056)
                     /gene="rraB"
                     /locus_tag="ON345_003837"
     CDS             complement(640..1056)
                     /gene="rraB"
                     /locus_tag="ON345_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR3073813.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1218..2222
                     /gene="argF"
                     /locus_tag="ON345_003838"
     CDS             1218..2222
                     /gene="argF"
                     /locus_tag="ON345_003838"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3073814.1"
                     /translation="MSRFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          587 a          543 c          575 g          587 t
ORIGIN      
        1 aactttatta ctttataagt cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt
       61 ataattactt tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta
      121 atgcgtcgcc tgacgcagca ggataatccc gctatcgccc gcgtcattcg tcaggtatcc
      181 gccgaatacg gtcttaccgc tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag
      241 ctgtatcaag tgtatagtca gcctggccat gcatattggg tggttgagta cgagggtgaa
      301 gtggtcggcg gcggtgggat tgcgccatta accgggagtg aatcggacat ttgcgaactg
      361 caaaagatgt attttcttcc ggctattcgc ggtaaagggc tggcgaaaaa actggcctta
      421 atggcgatgg agcaggcacg agagatgggc ttcaaacgct gctatctgga aacgaccgct
      481 tttttaaagg aagccattgc gctttatgag catttgggct ttgagcatat caactatgcg
      541 cttggctgca cgggccatgt cgattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat
      601 gcccgctagt gtgtgctatc gggcatttta atcaattaat tagtggcgaa caccgtcatc
      661 gtcttcatcg acaaaatctt catcgtcgcc gtcttcgcca ttgggatctt caaagtaagt
      721 gccccaaccg tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg
      781 ggcatcgatc agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat
      841 atcaccatct tcaacttcca gctcttctgg atcggtcact tcgtaaccga gtttaaacgc
      901 ttcaactgct gctttttcca gggtttccag atcgtctgcg gaaagatggt gttcgatggt
      961 gtacagcgcg tccggatcgc tgccatcttc cagtaattct tcaataatca aacgtgtttc
     1021 ttcacgctgt tcttccagtt gttccgggtt tgccatggct cattcctctt aatgtcctgc
     1081 cgatactctt attgtcacac accactgaca ttgcctccac ctttgtgaca aagatttatg
     1141 ctttagactt gcaaatgaat aatcatccat ataaattgaa ttttaattca ttgaagcttt
     1201 agccacagga gggatctatg tccaggtttt atcataagca tttcctgaaa ttactcgatt
     1261 tcacgccagc tgaactcaac agcctgctgc agttagccgc gaagctgaaa gccgataaga
     1321 aaagcggtaa agaagaagcc aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag
     1381 actcgactcg tacccgatgc tctttcgaag ttgccgcata tgaccagggt gctcgtgtta
     1441 cttatctcgg cccaagcggc agccagattg gtcataaaga gtcgattaaa gacactgccc
     1501 gcgtgcttgg tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg
     1561 aaacactggc ggaatacgct ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc
     1621 ccacgcagct gctggcggat cttctcacca tgcaggagca tttgcccggt aaagcgttca
     1681 acgaaatgac gctggtctat gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg
     1741 aagctgcggc gcttaccggt ctggatttgc gtctggtcgc gccacaggca tgctggccgg
     1801 aagctgcgct ggttacggaa tgccgcgccc tggcacagca aaatggcggg aatattacgc
     1861 tgacggaaga tgtagccaaa ggagttgaag gtgctgactt tatctatacc gatgtatggg
     1921 tgtcgatggg ggaagcaaaa gagaaatggg cagaacggat tgcattactg cgtgattatc
     1981 aggtgaacag caagatgatg cagttgaccg gtaacccgga ggtcaaattc ctccactgcc
     2041 tgcccgcgtt tcatgacgac cagacgacgc ttggtaagaa aatggcggaa gagtttggcc
     2101 tacatggcgg aatggaagtg actgatgagg tcttcgaatc tgccgccagc attgtttttg
     2161 atcaggcgga aaaccgcatg cataccatca aagcggtgat ggtcgcgacg ctcagtaaat
     2221 aattgatatc gctataaaga tgccgggaaa tacaaaaaca tcccggcatc acattatttt
     2281 atgcagtaat ag
//
LOCUS       DAJSKJ010000282         2263 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000282 DAJSKJ010000000
VERSION     DAJSKJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.286"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            223..2220
                     /gene="betT"
                     /locus_tag="ON345_003839"
     CDS             223..2220
                     /gene="betT"
                     /locus_tag="ON345_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR3073815.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMVDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
BASE COUNT          501 a          551 c          582 g          629 t
ORIGIN      
        1 atctcaatgc aagagatggc aatattatgg gggcacggta ttatcaacct cagcccctaa
       61 aaacctcttt ccgatgctac tctacaaccc cctaaaaaac cggtggcgat tcacatccca
      121 ccatgcattc cgattaacgc cgcatagcca gttgaacttt gctactctgt gagaggtagt
      181 accttctatc cagtgcgaat ttaattaatg gaataaatga ttatgagtga aaatgataca
      241 atcccaaaga agtctacaag tcagattaac aaagcggtat tctttacatc tgctttgcta
      301 attttccttc ttgtcgcctt tgccgccgta ttcccggatg tcgccgacaa aaattttaaa
      361 ctacttcagc aacaaatctt cacgaatgcc agctggttct acatccttgc tgtggccctg
      421 attttactga gtgtcacgtt ccttggactc tcacgctacg gtgatatcaa gctgggcccg
      481 gaccatgcgc agcctgattt cagctaccac tcctggtttg cgatgctttt ttcggcaggg
      541 atggggatcg gcctgatgtt ctttggcgtt gccgaacctg taatgcatta tctttcgcca
      601 cccgttggca ctccagaaac cgttgcggca gccaaggaag caatgcgtct gacctttttc
      661 cactggggac tgcacgcatg ggcaatttat gccattgtgg cgctgattct ggcgttcttc
      721 agttaccgtc acggtctgcc tttaactctg cgctccgcac tctatcccat tattggcgat
      781 cgcatatacg gacctgtagg acatgcggtt gatattttcg ctgttatagg cacggtcttt
      841 ggcgttgcga catcactggg ttacggtgtt ttgcaggtga atgccggttt gaaccatctt
      901 ttcggggtgc ccatcaatga aacggtgcag gtaattctga tcgtggtcat cacggggtta
      961 gcgacgattt cagtggtgtc cggtctggat aagggaatac gtatcctgtc tgaactcaat
     1021 ctgggtctgg ctttgttgct cctggcgctg gtcctgtgtc tgggaccaac cgtgcttctg
     1081 ctgaagtcat ttgtggaaaa tacgggcggt tatctttcgg aactggtgag taaaacgttc
     1141 aacctttacg cgtatgagcc caagtcgagc aactggctgg ggggctggac attactgtac
     1201 tggggatggt ggctttcatg gtcgccgttt gtggggatgt tcatcgcacg ggtctcccgc
     1261 gggcgaacca ttcgcgagtt tgtcaccggc gtgctgtttg ttcccgcggg ttttacgcta
     1321 atgtggatga cggtgtttgg taacagcgcg atctatctca ttatgaacca gggggccaca
     1381 gacctcgcca atactgttca gcaggatgta gcgctggccc tgtttaattt cctggagcat
     1441 ttcccgttct cttccgtgct gtcattcatt gcaatggcga tggtcatcgt cttctttgta
     1501 acgtctgctg attcgggggc aatggttgtg gatactctgg catcaggtgg agtggcaaac
     1561 acacccgtct ggcagcgaat attctgggcc tcgctcatgg gcattgttgc aattgcgctt
     1621 ctccttgccg gagggctaag tgcgctgcaa acggtgacaa tagcgagtgc attgcccttc
     1681 tcagtgatct tactaatatc catatacgga cttttaaaag ctttgcgccg ggatttgacc
     1741 aagcgtgaaa gcctgagcat ggcgacaatt gctcctacgg ctgcacgtaa cccaattcct
     1801 tggcagagaa ggttacgcaa tatcgcgtat ctgccgaagc gatctcttgt gaaacgtttt
     1861 atggtcgacg tcatccagcc cgccatgacg ctggttcagg aggaactgaa caagcagggg
     1921 acgataagcc acattagtga tgcagtcgac gatcgtattc gtcttgaagt cgatttgggc
     1981 aacgagctga attttatata tgaagtgagg cttcgcgggt atatctcacc gaccttcgcg
     2041 ctcgccgcaa tggataatga tgagcagcag actgaacaac atcgatatta tcgcgctgag
     2101 gtttatctca aagaaggcgg tcaaaattat gatgtgatgg gctggaacca ggaacagttg
     2161 attaatgaca tactggacca gtacgaaaaa cacctgcact tcctgcacct ggttcgttaa
     2221 tagcaacatg ccgtcctggg ggcggcaatt attatcccgg ccg
//
LOCUS       DAJSKJ010000283         2224 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000283 DAJSKJ010000000
VERSION     DAJSKJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.287"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            91..630
                     /locus_tag="ON345_003840"
     CDS             91..630
                     /locus_tag="ON345_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR3073816.1"
                     /translation="MTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLL
                     RFRHMLACRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWM
                     ASHKPDLVIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQ
                     VLVDAEKAITDFMTARGYH"
     gene            complement(679..2178)
                     /gene="pitB"
                     /locus_tag="ON345_003841"
     CDS             complement(679..2178)
                     /gene="pitB"
                     /locus_tag="ON345_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR3073817.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          589 a          578 c          545 g          512 t
ORIGIN      
        1 gtgtgtgaac atcttattac cgttgtcgaa aaatatggtg ctgccgaaag agttcattta
       61 ggaaaacagg ccggaaatgt cggtcgtgca gtgaccaaat taccgttgat gggaaaatcc
      121 ttacataaaa caattgaacg aaatcaggtg aaaacagcaa aaaaattgcc tggaccagtt
      181 ccggcgctgg taattacagc gtttgtcgcc cgtcgcttac tgcgctttcg tcatatgctt
      241 gcctgtcgtc gtcgcgggtt aattgttcta accgaccgtt atcctcagga ccaaattcct
      301 ggcgcttacg atggtacggt gttcccacct aacgttgaag gtggtcgttt tgtctcatgg
      361 ctggcaagcc aggaacgtaa agcgtttcac tggatggcga gccataagcc tgatctggtc
      421 atcaaactca atgttgacct tgaagttgcc tgtgcacgta aacccgacca taaacgggaa
      481 tcgctggcga ggaagattgc cataacgcca cagttaacct ttggtggtgc acaactggtt
      541 gatatcgatg ccaatcagcc actggaacag gtgttagttg atgcagaaaa agcgattacg
      601 gattttatga ccgcgcgtgg ttatcactag ccaaaaatgg aaatgcccga tcgccaggac
      661 cgggcatttt caggaaggtt aaatcaactg caatgctatc cagtacagcc caccagaaag
      721 aaaaatcgcc gccggtaaag taaataccca cgccatcagg atgctggtta ccgttttacg
      781 ctgtaaccca ccgccgtcca ccaccatcgt ccctgcaact gcagacgaga ggacgtgtgt
      841 tgtggagacg ggcatcccaa tataactggc aagaccgata gacactgccg ccgtcatttg
      901 tgccgccatg ccttgcgcat acgtcatgcc gcgcttacca atcttctcac cgatggtcat
      961 cgctacacga cgccagccaa tcatggtgcc aatgccgagc gccagtgcta ccgccatgat
     1021 gatccacacc ggagcgtact caatggtgct taacatatcg ctgcgaagtt ttttcagcag
     1081 gttctggtct tctttactga cgcctggcag tttcgctagc ttcgcggagg tatcagagat
     1141 gcacagcata atgcggcgca gctggctgcg ctgactcacg cttaacggct cgtaactttc
     1201 catattgcct ggcagcatcg ttttaacgcg cgcaatcgca tcaaaggtat ttgccggatg
     1261 acagtgaaac tctgttactt gcgtgccatc agtcgatgct gcaggcaatg gaggttccat
     1321 cgcaatcaac ttctgcggca gttcaggatg ctgttgcagg tagtgttcga agttggtaac
     1381 ggcatcgcgg gtacgggtaa tttcatagcc ggacgcattc atattgacga cgaagccagc
     1441 aggggcaatc cccaccagta ccagcattac caggccgatc cctttttgtc cgtcgttcgc
     1501 gccgtgcgaa aacgccacgc ccgcagcgga aacaatcagc gcaatacgcg tccagaatgg
     1561 cggtttacgt ttgccttttt tctttttgcg atcttccgga atgcggtgaa tacggtcacg
     1621 ctttttcgtc ccgctccagt agcgtcgcag caggaatatc aggcctcccg caatgaccag
     1681 gccgacgata ggggaaacaa tcagcgagga gaaaattttg gtcacttcac gcaggtttaa
     1741 cgcatccatc accgatgagc cggttaacag cgcgttggtt aaaccgatgc cgataatcgc
     1801 accaatcaag gtgtgcgaac tggaggccgg taaaccgaag aaccacgttc ccaggttcca
     1861 gataatcgcc gccagcagca tggaaaagac catcgccagg ccgtgggttg accccatatt
     1921 cagcaacaaa tcggttggca acatatggac aatggcatag gcaacgctaa gtccgcccaa
     1981 taacacgcca aaaaagttaa aaaatgccgc catcaccaca gcaagttgtg gttgcatggc
     2041 acgagtataa ataacggttg ccaccgcatt cgccgtgtca tgaaaaccat tgattgcttc
     2101 gtagaacaac acaaatgcca gagcaagcaa taacaaaagc cctgtgtata tatcaaggcc
     2161 aacaaataaa tttagcatat attagattac gccattttga atttacgaac ggacgcatta
     2221 tcag
//
LOCUS       DAJSKJ010000284         2164 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000284 DAJSKJ010000000
VERSION     DAJSKJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.289"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..245)
                     /gene="prlF"
                     /locus_tag="ON345_003842"
     CDS             complement(<1..245)
                     /gene="prlF"
                     /locus_tag="ON345_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCR3073818.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNP"
     gene            complement(394..1965)
                     /gene="garD"
                     /locus_tag="ON345_003843"
     CDS             complement(394..1965)
                     /gene="garD"
                     /locus_tag="ON345_003843"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR3073819.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
BASE COUNT          504 a          577 c          527 g          556 t
ORIGIN      
        1 tgcgggttgt tctggatatc tgcatccaga aaacgcaaaa atgcattcat agtatgatcc
       61 tcctgttcat ctcccagtcg gcacataaat acttgcccac caggcagaat ttcgtaatga
      121 atgctgtcct ggcctggctt cagttttaag gcctcacgca ctggtgcggg gatagttgtt
      181 tgtccgcgta tcgtgacctt tgattcagtg gtcagtacag cgtgagagcg agcattagcg
      241 ggcatgattc actgtccttt tacagcctgt tttctgctca aattataagc ttgaactaag
      301 gtaatgcaaa tgcattatta atggacgtgg gggctttaaa caataaaaac atggcactaa
      361 ggccatgttt tgcgagggac gttccaaaag aaatcaggtc accggtgccg ggttaaacac
      421 cgccagctgg ttatgcagtc cccattgatc cgagaaggtt ttcttcttcc cgctggcgac
      481 gtcgagaata aagtggaaca acttccagcc cacctcttca atagtttctt cgccggtagc
      541 gatggtgccc gcattaatat ccattaaatc aaaccagcgg ttcgccagct cggtgcgggt
      601 tgccatttta atgacgggta ccgccatcag gccgtacggc gtaccacgac cggtcgtaaa
      661 cacttgcact gtgatacccg aagccacctg ttgcgtgcca cagacaaaat cgctggctgg
      721 cgtcgcggcg taaattaatc cgcgtttagt cgggcgttga ccgggcgaca gcacttcaac
      781 aattgcgctt ttacccgatt tagcaatgga gccgagtgcc ttctctacca cgtttgccag
      841 accgcctttc ttgttgcccg gcgaagggtt ggcgctgcgg tcggtttttc ccatattgag
      901 atagttatcg taccactcca tctcctccag cagccgtttg ccgacctctt cgttcactgc
      961 gcgtggtgtc agcagatgga tcgcgtcacg cacttccgtt acttctgaaa acatcaccgt
     1021 tgcgccgcag cgcaccagta gatcagacgc atagccaacc gccgggtttg ccgttacacc
     1081 agaaaacgca tcgctgccac cgcactgcat accaacgacc agttctgaag ccgggcaggt
     1141 ttctcgctgc cgttgattca gtttttgtag atggcgttcg gcgatctgca aaatatcctc
     1201 gaccatggac tgaaaaccga catgcttttc atcctgcaag ctgacaatac tggcgttctc
     1261 cagcggaata ccttgtacat catccgtgcc gaccagcagg cgttctggct gtaatttctc
     1321 gcaacccagg ccaatcacca tcacctcgcc gccaaagttt gggttaagcg aaatgttgtg
     1381 aatggtccga atgggcacca ccgccgccgg agcgttaatc gccacgccac aaccgtacag
     1441 atgattcagc cccaccacgc catcgacgtt cgggtatttc ggcagcagat cgcgttcaat
     1501 gatttttact acgtagtcca ccacgcctgc cacacagtgg acgctggtgg tgatgccgag
     1561 caggtttttg gtgcccacgc tgccatcggc attgcgatag ccttcaaagg tgtaaccttc
     1621 cagcggcggt aagggttccg ggactttggt tgccagtggc agcgtgtgta acggcggcgc
     1681 ttccggtagt acaaccattg attcgtcgat ccagcttccg cgtgggattg cgcgcacggc
     1741 gtaaccaatc acttcgccat aacgaataat ttcaccatta gccggaatgt ccagcaatgc
     1801 gactttatgc ccctggggaa tatgttcaat taattccagc ccatccggaa aacgcgttcc
     1861 tgctttcagg ccattatcat taacaataat tgccacatta tctgtgtcgt gaacttttat
     1921 ataaaacgca gttggcgttt cttgtctgat ttcgatgttg gccattgtgc aatattctcc
     1981 agccaggggt tattaaaatg aggacctggt ctcatttaaa aataaatgac ttcattgggt
     2041 gaaataagaa tgtcaggagt ttaaacgcag aaatgtgatc aatgtcaatc atttcaggga
     2101 ttgcagtgcc gcccggcaag gtaaagcgca aatctgtgct ttagcgcgca accttatgtg
     2161 cata
//
LOCUS       DAJSKJ010000285         2095 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000285 DAJSKJ010000000
VERSION     DAJSKJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.290"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..180)
                     /locus_tag="ON345_003844"
     CDS             complement(<1..180)
                     /locus_tag="ON345_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3073820.1"
                     /translation="MDLNTAANALRELGHPTRLSIYRELVRAGHEGLPVGELQKHLEI
                     PASTLSHHLSALISAG"
     gene            429..1142
                     /locus_tag="ON345_003845"
     CDS             429..1142
                     /locus_tag="ON345_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017349110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3073821.1"
                     /translation="MDLRPLNELTEILGVSHMTIRRDVSKLEEQGLLVSVSGGVRAVS
                     RLAAEPSHLVKSTLQSEEKQAIGALAASHIAKNSCIYLDAGTTTLALARAILDRNDLQ
                     VVTNDFEITQLLIDASQCGVIHTGGTLCRENRSCVGESAARTLRHLAIDTAFISASGW
                     DSRGIFTPDENKVTVKETVSQVSARSILLCDSSKYNQVATFMALPLTRFTTIITDRHL
                     SDAAASHIARHACEVLRAG"
     gene            complement(1163..2023)
                     /locus_tag="ON345_003846"
     CDS             complement(1163..2023)
                     /locus_tag="ON345_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase SHV-12"
                     /protein_id="HCR3073822.1"
                     /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIE
                     MDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYS
                     PVSEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDR
                     WETELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIR
                     SVLPAGWFIADKTGASKRGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGI
                     GAALIEHWQR"
BASE COUNT          423 a          625 c          623 g          424 t
ORIGIN      
        1 tccagcggat atcagcgctg aaagatgatg gctgagcgtg gaggcaggaa tctcaaggtg
       61 cttctgcagt tcgccaaccg gcaggccttc atgacccgcc ctgaccagct cccggtatat
      121 gctgagacgt gtcgggtggc ccagttctcg aagtgcgttt gcagcagtgt ttaaatccat
      181 aatacctcct ttatctatat ttccagaata atagaaatat agcctgcgtc aatcgtttct
      241 gccgtgaggg taccgctttc ccaatcatgc tctgtccgcc aggttatggg gggcagagcc
      301 agagcactac cgaaatcctt gcgaataccc gtgcagggaa taatttccgc cccctttatg
      361 gccggaaaaa aaactgataa acgtcgcctt ttcagaaggt tgctttctta acgtggtttt
      421 tcgcatggtt ggacctcaga ccccttaacg agctgacgga gatcctcggc gtgtcccata
      481 tgaccatccg tcgggacgtc agtaaactgg aggagcaggg gctgctggtc agcgtctcgg
      541 gcggcgtacg cgccgtcagc cggctggccg cggaacccag tcatctggtg aaaagcacgc
      601 tgcagagtga ggagaaacag gcgatcggcg cgctggcggc gagtcatatc gctaaaaaca
      661 gttgcatcta tctggatgcc gggactacca ccctggcgct ggcgcgggcg atcctcgacc
      721 ggaacgatct ccaggtggtc accaatgatt ttgagataac ccagctgctg atcgatgcca
      781 gccagtgcgg cgtgatccac accggcggca ctctgtgccg ggagaaccgc tcctgcgtgg
      841 gcgaatcggc ggcgcgtacg ttacgccacc tggctatcga tacggccttt atctccgcca
      901 gcggctggga cagtcgcggg atattcaccc ctgatgagaa caaggttacc gtcaaggaga
      961 ccgtcagcca ggtcagcgcg agaagcatcc tgctgtgcga cagttcgaaa tacaatcagg
     1021 tggccacgtt tatggcgtta cctttgaccc ggttcaccac catcattacc gaccggcatc
     1081 tttccgatgc cgccgccagt catatcgccc ggcacgcctg cgaggtgctg cgggccggat
     1141 aacgcgcgcg gccaccgccg gcttagcgtt gccagtgctc gatcagcgcc gcgccgatcc
     1201 cggcgatttg ctgatttcgc tcggccatgc tcgccggcgt atcccgcaga taaatcacca
     1261 caatgcgctc tgctttgtta ttcgggccaa gcagggcgac aatcccgcgc gcaccccgct
     1321 tgctagctcc ggtcttatcg gcgataaacc agcccgccgg cagcacggag cggatcaacg
     1381 gtccggcgac ccgatcgtcc accatccact gcagcagctg ccgttgcgaa cgggcgctca
     1441 gacgctggct ggtcagcagc ttgcgcaggg tcgcggccat gctggccggg gtagtggtgt
     1501 cgcgggcgtc gccgggaagc gcctcattca gttccgtttc ccagcggtca aggcgggtga
     1561 cgttgtcgcc gatctggcgc aaaaaggcag tcaatcctgc ggggccgccg acggtggcca
     1621 gcagcagatt ggcggcgctg ttatcgctca tggtaatggc ggcggcgcag agttcgccga
     1681 ccgtcatgcc gtcggcaagg tgtttttcgc tgaccggcga gtagtccacc agatcctgct
     1741 ggcgatagtg gatctttcgc tccagctgtt cgtcaccggc atccacccgc gccagcactg
     1801 cgccgcagag cactacttta aaggtgctca tcatgggaaa gcgttcatcg gcgcgccagg
     1861 cggtcagcgt gcggccgctg gccagatcca tttctatcat gcctacgcgg cccgacagct
     1921 ggctttcgct ttgtttaatt tgctcaagcg gctgcgggct ggcgtgtacc gccagcggca
     1981 gggtggctaa cagggagata atacacaggc gaatataacg cataaccaca atacatcctt
     2041 gagtgaaggc cgataaaggc gagtaaagaa gcgacaaata agaataaccc ggcgt
//
LOCUS       DAJSKJ010000286         2084 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000286 DAJSKJ010000000
VERSION     DAJSKJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.291"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..245
                     /locus_tag="ON345_003847"
     CDS             <1..245
                     /locus_tag="ON345_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR3073823.1"
                     /translation="WVKTQPCACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKH
                     HNELHTDTVAFEEKYGSQLELIFRFIDRALATGVLA"
     gene            259..1011
                     /locus_tag="ON345_003848"
     CDS             259..1011
                     /locus_tag="ON345_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR3073824.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMLQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(1186..1388)
                     /gene="cspF"
                     /locus_tag="ON345_003849"
                     /pseudo
     CDS             complement(1186..1388)
                     /gene="cspF"
                     /locus_tag="ON345_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            1687..1902
                     /gene="cspB"
                     /locus_tag="ON345_003850"
     CDS             1687..1902
                     /gene="cspB"
                     /locus_tag="ON345_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HCR3073825.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT          601 a          381 c          524 g          578 t
ORIGIN      
        1 gttgggttaa gacacagccg tgtgcttgct gcggtatgcc agccgacgat ccgcatcatc
       61 tgattggtca cgggcagggc ggaatgggaa caaaagcaca tgatctcttt gtgttgcctt
      121 tgtgcagaaa gcatcacaac gagcttcata cggatacagt ggcatttgaa gagaagtatg
      181 gctcccaact ggagctgata tttcgtttta tcgatcgcgc gctggcaact ggcgtactgg
      241 cgtaagtgga gaacgagcat gaaccttgaa gccttaccaa aatattactc cccaaaatct
      301 ccaaaattga gcgatgacgc tccagcgaca ggcaccggtt gtttaacaat tacggatgtg
      361 atggctgcgc aggggatgtt gcagtcgaaa gcaccacttg ggttggcctt atttctggca
      421 aaagttggtg ttcaggaccc tcagtttgcg attgaaggcc tgctaaatta cgcgatggca
      481 ctggataacc cgacattgaa caaattgagt gaagaaatcc ggttacagat tattccttac
      541 ctcgtgaatt ttgcctttgc tgattactcc aggtctgcgg caagtaaggc tcgctgtgag
      601 cattgttcag gtacgggatt ttataatgta ttgcgcgaag tggtgaaaca ctacagacgc
      661 ggggaatctg taatcaagga agaatgggta aaggaactat gtcagcattg ccatggtaag
      721 ggcgaagtca gcacagcgtg cagagggtgt aagggtaaag ggattgttct ggatgaaaaa
      781 agaacccggt ttcatggcgt accggtatat aagatttgtg ggcgttgtaa tggaaaccgg
      841 tttagtcgtt taccgaccac gctggcacga cgtcatgtcc agaagctggt accagacctg
      901 accgattatc agtggtataa ggggtatgcg gacgtcattg gtaaactggt aacaaagtgc
      961 tggcaggaag aagcatacgc ggaagcgcaa ttgaggaagg tgacgagata aatgattttt
     1021 gctgaagatg gcgacatgat gtttgcattt ttcaaaaaac atggataaga ttttctcaac
     1081 gatgggcttt gtgtatccga cgtttagaaa aaagtagaaa acccgcttat aagcgggttt
     1141 ttgtgcttta aatggggcaa tagagatatt gaatctcatc ccgggataaa cattggcagt
     1201 tgaaggtcca cgcgaaccat ttatccggca aaattccacg cgtaatcctg tggtaatttc
     1261 ttctgcatct cgaagattga gagctgaaac gtgaagctgg gcatcgatac accatcggat
     1321 ggggatataa gacctttgct gcttttgtag tcaaagtttt tgacaattcc tgtcatttca
     1381 ggggacagaa aaactcctta atactgataa cctggtgcac catacacacg ttcctggaga
     1441 aaactacttt tttgataggg ttgaaggtgg ctggatgtct aaaataaaca ttgcttcata
     1501 tgttcaacta tgagttaatg acttcgtcgg tttgaagaac agacgatata cgaagtagtt
     1561 tactaaagca gttctcattt caggtgttat tcacttattc cttctttgag tctctccaat
     1621 taagtacgaa gtcgtttctg ttatgcaaac catttatgcc gaaaggctca agttaaggaa
     1681 tgtagaatgt caaataaaat gactggttta gtaaaatggt ttaacgctga taaaggtttc
     1741 ggctttattt ctcctgttga tggtagtaaa gatgtgtttg tgcatttttc tgcgattcag
     1801 aatgataatt atcgaacctt atttgaaggt caaaaggtta ccttctctat agagagtggt
     1861 gctaaaggtc ctgcagcagc aaatgtcatc attactgatt aaaattcatc gctcgtctgt
     1921 atacgataac gaagaaggct gatgcctgag tagagatacg gacagagtag tgaatattgg
     1981 atctctttaa taaaaattaa ggaggtccaa tacatgaaac aatggccagc atatttggca
     2041 aaatcctaat caggaaaagt atgctaacca ttgtggtgaa gtgc
//
LOCUS       DAJSKJ010000287         1969 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000287 DAJSKJ010000000
VERSION     DAJSKJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.292"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            140..1105
                     /gene="dusB"
                     /locus_tag="ON345_003851"
     CDS             140..1105
                     /gene="dusB"
                     /locus_tag="ON345_003851"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR3073826.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELNDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1131..1427
                     /gene="fis"
                     /locus_tag="ON345_003852"
     CDS             1131..1427
                     /gene="fis"
                     /locus_tag="ON345_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR3073827.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1513..1905
                     /locus_tag="ON345_003853"
                     /pseudo
     CDS             1513..1905
                     /locus_tag="ON345_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
BASE COUNT          527 a          464 c          498 g          480 t
ORIGIN      
        1 taattatcgc accacgctca ttttaaatgc aattctttga tccatctcag aggattggtc
       61 aaagtttggc ctttcatctc gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag
      121 tatggtcatt tcttaactca tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc
      181 agcgcccatg gctggcatta cagacagacc ttttcggacg ttgtgctacg agatgggagc
      241 cggattgaca gtatccgaga tgatgtcttc taacccacag gtttgggaaa gcgacaaatc
      301 tcgtttacgg atggtgcaca ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag
      361 cgatccgaaa gaaatggcag atgcagcacg tattaacgtg gaaagcggtg cccagattat
      421 tgatatcaat atgggttgcc cggctaaaaa agtgaatcgc aagctcgcag gttcagccct
      481 cttgcagtac ccggatgtcg ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt
      541 tcctgttacc ctgaagattc gcaccggctg ggcaccggaa caccgtaact gcgaagagat
      601 tgcccaactg gctgaagact gtggcattca ggctctgacc attcatggcc gtacacgcgc
      661 ctgtttgttc aatggagaag ctgagtacga cagtattcgg gcagttaagc agaaagtttc
      721 cattccggtt atcgcgaatg gcgacattac tgacccgctt aaagccagag ctgtgctcga
      781 ctatacaggg gcggatgccc tgatgatagg ccgcgcagct cagggaagac cctggatctt
      841 tcgggaaatc cagcattatc tggacactgg ggagttgctg cccccgctgc ctttggcaga
      901 ggttaagcgc ttgctttgcg cgcacgttcg ggaactgaat gacttttatg gtccggcaaa
      961 agggtaccga attgcacgta aacacgtttc ctggtatctc caggaacacg ctccaaatga
     1021 ccagtttcgg cgcacattca acgccattga ggatgccagc gaacagctgg aggcgttgga
     1081 ggcatacttc gaaaattttg cgtaaacaga aataaagagc tgacagaact atgttcgaac
     1141 aacgcgtaaa ttctgacgta ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc
     1201 aaaaacccct gcgtgactcg gttaaacagg cactgaagaa ctattttgct caactgaatg
     1261 gtcaggatgt gaatgacctc tatgagctgg tactggctga agtagaacag cccctgttgg
     1321 acatggtgat gcaatacacc cgtggtaacc agacccgtgc tgcgctgatg atgggcatca
     1381 accgtggtac gctgcgtaaa aaattgaaaa aatacggcat gaactaattc aggttagcta
     1441 aatgcttgat taaaaaggcg ctactcggca tggggaagcg ccttttttat aggtgccaca
     1501 aagggagtga ccatgagaac aggatgtgaa ccgacccggt ttggtaatga agctaagacc
     1561 attattcacg gtgatgcgct tgccgaactt aaaaagatcc ccgccgaaag tgtcgatctg
     1621 atctttgccg acccaccata taacatcggt aaaaattttg atggtctgat cgaagcctgg
     1681 aaagaagatc tgtttatcga ctggctgttt gaagtgattg cagagtgcca ccgcgttctg
     1741 aaaaagcagg gcagcatgta catcatgaac agcacggaaa acatgcccta tatcgatctc
     1801 cagtgccgca agctttttac catcaaaagt cgcatcgtct ggtcatatga cagttctgga
     1861 gtacaggcga aaaaacacta cggctccatg tacgaaccca tcctgggtga tgctgccaac
     1921 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttct
//
LOCUS       DAJSKJ010000288         1969 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000288 DAJSKJ010000000
VERSION     DAJSKJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.293"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(68..1480)
                     /gene="pykF"
                     /locus_tag="ON345_003854"
     CDS             complement(68..1480)
                     /gene="pykF"
                     /locus_tag="ON345_003854"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR3073828.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          498 a          453 c          481 g          537 t
ORIGIN      
        1 tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga tatacaaatt aattcacaaa
       61 agcaatatta caggacgtga acagatgcgg tgttagtagt gccgctcggt accagtgcac
      121 cagaaaccat aactacgacg tcacctttgt gtgccagacc gctctgcaga gccagttctt
      181 tacccagacg gtagaaatca tcagtagaag tgatctcttt aaccagctgc ggcacaacgc
      241 ctttgctcag taccaactga tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg
      301 catccgggaa gtatttacgt actgcgcgag cagatttacc gccctgagta gcaaccacga
      361 tcagcggagc atccagtttt tcagcagttt caacggcacc acggcatacc gcttcggtaa
      421 tgcgcagttt acggttgtca ttgttgaact cgagacggct gttcatcacg cggtcggtac
      481 gttcgcagat ggtcgccatg atagaaaccg cttccagcgg gtatttacct tttgcggatt
      541 caccagacag catcactgcg tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt
      601 ctgcgcgagt cgggcgtggg tttttgatca tggaatccag catctgggtc gcagtgataa
      661 cgactttacg tgcacggata catttttcga tcatcatctt ctgggcgaag ataacttctt
      721 ctaccgggat ttctacaccc aggtcgccac gcgcaaccat gatgccgtca gaggcttcga
      781 ggatttcgtc gaagttgttg aggccttcct ggttttcgat tttggagatg atgtggatgt
      841 tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa
      901 taaaggaagc agcaacaaag tctacgcctt gttcgcaacc aaagatcagg tcctgtttgt
      961 ctttttcagc cagtgctggc agagcaatgg aaacgccagg caggttcaca cctttgtttt
     1021 cgcccaggtc accgttgttc agcactttac agataacttt gttaccttca atggcggtaa
     1081 cttccatacc gatcagacca tcgtcaacca gtacggtgtt gccaactgac aggtcagtag
     1141 tgaaaccttc atacgttacc gcaaccattt cgctgttgcc gataacagat ttatcagtgg
     1201 tgaaagtaaa ggtctgacca gctttcagag aaacgtcgtt accgccttcc agtttcatgg
     1261 tgcggatttc cggacctttg gtatcaagca ggatagcggc ggttttacca gttttgctca
     1321 tcacgttgcg cagattctga atgcgctgac cgtgttctgc atagtcacca tgagagaagt
     1381 tcagacgcat aacgttcatg ccagcgtcca gcattttagc taacatctct tcagattcgg
     1441 ttttcggtcc gatggtgcaa acaattttgg tctttttcat gacagtctta gtctttaagt
     1501 tgagaaggat gggagaaact tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta
     1561 cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg
     1621 tgtgcttgtg tctcagccca acgtgagtgc ctgtttatga gtcgttgagt tctacagtcc
     1681 aaagtgctga aaccattcaa gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa
     1741 aggaaaacaa aaacagcgtt tcaaaaaaat acaacttgtt agacaataaa ttgttatcaa
     1801 agatttaacg ttccagggaa aaggttacgc aaaaacttcg cggtttgcgc aacgtctggt
     1861 gccggggttg tcagtgtgag gataagtgaa agttcatccg gtgtataaat atatcatcgc
     1921 tcaattgcta cagttaaaat aatttatcgc gttatttgtc tgattttgt
//
LOCUS       DAJSKJ010000289         1960 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000289 DAJSKJ010000000
VERSION     DAJSKJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.294"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..460
                     /locus_tag="ON345_003855"
     CDS             <1..460
                     /locus_tag="ON345_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpnT protein"
                     /protein_id="HCR3073829.1"
                     /translation="LFSGIAKKKGFPFVILFLSGTDSYGKLDSEVMNASENEIAEMMS
                     LLRGSFVRTLSSELYRHGYACSATLLRRVLAEDSISRSQSKYYTYAASDMKKSIDYSK
                     DIAWTEKIPSTEEYLKSLFIEHKRKYALWEIMLEKIAGLAIEKDSVSYSA"
     gene            complement(507..1781)
                     /locus_tag="ON345_003856"
     CDS             complement(507..1781)
                     /locus_tag="ON345_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR3073830.1"
                     /translation="MSLTDIKAKNAKPLEKEYKLTDGFGMFLRVTPKGSKYWQMAYRF
                     EGKQKLFSIGVYPAVSLSDARQRRDEARRLLAQGIDPNAKKQAEVKELKAKRDNTRTF
                     RTVAKAWFSTKTKWSDDYGDAVWKRLETYAFPVIGDKDVAELDTGDLLVPVKKVEALG
                     YLEVAMRIQQYITAILRHAVQQKLIRHNPAYDMEGAVQKPQTEHRPALELEEIPQLLN
                     KIAEYKGRRLTILAIQLNLMIFIRSSELRFARWSEIDFKSKLWVIPEQREAIENVKHS
                     TRGAKMKRKHFVPLCKQAIRILKEIRQLTYEEGHDDGLIFTGCYDSFKPMSENTINKA
                     LRNMGYNTKQDICGHGFRTLACSALIESGLWSEDAVELQMSHKESNSVRAAYTHKAKH
                     LEQRRLMLQWWADYLDASRNGMVRPFEFATNK"
BASE COUNT          525 a          412 c          442 g          581 t
ORIGIN      
        1 tttgttctct ggcatagcaa agaaaaaagg ctttccgttt gttattcttt tcttgtccgg
       61 gacagatagt tatggaaagc ttgattctga agtcatgaat gcgagtgaaa atgaaatcgc
      121 agagatgatg tctttattac gcggttcttt tgttcgtacc ttatcgagtg agctttacag
      181 gcatggttat gcatgttctg caactctgct ccgtagagtg ctggcagagg acagtatcag
      241 tcgttctcag agtaaatact acacctatgc ggcatctgat atgaaaaaaa gcattgatta
      301 cagtaaagat attgcatgga ctgaaaaaat cccttcaaca gaagagtatt taaaatctct
      361 gtttattgag cacaaaagaa aatacgcact ctgggaaata atgctggaaa aaatcgctgg
      421 tttagcgata gaaaaagatt cagtatcata ttcagcatag actgtaatta agcatgaagt
      481 tgaaaggccg cgaacggcct ttccctttat ttatttgtag caaactcaaa cggccttacc
      541 ataccgtttc tgcttgcatc aaggtagtca gcccaccatt ggagcatcag gcgacgttgc
      601 tcaagatgtt ttgccttgtg ggtataagca gcacggacgc tgttactttc cttatggctc
      661 atttgaagct ccacagcgtc ttctgaccat agcccggact caattaaggc actacaggcc
      721 agagtgcgga aaccgtgtcc acagatgtcc tgcttcgtgt tatatcccat attacgcagg
      781 gctttgttga tggtgttttc actcatgggt ttaaacgagt cataacagcc agtgaagatt
      841 aatccatcat catggccttc ttcataagtc agttgtcgga tctcttttag tatccttata
      901 gcttgcttac agaggggaac gaagtgctta cgcttcattt tggcaccacg agtcgaatgt
      961 ttgacgtttt caattgcttc acgctgttcg ggtatcaccc ataacttact tttgaaatca
     1021 atttcagacc agcgagcgaa acgaagctca ctggaacgaa tgaaaatcat cagattgagc
     1081 tgtattgcca gtatggttaa cctgcggcct ttgtattcgg caattttgtt cagtagctgg
     1141 ggtatttctt ccagctcaag tgcagggcgg tgttcagttt gtggtttctg aactgcacct
     1201 tccatatcat aggctgggtt atggcgtatc agcttttgct ggacggcatg acgcaggatt
     1261 gccgtaatgt attgttgaat gcgcatggca acttcaagat aaccaagagc ctcaactttt
     1321 ttcaccggaa ccagcagatc acccgtatcc agttcggcaa catctttgtc accgattacc
     1381 gggaaggcat aagtttcaag gcgcttccat acggcatcac cataatcatc agaccatttt
     1441 gttttcgtgg agaaccacgc tttggctact gttctgaagg tgcgtgtatt atcacgtttg
     1501 gctttaagct ctttaacctc tgcctgtttc tttgcattag ggtcaatgcc ctgagccaga
     1561 agccttctgg cctcgtcacg gcgttgtctt gcgtcagaaa gagaaactgc agggtaaaca
     1621 ccaatagaga agagtttttg tttcccttcg aagcggtaag ccatttgcca gtatttcgaa
     1681 cctttagggg taacacgaag gaacatacca aagccatcag taagcttgta ttccttctca
     1741 aggggttttg cattttttgc tttaatatca gtcagtgaca tgaaaacacc ccctcatttg
     1801 ttggtaaaag ggaaatcgaa ccagcattac cagcattttt accatcaaaa tgatatggct
     1861 tcgagtggtt tttagtgaac gagggtgaac gactgagagg ggataaccaa ttgatagaaa
     1921 tgcagaaagc agacgttagt gaacgtctgc tttcttgaaa
//
LOCUS       DAJSKJ010000290         1948 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000290 DAJSKJ010000000
VERSION     DAJSKJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.295"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            44..784
                     /locus_tag="ON345_003857"
     CDS             44..784
                     /locus_tag="ON345_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3073831.1"
                     /translation="MSVVIEFSNVTKEYPLYHHIGSGIKDLLFHPKRAMSLLKGRKYL
                     AIEDISFTVNKGESVALIGRNGAGKSTSLGLVAGVLKPSKGEVKVKGRVASMLELGGG
                     FHPELTGRENIILNATLLGLRRSEVYKKLDKIIEFSELGSFIDEPIRVYSSGMLAKLG
                     FSVISQVDPDVLIIDEVLAVGDISFQRKCINTIKAFKERGVTILFVSHSMTDVKKVCD
                     KVIWVENHRLKAIGDADSIIDEYVASMA"
     gene            792..1946
                     /gene="glf"
                     /locus_tag="ON345_003858"
     CDS             792..1946
                     /gene="glf"
                     /locus_tag="ON345_003858"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023284018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HCR3073832.1"
                     /translation="MMQKNILIVGAGFSGAVIGRILAEAGHKISIIDGRSHIAGNCYS
                     ERDNETGVMVHTYGPHIFHTDNAEVWEYISQFTEMMPYTNRVKTTVNGQVFSLPINLH
                     TINQFFKTTCNPEEARKLIEEKSDKSIVEPTTFEEQALRFVGRELYEAFFKGYTIKQW
                     GMSPSELPASILKRLPVRFNYDDNYFNHKYQGMPKDGYTEIVEKILDHSNITTTLNTN
                     FDHASRDEYDHVFFSGALDGFYNYELGRLGYRTLDFDMFRAEGDVQGCAVMNYGEQAV
                     PYTRITEHKYFAPWEQHDKSICYREFSRKCEPNDIPYYPIRQVGEMALLDSYLDKAEK
                     EDNITFIGRLGTYRYLDMDVTIAEALKTARIYLNSLETSTVMPVFTVQVR"
BASE COUNT          591 a          339 c          431 g          587 t
ORIGIN      
        1 gggtgtttaa taaactcaaa ttccgttttg cagagatatt atgatgtcag ttgtcatcga
       61 attttccaac gttacaaaag aatacccact ttatcaccat atcggctctg gtattaaaga
      121 tcttttattt caccctaaga gagcaatgag tctcttaaaa ggacgtaaat acttagctat
      181 tgaagatatt tcttttaccg ttaacaaagg tgagtcggtt gcactgattg gccgtaatgg
      241 cgcaggtaag agtacatcct taggcttggt agctggagtg ttaaaaccta gtaaagggga
      301 agtaaaggtc aaaggtcgtg ttgcatccat gcttgaactg ggaggcggat ttcatcctga
      361 gttaactgga cgcgaaaata taatattaaa tgcgacactc ttaggattaa ggcgctctga
      421 agtctataaa aagctcgata aaattattga gttttctgag ttaggtagtt ttatcgatga
      481 gcctattcgt gtctattcta gcggtatgtt agcaaaatta ggtttttccg taatcagcca
      541 agtagatcca gatgttttaa tcattgatga ggtcttggct gttggtgaca tttcgtttca
      601 aagaaaatgc attaatacga ttaaagcatt taaagaaaga ggtgtgacaa ttttatttgt
      661 cagtcatagc atgactgatg ttaaaaaagt gtgtgataaa gtaatttggg tagagaacca
      721 ccggctgaaa gccattggtg atgcggactc tattatcgat gaatacgtcg cctctatggc
      781 gtgataggca aatgatgcaa aagaatattt taattgttgg tgcgggtttc tctggggcag
      841 ttattggccg tattctggca gaagctggtc ataaaattag tattatcgat ggtcgttccc
      901 atatcgcagg aaactgttat agtgaacgcg ataatgaaac tggtgtcatg gtacatactt
      961 atggcccaca tatattccat accgataatg ctgaagtttg ggaatatatc tcacaattta
     1021 cagaaatgat gccttatact aatcgggtaa aaacgacagt aaatggccaa gtgttttctc
     1081 tgccaatcaa tttgcacaca attaatcagt ttttcaaaac aacttgcaat cctgaagaag
     1141 ctcgcaagct gatcgaagag aaaagtgata agagtattgt tgagccaaca actttcgaag
     1201 agcaagcatt acgctttgta ggtcgtgaat tgtatgaggc tttctttaaa ggttacacta
     1261 ttaagcaatg gggaatgtca ccttcagagc ttccagcatc cattctgaaa cgcttacccg
     1321 tccgctttaa ttatgatgat aactatttca atcataaata ccaagggatg ccaaaggatg
     1381 gatatactga gatcgttgag aaaattctcg accacagtaa tatcacaact acgttgaata
     1441 ctaactttga tcatgcgtcg cgtgacgaat acgatcatgt attctttagc ggcgcactgg
     1501 atggtttcta taattatgag ttaggtcgct taggttatcg tacgttagat tttgatatgt
     1561 tccgcgctga aggcgatgtg caaggatgtg ccgtaatgaa ttacggcgaa caagccgttc
     1621 cttacactcg aatcactgaa cataaatatt tcgcgccttg ggagcaacat gataaatcaa
     1681 tttgctaccg tgaatttagt cgtaagtgtg aaccaaatga tatcccttac tatccgattc
     1741 gccaggtagg cgagatggca ctgctggaca gttacctgga taaagctgaa aaggaagata
     1801 acatcacctt tattggtcgc ttaggtacct atcgctatct agatatggat gtcactattg
     1861 cagaggcatt gaaaacagcg agaatttatc tgaattcact cgagaccagt actgtgatgc
     1921 ctgtgtttac ggtacaagtt agatgaat
//
LOCUS       DAJSKJ010000291         1929 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000291 DAJSKJ010000000
VERSION     DAJSKJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.296"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            91..423
                     /gene="hdeA"
                     /locus_tag="ON345_003859"
     CDS             91..423
                     /gene="hdeA"
                     /locus_tag="ON345_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR3073833.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIAQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            539..865
                     /gene="hdeB"
                     /locus_tag="ON345_003860"
     CDS             539..865
                     /gene="hdeB"
                     /locus_tag="ON345_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR3073834.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            929..1576
                     /locus_tag="ON345_003861"
     CDS             929..1576
                     /locus_tag="ON345_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR3073835.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1618..>1929)
                     /locus_tag="ON345_003862"
     CDS             complement(1618..>1929)
                     /locus_tag="ON345_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3073836.1"
                     /translation="PFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSVPEAAVSLS
                     RKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYL
                     V"
BASE COUNT          562 a          384 c          448 g          535 t
ORIGIN      
        1 caacatgaca tatacagaaa accaggttat aacctcagtg tcgaaattga ttcgtgacgg
       61 ctctttcact ttatagttga ggatattacg atgaaaaaag tattaggcgt tattcttggt
      121 ggtctgcttc ttctgccagt tgtgagcaat gcagcggatg cgcaaaaagc agctgataac
      181 aaaaaaccgg tcaactcctg gacctgtgaa gatttcctgg ctgtggacga atccttccag
      241 ccaactgcag ttggttttgc tgaagcgctg aacaacaaag ataaaccaga agatgcggtt
      301 ttagatgttc agggtattgc aaccgtaacc ccagctatcg ctcaggcttg tactcaggat
      361 aaacaagcca actttaaaga taaagttaaa ggcgaatggg acaaaattaa gaaagatatg
      421 taattccggg aatgcgttac atcgtacttc cttgcatatt gaacaggccg gaatatcttc
      481 tttaaaagca gctattcctc ctgttcatat ataatctcta tattgaatgg gttacaaaat
      541 gaatatttca tctctccgta aagcgtttat ttttatgggc gctgtagcgg ctttgtcact
      601 ggtgaacgca caatctgcgc tggcagccaa tgaatccgct aaagatatga cctgccagga
      661 atttattgat ctgaatccaa aagcaatgac cccggttgca tggtggatgc tgcatgaaga
      721 aacagtatat aaaggtggcg ataccgttac tttaaatgaa accgatctca ctcaaattcc
      781 taaagtgatc gaatactgta agaaaaaccc gcagaaaaat ttgtatacct tcaaaaatca
      841 agcatctaat gacttgccga attaatgagg tacaagtaaa aaggagtagc aagttgagcc
      901 atcttgctgc tcctttttgc atttttatat gacagcagaa tttattatac gtcttatact
      961 cgcggcaatt gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca aaggagcagg
     1021 gttacgcaca catgtattaa ttggcatggg aagcgccctg tttatgattg tttcgaaata
     1081 tggttttgct gacgtgctgt ctttagatca cgtcggactc gaccccagcc gtattgctgc
     1141 tcaggtggtg acgggcgtcg ggtttatcgg tgcaggtaac attctcgttc gtaaccaaaa
     1201 tattgtaggt ctgacgacgg cagcggatat ctgggtgacc gccgccatag gtatggttat
     1261 tggcagcggt atgtacgaac tgggtattta tggttcagtg atgaccttgt tggtgctgga
     1321 agtcttccat caattaacct tccgcctgat gaacaaaaat taccatttac agctgacatt
     1381 agtgaacggt aatacggtat caatgctcga ttggtttaag cagcaaaaaa taaaaacgga
     1441 tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc gccattgata tccagcttca
     1501 tgccacaacg tcgatagaag acctgttgcg attattaaaa ggtatggcag gggtgaaagg
     1561 cgtttcaatt agctaatctg tgtggcagcg tagccagact caccgtaagc ctgaaattca
     1621 caccagataa tcaatatgct gatggcgtac cagctcattg atccgcttaa gattgaggat
     1681 catcatgata ttgtgtttat ggcagtagaa cgtttttagc gatattttca gtttctccag
     1741 aatatcttcc gttgtcattt gattcgcaat gcagctcaga acctgatgtt gcttgcgcga
     1801 aagtgacaca gccgcttcag gcacagagca gtaactgcca ttgatgagtg cgacaaaatc
     1861 aatgatagtg atagtccgcg gaacaaaggt caccggcact ttccccaggc agcgtttaat
     1921 attaaaggg
//
LOCUS       DAJSKJ010000292         1923 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000292 DAJSKJ010000000
VERSION     DAJSKJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.297"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            31..>1923
                     /gene="yjcS"
                     /locus_tag="ON345_003863"
     CDS             31..>1923
                     /gene="yjcS"
                     /locus_tag="ON345_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR3073837.1"
                     /translation="MMVNTANAKEEAKAATQYTQQVNQNYAKSLPFSDRQDFDDAQRG
                     FIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETVNPSLWRQSQLNGISGLFKVTD
                     RMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAALDLYFQNRPQKPIVAVIYTHS
                     HTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISENVLAGNIMSRRALYSYGLLLA
                     HNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMIIDGLEFDFLMTPGSEAPAEMH
                     FYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWTEYLNETLDMWGNDAEVLFMPH
                     TWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYTMNEIGDMIKLPPALANNWASR
                     GYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMGKRYVQALGGSARVINLAQEAN
                     KQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLGYQAESATWRGFYLTGAKELRE
                     GVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKAAGKNISLNFNMSNGDNLNLTL
                     NDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMADLVKAKKAKIIGNGAKLEEII
                     ACLDNFD"
BASE COUNT          502 a          521 c          491 g          409 t
ORIGIN      
        1 attgttattg cgttaactgc cgccagcggc atgatggtaa ataccgctaa cgcgaaagag
       61 gaagcgaaag ccgccactca atatacccaa caggttaatc agaattacgc caaatcatta
      121 ccgtttagcg atcgtcagga ttttgacgac gctcagcgtg gatttatcgc tccgctgctg
      181 gatgaaggta ttctgcgcga tgctaatggc aaaccatatt atcgcgggga agattataaa
      241 tttgatatca atgcgcccgc gcccgaaacc gttaacccta gcctgtggcg tcagtcgcag
      301 ttaaacggta tttccggcct gtttaaagtc accgacagaa tgtaccaggt tcgcggtcag
      361 gatatctcca acatcacctt tatcgaaggt gataccggga ttattgtcat cgacccgctg
      421 gtgacgccac cgagcgcaaa agccgccctt gacctttact tccaaaatcg cccgcaaaaa
      481 ccgattgttg cggttattta tacccacagc cataccgacc actatggcgg cgtgaaaggc
      541 attatctctg aagctgatgt gaaatccggt aaagtgcagg ttatcgcccc tgctggcttt
      601 atggacgaag ccatcagcga aaacgtactg gcgggtaata ttatgagccg ccgtgcactt
      661 tactcctacg gcctgctgct ggcgcataac cctcagggta acatcggcaa tggtcttggc
      721 gtaacgctgg catcgggcta cccgagcatc atcgcaccga acaaaaccat caccaaaacc
      781 ggtgagaaga tgattatcga cggcctggag tttgacttcc tgatgacccc aggtagcgaa
      841 gcaccagccg aaatgcactt ctatattccg gccctgaaag cgctgtgtac cgccgagaac
      901 gccacgcata ccctgcacaa cttctacact ctgcgcggcg cgaaaacccg cgacaccagc
      961 aagtggaccg agtatctgaa cgaaacgctg gatatgtggg gtaacgacgc ggaagtcctg
     1021 tttatgccgc acacctggcc ggtctggggc aataagcata tcaatgatta tattggtaaa
     1081 tatcgcgata ctatcaagta cattcacgac cagaccctgc acctggcgaa ccagggctac
     1141 accatgaatg aaatcggcga catgattaaa ctgccgcctg cacttgccaa taactgggcc
     1201 agccgtggct attacggttc tgtcagccac aacgcccgcg cggtgtataa cttctatctt
     1261 ggctattacg acggtaaccc ggctaacctg catccgtatg gtcaggtgga gatgggtaaa
     1321 cgttacgtgc aggcgctggg cggttccgca cgcgttatta acctggcgca agaagcgaac
     1381 aagcaaggtg attaccgctg gtcggcagaa ctgctgaaac aggtgattgc cgccaacccg
     1441 ggtgaccagg tcgcgaagaa tctgcaagcg aataactttg aacagctggg ctatcaggcc
     1501 gagtccgcca cctggcgcgg tttctacctg accggcgcga aggagctgcg cgaaggggtg
     1561 cataagttca gccacggcac caccggttcc ccggacacca ttcgcgggat gtcggtcgaa
     1621 atgctgttcg actttatgtc cgttcgcctc gatagcgcga aagccgcggg taaaaatatc
     1681 agcctgaact tcaatatgag caatggcgat aacctcaacc tgacgctgaa cgatagcgtg
     1741 cttaactacc gtaaaacgct gcaaccgcaa gctgacgcct ctttctacat cagccgtgaa
     1801 gatctgcacg ccgtgctgac cggacaagcc aaaatggcgg atctggtaaa agcgaagaaa
     1861 gccaaaatta ttggcaatgg cgcgaaactg gaagaaatta tcgcctgtct ggataatttc
     1921 gat
//
LOCUS       DAJSKJ010000293         1864 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000293 DAJSKJ010000000
VERSION     DAJSKJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.298"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            122..376
                     /gene="bssS"
                     /locus_tag="ON345_003864"
     CDS             122..376
                     /gene="bssS"
                     /locus_tag="ON345_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR3073838.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            491..1609
                     /gene="solA"
                     /locus_tag="ON345_003865"
     CDS             491..1609
                     /gene="solA"
                     /locus_tag="ON345_003865"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR3073839.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1657..1770
                     /locus_tag="ON345_003866"
     CDS             1657..1770
                     /locus_tag="ON345_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR3073840.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          479 a          452 c          443 g          490 t
ORIGIN      
        1 ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt
       61 cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat
      121 gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac
      181 cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga
      241 gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg ccagacaatt
      301 tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga
      361 gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt
      421 accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag
      481 agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc
      541 cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc atatgccacc
      601 acatcaacac ggcagccacc atggcgatac gcgattaatt cgccatgctt atggtgaagg
      661 cgaaaagtat gtcccgctgg tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca
      721 caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc cggctgactc
      781 cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct
      841 cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg
      901 cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca
      961 actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcgtca
     1021 tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat
     1081 tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcccgtacg
     1141 taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc
     1201 gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc
     1261 gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc
     1321 gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca atgtattgcc
     1381 gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga
     1441 ttttattatc gataccctac ccggccacga taatacactg ctcattaccg gcctgagtgg
     1501 gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaagacaa
     1561 aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg
     1621 gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc
     1681 atcaataata ttcgcgagca tctgatgctt tatctttttc tctggggatt gttggcaatt
     1741 atggatctga tctatgtgtt ttatttttag ttttccgcac tgacataaac tgacaaatat
     1801 ttaatctccc tttcatttag cgtgaaaaat tgaatgagat tattcctgct tctacagcac
     1861 tcac
//
LOCUS       DAJSKJ010000294         1846 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000294 DAJSKJ010000000
VERSION     DAJSKJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.299"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            54..410
                     /locus_tag="ON345_003867"
     CDS             54..410
                     /locus_tag="ON345_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR3073841.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            414..1541
                     /gene="dapE"
                     /locus_tag="ON345_003868"
     CDS             414..1541
                     /gene="dapE"
                     /locus_tag="ON345_003868"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR3073842.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1569..1769
                     /locus_tag="ON345_003869"
     CDS             1569..1769
                     /locus_tag="ON345_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR3073843.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
BASE COUNT          446 a          434 c          505 g          461 t
ORIGIN      
        1 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
       61 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
      121 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
      181 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
      241 gtaaactgga cgaaaccacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
      301 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
      361 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
      421 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
      481 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
      541 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttggcct
      601 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
      661 ttgaacccac cattcgtgat ggcatgttat tcggacgcgg tgcggcagat atgaaaggct
      721 cgctggcggc gatggtggta gcggcagaac gctttgtcgc acaacatccc aaccatacgg
      781 ggcgactggc atttctgatc acctctgatg aagaagccag tgcccacaac ggtacggtaa
      841 aagtcgtcga agcgttgatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
      901 cgtcgagtat cgaagtggtt ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
      961 gcaaccttac cattcatggc gttcaggggc atgtggccta cccgcatctg gctgacaatc
     1021 cagtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
     1081 atgaattttt cccggcgacc agtatgcaga ttgctaatat tcaggcggga acgggcagta
     1141 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
     1201 ctgatgagat gatcaaagcg caggtgcttg ccctgcttga aaaacatcaa ctgcgctata
     1261 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
     1321 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
     1381 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
     1441 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
     1501 cccgtatgta tcaacgtatc atggaacagc tcgtcgcctg atgagtggtt ctgcaagagg
     1561 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
     1621 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
     1681 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
     1741 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
     1801 gttaacgccg catccggcaa tggtgaacga tgcctgatgc gacgct
//
LOCUS       DAJSKJ010000295         1806 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000295 DAJSKJ010000000
VERSION     DAJSKJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.300"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            21..671
                     /locus_tag="ON345_003870"
     CDS             21..671
                     /locus_tag="ON345_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073844.1"
                     /translation="MLRIGQVESSATVDGKYTDGSVAGGIAATRLRAAAFNAIQEELA
                     NIVESAGMTLDPDDVTQVLAALKKQFLSRVNPFADIKADGAVAVATALSNLGLGTSAK
                     KNVGTASGQIPDMSSFVSAGASNVFSSIVIAGNRRIMEGIGSGTFVNGVLSFTLPLAY
                     PDSGFTFIPTDVGAGVVVYAAQSVSGSVVNIYGKNTGGGVLNGSFGFKYMAMGSAS"
     gene            671..1054
                     /locus_tag="ON345_003871"
     CDS             671..1054
                     /locus_tag="ON345_003871"
                     /inference="COORDINATES: protein motif:HMM:NF014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR3073845.1"
                     /translation="MFYSTEIGFTVGRANDGQVEITEEYWSELVIGQAAGRVISADEK
                     GKPILIDAAPKTNEQLVAEAEQQKQQLISEAQQSISLLQTKLLMGRALTDEEKQKVTT
                     VLDYIDEVEAINTSTIPVNFPPLVI"
     gene            complement(1029..1352)
                     /locus_tag="ON345_003872"
     CDS             complement(1029..1352)
                     /locus_tag="ON345_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR3073846.1"
                     /translation="MHILTLGHNNPRGYKFEDGQLIAPTSEELEQIQKMDTTSAAELK
                     KATLKSAADAEISWRQDAVDAEIATAEEAAALSEWKKYRALMIRIKTDAASYIDWPIS
                     LMVGN"
     gene            1535..1642
                     /locus_tag="ON345_003873"
     CDS             1535..1642
                     /locus_tag="ON345_003873"
                     /inference="COORDINATES: protein motif:HMM:NF014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073847.1"
                     /translation="MAVGKSTIILWQSELLLGGGREKDKTSLVQWMGYI"
BASE COUNT          489 a          365 c          468 g          484 t
ORIGIN      
        1 cttactagtg aggattcgtc atgctcagaa tcgggcaggt tgagtcgtct gcaacagtag
       61 acggaaaata tacagacggt agcgttgctg gtggaatcgc agcgacacgg ctccgggcgg
      121 cggcttttaa tgccatacag gaagaattgg ctaacatcgt agagtcggca ggaatgaccc
      181 ttgatcctga tgatgtgact caggttctcg ccgctttaaa aaaacagttt ctcagccgtg
      241 ttaacccatt tgctgacatt aaagctgatg gtgcagtagc ggtggcgaca gctctctcaa
      301 accttggttt gggaacgtca gcgaaaaaga atgtcggaac agcatcaggt caaatccctg
      361 acatgtctag tttcgtatca gcaggcgcat caaacgtatt ttcttctatc gtcattgccg
      421 ggaatcggag aattatggaa ggtattggtt ccggtacgtt tgtaaatggg gtgttgagct
      481 tcacgctccc tctggcatac ccagactcag ggttcacatt tatacccaca gatgttggtg
      541 ctggcgtggt ggtttacgca gcacagtcgg tttctggttc agtggttaac atatacggca
      601 aaaatacagg tggcggtgtg ctgaatgggt cgtttggttt taaatacatg gcaatgggga
      661 gcgcgtcata atgttctatt caacagagat tggatttacg gttgggcgtg cgaatgacgg
      721 ccaggtagaa attaccgaag aatactggtc ggagctggtt attgggcagg ccgctgggag
      781 agttatttcg gctgatgaaa agggaaaacc catattgatt gatgctgccc caaagacaaa
      841 tgagcagctt gttgcagagg ctgaacagca aaaacagcag cttatttcag aagcacagca
      901 gtcaatttca ttgcttcaaa ccaagctgct tatggggcgt gcgcttactg atgaagagaa
      961 acagaaagtg accactgtac ttgattacat tgatgaagtt gaggcgataa acacctcaac
     1021 tattcccgtt aatttcccac cattagtgat ataggccaat caatatagga ggcagcatca
     1081 gttttgatac gtatcattag cgcccggtat tttttccatt cagacaaagc ggcggcttct
     1141 tccgccgtcg ctatctccgc atcaacagca tcctgcctcc acgatatttc agcatcagca
     1201 gctgatttca aggtcgcttt cttcagctct gccgcagagg tggtatccat tttctggatt
     1261 tgctcaagtt cttctgaggt tggtgctatt aactgaccat cttcgaattt ataaccccgc
     1321 gggttattgt gcccaagagt gagaatgtgt atctcgctga aatgtagcta gagtattgat
     1381 ttttgaaagg tgagattttt attgccataa tgaagttaaa gttccgccac gcggatctcc
     1441 agtaatctca tgcaacatgt actgagggga ttaatgctcc agcctaacaa agactcaact
     1501 agtcaattct gcggagcgaa aaaaagcggc attattggcc gtgggtaaat caacaattat
     1561 cctctggcag tcagagctac tgctgggcgg ggggcgtgaa aaggataaga cgagtcttgt
     1621 tcaatggatg ggttatattt agaaactgaa ggagatggtc gtcgtagatg ttaatgatga
     1681 agctggattt gacgatatac agttgccgga aaagcatgac cagtgaagaa aaaagcccgc
     1741 attgcgggca atttgattat cagattgaaa agggagtgaa ccactttaaa tctgggtcat
     1801 atataa
//
LOCUS       DAJSKJ010000296         1788 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000296 DAJSKJ010000000
VERSION     DAJSKJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.301"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>1788
                     /locus_tag="ON345_003874"
     CDS             <1..>1788
                     /locus_tag="ON345_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HCR3073848.1"
                     /translation="MIRRTIREHLDKELRLNPQQIKVLTLFFIDKVDFYRQTNEEGLL
                     EPGEYARIFEEEYLKAIKSPAYKTLVPSHPEHAKDVHDGYFSIDKKGAWAETTESNNA
                     GRENAERGYNLIMKNKEKLLSFSNPVRFIFSHSALKEGWDNPNVFQICSLREMGSERE
                     RRQTLGRGLRLCVNQEGERVRDDSINILTVIASENYEAYAERLQSEIENETGIRFGII
                     EKDAFAHLIASDYPQGVGIAGSHEIWAELQTEGYLEKSGKITKALKNVLFEDTFVVSE
                     KYASLQEQIETILRKSAGRLNINNADERKTIKYRKEVLNSPEFEALWQRICQKTLYRL
                     AFDEEELIKSCTKSLSEMQPVAKAMVSFSKATIKQSQSGLDVKALSNGTTSTVIDVAE
                     QPLPDILGVLQEKTGLTRRSLSRILKESEKLADFAKNPQQFISEAITRINYCKRLSIV
                     DGIKYYPLKGEVYAQSLFMDKELTGYARNLFETSAKSVYEYVPKDSEVESRFAQELEE
                     QDEVKLYVKLPGWFKIPTPLGTYNPDWALVIEDNGEEHIYFVVETKSKHIGLGHEEDA
                     KIACGTAHFSALKAEEINAAKYVRATAIT"
BASE COUNT          584 a          339 c          401 g          464 t
ORIGIN      
        1 atatgattag acgtacaatc cgtgaacatc tggataaaga gcttcgcctt aatcctcagc
       61 aaatcaaggt tcttacccta tttttcattg ataaggttga tttctatcgc cagactaatg
      121 aagaaggact gcttgaaccg ggtgaatatg cccgcatctt tgaagaagaa tatctaaagg
      181 ccattaaaag ccccgcatat aaaactttgg ttccttctca tccagaacat gctaaagacg
      241 ttcatgatgg ttatttctcc attgataaaa aaggtgcatg ggctgaaaca accgaaagca
      301 ataatgcagg ccgcgaaaat gcggaacgcg gttataacct catcatgaaa aataaagaga
      361 agcttttgag tttctctaat ccggtgagat ttattttttc acactctgct ttgaaagaag
      421 gctgggataa ccccaatgta ttccagatct gtagccttcg agagatggga agtgaacgag
      481 aacgtcgtca aacattaggg cgtggtcttc gcctgtgtgt taaccaagag ggtgaacgtg
      541 ttcgtgatga cagcattaac attctgactg taattgccag tgaaaattat gaagcctatg
      601 ctgaacgact acagagtgaa attgagaacg aaacaggcat ccgttttggt atcatcgaaa
      661 aagatgcgtt tgcacatctg atcgcctctg attacccgca aggtgtaggt atcgcaggtt
      721 cacatgagat ttgggctgaa ctacagaccg aaggatatct ggaaaaatcg gggaaaatca
      781 caaaagccct gaaaaacgtg ttattcgaag acacttttgt tgttagtgag aagtatgcct
      841 cactacagga acaaattgaa actatcctgc gtaaatcagc agggcgactc aatataaaca
      901 atgctgatga gcgtaaaaca atcaaatacc ggaaagaagt cttaaacagc ccggaattcg
      961 aagcgttgtg gcagcgcatt tgtcaaaaaa cgctgtaccg actggcattt gatgaagaag
     1021 aattgattaa atcctgtacg aaatcacttt ctgaaatgca gcctgtagca aaagcaatgg
     1081 tcagtttcag caaagcaaca atcaaacaaa gtcaatctgg ccttgatgtt aaagctctgt
     1141 ctaatgggac aacaagcact gtaattgatg ttgcagagca gccattgcca gatattcttg
     1201 gtgttctgca ggaaaaaact ggactgacaa gacgcagttt gagccgcatc ctgaaagaga
     1261 gtgaaaaact tgccgatttt gctaaaaacc cacagcagtt tattagtgaa gctatcacca
     1321 gaataaacta ctgcaagcgc ttgtctattg ttgatggtat taaatattat ccactaaaag
     1381 gagaggtgta tgcgcaaagc ctgtttatgg ataaagaact gacgggatat gcccgtaatc
     1441 tttttgaaac ttcggctaag tcagtttatg aatacgttcc aaaagattct gaagtcgaaa
     1501 gccgtttcgc tcaggaactc gaagaacagg atgaagttaa gctttatgtg aagctgccgg
     1561 gatggtttaa aatcccgaca ccattaggga cttataatcc tgactgggcc ttagtcattg
     1621 aggataatgg tgaagaacat atctattttg ttgtcgagac taaaagtaaa cacattggtc
     1681 ttggtcatga agaagatgcg aagatagcct gtggaactgc gcattttagt gctttaaaag
     1741 cagaagaaat caatgcagca aaatatgttc gagccacagc cattacag
//
LOCUS       DAJSKJ010000297         1771 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000297 DAJSKJ010000000
VERSION     DAJSKJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.302"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..659
                     /locus_tag="ON345_003875"
     CDS             <1..659
                     /locus_tag="ON345_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR3073849.1"
                     /translation="TGHAVMLQNAKDRCYRKYILVQIPEALSAEKETQKTAVEYCRKN
                     NLKHNVAELTKERLRRAGKKVREDNPEWKGDVGFRVFKLDTSNIRPWEATADTLSQQI
                     DAYVSPILEGRSEEDLLTELMLKRGIDLSVNIETRQFDGLTVSCVDDGKLITCFTRQI
                     PASSVEELTKGIIDWHKSLKAGKDTVCYFLDDAFENNVAKTNLCAILEQHGLTNLHSL
                     "
     gene            669..>1771
                     /locus_tag="ON345_003876"
     CDS             669..>1771
                     /locus_tag="ON345_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3073850.1"
                     /translation="MKLHFESDLTYQQNAITSVCDLFEGQEKWESEMTVLAPAQGVLS
                     FEGSTGSMPVNPQIPEDDVLLKNLNNVQLKNQLPPSPVLDSHDFTVEMETGTGKTYVY
                     LRTMLELNKRYGMSKFIIVVPSVAIKEGVYKTLQITEEHFKSLYAGTPYEYYLYDSAK
                     PADVRNFATSSVMQIMVMTVGAINKKDVNKLYQSSEKTTVDDLDKPIDLIRATRPIII
                     VDEPQSVDGGLNGAGKTALDAMHPMFTLRYSATHVHKHHMVYRLDAIDAYNQKLVKQI
                     EVAGLEVQNASNSPYVKLVSINSKKNSISATVIVDIADKKGNVTRSEITVHDGMTLDD
                     ETGRDIYQGYRISEIRTGKEGYLTLDTPSNTYY"
BASE COUNT          567 a          360 c          383 g          461 t
ORIGIN      
        1 caactggtca tgcagtcatg ttacaaaatg cgaaagatcg ctgctaccgt aaatatattt
       61 tagtacaaat tccagaagca ctgtcagccg aaaaagaaac tcaaaaaacg gcagtagaat
      121 attgtagaaa aaataattta aaacataatg ttgccgaact aacaaaagaa cgcctgcgtc
      181 gtgcaggtaa aaaagttcga gaagataatc cagaatggaa aggtgacgtt ggttttcgtg
      241 ttttcaaact cgatacatct aatatccgtc cgtgggaagc tactgctgat accctttcac
      301 aacagattga cgcttatgta agcccaatcc ttgaaggccg tagtgaagaa gatttgctca
      361 cagagttaat gctaaagcgt ggcattgacc tgagtgttaa cattgaaacc cgtcagtttg
      421 atggattaac cgtttcttgt gttgacgatg gaaaattgat tacctgcttt acccgtcaaa
      481 tccctgcctc ttctgttgag gaactaacca aggggatcat tgactggcat aaaagtttga
      541 aagctggcaa agatactgtc tgttatttcc ttgatgacgc atttgaaaat aacgttgcta
      601 aaacaaatct ttgcgccatt ctggaacaac acggcctgac taatctgcac agcctgtaag
      661 ggaatgagat gaaactacat tttgaatctg accttaccta tcagcaaaac gccataacat
      721 cagtttgcga tctgtttgaa ggtcaggaaa aatgggaaag cgaaatgact gtgctggcac
      781 cagctcaggg cgttctctca tttgaaggtt caacgggttc aatgcctgtt aacccacaaa
      841 tacccgaaga tgatgttctg ctgaaaaacc tgaacaatgt tcagttaaaa aaccagcttc
      901 caccttcacc tgtgctcgac agccatgatt ttactgtcga aatggaaaca gggacaggta
      961 agacgtatgt ttatcttcgc accatgcttg agttgaataa acgatatggc atgagcaaat
     1021 ttatcatcgt agttccttcc gttgccatca aggaaggcgt ttataaaacg ctacaaatca
     1081 ctgaagaaca ttttaaaagc ctttatgcgg gaactccgta cgaatactac ctgtatgatt
     1141 cagcaaaacc tgcggatgtg cgaaattttg caaccagctc tgttatgcag atcatggtga
     1201 tgacggtggg tgcgattaac aaaaaagacg ttaataagct ctatcagtcc agtgaaaaaa
     1261 caaccgttga cgatctggat aagccaattg atctgatacg tgctacacgc ccaattatta
     1321 ttgtagacga accgcaaagt gtggatggtg gcttaaacgg tgcgggtaaa accgcacttg
     1381 atgctatgca ccccatgttc accttacgct attcagcgac acatgtgcat aaacatcata
     1441 tggtgtatcg cctcgatgcc attgatgctt acaatcagaa actggtgaaa caaatcgaag
     1501 ttgccgggct tgaagtccaa aatgcaagca atagtcccta tgtaaaactg gtcagtatta
     1561 acagtaagaa gaatagcatt agcgcgactg ttattgttga tatagccgat aaaaaaggga
     1621 atgtaactcg ttctgaaatt accgtgcatg acggtatgac acttgatgac gaaacggggc
     1681 gtgatattta tcagggatac aggataagtg agatccgcac gggcaaagaa ggctatctga
     1741 cactggatac gccttctaat acctactata t
//
LOCUS       DAJSKJ010000298         1713 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000298 DAJSKJ010000000
VERSION     DAJSKJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.303"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..532
                     /locus_tag="ON345_003877"
     CDS             <1..532
                     /locus_tag="ON345_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR3073851.1"
                     /translation="FAIFIVAAITCISQPKKTTLRYKAMVNYAFDYLRSPGSLPLTTA
                     ATELSSIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIY
                     LKGLGVKPDTHKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAK
                     QHYKNAERLLAGLPAH"
     gene            complement(542..>1713)
                     /locus_tag="ON345_003878"
     CDS             complement(542..>1713)
                     /locus_tag="ON345_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR3073852.1"
                     /translation="RFLPSAWQRFIQQLNYCSMEEIDELRWSLCTIAMNTAHLSFECV
                     VLLAERLRWLQEENVGEIDEEELESFLYAIAKGNVFNFQTILHLPVAVQNDTIDFYQM
                     FARIWSSHPEWLTLYLAQHRAVIIPDDAKLHRNLLRWYSASRLGIPELLDYARSWREA
                     EPDNEDARYYEYAQRVYCGEGESLLAELCYYWREYPSTQADALMLQWCRQHRVDYYPL
                     VVMMIEARDLVNDQGKPLLYVPGDSARTRFHLYEILSDEKLSALGRSLVEMVLHKGRK
                     PRISLTRDTEHPLWPLYLVAKQLVQASQPTEESLMPIVSRLDAEDRCPLEALIIRRLL
                     IQAANFTGQETVEPEPQPQPMPVDDGGLGCLGVIKIIFYIFIFAGLIGKILHLFG"
BASE COUNT          478 a          418 c          389 g          428 t
ORIGIN      
        1 attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga aaacgacgtt
       61 gcgttataaa gccatggtga attatgcctt tgattattta agatcaccag gcagtctacc
      121 gttaaccacg gcagccacag agctttcctc gattcatggt cactcaacat cgcaatatcg
      181 ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag
      241 atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactggggtg
      301 gatctacctg aaaggcctgg gagtcaaacc cgatacccat aaagcaattc tctggtataa
      361 ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa
      421 tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa
      481 acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact
      541 gtcacccgaa gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa
      601 taattttgat gacgcccaga cagcccagcc cgccatcgtc aacgggcatt ggctgcggtt
      661 gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac
      721 gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca
      781 ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact aaatataatg
      841 gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt cctttgtgca
      901 aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg agtatttcgt
      961 ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttccctgat
     1021 cgttaaccag atcacgcgct tcaatcatca tcaccactaa tgggtaataa tcgacccgat
     1081 gctgacggca ccattgcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt
     1141 agtaacaaag ttctgccagc aggctttcgc cttctccaca atagacgcgt tgcgcgtatt
     1201 cataataacg cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca
     1261 ggagttcggg gattcccagg cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt
     1321 ttgcatcatc ggggatgatc actgcgcgat gttgcgctaa atacaatgtc agccattctg
     1381 gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg
     1441 caacgggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata
     1501 aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca
     1561 atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg
     1621 tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt aattgctgaa
     1681 tgaatcgctg ccaggcggag ggcagaaaac gtt
//
LOCUS       DAJSKJ010000299         1709 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000299 DAJSKJ010000000
VERSION     DAJSKJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.304"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>1709
                     /locus_tag="ON345_003879"
     CDS             <1..>1709
                     /locus_tag="ON345_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR3073853.1"
                     /translation="SAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIAEGQYSSAIGS
                     KTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALGKLSIAMGDSS
                     KAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEENAIALGRSSVA
                     SGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKV
                     NTIALGNGSQALADNAIAIGQGNKADGMDAIALGNGSQSRGLNTIALGTASNATGDKS
                     LALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAING
                     SQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQ
                     TKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNT
                     SNIATNTAKIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKD
                     ADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDA
                     LKW"
BASE COUNT          495 a          409 c          433 g          372 t
ORIGIN      
        1 gatcagctgg cgacggctgg gttgctatag gcaaaggggc gaaagcaaat acttttatga
       61 acaccagtgg ttccagtact gctgtgggtt atgacgctat agctgaaggc caatatagct
      121 ctgccatcgg gtcaaaaacc catgcgattg gtggtgcatc aatggccttt ggggttagtg
      181 caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca ttgggccaat
      241 actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt gctatggggg
      301 actcttccaa agcggaagga gcaaacgcca ttgccctggg aaatgccact aaagctactg
      361 agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat tcaatggcgc
      421 tgggagcaag tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt agcagtgtag
      481 ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca gcgaacgcta
      541 ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc aataatgcga
      601 aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat aaagtcaata
      661 ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc ataggccagg
      721 gcaacaaagc tgatggcatg gatgccatcg ctctgggtaa tggtagccag tcgagaggct
      781 taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt cttgcgcttg
      841 gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat tccattgcgg
      901 atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc gttaatgtga
      961 aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag ctttatgcca
     1021 ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt gatgacggta
     1081 ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta ggggctgcgc
     1141 tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa tacagcgctg
     1201 ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat ggcacgattt
     1261 cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat gacgatgtcg
     1321 aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat acggcaaaga
     1381 ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac cttcaggctg
     1441 atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc caggatacca
     1501 ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact gatgctgtta
     1561 acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc aatatcgcca
     1621 ataacacttc caatattgcc actaacacca ccaacatctc taacctgact gagacggtga
     1681 ctaatcttgg tgaggatgcg ctgaaatgg
//
LOCUS       DAJSKJ010000300         1698 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000300 DAJSKJ010000000
VERSION     DAJSKJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.305"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..96
                     /locus_tag="ON345_003880"
     CDS             <1..96
                     /locus_tag="ON345_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073854.1"
                     /translation="YEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            302..1585
                     /gene="gltA"
                     /locus_tag="ON345_003881"
     CDS             302..1585
                     /gene="gltA"
                     /locus_tag="ON345_003881"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR3073855.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          443 a          411 c          396 g          448 t
ORIGIN      
        1 tatgaagtcg gtatttcacc taagattaac ttatgtaaca gtgtggaagt attgaccaat
       61 tcattcggga cagttattag tggtagacaa gtttaataat tcggattgct aagtacttga
      121 ttcgccattt attcgtcatc aatggatcct ttacctgcaa gcgcccagag ctctgtaccc
      181 aggttttccc ctctttcaca gagcggcgag ccaaataaca aacgggtaaa gccaggttga
      241 tgtgcgaagg caaatttaag ttccggcagt cttacgtaat aaggcgctaa ggagacctta
      301 aatggctgat acaaaagcaa aactcaccct caacggggac acagctgttg aactggatgt
      361 gctgaaaggc acgctgggtc aagatgttat tgatatccgt actctcggtt caaaaggtgt
      421 gttcaccttt gacccaggct tcacttcaac cgcatcctgc gaatctaaaa ttacttttat
      481 tgatggtgat gaaggtattt tgctgcaccg cggtttcccg atcgatcagc tggcgaccga
      541 ttctaactac ctggaagttt gttacatcct gctgaatggt gaaaaaccga ctcaggaaca
      601 gtatgacgaa tttaaaacta cggtgacccg tcataccatg atccacgagc agattacccg
      661 tctgttccac gctttccgtc gcgactcaca tccaatggca gtcatgtgtg gtattaccgg
      721 cgcgctggcg gcgttctatc acgactcgct ggatgttaac aatcctcgtc atcgtgaaat
      781 tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc
      841 cattggtcag ccatttgttt atccgcgcaa cgatctctcc tatgccggta acttcctgaa
      901 tatgatgttc tccacgccgt gcgaaccgta tgaagttaat ccgattctgg aacgtgctat
      961 ggaccgtatt ctgatcctgc acgctgacca tgaacagaac gcctctacct ccaccgtgcg
     1021 taccgctggc tcttcgggtg cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact
     1081 gtggggacct gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg aagaaattag
     1141 ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg attctttccg
     1201 cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg
     1261 tgaaacctgc catgaagttc tgaaagagct gggcaccaaa gatgacctgc tggaagtggc
     1321 tatggagctg gaaaacatcg cgctgaacga cccgtacttt atcgagaaga aactgtaccc
     1381 gaacgtcgat ttctactctg gtatcatcct gaaagcgatg ggtattccgt cttccatgtt
     1441 caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc gcccactgga gcgaaatgca
     1501 cagtgacggt atgaagattg cccgtccgcg tcagctgtat acaggatatg aaaaacgcga
     1561 ctttaaaagc gatatcaagc gttaatggtt gattgctaag ttgtaaatat tttaacccgc
     1621 cgttcaaatg gcgggttaat ttttatatgc ctaaacacaa aaaattgtaa aaataaaatc
     1681 cactatcgga cattcata
//
LOCUS       DAJSKJ010000301         1695 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000301 DAJSKJ010000000
VERSION     DAJSKJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.306"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(47..1444)
                     /gene="ccp"
                     /locus_tag="ON345_003882"
     CDS             complement(47..1444)
                     /gene="ccp"
                     /locus_tag="ON345_003882"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR3073856.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          453 a          447 c          349 g          446 t
ORIGIN      
        1 cgtaataaaa atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct
       61 gcatatacgg cgtgtacacc ccgttcagac tatgcaggaa agctacgata tcatccacat
      121 cctcctgcgg cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca
      181 gcgtcggcac gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg
      241 gtactttctg acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg
      301 ccgccgtaat ttccccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc
      361 caccgagaat aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc
      421 cttttttctg ttgcgccgtc agcgcatttt catctccacg caaccattta tcaaatgggg
      481 aatccggcgt aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac
      541 tgaaaccttg cggatagacg ccgaggaact gcgctttaag ctgcggatct ttttccagct
      601 tagcaataat ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac
      661 cagcctgatc ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg
      721 agttaaatac cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt
      781 ttctgccatc gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat
      841 cagccgataa acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg
      901 taggcagttt ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat
      961 tgctggcgta atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt
     1021 catcactcac cttacccgcc cagtgtagcg cggtataacg cgttggtggc atagtttcat
     1081 actgcatcac ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca
     1141 gtgccgcacg cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca
     1201 tcaactgttt cgcgccagga atataataat aggcgggtaa ttctgccgaa ggcgtgtggc
     1261 aatagtcgca tcctttttcg cggagaaagc ctaaaacctt attattttca ctgacagcag
     1321 atgcctgaac atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata
     1381 accctaaata gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt
     1441 tcattattct tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg
     1501 caaacgtctc tgcgagacaa agccatcata aaaaacagtg agatacgctt tttgatagga
     1561 atcaatcaat tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc
     1621 aggaatggca tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca
     1681 acaccaaatc cgtat
//
LOCUS       DAJSKJ010000302         1676 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000302 DAJSKJ010000000
VERSION     DAJSKJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.307"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..1175)
                     /locus_tag="ON345_003883"
     CDS             complement(<1..1175)
                     /locus_tag="ON345_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR3073857.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGMDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGSLASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHR"
     gene            complement(1371..1676)
                     /locus_tag="ON345_003884"
                     /pseudo
     CDS             complement(1371..1676)
                     /locus_tag="ON345_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          458 a          359 c          364 g          495 t
ORIGIN      
        1 attcgatgta tcacagattc atgttcaggc atgtcggaag cattgcttgt ctccaccgct
       61 agggatgcaa aggcgctggc ataacacagt gctgattcca gtgattttcc tttcgcaagt
      121 cctgatgcca gagagccgtt aaacgcatcc ccggctcctg ccgtattttt aacgaccgct
      181 ggaaacgctg gcgaatgaat gaattttttg ccatcaaatg cgagcgagcc cttgctaccg
      241 agcgtgataa ccgtgttttt taccccttta ttgtggatag cttctgcagc tcgtttagct
      301 gattcaagat cgtgtacgtc catgccggac agtaaaccag cttctgtttc attgggggtg
      361 atataatcaa catcctgaat aagttcgttg acgatatcat tatagggtgc cgggttaata
      421 atgaccggga tactgttttt ttgcgccaat gtgatcgctt gttgcagagc ggtataattt
      481 gtttcgagct gcagaagtat gacatcagaa ttaataatcg cttctttctg aatagttatt
      541 tcatcggacg acattgtcat gttggcaccg ggatagatcg caataacatt atcgccagta
      601 ccttcattaa caaggatcgt ggcagttccg gtttgcgttt ctttcgtttg ataaattata
      661 cttttataaa tcttagacga attcataaag ttgatcgcat aatcactaaa gtgatctgta
      721 cctacttttg ttatgaaata aacatcagta tcggcaaagc ttgctgccag cgcctggtta
      781 cagccttttc cccccggtga aaatataaaa ttacttgcca gaagcgattc tccgatagtg
      841 ggaagcctcg gcaaataact gatgatatca acattaaacg aaccaataac gcatacttga
      901 cttttcatag ttgagtggcc tttttttatt ccctgggctg tatcataatt ttcaagcgca
      961 taagcgattt catttttcgc aatagtatca agcgagtcta ggacgataaa agcaccgcca
     1021 tgacatcgag tcagatagcc aagcctggca agttcattaa gatctgaacg aatagtttct
     1081 ttcgttacat tgaggctatt ggacagatcg gatactttta cagaaccgaa tttactgagt
     1141 cgtttcaata tttcatgatg gcgttcaaat ttcatgcttc acctcacttc agttacgtta
     1201 atatctatcg atgtatattg atgtgataat caccataaag tggcggggaa aagcaaaaaa
     1261 aagcaaaaat agcctgagaa aaggtaagcg ttaatggaga atcatattga tgcttattta
     1321 ttggtgagaa ctacgttcca tggcaggagt tcatcaatac ggttggaagg ttattccggc
     1381 agtacgctca ggatatggcg cagatacgct tccggatcaa tgtcgttcag aaggcaagtg
     1441 ccgataagcc catacaacag tgcactgcgc tcgccgccga agaacataaa gtttttattt
     1501 ccgagacaga tggcacaaag ggctttttcc gcggcattat gtctgcctcc gcctgaccgt
     1561 caacactgta ataacagagt gcgccccact gattcagtac acagctgaac cattctccca
     1621 gtctggattt ttggatagcg tggcattttt ctccaccatg catgcatgca gtgagt
//
LOCUS       DAJSKJ010000303         1668 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000303 DAJSKJ010000000
VERSION     DAJSKJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.308"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(72..515)
                     /locus_tag="ON345_003885"
     CDS             complement(72..515)
                     /locus_tag="ON345_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3073858.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            672..1601
                     /gene="metA"
                     /locus_tag="ON345_003886"
     CDS             672..1601
                     /gene="metA"
                     /locus_tag="ON345_003886"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR3073859.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRQMNPTLD"
BASE COUNT          403 a          462 c          377 g          426 t
ORIGIN      
        1 gttggtgaaa aaaatgccgg gtgcgacgct ggcgcgtctt ctccagcctg ggcaggcacg
       61 gcaggggcgg cttattcctc cacatacgcc agattcagca acggatacgg tttccccaaa
      121 tcgtccacct cagagcgtcc cgtaacctta aaacccacct tcttatagaa cccaaccgcc
      181 tgctcatttt gctcattaac gttggttgtc agttccggtg ccatcgagag cgcatgctcc
      241 accagcatcc gacccacgcc gcagccgcac acatcaggat cgataaacag cgcatccata
      301 tgctgcccac ttagcaacat aaatccaacc ggctgatccc gttcattaac cgcgacccac
      361 aacggtgctt ccggcagaaa ggaacgaacc agctcttcca gctcggcccg atactctgct
      421 gataaaaaat cgtgtgtggc atcgacagaa cgacaccaaa tcgcaacgag ttcctcccct
      481 tcctcatgcc gtgagcggcg aatactaata accattttct ctccttttag tcattcttat
      541 attctaacgt agtcttttcc ttgaaacttt ctcaccttca acatgcaggc tcgacattgg
      601 caaattttct ggttatcttc agctatctgg atgtctaaac gtataagcgt atgtagtgag
      661 gtaatcaggt tatgccgatt cgtgtgccgg acgagctacc cgccgtcaat ttcttgcgtg
      721 aagaaaacgt ctttgtgatg acaacttctc gtgcgtctgg tcaggaaatt cgtccactta
      781 aggttctgat ccttaacctg atgccgaaga agattgaaac tgaaaatcag tttctgcgcc
      841 tgctttcaaa ctcacctttg caggtcgata ttcagctgtt gcgcatcgat tcccgtgaat
      901 cgcgcaacac gcccgcagag catctgaaca acttctactg taactttgaa gatattcagg
      961 aacagaactt tgacggtctg attgtaaccg gtgcgccgct gggcctggtg gagtttaatg
     1021 atgtcgctta ctggccgcag atcaaacagg tgctggagtg gtcgaaagat cacgtcacct
     1081 cgacgctgtt tgtctgctgg gcggtacagg ccgcgctcaa tatcctctac ggcattccta
     1141 agcaaactcg caccgacaaa ctctctggcg tttacgagca tcatattctc catcctcatg
     1201 cgcttctgac gcgtggcttt gatgattcat tcctggcacc acattcgcgc tatgctgact
     1261 ttccggcagc gttgattcgt gattataccg atctggaaat tctggcagag acggaagaag
     1321 gggatgcata tctgtttgcc agcaaagata agcgcattgc ctttgtgacg ggccatcccg
     1381 aatatgatgc gcaaacgctg gcgcaggaat atttccgcga tgtggaagcc ggactagacc
     1441 cggatgtacc gtataactat ttcccgcaca acgatccgca aaacaaaccg cgagcgagct
     1501 ggcgtagtca cggtaatttg ctgtttacca actggctcaa ctattacgtc tatcagatca
     1561 cgccatacga tctacggcaa atgaatccaa cgctggatta atcttctgtg atagtcgatc
     1621 gttaagcgat tcagcacctt acctcaggca ccttcgggtg cctttttt
//
LOCUS       DAJSKJ010000304         1659 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000304 DAJSKJ010000000
VERSION     DAJSKJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.309"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            100..315
                     /gene="essD"
                     /locus_tag="ON345_003887"
     CDS             100..315
                     /gene="essD"
                     /locus_tag="ON345_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR3073860.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            320..631
                     /locus_tag="ON345_003888"
     CDS             320..631
                     /locus_tag="ON345_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR3073861.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            628..1161
                     /locus_tag="ON345_003889"
     CDS             628..1161
                     /locus_tag="ON345_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR3073862.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1158..1655
                     /locus_tag="ON345_003890"
     CDS             1158..1655
                     /locus_tag="ON345_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HCR3073863.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          473 a          343 c          436 g          407 t
ORIGIN      
        1 tttagcgccg ttgagaacaa cgtgtgctgt caaaactacc ccgtagactc cgatcttttt
       61 caaacatatt gcaccatccg tgtacatcgg ggtgaggata tgaaatcaat ggataagtta
      121 acaacaggtg ttgcctatgg cacatcggcg ggtaatgctg gtttctgggc attacagtta
      181 ctcgataaag taactccgtc acagtgggct gcaatcggtg tgctgggtag cctggttttt
      241 ggcctgctga cgtatctgac aaatctttat ttcaagatta aagaagacag gcgtaaggct
      301 gcgagaggag agtaatccaa tgactcaaga ctatgaactg gttgtgaaag gagtccgtaa
      361 ttttgagaat aaagttacgg taactgtagc cttacaggac aaggaacgct ttgacggtga
      421 aatttttgac ctggatgtcg ccatggaccg tgttgaagga gctgcgctgg agttttatga
      481 ggcagcagcc agaaggagcg tccggcaagt cttcctggaa gtagcagaaa aattgtcaga
      541 aaaagttgag tcttatctgc agcatcagta ctcctttaag attgaaaatc ctgccaataa
      601 gcacgagcgt cctcatcata aatatatatg aacacaaaaa tcagatacgg cctgtcggct
      661 gccgttctgg cgctgattgg tgctggcgca tctgctcctc agatacttga ccagtttctg
      721 gacgaaaaag aaggtaacca cacaatggca taccgcgatg gttctggcat atggaccatc
      781 tgtcggggtg ccacagtggt ggatggaaaa accgtttttc ccaatatgaa actgtcgaag
      841 gaaaaatgcg accaggtcaa cgccattgag cgtgataagg cgctggcatg ggtggagcgc
      901 aatattaaag taccactgac cgaaccacaa aaagcgggta tcgcgtcatt ttgtccctat
      961 aacattggcc ccggtaagtg tttcccgtcg acgttttata agcggctgaa tgctggtgat
     1021 cgtaaaggtg catgcgaagc gattcgctgg tggattaagg atggcggacg cgattgccgc
     1081 attcgttcaa ataactgtta cggtcaggtt attcgtcgtg accaggagag cgcattaacc
     1141 tgctggggga tagaacagtg aatcagatat tcatggtgat ttttctcgtg ttgtcaggat
     1201 ttatcgtcgg aaatgtctgg agcgacagag gatggcaaaa aaaatgggcg gaacgtgatg
     1261 ctgccgcatt atcacaagag gtaaatgctc aatttgctgc tcgaataatt gaacaggggc
     1321 gaactatagc ccgtgatgag gctgttaaag atgcacaaca gaaatctgct gaaatttctg
     1381 ccagggctgc ttatctgtct gatagtgtta accagttgcg tgccgaagca aaaaaatatg
     1441 ccatacgcct tgacgcagcg aagcataccg cagatcttgc cgctgccgtc agaggcaaaa
     1501 caaccaaaac cgccgaagga atgctcacca acatgctcgg agatattgca gcagaagctc
     1561 agctttatgc tgaaattgct gacgaacgct acatcgcagg agtgacttgt caacagatct
     1621 atgaatcttt aagagataaa aagcatcaaa tgtagggta
//
LOCUS       DAJSKJ010000305         1615 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000305 DAJSKJ010000000
VERSION     DAJSKJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.310"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(121..756)
                     /gene="msrQ"
                     /locus_tag="ON345_003891"
     CDS             complement(121..756)
                     /gene="msrQ"
                     /locus_tag="ON345_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR3073864.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(757..>1615)
                     /gene="msrP"
                     /locus_tag="ON345_003892"
     CDS             complement(757..>1615)
                     /gene="msrP"
                     /locus_tag="ON345_003892"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR3073865.1"
                     /translation="FKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEF
                     GLDKADPAANAGSLKTDPWRLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWS
                     MVVPWIGFPLHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLR
                     LDEAMHPLTLMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTW
                     NLAAPDEYGFYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLY
                     RGLDLRENF"
BASE COUNT          412 a          411 c          416 g          376 t
ORIGIN      
        1 atactgataa tttgaggttg atgacaaacg aaacataatc tgatgtgatc tgcgtaggcc
       61 ggatagggcg ctcgcgccgc atccggcagg gataacgcgt actttgtcgg caatatgaag
      121 ttatacagat aatttgttgt gaacctgctt gcgtaaccgg ttaaaaaggg aacgcaactt
      181 cttataccgt aaggctaaaa gcagtacagc cagcccagcg tagatgagcg gctgcggtga
      241 aataatcttc actgaccaca gataatgtat tggggccagg atcgcgacaa gatagacgaa
      301 attatgcaac tgttgccagt gcttgccgag ttttcgctgc atcgcctggg ttgaagtaaa
      361 cgctaaagca agcaaaataa cccagctgat aattcctaac gttaaataag gccgggtaat
      421 taactccttg cctaataacg ccagattatt cacgcccagc tccagcaatg cgtaactggt
      481 taaatgcagt gtcgcccagg caaagcacca taagcctaac aggcggcgag tgcgtatcaa
      541 taacggctgt tttgcgtagc gtgccagagg tgtgattaac aaggtcgcca gcaaaaattt
      601 cagtgcagtg cgaccggtaa aatgctgaat atctttcacc ggatcggcac ccagtccacc
      661 gtgattgatc gcccagacca gccagagaaa cggcaacaat ccggcaagat gcaggctaac
      721 tttcagccat gtcacctgtt ttgctgtcag acgcattcag aaattctccc gcaaatccag
      781 gccacgatac agcgatgcca cctggtcggc gtaaccatta aacagtagcg ttggctggcg
      841 ctgaacatcg aggatgccgc ctgaaccaat aaatcgttcg gtagcctgtg accagcgcgg
      901 gtgatcaaca tgcggattaa cgttggcgta aaaaccgtat tcgtcaggcg ctgccagatt
      961 ccaggtggtt ggcggacgct cgcgggtcag cttaatactg actatcgatt taatcccttt
     1021 aaagccatat ttccacggca caatcagtcg taccggcgcg ccattttgtg gcggtaacgc
     1081 cttgccataa acacccacgg tcatcagtgt gagcggatgc attgcttcgt cgagacgcaa
     1141 tccttcgaca taaggatatt tcagcccgcc gccgataaag cggtcctgct ggcctggcat
     1201 ttgttccggt gcataaattg tttcgaaagc gacatacttc gcattgctgg tgggttcggc
     1261 aagcgccagc aatttgtgca gcggaaaacc aatccacggc accaccatcg accatgcctc
     1321 cacgcagcgc atacgataaa tacgctcttc cagcgggaag cgacgggtta aatcatcgtg
     1381 atcgagggtc aatggttttg ccacttcgcc gctgattttc agtctccatg gatcggtttt
     1441 caggctacca gcattagcgg cgggatcggc tttatccagc ccgaattcat agaagttgtt
     1501 ataaccggag actttatcta ctggcgtcag tggcaggtta ttttgccagg cggcaggctt
     1561 gctgaactcc agcggttttc cggcgggggc gggcgggcga tcgttccctt taaac
//
LOCUS       DAJSKJ010000306         1605 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000306 DAJSKJ010000000
VERSION     DAJSKJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.311"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(217..870)
                     /gene="ribB"
                     /locus_tag="ON345_003893"
     CDS             complement(217..870)
                     /gene="ribB"
                     /locus_tag="ON345_003893"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR3073866.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(974..1122)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1244..1534
                     /gene="ubiK"
                     /locus_tag="ON345_003894"
     CDS             1244..1534
                     /gene="ubiK"
                     /locus_tag="ON345_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR3073867.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          457 a          405 c          355 g          388 t
ORIGIN      
        1 aatttcacat atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata
       61 tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag
      121 aagcggaatt aacatcttgc atagcaggga aaaataaaaa ccgaagcctg ctacaacttc
      181 ggtttcttga ttaaggcagt aactaagcag cggttttcag ctggctttac gctcatgtgc
      241 ctgacggtat gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa
      301 ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac
      361 accagccggt ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc
      421 acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag
      481 atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc
      541 agaaacaccg gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata
      601 ggcgctggtg ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt
      661 gatgcacagg caaacaatac cgctaccgtg gcgaatagtc agcgccatct gctcaacggt
      721 catggtttct gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac
      781 cattacaccg cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt
      841 accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta
      901 ccagaatcag ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca
      961 tgtccgacag aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca
     1021 ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga
     1081 tttaccgccg gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta
     1141 atgattagcg gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg
     1201 cactacaatg gcactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa
     1261 aattgagcaa atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg
     1321 ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct
     1381 tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact
     1441 ggcgctgctt gaacaaagaa tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca
     1501 gccagatcca gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat
     1561 tatgaattaa tcttctaatt tacagtgctt tctattacct tatcg
//
LOCUS       DAJSKJ010000307         1545 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000307 DAJSKJ010000000
VERSION     DAJSKJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.312"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            176..1405
                     /locus_tag="ON345_003895"
     CDS             176..1405
                     /locus_tag="ON345_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR3073868.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          383 a          344 c          325 g          493 t
ORIGIN      
        1 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
       61 gcaaccacta acccaccaat agaggggtag atagggcgtt aatctcccat acttaacctg
      121 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
      181 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
      241 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
      301 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
      361 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcagtaacac actactatgg
      421 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
      481 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
      541 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaatcctga atctcatttt
      601 tctgatggga cgccatgcaa ccattcgggt aatgggtttt ttggtattcc cattgattgc
      661 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacgac
      721 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
      781 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
      841 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
      901 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
      961 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
     1021 agcatggctg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     1081 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
     1141 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
     1201 gatcaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag
     1261 cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt atctgatccc
     1321 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
     1381 cgtatcggta atgttcttta gctgatatcc attgtgaatg gttcaaaatt gctacaattt
     1441 tgaaccattc cagttttcat aatgttcatt cgttattatt ccgcactatc acttccctca
     1501 tcatatccta gtattatcag ttagttaaac acacaaagag atctg
//
LOCUS       DAJSKJ010000308         1497 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000308 DAJSKJ010000000
VERSION     DAJSKJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.313"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            2..>1497
                     /locus_tag="ON345_003896"
     CDS             2..>1497
                     /locus_tag="ON345_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR3073869.1"
                     /translation="MAIFHLDFKIVKRSEGKSSVAKAAYHARTRITDERTGDTYDYSR
                     RTDLYGHFILAPVNAPQHIVKDSTALWNEVERVERQQNGQTARYFDVAIPTELNNDDK
                     KKLVLEYCQKNFVDKGMIADIAFHDLDSDNPHAHVMLTLKTIGPEGFGKKERSWNDRK
                     MSVLWRESWASMANSYLAAAGSSERIDHRSLQAQHEEALEKAAVALDNEEKALWLAKA
                     AETNRPPMKRIHSAKWRSKAAQEQRAAEQAVRDATKQEAVEVYKTFSELDLEIIVDVR
                     SFTITHLAEPEEIVLPKTSSSSSANETQRPVLVAPPPHTRMRGVKSYRDKSKVSKVVA
                     CIPPSISISDSGTNTILKTSTSQNPNRASRSAPDHERKDKTTPRQDNIFKRFTTLVID
                     FFKEKFVWARSSRVKHDPVSTEHDKRITENFVFDEVLGHHVSRAEYEKQAKFNSDTYK
                     PTPDEIRRFPSRPKHERVNADNNMDFIPTVSTINDKNHTPRLRPPTRRG"
BASE COUNT          483 a          318 c          305 g          391 t
ORIGIN      
        1 tatggctatt tttcatcttg attttaaaat tgtgaaacga agtgagggca aatcttccgt
       61 tgcgaaagct gcttatcatg ctcgtacaag gataacagat gaaagaacag gcgatacgta
      121 tgattatagc cgccgtaccg atttatacgg acatttcata ttagcacccg ttaatgctcc
      181 tcaacatatt gtaaaagact ctacagcatt gtggaatgaa gttgaacggg tcgagcgtca
      241 gcaaaatggt caaacagctc gttatttcga tgttgctatt cccaccgagt taaacaatga
      301 cgacaagaaa aaacttgtcc ttgagtattg tcagaaaaat ttcgttgata aaggcatgat
      361 tgccgatatc gctttccatg atctcgatag cgacaaccca catgctcacg ttatgttgac
      421 gttaaaaacc attggcccgg aagggtttgg taaaaaagaa agaagctgga atgacagaaa
      481 aatgtctgtg ttgtggcgtg aatcatgggc atctatggcg aacagctacc ttgctgctgc
      541 tggttcgtca gaacgtattg atcatcgttc ccttcaggcc cagcatgaag aagcattaga
      601 aaaagcggcg gttgctctgg ataatgaaga aaaagcacta tggcttgcca aagccgctga
      661 aacaaaccgt cctccaatga agcgtatcca cagtgccaaa tggcgtagta aagcagctca
      721 agagcaaaga gccgcagaac aggctgttcg tgatgctacc aagcaagaag ccgttgaagt
      781 ctacaaaaca ttcagtgagc ttgatcttga aatcatcgtt gatgtcagaa gctttacgat
      841 tacacatctt gctgaaccag aagagattgt attaccaaaa acaagctcca gttcttctgc
      901 caatgaaacg caaagacctg tacttgtagc accacctcct catactcgta tgcgaggtgt
      961 taaatcctat cgtgataaat ccaaagtcag taaggttgtt gcttgtattc cgccatcaat
     1021 cagtatttct gattctggta caaatactat tctgaaaaca tccacctctc aaaacccaaa
     1081 cagggcatcc agaagcgccc ctgaccatga aagaaaggat aagactactc cacgtcagga
     1141 taatattttc aaacgtttca caactcttgt catagatttc ttcaaagaaa aatttgtatg
     1201 ggctagatca agcagagtta agcatgatcc tgtcagtaca gaacatgata aacgtattac
     1261 tgaaaacttt gtctttgatg aagtattagg tcatcatgtt tctcgtgctg aatatgagaa
     1321 acaagctaag tttaacagtg atacttacaa accaacacct gatgagattc gacgtttccc
     1381 aagccgtcct aagcacgaac gagtcaatgc agataataac atggatttta taccgactgt
     1441 ttcaacaata aatgataaaa atcatactcc tcgtttaagg ccacctacac gccgtgg
//
LOCUS       DAJSKJ010000309         1496 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000309 DAJSKJ010000000
VERSION     DAJSKJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.314"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..608
                     /gene="envY"
                     /locus_tag="ON345_003897"
     CDS             <1..608
                     /gene="envY"
                     /locus_tag="ON345_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR3073870.1"
                     /translation="VLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPE
                     VFRLAANHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRI
                     IQSDIQHYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKN
                     ITQVAQLCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(654..730)
                     /locus_tag="ON345_003898"
     tRNA            complement(654..730)
                     /locus_tag="ON345_003898"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:Arg,seq:tct)
     gene            973..>1496
                     /gene="fimZ"
                     /locus_tag="ON345_003899"
     CDS             973..>1496
                     /gene="fimZ"
                     /locus_tag="ON345_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR3073871.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRTIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKL"
BASE COUNT          440 a          336 c          315 g          405 t
ORIGIN      
        1 acgtattaat cacccacatc aaccgtaaca tcatcaacga ttatctgttg tttttaaata
       61 agaacttaac ctgtgtaaag ccctggtcgc ggctggcaac cccagttatc gcttgtcata
      121 gccgtacacc ggaagtgttc cggctagccg ccaaccacag caagcagcaa cccagcaaac
      181 cctgcgaagc ggagttgacg cgcgcattac tttttaccgt attgtctaac tttcttgagc
      241 aatcgcggtt tattgcccta ctgatgtata tcttacgcag cagcgtccgc gacagcgtct
      301 gccgcattat tcaaagcgat attcagcatt actggaatct gcgaattgtc gccagttcgc
      361 tatgtttaag ccccagcctg ctcaaaaaga aattaaaaaa cgaaaatacc agctatagcc
      421 agattgtcac agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa
      481 atatcactca ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt
      541 ttaaggcgtt ttacggcctg acaccgttga attatctcgc caaacagcga caaaaagtga
      601 tgtggtgaag ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg
      661 ccctgcagga ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag
      721 ctaagggcgc gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg
      781 ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg
      841 atttatttcg ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc
      901 cgtattttca tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg
      961 agactggtaa ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga
     1021 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
     1081 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
     1141 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
     1201 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagaacgata
     1261 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatgcc
     1321 gttcagatga tcctctccgg atacacgttt tttcccagcg aaacgcttaa ctatataaaa
     1381 agcaataaat gtagtacgaa tagttcaacg atcactgtgc tatctaatcg tgaagtgacc
     1441 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttatt
//
LOCUS       DAJSKJ010000310         1484 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000310 DAJSKJ010000000
VERSION     DAJSKJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.315"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            92..1483
                     /gene="narK"
                     /locus_tag="ON345_003900"
     CDS             92..1483
                     /gene="narK"
                     /locus_tag="ON345_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR3073872.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          275 a          362 c          414 g          433 t
ORIGIN      
        1 ataaagttat cttatcgttt gatttacatc aaattacctt tagctccaga cactaaggtg
       61 gcagacatcg aaacgagtat cagaggtgtc tatgagtcac tcatctgccc ccgaaagggc
      121 tactggagct gtcataacag attggcgacc ggaagatcct gcgttctggc aacaacgcgg
      181 tcaacgtatt gccagccgca acctgtggat ttccgttccc tgtctgctgc tggcgttttg
      241 cgtatggatg ttgttcagcg ctgttgcggt gaacttaccg aaagtcggtt ttaattttac
      301 gaccgatcag ctatttatgt tgactgcgct gccttcggtt tctggcgcgt tattacgtgt
      361 tccatactcc tttatggttc ctatcttcgg tggtcgtcgc tggacggcgt tcagcaccgg
      421 tattctgatt attccttgcg tctggctggg ttttgccgtg caggatacct ccacgcctta
      481 tagcgtcttc atcatcatct ctctgctatg cggctttgct ggcgcgaact tcgcatccag
      541 tatggcaaac atcagcttct tctttccgaa acagaagcag ggtggcgcgc tggggctgaa
      601 tggtggtctg ggaaacatgg gcgtcagcgt catgcagttg gttgctccgc tggtggtatc
      661 actgtcgatt ttcgcagtat ttggtagcca gggcgtcaaa cagccggatg ggactgagct
      721 gtatctggcg aatgcgtcct ggatatgggt gccgttcctt gccatcttca ctattgcggc
      781 gtggtttggc atgaacgatc ttgctacctc gaaagcctcc atcaaggagc agttgccggt
      841 actcaaacgg ggtcatctgt ggattatgag cctgctgtat ctggcaacct tcggctcctt
      901 catcggcttc tccgcgggct ttgcaatgct gtcaaaaacg cagttcccgg atgttcagat
      961 tctgcaatac gctttcttcg ggccgtttat tggtgcgctg gcgcgttctg caggtggtgc
     1021 attatctgac cgtctgggcg gaactcgtgt cacgctggtg aactttattc tgatggcgat
     1081 tttcagcggc ctgctgttcc tgaccttacc gactgacggg cagggcggaa gcttcatggc
     1141 gttcttcgcg gtcttcctgg cgctgttcct gacagctggg ctgggtagtg gttccacttt
     1201 ccagatgatt tcagtgatct tccgtaaact gacaatggat cgcgtgaaag cagaaggggg
     1261 ttctgacgaa cgtgcgatgc gtgaagcggc aaccgacacg gcagcggcgc tgggtttcat
     1321 ctctgcgatt ggcgctattg gtggcttctt tatcccgaaa gcgtttggta gctcgctggc
     1381 attaacgggt tcgccagtcg gcgcaatgaa ggtatttttg attttctata tcgcctgcgt
     1441 agtgattacc tgggcggtat atggtcggca ttctaaaaaa taaa
//
LOCUS       DAJSKJ010000311         1441 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000311 DAJSKJ010000000
VERSION     DAJSKJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.316"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..1373)
                     /locus_tag="ON345_003901"
     CDS             complement(<1..1373)
                     /locus_tag="ON345_003901"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR3073873.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIA"
BASE COUNT          342 a          385 c          382 g          332 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacccaggt agtcaacgtt gaacaccagt
      481 tcctgcggca gcgcttcttc gtcacgccag ataacccagc cgcagcccag cggagccaga
      541 ccgaatttat ggcctgaagc actgatcgat ttcacacgcg gcaggcggaa gtcccagacg
      601 atatccgggg cgacgaacgg tgccaggaag ccaccgctgg cagcgtcgat gtgcatgtcg
      661 atgtcgatac cggtgtcggc ctggaattta tccagcgcat cgtgcagcgg ttgtgggaac
      721 tcatagttac cggtgtaggt cacgccgaaa gtcggcacca cgccgatggt gttttcgtca
      781 caggcttcaa tcatgcgttt cgggtccata aacaactgac cggggcgcat agggatctca
      841 cgcagctcca catcccagta gcgggcgaat ttatgccagc agatttgtac cggaccgcac
      901 accaggtttg gtttatccgt tggtttgcct gcagcttcca tacgcttgcg ccaacgccat
      961 ttcatcgcca tcccgccgag catacaggcc tcggaagaac caatggtgtt ggtgccaacg
     1021 gcctgaccat ttttcggcgc aggcgcatgc cacagatcgg caaccatatt tacgcaacgc
     1081 aggtcgatgg ctgcggattg cggatattct tctttgtcga tccagttttt attgatcgac
     1141 aaatccatca atttatggac gttttcgtcg tcccaggtct ggcagaaagt ggccaggttc
     1201 tgacgagcgt tgccatcaag atataattca tcattgataa tctggaatgc gacatcatcg
     1261 cgcatttcgt gcagcggaaa tcgttttgac tccgcgatag tagaaatggc ctttgcgcca
     1321 aaacgtgaat cgagtagttc tgagcggaaa tccgttaaca gcttctggtc catttcgaac
     1381 tccttaaatt tatttgaagg caataaaaaa gtaggattta tccgcaatgg aagcaaggca
     1441 t
//
LOCUS       DAJSKJ010000312         1409 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000312 DAJSKJ010000000
VERSION     DAJSKJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.317"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(50..790)
                     /gene="cysQ"
                     /locus_tag="ON345_003902"
     CDS             complement(50..790)
                     /gene="cysQ"
                     /locus_tag="ON345_003902"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR3073874.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            980..>1409
                     /locus_tag="ON345_003903"
     CDS             980..>1409
                     /locus_tag="ON345_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3073875.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVI"
BASE COUNT          343 a          362 c          344 g          360 t
ORIGIN      
        1 gaaatcagga tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatag
       61 acactctgaa ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct
      121 gccagtcgtg aacgtgcgct ccggcagctg cagccacagc atgtccagcg gcggtgtccc
      181 aaatattcgt tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga
      241 atttcagcga agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt
      301 tcagctccgc atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga
      361 cctgaatctg cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt
      421 acattacgtt cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga
      481 gcgcaatgtt aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg
      541 ggtctaccag ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt
      601 cagaaaggac cggaatatcc ggtgtcagcg tacgtaaacc gtccatgata acggtgtgtg
      661 cggcaatatc cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt
      721 tcgtcccgtc gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt
      781 gatctaacat ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac
      841 cctatcgtta atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt
      901 ggcattcttc actgttctat aagtaagacg tttattctcc cttttctttc gtattcccga
      961 tgataaaagg atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc
     1021 cgccagtgtg aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag
     1081 caacatgatg gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg
     1141 tacggcaagc ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa
     1201 cggcgatttg attcagggga gtccgctggc agattacatg tcggcgaaag gattaaaaac
     1261 aggtgatgtt catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact
     1321 cggcaatcat gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa
     1381 attcccttat gtaaatgcca acgtcattg
//
LOCUS       DAJSKJ010000313         1401 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000313 DAJSKJ010000000
VERSION     DAJSKJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.318"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..798
                     /locus_tag="ON345_003904"
     CDS             <1..798
                     /locus_tag="ON345_003904"
                     /inference="COORDINATES: protein motif:HMM:NF024700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3073876.1"
                     /translation="KNGDYISVYFDDSEEYFSDELKMAIDKSIHQTYHGYADLAWLRG
                     GDNVFYEGSFDSYLLMLRNEHLLKKAKIISGIDVFLSKDNNSVPLSHASSGELSFIAL
                     LVHIAFCINNRSFIFIDEPENSLHPKWQSEYFELLMGVIGYNECTTVVATHSPLIISS
                     ISESNQAAIYKREREGFVRVNSYDDNAEELYIDYFDTLTPKNRALSNRCVDIIDLFSV
                     GAISLNAAKERLSKYINMANDEVQKEFLIGVSGLLEEVASNKGPKHD"
     gene            791..>1401
                     /locus_tag="ON345_003905"
     CDS             791..>1401
                     /locus_tag="ON345_003905"
                     /inference="COORDINATES: protein motif:HMM:NF024787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3073877.1"
                     /translation="MTNIVFNVEELSLIRQAIGQGHKFWNDSSLTDLKRKIKDYLRQK
                     QVECCCYCSRNIDDEFNMVLDIEHIIPKSKIVTEMFEMENLAVSCKRCNMRIKGEDVS
                     FINGDFENFKQSGNYYQSQSYKFLHPNLDSWDDHLIYNVDQRNRRKIVYYHVVGNSVK
                     GAFTKKYFELDKVQVNTFDEAQDAGARKEPMDPFIAEKYSALLS"
BASE COUNT          439 a          193 c          310 g          459 t
ORIGIN      
        1 aaaaatggtg attacatttc agtgtatttc gatgatagtg aggaatattt ttctgatgaa
       61 cttaagatgg caatagataa atctattcat caaacatatc atggatacgc tgaccttgct
      121 tggttaaggg ggggggacaa tgttttctat gaaggaagct ttgattccta tcttcttatg
      181 ttaagaaacg aacacctttt gaagaaggct aaaataatat caggtataga tgtgtttttg
      241 agtaaagata acaattcggt tccgttgagt catgcaagtt caggtgagtt atcgtttatt
      301 gcattgctgg ttcacatagc attttgtatc aataatagat cttttatctt cattgatgag
      361 cctgaaaata gtttgcaccc taaatggcaa agtgagtatt ttgagctttt aatgggggta
      421 attggatata acgagtgtac tactgtcgtt gcaacgcact cgcctttgat tattagttct
      481 atttcggaaa gcaatcaagc ggcaatctat aaaagagaac gtgagggatt tgttagagta
      541 aattcttatg atgacaacgc ggaggagttg tatattgatt attttgacac gttgacaccc
      601 aaaaatcgcg cgttatccaa taggtgcgtt gatattattg atttgttctc tgttggggct
      661 atttctttga atgctgcgaa ggagcggttg tcaaagtata tcaatatggc taatgatgaa
      721 gttcaaaagg aatttctcat tggcgttagt ggtttattgg aagaggttgc aagtaacaaa
      781 ggccctaaac atgactaata tagtgtttaa tgtggaggag ctttctttga ttcgtcaggc
      841 cattggccag ggtcataaat tttggaatga ctcatctttg accgatctta agaggaaaat
      901 aaaggattat ttaagacaaa aacaagttga atgttgctgt tattgttcga ggaatattga
      961 tgatgaattc aatatggttc ttgatattga acatatcatc cctaaatcaa aaatagtcac
     1021 agaaatgttt gaaatggaaa atctagctgt ttcatgtaaa agatgtaaca tgagaataaa
     1081 aggagaggac gtgtctttta ttaacggtga ttttgaaaat ttcaaacaat ctggaaatta
     1141 ctatcaaagt cagagttata agtttcttca tccgaatctt gatagctggg atgaccatct
     1201 tatttataat gttgatcagc gtaatagaag aaaaattgtg tattaccacg ttgtcggcaa
     1261 tagcgttaag ggtgcattta caaaaaaata ttttgaattg gataaggtgc aggttaatac
     1321 ttttgatgag gctcaggatg ccggggctcg caaggagcca atggatcctt tcatcgcaga
     1381 gaaatattca gccttgctct c
//
LOCUS       DAJSKJ010000314         1399 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000314 DAJSKJ010000000
VERSION     DAJSKJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.319"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(1..199)
                     /locus_tag="ON345_003906"
                     /pseudo
     CDS             complement(1..199)
                     /locus_tag="ON345_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            complement(229..1349)
                     /locus_tag="ON345_003907"
     CDS             complement(join(229..1091,1091..1349))
                     /locus_tag="ON345_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="HCR3073878.1"
                     /translation="MKKRFSDEQIISILREAEAGVPARELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCD
                     ATGLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRR
                     EGLHVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALST
                     GRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTC
                     RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDW
                     RQDYNECRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
BASE COUNT          345 a          388 c          323 g          343 t
ORIGIN      
        1 aaacatgttg atgcaactca ttcaatatca tgtctctcgt atttaataaa ttgtagagca
       61 atttattatt tttatgtatg tatgcagatg aagccatgtc ttcgtgtttt aataattcac
      121 ataataaact ccaacccttt tccctgatgc ctatatgccc tgaaacgagc aatgcataaa
      181 gtacagcgcc aactcttcct gacctgcccc cacgattaga tacaacgctc agttagtaac
      241 gtcggaatct tcattctcag aatgaccctt tctccagccc gctgcaaatt cagacggtgt
      301 ctgataattc agcgtggagt gcgggcggca ttcgttataa tcctgccgcc agtcattaat
      361 aattttcctg gcatgaacga tatcgctgaa ccagtgctca ttcaaacatt catcgcgaaa
      421 tcgtccgtta aagctctcaa taaatccgtt ctgcgttggc ttgcccggct ggattaagcg
      481 caactcaaca ccatgctcaa aggcccattg atccagtgca cggcaagtga actccggccc
      541 ctggtcagtt cttatcgtcg ccggatagcc tcgaaacagt gcaatgctgt ccagaatacg
      601 cgtgacctga acgcctgaaa tcccaaaggc aacagtgacc gtcaggcatt cctttgtgaa
      661 atcatcgacg caggtaagac acttgatcct gcgaccggtg gaaagtgcgt ccatgacgaa
      721 atccatcgac caggtcagat tgggcgccgc cggacggagc agtggcagac gttctgttgc
      781 cagcccttta cgacgtcttc tgcgttttac gcccaggcca ctgaggtgat aaagccggta
      841 cacgcgctta tgattaacat gaagcccttc acggcgcagc aactgccaaa tacgacggta
      901 gccaaaacgc ctgcgctcca gtgccagctc agtgatgcgc cctgataaat gcgcatcagc
      961 agccggacgg tgagcctcat agcggcaggt cgacagggat aaacctgtaa gcctgcaggc
     1021 acgacgttgc gacagaccgg tcgcatcaca catcaacatc acggcttccc gcttctggtc
     1081 tgtcgtcagt actttcgccc aagagccacc tgaagcgcct ctttatccag cattgcttcg
     1141 gcaagcagct tcttgagtct ggcgttctct tcctcaagcg acttcaggcg cttaacttca
     1201 ggcacctcca taccgccata cttcttacgc caggtgtaaa acgtggcatc ggaaatggca
     1261 tgcttgcggc agagttcacg ggcgggtacc ccagcttcgg cttcgcggag aatactgatg
     1321 atctgttcgt cggaaaaacg cttcttcatg gggatgtcct catgtggctt atgaagacat
     1381 tactaacatc ggggtgtac
//
LOCUS       DAJSKJ010000315         1387 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000315 DAJSKJ010000000
VERSION     DAJSKJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.320"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(456..1103)
                     /gene="ychE"
                     /locus_tag="ON345_003908"
     CDS             complement(456..1103)
                     /gene="ychE"
                     /locus_tag="ON345_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR3073879.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQSAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT          446 a          307 c          269 g          365 t
ORIGIN      
        1 aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct cttttattga
       61 ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa
      121 tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg aaatgcacaa
      181 taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg gaattactaa
      241 tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa taacatgatg
      301 gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa gaatattttt
      361 acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat gacgaagtaa
      421 acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc ggggaaaatc
      481 cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag ccccataata
      541 cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg tgccatgcgg
      601 aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc aaacagatag
      661 ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc tggccccgcc
      721 atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt ttctgatttt
      781 tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat cgctatcgtc
      841 accaccagga tgcccccggc gatacggaac gaatcaattg atataccaaa aagttgcaga
      901 atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag gttggctgta
      961 aggttagttt tgtttcgcgc tgccgctgac tgataactgg tcatgctgat aaagacggga
     1021 ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa gtaaacggga
     1081 aaatcaaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata atgttagcca
     1141 taaataaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt tcacctccta
     1201 actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta
     1261 gccaccaaat catactacaa tttattaact gttagctata atggcgaaaa gcgatgctga
     1321 aaggtgtcag ctttgcaaaa atttgatttg gatcacgtaa tcagtaccca gaagtgagta
     1381 atcttgc
//
LOCUS       DAJSKJ010000316         1358 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000316 DAJSKJ010000000
VERSION     DAJSKJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.321"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..1358
                     /gene="espL4"
                     /locus_tag="ON345_003909"
                     /pseudo
     CDS             1..1358
                     /gene="espL4"
                     /locus_tag="ON345_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT          438 a          256 c          263 g          401 t
ORIGIN      
        1 atagtatgcc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
       61 aaaccccata aagatgatta ctcttcagct gaaaaatttc tcacgcactt caacaaaaaa
      121 tgcaaaaacc agactttggc gttggtttcc agccgtcctg aggggcgttg cgttgctgcc
      181 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      241 agtgttatgg cagtcatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      301 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      361 aacgataaaa taagaattat gagcgaaagc aaagagaata ttaaacacta ttctctgatg
      421 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatat tattaaccaa
      481 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggatctgtg
      541 gatgaaataa cccctccatt atcacctgca acgatgaatt tgctaatggc aattggtcag
      601 aatcaccaac ttacgcaact gatgattcaa ctccagaaaa tgccagaact acatagaaca
      661 gaaatgttga ctgcctataa tagtattaac ctgccaggtt tatatttggc tataaattat
      721 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
      781 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
      841 tgattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgcc
      901 ttacctaaac tggccgcaac acatcattta gataacgaac aagtgtataa attcctgagt
      961 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1021 atgctgaaaa ttgttttgaa cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1081 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1141 gcgatgcaaa atggacatag cgatatcgtg aaagttattc ttgaagcatt gcccagcctg
     1201 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1261 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1321 atttttaacg cattacccac tctttttaat acgtttaa
//
LOCUS       DAJSKJ010000317         1324 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000317 DAJSKJ010000000
VERSION     DAJSKJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.322"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>1324
                     /locus_tag="ON345_003910"
     rRNA            <1..>1324
                     /locus_tag="ON345_003910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          332 a          297 c          413 g          282 t
ORIGIN      
        1 agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt ggtcaggtag
       61 agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg gtgccgtaac
      121 ttcgggagaa ggcacgctga tatgtaggtg aagcgacttg ctcgtggagc tgaaatcagt
      181 cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag
      241 tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggtcagcgca
      301 agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg gtcctaaggt
      361 agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga tggccaggct
      421 gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca
      481 agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga gccttgatgt
      541 gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc gaccttgaaa
      601 taccaccctt taatgtttga tgttctaacg tggacccgtg atccgggttg cggacagtgt
      661 ctggtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga gcacgaaggt
      721 tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg cataagccag cttgactgcg
      781 agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg atccggtggt tctgaatgga
      841 agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata ccgcccaaga
      901 gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc tggggctgaa
      961 gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg ggtttagaac
     1021 gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg agaactgagg ggggctgctc
     1081 ctagtacgag aggaccggag tggacgcatc actggtgttc gggttgtcat gccaatggca
     1141 ctgcccggta gctaaatgcg gaagagataa gtgctgaaag catctaagca cgaaacttgc
     1201 cccgagatga gttctccctg actccttgag agtcctgaag gaacgttgaa gacgacgacg
     1261 ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct aaccggtact aatgaaccgt
     1321 gagg
//
LOCUS       DAJSKJ010000318         1296 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000318 DAJSKJ010000000
VERSION     DAJSKJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.323"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1225
                     /locus_tag="ON345_003911"
     CDS             <1..1225
                     /locus_tag="ON345_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3073880.1"
                     /translation="LGIPALRDRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAI
                     RTVKLQLTDCGETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKA
                     GHIDVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQ
                     RGRSTAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNM
                     DKTKITHVNDGFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSES
                     KVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIAFLMRW
                     WCKPPKPGQSKTWVLFGKTNHGKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNT
                     YTSRFTEVAMAFASI"
BASE COUNT          330 a          308 c          382 g          276 t
ORIGIN      
        1 actgggtatc cccgcgttgc gggatcgtat tgttcagcgg gccatgctga tggcgatgga
       61 gccgatatgg gagagtgatt ttcatacgct ctcatatggc ttccggcctg agcgcagtgt
      121 ccaccacgcg atccgcacgg tgaaattaca gctcacagac tgtggtgaaa cccggggacg
      181 ctgggtgatt gaaggcgacc tgtccagtta cttcgacacc gtacatcatc gactgctgat
      241 gaaggctgta cgccgcagga tcagtgacgc acgtttcatg actctgctgt ggaaaaccat
      301 caaggcggga catatcgatg tcggtctctt tcgggcggcc agtgaaggtg tgccacaggg
      361 cggtgttata tcgccgctat tatcgaacat catgctgaat gagttcgatc aatacctgca
      421 tgagcgctac ctgagcggga aagccagaaa agatcggtgg tactggaata acagtatcca
      481 acggggccga agtacggcgg tcagagaaaa ctggcagtgg aaacccgcgg tggcgtactg
      541 ccgctatgcc gatgattttg tcctcatcgt caaaggcacc aaagcacagg cggaagccat
      601 cagggaggag tgtcggggtg tgctcgaagg cagtctgaaa ctcaggctga acatggataa
      661 gactaaaatc acccatgtta atgacggctt tatctttctg gggcacagga tcattcgcaa
      721 acgcagtcgt tatggcgaga tgcgagtggt ctcaacgatc ccgcaggaga aagccagaaa
      781 cttcgccgca tcgctgacag cactgttatc aggcaactac agtgaaagca aagtcgatat
      841 ggctgaacaa ctcaaccgaa aactgaaagg ctgggccatg ttctatcagt tcgttgattt
      901 taaggccaaa gtcttcagtt atatcgaccg tgtcgtgttc tggaagctgg ctcactggct
      961 ggcccgcaaa taccgtacag gtatcgcttt cctgatgagg tggtggtgta aaccaccgaa
     1021 accgggtcag agcaaaacgt gggttttatt tggtaaaacc aatcacggca agctcagcgg
     1081 cgaaatactg taccggttgg tggggcaagg caagaagctg ttccgctggc ggctacccga
     1141 aggtaatccc tatctgagga cggagaccag aaacacgtat acatcgcgct ttacagaagt
     1201 ggcaatggcg ttcgccagca tttaaatgga gagccggatg cgctgaaagg tgcacgtccg
     1261 gttcggggag gagaggcagg gaaatagtcc gactac
//
LOCUS       DAJSKJ010000319         1273 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000319 DAJSKJ010000000
VERSION     DAJSKJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.325"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            18..777
                     /locus_tag="ON345_003912"
                     /pseudo
     CDS             18..777
                     /locus_tag="ON345_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(834..1190)
                     /locus_tag="ON345_003913"
     CDS             complement(834..1190)
                     /locus_tag="ON345_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3073881.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(1183..>1273)
                     /locus_tag="ON345_003914"
     CDS             complement(1183..>1273)
                     /locus_tag="ON345_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin HicA"
                     /protein_id="HCR3073882.1"
                     /translation="EPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
BASE COUNT          321 a          323 c          301 g          328 t
ORIGIN      
        1 gatacactgc aacatcaatg catgatcagc cattattgag tatgtccttg ccgtcgattc
       61 cctcttttgt gttgtcggga ttactgttgc tttgcttgcc gttttcttca tttgccagcg
      121 ccaccacatc acatatcact ttcagctacg ccgcccgcca gcggatgcaa aatcgtgcgc
      181 gtttattaaa acagtaccaa acccatctga aaaaacaggc cagctatatt gtggaaggca
      241 atgccgaaag cagaacacaa ccgcgaacag ataaaacagc atccagaatg gtttcctgcg
      301 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca tttcttaagc
      361 agcgaccatc tgcataacat caccgaagta gcgattcacc gtctggagca acagcttggc
      421 aagccttacg tctggggcgg tacgcggccc gatcaaggct tcgactgtag cgggttagtt
      481 ttttatgcct acaacaagat ccttgaggcg aaactcccac gcacggctaa tgagatgtat
      541 cactatcgcc gggtaaccat tgtggcgaat aacgacctgc gccggggaga tttgctattt
      601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
      661 tttattgagt caccgcgcac cggggaaacc attcgggtaa gtcggttagc tgaacctttc
      721 tggcaggatc actttttagg cgcgcggcgg attttgacgg aggagacgat tttgtaaatg
      781 ggtaatattg acgaactggc aaaaggtcgg atgccacgcg gcatccgacc tgtttacatc
      841 tgggctgaaa actcacgttc taagacatca acaatgagct ggttttttga aatggaatgt
      901 tgtctggcga cgatggtcag ccgcgaatca atgtgatgag gaacacgaag cgtcaggcgt
      961 ttttgctctt cttcttcacg aaaaggggca ataccgtcct cttcacaatc cgccatccac
     1021 tcatccagcg cgctttcgcc ttcacgatat aatgcctcaa tacttgtggc tacaaagtca
     1081 caataaccgt ttacattgag gaacttgccg cgaaatgcgt tgatatcggc ttcaaatgtt
     1141 actgcggctg gttgaccttt aattgtgata atgttcgatg ctttattcat aatctacccc
     1201 tacacagtta acaaaccact ccttaagacc tgctactgcg cctttatcca tcacattttt
     1261 tggatgtggc tca
//
LOCUS       DAJSKJ010000320         1268 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000320 DAJSKJ010000000
VERSION     DAJSKJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.326"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..718)
                     /locus_tag="ON345_003915"
     CDS             complement(<1..718)
                     /locus_tag="ON345_003915"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCR3073883.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAAKPEPHWAIPQGQSAHDTF"
     gene            901..1164
                     /gene="cedA"
                     /locus_tag="ON345_003916"
     CDS             901..1164
                     /gene="cedA"
                     /locus_tag="ON345_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR3073884.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          305 a          290 c          332 g          341 t
ORIGIN      
        1 agaaagtatc gtgggcactt tgcccttgtg gaattgccca gtgcggttct ggttttgccg
       61 catgaacaaa atcggggaat ttatgcgcat cctggataaa gaagattggc gtgttattgc
      121 caacgaggtc aaaaataccc tcttcagtat agaacttggt ggcaaaacca cggatatcac
      181 gcacggtatc ggcagagcca gcgccaccct gaacggtaga gaacctaaca aatactgggg
      241 tgattttgtt cggatctgag aggaaatccg ctttggtgat atcgcttaag cttttatatg
      301 gctggaaata accgtgagct gctgatccgc gtgcatgaac aatacgttcc gggatgcgct
      361 catggtcaaa gtgggtgatt ttctcgcgca gaataaaatc ttccagcagc gttggaccac
      421 ggttaccggc acgcagtgag ttttgatcat cggcgatgcg cacgccctga ttagtggtca
      481 gcgcataatt ttcactgcct ttgcgtacgt cttccagaga attaagtttt tcgttacgtg
      541 tatcaggggc tttcaggctc ccaggggcgg taggttgtgc acctggcggt gttggttcag
      601 ccgctggacg atgagatccg tcctcaggtg ccagtgagtc catccccggt ttcgcttcgc
      661 tggaatcgtg tagtggtgac tggtgctgat gtgggttctt ttcgttatgt tgcgacattg
      721 aactcgtctc cttaatttat tactgaaagg gccgctgttt tggtcaaaaa actctctaac
      781 tatagaccac cagccagatc ccgcttcgga gactattctg gaaacggtgt tatgaaatac
      841 acgcaaagcg ggacgtacag gggctaaatc ggctaaacta cagtttttct aatttttaat
      901 atgcgtttag tgaagccagt aatgaagaaa ccgctccgcc agcaaaaccg ccagattatt
      961 agctatgtcc cacgcacgga acccgcgccg ccagaacatg cgataaagat ggattcgttt
     1021 cgtgatgtct ggatgctgcg tggcaaatat gttgcgtttg tactgatggg agagtcattt
     1081 ctgcgctcac cggcgtttac tgtgcctgaa tctgcccaac gttgggcaaa tcagatccgc
     1141 caggaagggg aagtgactga gtaaggatta catacctccc ggttttgccg ggagttacat
     1201 gacctgaaat tctgcatttc gttgctacat tcattcaaac cacctgtaac aaaaagctgc
     1261 ctttgacg
//
LOCUS       DAJSKJ010000321         1259 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000321 DAJSKJ010000000
VERSION     DAJSKJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.327"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..1251
                     /locus_tag="ON345_003917"
     CDS             <1..1251
                     /locus_tag="ON345_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3073885.1"
                     /translation="VRFIQQSPLTLSNGAKVILAKSAGEEQKLQITAVSNKGDLSFPA
                     QQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPE
                     PGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKL
                     LQENQIVQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVTLITRYLGSIKHSDSP
                     LAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKD
                     LRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAK
                     GISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVE
                     NVNTAVKQYLSHPVNTYTGVLLPK"
BASE COUNT          375 a          292 c          284 g          308 t
ORIGIN      
        1 gttcgattta ttcaacaaag cccattaaca ctttccaatg gtgccaaggt tattctggca
       61 aaatccgcgg gtgaagagca aaagctacaa attactgccg tatctaataa aggcgattta
      121 agtttccctg cgcagcaaaa atcacttatc gcgctggcaa ataaagcagt aagcggaagc
      181 ggcgttggtg agctctcctc ttccagcctg aaacgctgga gtgcggaaaa ctcggtaacc
      241 atgagcagta aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg gactaataac
      301 cctgaacctg gttttcagtt gattaaccag cgaatcaccc acagcacgat taacgacaat
      361 atatgggcat cgctacaaaa tgctcaaatt caggcgttga aaacgctcga ccagcgtcca
      421 gcggagaaat tcgcccagca gatgtatgag acgcgctatg ctgatgaccg cacgaaatta
      481 ctgcaagaaa atcagattgt acagtttact gccgcagatg cgctggctgc cgatcgccag
      541 ttgttttcat ctccagcgga tatcacgttt gtcattgtcg gtaatgtcgc agaagacaaa
      601 ctcgtgacgt taattacgcg ttacttagga tcaatcaaac actctgattc gccattagcc
      661 gcaggtaaac cattaactcg cgcgacggac aacgcatcgg ttactgtaaa agaacaaaat
      721 gaacctgtgg cacaggtttc acagtggaag cgttatgatt cccggacacc tgttaatctg
      781 gcgacgcgta tggcgctcga tgcttttaac gtcgcactgg caaaagatct acgcgttaat
      841 attcgtgaac aggcatctgg agcatacagc gtttcttctc gcctctcggt tgatcctcag
      901 gccaaagata tcagtcattt gctggctttt acttgtcaac cagaacgaca tgatgaactg
      961 ttaacgttag cgaatgaagt gatggttaag cgtctggcta aagggatcag tgagcaagaa
     1021 ctgaatgaat accagcaaaa cgttcagcgc agcctcgata tccaacagcg tagcgttcaa
     1081 caattagcga acactattgt aaatagtctt attcaatatg acgatcctgc agcatggact
     1141 gagcaggagc aattgttgaa acaaatgacg gtagagaatg ttaacactgc cgttaaacaa
     1201 tatctttctc atccggtaaa tacttatacc ggagtattat tgccaaaata ataacagcc
//
LOCUS       DAJSKJ010000322         1233 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000322 DAJSKJ010000000
VERSION     DAJSKJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.328"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..470
                     /locus_tag="ON345_003918"
     CDS             <1..470
                     /locus_tag="ON345_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR3073886.1"
                     /translation="DADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLP
                     ENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKE
                     LGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            486..>1233
                     /locus_tag="ON345_003919"
     CDS             486..>1233
                     /locus_tag="ON345_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR3073887.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGRLSFYEINCPTVNNSCNVSVAKRDK"
BASE COUNT          413 a          255 c          251 g          314 t
ORIGIN      
        1 tcgacgcaga tggtgcagaa gatattccgt tgaacaataa acgcgcggta actaatcggt
       61 atggcatcgg agttattcca tcagtcagca gttacataac aacatcatta agcgttgata
      121 cccgaaatct tccagaaaat gtggatatcg aaaactcggt tatcaccacc accttaaccg
      181 aaggtgctat tggctacgcc aaacttgata cccgcaaggg ctatcaaatc atgggggtta
      241 ttcgcctggc agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc
      301 ataaagaatt aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg
      361 acagcaaact tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca
      421 gcaacttaac caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga
      481 aactaatgcg ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca
      541 tgagcagcat tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt
      601 cagataaagg ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc
      661 aaacatggat agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc
      721 ctatggtacg tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa
      781 ttgctcaact gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc
      841 caaaaacaga taaaaaaaat gtgatgcaag tcactatgca gcacgcattg aaactattct
      901 ggcgaccaaa agctattgaa ttagaagatg atggggtaat gtcttacgaa aaagttgaaa
      961 tcatccgtag aaatgatggt tcgatccgct ttaacaataa gatgccttat cacgttacgc
     1021 tgggttatat cggaacaaac ggaatcacaa tgttgccaca aacgcaaagt ttaatggtaa
     1081 ccccttatag ccatgcagat acgcagttta aaaatgttcc gtcagctttt caggttggtt
     1141 atatcaatga ttttggtaga ttaagttttt atgaaataaa ctgcccgacg gttaataact
     1201 cttgtaatgt ttccgtagcc aaacgagata aat
//
LOCUS       DAJSKJ010000323         1230 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000323 DAJSKJ010000000
VERSION     DAJSKJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.329"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(577..1149)
                     /gene="hdeD"
                     /locus_tag="ON345_003920"
     CDS             complement(577..1149)
                     /gene="hdeD"
                     /locus_tag="ON345_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR3073888.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLSATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          420 a          269 c          238 g          303 t
ORIGIN      
        1 caaaaagtta acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga
       61 tctctgaaga atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt
      121 ttcgacttaa gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag
      181 taaataacgc attcatgtct tcaggtcttt atatagcgtc tggagacacg gatatttatg
      241 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
      301 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
      361 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
      421 aaaaattaga tgaaattcag taggttgaaa taatcactag caggtaatta tttcaatgat
      481 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
      541 atcaagtgac tatgatccgg gtgacaatcg gggtaattat tgctgcttaa cgaacaaact
      601 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
      661 cagcgttaca gacaccatcg gcgttgcact aaggaatatc caggcaatga cgatatccag
      721 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
      781 caggcgaata actcctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
      841 gcccagctcc ggtgcgcgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
      901 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag
      961 cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac
     1021 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
     1081 tgctctgcga tgttttttaa gcatctccag atcaaacttc aaaattgttg ccttatctat
     1141 atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc
     1201 aaaaacgcgt ctaagaatgc agtcgattta
//
LOCUS       DAJSKJ010000324         1225 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000324 DAJSKJ010000000
VERSION     DAJSKJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.330"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(157..230)
                     /locus_tag="ON345_003921"
     tRNA            complement(157..230)
                     /locus_tag="ON345_003921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Gly,seq:ccc)
     gene            complement(309..1064)
                     /locus_tag="ON345_003922"
     CDS             complement(309..1064)
                     /locus_tag="ON345_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3073889.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          317 a          296 c          289 g          323 t
ORIGIN      
        1 ggttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa atggggaagt
       61 gttacagcga ttgcgaaaag cctgaagcga ttgaattaag ataatataga aaatcagata
      121 cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag ggaatcgaac
      181 cctcgtatag agcttgggaa gctctcgttc taccattgaa ctacgcccgc ttcgagatgc
      241 gtaaggcatt ataaacctta cgctctcctt agcaagtgcc gcgctgctga ctgctgatta
      301 attcgccatc agcattttgg cttgctgccc tgcggcggca agtaacgtag cggatcaatt
      361 gccgttgcac ggtaacgaat ctggaaatgc aggcgaacag atgccgcatc cgtgctcccc
      421 atagtggcga ttttttgccc agccttcacg ctttgcccat tatttaccag catcgtgtca
      481 ttatgggcgt aagccgtaat gtaatcttca ctgtgtttaa tcatgatgag attaccgtag
      541 ccacgcagct ggttgcccac atacaccacc tttcctgcac ccgcggcgta aataggtgta
      601 ccccgtggag ctgagatatc aatcccttta ttgccgccat ctgctgtcga atacggcatg
      661 ataactttcc ctgtcgttgg ccataaccaa caacgttgcc ctactggcgg ccaggaagat
      721 ttcggtaccg ctgatgacgg tgtaaccgat gcggttttgg tcgttgattt ggcggttgat
      781 ttacgtgtac tactgctact tttcgcccca cccagtttta gtttctgacc aacttcaatg
      841 gtgtaagggg gggaaatgcc gttcagtcgc gccagttctt ttacgctggt tcccgtggtg
      901 cgcgaaatac gatatagcgt atccccccgt ttcacggtgt aaacggagcc ggaatacgtt
      961 cctgtatcgg atgatttgct acccgaacag cccgccaaaa gcagtccaac cgataacaac
     1021 atcacgatac ccagagattt tttattcagg cgtcccgcac tcaaaatctt tcctcgctta
     1081 aaaaacaaga cgccttatca tagcagccag cctcttgata cccaatctct tgcttctgga
     1141 aaaacgtggt aatagagaaa gaggttaatt tcatttggca aagggaagta tcatcagcaa
     1201 aatgttcgct gtagaggtat cgaag
//
LOCUS       DAJSKJ010000325         1221 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000325 DAJSKJ010000000
VERSION     DAJSKJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.331"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(114..770)
                     /locus_tag="ON345_003923"
     CDS             complement(114..770)
                     /locus_tag="ON345_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HCR3073890.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
BASE COUNT          359 a          264 c          270 g          328 t
ORIGIN      
        1 aagacgcctg agggtaaaaa tttatacatg gttgtcccta tgtctattat tgcaaggttg
       61 acaatattat tcgttttata aatttgagcg tttaaaatca cacatcaaag caattagtca
      121 ggataaacaa caatacccag tgcttcgaga atcagttctt gctgccaggc tgcaattttg
      181 atacctgatg tatcgacttt gcggggatct aaaccgtcga gttcggcgtg gcataaattg
      241 gcaccctgta ggctaaattg cccccagaca tcttcggaaa aaacacctcg acttaagtct
      301 gactctttca gtgatgcacc cgctaggttc gttcctatcc agcgattttc aaacaactca
      361 catttttcta aacaaaccct ctccatattg gcataggaaa gattacatcc agaaataaat
      421 gctgagcaaa agtacatacg attactcact tgatgggcaa agtttgttcg ggaaaagttg
      481 gcacctttta aatcacacgc acggaactct ataccgtagc aattggcatt actgaagttt
      541 gccatcgcaa gttggcattg ttggaaactt gcatcacgaa gatctgcgac atcaaagtgg
      601 cagccttcga tatcaccctg ttcaatgaac ttgcagttga cgaatgtcgt atcacgcaag
      661 ttagcacgtc gaaagtcgct gcgaatgaat gtgcaagcgg tgaaggtgag atcacttaag
      721 tctttatgtg aaaagttgtg gtgccgatat gtatgattgt aggtttccat gagtactcct
      781 tgttgaaagg agggctagac taccattata catatgccaa gacattaaaa taaaagcctg
      841 ttacatcaac acattacaac ccctcaattt acctgtttta agtggtcaaa ttggcgcgta
      901 gagcgcctat cgctcttgca ttggcacgcg gccctgttga aaaaagttat tcgctttaga
      961 tagggttagg agagccgtgc ttcgccggta aaaaatcgga ctatccgacg cagggcggac
     1021 tcatcggcgg agaagcaaac tttgactata tctccctaca cttggaagag ggctagactg
     1081 gccccataga tcaccagaca cttcgactct cttaaaataa gaggtagtct tggaactatg
     1141 tttaatacca gtcagaacga taagatggtt ggagtgttat caggacctga gcggcgtcgg
     1201 cgtcgtacac cgcaggaaaa a
//
LOCUS       DAJSKJ010000326         1218 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000326 DAJSKJ010000000
VERSION     DAJSKJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.332"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(30..935)
                     /gene="feaR"
                     /locus_tag="ON345_003924"
     CDS             complement(30..935)
                     /gene="feaR"
                     /locus_tag="ON345_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCR3073891.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDV
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
BASE COUNT          330 a          295 c          278 g          315 t
ORIGIN      
        1 ggcgttgcag tacacacaac gccaattagt tagcggaatt tacgtcgata ctcgcctggc
       61 gtcatcccaa agcgttgctt aaataccgtt gaaaaatgac tctggtcaga aaatccccaa
      121 tgaaagccga tgcctgccag tttttcatca tctgcggcat ggcgaatcgc atctgcacaa
      181 aaatcgagac gacggttacg aatatattgc gcgaccacca aacctttatc ggcaaacatt
      241 cggtacaaac tacgtactga catacctgtc tctccggcta tccactccgg gcgtaatatc
      301 tcttcgcgaa tattatcgtc tatcaacgta accacttttt gaaactgacg ttcacgacga
      361 ggttgaacag attcccgctg atgaagtacc gggcgcagca gacacaccat cgcctgtagc
      421 gcagcttcac tttctgtttc agaaagtgcc ggattattca tgctctcctg taacaggcga
      481 tgactgagtt gcaccatggg taagtcagcg tccagtcttt ctgcgcagac aggtttttga
      541 tggggaaaat attgttccag cagagtgcgt ggcaaaagta atgaaatctg tttagaagac
      601 tcctgccagt aaagcgaaca ggggcgtgag acatcgagta acgtaatatc gccagcgcca
      661 atctgcacct gacgctcatc ctgctccatt attgcctgac cactaagctg aaaaacggtg
      721 taaaaccagg catcgtcgct gccttttact tcctgccagg tgcgggataa attcaccccg
      781 ctggttgtca cggtactcag ctttagtcct ttggcaaaat gcgtatccag tacacccgtg
      841 taacgctcag tcagcaggtg tccggtaaaa tttccgcata cctgattgat ttgggaaagc
      901 cattgctgaa actcattatc cactgcgggg ttcatggcac gttttcgctc tgtgaaatgt
      961 atttttattg ttgcatttgt gttgcagtaa acgaagctaa tgagcctgac tataggaaat
     1021 aagtcttctc aggcatagag acataagcgg ttattgtcac gatttgcgga gcttgtcaca
     1081 gctgacaaag cgaatgtcac agcgaaaaaa gtgacttttc ttgtcgctgc gtacactgaa
     1141 atcacactgg gtaaataata aggaaaagtg atgacagagc cgcatgtagc agtattaagc
     1201 caggtccaac agtttctc
//
LOCUS       DAJSKJ010000327         1210 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000327 DAJSKJ010000000
VERSION     DAJSKJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.333"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..106)
                     /locus_tag="ON345_003925"
     CDS             complement(<1..106)
                     /locus_tag="ON345_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3073892.1"
                     /translation="MKQLQAFKFQLRPGGQQECEMRRFAGACRFVFNRA"
     gene            114..620
                     /gene="tnpA"
                     /locus_tag="ON345_003926"
                     /pseudo
     CDS             114..620
                     /gene="tnpA"
                     /locus_tag="ON345_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(638..>1210)
                     /locus_tag="ON345_003927"
     CDS             complement(638..>1210)
                     /locus_tag="ON345_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR3073893.1"
                     /translation="GFLNNYSDLKETTSATGKPVLRWVDPSFDQSKYDSIVWNPITYY
                     PVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKE
                     GLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLN
                     NESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT          314 a          285 c          273 g          338 t
ORIGIN      
        1 gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cactcctgtt
       61 gaccacctgg tcttaactgg aatttaaatg cctgcaattg tttcacaggt caaatgatac
      121 tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc
      181 atgttcacct ggtctttgtc accagatacc gacgccagat ttttgaccat gacgcgacag
      241 aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa ctggttgaaa
      301 tggatggcga accagatcac gtccatttgt taattaacta acctcccaaa ctggcgatat
      361 ccagtctggt aaacagcctc aaaggcgtat cgggtaggtt actgcgacga gatcgaccag
      421 atattgcagt caggtattac tacaaaggcg ttttgtggag tgctggctat tttgccagta
      481 gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg
      541 gtcaggtgga aagccgcgcc ttatatcccc gccctgaagg atggggtttt acggcgcacc
      601 ggataaaaag cgccatctaa cgatggcgcg tctggaacta ctttttgtta acgtcaaaca
      661 tggtggcatc ggtcgccatg tcatcaataa cttgtttaat attttcgaaa gccattggcg
      721 tactttcgtt attcaggtct ttaccttcgc cctgacgaac gactttgata accggtttat
      781 tagtcgctgc atcaatcagc tcaccttcaa aatagaggcg agtatccatg gtacggtggc
      841 ctgtagccat ttgcgtcccc gcaaccacta atgcaacagg aaccacttca tagaattgca
      901 gcccttcttt gctggtatct acaccggtaa tggccccacg gaaaatcaga ctacgcggcc
      961 cagcggtggt aaccagtggt ttacgctgcg ctatcgcttc tttcatttcg gtgttggtat
     1021 agttcaaaat tttatccaga actttctgcc ctacctgggt agaaggtttt ggcaccggat
     1081 aataagtgat tgggttccag acgatgctgt catatttgct ttgatcaaaa ctcgggtcta
     1141 cccaacgtaa aacaggttta cctgttgctg aggttgtttc ttttaaatca gagtaattgt
     1201 ttaaaaaacc
//
LOCUS       DAJSKJ010000328         1195 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000328 DAJSKJ010000000
VERSION     DAJSKJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.334"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            130..633
                     /locus_tag="ON345_003928"
     CDS             130..633
                     /locus_tag="ON345_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR3073894.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(712..963)
                     /locus_tag="ON345_003929"
     CDS             complement(712..963)
                     /locus_tag="ON345_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR3073895.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1078..1164)
                     /gene="azuC"
                     /locus_tag="ON345_003930"
     CDS             complement(1078..1164)
                     /gene="azuC"
                     /locus_tag="ON345_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR3073896.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          318 a          279 c          276 g          322 t
ORIGIN      
        1 cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc
       61 tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg
      121 atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag
      181 ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac
      241 ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg
      301 tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttcccggt
      361 gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt
      421 tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc
      481 aatttcctgc gcgatctgga aaaagaacag cagcatgatg gtctgttgct gcaaaccatt
      541 cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat
      601 gttctgaatg tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat gcattgcgcc
      661 gatgaaggtt ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt tttaatggga
      721 ttcaccctgt ggggtaaact tgagttcaat aagcgcgatg gctttttgga ttgcccgcat
      781 ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttca gctgactgga
      841 cattttttca cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat
      901 aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca gcggctggga
      961 catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcagc
     1021 gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccggagcagg ttttttatta
     1081 tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact
     1141 tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac aggtg
//
LOCUS       DAJSKJ010000329         1189 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000329 DAJSKJ010000000
VERSION     DAJSKJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.335"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            133..240
                     /locus_tag="ON345_003931"
     CDS             133..240
                     /locus_tag="ON345_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073897.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(293..1051)
                     /locus_tag="ON345_003932"
     CDS             complement(293..1051)
                     /locus_tag="ON345_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR3073898.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          330 a          290 c          278 g          291 t
ORIGIN      
        1 ttttcataaa acgcagaaac agtgaccatg tgcacaacgc cacccggcgg ctgtaataac
       61 aaaaaatccc ttgatgcctg tcccttttgt tacactccgt tatcacgcac aagagatatg
      121 caggacactg gtatgccgac taaacgcttt gataaaaaac actggaagat ggtggtggtg
      181 ctactggcaa tctgtggcgc tatgttgttg ctacgttggg cagcaatgat ttggggctga
      241 gcagtgacaa acgaagacag ccagaccgca taacacggtc cggcagataa ttttacatgc
      301 tgtagcccag cgataaggtt gtccgacggt cggtatgttc cggtgcagac tctggaggtt
      361 cagagttcca tgtgacgttg taagccacct tcagtccaaa atgttcatta atagcaacat
      421 ttaatgcgct ttcggagttc agcgttgtgt cttccgcgcc aaagaccgaa acaccctgcg
      481 taaatttagc gttgtcagtc aactgccatg cataagcccc ggaggcatag cccagcggct
      541 gggtttcact ggcattgtcg gtatatttgt cgtaacgcac acctggaccg aattcaaagc
      601 ggaaactgtg taccgggcca ttcaaaaact gacgaccata acccgcggtc aacacatcgc
      661 gctctcgata gccgttataa cggtctgtca gccagcttgc ctgaccaaat aaatagtcat
      721 aatcagttaa attaaagcgg ctacgcccgc ccgccgcata tttttctgaa gaacgctcat
      781 cattagaaga agtattactg gcgttccccc acagcgacca ggcagtggtt tgtccatacc
      841 aggtcatggt ggtgtcagcc gtaagtgagg agcttttcgt attgcctgat tgtgcaagat
      901 atcctgcgtt cagattacct tcgaaaggtt ttttagcgct ggcaggatcg tccatgacag
      961 taaaaacgga atcatcggca gctgcattca gtgacgcaaa catgcccccc gccaacataa
     1021 cgatggcagg aactgtcttc aaaagcttca tttatcaaga gtccgtacaa caaaaaaaga
     1081 gaccatcgcg gtcccggaaa ctttcttaag gatcaaagat tagcgtccct ggaaaggtaa
     1141 cgaattataa aaaggcgcga ataacttagc aatgtattct tatttcatt
//
LOCUS       DAJSKJ010000330         1158 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000330 DAJSKJ010000000
VERSION     DAJSKJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.336"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(254..949)
                     /gene="aqpZ"
                     /locus_tag="ON345_003933"
     CDS             complement(254..949)
                     /gene="aqpZ"
                     /locus_tag="ON345_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR3073899.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          347 a          313 c          277 g          221 t
ORIGIN      
        1 gttttacagg gatgttacct cgcgcccgac ccggtagccg taagggataa gtcgagggca
       61 ccgcgcagcg gcgattttgt tcgccagagc ccgggggtgc agggggcggc ggcgattggc
      121 cgccccctgc gcgctccttg cgccagtggc aatatgtggc ttagctcatg aaaggagcgc
      181 aacaagatga tgaatcaaca tataacaaca tcttaaaaaa aggcctgaca ttacgccagg
      241 ccttctgcgt taattaatca cgcttttcca gcagggtccg gtaaatcaga ccaccgataa
      301 tgccgccgac aattggcacc acccagaaga accacagttg ttctaatgcc cagccgccct
      361 ggaagatagc aaccgcggtg ctgcgcgccg ggttaacaga agtgttagtc accggaatac
      421 taattaagtg aatcagggtt aaggccagac caatagcgat cggcgcaaaa cctgccggcg
      481 cgaatttgtc ggttgcgccg tggatcacca acaggaaacc tgcactcaat accagttcaa
      541 ctaccagcgc ggaaagcatg gaataaccgc ctggtgaatg ctcgccataa ccgttagaag
      601 caaaaccgct ggctgccgcg tcaaaacccg ttttaccact ggcaattaaa tacagcagcg
      661 ccgctgcaac aataccgccg acaacctggg caattacgta gccaacgact tcttttgccg
      721 gaaaacgtcc gccagcccat aaaccaatag tgaccgccgg gttaaaatga ccaccagaaa
      781 tatgaccaac agcaaaggcc atcgtcagaa cggtcagacc gaacgccaac gccacgccgg
      841 caaaaccaat gcctaattcc gggaagcctg cggccagtac agcactacca cagccaccaa
      901 aaacaagcca gaaagtacca aaacattcag ctgctaattt tctgaacata tccacctcaa
      961 ttaaaaattg accctgtgaa aaatatggtc gttttatagg gtcgtcgtaa aaaagtgacg
     1021 acggaaataa tgcgcggcta ttttaaaaac gaaggcgagt cattcaccag ataaataaat
     1081 ccagtaaatt tgatttaggg caacagcggg ttgccccata tagtcatttg tctgattgac
     1141 agtgtagtgc aagcaaaa
//
LOCUS       DAJSKJ010000331         1154 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000331 DAJSKJ010000000
VERSION     DAJSKJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.337"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            48..1028
                     /locus_tag="ON345_003934"
     CDS             48..1028
                     /locus_tag="ON345_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HCR3073900.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGRVKTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
BASE COUNT          314 a          320 c          304 g          216 t
ORIGIN      
        1 gatatgagat catcatattc atccggagcg catcccagag ggacatcatg agccatcaac
       61 tcaccttcgc cgatagtgaa ttcagcacta agcgccgtca gacccgaaaa gagattttcc
      121 tctcccgcat ggagcagatt ctgccatggc agaatatgac cgctgtcatc gagccgtttt
      181 atcccaaggc gggcaatggc cgacggccct atccgctgga gaccatgctg cgtattcact
      241 gcatgcagca ttggtacaac ctgagcgacg gtgccatgga agatgccctg tacgaaatcg
      301 cctccatgcg cctgtttgcc cgattatccc tggatagcgc cctgccggac cgcaccacca
      361 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
      481 ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc
      541 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
      601 ccaagagtgg cctgacccac agcctagtca ccaccgcggc caacgagcat gacctcaatc
      661 agctgggtaa tctgcttcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
      721 gagcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgtc
      781 ccggcagggt aaaaaccttg aagcagcatc cgcgcaagaa caaaacggcc atcaacatcg
      841 aatacatgaa agccagcatc cgtgccaagg tggagcaccc gtttcgcatc atcaagcggc
      901 agttcggctt cgtgaaagcc agatacaagg ggctgctgaa aaacgataac caactggcga
      961 tgttattcac cctggccaac ctgtttcggg tggaccaaat gatacgtcag tgggagagat
     1021 ctcagtaaaa accggaaata acgccagaaa tggtggaaaa aatagcctaa ataggctgat
     1081 tcgatgtgtt tgcgggaaaa aaatcggccc agatccgcga aattttaatc agcgagtcag
     1141 cttgggaaga aatg
//
LOCUS       DAJSKJ010000332         1143 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000332 DAJSKJ010000000
VERSION     DAJSKJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.338"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..176)
                     /locus_tag="ON345_003935"
     CDS             complement(<1..176)
                     /locus_tag="ON345_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3073901.1"
                     /translation="MPRKGFKINEEINITVLDNNHNENESVESEENGTHTENINKNHV
                     AKGIFTTKNTLLIV"
     gene            191..832
                     /locus_tag="ON345_003936"
                     /pseudo
     CDS             191..832
                     /locus_tag="ON345_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          236 a          240 c          267 g          400 t
ORIGIN      
        1 caaactataa gtaatgtatt ctttgtggta aatatacctt ttgcaacgtg atttttattt
       61 atattttcag tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta
      121 ttgtcgagaa cagttatgtt gatttcttca ttgattttga atcctttccg tggcaccgtt
      181 ctgataactg atcctaccca cgtaatatgc gctgttcctg atttcgttac ttgggccgtt
      241 ggtcatcttt ctgctgtttc ggatgatccg caccagttat gtcaagcagg tcgctatttc
      301 agtagcgaaa aaagtaaaat ataaaagtct gactcgctat gtgctgcttt cggtgtgcat
      361 ctcaacggtt gttaacctgc ttactatcag cccgttgcgt aacagtgatt cttttgtgac
      421 agaggggcag tggttaacgt ttaaatcgat aattgcattg ctcattcttt gtggcgtagt
      481 gttggcgatt aatctgtttt ttctgcgctt ctccaagcgg tacgcttttc tgggcaggct
      541 ttttttgcag gaaatcgatc tgtttttctc cagtgaaaat gcgttgtcga ccttttttgc
      601 caagccgctt tggcttcggt tattcatatt gctggttatt gaaatgatgt ggattacgct
      661 ggtgtcggta ttggcaacgc ttgtggaatg gcggatttgg tttgaagcct attttttact
      721 ctgctatgta ccgtgcttaa tttactattt tttccattgt cgattcctct ggcataacga
      781 ttttatgatg gcatgtgaca tgtatttccg ttgggggcat tttaataagt gaggaagtta
      841 caggaagtga ccagataacg catatatgtt ctctactatc ttgcgcattt tgattgttga
      901 ctgagtaacc agacagttga tgtgcacaat ttcccctcgc cctaacagac gtgggcgggg
      961 gcacccggtg tgtgcaatat acgaaaaaaa agcccgtact ttcgtacggg ctcttcttta
     1021 aatatggcgg tgaggggggg attgactcgc ttcgctcgcc ctgcgggcag cccgctcact
     1081 gcgttcacgg tctgtccaac tggctgtcgc cagttgtcga accccggtcg gggcttctca
     1141 tcc
//
LOCUS       DAJSKJ010000333         1122 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000333 DAJSKJ010000000
VERSION     DAJSKJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.339"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..267
                     /gene="tnpB"
                     /locus_tag="ON345_003937"
     CDS             <1..267
                     /gene="tnpB"
                     /locus_tag="ON345_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3073902.1"
                     /translation="AKVQTALKDDPMSGHVFIFRGRSGSQVKLLWSTGDGLCLLTKRL
                     ERGRFAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTML"
     gene            317..>1122
                     /locus_tag="ON345_003938"
     CDS             317..>1122
                     /locus_tag="ON345_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3073903.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDIAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGITGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWA"
BASE COUNT          237 a          322 c          341 g          222 t
ORIGIN      
        1 gcgaaagtac agacggcgct gaaagacgat cccatgtccg gccatgtttt cattttccgg
       61 ggccgcagcg gcagtcaggt taaactgctg tggtccaccg gtgacggact gtgcctcctg
      121 accaaacggc tggagcgtgg gcgcttcgcc tggccgtcag cccgtgatgg caaagtgttc
      181 cttacacagg cgcagctggc gatgctgctg gaaggtatcg actggcgaca gcctaagcgg
      241 ctgctgacct ccctgaccat gctgtaaatc tctttatcct ggttgtcaca gaataagccc
      301 ggtaaaatac gggcttatga acgacatctc ttctgacgac atcttcctgc tgaaacagcg
      361 cctggccgaa caggaagcgc tgatccacgc cctgcaggaa aagcttagca accgggagcg
      421 cgaaatagac catctgcagg cgcagctgga taaactccgc cggatgaact tcggcagtcg
      481 ttccgaaaaa gtctcccgcc gtatcgcaca aatggaagcc gatctgaacc ggcttcagaa
      541 agagagcgat acgctgactg gtagggtgta tgacccggca gtacagcgtc cgttgcgtca
      601 gacccgcacc cgtaagccgt tccctgaatc actaccccgt gacgaaaagc gactgttgcc
      661 tgcggcgccg tgctgcccga actgcggcgg ttcactgagc tatctgggcg aggatatcgc
      721 cgaacagctg gagttgatgc gtagcgcctt ccgggttatc cggacggtac gggaaaaaca
      781 tgcctgtact cagtgcgatg ccatcgtgca ggcacctgca ccttcgcggc ccatcgagcg
      841 gggtatcacc ggaccggggc tgctggcccg cgtgctgacc tcgaagtatg cagagcacac
      901 cccgctgtat cgccagtcag aaatatacgg ccggcaaggt gtggagctga gccgttcact
      961 gctgtcgggc tgggtggatg catgctgccg gctgctgtct ccgctggaag aggcgcttca
     1021 tggctatgtg ctgactgacg gtaagctcca tgttgatgac acgcctgtcc cggtgctgtt
     1081 gccaggcaat aagaaaacga agaccgggcg gttgtgggcg ta
//
LOCUS       DAJSKJ010000334         1094 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000334 DAJSKJ010000000
VERSION     DAJSKJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.340"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            72..1046
                     /gene="cysB"
                     /locus_tag="ON345_003939"
     CDS             72..1046
                     /gene="cysB"
                     /locus_tag="ON345_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR3073904.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFTRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          285 a          262 c          265 g          282 t
ORIGIN      
        1 aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg
       61 gatgatttaa catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc
      121 tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc agtaaacaag
      181 tcagaatgct ggaagacgag ctaggcattc aaatttttac ccgaagcggc aagcacctga
      241 cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag
      301 tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata
      361 tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg
      421 agcgttatcc ccgcgtttct ttgcatatgc accagggctc gccgacacaa attgctgatg
      481 ccgtctctaa aggcaatgct gatttcgcta tcgccacaga agcgctgcat ctgtatgaag
      541 atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc
      601 cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat
      661 ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa
      721 cgccgcgtat cgtcttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag
      781 ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg
      841 tgcgtgttga tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta
      901 gtactttctt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc
      961 gtgatgtcgt tgatgcggct gtcgcattgc gctctaatga agaaattgag gtcatgttta
     1021 aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc
     1081 gaaggtgccg gaat
//
LOCUS       DAJSKJ010000335         1084 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000335 DAJSKJ010000000
VERSION     DAJSKJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.341"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..926
                     /gene="yeeO"
                     /locus_tag="ON345_003940"
     CDS             <1..926
                     /gene="yeeO"
                     /locus_tag="ON345_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR3073905.1"
                     /translation="NTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFVGAGLGLTI
                     SRYIGAVAILWVLAIGFNPALRISLTSYFKPLNFSIIWEVMGIGIPASVESVLFTSGR
                     LLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVGQIAQAEIQ
                     LRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFMPIWSASW
                     VLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMFAGWAVRAV
                     LFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
BASE COUNT          225 a          219 c          305 g          335 t
ORIGIN      
        1 ggaatacgaa aataccgcta ctgattaacg gtagcctgaa tattcttaat attattatta
       61 gcggcatatt gatttacggc cttttctcct ggccgggact gggatttgtc ggtgctgggt
      121 tgggtttaac catttctcgt tatattggcg cagttgcaat tttgtgggtg ctggcgattg
      181 gttttaatcc tgcgctaagg atttcgttaa cgagctattt taagccgctg aattttagca
      241 ttatctggga agtcatgggg attggtattc cagcgagtgt cgaatcagtg ttatttacca
      301 gtggtcggtt attaacccaa atgttcgttg ccgggatggg gaccagcgtt attgccggaa
      361 attttatcgc gttctcaatt gcggctctta tcaacttacc cggaagtgcg ctcggttctg
      421 cttctacgat cattacaggc cgaaggttgg gggtagggca gatagcgcaa gcagagattc
      481 agttgcggca tgtgttctgg ctgtccactc ttggattaac ggccatcgcc tggctaacgg
      541 ctccctttgc cggggttatg gcatcgtttt acacccagga tccacaggtt aaacacgtcg
      601 ttgtgattct gatttggcta aatgctttat ttatgcctat ttggtccgcc tcatgggtgc
      661 tacccgctgg atttaagggt gcccgtgatg cccgttacgc catgtgggtt tctatgttga
      721 gcatgtgggg ttgccgggtt gtagtcggtt atgtactggg aatcatgctt ggctggggtg
      781 tggttggagt ctggatggga atgtttgccg gctgggctgt gcgggccgtg ctgttttact
      841 ggcgaatggt tactggacgt tggctgtgga aataccctcg acccgaaccg caaaagtgtg
      901 aaaaaaagcc agctgtgtcg gaataaacga caaaatgcag attatttcag caaacgattt
      961 caaatttaaa aaacaggctt tgacattgtg ggtgggcatc gctaatattc gcctcgttct
     1021 cacgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt
     1081 tcga
//
LOCUS       DAJSKJ010000336         1077 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000336 DAJSKJ010000000
VERSION     DAJSKJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.342"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..771
                     /locus_tag="ON345_003941"
     CDS             <1..771
                     /locus_tag="ON345_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3073906.1"
                     /translation="QNSYIFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNP
                     AVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEV
                     DKNLTFRIRGLNNIHVLDCFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKD
                     PGAACTEQFNILGSFFTTDILSDYNHLDMGNGLLLKIFHNDGTATEFNRFSQFASFSS
                     SSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
     gene            complement(814..1014)
                     /gene="glgS"
                     /locus_tag="ON345_003942"
     CDS             complement(814..1014)
                     /gene="glgS"
                     /locus_tag="ON345_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR3073907.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          322 a          231 c          213 g          311 t
ORIGIN      
        1 cagaactcct atatttttgg cgtaacatac aatggagttg attatgattc gactgcccct
       61 aatgctatcg cagcaccaga atgcattgat ataaaaggag cgggtacctt taataatcac
      121 tataaaaacc ccgctgtctg tagtggaggt cctgagcctc aattatcagt aacctttcca
      181 gctcgcgtac aactctatat taaattagca aaaaatgcca atagagttaa taaaaacctg
      241 gtgttacctg atgaatatat cgctctagag tttaaaggta tgagtggcgc aggagctata
      301 gaagtagaca aaaatttgac cttcaggatt cgtggattaa ataatattca tgtccttgac
      361 tgctttgtta atgtcgcact ggaaccagct gatggcgttg ttgactttgg taaaataaat
      421 tcccgaacaa ttaaaaatac cagcgttagt gagacgttta gtgtagtcat gacaaaagat
      481 cctggtgcgg cctgtactga gcaattcaat attttaggga gctttttcac tacggatatt
      541 ttgagtgatt ataaccatct ggatatgggt aatggactgc tattgaagat atttcataac
      601 gatggaacag caacggaatt taaccgcttc tcacaatttg cttctttttc atcgtctagt
      661 gcaccttcgg tcaccgcacc attcagggca gaacttagtg cgaacccggc agagactgtt
      721 gttgagggac cgtttagtaa agacgtcatc ctcaaaatca cctataacta gcatctaata
      781 caaacactaa aacgggccat caggcccgtt atatcagtgc tctaactcca gctctcttgc
      841 ctgcatcatc tgttgacaat accaggcata acgtgcgcaa aaccaggttc tatgttcttc
      901 atccatgtta ccagtaacgg caagaatatc gaccgggcga ccttctgcgt gcattctggc
      961 aaaactacgc gccaggaaat cgaaattatt taaagaatta agactatgat ccataagcac
     1021 atcctccggt tatcgcatac cttttaaggc catatgcttt ttctgatttc agtattc
//
LOCUS       DAJSKJ010000337         1065 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000337 DAJSKJ010000000
VERSION     DAJSKJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.343"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>1065)
                     /gene="ydfJ"
                     /locus_tag="ON345_003943"
     CDS             complement(<1..>1065)
                     /gene="ydfJ"
                     /locus_tag="ON345_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR3073908.1"
                     /translation="TTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLA
                     EYAPKGKCGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVM
                     VFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLI
                     QTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAI
                     VLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAI
                     SKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSA
                     LEDAAEDQPRVVRAAQPSRSL"
BASE COUNT          314 a          272 c          232 g          247 t
ORIGIN      
        1 agacttctgg aaggttgcgc agctcttaca acacgcggtt gatcttccgc agcgtcttcc
       61 agcgcactta aatcacggtc tttcacctct ggcattttca gcgcagagat taaaccaatt
      121 actgaatatg ccatgatcat aatggcgatc ggataccagg attccgtcat ggtgcagaaa
      181 atacccgcca ggataggacc aaaaccggaa gcgataagac caccaatttc tttagaaata
      241 gccatccggg taaagcggtt tttacagccg aacatttctg ccatggtaat gttttccaga
      301 gcaaataatc ccagcaccgc acagttatga atcacaatca gtgcaaccat aatggtgctc
      361 ggggcatagc ttttatctac aatgatagaa agcattggcc atgccagcac aatcgcggag
      421 gtattcataa taatatacgg gatccggcga ccaattttat cggataacca accaaggaac
      481 ggaatggtca taaagccgag aatcgaacta atcatcaatg catctgttgg aattgctttg
      541 ttaaacaata aggtctgcac taaatagcct gcaaggaaag tctgaattaa cccggagtta
      601 cccgcctgac caaaacgcag ccctgttgcc agccagaagg atttgctctg gaacatgcta
      661 ccagcaggtg caggttttgc tgtgggttgg ttgctgtcat taaccttctc aaagaccggg
      721 ctttctttca gattcatacg taaccagata gcaaagacca tcacgacaac gctcgccagg
      781 aacggtatac gccatcccca cgccagcagt tcctctttac tgagaatgaa gaacataaag
      841 gcccagattg ccgttgcgct caaggttccg cagttagttc ccatagctac aaatgaggag
      901 ataattccgc acttaccttt tggtgcatat tccgccagca tcgtaccggc accggaaatt
      961 tccgcacctg cacccaaccc ctgaataata cgcagcgtca ccagcaagat gggggcaaaa
     1021 acaccaatct gtgcataggt cggtaacaca ccaattaagg tggta
//
LOCUS       DAJSKJ010000338         1034 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000338 DAJSKJ010000000
VERSION     DAJSKJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.344"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(90..308)
                     /gene="hha"
                     /locus_tag="ON345_003944"
     CDS             complement(90..308)
                     /gene="hha"
                     /locus_tag="ON345_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR3073909.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(334..708)
                     /gene="tomB"
                     /locus_tag="ON345_003945"
     CDS             complement(334..708)
                     /gene="tomB"
                     /locus_tag="ON345_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR3073910.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          307 a          205 c          206 g          316 t
ORIGIN      
        1 ataaagatca catagggtat gtcttcatgg tgaaaaagta taaaattctt aataaacagc
       61 cggttatagc tccgaaagcg aattatttat tagcgaataa atttccatac tgaggaaggg
      121 atcttgtcgt atagtttatt catggtcaat tcggcgaggc ggtgatctgc ggctgagtaa
      181 aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac acgctccagc
      241 gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggttttcgt taaaggtttt
      301 tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg caggattctc
      361 tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc atttctgaag
      421 atcctgcata ttaataccat aactactgaa caacataaag gtgtcatcca gatattcgtc
      481 gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa gtgcgaaggt
      541 cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg ggtcgttaac
      601 ccagccatga ttgctttctt caaggtttgc aaggcagtca tgatacaggg tttcacagag
      661 aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa cgcgtcccct
      721 tcttagcggt tgaactaacg gacacctttc gggatggaaa aaacttactg acctggactt
      781 gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat cgcattatta
      841 cgaaaaatta caattaggaa ggaatcggga caggactcac tttttcatac tagcactaac
      901 ccgtagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat tatcgccaga
      961 tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac cgaggaatga
     1021 ataaagaatt accg
//
LOCUS       DAJSKJ010000339         1029 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000339 DAJSKJ010000000
VERSION     DAJSKJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.345"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(1..418)
                     /locus_tag="ON345_003946"
                     /pseudo
     CDS             complement(1..418)
                     /locus_tag="ON345_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
     gene            complement(417..704)
                     /locus_tag="ON345_003947"
                     /pseudo
     CDS             complement(417..704)
                     /locus_tag="ON345_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          264 a          290 c          265 g          210 t
ORIGIN      
        1 ccggcggcag cgccccccac agccgcgcca gcgtatggcc gtccggctgt gctgccttac
       61 caccgcgcac cagccacatt gactcactgc ggctgtacac cgcctccccc ggcagcagcg
      121 gctcaaagtg aatgctccgc ccgccgttgt cgttgagtat cagtccgtca tcacgtagct
      181 gtaagcggat atcagaaggc gctttccagc cggggccgaa aacgcccacc ggtgcaggcg
      241 tcttcgtccg gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg
      301 caaggtccgt ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg
      361 aagtcatccc gcccggacac accgagcacg ccacgccggt gggcgcgcca attcttacac
      421 accttcatgc tgtacagcat ctggtttttg tcgttctccc ccagttcaaa ccaggtcacc
      481 acacctttca cccggcgctg tacgtcatcg ccctgccata ccgtcaggta ggccattttg
      541 tccagcacct gcgcaaattc aagggaaaga aactgctgac tgaccagaga aaggtcgagg
      601 gaaaaaagtg aagagagtga ctgggtgaga tgaaaggaaa ccaccgcaaa cgcgtccggt
      661 ggcaggccgt ccacttcaag tgtgaaacgt aatccggttg acatagcaac ctccgtgaaa
      721 atgaataata atccgcacat attgcatgta caacatgtcg ggcggatgag ataagcgagt
      781 cgacatctgg cgttaccggg gaatatcaga aaaatgtcat ggttatgtga tgatttagtc
      841 tgggatttta cttcagagaa acgggtgtct gtttagtcaa caaaataatc acaaacgcaa
      901 cggatgggtt ctcgacgtta ttcacggcag gctcgtacat tacagcgagt aataaaaaac
      961 ctgatatgca aagtcaaacc aatattcata atgtcttttt attcacaaca ataaataatt
     1021 caataacaa
//
LOCUS       DAJSKJ010000340         1016 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000340 DAJSKJ010000000
VERSION     DAJSKJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.346"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(34..924)
                     /gene="alsR"
                     /locus_tag="ON345_003948"
                     /gene_synonym="rpiR"
     CDS             complement(34..924)
                     /gene="alsR"
                     /locus_tag="ON345_003948"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HCR3073911.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT          273 a          253 c          219 g          271 t
ORIGIN      
        1 aggtaactcc gcgatggcgg aattaccttt ttcttatttc agcgcgcctg gtgagaagaa
       61 atcaacaatt gcgccggttt tttgcatatt aatattagct tgttcaatgt tgagctgggc
      121 gacagagaca aaaaaagcgt ccagcaaagt tagttgtaat attcttgccg aggcattacg
      181 acctaataac ggcgtttccg gggctggtga gcaaataata taatcggcca gtttcgctat
      241 tggtgaatgg tagctatgcg ttatacaaat aatctttgcc ccgttctttt ttgccagttc
      301 tacggccgct tttacatcac tggttcgccc ggaatgggtc actaccagca caacatctcc
      361 ttcctgtaac aacgaagcgg acatcatcat gatgtgagcg tccggatagg cctgacagcg
      421 tacgccaatg cgcaagaact tgtgctgtac atcagcacag atagcatttg atcctccggc
      481 accgtataaa tcccgctgtc tggcctgata gaaaaagcgg gcggcacggt ggatctcatc
      541 aacgttgacg atcgactgac cttccataat ggtgcgtaaa gtgatgttaa ataccttatt
      601 caccacatcc tgcggcgctt catcaaaagc caactcggaa ggcaatactt gttctgactg
      661 agaaaaataa tcttccagcg cactgcgtaa gttacgaaag ccgctaaacc ccagcagctt
      721 tgatacctta actatcatcg cttcagatac cgccagagct tctgcgacat ctttaattgc
      781 gggtgcacaa ctcaggttac cgggtttgag taaccactcc acgatgcggc tttcattttc
      841 tgtcattcct tcctgtttca ttcgcaggta aggcgctaac cctataccgt tcggaagcgc
      901 tgaatcaaac tctgactggc tcatcacgtt ctcttgtcct tgaattgaag acgccactat
      961 atcaccacag caggaacaga aaagatgctg gctacttcac aaaaaaataa aattta
//
LOCUS       DAJSKJ010000341         1014 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000341 DAJSKJ010000000
VERSION     DAJSKJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.347"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            12..980
                     /locus_tag="ON345_003949"
     CDS             12..980
                     /locus_tag="ON345_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR3073912.1"
                     /translation="MKDQITYPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETA
                     MYRMKQLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
BASE COUNT          257 a          265 c          280 g          212 t
ORIGIN      
        1 cttttgctga gttgaaggat cagatcacgt atcctcccga caacacagac cattccgtgg
       61 caaagcaaaa gttcagaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
      121 gctccctcac tttctggctg gatgatgagg cgattcaggc ctggtatgag tcggcaacgc
      181 cttcatcacg aggaaggccc cagcgctatt ctgatctcgc catcaccacc gttctggtga
      241 ttaaacgcgt attccggctg accctgcggg ctgcgcaggg ttttattgat tccatttttg
      301 ccctgatgaa cgttccgttg cgctgcccgg attacaccag tgtcagtaag cgggcaaagt
      361 cggttaatgt cagtttcaaa acgtccaccc ggggtgaaat cgcacacctg gtgattgatt
      421 ccaccgggct gaaggtcttt ggtgaaggcg aatggaaagt cagaaagcac ggcaaagagc
      481 gccgtcgtat ctggcgaaag ttgcatcttg ctgttgacag caacacacat gaagttgtct
      541 gtgcagacct gtcgctgaat aacgtcacgg actcagaagc cttcccgggc cttatccggc
      601 agactcacag aaaaatcagg gcagccgcgg cagacggggc ttacgatacc cggctctgtc
      661 acgatgaact gcgccgcaaa aaaatcagcg cgcttattcc tccccgaaaa ggagcaggtt
      721 actggcccgg tgagtacgca gaccgcaacc gtgccgttgc taatcagcgg ctgagcggaa
      781 gcaatgcacg gtggaaatgg acaacggaat ataaccgtcg ctcgatagcg gaaacggcaa
      841 tgtacagaat gaagcagttg ttgggagatt cactgacgct gcgtgactac gatggtcagg
      901 tagcggaagc tatggccatg gtgcgtgcgt tgaacaggat gacaaaggct gggatgccag
      961 aaagcgtgcg tattgcctga aaatccagcc agctacaggg tcgttcgcac gaaa
//
LOCUS       DAJSKJ010000342         1002 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000342 DAJSKJ010000000
VERSION     DAJSKJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.348"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>1002
                     /locus_tag="ON345_003950"
     CDS             <1..>1002
                     /locus_tag="ON345_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR3073913.1"
                     /translation="IPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNN
                     VSLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYS
                     KRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRL
                     NTYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLN
                     ISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLR
                     ASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMID"
BASE COUNT          283 a          216 c          209 g          294 t
ORIGIN      
        1 gtattcctta tctgacgcgt aaagggcaga ttcgctacac cactgctatg ggtaaaccga
       61 ccagcgttgg tggtgattcc ttacaacaac ccttcttctg gactggtgaa ttctcatggg
      121 gttggctgaa caatgtatcc ctgtatggtg gttcagtttt aacaaaccgt gattatcaat
      181 ctctggctgc cggcgttggc tttaacctta actcattggg ttcattatct tttgatgtca
      241 cacgatctga tgctcagttg cataatcagg ataaagaaac gggttatagc taccgcgcta
      301 actattcaaa acgttttgaa tctaccggta gccagctcac tttcgctggt taccgtttct
      361 ctgataaaaa ctttgtgaca atgaatgaat atatcaatga cactaaccat tacacgaatt
      421 atcagaatga aaaagagagt tatattgtca cgtttaacca gtatcttgaa tcattaaggt
      481 taaatacata cgtaagtttg gctcgtaata cttactggga cgccagcagt aatgtgaatt
      541 attcattatc acttagccgc gattttgata tcgggccatt aaaaaacgtc tccacttctc
      601 taacatttag ccgaataaac tgggaagaag acaaccagga tcaactgtac ctaaatattt
      661 cgattccctg gggaaccagt agaacattga gctatggtat gcaacgaaat caggataaca
      721 agatttcgca tactgcttcg tggtatgact cttccgatcg aaataattcc tggagcgttt
      781 ctgcttcagg cgacaatgat gaattcaaag atatgaaagc gtcactacgc gccagttatc
      841 agcataatac cgagaacggt cgtctctatc tctccgggac atcacagcga gacagttatt
      901 attctctgaa tgccagttgg aatggttcat tcacggcgac tcgtcacgga gccgctttcc
      961 atgactatag tggtagtgct gactcgcgtt ttatgatcga cg
//
LOCUS       DAJSKJ010000343          987 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000343 DAJSKJ010000000
VERSION     DAJSKJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.349"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..858
                     /locus_tag="ON345_003951"
     CDS             <1..858
                     /locus_tag="ON345_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR3073914.1"
                     /translation="LLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLF
                     IRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPV
                     IYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLI
                     CREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQINFTSYNILTI
                     AALVANSDMLAIIPSRFYNLFSRCWPLEKLSFPSLNEEQIDFSIHYNKFSLRDPILHG
                     VIDVIRNAF"
BASE COUNT          267 a          249 c          201 g          270 t
ORIGIN      
        1 cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc agcgaaagtg
       61 cttaatctga ccccctcggc aatcagtcag tctattcaga aactgcgcgt tatattccct
      121 gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc gatgcatcta
      181 catgagtata tcagtcaggg ccttgagtcc attcttggcg cgctggatat cgaaggaagc
      241 tatgataagc aacgaacgat aactattgcg accactccct cggtcggagc cctggtcctt
      301 cctgtcatct atcgggcgat taaaactcac tatccgcagc ttttactgcg caacccgccc
      361 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
      421 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
      481 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
      541 gcgcatgtac ttctgttacc ggaagagcaa aatttcagcg gtctgcggca gagagttcaa
      601 gaaatgtttc cggaccggca aatcaatttc accagctaca acattttgac aatcgctgca
      661 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cctgtttagc
      721 cgctgctggc cgctggaaaa actgtctttt ccgtccttaa atgaggagca aatagatttc
      781 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggagt aatagatgtt
      841 atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag aacgataaaa
      901 tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt tcaggtccgt
      961 ttcaatgata tactgccagt cgttaat
//
LOCUS       DAJSKJ010000344          978 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000344 DAJSKJ010000000
VERSION     DAJSKJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.350"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..682)
                     /locus_tag="ON345_003952"
     CDS             complement(<1..682)
                     /locus_tag="ON345_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HCR3073915.1"
                     /translation="MMKSKLKLMPLLVSVSLMSGCTILPGSNMSTYGKDVIKQQDADY
                     DIGRMVNVYPLTPRLIEQLRPRPNVAQPNMSLDQELSNYQYRVGSGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTIFYPYIGKVQVKGRTLAEIRQTITQRLAAYITDPQ
                     VDVNVAAFRSQKVYVSGQVNKSGQQAITNVPLTVLDAINAAGGLSELADWRNVVLTHD
                     GKEQRISVQ"
BASE COUNT          235 a          252 c          230 g          261 t
ORIGIN      
        1 cctgcactga aatgcgctgt tctttgccat cgtgagtcag taccacgttg cgccagtcgg
       61 ccagttcgct cagaccaccc gccgcgttga tcgcgtccag taccgtcagc ggtacgttgg
      121 tgatagcctg ctggccggat ttgttcacct gcccggagac atacactttt tgcgaacgga
      181 aggcggcaac gttcacgtca acctgcgggt cggtgatgta ggcggcgaga cgctgggtaa
      241 tggtctggcg aatttccgcc agcgtgcgcc ctttcacctg cactttgcca atatagggat
      301 agaaaatggt gccgtccggc tggacccagt taccggtatc gctggagcta cggtactgcc
      361 ccgccggggt ggtcaactcc gggtgatccc acaccgtgac gttcagcacg tcgcctgaac
      421 cgacgcgata ctggtaattg cttaactcct gatccagcga catgttcggt tgcgcgacgt
      481 tgggacgtgg gcgtaattgt tcaatcagtc gcggggtcag cggataaaca ttcaccatcc
      541 ggccaatgtc ataatcagca tcctgctgct taatcacatc ttttccatag gtagacatat
      601 tgctgcctgg aagtatggtg caaccactca tcaagcttac cgacaccaat aatggcatca
      661 attttagttt ggatttcatc attgattatt tatcactttg gcagaaaatt atcctgtgcg
      721 aattatatta atccgtacga tttgattacc tgtaaactca ccatgcattt atttgccgat
      781 gaatataact ggcatatatc ctaaaaaagt tgggattcat ccgcaggcta ttgacagaat
      841 aattcaggct ttacgtcacg ccttcaggca tgctatcgcc cttggctttc agctaccaat
      901 tcacctaaaa atcatgttgt tataggaaac ccacccattc ataaaaagaa tagagaatat
      961 tcctggatat ataattgc
//
LOCUS       DAJSKJ010000345          977 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000345 DAJSKJ010000000
VERSION     DAJSKJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.351"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(28..951)
                     /locus_tag="ON345_003953"
     CDS             complement(28..951)
                     /locus_tag="ON345_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR3073916.1"
                     /translation="MAKQKFKITNWPTYNKALINRGSITFWLDDEAIQAWYESATPSS
                     RGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLRCPDYSCVSRRAKS
                     VNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSKTHEI
                     ICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHDELRRKKISALIPPRK
                     GAGYWPGEYADRNRAVANQRLTGSNAWWKWTTDYNRRSIAETAMYRVKQLFGGSLTLR
                     DYDGQVAEAMALVRALNKMTKAGMPESVRIA"
BASE COUNT          202 a          263 c          267 g          245 t
ORIGIN      
        1 aagtctcccc cgtagcgggt tgtgttttca ggcaatacgc acgctttcag gcatacctgc
       61 tttcgtcatt ttgttcagcg ctcgtaccag ggccatagcc tccgcaacct gaccatcgta
      121 gtcacgcagc gtcagtgaac ccccgaacag ctgttttacc cggtacatcg ccgtttccgc
      181 tatcgagcga cggttgtaat ctgttgtcca tttccaccac gcattactcc cggtcagccg
      241 ctgattagcc actgcacggt tacggtctgc atattcaccg ggccagtaac ccgcaccttt
      301 tcggggtggg ataagcgcgc tgattttctt acgccgcagt tcatcgtgac agagccgggt
      361 gtcgtaagcg ccatcggcgg cggctgacct gattttccgg tgggtttgcc ggattaaccc
      421 ggggaaggcc tctgagtccg taacgttgtt cagcgacagg tctgcacaga tgatttcatg
      481 tgttttactg tcaacggcga gatgcagctt acgccagata cggcggcgtt cctggccatg
      541 ctttttgact ttccactcgc cttcaccgaa gaccttcagc ccggtggaat caatcaccag
      601 atgtgcgatt tcaccccggg tgggcgtttt gaaactgaca ttaaccgact ttgcccgcct
      661 gctgacacag ctgtaatccg ggcagcgtag cggaacgttc atcagagaaa aaatggaatc
      721 aataaagccc tgcgcagcgc gcagggtcag cctgaatacg cgtttaatga ccagaacggt
      781 ggtgatggca aggtcagaat agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc
      841 ataccaggcc tgaatagctt catcatccag ccagaaagtt atggagccac ggttgatgag
      901 ggctttattg taggtgggcc agttggtgat tttgaacttt tgctttgcca cggaacggtc
      961 tgcgttgtcg ggaagat
//
LOCUS       DAJSKJ010000346          967 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000346 DAJSKJ010000000
VERSION     DAJSKJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.352"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>967
                     /locus_tag="ON345_003954"
     CDS             <1..>967
                     /locus_tag="ON345_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR3073917.1"
                     /translation="SVLIIGCASAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKY
                     SLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEEAQQNLIDGCYP
                     IEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHY
                     APHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIP
                     AINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTV
                     SQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQV"
BASE COUNT          296 a          207 c          192 g          272 t
ORIGIN      
        1 tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc
       61 gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc
      121 gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat ccatctggac
      181 ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca
      241 ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt
      301 taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta
      361 tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa
      421 ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat
      481 gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat
      541 cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa
      601 agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt
      661 aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat
      721 ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac
      781 gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac
      841 cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat
      901 acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg
      961 caagttg
//
LOCUS       DAJSKJ010000347          965 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000347 DAJSKJ010000000
VERSION     DAJSKJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.353"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..268
                     /locus_tag="ON345_003955"
     CDS             <1..268
                     /locus_tag="ON345_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073918.1"
                     /translation="LYIQPILGMFLIGIGLWGFYAPDEGTCTNLNIELCAVSAIDVLK
                     PAIFVFLGIITVAGWYFTELQPKLRGEGKYMKVKTGVKPEDFVK"
     gene            304..588
                     /locus_tag="ON345_003956"
     CDS             304..588
                     /locus_tag="ON345_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073919.1"
                     /translation="MNKSWLEQQVQNEKLKKVQEEKKDGTIQKGLYRVRIKDKYRWDL
                     WENDFRVVIAYSKDDAELKFTEMTGFNEHYYIESIEKIVLSDIEKILLEN"
     gene            complement(609..>965)
                     /locus_tag="ON345_003957"
     CDS             complement(609..>965)
                     /locus_tag="ON345_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="HCR3073920.1"
                     /translation="VMKYQVKEFINEKYSKAVNILKDNLKEHYHIFYGLRLSEILFPA
                     SEYGSEMFFQEFEAINSVILPLVIFDLIDRKPIMVIGFGEVCGVDSLVDSGIEVVSLD
                     GLSDLLLVEKLTPLFN"
BASE COUNT          348 a          156 c          188 g          273 t
ORIGIN      
        1 gttatacatc caaccgatat tggggatgtt ccttataggt ataggcttat gggggttcta
       61 tgcacctgat gagggaactt gtactaatct caatattgag ttatgcgctg tatcagctat
      121 tgatgttctg aagcctgcta tttttgtatt tttaggcatt attaccgttg ccggatggta
      181 ctttaccgaa cttcagccga agctaagggg ggagggcaag tatatgaagg taaaaacagg
      241 tgtaaaaccg gaagatttcg ttaagtaaaa accgagccta aagacgttaa ccgtggggga
      301 tttatgaata aatcatggct tgaacaacag gtgcaaaatg aaaaattaaa aaaagtacag
      361 gaagagaaaa aagatggcac tatccaaaaa ggattgtaca gggttcgtat aaaagacaaa
      421 tacaggtggg atttgtggga aaatgatttc agagttgtta ttgcttattc gaaagatgat
      481 gcagagctaa aatttacaga aatgacaggt tttaatgagc attactatat agaaagcatt
      541 gaaaaaatag tgctttcaga tatagaaaaa atcttactgg aaaactgaaa agatagttat
      601 ccagaggttt agttaaataa tggcgtcagt ttttcaacca gtagcagatc gcttaaaccg
      661 tctaaagaaa caacttctat accagaatca acaagtgaat caactccaca tacttcaccg
      721 aagccgataa ccataatcgg ctttcggtct atcaaatcaa aaatcaccaa aggcaatatt
      781 acactgttaa tcgcttcaaa ctcctgaaaa aacatttcag agccgtattc gctggcagga
      841 aaaagaatct cacttaatct caaaccataa aaaatatggt agtgttcttt aaggttatcc
      901 tttaaaatat taacagcctt agaatatttt tcgttaataa actctttaac ctgatatttc
      961 ataac
//
LOCUS       DAJSKJ010000348          961 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000348 DAJSKJ010000000
VERSION     DAJSKJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.354"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>961
                     /locus_tag="ON345_003958"
     rRNA            <1..>961
                     /locus_tag="ON345_003958"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          238 a          218 c          310 g          195 t
ORIGIN      
        1 ataactactg gaaacggtag ctaataccgc ataatgtcgc aagaccaaag agggggacct
       61 tcgggcctct tgccatcgga tgtgcccaga tgggattagc ttgttggtgg ggtaacggct
      121 caccaaggcg acgatcccta gctggtctga gaggatgacc agccacactg gaactgagac
      181 acggtccaga ctcctacggg aggcagcagt ggggaatatt gcacaatggg cgcaagcctg
      241 atgcagccat gccgcgtgta tgaagaaggc cttcgggttg taaagtactt tcagcgggga
      301 ggaagggagt aaagttaata cctttgctca ttgacgttac ccgcagaaga agcaccggct
      361 aactccgtgc cagcagccgc ggtaatacgg agggtgcaag cgttaatcgg aattactggg
      421 cgtaaagcgc acgcaggcgg tttgttaagt cagatgtgaa atccccgggc tcaacctggg
      481 aactgcatct gatactggca agcttgagtc tcgtagaggg gggtagaatt ccaggtgtag
      541 cggtgaaatg cgtagagatc tggaggaata ccggtggcga aggcggcccc ctggacgaag
      601 actgacgctc aggtgcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac
      661 gccgtaaacg atgtcgactt ggaggttgtg cccttgaggc gtggcttccg gagctaacgc
      721 gttaagtcga ccgcctgggg agtacggccg caaggttaaa actcaaatga attgacgggg
      781 gcccgcacaa gcggtggagc atgtggttta attcgatgca acgcgaagaa ccttacctgg
      841 tcttgacatc cacggaagtt ttcagagatg agaatgtgcc ttcgggaacc gtgagacagg
      901 tgctgcatgg ctgtcgtcag ctcgtgttgt gaaatgttgg gttaagtccc gcaacgagcg
      961 c
//
LOCUS       DAJSKJ010000349          952 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000349 DAJSKJ010000000
VERSION     DAJSKJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.355"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            58..153
                     /locus_tag="ON345_003959"
     CDS             58..153
                     /locus_tag="ON345_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073921.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            317..>952
                     /locus_tag="ON345_003960"
     CDS             317..>952
                     /locus_tag="ON345_003960"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HCR3073922.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTD"
BASE COUNT          233 a          234 c          244 g          241 t
ORIGIN      
        1 agcaataaca gcttgagtta tctcaacaca aaataataac cgttaagggt gtagcctatg
       61 atcaacacaa atatgaaata ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc
      121 tgggccgaac tcctctggat cattactcac tgatccttga ccccgctgcg gcggggttgt
      181 catttgcttt gccacaaggt ttctcctctt ttatcaattt gggttgttat caaatcgtta
      241 cgcgatgttt gtgttatctt taatattcac cctgaagaga atcagggctt cgcaaccctg
      301 tcattaagga ggagctatgt cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc
      361 caaagataaa acttaccact actacagcct gccgcttgct gctaaatcac tgggcgatat
      421 cacccgtcta cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg
      481 taactcggtt accgaagagg atatccacgc gctggcagga tggctgaaaa atgcccatgc
      541 tgaccgtgaa attgcctacc gcccggcaag ggtgctgatg caggacttta ccggcgtacc
      601 tgccgttgtt gatctggcgg caatgcgcga agcggttaaa cgcctcggcg gcgatactgc
      661 aaaggttaac ccgctctcac cggtcgacct ggtcattgac cactcggtga ccgtcgatcg
      721 ttttggtgat gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga
      781 acgttatgtg ttcctgaaat ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc
      841 aggcacaggc atttgccatc aggttaacct cgaatatctc ggcaaagcag tgtggagtga
      901 attgcaggac ggtgaatgga ttgcttatcc ggatacactc gttggtactg ac
//
LOCUS       DAJSKJ010000350          938 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000350 DAJSKJ010000000
VERSION     DAJSKJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.356"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ON345_003961"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ON345_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR3073923.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          249 a          231 c          207 g          251 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccag tagttcatcc agtgcttcaa tatcacgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc
      421 atgttcagct ttggtgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcagatcagt cagtggtagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg
      601 cgactaatga cagccaacaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcagatt taaagaaa
//
LOCUS       DAJSKJ010000351          936 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000351 DAJSKJ010000000
VERSION     DAJSKJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.357"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..759)
                     /gene="bglG"
                     /locus_tag="ON345_003962"
     CDS             complement(<1..759)
                     /gene="bglG"
                     /locus_tag="ON345_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR3073924.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGL"
BASE COUNT          261 a          214 c          176 g          285 t
ORIGIN      
        1 caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt aattttgttt
       61 tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa gaatacgcca
      121 ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt cctggtaatt
      181 aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg tgacacctgc
      241 aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa taaagcccac
      301 ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg cttcttcccc
      361 tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacgggt tgggcagcaa
      421 cacgttttgc tgaaagcgtt taatcgcaaa ctggcaatgg tcagttagcg agatataaat
      481 actgtcctgt aattttccca agcgctcctg cgctaaagag ataatacgat cacaggttgc
      541 catcacctca agaggaatat gacttaagag ttcgcttaat cgcccgttca gttcatgact
      601 gctcaaggca tactcttttt ctattccact tgagttaatt ctttcgccag cgcgtttttg
      661 aaagccaatt ccgcgcccca tgacgacttt ttcccgctgt tgatcatcaa taaccaccac
      721 aacattattg ttgagaattt tggtgatttg catgttcata gcaaggacct tttttataaa
      781 caaaaaaaac cgacttcacc agtattctct ggttatgtca ggttttgcct gcgaatgcag
      841 taacaatcca gtcatttatt aatggttttt ataacgaaca tctaggttcg gaaattaatt
      901 taattgcgtg cagagaaaat agcaatgcgc tattga
//
LOCUS       DAJSKJ010000352          931 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000352 DAJSKJ010000000
VERSION     DAJSKJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.358"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..808
                     /gene="gadX"
                     /locus_tag="ON345_003963"
     CDS             <1..808
                     /gene="gadX"
                     /locus_tag="ON345_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR3073925.1"
                     /translation="NCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVEN
                     FVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRY
                     AEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIAS
                     ELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYF
                     IYVFRNYYGMTLTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          269 a          177 c          211 g          274 t
ORIGIN      
        1 gaattgtcta attgcgtacg caagacataa atatattctc accatggtta atggtgaata
       61 tcgctatttt aatggcggtg acctggtttt tgcggatgca agccaaattc gagtagataa
      121 gtgtgttgaa aattttgtat tagtatcaag ggatacgctt tcattatttc tgccgatgct
      181 caaggaggag gcattaaatc ttcatgcaca taaaaaaatt tcttcattac tcgttcatca
      241 ctgtagcaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga ataagaatct
      301 tcgctatgca gaaatgctac gtaaaagagc attaatcttt gcgttgttat ctgtttttct
      361 tgaggatgag cactttatac cgctgctttt gaacgttttg caaccgaaca tgcgaacaag
      421 agtttgtacg gttatcaata ataatatcgc ccatgagtgg acactagccc gaatcgccag
      481 cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag agacatcata
      541 ttcacagttg cttactgagt gtagaatgca acgtgctttg caacttattg ttatacatgg
      601 cttttcaatt aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt atttcattta
      661 cgtctttcga aattattatg ggatgacgct cacagagtat caggagcgat cggcgcaggg
      721 attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt acggcactga
      781 ccgttctgcg gaaggaataa gattatagaa ttttactcag acataaaaaa aacccggcat
      841 aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta taacactccc
      901 tgttggcacg ggaaactttg tgctctcagt a
//
LOCUS       DAJSKJ010000353          931 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000353 DAJSKJ010000000
VERSION     DAJSKJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.359"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..153
                     /locus_tag="ON345_003964"
     CDS             <1..153
                     /locus_tag="ON345_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis FlhA domain protein"
                     /protein_id="HCR3073926.1"
                     /translation="GAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRHIRMT
                     ATIGGK"
     gene            153..545
                     /gene="flhe"
                     /locus_tag="ON345_003965"
     CDS             153..545
                     /gene="flhe"
                     /locus_tag="ON345_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR3073927.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(666..>931)
                     /locus_tag="ON345_003966"
     CDS             complement(666..>931)
                     /locus_tag="ON345_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR3073928.1"
                     /translation="EQVPRTGVKKPDTEQLNLLKKSQRAWLDFRDKECELILSNEDVQ
                     DLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLTRG"
BASE COUNT          225 a          198 c          246 g          262 t
ORIGIN      
        1 ggtgcgccgc cagtattgtt ggtgaaccac gcgctgcgac cattattgtc tcgctttctg
       61 cgccgcagct tgccgcagtt agtggtgctg tcgaatctgg aactatcaga taaccgacat
      121 atccgtatga cggcgacgat tggcggcaaa taatgagagc cttattagca atattattgt
      181 ttccgctgct agtgcaagcc gccggggagg ggatgtggca ggcaagtagt gtcggaatta
      241 cgctaaatca tcgcggtgag tcgatgtcgt cagcgcctct ttctactcga caacctgctt
      301 caggattgat gacgctggtg gcgtggcggt atcagcttat cggcccgaca cctgcaggac
      361 tgcgggttcg cttgtgttcg caatctcgtt gtgtcgaatt agatgggcag agcggaacca
      421 ccgtggcctt ttccggtata gcggcagcag aaccgttgcg ctttatctgg gaagtgcctg
      481 gcggtgggcg gttaattccg tcgctgaaag tacaacgtaa tgaagtgatt gtgaattatc
      541 gctgaaggac aaaaaaaatt acgcgctcgc gagtatgttt aataactcag tgaaaaaggc
      601 tggatgtttt gttgcaatca tttatcagga atagcttgca acaaaacatg ccaaaaagat
      661 gtgaattatc ctcttgtcaa agggcttgga taaaaatctt cagagttacg gtatagctgc
      721 aactggcgag ttcgcgtatt ggtctgtatg atcatacatg agagccattc tgattcagaa
      781 taagggtcac ttaaatcctg aacgtcctca tttgaaagga ttaattcaca ttctttgtcc
      841 ctaaaatcca gccaggctcg ctgtgatttt ttaagcaagt taagttgttc ggtatcaggt
      901 ttttttacac ctgtgcgtgg cacttgttct a
//
LOCUS       DAJSKJ010000354          918 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000354 DAJSKJ010000000
VERSION     DAJSKJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.360"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            81..812
                     /gene="artJ"
                     /locus_tag="ON345_003967"
     CDS             81..812
                     /gene="artJ"
                     /locus_tag="ON345_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR3073929.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVCPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT          271 a          210 c          206 g          231 t
ORIGIN      
        1 ataaattcac ttgatggcat tgttatccca tgccgcagac acggccaaaa atcataagat
       61 tgacagacgg gagttccatc atgaaaaagt tagttcttgc cgctttactt gcttccttta
      121 ctttcggtgc ttctgccgca gagaaaatca attttggcgt ttcagccacc tatccaccct
      181 ttgaatctat aggtgctaat aatgagattg tcggctttga tatcgatctg gcaaaagcct
      241 tgtgcaaaca aatgcaggca gaatgtactt ttactaatca cgcgttcgac agcctgatcc
      301 cgtcactgaa attcagaaaa tatgacgccg taatctccgg tatggatatc acgcccgagc
      361 gtagcaagca ggtatcgttt accacgccct actatgaaaa ctcagccgtc gtgattgcca
      421 aaaaagatac ctacaaaacg tttgccgatc tgaaaggcaa acgtattggg atggaaaacg
      481 gcaccacgca ccagaaatat attcaggatc agcaccccga agtgaagact gtctcctatg
      541 acagttatca gaatgccttt atcgatctga aaaatggtcg tattgatggg gtatttggtg
      601 acacagcggt ggtaaacgaa tggctgaaaa ccaatccgca actgggcgtt gctactgaaa
      661 aagtgaccga tccgcaatac ttcggcaccg gtctgggcat cgctgtatgt ccggataaca
      721 aagctctgct ggaaaaactg aataacgcgc tggcagcaat taaagctgac ggtacatatc
      781 agaaaatcaa tgaccagtgg tttccgcagt aattgagact gatgacaaaa gtaagattgc
      841 ctgatgcgct gcgcttatcc ggcccacatc aactctgcaa tgtattgatt tagcatgatt
      901 ttgtaggccg gataaggc
//
LOCUS       DAJSKJ010000355          903 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000355 DAJSKJ010000000
VERSION     DAJSKJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.361"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            30..71
                     /locus_tag="ON345_003968"
     CDS             30..71
                     /locus_tag="ON345_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073930.1"
                     /translation="MVQLVDLARCVSF"
     gene            109..>903
                     /gene="dosC"
                     /locus_tag="ON345_003969"
     CDS             109..>903
                     /gene="dosC"
                     /locus_tag="ON345_003969"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR3073931.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSKEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLR"
BASE COUNT          251 a          179 c          214 g          259 t
ORIGIN      
        1 ttcagtatat cttcatattt caggagaata tggtgcagtt agtcgatctc gcacgttgcg
       61 tttcgttttg attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat
      121 tttaaaagaa tgaaagatga gtggaccgga cttgtcgaac aggcagatcc tctcattcgt
      181 gctaaagccg cagaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga
      241 attgtccgca tcgacccgca tgccgaagaa ttcttgagta aggaacaagt tgagcggcag
      301 ttgaaaagtg cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc
      361 gaaaggctaa tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg
      421 gtagaaattg tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc
      481 tcttcggatt attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt
      541 gatatcgcga tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag
      601 gaagatgaaa actatcgtat cttctcgttg ctggaaaacg ccgaagaaga aaaagaacgg
      661 caaatagcct caatactttc atgggaaata gatattatct ataaaatcct gctggattct
      721 gatttaggca gtagtttgcc tttaagccag gctgattttg gcctgtggtt taaccataaa
      781 ggtcgacatt attttagtgg cattgccgaa gtaggccata tctcccgtct gattcaggat
      841 ttcgacggta tattcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg
      901 cgg
//
LOCUS       DAJSKJ010000356          901 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000356 DAJSKJ010000000
VERSION     DAJSKJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.362"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            651..>901
                     /locus_tag="ON345_003970"
     CDS             651..>901
                     /locus_tag="ON345_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073932.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRV"
BASE COUNT          230 a          229 c          270 g          172 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccacgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacgctt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 t
//
LOCUS       DAJSKJ010000357          892 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000357 DAJSKJ010000000
VERSION     DAJSKJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.363"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..328)
                     /locus_tag="ON345_003971"
     CDS             complement(<1..328)
                     /locus_tag="ON345_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR3073933.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKT"
     gene            485..661
                     /locus_tag="ON345_003972"
     CDS             485..661
                     /locus_tag="ON345_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR3073934.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWGFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
BASE COUNT          279 a          160 c          172 g          281 t
ORIGIN      
        1 gtgttttata ttgtccaaaa aaggaaagca tttttgctag ttctttcatt tctgcaaata
       61 aaatatcagt gcattcacgt tgtgatattt ttgttccgga acttgaggca ctaaaagcaa
      121 cattaataga acctgggcca gggaaatggt atttaataag tctgtaagga tcaacttttg
      181 ctgagatatc gcttaggccg agtgagagaa agtcttgttc taccatatca ccatattgca
      241 tatcatcagc agaaaaatca tcgaataaac taatagtttc gaaaagtgta cagggaatat
      301 taattggcgg aagtaatatg ttattcattt gcatttgcct tggttatatt taagctcaat
      361 atcaattcga gaacaaatgc tagggtaaaa aaatatttaa aaaagaagta cacttcacat
      421 tttttcatta catctctgaa gagaacctcg ttaatgattg gtcttgctca ttgttaataa
      481 caaaatgttg aatttaccga ttaggaaact ttttcctaca cggattgcct ccagctatgg
      541 catgaacttt gagtttatgc cagaactgaa gtggggcttc ggctaccctg gcgcgattat
      601 ctttatgatc ctcgcgggcc tgacaccgta tctgtacttt aagcgtaaga actggctgta
      661 actatacgat gtcgctgtga gtaagttagt gtgctgacag cgacatttta gccgagggaa
      721 tcgcagataa cataattgtt gtgaataata tccatgccac gttgagttgg acgcttaatg
      781 actcacatct caccccttca attgctgcag agataataga aaaaagtgtc aaacccaatg
      841 aaatcaatac aaagtagatt gccttacatc cagtatctct tattcttttt gc
//
LOCUS       DAJSKJ010000358          889 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000358 DAJSKJ010000000
VERSION     DAJSKJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.364"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            107..631
                     /gene="aroL"
                     /locus_tag="ON345_003973"
     CDS             107..631
                     /gene="aroL"
                     /locus_tag="ON345_003973"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR3073935.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            681..872
                     /gene="yaiA"
                     /locus_tag="ON345_003974"
     CDS             681..872
                     /gene="yaiA"
                     /locus_tag="ON345_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR3073936.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          246 a          213 c          230 g          200 t
ORIGIN      
        1 gtggaatttt ttctttacaa tcgaaattgt actagtttga tggtatgatc gctattctca
       61 tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa cccacgatga cacaacctct
      121 ttttctgatc gggcctcggg gctgtggtaa aacaacggtc ggaatggccc ttgccgattc
      181 gcttaaccgt cggtttgtcg ataccgatca gtggttgcaa tcacagctca atatgacggt
      241 cgcggagatc gtcgaaaggg aagagtgggc gggatttcgc gccagagaaa cggcggcgct
      301 ggaagcggta actgcgccat ccaccgttat cgctacaggc ggcggcatta ttctgacgga
      361 atttaatcgt cacttcatgc aaaataacgg gatcgtggtt tatttgtgtg cgccagtatc
      421 agtcctggtt aaccgactgc aagctgcacc ggaagaagat ttacggccaa ccttaacggg
      481 aaaaccgctg agcgaagaag ttcaggaagt gctggaagaa cgcgatgcgc tatatcgcga
      541 agttgcgcat attatcatcg acgcaacaaa cgaacccagc caggtgattt ctgaaattcg
      601 tagcgccctg gcacagacga tcaattgttg attttagagc gcctatactt aacgttcatc
      661 ccgtgaaata aggaagaacg atgccaacga aaccgcctta tcctcgtgaa gcatatatag
      721 tgacgattga aaaaggaaag ccaggacaga cggtaacctg gtaccaactc agagccgatc
      781 atcctaaacc agactcgttg atcagtgaac atccgaccgc tcaggaagcg atggatgcga
      841 aaaaacgcta tgaggaccct gacaaagagt gaccgcatca gactgctcg
//
LOCUS       DAJSKJ010000359          888 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000359 DAJSKJ010000000
VERSION     DAJSKJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.365"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            111..>888
                     /locus_tag="ON345_003975"
     CDS             111..>888
                     /locus_tag="ON345_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073937.1"
                     /translation="MTTQKEMEEIRAALSWFDVRRYDGLKDLTLEQIYAELERRMLAY
                     KTRQQWETAGKDNQMQAIYHDATIRCGDVILKSDWISDNHRLSHSYAVRPMTRVQLFN
                     YGRAMYRLETSEQTDPVAVSSDYISEYLKQGGMNPANKMLIEIDLEEASSDDLAEHLK
                     VLIALWQKHLKTPKPPKRKFRFGHKTFERILDYKVIPLMDLISWEQLYNEGKNIPFTI
                     LADILHGNNGVRSSENIKDTDYDYAKSYLDNDEYFKVLNDF"
BASE COUNT          297 a          178 c          190 g          223 t
ORIGIN      
        1 aggtcactta ctgtgctcac accattttga aaacccgctc ctcttttcca ctttctctat
       61 catgttgaat gggctaacgt ttaggaaaca caaaagcaat cggtagaaca atgactacac
      121 aaaaggaaat ggaagaaatc agggcagcgt tatcttggtt tgatgtacgc cgatatgatg
      181 gcctgaaaga cctcacctta gaacaaatct atgcagagct tgaaagacgg atgttggctt
      241 acaaaacaag acaacaatgg gaaacagcag gcaaagataa tcaaatgcaa gcgatatacc
      301 atgatgccac aatccgatgt ggtgatgtga tacttaaatc agactggatt tcagacaatc
      361 acagactgtc gcatagctat gctgtaagac caatgaccag agttcaattg ttcaactatg
      421 ggcgggctat gtatcggctg gagacttcag agcaaacaga ccctgttgca gtgagttccg
      481 actacatctc tgagtatctg aaacaaggag gcatgaaccc tgctaacaag atgctgattg
      541 aaattgactt ggaagaagcc agcagtgacg atctcgcaga acatctgaaa gtactgattg
      601 ctctatggca aaagcacctg aaaacgccta aacccccgaa acgaaaattt aggtttggtc
      661 ataagacctt tgaaagaata cttgattaca aagtcatccc tttgatggat ttgatctcat
      721 gggaacagct ctacaacgaa ggtaagaaca tacctttcac cattttagct gacatcctgc
      781 atggtaataa tggggtcaga agtagtgaga acatcaaaga tactgattat gattacgcga
      841 aaagttatct tgataacgat gagtacttca aggttttaaa tgatttct
//
LOCUS       DAJSKJ010000360          877 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000360 DAJSKJ010000000
VERSION     DAJSKJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.366"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..140
                     /locus_tag="ON345_003976"
     CDS             <1..140
                     /locus_tag="ON345_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR3073938.1"
                     /translation="VDIVVRVASNDVLTILRAVDKESAQEFVQETLQEVWETEDDWFI
                     Q"
     gene            complement(427..>877)
                     /locus_tag="ON345_003977"
     CDS             complement(427..>877)
                     /locus_tag="ON345_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073939.1"
                     /translation="NRYLEYGLQQMLEGYSVILAREFFMPENRKHTPEHDESWVIICD
                     ALLGRLMRCMFQGRRYLQLDAEEMTGRLDAYRKIRNGDWVQNTYARPLTMSEMVVMFG
                     YVYRESKPCHLAREMGINTKTVNTFLYLGLGKNGLRYRSVKHLVGRA"
BASE COUNT          229 a          246 c          173 g          229 t
ORIGIN      
        1 atgtggacat tgttgtcaga gttgccagca atgacgttct caccatttta agagccgtcg
       61 ataaagagtc tgcgcaggag tttgtacagg aaacactaca ggaagtctgg gaaacagagg
      121 atgactggtt tattcaataa tgagaatata atcactgtac tcagtcatct tatttgtcag
      181 aattacagtt gacgtaaaaa cctttacata tggttagcta cattcctgtt tcggggagct
      241 gttcagctcc ccatttttta ttgcatcatt acggcagtca ctgcacagag cgttaccact
      301 gcacccagcc acacaaaaag catgaaacac atcgctcctg ctgtgtagcg cttctttacg
      361 tatttcagat ttgcctgttt catgacaatt tacctgattg ctcttcagta ccggataaca
      421 atacggctac gcccgtccca ccaggtgctt cacactcctg tacctcagac catttttacc
      481 aagccccaga tacagaaagg tgttcaccgt cttcgtgttt atccccattt cacgcgcaag
      541 atgacacggc tttgactcac ggtacacata accaaacatc accaccatct cggacatcgt
      601 cagaggacgc gcatacgtgt tctgcaccca gtcaccgtta cggattttcc tgtatgcatc
      661 cagacgcccc gtcatctcct ccgcatccag ctgaagataa cggcgtccct gaaacataca
      721 gcgcatcagc ctgccaagca gcgcatcaca gataatcacc catgattcat catgctccgg
      781 tgtgtgcttc ctgttctccg gcataaaaaa ctcccgggcc agtatgacac tgtacccctc
      841 aagcatctgt tgcaggccat actccagata acggttg
//
LOCUS       DAJSKJ010000361          849 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000361 DAJSKJ010000000
VERSION     DAJSKJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.367"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(108..>849)
                     /locus_tag="ON345_003978"
     CDS             complement(108..>849)
                     /locus_tag="ON345_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3073940.1"
                     /translation="KLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLETYQSN
                     FQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEM
                     SKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVA
                     IEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAK
                     QGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          260 a          176 c          139 g          274 t
ORIGIN      
        1 aacgtagaac aaattgaacc aaatttatta ggcgtttacg ccggcttatt attagcgatg
       61 aaaaacaagt aaaaaataaa aaaggctgca taatgcggcc tttttattta ttcaaaccaa
      121 ttatgaataa cctcttcgaa tctttgagtt acgccatccc aactgtagtg ttcaaacaca
      181 aagtcctgac cttgctttgc aactgctgtt aattcaggat tcgctaacgt ttttaaaata
      241 tcgctgctta tggagtctgc agtcataggt tcttttaaat ggaatcccgt agtattttct
      301 ttgacaaatt ccgtcatccc cccacgcgta ctcactaaaa caggcttacc agcacccata
      361 gcttcaattg ccaccatgca aaatggttcc tggaactggg aaggaatgac gactaaatct
      421 gccagtgggt aataacaatg catcttttca ggcggtatgc ttcctaacat aatgcaacga
      481 tccttcaaac gttttgcaat ttccctgaca ttgcgttgat atgcaccttt gtcactttta
      541 ctcatttctg tataatcacc aatcacaact aattttaaat ttttgtgagc agcggccaat
      601 ttttcgaatg attgcatcaa cagcaatatg cctttgtcag ggacgatacg cccagcataa
      661 aaaataattt tctcttcagg ggagatatta agttcgcttc tagtgatcgg ctgaaaatta
      721 gattgatagg tttctaaatc aattccattt ggtacaatct caatatctgc atttgctaaa
      781 taagattggt aatacttttt caagtataaa ctaggcacta tcattttaac attttgctca
      841 agcaatttt
//
LOCUS       DAJSKJ010000362          843 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000362 DAJSKJ010000000
VERSION     DAJSKJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.368"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(145..669)
                     /locus_tag="ON345_003979"
                     /pseudo
     CDS             complement(145..669)
                     /locus_tag="ON345_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          249 a          188 c          174 g          232 t
ORIGIN      
        1 ctttgccacg gaatggtctg tgttgtcggg aggatacgtg atctgatcct tcaactcagc
       61 aaaagttcga tttattcaac aaagccgttc aaaacaccga aagagataat tgaaaggggt
      121 gttgcattga cagattgaat ctacagctat ctggttgatg ccccatcctt tacctaatag
      181 ccgcagtacc ttgcgctctg aaagagtgag aatgacggag cgatagctaa aatcatgagt
      241 ttcagtcatt gccggagagg tcagcatggc gctgatacgc tctgcgtgcg tattgccgga
      301 acgtacggtc ttgaccagat tctcaatggg ttcaacatca gacaataacg tcgccgtagg
      361 gcacatgagc aattctactg cctgggaata ccaggagcgt gacaccatga aaacccagtg
      421 aatttcccga tattgtgaga ttaaagaata ataatgttca caaacagaac gtggatcttc
      481 actttcaccg gctaaatcag caatgactaa tccggaacga cgtaattgta ataaggtaag
      541 gtcctccgca gaggcgctgg atattatttc atattcaggg aagtgagtcc tcatgacctc
      601 ttttaaccct gcttgcataa caggtatttt actgataaca attccatttt tgcagcatcc
      661 tggcaacata tttcctccac atccactgtc taaaaatcat gatggtttat tcaggatatt
      721 cattccttaa atgcttccag caaattaata gattaagcga ataaattatc cttagcattc
      781 actttcatat cagcatactc ctgctgcaca acaactccac tgcatgtgtc agaaggttag
      841 cat
//
LOCUS       DAJSKJ010000363          842 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000363 DAJSKJ010000000
VERSION     DAJSKJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.369"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            108..>842
                     /gene="fepE"
                     /locus_tag="ON345_003980"
     CDS             108..>842
                     /gene="fepE"
                     /locus_tag="ON345_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR3073941.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLD"
BASE COUNT          246 a          181 c          185 g          230 t
ORIGIN      
        1 tcagtggaag gaatttacaa acttcagctt cggataattc tcaccaactg gtaaagtgag
       61 cgccgtggag attttcccct attggactca ttttcaggtt ataccccatg tcatcattga
      121 atattaaaca gggaagtgac gctcattttt ctgagtatcc tctggcgtcg cccagtaata
      181 atgaaattga tttacttaat ctaatcgaag ttttatggcg ggccaaaaaa acggtcatgg
      241 cggtcgtttt tgcatttgcc tgcgcaggtt tactgatctc tttcatcctg ccgcaaaaat
      301 ggaccagctc agccgttatt actcccgccg aagccattca gtggcaggac ctggagaaaa
      361 cctttaccaa actccgcgtt cttgatctcg atgttaatat cgaccgtggc ggtgcattta
      421 acctgtttat caagaagttt cagtctgtta gcttgctgga agagtacctg cgttcatcac
      481 cttatgtgat ggaccaatta aaagaggcga aaatcgatga actggatttg catcgcgcta
      541 ttgttgcatt gagcgaaaaa atgaaagcgg ttgatgacaa tgccagtaag aaaaaagatg
      601 aaccgtcact gtatacctcc tggacgctaa gttttaccgc gccaaccagt gaagaggcgc
      661 agaccgtttt gagcgggtat atcgattata tctctgcgtt ggtggtgaaa gagtcgctag
      721 aaaacgtccg taataaactg gaaatcaaaa cccagtttga aaaagaaaaa ctggctcagg
      781 atcgcattaa aacgaaaaat caacttgatg caaatattca gcgcctcaat tattcactcg
      841 ac
//
LOCUS       DAJSKJ010000364          825 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000364 DAJSKJ010000000
VERSION     DAJSKJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.370"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>825
                     /gene="rfaL"
                     /locus_tag="ON345_003981"
     CDS             <1..>825
                     /gene="rfaL"
                     /locus_tag="ON345_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR3073942.1"
                     /translation="SLLQSPDAGASMKEIFKAIIENTLLCTIAIPIILRDEKREDVEK
                     IVFFSFISALGLRCFSELITYYKDYQQGIMPFADYRHRSISDSMVFLFPALLNLWLIK
                     SAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIA
                     LSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVY
                     NKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKNGVKNK"
BASE COUNT          248 a          137 c          161 g          279 t
ORIGIN      
        1 tgtcacttct tcaatcccct gatgcaggtg ctagcatgaa ggaaattttc aaagctatta
       61 ttgagaatac tttactatgc acaatagcaa taccgatcat attgagagac gagaaaagag
      121 aagatgtcga aaaaatcgtt ttcttctcat ttattagtgc gttgggctta cgctgttttt
      181 ctgaattgat tacctattat aaggactatc aacaagggat aatgccattc gcagattata
      241 gacaccgtag catttctgac tcgatggtct ttttattccc tgcattgtta aatctctggc
      301 ttatcaaatc agcaaaatac cgcatttctt ttgtggttct aagcgttatt tttatttttc
      361 tgatattagg aactttatcc agaggggcct ggctttccgt gttagtcatt ggattaatat
      421 ggattctgat gtttaaacaa tggaagttac tattagtagg agtaatggtt gccatcattg
      481 cattgtcggt tattttcaca cataaggaga tgaccgcaaa gctaacgtat aaacttcaac
      541 aaactaatag ttcttatcgc tatgcaaatg gtactcaagg cagcgcactc gatctaatat
      601 tagaaaatcc tgttattggt tatggttacg gtaacgttgc atataaagat gtctataata
      661 aacgtgtcat tgattatcca gaatggacct ttagacaatc aatagggcca cataattttg
      721 cgctattcat ctggtttggc actggtttat tagggctggt aagtcttatg atgctatact
      781 gtgcaatatt gaaagagtgt ataaaaaatg gcgtcaagaa taaat
//
LOCUS       DAJSKJ010000365          817 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000365 DAJSKJ010000000
VERSION     DAJSKJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.371"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            3..752
                     /locus_tag="ON345_003982"
     CDS             3..752
                     /locus_tag="ON345_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3073943.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT          215 a          203 c          194 g          205 t
ORIGIN      
        1 cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt caggttagcg
       61 taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag gatctcaaca
      121 ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg cttgatagtg
      181 atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc gccgagtttg
      241 ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggtagc agcaatgccc
      301 tgctcgcccg gactctgggc gaacagaaga aaaatgtcac tatcatcaca gtcagcagct
      361 acattgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt ggcgtgtacc
      421 agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag caggtacatt
      481 tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc accggtcgcg
      541 atatgatgcg taccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa gcgatagtcc
      601 tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc gttgagcgat
      661 tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat cttgagcaca
      721 gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg gatgatttgc
      781 agtttggcaa atcatccgct ctaagatgat tcctagt
//
LOCUS       DAJSKJ010000366          810 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000366 DAJSKJ010000000
VERSION     DAJSKJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.372"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            5..580
                     /locus_tag="ON345_003983"
     CDS             5..580
                     /locus_tag="ON345_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR3073944.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSID
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLQNRVDTSTAQDIEWPALP"
     gene            complement(678..>810)
                     /locus_tag="ON345_003984"
     CDS             complement(678..>810)
                     /locus_tag="ON345_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3073945.1"
                     /translation="EEQKQVVFERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          226 a          161 c          208 g          215 t
ORIGIN      
        1 cataatggca ttcagaatga gtgaacaacc acggaccata aaaatttata atctgctggc
       61 cggaactaat gaatttattg gtgaaggtga cgcatatatt ccgcctcata caggtctgcc
      121 agcaaacagt atcgatattg caccgccaga tattccggca ggcttcgtgg ctgttttcaa
      181 cagtgatgag gcatcgtggc atcttgttga agatcatcgg ggtaaaacgg tttatgacgt
      241 ggcttccggc gacgcgttat ttatttctga actcggtccg ttaccggaaa atgttacctg
      301 gttatcgccg ggaggggaat atcagaagtg gaacggcaca gcctgggtga aggatacgga
      361 agcagaaaaa ctgttccgga tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt
      421 agccagtgag catattgcgc cgcttcagga tgctgcagat ctggaaattg caacggagga
      481 agaaatctcg ttgctggaag catggaaaaa gtatcgggta ttgcagaacc gtgttgatac
      541 gtcaactgca caggatattg aatggccagc actgccgtag ggtaaaacat ataaattcta
      601 taattagatg tatctttcca tttacggcaa ggaaggtggc ttggaagacg taaagcatct
      661 cacaccgaga ttatttttta tatgtcaggt gtctgaagtt ttgctttggc tcttaaaatg
      721 gtttgccgcg aggttttgaa ttcccgggca atggcactta tacttacacc tgacttaatt
      781 cgttcgaata ccacctgttt ctgttcttca
//
LOCUS       DAJSKJ010000367          793 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000367 DAJSKJ010000000
VERSION     DAJSKJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.373"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(81..731)
                     /gene="zinT"
                     /locus_tag="ON345_003985"
     CDS             complement(81..731)
                     /gene="zinT"
                     /locus_tag="ON345_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR3073946.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          226 a          149 c          158 g          260 t
ORIGIN      
        1 gttcagtggc gtttgtggat ggaccagtgg cagttatgat gacttactaa agcggcatcg
       61 aggcgttatc atgagaatac tcaatgagac atcatttcct cgaccacttc ttcgctactc
      121 aactgatatg gataatacgt tggccagttt tccatttcat ttaataaaga ttgctgtgaa
      181 tcattaccca taaaaatgtg aaaatgagat gattttcgtg gtgcaattat atgatcgcta
      241 aattgtatat atttaggggc tttgctttca ggatctttac attcgaataa gtaacgaacg
      301 cctttcttgc ctgatttata ggtgagtatt ttgtatccat cgtaatcata tttacaggat
      361 gttgtttcat tatttctatg gaattcaaca atgccgtcct caatgccaat catctcgata
      421 tctgttgcat aacctttgtg ataataatct ttaatttcag caaatgtttt agttttatct
      481 gcatccgctt tcttctgaaa gacggggtca agtttgccac tttgcagtaa aggataaacg
      541 gattgccaga ctccatccca gtcactgagc gttcggtttt gtacattggc atcatcaaaa
      601 acaccattag cagctttttg ttcgacctct gttaagggtt tgccgtgtga gtgatgacca
      661 tgcgaaaagg caggagcgct aacaataaag acacctaaag caacagccag tttgtgaaga
      721 cgaatcgcca aaacagtttc ctccagagtc attgtatatg atgtgtaatg ttatattgta
      781 acatatggca aaa
//
LOCUS       DAJSKJ010000368          787 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000368 DAJSKJ010000000
VERSION     DAJSKJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.374"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..433
                     /locus_tag="ON345_003986"
     CDS             <1..433
                     /locus_tag="ON345_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR3073947.1"
                     /translation="TPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDD
                     LLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVT
                     QENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            531..>787
                     /locus_tag="ON345_003987"
     CDS             531..>787
                     /locus_tag="ON345_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073948.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSIFQMNRDTTITAGILLKQLDNVTQIVQ"
BASE COUNT          234 a          143 c          189 g          221 t
ORIGIN      
        1 aacgccttat tgcgtcatga ttatggatat cgaccacttc aaaaaagtca acgacacctg
       61 ggggcatccg gttggcgatc aggtgataaa aacagtggtg aatatcattg ggaaaagcat
      121 acgaccagat gaccttttag cgcgcgtcgg cggcgaagag tttggcgtct tgctgacgga
      181 catcgatact gaacgtgcga aagctttagc ggaaaggatt cgggaaaatg ttgagcgttt
      241 aactggcgat aatcctgaat acgctatacc acaaaaagtg acgattagta ttggcgctgt
      301 tgtgactcag gaaaatgcgt taaacccgaa tgagatctat cgactggctg ataacgcact
      361 atacgaggcg aaagagaccg ggcgtaataa ggtggttgtg agggatgtgg tgaatttttg
      421 tgagtcacca taaagcggca ttttagcggg tgattttggt aagcgctaaa tagctattcg
      481 cagacttgcg taacaatttt cagaaatatc atagtactat atgatcttaa atgcttatca
      541 ttgatcaatc tgctatcacc cgcttttact ggcaattgta ctataatttc ttaggttgca
      601 tcaccctgat gcgtggggaa acatgtatgg tattcaacaa aaaaatgttt gtactcatta
      661 taataccagg cattctgggg gtgttactat cgtttgctat gtcaatattt caaatgaata
      721 gagatacgac aattaccgcc ggtattctgt taaaacagct tgataatgtt acgcaaattg
      781 tccagca
//
LOCUS       DAJSKJ010000369          778 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000369 DAJSKJ010000000
VERSION     DAJSKJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.375"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(32..736)
                     /locus_tag="ON345_003988"
     CDS             complement(32..736)
                     /locus_tag="ON345_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCR3073949.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          208 c          200 g          192 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DAJSKJ010000370          771 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000370 DAJSKJ010000000
VERSION     DAJSKJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.376"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            2..>771
                     /locus_tag="ON345_003989"
     CDS             2..>771
                     /locus_tag="ON345_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR3073950.1"
                     /translation="MVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYF
                     IYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSL
                     CILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAE
                     LTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLL
                     FMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVT"
BASE COUNT          201 a          113 c          159 g          298 t
ORIGIN      
        1 tttggttttg gtgaatagtt ggtttaatca gccaggcgtt gaggaagttg ttccacgatc
       61 aacctatctg atggtaatga ttgctttatt ttttatcgat actgttgcat ttatttttat
      121 gcagttgtat ttcatttatg accgtaggca attttcaaac tgtatactta gtttggcttt
      181 tctgagttgt ttgatttact ttgttaaaac cgtcattatc attcagcaaa ttattgaggg
      241 gcgtttgaca agcagtgttg tccaaaatga cattgcaatc tattatttgt ttcgtcagat
      301 gagtttgtgc atattaatat ttctggcatt ggtgaataaa gttagtgaaa acacaaagca
      361 gcgcaattta ttttccaaaa aaatgacttt gtgcataagt ttgttttttg tttttggggg
      421 gccgatagtt gctcacatac tttctagtca ttatgagagc tacaatttac acattgccga
      481 gttaaccaat gaaaatggtc aggtggtttg gaaagcctca tatgttacca taatgatttt
      541 catgtggtta accttgcttt cggttaatct atactttaat ggtttacgct atgatatctg
      601 gaatggagtg acggtgattg ctttttgtgc cgtactgtac aatatctctc tgttatttat
      661 gagcaggtat agcgtgtcca cttggtatat tagtcgcact attgaagtag tcagtaaatt
      721 aaccgttatg gtaatattta tgtgccatat attcagtgcg ctacgagtaa c
//
LOCUS       DAJSKJ010000371          770 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000371 DAJSKJ010000000
VERSION     DAJSKJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.377"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>770
                     /locus_tag="ON345_003990"
     rRNA            <1..>770
                     /locus_tag="ON345_003990"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          216 a          167 c          236 g          151 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcctctttat ggggtgactg
      181 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      241 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      301 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      361 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      421 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      481 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      541 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
      601 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
      661 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
      721 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg
//
LOCUS       DAJSKJ010000372          738 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000372 DAJSKJ010000000
VERSION     DAJSKJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.378"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..397
                     /locus_tag="ON345_003991"
                     /pseudo
     CDS             1..397
                     /locus_tag="ON345_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            complement(434..634)
                     /locus_tag="ON345_003992"
                     /pseudo
     CDS             complement(434..634)
                     /locus_tag="ON345_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          184 a          190 c          156 g          208 t
ORIGIN      
        1 catcgctctc atcaatggcg catcactgac gctgaccagc atcgagcgtt atttacccgg
       61 cctggctcgg gtcaaaaata aaatcaaacg cgttgaccga ctgctcggta atgaatctct
      121 tcaccgcgat atcccacgga ttttcagtaa tattattgcc atgctgacta gccagctatc
      181 tctttgtgtc atcgccgttg actggagtgg atattcatct caggagcatc atgtgctccg
      241 cgccagcctg ctctgcgatg gatgcttgct tcccctgtta agctgggtag tcccgtcaga
      301 aaaacagcag aactcgcaga tacaaaaaaa cttcccagat gcccttgccg aggctgtaaa
      361 ccctgtggcc agagtcataa tagttaccga tgcgggtgtt cttgccccct tatttccaga
      421 caggggctgt atcttaagtt aacattaccc gctgacgtcg atattctcgc ggagagcgat
      481 aactcaacgc actatgcgga tggttttcat tgtaatgctc gaacgcctct gcaaggttct
      541 ttactgccgt taacctgtct ggtttgggca tgatactgat gtaatcgcac tttaccgttt
      601 tcataaagct ctctgctatt ccattactcc gcggctactg ttgaacaggg ttccatatta
      661 tactctattg cgaacaacga aattttatat acctgagaga taaaaaaacg ttctgaacac
      721 tatgcttacg aaatatca
//
LOCUS       DAJSKJ010000373          736 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000373 DAJSKJ010000000
VERSION     DAJSKJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.379"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..498)
                     /locus_tag="ON345_003993"
     CDS             complement(<1..498)
                     /locus_tag="ON345_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR3073951.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYII"
BASE COUNT          201 a          184 c          165 g          186 t
ORIGIN      
        1 aatgatgtaa gagacgccac ccagcgcggc ttttaatttt tccggcgttt ctgtggtaat
       61 acgcgtccca aatccgcctg tcgccatatt aataaaacag gtttgtttgt tgacctgcgc
      121 catatctatc gcaatggcgt taccggcaat tgccagtttc agcgccttat ccagtgcctc
      181 aggaatccct acactggtgg caaaatcatt ggcggttcct aatggcaaaa ttcccagcgc
      241 gggtatgcca tccccctcac actgaatcaa cgccgtagaa acttcattaa tggtgccatc
      301 gccaccaccg gcaatcaccg ttgcgacgcc caacttccgg gcctcctcta catatcgtgc
      361 ggcatcgcct ttctcccagg tgacccgcac atggatcgtc attccttcct cacgcaacag
      421 cataattgct tcgcgcaagg gtagattgtc agtacttttg ccattaagaa tcagtaagct
      481 ggcgggaaat tctgccatga tcgtatgtgc ctttatgatt ggtctgtaga tagtgtagag
      541 cagaaaagaa aaggtggagt cagaacagga tgaaagtcgg aggataaaaa gtcagttcaa
      601 gtatggagga ttcagtggtt accaccagga gcttgcaatg gggacagcga atactaacgg
      661 ctgccaccga taaatttcaa aaaagagcat atacctaata ttcaactaaa cagtggcatc
      721 ttcaatataa tatatt
//
LOCUS       DAJSKJ010000374          736 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000374 DAJSKJ010000000
VERSION     DAJSKJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.380"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..306)
                     /locus_tag="ON345_003994"
     CDS             complement(<1..306)
                     /locus_tag="ON345_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR3073952.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     "
     gene            complement(442..549)
                     /gene="rprA"
                     /locus_tag="ON345_003995"
     ncRNA           complement(442..549)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ON345_003995"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          225 a          136 c          181 g          194 t
ORIGIN      
        1 atagatcttc cagtcaataa aattgccatc agggtagact cgttgaaatg cggttaagtc
       61 ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt attttctcta tgatggcatg
      121 aagatgtttt tttctttgct gacgcatagc caacagttgc tcgactacac gatcaggaat
      181 ggcacacttg ccattttccc actgacgcca gctttcactg tcaccggtct gggcaatcca
      241 ggtcgcgcat tcatcaatag tcatggcaaa aatatggcgg agtgcctgga gttcataagc
      301 gttcatcgtg taatggctgc atgaaataaa caataaaata taaataacat tatcagcctg
      361 ctgacggctt gaagagagtc acagtatctt gtgcaacatt attggaaaga gtgaggggcg
      421 aggtagcgaa gtggaaaaat gttaaaaaaa agcccatcgt gggagatggg caaagactac
      481 acacagcaat tcgttgtttc actcagggga tttccatgct tataaatcaa tatgttgatt
      541 tataaccgtg agctaatagt aggcatacgg actttttgcg tcgatcagat tcgtctcatt
      601 agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg gtaagcagga
      661 aaatatgggg aagagcaagc aaatatcaat tagcgggggt taacaatcag gcggcagagc
      721 cgcctgatga gaaatt
//
LOCUS       DAJSKJ010000375          730 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000375 DAJSKJ010000000
VERSION     DAJSKJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.381"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..719)
                     /locus_tag="ON345_003996"
     CDS             complement(<1..719)
                     /locus_tag="ON345_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR3073953.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGLVVVEKGGEWLIKNNDS"
BASE COUNT          201 a          208 c          130 g          191 t
ORIGIN      
        1 gaatcgttat tttttattag ccactcgcca cccttttcaa cgacaaccag gccattactg
       61 cccgcctgat agcctaaaat ggcgacgatt gaactcgtga cgtaaccctt atccgtaata
      121 tttaatgagc ccgtaccgta actccctatt tcgaataatt cggtcgtcag aacagagtcc
      181 tctccctcaa cattgaccgt cccgacgcct cctgtcgaag aacctaatct taaatagcct
      241 ccatcgacgt gacccttctg tttaatattc agcgtccccg ttccggattg acccacattt
      301 aaaggccttg cattatttcc gctatcatac aatcgccagg tgccacccaa aacattaacg
      361 gtgccctctg agtcctcatt agcgccaatg acgctggtag taatagtggt taaggaggca
      421 ttaccgccag ccaaaatatt aagcactcca gtgccatacc agccaaccag gtaggcatca
      481 taagcaacat cagtatcaat attggtaatc tgatcattat caacttccag acttgcacca
      541 gacgctgaac cgcttagtgc cagcaaaaca cccagcgtca atctactgaa tttcgttgtc
      601 agtccagagg atttacgcaa attaaccgtc gatgttttac ctgccctgcg agttaattcc
      661 gagcaggcct gaaatacctg tagagtgcaa ttccatatca cgcgatagat tctattcata
      721 taaacgctcc
//
LOCUS       DAJSKJ010000376          728 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000376 DAJSKJ010000000
VERSION     DAJSKJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.382"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT          210 a          142 c          157 g          219 t
ORIGIN      
        1 ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc tttcagactg
       61 aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat agattacgag
      121 atgcccagtc tcattgacta tcgtggtgca cctttatacc tgttatacca gatcaaaaat
      181 cacgcaatcc atacaacaaa accagatttg caattcgtgt cacaaaatat gtcgatcttt
      241 ttctaagaag aagatgccat gtgaagccag acgaacactt gcggtggtct tcaaaaaaca
      301 aagattgttg tttagttatc tgttttatat gtgatttatt taggatctaa acaagggaag
      361 ctttgcaagt taactcagtg ggtgtggtga aaataagtgt ttacccgcca tcaggctgag
      421 cataattctc atcatgaaat atgtttcctg gtttgtggct tgtaagtggt cactcctgaa
      481 gtcgatctgg agaggcttgt tgatgttggt gttttcaggg tgatgcctca cttagtttgt
      541 ttgccgtatc gcccggcgaa cagctgtgat tgaggaaggt ttaagtcgta gtgaccaaag
      601 ccatatttac caacggatgc agatgaaaaa atcgtttcct gctttccccc atatctccat
      661 gataaaaagg aatgtaacaa tctcatggcc taaactgacg aaccagccag gaataatcgc
      721 tagggacc
//
LOCUS       DAJSKJ010000377          726 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000377 DAJSKJ010000000
VERSION     DAJSKJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.383"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            124..651
                     /gene="gadE"
                     /locus_tag="ON345_003997"
     CDS             124..651
                     /gene="gadE"
                     /locus_tag="ON345_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR3073954.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT          262 a          125 c          129 g          210 t
ORIGIN      
        1 cgtattagtt cacgaagggt aaagttctta taggcgttta ctatattgaa caacgattcg
       61 gacaaggatg taaataatga aaaggatgac atgttcgaaa cgataacggc taaggagcaa
      121 gttatgattt ttctcatgac gaaagattct tttcttttac agggcttttg gcagttgaaa
      181 gataatcacg aaatgataaa aatcaattcc ctgtcagaga tcaaaaaagt aggcaataaa
      241 cccttcaagg ttatcattga tacctatcac aatcatatcc ttgatgaaga agcgattaaa
      301 tttctggaga aattagatgc cgagagaatt attgttttgg caccttatca catcagtaaa
      361 ctaaaagcta aagcgcctat ttattttgtt agccgcaaag aaagtatcaa aaatcttctt
      421 gagattactt atggtaaaca cttgccccat aagaattcac aattatgttt ttcacataat
      481 cagttcaaaa ttatgcaact gattctgaaa aataaaaatg aaagcaatat cacgtcgacg
      541 ctcaatattt cgcaacaaac attaaagatt cagaaattca acattatgta caaactgaaa
      601 ctaagacgta tgagcgacat cgtcaccctg ggtatcacat cttattttta gtcaggacat
      661 aaacaactga aattgatggc tggcatgacg agggatgcag atgctgattt cattaccccc
      721 ggtgat
//
LOCUS       DAJSKJ010000378          721 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000378 DAJSKJ010000000
VERSION     DAJSKJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.384"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..202
                     /locus_tag="ON345_003998"
     CDS             <1..202
                     /locus_tag="ON345_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3073955.1"
                     /translation="FVPTGENLAIIEHTDVDESLKGQGIGKQLVAKVVEKMRREKRKI
                     IPLCPFAKHEFDKTREYDDIRS"
     gene            429..>721
                     /gene="ghoS"
                     /locus_tag="ON345_003999"
     CDS             429..>721
                     /gene="ghoS"
                     /locus_tag="ON345_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR3073956.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFY"
BASE COUNT          224 a          123 c          163 g          211 t
ORIGIN      
        1 ctttgtgccg accggagaga atttagcgat tatcgaacat accgatgtcg atgaaagcct
       61 gaaagggcaa gggattggta aacagctggt tgcgaaagtc gtggaaaaaa tgcgtcggga
      121 aaaacgaaaa attatcccat tatgtccatt tgcgaaacac gaatttgata aaacgcggga
      181 gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt cttgctctcc
      241 gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat gggcagagag
      301 cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata tctcctatat
      361 gagaatcatc aatcggggtt aataagtttt gcgtccccag agcattcaat attgatagga
      421 gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt gattatccat
      481 catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg aatttctctt
      541 ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaattcc ttcgcaataa
      601 cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc ttagacttac
      661 cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt cagttttaca
      721 a
//
LOCUS       DAJSKJ010000379          721 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000379 DAJSKJ010000000
VERSION     DAJSKJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.385"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            41..>721
                     /locus_tag="ON345_004000"
     CDS             41..>721
                     /locus_tag="ON345_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3073957.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKTKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDD"
BASE COUNT          239 a          126 c          159 g          197 t
ORIGIN      
        1 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
       61 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
      121 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
      181 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
      241 aattgataaa accaaaggcg gcggagatga aacgttcgta cagggacgtt atgaaggctt
      301 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtaa accgtacgat
      361 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
      421 cagctatatg tttgacaata ctggtgtgat tgtatttaaa ggatcagacc ctgaccatat
      481 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
      541 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
      601 tggaatcacg gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
      661 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
      721 t
//
LOCUS       DAJSKJ010000380          719 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000380 DAJSKJ010000000
VERSION     DAJSKJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.386"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..564)
                     /gene="yeeO"
                     /locus_tag="ON345_004001"
     CDS             complement(<1..564)
                     /gene="yeeO"
                     /locus_tag="ON345_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR3073958.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAG"
BASE COUNT          225 a          188 c          157 g          149 t
ORIGIN      
        1 ccctgcacca cgaagtgccc cgctaccaat aagagtgatg gcagctgctg gataactgag
       61 tacggtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg catcacccgc
      121 gacgaaatca ataatttgtt cgccaaaatg atgaattagc gttgccaaca gtacggcaaa
      181 caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt
      241 accgagacta aatgctacaa cgacagtagt accaagatcg atagctgcaa aaaaagccat
      301 aatgaccata ttgaagctgt ccgccaaccc cacgccggcc atcgcatctt ttcccagcca
      361 gctgaccaga aaagtgctca ggacccccat caacaggaca caggcgttct ccatgaagat
      421 aggaacagca agcggggtta tctcgcgcca gaacaacact ttgtagctct tgcgtttaga
      481 gtgccagcga gtaccgtgaa caacctggcg taaagcagag gagatattca aagccgacct
      541 taattgcaga aagtgaaacc acatttcaaa tgatgaggga gaatcagcaa agctgcaaag
      601 attttcgcca acaaattgtc tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt
      661 gggaaattta aaaatcgggt ttgacaaaat atttttcgcc gttaagatgt gcctcaaca
//
LOCUS       DAJSKJ010000381          710 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000381 DAJSKJ010000000
VERSION     DAJSKJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.387"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(1..302)
                     /locus_tag="ON345_004002"
                     /pseudo
     CDS             complement(1..302)
                     /locus_tag="ON345_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(558..>710)
                     /locus_tag="ON345_004003"
     CDS             complement(558..>710)
                     /locus_tag="ON345_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR3073959.1"
                     /translation="IFSRYLLENKHLFYQYFKISNTGIYHLEQLINVNFFSSDRTSFC
                     ECNRFP"
BASE COUNT          225 a          135 c          131 g          219 t
ORIGIN      
        1 atataatcgt catgttctcc tgtagaaata tctatgatga atggactcat attttccggt
       61 tggattcgga tcatgacaga aggaacatcg ccttgataga aattcaatgc agtaattgca
      121 gataatactc accgcagttc attacttata ctgtaaattt tattaacgtc attggtggag
      181 acaatttctg caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta
      241 accactgcgt gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc
      301 attgatgcct gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcacgac
      361 acttacgcac tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc
      421 tgtgtcctac gctgatgtaa agcacagtga aaatccccct tgttgacttc gtctgacctt
      481 atcgcggcga ccagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc
      541 tatgagggga gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa
      601 gagaaaaagt tgacgttaat caattgttcg agatgataaa tacctgtatt cgaaatttta
      661 aaatattgat aaaataaatg tttgttctcc aacaaatagc gtgaaaaaat
//
LOCUS       DAJSKJ010000382          708 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000382 DAJSKJ010000000
VERSION     DAJSKJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.388"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>708)
                     /locus_tag="ON345_004004"
     CDS             complement(<1..>708)
                     /locus_tag="ON345_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073960.1"
                     /translation="NYLPESPIVNNLEALAPGIEKLKQTSIEMVTLLNALQPGGKCVI
                     TGDFQKELAYLQNVILYNDSSLRMDFFGYNALIIQRSDNTCELTINEPLKNQEISTGN
                     INVNFPLKDIYNEISRLNVVFSCGTGGIVDLSSLDLRNIDLELYDFTDKHMANAILNP
                     FKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPVLTSISDMYSEEKIKLVESCL
                     NELGDITEEQLKIMRFAI"
BASE COUNT          225 a          114 c          118 g          251 t
ORIGIN      
        1 aattgcaaat ctcatgattt ttaattgttc ttcggtaata tctccaagct catttaaaca
       61 actttcgaca agcttaattt tttcttcaga atacatatcg ctaatgcttg ttagaacagg
      121 agttattttt agtaaataat cccaggagat tgtggtgttt tgttttgaac taacgaaatt
      181 tacctgaaac atgttggcat tagtaaaatc tgtgtcatcc aatttaaaag gatttaaaat
      241 tgcattggcc atatgtttat ctgtaaaatc ataaagttct aaatcgatat tccgtaagtc
      301 cagagaggat agatcaacta tacctccagt cccacaacta aaaactacgt tcaacctact
      361 gatttcattg taaatgtctt tcaatgggaa attaacattg atattgcctg tggatatttc
      421 ctggtttttt aacggttcat taattgtaag ttcacaggta ttgtctgatc gttgaataat
      481 tagggcgtta taaccaaaaa aatccatacg aagagacgaa tcattataaa gaattacatt
      541 ttgtaagtac gctaattctt tttgaaaatc accagtgata acacatttcc cacctggttg
      601 taacgcgtta agtaaagtga ccatttcaat agaggtctgt tttagttttt ctatacctgg
      661 tgctagtgct tcgaggttat taactatggg gctttcagga agataatt
//
LOCUS       DAJSKJ010000383          697 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000383 DAJSKJ010000000
VERSION     DAJSKJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.389"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            124..459
                     /gene="eutS"
                     /locus_tag="ON345_004005"
     CDS             124..459
                     /gene="eutS"
                     /locus_tag="ON345_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR3073961.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIASDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            472..>697
                     /locus_tag="ON345_004006"
     CDS             472..>697
                     /locus_tag="ON345_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR3073962.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGAN"
BASE COUNT          184 a          157 c          177 g          179 t
ORIGIN      
        1 acagtaaata tctcttcatt cttagtgatc tacctcacct tttaaacgcg cttgccgaat
       61 tttgttattt actctgacga aaaattgtca cgatacacgc aagtttttca caggcggcga
      121 ctcatggata aagaacgcat cattcaggaa tttgtgcccg gcaaacaggt cacactggcg
      181 catctcatcg cgcatcccgg cgaggaactg gcgaagaaaa tcggcgttcc ggatgcgggc
      241 gcaatcggca ttatgacgct aactcccggc gaaacggcga tgatcgccag tgatttagcg
      301 ctgaaagccg ccgatgtgca tatcggtttt ctcgaccgct tcagtggtgc attggtgatt
      361 tatggctcgg tgggtgcagt agaagaagcg ttatcacaaa ccgtcagtgg tttaggtcgc
      421 ttattaaatt atacgctttg tgaaatgacc aaaagttaat acgaggcatt catgaaacga
      481 attgcgtttg tgggtaccgt cggggcaggg aaaacaacgt tatttaatgc gttgcagggg
      541 aattataccc tcgccagaaa aacacaggcc gtggaattta atgataaggg cgatattgat
      601 acgccgggtg aatattttag ccatccccgc tggtatcacg ccttaattac cacgctacag
      661 gatgtggaca tgctgatcta tgtccacggc gcgaatg
//
LOCUS       DAJSKJ010000384          666 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000384 DAJSKJ010000000
VERSION     DAJSKJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.390"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     repeat_region   133..649
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=255..283
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT          143 a          174 c          183 g          166 t
ORIGIN      
        1 ttttttgtat cgataattgt gaacctctct ggcatggaga actattttga acatgaggtg
       61 ttacgtggat atgttgctta ttacaagtac tgctaatata aaaacttgag aaagagataa
      121 cgggttatat ggtggtttat ccccgctggc gcgggaaact caacatcgga aacggcttcg
      181 cggcggcggc gtccggttta tccccgctgg cgcggggagc tctacgtgaa gaatatttgc
      241 aacacccgca agaacggttt atccccgctg gcgcggggaa cactcagaaa gctaattaaa
      301 gtattcatat aaatccggtt tatccccgct ggcgcgggga acacattgat acgtggatcc
      361 gcgctggctg cccgtacggt ttatccccgc tggcgcgggg aacactgaag tcaatgcctg
      421 caattctcga catgctgcgg tttatccccg ctggcgcggg gaacacctcg ctgccgtcgt
      481 ttaccgtgat ttcgtagccg gtttatcccc gctggcgcgg ggaacacgtt tcacgacgtc
      541 ccacggtggc aaattccggc ggtttatccc cgctggcgcg gggaacacgg aatccgtttc
      601 gcgcaattcg tattcgttgc cggtttatcc ccgctggcgc ggggaacact ctaaacataa
      661 cctatt
//
LOCUS       DAJSKJ010000385          660 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000385 DAJSKJ010000000
VERSION     DAJSKJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.391"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>660
                     /locus_tag="ON345_004007"
     CDS             <1..>660
                     /locus_tag="ON345_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073963.1"
                     /translation="IEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTGSVTV
                     SAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGDYTDSAELRLVLHDISGNPIK
                     VSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTGGGEGIATLIPVLNGVHQAGL
                     STTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEF
                     S"
BASE COUNT          197 a          149 c          163 g          151 t
ORIGIN      
        1 gatcgaaggt ctcactgtgt acttcgcctt aaaaagcggc tctgccacat taacgtcatt
       61 aacagcggtg accgatcaaa acggaatcgc gacaacaagc gtgaaaggag cgatgacagg
      121 tagcgtcacg gtaagcgcag tcacgaccgc tggtggaatg caaacagtag atataacgct
      181 ggtggctggc ccggcagaca cctcgcagtc cgtccttaag agcaatcggt catcactgaa
      241 aggggactat accgatagtg ctgaattacg tcttgttctg cacgatatat caggcaatcc
      301 gatcaaagtt tctgaaggga tggaatttgt gcaatcaggt actaacgtgc cctatataaa
      361 aattagcgca attgattaca gtctaaatat caacggtgat tacaaagcca ctgttacagg
      421 aggcggagag ggtatcgcaa cgctgatccc tgtattgaat ggtgttcatc aagctggtct
      481 gagtaccaca atacaattca ctcgcgcaga agacaaaata atgagcggta cagtatcagt
      541 caatggtact gacctaccga caactacatt cccttcgcag gggttcaccg gggcgtatta
      601 tcagttgaat aatgacaact ttgccccagg aaaaacggcg gctgattatg agttttcaag
//
LOCUS       DAJSKJ010000386          655 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000386 DAJSKJ010000000
VERSION     DAJSKJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.392"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..625
                     /locus_tag="ON345_004008"
     CDS             <1..625
                     /locus_tag="ON345_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR3073964.1"
                     /translation="VIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVK
                     IMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDV
                     VLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNY
                     NYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT          210 a           92 c          138 g          215 t
ORIGIN      
        1 tgttattgct gaagaatatt caacaatgcc tgtgaaaaat ttctccagtg tcggcgcttt
       61 ttcatttccg acctgccatt tttcagggaa tatacgtata ggtcgatttt gtagtattgc
      121 atcaaatgta aaaataatgg gtggaaatca tcctttgaat cgttttacta ctcacatgat
      181 gacttataac ggtgaatttg ataagtttgc tatgagtgaa tttgaacgta gctggacatt
      241 gaaaccattc attaccaaac ctgaaaatcc gattatagga aatgatgtct ggataggaaa
      301 tgatgttgta ttgaaaggtg gcatcgcaat aggtgatggt gctgttattg ctgccaattc
      361 cgttgttacc aaagatgtgc ccccatatgc catagttgca ggagttcctg caaaaataat
      421 cagatttagg tttgattcaa atgttattga tgaactgttg agaataaaat ggtggaatta
      481 taactactca gatcttccgg ataataacaa gtgtgatgat ataaattatt ttgttgaaga
      541 gatgaatcga ttgatatcag atggaaatat tcaagagagg gactataaaa aattcaatct
      601 ttctgaggta ttcagaggcc tataattatc tgttttgcat gataaatggg caaga
//
LOCUS       DAJSKJ010000387          649 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000387 DAJSKJ010000000
VERSION     DAJSKJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.393"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            50..616
                     /gene="slp"
                     /locus_tag="ON345_004009"
     CDS             50..616
                     /gene="slp"
                     /locus_tag="ON345_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR3073965.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          187 a          138 c          147 g          177 t
ORIGIN      
        1 taaagaaata tctattatgg ttttaatatt tgttgataag gatagtaaca tgaacatgac
       61 aaaaggtgca ctcatcctca gcctttcatt tttacttgcc gcatgtagtt caattccgca
      121 aaatatcaaa ggcaataacc aacctgatat tcaaaaaagt tttgttgctg ttcataacca
      181 gccggggtta tatgttggtc aacaagcgcg ctttggtggg aaggttatca acgttatcaa
      241 tggcaaaacg gatacgttgt tagaaatcgc tgtattaccg ttggatagct atgcgaagcc
      301 tgatattgaa gccaactatc agggccgact gctcgccaga caaagcggct tccttgatcc
      361 agtgaactat cgtaatcact ttgttaccat cctcggtacc attcagggtg aacaaccagg
      421 ctttatcaat aaagtcccgt ataacttcct ggaagtgaat atgcagggca tccaggtgtg
      481 gcatttgaga gaagtggtta ataccaccta taacctgtgg gattacggct atggtgcatt
      541 ctggccggaa ccgggctggg gtgcgcctta ctacaccaat gcggtgagtc aggtaacacc
      601 tgagctggtc aaataacacc accgaaagat gcagatgctt tctcagcat
//
LOCUS       DAJSKJ010000388          640 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000388 DAJSKJ010000000
VERSION     DAJSKJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.394"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>640)
                     /locus_tag="ON345_004010"
     CDS             complement(<1..>640)
                     /locus_tag="ON345_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR3073966.1"
                     /translation="LESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRN
                     DGILIIVAWSDRTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINA
                     LNNQLTSQHQYPTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSG
                     VYISAIYLLDLFLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHS"
BASE COUNT          220 a          114 c          123 g          183 t
ORIGIN      
        1 ctgaatgata acttttacct gatgctcttt tctgaagtac acataaacag gtaaagacca
       61 taaatatggt gaaagtaaaa aagaaggaaa atacagcaat ggaaaaaatt ttatcggaga
      121 acaggaataa atctaaaaga tagattgctg aaatatagac tccactttta catgcgcgac
      181 aaaaattact gccttgatga aaaaaccaga aagggaagaa catcactgca aatacccaaa
      241 aaatagcaaa gtaataatga tcactggaag acgctgattc actttctgaa ggattagtcg
      301 gatattggtg ttgtgaagtg agttggttat tcaaagcgtt tatagctagc tctaatccct
      361 gagcaaattt ttgttgttta aatgcgggta tcatgttgct acggatgata tctccagcca
      421 gagcatcggt taccttttct tccagcccat aacctacctg gatgcggaca gtacgatctg
      481 accaggcaac aatgatcagt atcccatcat tacgtttggc atctcctaaa cgccaattgt
      541 caaaaactct tgtagcatat tgttcaattg tctcgtcttt ggttgtggga acaattaata
      601 cagcaatttg cacttgggta ttattgaaaa tagattctaa
//
LOCUS       DAJSKJ010000389          625 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000389 DAJSKJ010000000
VERSION     DAJSKJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.395"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            8..571
                     /locus_tag="ON345_004011"
     CDS             8..571
                     /locus_tag="ON345_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR3073967.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT          184 a          141 c          142 g          158 t
ORIGIN      
        1 acaaataatg agtaagtttg ttaaaacagc tattgctgcg gcaatggtga tgggcgtttt
       61 cacctctacg gcaacaatcg ctgcgggtaa caacggtact gcacgttttt acggcaccat
      121 tgaagactca gtgtgttcta tcgttccgga cgatcacaaa ctggaagttg atatgggtga
      181 tatcggtgcc gaaaaactga aaaataacgg caccaccacg ccgaaaagtt tccagattcg
      241 tctgcaagat tgcgtatttg atactcagga aacaatgacc actaccttta ctggtaccgt
      301 ttcttctgca aatagcggca attattacac cattttcaat accgataccg gtgcggcatt
      361 taacaatgtt agcctggcga tcggtgactc tctgggtacc tcttacaaaa gcggcatggg
      421 tattgaccag aaaatagtga aagacacttc tactaataaa ggcaaagcga agcagacact
      481 gaactttaaa gcctggctgg tcggcgcagc tgatgcgcca gatctgggta atttcgaagc
      541 aaataccacc ttccagatta cttacctgta aggcataccc ccatagcgac actgctatgg
      601 gggatttaaa tggattttga ccgaa
//
LOCUS       DAJSKJ010000390          621 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000390 DAJSKJ010000000
VERSION     DAJSKJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.396"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            9..559
                     /locus_tag="ON345_004012"
                     /pseudo
     CDS             9..559
                     /locus_tag="ON345_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
BASE COUNT          185 a          132 c          144 g          160 t
ORIGIN      
        1 ggaatattat gtctgctttt aaaaagtcac ttcttgttgc tggcgtagca atgattcttt
       61 ccaataacgt atttgctgac gaaggtcacg gcattgttaa atttaaaggt gaagttatct
      121 ctgctccatg ttcaattaaa ccaggtgacg aagacttaac tgtaaatctg ggtgaagtag
      181 cagataccgt tctgaaaagc gaccagaaat ctttggctga gcctttcact attcacctgc
      241 aagactgcat gttgagccag ggtggaacca cttattcaaa agcgaaggtt actttcacca
      301 cagcgaatac tatgacgggt cagactgacc tgctgaaaaa caccaaagaa actgaaatcg
      361 gtggtgccac aggcgttggc gtacgtattc tggatagcca gagcggcgaa gtgactttag
      421 gtactccggt agtaattaca ttcaacaata ctaacagcta tcaggaactg aacttcaaag
      481 ctcgtatgga atcccgggca aagatgctac cccgggcaac gtttacgctc aggctgacta
      541 taagattgct tacgagtaat cacattaggc cgggttcgcc cggccacatc attaacggat
      601 taatgataag tggaacagat g
//
LOCUS       DAJSKJ010000391          614 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000391 DAJSKJ010000000
VERSION     DAJSKJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.397"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            175..>614
                     /locus_tag="ON345_004013"
     CDS             175..>614
                     /locus_tag="ON345_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR3073968.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQS"
BASE COUNT          211 a          136 c          154 g          113 t
ORIGIN      
        1 gaaggtgttt ctttaacata taaggaaggc accaaagtgt atacctctac ccaggtgggt
       61 aaagagtgcc agtttacgac aggtttagcc gttgttatta ccacaacgta taacgaaacg
      121 cgtattcagc caaatactaa atgtcctgaa aagagctaat aatcaggagg agtcatgaag
      181 aaagtttttc tttgcgccat cttggcctcc ttaagctatc cggctatcgc ctcatcattg
      241 caggatcaac tctctgctgt agcagaagcg gaacagcaag gtaaaaatga agagcaaagg
      301 cagcatgacg aatgggtcgc ggagcgcaac agggaaatcc agcaagagaa gcaacgtcgc
      361 gcaaatgccc aggccgccgc taacaaaaga gcggcaacgg cagcagcaaa taagaaagct
      421 cgtcaggata aactggacgc cgaagcctct gcggacaaaa aacgcgatca aagttatgaa
      481 gatgagctac gcagcttaga gattcagaaa caaaaactgg cgctggcaaa agaagaagcc
      541 cgcgttaagc gagaaaacga atttatcgat caggaactga agcacaaagc tgcgcaaacc
      601 gatgtggtgc aatc
//
LOCUS       DAJSKJ010000392          614 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000392 DAJSKJ010000000
VERSION     DAJSKJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.398"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..247
                     /locus_tag="ON345_004014"
     CDS             <1..247
                     /locus_tag="ON345_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR3073969.1"
                     /translation="ANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYARL
                     GFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            271..>614
                     /locus_tag="ON345_004015"
     CDS             271..>614
                     /locus_tag="ON345_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR3073970.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINL"
BASE COUNT          218 a          131 c          104 g          161 t
ORIGIN      
        1 tgcgaacgta gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta
       61 tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac
      121 ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg
      181 tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaagaa
      241 tctctgattg agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat
      301 cccaggatga aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac
      361 actacaaaac tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt
      421 gatcaactaa tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat
      481 ccacttgcac aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa
      541 gtgaccattg atttatcagg acttaattta accactcaac caccagggct gcaaaacttc
      601 acctctatca atct
//
LOCUS       DAJSKJ010000393          600 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000393 DAJSKJ010000000
VERSION     DAJSKJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.399"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            8..>600
                     /locus_tag="ON345_004016"
     CDS             8..>600
                     /locus_tag="ON345_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073971.1"
                     /translation="MLFFHESWDIMVIKNDGSHSFPDNTLDILRRSTAQRLKKKFTFQ
                     ADPFLLEKEDKLYIFYEAFNFRNSKGILRCRILDKNLKELDDVKLEGFDNLKCHLSFP
                     FLFTTNGTLYMIPESSERKEVILFQAIDFPVRWKQVKVLLSDAPLTDNVLLTVNERFY
                     LMSTSMENELVIHTAPEVTGKWEKITPLLEVCNEHNR"
BASE COUNT          189 a          112 c          117 g          182 t
ORIGIN      
        1 attaaatatg ttattctttc atgagtcttg ggatatcatg gttattaaaa acgatggttc
       61 tcattccttt cccgataaca ctctggacat actgagacgt tcaacagcac aaagattaaa
      121 aaagaaattc acttttcagg ccgatccgtt tcttcttgag aaagaggata agctgtatat
      181 tttttatgag gcatttaatt ttcgcaactc aaagggtatt ctgagatgcc gtatcctgga
      241 taaaaacctc aaagaactgg atgatgtgaa actggagggg tttgataatt taaaatgcca
      301 tctgtcattt ccattcctgt tcaccacaaa tggaacttta tatatgatcc cggaatcctc
      361 agaaagaaaa gaggtgatat tattccaggc gatcgatttc ccggtgcgct ggaaacaagt
      421 caaggtttta ctctcagacg ctccgcttac cgacaatgtg ttacttactg ttaatgaacg
      481 cttttattta atgtcgacct cgatggaaaa tgaattggtc attcataccg cgcctgaagt
      541 aactggaaaa tgggaaaaaa taacgccatt acttgaagtc tgtaatgaac acaatcgtag
//
LOCUS       DAJSKJ010000394          590 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000394 DAJSKJ010000000
VERSION     DAJSKJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.400"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..126
                     /locus_tag="ON345_004017"
     CDS             <1..126
                     /locus_tag="ON345_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3073972.1"
                     /translation="RFAWPSARDGKVFLTPAQLAMLLEGIDWRQPKRLLTSLTML"
     gene            175..>590
                     /locus_tag="ON345_004018"
     CDS             175..>590
                     /locus_tag="ON345_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3073973.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQL"
BASE COUNT          136 a          179 c          164 g          111 t
ORIGIN      
        1 cgcttcgcct ggccgtcagc ccgggatggc aaagtgttcc tcacaccggc acagctggcg
       61 atgctccttg aaggtatcga ctggcggcag cctaaaagac tgcttacgtc cctgactatg
      121 ttgtaagcct ctttatcctg gtcgacgctg aatgagcctg gtaatatacc cggtatgagc
      181 agctcacttc ctgacgatat caatgcactg aaacgtctcc ttgccgaaca ggaggcgctg
      241 aaccgtgccc tgctggaaaa gctgaacgag cgtgaacgcg aaatagacca tctgcaggca
      301 cagctggata agctgcgccg gatgaacttc ggcagccgct ccgaaaaagt ctcccgtcgt
      361 atcgcacaga tggaagctga cctgaaggca cttcagaaag aaagtgatac ccttaccggt
      421 cgggttgacg acccggccgt gcagcgcccg ctgcgtcaaa cccgcacccg caaaccgttc
      481 cccgaatcac tcccccgcga tgaaaaacgg ctgctgccgg cagcgtcatg ctgcccggaa
      541 tgtggaggct cactgagcta tctgggtgag gatgccgccg aacagctgga
//
LOCUS       DAJSKJ010000395          587 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000395 DAJSKJ010000000
VERSION     DAJSKJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.401"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>587)
                     /locus_tag="ON345_004019"
     CDS             complement(<1..>587)
                     /locus_tag="ON345_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR3073974.1"
                     /translation="LNDRDFIASVVDRAIFHFAINSICNPGDNKDAMPIEQCTFDVET
                     KNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLLISL
                     SYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYLD
                     KMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPT"
BASE COUNT          184 a          122 c           98 g          183 t
ORIGIN      
        1 gttgggtcaa acgacttcgc gaagctaatt aattgtggtg cagcatctcg cattttgctt
       61 aaaatatcag gatcgagcgt tccttcattc accagatgtg acatcttatc cagatagcca
      121 tcaaaattca tttctccccc atcagccaga cgcttcatct ctcccaggta cttcttcata
      181 tcacgttgag ataatttttc aaactgagct ttcaggtagc tctcattatt ttcattaata
      241 tacactgttt ctgataaatc tgcgtaggca ctagaatatg aaagtgaaat taacagcgaa
      301 gcaaataatt ttaagctgtt gtcatcatgt tcctggcagg cattaacaaa cgttaaaaaa
      361 ccagaaccta ttgcttgaaa atgtatattc gctaaaggtt cattatcctt agattcctca
      421 taaaatagct gaaccgtgga gggaaggtca ttcttagttt ctacatcaaa agtacattgt
      481 tcaatgggca ttgcatcttt attatcccca ggattacata tactattaat cgcgaaatga
      541 aaaatagccc ggtcaacaac tgatgcaata aagtcacgat cgtttaa
//
LOCUS       DAJSKJ010000396          585 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000396 DAJSKJ010000000
VERSION     DAJSKJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.402"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>585
                     /locus_tag="ON345_004020"
     CDS             <1..>585
                     /locus_tag="ON345_004020"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3073975.1"
                     /translation="KKLIYSFVGLGRVFENSSIVYRALRFVVIRLYRTLFTAIDCKII
                     FEHEADQKKIINLIGLDQSKTIVIDGAGIDINHFTFSEEQQSEPPKILFASRMLWSKG
                     LGDLVQVKNILKHDGLDIEILVAGIIIENDDDAISLNQIQKWNDSGDIVWLGTRNDVK
                     ELIQLSNIVALPSIYGEGVPRILLEAGAVGRAAIT"
BASE COUNT          181 a           94 c          128 g          182 t
ORIGIN      
        1 aaaaaactta tctatagctt tgtcggatta ggacgcgtat ttgagaatag ctctatagtt
       61 tatcgagcgc tgcgttttgt tgtaattcga ttatatcgta ccttattcac ggcgatagat
      121 tgtaaaataa tattcgagca cgaggctgac caaaagaaaa tcattaattt aattggatta
      181 gaccagtcta aaacaattgt gattgatggc gctggtattg atatcaatca ttttacgttt
      241 agcgaagagc aacaaagtga accgcctaaa atcttatttg ccagccgaat gctatggagt
      301 aaaggtcttg gagaccttgt acaggttaaa aatatcctaa agcatgacgg tttagatatc
      361 gaaattttag ttgctggaat cattattgag aatgatgatg atgctataag tctaaatcaa
      421 attcagaaat ggaatgattc tggtgacatt gtatggctgg gaacgcgtaa tgacgtcaaa
      481 gaacttatcc agctatcaaa tattgtcgcc cttccttcaa tatatggtga aggtgtccca
      541 cgtatcttac ttgaggcggg ggctgttgga cgagctgcta ttact
//
LOCUS       DAJSKJ010000397          577 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000397 DAJSKJ010000000
VERSION     DAJSKJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.403"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            73..>577
                     /locus_tag="ON345_004021"
     CDS             73..>577
                     /locus_tag="ON345_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3073976.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGN"
BASE COUNT          166 a          114 c          134 g          163 t
ORIGIN      
        1 attattgtta ttattaagtc taatgtcatt taaaggcatt atcatattta cgaagagatc
       61 aggatgatga cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg
      121 tccagcgtta tggctgctga tgataatgct atcacggatg gctcagtaac atttaatggt
      181 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
      241 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
      301 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
      361 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
      421 gcgacgaatg ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt
      481 accgaaacgt cgaatattca gttagcggat tctgctgagc aaacagtgac ctttaaagtt
      541 gattatatcg ctacggggaa agcgacttct ggtaatg
//
LOCUS       DAJSKJ010000398          571 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000398 DAJSKJ010000000
VERSION     DAJSKJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.404"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..559)
                     /locus_tag="ON345_004022"
     CDS             complement(<1..559)
                     /locus_tag="ON345_004022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073977.1"
                     /translation="MLTSPNNEEIHYYYLWFLSAANISLLTNISGWAADIVLTFITAL
                     TSLFVVCGLIKENSSGKLSTYVIGISSLFGSQIVTYVDRITQGQIFEHISREHSVESW
                     LIQASWVPQHLMAASVVITSCYLIFSINKNSRWISSLVISILISTGVGSSTWVGGVTF
                     AVISFCLFIFDLITNKSEWKFTLSKW"
BASE COUNT          213 a           95 c          101 g          162 t
ORIGIN      
        1 cccattttga taaagtaaac ttccattctg atttgtttgt aatcaaatcg aaaatgaata
       61 aacaaaagga aattactgca aaagttacac cgccaaccca agttgatgag ccaacgccag
      121 tagaaattag aattgatata acaagtgaac taatccatct actatttttg ttaatcgaaa
      181 aaattaaata acaactcgta attactacag aggctgccat taaatgttgt ggaacccaac
      241 tagcttgaat taaccacgac tcaacagagt gttcgcgtga aatatgttca aaaatttggc
      301 cttgtgtaat cctgtctaca tatgtgacaa tttgagaacc aaaaagtgaa gagataccaa
      361 tgacgtaagt gcttaatttt cctgagctat tttctttgat aagaccacat actacgaata
      421 aggaagttaa agcagttata aaagtcaaaa caatatcagc tgcccaacca gaaatgtttg
      481 tgagcagaga tatgttcgca gcagaaagga accaaagata atagtaatga atttcttcgt
      541 tgtttggaga ggttaacatg aatgggtttt t
//
LOCUS       DAJSKJ010000399          562 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000399 DAJSKJ010000000
VERSION     DAJSKJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.405"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT          186 a          120 c           80 g          176 t
ORIGIN      
        1 gatctttgtt tgttataaca acaagtaaat cgtacagata tcctaaatta tacttcatca
       61 tcaatgctca ttagacatgc ctgctataat gcatgaatcc agttcaatta gtaaaaaaag
      121 ggtacgctac cgcccttggc tcagagaaac caaatacctc accactagtc ccgttgctgg
      181 catagcatag ctctatgcac tcgcattgtt gtcaatatga cgtaaatagc tacttttaca
      241 catgacttag cataaaatcc ccaccaaagt catgttgtca agaaattttc taataattgt
      301 aaagtctcga ttatcacaaa ttatcaattg gttacaacac tcattcgttt gatgataaaa
      361 tgctcaagtt atcaatgaat ttgacagttt attatagact tttcttactc acaatccagc
      421 ttaacgtagc tttttgaaca ataaacatac agacaatgaa tatctctttg ctcgtaactg
      481 caatggctgg aacaaagttg tttcgcatgt gactcacaaa gactacctac aaacatcttt
      541 tgctcaatgt tcgttaccca ag
//
LOCUS       DAJSKJ010000400          552 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000400 DAJSKJ010000000
VERSION     DAJSKJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.406"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            29..552
                     /locus_tag="ON345_004023"
                     /pseudo
     CDS             29..552
                     /locus_tag="ON345_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          162 a          132 c          125 g          133 t
ORIGIN      
        1 cataatctga catctgaagg tgcagtttat gaaacattca tttgaagtaa aacttgccgc
       61 tgtaaatcat tacctggctg gtcatgcagg catcatttct acggcaaaac tctttcaact
      121 ttctcacacc agcctctcac actggattaa tctttttctt cttcacggtc ctcgggcact
      181 ggattgcaga cacaagcgta gctattctcc tgaagataaa ctttgcgtgg ttctttatgc
      241 tctcgggcat tctgagtctc taccccgggt agctgcccgg tttaacattc ccagccacaa
      301 cacggttaaa aactggataa aaggctaccg caaatctggt aatgaggcct ttatcaggcg
      361 caggaaggag aaaagcatga cccgttctga tgatacccat gaaaacgagg caaacatgac
      421 gccggaagag atgaagaatg agctgcgtta tcttcgcgca gaaaatgcat atctgaaagc
      481 catgcaggag catctcctgg aaaaaaagcg ccaggagctg gagaaaaaac gaaagtcatc
      541 cggagcctga gg
//
LOCUS       DAJSKJ010000401          547 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000401 DAJSKJ010000000
VERSION     DAJSKJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.407"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..214)
                     /locus_tag="ON345_004024"
     CDS             complement(<1..214)
                     /locus_tag="ON345_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073978.1"
                     /translation="MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIV
                     GAFIFGKMGDRIGRKKVLFITITMMGI"
BASE COUNT          157 a          136 c           90 g          164 t
ORIGIN      
        1 agatccccat catggtgatg gtaataaaga gcactttttt acgccctatt ctgtcgccca
       61 ttttgccgaa aataaatgct ccgacaatac gcgccacata acctgcaccg taggttccca
      121 ttgccaaaat taacgccatt gccgttgatg attcaggaaa aaatatttca tgaaacacta
      181 acgctgcgcc gagcgaatat aactggaaat ccataattca caggtgtttt ttcccatcct
      241 gtggtttcct tggcgttttc taggtttttt cagatagttg cattttttaa aaagcatcct
      301 aagttcgatc tcagtgtcta tctggggcct atttctgtcc catatatgcc ccaaaaaaaa
      361 ctccccaaca gataagtagt tttttcatgg atttatgcgt aaaatcaaga acggctggaa
      421 atcattcaat actcacacta tcgaaaaatt taccagccaa ccgcagcacg ttcttgcata
      481 aggcgtgtct gcggtttttc aactattcag atacatcact cccatcacat tcattcctcc
      541 gcatcaa
//
LOCUS       DAJSKJ010000402          545 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000402 DAJSKJ010000000
VERSION     DAJSKJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.408"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            95..545
                     /locus_tag="ON345_004025"
                     /pseudo
     CDS             95..545
                     /locus_tag="ON345_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          172 a          112 c          124 g          137 t
ORIGIN      
        1 tcgaacgtgt acatacaaat atgcacaaaa ataatcaaaa ttattttctg agatgcatta
       61 tgatatgaac accaatttcg tatagagtct cactatgtct cgaatttttg cttactgtcg
      121 gatatcaacg ctggatcaga ccaccgaaaa tcaacgccgg gaaatcgaaa gtgcaggttt
      181 taaaatcaaa cctcagcaaa taatcgaaga acacattagc ggctcagcag caaccagtga
      241 gcgtcctggt tttaaccggt tgcttgctcg cctgaaatgt ggtgatcaat tgattgtgac
      301 aaaactggat cgccttggtt gtaatgcaat ggatatcagg aaaacagtgg aacaactgac
      361 cgaaacaggt atcagagtgc attgcttagc attggggggc attgacctga ccagtccaac
      421 aggaaaaatg atgatgcaag taatttcagc agtcgctgaa tttgaacgag accttttact
      481 tgaacgcact cattccggga tagtaagagc ccgggcgcag ggaaacgttt tggtcgacca
      541 cctgt
//
LOCUS       DAJSKJ010000403          544 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000403 DAJSKJ010000000
VERSION     DAJSKJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.409"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..399)
                     /locus_tag="ON345_004026"
     CDS             complement(<1..399)
                     /locus_tag="ON345_004026"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="YdiB; quinate/shikimate dehydrogenase from
                     Escherichia coli uses both NAD and NAD(P) to convert
                     quinate and shikimate to 3-dehydroquinate and
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR3073979.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKVLEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLAGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAG"
     gene            complement(411..>544)
                     /locus_tag="ON345_004027"
     CDS             complement(411..>544)
                     /locus_tag="ON345_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3073980.1"
                     /translation="LLALITAIIVFIRYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
BASE COUNT          141 a          132 c          113 g          158 t
ORIGIN      
        1 tccggcccct aacagcacca ttgttttgcc tttgatatca aaaccgctct ctttaatggc
       61 gcgaatatgg cccgtgccgt cggtgttata gccgcgcagg tagccatcat cattaacgat
      121 ggtgttgatg gcacccgcca gtttggctgc tggcgttaat tcatcaacat attcacacgc
      181 cagttgtttg ttcggcattg atataccagt tccgcgcatt ttgagggctt ttaatccttc
      241 aattgctccg ggaaaactat cgttatccac ttcaaaggcc atataggtaa atggcaatcc
      301 cgctttttct aagactttat tctgcatttc aggcgataaa ctgtggcgga taggataggc
      361 catcagccca atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta
      421 tgcttaattg tgtttaaccg ggtgctaaat ttgcgctcgc caaaccgcac atcattctca
      481 ggaataataa aaacgcggta atagcggata aaaacaataa ttgcggtaat cagggccagc
      541 agcg
//
LOCUS       DAJSKJ010000404          540 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000404 DAJSKJ010000000
VERSION     DAJSKJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.410"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            62..540
                     /locus_tag="ON345_004028"
                     /pseudo
     CDS             62..540
                     /locus_tag="ON345_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151472.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          184 a          111 c          101 g          144 t
ORIGIN      
        1 actatttacc gtaaaatatc cccatcacac agtaacctga tacagatacg tggaatggtc
       61 aatgaacaaa ctttcgcctg aaatgcccga actgcaatca atgaatatca ctgccgataa
      121 catcaccaag cttaaatcat tgtttcctga agcctttaat gaaaacggca ttgattttga
      181 cgttttaaaa cagcttttgg gtgaaaatgt agacgaaaaa gaagaacgct atggcctgag
      241 ctggcatggt aagcgtcagg ctcgtcagct tgctcttacc ccttcccgtg gcaccttacg
      301 cccatgtaaa gatgaaagtg ttgattggca taacaccaaa aacctcatga tcgaaggtga
      361 taaccttgaa gtgttaaagc ttcttcaaaa aagttatgcc ggaaaagttt agcttattta
      421 tattgatccg ccttacaata ccggtaaaga ttttgtttat tctgataact tccaagataa
      481 catgaaaaat tatcttgaaa tgacaggaca aacagaagat ggggtacgaa tcaccacaaa
//
LOCUS       DAJSKJ010000405          538 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000405 DAJSKJ010000000
VERSION     DAJSKJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.411"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..538
                     /locus_tag="ON345_004029"
                     /pseudo
     CDS             1..538
                     /locus_tag="ON345_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    80..196
                     /locus_tag="ON345_004029"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          125 a          133 c          161 g          119 t
ORIGIN      
        1 ctgaacgtca gcgtctgaaa gagctggaac gtgaaaatcg tgaactgcgc cgcagtaacg
       61 atatccttcg ccaggcttct gcttattttg cgaaggcgga gttcaaccgc ctctggaaaa
      121 aataatgcca ctgctggata agctgcgtga gcagtacagg gtcggaccgg tatgcagtga
      181 actgcatatt gccccgtcaa cgtattacca ctgtcagcaa cagcggcatc atcctgataa
      241 acgcagcgcc cgtgcgcagc gcggtgactg gctgaagaaa gagatactgc gcgtatacga
      301 tgagaatcat caggtatacg gtgtgcgtaa agtctggcgt cagtttttac gggaaggtat
      361 cagagtggtc aggtgctctg tggcacgtct tatggcggtt atgggtcttg ccggtgtcct
      421 ccggggtaaa aaggtccgca cgaccgtcag ccggaaagcc gttgccgcag gcgaccgcgt
      481 aaaccgtcag ctcgtggcag aacgtcctga ccagctgtgg gtggctgatt ttacttac
//
LOCUS       DAJSKJ010000406          537 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000406 DAJSKJ010000000
VERSION     DAJSKJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.412"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>537)
                     /locus_tag="ON345_004030"
     CDS             complement(<1..>537)
                     /locus_tag="ON345_004030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073981.1"
                     /translation="LLKSQIANNDLGWRAILPAVLVSSSLIAISIEKLKREAITCISL
                     IVVAISLPSAIDFSRNLLQGDFRNDNVKIISSLLDITNNTEPTDRILINPRKFEGRDV
                     LDGNVLPSIIMDRNSCFTNLSYAYAFSNQWGESIGYYWNALKEFYAGHASNEDLAKMK
                     KLKCTKLVILPGDGIWDK"
BASE COUNT          172 a          108 c           84 g          173 t
ORIGIN      
        1 aatttatccc aaatgccgtc tccaggtaaa atgactaact tagtgcactt cagctttttc
       61 atctttgcta ggtcttcatt cgatgcatgg ccggcataaa attcttttaa tgcattccag
      121 taataaccaa tggactcacc ccactggtta gaaaaagcat atgcataaga cagattcgta
      181 aagcaactat ttctgtccat tattatagat ggaagaacgt tcccatctaa aacatcacgt
      241 ccttcaaatt ttctaggatt tatcagtatt cgatcagtag gctctgtatt attagttata
      301 tccaataagc ttgaaataat cttgacattg tcattgcgga aatcaccttg caataaattt
      361 cttgaaaaat ctattgcgga tggcaaacta atggctacaa caatgaggct tatgcatgta
      421 atagcttccc ttttcaattt ttcaattgat attgctatta gtgaagatga aacaagaaca
      481 gcaggcagaa tggctctcca tcccaagtca ttattagcaa tctgactttt caataac
//
LOCUS       DAJSKJ010000407          536 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000407 DAJSKJ010000000
VERSION     DAJSKJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.413"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..285
                     /locus_tag="ON345_004031"
     CDS             <1..285
                     /locus_tag="ON345_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR3073982.1"
                     /translation="GSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNE
                     WIPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKKL"
BASE COUNT          144 a          111 c          153 g          128 t
ORIGIN      
        1 ggcagtcatt atacgagcag gcagtttcgg cagttattgt ggcgatacca gatcaggcag
       61 agtatgagcc ggcgcggaaa ttgctgggat aacagcccaa tggaacgctt cttcagaagt
      121 ctgaagaacg aatggatacc ggtggtgggt tacgtaagct tcagcgaggc agctcacgcc
      181 ataacggatt atatcgttgg atattacagc gcactaagac cgcacgaata taacggtggg
      241 ttacccccaa acgaatcgga aaatcgatac tggaaaaaac tctaactcgg tggccagttt
      301 ttgttgacca cttcaaccct gccgggctaa cagagaggtt agacatgaag tgaggtgcga
      361 tgctggcagg catcaggtga ggggctgata ccacacttct gccatggttt ctgaattatc
      421 aatgtccgct tctgggcata gacgagattc atgctcggca ggggccgtag caagcgaaaa
      481 gcggaggttc gggaaggttg tgtttgcttt taacctttgt aatttagaag tattat
//
LOCUS       DAJSKJ010000408          527 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000408 DAJSKJ010000000
VERSION     DAJSKJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.414"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>527
                     /gene="tuf"
                     /locus_tag="ON345_004032"
     CDS             <1..>527
                     /gene="tuf"
                     /locus_tag="ON345_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3073983.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGR"
BASE COUNT          128 a          130 c          146 g          123 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggccgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccg
//
LOCUS       DAJSKJ010000409          514 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000409 DAJSKJ010000000
VERSION     DAJSKJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.415"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>514
                     /locus_tag="ON345_004033"
     CDS             <1..>514
                     /locus_tag="ON345_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3073984.1"
                     /translation="ASGDAIIPIDVDLQDPIDIIPEMIEKWLNGADVVLAKRIDRSSD
                     GRLKRKTAEWFYKLHNVISSPKIEENVGDFRLMSREIVENIKKLPEKNLFMKGVLSWV
                     GGKTEIVEYVRAERIIGQTKFNGWKLWNLAIEGITSFSTFPLRMWTYIGAFVAGMAFI
                     YGVYMILSTLI"
BASE COUNT          168 a           84 c          113 g          149 t
ORIGIN      
        1 gcttcagggg atgctatcat tccgattgat gtagatttac aggatcccat cgatatcata
       61 ccggaaatga ttgaaaaatg gctcaatggc gccgatgtcg tactagccaa gcgcatagat
      121 agatcctctg acgggagatt aaaacgtaaa acagccgaat ggttctataa actacataat
      181 gttatcagtt cacctaaaat agaagaaaat gtcggagact ttagattaat gtctcgggaa
      241 atagttgaaa atatcaagaa gcttcccgaa aaaaatctat ttatgaaggg ggttctttca
      301 tgggtaggag gaaaaacaga aatcgttgaa tatgtacgtg cagaacgtat aatcggccaa
      361 acaaaattca atggttggaa attatggaat ctcgctattg agggaataac ttctttttca
      421 acgtttccgt tgcgtatgtg gacttatatc ggtgcatttg ttgccggaat ggcattcatc
      481 tacggcgttt atatgatatt aagcacatta attt
//
LOCUS       DAJSKJ010000410          512 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000410 DAJSKJ010000000
VERSION     DAJSKJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.416"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            1..259
                     /locus_tag="ON345_004034"
                     /pseudo
     CDS             1..259
                     /locus_tag="ON345_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424705.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            248..512
                     /locus_tag="ON345_004035"
                     /pseudo
     CDS             248..512
                     /locus_tag="ON345_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
BASE COUNT          149 a          105 c          124 g          134 t
ORIGIN      
        1 gatatttata gtagcgattt atttgattta tattcttttt ttcaaaaaga agaaaactaa
       61 gtccgcgggc tcttatactt ccgctcctgt gaaagcacca cctaaagaat ggcttgctaa
      121 taacaaaaaa aatcagtctg gaatgcacaa cgaggacagc gaagatgaca atctggctac
      181 gtttacacta agtggcgggc ggaatgccga atacagtata acagccactc aacgccagaa
      241 tttctcaaaa actaccggta tcagcacctg ggattacggg tacggataca gtggtcgtga
      301 atcgttgtca cgctcaggct ctgctgacaa cttcgcatgg agccttgggg ccggtgttcg
      361 ttatgacgta accccggatg tcgctctgga cctcagctat cgctatcttg atgcaggtga
      421 cagcagtgtg agttacaggg acgagtgggg cgataaatat aaatcagaag ttgatgttaa
      481 aagtcatgat atcatgcttg gtgtgactta ta
//
LOCUS       DAJSKJ010000411          502 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000411 DAJSKJ010000000
VERSION     DAJSKJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.418"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>502
                     /locus_tag="ON345_004036"
     CDS             <1..>502
                     /locus_tag="ON345_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCR3073985.1"
                     /translation="RRDGTLPMTGDLNLDGHNINNVAALNATGNITTTGDVQARDIKA
                     TGKIDADGNISAGNWMWAKNGYGDAIGFGGDGYSGGLGRDYEIKMLSNHPLTIHSPTS
                     SRGNDVILDIDGNMRVQTDISSLRNITASGNIEAAKNVKGATLESTGRATVGEFVQVN
                     GTVSEG"
BASE COUNT          162 a           83 c          131 g          126 t
ORIGIN      
        1 taagacgtga tggaacattg ccaatgactg gcgatttaaa cttagatggg cataatatta
       61 acaatgtcgc cgcacttaat gccactggca acattacgac gacaggtgac gtacaggcaa
      121 gagacattaa ggctactgga aaaattgatg cggacggtaa tatttcagcc ggtaattgga
      181 tgtgggctaa gaatggatat ggggacgcta taggttttgg tggagatgga tattctggag
      241 gtttaggtcg agattatgaa ataaaaatgc tatctaacca tccactaacg atacattcac
      301 caacgtctag tcgcggaaat gatgttatat tagatattga tggaaatatg agggttcaga
      361 ctgatattag ctctcttaga aacattacag cctctggaaa catagaggcc gccaaaaatg
      421 tcaaaggcgc tacattagaa tccacaggac gcgctacggt gggtgaattt gttcaggtta
      481 atggaacagt aagcgagggg ga
//
LOCUS       DAJSKJ010000412          495 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000412 DAJSKJ010000000
VERSION     DAJSKJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.419"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>495)
                     /locus_tag="ON345_004037"
     CDS             complement(<1..>495)
                     /locus_tag="ON345_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR3073986.1"
                     /translation="ANYDTWGYHDNTTTDLVWPYDTDNDGIADAGGMDPMGSDGNYVT
                     YNGFVYYNNANGDFDTVFKGDTVNGTISTYYLNHDYASGSVNQVDISNSVIHGSITSE
                     LPFGYYSITPEGYNGYTFNNGTTTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTH
                     LDGD"
BASE COUNT          149 a          100 c          106 g          140 t
ORIGIN      
        1 tatcaccatc gagatgtgtg tcagtgaagt aaagagcttc atagtcatca tcaatggtgg
       61 aattggatac gttcagagtg aatacgtcac catcgtacca attgtagtca agatcagtag
      121 tagttccgtt attgaaagta tagccattat aaccttcagg cgtaatgctg taataaccaa
      181 atggcagttc agaagtgatt gacccatgaa ttactgaatt gctgatatct acctggttaa
      241 cagaaccgct agcgtaatca tgattcaagt aataagtaga aatggtaccg ttaacggtat
      301 cgcctttaaa gacggtatcg aaatcgccat tggcattgtt atagtaaaca aaaccattgt
      361 aagttacgta gttaccatct gaacccatcg ggtccatgcc gccagcatcg gcaattccat
      421 cattgtcggt gtcatacggc caaactaaat cggtcgtggt gttgtcatga tacccccagg
      481 tatcatagtt tgctg
//
LOCUS       DAJSKJ010000413          492 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000413 DAJSKJ010000000
VERSION     DAJSKJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.420"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT          164 a           95 c           78 g          155 t
ORIGIN      
        1 ctctctgaaa ttttcttgca gtaactgcac tcgcagaaat agagaaaaaa aaatacaatt
       61 gttaataacc ttgccatatt ttttagtccg taactgttaa tgtgctatct ggctctgaaa
      121 atattaactc atttaatttt tgcatgtcat tagataaacc agttatacaa ccctcggaac
      181 agaatcgagg tcctaaagac ggaccaaaag cgtttaaaca actatgtatt gaaaatccat
      241 tgcgattata agttcctgta ttaactgacg gtactaatcg tatgccgtca ggttttctat
      301 tgcttgctcc cgggtcatta acattccaac tccataggcc ttgaggtatt ggccatctat
      361 ttataaatgt gtacaacggg gattattttt acattgcata ttattaccag aagcgactgg
      421 aatactaagc acatcataaa aattatcatc acatggtata tctgaaacac acgaaagcat
      481 acctaaagtt at
//
LOCUS       DAJSKJ010000414          486 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000414 DAJSKJ010000000
VERSION     DAJSKJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.421"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(11..178)
                     /locus_tag="ON345_004038"
     CDS             complement(11..178)
                     /locus_tag="ON345_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3073987.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKWECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(181..345)
                     /locus_tag="ON345_004039"
     CDS             complement(181..345)
                     /locus_tag="ON345_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR3073988.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT          146 a          110 c           94 g          136 t
ORIGIN      
        1 atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata aaggcttgct
       61 gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccat ttcgtttctg
      121 tttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga ccgaccatag
      181 ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg cagaaccaag
      241 aaaaaccgcc agccaggctt tacgttttaa ttcaggatcg cggcggggtt cctgatttcc
      301 agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt tgaacgattg
      361 gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa atccagcgaa
      421 tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat atttgaaaac
      481 tggagt
//
LOCUS       DAJSKJ010000415          476 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000415 DAJSKJ010000000
VERSION     DAJSKJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.422"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>476
                     /locus_tag="ON345_004040"
     CDS             <1..>476
                     /locus_tag="ON345_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3073989.1"
                     /translation="RSTLYYQLSLQKAKDKYADVKQLIASIFHEHRGCYGYRRIHCEL
                     QKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAENILQRQFKAEAPCEKW
                     VTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVKRMLNKGLESLAEGE"
BASE COUNT          136 a          106 c          136 g           98 t
ORIGIN      
        1 ccagaagcac gctgtactac cagctgtcac tgcagaaggc taaggacaag tatgctgatg
       61 tgaaacagct catagcctcc atcttccatg aacaccgggg atgttatggg taccggcgga
      121 tccattgtga gcttcagaaa agaggtctta agttcagtgg caaaacggtg cgaaaactga
      181 tgcaacagct cggcctgaag tctcctgtgc gcctgaagaa ataccggtct taccggggaa
      241 atatgggact cgctgcagaa aatatccttc agcgacagtt caaagcggaa gctccctgtg
      301 agaaatgggt gacagatatc accgaattca gggctggcgg acaaaaactg tacctgtccc
      361 cgatacttga tttgtttaac ggggaaatcg tggcgtggga aactgcctgc aggcctacag
      421 aggagctggt gaagcgcatg ttaaataagg ggctggaaag ccttgcagaa ggagaa
//
LOCUS       DAJSKJ010000416          473 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000416 DAJSKJ010000000
VERSION     DAJSKJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.423"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..470)
                     /locus_tag="ON345_004041"
     CDS             complement(<1..470)
                     /locus_tag="ON345_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR3073990.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGV"
BASE COUNT          122 a          132 c           82 g          137 t
ORIGIN      
        1 acacctttat caagatattc cttcgctttc ttacaatcta cctcaacata ctgaccattg
       61 gcgtacataa tgcccaacct ggaccatgct aaattcacac cgtgctgcgc ggcctgatta
      121 aatagtgcat acgctttggc ataatccacc cgcacctgct caccgtcgta gtagataccc
      181 cccagcgcta acggagcttc tctgtcacct tgtttagccg ctaactcgaa ccatttaatc
      241 gcttcctgaa catcaggttt ggtatcataa cgaggctgat aatattccca gcctaacagg
      301 ctctgcgcgt cgatatctcc cgattcagcc attttatgca gtgtatttat aacatcggga
      361 gcaggtttac caccgtatat gctgtcatac aattcatgcg ctttctcgct ggccaccacg
      421 cttggtgaaa ctatcagcat tacagaaaat aatattcttt tagttaacat gta
//
LOCUS       DAJSKJ010000417          467 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000417 DAJSKJ010000000
VERSION     DAJSKJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.424"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            137..262
                     /gene="blr"
                     /locus_tag="ON345_004042"
     CDS             137..262
                     /gene="blr"
                     /locus_tag="ON345_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR3073991.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT          131 a           94 c           96 g          146 t
ORIGIN      
        1 aaaaaaatag cgaattttcc aacgacaaaa gctaaatatc gcaaaaacct cagtaaaaat
       61 cttgctggag ctattattgc taagtaacat ttaccccctg aagttaatgg atcaatcaag
      121 agagatgtgg gctgtaatga atcgtcttat tgaattaaca ggttggatcg ttcttgtcgt
      181 ttcagtcatt cttcttggcg tggcgagtca cattgacaac tatcagccac ctgaacagag
      241 tgcttcggta caacacaagt aagctctgcg cttgtagagc gccatgctgc ccgtccgggt
      301 gcatgttttc acttgtcgga tattaaacca ggaatttatt atcttgttcg atgttgttgg
      361 tgattgtcag ggatagtaaa gcgcgttact cttttcagag cagtttttca tccctgaata
      421 tttgccatca gcgcattctt attattgtaa taacacaagc gttcact
//
LOCUS       DAJSKJ010000418          463 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000418 DAJSKJ010000000
VERSION     DAJSKJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.425"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            64..407
                     /locus_tag="ON345_004043"
                     /pseudo
     CDS             64..407
                     /locus_tag="ON345_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          116 a          109 c          133 g          105 t
ORIGIN      
        1 tgagtcattt aagatggctc agaggcgtcc acgagcggcc agcgttatcc cgaagagttt
       61 caaattgaat cggtaaacaa gttgttgacc gtggccattc tgtttccagc gtgtgcaaca
      121 cgccttgata tcaccacgca cagtctttat gcctagctcc ggcagtcgca ttctcagcct
      181 caccaagaag acgagatgct gaccgggcag attaaacaat tctggctgga gtctggttgt
      241 gtttatggct atcgcaagat ccatctggag ctgcgcgata gcgggcaaga gtgcggagtg
      301 aacagagtct gcgactgatg aagcgtgtcg ggataaaggc tcaggtcgga taccggagcc
      361 cgcgagcacg caaaggtgaa gctagtatag tgtcgcccaa caagctctag cgatagttca
      421 atctggatac tccgggtgag cgttgggtaa cggacataac tta
//
LOCUS       DAJSKJ010000419          443 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000419 DAJSKJ010000000
VERSION     DAJSKJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.426"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>443
                     /locus_tag="ON345_004044"
     CDS             <1..>443
                     /locus_tag="ON345_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HCR3073992.1"
                     /translation="AIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYN
                     RLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQI
                     LNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDG"
BASE COUNT          167 a           80 c           78 g          118 t
ORIGIN      
        1 ggctattcat caccagatca ttgaaaacca actggataca gaaacaataa ctattagtta
       61 tgaatttatc ctctgtcaac atcttatcgc aaataccgag ttcagctatt tggcactccc
      121 tgaaaattat aaccgtttgt ttttaccaaa ttcaaaaaac caaacaaata atcgtttcaa
      181 aacgttaaac agcaaagcta ttggcaggct acttgctgct ggtggcgtat acaatgggaa
      241 cattgaagga ttcagagata ctgcggaaaa actgggtgga gatgcaataa aaggctatga
      301 tcaaatacta aatgaaaaaa cagcgggcat agcgatagca acagcatcta ttcttttaac
      361 aaagcgttct aatgttgata catatacaga aataaatagt tacttaggca aacttagagg
      421 tcaacaaaaa cttcttgatg gta
//
LOCUS       DAJSKJ010000420          439 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000420 DAJSKJ010000000
VERSION     DAJSKJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.428"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..229
                     /locus_tag="ON345_004045"
     CDS             <1..229
                     /locus_tag="ON345_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR3073993.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQCEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          143 a           92 c           81 g          123 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtgcgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcgtatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccactt
//
LOCUS       DAJSKJ010000421          433 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000421 DAJSKJ010000000
VERSION     DAJSKJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.429"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            44..>433
                     /locus_tag="ON345_004046"
     CDS             44..>433
                     /locus_tag="ON345_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3073994.1"
                     /translation="MVDKVTKAAVVGGVDTHKDLHVAAVVDQNNKVLGTQYFSTTRQG
                     YRQMLAWMTSFGTLKRIGVECTGTYGSGLLRYFQNAGLEVLEVTAPDRMERRKRGKSD
                     TIDAECAAHAAFSGIRTVTPKTRDGMIE"
BASE COUNT          108 a           93 c          118 g          114 t
ORIGIN      
        1 tctgtctgga gattcaactc tgtttcctca caggagctct gtcatggttg ataaagttac
       61 caaagctgcc gttgtgggtg gcgtggatac acataaagat ttgcacgttg ccgctgtcgt
      121 agatcagaac aataaagtcc ttgggactca gtatttctcc acaacacgac aaggttaccg
      181 gcagatgctg gcatggatga cctcgtttgg gacattaaag cgaattggtg ttgagtgcac
      241 aggtacctat ggttccggtt tgcttcgtta ttttcagaac gccgggttag aagttcttga
      301 ggtgactgct ccagaccgga tggagcgacg caaacggggt aaaagtgaca caattgatgc
      361 tgaatgtgcc gctcatgcag cattctcagg catcaggaca gttactccca aaacgcgtga
      421 tggcatgatt gag
//
LOCUS       DAJSKJ010000422          424 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000422 DAJSKJ010000000
VERSION     DAJSKJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.430"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT          124 a           72 c           82 g          146 t
ORIGIN      
        1 ccctaagttg tcgttgctta ttccaattcg ttactataaa tatggcaaaa atattacaac
       61 aaacagcggt taaaaaattt ggtgaagatt ttttgtaata atcctaatta gttatgggaa
      121 tttcgcttga tgtatctgca taacaaaaac aatatgcgcc atttttgcat tactaataag
      181 aaaaagcaaa aaacatcgat attcattttt ggtcaatggg ttttctggcc tatcgttgtt
      241 attgtgctac tggttatcat tttcatcttg ccaggatcgc acgtaatcac gttattcggg
      301 gactttatct gcgggatatt acccttcagg taagtccttt tctcgccagc atattggagc
      361 gtgatcaata ttgatcagct gtgaaggggg tgatgtgtag tggaccaaaa cttacgttat
      421 atca
//
LOCUS       DAJSKJ010000423          423 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000423 DAJSKJ010000000
VERSION     DAJSKJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.431"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            118..>423
                     /locus_tag="ON345_004047"
     CDS             118..>423
                     /locus_tag="ON345_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3073995.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     "
BASE COUNT          126 a           84 c           85 g          128 t
ORIGIN      
        1 cgttatcctg gggagaaaac gcctcaacaa aatgcatctt caattattca ctttcgacac
       61 cagaaagtga gagcggatta attgaacagg gtgtgacatg tcattaaaag gatttttatg
      121 aacaagtata tcaaacagtg gtgctttgct gtgtttatgc tctcgttaag tagtgtagca
      181 cttgcagctc ctaaaggtat ctgtacccct gataatggcg tatttcacag tacgcttgat
      241 ttctctggat atcttattac cgcgaacgaa aataaggttg gaacgacatt taacaccact
      301 gtaactaatg gatcctcata tcctggacgc tgtcattgtg atactggtaa cgtaggagaa
      361 ttcccctata tctattatac gtcaaaaata aaccaggcat taacttatgc tggggttcac
      421 tct
//
LOCUS       DAJSKJ010000424          418 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000424 DAJSKJ010000000
VERSION     DAJSKJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.432"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            88..279
                     /locus_tag="ON345_004048"
     CDS             88..279
                     /locus_tag="ON345_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR3073996.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          101 a          108 c           81 g          128 t
ORIGIN      
        1 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
       61 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
      121 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
      181 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
      241 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc tccactttca
      301 cagaacctaa cggcacttcg ctgtcggatg cttttgctct ttggaattat caaagcggca
      361 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttgg
//
LOCUS       DAJSKJ010000425          411 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000425 DAJSKJ010000000
VERSION     DAJSKJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.433"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>411)
                     /locus_tag="ON345_004049"
     CDS             complement(<1..>411)
                     /locus_tag="ON345_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3073997.1"
                     /translation="ASSATNSLFSFLANAQIIKKTVFPRSVIPLSNVMMEGLHFLCTI
                     PVIAMFLFIYGMSPSIVWLWGIPIIAIGQIIITFGFSLIFSTLNLFFRDLERFVTLGI
                     MLLFYCTPILYGADMIPEQYKWIIDYNPLANMIL"
BASE COUNT          162 a           70 c           71 g          108 t
ORIGIN      
        1 caaggatcat atttgctaaa ggattataat ctataatcca tttgtattgt tccggtatca
       61 tatcagctcc atacaaaata ggagtgcaat agaagagtag cattatccct aatgttacaa
      121 aacgttcaag atccctaaag aaaagattca atgttgaaaa aatcaaagaa aaaccgaagg
      181 ttatgattat ctgaccaatt gcgataattg gaatacccca caaccaaacg atagatggag
      241 acatgccata aataaataga aacatcgcaa tcacagggat cgtgcataaa aaatgcaatc
      301 cttccatcat tacattactt aatggaataa cagagcgtgg aaagactgtt ttcttaatta
      361 tttgagcatt tgcaagaaat gaaaacagtg agttggtcgc agaacttgca a
//
LOCUS       DAJSKJ010000426          410 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000426 DAJSKJ010000000
VERSION     DAJSKJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.434"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>410
                     /locus_tag="ON345_004050"
     CDS             <1..>410
                     /locus_tag="ON345_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR3073998.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQ
                     GPTGEERTVYGIYGISESNLAECEKGVKSAVALT"
BASE COUNT          120 a           96 c          101 g           93 t
ORIGIN      
        1 tgaaaaggaa tttattatct tccgctatta tagtcgccat catggccctc ggtctgacgg
       61 gttgtgatga taaaaaagcc gaaacagaaa cgcccccgcc tgccaatagt caacctgccg
      121 caccagctcc tgaagctccc gttgcaaaag cagaagctaa acctgaaaca cctgcgcaac
      181 cggtggtcga tgaacaagcg gttttcgacg aaaaaatgga tgtctatatc aagtgctata
      241 acaagttaca gatccctgta cagagcagtc tggcgcgtta tgctgactgg ctgaaagatt
      301 ttaaacaggg gcctaccggt gaagagcgta ctgtttatgg catctacggc attagtgaat
      361 ccaacctcgc tgagtgtgaa aaaggcgtaa aaagtgctgt ggcgttaacg
//
LOCUS       DAJSKJ010000427          409 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000427 DAJSKJ010000000
VERSION     DAJSKJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.435"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>409
                     /locus_tag="ON345_004051"
     CDS             <1..>409
                     /locus_tag="ON345_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR3073999.1"
                     /translation="LYNLAHTATNSHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIP
                     LISEKQSLSKVLLNDENNELSDGTNFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPT
                     GLYVPYSTRTHLEDALNENIKSDPSWPNEVQLFP"
BASE COUNT          137 a           96 c           64 g          112 t
ORIGIN      
        1 aattatataa tttggcacat accgcaacca actcccacct taaaccaact ggtccggata
       61 attcagatgt tctcctgttc aaaaagctat tctccccttc gaaattaatg acaattatcg
      121 gtgatgaaat tcctcttata tctgaaaaac agtcgctttc aaaggtgctt ttaaatgatg
      181 agaataatga actgagtgat ggtacaaact tctgggataa aaatcgtcaa ttaaccacag
      241 atgaaatagc ttgctatctt cagaagatcg ccgctaatgc aaaaaatact caagtcaatt
      301 atcctactgg tctctacgtc ccctactcca ccagaactca cctggaagac gctctcaatg
      361 aaaatattaa gagcgatcca tcatggccga atgaagtcca gttattccc
//
LOCUS       DAJSKJ010000428          405 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000428 DAJSKJ010000000
VERSION     DAJSKJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.436"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT          118 a           81 c           78 g          128 t
ORIGIN      
        1 ttcagtattt cagaagttgt gggtaattgt aaagctggcc ttacgcattg ctaaaccagc
       61 gggagtgcaa tttttccttc tggtagcctc tgaaatctgt tttaccgtta tctccgggtt
      121 gcatacacat cacgaaaaca ggtaattagt agcttacttc tttacttatc atagtcgtca
      181 tattttggac gatacggctg ctgataatgt actgcgaaaa aatgtgttct gtgaacgata
      241 aataccacca ttgacttttt attgttcccg taccagcgtc cgggggggcg ttcagatgat
      301 attgttcagc atattaaacc actcatatat caaaagacag gaaatatatt ttcgcccaca
      361 gccgaatcag atttaaaata aagcaacatt gttttcaaac actga
//
LOCUS       DAJSKJ010000429          392 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000429 DAJSKJ010000000
VERSION     DAJSKJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.437"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>392
                     /locus_tag="ON345_004052"
     CDS             <1..>392
                     /locus_tag="ON345_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR3074000.1"
                     /translation="HTTKLTSILVMKPCKDILEQLIANGALTPYVRTTGLIENNFQIC
                     SSVSGFKKMNVNDVYGTSFHNKNKESRIVSISGTSFVPGKTAIVFLMPIGNDMTAFSI
                     VESRYIYDLMDVLDDENDDSFSLRFTEGP"
BASE COUNT          125 a           65 c           77 g          125 t
ORIGIN      
        1 catacgacaa agcttacctc tattctggtg atgaagccct gcaaagacat tcttgaacag
       61 ttgattgcta acggagcatt gactccctat gttcgaacaa cgggtcttat agaaaataat
      121 ttccaaatat gttcttcagt tagcggtttc aaaaaaatga atgttaacga tgtttacggt
      181 acgagctttc acaataaaaa taaagaatca cgtatagtat caattagcgg tacaagtttt
      241 gtgccaggta aaacagcaat agtgttcctt atgccaatag ggaatgatat gacagctttt
      301 agtattgtag agtcacgata tatctatgat ctaatggatg ttcttgatga tgagaatgat
      361 gattcatttt ctcttcgttt tactgaaggg cc
//
LOCUS       DAJSKJ010000430          392 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000430 DAJSKJ010000000
VERSION     DAJSKJ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.438"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            103..378
                     /locus_tag="ON345_004053"
     CDS             103..378
                     /locus_tag="ON345_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3074001.1"
                     /translation="MPTVLSRMAMQLKKTARIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT           93 a           95 c           77 g          127 t
ORIGIN      
        1 taaagctatt tttattcatc aagaaaatag ccatactgac gccttcaatg gatgttgata
       61 ttcactacag atattgttcg cagcagacag gatgtattta atatgccaac agttctctct
      121 cgcatggcga tgcaactcaa aaaaacagcc cggataattc ccgtcttcat ggtttcggga
      181 tgctcattat ctccggcaat cccggtgatc ggcgcttatt atcccagctg gtttttctgc
      241 gccattgcca gccttatttt gacgctcatc acgaggcgag ttattcagcg ggcaaatatc
      301 aatctggcat ttgtcggaat tatttatacc gccctttttg ctctctacgc catgctgttc
      361 tggctggcat ttttctaatc tatttgagac gc
//
LOCUS       DAJSKJ010000431          386 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000431 DAJSKJ010000000
VERSION     DAJSKJ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.439"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..370
                     /locus_tag="ON345_004054"
     CDS             <1..370
                     /locus_tag="ON345_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3074002.1"
                     /translation="ESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADN
                     NIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIA
                     DWPINRVGELLPWRVALPTE"
BASE COUNT           95 a          103 c          117 g           71 t
ORIGIN      
        1 ggaaagctgg ctgcgtgaaa agatgaaaac cctgtcgcga cactcagaac tggcgaaagc
       61 gttcgcatac gccctgaacc agtggccggc gctgacgtac tatgcagatg atggctgggc
      121 tgaggcggac aataacatcg ctgaaaatgc gttgcggatg gtcagtctgg gccgcaaaaa
      181 ctacctgttc ttcggttcgg atcatggagg agagcgggga gcgctgctgt acagcctgat
      241 cgggacgtgc aaactgaacg gagtggagcc agaaagctac ctccgctatg tccttgacgt
      301 catagccgac tggccgataa accgggtcgg cgaactgctc ccctggcgcg tagcactgcc
      361 gactgaataa cacatccccg tcaata
//
LOCUS       DAJSKJ010000432          383 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000432 DAJSKJ010000000
VERSION     DAJSKJ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.440"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>383
                     /locus_tag="ON345_004055"
     rRNA            <1..>383
                     /locus_tag="ON345_004055"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          101 a           90 c          116 g           76 t
ORIGIN      
        1 ggagactgcc agtgataaac tggaggaagg tggggatgac gtcaagtcat catggccctt
       61 acgaccaggg ctacacacgt gctacaatgg cgcatacaaa gagaagcgac ctcgcgagag
      121 caagcggacc tcataaagtg cgtcgtagtc cggattggag tctgcaactc gactccatga
      181 agtcggaatc gctagtaatc gtggatcaga atgccacggt gaatacgttc ccgggccttg
      241 tacacaccgc ccgtcacacc atgggagtgg gttgcaaaag aagtaggtag cttaaccttc
      301 gggagggcgc ttaccacttt gtgattcatg actggggtga agtcgtaaca aggtaaccgt
      361 aggggaacct gcggttggat cac
//
LOCUS       DAJSKJ010000433          380 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000433 DAJSKJ010000000
VERSION     DAJSKJ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.441"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>380
                     /locus_tag="ON345_004056"
     CDS             <1..>380
                     /locus_tag="ON345_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3074003.1"
                     /translation="LIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDAKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRRINSIPITA"
BASE COUNT          114 a           93 c           90 g           83 t
ORIGIN      
        1 agctgattaa acgtaatgct atcggatacg aaagcgcttc acagttgctg atcactgccg
       61 gagacaatcc tcaacggtta agatcagaat caggatttgc ggcactgtgt ggtgtcagcc
      121 cggtccccgt ttcttctgga aaaacgaacc gttaccgact taatcgtggt ggagatcgtg
      181 ctgcaaatag tgcacttcac atcatcgcca tcggacgctt acgaactgac gcaaaaacaa
      241 aggaatatgt cgccaggcgt gtagccgaag gacatacaaa aatggaagcg atacgctgtc
      301 taaagcgata tatctcccgc gaagtttaca cgttactgcg caatcaaaac aggcggatca
      361 acagtatccc gataacagct
//
LOCUS       DAJSKJ010000434          380 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000434 DAJSKJ010000000
VERSION     DAJSKJ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.442"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(116..271)
                     /locus_tag="ON345_004057"
     CDS             complement(116..271)
                     /locus_tag="ON345_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3074004.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT          120 a           57 c           62 g          141 t
ORIGIN      
        1 atattattaa atgctggttt tgaaaaaagt tcatcggata agatatagtc acgaaataag
       61 tatctgctaa tattttatga tttctttttc agaacgtcga acgggatttt cttatttaag
      121 atatcagaat cttatgattc gggtaaaatt ctaaataaat caacatgtca ttaaaatcat
      181 catagccatt agctatttcg gctaaaatag agactacatg tcttcggtcc atctcactta
      241 aggagtgtag ttccgttgta agtttttcca tagcttgcac tgctaaattt cgaacaagga
      301 attttctgct ggtaatctct aaaaagatgg catggtttac aatgattttt gtttcctttt
      361 gattattatg aacaactgtc
//
LOCUS       DAJSKJ010000435          369 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000435 DAJSKJ010000000
VERSION     DAJSKJ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.443"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..242)
                     /locus_tag="ON345_004058"
     CDS             complement(<1..242)
                     /locus_tag="ON345_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR3074005.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          114 a           68 c           76 g          111 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgcgaa
//
LOCUS       DAJSKJ010000436          367 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000436 DAJSKJ010000000
VERSION     DAJSKJ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.444"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            58..>367
                     /locus_tag="ON345_004059"
     CDS             58..>367
                     /locus_tag="ON345_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR3074006.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     S"
BASE COUNT           96 a           73 c           89 g          109 t
ORIGIN      
        1 gaatagtcat tcttcaaacc agtagtctga ctaatggaat tttattcaag gatttttatg
       61 tctaaaaaat taggttttgc cctgtctggt cttatgctgg caatggttgc aggtactgct
      121 tctgctgata tggatggtgg tcagttaaat atcagtggtc tggttgttga taacacctgt
      181 gaaacccgcg ttgacggcgg taataaagat ggtctgatcc tgctgcaaac tgcaactgtt
      241 ggtgaaatcg atgctggcgt gctgaatgac accgttggcg ctaaagccaa acctttcagc
      301 atcaccgttg actgcagcaa agcaaatccg aatccaggta gtacagctaa aatgaccttc
      361 ggttctg
//
LOCUS       DAJSKJ010000437          360 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000437 DAJSKJ010000000
VERSION     DAJSKJ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.445"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>360
                     /locus_tag="ON345_004060"
     CDS             <1..>360
                     /locus_tag="ON345_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3074007.1"
                     /translation="TVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLISKYAE
                     HTPLYRQSEMYGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPV
                     PVLLPGNKKTKTGRLWT"
BASE COUNT           71 a          104 c          115 g           70 t
ORIGIN      
        1 gactgtacgt gaaaagcatg cctgtactca gtgcgatgcc atcgtgcagg cccccgcgcc
       61 ttcacggccc atcgagcggg gtatcgcagg accggggctg ctggcccgcg tgctgatctc
      121 aaagtatgca gagcacaccc cgctgtaccg ccagtctgaa atgtacggcc gccagggcgt
      181 ggagctgagt cgttcactgc tgtcgggctg ggtggatgca tgctgccggc tactgtcacc
      241 gctggaagaa gcgcttcagg actatgtgct gactgacggt aagctccatg ctgatgacac
      301 gcctgtcccg gtgctgttgc caggcaataa gaaaacgaag accgggcggt tatggaccta
//
LOCUS       DAJSKJ010000438          357 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000438 DAJSKJ010000000
VERSION     DAJSKJ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.446"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            6..>357
                     /locus_tag="ON345_004061"
     CDS             6..>357
                     /locus_tag="ON345_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR3074008.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDK"
BASE COUNT          100 a           84 c           91 g           82 t
ORIGIN      
        1 gaatagtgat gaaatttaaa aaatgtcttc tgcctgtggc aatgttagcg tcattcactc
       61 tggcaggatg ccagtcaaat gctgacgatc atgccgccga tgtttatcaa accgatcaac
      121 tgaataccaa acaagaaact aaaaccgtta atattatttc cattcttccc gcaaaagttg
      181 ccgtagacaa ctcccaaaat aaacggaacg cacaagcctt cggcgcgctt attggcgcag
      241 tcgctggcgg tgttatcggc cacaacgtcg gttctggcag caattccgga acgacggcag
      301 gggcagttgg cggcggagct gtaggcgcgg cagcgggttc tatggtgaat gataaaa
//
LOCUS       DAJSKJ010000439          357 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000439 DAJSKJ010000000
VERSION     DAJSKJ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.447"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>357)
                     /locus_tag="ON345_004062"
     rRNA            complement(<1..>357)
                     /locus_tag="ON345_004062"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a          129 c           67 g           84 t
ORIGIN      
        1 tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc ctacacgctt
       61 aaaccgggac aaccgtcgcc cggccaacat agccttctcc gtcccccctt cgcagtaaca
      121 ccaagtacag gaatattaac ctgtttccca tcgactacgc ctttcggcct cgccttaggg
      181 gtcgactcac cctgccccga ttaacgttgg acaggaaccc ttggtcttcc ggcgagcggg
      241 cttttcaccc gctttatcgt tacttatgtc agcattcgca cttctgatac ctccagcatg
      301 cctcacagca caccttcaca ggcttacaga acgctcccct acccaacaac gcatcag
//
LOCUS       DAJSKJ010000440          344 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000440 DAJSKJ010000000
VERSION     DAJSKJ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.448"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="ON345_004063"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="ON345_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3074009.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJSKJ010000441          336 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000441 DAJSKJ010000000
VERSION     DAJSKJ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.450"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..281
                     /locus_tag="ON345_004064"
     CDS             <1..281
                     /locus_tag="ON345_004064"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3074010.1"
                     /translation="ISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDA
                     AAEQPESLAEDPKTLSISCEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT           87 a           94 c           81 g           74 t
ORIGIN      
        1 ccatctcaaa acttgctctt gaaaatggca ttaacgccaa tctgttgttc aaatggcgac
       61 aacaatggcg cgagggaaag ctgctattac cttcttcaga gagcccccag ctacttcctg
      121 tgactctcga tgcagctgcc gaacagccag aatcgctcgc agaggacccg aaaaccctca
      181 gtatcagctg tgaggtaacg ttccggcacg ggacgctccg cttcaatggc aatgtcagcg
      241 aaaagctcct gactctgctg atacaggaac tgaagcgatg atcccgttac cttccgggac
      301 caaaatttgg ctggttgccg gtatcaccga tatgag
//
LOCUS       DAJSKJ010000442          333 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000442 DAJSKJ010000000
VERSION     DAJSKJ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.452"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            35..307
                     /locus_tag="ON345_004065"
                     /pseudo
     CDS             35..307
                     /locus_tag="ON345_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           73 a           94 c           83 g           83 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 cgcccgcatt atgggcgttg gcctcaacac gattttccgc catttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agt
//
LOCUS       DAJSKJ010000443          331 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000443 DAJSKJ010000000
VERSION     DAJSKJ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.454"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT           94 a           84 c           68 g           85 t
ORIGIN      
        1 taattaatac cgtagatatt cattcatctt accctgtgca ccaaaagcaa aaactcactg
       61 acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga tgcgctacgc ttatcaggcc
      121 tacgccatct ctgcaatata ttgaatttgc gtgcttttgt aggcaggata aggcgttcac
      181 gccgcatccg gcatgaataa agcgcactca acaatctgaa acccgccgga acggtttatt
      241 acgtaaatca ggtgaaactg accgataagc cgctttcttt tgggtatagc gtcgtggaca
      301 gtcattcatc tttctgcccc tccaaaagca a
//
LOCUS       DAJSKJ010000444          324 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000444 DAJSKJ010000000
VERSION     DAJSKJ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.455"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>324
                     /locus_tag="ON345_004066"
     CDS             <1..>324
                     /locus_tag="ON345_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3074011.1"
                     /translation="GWMRYRNSRQVTGVVKNVTVSQSSGKWYISIQTESEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFEPVNSLQKNQKKLARLQRQLSRKVKFSNNWQKQKCK
                     IQRLHS"
BASE COUNT           94 a           78 c           89 g           63 t
ORIGIN      
        1 ggctggatgc gctaccggaa tagccgtcag gtcacgggag ttgtgaaaaa tgtcacggtc
       61 agccagtcca gcggtaagtg gtacatcagt attcagacag aaagtgaagt atccactccg
      121 gttcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact cgccacgctg
      181 tcagatggca cagtctttga gcctgtaaac agtttgcaga aaaaccagaa gaagctggcg
      241 agacttcagc ggcagttaag ccgcaaggtc aaattcagca acaactggca gaagcagaaa
      301 tgcaaaatac agcgactgca ttcc
//
LOCUS       DAJSKJ010000445          321 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000445 DAJSKJ010000000
VERSION     DAJSKJ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.456"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..204)
                     /locus_tag="ON345_004067"
     CDS             complement(<1..204)
                     /locus_tag="ON345_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR3074012.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGI"
BASE COUNT          107 a           70 c           70 g           74 t
ORIGIN      
        1 gatccctggc aatgccgccc agctgaaaaa catcatcatg ctgatcatca gtgccagagg
       61 cagataaaag agaaaaacct caccggaaga aatatgcgcg aaagtattaa tgtgggcgaa
      121 aatgggcaag attaaagaag gcaggaacag cggaagcccc caccatttat cacgtatttt
      181 tatataagtt gcattcagtt tcatagatgc tcagcagaat cccccacatc ctgaaggagg
      241 tgtattcaga caggcatccc acctgacttc gaatgatgat tattcatcac tatagagagc
      301 attgattcta agtgtcatat g
//
LOCUS       DAJSKJ010000446          281 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000446 DAJSKJ010000000
VERSION     DAJSKJ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.457"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>281
                     /locus_tag="ON345_004068"
     CDS             <1..>281
                     /locus_tag="ON345_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR3074013.1"
                     /translation="VYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADW
                     AINNSQTPLGEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLM"
BASE COUNT           76 a           59 c           76 g           70 t
ORIGIN      
        1 tggtttatgg tgccggtcca atcggcctga cgatcgttca ggtattaaaa ggcgtctata
       61 acgttaaaaa tgtgattgtt gccgatcgca ttgatgaacg actggaaaaa gcgaaagaga
      121 gcggggcaga ctgggcgatt aataacagcc agacaccgct tggcgagatt ttcgctgaaa
      181 aaggcatcaa gccgacatta attatcgatg cggcttgtca tccttctatc ctgaaagagg
      241 ccgtaacgct ggcttctcca gcggcacgta ttgtattgat g
//
LOCUS       DAJSKJ010000447          278 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000447 DAJSKJ010000000
VERSION     DAJSKJ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.458"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            24..100
                     /locus_tag="ON345_004069"
     tRNA            24..100
                     /locus_tag="ON345_004069"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ON345_004070"
     tRNA            143..218
                     /locus_tag="ON345_004070"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAJSKJ010000448          276 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000448 DAJSKJ010000000
VERSION     DAJSKJ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.459"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>276
                     /gene="pgrR"
                     /locus_tag="ON345_004071"
     CDS             <1..>276
                     /gene="pgrR"
                     /locus_tag="ON345_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR3074014.1"
                     /translation="SGTANRWRLIRGGREGRVRMEGQLLLNTIDLIIDAAIDGHGLAY
                     LPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFI"
BASE COUNT           77 a           54 c           66 g           79 t
ORIGIN      
        1 atcgggtaca gcaaatcgct ggagattaat acgcggtgga cgtgaaggtc gtgttcgcat
       61 ggaaggtcag cttttactga atacgataga cctgatcatt gatgctgcaa ttgatgggca
      121 tggattggcg tatctacctt atgatcaggt tgagcgggct attaaagaaa aaaaactgat
      181 acgtgttttg gataaattca caccagattt acccggttat cacctgtact atccacaccg
      241 tcgacatgct ggctcagcat tctcattatt tataga
//
LOCUS       DAJSKJ010000449          272 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000449 DAJSKJ010000000
VERSION     DAJSKJ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.460"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..263
                     /locus_tag="ON345_004072"
     CDS             <1..263
                     /locus_tag="ON345_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3074015.1"
                     /translation="ADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEH
                     FISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT           74 a           56 c           76 g           66 t
ORIGIN      
        1 cagcagatta tcaggcgcaa ctgaagcggc ataatctgcg tggaagtatg agcgcaaaag
       61 gttgctgcta cgataatgcc tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgta
      121 tccatggaga acactttatc agccgggaaa taatgcgggc aacggtattt aattatatcg
      181 aatgtgatta caatcggtgg cggcggcaca gttggtgtgg cggcctcagt ccggaacaat
      241 ttgaaaacca gaacctcgct taggcctgtg tc
//
LOCUS       DAJSKJ010000450          272 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000450 DAJSKJ010000000
VERSION     DAJSKJ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.461"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(19..272)
                     /locus_tag="ON345_004073"
                     /pseudo
     CDS             complement(19..272)
                     /locus_tag="ON345_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 family protein"
BASE COUNT          108 a           62 c           41 g           61 t
ORIGIN      
        1 cgactctcat agattttact aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc
       61 acaaaccaga caaataccga gcgagaatac atatactcaa aactcgaaca tttcccgcat
      121 aaagagtttt cttaagataa gaataataag tggcgtaaga agaaaaaatg ctgcaatcat
      181 tgcttgcaat atacgatctt cgcctgaaac ggaaggtata ctgcaaatat tgcctcccga
      241 gtcaagcatc cagtcatact tattgaaagc aa
//
LOCUS       DAJSKJ010000451          271 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000451 DAJSKJ010000000
VERSION     DAJSKJ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.462"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            4..213
                     /gene="fumD"
                     /locus_tag="ON345_004074"
     CDS             4..213
                     /gene="fumD"
                     /locus_tag="ON345_004074"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR3074016.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT           83 a           51 c           77 g           60 t
ORIGIN      
        1 agaatgggaa atcggacaaa agaggatgaa ctgtaccgag aaatgtgcag agttgtcggt
       61 aaagttgtgc tggaaatgcg cgatctgggg caggaaccaa agcatattgt tatcgcaggc
      121 gtattacgta cagcattagc caataaacgc attcagcgca gtgagctgga aaagcaggcg
      181 atggaaaccg tcattaacgc actggtaaaa tgaccattca ggatgaggcg gtaagcttca
      241 tcctgatatt cttgcttcag ttgcggtcca g
//
LOCUS       DAJSKJ010000452          271 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000452 DAJSKJ010000000
VERSION     DAJSKJ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.463"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT          105 a           41 c           44 g           81 t
ORIGIN      
        1 attaagcaac accagttatt gcatattttc tacatactca tcaatgttca aagcagcaga
       61 tctattactt attgatggaa taagttggtt gcttgaattt cagttagaaa aaaaaccaat
      121 catattttta gaacgtgagg atcatcttcc tttcgatata aacggtgaaa agatccgtga
      181 tggattaaac tcagtaaata acattgatca agcactcgag ttattgcttg aattcaggaa
      241 tggaaaagta gacacaaaaa taaaaaatca a
//
LOCUS       DAJSKJ010000453          266 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000453 DAJSKJ010000000
VERSION     DAJSKJ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.464"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>266
                     /locus_tag="ON345_004075"
     CDS             <1..>266
                     /locus_tag="ON345_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3074017.1"
                     /translation="ILREAPEMKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRT
                     RISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNV"
BASE COUNT           60 a           74 c           75 g           57 t
ORIGIN      
        1 atacttcgcg aagcgcctga aatgaagtat gtctttattg aaaaacatca ggctgagttc
       61 agcatcaaag caatgtgccg cgtgctccgg gtggcccgca gcggctggta tacgtggtgt
      121 cagcggcgga caaggataag cacgcgtcag cagttccgcc aacactgcga cagcgttgtc
      181 ctcgcggctt ttacccggtc aaaacagcgt tacggtgccc cacgcctgac ggatgaactg
      241 cgtgctcagg gttacccctt taacgt
//
LOCUS       DAJSKJ010000454          266 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000454 DAJSKJ010000000
VERSION     DAJSKJ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.465"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..215)
                     /locus_tag="ON345_004076"
     CDS             complement(<1..215)
                     /locus_tag="ON345_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR3074018.1"
                     /translation="MSWSHYLVGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKK
                     IEVALGHYNAEQFRSVGDRSPKIPFTIP"
BASE COUNT           81 a           57 c           53 g           75 t
ORIGIN      
        1 ggaatagtga atggaatttt tgggctacga tcgccaacag aacgaaactg ttcggcatta
       61 tagtgaccaa gagcaacttc aattttctta tcagagtcat tgatttcaca agcggatttt
      121 aaaatctcgc catcaaaaac tatttcacca tcggttgcag cggcattacc tgcgaaaagc
      181 gcaagtaagc ccacaaggta atgactccaa cttattgata gtgttttatg ttcagataat
      241 gcccgatgac tttgtcatgc agctcc
//
LOCUS       DAJSKJ010000455          260 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000455 DAJSKJ010000000
VERSION     DAJSKJ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.466"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>260
                     /locus_tag="ON345_004077"
     CDS             <1..>260
                     /locus_tag="ON345_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3074019.1"
                     /translation="RKTAISARRVALQIIHSNIISAPDELREQLRNMTRMQLIRTLGS
                     WRPDASEYRNVTNVYRISLKSLARRYLELHDEIADLDVMIAA"
BASE COUNT           72 a           70 c           55 g           63 t
ORIGIN      
        1 gccgaaaaac agcaatatca gcccgcagag tcgctctcca gattatccat tcaaatatta
       61 tctctgcccc ggatgaatta cgtgaacagc tcagaaatat gacgcgcatg cagctcatca
      121 ggaccctggg ttcctggaga cctgatgcca gtgaataccg caatgtaacc aacgtttatc
      181 gcatttcatt aaagtccctt gcccgacgct atctcgagtt acatgacgaa atcgctgatc
      241 tggatgtcat gattgcggca
//
LOCUS       DAJSKJ010000456          258 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000456 DAJSKJ010000000
VERSION     DAJSKJ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.467"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..200)
                     /locus_tag="ON345_004078"
     CDS             complement(<1..200)
                     /locus_tag="ON345_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3074020.1"
                     /translation="MTKNTRFSPAVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWIRQHERDTGCSDGGLTT"
BASE COUNT           59 a           70 c           63 g           66 t
ORIGIN      
        1 gtggtgagcc caccatcact gcacccggta tcccgctcat gctggcgaat ccagacacgc
       61 agagtctccg gtgtacagcc aatctttggg gcaatggaac aaattgccgc ccattgtgag
      121 tcatattcgc cctgactttc cagaaccata cgaactgccc gttgacggac tgcaggggaa
      181 aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt ctccaggatt
      241 tctggggcgg ttcaaaat
//
LOCUS       DAJSKJ010000457          245 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000457 DAJSKJ010000000
VERSION     DAJSKJ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.468"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT           85 a           36 c           41 g           83 t
ORIGIN      
        1 gagaaaagag atgcaatcat tgacgggagt agagataaat actccttgcg tagaaagata
       61 atccgcatat taatctgacc atcattttat ataaaacggc atatttaaac gcattataca
      121 atatgggtta tctttctttt gtaaattgtc caacccgatg caatggctat tttgttatta
      181 tttgatgaat aatatcagtg cgtcataatt caagttaata accttaaggg ataccagtta
      241 tattt
//
LOCUS       DAJSKJ010000458          242 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000458 DAJSKJ010000000
VERSION     DAJSKJ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.469"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT           74 a           50 c           45 g           73 t
ORIGIN      
        1 tcctacaatt gtaagactaa aatacttctt gcgataataa ctacaactgt aagataaccc
       61 tttcaaaatg accgttgctc tctgatttct catttcatgc tcacccaata tgatggcggc
      121 gttttctaaa actgttaaag aatgaggtaa gtatgaaacg tttaattatg gctacgatgg
      181 tcacagcaat tctggcatct tcaactgtat gggctgctga taatgcaccg gtagcggcac
      241 aa
//
LOCUS       DAJSKJ010000459          224 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000459 DAJSKJ010000000
VERSION     DAJSKJ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.470"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>224
                     /locus_tag="ON345_004079"
     CDS             <1..>224
                     /locus_tag="ON345_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR3074021.1"
                     /translation="MSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNL
                     NLRQHLARLGRKSLSFSKSVELHDKVIGHYL"
BASE COUNT           55 a           53 c           68 g           48 t
ORIGIN      
        1 atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat
       61 gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag
      121 cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc
      181 tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atct
//
LOCUS       DAJSKJ010000460          223 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000460 DAJSKJ010000000
VERSION     DAJSKJ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.471"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>223
                     /locus_tag="ON345_004080"
     CDS             <1..>223
                     /locus_tag="ON345_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR3074022.1"
                     /translation="MRKMESNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGD
                     TYNLIKTLDICHYDSERKVLTSHHGIIRDE"
BASE COUNT           86 a           34 c           41 g           62 t
ORIGIN      
        1 tatgcgaaaa atggagagta ataaagatag ccgtattaat aaaaatgtcg tcgttctggc
       61 tggaaatcat gaaatcaatt ttaatggtaa ctatactgca cgactagcaa atcataaact
      121 ctctgccggt gatacgtata acttaattaa aacgctcgat atttgtcatt atgactcaga
      181 aaggaaagtt ttaaccagtc atcacggaat aataagagat gaa
//
LOCUS       DAJSKJ010000461          215 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000461 DAJSKJ010000000
VERSION     DAJSKJ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.472"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
BASE COUNT           57 a           53 c           63 g           42 t
ORIGIN      
        1 agacaagccg tcaggccctt cttgagtcac gcgcatcggt gaggagatgc ctcgcatgaa
       61 ggcgcttccg gcaaccgacg ggtccagggc aggacacaaa aggtcgatcg ctgtgtgagt
      121 caggatgcgg gaaggtcttc actgcatcag taatcacacc aatctggtta aacggcaaac
      181 taagtggtgg taaagactga catcggcatt attac
//
LOCUS       DAJSKJ010000462          213 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000462 DAJSKJ010000000
VERSION     DAJSKJ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.473"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..>213)
                     /locus_tag="ON345_004081"
     CDS             complement(<1..>213)
                     /locus_tag="ON345_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR3074023.1"
                     /translation="KASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLR
                     TDEGWLYLAVVIDLWSRAVIGWSMSPR"
BASE COUNT           47 a           64 c           57 g           45 t
ORIGIN      
        1 gcgtggcgac attgaccagc caataacggc acgtgaccac aggtcaatga ccactgccag
       61 atacagccag ccttcatctg tacgtaagta cgtgatgtct cctgcccact tctggttcgg
      121 gccactggcg taaaaatcct gctccaacag attttctgac acaggcaggc cgtgtgcgcg
      181 gtagctgacc gggctgaact tccgggaggc ctt
//
LOCUS       DAJSKJ010000463          212 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.474, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000463 DAJSKJ010000000
VERSION     DAJSKJ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.474"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..>212
                     /locus_tag="ON345_004082"
     CDS             <1..>212
                     /locus_tag="ON345_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylglyoxal detoxification protein"
                     /protein_id="HCR3074024.1"
                     /translation="EGGNTVVIGYVPRISIPSNGKKGDKIWQSSEYFMNVFCNNALPA
                     PSPGEEYPSAWTNIMMFLPGGQDFYN"
BASE COUNT           67 a           39 c           45 g           61 t
ORIGIN      
        1 tgaaggggga aataccgtag tcattggtta cgtaccaaga atttctatcc ccagtaatgg
       61 taaaaaaggt gataagatct ggcaaagcag tgagtatttt atgaatgtct tctgtaataa
      121 tgcattacct gccccatcac ctggagaaga atacccatca gcctggacta atataatgat
      181 gtttttacca ggcggtcagg acttttataa tc
//
LOCUS       DAJSKJ010000464          202 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000464 DAJSKJ010000000
VERSION     DAJSKJ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.denovo.475"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            <1..191
                     /locus_tag="ON345_004083"
     CDS             <1..191
                     /locus_tag="ON345_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3074025.1"
                     /translation="AVMENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKL
                     SLGGLSPVEYRTEYQKTG"
BASE COUNT           74 a           36 c           48 g           44 t
ORIGIN      
        1 atgcggtaat ggagaatttc tttggtcacc tgaaagaaga aatgtactat cgccgggact
       61 acagaagtgt agaagagctg gaaaatgccg ttaacgaata cataacttac tggaatcaaa
      121 aaagaataaa actcagtctt gggggactca gcccggtaga ataccggact gagtatcaaa
      181 aaaccggtta actgaaactg tc
//
LOCUS       DAJSKJ010000465        11413 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.guided.124, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000465 DAJSKJ010000000
VERSION     DAJSKJ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.guided.124"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            complement(<1..148)
                     /locus_tag="ON345_004084"
     CDS             complement(<1..148)
                     /locus_tag="ON345_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HCR3074026.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEE"
     gene            complement(145..459)
                     /locus_tag="ON345_004085"
     CDS             complement(145..459)
                     /locus_tag="ON345_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3074027.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(511..2013)
                     /gene="yjeM"
                     /locus_tag="ON345_004086"
     CDS             complement(511..2013)
                     /gene="yjeM"
                     /locus_tag="ON345_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR3074028.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(2232..3209)
                     /gene="epmA"
                     /locus_tag="ON345_004087"
     CDS             complement(2232..3209)
                     /gene="epmA"
                     /locus_tag="ON345_004087"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR3074029.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            3534..5342
                     /gene="frdA"
                     /locus_tag="ON345_004088"
     CDS             3534..5342
                     /gene="frdA"
                     /locus_tag="ON345_004088"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR3074030.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            5335..6069
                     /gene="frdB"
                     /locus_tag="ON345_004089"
     CDS             5335..6069
                     /gene="frdB"
                     /locus_tag="ON345_004089"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR3074031.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            6080..6475
                     /gene="frdC"
                     /locus_tag="ON345_004090"
     CDS             6080..6475
                     /gene="frdC"
                     /locus_tag="ON345_004090"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR3074032.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            6486..6845
                     /gene="frdD"
                     /locus_tag="ON345_004091"
     CDS             6486..6845
                     /gene="frdD"
                     /locus_tag="ON345_004091"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR3074033.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            6908..8041
                     /locus_tag="ON345_004092"
     CDS             6908..8041
                     /locus_tag="ON345_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR3074034.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGSDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            8130..8663
                     /gene="blc"
                     /locus_tag="ON345_004093"
     CDS             8130..8663
                     /gene="blc"
                     /locus_tag="ON345_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR3074035.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(8660..8977)
                     /gene="sugE"
                     /locus_tag="ON345_004094"
     CDS             complement(8660..8977)
                     /gene="sugE"
                     /locus_tag="ON345_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR3074036.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(9153..9299)
                     /gene="ecnB"
                     /locus_tag="ON345_004095"
     CDS             complement(9153..9299)
                     /gene="ecnB"
                     /locus_tag="ON345_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR3074037.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(9410..9535)
                     /gene="ecnA"
                     /locus_tag="ON345_004096"
     CDS             complement(9410..9535)
                     /gene="ecnA"
                     /locus_tag="ON345_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR3074038.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(9587..10153)
                     /gene="efp"
                     /locus_tag="ON345_004097"
     CDS             complement(9587..10153)
                     /gene="efp"
                     /locus_tag="ON345_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3074039.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            10195..11223
                     /gene="epmB"
                     /locus_tag="ON345_004098"
     CDS             10195..11223
                     /gene="epmB"
                     /locus_tag="ON345_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR3074040.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLCQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2966 a         2967 c         2847 g         2633 t
ORIGIN      
        1 cttcttcgag atacatcaac acatcgcaga cgttgttaat cccgcgtcca tcaatcatcc
       61 actctttgtc cagcgcggaa gtgatggtta tccagaggaa cgccacaata aaccagatgc
      121 agcataagac aaacatgcgc gcactcataa ggtaaccggt cgcttaattt cgctatagcg
      181 catacgaaac aaatcgccgt atcccactaa ttccccgcga aaaaccagac atcggataag
      241 ataagcgcaa taaatatccg gcaggtttcc ccaccgttta ccatcgcgat ctttgagggt
      301 gttaatggtg ctacccacca ccatcgccac tttacgggtg tggcaggcat cgacaaaaga
      361 gagaatcgtc tgctctattt ccgctacatc cccggcagaa aattgcagcg ccctgatttc
      421 atcttcggtt attgccgggt cccggctggt gtcatccata acatttcctg tctttacgcc
      481 ctatcgtcag gtagacgata gggcatcggg ttattccacc gcccactgcg gattttttgc
      541 cactctgctg caatagttct ggtaaatcgc catcgctaac agcgagaaga agacagggcc
      601 gccaatcatc cacaatgtgc tgtcccagtc tccggcttca accacaggtt gaatgatggt
      661 gaagacgttc gcaaatgtca ccaccagtac gacaaccact gtcgcaatca ttgccgacaa
      721 atgcgttttg aaaatcacaa acggtctgtc gagatcctga cgtgctttaa agaacgggaa
      781 agccagcgcg aggaacaggt aaggaagcgt catagacacg ttcgccatca gcgtcagctt
      841 gttaaagaac gccgatgcgg taccgccacc aaacgaaacc agcaggatga agacagtaac
      901 caacccgcac tgcatccaca tggcgataga aggcatcccc atcgcattca ggcgcgtcat
      961 cggttccggc cacaacgctt tcggcgtccc ctggatgatg gctttcagcg gtgaatagca
     1021 aagcgtaaag aacgccccgg tataagcgag gaacatcgaa agtccggtaa tacgcgcaaa
     1081 ccatacaccc agcgacaatg acgcttcagg tgacaaatgc agtgcgttac ccagcgtcac
     1141 cccgaggctc ttcatcagca cataggtaat attgccgagg ttaacggaac cattacttaa
     1201 tacctgctgc cagtttgtgc tgacacccca taaaaatatt gccagcgaat aaccgattga
     1261 aataacaata gcggcaaaaa caataccttt ggcaaagttc ttttctggat tttccgtttt
     1321 atcgaccaga ccaccaaccg cttcaattcc gccataggca aaaatagcaa ataccacaaa
     1381 cgatagcatt gccagaccgg actgataacc tgggttcggt gatgcaagga aattaatatc
     1441 ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg cttactaaca gcaatactaa
     1501 attcagacac attactgcaa taccgcccac cgcagtaatg cgggcaattt tattaatccc
     1561 ctttgaagca acgacggtga ccagaatcat ccatgccact gccagcagac caaccacctg
     1621 cgtaggctcc agtccggcaa tacgccagtg ctgggtcatg tcgctaccat agaggaatgt
     1681 tgagaacggt acccaaactt tcgcggaggt actcaccatc cagatgatat aagaggaaaa
     1741 ccacataaac gtaccaatga aggcaaaacg tggtccgaca ctattattca tccaggaata
     1801 gataccacct tcttctttgc gataagcagc tcccatttca gccatcatta aggcgaatgg
     1861 aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac ccattaagta
     1921 ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca atatgagtcc
     1981 tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt cagcgcgtgt
     2041 ataacacgtg cccactgaac tgtactgcgc aacattgagc aggcagcgta aaaaccaata
     2101 cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct ttaacggaaa
     2161 aatgaaaaca tatgctgcag aatggcgctt atcacgccat tcttcgctgt taattcagta
     2221 atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag tgtctccgcg
     2281 cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca gtcaggcata
     2341 ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa accgcgcgcc
     2401 gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc cgttaattca
     2461 tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc agcgacccga
     2521 tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg gtacacaaag
     2581 gtcggttttt ctttaccaat atttggctct acgccaaagg taaacagcaa ttgtagcaac
     2641 gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc tgcgacttcc
     2701 cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa gaaagcttgt
     2761 tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag atcgtccacc
     2821 tcgttcatca accggtacat atcatagtgc ggtcgatacc actccagcat agtgaactca
     2881 gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg gaataccggc
     2941 ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat taaccagaga
     3001 ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac caaatgaata
     3061 tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc acgatcggca
     3121 aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg aatggatgcg
     3181 ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg
     3241 aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc
     3301 agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata
     3361 atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag
     3421 actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag
     3481 gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa
     3541 cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg
     3601 ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc
     3661 gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct
     3721 tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg
     3781 tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg tgggggtgcc
     3841 catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg
     3901 agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga
     3961 cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg
     4021 ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc acgctggtgc
     4081 agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt tatcgttaca
     4141 acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc
     4201 cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc
     4261 tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt
     4321 atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata
     4381 tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca
     4441 acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac ctcggcgaga
     4501 aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg
     4561 atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg ggcggtatcg
     4621 aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct
     4681 ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa ctggtggtct
     4741 tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt aatggcaacg
     4801 aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctggttaacc
     4861 aggatggtgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca atggaagaag
     4921 gctgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggctgagc
     4981 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacttc cagcgtgttc aacaccgacc
     5041 tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg gcgcactccg
     5101 caatggcacg taaagagtcc cgcggcgcgc accagcgtct ggacgaaggt tgcaccgagc
     5161 gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
     5221 gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
     5281 gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag gcgaatggct
     5341 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
     5401 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc
     5461 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
     5521 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
     5581 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
     5641 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
     5701 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
     5761 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
     5821 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
     5881 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggcgta
     5941 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     6001 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     6061 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     6121 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     6181 tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc
     6241 ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     6301 gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc
     6361 ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     6421 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     6481 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     6541 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     6601 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     6661 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     6721 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat
     6781 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt atcgttacaa
     6841 tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     6901 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt gctccacatt
     6961 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     7021 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     7081 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     7141 aggttcggtc agcaaaacat ttacgggcgt gcttggtagc gacgctattg ctcgagggga
     7201 aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta aacagtggaa
     7261 tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc
     7321 ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     7381 ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     7441 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact
     7501 caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg
     7561 atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg
     7621 tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga atccccgtga
     7681 tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct actggcaaac
     7741 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa atcctgacag
     7801 catcattaac ggcagtggca ataaaattgc actggcagca caccccgtaa aagcgattac
     7861 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa ccggcggatt
     7921 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc tggctaacaa
     7981 aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg ctctacagta
     8041 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     8101 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     8161 cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc gtgacagtag
     8221 tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc cgttttgatc
     8281 accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt gatgacggcg
     8341 gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag cagagtgaag
     8401 ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca ttctttggtc
     8461 ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat gcgctggttt
     8521 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     8581 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     8641 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     8701 ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc acaatgccgg
     8761 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     8821 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acagtaataa
     8881 cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc cataccactt
     8941 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     9001 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     9061 ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca gaaaaaacaa
     9121 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     9181 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     9241 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     9301 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     9361 atcgccaaaa atgacggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     9421 cgcgagagat ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac
     9481 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     9541 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     9601 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     9661 gaaccttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     9721 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     9781 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     9841 tagcgttatc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     9901 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     9961 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
    10021 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
    10081 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
    10141 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
    10201 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
    10261 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
    10321 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
    10381 atggagaaag gcaatccgga cgatcctctt ttgtgtcagg tacttacctc gcaagatgag
    10441 tttgtcgtcg cgtccggatt ctccaccgac ccgctggaag aacagcacag cgtagtgcct
    10501 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
    10561 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
    10621 aactggcaga ctgctcttga gtatgttgct gcgcatccgg aactggacga gatgattttc
    10681 tccggcggcg atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg
    10741 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
    10801 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
    10861 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
    10921 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
    10981 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
    11041 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
    11101 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gttatctggt gccgaaactg
    11161 gcgcgagaaa tcggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
    11221 taactgatta aacagggaag ttaaacatta aaattgaatg gattttcaat gaactgtagg
    11281 ccggataagg cgtttacgcc gcatccggca agagcaacaa acgcaagtac agcaacctgt
    11341 cattaaactg ggaaatttaa tctttgcacg ttgcataacc tttaattatt catatgcatt
    11401 gctgttgtta ctc
//
LOCUS       DAJSKJ010000466         9140 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.guided.158, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000466 DAJSKJ010000000
VERSION     DAJSKJ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.guided.158"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            169..1167
                     /gene="flgK"
                     /locus_tag="ON345_004099"
                     /pseudo
     CDS             169..1167
                     /gene="flgK"
                     /locus_tag="ON345_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            1281..2204
                     /gene="lafU"
                     /locus_tag="ON345_004100"
     CDS             1281..2204
                     /gene="lafU"
                     /locus_tag="ON345_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR3074041.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            2275..3330
                     /gene="dinB"
                     /locus_tag="ON345_004101"
     CDS             2275..3330
                     /gene="dinB"
                     /locus_tag="ON345_004101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3074042.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3327..3779
                     /locus_tag="ON345_004102"
     CDS             3327..3779
                     /locus_tag="ON345_004102"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3074043.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            4025..5164
                     /locus_tag="ON345_004103"
     CDS             4025..5164
                     /locus_tag="ON345_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR3074044.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQHVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            5161..5775
                     /locus_tag="ON345_004104"
     CDS             5161..5775
                     /locus_tag="ON345_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR3074045.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5832..7289)
                     /gene="pepD"
                     /locus_tag="ON345_004105"
     CDS             complement(5832..7289)
                     /gene="pepD"
                     /locus_tag="ON345_004105"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR3074046.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7550..8008
                     /gene="gpt"
                     /locus_tag="ON345_004106"
     CDS             7550..8008
                     /gene="gpt"
                     /locus_tag="ON345_004106"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3074047.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8100..>9140
                     /gene="frsA"
                     /locus_tag="ON345_004107"
     CDS             8100..>9140
                     /gene="frsA"
                     /locus_tag="ON345_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR3074048.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMLSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQ"
BASE COUNT         2219 a         2316 c         2469 g         2136 t
ORIGIN      
        1 atgcctgatg cgacgctgtc gcgtcttatc aggcctacga gtcgcccgta tgtaggccgg
       61 ataaggcgtc cacgccgcat ccggcgttcg gcacccggtt tacggaactt aagaatcccc
      121 aactttacgc cgcttttaac cggcacgatt taccggtaag gatccaacat ggacatgatt
      181 aacatcggct acagcggtgc atcaaccgcg caggtggagc ttaacgtcac ggcgcaaaac
      241 accgccaacg ccatgaccac gggctacacc cgtcaggtgg cggagatcag caccatcggt
      301 gccagcggcg gttcgccgaa cagcgccggt aacggcgtac aggtcgacag cattcgccgc
      361 gtctccaacc agtatcaggt gaatcaggtg tggtatgccg ccagcgatta cggctattac
      421 agcacccagc aggggtatct cagccaactg gaagcggtac tgagcgacga taacagcagc
      481 ctgagcggcg gcttcgataa cttcttcgcc gcccttaacg aagcaaccac cagccccgat
      541 gattccgccc tgcgcgagca ggtgatcagt gaagccgggg cgctgtcgtt gcgtattgat
      601 aacacgctgg attacatcga ctcgcaaagc acggaaatca tcagccagca gcaggcgatg
      661 gtgtcgcaaa tcaatacgct caccagcggc atcgccagct ataaccagca aatcgctcag
      721 gctgaagcca atggcgataa cgcctccgcg ctgtacgacg cccgcgacca gatggtggaa
      781 gaactgagcg ggatgatgga tgtgcaggtc aatatcgacg accagggcaa ctacaacgtt
      841 accctgaaaa acggtcagcc gctggtgagt gggcagcaaa gctcgaccat cgcgctggaa
      901 accaacgccg atggcacgcc aaccatgtcg ctgacgttcg ctggcaccac ctcgacgatg
      961 accaccgaca ccggcggttc attaggcgca ctgtttgatt atcaaaacga tgtgctgacg
     1021 ccgctgaccg acaccatcaa cagcatggcg tcgcagttcg ccgatgcggt caacaaccag
     1081 ctggcgcagg gctacgatct caacggtaac cccggcgagc cgctgtttat ttatgacgcc
     1141 agcaatgccg atggcccgct gaccgttaaa ccgacgctgc tggcggtgga tgccgggcgt
     1201 aagctgctgc atctggcatc taaaccgacc ttcgccaacc tcgacgcgtg ggtgaatgcg
     1261 atgctggagc aagaataaac atgagaaaga cgggcaaccg ccgcgagcgt ggggcaaaaa
     1321 ccaccatcgt ccggcggcag atcaaaaaga atcatgccgg gcaccacggc ggggcgtgga
     1381 aggtagcgtt cgccgacttt actttggcaa tgatggcgct gtttatgacg ctgtggattg
     1441 tcaacagcgt cagtaaatcc gagcgtgaaa gtattatcgc cgctctgcac gggcagtcga
     1501 tttttaacgg tggtgggctg tcgccgttaa ataaaatcag cccgtctcat ccgccaaaac
     1561 ctgccaccgt ggcggtgccg gaagagacgg agaaaaaagc gcgtgatgtg aatgagaaaa
     1621 cggcgctgct gaagaagaaa tccgccaccg agcttggcga gctggcaacc agtatcaaca
     1681 ccattgcccg tgatgcgcat atggaagcga atctggagat ggagattgtt ccccagggat
     1741 tacgcgtgct gattaaagac gaccagaacc gcaatatgtt cgaacgcggc agcgcgcaga
     1801 ttatgccgtt ctttaaaact ttgctggtgg agctcgcgcc agtgttcgac tcgctcgata
     1861 acaaaattat tattaccggg cataccgatg cgatggccta caaaaacaat atctacaaca
     1921 actggaacct ttcgggcgat cgcgcgcttt cggctcgtcg ggtgctggaa gaggccggaa
     1981 tgccggaaga taaagtgatg caggtaagcg caatggcgga ccagatgctg ctggacgcga
     2041 aaaatccgca aagcgcgggc aaccggcgca ttgagattat ggtgctgacc aaaagtgcgt
     2101 ccgatacgct gtatcaatac tttggtcagc atggggataa agtggtgcag ccgctggtgc
     2161 aaaagctgga taagcagcag gtgctttcgc agcgaacgcg ttaaatgctg aatctttacg
     2221 catttctcaa accctgaaat cactgtatac tttaccagtg ttgagaggtg agtaatgcgt
     2281 aaaatcattc atgtggatat ggactgcttt ttcgcagcgg tggagatgcg cgacaatccc
     2341 gccctgcgcg atatccctat tgctattggc ggcagccgtg aacgtcgggg ggtgatcagc
     2401 accgccaatt atcccgcgcg taaatttggc gtacgtagcg ctatgccgac agggatggcg
     2461 ctcaaattat gcccgcatct caccttgctt ccggggcgct ttgacgccta caaagaagcc
     2521 tcaaatcata tccgtgaaat cttctcgcgc tacacctcgc gtattgaacc gttgtcactg
     2581 gatgaggctt atctcgacgt caccgatagc gtccattgcc acggttctgc gaccctcatc
     2641 gcccaggaaa tccgccagac gattttcagc gagctgcaac tgacagcgtc tgcgggcgtg
     2701 gcaccagtaa agtttctcgc caaaatcgcc tccgacatga ataaacccaa cggtcagttt
     2761 gtgattacgc cggcagaagt tccggcattt ttacaaacct taccgctggc aaaaatcccc
     2821 ggcgtcggca aagtctcggc ggcaaaactg gaagcgatgg ggctgcggac ctgcggtgat
     2881 gtacaaaagt gtgatctggt gattctgctt aaacgctttg gcaaatttgg ccgcattttg
     2941 tgggagcgta gtcaggggat tgacgagcgc gacgttaaca gcgaacggtt acgaaaatcc
     3001 gtcggcgtgg aacgcacgat ggcggaagat attcatcact ggtctgaatg tgaagcgatt
     3061 atcgagcggc tgtatccgga acttgaacgc cgtctggcaa aggtaaaacc tgatttactg
     3121 atcgcccgtc agggagtgaa attaaagttt gatgattttc agcaaaccac tcaggagcac
     3181 gtctggccgc ggctgaataa agctgactta atcgccaccg cgcgtaaaac ctgggatgaa
     3241 cgccgcggcg ggcgcggtgt gcgactggtg gggctgcatg tgacgttgct tgacccgcaa
     3301 atggaaagac aactggtgct gggattatga ataacataca aataagagac tatcagcctg
     3361 gcgattttca gcaactatgc gctattttca ttagagcggt tacgatgact gccagtcagc
     3421 attattcacc acaacaaatt gccgcctggg cgcagattga cgaatctcgc tggaaggaga
     3481 aactcgcgaa atcacaagtg cgggttgcag tcattaatgc aaaactggtc ggctttatta
     3541 cctgcgttga acactatatc gatatgttat ttgttgaccc tgaatacacc cgccgtgggg
     3601 ttgccagcgc tttgttaaaa cctttgatta agtctgaatc cgaacttacg gtggacgcaa
     3661 gcataaccgc aaaacccttt tttgaacgtt atggttttca gacagttaag cagcaacgcg
     3721 ttgaatgtcg gggagagtgg tttattaatt tttatatgcg ttataaacag caacattaaa
     3781 tccagcttgc aatgaaaata acgcaagcct agtatgtgcg cgttttctta ttccacaaac
     3841 tgcaaggagg taaatcatga caaatccttt atcaatgact ctttgcagac ctttccagga
     3901 ttaattcttt ttttcttgcc ctggattcgt ctgccatttc ctgatttttt aattgttact
     3961 ctcaataatt caagtcgcaa cacatgcaac ttgaagtatg acgagtctat ttacggaatg
     4021 gtatatgggc aattatattc gtcccttatc cgatgcggta tttaccatcg catctgatga
     4081 cctgtggatc gagagtttag cgatccaaca attacacacc acggcaaatt tacccaacat
     4141 gcagcgcgta gtggggatgc cagatttaca tcccggacgc ggctatccga ttggcgcagc
     4201 gttcttctct gttggtcgtt tttatccggc actggtcggc aatgatatcg gctgcggtat
     4261 ggcgctatgg caaacagata ttctcgctcg caaatacaac gccgataagt ttgaaaagcg
     4321 attatcagat ctggatgacg ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc
     4381 atttgcacag catccgtggc gcagctcgct tggctctatc ggtggcggca atcatttcgc
     4441 tgaactgcaa caagttgatc aaatcatcaa cgctgaactg tttgcactgg caggtctgga
     4501 tgcacaacat ctgcaactgt tggttcatag cggctcgcgg ggggtggggc agtctatttt
     4561 acagcggcat attgcctcgt tctcgcatca tggtttgcct caaggcagtg acgacgcgct
     4621 acggtatatt gcggaacatg acgatgcgct ggcgtttgcg tgtattaatc gccagatgat
     4681 cgctttgcgc ataatgcaac aagttaaggc cacgggaagt ccggttctgg atgtggcgca
     4741 taactttgtt agcgcgtgtc aaatcggcga tcaacagggc tggttgcatc gtaaaggtgc
     4801 cacgccggat gacaacggtc tggtgattat tcccggttca cgtggtgatt actcctggat
     4861 tgtacagcat gttgcgaacg aaaaaacgct ccactcgctg gcgcatgggg cagggcgtaa
     4921 atgggggcgc accgagtgta aagggcgtct ggcagcgaaa tacacggtga cgcaactctc
     4981 gcggactgaa cttggcagtc gggtaatttg tcgcgataaa caactcatct ttgaagaagc
     5041 gccacaagcc tataaatcgg ctgaaagcgt ggtgcaatgt ctggtgcagg ccggtttaat
     5101 tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa aacagtggag ggaaaaaagg
     5161 atgatcttgc tacaactctc ctcagcgcag gggccggaag aatgttgtct cgcagtgaaa
     5221 aaggcgctgg agcgattaat taaagaagct gcccgacaag acgtcgcggt aacggtgttg
     5281 gaaacagaaa cgggccgcta ctctgacacg ctgcgttcag ctctggtttc tctggatggc
     5341 gataacgcat gggcattaag cgaaagctgg tgcggcacta ttcagtggat ttgtccgagt
     5401 ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg gcattgggcg ttttaccgct
     5461 gatgagcagg aacaatcgga tgcaatccgt tatgagacgt tgcgttcgtc ggggccgggc
     5521 ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatttggc atccggtatt
     5581 agcgtgaagg ttcagtcaga gcgtagtcag catgctaaca agcggctggc acgattgctg
     5641 attgcctgga agctggagca gcagcaacag gaaaatagcg cggtgctgaa atcgcagcgg
     5701 cgaatgttcc atcaccagat tgaacgtggc aacccgagac ggacatttac agggatggct
     5761 tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc atccggcagc
     5821 aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag tgtccagtaa
     5881 tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt gatagttggc
     5941 ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca ttccaggccc
     6001 gcgtggataa tctggatgtt cggtgttttg ttgaacagac gctgataggt ttcacgtacc
     6061 agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc tttcgcttcg
     6121 gttttcgcgc cagctagttt acccagcgaa tccagcatgc tcaccacgta gtctttaccg
     6181 ctgtcgatca gtgaacggat caggcagtga atttctacgt tattgtcagt catggtcacc
     6241 acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtcggaatt gcggatcaca
     6301 ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc aatcagggca
     6361 gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt ctctgccagc
     6421 tcgtttttca ggatctcctg ataggtattc accagagatt tcaggacgtc gactttatca
     6481 gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg
     6541 ttgaaatcga taaggcgcag atccagttct tccgcatgac ccgccaggaa gcgcaccagt
     6601 agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc gcctttcaga
     6661 ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc
     6721 aggttggagg tgaagtcgat accccccgca caacccatgt agatttcacc ttcttcttcg
     6781 gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc gaacgcaccg
     6841 tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg aaccacgttt
     6901 tcgtcagcca gaactgccag tgcagaggcc ataccaatgc cgttatccgc acccagcgtg
     6961 gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc tttcgtgaag
     7021 tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg taagacgacc
     7081 ggtttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt acctacctga
     7141 tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc agcgagttgc
     7201 tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc ccacagcggc
     7261 tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac
     7321 agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg cttgcgagat
     7381 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     7441 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     7501 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     7561 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     7621 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggtgc
     7681 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     7741 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     7801 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     7861 tccaaaagcg cactttgtca ccatctttgc aaaaccggct ggtcgtccgc tggttgatga
     7921 ttatgttgtt gatatcccgc aagatacctg gatcgaacag ccgtgggata tgggcgtcgt
     7981 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     8041 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     8101 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     8161 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg
     8221 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     8281 ttgacccacg tgaaatcctc gacgtccagg cacgtattgt gatgagcgat gctgaacgta
     8341 ccgatgatga tttatacgat accgtgattg gctaccgtgg tggcaactgg atttatgagt
     8401 gggccaccca ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca
     8461 gtggtcgaca ctggctgcat gcggctacgt tgtacaacat tgccgcctat cctcatctga
     8521 aaggagatga cctggccgag caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg
     8581 cccagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc ggtgcgccca
     8641 tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg
     8701 gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat tttgcgccgc
     8761 gcggcattgc gatgctgact attgatatgc tgtcggtggg cttttcttca aaatggaagc
     8821 tcacccagga ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt
     8881 gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac gttgccgtgc
     8941 gtctggcata ccttgaatcg ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc
     9001 ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc
     9061 tggcgagtcg tttggggatg catgatgctt ccgatgacgc gttgcgcgtg gagctgaatc
     9121 gctattcatt aaaagtgcaa
//
LOCUS       DAJSKJ010000467         6787 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.guided.188, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000467 DAJSKJ010000000
VERSION     DAJSKJ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.guided.188"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            164..604
                     /locus_tag="ON345_004108"
     CDS             164..604
                     /locus_tag="ON345_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR3074049.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(1349..1498)
                     /locus_tag="ON345_004109"
     CDS             complement(1349..1498)
                     /locus_tag="ON345_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3074050.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMTQVTEENLHAPVDFGSPV
                     GREAL"
     gene            2687..3502
                     /gene="sul2"
                     /locus_tag="ON345_004110"
     CDS             2687..3502
                     /gene="sul2"
                     /locus_tag="ON345_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR3074051.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3563..4366
                     /locus_tag="ON345_004111"
                     /note="aph(3'')-Ib"
     CDS             3563..4366
                     /locus_tag="ON345_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR3074052.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4366..5202
                     /locus_tag="ON345_004112"
     CDS             4366..5202
                     /locus_tag="ON345_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR3074053.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            5223..5696
                     /gene="dfrA"
                     /locus_tag="ON345_004113"
                     /pseudo
     CDS             5223..5696
                     /gene="dfrA"
                     /locus_tag="ON345_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
     gene            complement(5766..5891)
                     /locus_tag="ON345_004114"
                     /pseudo
     CDS             complement(5766..5891)
                     /locus_tag="ON345_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(5886..6290)
                     /locus_tag="ON345_004115"
                     /pseudo
     CDS             complement(5886..6290)
                     /locus_tag="ON345_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6334..6723)
                     /locus_tag="ON345_004116"
     CDS             complement(6334..6723)
                     /locus_tag="ON345_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3074054.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
BASE COUNT         1692 a         1763 c         1742 g         1590 t
ORIGIN      
        1 gtttatatac agtattcgaa atactacgcc cgtcaaaacc acaccgtcaa caggtttttt
       61 gtgtattttt cacgcctcac gggcagcctc agaccttttg tgttaaaaca gtaatatgtt
      121 ctgctgttac aatacggtac acacaggaga cactgtcact tttatggcat ggcacacagt
      181 tcaagagaca ctgaaattaa caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa
      241 aggccttgtc agctatcgca caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact
      301 gattcgggcc tatggcgagc tgcgcacttc tgagacacca aaaaggcaca aggagggaca
      361 ctccaaatct gtcgttagcc aggcggaaaa tgctcagcta gacgtcattc agcagaaact
      421 ggacgctctg cagcagactg ttaccctaat gcttgaagac aagatggcgc gggaagcaga
      481 aaaccgcctg cgaaatgagg agagggaaca actacaggcc gaaattagcc gtctggctga
      541 cgcgctggca caggaaaaaa agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga
      601 gtaacagtgc aggtgactct gatttaccat aacatctgtt atcagcaccg cctgagaggc
      661 cctcagggcg tttctgacgg gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc
      721 cagaaacgcg caggctctgt ttttacggca tgcatgagta tgcgcacaag tgcatagcgc
      781 ggaaattcac accattcagc cggggtaagt cctgtttccg gcgttcttcc catccgaaaa
      841 cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa
      901 cccgctgtca gcacgttcag gaagggggtg atgtacaaat ggcacagagg atgaaagtga
      961 cgtatgagct gaaggaatga tgtaaaagtg atgtataagt gatgtaaaag tgatgtatga
     1021 actgaaggag cgatgtaatt ctgtagtcag gaagaataac ccgttcatgc aggcatgaac
     1081 gatacagtga ggaacgcttt ttttgtcacg atctcccctt aacctgcgta aatcacccgc
     1141 catggttgat tatttttcaa cctcaaaatg gtcatacacc taacgcagcc gcgattttca
     1201 tcgcaactgc atgaattaaa agaaaaaaaa ggaatatttc gttttcacga gtaatacccg
     1261 ggtattaaaa atccactgta attacatgaa aataaagaaa taaaaggtaa taccggagta
     1321 ttaccacgta cgcgggcaag cgaaaaagct ataacgcttc ccggccgaca ggcgacccaa
     1381 aatccacagg ggcatgcagg ttctcttcag taacctgcgt cattaaatca aaaaggtcat
     1441 attcgccggg aagctgcgta tcagtcgaat ccagttgttc atcatattca gatggcatgg
     1501 ggtgatatcc agtagctgag aggttgttag cgggtttcgg ggcgcagccc tgaaccagtc
     1561 atgtagcgct agcggagtgt atactggctt actatgttgc cacgattgcg aaaatcagtg
     1621 aagtgcttca tgtggcagga gaaaaaaggc agcaccggcg cgtcagcaga atatgcgata
     1681 caggagatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg
     1741 agcggtaccg gcttacggac ggggacggaa atttcctgga agataccagg aagacactta
     1801 gcaaagaaga gataaggcca cggcgaagcc gtttttccat aggctccgcc cccctgacaa
     1861 gcatcgcaaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagatc
     1921 ccaggcgttt ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     1981 cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgacac tcagttccgt
     2041 gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     2101 cgccttgtcc ggtaactatc aacttgagtc caacccggaa agacacgaca aaacgccact
     2161 ggcagcagcc attagtaaca ggagtgcacg agaagataga ttaagaggtc ttgaagtaga
     2221 ggcctgactg cggctacact gaaagaacag ttttggtaat ggcggtctcg tgaaccctgt
     2281 taccgggtta agcagttccc caactgactt aaccttcgaa caaaccgcct ccccaggcgg
     2341 ttttttcgtt tacagagcaa gagattacga ttcattgaaa aggatctcaa gaagatcctt
     2401 tgtagattac tatcaattaa tcttccgaac atgacaccag tcactgttga ttacggttaa
     2461 accgctccgt aacaggtttc cctcgtattg gtgacttgca gcaccaaaga agcaaaaaag
     2521 ataaatatat ctacttattg agagatgata tatttatttc caaaatgcta cagatacatc
     2581 atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc
     2641 atccgtatga gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct
     2701 catcattttc ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct
     2761 ggcgccagac gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat
     2821 cgacctcggt ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat
     2881 cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga
     2941 cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga
     3001 tattcgcggt tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact
     3061 cgtcgttatg cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga
     3121 catcatggat cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg
     3181 tatcaaacgc aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc
     3241 cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc
     3301 ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg
     3361 ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt
     3421 catccgcaca cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa
     3481 agaaaccgca agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat
     3541 cagaaaactg aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc
     3601 tgactggttg cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg
     3661 gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga
     3721 ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga
     3781 ggaacaggag ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct
     3841 gtctggagcg gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca
     3901 cagcctatcg gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc
     3961 cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag
     4021 tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca
     4081 agagcgcacc gatatggttg tttgccatgg tgatccctgc atgccgaact tcatggtgga
     4141 ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa cagcagatcg
     4201 ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag cgccagatga
     4261 agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc ccgaccgcga
     4321 acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt catgccgcct
     4381 gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac cttttccagc
     4441 ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaaggg attgaaacct
     4501 atagaagaca ttgctgatga actgcgcggg gccgactatc tggtatggcg caatgggagg
     4561 ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata tgccggggag
     4621 cgaatgctct ctcacatcgt tgccgagcac ggcgactacc aggcgaccga aattgcagcg
     4681 gaactaatgg cgaagctgta tgccgcatct gaggaacccc tgccttctgc ccttctcccg
     4741 atccgggatc gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt
     4801 caaactgact acgtccacgc ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa
     4861 ctgcgtgggc tacatggcga tctgcatcat gaaaacatca tgttctccag tcgcggctgg
     4921 ctggtgatag atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc caatatgttc
     4981 tacgatccgg ctgacagaga cgacctttgt ctcgatccta gacgcattgc acagatggcg
     5041 gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct
     5101 tatgggtgcc tttccgcagc ttggaacgcg gatggagaag aggagcaacg cgatctagct
     5161 atcgcggccg cgatcaagca ggtgcgacag acgtcatact agttaacctc tgaggaagaa
     5221 ttgtgaaact atcactaatg gtagctatat cgaagaatgg agttatcggg aatggccctg
     5281 atattccatg gagtgccaaa ggtgaacagc tcctgtttaa agctattacc tataaccaat
     5341 ggctgttggt tggacgcaag acttttgaat caatgggagc attacccaac cgaaagtatg
     5401 cggtcgtaac acgttcaagt tttacatctg acaatgagaa cgtattgatc tttccatcaa
     5461 ttaaagatgc ttaaaccaac ctaaagaaaa taacggatca tgtcattgtt tcaggtggtg
     5521 gggagatata caaaagcctg atcgatcaag tagatacact acatatatct acaatagaca
     5581 tcgagccgga aggtgatgtt tactttcctg aaatccccag caattttagg ccagttttta
     5641 cccaagactt cgcctctaac ataaattata gttaccaaat ctggcaaaag ggttaacaag
     5701 tggcagcaac ggattcgcaa acctgtcacg ccttttgtgc caaaagccgc gccaggtttg
     5761 cgatccgctg tgccaggcga tatcaagcga cttctcctat cccctgggaa cacatcaatc
     5821 tcaccggaga atatcgctgg ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg
     5881 accccaaaca ggaaacgcag ctgaaacggg aagctcaaca cccactgacg catgggttgt
     5941 tcaggcagta cttcatcaac cagcaaggcg gcactttcgg ccatccgccg cgccccacag
     6001 ctcgggcaga aaccgcgacg cttacagctg aaagcgacca ggtgctcggc gtggcaagac
     6061 tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat
     6121 tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc agcgaatgcc
     6181 gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa
     6241 cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc
     6301 cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc
     6361 cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactccca ctcttacatt
     6421 gttcgcagta tctaggtaca tgttattgcg tgcaccttca tgatgtataa tgttattaat
     6481 gacagcagaa agatcgacag aattaatatt cttcgtacct gggacagtta tcgggtgtcg
     6541 gccagtgatg agaccaaagg atgcagtata agatgaacca ctactattat catggccacc
     6601 attaccacca ccgttaaaac tacctcccga acttccagag ccgctatgac catcattgta
     6661 ggggtcattt ggtcccatgt gaccaaaacc accgacagta ccgctattta actctgtact
     6721 cataaaatta tcctctttga cttttaaaaa cagcaaaaac tgtatttata acaatgtgtt
     6781 tatatac
//
LOCUS       DAJSKJ010000468         6787 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.guided.189, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000468 DAJSKJ010000000
VERSION     DAJSKJ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.guided.189"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            164..604
                     /locus_tag="ON345_004117"
     CDS             164..604
                     /locus_tag="ON345_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR3074055.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(1349..1498)
                     /locus_tag="ON345_004118"
     CDS             complement(1349..1498)
                     /locus_tag="ON345_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3074056.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMTQVTEENLHAPVDFGSPV
                     GREAL"
     gene            2687..3502
                     /gene="sul2"
                     /locus_tag="ON345_004119"
     CDS             2687..3502
                     /gene="sul2"
                     /locus_tag="ON345_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR3074057.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3563..4366
                     /locus_tag="ON345_004120"
                     /note="aph(3'')-Ib"
     CDS             3563..4366
                     /locus_tag="ON345_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR3074058.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4366..5202
                     /locus_tag="ON345_004121"
     CDS             4366..5202
                     /locus_tag="ON345_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR3074059.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            5223..5696
                     /locus_tag="ON345_004122"
     CDS             5223..5696
                     /locus_tag="ON345_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR3074060.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            complement(5886..6290)
                     /locus_tag="ON345_004123"
                     /pseudo
     CDS             complement(5886..6290)
                     /locus_tag="ON345_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6334..6723)
                     /locus_tag="ON345_004124"
     CDS             complement(6334..6723)
                     /locus_tag="ON345_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3074061.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
BASE COUNT         1691 a         1763 c         1742 g         1591 t
ORIGIN      
        1 gtttatatac agtattcgaa atactacgcc cgtcaaaacc acaccgtcaa caggtttttt
       61 gtgtattttt cacgcctcac gggcagcctc agaccttttg tgttaaaaca gtaatatgtt
      121 ctgctgttac aatacggtac acacaggaga cactgtcact tttatggcat ggcacacagt
      181 tcaagagaca ctgaaattaa caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa
      241 aggccttgtc agctatcgca caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact
      301 gattcgggcc tatggcgagc tgcgcacttc tgagacacca aaaaggcaca aggagggaca
      361 ctccaaatct gtcgttagcc aggcggaaaa tgctcagcta gacgtcattc agcagaaact
      421 ggacgctctg cagcagactg ttaccctaat gcttgaagac aagatggcgc gggaagcaga
      481 aaaccgcctg cgaaatgagg agagggaaca actacaggcc gaaattagcc gtctggctga
      541 cgcgctggca caggaaaaaa agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga
      601 gtaacagtgc aggtgactct gatttaccat aacatctgtt atcagcaccg cctgagaggc
      661 cctcagggcg tttctgacgg gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc
      721 cagaaacgcg caggctctgt ttttacggca tgcatgagta tgcgcacaag tgcatagcgc
      781 ggaaattcac accattcagc cggggtaagt cctgtttccg gcgttcttcc catccgaaaa
      841 cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa
      901 cccgctgtca gcacgttcag gaagggggtg atgtacaaat ggcacagagg atgaaagtga
      961 cgtatgagct gaaggaatga tgtaaaagtg atgtataagt gatgtaaaag tgatgtatga
     1021 actgaaggag cgatgtaatt ctgtagtcag gaagaataac ccgttcatgc aggcatgaac
     1081 gatacagtga ggaacgcttt ttttgtcacg atctcccctt aacctgcgta aatcacccgc
     1141 catggttgat tatttttcaa cctcaaaatg gtcatacacc taacgcagcc gcgattttca
     1201 tcgcaactgc atgaattaaa agaaaaaaaa ggaatatttc gttttcacga gtaatacccg
     1261 ggtattaaaa atccactgta attacatgaa aataaagaaa taaaaggtaa taccggagta
     1321 ttaccacgta cgcgggcaag cgaaaaagct ataacgcttc ccggccgaca ggcgacccaa
     1381 aatccacagg ggcatgcagg ttctcttcag taacctgcgt cattaaatca aaaaggtcat
     1441 attcgccggg aagctgcgta tcagtcgaat ccagttgttc atcatattca gatggcatgg
     1501 ggtgatatcc agtagctgag aggttgttag cgggtttcgg ggcgcagccc tgaaccagtc
     1561 atgtagcgct agcggagtgt atactggctt actatgttgc cacgattgcg aaaatcagtg
     1621 aagtgcttca tgtggcagga gaaaaaaggc agcaccggcg cgtcagcaga atatgcgata
     1681 caggagatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg
     1741 agcggtaccg gcttacggac ggggacggaa atttcctgga agataccagg aagacactta
     1801 gcaaagaaga gataaggcca cggcgaagcc gtttttccat aggctccgcc cccctgacaa
     1861 gcatcgcaaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagatc
     1921 ccaggcgttt ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     1981 cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgacac tcagttccgt
     2041 gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     2101 cgccttgtcc ggtaactatc aacttgagtc caacccggaa agacacgaca aaacgccact
     2161 ggcagcagcc attagtaaca ggagtgcacg agaagataga ttaagaggtc ttgaagtaga
     2221 ggcctgactg cggctacact gaaagaacag ttttggtaat ggcggtctcg tgaaccctgt
     2281 taccgggtta agcagttccc caactgactt aaccttcgaa caaaccgcct ccccaggcgg
     2341 ttttttcgtt tacagagcaa gagattacga ttcattgaaa aggatctcaa gaagatcctt
     2401 tgtagattac tatcaattaa tcttccgaac atgacaccag tcactgttga ttacggttaa
     2461 accgctccgt aacaggtttc cctcgtattg gtgacttgca gcaccaaaga agcaaaaaag
     2521 ataaatatat ctacttattg agagatgata tatttatttc caaaatgcta cagatacatc
     2581 atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc
     2641 atccgtatga gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct
     2701 catcattttc ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct
     2761 ggcgccagac gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat
     2821 cgacctcggt ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat
     2881 cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga
     2941 cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga
     3001 tattcgcggt tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact
     3061 cgtcgttatg cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga
     3121 catcatggat cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg
     3181 tatcaaacgc aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc
     3241 cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc
     3301 ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg
     3361 ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt
     3421 catccgcaca cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa
     3481 agaaaccgca agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat
     3541 cagaaaactg aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc
     3601 tgactggttg cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg
     3661 gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga
     3721 ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga
     3781 ggaacaggag ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct
     3841 gtctggagcg gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca
     3901 cagcctatcg gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc
     3961 cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag
     4021 tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca
     4081 agagcgcacc gatatggttg tttgccatgg tgatccctgc atgccgaact tcatggtgga
     4141 ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa cagcagatcg
     4201 ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag cgccagatga
     4261 agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc ccgaccgcga
     4321 acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt catgccgcct
     4381 gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac cttttccagc
     4441 ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaaggg attgaaacct
     4501 atagaagaca ttgctgatga actgcgcggg gccgactatc tggtatggcg caatgggagg
     4561 ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata tgccggggag
     4621 cgaatgctct ctcacatcgt tgccgagcac ggcgactacc aggcgaccga aattgcagcg
     4681 gaactaatgg cgaagctgta tgccgcatct gaggaacccc tgccttctgc ccttctcccg
     4741 atccgggatc gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt
     4801 caaactgact acgtccacgc ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa
     4861 ctgcgtgggc tacatggcga tctgcatcat gaaaacatca tgttctccag tcgcggctgg
     4921 ctggtgatag atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc caatatgttc
     4981 tacgatccgg ctgacagaga cgacctttgt ctcgatccta gacgcattgc acagatggcg
     5041 gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct
     5101 tatgggtgcc tttccgcagc ttggaacgcg gatggagaag aggagcaacg cgatctagct
     5161 atcgcggccg cgatcaagca ggtgcgacag acgtcatact agttaacctc tgaggaagaa
     5221 ttgtgaaact atcactaatg gtagctatat cgaagaatgg agttatcggg aatggccctg
     5281 atattccatg gagtgccaaa ggtgaacagc tcctgtttaa agctattacc tataaccaat
     5341 ggctgttggt tggacgcaag acttttgaat caatgggagc attacccaac cgaaagtatg
     5401 cggtcgtaac acgttcaagt tttacatctg acaatgagaa cgtattgatc tttccatcaa
     5461 ttaaagatgc tttaaccaac ctaaagaaaa taacggatca tgtcattgtt tcaggtggtg
     5521 gggagatata caaaagcctg atcgatcaag tagatacact acatatatct acaatagaca
     5581 tcgagccgga aggtgatgtt tactttcctg aaatccccag caattttagg ccagttttta
     5641 cccaagactt cgcctctaac ataaattata gttaccaaat ctggcaaaag ggttaacaag
     5701 tggcagcaac ggattcgcaa acctgtcacg ccttttgtgc caaaagccgc gccaggtttg
     5761 cgatccgctg tgccaggcga tatcaagcga cttctcctat cccctgggaa cacatcaatc
     5821 tcaccggaga atatcgctgg ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg
     5881 accccaaaca ggaaacgcag ctgaaacggg aagctcaaca cccactgacg catgggttgt
     5941 tcaggcagta cttcatcaac cagcaaggcg gcactttcgg ccatccgccg cgccccacag
     6001 ctcgggcaga aaccgcgacg cttacagctg aaagcgacca ggtgctcggc gtggcaagac
     6061 tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat
     6121 tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc agcgaatgcc
     6181 gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa
     6241 cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc
     6301 cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc
     6361 cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactccca ctcttacatt
     6421 gttcgcagta tctaggtaca tgttattgcg tgcaccttca tgatgtataa tgttattaat
     6481 gacagcagaa agatcgacag aattaatatt cttcgtacct gggacagtta tcgggtgtcg
     6541 gccagtgatg agaccaaagg atgcagtata agatgaacca ctactattat catggccacc
     6601 attaccacca ccgttaaaac tacctcccga acttccagag ccgctatgac catcattgta
     6661 ggggtcattt ggtcccatgt gaccaaaacc accgacagta ccgctattta actctgtact
     6721 cataaaatta tcctctttga cttttaaaaa cagcaaaaac tgtatttata acaatgtgtt
     6781 tatatac
//
LOCUS       DAJSKJ010000469         2177 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.guided.288, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000469 DAJSKJ010000000
VERSION     DAJSKJ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.guided.288"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            114..974
                     /locus_tag="ON345_004125"
     CDS             114..974
                     /locus_tag="ON345_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase SHV-12"
                     /protein_id="HCR3074062.1"
                     /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIE
                     MDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYS
                     PVSEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDR
                     WETELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIR
                     SVLPAGWFIADKTGASKRGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGI
                     GAALIEHWQR"
     gene            complement(995..1708)
                     /locus_tag="ON345_004126"
     CDS             complement(995..1708)
                     /locus_tag="ON345_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017349110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3074063.1"
                     /translation="MDLRPLNELTEILGVSHMTIRRDVSKLEEQGLLVSVSGGVRAVS
                     RLAAEPSHLVKSTLQSEEKQAIGALAASHIAKNSCIYLDAGTTTLALARAILDRNDLQ
                     VVTNDFEITQLLIDASQCGVIHTGGTLCRENRSCVGESAARTLRHLAIDTAFISASGW
                     DSRGIFTPDENKVTVKETVSQVSARSILLCDSSKYNQVATFMALPLTRFTTIITDRHL
                     SDAAASHIARHACEVLRAG"
     gene            1957..2136
                     /locus_tag="ON345_004127"
                     /pseudo
     CDS             1957..2136
                     /locus_tag="ON345_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          448 a          641 c          642 g          446 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aacgccgggt tattcttatt
       61 tgtcgcttct ttactcgcct ttatcggcct tcactcaagg atgtattgtg gttatgcgtt
      121 atattcgcct gtgtattatc tccctgttag ccaccctgcc gctggcggta cacgccagcc
      181 cgcagccgct tgagcaaatt aaacaaagcg aaagccagct gtcgggccgc gtaggcatga
      241 tagaaatgga tctggccagc ggccgcacgc tgaccgcctg gcgcgccgat gaacgctttc
      301 ccatgatgag cacctttaaa gtagtgctct gcggcgcagt gctggcgcgg gtggatgccg
      361 gtgacgaaca gctggagcga aagatccact atcgccagca ggatctggtg gactactcgc
      421 cggtcagcga aaaacacctt gccgacggca tgacggtcgg cgaactctgc gccgccgcca
      481 ttaccatgag cgataacagc gccgccaatc tgctgctggc caccgtcggc ggccccgcag
      541 gattgactgc ctttttgcgc cagatcggcg acaacgtcac ccgccttgac cgctgggaaa
      601 cggaactgaa tgaggcgctt cccggcgacg cccgcgacac cactaccccg gccagcatgg
      661 ccgcgaccct gcgcaagctg ctgaccagcc agcgtctgag cgcccgttcg caacggcagc
      721 tgctgcagtg gatggtggac gatcgggtcg ccggaccgtt gatccgctcc gtgctgccgg
      781 cgggctggtt tatcgccgat aagaccggag ctagcaagcg gggtgcgcgc gggattgtcg
      841 ccctgcttgg cccgaataac aaagcagagc gcattgtggt gatttatctg cgggatacgc
      901 cggcgagcat ggccgagcga aatcagcaaa tcgccgggat cggcgcggcg ctgatcgagc
      961 actggcaacg ctaagccggc ggtggccgcg cgcgttatcc ggcccgcagc acctcgcagg
     1021 cgtgccgggc gatatgactg gcggcggcat cggaaagatg ccggtcggta atgatggtgg
     1081 tgaaccgggt caaaggtaac gccataaacg tggccacctg attgtatttc gaactgtcgc
     1141 acagcaggat gcttctcgcg ctgacctggc tgacggtctc cttgacggta accttgttct
     1201 catcaggggt gaatatcccg cgactgtccc agccgctggc ggagataaag gccgtatcga
     1261 tagccaggtg gcgtaacgta cgcgccgccg attcgcccac gcaggagcgg ttctcccggc
     1321 acagagtgcc gccggtgtgg atcacgccgc actggctggc atcgatcagc agctgggtta
     1381 tctcaaaatc attggtgacc acctggagat cgttccggtc gaggatcgcc cgcgccagcg
     1441 ccagggtggt agtcccggca tccagataga tgcaactgtt tttagcgata tgactcgccg
     1501 ccagcgcgcc gatcgcctgt ttctcctcac tctgcagcgt gcttttcacc agatgactgg
     1561 gttccgcggc cagccggctg acggcgcgta cgccgcccga gacgctgacc agcagcccct
     1621 gctcctccag tttactgacg tcccgacgga tggtcatatg ggacacgccg aggatctccg
     1681 tcagctcgtt aaggggtctg aggtccaacc atgcgaaaaa ccacgttaag aaagcaacct
     1741 tctgaaaagg cgacgtttat cagttttttt tccggccata aagggggcgg aaattattcc
     1801 ctgcacgggt attcgcaagg atttcggtag tgctctggct ctgcccccca taacctggcg
     1861 gacagagcat gattgggaaa gcggtaccct cacggcagaa acgattgacg caggctatat
     1921 ttctattatt ctggaaatat agataaagga ggtattatgg atttaaacac tgctgcaaac
     1981 gcacttcgag aactgggcca cccgacacgt ctcagcatat accgggagct ggtcagggcg
     2041 ggtcatgaag gcctgccggt tggcgaactg cagaagcacc ttgagattcc tgcctccacg
     2101 ctcagccatc atctttcagc gctgatatcc gctggacggc actgttgcaa atagtcggtg
     2161 gtgataaact tatcatc
//
LOCUS       DAJSKJ010000470         1293 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Escherichia coli isolate IRO-J42_5
            SAMN07177836-rid15778183.guided.324, whole genome shotgun sequence.
ACCESSION   DAJSKJ010000470 DAJSKJ010000000
VERSION     DAJSKJ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07177836
            Sequence Read Archive: SRR5679633
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001443013.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001443013.2
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 22:46:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,128
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 3,937
            CDSs (with protein)               :: 3,937
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 35 of 126
            Pseudo Genes (incomplete)         :: 64 of 126
            Pseudo Genes (internal stop)      :: 37 of 126
            Pseudo Genes (multiple problems)  :: 9 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07177836-rid15778183.guided.324"
                     /isolate="IRO-J42_5"
                     /isolation_source="cell culture"
                     /db_xref="taxon:562"
                     /country="Guadeloupe"
                     /lat_lon="16.26 N 61.56 W"
                     /collection_date="2015"
                     /collected_by="Pasteur Institute of Guadeloupe"
     gene            493..1149
                     /locus_tag="ON345_004128"
     CDS             493..1149
                     /locus_tag="ON345_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HCR3074064.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
BASE COUNT          350 a          289 c          279 g          375 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ttttttcctg cggtgtacga
       61 cgccgacgcc gctcaggtcc tgataacact ccaaccatct tatcgttctg actggtatta
      121 aacatagttc caagactacc tcttatttta agagagtcga agtgtctggt gatctatggg
      181 gccagtctag ccctcttcca agtgtaggga gatatagtca aagtttgctt ctccgccgat
      241 gagtccgccc tgcgtcggat agtccgattt tttaccggcg aagcacggct ctcctaaccc
      301 tatctaaagc gaataacttt tttcaacagg gccgcgtgcc aatgcaagag cgataggcgc
      361 tctacgcgcc aatttgacca cttaaaacag gtaaattgag gggttgtaat gtgttgatgt
      421 aacaggcttt tattttaatg tcttggcata tgtataatgg tagtctagcc ctcctttcaa
      481 caaggagtac tcatggaaac ctacaatcat acatatcggc accacaactt ttcacataaa
      541 gacttaagtg atctcacctt caccgcttgc acattcattc gcagcgactt tcgacgtgct
      601 aacttgcgtg atacgacatt cgtcaactgc aagttcattg aacagggtga tatcgaaggc
      661 tgccactttg atgtcgcaga tcttcgtgat gcaagtttcc aacaatgcca acttgcgatg
      721 gcaaacttca gtaatgccaa ttgctacggt atagagttcc gtgcgtgtga tttaaaaggt
      781 gccaactttt cccgaacaaa ctttgcccat caagtgagta atcgtatgta cttttgctca
      841 gcatttattt ctggatgtaa tctttcctat gccaatatgg agagggtttg tttagaaaaa
      901 tgtgagttgt ttgaaaatcg ctggatagga acgaacctag cgggtgcatc actgaaagag
      961 tcagacttaa gtcgaggtgt tttttccgaa gatgtctggg ggcaatttag cctacagggt
     1021 gccaatttat gccacgccga actcgacggt ttagatcccc gcaaagtcga tacatcaggt
     1081 atcaaaattg cagcctggca gcaagaactg attctcgaag cactgggtat tgttgtttat
     1141 cctgactaat tgctttgatg tgtgatttta aacgctcaaa tttataaaac gaataatatt
     1201 gtcaaccttg caataataga catagggaca accatgtata aatttttacc ctcaggcgtc
     1261 ttcacaatag agccatatag cagtgggtca ctt
//
